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Id Subject Object Predicate Lexical cue
T78 222-227 JJ denotes fatty
T79 228-232 NN denotes acid
T80 233-234 NN denotes β
T81 234-235 HYPH denotes -
T82 245-247 IN denotes in
T83 248-254 NNS denotes humans
T84 254-255 . denotes .
T85 255-415 sentence denotes To better understand the pathogenesis of this disease, we developed a mouse model for MCAD deficiency (MCAD−/−) by gene targeting in embryonic stem (ES) cells.
T86 256-258 TO denotes To
T87 266-276 VB denotes understand
T88 259-265 RBR denotes better
T89 314-323 VBD denotes developed
T90 277-280 DT denotes the
T91 281-293 NN denotes pathogenesis
T92 294-296 IN denotes of
T93 297-301 DT denotes this
T94 302-309 NN denotes disease
T95 309-311 , denotes ,
T96 311-313 PRP denotes we
T97 324-325 DT denotes a
T98 332-337 NN denotes model
T99 326-331 NN denotes mouse
T100 338-341 IN denotes for
T101 342-346 NN denotes MCAD
T102 347-357 NN denotes deficiency
T103 358-359 -LRB- denotes (
T104 359-363 NN denotes MCAD
T105 363-364 SYM denotes −
T106 364-365 HYPH denotes /
T107 365-366 SYM denotes −
T108 366-367 -RRB- denotes )
T109 368-370 IN denotes by
T110 371-375 NN denotes gene
T111 376-385 NN denotes targeting
T112 386-388 IN denotes in
T113 389-398 JJ denotes embryonic
T114 399-403 NN denotes stem
T115 409-414 NNS denotes cells
T116 404-405 -LRB- denotes (
T117 405-407 NN denotes ES
T118 407-408 -RRB- denotes )
T119 414-415 . denotes .
T120 415-544 sentence denotes The MCAD−/− mice developed an organic aciduria and fatty liver, and showed profound cold intolerance at 4 °C with prior fasting.
T121 416-419 DT denotes The
T152 609-617 VBN denotes reported
T153 574-578 VBN denotes seen
T154 579-581 IN denotes in
T155 582-586 NN denotes MCAD
T156 590-594 NNS denotes mice
T157 586-587 SYM denotes −
T158 587-588 HYPH denotes /
T159 588-589 SYM denotes −
T160 595-599 VBP denotes have
T161 600-603 RB denotes not
T162 604-608 VBN denotes been
T163 618-620 IN denotes in
T164 621-626 JJ denotes human
T165 632-640 NNS denotes patients
T166 627-631 NN denotes MCAD
T167 640-641 . denotes .
T168 641-800 sentence denotes There was significant neonatal mortality of MCAD−/− pups demonstrating similarities to patterns of clinical episodes and mortality in MCAD-deficient patients.
T169 642-647 EX denotes There
T170 648-651 VBD denotes was
T171 652-663 JJ denotes significant
T172 673-682 NN denotes mortality
T173 664-672 JJ denotes neonatal
T174 683-685 IN denotes of
T175 686-690 NN denotes MCAD
T176 694-698 NNS denotes pups
T177 690-691 SYM denotes −
T178 691-692 HYPH denotes /
T179 692-693 SYM denotes −
T180 699-712 VBG denotes demonstrating
T181 713-725 NNS denotes similarities
T182 726-728 IN denotes to
T183 729-737 NNS denotes patterns
T184 738-740 IN denotes of
T185 741-749 JJ denotes clinical
T186 750-758 NNS denotes episodes
T187 759-762 CC denotes and
T188 763-772 NN denotes mortality
T189 773-775 IN denotes in
T190 776-780 NN denotes MCAD
T191 781-790 JJ denotes deficient
T192 780-781 HYPH denotes -
T193 791-799 NNS denotes patients
T194 799-800 . denotes .
T195 800-1028 sentence denotes The MCAD-deficient mouse reproduced important aspects of human MCAD deficiency and is a valuable model for further analysis of the roles of fatty acid oxidation and pathogenesis of human diseases involving fatty acid oxidation.
T196 801-804 DT denotes The
T197 820-825 NN denotes mouse
T198 805-809 NN denotes MCAD
T199 810-819 JJ denotes deficient
T200 809-810 HYPH denotes -
T201 826-836 VBD denotes reproduced
T202 837-846 JJ denotes important
T203 847-854 NNS denotes aspects
T204 855-857 IN denotes of
T205 858-863 JJ denotes human
T206 869-879 NN denotes deficiency
T207 864-868 NN denotes MCAD
T208 880-883 CC denotes and
T209 884-886 VBZ denotes is
T210 887-888 DT denotes a
T211 898-903 NN denotes model
T212 889-897 JJ denotes valuable
T213 904-907 IN denotes for
T214 908-915 JJ denotes further
T215 916-924 NN denotes analysis
T216 925-927 IN denotes of
T217 928-931 DT denotes the
T218 932-937 NNS denotes roles
T219 938-940 IN denotes of
T220 941-946 JJ denotes fatty
T221 947-951 NN denotes acid
T222 952-961 NN denotes oxidation
T223 962-965 CC denotes and
T224 966-978 NN denotes pathogenesis
T225 979-981 IN denotes of
T226 982-987 JJ denotes human
T227 988-996 NNS denotes diseases
T228 997-1006 VBG denotes involving
T229 1007-1012 JJ denotes fatty
T230 1013-1017 NN denotes acid
T231 1018-1027 NN denotes oxidation
T232 1027-1028 . denotes .
T373 2143-2156 JJ denotes Mitochondrial
T374 2159-2168 NN denotes oxidation
T375 2157-2158 NN denotes β
T376 2158-2159 HYPH denotes -
T377 2184-2192 VBZ denotes provides
T378 2169-2171 IN denotes of
T379 2172-2177 JJ denotes fatty
T380 2178-2183 NNS denotes acids
T381 2193-2199 NN denotes energy
T382 2199-2201 , denotes ,
T383 2201-2211 RB denotes especially
T384 2212-2218 IN denotes during
T385 2219-2226 NN denotes fasting
T386 2227-2237 NNS denotes conditions
T387 2237-2238 . denotes .
T388 2238-2344 sentence denotes Fatty acid oxidation occurs in mitochondria and consists of a repeating circuit of four sequential steps.
T389 2239-2244 JJ denotes Fatty
T390 2245-2249 NN denotes acid
T391 2250-2259 NN denotes oxidation
T392 2260-2266 VBZ denotes occurs
T393 2267-2269 IN denotes in
T394 2270-2282 NNS denotes mitochondria
T395 2283-2286 CC denotes and
T396 2287-2295 VBZ denotes consists
T397 2296-2298 IN denotes of
T398 2299-2300 DT denotes a
T399 2311-2318 NN denotes circuit
T400 2301-2310 VBG denotes repeating
T401 2319-2321 IN denotes of
T402 2322-2326 CD denotes four
T403 2338-2343 NNS denotes steps
T404 2327-2337 JJ denotes sequential
T405 2343-2344 . denotes .
T406 2344-2428 sentence denotes There are four straight-chain acyl-CoA dehydrogenases involved in the initial step.
T407 2345-2350 EX denotes There
T408 2351-2354 VBP denotes are
T409 2355-2359 CD denotes four
T410 2384-2398 NNS denotes dehydrogenases
T411 2360-2368 JJ denotes straight
T412 2369-2374 NN denotes chain
T413 2368-2369 HYPH denotes -
T414 2375-2379 NN denotes acyl
T415 2380-2383 NN denotes CoA
T416 2379-2380 HYPH denotes -
T417 2399-2407 VBN denotes involved
T418 2408-2410 IN denotes in
T419 2411-2414 DT denotes the
T420 2423-2427 NN denotes step
T421 2415-2422 JJ denotes initial
T422 2427-2428 . denotes .
T423 2428-2650 sentence denotes Medium-chain acyl-CoA dehydrogenase (MCAD) (the mouse gene is Acadm, whereas the protein is MCAD), specifically, is responsible for catalyzing the dehydrogenation of medium-chain length (C6–C12) fatty acid thioesters [1].
T424 2429-2435 JJ denotes Medium
T425 2436-2441 NN denotes chain
T426 2435-2436 HYPH denotes -
T427 2451-2464 NN denotes dehydrogenase
T428 2442-2446 NN denotes acyl
T429 2447-2450 NN denotes CoA
T430 2446-2447 HYPH denotes -
T431 2542-2544 VBZ denotes is
T432 2465-2466 -LRB- denotes (
T433 2466-2470 NN denotes MCAD
T434 2470-2471 -RRB- denotes )
T435 2472-2473 -LRB- denotes (
T436 2488-2490 VBZ denotes is
T437 2473-2476 DT denotes the
T438 2483-2487 NN denotes gene
T439 2477-2482 NN denotes mouse
T440 2491-2496 NN denotes Acadm
T441 2496-2498 , denotes ,
T442 2498-2505 IN denotes whereas
T443 2518-2520 VBZ denotes is
T444 2506-2509 DT denotes the
T445 2510-2517 NN denotes protein
T446 2521-2525 NN denotes MCAD
T447 2525-2526 -RRB- denotes )
T448 2526-2528 , denotes ,
T449 2528-2540 RB denotes specifically
T450 2540-2542 , denotes ,
T451 2545-2556 JJ denotes responsible
T452 2557-2560 IN denotes for
T453 2561-2571 VBG denotes catalyzing
T454 2572-2575 DT denotes the
T455 2576-2591 NN denotes dehydrogenation
T456 2592-2594 IN denotes of
T457 2595-2601 JJ denotes medium
T458 2602-2607 NN denotes chain
T459 2601-2602 HYPH denotes -
T460 2608-2614 NN denotes length
T461 2635-2645 NNS denotes thioesters
T462 2615-2616 -LRB- denotes (
T463 2619-2622 NN denotes C12
T464 2616-2618 NN denotes C6
T465 2618-2619 SYM denotes –
T466 2622-2623 -RRB- denotes )
T467 2624-2629 JJ denotes fatty
T468 2630-2634 NN denotes acid
T469 2646-2647 -LRB- denotes [
T470 2647-2648 CD denotes 1
T471 2648-2649 -RRB- denotes ]
T472 2649-2650 . denotes .
T473 2650-2768 sentence denotes Acadm is transcribed in the nucleus, translated in the cytosol, and translocated into the mitochondrial matrix [2–4].
T474 2651-2656 NN denotes Acadm
T475 2660-2671 VBN denotes transcribed
T476 2657-2659 VBZ denotes is
T477 2672-2674 IN denotes in
T478 2675-2678 DT denotes the
T479 2679-2686 NN denotes nucleus
T480 2686-2688 , denotes ,
T481 2688-2698 VBN denotes translated
T482 2699-2701 IN denotes in
T483 2702-2705 DT denotes the
T484 2706-2713 NN denotes cytosol
T485 2713-2715 , denotes ,
T486 2715-2718 CC denotes and
T487 2719-2731 VBN denotes translocated
T488 2732-2736 IN denotes into
T489 2737-2740 DT denotes the
T490 2755-2761 NN denotes matrix
T491 2741-2754 JJ denotes mitochondrial
T492 2762-2763 -LRB- denotes [
T493 2763-2764 CD denotes 2
T494 2764-2765 SYM denotes –
T495 2765-2766 CD denotes 4
T496 2766-2767 -RRB- denotes ]
T497 2767-2768 . denotes .
T498 2768-2889 sentence denotes Once inside the mitochondrial matrix, the MCAD monomers are assembled into homotetramers to gain enzymatic activity [4].
T499 2769-2773 IN denotes Once
T500 2774-2780 IN denotes inside
T501 2829-2838 VBN denotes assembled
T502 2781-2784 DT denotes the
T503 2799-2805 NN denotes matrix
T504 2785-2798 JJ denotes mitochondrial
T505 2805-2807 , denotes ,
T506 2807-2810 DT denotes the
T507 2816-2824 NNS denotes monomers
T508 2811-2815 NN denotes MCAD
T509 2825-2828 VBP denotes are
T510 2839-2843 IN denotes into
T511 2844-2857 NNS denotes homotetramers
T512 2858-2860 TO denotes to
T513 2861-2865 VB denotes gain
T514 2866-2875 JJ denotes enzymatic
T515 2876-2884 NN denotes activity
T516 2885-2886 -LRB- denotes [
T517 2886-2887 CD denotes 4
T518 2887-2888 -RRB- denotes ]
T519 2888-2889 . denotes .
T520 2889-2951 sentence denotes MCAD activity is essential for complete fatty acid oxidation.
T521 2890-2894 NN denotes MCAD
T522 2895-2903 NN denotes activity
T523 2904-2906 VBZ denotes is
T524 2907-2916 JJ denotes essential
T525 2917-2920 IN denotes for
T526 2921-2929 JJ denotes complete
T527 2941-2950 NN denotes oxidation
T528 2930-2935 JJ denotes fatty
T529 2936-2940 NN denotes acid
T530 2950-2951 . denotes .
T531 2951-3030 sentence denotes Inherited MCAD deficiency exists in humans as an autosomal recessive disorder.
T532 2952-2961 VBN denotes Inherited
T533 2967-2977 NN denotes deficiency
T534 2962-2966 NN denotes MCAD
T535 2978-2984 VBZ denotes exists
T536 2985-2987 IN denotes in
T537 2988-2994 NNS denotes humans
T538 2995-2997 IN denotes as
T539 2998-3000 DT denotes an
T540 3021-3029 NN denotes disorder
T541 3001-3010 JJ denotes autosomal
T542 3011-3020 JJ denotes recessive
T543 3029-3030 . denotes .
T544 3030-3139 sentence denotes MCAD deficiency was first described in 1982—1983 [5–7] and has been described in numerous patients [1,8–11].
T545 3031-3035 NN denotes MCAD
T546 3036-3046 NN denotes deficiency
T547 3057-3066 VBN denotes described
T548 3047-3050 VBD denotes was
T549 3051-3056 RB denotes first
T550 3067-3069 IN denotes in
T551 3070-3074 CD denotes 1982
T552 3074-3075 SYM denotes —
T553 3075-3079 CD denotes 1983
T554 3080-3081 -LRB- denotes [
T555 3081-3082 CD denotes 5
T556 3082-3083 SYM denotes –
T557 3083-3084 CD denotes 7
T558 3084-3085 -RRB- denotes ]
T559 3086-3089 CC denotes and
T560 3090-3093 VBZ denotes has
T561 3099-3108 VBN denotes described
T562 3094-3098 VBN denotes been
T563 3109-3111 IN denotes in
T564 3112-3120 JJ denotes numerous
T565 3121-3129 NNS denotes patients
T566 3130-3131 -LRB- denotes [
T567 3131-3132 CD denotes 1
T568 3132-3133 , denotes ,
T569 3133-3134 CD denotes 8
T570 3134-3135 SYM denotes –
T571 3135-3137 CD denotes 11
T572 3137-3138 -RRB- denotes ]
T573 3138-3139 . denotes .
T574 3139-3315 sentence denotes The carrier frequency in the Caucasian population has been estimated to be between 1 in 50 to 80 with an incidence of clinical disease expected at around 1 in 15,000 [1,9,12].
T575 3140-3143 DT denotes The
T576 3152-3161 NN denotes frequency
T577 3144-3151 NN denotes carrier
T578 3199-3208 VBN denotes estimated
T579 3162-3164 IN denotes in
T580 3165-3168 DT denotes the
T581 3179-3189 NN denotes population
T582 3169-3178 JJ denotes Caucasian
T583 3190-3193 VBZ denotes has
T584 3194-3198 VBN denotes been
T585 3209-3211 TO denotes to
T586 3212-3214 VB denotes be
T587 3215-3222 IN denotes between
T588 3234-3236 CD denotes 80
T589 3223-3224 CD denotes 1
T590 3225-3227 IN denotes in
T591 3228-3230 CD denotes 50
T592 3231-3233 IN denotes to
T593 3237-3241 IN denotes with
T594 3242-3244 DT denotes an
T595 3245-3254 NN denotes incidence
T596 3255-3257 IN denotes of
T597 3258-3266 JJ denotes clinical
T598 3267-3274 NN denotes disease
T599 3275-3283 VBN denotes expected
T600 3284-3286 IN denotes at
T601 3287-3293 IN denotes around
T602 3294-3295 CD denotes 1
T603 3299-3305 CD denotes 15,000
T604 3296-3298 IN denotes in
T605 3306-3307 -LRB- denotes [
T606 3311-3313 CD denotes 12
T607 3307-3308 CD denotes 1
T608 3308-3309 , denotes ,
T609 3309-3310 CD denotes 9
T610 3310-3311 , denotes ,
T611 3313-3314 -RRB- denotes ]
T612 3314-3315 . denotes .
T613 3315-3396 sentence denotes MCAD-deficient patients exhibit clinical episodes often associated with fasting.
T614 3316-3320 NN denotes MCAD
T615 3321-3330 JJ denotes deficient
T616 3320-3321 HYPH denotes -
T617 3331-3339 NNS denotes patients
T618 3340-3347 VBP denotes exhibit
T619 3348-3356 JJ denotes clinical
T620 3357-3365 NNS denotes episodes
T621 3366-3371 RB denotes often
T622 3372-3382 VBN denotes associated
T623 3383-3387 IN denotes with
T624 3388-3395 NN denotes fasting
T625 3395-3396 . denotes .
T626 3396-3466 sentence denotes Patients manifest disease usually during the first two years of life.
T627 3397-3405 NNS denotes Patients
T628 3406-3414 VBP denotes manifest
T629 3415-3422 NN denotes disease
T630 3423-3430 RB denotes usually
T631 3431-3437 IN denotes during
T632 3438-3441 DT denotes the
T633 3452-3457 NNS denotes years
T634 3442-3447 JJ denotes first
T635 3448-3451 CD denotes two
T636 3458-3460 IN denotes of
T637 3461-3465 NN denotes life
T638 3465-3466 . denotes .
T639 3466-3536 sentence denotes Symptoms include hypoketotic hypoglycemia and Reye-like episodes [1].
T640 3467-3475 NNS denotes Symptoms
T641 3476-3483 VBP denotes include
T642 3484-3495 JJ denotes hypoketotic
T643 3496-3508 NN denotes hypoglycemia
T644 3509-3512 CC denotes and
T645 3513-3517 NN denotes Reye
T646 3518-3522 JJ denotes like
T647 3517-3518 HYPH denotes -
T648 3523-3531 NNS denotes episodes
T649 3532-3533 -LRB- denotes [
T650 3533-3534 CD denotes 1
T651 3534-3535 -RRB- denotes ]
T652 3535-3536 . denotes .
T653 3536-3681 sentence denotes It is estimated that approximately 59% of patients presenting clinically between 15 to 26 mo of age die during their first clinical episode [1].
T654 3537-3539 PRP denotes It
T655 3543-3552 VBN denotes estimated
T656 3540-3542 VBZ denotes is
T657 3553-3557 IN denotes that
T658 3637-3640 VBP denotes die
T659 3558-3571 RB denotes approximately
T660 3572-3574 CD denotes 59
T661 3574-3575 NN denotes %
T662 3576-3578 IN denotes of
T663 3579-3587 NNS denotes patients
T664 3588-3598 VBG denotes presenting
T665 3599-3609 RB denotes clinically
T666 3610-3617 IN denotes between
T667 3618-3620 CD denotes 15
T668 3624-3626 CD denotes 26
T669 3621-3623 IN denotes to
T670 3627-3629 NN denotes mo
T671 3630-3632 IN denotes of
T672 3633-3636 NN denotes age
T673 3641-3647 IN denotes during
T674 3648-3653 PRP$ denotes their
T675 3669-3676 NN denotes episode
T676 3654-3659 JJ denotes first
T677 3660-3668 JJ denotes clinical
T678 3677-3678 -LRB- denotes [
T679 3678-3679 CD denotes 1
T680 3679-3680 -RRB- denotes ]
T681 3680-3681 . denotes .
T682 3681-3843 sentence denotes The pathogenesis of the wide range of metabolic disturbances in MCAD deficiency is poorly understood and certain aspects of patient management are controversial.
T683 3682-3685 DT denotes The
T684 3686-3698 NN denotes pathogenesis
T685 3772-3782 VBN denotes understood
T686 3699-3701 IN denotes of
T687 3702-3705 DT denotes the
T688 3711-3716 NN denotes range
T689 3706-3710 JJ denotes wide
T690 3717-3719 IN denotes of
T691 3720-3729 JJ denotes metabolic
T692 3730-3742 NNS denotes disturbances
T693 3743-3745 IN denotes in
T694 3746-3750 NN denotes MCAD
T695 3751-3761 NN denotes deficiency
T696 3762-3764 VBZ denotes is
T697 3765-3771 RB denotes poorly
T698 3783-3786 CC denotes and
T699 3787-3794 JJ denotes certain
T700 3795-3802 NNS denotes aspects
T701 3825-3828 VBP denotes are
T702 3803-3805 IN denotes of
T703 3806-3813 NN denotes patient
T704 3814-3824 NN denotes management
T705 3829-3842 JJ denotes controversial
T706 3842-3843 . denotes .
T707 3843-4011 sentence denotes An animal model for MCAD deficiency is essential to better understand the pathogenesis of MCAD deficiency and to develop better management regimens for human patients.
T708 3844-3846 DT denotes An
T709 3854-3859 NN denotes model
T710 3847-3853 NN denotes animal
T711 3880-3882 VBZ denotes is
T712 3860-3863 IN denotes for
T713 3864-3868 NN denotes MCAD
T714 3869-3879 NN denotes deficiency
T715 3883-3892 JJ denotes essential
T716 3893-3895 TO denotes to
T717 3903-3913 VB denotes understand
T718 3896-3902 RBR denotes better
T719 3914-3917 DT denotes the
T720 3918-3930 NN denotes pathogenesis
T721 3931-3933 IN denotes of
T722 3934-3938 NN denotes MCAD
T723 3939-3949 NN denotes deficiency
T724 3950-3953 CC denotes and
T725 3954-3956 TO denotes to
T726 3957-3964 VB denotes develop
T727 3965-3971 JJR denotes better
T728 3983-3991 NNS denotes regimens
T729 3972-3982 NN denotes management
T730 3992-3995 IN denotes for
T731 3996-4001 JJ denotes human
T732 4002-4010 NNS denotes patients
T733 4010-4011 . denotes .
T734 4011-4188 sentence denotes To gain further insight into the mechanisms of this disease, we developed a mouse model of MCAD deficiency by gene targeting in embryonic stem (ES) cells (for reviews [13,14]).
T735 4012-4014 TO denotes To
T736 4015-4019 VB denotes gain
T737 4076-4085 VBD denotes developed
T738 4020-4027 JJ denotes further
T739 4028-4035 NN denotes insight
T740 4036-4040 IN denotes into
T741 4041-4044 DT denotes the
T742 4045-4055 NNS denotes mechanisms
T743 4056-4058 IN denotes of
T744 4059-4063 DT denotes this
T745 4064-4071 NN denotes disease
T746 4071-4073 , denotes ,
T747 4073-4075 PRP denotes we
T748 4086-4087 DT denotes a
T749 4094-4099 NN denotes model
T750 4088-4093 NN denotes mouse
T751 4100-4102 IN denotes of
T752 4103-4107 NN denotes MCAD
T753 4108-4118 NN denotes deficiency
T754 4119-4121 IN denotes by
T755 4122-4126 NN denotes gene
T756 4127-4136 NN denotes targeting
T757 4137-4139 IN denotes in
T758 4140-4149 JJ denotes embryonic
T759 4150-4154 NN denotes stem
T760 4160-4165 NNS denotes cells
T761 4155-4156 -LRB- denotes (
T762 4156-4158 NN denotes ES
T763 4158-4159 -RRB- denotes )
T764 4166-4167 -LRB- denotes (
T765 4183-4185 CD denotes 14
T766 4167-4170 IN denotes for
T767 4171-4178 NNS denotes reviews
T768 4179-4180 -LRB- denotes [
T769 4180-4182 CD denotes 13
T770 4182-4183 , denotes ,
T771 4185-4186 -RRB- denotes ]
T772 4186-4187 -RRB- denotes )
T773 4187-4188 . denotes .
T774 4188-4338 sentence denotes The mutant mice had many relevant features characteristic of the disease found in human MCAD-deficient patients, along with some unexpected findings.
T775 4189-4192 DT denotes The
T776 4200-4204 NNS denotes mice
T777 4193-4199 NN denotes mutant
T778 4205-4208 VBD denotes had
T779 4209-4213 JJ denotes many
T780 4223-4231 NNS denotes features
T781 4214-4222 JJ denotes relevant
T782 4232-4246 JJ denotes characteristic
T783 4247-4249 IN denotes of
T784 4250-4253 DT denotes the
T785 4254-4261 NN denotes disease
T786 4262-4267 VBN denotes found
T787 4268-4270 IN denotes in
T788 4271-4276 JJ denotes human
T789 4292-4300 NNS denotes patients
T790 4277-4281 NN denotes MCAD
T791 4282-4291 JJ denotes deficient
T792 4281-4282 HYPH denotes -
T793 4300-4302 , denotes ,
T794 4302-4307 IN denotes along
T795 4308-4312 IN denotes with
T796 4313-4317 DT denotes some
T797 4329-4337 NNS denotes findings
T798 4318-4328 JJ denotes unexpected
T799 4337-4338 . denotes .
T964 4349-4353 NN denotes Gene
T965 4354-4363 NN denotes Targeting
T966 4364-4367 CC denotes and
T967 4368-4378 NN denotes Generation
T968 4379-4381 IN denotes of
T969 4382-4386 NN denotes MCAD
T970 4387-4396 JJ denotes Deficient
T971 4386-4387 HYPH denotes -
T972 4397-4401 NNS denotes Mice
T973 4401-4568 sentence denotes MCAD insertion vector (MCAD IV2) was designed to undergo gap repair of the 1.3-kb deleted region upon homologous recombination in 129P2 (129P2/OlaHsd) ES cells E14–1.
T974 4402-4406 NN denotes MCAD
T975 4417-4423 NN denotes vector
T976 4407-4416 NN denotes insertion
T977 4439-4447 VBN denotes designed
T978 4424-4425 -LRB- denotes (
T979 4425-4429 NN denotes MCAD
T980 4430-4433 NN denotes IV2
T981 4433-4434 -RRB- denotes )
T982 4435-4438 VBD denotes was
T983 4448-4450 TO denotes to
T984 4451-4458 VB denotes undergo
T985 4459-4462 NN denotes gap
T986 4463-4469 NN denotes repair
T987 4470-4472 IN denotes of
T988 4473-4476 DT denotes the
T989 4492-4498 NN denotes region
T990 4477-4480 CD denotes 1.3
T991 4481-4483 NN denotes kb
T992 4480-4481 HYPH denotes -
T993 4484-4491 VBN denotes deleted
T994 4499-4503 IN denotes upon
T995 4504-4514 JJ denotes homologous
T996 4515-4528 NN denotes recombination
T997 4529-4531 IN denotes in
T998 4532-4537 NN denotes 129P2
T999 4556-4561 NNS denotes cells
T1000 4538-4539 -LRB- denotes (
T1001 4545-4551 NN denotes OlaHsd
T1002 4539-4544 NN denotes 129P2
T1003 4544-4545 HYPH denotes /
T1004 4551-4552 -RRB- denotes )
T1005 4553-4555 NN denotes ES
T1006 4562-4565 NN denotes E14
T1007 4565-4566 HYPH denotes –
T1008 4566-4567 CD denotes 1
T1009 4567-4568 . denotes .
T1010 4568-4719 sentence denotes Correct targeting of the MCAD locus resulted in a duplication of exons 8, 9, and 10 and integration of flanking plasmid and Neo sequences (Figure 1A).
T1011 4569-4576 JJ denotes Correct
T1012 4577-4586 NN denotes targeting
T1013 4605-4613 VBD denotes resulted
T1014 4587-4589 IN denotes of
T1015 4590-4593 DT denotes the
T1016 4599-4604 NN denotes locus
T1017 4594-4598 NN denotes MCAD
T1018 4614-4616 IN denotes in
T1019 4617-4618 DT denotes a
T1020 4619-4630 NN denotes duplication
T1021 4631-4633 IN denotes of
T1022 4634-4639 NNS denotes exons
T1023 4640-4641 CD denotes 8
T1024 4641-4643 , denotes ,
T1025 4643-4644 CD denotes 9
T1026 4644-4646 , denotes ,
T1027 4646-4649 CC denotes and
T1028 4650-4652 CD denotes 10
T1029 4653-4656 CC denotes and
T1030 4657-4668 NN denotes integration
T1031 4669-4671 IN denotes of
T1032 4672-4680 VBG denotes flanking
T1033 4681-4688 NN denotes plasmid
T1034 4689-4692 CC denotes and
T1035 4693-4696 NN denotes Neo
T1036 4697-4706 NNS denotes sequences
T1037 4707-4708 -LRB- denotes (
T1038 4708-4714 NN denotes Figure
T1039 4715-4717 CD denotes 1A
T1040 4717-4718 -RRB- denotes )
T1041 4718-4719 . denotes .
T1042 4719-4803 sentence denotes The insertion vector was designed to duplicate exon 8, 9, and 10 at the MCAD locus.
T1043 4720-4723 DT denotes The
T1044 4734-4740 NN denotes vector
T1045 4724-4733 NN denotes insertion
T1046 4745-4753 VBN denotes designed
T1047 4741-4744 VBD denotes was
T1048 4754-4756 TO denotes to
T1049 4757-4766 VB denotes duplicate
T1050 4767-4771 NN denotes exon
T1051 4772-4773 CD denotes 8
T1052 4773-4775 , denotes ,
T1053 4775-4776 CD denotes 9
T1054 4776-4778 , denotes ,
T1055 4778-4781 CC denotes and
T1056 4782-4784 CD denotes 10
T1057 4785-4787 IN denotes at
T1058 4788-4791 DT denotes the
T1059 4797-4802 NN denotes locus
T1060 4792-4796 NN denotes MCAD
T1061 4802-4803 . denotes .
T1062 4803-4942 sentence denotes Translation of the duplicated exon 8 region results in the formation of premature stop codons resulting in truncation of the MCAD monomer.
T1063 4804-4815 NN denotes Translation
T1064 4848-4855 VBZ denotes results
T1065 4816-4818 IN denotes of
T1066 4819-4822 DT denotes the
T1067 4841-4847 NN denotes region
T1068 4823-4833 VBN denotes duplicated
T1069 4834-4838 NN denotes exon
T1070 4839-4840 CD denotes 8
T1071 4856-4858 IN denotes in
T1072 4859-4862 DT denotes the
T1073 4863-4872 NN denotes formation
T1074 4873-4875 IN denotes of
T1075 4876-4885 JJ denotes premature
T1076 4891-4897 NNS denotes codons
T1077 4886-4890 NN denotes stop
T1078 4898-4907 VBG denotes resulting
T1079 4908-4910 IN denotes in
T1080 4911-4921 NN denotes truncation
T1081 4922-4924 IN denotes of
T1082 4925-4928 DT denotes the
T1083 4934-4941 NN denotes monomer
T1084 4929-4933 NN denotes MCAD
T1085 4941-4942 . denotes .
T1086 4942-5066 sentence denotes Specifically, the first premature stop codon arises after translation of only seven amino acids from the duplicated exon 8.
T1087 4943-4955 RB denotes Specifically
T1088 4988-4994 VBZ denotes arises
T1089 4955-4957 , denotes ,
T1090 4957-4960 DT denotes the
T1091 4982-4987 NN denotes codon
T1092 4961-4966 JJ denotes first
T1093 4967-4976 JJ denotes premature
T1094 4977-4981 NN denotes stop
T1095 4995-5000 IN denotes after
T1096 5001-5012 NN denotes translation
T1097 5013-5015 IN denotes of
T1098 5016-5020 RB denotes only
T1099 5021-5026 CD denotes seven
T1100 5033-5038 NNS denotes acids
T1101 5027-5032 NN denotes amino
T1102 5039-5043 IN denotes from
T1103 5044-5047 DT denotes the
T1104 5059-5063 NN denotes exon
T1105 5048-5058 VBN denotes duplicated
T1106 5064-5065 CD denotes 8
T1107 5065-5066 . denotes .
T1108 5066-5236 sentence denotes The resulting MCAD monomer is missing the C-terminal domain α-helixes that are responsible for making intersubunit contacts to generate the functional MCAD homotetramer.
T1109 5067-5070 DT denotes The
T1110 5086-5093 NN denotes monomer
T1111 5071-5080 VBG denotes resulting
T1112 5081-5085 NN denotes MCAD
T1113 5097-5104 VBG denotes missing
T1114 5094-5096 VBZ denotes is
T1115 5105-5108 DT denotes the
T1116 5129-5136 NNS denotes helixes
T1117 5109-5110 NN denotes C
T1118 5111-5119 JJ denotes terminal
T1119 5110-5111 HYPH denotes -
T1120 5120-5126 NN denotes domain
T1121 5127-5128 NN denotes α
T1122 5128-5129 HYPH denotes -
T1123 5137-5141 WDT denotes that
T1124 5142-5145 VBP denotes are
T1125 5146-5157 JJ denotes responsible
T1126 5158-5161 IN denotes for
T1127 5162-5168 VBG denotes making
T1128 5169-5181 NN denotes intersubunit
T1129 5182-5190 NNS denotes contacts
T1130 5191-5193 TO denotes to
T1131 5194-5202 VB denotes generate
T1132 5203-5206 DT denotes the
T1133 5223-5235 NN denotes homotetramer
T1134 5207-5217 JJ denotes functional
T1135 5218-5222 NN denotes MCAD
T1136 5235-5236 . denotes .
T1137 5236-6060 sentence denotes Figure 1 Strategy for Disruption of the Mouse Acadm Gene (A) The MCAD IV2 insertion targeting vector with a deleted 1.3-kb region encompassing exon 10 and flanking sequences. MCAD IV2 undergoes gap repair upon homologous recombination at the endogenous Acadm locus resulting in a duplication of exons 8, 9, and 10 at the disrupted allele. (B) Southern blot analysis of EcoRI-digested genomic DNA from ES cells screened by PCR. Probe A, a DNA fragment consisting of a portion of exon 10 that is not present in the targeting vector, hybridizes to an endogenous 3.1-kb fragment and, upon homologous recombination, to a 13.2-kb fragment. Lane 1 represents a wild-type ES cell line, and Lane 2 and 3 represent targeted ES cell lines. ES cell clones were screened by PCR (data not shown) and confirmed by Southern blot analysis.
T1138 5967-5969 NN denotes ES
T1139 5970-5974 NN denotes cell
T1140 5975-5981 NNS denotes clones
T1141 5987-5995 VBN denotes screened
T1142 5982-5986 VBD denotes were
T1143 5996-5998 IN denotes by
T1144 5999-6002 NN denotes PCR
T1145 6003-6004 -LRB- denotes (
T1146 6013-6018 VBN denotes shown
T1147 6004-6008 NNS denotes data
T1148 6009-6012 RB denotes not
T1149 6018-6019 -RRB- denotes )
T1150 6020-6023 CC denotes and
T1151 6024-6033 VBN denotes confirmed
T1152 6034-6036 IN denotes by
T1153 6037-6045 NNP denotes Southern
T1154 6046-6050 NN denotes blot
T1155 6051-6059 NN denotes analysis
T1156 6059-6060 . denotes .
T1157 6060-6300 sentence denotes Southern blot analysis used an exon 10 probe (probe A), not present in the targeting vector, hybridized to a 13.2-kb band in addition to the 3.1-kb endogenous band indicating targeted insertion of the vector at the Acadm locus (Figure 1B).
T1158 6061-6069 NNP denotes Southern
T1159 6070-6074 NN denotes blot
T1160 6075-6083 NN denotes analysis
T1161 6084-6088 VBD denotes used
T1162 6089-6091 DT denotes an
T1163 6100-6105 NN denotes probe
T1164 6092-6096 NN denotes exon
T1165 6097-6099 CD denotes 10
T1166 6106-6107 -LRB- denotes (
T1167 6113-6114 NN denotes A
T1168 6107-6112 NN denotes probe
T1169 6114-6115 -RRB- denotes )
T1170 6115-6117 , denotes ,
T1171 6117-6120 RB denotes not
T1172 6121-6128 JJ denotes present
T1173 6129-6131 IN denotes in
T1174 6132-6135 DT denotes the
T1175 6146-6152 NN denotes vector
T1176 6136-6145 NN denotes targeting
T1177 6152-6154 , denotes ,
T1178 6154-6164 VBN denotes hybridized
T1179 6165-6167 IN denotes to
T1180 6168-6169 DT denotes a
T1181 6178-6182 NN denotes band
T1182 6170-6174 CD denotes 13.2
T1183 6175-6177 NN denotes kb
T1184 6174-6175 HYPH denotes -
T1185 6183-6185 IN denotes in
T1186 6186-6194 NN denotes addition
T1187 6195-6197 IN denotes to
T1188 6198-6201 DT denotes the
T1189 6220-6224 NN denotes band
T1190 6202-6205 CD denotes 3.1
T1191 6206-6208 NN denotes kb
T1192 6205-6206 HYPH denotes -
T1193 6209-6219 JJ denotes endogenous
T1194 6225-6235 VBG denotes indicating
T1195 6236-6244 VBN denotes targeted
T1196 6245-6254 NN denotes insertion
T1197 6255-6257 IN denotes of
T1198 6258-6261 DT denotes the
T1199 6262-6268 NN denotes vector
T1200 6269-6271 IN denotes at
T1201 6272-6275 DT denotes the
T1202 6282-6287 NN denotes locus
T1203 6276-6281 NN denotes Acadm
T1204 6288-6289 -LRB- denotes (
T1205 6289-6295 NN denotes Figure
T1206 6296-6298 CD denotes 1B
T1207 6298-6299 -RRB- denotes )
T1208 6299-6300 . denotes .
T1209 6300-6414 sentence denotes Correctly targeted ES cell clones were microinjected into B6 (C57BL/6NTac) blastocysts to generate chimeric mice.
T1210 6301-6310 RB denotes Correctly
T1211 6311-6319 VBN denotes targeted
T1212 6328-6334 NNS denotes clones
T1213 6320-6322 NN denotes ES
T1214 6323-6327 NN denotes cell
T1215 6340-6353 VBN denotes microinjected
T1216 6335-6339 VBD denotes were
T1217 6354-6358 IN denotes into
T1218 6359-6361 NN denotes B6
T1219 6376-6387 NNS denotes blastocysts
T1220 6362-6363 -LRB- denotes (
T1221 6363-6368 NN denotes C57BL
T1222 6369-6374 NN denotes 6NTac
T1223 6368-6369 HYPH denotes /
T1224 6374-6375 -RRB- denotes )
T1225 6388-6390 TO denotes to
T1226 6391-6399 VB denotes generate
T1227 6400-6408 JJ denotes chimeric
T1228 6409-6413 NNS denotes mice
T1229 6413-6414 . denotes .
T1230 6414-6555 sentence denotes Chimeric mice were backcrossed to both 129P2 and B6 inbred mice to produce MCAD+/− and eventually MCAD−/− mice on a B6/129 mixed background.
T1231 6415-6423 JJ denotes Chimeric
T1232 6424-6428 NNS denotes mice
T1233 6434-6445 VBN denotes backcrossed
T1234 6429-6433 VBD denotes were
T1235 6446-6448 IN denotes to
T1236 6449-6453 CC denotes both
T1237 6454-6459 NN denotes 129P2
T1238 6467-6473 VBN denotes inbred
T1239 6460-6463 CC denotes and
T1240 6464-6466 NN denotes B6
T1241 6474-6478 NNS denotes mice
T1242 6479-6481 TO denotes to
T1243 6482-6489 VB denotes produce
T1244 6490-6494 NN denotes MCAD
T1245 6521-6525 NNS denotes mice
T1246 6494-6495 SYM denotes +
T1247 6495-6496 HYPH denotes /
T1248 6496-6497 SYM denotes −
T1249 6498-6501 CC denotes and
T1250 6502-6512 RB denotes eventually
T1251 6513-6517 NN denotes MCAD
T1252 6517-6518 SYM denotes −
T1253 6518-6519 HYPH denotes /
T1254 6519-6520 SYM denotes −
T1255 6526-6528 IN denotes on
T1256 6529-6530 DT denotes a
T1257 6544-6554 NN denotes background
T1258 6531-6533 NN denotes B6
T1259 6533-6534 HYPH denotes /
T1260 6534-6537 CD denotes 129
T1261 6538-6543 JJ denotes mixed
T1262 6554-6555 . denotes .
T1263 6555-6747 sentence denotes The studies described here were conducted exclusively on the B6/129 mixed background compared with littermate controls or B6/129 control groups maintained by intercrosses as were the mutants.
T1264 6556-6559 DT denotes The
T1265 6560-6567 NNS denotes studies
T1266 6588-6597 VBN denotes conducted
T1267 6568-6577 VBN denotes described
T1268 6578-6582 RB denotes here
T1269 6583-6587 VBD denotes were
T1270 6598-6609 RB denotes exclusively
T1271 6610-6612 IN denotes on
T1272 6613-6616 DT denotes the
T1273 6630-6640 NN denotes background
T1274 6617-6619 NN denotes B6
T1275 6619-6620 HYPH denotes /
T1276 6620-6623 CD denotes 129
T1277 6624-6629 JJ denotes mixed
T1278 6641-6649 VBN denotes compared
T1279 6650-6654 IN denotes with
T1280 6655-6665 NN denotes littermate
T1281 6666-6674 NNS denotes controls
T1282 6675-6677 CC denotes or
T1283 6678-6680 NN denotes B6
T1284 6693-6699 NNS denotes groups
T1285 6680-6681 HYPH denotes /
T1286 6681-6684 CD denotes 129
T1287 6685-6692 NN denotes control
T1288 6700-6710 VBN denotes maintained
T1289 6711-6713 IN denotes by
T1290 6714-6726 NNS denotes intercrosses
T1291 6727-6729 IN denotes as
T1292 6730-6734 VBD denotes were
T1293 6735-6738 DT denotes the
T1294 6739-6746 NNS denotes mutants
T1295 6746-6747 . denotes .
T1296 6747-6844 sentence denotes Perpetuating this mutation as a congenic mutant line on the 129P2 background proved impractical.
T1297 6748-6760 VBG denotes Perpetuating
T1298 6825-6831 VBD denotes proved
T1299 6761-6765 DT denotes this
T1300 6766-6774 NN denotes mutation
T1301 6775-6777 IN denotes as
T1302 6778-6779 DT denotes a
T1303 6796-6800 NN denotes line
T1304 6780-6788 JJ denotes congenic
T1305 6789-6795 NN denotes mutant
T1306 6801-6803 IN denotes on
T1307 6804-6807 DT denotes the
T1308 6814-6824 NN denotes background
T1309 6808-6813 NN denotes 129P2
T1310 6832-6843 JJ denotes impractical
T1311 6843-6844 . denotes .
T1312 6844-7012 sentence denotes The 129P2 mice were poor breeders as wild-types, and when introduced, the Acadm mutation was nearly lost on this background because of the high rate of neonatal death.
T1313 6845-6848 DT denotes The
T1314 6855-6859 NNS denotes mice
T1315 6849-6854 NN denotes 129P2
T1316 6860-6864 VBD denotes were
T1317 6865-6869 JJ denotes poor
T1318 6870-6878 NNS denotes breeders
T1319 6879-6881 IN denotes as
T1320 6882-6886 JJ denotes wild
T1321 6887-6892 NNS denotes types
T1322 6886-6887 HYPH denotes -
T1323 6892-6894 , denotes ,
T1324 6894-6897 CC denotes and
T1325 6898-6902 WRB denotes when
T1326 6903-6913 VBN denotes introduced
T1327 6945-6949 VBN denotes lost
T1328 6913-6915 , denotes ,
T1329 6915-6918 DT denotes the
T1330 6925-6933 NN denotes mutation
T1331 6919-6924 NN denotes Acadm
T1332 6934-6937 VBD denotes was
T1333 6938-6944 RB denotes nearly
T1334 6950-6952 IN denotes on
T1335 6953-6957 DT denotes this
T1336 6958-6968 NN denotes background
T1337 6969-6976 IN denotes because
T1338 6977-6979 IN denotes of
T1339 6980-6983 DT denotes the
T1340 6989-6993 NN denotes rate
T1341 6984-6988 JJ denotes high
T1342 6994-6996 IN denotes of
T1343 6997-7005 JJ denotes neonatal
T1344 7006-7011 NN denotes death
T1345 7011-7012 . denotes .
T1346 7012-7148 sentence denotes Because of the molecular structure of the targeted allele, it proved virtually impossible to distinguish all three potential genotypes.
T1347 7013-7020 IN denotes Because
T1348 7075-7081 VBD denotes proved
T1349 7021-7023 IN denotes of
T1350 7024-7027 DT denotes the
T1351 7038-7047 NN denotes structure
T1352 7028-7037 JJ denotes molecular
T1353 7048-7050 IN denotes of
T1354 7051-7054 DT denotes the
T1355 7064-7070 NN denotes allele
T1356 7055-7063 VBN denotes targeted
T1357 7070-7072 , denotes ,
T1358 7072-7074 PRP denotes it
T1359 7082-7091 RB denotes virtually
T1360 7092-7102 JJ denotes impossible
T1361 7103-7105 TO denotes to
T1362 7106-7117 VB denotes distinguish
T1363 7118-7121 DT denotes all
T1364 7138-7147 NNS denotes genotypes
T1365 7122-7127 CD denotes three
T1366 7128-7137 JJ denotes potential
T1367 7147-7148 . denotes .
T1368 7148-7343 sentence denotes We could clearly detect the presence or absence of the targeted allele, however, whether a particular mouse was MCAD−/− or MCAD+/− could not be determined by Southern blot or PCR of genomic DNA.
T1369 7149-7151 PRP denotes We
T1370 7166-7172 VB denotes detect
T1371 7152-7157 MD denotes could
T1372 7158-7165 RB denotes clearly
T1373 7293-7303 VBN denotes determined
T1374 7173-7176 DT denotes the
T1375 7177-7185 NN denotes presence
T1376 7186-7188 CC denotes or
T1377 7189-7196 NN denotes absence
T1378 7197-7199 IN denotes of
T1379 7200-7203 DT denotes the
T1380 7213-7219 NN denotes allele
T1381 7204-7212 VBN denotes targeted
T1382 7219-7221 , denotes ,
T1383 7221-7228 RB denotes however
T1384 7228-7230 , denotes ,
T1385 7230-7237 IN denotes whether
T1386 7257-7260 VBD denotes was
T1387 7238-7239 DT denotes a
T1388 7251-7256 NN denotes mouse
T1389 7240-7250 JJ denotes particular
T1390 7261-7265 NN denotes MCAD
T1391 7265-7266 SYM denotes −
T1392 7266-7267 HYPH denotes /
T1393 7267-7268 SYM denotes −
T1394 7269-7271 CC denotes or
T1395 7272-7276 NN denotes MCAD
T1396 7276-7277 SYM denotes +
T1397 7277-7278 HYPH denotes /
T1398 7278-7279 SYM denotes −
T1399 7280-7285 MD denotes could
T1400 7286-7289 RB denotes not
T1401 7290-7292 VB denotes be
T1402 7304-7306 IN denotes by
T1403 7307-7315 NNP denotes Southern
T1404 7316-7320 NN denotes blot
T1405 7321-7323 CC denotes or
T1406 7324-7327 NN denotes PCR
T1407 7328-7330 IN denotes of
T1408 7331-7338 JJ denotes genomic
T1409 7339-7342 NN denotes DNA
T1410 7342-7343 . denotes .
T1411 7343-7497 sentence denotes Ultimately MCAD−/− mice were ascertained by immunoblot analysis of offspring with subsequent perpetuation of MCAD−/− and MCAD+/+ mice as separate groups.
T1412 7344-7354 RB denotes Ultimately
T1413 7373-7384 VBN denotes ascertained
T1414 7355-7359 NN denotes MCAD
T1415 7363-7367 NNS denotes mice
T1416 7359-7360 SYM denotes −
T1417 7360-7361 HYPH denotes /
T1418 7361-7362 SYM denotes −
T1419 7368-7372 VBD denotes were
T1420 7385-7387 IN denotes by
T1421 7388-7398 NN denotes immunoblot
T1422 7399-7407 NN denotes analysis
T1423 7408-7410 IN denotes of
T1424 7411-7420 NN denotes offspring
T1425 7421-7425 IN denotes with
T1426 7426-7436 JJ denotes subsequent
T1427 7437-7449 NN denotes perpetuation
T1428 7450-7452 IN denotes of
T1429 7453-7457 NN denotes MCAD
T1430 7473-7477 NNS denotes mice
T1431 7457-7458 SYM denotes −
T1432 7458-7459 HYPH denotes /
T1433 7459-7460 SYM denotes −
T1434 7461-7464 CC denotes and
T1435 7465-7469 NN denotes MCAD
T1436 7469-7470 SYM denotes +
T1437 7470-7471 HYPH denotes /
T1438 7471-7472 SYM denotes +
T1439 7478-7480 IN denotes as
T1440 7481-7489 JJ denotes separate
T1441 7490-7496 NNS denotes groups
T1442 7496-7497 . denotes .
T1606 7499-7502 NN denotes RNA
T1607 7503-7511 NN denotes Analysis
T1608 7511-7707 sentence denotes RT-PCR amplification from exon 7 to 11 from total heart RNA amplified the expected 600-base pair (bp) fragment in MCAD+/+ and MCAD+/− mice, and a 1.5-kb fragment in MCAD−/− mice (data not shown).
T1609 7512-7514 NN denotes RT
T1610 7515-7518 NN denotes PCR
T1611 7514-7515 HYPH denotes -
T1612 7519-7532 NN denotes amplification
T1613 7572-7581 VBD denotes amplified
T1614 7533-7537 IN denotes from
T1615 7538-7542 NN denotes exon
T1616 7543-7544 CD denotes 7
T1617 7545-7547 IN denotes to
T1618 7548-7550 CD denotes 11
T1619 7551-7555 IN denotes from
T1620 7556-7561 JJ denotes total
T1621 7568-7571 NN denotes RNA
T1622 7562-7567 NN denotes heart
T1623 7582-7585 DT denotes the
T1624 7614-7622 NN denotes fragment
T1625 7586-7594 VBN denotes expected
T1626 7595-7598 CD denotes 600
T1627 7604-7608 NN denotes pair
T1628 7598-7599 HYPH denotes -
T1629 7599-7603 NN denotes base
T1630 7609-7610 -LRB- denotes (
T1631 7610-7612 NN denotes bp
T1632 7612-7613 -RRB- denotes )
T1633 7623-7625 IN denotes in
T1634 7626-7630 NN denotes MCAD
T1635 7646-7650 NNS denotes mice
T1636 7630-7631 SYM denotes +
T1637 7631-7632 HYPH denotes /
T1638 7632-7633 SYM denotes +
T1639 7634-7637 CC denotes and
T1640 7638-7642 NN denotes MCAD
T1641 7642-7643 SYM denotes +
T1642 7643-7644 HYPH denotes /
T1643 7644-7645 SYM denotes −
T1644 7650-7652 , denotes ,
T1645 7652-7655 CC denotes and
T1646 7656-7657 DT denotes a
T1647 7665-7673 NN denotes fragment
T1648 7658-7661 CD denotes 1.5
T1649 7662-7664 NN denotes kb
T1650 7661-7662 HYPH denotes -
T1651 7674-7676 IN denotes in
T1652 7677-7681 NN denotes MCAD
T1653 7685-7689 NNS denotes mice
T1654 7681-7682 SYM denotes −
T1655 7682-7683 HYPH denotes /
T1656 7683-7684 SYM denotes −
T1657 7690-7691 -LRB- denotes (
T1658 7700-7705 VBN denotes shown
T1659 7691-7695 NNS denotes data
T1660 7696-7699 RB denotes not
T1661 7705-7706 -RRB- denotes )
T1662 7706-7707 . denotes .
T1663 7707-7877 sentence denotes Sequence analysis of the 1.5-kb fragment revealed that the amplified fragment consisted of exon 7 to exon 10 with 280 bp of pGEM plasmid sequence followed by exons 8–11.
T1664 7708-7716 NN denotes Sequence
T1665 7717-7725 NN denotes analysis
T1666 7749-7757 VBD denotes revealed
T1667 7726-7728 IN denotes of
T1668 7729-7732 DT denotes the
T1669 7740-7748 NN denotes fragment
T1670 7733-7736 CD denotes 1.5
T1671 7737-7739 NN denotes kb
T1672 7736-7737 HYPH denotes -
T1673 7758-7762 IN denotes that
T1674 7786-7795 VBD denotes consisted
T1675 7763-7766 DT denotes the
T1676 7777-7785 NN denotes fragment
T1677 7767-7776 VBN denotes amplified
T1678 7796-7798 IN denotes of
T1679 7799-7803 NN denotes exon
T1680 7804-7805 CD denotes 7
T1681 7806-7808 IN denotes to
T1682 7809-7813 NN denotes exon
T1683 7814-7816 CD denotes 10
T1684 7817-7821 IN denotes with
T1685 7822-7825 CD denotes 280
T1686 7826-7828 NN denotes bp
T1687 7829-7831 IN denotes of
T1688 7832-7836 NN denotes pGEM
T1689 7845-7853 NN denotes sequence
T1690 7837-7844 NN denotes plasmid
T1691 7854-7862 VBN denotes followed
T1692 7863-7865 IN denotes by
T1693 7866-7871 NNS denotes exons
T1694 7872-7873 CD denotes 8
T1695 7873-7874 SYM denotes –
T1696 7874-7876 CD denotes 11
T1697 7876-7877 . denotes .
T1698 7877-7980 sentence denotes Some of the plasmid sequences, along with the pPGKNEOpA sequence, were deleted from this spliced mRNA.
T1699 7878-7882 DT denotes Some
T1700 7949-7956 VBN denotes deleted
T1701 7883-7885 IN denotes of
T1702 7886-7889 DT denotes the
T1703 7898-7907 NNS denotes sequences
T1704 7890-7897 NN denotes plasmid
T1705 7907-7909 , denotes ,
T1706 7909-7914 IN denotes along
T1707 7915-7919 IN denotes with
T1708 7920-7923 DT denotes the
T1709 7934-7942 NN denotes sequence
T1710 7924-7933 NN denotes pPGKNEOpA
T1711 7942-7944 , denotes ,
T1712 7944-7948 VBD denotes were
T1713 7957-7961 IN denotes from
T1714 7962-7966 DT denotes this
T1715 7975-7979 NN denotes mRNA
T1716 7967-7974 VBN denotes spliced
T1717 7979-7980 . denotes .
T1718 7980-8253 sentence denotes Northern blot analysis revealed Acadm was normally expressed in all tissues analyzed from MCAD+/+ mice with the most robust expression occurring in brown fat, kidney, heart, skeletal muscle, and liver with minimal expression in the brain, white fat, and testes (Figure 2).
T1719 7981-7989 NNP denotes Northern
T1720 7990-7994 NN denotes blot
T1721 7995-8003 NN denotes analysis
T1722 8004-8012 VBD denotes revealed
T1723 8013-8018 NN denotes Acadm
T1724 8032-8041 VBN denotes expressed
T1725 8019-8022 VBD denotes was
T1726 8023-8031 RB denotes normally
T1727 8042-8044 IN denotes in
T1728 8045-8048 DT denotes all
T1729 8049-8056 NNS denotes tissues
T1730 8057-8065 VBN denotes analyzed
T1731 8066-8070 IN denotes from
T1732 8071-8075 NN denotes MCAD
T1733 8079-8083 NNS denotes mice
T1734 8075-8076 SYM denotes +
T1735 8076-8077 HYPH denotes /
T1736 8077-8078 SYM denotes +
T1737 8084-8088 IN denotes with
T1738 8089-8092 DT denotes the
T1739 8105-8115 NN denotes expression
T1740 8093-8097 RBS denotes most
T1741 8098-8104 JJ denotes robust
T1742 8116-8125 VBG denotes occurring
T1743 8126-8128 IN denotes in
T1744 8129-8134 JJ denotes brown
T1745 8135-8138 NN denotes fat
T1746 8138-8140 , denotes ,
T1747 8140-8146 NN denotes kidney
T1748 8146-8148 , denotes ,
T1749 8148-8153 NN denotes heart
T1750 8153-8155 , denotes ,
T1751 8155-8163 JJ denotes skeletal
T1752 8164-8170 NN denotes muscle
T1753 8170-8172 , denotes ,
T1754 8172-8175 CC denotes and
T1755 8176-8181 NN denotes liver
T1756 8182-8186 IN denotes with
T1757 8187-8194 JJ denotes minimal
T1758 8195-8205 NN denotes expression
T1759 8206-8208 IN denotes in
T1760 8209-8212 DT denotes the
T1761 8213-8218 NN denotes brain
T1762 8218-8220 , denotes ,
T1763 8220-8225 JJ denotes white
T1764 8226-8229 NN denotes fat
T1765 8229-8231 , denotes ,
T1766 8231-8234 CC denotes and
T1767 8235-8241 NNS denotes testes
T1768 8242-8243 -LRB- denotes (
T1769 8243-8249 NN denotes Figure
T1770 8250-8251 CD denotes 2
T1771 8251-8252 -RRB- denotes )
T1772 8252-8253 . denotes .
T1773 8253-8415 sentence denotes Interestingly, although RT-PCR amplified an incorrectly spliced Acadm transcript, no Acadm transcripts were detected by northern blot analysis from MCAD−/− mice.
T1774 8254-8267 RB denotes Interestingly
T1775 8362-8370 VBN denotes detected
T1776 8267-8269 , denotes ,
T1777 8269-8277 IN denotes although
T1778 8285-8294 VBD denotes amplified
T1779 8278-8280 NN denotes RT
T1780 8281-8284 NN denotes PCR
T1781 8280-8281 HYPH denotes -
T1782 8295-8297 DT denotes an
T1783 8324-8334 NN denotes transcript
T1784 8298-8309 RB denotes incorrectly
T1785 8310-8317 VBN denotes spliced
T1786 8318-8323 NN denotes Acadm
T1787 8334-8336 , denotes ,
T1788 8336-8338 DT denotes no
T1789 8345-8356 NNS denotes transcripts
T1790 8339-8344 NN denotes Acadm
T1791 8357-8361 VBD denotes were
T1792 8371-8373 IN denotes by
T1793 8374-8382 NNP denotes northern
T1794 8383-8387 NN denotes blot
T1795 8388-8396 NN denotes analysis
T1796 8397-8401 IN denotes from
T1797 8402-8406 NN denotes MCAD
T1798 8410-8414 NNS denotes mice
T1799 8406-8407 SYM denotes −
T1800 8407-8408 HYPH denotes /
T1801 8408-8409 SYM denotes −
T1802 8414-8415 . denotes .
T1803 8415-8557 sentence denotes These results strongly suggest that the mutant RNA is unstable and degraded rapidly or, alternatively, undergoes nonsense mediated RNA decay.
T1804 8416-8421 DT denotes These
T1805 8422-8429 NNS denotes results
T1806 8439-8446 VBP denotes suggest
T1807 8430-8438 RB denotes strongly
T1808 8447-8451 IN denotes that
T1809 8467-8469 VBZ denotes is
T1810 8452-8455 DT denotes the
T1811 8463-8466 NN denotes RNA
T1812 8456-8462 NN denotes mutant
T1813 8470-8478 JJ denotes unstable
T1814 8479-8482 CC denotes and
T1815 8483-8491 VBN denotes degraded
T1816 8492-8499 RB denotes rapidly
T1817 8500-8502 CC denotes or
T1818 8502-8504 , denotes ,
T1819 8504-8517 RB denotes alternatively
T1820 8519-8528 VBZ denotes undergoes
T1821 8517-8519 , denotes ,
T1822 8529-8537 NN denotes nonsense
T1823 8538-8546 VBN denotes mediated
T1824 8551-8556 NN denotes decay
T1825 8547-8550 NN denotes RNA
T1826 8556-8557 . denotes .
T1979 8936-8941 NN denotes Liver
T1980 8942-8948 NN denotes Enzyme
T1981 8949-8957 NNS denotes Analyses
T1982 8957-9129 sentence denotes Immunoblot analyses of liver homogenates with anti-MCAD antisera demonstrated that the 42 kDa MCAD monomer was present in MCAD+/+ mice, but not in MCAD−/− mice (Figure 3).
T1983 8958-8968 NN denotes Immunoblot
T1984 8969-8977 NNS denotes analyses
T1985 9023-9035 VBD denotes demonstrated
T1986 8978-8980 IN denotes of
T1987 8981-8986 NN denotes liver
T1988 8987-8998 NNS denotes homogenates
T1989 8999-9003 IN denotes with
T1990 9004-9013 JJ denotes anti-MCAD
T1991 9014-9022 NNS denotes antisera
T1992 9036-9040 IN denotes that
T1993 9065-9068 VBD denotes was
T1994 9041-9044 DT denotes the
T1995 9057-9064 NN denotes monomer
T1996 9045-9047 CD denotes 42
T1997 9048-9051 NN denotes kDa
T1998 9052-9056 NN denotes MCAD
T1999 9069-9076 JJ denotes present
T2000 9077-9079 IN denotes in
T2001 9080-9084 NN denotes MCAD
T2002 9088-9092 NNS denotes mice
T2003 9084-9085 SYM denotes +
T2004 9085-9086 HYPH denotes /
T2005 9086-9087 SYM denotes +
T2006 9092-9094 , denotes ,
T2007 9094-9097 CC denotes but
T2008 9098-9101 RB denotes not
T2009 9102-9104 IN denotes in
T2010 9105-9109 NN denotes MCAD
T2011 9113-9117 NNS denotes mice
T2012 9109-9110 SYM denotes −
T2013 9110-9111 HYPH denotes /
T2014 9111-9112 SYM denotes −
T2015 9118-9119 -LRB- denotes (
T2016 9119-9125 NN denotes Figure
T2017 9126-9127 CD denotes 3
T2018 9127-9128 -RRB- denotes )
T2019 9128-9129 . denotes .
T2020 9129-9317 sentence denotes As a control analysis, anti–short-chain acyl-CoA dehydrogenase (SCAD) antisera revealed no differences in levels of expression of SCAD protein between MCAD+/+ and MCAD−/− mice (Figure 3).
T2021 9130-9132 IN denotes As
T2022 9209-9217 VBD denotes revealed
T2023 9133-9134 DT denotes a
T2024 9143-9151 NN denotes analysis
T2025 9135-9142 NN denotes control
T2026 9151-9153 , denotes ,
T2027 9153-9163 JJ denotes anti–short
T2028 9164-9169 NN denotes chain
T2029 9163-9164 HYPH denotes -
T2030 9179-9192 NN denotes dehydrogenase
T2031 9170-9174 NN denotes acyl
T2032 9175-9178 NN denotes CoA
T2033 9174-9175 HYPH denotes -
T2034 9194-9198 NN denotes SCAD
T2035 9193-9194 -LRB- denotes (
T2036 9200-9208 NNS denotes antisera
T2037 9198-9199 -RRB- denotes )
T2038 9218-9220 DT denotes no
T2039 9221-9232 NNS denotes differences
T2040 9233-9235 IN denotes in
T2041 9236-9242 NNS denotes levels
T2042 9243-9245 IN denotes of
T2043 9246-9256 NN denotes expression
T2044 9257-9259 IN denotes of
T2045 9260-9264 NN denotes SCAD
T2046 9265-9272 NN denotes protein
T2047 9273-9280 IN denotes between
T2048 9281-9285 NN denotes MCAD
T2049 9301-9305 NNS denotes mice
T2050 9285-9286 SYM denotes +
T2051 9286-9287 HYPH denotes /
T2052 9287-9288 SYM denotes +
T2053 9289-9292 CC denotes and
T2054 9293-9297 NN denotes MCAD
T2055 9297-9298 SYM denotes −
T2056 9298-9299 HYPH denotes /
T2057 9299-9300 SYM denotes −
T2058 9306-9307 -LRB- denotes (
T2059 9307-9313 NN denotes Figure
T2060 9314-9315 CD denotes 3
T2061 9315-9316 -RRB- denotes )
T2062 9316-9317 . denotes .
T2063 9317-10043 sentence denotes Figure 3 Immunoblots of Liver Homogenates from MCAD+/+ and MCAD−/− Mice These were probed with anti-MCAD antibody or anti-SCAD antibody. Homozygous MCAD−/− mice had no detectable MCAD protein. MCAD protein is only detectable under the MCAD protein–spiked (positive control) lane. As a control analysis, liver homogenates probed with anti-SCAD antibody revealed that SCAD protein was present in both MCAD+/+ and MCAD−/− mice. No MCAD positive-control protein is detected by anti-SCAD antibodies (MCAD lane). mw, molecular weight standards. Enzyme activity was analyzed in mouse liver homogenates using the electron transport flavoprotein (ETF) reduction assay with octanoyl-CoA (C8:0) and palmitoyl-CoA (C16:0) as substrates.
T2064 9858-9864 NN denotes Enzyme
T2065 9865-9873 NN denotes activity
T2066 9878-9886 VBN denotes analyzed
T2067 9874-9877 VBD denotes was
T2068 9887-9889 IN denotes in
T2069 9890-9895 NN denotes mouse
T2070 9902-9913 NNS denotes homogenates
T2071 9896-9901 NN denotes liver
T2072 9914-9919 VBG denotes using
T2073 9920-9923 DT denotes the
T2074 9972-9977 NN denotes assay
T2075 9924-9932 NN denotes electron
T2076 9943-9955 NN denotes flavoprotein
T2077 9933-9942 NN denotes transport
T2078 9956-9957 -LRB- denotes (
T2079 9957-9960 NN denotes ETF
T2080 9960-9961 -RRB- denotes )
T2081 9962-9971 NN denotes reduction
T2082 9978-9982 IN denotes with
T2083 9983-9991 NN denotes octanoyl
T2084 9992-9995 NN denotes CoA
T2085 9991-9992 HYPH denotes -
T2086 9996-9997 -LRB- denotes (
T2087 9997-9999 NN denotes C8
T2088 9999-10000 : denotes :
T2089 10000-10001 CD denotes 0
T2090 10001-10002 -RRB- denotes )
T2091 10003-10006 CC denotes and
T2092 10007-10016 NN denotes palmitoyl
T2093 10017-10020 NN denotes CoA
T2094 10016-10017 HYPH denotes -
T2095 10021-10022 -LRB- denotes (
T2096 10022-10025 NN denotes C16
T2097 10025-10026 : denotes :
T2098 10026-10027 CD denotes 0
T2099 10027-10028 -RRB- denotes )
T2100 10029-10031 IN denotes as
T2101 10032-10042 NNS denotes substrates
T2102 10042-10043 . denotes .
T2103 10043-10217 sentence denotes MCAD−/− mice had a significant reduction in ability to dehydrogenate octanoyl-CoA and a modest reduction in activity toward palmitoyl-CoA compared to MCAD+/+ mice (Table 1).
T2104 10044-10048 NN denotes MCAD
T2105 10052-10056 NNS denotes mice
T2106 10048-10049 SYM denotes −
T2107 10049-10050 HYPH denotes /
T2108 10050-10051 SYM denotes −
T2109 10057-10060 VBD denotes had
T2110 10061-10062 DT denotes a
T2111 10075-10084 NN denotes reduction
T2112 10063-10074 JJ denotes significant
T2113 10085-10087 IN denotes in
T2114 10088-10095 NN denotes ability
T2115 10096-10098 TO denotes to
T2116 10099-10112 VB denotes dehydrogenate
T2117 10113-10121 NN denotes octanoyl
T2118 10122-10125 NN denotes CoA
T2119 10121-10122 HYPH denotes -
T2120 10126-10129 CC denotes and
T2121 10130-10131 DT denotes a
T2122 10139-10148 NN denotes reduction
T2123 10132-10138 JJ denotes modest
T2124 10149-10151 IN denotes in
T2125 10152-10160 NN denotes activity
T2126 10161-10167 IN denotes toward
T2127 10168-10177 NN denotes palmitoyl
T2128 10178-10181 NN denotes CoA
T2129 10177-10178 HYPH denotes -
T2130 10182-10190 VBN denotes compared
T2131 10191-10193 IN denotes to
T2132 10194-10198 NN denotes MCAD
T2133 10202-10206 NNS denotes mice
T2134 10198-10199 SYM denotes +
T2135 10199-10200 HYPH denotes /
T2136 10200-10201 SYM denotes +
T2137 10207-10208 -LRB- denotes (
T2138 10208-10213 NN denotes Table
T2139 10214-10215 CD denotes 1
T2140 10215-10216 -RRB- denotes )
T2141 10216-10217 . denotes .
T2142 10217-10391 sentence denotes Specifically, the dehydrogenation of octanoyl-CoA and palmitoyl-CoA substrates were reduced by 75% and by 30%, respectively, in MCAD−/− mice as compared to MCAD+/+ controls.
T2143 10218-10230 RB denotes Specifically
T2144 10302-10309 VBN denotes reduced
T2145 10230-10232 , denotes ,
T2146 10232-10235 DT denotes the
T2147 10236-10251 NN denotes dehydrogenation
T2148 10252-10254 IN denotes of
T2149 10255-10263 NN denotes octanoyl
T2150 10264-10267 NN denotes CoA
T2151 10263-10264 HYPH denotes -
T2152 10286-10296 NNS denotes substrates
T2153 10268-10271 CC denotes and
T2155 10282-10285 NN denotes CoA
T2156 10281-10282 HYPH denotes -
T2157 10297-10301 VBD denotes were
T2158 10310-10312 IN denotes by
T2159 10313-10315 CD denotes 75
T2160 10315-10316 NN denotes %
T2161 10317-10320 CC denotes and
T2162 10321-10323 IN denotes by
T2163 10324-10326 CD denotes 30
T2164 10326-10327 NN denotes %
T2165 10327-10329 , denotes ,
T2437 11289-11290 SYM denotes +
T4108 17769-17770 SYM denotes −
T6638 26436-26446 RB denotes previously
T7247 5247-5255 NN denotes Strategy
T7248 5256-5259 IN denotes for
T7249 5260-5270 NN denotes Disruption
T7250 5271-5273 IN denotes of
T7251 5274-5277 DT denotes the
T7252 5290-5294 NN denotes Gene
T7253 5278-5283 NN denotes Mouse
T7254 5284-5289 NN denotes Acadm
T7255 5294-5412 sentence denotes (A) The MCAD IV2 insertion targeting vector with a deleted 1.3-kb region encompassing exon 10 and flanking sequences.
T7256 5295-5296 -LRB- denotes (
T7257 5296-5297 LS denotes A
T7258 5332-5338 NN denotes vector
T7259 5297-5298 -RRB- denotes )
T7260 5299-5302 DT denotes The
T7261 5303-5307 NN denotes MCAD
T7262 5312-5321 NN denotes insertion
T7263 5308-5311 NN denotes IV2
T7264 5322-5331 NN denotes targeting
T7265 5339-5343 IN denotes with
T7266 5344-5345 DT denotes a
T7267 5361-5367 NN denotes region
T7268 5346-5353 VBN denotes deleted
T7269 5354-5357 CD denotes 1.3
T7270 5358-5360 NN denotes kb
T7271 5357-5358 HYPH denotes -
T7272 5368-5380 VBG denotes encompassing
T7273 5381-5385 NN denotes exon
T7274 5386-5388 CD denotes 10
T7275 5389-5392 CC denotes and
T7276 5393-5401 VBG denotes flanking
T7277 5402-5411 NNS denotes sequences
T7278 5411-5412 . denotes .
T7279 5412-5576 sentence denotes MCAD IV2 undergoes gap repair upon homologous recombination at the endogenous Acadm locus resulting in a duplication of exons 8, 9, and 10 at the disrupted allele.
T7280 5413-5417 NN denotes MCAD
T7281 5418-5421 NN denotes IV2
T7282 5422-5431 VBZ denotes undergoes
T7283 5432-5435 NN denotes gap
T7284 5436-5442 NN denotes repair
T7285 5443-5447 IN denotes upon
T7286 5448-5458 JJ denotes homologous
T7287 5459-5472 NN denotes recombination
T7288 5473-5475 IN denotes at
T7289 5476-5479 DT denotes the
T7290 5497-5502 NN denotes locus
T7291 5480-5490 JJ denotes endogenous
T7292 5491-5496 NN denotes Acadm
T7293 5503-5512 VBG denotes resulting
T7294 5513-5515 IN denotes in
T7295 5516-5517 DT denotes a
T7296 5518-5529 NN denotes duplication
T7297 5530-5532 IN denotes of
T7298 5533-5538 NNS denotes exons
T7299 5539-5540 CD denotes 8
T7300 5540-5542 , denotes ,
T7301 5542-5543 CD denotes 9
T7302 5543-5545 , denotes ,
T7303 5545-5548 CC denotes and
T7304 5549-5551 CD denotes 10
T7305 5552-5554 IN denotes at
T7306 5555-5558 DT denotes the
T7307 5569-5575 NN denotes allele
T7308 5559-5568 VBN denotes disrupted
T7309 5575-5576 . denotes .
T7310 5576-5664 sentence denotes (B) Southern blot analysis of EcoRI-digested genomic DNA from ES cells screened by PCR.
T7311 5577-5578 -LRB- denotes (
T7312 5578-5579 LS denotes B
T7313 5595-5603 NN denotes analysis
T7314 5579-5580 -RRB- denotes )
T7315 5581-5589 NNP denotes Southern
T7316 5590-5594 NN denotes blot
T7317 5604-5606 IN denotes of
T7318 5607-5612 NN denotes EcoRI
T7319 5613-5621 VBN denotes digested
T7320 5612-5613 HYPH denotes -
T7321 5630-5633 NN denotes DNA
T7322 5622-5629 JJ denotes genomic
T7323 5634-5638 IN denotes from
T7324 5639-5641 NN denotes ES
T7325 5642-5647 NNS denotes cells
T7326 5648-5656 VBN denotes screened
T7327 5657-5659 IN denotes by
T7328 5660-5663 NN denotes PCR
T7329 5663-5664 . denotes .
T7330 5664-5871 sentence denotes Probe A, a DNA fragment consisting of a portion of exon 10 that is not present in the targeting vector, hybridizes to an endogenous 3.1-kb fragment and, upon homologous recombination, to a 13.2-kb fragment.
T7331 5665-5670 NN denotes Probe
T7332 5671-5672 NN denotes A
T7333 5769-5779 VBZ denotes hybridizes
T7334 5672-5674 , denotes ,
T7335 5674-5675 DT denotes a
T7336 5680-5688 NN denotes fragment
T7337 5676-5679 NN denotes DNA
T7338 5689-5699 VBG denotes consisting
T7339 5700-5702 IN denotes of
T7340 5703-5704 DT denotes a
T7341 5705-5712 NN denotes portion
T7342 5713-5715 IN denotes of
T7343 5716-5720 NN denotes exon
T7344 5721-5723 CD denotes 10
T7345 5724-5728 WDT denotes that
T7346 5729-5731 VBZ denotes is
T7347 5732-5735 RB denotes not
T7348 5736-5743 JJ denotes present
T7349 5744-5746 IN denotes in
T7350 5747-5750 DT denotes the
T7351 5761-5767 NN denotes vector
T7352 5751-5760 NN denotes targeting
T7353 5767-5769 , denotes ,
T7354 5780-5782 IN denotes to
T7355 5783-5785 DT denotes an
T7356 5804-5812 NN denotes fragment
T7357 5786-5796 JJ denotes endogenous
T7358 5797-5800 CD denotes 3.1
T7359 5801-5803 NN denotes kb
T7360 5800-5801 HYPH denotes -
T7361 5813-5816 CC denotes and
T7362 5816-5818 , denotes ,
T7363 5818-5822 IN denotes upon
T7364 5849-5851 IN denotes to
T7365 5823-5833 JJ denotes homologous
T7366 5834-5847 NN denotes recombination
T7367 5847-5849 , denotes ,
T7368 5852-5853 DT denotes a
T7369 5862-5870 NN denotes fragment
T7370 5854-5858 CD denotes 13.2
T7371 5859-5861 NN denotes kb
T7372 5858-5859 HYPH denotes -
T7373 5870-5871 . denotes .
T7374 5871-5966 sentence denotes Lane 1 represents a wild-type ES cell line, and Lane 2 and 3 represent targeted ES cell lines.
T7375 5872-5876 NN denotes Lane
T7376 5879-5889 VBZ denotes represents
T7377 5877-5878 CD denotes 1
T7378 5890-5891 DT denotes a
T7379 5910-5914 NN denotes line
T7380 5892-5896 JJ denotes wild
T7381 5897-5901 NN denotes type
T7382 5896-5897 HYPH denotes -
T7383 5902-5904 NN denotes ES
T7384 5905-5909 NN denotes cell
T7385 5914-5916 , denotes ,
T7386 5916-5919 CC denotes and
T7387 5920-5924 NN denotes Lane
T7388 5925-5926 CD denotes 2
T7389 5933-5942 VBP denotes represent
T7390 5927-5930 CC denotes and
T7391 5931-5932 CD denotes 3
T7392 5943-5951 VBN denotes targeted
T7393 5960-5965 NNS denotes lines
T7394 5952-5954 NN denotes ES
T7395 5955-5959 NN denotes cell
T7396 5965-5966 . denotes .
T7455 8568-8576 NNP denotes Northern
T7456 8577-8581 NN denotes Blot
T7457 8582-8590 NN denotes Analysis
T7458 8591-8595 IN denotes from
T7459 8596-8600 NN denotes MCAD
T7460 8632-8636 NNS denotes Mice
T7461 8600-8601 SYM denotes −
T7462 8601-8602 HYPH denotes /
T7463 8602-8603 SYM denotes −
T7464 8604-8605 -LRB- denotes (
T7465 8609-8610 CD denotes 2
T7466 8605-8606 NN denotes n
T7467 8607-8608 SYM denotes =
T7468 8610-8611 -RRB- denotes )
T7469 8612-8615 CC denotes and
T7470 8616-8620 NN denotes MCAD
T7471 8620-8621 SYM denotes +
T7472 8621-8622 HYPH denotes /
T7473 8622-8623 SYM denotes +
T7474 8624-8625 -LRB- denotes (
T7475 8629-8630 CD denotes 2
T7476 8625-8626 NN denotes n
T7477 8627-8628 SYM denotes =
T7478 8630-8631 -RRB- denotes )
T7479 8636-8788 sentence denotes Acadm message was detected from the heart, liver, brown fat, brain, kidney, and muscle (and white fat and testes, data not shown) of only MCAD+/+ mice.
T7480 8637-8642 NN denotes Acadm
T7481 8643-8650 NN denotes message
T7482 8655-8663 VBN denotes detected
T7483 8651-8654 VBD denotes was
T7484 8664-8668 IN denotes from
T7485 8669-8672 DT denotes the
T7486 8673-8678 NN denotes heart
T7487 8678-8680 , denotes ,
T7488 8680-8685 NN denotes liver
T7489 8685-8687 , denotes ,
T7490 8687-8692 JJ denotes brown
T7491 8693-8696 NN denotes fat
T7492 8696-8698 , denotes ,
T7493 8698-8703 NN denotes brain
T7494 8703-8705 , denotes ,
T7495 8705-8711 NN denotes kidney
T7496 8711-8713 , denotes ,
T7497 8713-8716 CC denotes and
T7498 8717-8723 NN denotes muscle
T7499 8724-8725 -LRB- denotes (
T7500 8725-8728 CC denotes and
T7501 8729-8734 JJ denotes white
T7502 8735-8738 NN denotes fat
T7503 8739-8742 CC denotes and
T7504 8743-8749 NNS denotes testes
T7505 8749-8751 , denotes ,
T7506 8760-8765 VBN denotes shown
T7507 8751-8755 NNS denotes data
T7508 8756-8759 RB denotes not
T7509 8765-8766 -RRB- denotes )
T7510 8767-8769 IN denotes of
T7511 8770-8774 JJ denotes only
T7512 8783-8787 NNS denotes mice
T7513 8775-8779 NN denotes MCAD
T7514 8779-8780 SYM denotes +
T7515 8780-8781 HYPH denotes /
T7516 8781-8782 SYM denotes +
T7517 8787-8788 . denotes .
T7518 8788-8870 sentence denotes Most robust expression occurred in brown fat, kidney, heart, and skeletal muscle.
T7519 8789-8793 RBS denotes Most
T7520 8801-8811 NN denotes expression
T7521 8794-8800 JJ denotes robust
T7522 8812-8820 VBD denotes occurred
T7523 8821-8823 IN denotes in
T7524 8824-8829 JJ denotes brown
T7525 8830-8833 NN denotes fat
T7526 8833-8835 , denotes ,
T7527 8835-8841 NN denotes kidney
T7528 8841-8843 , denotes ,
T7529 8843-8848 NN denotes heart
T7530 8848-8850 , denotes ,
T7531 8850-8853 CC denotes and
T7532 8854-8862 JJ denotes skeletal
T7533 8863-8869 NN denotes muscle
T7534 8869-8870 . denotes .
T7535 8870-8934 sentence denotes MCAD−/− mice had no detectable message in all tissues examined.
T7536 8871-8875 NN denotes MCAD
T7537 8879-8883 NNS denotes mice
T7538 8875-8876 SYM denotes −
T7539 8876-8877 HYPH denotes /
T7540 8877-8878 SYM denotes −
T7541 8884-8887 VBD denotes had
T7542 8888-8890 DT denotes no
T7543 8902-8909 NN denotes message
T7544 8891-8901 JJ denotes detectable
T7545 8910-8912 IN denotes in
T7546 8913-8916 DT denotes all
T7547 8917-8924 NNS denotes tissues
T7548 8925-8933 VBN denotes examined
T7549 8933-8934 . denotes .
T7638 9328-9339 NNS denotes Immunoblots
T7639 9340-9342 IN denotes of
T7640 9343-9348 NN denotes Liver
T7641 9349-9360 NNS denotes Homogenates
T7642 9361-9365 IN denotes from
T7643 9366-9370 NN denotes MCAD
T7644 9386-9390 NNS denotes Mice
T7645 9370-9371 SYM denotes +
T7646 9371-9372 HYPH denotes /
T7647 9372-9373 SYM denotes +
T7648 9374-9377 CC denotes and
T7649 9378-9382 NN denotes MCAD
T7650 9382-9383 SYM denotes −
T7651 9383-9384 HYPH denotes /
T7652 9384-9385 SYM denotes −
T7653 9390-9455 sentence denotes These were probed with anti-MCAD antibody or anti-SCAD antibody.
T7654 9391-9396 DT denotes These
T7655 9402-9408 VBN denotes probed
T7656 9397-9401 VBD denotes were
T7657 9409-9413 IN denotes with
T7658 9414-9423 JJ denotes anti-MCAD
T7659 9424-9432 NN denotes antibody
T7660 9433-9435 CC denotes or
T7661 9436-9445 JJ denotes anti-SCAD
T7662 9446-9454 NN denotes antibody
T7663 9454-9455 . denotes .
T7664 9455-9511 sentence denotes Homozygous MCAD−/− mice had no detectable MCAD protein.
T7665 9456-9466 JJ denotes Homozygous
T7666 9475-9479 NNS denotes mice
T7667 9467-9471 NN denotes MCAD
T7668 9471-9472 SYM denotes −
T7669 9472-9473 HYPH denotes /
T7670 9473-9474 SYM denotes −
T7671 9480-9483 VBD denotes had
T7672 9484-9486 DT denotes no
T7673 9503-9510 NN denotes protein
T7674 9487-9497 JJ denotes detectable
T7675 9498-9502 NN denotes MCAD
T7676 9510-9511 . denotes .
T7677 9511-9598 sentence denotes MCAD protein is only detectable under the MCAD protein–spiked (positive control) lane.
T7678 9512-9516 NN denotes MCAD
T7679 9517-9524 NN denotes protein
T7680 9525-9527 VBZ denotes is
T7681 9528-9532 RB denotes only
T7682 9533-9543 JJ denotes detectable
T7683 9544-9549 IN denotes under
T7684 9550-9553 DT denotes the
T7685 9593-9597 NN denotes lane
T7686 9554-9558 NN denotes MCAD
T7687 9559-9566 NN denotes protein
T7688 9567-9573 VBN denotes spiked
T7689 9566-9567 HYPH denotes –
T7690 9574-9575 -LRB- denotes (
T7691 9584-9591 NN denotes control
T7692 9575-9583 JJ denotes positive
T7693 9591-9592 -RRB- denotes )
T7694 9597-9598 . denotes .
T7695 9598-9743 sentence denotes As a control analysis, liver homogenates probed with anti-SCAD antibody revealed that SCAD protein was present in both MCAD+/+ and MCAD−/− mice.
T7696 9599-9601 IN denotes As
T7697 9671-9679 VBD denotes revealed
T7698 9602-9603 DT denotes a
T7699 9612-9620 NN denotes analysis
T7700 9604-9611 NN denotes control
T7701 9620-9622 , denotes ,
T7702 9622-9627 NN denotes liver
T7703 9628-9639 NNS denotes homogenates
T7704 9640-9646 VBN denotes probed
T7705 9647-9651 IN denotes with
T7706 9652-9661 JJ denotes anti-SCAD
T7707 9662-9670 NN denotes antibody
T7708 9680-9684 IN denotes that
T7709 9698-9701 VBD denotes was
T7710 9685-9689 NN denotes SCAD
T7711 9690-9697 NN denotes protein
T7712 9702-9709 JJ denotes present
T7713 9710-9712 IN denotes in
T7714 9713-9717 CC denotes both
T7715 9718-9722 NN denotes MCAD
T7716 9738-9742 NNS denotes mice
T7717 9722-9723 SYM denotes +
T7718 9723-9724 HYPH denotes /
T7719 9724-9725 SYM denotes +
T7720 9726-9729 CC denotes and
T7721 9730-9734 NN denotes MCAD
T7722 9734-9735 SYM denotes −
T7723 9735-9736 HYPH denotes /
T7724 9736-9737 SYM denotes −
T7725 9742-9743 . denotes .
T7726 9743-9825 sentence denotes No MCAD positive-control protein is detected by anti-SCAD antibodies (MCAD lane).
T7727 9744-9746 DT denotes No
T7728 9769-9776 NN denotes protein
T7729 9747-9751 NN denotes MCAD
T7730 9752-9760 JJ denotes positive
T7731 9761-9768 NN denotes control
T7732 9760-9761 HYPH denotes -
T7733 9780-9788 VBN denotes detected
T7734 9777-9779 VBZ denotes is
T7735 9789-9791 IN denotes by
T7736 9792-9801 JJ denotes anti-SCAD
T7737 9802-9812 NNS denotes antibodies
T7738 9813-9814 -LRB- denotes (
T7739 9819-9823 NN denotes lane
T7740 9814-9818 NN denotes MCAD
T7741 9823-9824 -RRB- denotes )
T7742 9824-9825 . denotes .
T7743 9825-9857 sentence denotes mw, molecular weight standards.
T7744 9826-9828 NN denotes mw
T7745 9828-9830 , denotes ,
T7746 9830-9839 JJ denotes molecular
T7747 9847-9856 NNS denotes standards
T7748 9840-9846 NN denotes weight
T7749 9856-9857 . denotes .
T8117 15442-15443 . denotes .
T8161 10401-10416 NNS denotes Characteristics
T8162 10417-10419 IN denotes of
T8163 10420-10424 NN denotes MCAD
T8164 10425-10434 JJ denotes Deficient
T8165 10424-10425 HYPH denotes -
T8166 10435-10439 NNS denotes Mice
T8116 15436-15437 SYM denotes −
T8115 15435-15436 HYPH denotes /
T8114 15434-15435 SYM denotes −
T8113 15438-15442 NNS denotes mice
T8112 15430-15434 NN denotes MCAD
T8111 15427-15429 IN denotes in
T8110 15418-15426 NN denotes necrosis
T8109 15414-15417 CC denotes and
T8108 15393-15400 NN denotes myocyte
T8107 15382-15392 JJ denotes multifocal
T8106 15375-15381 JJ denotes active
T8105 15401-15413 NN denotes degeneration
T8104 15367-15374 JJ denotes chronic
T8103 15362-15366 IN denotes with
T8102 15339-15346 JJ denotes Diffuse
T8101 15337-15338 -RRB- denotes )
T8100 15336-15337 LS denotes D
T8099 15332-15335 CC denotes and
T8098 15347-15361 NN denotes cardiomyopathy
T8097 15330-15331 LS denotes C
T8096 15329-15330 -LRB- denotes (
T8095 15328-15443 sentence denotes (C and D) Diffuse cardiomyopathy with chronic active multifocal myocyte degeneration and necrosis in MCAD−/− mice.
T8042 15101-15102 . denotes .
T8041 15094-15095 HYPH denotes -
T8040 15095-15096 NN denotes h
T8039 15092-15094 CD denotes 24
T8038 15097-15101 NN denotes fast
T8037 15090-15091 DT denotes a
T8036 15080-15089 VBG denotes following
T8035 15070-15079 NN denotes steatosis
T8034 15062-15069 JJ denotes hepatic
T8033 15059-15061 IN denotes of
T8032 15050-15058 NN denotes evidence
T8031 15047-15049 DT denotes no
T8030 15036-15037 SYM denotes +
T8029 15035-15036 HYPH denotes /
T8028 15034-15035 SYM denotes +
T8027 15038-15042 NNS denotes mice
T8026 15030-15034 NN denotes MCAD
T8025 15028-15029 -RRB- denotes )
T8024 15043-15046 VBD denotes had
T8023 15027-15028 LS denotes A
T8022 15026-15027 -LRB- denotes (
T8021 15025-15102 sentence denotes (A) MCAD+/+ mice had no evidence of hepatic steatosis following a 24-h fast.
T8020 15019-15020 SYM denotes −
T8019 15018-15019 HYPH denotes /
T8018 15017-15018 SYM denotes −
T8017 15013-15017 NN denotes MCAD
T8016 15009-15012 CC denotes and
T8015 15007-15008 SYM denotes +
T8014 15006-15007 HYPH denotes /
T8013 15005-15006 SYM denotes +
T8012 15021-15025 NNS denotes Mice
T8011 15001-15005 NN denotes MCAD
T8010 14998-15000 IN denotes of
T8009 14983-14997 NN denotes Histopathology
T7957 14405-14406 . denotes .
T7956 14397-14405 NN denotes asterisk
T7955 14394-14396 DT denotes an
T7954 14391-14393 IN denotes by
T7953 14377-14380 VBP denotes are
T7952 14381-14390 VBN denotes indicated
T7951 14367-14376 NNS denotes standards
T7950 14358-14366 JJ denotes Internal
T7949 14357-14406 sentence denotes Internal standards are indicated by an asterisk.
T7948 14356-14357 . denotes .
T7947 14346-14356 NN denotes deficiency
T7946 14341-14345 NN denotes MCAD
T7945 14336-14340 IN denotes with
T7944 14322-14327 JJ denotes human
T7943 14328-14335 NN denotes patient
T7942 14320-14321 DT denotes a
T7941 14317-14319 IN denotes to
T7940 14308-14316 VBN denotes compared
T7939 14300-14301 SYM denotes −
T7938 14299-14300 HYPH denotes /
T7937 14298-14299 SYM denotes −
T7936 14294-14298 NN denotes MCAD
T7935 14302-14307 NN denotes mouse
T7934 14291-14293 DT denotes an
T7933 14288-14290 IN denotes of
T7932 14266-14279 NN denotes acylcarnitine
T7931 14261-14265 NN denotes Bile
T7930 14259-14260 -RRB- denotes )
T7929 14280-14287 NN denotes profile
T7928 14258-14259 LS denotes C
T7927 14257-14258 -LRB- denotes (
T7926 14256-14357 sentence denotes (C) Bile acylcarnitine profile of an MCAD−/− mouse compared to a human patient with MCAD deficiency.
T7925 14255-14256 . denotes .
T7924 14250-14255 CD denotes 0.001
T7923 14248-14249 SYM denotes <
T7922 14246-14247 NN denotes p
T7921 14236-14245 VBZ denotes indicates
T7920 14227-14235 NN denotes Asterisk
T7919 14226-14256 sentence denotes Asterisk indicates p < 0.001.
T7918 14225-14226 . denotes .
T7917 14223-14225 NN denotes SD
T7916 14221-14222 SYM denotes ±
T7915 14214-14220 NNS denotes values
T7914 14209-14213 NN denotes mean
T7913 14199-14204 VBN denotes shown
T7912 14205-14208 VBP denotes are
T7911 14192-14198 NNS denotes Values
T7910 14191-14226 sentence denotes Values shown are mean values ± SD.
T7909 14190-14191 . denotes .
T7908 14181-14190 VBN denotes indicated
T7907 14178-14180 IN denotes as
T7906 14176-14177 -RRB- denotes )
T7905 14175-14176 NN denotes A
T7904 14174-14175 -LRB- denotes (
T7903 14171-14173 IN denotes in
T7902 14165-14170 VBN denotes shown
T7901 14155-14159 JJ denotes same
T7900 14160-14164 NNS denotes mice
T7899 14151-14154 DT denotes the
T7898 14148-14150 IN denotes of
T7897 14133-14147 NNS denotes acylcarnitines
T7896 14128-14132 NN denotes bile
T7895 14125-14127 IN denotes in
T7894 14114-14124 NNS denotes elevations
T7893 14102-14113 JJ denotes significant
T7892 14092-14097 EX denotes There
T7891 14090-14091 -RRB- denotes )
T7890 14098-14101 VBP denotes are
T7889 14089-14090 LS denotes B
T7888 14088-14089 -LRB- denotes (
T7887 14087-14191 sentence denotes (B) There are significant elevations in bile acylcarnitines of the same mice shown in (A) as indicated.
T7886 14086-14087 . denotes .
T7885 14082-14086 CD denotes 0.01
T7884 14080-14081 SYM denotes <
T7883 14078-14079 NN denotes p
T7882 14068-14077 VBZ denotes indicates
T7881 14066-14067 SYM denotes ‡
T7880 14062-14065 CC denotes and
T7879 14056-14061 CD denotes 0.002
T7878 14054-14055 SYM denotes <
T7877 14052-14053 NN denotes p
T7876 14042-14051 VBZ denotes indicates
T7875 14033-14041 NN denotes Asterisk
T7874 14032-14087 sentence denotes Asterisk indicates p < 0.002 and ‡ indicates p < 0.01.
T7873 14031-14032 . denotes .
T7872 14030-14031 -RRB- denotes )
T7871 14028-14030 NN denotes SD
T7870 14027-14028 -LRB- denotes (
T7869 14017-14026 NN denotes deviation
T7868 14008-14016 JJ denotes standard
T7867 14006-14007 SYM denotes ±
T7866 13999-14005 NNS denotes values
T7865 13994-13998 NN denotes mean
T7864 13984-13989 VBN denotes shown
T7863 13990-13993 VBP denotes are
T7862 13977-13983 NNS denotes Values
T7861 13976-14032 sentence denotes Values shown are mean values ± standard deviation (SD).
T7860 13975-13976 . denotes .
T7859 13969-13970 SYM denotes −
T7858 13968-13969 HYPH denotes /
T7857 13967-13968 SYM denotes −
T7856 13971-13975 NNS denotes mice
T7855 13963-13967 NN denotes MCAD
T7854 13960-13962 IN denotes in
T7853 13950-13959 VBN denotes indicated
T7852 13947-13949 IN denotes as
T7851 13939-13946 NNS denotes species
T7850 13925-13938 NN denotes acylcarnitine
T7849 13922-13924 IN denotes in
T7848 13911-13921 NNS denotes elevations
T7847 13899-13910 JJ denotes significant
T7846 13895-13898 VBP denotes are
T7845 13889-13894 EX denotes There
T7844 13888-13976 sentence denotes There are significant elevations in acylcarnitine species as indicated in MCAD−/− mice.
T7843 13887-13888 -RRB- denotes )
T7842 13884-13885 SYM denotes =
T7841 13882-13883 NN denotes n
T7840 13886-13887 CD denotes 4
T7839 13881-13882 -LRB- denotes (
T7838 13874-13875 SYM denotes −
T7837 13873-13874 HYPH denotes /
T7836 13872-13873 SYM denotes −
T7835 13868-13872 NN denotes MCAD
T7834 13864-13867 CC denotes and
T7833 13862-13863 -RRB- denotes )
T7832 13859-13860 SYM denotes =
T7831 13857-13858 NN denotes n
T7830 13861-13862 CD denotes 4
T7829 13856-13857 -LRB- denotes (
T7828 13854-13855 SYM denotes +
T7827 13853-13854 HYPH denotes /
T8094 15327-15328 . denotes .
T8093 15321-15322 SYM denotes −
T8092 15320-15321 HYPH denotes /
T8091 15319-15320 SYM denotes −
T8090 15323-15327 NNS denotes mice
T8089 15315-15319 NN denotes MCAD
T8088 15312-15314 IN denotes in
T8087 15294-15301 JJ denotes hepatic
T8086 15279-15293 JJ denotes macrovesicular
T8085 15275-15278 CC denotes and
T8084 15260-15274 JJ denotes microvesicular
T8083 15252-15259 JJ denotes diffuse
T8082 15248-15251 CC denotes and
T8081 15302-15311 NN denotes steatosis
T8080 15241-15247 JJ denotes severe
T8079 15232-15240 VBD denotes revealed
T8078 15217-15222 NN denotes liver
T8077 15223-15231 NNS denotes sections
T8076 15209-15216 VBN denotes stained
T8075 15203-15206 JJ denotes Red
T8074 15202-15203 HYPH denotes -
T8073 15207-15208 NN denotes O
T8072 15199-15202 NN denotes Oil
T8071 15198-15328 sentence denotes Oil-Red O stained liver sections revealed severe and diffuse microvesicular and macrovesicular hepatic steatosis in MCAD−/− mice.
T8070 15197-15198 . denotes .
T8069 15190-15191 HYPH denotes -
T8068 15191-15192 NN denotes h
T8067 15188-15190 CD denotes 24
T8066 15193-15197 NN denotes fast
T8065 15186-15187 DT denotes a
T8064 15176-15185 VBG denotes following
T8063 15170-15175 NN denotes mouse
T8062 15167-15168 HYPH denotes /
T8061 15166-15167 NN denotes −
T8060 15168-15169 NN denotes −
T8059 15162-15166 NN denotes MCAD
T8058 15159-15161 IN denotes in
T8057 15141-15142 -RRB- denotes )
T8056 15143-15158 NN denotes Hepatosteatosis
T8055 15140-15141 LS denotes B
T8054 15139-15140 -LRB- denotes (
T8053 15138-15198 sentence denotes (B) Hepatosteatosis in MCAD−/− mouse following a 24-h fast.
T8052 15137-15138 . denotes .
T8051 15132-15137 NN denotes stain
T8050 15126-15129 JJ denotes Red
T8049 15125-15126 HYPH denotes -
T8048 15130-15131 NN denotes O
T8047 15122-15125 NN denotes Oil
T8046 15117-15121 IN denotes with
T8045 15109-15116 NN denotes section
T8044 15103-15108 NN denotes Liver
T8043 15102-15138 sentence denotes Liver section with Oil-Red O stain.
T7229 28553-28554 . denotes .
T7228 28542-28553 JJ denotes significant
T7227 28539-28541 IN denotes as
T7226 28531-28534 VBD denotes was
T7225 28526-28530 CD denotes 0.05
T7224 28524-28525 SYM denotes <
T7223 28535-28538 VBN denotes set
T7222 28522-28523 NN denotes p
T7221 28520-28521 DT denotes A
T7220 28519-28554 sentence denotes A p < 0.05 was set as significant.
T7219 28518-28519 . denotes .
T7218 28513-28514 HYPH denotes -
T7217 28512-28513 NN denotes t
T7216 28509-28511 POS denotes 's
T7215 28514-28518 NN denotes test
T7214 28502-28509 NNP denotes Student
T7213 28496-28501 VBG denotes using
T7212 28483-28495 NN denotes significance
T7211 28471-28482 JJ denotes statistical
T7210 28467-28470 IN denotes for
T7209 28455-28459 VBD denotes were
T7208 28448-28454 NNS denotes groups
T7207 28440-28447 IN denotes between
T7206 28460-28466 VBN denotes tested
T7205 28432-28439 NNS denotes Results
T7204 28431-28519 sentence denotes Results between groups were tested for statistical significance using Student's t-test.
T7203 28430-28431 . denotes .
T7202 28422-28430 NNS denotes analyses
T7201 28410-28421 JJ denotes Statistical
T7137 28407-28408 . denotes .
T7136 28400-28407 NNS denotes lesions
T7135 28392-28399 JJ denotes cardiac
T7134 28388-28391 IN denotes for
T7133 28373-28377 VBD denotes were
T7132 28353-28360 JJ denotes cardiac
T7131 28361-28372 NN denotes enlargement
T7130 28345-28352 JJ denotes visible
T7129 28340-28344 IN denotes with
T7128 28378-28387 VBN denotes evaluated
T7127 28334-28339 DT denotes Those
T7126 28333-28408 sentence denotes Those with visible cardiac enlargement were evaluated for cardiac lesions.
T7125 28332-28333 . denotes .
T7124 28316-28324 JJ denotes clinical
T7123 28325-28332 NN denotes disease
T7122 28307-28315 JJ denotes sporadic
T7121 28304-28306 IN denotes of
T7120 28296-28303 IN denotes because
T7119 28282-28286 VBD denotes were
T7118 28287-28295 VBN denotes examined
T7117 28277-28281 NNS denotes mice
T7116 28271-28276 JJ denotes Other
T7115 28270-28333 sentence denotes Other mice were examined because of sporadic clinical disease.
T7114 28269-28270 . denotes .
T7113 28266-28269 NN denotes age
T7112 28263-28265 CC denotes or
T7111 28254-28262 NN denotes genotype
T7110 28251-28253 IN denotes of
T7109 28241-28250 NN denotes knowledge
T7108 28233-28240 IN denotes without
T7107 28219-28223 VBD denotes were
T7106 28224-28232 VBN denotes examined
T7105 28212-28218 NNS denotes Slides
T7104 28211-28270 sentence denotes Slides were examined without knowledge of genotype or age.
T7103 28210-28211 . denotes .
T7102 28208-28209 HYPH denotes -
T7101 28205-28208 JJ denotes red
T7100 28204-28205 HYPH denotes -
T7099 28209-28210 NN denotes O
T7098 28201-28204 NN denotes Oil
T7097 28196-28200 IN denotes with
T7096 28183-28187 VBD denotes were
T7095 28188-28195 VBN denotes stained
T7094 28174-28182 NNS denotes sections
T7093 28170-28173 CC denotes and
T7092 28162-28169 NNS denotes methods
T7091 28153-28161 JJ denotes standard
T7090 28147-28152 VBG denotes using
T7089 28133-28137 VBD denotes were
T7088 28138-28146 VBN denotes prepared
T7087 28118-28123 NN denotes liver
T7086 28124-28132 NNS denotes sections
T7085 28111-28117 VBN denotes Frozen
T7084 28110-28211 sentence denotes Frozen liver sections were prepared using standard methods and sections were stained with Oil-red-O.
T7826 13852-13853 SYM denotes +
T7825 13876-13880 NNS denotes mice
T7824 13848-13852 NN denotes MCAD
T7823 13845-13847 IN denotes of
T7822 13822-13835 NN denotes acylcarnitine
T7821 13816-13821 NN denotes Serum
T7820 13814-13815 -RRB- denotes )
T7819 13836-13844 NN denotes analysis
T7818 13813-13814 LS denotes A
T7817 13812-13813 -LRB- denotes (
T7816 13811-13888 sentence denotes (A) Serum acylcarnitine analysis of MCAD+/+ (n = 4) and MCAD−/− mice (n = 4)
T7815 13803-13811 NNS denotes Analyses
T7814 13789-13802 NN denotes Acylcarnitine
T7083 28109-28110 . denotes .
T7082 28104-28109 NN denotes eosin
T7081 28100-28103 CC denotes and
T7080 28088-28099 NN denotes hematoxylin
T7079 28083-28087 IN denotes with
T7078 28075-28082 VBN denotes stained
T7077 28071-28074 CC denotes and
T7076 28069-28071 , denotes ,
T7075 28067-28069 NN denotes μm
T7074 28065-28066 CD denotes 5
T7073 28062-28064 IN denotes at
T7072 28052-28061 VBN denotes sectioned
T7071 28050-28052 , denotes ,
T7070 28040-28050 NN denotes sectioning
T7069 28031-28039 NN denotes paraffin
T7068 28027-28030 IN denotes for
T7067 28017-28026 RB denotes routinely
T7066 28007-28016 VBN denotes processed
T7065 28005-28007 , denotes ,
T7064 27995-27996 NN denotes %
T7063 27993-27995 CD denotes 10
T7062 27997-28005 NN denotes formalin
T7061 27984-27992 VBN denotes buffered
T7060 27981-27983 IN denotes in
T7059 27971-27980 NN denotes immersion
T7058 27968-27970 IN denotes by
T6907 27580-27581 . denotes .
T6906 27579-27580 -RRB- denotes ]
T6905 27576-27577 , denotes ,
T6904 27574-27576 CD denotes 31
T6903 27577-27579 CD denotes 32
T6902 27573-27574 -LRB- denotes [
T6901 27555-27559 NN denotes mass
T6900 27548-27554 JJ denotes tandem
T6899 27560-27572 NN denotes spectrometry
T6898 27535-27547 NN denotes electrospray
T6897 27529-27534 VBG denotes using
T6896 27514-27518 VBD denotes were
T6895 27509-27513 NN denotes bile
T6894 27505-27508 CC denotes and
T6893 27499-27504 NN denotes serum
T6892 27496-27498 IN denotes in
T6891 27519-27528 VBN denotes conducted
T6890 27487-27495 NNS denotes analyses
T6889 27473-27486 NN denotes Acylcarnitine
T6888 27472-27581 sentence denotes Acylcarnitine analyses in serum and bile were conducted using electrospray tandem mass spectrometry [31,32].
T6887 27471-27472 . denotes .
T6886 27450-27461 NN denotes calibration
T6885 27462-27471 NNS denotes standards
T6884 27440-27449 JJ denotes synthetic
T6883 27435-27439 IN denotes with
T6882 27424-27434 NN denotes comparison
T6881 27421-27423 IN denotes on
T6880 27415-27420 VBN denotes based
T6879 27405-27409 VBD denotes were
T6878 27410-27414 VBN denotes made
T6877 27390-27404 NNS denotes determinations
T6876 27377-27389 JJ denotes quantitative
T6875 27373-27376 CC denotes and
T6874 27371-27373 , denotes ,
T6873 27354-27362 JJ denotes internal
T6872 27363-27371 NN denotes standard
T6871 27350-27353 DT denotes the
T6870 27347-27349 IN denotes as
T6869 27338-27341 VBD denotes was
T6868 27336-27337 -RRB- denotes )
T6867 27330-27336 NNP denotes States
T6866 27323-27329 NNP denotes United
T6865 27321-27323 , denotes ,
T6864 27313-27321 NNP denotes Missouri
T6863 27311-27313 , denotes ,
T6862 27306-27311 NNP denotes Louis
T6861 27302-27305 NNP denotes St.
T6860 27300-27302 , denotes ,
T6859 27293-27294 : denotes ;
T6858 27290-27293 NN denotes C24
T6857 27295-27300 NNP denotes Sigma
T6856 27289-27290 -LRB- denotes (
T6855 27342-27346 VBN denotes used
T6854 27277-27288 NN denotes tetracosane
T6853 27270-27276 IN denotes except
T6852 27268-27270 , denotes ,
T6851 27267-27268 -RRB- denotes ]
T6850 27264-27265 , denotes ,
T6849 27262-27264 CD denotes 22
T6848 27265-27267 CD denotes 30
T6847 27261-27262 -LRB- denotes [
T6846 27240-27250 RB denotes previously
T6845 27251-27260 VBN denotes described
T6844 27237-27239 IN denotes as
T6843 27224-27236 NN denotes spectroscopy
T6842 27219-27223 NN denotes mass
T6841 27218-27219 HYPH denotes -
T6840 27204-27218 NN denotes chromatography
T6813 27070-27072 NN denotes μl
T6812 27066-27067 -LRB- denotes (
T6811 27054-27060 NN denotes sample
T6810 27046-27053 VBN denotes reduced
T6809 27061-27065 NN denotes size
T6808 27044-27045 DT denotes a
T6807 27032-27043 VB denotes accommodate
T6806 27029-27031 TO denotes to
T6805 27016-27019 VBD denotes was
T6804 27020-27028 VBN denotes modified
T6803 27010-27015 NN denotes assay
T6802 27006-27009 DT denotes The
T6801 27005-27150 sentence denotes The assay was modified to accommodate a reduced sample size (10 μl) and use of a microplate reader for measurement of optical density at 550 nm.
T6800 27004-27005 . denotes .
T6799 27003-27004 -RRB- denotes )
T6798 26997-27003 NNP denotes States
T6797 26990-26996 NNP denotes United
T6796 26988-26990 , denotes ,
T6795 26980-26988 NNP denotes Virginia
T6794 26978-26980 , denotes ,
T6793 26970-26978 NNP denotes Richmond
T6792 26968-26970 , denotes ,
T6791 26952-26956 NNP denotes Wako
T6790 26950-26952 , denotes ,
T6789 26940-26941 '' denotes ”
T6788 26942-26950 NNS denotes reagents
T6787 26938-26939 HYPH denotes -
T6786 26939-26940 NN denotes C
T6785 26934-26938 NN denotes NEFA
T6784 26933-26934 `` denotes “
T6783 26957-26968 NNP denotes Diagnostics
T6782 26932-26933 -LRB- denotes (
T6781 26912-26924 JJ denotes colorimetric
T6780 26910-26912 , denotes ,
T6779 26901-26910 JJ denotes enzymatic
T6778 26925-26931 NN denotes method
T6777 26898-26900 DT denotes an
T6776 26895-26897 IN denotes by
T6775 26881-26885 VBD denotes were
T6774 26879-26880 -RRB- denotes )
T6773 26875-26879 NN denotes NEFA
T6772 26874-26875 -LRB- denotes (
T6771 26886-26894 VBN denotes measured
T6770 26862-26867 JJ denotes fatty
T6769 26847-26861 JJ denotes non-esterified
T6768 26868-26873 NNS denotes acids
T6767 26841-26846 JJ denotes Total
T6766 26840-27005 sentence denotes Total non-esterified fatty acids (NEFA) were measured by an enzymatic, colorimetric method (“NEFA-C” reagents, Wako Diagnostics, Richmond, Virginia, United States).
T6765 26839-26840 . denotes .
T6764 26838-26839 -RRB- denotes )
T6763 26832-26838 NNP denotes States
T6762 26825-26831 NNP denotes United
T6761 26823-26825 , denotes ,
T6760 26819-26823 NNP denotes York
T6759 26815-26818 NNP denotes New
T6758 26813-26815 , denotes ,
T6757 26804-26813 NNP denotes Rochester
T6756 26802-26804 , denotes ,
T6755 26782-26790 NNP denotes Clinical
T6754 26774-26781 NNP denotes Johnson
T6753 26770-26773 CC denotes and
T6752 26762-26769 NNP denotes Johnson
T6751 26791-26802 NNP denotes Diagnostics
T6750 26761-26762 -LRB- denotes (
T6749 26751-26753 CD denotes II
T6748 26748-26750 NN denotes DT
T6747 26739-26747 NN denotes Ektachem
T6746 26754-26760 NN denotes system
T6745 26736-26738 DT denotes an
T6744 26730-26735 VBG denotes using
T6743 26725-26729 NNS denotes sera
T6742 26722-26724 NN denotes μl
T6741 26719-26721 CD denotes 10
T6740 26716-26718 IN denotes in
T6739 26703-26706 VBD denotes was
T6738 26707-26715 VBN denotes measured
T6737 26689-26702 NN denotes concentration
T6736 26681-26688 NN denotes Glucose
T6637 26447-26456 VBN denotes described
T6636 26433-26435 IN denotes as
T6635 26423-26432 NN denotes substrate
T6634 26420-26422 IN denotes as
T6633 26418-26419 -RRB- denotes )
T6632 26415-26418 NN denotes C16
T6631 26414-26415 -LRB- denotes (
T6630 26409-26410 HYPH denotes -
T6629 26410-26413 NN denotes CoA
T6628 26400-26409 NN denotes palmitoyl
T6627 26396-26399 CC denotes and
T6626 26394-26395 -RRB- denotes )
T6625 26392-26394 NN denotes C8
T6624 26391-26392 -LRB- denotes (
T6623 26386-26387 HYPH denotes -
T6622 26387-26390 NN denotes CoA
T6621 26378-26386 NN denotes octanoyl
T6620 26373-26377 IN denotes with
T6619 26364-26372 NNS denotes extracts
T6618 26357-26363 NN denotes tissue
T6617 26354-26356 IN denotes on
T6616 26345-26348 VBD denotes was
T6615 26349-26353 VBN denotes used
T6614 26329-26338 NN denotes reduction
T6613 26325-26328 NN denotes ETF
T6612 26315-26324 JJ denotes anaerobic
T6611 26306-26314 JJ denotes specific
T6610 26299-26305 RB denotes highly
T6609 26295-26298 CC denotes and
T6608 26285-26294 JJ denotes sensitive
T6607 26339-26344 NN denotes assay
T6606 26281-26284 DT denotes The
T6605 26280-26462 sentence denotes The sensitive and highly specific anaerobic ETF reduction assay was used on tissue extracts with octanoyl-CoA (C8) and palmitoyl-CoA (C16) as substrate as previously described [29].
T6604 26279-26280 . denotes .
T6603 26278-26279 -RRB- denotes )
T6602 26275-26276 SYM denotes =
T6601 26273-26274 NN denotes n
T6600 26277-26278 CD denotes 5
T6599 26272-26273 -LRB- denotes (
T6598 26265-26266 SYM denotes −
T6597 26264-26265 HYPH denotes /
T6596 26263-26264 SYM denotes −
T6595 26259-26263 NN denotes MCAD
T6594 26255-26258 CC denotes and
T6593 26253-26254 -RRB- denotes )
T6592 26250-26251 SYM denotes =
T6591 26248-26249 NN denotes n
T6590 26252-26253 CD denotes 5
T6589 26247-26248 -LRB- denotes (
T6588 26245-26246 SYM denotes +
T6587 26244-26245 HYPH denotes /
T6586 26243-26244 SYM denotes +
T6585 26267-26271 NNS denotes mice
T6584 26239-26243 NN denotes MCAD
T6583 26234-26238 IN denotes from
T6582 26220-26224 VBD denotes were
T6581 26208-26219 NNS denotes homogenates
T6580 26202-26207 NN denotes liver
T6579 26200-26202 , denotes ,
T6578 26196-26200 NNS denotes mice
T6577 26193-26195 IN denotes in
T6576 26184-26192 NN denotes activity
T6575 26179-26183 NN denotes MCAD
T6574 26170-26178 VB denotes evaluate
T6573 26167-26169 TO denotes to
T6572 26161-26166 NN denotes order
T6571 26225-26233 VBN denotes prepared
T6570 26158-26160 IN denotes In
T6569 26157-26280 sentence denotes In order to evaluate MCAD activity in mice, liver homogenates were prepared from MCAD+/+ (n = 5) and MCAD−/− mice (n = 5).
T6568 26156-26157 . denotes .
T6567 26148-26156 NN denotes activity
T6566 26141-26147 NN denotes enzyme
T6565 26135-26140 NN denotes Liver
T6488 26132-26133 . denotes .
T6487 26131-26132 -RRB- denotes )
T6486 26125-26131 NNP denotes States
T6485 26118-26124 NNP denotes United
T6484 26116-26118 , denotes ,
T6483 26103-26116 NNP denotes Massachusetts
T6482 26101-26103 , denotes ,
T6481 26095-26101 NNP denotes Boston
T6480 26093-26095 , denotes ,
T6479 26072-26084 NNP denotes Lifesciences
T6478 26085-26093 NNP denotes Products
T6477 26068-26071 NNP denotes NEN
T6476 26066-26068 , denotes ,
T6475 26055-26066 NNP denotes Renaissance
T6474 26054-26055 -LRB- denotes (
T6473 26036-26053 NN denotes chemiluminiscence
T6472 26033-26035 IN denotes by
T6471 26023-26032 VBN denotes developed
T6470 26019-26022 CC denotes and
T6469 26008-26018 NNS denotes procedures
T6468 25999-26007 JJ denotes standard
T6467 25993-25998 VBG denotes using
T6466 25981-25992 NN denotes temperature
T6465 25976-25980 NN denotes room
T6464 25973-25975 IN denotes at
T6463 25971-25972 NN denotes h
T6462 25968-25969 SYM denotes –
T6461 25969-25970 CD denotes 4
T6460 25967-25968 CD denotes 2
T6459 25963-25966 IN denotes for
T6458 25944-25953 JJ denotes secondary
T6457 25932-25933 HYPH denotes -
T6456 25933-25943 VBN denotes conjugated
T6455 25929-25932 NN denotes HRP
T6454 25925-25928 NN denotes IgG
T6453 25954-25962 NN denotes antibody
T6452 25914-25924 JJ denotes anti-mouse
T6451 25911-25913 IN denotes in
T6450 25896-25900 VBD denotes were
T6449 25901-25910 VBN denotes incubated
T7057 27957-27961 VBD denotes were
T7056 27950-27956 NN denotes muscle
T7055 27941-27949 JJ denotes skeletal
T7054 27937-27940 CC denotes and
T7053 27935-27937 , denotes ,
T7052 27928-27935 NNS denotes ovaries
T7051 27926-27928 , denotes ,
T7050 27917-27926 NNS denotes testicles
T7049 27915-27917 , denotes ,
T7048 27910-27915 NN denotes heart
T7047 27908-27910 , denotes ,
T7046 27903-27908 NN denotes brain
T7045 27901-27903 , denotes ,
T7044 27896-27901 NN denotes liver
T7043 27894-27896 , denotes ,
T7042 27886-27894 NN denotes pancreas
T7041 27884-27886 , denotes ,
T7040 27878-27884 NN denotes spleen
T7039 27876-27878 , denotes ,
T7038 27962-27967 VBN denotes fixed
T7037 27870-27876 NN denotes Kidney
T7036 27869-28110 sentence denotes Kidney, spleen, pancreas, liver, brain, heart, testicles, ovaries, and skeletal muscle were fixed by immersion in buffered 10% formalin, processed routinely for paraffin sectioning, sectioned at 5 μm, and stained with hematoxylin and eosin.
T7035 27868-27869 . denotes .
T7034 27864-27865 HYPH denotes -
T7033 27865-27868 JJ denotes old
T7032 27861-27862 HYPH denotes -
T7031 27862-27864 NN denotes wk
T7030 27860-27861 CD denotes 4
T7029 27853-27854 SYM denotes +
T7028 27852-27853 HYPH denotes /
T7027 27851-27852 SYM denotes +
T7026 27847-27851 NN denotes MCAD
T7025 27840-27846 JJ denotes female
T7024 27855-27859 NNS denotes mice
T7023 27836-27839 CD denotes two
T7022 27832-27835 CC denotes and
T7021 27827-27831 JJ denotes male
T7020 27823-27826 CD denotes two
T7019 27819-27822 CC denotes and
T7018 27817-27819 , denotes ,
T7017 27813-27814 HYPH denotes -
T7016 27814-27817 JJ denotes old
T7015 27810-27811 HYPH denotes -
T7014 27811-27813 NN denotes wk
T7013 27809-27810 CD denotes 4
T7012 27802-27803 SYM denotes −
T7011 27801-27802 HYPH denotes /
T7010 27800-27801 SYM denotes −
T7009 27796-27800 NN denotes MCAD
T7008 27789-27795 JJ denotes female
T7007 27804-27808 NNS denotes mice
T7006 27785-27788 CD denotes two
T7005 27781-27784 CC denotes and
T7004 27776-27780 JJ denotes male
T7003 27772-27775 CD denotes two
T7002 27770-27772 , denotes ,
T7001 27766-27767 HYPH denotes -
T7000 27767-27770 JJ denotes old
T6999 27763-27764 HYPH denotes -
T6998 27764-27766 NN denotes mo
T6997 27762-27763 CD denotes 6
T6996 27754-27755 SYM denotes +
T6995 27753-27754 HYPH denotes /
T6994 27752-27753 SYM denotes +
T6993 27748-27752 NN denotes MCAD
T6992 27741-27747 JJ denotes female
T6991 27756-27761 NN denotes mouse
T6990 27737-27740 CD denotes one
T6989 27733-27736 CC denotes and
T6988 27728-27732 JJ denotes male
T6987 27724-27727 CD denotes one
T6986 27722-27724 , denotes ,
T6985 27718-27719 HYPH denotes -
T6984 27719-27722 JJ denotes old
T6983 27715-27716 HYPH denotes -
T6982 27716-27718 NN denotes mo
T6981 27713-27715 CD denotes 18
T6980 27705-27706 SYM denotes −
T6979 27704-27705 HYPH denotes /
T6978 27703-27704 SYM denotes −
T6977 27699-27703 NN denotes MCAD
T6976 27692-27698 JJ denotes female
T6975 27707-27712 NN denotes mouse
T6974 27688-27691 CD denotes one
T6973 27684-27687 CC denotes and
T6972 27679-27683 JJ denotes male
T6971 27675-27678 CD denotes one
T6970 27665-27674 VBG denotes including
T6969 27663-27665 , denotes ,
T6968 27639-27649 JJ denotes histologic
T6967 27635-27638 CC denotes and
T6966 27650-27663 NNS denotes abnormalities
T6965 27629-27634 JJ denotes gross
T6964 27625-27628 IN denotes for
T6963 27611-27615 VBD denotes were
T6962 27616-27624 VBN denotes examined
T6961 27606-27610 NNS denotes mice
T6960 27599-27605 CD denotes Twelve
T6959 27598-27869 sentence denotes Twelve mice were examined for gross and histologic abnormalities, including one male and one female MCAD−/− mouse 18-mo-old, one male and one female MCAD+/+ mouse 6-mo-old, two male and two female MCAD−/− mice 4-wk-old, and two male and two female MCAD+/+ mice 4-wk-old.
T6958 27597-27598 . denotes .
T6957 27583-27597 NN denotes Histopathology
T6448 25890-25895 NNS denotes Blots
T6447 25889-26133 sentence denotes Blots were incubated in anti-mouse IgG HRP-conjugated secondary antibody for 2–4 h at room temperature using standard procedures and developed by chemiluminiscence (Renaissance, NEN Lifesciences Products, Boston, Massachusetts, United States).
T6446 25888-25889 . denotes .
T6445 25870-25879 JJ denotes anti-MCAD
T6444 25880-25888 NN denotes antibody
T6443 25867-25869 DT denotes an
T6442 25864-25866 IN denotes of
T6441 25855-25863 NN denotes dilution
T6440 25850-25851 SYM denotes :
T6439 25851-25854 CD denotes 500
T6438 25849-25850 CD denotes 1
T6437 25844-25848 IN denotes with
T6436 25841-25843 NN denotes °C
T6435 25839-25840 CD denotes 4
T6434 25836-25838 IN denotes at
T6433 25826-25835 RB denotes overnight
T6432 25808-25811 VBD denotes was
T6431 25799-25807 NN denotes membrane
T6430 25795-25798 DT denotes the
T6429 25793-25795 , denotes ,
T6428 25791-25793 CD denotes 20
T6427 25790-25791 HYPH denotes -
T6426 25785-25790 NN denotes Tween
T6425 25783-25784 NN denotes %
T6424 25780-25783 CD denotes 0.1
T6423 25775-25779 IN denotes with
T6359 25477-25478 -LRB- denotes (
T6358 25463-25470 NN denotes protein
T6357 25459-25462 NN denotes BCA
T6356 25471-25476 NN denotes assay
T6355 25450-25458 NNP denotes Bradford
T6354 25447-25449 IN denotes by
T6353 25431-25435 VBD denotes were
T6352 25436-25446 VBN denotes quantified
T6351 25423-25430 NNS denotes Lysates
T6350 25422-25524 sentence denotes Lysates were quantified by Bradford BCA protein assay (Bio-Rad, Hercules, California, United States).
T6349 25421-25422 . denotes .
T6348 25408-25421 NN denotes orthovanadate
T6347 25401-25407 NN denotes sodium
T6346 25398-25400 IN denotes of
T6345 25395-25397 NN denotes mM
T6344 25393-25394 CD denotes 1
T6343 25389-25392 CC denotes and
T6342 25387-25389 , denotes ,
T6341 25359-25387 NN denotes phenylmethylsulfonylfluoride
T6340 25356-25358 IN denotes of
T6339 25353-25355 NN denotes mM
T6338 25351-25352 CD denotes 1
T6337 25349-25351 , denotes ,
T6336 25348-25349 -RRB- denotes )
T6335 25342-25348 NNP denotes States
T6334 25335-25341 NNP denotes United
T6333 25333-25335 , denotes ,
T6332 25326-25333 NNP denotes Indiana
T6331 25324-25326 , denotes ,
T6330 25312-25324 NNP denotes Indianapolis
T6329 25310-25312 , denotes ,
T6328 25287-25298 NNP denotes Diagnostics
T6327 25281-25286 NNP denotes Roche
T6326 25299-25310 NNP denotes Corporation
T6325 25280-25281 -LRB- denotes (
T6324 25278-25280 , denotes ,
T6323 25260-25268 NN denotes Protease
T6322 25269-25278 NN denotes Inhibitor
T6321 25251-25259 JJ denotes Complete
T6320 25247-25250 CC denotes and
T6319 25238-25246 NN denotes glycerol
T6318 25236-25237 NN denotes %
T6317 25234-25236 CD denotes 10
T6316 25229-25233 IN denotes with
T6315 25227-25228 -RRB- denotes )
T6314 25224-25227 NN denotes SDS
T6313 25222-25223 NN denotes %
T6312 25221-25222 CD denotes 1
T6311 25217-25220 CC denotes and
T6310 25215-25217 , denotes ,
T6309 25196-25202 NN denotes sodium
T6308 25203-25215 NN denotes deoxycholate
T6307 25194-25195 NN denotes %
T6306 25191-25194 CD denotes 0.5
T6305 25189-25191 , denotes ,
T6304 25187-25189 CD denotes 40
T6303 25186-25187 HYPH denotes -
T6302 25177-25184 NN denotes Nonidet
T6301 25185-25186 NN denotes P
T6300 25175-25176 NN denotes %
T6299 25174-25175 CD denotes 1
T6298 25172-25174 , denotes ,
T6297 25167-25168 SYM denotes ×
T6296 25166-25167 CD denotes 1
T6295 25169-25172 NN denotes PBS
T6294 25165-25166 -LRB- denotes (
T6293 25158-25164 NN denotes buffer
T6292 25153-25157 NN denotes RIPA
T6291 25150-25152 IN denotes in
T6290 25144-25149 VBN denotes lysed
T6289 25140-25143 CC denotes and
T6288 25124-25127 VBD denotes was
T6287 25117-25123 NN denotes tissue
T6286 25115-25117 , denotes ,
T6285 25107-25115 NN denotes analysis
T6284 25128-25139 VBN denotes homogenized
T6283 25103-25106 IN denotes For
T6282 25102-25422 sentence denotes For analysis, tissue was homogenized and lysed in RIPA buffer (1× PBS, 1% Nonidet P-40, 0.5% sodium deoxycholate, and 1% SDS) with 10% glycerol and Complete Protease Inhibitor, (Roche Diagnostics Corporation, Indianapolis, Indiana, United States), 1 mM of phenylmethylsulfonylfluoride, and 1 mM of sodium orthovanadate.
T6281 25101-25102 . denotes .
T6280 25099-25101 NN denotes °C
T6279 25096-25098 CD denotes 80
T6278 25095-25096 SYM denotes −
T6277 25092-25094 IN denotes at
T6276 25085-25091 VBN denotes stored
T6275 25081-25084 CC denotes and
T6274 25072-25080 NN denotes nitrogen
T6273 25065-25071 JJ denotes liquid
T6272 25062-25064 IN denotes in
T6271 25043-25054 RB denotes immediately
T6270 25038-25042 VBD denotes were
T6269 25031-25032 -RRB- denotes )
T6268 25028-25029 SYM denotes =
T6267 25026-25027 NN denotes n
T6266 25030-25031 CD denotes 3
T6265 25025-25026 -LRB- denotes (
T6264 25023-25024 SYM denotes −
T6263 25022-25023 HYPH denotes /
T6262 25021-25022 SYM denotes −
T6261 25017-25021 NN denotes MCAD
T6260 25013-25016 CC denotes and
T6259 25011-25012 -RRB- denotes )
T6258 25008-25009 SYM denotes =
T6257 25006-25007 NN denotes n
T6256 25010-25011 CD denotes 1
T6255 25005-25006 -LRB- denotes (
T6254 25003-25004 SYM denotes +
T6253 25002-25003 HYPH denotes /
T6252 25001-25002 SYM denotes +
T6251 24997-25001 NN denotes MCAD
T6250 25033-25037 NNS denotes mice
T6249 24992-24993 HYPH denotes -
T6248 24993-24996 JJ denotes old
T6247 24989-24990 HYPH denotes -
T6246 24990-24992 NN denotes wk
T6245 24987-24988 SYM denotes –
T6244 24988-24989 CD denotes 8
T6243 24986-24987 CD denotes 6
T6242 24981-24985 IN denotes from
T6241 24973-24980 NNS denotes samples
T6240 24967-24972 NN denotes liver
T6239 24965-24967 , denotes ,
T6238 24959-24965 NN denotes tissue
T6237 24953-24958 NN denotes mouse
T6236 24950-24952 IN denotes in
T6235 24942-24949 NN denotes protein
T6234 24937-24941 NN denotes MCAD
T6233 24934-24936 IN denotes of
T6232 24925-24933 NN denotes quantity
T6231 24921-24924 DT denotes the
T6230 25055-25061 VBN denotes frozen
T6229 24912-24920 VB denotes evaluate
T6228 24909-24911 TO denotes To
T6227 24908-25102 sentence denotes To evaluate the quantity of MCAD protein in mouse tissue, liver samples from 6–8-wk-old MCAD+/+ (n = 1) and MCAD−/− (n = 3) mice were immediately frozen in liquid nitrogen and stored at −80 °C.
T6226 24907-24908 . denotes .
T6225 24900-24907 NN denotes protein
T6224 24895-24899 NN denotes MCAD
T6223 24892-24894 IN denotes of
T6222 24883-24891 NN denotes analysis
T6221 24872-24882 NN denotes Immunoblot
T6685 26481-26490 NN denotes challenge
T6735 26680-26840 sentence denotes Glucose concentration was measured in 10 μl sera using an Ektachem DT II system (Johnson and Johnson Clinical Diagnostics, Rochester, New York, United States).
T6734 26679-26680 . denotes .
T6733 26671-26679 NN denotes analysis
T6732 26668-26670 IN denotes to
T6731 26662-26667 JJ denotes prior
T6730 26660-26661 -RRB- denotes )
T6729 26639-26648 NN denotes carnitine
T6728 26635-26638 CC denotes and
T6727 26633-26635 , denotes ,
T6726 26629-26633 NN denotes acid
T6725 26621-26628 JJ denotes organic
T6724 26619-26621 , denotes ,
T6723 26609-26614 JJ denotes fatty
T6722 26615-26619 NN denotes acid
T6721 26604-26608 JJ denotes free
T6720 26602-26604 , denotes ,
T6719 26595-26602 NN denotes glucose
T6718 26589-26594 NN denotes serum
T6717 26649-26660 NNS denotes experiments
T6716 26588-26589 -LRB- denotes (
T6715 26586-26587 NN denotes h
T6714 26583-26585 CD denotes 24
T6713 26580-26582 CC denotes or
T6712 26578-26579 -RRB- denotes )
T6711 26557-26566 NN denotes tolerance
T6710 26552-26556 NN denotes cold
T6709 26567-26578 NNS denotes experiments
T6708 26551-26552 -LRB- denotes (
T6707 26549-26550 NN denotes h
T6706 26546-26548 CD denotes 18
T6705 26542-26545 IN denotes for
T6704 26530-26534 VBD denotes were
T6703 26535-26541 VBN denotes fasted
T6702 26523-26524 SYM denotes −
T6701 26522-26523 HYPH denotes /
T6700 26521-26522 SYM denotes −
T6699 26517-26521 NN denotes MCAD
T6698 26513-26516 CC denotes and
T6697 26511-26512 SYM denotes +
T6696 26510-26511 HYPH denotes /
T6695 26509-26510 SYM denotes +
T6694 26505-26509 NN denotes MCAD
T6693 26525-26529 NNS denotes mice
T6692 26500-26501 HYPH denotes -
T6691 26501-26504 JJ denotes old
T6690 26497-26498 HYPH denotes -
T6689 26498-26500 NN denotes wk
T6688 26492-26497 CD denotes Eight
T6687 26491-26680 sentence denotes Eight-wk-old MCAD+/+ and MCAD−/− mice were fasted for 18 h (cold tolerance experiments) or 24 h (serum glucose, free fatty acid, organic acid, and carnitine experiments) prior to analysis.
T6686 26490-26491 . denotes .
T6684 26476-26480 NN denotes cold
T6683 26472-26475 CC denotes and
T6682 26464-26471 NN denotes Fasting
T6642 26461-26462 . denotes .
T6641 26460-26461 -RRB- denotes ]
T6640 26458-26460 CD denotes 29
T6639 26457-26458 -LRB- denotes [
T6071 24869-24870 . denotes .
T6070 24858-24861 NN denotes RNA
T6069 24862-24869 NN denotes loading
T6068 24852-24857 JJ denotes equal
T6067 24845-24851 VB denotes verify
T6066 24842-24844 TO denotes to
T6065 24834-24841 NN denotes bromide
T6064 24825-24833 NN denotes ethidium
T6063 24822-24824 IN denotes by
T6062 24809-24813 VBD denotes were
T6061 24814-24821 VBN denotes stained
T6060 24790-24791 HYPH denotes -
T6059 24791-24803 NN denotes formaldehyde
T6058 24783-24790 NN denotes agarose
T6057 24804-24808 NNS denotes gels
T6056 24773-24782 JJ denotes Replicate
T6055 24772-24870 sentence denotes Replicate agarose-formaldehyde gels were stained by ethidium bromide to verify equal RNA loading.
T6054 24771-24772 . denotes .
T6053 24770-24771 -RRB- denotes )
T6052 24764-24770 NNP denotes States
T6051 24757-24763 NNP denotes United
T6050 24755-24757 , denotes ,
T6049 24749-24755 NNP denotes Jersey
T6048 24745-24748 NNP denotes New
T6047 24743-24745 , denotes ,
T6046 24733-24743 NNP denotes Piscataway
T6045 24731-24733 , denotes ,
T6044 24711-24719 NNP denotes Amersham
T6043 24700-24710 NNP denotes Healthcare
T6042 24697-24699 NNP denotes GE
T6041 24695-24696 : denotes ;
T6040 24693-24695 NNP denotes MP
T6039 24683-24692 NNP denotes Hyperfilm
T6038 24720-24731 NNP denotes Biosciences
T6037 24682-24683 -LRB- denotes (
T6036 24677-24681 NN denotes film
T6035 24660-24676 JJ denotes autoradiographic
T6034 24657-24659 IN denotes to
T6033 24645-24648 VBD denotes was
T6032 24649-24656 VBN denotes exposed
T6031 24638-24644 NN denotes filter
T6030 24634-24637 DT denotes The
T6029 24633-24772 sentence denotes The filter was exposed to autoradiographic film (Hyperfilm MP; GE Healthcare Amersham Biosciences, Piscataway, New Jersey, United States).
T6028 24632-24633 . denotes .
T6027 24631-24632 NN denotes h
T6026 24629-24630 CD denotes 1
T6025 24625-24628 IN denotes for
T6024 24622-24624 NNS denotes °C
T6023 24619-24621 CD denotes 55
T6022 24616-24618 IN denotes at
T6021 24612-24615 NN denotes SDS
T6020 24610-24611 NN denotes %
T6019 24607-24610 CD denotes 0.1
T6018 24605-24606 : denotes ;
T6017 24600-24601 SYM denotes ×
T6016 24602-24605 NN denotes SSC
T6015 24599-24600 CD denotes 2
T6014 24596-24598 IN denotes in
T6013 24590-24595 NNS denotes times
T6012 24586-24589 CD denotes two
T6011 24582-24585 CC denotes and
T6010 24578-24581 NN denotes SDS
T6009 24576-24577 NN denotes %
T6008 24573-24576 CD denotes 0.1
T6007 24571-24573 , denotes ,
T6006 24566-24567 SYM denotes ×
T6005 24568-24571 NN denotes SSC
T6004 24565-24566 CD denotes 4
T6003 24562-24564 IN denotes in
T6002 24556-24561 NNS denotes times
T6001 24552-24555 CD denotes two
T6000 24541-24544 VBD denotes was
T5999 24545-24551 VBN denotes washed
T5998 24523-24533 JJ denotes hybridized
T5997 24534-24540 NN denotes filter
T5996 24519-24522 DT denotes The
T5995 24518-24633 sentence denotes The hybridized filter was washed two times in 4× SSC, 0.1% SDS and two times in 2× SSC; 0.1% SDS at 55 °C for 1 h.
T5994 24517-24518 . denotes .
T5993 24516-24517 NN denotes h
T5992 24513-24515 CD denotes 18
T5991 24509-24512 IN denotes for
T5990 24507-24508 -RRB- denotes )
T5989 24504-24507 NN denotes DNA
T5988 24498-24503 NN denotes sperm
T5987 24491-24497 NN denotes salmon
T5986 24487-24490 CC denotes and
T5985 24485-24487 , denotes ,
T5984 24482-24485 NN denotes SDS
T5983 24480-24481 NN denotes %
T5982 24479-24480 CD denotes 1
T5981 24477-24479 , denotes ,
T5980 24467-24469 POS denotes 's
T5979 24459-24467 NNP denotes Denhardt
T5978 24457-24458 SYM denotes ×
T5977 24470-24477 NN denotes reagent
T5976 24456-24457 CD denotes 5
T5975 24454-24456 , denotes ,
T5974 24439-24446 NN denotes dextran
T5973 24447-24454 NN denotes sulfate
T5972 24437-24438 NN denotes %
T5971 24435-24437 CD denotes 10
T5970 24433-24435 , denotes ,
T5969 24424-24433 NN denotes formamide
T5968 24422-24423 NN denotes %
T5967 24420-24422 CD denotes 50
T5966 24418-24420 , denotes ,
T5965 24413-24414 SYM denotes ×
T5964 24415-24418 NN denotes SSC
T5963 24412-24413 CD denotes 2
T5962 24409-24411 IN denotes in
T5961 24403-24405 CD denotes 42
T5960 24406-24408 NN denotes °C
T5959 24402-24403 -LRB- denotes (
T5958 24391-24401 NNS denotes conditions
T5957 24381-24390 JJ denotes stringent
T5956 24374-24380 RB denotes highly
T5955 24368-24373 IN denotes under
T5954 24353-24357 VBD denotes were
T5953 24358-24367 VBN denotes performed
T5952 24338-24352 NNS denotes Hybridizations
T5951 24337-24518 sentence denotes Hybridizations were performed under highly stringent conditions (42 °C in 2× SSC, 50% formamide, 10% dextran sulfate, 5× Denhardt's reagent, 1% SDS, and salmon sperm DNA) for 18 h.
T5950 24336-24337 . denotes .
T5949 24335-24336 -RRB- denotes ]
T5948 24333-24335 CD denotes 28
T5947 24332-24333 -LRB- denotes [
T5946 24321-24331 NNS denotes procedures
T5945 24312-24320 JJ denotes standard
T5944 24306-24311 VBG denotes using
T5943 24295-24299 NN denotes cDNA
T5942 24289-24294 NN denotes Acadm
T5941 24283-24288 NN denotes mouse
T5940 24275-24276 HYPH denotes -
T5939 24276-24282 NN denotes length
T5938 24271-24275 JJ denotes full
T5937 24300-24305 NN denotes probe
T5936 24257-24258 HYPH denotes -
T5935 24258-24270 VBN denotes radiolabeled
T5934 24254-24257 NN denotes 32P
T5933 24249-24253 IN denotes with
T5932 24238-24248 VBN denotes hybridized
T5931 24234-24237 CC denotes and
T5930 24232-24234 , denotes ,
T5929 24231-24232 -RRB- denotes )
T5928 24225-24231 NNP denotes States
T5927 24218-24224 NNP denotes United
T5926 24216-24218 , denotes ,
T5925 24210-24216 NNP denotes Jersey
T6422 25768-25774 NN denotes saline
T6421 25758-25759 HYPH denotes -
T6420 25759-25767 VBN denotes buffered
T6419 25749-25758 NN denotes phosphate
T6418 25746-25748 IN denotes in
T6417 25734-25740 JJ denotes nonfat
T6416 25741-25745 NN denotes milk
T6415 25732-25733 NN denotes %
T6414 25731-25732 CD denotes 5
T6413 25726-25730 IN denotes with
T6412 25717-25725 VBG denotes blocking
T6411 25812-25825 VBN denotes immunoblotted
T6410 25711-25716 IN denotes After
T6409 25710-25889 sentence denotes After blocking with 5% nonfat milk in phosphate-buffered saline with 0.1% Tween-20, the membrane was immunoblotted overnight at 4 °C with 1:500 dilution of an anti-MCAD antibody.
T6408 25709-25710 . denotes .
T6407 25708-25709 -RRB- denotes )
T6406 25702-25708 NNP denotes States
T6405 25695-25701 NNP denotes United
T6404 25693-25695 , denotes ,
T6403 25684-25693 NNP denotes Hampshire
T6402 25680-25683 NNP denotes New
T6401 25678-25680 , denotes ,
T6400 25673-25678 NNP denotes Keene
T6399 25671-25673 , denotes ,
T6398 25664-25671 NNP denotes Schuell
T6397 25660-25663 CC denotes and
T6396 25649-25659 NNP denotes Schleicher
T6395 25648-25649 -LRB- denotes (
T6394 25624-25638 NN denotes nitrocellulose
T6393 25621-25623 NN denotes μm
T6392 25616-25620 CD denotes 0.45
T6391 25639-25647 NN denotes membrane
T6390 25614-25615 DT denotes a
T6389 25609-25613 IN denotes onto
T6388 25599-25608 RB denotes overnight
T6387 25587-25598 VBN denotes transferred
T6386 25583-25586 CC denotes and
T6385 25581-25583 , denotes ,
T6384 25576-25577 HYPH denotes -
T6383 25573-25576 NN denotes SDS
T6382 25577-25581 NN denotes PAGE
T6381 25571-25572 NN denotes %
T6380 25570-25571 CD denotes 8
T6379 25567-25569 IN denotes in
T6378 25557-25566 VBN denotes separated
T6377 25555-25557 , denotes ,
T6376 25541-25545 VBD denotes were
T6375 25546-25555 VBN denotes denatured
T6374 25533-25540 NNS denotes lysates
T6373 25525-25532 NN denotes Protein
T6372 25524-25710 sentence denotes Protein lysates were denatured, separated in 8% SDS-PAGE, and transferred overnight onto a 0.45 μm nitrocellulose membrane (Schleicher and Schuell, Keene, New Hampshire, United States).
T6371 25523-25524 . denotes .
T6370 25522-25523 -RRB- denotes )
T6369 25516-25522 NNP denotes States
T6368 25509-25515 NNP denotes United
T6367 25507-25509 , denotes ,
T6366 25497-25507 NNP denotes California
T6365 25495-25497 , denotes ,
T6364 25487-25495 NNP denotes Hercules
T6363 25485-25487 , denotes ,
T6362 25481-25482 HYPH denotes -
T6361 25478-25481 NNP denotes Bio
T6360 25482-25485 NNP denotes Rad
T5924 24206-24209 NNP denotes New
T5923 24204-24206 , denotes ,
T5922 24194-24204 NNP denotes Piscataway
T5921 24192-24194 , denotes ,
T5920 24175-24186 NNP denotes Biosciences
T5919 24166-24174 NNP denotes Amersham
T5918 24155-24165 NNP denotes Healthcare
T5917 24152-24154 NNP denotes GE
T5916 24150-24151 : denotes ;
T5915 24149-24150 NNP denotes N
T5914 24142-24148 NNP denotes Hybond
T5913 24187-24192 NNP denotes Corp.
T5912 24141-24142 -LRB- denotes (
T5911 24134-24140 NN denotes filter
T5910 24119-24133 NN denotes nitrocellulose
T5909 24116-24118 IN denotes to
T5908 24104-24115 VBN denotes transferred
T5907 24102-24104 , denotes ,
T5906 24085-24086 HYPH denotes -
T5905 24078-24085 NN denotes agarose
T5904 24086-24098 NN denotes formaldehyde
T5903 24076-24077 NN denotes %
T5902 24073-24076 CD denotes 0.8
T5901 24099-24102 NN denotes gel
T5900 24071-24072 DT denotes a
T5899 24066-24070 IN denotes onto
T5891 24059-24065 VBN denotes loaded
T5890 24022-24024 NN denotes μg
T5889 24018-24021 CD denotes Ten
T5888 24017-24337 sentence denotes Ten μg of total RNA from each sample was loaded onto a 0.8% agarose-formaldehyde gel, transferred to nitrocellulose filter (Hybond N; GE Healthcare Amersham Biosciences Corp., Piscataway, New Jersey, United States), and hybridized with 32P-radiolabeled full-length mouse Acadm cDNA probe using standard procedures [28].
T5887 24016-24017 . denotes .
T5886 24005-24007 POS denotes 's
T5885 24008-24016 NN denotes protocol
T5884 23993-24005 NN denotes manufacturer
T5883 23989-23992 IN denotes per
T5882 23986-23988 IN denotes as
T5881 23984-23985 -RRB- denotes )
T5880 23978-23984 NNP denotes States
T5879 23971-23977 NNP denotes United
T5878 23969-23971 , denotes ,
T5877 23964-23969 NNP denotes Texas
T5876 23962-23964 , denotes ,
T5875 23955-23962 NNP denotes Houston
T5874 23953-23955 , denotes ,
T5873 23949-23953 NNP denotes Inc.
T5872 23947-23949 , denotes ,
T5871 23928-23934 NNP denotes BIOTEX
T5870 23935-23947 NNP denotes Laboratories
T5869 23927-23928 -LRB- denotes (
T5868 23913-23922 NN denotes Isolation
T5867 23909-23912 NN denotes RNA
T5866 23899-23908 NNP denotes Ultraspec
T5865 23923-23926 NN denotes Kit
T5864 23895-23898 DT denotes the
T5863 23889-23894 VBG denotes using
T5862 23882-23883 SYM denotes −
T5861 23881-23882 HYPH denotes /
T5860 23880-23881 SYM denotes −
T5859 23876-23880 NN denotes MCAD
T5858 23872-23875 CC denotes and
T5857 23870-23871 SYM denotes +
T5856 23869-23870 HYPH denotes /
T5855 23868-23869 SYM denotes +
T5854 23864-23868 NN denotes MCAD
T5853 23884-23888 NNS denotes mice
T5852 23859-23860 HYPH denotes -
T5851 23860-23863 JJ denotes old
T5850 23856-23857 HYPH denotes -
T5849 23857-23859 NN denotes mo
T5848 23855-23856 CD denotes 3
T5847 23852-23854 IN denotes of
T5846 23845-23851 NNS denotes testes
T5845 23841-23844 CC denotes and
T5844 23839-23841 , denotes ,
T5843 23836-23839 NN denotes fat
T5842 23830-23835 JJ denotes white
T5841 23828-23830 , denotes ,
T5840 23822-23828 NN denotes muscle
T5839 23813-23821 JJ denotes skeletal
T5838 23811-23813 , denotes ,
T5837 23805-23811 NN denotes kidney
T5836 23803-23805 , denotes ,
T5835 23798-23803 NN denotes brain
T5834 23796-23798 , denotes ,
T5833 23793-23796 NN denotes fat
T5832 23787-23792 JJ denotes brown
T5831 23785-23787 , denotes ,
T5830 23780-23785 NN denotes liver
T5829 23778-23780 , denotes ,
T5828 23773-23778 NN denotes heart
T5827 23768-23772 IN denotes from
T5826 23755-23758 VBD denotes was
T5825 23759-23767 VBN denotes isolated
T5824 23751-23754 NN denotes RNA
T5823 23745-23750 JJ denotes Total
T5822 23744-24017 sentence denotes Total RNA was isolated from heart, liver, brown fat, brain, kidney, skeletal muscle, white fat, and testes of 3-mo-old MCAD+/+ and MCAD−/− mice using the Ultraspec RNA Isolation Kit (BIOTEX Laboratories, Inc., Houston, Texas, United States) as per manufacturer's protocol.
T5821 23743-23744 . denotes .
T5820 23730-23733 VBD denotes was
T5819 23721-23729 NN denotes analysis
T5818 23716-23720 NN denotes blot
T5817 23707-23715 NNP denotes northern
T5816 23705-23707 , denotes ,
T5815 23699-23700 SYM denotes −
T5814 23698-23699 HYPH denotes /
T5813 23697-23698 SYM denotes −
T5812 23693-23697 NN denotes MCAD
T5811 23701-23705 NNS denotes mice
T5810 23689-23692 DT denotes the
T5809 23684-23688 IN denotes from
T5808 23674-23683 VBN denotes expressed
T5807 23669-23673 NN denotes mRNA
T5806 23663-23668 NN denotes Acadm
T5805 23660-23662 IN denotes of
T5804 23653-23659 NN denotes extent
T5803 23649-23652 DT denotes the
T5802 23639-23648 VB denotes determine
T5801 23636-23638 TO denotes to
T5800 23630-23635 NN denotes order
T5799 23734-23743 VBN denotes performed
T5798 23627-23629 IN denotes In
T5797 23626-23744 sentence denotes In order to determine the extent of Acadm mRNA expressed from the MCAD−/− mice, northern blot analysis was performed.
T5796 23625-23626 . denotes .
T5795 23624-23625 -RRB- denotes )
T5794 23618-23624 NNP denotes States
T5793 23611-23617 NNP denotes United
T5792 23609-23611 , denotes ,
T5791 23600-23609 NNP denotes Wisconsin
T5790 23598-23600 , denotes ,
T5789 23591-23598 NNP denotes Madison
T5788 23589-23591 , denotes ,
T5787 23582-23589 NNP denotes Promega
T5786 23581-23582 -LRB- denotes (
T5785 23569-23573 NNP denotes Easy
T5784 23574-23580 NNP denotes vector
T5783 23566-23567 HYPH denotes -
T5782 23567-23568 NN denotes T
T5781 23562-23566 NN denotes pGEM
T5780 23557-23561 IN denotes into
T5779 23546-23556 VBG denotes subcloning
T5778 23540-23545 IN denotes after
T5777 23517-23529 RB denotes subsequently
T5776 23512-23516 VBD denotes were
T5775 23530-23539 VBN denotes sequenced
T5774 23502-23511 NNS denotes fragments
T5773 23498-23501 NN denotes PCR
T5772 23497-23626 sentence denotes PCR fragments were subsequently sequenced after subcloning into pGEM-T Easy vector (Promega, Madison, Wisconsin, United States).
T5771 23496-23497 . denotes .
T5770 23491-23496 RB denotes above
T5769 23481-23490 VBN denotes described
T5768 23478-23480 IN denotes as
T5767 23463-23467 VBD denotes were
T5766 23468-23477 VBN denotes performed
T5765 23448-23462 NNS denotes amplifications
T5764 23444-23447 NN denotes PCR
T5763 23443-23497 sentence denotes PCR amplifications were performed as described above.
T5762 23442-23443 . denotes .
T5761 23437-23442 NN denotes Acadm
T5760 23434-23436 IN denotes of
T5759 23431-23433 CD denotes 11
T5758 23426-23430 NN denotes exon
T5757 23422-23425 CC denotes and
T5756 23420-23421 CD denotes 7
T5755 23415-23419 NN denotes exon
T5754 23412-23414 IN denotes to
T5753 23403-23411 JJ denotes specific
T5752 23386-23402 NNS denotes oligonucleotides
T5751 23380-23385 VBG denotes using
T5750 23357-23369 RB denotes subsequently
T5749 23353-23356 VBD denotes was
T5748 23370-23379 VBN denotes performed
T5747 23349-23352 NN denotes PCR
T5746 23348-23443 sentence denotes PCR was subsequently performed using oligonucleotides specific to exon 7 and exon 11 of Acadm.
T5745 23347-23348 . denotes .
T5744 23346-23347 -RRB- denotes )
T5743 23340-23346 NNP denotes States
T5742 23333-23339 NNP denotes United
T5741 23331-23333 , denotes ,
T5740 23321-23331 NNP denotes California
T5739 23319-23321 , denotes ,
T5738 23315-23319 NNP denotes View
T5737 23306-23314 NNP denotes Mountain
T5736 23304-23306 , denotes ,
T5735 23296-23304 NNP denotes Clontech
T5734 23295-23296 -LRB- denotes (
T5733 23282-23294 NN denotes manufacturer
T5732 23278-23281 DT denotes the
T5731 23275-23277 IN denotes by
T5730 23263-23274 VBN denotes recommended
T5729 23260-23262 IN denotes as
T5728 23243-23259 NNS denotes oligonucleotides
T5727 23236-23242 JJ denotes random
T5726 23230-23235 VBG denotes using
T5725 23216-23219 VBD denotes was
T5724 23220-23229 VBN denotes performed
T5723 23202-23215 NN denotes transcription
T5722 23194-23201 JJ denotes Reverse
T5721 23193-23348 sentence denotes Reverse transcription was performed using random oligonucleotides as recommended by the manufacturer (Clontech, Mountain View, California, United States).
T5720 23192-23193 . denotes .
T5719 23191-23192 -RRB- denotes ]
T5718 23189-23191 CD denotes 27
T5717 23188-23189 -LRB- denotes [
T5716 23169-23180 NN denotes thiocyanate
T5715 23181-23187 NN denotes method
T5714 23157-23168 NN denotes guanidinium
T5713 23151-23156 VBG denotes using
T5712 23140-23150 NNS denotes techniques
T5711 23131-23139 JJ denotes standard
T5710 23128-23130 IN denotes by
T5709 23121-23122 SYM denotes −
T5708 23120-23121 HYPH denotes /
T5707 23119-23120 SYM denotes −
T5706 23115-23119 NN denotes MCAD
T5705 23111-23114 CC denotes and
T5704 23109-23111 , denotes ,
T5703 23108-23109 SYM denotes −
T5702 23107-23108 HYPH denotes /
T5701 23106-23107 SYM denotes +
T5700 23102-23106 NN denotes MCAD
T5699 23100-23102 , denotes ,
T5698 23099-23100 SYM denotes +
T5697 23098-23099 HYPH denotes /
T5696 23097-23098 SYM denotes +
T5695 23093-23097 NN denotes MCAD
T5694 23123-23127 NNS denotes mice
T5693 23089-23092 JJ denotes old
T5692 23085-23088 NN denotes day
T5691 23082-23084 CD denotes 30
T5690 23079-23081 IN denotes of
T5689 23073-23078 NN denotes heart
T5688 23069-23072 DT denotes the
T5687 23064-23068 IN denotes from
T5686 23051-23054 VBD denotes was
T5685 23055-23063 VBN denotes isolated
T5684 23047-23050 NN denotes RNA
T5683 23041-23046 JJ denotes Total
T5682 23040-23193 sentence denotes Total RNA was isolated from the heart of 30 day old MCAD+/+, MCAD+/−, and MCAD−/− mice by standard techniques using guanidinium thiocyanate method [27].
T5681 23039-23040 . denotes .
T5680 23031-23039 NN denotes analysis
T5679 23027-23030 NN denotes RNA
T5536 23024-23025 . denotes .
T5535 23012-23017 JJ denotes major
T5534 23018-23024 NNS denotes organs
T5533 23008-23011 DT denotes all
T5532 23005-23007 IN denotes of
T5531 22990-23004 NN denotes histopathology
T5530 22986-22989 CC denotes and
T5529 22984-22986 , denotes ,
T5528 22967-22970 CC denotes and
T5527 22965-22966 HYPH denotes -
T5526 22971-22984 NNS denotes ectoparasites
T5525 22961-22965 AFX denotes endo
T5524 22957-22960 IN denotes for
T5523 22944-22956 NNS denotes examinations
T5522 22942-22944 , denotes ,
T5521 22937-22942 NN denotes cecum
T5520 22933-22936 CC denotes and
T5519 22921-22932 NN denotes nasopharynx
T5518 22918-22920 IN denotes of
T5517 22899-22908 JJ denotes bacterial
T5516 22909-22917 NNS denotes cultures
T5515 22891-22898 JJ denotes aerobic
T5514 22889-22891 , denotes ,
T5513 22872-22881 JJ denotes different
T5512 22882-22889 NNS denotes viruses
T5511 22868-22871 CD denotes ten
T5510 22864-22867 IN denotes for
T5509 22857-22863 NNS denotes assays
T5508 22845-22856 JJ denotes serological
T5507 22842-22844 IN denotes on
T5506 22836-22841 VBN denotes based
T5505 22826-22835 NNS denotes pathogens
T5504 22820-22825 NN denotes mouse
T5503 22816-22819 IN denotes for
T5502 22807-22815 JJ denotes negative
T5501 22802-22806 VBD denotes were
T5500 22797-22801 NNS denotes Mice
T5499 22796-23025 sentence denotes Mice were negative for mouse pathogens based on serological assays for ten different viruses, aerobic bacterial cultures of nasopharynx and cecum, examinations for endo- and ectoparasites, and histopathology of all major organs.
T5498 22795-22796 . denotes .
T5497 22789-22790 -RRB- denotes )
T5496 22786-22789 CD denotes 129
T5495 22785-22786 : denotes ;
T5494 22783-22785 NN denotes B6
T5493 22782-22783 -LRB- denotes (
T5492 22780-22781 SYM denotes −
T5491 22779-22780 HYPH denotes /
T5490 22778-22779 SYM denotes −
T5489 22774-22778 NN denotes MCAD
T5488 22766-22767 HYPH denotes /
T5487 22767-22773 NN denotes tm1Uab
T5486 22755-22766 NN denotes AcadmtmUab1
T5485 22791-22795 NNS denotes mice
T5484 22744-22754 RB denotes eventually
T5483 22740-22743 CC denotes and
T5482 22738-22739 -RRB- denotes )
T5481 22737-22738 SYM denotes −
T5480 22736-22737 HYPH denotes /
T5479 22735-22736 SYM denotes +
T5478 22731-22735 NN denotes MCAD
T5477 22730-22731 -LRB- denotes (
T5476 22728-22729 SYM denotes +
T5475 22727-22728 HYPH denotes /
T5474 22716-22727 NN denotes AcadmtmUab1
T5473 22711-22715 NNS denotes mice
T5472 22701-22702 HYPH denotes -
T5471 22702-22710 VBN denotes targeted
T5470 22697-22701 NN denotes gene
T5469 22689-22696 VB denotes produce
T5468 22686-22688 TO denotes to
T5467 22675-22680 NN denotes 129P2
T5466 22671-22674 CC denotes and
T5465 22681-22685 NNS denotes mice
T5464 22668-22670 NN denotes B6
T5463 22665-22667 IN denotes to
T5462 22640-22652 RB denotes subsequently
T5461 22635-22639 VBD denotes were
T5460 22653-22664 VBN denotes backcrossed
T5459 22630-22634 NNS denotes mice
T5458 22621-22629 JJ denotes Chimeric
T5457 22620-22796 sentence denotes Chimeric mice were subsequently backcrossed to B6 and 129P2 mice to produce gene-targeted mice AcadmtmUab1/+ (MCAD+/−) and eventually AcadmtmUab1/tm1Uab MCAD−/− (B6;129) mice.
T5456 22619-22620 . denotes .
T5455 22615-22619 NNS denotes mice
T5454 22606-22614 JJ denotes chimeric
T5453 22597-22605 VB denotes generate
T5452 22594-22596 TO denotes to
T5451 22582-22593 NNS denotes blastocysts
T5450 22579-22581 NN denotes B6
T5449 22574-22578 IN denotes into
T5448 22555-22559 VBD denotes were
T5447 22560-22573 VBN denotes microinjected
T5446 22543-22547 NN denotes cell
T5445 22540-22542 NN denotes ES
T5444 22548-22554 NNS denotes clones
T5443 22531-22539 VBN denotes targeted
T5442 22521-22530 RB denotes Correctly
T5441 22520-22620 sentence denotes Correctly targeted ES cell clones were microinjected into B6 blastocysts to generate chimeric mice.
T5440 22519-22520 . denotes .
T5439 22506-22519 NN denotes recombination
T5438 22495-22505 JJ denotes homologous
T5898 24055-24058 VBD denotes was
T5897 24048-24054 NN denotes sample
T5896 24043-24047 DT denotes each
T5895 24038-24042 IN denotes from
T5894 24034-24037 NN denotes RNA
T5893 24028-24033 JJ denotes total
T5892 24025-24027 IN denotes of
T5437 22490-22494 IN denotes upon
T5436 22477-22480 NN denotes DNA
T5435 22469-22476 JJ denotes genomic
T5434 22465-22466 HYPH denotes -
T5433 22466-22468 NN denotes kb
T5432 22461-22465 CD denotes 13.2
T5431 22481-22489 NN denotes fragment
T5430 22459-22460 DT denotes a
T5429 22456-22458 IN denotes to
T5428 22446-22455 VB denotes hybridize
T5427 22443-22445 TO denotes to
T5426 22434-22442 VBN denotes expected
T5425 22430-22433 VBD denotes was
T5424 22426-22429 CC denotes and
T5423 22409-22418 NN denotes insertion
T5422 22419-22425 NN denotes vector
T5421 22403-22408 NN denotes Acadm
T5420 22396-22402 IN denotes within
T5419 22388-22395 JJ denotes present
T5418 22384-22387 RB denotes not
T5417 22381-22383 VBZ denotes is
T5416 22368-22371 NN denotes DNA
T5415 22372-22380 NN denotes fragment
T5414 22363-22367 DT denotes This
T5413 22362-22520 sentence denotes This DNA fragment is not present within Acadm insertion vector and was expected to hybridize to a 13.2-kb genomic DNA fragment upon homologous recombination.
T5412 22361-22362 . denotes .
T5397 22293-22294 HYPH denotes -
T5396 22294-22296 NN denotes bp
T5395 22290-22293 CD denotes 850
T5394 22297-22302 NN denotes probe
T5393 22287-22289 DT denotes an
T5392 22282-22286 IN denotes with
T5391 22275-22281 VBN denotes probed
T5390 22271-22274 CC denotes and
T5389 22269-22271 , denotes ,
T5388 22262-22269 VBN denotes blotted
T5387 22260-22262 , denotes ,
T5386 22245-22260 VBN denotes electrophoresed
T5385 22243-22245 , denotes ,
T5384 22238-22243 NN denotes EcoRI
T5383 22233-22237 IN denotes with
T5382 22220-22223 VBD denotes was
T5381 22208-22210 NN denotes ES
T5380 22211-22219 NNS denotes colonies
T5379 22198-22207 JJ denotes resistant
T5378 22193-22197 NN denotes G418
T5377 22188-22192 IN denotes from
T5376 22224-22232 VBN denotes digested
T5375 22184-22187 NN denotes DNA
T5374 22176-22183 JJ denotes Genomic
T5373 22175-22362 sentence denotes Genomic DNA from G418 resistant ES colonies was digested with EcoRI, electrophoresed, blotted, and probed with an 850-bp probe (probe A) generated by PCR from Acadm exon 10 to intron 10.
T5372 22174-22175 . denotes .
T5371 22161-22174 NN denotes recombination
T5370 22150-22160 JJ denotes homologous
T5369 22142-22149 VB denotes confirm
T5368 22139-22141 TO denotes to
T5367 22125-22128 VBD denotes was
T5366 22129-22138 VBN denotes conducted
T5365 22116-22124 NN denotes analysis
T5364 22111-22115 NN denotes blot
T5363 22102-22110 NNP denotes Southern
T5362 22101-22175 sentence denotes Southern blot analysis was conducted to confirm homologous recombination.
T5361 22100-22101 . denotes .
T5360 22087-22100 NN denotes recombination
T5359 22076-22086 JJ denotes homologous
T5358 22069-22075 IN denotes during
T5357 22067-22068 -RRB- denotes ]
T5356 22065-22067 CD denotes 26
T5355 22064-22065 -LRB- denotes [
T5354 22057-22063 NN denotes repair
T5353 22050-22051 HYPH denotes –
T5352 22051-22056 NN denotes break
T5351 22042-22050 VBN denotes stranded
T5350 22035-22041 JJ denotes double
T5349 22030-22034 IN denotes upon
T5348 22017-22018 HYPH denotes -
T5347 22018-22020 NN denotes kb
T5346 22014-22017 CD denotes 1.3
T5345 22021-22029 NN denotes deletion
T5344 22010-22013 DT denotes the
T5343 22007-22009 IN denotes of
T5342 22000-22006 NN denotes repair
T5341 21996-21999 NN denotes gap
T5340 21988-21995 VB denotes undergo
T5339 21985-21987 TO denotes to
T5338 21972-21975 VBD denotes was
T5337 21976-21984 VBN denotes designed
T5336 21955-21964 NN denotes insertion
T5335 21949-21954 NN denotes Acadm
T5334 21965-21971 NN denotes vector
T5333 21940-21948 VBN denotes targeted
T5332 21930-21939 RB denotes Correctly
T5331 21929-22101 sentence denotes Correctly targeted Acadm insertion vector was designed to undergo gap repair of the 1.3-kb deletion upon double stranded–break repair [26] during homologous recombination.
T5330 21928-21929 . denotes .
T5329 21924-21928 NNS denotes mice
T5328 21918-21923 NN denotes 129P2
T5327 21913-21917 IN denotes from
T5326 21905-21912 VBN denotes derived
T5325 21903-21905 , denotes ,
T5324 21902-21903 -RRB- denotes )
T5323 21898-21902 NNP denotes Kuhn
T5322 21895-21897 NNP denotes R.
T5321 21890-21894 IN denotes from
T5320 21880-21884 JJ denotes kind
T5319 21878-21879 DT denotes a
T5318 21885-21889 NN denotes gift
T5317 21877-21878 -LRB- denotes (
T5316 21868-21870 NN denotes ES
T5315 21866-21867 CD denotes 1
T5314 21865-21866 HYPH denotes –
T5313 21871-21876 NNS denotes cells
T5312 21862-21865 NN denotes E14
T5311 21857-21861 IN denotes into
T5310 21842-21856 VBN denotes electroporated
T5309 21838-21841 CC denotes and
T5308 21836-21838 , denotes ,
T5307 21819-21827 NN denotes fragment
T5306 21811-21818 JJ denotes genomic
T5305 21807-21808 HYPH denotes -
T5304 21808-21810 NN denotes kb
T5303 21804-21807 CD denotes 1.3
T5302 21828-21836 NN denotes deletion
T5301 21800-21803 DT denotes the
T5300 21797-21799 IN denotes of
T5299 21792-21796 NN denotes site
T5298 21788-21791 DT denotes the
T5297 21786-21788 , denotes ,
T5296 21777-21786 NN denotes digestion
T5295 21772-21776 NN denotes SalI
T5294 21769-21771 IN denotes by
T5293 21754-21757 VBD denotes was
T5292 21758-21768 VBN denotes linearized
T5291 21737-21746 NN denotes insertion
T5290 21731-21736 NN denotes Acadm
T5289 21747-21753 NN denotes vector
T5288 21727-21730 DT denotes The
T5287 21726-21929 sentence denotes The Acadm insertion vector was linearized by SalI digestion, the site of the 1.3-kb genomic fragment deletion, and electroporated into E14–1 ES cells (a kind gift from R. Kuhn), derived from 129P2 mice.
T5286 21725-21726 . denotes .
T5285 21721-21725 NNS denotes mice
T5284 21710-21711 HYPH denotes -
T5283 21711-21720 JJ denotes deficient
T5282 21706-21710 NN denotes MCAD
T5281 21703-21705 IN denotes of
T5280 21692-21702 NN denotes Generation
T5173 21663-21667 NN denotes exon
T5172 21659-21660 HYPH denotes -
T5171 21660-21662 NN denotes kb
T5170 21656-21659 CD denotes 1.3
T5169 21671-21677 NN denotes region
T5168 21652-21655 DT denotes the
T5167 21682-21689 VBN denotes deleted
T5166 21646-21651 WRB denotes where
T5165 21636-21640 NN denotes SalI
T5164 21629-21635 JJ denotes unique
T5163 21641-21645 NN denotes site
T5162 21627-21628 DT denotes a
T5161 21615-21617 , denotes ,
T5160 21607-21615 NN denotes homology
T5159 21604-21606 IN denotes of
T5158 21594-21599 JJ denotes short
T5157 21600-21603 NN denotes arm
T5156 21590-21593 DT denotes the
T5155 21587-21589 IN denotes to
T5154 21578-21582 JJ denotes long
T5153 21583-21586 NN denotes arm
T5152 21574-21577 DT denotes the
T5151 21571-21573 IN denotes of
T5150 21562-21570 NN denotes ligation
T5149 21558-21561 DT denotes the
T5148 21553-21557 IN denotes from
T5147 21543-21552 VBG denotes resulting
T5146 21541-21543 , denotes ,
T5145 21617-21626 VBD denotes contained
T5144 21520-21534 VBN denotes recircularized
T5143 21535-21541 NN denotes vector
T5142 21516-21519 DT denotes The
T5141 21515-21690 sentence denotes The recircularized vector, resulting from the ligation of the long arm to the short arm of homology, contained a unique SalI site where the 1.3-kb exon 10 region was deleted.
T5140 21514-21515 . denotes .
T5139 21513-21514 SYM denotes ′
T5138 21512-21513 CD denotes 3
T5137 21511-21512 HYPH denotes -
T5136 21499-21500 HYPH denotes -
T5135 21498-21499 SYM denotes ′
T5134 21500-21511 NN denotes TCGATGTCGAC
T5133 21497-21498 CD denotes 5
T5132 21493-21496 CC denotes and
T5131 21491-21492 : denotes ;
T5130 21490-21491 SYM denotes ′
T5129 21489-21490 CD denotes 3
T5128 21488-21489 HYPH denotes -
T5127 21476-21477 SYM denotes ′
T5126 21477-21488 NN denotes GATCGTCGACA
T5125 21475-21476 CD denotes 5
T5124 21473-21474 : denotes ;
T5123 21472-21473 SYM denotes ′
T5122 21471-21472 CD denotes 3
T5121 21470-21471 HYPH denotes -
T5120 21458-21459 HYPH denotes -
T5119 21457-21458 SYM denotes ′
T5118 21459-21470 NN denotes AATTGTCGACA
T5117 21456-21457 CD denotes 5
T5116 21454-21456 : denotes :
T5115 21438-21454 NNS denotes oligonucleotides
T5114 21432-21437 CD denotes three
T5113 21426-21431 VBG denotes using
T5112 21417-21425 NN denotes ligation
T5111 21414-21416 IN denotes by
T5110 21399-21413 VBN denotes recircularized
T5109 21395-21398 CC denotes and
T5108 21382-21394 NN denotes purification
T5107 21378-21381 NN denotes gel
T5106 21375-21377 IN denotes by
T5105 21362-21365 VBD denotes was
T5104 21360-21362 , denotes ,
T5103 21344-21351 JJ denotes genomic
T5102 21337-21338 HYPH denotes /
T5101 21338-21343 NN denotes EcoRI
T5100 21332-21337 NN denotes BamHI
T5099 21328-21329 HYPH denotes -
T5098 21329-21331 NN denotes kb
T5097 21325-21328 CD denotes 1.3
T5096 21352-21360 NN denotes fragment
T5095 21321-21324 DT denotes the
T5094 21313-21320 IN denotes without
T5093 21311-21313 , denotes ,
T5092 21366-21374 VBN denotes purified
T5091 21296-21304 VBN denotes digested
T5090 21305-21311 NN denotes vector
T5089 21292-21295 DT denotes The
T5088 21291-21515 sentence denotes The digested vector, without the 1.3-kb BamHI/EcoRI genomic fragment, was purified by gel purification and recircularized by ligation using three oligonucleotides: 5′-AATTGTCGACA-3′; 5′GATCGTCGACA-3′; and 5′-TCGATGTCGAC-3′.
T5087 21290-21291 . denotes .
T5086 21281-21290 NNS denotes sequences
T5085 21274-21280 NN denotes intron
T5084 21265-21273 VBG denotes flanking
T5083 21261-21264 CC denotes and
T5082 21258-21260 CD denotes 10
T5081 21253-21257 NN denotes exon
T5080 21242-21252 VBG denotes containing
T5079 21225-21232 JJ denotes genomic
T5078 21218-21219 HYPH denotes /
T5077 21219-21224 NN denotes EcoRI
T5076 21213-21218 NN denotes BamHI
T5075 21209-21210 HYPH denotes -
T5074 21210-21212 NN denotes kb
T5073 21206-21209 CD denotes 1.3
T5072 21233-21241 NN denotes fragment
T5071 21204-21205 DT denotes a
T5070 21197-21203 VB denotes remove
T5069 21194-21196 TO denotes to
T5068 21188-21193 NN denotes EcoRI
T5067 21184-21187 CC denotes and
T5066 21178-21183 NN denotes BamHI
T5065 21173-21177 IN denotes with
T5064 21160-21163 VBD denotes was
T5063 21164-21172 VBN denotes digested
T5062 21153-21159 NN denotes vector
T5061 21149-21152 DT denotes The
T5060 21148-21291 sentence denotes The vector was digested with BamHI and EcoRI to remove a 1.3-kb BamHI/EcoRI genomic fragment containing exon 10 and flanking intron sequences.
T5059 21147-21148 . denotes .
T5058 21146-21147 -RRB- denotes )
T5057 21145-21146 SYM denotes +
T5056 21144-21145 -LRB- denotes (
T5055 21136-21144 NN denotes pGEM11Zf
T5054 21133-21135 IN denotes of
T5053 21122-21126 NN denotes XhoI
T5052 21118-21121 CC denotes and
T5051 21113-21117 NN denotes NotI
T5050 21127-21132 NNS denotes sites
T5049 21109-21112 DT denotes the
T5048 21104-21108 IN denotes into
T5047 21083-21096 RB denotes directionally
T5046 21079-21082 VBD denotes was
T5045 21077-21079 , denotes ,
T5044 21076-21077 -RRB- denotes ]
T5043 21073-21074 , denotes ,
T5042 21071-21073 CD denotes 24
T5041 21074-21076 CD denotes 25
T5040 21070-21071 -LRB- denotes [
T5039 21054-21061 JJ denotes genomic
T5038 21048-21053 NN denotes mouse
T5037 21042-21047 NN denotes 129Sv
T5036 21036-21041 NN denotes FIXII
T5035 21029-21035 NNP denotes Lambda
T5034 21062-21069 NN denotes library
T5033 21027-21028 DT denotes a
T5032 21022-21026 IN denotes from
T5031 21013-21021 VBN denotes obtained
T5030 21002-21012 RB denotes originally
T5029 21000-21002 , denotes ,
T5028 20991-21000 NNS denotes sequences
T5027 20984-20990 NN denotes intron
T5026 20975-20983 VBG denotes flanking
T5025 20971-20974 CC denotes and
T5024 20968-20970 CD denotes 10
T5023 20964-20967 CC denotes and
T5022 20962-20964 , denotes ,
T5021 20961-20962 CD denotes 9
T5020 20959-20961 , denotes ,
T5019 20958-20959 CD denotes 8
T5018 20952-20957 NNS denotes exons
T5017 20943-20951 VBG denotes spanning
T5016 20926-20933 JJ denotes genomic
T5015 20920-20925 NN denotes Acadm
T5014 20916-20917 HYPH denotes -
T5013 20917-20919 NN denotes kb
T5012 20915-20916 CD denotes 8
T5011 20934-20942 NN denotes fragment
T5411 22359-22361 CD denotes 10
T5410 22352-22358 NN denotes intron
T5409 22349-22351 IN denotes to
T5408 22346-22348 CD denotes 10
T5407 22341-22345 NN denotes exon
T5406 22335-22340 NN denotes Acadm
T5405 22330-22334 IN denotes from
T5404 22326-22329 NN denotes PCR
T5403 22323-22325 IN denotes by
T5402 22313-22322 VBN denotes generated
T5401 22311-22312 -RRB- denotes )
T5400 22304-22309 NN denotes probe
T5399 22310-22311 NN denotes A
T5398 22303-22304 -LRB- denotes (
T4974 20736-20742 VBN denotes filled
T4973 20721-20730 NNS denotes overhangs
T4972 20717-20720 DT denotes the
T4971 20713-20716 CC denotes and
T4970 20707-20712 NN denotes EcoRI
T4969 20702-20706 IN denotes with
T4968 20689-20692 VBD denotes was
T4967 20693-20701 VBN denotes digested
T4966 20681-20688 NN denotes plasmid
T4965 20677-20680 DT denotes The
T4964 20676-20762 sentence denotes The plasmid was digested with EcoRI and the overhangs were filled with Klenow enzyme.
T4963 20675-20676 . denotes .
T4962 20674-20675 -RRB- denotes )
T4961 20673-20674 SYM denotes +
T4960 20672-20673 -LRB- denotes (
T4959 20664-20672 NN denotes pGEM11Zf
T4958 20661-20663 IN denotes of
T5176 21689-21690 . denotes .
T5175 21678-21681 VBD denotes was
T5174 21668-21670 CD denotes 10
T4530 19916-19918 , denotes ,
T4529 19911-19916 NN denotes heart
T4528 19909-19911 , denotes ,
T4527 19904-19909 NN denotes liver
T4526 19901-19903 IN denotes in
T4525 19893-19900 NNS denotes changes
T4524 19887-19892 JJ denotes fatty
T4523 19884-19886 IN denotes of
T4522 19876-19883 NNS denotes degrees
T4521 19868-19875 VBG denotes varying
T4520 19860-19867 VBP denotes display
T4519 19856-19859 CC denotes and
T4518 19845-19855 JJ denotes intolerant
T4517 19840-19844 NN denotes cold
T4516 19828-19835 NNS denotes mutants
T4515 19822-19827 DT denotes these
T4514 19819-19821 IN denotes of
T4513 19836-19839 VBP denotes are
T4512 19815-19818 DT denotes All
T4511 19814-19929 sentence denotes All of these mutants are cold intolerant and display varying degrees of fatty changes in liver, heart, and kidney.
T4510 19813-19814 . denotes .
T4509 19812-19813 -RRB- denotes ]
T4508 19809-19810 , denotes ,
T4507 19807-19809 CD denotes 21
T4506 19806-19807 , denotes ,
T4505 19804-19806 CD denotes 16
T4504 19810-19812 CD denotes 22
T4503 19803-19804 -LRB- denotes [
T4502 19790-19795 NN denotes mouse
T4501 19788-19789 SYM denotes −
T4500 19787-19788 HYPH denotes /
T4499 19786-19787 SYM denotes −
T4498 19782-19786 NN denotes SCAD
T4497 19779-19781 CC denotes or
T4496 19777-19778 SYM denotes −
T4495 19776-19777 HYPH denotes /
T4494 19775-19776 SYM denotes −
T4493 19770-19775 NN denotes VLCAD
T4492 19796-19802 NNS denotes models
T4491 19766-19769 DT denotes the
T4490 19761-19765 IN denotes than
T4489 19750-19760 JJ denotes pronounced
T4488 19745-19749 RBR denotes more
T4487 19741-19744 CC denotes yet
T4486 19739-19741 , denotes ,
T4485 19733-19734 SYM denotes −
T4484 19732-19733 HYPH denotes /
T4483 19731-19732 SYM denotes −
T4482 19735-19739 NNS denotes mice
T4481 19727-19731 NN denotes LCAD
T4480 19724-19726 IN denotes in
T4479 19718-19723 VBN denotes found
T4478 19713-19717 DT denotes that
T4477 19708-19712 IN denotes than
T4476 19701-19707 JJ denotes severe
T4475 19696-19700 RBR denotes less
T4474 19686-19687 SYM denotes −
T4473 19685-19686 HYPH denotes /
T4472 19684-19685 SYM denotes −
T4471 19688-19692 NNS denotes mice
T4470 19680-19684 NN denotes MCAD
T4469 19677-19679 IN denotes of
T4468 19659-19666 JJ denotes overall
T4467 19667-19676 NN denotes phenotype
T4466 19655-19658 DT denotes the
T4465 19653-19655 , denotes ,
T4464 19641-19653 NNS denotes deficiencies
T4463 19627-19640 NN denotes dehydrogenase
T4462 19622-19623 HYPH denotes -
T4461 19623-19626 NN denotes CoA
T4460 19618-19622 NN denotes acyl
T4459 19614-19617 IN denotes for
T4458 19601-19606 NN denotes mouse
T4457 19595-19600 JJ denotes other
T4456 19607-19613 NNS denotes models
T4455 19591-19594 DT denotes the
T4454 19586-19590 IN denotes with
T4453 19574-19585 NNS denotes experiences
T4452 19570-19573 PRP$ denotes our
T4451 19565-19569 IN denotes with
T4450 19553-19564 NNS denotes comparisons
T4449 19693-19695 VBZ denotes is
T4448 19550-19552 IN denotes In
T4447 19549-19814 sentence denotes In comparisons with our experiences with the other mouse models for acyl-CoA dehydrogenase deficiencies, the overall phenotype of MCAD−/− mice is less severe than that found in LCAD−/− mice, yet more pronounced than the VLCAD−/− or SCAD−/− mouse models [16,21,22].
T4446 19548-19549 . denotes .
T4445 19547-19548 -RRB- denotes ]
T4444 19544-19545 , denotes ,
T4443 19543-19544 CD denotes 1
T4442 19545-19547 CD denotes 20
T4441 19542-19543 -LRB- denotes [
T4440 19535-19541 NNS denotes humans
T4439 19532-19534 IN denotes in
T4438 19521-19531 NN denotes deficiency
T4437 19516-19520 NN denotes MCAD
T4436 19513-19515 IN denotes in
T4435 19508-19512 VBN denotes seen
T4434 19483-19493 JJ denotes phenotypic
T4433 19465-19468 CC denotes and
T4432 19463-19464 HYPH denotes -
T4431 19469-19482 JJ denotes intrafamilial
T4430 19458-19463 AFX denotes inter
T4429 19446-19457 JJ denotes significant
T4428 19494-19507 NN denotes heterogeneity
T4427 19442-19445 DT denotes the
T4426 19437-19441 IN denotes with
T4425 19427-19436 JJ denotes analogous
T4424 19419-19423 NNS denotes mice
T4423 19413-19418 DT denotes these
T4422 19410-19412 IN denotes in
T4421 19424-19426 VBZ denotes is
T4420 19394-19401 JJ denotes cardiac
T4419 19390-19393 CC denotes and
T4418 19384-19389 NN denotes liver
T4417 19371-19383 JJ denotes inconsistent
T4416 19402-19409 NNS denotes lesions
T4415 19367-19370 DT denotes The
T4414 19366-19549 sentence denotes The inconsistent liver and cardiac lesions in these mice is analogous with the significant inter- and intrafamilial phenotypic heterogeneity seen in MCAD deficiency in humans [1,20].
T4413 19365-19366 . denotes .
T4412 19361-19365 NN denotes MCAD
T4411 19355-19360 NN denotes mouse
T4410 19352-19354 IN denotes of
T4409 19340-19351 NN denotes utilization
T4408 19330-19339 NN denotes substrate
T4407 19327-19329 IN denotes of
T4406 19313-19320 JJR denotes broader
T4405 19304-19312 JJ denotes apparent
T4404 19321-19326 NN denotes range
T4403 19300-19303 DT denotes the
T4402 19297-19299 IN denotes to
T4401 19291-19296 NN denotes mouse
T4400 19287-19290 DT denotes the
T4399 19284-19286 IN denotes in
T4398 19267-19274 JJ denotes cardiac
T4397 19275-19283 NNS denotes problems
T4396 19263-19266 DT denotes the
T4395 19256-19262 VB denotes relate
T4394 19253-19255 TO denotes to
T4393 19244-19252 JJ denotes tempting
T4392 19238-19240 PRP denotes it
T4391 19241-19243 VBZ denotes is
T4390 19233-19237 RB denotes Thus
T4389 19232-19366 sentence denotes Thus it is tempting to relate the cardiac problems in the mouse to the apparent broader range of substrate utilization of mouse MCAD.
T4388 19231-19232 . denotes .
T4387 19230-19231 -RRB- denotes ]
T4386 19229-19230 CD denotes 1
T4385 19228-19229 -LRB- denotes [
T4384 19213-19214 CD denotes 2
T4383 19212-19213 HYPH denotes -
T4382 19208-19211 CC denotes and
T4381 19205-19206 HYPH denotes -
T4380 19206-19207 CD denotes 1
T4379 19202-19205 NN denotes CPT
T4378 19215-19227 NNS denotes deficiencies
T4377 19195-19201 JJ denotes severe
T4376 19192-19194 IN denotes as
T4375 19187-19191 JJ denotes such
T4374 19176-19186 NN denotes metabolism
T4373 19172-19175 NN denotes fat
T4372 19166-19171 NN denotes chain
T4371 19161-19165 JJ denotes long
T4370 19158-19160 IN denotes of
T4369 19148-19157 NNS denotes disorders
T4368 19142-19147 JJ denotes other
T4367 19138-19141 CC denotes and
T4366 19136-19137 -RRB- denotes ]
T4365 19134-19136 CD denotes 19
T4364 19133-19134 -LRB- denotes [
T4363 19122-19132 NN denotes deficiency
T4362 19116-19121 NN denotes VLCAD
T4361 19113-19115 IN denotes in
T4360 19099-19103 VBN denotes been
T4359 19095-19098 VBZ denotes has
T4358 19080-19094 NN denotes cardiomyopathy
T4357 19078-19080 , denotes ,
T4356 19104-19112 VBN denotes observed
T4355 19065-19078 RB denotes Interestingly
T4354 19064-19232 sentence denotes Interestingly, cardiomyopathy has been observed in VLCAD deficiency [19] and other disorders of long chain fat metabolism such as severe CPT-1 and -2 deficiencies [1].
T4353 19063-19064 . denotes .
T4352 19062-19063 -RRB- denotes ]
T4351 19058-19060 , denotes ,
T4350 19056-19058 CD denotes 17
T4349 19060-19062 CD denotes 18
T4348 19055-19056 -LRB- denotes [
T4347 19046-19054 NNS denotes patients
T4346 19035-19036 HYPH denotes -
T4345 19036-19045 JJ denotes deficient
T4344 19031-19035 NN denotes MCAD
T4343 19028-19030 IN denotes in
T4342 19014-19018 VBN denotes been
T4341 19009-19013 VBP denotes have
T4957 20651-20655 NN denotes SalI
T4956 20656-20660 NN denotes site
T4955 20647-20650 DT denotes the
T4954 20642-20646 IN denotes into
T4953 20628-20631 VBD denotes was
T4952 20626-20627 -RRB- denotes ]
T4951 20624-20626 CD denotes 23
T4950 20623-20624 -LRB- denotes [
T4949 20632-20641 VBN denotes subcloned
T4948 20609-20613 NN denotes gene
T4947 20598-20608 NN denotes resistance
T4946 20589-20597 NN denotes neomycin
T4945 20614-20622 NN denotes cassette
T4944 20587-20588 DT denotes A
T4943 20586-20676 sentence denotes A neomycin resistance gene cassette [23] was subcloned into the SalI site of pGEM11Zf(+).
T4942 20585-20586 . denotes .
T4941 20569-20578 NN denotes targeting
T4940 20579-20585 NN denotes vector
T4939 20564-20568 NN denotes MCAD
T4938 20561-20563 IN denotes of
T4937 20548-20560 NN denotes Construction
T4340 18997-19008 NN denotes dysfunction
T4339 18993-18996 CC denotes and
T4338 18981-18992 NNS denotes arrhythmias
T4337 18973-18980 JJ denotes cardiac
T4336 18971-18973 , denotes ,
T4335 18964-18971 RB denotes however
T4334 18962-18964 , denotes ,
T4333 18949-18953 NN denotes MCAD
T4332 18954-18962 NNS denotes patients
T4331 18943-18948 JJ denotes human
T4330 18940-18942 IN denotes in
T4329 19019-19027 VBN denotes reported
T4328 18926-18930 VBN denotes been
T4327 18922-18925 RB denotes not
T4326 18918-18921 VBZ denotes has
T4325 18911-18912 SYM denotes −
T4324 18910-18911 HYPH denotes /
T4323 18909-18910 SYM denotes −
T4322 18913-18917 NNS denotes mice
T4321 18905-18909 NN denotes MCAD
T4320 18902-18904 IN denotes in
T4319 18893-18901 VBN denotes observed
T4318 18884-18892 NN denotes necrosis
T4317 18880-18883 CC denotes and
T4316 18859-18866 NN denotes myocyte
T4315 18867-18879 NN denotes degeneration
T4305 18792-18795 CC denotes and
T4304 18780-18791 JJ denotes interesting
T4303 18807-18814 NN denotes finding
T4302 18777-18779 DT denotes an
T4301 18770-18772 , denotes ,
T4300 18763-18770 RB denotes however
T4299 18761-18763 , denotes ,
T4298 18755-18756 SYM denotes −
T4297 18754-18755 HYPH denotes /
T4296 18753-18754 SYM denotes −
T4295 18757-18761 NNS denotes mice
T4294 18749-18753 NN denotes MCAD
T4293 18746-18748 IN denotes in
T4292 18772-18776 VBD denotes were
T4291 18730-18737 JJ denotes cardiac
T4290 18738-18745 NNS denotes lesions
T4289 18721-18729 JJ denotes Sporadic
T4288 18720-18815 sentence denotes Sporadic cardiac lesions in MCAD−/− mice, however, were an interesting and unexpected finding.
T4287 18719-18720 . denotes .
T4286 18713-18719 NN denotes stress
T4285 18705-18712 NN denotes fasting
T4284 18700-18704 IN denotes with
T4283 18686-18690 NN denotes MCAD
T4282 18691-18699 NNS denotes patients
T4281 18680-18685 JJ denotes human
T4280 18677-18679 IN denotes in
T4279 18672-18676 VBN denotes seen
T4278 18651-18663 JJ denotes pathological
T4277 18643-18650 JJ denotes primary
T4276 18664-18671 NN denotes finding
T4275 18639-18642 DT denotes the
T4274 18634-18638 IN denotes with
T4273 18623-18633 JJ denotes consistent
T4272 18613-18614 SYM denotes −
T4271 18612-18613 HYPH denotes /
T4270 18611-18612 SYM denotes −
T4269 18607-18611 NN denotes MCAD
T4268 18615-18619 NNS denotes mice
T4267 18600-18606 VBN denotes fasted
T4266 18597-18599 IN denotes in
T4265 18592-18596 VBN denotes seen
T4264 18620-18622 VBZ denotes is
T4263 18574-18581 JJ denotes hepatic
T4262 18559-18573 JJ denotes macrovesicular
T4261 18555-18558 CC denotes and
T4260 18540-18554 JJ denotes microvesicular
T4259 18582-18591 NN denotes steatosis
T4258 18536-18539 DT denotes The
T4257 18535-18720 sentence denotes The microvesicular and macrovesicular hepatic steatosis seen in fasted MCAD−/− mice is consistent with the primary pathological finding seen in human MCAD patients with fasting stress.
T4256 18534-18535 . denotes .
T4255 18530-18534 NN denotes mRNA
T4254 18524-18529 NN denotes Acadm
T4253 18521-18523 IN denotes of
T4252 18514-18520 NNS denotes levels
T4251 18509-18513 JJ denotes high
T4250 18499-18508 VBZ denotes expresses
T4249 18490-18498 RB denotes normally
T4248 18486-18489 CC denotes and
T4247 18472-18485 NN denotes thermogenesis
T4246 18468-18471 IN denotes for
T4245 18456-18467 JJ denotes responsible
T4244 18442-18455 RB denotes predominantly
T4243 18439-18441 VBZ denotes is
T4242 18435-18438 NN denotes fat
T4241 18429-18434 JJ denotes Brown
T4240 18428-18535 sentence denotes Brown fat is predominantly responsible for thermogenesis and normally expresses high levels of Acadm mRNA.
T4239 18427-18428 . denotes .
T4238 18416-18427 NN denotes temperature
T4237 18411-18415 NN denotes body
T4236 18402-18410 VB denotes maintain
T4235 18399-18401 TO denotes to
T4234 18392-18398 JJ denotes unable
T4233 18380-18381 SYM denotes −
T4232 18379-18380 HYPH denotes /
T4231 18378-18379 SYM denotes −
T4230 18382-18386 NNS denotes mice
T4229 18374-18378 NN denotes MCAD
T4228 18372-18374 , denotes ,
T4227 18365-18372 RB denotes however
T4226 18363-18365 , denotes ,
T4225 18353-18363 NNS denotes conditions
T4224 18343-18352 NN denotes challenge
T4223 18338-18342 NN denotes cold
T4222 18387-18391 VBD denotes were
T4221 18332-18337 IN denotes Under
T4220 18331-18428 sentence denotes Under cold challenge conditions, however, MCAD−/− mice were unable to maintain body temperature.
T4219 18330-18331 . denotes .
T4218 18323-18330 NN denotes fasting
T4217 18319-18322 CC denotes and
T4216 18312-18318 NN denotes stress
T4215 18307-18311 NN denotes cold
T4214 18302-18306 IN denotes with
T4213 18291-18301 VBN denotes challenged
T4212 18284-18290 IN denotes unless
T4211 18277-18278 SYM denotes −
T4210 18276-18277 HYPH denotes /
T4209 18275-18276 SYM denotes −
T4208 18271-18275 NN denotes MCAD
T4207 18279-18283 NNS denotes mice
T4206 18265-18270 JJ denotes adult
T4205 18262-18264 IN denotes in
T4204 18252-18255 VBD denotes was
T4203 18242-18251 NN denotes mortality
T4202 18239-18241 DT denotes no
T4201 18237-18239 , denotes ,
T4200 18229-18237 NN denotes contrast
T4199 18256-18261 VBN denotes noted
T4198 18226-18228 IN denotes In
T4197 18225-18331 sentence denotes In contrast, no mortality was noted in adult MCAD−/− mice unless challenged with cold stress and fasting.
T4196 18224-18225 . denotes .
T4195 18212-18215 RB denotes not
T4194 18209-18211 VBZ denotes is
T4193 18204-18208 NN denotes milk
T4192 18195-18203 JJ denotes maternal
T4191 18216-18224 VBN denotes ingested
T4190 18192-18194 IN denotes if
T4189 18187-18191 NN denotes time
T4188 18184-18186 IN denotes of
T4187 18171-18176 JJ denotes short
T4186 18177-18183 NN denotes period
T4185 18169-18170 DT denotes a
T4184 18166-18168 IN denotes in
T4183 18151-18165 NN denotes decompensation
T4182 18146-18150 IN denotes with
T4181 18138-18145 NN denotes fasting
T4180 18135-18137 IN denotes to
T4179 18123-18134 NN denotes sensitivity
T4178 18107-18110 VBP denotes are
T4177 18100-18101 SYM denotes −
T4176 18099-18100 HYPH denotes /
T4175 18098-18099 SYM denotes −
T4174 18094-18098 NN denotes MCAD
T4173 18102-18106 NNS denotes pups
T4172 18085-18093 JJ denotes neonatal
T4171 18111-18122 VBG denotes manifesting
T4170 18080-18084 IN denotes that
T4169 18073-18079 JJ denotes likely
T4168 18070-18072 VBZ denotes is
T4167 18067-18069 PRP denotes It
T4166 18066-18225 sentence denotes It is likely that neonatal MCAD−/− pups are manifesting sensitivity to fasting with decompensation in a short period of time if maternal milk is not ingested.
T4165 18065-18066 . denotes .
T4164 18064-18065 -RRB- denotes ]
T4163 18063-18064 CD denotes 1
T4162 18062-18063 -LRB- denotes [
T4161 18057-18061 NN denotes life
T4160 18054-18056 IN denotes in
T4159 18048-18050 CD denotes 24
T4158 18042-18047 JJ denotes first
T4157 18051-18053 NN denotes mo
T4156 18038-18041 DT denotes the
T4155 18031-18037 IN denotes during
T4154 18020-18030 RB denotes frequently
T4153 18015-18019 RBS denotes most
T4152 18013-18015 , denotes ,
T4151 18006-18013 NN denotes fasting
T4150 18003-18005 IN denotes by
T4149 17990-18002 VBN denotes precipitated
T4148 17973-17980 JJ denotes organic
T4147 17981-17989 NN denotes aciduria
T4146 17962-17972 JJ denotes nonketotic
T4145 17958-17961 CC denotes and
T4144 17956-17958 , denotes ,
T4143 17943-17956 NN denotes hypoketonemia
T4142 17941-17943 , denotes ,
T4141 17929-17941 NN denotes hypoglycemia
T4140 17924-17928 IN denotes with
T4139 17916-17923 VBP denotes present
T4138 17907-17915 NNS denotes patients
T4137 17901-17906 JJ denotes Human
T4136 17900-18066 sentence denotes Human patients present with hypoglycemia, hypoketonemia, and nonketotic organic aciduria precipitated by fasting, most frequently during the first 24 mo in life [1].
T4135 17899-17900 . denotes .
T4134 17896-17899 NN denotes age
T4133 17893-17895 IN denotes of
T4132 17890-17892 NN denotes wk
T4131 17888-17889 CD denotes 3
T4130 17885-17887 IN denotes at
T4129 17877-17884 NN denotes weaning
T4128 17874-17876 IN denotes to
T4127 17868-17873 JJ denotes prior
T4126 17855-17856 SYM denotes −
T4125 17854-17855 HYPH denotes /
T4124 17853-17854 SYM denotes −
T4123 17849-17853 NN denotes MCAD
T4122 17857-17861 NNS denotes pups
T4121 17845-17848 DT denotes the
T4120 17842-17844 IN denotes of
T4119 17862-17867 VBG denotes dying
T4118 17840-17841 NN denotes %
T4117 17838-17840 CD denotes 60
T4116 17824-17837 RB denotes approximately
T4651 20522-20523 . denotes .
T4650 20505-20513 JJ denotes relevant
T4649 20514-20522 NNS denotes diseases
T4648 20499-20504 JJ denotes other
T4647 20496-20498 IN denotes in
T4646 20490-20495 NNS denotes acids
T4645 20484-20489 JJ denotes fatty
T4644 20481-20483 IN denotes of
T4643 20476-20480 NN denotes role
T4642 20472-20475 DT denotes the
T4641 20454-20460 RBR denotes better
T4640 20461-20471 VB denotes understand
T4639 20451-20453 TO denotes to
T4638 20439-20445 JJ denotes robust
T4637 20446-20450 NN denotes tool
T4636 20437-20438 DT denotes a
T4635 20429-20436 VB denotes provide
T4634 20424-20428 MD denotes will
T4633 20420-20423 CC denotes and
T4632 20396-20397 HYPH denotes -
T4631 20397-20406 NN denotes oxidation
T4630 20395-20396 NN denotes β
T4629 20407-20419 NNS denotes deficiencies
T4628 20381-20394 JJ denotes mitochondrial
T4627 20378-20380 IN denotes of
T4626 20365-20377 NN denotes pathogenesis
T4625 20361-20364 DT denotes the
T4624 20356-20360 IN denotes into
T4623 20347-20355 NNS denotes insights
T4622 20343-20346 JJ denotes new
T4621 20336-20342 VBZ denotes offers
T4620 20319-20320 HYPH denotes -
T4619 20320-20329 JJ denotes deficient
T4618 20315-20319 JJ denotes MCAD
T4617 20330-20335 NN denotes mouse
T4616 20311-20314 DT denotes The
T4615 20310-20523 sentence denotes The MCAD-deficient mouse offers new insights into the pathogenesis of mitochondrial β-oxidation deficiencies and will provide a robust tool to better understand the role of fatty acids in other relevant diseases.
T4614 20309-20310 . denotes .
T4613 20308-20309 -RRB- denotes ]
T4612 20305-20306 , denotes ,
T4611 20303-20305 CD denotes 16
T4610 20306-20308 CD denotes 22
T4609 20302-20303 -LRB- denotes [
T4608 20288-20292 JJ denotes cold
T4607 20284-20287 RB denotes not
T4606 20280-20283 VBP denotes are
T4605 20272-20279 NNS denotes animals
T4604 20268-20271 DT denotes the
T4603 20293-20301 VBN denotes stressed
T4602 20265-20267 IN denotes if
T4601 20260-20264 JJ denotes mild
T4600 20249-20259 RB denotes relatively
T4599 20238-20239 SYM denotes −
T4598 20237-20238 HYPH denotes /
T4597 20236-20237 SYM denotes −
T4596 20232-20236 NN denotes SCAD
T4595 20228-20231 CC denotes and
T4594 20226-20227 SYM denotes −
T4593 20225-20226 HYPH denotes /
T4592 20224-20225 SYM denotes −
T4591 20219-20224 NN denotes VLCAD
T4590 20215-20218 DT denotes the
T4589 20240-20244 NNS denotes mice
T4588 20210-20214 CC denotes both
T4587 20207-20209 IN denotes of
T4586 20245-20248 VBP denotes are
T4585 20196-20206 NNS denotes phenotypes
T4584 20192-20195 DT denotes The
T4583 20191-20310 sentence denotes The phenotypes of both the VLCAD−/− and SCAD−/− mice are relatively mild if the animals are not cold stressed [16,22].
T4582 20190-20191 . denotes .
T4581 20179-20190 NN denotes intolerance
T4580 20171-20178 NN denotes fasting
T4579 20168-20170 IN denotes to
T4578 20156-20167 NN denotes sensitivity
T4577 20153-20155 IN denotes of
T4576 20138-20145 JJR denotes greater
T4575 20146-20152 NN denotes degree
T4574 20136-20137 DT denotes a
T4573 20125-20135 VBG denotes suggesting
T4572 20112-20117 NN denotes mouse
T4571 20106-20111 JJ denotes other
T4570 20118-20124 NNS denotes models
T4569 20100-20105 DT denotes these
T4568 20095-20099 IN denotes from
T4567 20083-20094 JJ denotes distinctive
T4566 20073-20074 SYM denotes −
T4565 20072-20073 HYPH denotes /
T4564 20071-20072 SYM denotes −
T4563 20075-20079 NNS denotes mice
T4562 20067-20071 NN denotes MCAD
T4561 20064-20066 IN denotes in
T4560 20080-20082 VBZ denotes is
T4559 20045-20053 JJ denotes neonatal
T4558 20033-20044 JJ denotes significant
T4557 20054-20063 NN denotes mortality
T4556 20029-20032 DT denotes The
T4555 20028-20191 sentence denotes The significant neonatal mortality in MCAD−/− mice is distinctive from these other mouse models suggesting a greater degree of sensitivity to fasting intolerance.
T4554 20027-20028 . denotes .
T4553 20026-20027 -RRB- denotes ]
T4552 20024-20026 CD denotes 21
T4551 20023-20024 -LRB- denotes [
T4550 20004-20009 JJ denotes other
T4549 20010-20022 NNS denotes deficiencies
T4548 20000-20003 DT denotes the
T4547 19995-19999 IN denotes than
T4546 19988-19994 NNS denotes losses
T4545 19976-19987 JJ denotes gestational
T4544 19972-19975 CC denotes and
T4543 19953-19964 JJ denotes spontaneous
T4542 19965-19971 NNS denotes deaths
T4541 19948-19952 JJR denotes more
T4540 19943-19947 VBP denotes show
T4539 19936-19937 SYM denotes −
T4538 19935-19936 HYPH denotes /
T4537 19934-19935 SYM denotes −
T4536 19938-19942 NNS denotes mice
T4535 19930-19934 NN denotes LCAD
T4534 19929-20028 sentence denotes LCAD−/− mice show more spontaneous deaths and gestational losses than the other deficiencies [21].
T4533 19928-19929 . denotes .
T4532 19922-19928 NN denotes kidney
T4531 19918-19921 CC denotes and
T4115 17819-17823 IN denotes with
T4114 17800-17808 JJ denotes neonatal
T4113 17809-17818 NN denotes mortality
T4112 17788-17799 JJ denotes significant
T4111 17778-17787 VBD denotes exhibited
T4110 17771-17772 SYM denotes −
T4109 17770-17771 HYPH denotes /
T4107 17773-17777 NNS denotes mice
T4106 17765-17769 NN denotes MCAD
T4105 17764-17900 sentence denotes MCAD−/− mice exhibited significant neonatal mortality with approximately 60% of the MCAD−/− pups dying prior to weaning at 3 wk of age.
T4104 17763-17764 . denotes .
T4103 17755-17763 NNS denotes patients
T4102 17744-17745 HYPH denotes -
T4101 17745-17754 JJ denotes deficient
T4100 17740-17744 NN denotes MCAD
T4099 17737-17739 IN denotes in
T4098 17727-17736 NN denotes mortality
T4097 17723-17726 CC denotes and
T4096 17714-17722 NNS denotes episodes
T4095 17705-17713 JJ denotes clinical
T4094 17702-17704 IN denotes of
T4093 17693-17701 NNS denotes patterns
T4092 17689-17692 DT denotes the
T4091 17686-17688 IN denotes to
T4090 17676-17685 JJ denotes analogous
T4058 17523-17525 IN denotes of
T4057 17514-17522 NN denotes activity
T4056 17510-17513 DT denotes the
T4055 17495-17499 MD denotes must
T4054 17480-17483 CD denotes two
T4053 17484-17494 NNS denotes substrates
T4052 17474-17479 DT denotes these
T4051 17469-17473 IN denotes with
T4050 17460-17468 VBN denotes measured
T4049 17437-17450 NN denotes dehydrogenase
T4048 17428-17436 JJ denotes residual
T4047 17451-17459 NN denotes activity
T4046 17424-17427 DT denotes the
T4045 17422-17424 , denotes ,
T4044 17416-17417 SYM denotes −
T4043 17415-17416 HYPH denotes /
T4042 17414-17415 SYM denotes −
T4041 17418-17422 NNS denotes mice
T4040 17410-17414 NN denotes MCAD
T4039 17407-17409 IN denotes in
T4038 17398-17406 VBN denotes detected
T4037 17385-17389 NN denotes MCAD
T4036 17390-17397 NN denotes antigen
T4035 17382-17384 DT denotes no
T4034 17374-17381 RB denotes clearly
T4033 17500-17509 VB denotes represent
T4032 17364-17369 EX denotes there
T4031 17370-17373 VBD denotes was
T4030 17356-17363 IN denotes Because
T4029 17355-17578 sentence denotes Because there was clearly no MCAD antigen detected in MCAD−/− mice, the residual dehydrogenase activity measured with these two substrates must represent the activity of other chain length–specific acyl-CoA dehydrogenases.
T4028 17354-17355 . denotes .
T4027 17353-17354 -RRB- denotes )
T4026 17352-17353 CD denotes 0
T4025 17351-17352 : denotes :
T4024 17348-17351 NN denotes C16
T4023 17347-17348 -LRB- denotes (
T4022 17342-17343 HYPH denotes -
T4021 17343-17346 NN denotes CoA
T4020 17333-17342 NN denotes palmitoyl
T4019 17328-17332 IN denotes with
T4018 17325-17327 RB denotes so
T4017 17320-17324 RBR denotes less
T4016 17316-17319 VBD denotes was
T4015 17311-17315 DT denotes This
T4014 17310-17355 sentence denotes This was less so with palmitoyl-CoA (C16:0).
T4013 17309-17310 . denotes .
T4012 17304-17305 HYPH denotes -
T4011 17305-17308 NN denotes CoA
T4010 17302-17304 NN denotes C8
T4009 17297-17301 IN denotes with
T4008 17292-17296 CD denotes zero
T4007 17287-17291 IN denotes near
T4006 17284-17286 IN denotes to
T4005 17273-17275 VBZ denotes is
T4004 17264-17272 NN denotes activity
T4003 17259-17263 NN denotes MCAD
T4002 17276-17283 VBN denotes reduced
T4001 17253-17258 WRB denotes where
T4000 17244-17252 NNS denotes patients
T3999 17238-17243 JJ denotes human
T3998 17235-17237 IN denotes in
T3997 17230-17234 NN denotes case
T3996 17226-17229 DT denotes the
T3995 17223-17225 VBZ denotes is
T3994 17220-17222 IN denotes as
T3993 17218-17220 , denotes ,
T3992 17214-17215 HYPH denotes -
T3991 17215-17218 NN denotes CoA
T3990 17212-17214 NN denotes C8
T3989 17198-17211 VB denotes dehydrogenate
T3988 17195-17197 TO denotes to
T3987 17179-17186 VBN denotes reduced
T3986 17165-17178 RB denotes significantly
T3985 17187-17194 NN denotes ability
T3984 17163-17164 DT denotes a
T3983 17159-17162 VBD denotes had
T3982 17152-17153 SYM denotes −
T3981 17151-17152 HYPH denotes /
T3980 17150-17151 SYM denotes −
T3979 17154-17158 NNS denotes mice
T3978 17146-17150 NN denotes MCAD
T3977 17145-17310 sentence denotes MCAD−/− mice had a significantly reduced ability to dehydrogenate C8-CoA, as is the case in human patients where MCAD activity is reduced to near zero with C8-CoA .
T3976 17144-17145 . denotes .
T3975 17129-17138 JJ denotes enzymatic
T3974 17139-17144 NN denotes level
T3973 17125-17128 DT denotes the
T3972 17122-17124 IN denotes at
T3971 17115-17116 SYM denotes −
T3970 17114-17115 HYPH denotes /
T3969 17113-17114 SYM denotes −
T3968 17109-17113 NN denotes MCAD
T3967 17117-17121 NNS denotes mice
T3966 17105-17108 DT denotes the
T3965 17092-17104 VB denotes characterize
T3964 17089-17091 TO denotes to
T3963 17074-17078 VBD denotes were
T3962 17062-17073 NNS denotes homogenates
T3961 17056-17061 NN denotes liver
T3960 17053-17055 IN denotes of
T3959 17079-17088 VBN denotes performed
T3958 17036-17045 NN denotes reduction
T3957 17046-17052 NNS denotes assays
T3956 17032-17035 NN denotes ETF
T3955 17031-17145 sentence denotes ETF reduction assays of liver homogenates were performed to characterize the MCAD−/− mice at the enzymatic level.
T3954 17030-17031 . denotes .
T3953 17029-17030 -RRB- denotes ]
T3952 17027-17029 CD denotes 16
T3951 17026-17027 -LRB- denotes [
T3950 17014-17015 HYPH denotes -
T3949 17010-17014 NN denotes acyl
T3948 17015-17025 NNS denotes substrates
T3947 17006-17009 NN denotes C14
T3946 16999-17005 IN denotes toward
T3945 16980-16989 JJ denotes enzymatic
T3944 16975-16979 RBS denotes most
T3943 16990-16998 NN denotes activity
T3942 16971-16974 DT denotes the
T3941 16966-16970 IN denotes with
T3940 16960-16965 NN denotes VLCAD
T3939 16954-16959 JJ denotes human
T3938 16951-16953 IN denotes to
T3937 16942-16950 VBN denotes compared
T3936 16939-16941 IN denotes as
T3935 16927-16928 HYPH denotes -
T3934 16923-16927 NN denotes acyl
T3933 16928-16938 NNS denotes substrates
T3932 16919-16922 NN denotes C16
T3931 16912-16918 IN denotes toward
T3930 16905-16911 JJ denotes active
T3929 16900-16904 RBS denotes most
T3928 16891-16896 NN denotes VLCAD
T3927 16885-16890 NN denotes mouse
T3926 16897-16899 VBZ denotes is
T3925 16879-16884 WRB denotes where
T3924 16877-16878 -RRB- denotes )
T3923 16872-16877 NN denotes VLCAD
T3922 16871-16872 -LRB- denotes (
T3921 16852-16853 HYPH denotes -
T3920 16853-16856 NN denotes CoA
T3919 16848-16852 NN denotes acyl
T3918 16857-16870 NN denotes dehydrogenase
T3917 16841-16842 HYPH denotes -
T3916 16842-16847 NN denotes chain
T3915 16837-16841 JJ denotes long
T3914 16832-16836 RB denotes very
T3913 16827-16831 IN denotes with
T3912 16822-16826 VBN denotes seen
T3911 16817-16821 DT denotes that
T3910 16814-16816 IN denotes to
T3909 16806-16813 JJ denotes similar
T3908 16803-16805 VBZ denotes is
T3907 16795-16802 NN denotes finding
T3906 16790-16794 DT denotes This
T3905 16789-17031 sentence denotes This finding is similar to that seen with very long-chain acyl-CoA dehydrogenase (VLCAD) where mouse VLCAD is most active toward C16 acyl-substrates as compared to human VLCAD with the most enzymatic activity toward C14 acyl-substrates [16].
T3904 16788-16789 . denotes .
T3903 16777-16781 NN denotes MCAD
T3902 16771-16776 JJ denotes human
T3901 16782-16788 NN denotes enzyme
T3900 16767-16770 DT denotes the
T3899 16762-16766 IN denotes than
T3898 16745-16750 NN denotes chain
T3897 16751-16761 NNS denotes substrates
T3896 16738-16744 JJR denotes longer
T3895 16731-16737 IN denotes toward
T3894 16724-16730 JJ denotes active
T3893 16719-16723 RBR denotes more
T3892 16716-16718 VBZ denotes is
T3891 16711-16715 NN denotes MCAD
T3890 16705-16710 NN denotes mouse
T3889 16703-16705 , denotes ,
T3888 16696-16703 RB denotes however
T3887 16694-16696 , denotes ,
T3886 16685-16694 VBD denotes indicated
T3885 16676-16684 NN denotes analysis
T3884 16662-16675 NN denotes Acylcarnitine
T3883 16661-16789 sentence denotes Acylcarnitine analysis indicated, however, mouse MCAD is more active toward longer chain substrates than the human MCAD enzyme.
T3882 16660-16661 . denotes .
T3881 16654-16655 SYM denotes −
T3880 16653-16654 HYPH denotes /
T3879 16652-16653 SYM denotes −
T3878 16656-16660 NNS denotes mice
T3877 16648-16652 NN denotes MCAD
T3876 16645-16647 IN denotes in
T3875 16636-16639 VBD denotes was
T3874 16640-16644 VBN denotes seen
T3873 16633-16635 IN denotes as
T3872 16631-16633 , denotes ,
T3871 16611-16626 NN denotes hexanoylglycine
T3870 16603-16610 JJ denotes urinary
T3869 16588-16602 JJ denotes characteristic
T4314 18848-18858 JJ denotes multifocal
T4313 18843-18847 IN denotes with
T4312 18931-18939 VBN denotes reported
T4311 18820-18827 JJ denotes diffuse
T4310 18828-18842 NN denotes cardiomyopathy
T4309 18816-18819 DT denotes The
T4308 18815-19064 sentence denotes The diffuse cardiomyopathy with multifocal myocyte degeneration and necrosis observed in MCAD−/− mice has not been reported in human MCAD patients, however, cardiac arrhythmias and dysfunction have been reported in MCAD-deficient patients [17, 18].
T4307 18814-18815 . denotes .
T4306 18796-18806 JJ denotes unexpected
T3868 16627-16631 NN denotes peak
T3867 16586-16587 DT denotes a
T3866 16572-16577 RB denotes often
T3865 16570-16571 -RRB- denotes ]
T3864 16568-16570 CD denotes 15
T3863 16567-16568 -LRB- denotes [
T3862 16578-16585 VBP denotes display
T3861 16558-16566 NNS denotes patients
T3860 16547-16548 HYPH denotes -
T3859 16548-16557 JJ denotes deficient
T3858 16543-16547 NN denotes MCAD
T3857 16542-16661 sentence denotes MCAD-deficient patients [15] often display a characteristic urinary hexanoylglycine peak, as was seen in MCAD−/− mice.
T3856 16541-16542 . denotes .
T3855 16523-16529 NN denotes enzyme
T3854 16515-16516 HYPH denotes –
T3853 16516-16522 NN denotes length
T3852 16510-16515 NN denotes chain
T3851 16503-16509 JJ denotes medium
T3850 16530-16541 NN denotes specificity
T3849 16499-16502 DT denotes the
T3848 16494-16498 IN denotes with
T3847 16483-16493 VBN denotes associated
T3846 16465-16470 NN denotes urine
T3845 16461-16464 CC denotes and
T3844 16454-16460 NN denotes plasma
T3843 16471-16482 NNS denotes metabolites
T4089 17673-17675 VB denotes be
T4088 17670-17672 TO denotes to
T4087 17662-17669 VBZ denotes appears
T4086 17658-17661 CC denotes and
T4085 17637-17645 JJ denotes striking
T4084 17646-17657 NN denotes observation
T4083 17635-17636 DT denotes a
T4082 17624-17625 SYM denotes −
T4081 17623-17624 HYPH denotes /
T4080 17622-17623 SYM denotes −
T4079 17626-17630 NNS denotes mice
T4078 17618-17622 NN denotes MCAD
T4077 17615-17617 IN denotes in
T4076 17605-17614 NN denotes mortality
T4075 17596-17604 JJ denotes neonatal
T4074 17593-17595 IN denotes of
T4073 17631-17634 VBD denotes was
T4072 17581-17585 JJ denotes high
T4071 17586-17592 NN denotes degree
T4070 17579-17580 DT denotes A
T4069 17578-17764 sentence denotes A high degree of neonatal mortality in MCAD−/− mice was a striking observation and appears to be analogous to the patterns of clinical episodes and mortality in MCAD-deficient patients.
T4068 17577-17578 . denotes .
T4067 17558-17559 HYPH denotes -
T4066 17559-17562 NN denotes CoA
T4065 17554-17558 NN denotes acyl
T4064 17544-17545 HYPH denotes –
T4063 17545-17553 JJ denotes specific
T4062 17538-17544 NN denotes length
T4061 17532-17537 NN denotes chain
T4060 17563-17577 NNS denotes dehydrogenases
T4059 17526-17531 JJ denotes other
T3587 16228-16229 . denotes .
T3586 16214-16221 NN denotes adipose
T3585 16222-16228 NN denotes tissue
T3584 16205-16213 JJ denotes adjacent
T3583 16198-16204 IN denotes within
T3582 16183-16187 VBD denotes were
T3581 16188-16197 VBN denotes scattered
T3506 15790-15794 VBD denotes were
T3505 15762-15768 NNS denotes Nuclei
T3504 15761-15849 sentence denotes Nuclei of affected myocytes were large, pale, and vesicular and had prominent nucleoli.
T3503 15760-15761 . denotes .
T3502 15739-15751 JJ denotes eosinophilic
T3501 15752-15760 NN denotes material
T3500 15730-15738 JJ denotes granular
T3499 15723-15729 RB denotes finely
T3498 15718-15722 IN denotes with
T3497 15707-15717 NNS denotes myofibrils
T3496 15704-15706 IN denotes of
T3495 15690-15692 , denotes ,
T3494 15680-15690 NN denotes sarcoplasm
T3493 15676-15679 DT denotes the
T3492 15673-15675 IN denotes of
T3491 15664-15672 NNS denotes portions
T3490 15692-15703 NN denotes replacement
T3489 15661-15663 IN denotes in
T3488 15659-15661 , denotes ,
T3487 15656-15659 CC denotes and
T3486 15654-15656 , denotes ,
T3485 15646-15654 NN denotes staining
T3484 15641-15645 JJ denotes pale
T3483 15639-15641 , denotes ,
T3482 15631-15639 VBG denotes swelling
T3481 15613-15621 NNS denotes myocytes
T3480 15600-15612 VBG denotes degenerating
T3479 15597-15599 IN denotes in
T3478 15622-15630 VBD denotes included
T3477 15589-15596 NNS denotes Changes
T3476 15588-15761 sentence denotes Changes in degenerating myocytes included swelling, pale staining, and, in portions of the sarcoplasm, replacement of myofibrils with finely granular eosinophilic material.
T3475 15587-15588 . denotes .
T3474 15586-15587 -RRB- denotes )
T3473 15584-15586 CD denotes 5D
T3472 15580-15583 CC denotes and
T3471 15570-15576 NN denotes Figure
T3470 15577-15579 CD denotes 5C
T3469 15569-15570 -LRB- denotes (
T3468 15560-15568 NN denotes necrosis
T3467 15556-15559 CC denotes and
T3466 15535-15542 NN denotes myocyte
T3465 15524-15534 JJ denotes multifocal
T3464 15517-15523 JJ denotes active
T3463 15543-15555 NN denotes degeneration
T3462 15509-15516 JJ denotes chronic
T3461 15504-15508 IN denotes with
T3460 15489-15503 NN denotes cardiomyopathy
T3459 15481-15488 JJ denotes diffuse
T3458 15469-15470 SYM denotes −
T3457 15468-15469 HYPH denotes /
T3456 15467-15468 SYM denotes −
T3455 15463-15467 NN denotes MCAD
T3454 15471-15476 NN denotes mouse
T3453 15460-15462 DT denotes an
T3452 15458-15460 , denotes ,
T3451 15451-15458 NN denotes example
T3450 15447-15450 CD denotes one
T3449 15477-15480 VBD denotes had
T3448 15444-15446 IN denotes In
T3447 14972-15588 sentence denotes Figure 5 Histopathology of MCAD+/+ and MCAD−/− Mice (A) MCAD+/+ mice had no evidence of hepatic steatosis following a 24-h fast. Liver section with Oil-Red O stain. (B) Hepatosteatosis in MCAD−/− mouse following a 24-h fast. Oil-Red O stained liver sections revealed severe and diffuse microvesicular and macrovesicular hepatic steatosis in MCAD−/− mice. (C and D) Diffuse cardiomyopathy with chronic active multifocal myocyte degeneration and necrosis in MCAD−/− mice. In one example, an MCAD−/− mouse had diffuse cardiomyopathy with chronic active multifocal myocyte degeneration and necrosis (Figure 5C and 5D).
T3446 14971-14972 . denotes .
T3445 14965-14966 SYM denotes −
T3444 14964-14965 HYPH denotes /
T3443 14963-14964 SYM denotes −
T3442 14959-14963 NN denotes MCAD
T3441 14967-14971 NNS denotes mice
T3440 14950-14958 JJ denotes multiple
T3439 14947-14949 IN denotes in
T3438 14931-14938 JJ denotes cardiac
T3437 14939-14946 NNS denotes lesions
T3436 14922-14930 JJ denotes sporadic
T3435 14913-14915 PRP denotes we
T3434 14911-14913 , denotes ,
T3433 14903-14911 NN denotes addition
T3432 14916-14921 VBD denotes found
T3431 14900-14902 IN denotes In
T3430 14899-14972 sentence denotes In addition, we found sporadic cardiac lesions in multiple MCAD−/− mice.
T3429 14898-14899 . denotes .
T3428 14889-14898 NN denotes steatosis
T3427 14881-14888 JJ denotes hepatic
T3426 14866-14868 , denotes ,
T3425 14857-14866 RB denotes therefore
T3424 14855-14857 , denotes ,
T3423 14868-14880 VBD denotes demonstrated
T3422 14849-14850 SYM denotes −
T3421 14848-14849 HYPH denotes /
T3420 14847-14848 SYM denotes −
T3419 14843-14847 NN denotes MCAD
T3418 14837-14842 JJR denotes older
T3417 14833-14836 DT denotes the
T3416 14851-14855 NNS denotes mice
T3415 14828-14832 RB denotes Only
T3414 14827-14899 sentence denotes Only the older MCAD−/− mice, therefore, demonstrated hepatic steatosis.
T3413 14826-14827 . denotes .
T3412 14817-14826 VBN denotes evaluated
T3411 14810-14816 NNS denotes organs
T3410 14806-14809 DT denotes all
T3409 14803-14805 IN denotes in
T3408 14789-14802 NNS denotes abnormalities
T3407 14786-14788 DT denotes no
T3406 14783-14785 IN denotes to
T3405 14775-14782 JJ denotes minimal
T3404 14764-14769 EX denotes there
T3403 14762-14764 , denotes ,
T3402 14760-14761 HYPH denotes -
T3401 14761-14762 NN denotes h
T3400 14758-14760 CD denotes 24
T3399 14754-14757 IN denotes for
T3398 14747-14753 VBN denotes fasted
T3397 14740-14741 SYM denotes −
T3396 14739-14740 HYPH denotes /
T3395 14738-14739 SYM denotes −
T3394 14734-14738 NN denotes MCAD
T3393 14730-14733 CC denotes and
T3392 14728-14729 SYM denotes +
T3391 14727-14728 HYPH denotes /
T3390 14726-14727 SYM denotes +
T3389 14722-14726 NN denotes MCAD
T3388 14742-14746 NNS denotes mice
T3387 14717-14718 HYPH denotes -
T3386 14718-14721 JJ denotes old
T3385 14714-14715 HYPH denotes -
T3384 14715-14717 NN denotes wk
T3383 14713-14714 CD denotes 4
T3382 14710-14712 IN denotes of
T3381 14704-14709 NN denotes group
T3380 14696-14703 DT denotes another
T3379 14770-14774 VBD denotes were
T3378 14693-14695 IN denotes In
T3377 14692-14827 sentence denotes In another group of 4-wk-old MCAD+/+ and MCAD−/− mice fasted for 24-h, there were minimal to no abnormalities in all organs evaluated.
T3376 14691-14692 . denotes .
T3375 14690-14691 -RRB- denotes )
T3374 14688-14690 CD denotes 5B
T3373 14684-14687 CC denotes and
T3372 14674-14680 NN denotes Figure
T3371 14681-14683 CD denotes 5A
T3370 14673-14674 -LRB- denotes (
T3369 14654-14664 JJ denotes histologic
T3368 14665-14672 NNS denotes changes
T3367 14651-14653 DT denotes no
T3366 14640-14641 SYM denotes +
T3365 14639-14640 HYPH denotes /
T3364 14638-14639 SYM denotes +
T3363 14642-14646 NNS denotes mice
T3362 14634-14638 NN denotes MCAD
T3361 14647-14650 VBD denotes had
T3360 14626-14633 IN denotes whereas
T3359 14619-14620 SYM denotes −
T3358 14618-14619 HYPH denotes /
T3357 14617-14618 SYM denotes −
T3356 14613-14617 NN denotes MCAD
T3355 14621-14625 NNS denotes mice
T3354 14608-14609 HYPH denotes -
T3353 14609-14612 JJ denotes old
T3352 14605-14606 HYPH denotes -
T3351 14606-14608 NN denotes wk
T3350 14603-14604 SYM denotes –
T3349 14604-14605 CD denotes 8
T3348 14602-14603 CD denotes 6
T3347 14599-14601 IN denotes in
T3346 14581-14588 JJ denotes hepatic
T3345 14566-14580 JJ denotes macrovesicular
T3344 14562-14565 CC denotes and
T3343 14547-14561 JJ denotes microvesicular
T3342 14589-14598 NN denotes steatosis
T3341 14539-14546 JJ denotes diffuse
T3340 14518-14519 HYPH denotes -
T3339 14519-14520 NN denotes h
T3338 14516-14518 CD denotes 24
T3337 14521-14525 NN denotes fast
T3336 14514-14515 DT denotes a
T3335 14508-14513 IN denotes after
T3334 14495-14502 NN denotes control
T3333 14493-14494 SYM denotes +
T3332 14492-14493 HYPH denotes /
T3331 14491-14492 SYM denotes +
T3330 14487-14491 NN denotes MCAD
T3329 14483-14486 CC denotes and
T3328 14503-14507 NNS denotes mice
T3327 14476-14482 NN denotes mutant
T3326 14473-14475 IN denotes of
T3325 14467-14472 NN denotes group
T3324 14463-14466 CD denotes one
T3323 14460-14462 IN denotes of
T3322 14526-14538 VBD denotes demonstrated
T3321 14432-14447 JJ denotes histopathologic
T3320 14448-14459 NN denotes examination
T3319 14423-14431 JJ denotes Complete
T3318 14422-14692 sentence denotes Complete histopathologic examination of one group of mutant and MCAD+/+ control mice after a 24-h fast demonstrated diffuse microvesicular and macrovesicular hepatic steatosis in 6–8-wk-old MCAD−/− mice whereas MCAD+/+ mice had no histologic changes (Figure 5A and 5B).
T3317 14408-14422 NN denotes Histopathology
T3842 16445-16453 JJ denotes abnormal
T3841 16440-16444 VBP denotes have
T3840 16431-16439 NNS denotes patients
T3839 16420-16421 HYPH denotes -
T3838 16421-16430 JJ denotes deficient
T3837 16416-16420 NN denotes MCAD
T3836 16415-16542 sentence denotes MCAD-deficient patients have abnormal plasma and urine metabolites associated with the medium chain–length enzyme specificity.
T3835 16414-16415 . denotes .
T3834 16406-16414 NNS denotes patients
T3833 16400-16405 JJ denotes human
T3832 16397-16399 IN denotes in
T3831 16391-16396 VBN denotes found
T3830 16370-16380 JJ denotes pathologic
T3829 16366-16369 CC denotes and
T3828 16364-16366 , denotes ,
T3827 16353-16364 JJ denotes biochemical
T3826 16351-16353 , denotes ,
T3825 16343-16351 JJ denotes clinical
T3824 16381-16390 NN denotes phenotype
T3823 16339-16342 DT denotes the
T3822 16333-16338 VBP denotes mimic
T3821 16328-16332 WDT denotes that
T3820 16319-16327 NNS denotes features
T3819 16314-16318 IN denotes with
T3818 16303-16313 NN denotes deficiency
T3817 16298-16302 NN denotes MCAD
T3816 16294-16297 IN denotes for
T3815 16282-16287 NN denotes mouse
T3814 16288-16293 NN denotes model
T3813 16280-16281 DT denotes a
T3812 16265-16270 NN denotes Acadm
T3811 16271-16279 VBD denotes produced
T3810 16255-16264 VBG denotes targeting
T3809 16242-16254 RB denotes Successfully
T3078 13274-13291 NN denotes decenoylcarnitine
T3077 13268-13273 NN denotes serum
T3076 13265-13267 IN denotes of
T3075 13292-13299 JJ denotes evident
T3074 13249-13250 HYPH denotes -
T3073 13250-13254 JJ denotes fold
T3072 13245-13247 IN denotes to
T3071 13243-13244 HYPH denotes -
T3070 13248-13249 CD denotes 6
T3069 13242-13243 CD denotes 5
T3068 13255-13264 NN denotes elevation
T3067 13240-13241 DT denotes a
T3066 13236-13239 VBD denotes had
T3065 13229-13230 SYM denotes −
T3064 13228-13229 HYPH denotes /
T3063 13227-13228 SYM denotes −
T3062 13231-13235 NNS denotes mice
T3061 13223-13227 NN denotes MCAD
T3060 13219-13222 CC denotes but
T3059 13217-13219 , denotes ,
T3058 13211-13212 SYM denotes −
T3057 13210-13211 HYPH denotes /
T3056 13209-13210 SYM denotes −
T3055 13205-13209 NN denotes MCAD
T3054 13201-13204 CC denotes and
T3053 13199-13200 SYM denotes +
T3052 13198-13199 HYPH denotes /
T3051 13197-13198 SYM denotes +
T3050 13213-13217 NNS denotes mice
T3049 13193-13197 NN denotes MCAD
T3048 13185-13192 IN denotes between
T3047 13160-13169 NN denotes carnitine
T3046 13154-13159 NN denotes serum
T3045 13170-13184 NNS denotes concentrations
T3044 13148-13153 JJ denotes total
T3043 13145-13147 IN denotes in
T3042 13121-13132 JJ denotes significant
T3041 13133-13144 NNS denotes differences
T3040 13118-13120 DT denotes no
T3039 13102-13103 SYM denotes −
T3038 13101-13102 HYPH denotes /
T3037 13100-13101 SYM denotes −
T3036 13096-13100 NN denotes MCAD
T3035 13092-13095 CC denotes and
T3034 13090-13091 SYM denotes +
T3033 13089-13090 HYPH denotes /
T3032 13088-13089 SYM denotes +
T3031 13104-13108 NNS denotes mice
T3030 13084-13088 NN denotes MCAD
T3029 13081-13083 IN denotes of
T3028 13109-13117 VBD denotes revealed
T3027 13070-13080 NN denotes Comparison
T3026 13069-13341 sentence denotes Comparison of MCAD+/+ and MCAD−/− mice revealed no significant differences in total serum carnitine concentrations between MCAD+/+ and MCAD−/− mice, but MCAD−/− mice had a 5- to 6-fold elevation of serum decenoylcarnitine evident in the acylcarnitine profile (Figure 4A).
T3025 13068-13069 . denotes .
T3024 13067-13068 -RRB- denotes )
T3023 13058-13061 RB denotes not
T3022 13053-13057 NNS denotes data
T3021 13062-13067 VBN denotes shown
T3020 13052-13053 -LRB- denotes (
T3019 13042-13051 NNS denotes genotypes
T3018 13037-13041 NN denotes MCAD
T3017 13029-13036 IN denotes between
T3016 13005-13016 JJ denotes significant
T3015 13017-13028 NNS denotes differences
T3014 13002-13004 DT denotes no
T3013 12996-13001 VBD denotes found
T3012 12992-12995 CC denotes and
T3011 12965-12976 JJ denotes acetoacetic
T3010 12961-12964 CC denotes and
T3009 12977-12991 NNS denotes concentrations
T3008 12945-12946 HYPH denotes -
T3007 12946-12960 JJ denotes hydroxybutyric
T3006 12944-12945 NN denotes β
T3005 12929-12933 RB denotes also
T3004 12934-12943 VBD denotes evaluated
T3003 12926-12928 PRP denotes We
T3002 12925-13069 sentence denotes We also evaluated β-hydroxybutyric and acetoacetic concentrations and found no significant differences between MCAD genotypes (data not shown).
T3001 12924-12925 . denotes .
T3000 12914-12924 NN denotes deficiency
T2999 12909-12913 NN denotes MCAD
T2998 12906-12908 IN denotes to
T2997 12897-12905 JJ denotes specific
T2996 12893-12896 RB denotes not
T2995 12890-12892 VBZ denotes is
T2994 12885-12889 NN denotes acid
T2993 12878-12884 JJ denotes Adipic
T2992 12877-12925 sentence denotes Adipic acid is not specific to MCAD deficiency.
T2991 12876-12877 . denotes .
T2990 12875-12876 -RRB- denotes )
T2989 12874-12875 CD denotes 1
T2988 12868-12873 NN denotes Table
T2987 12867-12868 -LRB- denotes (
T2986 12853-12860 JJ denotes organic
T2985 12848-12852 JJ denotes same
T2984 12861-12866 NNS denotes acids
T2983 12844-12847 DT denotes the
T2982 12841-12843 IN denotes of
T2981 12822-12832 JJ denotes detectable
T2980 12833-12840 NNS denotes amounts
T2979 12819-12821 DT denotes no
T2978 12816-12818 IN denotes to
T2977 12810-12815 NN denotes trace
T2976 12803-12809 VBD denotes showed
T2975 12797-12802 WDT denotes which
T2974 12795-12797 , denotes ,
T2973 12785-12786 SYM denotes +
T2972 12784-12785 HYPH denotes /
T2971 12783-12784 SYM denotes +
T2970 12787-12795 NNS denotes controls
T2969 12779-12783 NN denotes MCAD
T2968 12776-12778 IN denotes to
T2967 12767-12775 VBN denotes compared
T2966 12764-12766 IN denotes as
T2965 12748-12763 NN denotes hexanoylglycine
T2964 12744-12747 CC denotes and
T2963 12730-12737 JJ denotes sebacic
T2962 12726-12729 CC denotes and
T2961 12724-12726 , denotes ,
T2960 12717-12724 JJ denotes suberic
T2959 12715-12717 , denotes ,
T2958 12738-12743 NNS denotes acids
T2957 12709-12715 JJ denotes adipic
T2956 12706-12708 IN denotes of
T2955 12691-12705 NNS denotes concentrations
T2954 12682-12690 VBN denotes elevated
T2953 12668-12681 RB denotes significantly
T2952 12651-12652 SYM denotes −
T2951 12650-12651 HYPH denotes /
T2950 12649-12650 SYM denotes −
T2949 12653-12657 NNS denotes mice
T2948 12645-12649 NN denotes MCAD
T2947 12643-12645 , denotes ,
T2946 12642-12643 NN denotes h
T2945 12639-12641 CD denotes 18
T2944 12635-12638 IN denotes for
T2943 12628-12634 VBN denotes fasted
T2942 12623-12627 WRB denotes when
T2941 12621-12623 , denotes ,
T2940 12658-12667 VBD denotes developed
T2939 12609-12621 RB denotes Specifically
T2938 12608-12877 sentence denotes Specifically, when fasted for 18 h, MCAD−/− mice developed significantly elevated concentrations of adipic, suberic, and sebacic acids and hexanoylglycine as compared to MCAD+/+ controls, which showed trace to no detectable amounts of the same organic acids (Table 1).
T2937 12607-12608 . denotes .
T2936 12593-12598 JJ denotes human
T2935 12599-12607 NNS denotes patients
T2934 12582-12583 HYPH denotes -
T2933 12583-12592 JJ denotes deficient
T2932 12578-12582 NN denotes MCAD
T2931 12575-12577 IN denotes to
T2930 12567-12574 JJ denotes similar
T2929 12554-12558 NN denotes acid
T2928 12546-12553 JJ denotes organic
T2927 12559-12566 NN denotes profile
T2926 12543-12545 DT denotes an
T2925 12526-12527 SYM denotes −
T2924 12525-12526 HYPH denotes /
T2923 12524-12525 SYM denotes −
T2922 12528-12532 NNS denotes mice
T2921 12520-12524 NN denotes MCAD
T2920 12533-12542 VBD denotes developed
T2919 12515-12519 IN denotes that
T2918 12506-12514 VBD denotes revealed
T2917 12497-12505 NN denotes analysis
T2916 12484-12491 JJ denotes organic
T2915 12492-12496 NN denotes acid
T2914 12478-12483 NN denotes Urine
T2913 12477-12608 sentence denotes Urine organic acid analysis revealed that MCAD−/− mice developed an organic acid profile similar to MCAD-deficient human patients.
T2912 12469-12477 NN denotes Analysis
T2911 12455-12468 NN denotes Acylcarnitine
T2910 12451-12454 CC denotes and
T2909 12446-12450 NN denotes Acid
T2908 12438-12445 JJ denotes Organic
T2718 12107-12111 NN denotes MCAD
T3580 16174-16182 NNS denotes deposits
T3579 16166-16173 JJ denotes Similar
T3578 16165-16229 sentence denotes Similar deposits were scattered within adjacent adipose tissue.
T3577 16164-16165 . denotes .
T3576 16153-16154 HYPH denotes /
T3575 16154-16164 NN denotes lipofuscin
T3574 16147-16153 NN denotes ceroid
T3573 16144-16146 VB denotes be
T3572 16141-16143 TO denotes to
T3571 16129-16140 VBN denotes interpreted
T3570 16114-16115 HYPH denotes -
T3569 16115-16120 JJ denotes brown
T3568 16108-16114 JJ denotes yellow
T3567 16096-16107 JJ denotes translucent
T3566 16121-16128 NN denotes pigment
T3565 16087-16095 JJ denotes globular
T3564 16084-16086 IN denotes of
T3563 16072-16083 NNS denotes collections
T3562 16061-16071 JJ denotes multifocal
T3561 16058-16060 IN denotes by
T3560 16046-16057 VBN denotes accompanied
T3559 16044-16046 , denotes ,
T3558 16030-16037 JJ denotes elastic
T3557 16038-16044 NN denotes tissue
T3556 16026-16029 DT denotes the
T3555 16023-16025 IN denotes of
T3554 16010-16022 NN denotes degeneration
T3553 15999-16009 JJ denotes multifocal
T3552 15989-15994 EX denotes there
T3551 15983-15988 NN denotes heart
T3550 15979-15982 DT denotes the
T3549 15976-15978 IN denotes of
T3548 15971-15975 NN denotes base
T3547 15967-15970 DT denotes the
T3546 15964-15966 IN denotes at
T3545 15958-15963 NN denotes aorta
T3544 15954-15957 DT denotes the
T3543 15951-15953 IN denotes of
T3542 15946-15950 NN denotes wall
T3541 15942-15945 DT denotes the
T3540 15995-15998 VBD denotes was
T3539 15939-15941 IN denotes In
T3538 15938-16165 sentence denotes In the wall of the aorta at the base of the heart there was multifocal degeneration of the elastic tissue, accompanied by multifocal collections of globular translucent yellow-brown pigment interpreted to be ceroid/lipofuscin.
T3537 15937-15938 . denotes .
T3536 15929-15937 NN denotes fibrosis
T3535 15926-15928 IN denotes by
T3534 15914-15925 VBN denotes accompanied
T3533 15905-15913 NNS denotes myocytes
T3532 15902-15904 IN denotes of
T3531 15897-15901 NN denotes loss
T3530 15887-15892 EX denotes there
T3529 15885-15887 , denotes ,
T3528 15871-15879 VBN denotes affected
T3527 15862-15870 RB denotes severely
T3526 15857-15861 RBS denotes most
T3525 15880-15885 NNS denotes areas
T3524 15853-15856 DT denotes the
T3523 15893-15896 VBD denotes was
T3522 15850-15852 IN denotes In
T3521 15849-15938 sentence denotes In the most severely affected areas, there was loss of myocytes accompanied by fibrosis.
T3520 15848-15849 . denotes .
T3519 15840-15848 NNS denotes nucleoli
T3518 15830-15839 JJ denotes prominent
T3517 15826-15829 VBD denotes had
T3516 15822-15825 CC denotes and
T3515 15812-15821 JJ denotes vesicular
T3514 15808-15811 CC denotes and
T3513 15806-15808 , denotes ,
T3512 15802-15806 JJ denotes pale
T3511 15800-15802 , denotes ,
T3510 15795-15800 JJ denotes large
T3509 15781-15789 NNS denotes myocytes
T3508 15772-15780 VBN denotes affected
T3507 15769-15771 IN denotes of
T2783 12340-12344 NN denotes MCAD
T2782 12335-12339 CD denotes four
T2781 12348-12352 NNS denotes mice
T2780 12331-12334 DT denotes all
T2779 12329-12331 , denotes ,
T2778 12321-12329 NN denotes contrast
T2777 12353-12361 VBD denotes survived
T2776 12318-12320 IN denotes In
T2775 12317-12436 sentence denotes In contrast, all four MCAD+/+ mice survived the 3-h cold stress, ending with an average rectal temperature of 33.6 °C.
T2774 12316-12317 . denotes .
T2773 12314-12316 NN denotes °C
T2772 12309-12313 CD denotes 22.7
T2771 12293-12294 SYM denotes −
T2770 12292-12293 HYPH denotes /
T2769 12291-12292 SYM denotes −
T2768 12287-12291 NN denotes MCAD
T2767 12277-12286 VBG denotes surviving
T2766 12273-12276 CD denotes two
T2765 12295-12299 NNS denotes mice
T2764 12269-12272 DT denotes the
T2763 12267-12269 , denotes ,
T2762 12260-12261 HYPH denotes -
T2761 12261-12262 NN denotes h
T2760 12257-12260 CD denotes 1.5
T2759 12263-12267 NN denotes mark
T2758 12253-12256 DT denotes the
T2757 12250-12252 IN denotes of
T2756 12246-12249 NN denotes end
T2755 12242-12245 DT denotes the
T2754 12300-12308 VBD denotes averaged
T2753 12239-12241 IN denotes By
T2752 12238-12317 sentence denotes By the end of the 1.5-h mark, the two surviving MCAD−/− mice averaged 22.7 °C.
T2751 12237-12238 . denotes .
T2750 12231-12232 SYM denotes −
T2749 12230-12231 HYPH denotes /
T2748 12229-12230 SYM denotes −
T2747 12225-12229 NN denotes MCAD
T2746 12233-12237 NNS denotes mice
T2745 12216-12219 DT denotes the
T2744 12213-12215 IN denotes of
T2743 12220-12224 CD denotes five
T2742 12207-12212 CD denotes three
T2741 12204-12206 IN denotes in
T2740 12201-12203 NN denotes °C
T2739 12195-12196 SYM denotes –
T2738 12196-12200 CD denotes 19.2
T2737 12191-12195 CD denotes 16.7
T2736 12188-12190 IN denotes of
T2735 12175-12187 NNS denotes temperatures
T2734 12161-12174 JJ denotes unrecoverable
T2733 12158-12160 IN denotes to
T2732 12149-12157 VBD denotes declined
T2731 12136-12148 NNS denotes temperatures
T2730 12129-12135 JJ denotes Rectal
T2729 12128-12238 sentence denotes Rectal temperatures declined to unrecoverable temperatures of 16.7–19.2 °C in three of the five MCAD−/− mice.
T2728 12127-12128 . denotes .
T2727 12126-12127 -RRB- denotes )
T2726 12123-12124 SYM denotes =
T2725 12121-12122 NN denotes n
T2724 12125-12126 CD denotes 4
T2723 12120-12121 -LRB- denotes (
T2722 12113-12114 SYM denotes +
T2721 12112-12113 HYPH denotes /
T2720 12111-12112 SYM denotes +
T2719 12115-12119 NNS denotes mice
T2717 12103-12106 IN denotes for
T2716 12100-12102 NN denotes °C
T2715 12097-12099 CD denotes 35
T2714 12092-12096 IN denotes with
T2713 12083-12091 VBN denotes compared
T2712 12080-12082 IN denotes as
T2711 12077-12079 NN denotes °C
T2710 12072-12076 CD denotes 23.4
T2709 12066-12067 -RRB- denotes )
T2708 12063-12064 SYM denotes =
T2707 12061-12062 NN denotes n
T2706 12065-12066 CD denotes 5
T2705 12060-12061 -LRB- denotes (
T2704 12053-12054 SYM denotes −
T2703 12052-12053 HYPH denotes /
T2702 12051-12052 SYM denotes −
T2701 12055-12059 NNS denotes mice
T2700 12047-12051 NN denotes MCAD
T2699 12044-12046 IN denotes of
T2698 12025-12031 JJ denotes rectal
T2697 12017-12024 JJ denotes average
T2696 12032-12043 NN denotes temperature
T2695 12013-12016 DT denotes the
T2694 12011-12013 , denotes ,
T2693 11997-12001 NN denotes cold
T2692 12002-12011 NN denotes challenge
T2691 11993-11996 DT denotes the
T2690 11990-11992 IN denotes of
T2689 11988-11989 NN denotes h
T2688 11986-11987 CD denotes 1
T2687 12068-12071 VBD denotes was
T2686 11980-11985 IN denotes After
T2685 11979-12128 sentence denotes After 1 h of the cold challenge, the average rectal temperature of MCAD−/− mice (n = 5) was 23.4 °C as compared with 35 °C for MCAD+/+ mice (n = 4).
T2684 11978-11979 . denotes .
T2683 11968-11978 NNS denotes fatalities
T2682 11965-11967 IN denotes in
T2681 11958-11964 VB denotes result
T2680 11955-11957 TO denotes to
T2679 11948-11954 RB denotes enough
T2678 11941-11947 JJ denotes severe
T2677 11936-11940 DT denotes some
T2676 11934-11936 , denotes ,
T2675 11928-11934 NN denotes stress
T2674 11923-11927 NN denotes cold
T2673 11920-11922 IN denotes of
T2672 11907-11912 JJ denotes short
T2671 11913-11919 NN denotes period
T2670 11902-11906 DT denotes this
T2669 11895-11901 IN denotes within
T2668 11869-11882 RB denotes significantly
T2667 11864-11868 VBD denotes were
T2666 11883-11894 VBN denotes compromised
T2665 11857-11858 SYM denotes −
T2664 11856-11857 HYPH denotes /
T2663 11855-11856 SYM denotes −
T2662 11851-11855 NN denotes MCAD
T2661 11859-11863 NNS denotes mice
T2660 11847-11850 DT denotes The
T2659 11846-11979 sentence denotes The MCAD−/− mice were significantly compromised within this short period of cold stress, some severe enough to result in fatalities.
T2658 11845-11846 . denotes .
T2657 11836-11837 HYPH denotes -
T2656 11837-11838 NN denotes h
T2655 11835-11836 CD denotes 3
T2654 11839-11845 NN denotes period
T2653 11833-11834 DT denotes a
T2652 11829-11832 IN denotes for
T2651 11817-11828 NN denotes environment
T2650 11814-11816 NN denotes °C
T2649 11812-11813 CD denotes 4
T2648 11809-11811 IN denotes in
T2647 11802-11808 VBN denotes placed
T2646 11798-11801 CC denotes and
T2645 11796-11797 NN denotes h
T2644 11793-11795 CD denotes 18
T2643 11789-11792 IN denotes for
T2642 11777-11781 VBD denotes were
T2641 11772-11776 NNS denotes mice
T2640 11770-11772 , denotes ,
T2639 11764-11770 NN denotes stress
T2638 11759-11763 NN denotes cold
T2637 11756-11758 IN denotes of
T2636 11748-11755 NNS denotes effects
T2635 11744-11747 DT denotes the
T2634 11782-11788 VBN denotes fasted
T2633 11734-11743 VB denotes determine
T2632 11731-11733 TO denotes To
T2631 11730-11846 sentence denotes To determine the effects of cold stress, mice were fasted for 18 h and placed in 4 °C environment for a 3-h period.
T2630 11729-11730 . denotes .
T2629 11728-11729 -RRB- denotes )
T2628 11727-11728 CD denotes 1
T2627 11721-11726 NN denotes Table
T2626 11720-11721 -LRB- denotes (
T2625 11713-11714 SYM denotes +
T2624 11712-11713 HYPH denotes /
T2623 11711-11712 SYM denotes +
T2622 11715-11719 NNS denotes mice
T2621 11707-11711 NN denotes MCAD
T2620 11704-11706 IN denotes to
T2619 11695-11703 VBN denotes compared
T2618 11692-11694 IN denotes as
T2617 11690-11692 , denotes ,
T2616 11679-11690 JJ denotes significant
T2615 11668-11674 NN denotes result
T2614 11660-11667 DT denotes neither
T2613 11675-11678 VBD denotes was
T2612 11651-11659 IN denotes although
T2611 11633-11638 JJ denotes fatty
T2610 11639-11643 NN denotes acid
T2609 11628-11632 JJ denotes free
T2608 11622-11627 NN denotes serum
T2607 11644-11650 NNS denotes levels
T2606 11613-11621 VBN denotes elevated
T2605 11609-11612 CC denotes and
T2604 11595-11600 NN denotes serum
T2603 11601-11608 NN denotes glucose
T2602 11589-11594 JJR denotes lower
T2601 11579-11588 VBD denotes displayed
T2600 11572-11573 SYM denotes −
T2599 11571-11572 HYPH denotes /
T2598 11570-11571 SYM denotes −
T2597 11574-11578 NNS denotes mice
T2596 11566-11570 NN denotes MCAD
T2595 11565-11730 sentence denotes MCAD−/− mice displayed lower serum glucose and elevated serum free fatty acid levels although neither result was significant, as compared to MCAD+/+ mice (Table 1).
T2594 11564-11565 . denotes .
T3178 13777-13778 . denotes .
T3177 13762-13763 CD denotes 1
T3176 13761-13762 : denotes :
T3175 13764-13777 NN denotes acylcarnitine
T3174 13758-13761 NN denotes C10
T3173 13751-13753 PRP denotes it
T3172 13745-13750 NN denotes mouse
T3171 13741-13744 DT denotes the
T3170 13738-13740 IN denotes in
T3169 13754-13757 VBD denotes was
T3168 13730-13737 IN denotes whereas
T3167 13728-13730 , denotes ,
T3166 13722-13728 NNS denotes humans
T3165 13719-13721 IN denotes in
T3164 13705-13718 NN denotes acylcarnitine
T3163 13702-13704 NN denotes C8
T3162 13681-13692 JJ denotes predominant
T3161 13693-13697 NN denotes peak
T3160 13677-13680 DT denotes the
T3159 13675-13677 , denotes ,
T3158 13668-13675 RB denotes however
T3157 13666-13667 : denotes ;
T3156 13655-13661 NN denotes mutant
T3155 13662-13666 NNS denotes mice
T3154 13651-13654 DT denotes the
T3153 13647-13650 VBD denotes did
T3152 13644-13646 IN denotes as
T3151 13642-13644 , denotes ,
T3150 13628-13642 NNS denotes acylcarnitines
T3149 13626-13627 CD denotes 1
T3148 13625-13626 : denotes :
T3147 13622-13625 NN denotes C10
T3146 13618-13621 CC denotes and
T3145 13616-13618 , denotes ,
T3144 13614-13616 NN denotes C8
T3143 13612-13614 , denotes ,
T3142 13610-13612 NN denotes C6
T3141 13607-13609 IN denotes of
T3140 13600-13606 NNS denotes levels
T3139 13591-13599 VBN denotes elevated
T3138 13586-13590 IN denotes with
T3137 13698-13701 VBD denotes was
T3136 13578-13585 VBP denotes present
T3135 13569-13577 NNS denotes patients
T3134 13563-13568 JJ denotes Human
T3133 13562-13778 sentence denotes Human patients present with elevated levels of C6, C8, and C10:1 acylcarnitines, as did the mutant mice; however, the predominant peak was C8 acylcarnitine in humans, whereas in the mouse it was C10:1 acylcarnitine.
T3132 13561-13562 . denotes .
T3131 13560-13561 -RRB- denotes )
T3130 13558-13560 CD denotes 4C
T3129 13551-13557 NN denotes Figure
T3128 13550-13551 -LRB- denotes (
T3127 13530-13531 HYPH denotes -
T3126 13531-13540 JJ denotes deficient
T3125 13526-13530 NN denotes MCAD
T3124 13541-13549 NNS denotes patients
T3123 13520-13525 JJ denotes human
T3122 13517-13519 IN denotes of
T3121 13511-13516 DT denotes those
T3120 13506-13510 IN denotes from
T3119 13496-13505 JJ denotes different
T3118 13485-13486 SYM denotes −
T3117 13484-13485 HYPH denotes /
T3116 13483-13484 SYM denotes −
T3115 13479-13483 NN denotes MCAD
T3114 13487-13491 NNS denotes mice
T3113 13475-13478 DT denotes the
T3112 13472-13474 IN denotes of
T3111 13449-13462 NN denotes acylcarnitine
T3110 13463-13471 NNS denotes profiles
T3109 13445-13448 DT denotes the
T3108 13443-13445 , denotes ,
T3107 13492-13495 VBP denotes are
T3106 13436-13443 RB denotes However
T3105 13435-13562 sentence denotes However, the acylcarnitine profiles of the MCAD−/− mice are different from those of human MCAD-deficient patients (Figure 4C).
T3104 13434-13435 . denotes .
T3103 13433-13434 -RRB- denotes )
T3102 13431-13433 CD denotes 4B
T3101 13424-13430 NN denotes Figure
T3100 13423-13424 -LRB- denotes (
T3099 13417-13422 NN denotes serum
T3098 13414-13416 IN denotes in
T3097 13411-13413 IN denotes as
T3096 13389-13402 NN denotes acylcarnitine
T3095 13381-13388 JJ denotes similar
T3094 13403-13410 NN denotes pattern
T3093 13379-13380 DT denotes a
T3092 13370-13378 VBD denotes revealed
T3091 13361-13369 NN denotes analysis
T3090 13347-13360 NN denotes acylcarnitine
T3089 13342-13346 NN denotes Bile
T3088 13341-13435 sentence denotes Bile acylcarnitine analysis revealed a similar acylcarnitine pattern as in serum (Figure 4B).
T3087 13340-13341 . denotes .
T3086 13339-13340 -RRB- denotes )
T3085 13337-13339 CD denotes 4A
T3084 13330-13336 NN denotes Figure
T3083 13329-13330 -LRB- denotes (
T3082 13307-13320 NN denotes acylcarnitine
T3081 13321-13328 NN denotes profile
T3080 13303-13306 DT denotes the
T3079 13300-13302 IN denotes in
T2593 11556-11564 NN denotes analysis
T2592 11553-11555 IN denotes to
T2591 11547-11552 JJ denotes prior
T2590 11545-11546 NN denotes h
T2589 11542-11544 CD denotes 24
T2588 11538-11541 IN denotes for
T2587 11526-11530 VBD denotes were
T2586 11521-11525 PRP denotes they
T2585 11519-11521 , denotes ,
T2584 11515-11519 NNS denotes mice
T2583 11504-11505 HYPH denotes -
T2582 11505-11514 JJ denotes deficient
T2581 11500-11504 NN denotes MCAD
T2580 11497-11499 IN denotes on
T2579 11489-11496 NN denotes fasting
T2578 11486-11488 IN denotes of
T2577 11471-11477 NN denotes stress
T2576 11478-11485 NNS denotes effects
T2575 11467-11470 DT denotes the
T2574 11459-11466 VB denotes examine
T2573 11456-11458 TO denotes to
T2572 11450-11455 NN denotes order
T2571 11531-11537 VBN denotes fasted
T2570 11447-11449 IN denotes In
T2569 11446-11565 sentence denotes In order to examine the stress effects of fasting on MCAD-deficient mice, they were fasted for 24 h prior to analysis.
T2568 11435-11446 NN denotes Intolerance
T2470 11415-11416 . denotes .
T2469 11408-11415 NNS denotes matings
T2468 11395-11407 JJ denotes heterozygous
T2467 11390-11394 IN denotes from
T2466 11380-11389 NNS denotes pedigrees
T2465 11376-11379 DT denotes the
T2464 11367-11375 VB denotes evaluate
T2463 11364-11366 TO denotes to
T2462 11357-11363 JJ denotes unable
T2461 11349-11351 PRP denotes we
T2460 11347-11349 , denotes ,
T2459 11352-11356 VBD denotes were
T2458 11343-11347 RB denotes Thus
T2457 11342-11416 sentence denotes Thus, we were unable to evaluate the pedigrees from heterozygous matings.
T2456 11341-11342 . denotes .
T2455 11332-11333 SYM denotes −
T2454 11331-11332 HYPH denotes /
T2453 11330-11331 SYM denotes −
T2452 11326-11330 NN denotes MCAD
T2451 11324-11325 SYM denotes ×
T2450 11322-11323 SYM denotes −
T2449 11321-11322 HYPH denotes /
T2448 11320-11321 SYM denotes −
T2447 11334-11341 NNS denotes matings
T2446 11316-11320 NN denotes MCAD
T2445 11311-11315 IN denotes with
T2444 11301-11302 SYM denotes +
T2443 11300-11301 HYPH denotes /
T2442 11299-11300 SYM denotes +
T2441 11295-11299 NN denotes MCAD
T2440 11293-11294 SYM denotes ×
T2439 11291-11292 SYM denotes +
T2438 11290-11291 HYPH denotes /
T2436 11303-11310 NNS denotes matings
T2435 11285-11289 NN denotes MCAD
T2434 11272-11276 RB denotes only
T2433 11266-11271 MD denotes could
T2432 11263-11265 PRP denotes we
T2431 11261-11263 , denotes ,
T2430 11243-11252 NN denotes insertion
T2429 11253-11261 NN denotes mutation
T2428 11239-11242 DT denotes the
T2427 11236-11238 IN denotes to
T2426 11232-11235 IN denotes due
T2425 11223-11231 NN denotes analysis
T2424 11213-11222 JJ denotes molecular
T2423 11210-11212 IN denotes by
T2422 11192-11204 JJ denotes heterozygous
T2421 11190-11191 SYM denotes −
T2420 11189-11190 HYPH denotes /
T2419 11188-11189 SYM denotes +
T2418 11184-11188 NN denotes MCAD
T2417 11205-11209 NNS denotes pups
T2416 11180-11183 DT denotes the
T2415 11175-11179 IN denotes from
T2414 11165-11166 SYM denotes −
T2413 11164-11165 HYPH denotes /
T2412 11163-11164 SYM denotes −
T2411 11159-11163 NN denotes MCAD
T2410 11167-11174 NNS denotes mutants
T2409 11155-11158 DT denotes the
T2408 11140-11154 VBG denotes distinguishing
T2407 11137-11139 IN denotes of
T2406 11126-11136 NN denotes difficulty
T2405 11122-11125 DT denotes the
T2404 11119-11121 IN denotes of
T2403 11277-11284 VB denotes compare
T2402 11111-11118 IN denotes Because
T2401 11110-11342 sentence denotes Because of the difficulty of distinguishing the MCAD−/− mutants from the MCAD+/− heterozygous pups by molecular analysis due to the insertion mutation, we could only compare MCAD+/+ × MCAD+/+ matings with MCAD−/− × MCAD−/− matings.
T2400 11109-11110 . denotes .
T2399 11108-11109 SYM denotes +
T2398 11107-11108 HYPH denotes /
T2397 11106-11107 SYM denotes +
T2396 11102-11106 NN denotes MCAD
T2395 11097-11101 IN denotes than
T2394 11085-11096 NN denotes hypothermia
T2393 11082-11084 IN denotes to
T2392 11076-11081 JJ denotes prone
T2391 11071-11075 RBR denotes more
T2390 11064-11070 RB denotes likely
T2389 11060-11063 VBP denotes are
T2388 11055-11059 PRP denotes They
T2387 11054-11110 sentence denotes They are likely more prone to hypothermia than MCAD+/+.
T2386 11053-11054 . denotes .
T2385 11046-11053 NNS denotes reasons
T2384 11038-11045 JJ denotes unknown
T2383 11034-11037 IN denotes for
T2382 11027-11028 SYM denotes +
T2381 11026-11027 HYPH denotes /
T2380 11025-11026 SYM denotes +
T2379 11029-11033 NNS denotes pups
T2378 11021-11025 NN denotes MCAD
T2377 11016-11020 IN denotes than
T2376 11005-11015 RB denotes frequently
T2375 11000-11004 RBR denotes more
T2374 10986-10989 VBP denotes are
T2373 10990-10999 VBN denotes abandoned
T2372 10979-10980 SYM denotes −
T2371 10978-10979 HYPH denotes /
T2370 10977-10978 SYM denotes −
T2369 10973-10977 NN denotes MCAD
T2368 10981-10985 NNS denotes pups
T2367 10969-10972 DT denotes The
T2366 10968-11054 sentence denotes The MCAD−/− pups are abandoned more frequently than MCAD+/+ pups for unknown reasons.
T2365 10967-10968 . denotes .
T2364 10955-10967 JJ denotes undetermined
T2363 10942-10946 NN denotes loss
T2362 10933-10941 JJ denotes neonatal
T2361 10930-10932 IN denotes of
T2360 10947-10954 VBZ denotes remains
T2359 10920-10929 NN denotes mechanism
T2358 10916-10919 DT denotes The
T2357 10915-10968 sentence denotes The mechanism of neonatal loss remains undetermined.
T2356 10914-10915 . denotes .
T2355 10913-10914 -RRB- denotes )
T2354 10912-10913 CD denotes 1
T2353 10906-10911 NN denotes Table
T2352 10905-10906 -LRB- denotes (
T2351 10898-10899 SYM denotes +
T2350 10897-10898 HYPH denotes /
T2349 10896-10897 SYM denotes +
T2348 10900-10904 NNS denotes mice
T2347 10892-10896 NN denotes MCAD
T2346 10889-10891 IN denotes of
T2345 10887-10888 NN denotes %
T2344 10885-10887 CD denotes 98
T2343 10882-10884 IN denotes to
T2342 10873-10881 VBN denotes compared
T2341 10870-10872 IN denotes as
T2340 10862-10869 NN denotes weaning
T2339 10859-10861 IN denotes to
T2338 10843-10844 SYM denotes −
T2337 10842-10843 HYPH denotes /
T2336 10841-10842 SYM denotes −
T2335 10845-10849 NNS denotes mice
T2334 10837-10841 NN denotes MCAD
T2333 10834-10836 IN denotes of
T2332 10830-10832 CD denotes 41
T2331 10832-10833 NN denotes %
T2330 10825-10829 RB denotes only
T2329 10823-10825 , denotes ,
T2328 10817-10818 SYM denotes −
T2327 10816-10817 HYPH denotes /
T2326 10815-10816 SYM denotes −
T2325 10811-10815 NN denotes MCAD
T2324 10807-10810 CC denotes and
T2323 10805-10806 SYM denotes +
T2322 10804-10805 HYPH denotes /
T2321 10803-10804 SYM denotes +
T2320 10819-10823 NNS denotes mice
T2319 10799-10803 NN denotes MCAD
T2318 10794-10798 IN denotes from
T2803 12435-12436 . denotes .
T2802 12433-12435 NN denotes °C
T2801 12428-12432 CD denotes 33.6
T2800 12425-12427 IN denotes of
T2799 12406-12412 JJ denotes rectal
T2798 12398-12405 JJ denotes average
T2797 12413-12424 NN denotes temperature
T2796 12395-12397 DT denotes an
T2795 12390-12394 IN denotes with
T2794 12383-12389 VBG denotes ending
T2793 12381-12383 , denotes ,
T2792 12370-12374 NN denotes cold
T2791 12367-12368 HYPH denotes -
T2790 12368-12369 NN denotes h
T2789 12366-12367 CD denotes 3
T2788 12375-12381 NN denotes stress
T2787 12362-12365 DT denotes the
T2786 12346-12347 SYM denotes +
T2785 12345-12346 HYPH denotes /
T2784 12344-12345 SYM denotes +
T2317 10850-10858 VBD denotes survived
T2316 10784-10788 VBD denotes were
T2315 10779-10783 NNS denotes pups
T2314 10776-10778 IN denotes of
T2313 10768-10775 NNS denotes numbers
T2312 10762-10767 JJ denotes equal
T2311 10789-10793 VBN denotes born
T2310 10753-10761 IN denotes Although
T2309 10752-10915 sentence denotes Although equal numbers of pups were born from MCAD+/+ and MCAD−/− mice, only 41% of MCAD−/− mice survived to weaning as compared to 98% of MCAD+/+ mice (Table 1).
T2308 10751-10752 . denotes .
T2307 10745-10746 SYM denotes −
T2306 10744-10745 HYPH denotes /
T2305 10743-10744 SYM denotes −
T2304 10747-10751 NNS denotes pups
T2303 10739-10743 NN denotes MCAD
T2302 10736-10738 IN denotes in
T2301 10726-10729 VBD denotes was
T2300 10730-10735 VBN denotes noted
T2299 10707-10715 JJ denotes neonatal
T2298 10716-10725 NN denotes mortality
T2297 10695-10706 JJ denotes Significant
T2296 10694-10752 sentence denotes Significant neonatal mortality was noted in MCAD−/− pups.
T2295 10688-10694 NNS denotes Deaths
T2294 10679-10687 JJ denotes Neonatal
T2567 11430-11434 NN denotes Cold
T2566 11426-11429 CC denotes and
T2565 11418-11425 NN denotes Fasting
T2154 10272-10281 NN denotes palmitoyl
T2166 10329-10341 RB denotes respectively
T2167 10341-10343 , denotes ,
T2168 10343-10345 IN denotes in
T2169 10346-10350 NN denotes MCAD
T2170 10354-10358 NNS denotes mice
T2171 10350-10351 SYM denotes −
T2172 10351-10352 HYPH denotes /
T2173 10352-10353 SYM denotes −
T2174 10359-10361 IN denotes as
T2175 10362-10370 VBN denotes compared
T43 0-6 JJ denotes Medium
T44 7-12 NN denotes Chain
T45 0-68 sentence denotes Medium-Chain Acyl-CoA Dehydrogenase Deficiency in Gene-Targeted Mice
T46 6-7 HYPH denotes -
T47 22-35 NN denotes Dehydrogenase
T48 13-17 NN denotes Acyl
T2176 10371-10373 IN denotes to
T2177 10374-10378 NN denotes MCAD
T2178 10382-10390 NNS denotes controls
T2179 10378-10379 SYM denotes +
T2180 10379-10380 HYPH denotes /
T2181 10380-10381 SYM denotes +
T2182 10390-10391 . denotes .
T49 18-21 NN denotes CoA
T50 17-18 HYPH denotes -
T51 36-46 NN denotes Deficiency
T52 47-49 IN denotes in
T53 50-54 NN denotes Gene
T54 55-63 VBN denotes Targeted
T55 54-55 HYPH denotes -
T56 64-68 NNS denotes Mice
T57 68-255 sentence denotes MCAD Gene Disruption in the Mouse Abstract Medium-chain acyl-CoA dehydrogenase (MCAD) deficiency is the most common inherited disorder of mitochondrial fatty acid β-oxidation in humans.
T58 69-119 JJ denotes MCAD Gene Disruption in the Mouse Abstract Medium
T59 120-125 NN denotes chain
T60 119-120 HYPH denotes -
T61 135-148 NN denotes dehydrogenase
T62 126-130 NN denotes acyl
T63 131-134 NN denotes CoA
T64 130-131 HYPH denotes -
T65 156-166 NN denotes deficiency
T66 149-150 -LRB- denotes (
T67 150-154 NN denotes MCAD
T68 154-155 -RRB- denotes )
T69 167-169 VBZ denotes is
T70 170-173 DT denotes the
T71 196-204 NN denotes disorder
T72 174-178 RBS denotes most
T73 179-185 JJ denotes common
T74 186-195 VBN denotes inherited
T75 205-207 IN denotes of
T76 208-221 JJ denotes mitochondrial
T77 235-244 NN denotes oxidation
T122 428-432 NNS denotes mice
T123 420-424 NN denotes MCAD
T124 424-425 SYM denotes −
T125 425-426 HYPH denotes /
T126 426-427 SYM denotes −
T127 433-442 VBD denotes developed
T128 443-445 DT denotes an
T129 454-462 NN denotes aciduria
T130 446-453 JJ denotes organic
T131 463-466 CC denotes and
T132 467-472 JJ denotes fatty
T133 473-478 NN denotes liver
T134 478-480 , denotes ,
T135 480-483 CC denotes and
T136 484-490 VBD denotes showed
T137 491-499 JJ denotes profound
T138 505-516 NN denotes intolerance
T139 500-504 NN denotes cold
T140 517-519 IN denotes at
T141 520-521 CD denotes 4
T142 522-524 NN denotes °C
T143 525-529 IN denotes with
T144 530-535 JJ denotes prior
T145 536-543 NN denotes fasting
T146 543-544 . denotes .
T147 544-641 sentence denotes The sporadic cardiac lesions seen in MCAD−/− mice have not been reported in human MCAD patients.
T148 545-548 DT denotes The
T149 566-573 NNS denotes lesions
T150 549-557 JJ denotes sporadic
T151 558-565 JJ denotes cardiac
T6836 27184-27193 VBN denotes performed
T6839 27200-27203 NN denotes gas
T6838 27194-27199 VBG denotes using
T6837 27179-27183 VBD denotes were
T6835 27170-27178 NNS denotes analyses
T6834 27157-27164 JJ denotes organic
T6833 27165-27169 NN denotes acid
T6832 27151-27156 NN denotes Urine
T6831 27150-27472 sentence denotes Urine organic acid analyses were performed using gas chromatography-mass spectroscopy as previously described [22,30], except tetracosane (C24; Sigma, St. Louis, Missouri, United States) was used as the internal standard, and quantitative determinations were made based on comparison with synthetic calibration standards.
T6830 27149-27150 . denotes .
T6829 27147-27149 NN denotes nm
T6828 27143-27146 CD denotes 550
T6827 27140-27142 IN denotes at
T6826 27132-27139 NN denotes density
T6825 27124-27131 JJ denotes optical
T6824 27121-27123 IN denotes of
T6823 27109-27120 NN denotes measurement
T6822 27105-27108 IN denotes for
T6821 27087-27097 NN denotes microplate
T6820 27098-27104 NN denotes reader
T6819 27085-27086 DT denotes a
T6818 27082-27084 IN denotes of
T6817 27078-27081 NN denotes use
T6816 27074-27077 CC denotes and
T6815 27072-27073 -RRB- denotes )
T6814 27067-27069 CD denotes 10
T5010 20912-20914 DT denotes an
T5009 20910-20912 , denotes ,
T5008 21097-21103 VBN denotes cloned
T5007 20906-20910 RB denotes Next
T5006 20905-21148 sentence denotes Next, an 8-kb Acadm genomic fragment spanning exons 8, 9, and 10 and flanking intron sequences, originally obtained from a Lambda FIXII 129Sv mouse genomic library [24,25], was directionally cloned into the NotI and XhoI sites of pGEM11Zf(+).
T5005 20904-20905 . denotes .
T5004 20895-20896 -RRB- denotes )
T5003 20894-20895 SYM denotes +
T5002 20893-20894 -LRB- denotes (
T5001 20885-20893 NN denotes pGEMl1Zf
T5000 20897-20904 NN denotes plasmid
T4999 20881-20884 DT denotes the
T4998 20878-20880 IN denotes of
T4997 20867-20877 NN denotes polylinker
T4996 20863-20866 DT denotes the
T4995 20856-20862 IN denotes within
T4994 20845-20850 NN denotes EcoRI
T4993 20851-20855 NN denotes site
T4992 20841-20844 DT denotes the
T4991 20831-20840 VBD denotes destroyed
T4990 20827-20830 CC denotes and
T4989 20820-20826 NN denotes vector
T4988 20816-20819 DT denotes the
T4987 20790-20795 NN denotes blunt
T4986 20796-20800 NNS denotes ends
T4985 20786-20789 DT denotes the
T4984 20783-20785 IN denotes of
T4983 20801-20815 VBD denotes recircularized
T4982 20774-20782 NN denotes ligation
T4981 20763-20773 JJ denotes Subsequent
T4980 20762-20905 sentence denotes Subsequent ligation of the blunt ends recircularized the vector and destroyed the EcoRI site within the polylinker of the pGEMl1Zf(+) plasmid.
T4979 20761-20762 . denotes .
T4978 20755-20761 NN denotes enzyme
T4977 20748-20754 NN denotes Klenow
T4976 20743-20747 IN denotes with
T4975 20731-20735 VBD denotes were
R74 T187 T186 cc and,episodes
R75 T188 T186 conj mortality,episodes
R76 T189 T183 prep in,patterns
R1 T78 T79 amod fatty,acid
R2 T79 T77 compound acid,oxidation
R3 T80 T77 compound β,oxidation
R4 T81 T77 punct -,oxidation
R5 T82 T69 prep in,is
R6 T83 T82 pobj humans,in
R7 T84 T69 punct .,is
R8 T86 T87 aux To,understand
R9 T87 T89 advcl understand,developed
R10 T88 T87 advmod better,understand
R11 T90 T91 det the,pathogenesis
R12 T91 T87 dobj pathogenesis,understand
R13 T92 T91 prep of,pathogenesis
R14 T93 T94 det this,disease
R15 T94 T92 pobj disease,of
R16 T95 T89 punct ", ",developed
R17 T96 T89 nsubj we,developed
R18 T97 T98 det a,model
R19 T98 T89 dobj model,developed
R20 T99 T98 compound mouse,model
R21 T100 T98 prep for,model
R22 T101 T102 compound MCAD,deficiency
R23 T102 T100 pobj deficiency,for
R24 T103 T102 punct (,deficiency
R25 T104 T102 appos MCAD,deficiency
R26 T105 T104 punct −,MCAD
R27 T106 T104 punct /,MCAD
R28 T107 T104 punct −,MCAD
R29 T108 T89 punct ),developed
R30 T109 T89 prep by,developed
R31 T110 T111 compound gene,targeting
R32 T111 T109 pobj targeting,by
R33 T112 T111 prep in,targeting
R34 T113 T114 amod embryonic,stem
R35 T114 T115 nmod stem,cells
R36 T115 T112 pobj cells,in
R37 T116 T114 punct (,stem
R38 T117 T114 appos ES,stem
R39 T118 T115 punct ),cells
R40 T119 T89 punct .,developed
R41 T121 T122 det The,mice
R42 T153 T149 acl seen,lesions
R43 T154 T153 prep in,seen
R44 T155 T156 nmod MCAD,mice
R45 T156 T154 pobj mice,in
R46 T157 T155 punct −,MCAD
R47 T158 T155 punct /,MCAD
R48 T159 T155 punct −,MCAD
R49 T160 T152 aux have,reported
R50 T161 T152 neg not,reported
R51 T162 T152 auxpass been,reported
R52 T163 T152 prep in,reported
R53 T164 T165 amod human,patients
R54 T165 T163 pobj patients,in
R55 T166 T165 compound MCAD,patients
R56 T167 T152 punct .,reported
R57 T169 T170 expl There,was
R58 T171 T172 amod significant,mortality
R59 T172 T170 attr mortality,was
R60 T173 T172 amod neonatal,mortality
R61 T174 T172 prep of,mortality
R62 T175 T176 nmod MCAD,pups
R63 T176 T174 pobj pups,of
R64 T177 T175 punct −,MCAD
R65 T178 T175 punct /,MCAD
R66 T179 T175 punct −,MCAD
R67 T180 T172 acl demonstrating,mortality
R68 T181 T180 dobj similarities,demonstrating
R69 T182 T181 prep to,similarities
R70 T183 T182 pobj patterns,to
R71 T184 T183 prep of,patterns
R72 T185 T186 amod clinical,episodes
R73 T186 T184 pobj episodes,of
R77 T190 T191 npadvmod MCAD,deficient
R78 T191 T193 amod deficient,patients
R79 T192 T191 punct -,deficient
R80 T193 T189 pobj patients,in
R81 T194 T170 punct .,was
R82 T196 T197 det The,mouse
R83 T197 T201 nsubj mouse,reproduced
R84 T198 T199 npadvmod MCAD,deficient
R85 T199 T197 amod deficient,mouse
R86 T200 T199 punct -,deficient
R87 T202 T203 amod important,aspects
R88 T203 T201 dobj aspects,reproduced
R89 T204 T203 prep of,aspects
R90 T205 T206 amod human,deficiency
R91 T206 T204 pobj deficiency,of
R92 T207 T206 compound MCAD,deficiency
R93 T208 T201 cc and,reproduced
R94 T209 T201 conj is,reproduced
R95 T210 T211 det a,model
R96 T211 T209 attr model,is
R97 T212 T211 amod valuable,model
R98 T213 T211 prep for,model
R99 T214 T215 amod further,analysis
R100 T215 T213 pobj analysis,for
R101 T216 T215 prep of,analysis
R102 T217 T218 det the,roles
R103 T218 T216 pobj roles,of
R104 T219 T218 prep of,roles
R105 T220 T221 amod fatty,acid
R106 T221 T222 compound acid,oxidation
R107 T222 T219 pobj oxidation,of
R108 T223 T218 cc and,roles
R109 T224 T218 conj pathogenesis,roles
R110 T225 T224 prep of,pathogenesis
R111 T226 T227 amod human,diseases
R112 T227 T225 pobj diseases,of
R113 T228 T227 acl involving,diseases
R114 T229 T230 amod fatty,acid
R115 T230 T231 compound acid,oxidation
R116 T231 T228 dobj oxidation,involving
R117 T232 T201 punct .,reproduced
R122 T43 T44 amod Medium,Chain
R123 T44 T47 compound Chain,Dehydrogenase
R124 T46 T44 punct -,Chain
R125 T47 T51 compound Dehydrogenase,Deficiency
R126 T48 T49 compound Acyl,CoA
R127 T49 T47 compound CoA,Dehydrogenase
R128 T50 T49 punct -,CoA
R129 T52 T51 prep in,Deficiency
R130 T53 T54 npadvmod Gene,Targeted
R131 T54 T56 amod Targeted,Mice
R132 T55 T54 punct -,Targeted
R133 T56 T52 pobj Mice,in
R134 T58 T59 amod "MCAD Gene Disruption in the Mouse Abstract Medium",chain
R135 T59 T61 nmod chain,dehydrogenase
R136 T60 T59 punct -,chain
R137 T61 T65 nmod dehydrogenase,deficiency
R138 T62 T63 nmod acyl,CoA
R139 T63 T61 nmod CoA,dehydrogenase
R140 T64 T63 punct -,CoA
R141 T65 T69 nsubj deficiency,is
R142 T66 T61 punct (,dehydrogenase
R143 T67 T61 appos MCAD,dehydrogenase
R144 T68 T65 punct ),deficiency
R145 T70 T71 det the,disorder
R146 T71 T69 attr disorder,is
R147 T72 T73 advmod most,common
R148 T73 T71 amod common,disorder
R149 T74 T71 amod inherited,disorder
R150 T75 T71 prep of,disorder
R151 T76 T77 amod mitochondrial,oxidation
R152 T77 T75 pobj oxidation,of
R153 T122 T127 nsubj mice,developed
R154 T123 T122 nmod MCAD,mice
R155 T124 T123 punct −,MCAD
R156 T125 T123 punct /,MCAD
R157 T126 T123 punct −,MCAD
R158 T128 T129 det an,aciduria
R159 T129 T127 dobj aciduria,developed
R160 T130 T129 amod organic,aciduria
R161 T131 T129 cc and,aciduria
R162 T132 T133 amod fatty,liver
R163 T133 T129 conj liver,aciduria
R164 T134 T127 punct ", ",developed
R165 T135 T127 cc and,developed
R166 T136 T127 conj showed,developed
R167 T137 T138 amod profound,intolerance
R168 T138 T136 dobj intolerance,showed
R169 T139 T138 compound cold,intolerance
R170 T140 T136 prep at,showed
R171 T141 T142 nummod 4,°C
R172 T142 T140 pobj °C,at
R173 T143 T136 prep with,showed
R174 T144 T145 amod prior,fasting
R175 T145 T143 pobj fasting,with
R176 T146 T127 punct .,developed
R177 T148 T149 det The,lesions
R178 T149 T152 nsubjpass lesions,reported
R179 T150 T149 amod sporadic,lesions
R180 T151 T149 amod cardiac,lesions
R181 T373 T374 amod Mitochondrial,oxidation
R182 T374 T377 nsubj oxidation,provides
R183 T375 T374 compound β,oxidation
R184 T376 T374 punct -,oxidation
R185 T378 T374 prep of,oxidation
R186 T379 T380 amod fatty,acids
R187 T380 T378 pobj acids,of
R188 T381 T377 dobj energy,provides
R189 T382 T377 punct ", ",provides
R190 T383 T384 advmod especially,during
R191 T384 T377 prep during,provides
R192 T385 T386 compound fasting,conditions
R193 T386 T384 pobj conditions,during
R194 T387 T377 punct .,provides
R195 T389 T390 amod Fatty,acid
R196 T390 T391 compound acid,oxidation
R197 T391 T392 nsubj oxidation,occurs
R198 T393 T392 prep in,occurs
R199 T394 T393 pobj mitochondria,in
R200 T395 T392 cc and,occurs
R201 T396 T392 conj consists,occurs
R202 T397 T396 prep of,consists
R203 T398 T399 det a,circuit
R204 T399 T397 pobj circuit,of
R205 T400 T399 amod repeating,circuit
R206 T401 T399 prep of,circuit
R207 T402 T403 nummod four,steps
R208 T403 T401 pobj steps,of
R209 T404 T403 amod sequential,steps
R210 T405 T392 punct .,occurs
R211 T407 T408 expl There,are
R212 T409 T410 nummod four,dehydrogenases
R213 T410 T408 attr dehydrogenases,are
R214 T411 T412 amod straight,chain
R215 T412 T410 compound chain,dehydrogenases
R216 T413 T412 punct -,chain
R217 T414 T415 compound acyl,CoA
R218 T415 T410 compound CoA,dehydrogenases
R219 T416 T415 punct -,CoA
R220 T417 T410 acl involved,dehydrogenases
R221 T418 T417 prep in,involved
R222 T419 T420 det the,step
R223 T420 T418 pobj step,in
R224 T421 T420 amod initial,step
R225 T422 T408 punct .,are
R226 T424 T425 amod Medium,chain
R227 T425 T427 compound chain,dehydrogenase
R228 T426 T425 punct -,chain
R229 T427 T431 nsubj dehydrogenase,is
R230 T428 T429 compound acyl,CoA
R231 T429 T427 compound CoA,dehydrogenase
R232 T430 T429 punct -,CoA
R233 T432 T427 punct (,dehydrogenase
R234 T433 T427 appos MCAD,dehydrogenase
R235 T434 T427 punct ),dehydrogenase
R236 T435 T436 punct (,is
R237 T436 T427 parataxis is,dehydrogenase
R238 T437 T438 det the,gene
R239 T438 T436 nsubj gene,is
R240 T439 T438 compound mouse,gene
R241 T440 T436 attr Acadm,is
R242 T441 T436 punct ", ",is
R243 T442 T443 mark whereas,is
R244 T443 T436 advcl is,is
R245 T444 T445 det the,protein
R246 T445 T443 nsubj protein,is
R247 T446 T443 attr MCAD,is
R248 T447 T436 punct ),is
R249 T448 T431 punct ", ",is
R250 T449 T431 advmod specifically,is
R251 T450 T431 punct ", ",is
R252 T451 T431 acomp responsible,is
R253 T452 T451 prep for,responsible
R254 T453 T452 pcomp catalyzing,for
R255 T454 T455 det the,dehydrogenation
R256 T455 T453 dobj dehydrogenation,catalyzing
R257 T456 T455 prep of,dehydrogenation
R258 T457 T458 amod medium,chain
R259 T458 T460 nmod chain,length
R260 T459 T458 punct -,chain
R261 T460 T461 nmod length,thioesters
R262 T461 T456 pobj thioesters,of
R263 T462 T463 punct (,C12
R264 T463 T460 parataxis C12,length
R265 T464 T463 nmod C6,C12
R266 T465 T463 punct –,C12
R267 T466 T463 punct ),C12
R268 T467 T468 amod fatty,acid
R269 T468 T461 compound acid,thioesters
R270 T469 T470 punct [,1
R271 T470 T431 parataxis 1,is
R272 T471 T470 punct ],1
R273 T472 T431 punct .,is
R274 T474 T475 nsubjpass Acadm,transcribed
R275 T476 T475 auxpass is,transcribed
R276 T477 T475 prep in,transcribed
R277 T478 T479 det the,nucleus
R278 T479 T477 pobj nucleus,in
R279 T480 T475 punct ", ",transcribed
R280 T481 T475 conj translated,transcribed
R281 T482 T481 prep in,translated
R282 T483 T484 det the,cytosol
R283 T484 T482 pobj cytosol,in
R284 T485 T481 punct ", ",translated
R285 T486 T481 cc and,translated
R286 T487 T481 conj translocated,translated
R287 T488 T487 prep into,translocated
R288 T489 T490 det the,matrix
R289 T490 T488 pobj matrix,into
R290 T491 T490 amod mitochondrial,matrix
R291 T492 T493 punct [,2
R292 T493 T487 parataxis 2,translocated
R293 T494 T495 punct –,4
R294 T495 T493 prep 4,2
R295 T496 T493 punct ],2
R296 T497 T475 punct .,transcribed
R297 T499 T500 mark Once,inside
R298 T500 T501 advcl inside,assembled
R299 T502 T503 det the,matrix
R300 T503 T500 pobj matrix,inside
R301 T504 T503 amod mitochondrial,matrix
R302 T505 T501 punct ", ",assembled
R303 T506 T507 det the,monomers
R304 T507 T501 nsubjpass monomers,assembled
R305 T508 T507 compound MCAD,monomers
R306 T509 T501 auxpass are,assembled
R307 T510 T501 prep into,assembled
R308 T511 T510 pobj homotetramers,into
R309 T512 T513 aux to,gain
R310 T513 T501 advcl gain,assembled
R311 T514 T515 amod enzymatic,activity
R312 T515 T513 dobj activity,gain
R313 T516 T517 punct [,4
R314 T517 T501 parataxis 4,assembled
R315 T518 T517 punct ],4
R316 T519 T501 punct .,assembled
R317 T521 T522 compound MCAD,activity
R318 T522 T523 nsubj activity,is
R319 T524 T523 acomp essential,is
R320 T525 T524 prep for,essential
R321 T526 T527 amod complete,oxidation
R322 T527 T525 pobj oxidation,for
R323 T528 T529 amod fatty,acid
R324 T529 T527 compound acid,oxidation
R325 T530 T523 punct .,is
R326 T532 T533 amod Inherited,deficiency
R327 T533 T535 nsubj deficiency,exists
R328 T534 T533 compound MCAD,deficiency
R329 T536 T535 prep in,exists
R330 T537 T536 pobj humans,in
R331 T538 T535 prep as,exists
R332 T539 T540 det an,disorder
R333 T540 T538 pobj disorder,as
R334 T541 T540 amod autosomal,disorder
R335 T542 T540 amod recessive,disorder
R336 T543 T535 punct .,exists
R337 T545 T546 compound MCAD,deficiency
R338 T546 T547 nsubjpass deficiency,described
R339 T548 T547 auxpass was,described
R340 T549 T547 advmod first,described
R341 T550 T547 prep in,described
R342 T551 T550 pobj 1982,in
R343 T552 T553 punct —,1983
R344 T553 T551 prep 1983,1982
R345 T554 T555 punct [,5
R346 T555 T547 parataxis 5,described
R347 T556 T557 punct –,7
R348 T557 T555 prep 7,5
R349 T558 T555 punct ],5
R350 T559 T547 cc and,described
R351 T560 T561 aux has,described
R352 T561 T547 conj described,described
R353 T562 T561 auxpass been,described
R354 T563 T561 prep in,described
R355 T564 T565 amod numerous,patients
R356 T565 T563 pobj patients,in
R357 T566 T567 punct [,1
R358 T567 T561 parataxis 1,described
R359 T568 T567 punct ",",1
R360 T569 T567 appos 8,1
R361 T570 T571 punct –,11
R362 T571 T569 prep 11,8
R363 T572 T567 punct ],1
R364 T573 T547 punct .,described
R365 T575 T576 det The,frequency
R366 T576 T578 nsubjpass frequency,estimated
R367 T577 T576 compound carrier,frequency
R368 T579 T576 prep in,frequency
R369 T580 T581 det the,population
R370 T581 T579 pobj population,in
R371 T582 T581 amod Caucasian,population
R372 T583 T578 aux has,estimated
R373 T584 T578 auxpass been,estimated
R374 T585 T586 aux to,be
R375 T586 T578 xcomp be,estimated
R376 T587 T588 quantmod between,80
R377 T588 T586 attr 80,be
R378 T589 T588 quantmod 1,80
R379 T590 T588 quantmod in,80
R380 T591 T588 quantmod 50,80
R381 T592 T588 quantmod to,80
R382 T593 T586 prep with,be
R383 T594 T595 det an,incidence
R384 T595 T593 pobj incidence,with
R385 T596 T595 prep of,incidence
R386 T597 T598 amod clinical,disease
R387 T598 T596 pobj disease,of
R388 T599 T598 acl expected,disease
R389 T600 T599 prep at,expected
R390 T601 T602 quantmod around,1
R391 T602 T603 nummod 1,"15,000"
R392 T603 T600 pobj "15,000",at
R393 T604 T602 quantmod in,1
R394 T605 T606 punct [,12
R395 T606 T586 parataxis 12,be
R396 T607 T606 nummod 1,12
R397 T608 T606 punct ",",12
R398 T609 T606 nummod 9,12
R399 T610 T606 punct ",",12
R400 T611 T606 punct ],12
R401 T612 T578 punct .,estimated
R402 T614 T615 npadvmod MCAD,deficient
R403 T615 T617 amod deficient,patients
R404 T616 T615 punct -,deficient
R405 T617 T618 nsubj patients,exhibit
R406 T619 T620 amod clinical,episodes
R407 T620 T618 dobj episodes,exhibit
R408 T621 T622 advmod often,associated
R409 T622 T620 acl associated,episodes
R410 T623 T622 prep with,associated
R411 T624 T623 pobj fasting,with
R412 T625 T618 punct .,exhibit
R413 T627 T628 nsubj Patients,manifest
R414 T629 T628 dobj disease,manifest
R415 T630 T631 advmod usually,during
R416 T631 T628 prep during,manifest
R417 T632 T633 det the,years
R418 T633 T631 pobj years,during
R419 T634 T633 amod first,years
R420 T635 T633 nummod two,years
R421 T636 T633 prep of,years
R422 T637 T636 pobj life,of
R423 T638 T628 punct .,manifest
R424 T640 T641 nsubj Symptoms,include
R425 T642 T643 amod hypoketotic,hypoglycemia
R426 T643 T641 dobj hypoglycemia,include
R427 T644 T643 cc and,hypoglycemia
R428 T645 T646 npadvmod Reye,like
R429 T646 T648 amod like,episodes
R430 T647 T646 punct -,like
R431 T648 T643 conj episodes,hypoglycemia
R432 T649 T650 punct [,1
R433 T650 T641 parataxis 1,include
R434 T651 T650 punct ],1
R435 T652 T641 punct .,include
R436 T654 T655 nsubjpass It,estimated
R437 T656 T655 auxpass is,estimated
R438 T657 T658 mark that,die
R439 T658 T655 ccomp die,estimated
R440 T659 T660 advmod approximately,59
R441 T660 T661 nummod 59,%
R442 T661 T658 nsubj %,die
R443 T662 T661 prep of,%
R444 T663 T662 pobj patients,of
R445 T664 T663 acl presenting,patients
R446 T665 T664 advmod clinically,presenting
R447 T666 T664 prep between,presenting
R448 T667 T668 quantmod 15,26
R449 T668 T670 nummod 26,mo
R450 T669 T668 quantmod to,26
R451 T670 T666 pobj mo,between
R452 T671 T670 prep of,mo
R453 T672 T671 pobj age,of
R454 T673 T658 prep during,die
R455 T674 T675 poss their,episode
R456 T675 T673 pobj episode,during
R457 T676 T675 amod first,episode
R458 T677 T675 amod clinical,episode
R459 T678 T679 punct [,1
R460 T679 T655 parataxis 1,estimated
R461 T680 T679 punct ],1
R462 T681 T655 punct .,estimated
R463 T683 T684 det The,pathogenesis
R464 T684 T685 nsubjpass pathogenesis,understood
R465 T686 T684 prep of,pathogenesis
R466 T687 T688 det the,range
R467 T688 T686 pobj range,of
R468 T689 T688 amod wide,range
R469 T690 T688 prep of,range
R470 T691 T692 amod metabolic,disturbances
R471 T692 T690 pobj disturbances,of
R472 T693 T692 prep in,disturbances
R473 T694 T695 compound MCAD,deficiency
R474 T695 T693 pobj deficiency,in
R475 T696 T685 auxpass is,understood
R476 T697 T685 advmod poorly,understood
R477 T698 T685 cc and,understood
R478 T699 T700 amod certain,aspects
R479 T700 T701 nsubj aspects,are
R480 T701 T685 conj are,understood
R481 T702 T700 prep of,aspects
R482 T703 T704 compound patient,management
R483 T704 T702 pobj management,of
R484 T705 T701 acomp controversial,are
R485 T706 T701 punct .,are
R486 T708 T709 det An,model
R487 T709 T711 nsubj model,is
R488 T710 T709 compound animal,model
R489 T712 T709 prep for,model
R490 T713 T714 compound MCAD,deficiency
R491 T714 T712 pobj deficiency,for
R492 T715 T711 acomp essential,is
R493 T716 T717 aux to,understand
R494 T717 T711 advcl understand,is
R495 T718 T717 advmod better,understand
R496 T719 T720 det the,pathogenesis
R497 T720 T717 dobj pathogenesis,understand
R498 T721 T720 prep of,pathogenesis
R499 T722 T723 compound MCAD,deficiency
R500 T723 T721 pobj deficiency,of
R501 T724 T717 cc and,understand
R502 T725 T726 aux to,develop
R503 T726 T717 conj develop,understand
R504 T727 T728 amod better,regimens
R505 T728 T726 dobj regimens,develop
R506 T729 T728 compound management,regimens
R507 T730 T728 prep for,regimens
R508 T731 T732 amod human,patients
R509 T732 T730 pobj patients,for
R510 T733 T711 punct .,is
R511 T735 T736 aux To,gain
R512 T736 T737 advcl gain,developed
R513 T738 T739 amod further,insight
R514 T739 T736 dobj insight,gain
R515 T740 T739 prep into,insight
R516 T741 T742 det the,mechanisms
R517 T742 T740 pobj mechanisms,into
R518 T743 T742 prep of,mechanisms
R519 T744 T745 det this,disease
R520 T745 T743 pobj disease,of
R521 T746 T737 punct ", ",developed
R522 T747 T737 nsubj we,developed
R523 T748 T749 det a,model
R524 T749 T737 dobj model,developed
R525 T750 T749 compound mouse,model
R526 T751 T749 prep of,model
R527 T752 T753 compound MCAD,deficiency
R528 T753 T751 pobj deficiency,of
R529 T754 T737 prep by,developed
R530 T755 T756 compound gene,targeting
R531 T756 T754 pobj targeting,by
R532 T757 T756 prep in,targeting
R533 T758 T759 amod embryonic,stem
R534 T759 T760 nmod stem,cells
R535 T760 T757 pobj cells,in
R536 T761 T759 punct (,stem
R537 T762 T759 appos ES,stem
R538 T763 T760 punct ),cells
R539 T764 T765 punct (,14
R540 T765 T737 parataxis 14,developed
R541 T766 T765 prep for,14
R542 T767 T766 pobj reviews,for
R543 T768 T765 punct [,14
R544 T769 T765 nummod 13,14
R545 T770 T765 punct ",",14
R546 T771 T765 punct ],14
R547 T772 T765 punct ),14
R548 T773 T737 punct .,developed
R549 T775 T776 det The,mice
R550 T776 T778 nsubj mice,had
R551 T777 T776 compound mutant,mice
R552 T779 T780 amod many,features
R553 T780 T778 dobj features,had
R554 T781 T780 amod relevant,features
R555 T782 T780 amod characteristic,features
R556 T783 T782 prep of,characteristic
R557 T784 T785 det the,disease
R558 T785 T783 pobj disease,of
R559 T786 T780 acl found,features
R560 T787 T786 prep in,found
R561 T788 T789 amod human,patients
R562 T789 T787 pobj patients,in
R563 T790 T791 npadvmod MCAD,deficient
R564 T791 T789 amod deficient,patients
R565 T792 T791 punct -,deficient
R566 T793 T780 punct ", ",features
R567 T794 T780 cc along,features
R568 T795 T794 dep with,along
R569 T796 T797 det some,findings
R570 T797 T780 conj findings,features
R571 T798 T797 amod unexpected,findings
R572 T799 T778 punct .,had
R579 T964 T965 compound Gene,Targeting
R580 T966 T965 cc and,Targeting
R581 T967 T965 conj Generation,Targeting
R582 T968 T967 prep of,Generation
R583 T969 T970 npadvmod MCAD,Deficient
R584 T970 T972 amod Deficient,Mice
R585 T971 T970 punct -,Deficient
R586 T972 T968 pobj Mice,of
R587 T974 T975 compound MCAD,vector
R588 T975 T977 nsubjpass vector,designed
R589 T976 T975 compound insertion,vector
R590 T978 T975 punct (,vector
R591 T979 T980 compound MCAD,IV2
R592 T980 T975 appos IV2,vector
R593 T981 T977 punct ),designed
R594 T982 T977 auxpass was,designed
R595 T983 T984 aux to,undergo
R596 T984 T977 advcl undergo,designed
R597 T985 T986 compound gap,repair
R598 T986 T984 dobj repair,undergo
R599 T987 T986 prep of,repair
R600 T988 T989 det the,region
R601 T989 T987 pobj region,of
R602 T990 T991 nummod 1.3,kb
R603 T991 T989 nmod kb,region
R604 T992 T991 punct -,kb
R605 T993 T989 amod deleted,region
R606 T994 T984 prep upon,undergo
R607 T995 T996 amod homologous,recombination
R608 T996 T994 pobj recombination,upon
R609 T997 T984 prep in,undergo
R610 T998 T999 nmod 129P2,cells
R611 T999 T997 pobj cells,in
R612 T1000 T1001 punct (,OlaHsd
R613 T1001 T999 parataxis OlaHsd,cells
R614 T1002 T1001 compound 129P2,OlaHsd
R615 T1003 T1001 punct /,OlaHsd
R616 T1004 T1001 punct ),OlaHsd
R617 T1005 T999 compound ES,cells
R618 T1006 T999 appos E14,cells
R619 T1007 T1006 punct –,E14
R620 T1008 T1006 nummod 1,E14
R621 T1009 T977 punct .,designed
R622 T1011 T1012 amod Correct,targeting
R623 T1012 T1013 nsubj targeting,resulted
R624 T1014 T1012 prep of,targeting
R625 T1015 T1016 det the,locus
R626 T1016 T1014 pobj locus,of
R627 T1017 T1016 compound MCAD,locus
R628 T1018 T1013 prep in,resulted
R629 T1019 T1020 det a,duplication
R630 T1020 T1018 pobj duplication,in
R631 T1021 T1020 prep of,duplication
R632 T1022 T1023 nmod exons,8
R633 T1023 T1021 pobj 8,of
R634 T1024 T1023 punct ", ",8
R635 T1025 T1023 conj 9,8
R636 T1026 T1025 punct ", ",9
R637 T1027 T1025 cc and,9
R638 T1028 T1025 conj 10,9
R639 T1029 T1020 cc and,duplication
R640 T1030 T1020 conj integration,duplication
R641 T1031 T1030 prep of,integration
R642 T1032 T1033 amod flanking,plasmid
R643 T1033 T1031 pobj plasmid,of
R644 T1034 T1033 cc and,plasmid
R645 T1035 T1036 compound Neo,sequences
R646 T1036 T1033 conj sequences,plasmid
R647 T1037 T1038 punct (,Figure
R648 T1038 T1013 parataxis Figure,resulted
R649 T1039 T1038 nummod 1A,Figure
R650 T1040 T1038 punct ),Figure
R651 T1041 T1013 punct .,resulted
R652 T1043 T1044 det The,vector
R653 T1044 T1046 nsubjpass vector,designed
R654 T1045 T1044 compound insertion,vector
R655 T1047 T1046 auxpass was,designed
R656 T1048 T1049 aux to,duplicate
R657 T1049 T1046 advcl duplicate,designed
R658 T1050 T1051 nmod exon,8
R659 T1051 T1049 dobj 8,duplicate
R660 T1052 T1051 punct ", ",8
R661 T1053 T1051 conj 9,8
R662 T1054 T1053 punct ", ",9
R663 T1055 T1053 cc and,9
R664 T1056 T1053 conj 10,9
R665 T1057 T1049 prep at,duplicate
R666 T1058 T1059 det the,locus
R667 T1059 T1057 pobj locus,at
R668 T1060 T1059 compound MCAD,locus
R669 T1061 T1046 punct .,designed
R670 T1063 T1064 nsubj Translation,results
R671 T1065 T1063 prep of,Translation
R672 T1066 T1067 det the,region
R674 T1068 T1067 amod duplicated,region
R690 T1084 T1083 compound MCAD,monomer
R691 T1085 T1064 punct .,results
R692 T1087 T1088 advmod Specifically,arises
R693 T1089 T1088 punct ", ",arises
R694 T1090 T1091 det the,codon
R695 T1091 T1088 nsubj codon,arises
R696 T1092 T1091 amod first,codon
R697 T1093 T1091 amod premature,codon
R698 T1094 T1091 compound stop,codon
R699 T1095 T1088 prep after,arises
R700 T1096 T1095 pobj translation,after
R701 T1097 T1096 prep of,translation
R702 T1098 T1099 advmod only,seven
R703 T1099 T1100 nummod seven,acids
R704 T1100 T1097 pobj acids,of
R705 T1101 T1100 compound amino,acids
R706 T1102 T1100 prep from,acids
R707 T1103 T1104 det the,exon
R708 T1104 T1102 pobj exon,from
R709 T1105 T1104 amod duplicated,exon
R710 T1106 T1104 nummod 8,exon
R711 T1107 T1088 punct .,arises
R712 T1109 T1110 det The,monomer
R713 T1110 T1113 nsubj monomer,missing
R714 T1111 T1110 amod resulting,monomer
R715 T1112 T1110 compound MCAD,monomer
R716 T1114 T1113 aux is,missing
R717 T1115 T1116 det the,helixes
R718 T1116 T1113 dobj helixes,missing
R719 T1117 T1118 npadvmod C,terminal
R720 T1118 T1120 amod terminal,domain
R721 T1119 T1118 punct -,terminal
R722 T1120 T1116 compound domain,helixes
R723 T1121 T1116 compound α,helixes
R724 T1122 T1116 punct -,helixes
R725 T1123 T1124 dep that,are
R726 T1124 T1116 relcl are,helixes
R727 T1125 T1124 acomp responsible,are
R728 T1126 T1125 prep for,responsible
R729 T1127 T1126 pcomp making,for
R730 T1128 T1129 compound intersubunit,contacts
R731 T1129 T1127 dobj contacts,making
R732 T1130 T1131 aux to,generate
R733 T1131 T1127 advcl generate,making
R734 T1132 T1133 det the,homotetramer
R735 T1133 T1131 dobj homotetramer,generate
R736 T1134 T1133 amod functional,homotetramer
R737 T1135 T1133 compound MCAD,homotetramer
R738 T1136 T1113 punct .,missing
R775 T1177 T1163 punct ", ",probe
R776 T1178 T1163 acl hybridized,probe
R777 T1179 T1178 prep to,hybridized
R778 T1180 T1181 det a,band
R779 T1181 T1179 pobj band,to
R780 T1182 T1183 nummod 13.2,kb
R781 T1183 T1181 compound kb,band
R782 T1184 T1183 punct -,kb
R783 T1185 T1178 prep in,hybridized
R784 T1186 T1185 pobj addition,in
R785 T1187 T1186 prep to,addition
R786 T1188 T1189 det the,band
R787 T1189 T1187 pobj band,to
R788 T1190 T1191 nummod 3.1,kb
R789 T1191 T1189 nmod kb,band
R790 T1192 T1191 punct -,kb
R791 T1193 T1189 amod endogenous,band
R792 T1194 T1189 acl indicating,band
R793 T1195 T1196 amod targeted,insertion
R794 T1196 T1194 dobj insertion,indicating
R795 T1197 T1196 prep of,insertion
R796 T1198 T1199 det the,vector
R797 T1199 T1197 pobj vector,of
R798 T1200 T1196 prep at,insertion
R799 T1201 T1202 det the,locus
R800 T1202 T1200 pobj locus,at
R801 T1203 T1202 compound Acadm,locus
R802 T1204 T1205 punct (,Figure
R803 T1205 T1161 parataxis Figure,used
R804 T1206 T1205 nummod 1B,Figure
R805 T1207 T1205 punct ),Figure
R806 T1208 T1161 punct .,used
R807 T1210 T1211 advmod Correctly,targeted
R808 T1211 T1212 amod targeted,clones
R809 T1212 T1215 nsubjpass clones,microinjected
R810 T1213 T1214 compound ES,cell
R811 T1214 T1212 compound cell,clones
R812 T1216 T1215 auxpass were,microinjected
R813 T1217 T1215 prep into,microinjected
R814 T1218 T1219 nmod B6,blastocysts
R815 T1219 T1217 pobj blastocysts,into
R816 T1220 T1219 punct (,blastocysts
R817 T1221 T1222 nmod C57BL,6NTac
R818 T1222 T1219 nmod 6NTac,blastocysts
R819 T1223 T1222 punct /,6NTac
R820 T1224 T1219 punct ),blastocysts
R821 T1225 T1226 aux to,generate
R822 T1226 T1215 advcl generate,microinjected
R823 T1227 T1228 amod chimeric,mice
R824 T1228 T1226 dobj mice,generate
R825 T1229 T1215 punct .,microinjected
R826 T1231 T1232 amod Chimeric,mice
R827 T1232 T1233 nsubjpass mice,backcrossed
R828 T1234 T1233 auxpass were,backcrossed
R829 T1235 T1233 prep to,backcrossed
R830 T1236 T1237 preconj both,129P2
R831 T1237 T1238 npadvmod 129P2,inbred
R832 T1238 T1241 amod inbred,mice
R833 T1239 T1237 cc and,129P2
R834 T1240 T1237 conj B6,129P2
R835 T1241 T1235 pobj mice,to
R836 T1242 T1243 aux to,produce
R837 T1243 T1233 advcl produce,backcrossed
R838 T1244 T1245 nmod MCAD,mice
R839 T1245 T1243 dobj mice,produce
R840 T1246 T1244 punct +,MCAD
R841 T1247 T1244 punct /,MCAD
R842 T1248 T1244 punct −,MCAD
R843 T1249 T1244 cc and,MCAD
R844 T1250 T1251 advmod eventually,MCAD
R845 T1251 T1244 conj MCAD,MCAD
R846 T1252 T1251 punct −,MCAD
R847 T1253 T1251 punct /,MCAD
R848 T1254 T1251 punct −,MCAD
R849 T1255 T1243 prep on,produce
R850 T1256 T1257 det a,background
R851 T1257 T1255 pobj background,on
R852 T1258 T1257 nmod B6,background
R853 T1259 T1258 punct /,B6
R854 T1260 T1258 nummod 129,B6
R855 T1261 T1257 amod mixed,background
R856 T1262 T1233 punct .,backcrossed
R857 T1264 T1265 det The,studies
R858 T1265 T1266 nsubjpass studies,conducted
R859 T1267 T1265 acl described,studies
R860 T1268 T1267 advmod here,described
R861 T1269 T1266 auxpass were,conducted
R862 T1270 T1266 advmod exclusively,conducted
R863 T1271 T1266 prep on,conducted
R864 T1272 T1273 det the,background
R865 T1273 T1271 pobj background,on
R866 T1274 T1273 nmod B6,background
R867 T1275 T1274 punct /,B6
R868 T1276 T1274 nummod 129,B6
R869 T1277 T1273 amod mixed,background
R870 T1278 T1273 prep compared,background
R871 T1279 T1278 prep with,compared
R872 T1280 T1281 compound littermate,controls
R873 T1281 T1279 pobj controls,with
R874 T1282 T1281 cc or,controls
R875 T1283 T1284 nmod B6,groups
R876 T1284 T1281 conj groups,controls
R877 T1285 T1283 punct /,B6
R878 T1286 T1283 nummod 129,B6
R879 T1287 T1284 compound control,groups
R880 T1288 T1281 acl maintained,controls
R881 T1289 T1288 prep by,maintained
R882 T1290 T1289 pobj intercrosses,by
R883 T1291 T1292 mark as,were
R884 T1292 T1288 advcl were,maintained
R885 T1293 T1294 det the,mutants
R886 T1294 T1292 nsubj mutants,were
R887 T1295 T1266 punct .,conducted
R888 T1297 T1298 csubj Perpetuating,proved
R889 T1299 T1300 det this,mutation
R890 T1300 T1297 dobj mutation,Perpetuating
R891 T1301 T1297 prep as,Perpetuating
R892 T1302 T1303 det a,line
R893 T1303 T1301 pobj line,as
R894 T1304 T1303 amod congenic,line
R895 T1305 T1303 compound mutant,line
R896 T1306 T1303 prep on,line
R897 T1307 T1308 det the,background
R898 T1308 T1306 pobj background,on
R899 T1309 T1308 compound 129P2,background
R900 T1310 T1298 oprd impractical,proved
R901 T1311 T1298 punct .,proved
R902 T1313 T1314 det The,mice
R903 T1314 T1316 nsubj mice,were
R904 T1315 T1314 compound 129P2,mice
R905 T1317 T1318 amod poor,breeders
R906 T1318 T1316 attr breeders,were
R907 T1319 T1316 prep as,were
R908 T1320 T1321 amod wild,types
R909 T1321 T1319 pobj types,as
R910 T1322 T1321 punct -,types
R911 T1323 T1316 punct ", ",were
R912 T1324 T1316 cc and,were
R913 T1325 T1326 advmod when,introduced
R914 T1326 T1327 advcl introduced,lost
R915 T1327 T1316 conj lost,were
R916 T1328 T1327 punct ", ",lost
R917 T1329 T1330 det the,mutation
R918 T1330 T1327 nsubjpass mutation,lost
R919 T1331 T1330 compound Acadm,mutation
R920 T1332 T1327 auxpass was,lost
R921 T1333 T1327 advmod nearly,lost
R922 T1334 T1327 prep on,lost
R923 T1335 T1336 det this,background
R924 T1336 T1334 pobj background,on
R925 T1337 T1327 prep because,lost
R926 T1338 T1337 pcomp of,because
R927 T1339 T1340 det the,rate
R928 T1340 T1337 pobj rate,because
R929 T1341 T1340 amod high,rate
R930 T1342 T1340 prep of,rate
R931 T1343 T1344 amod neonatal,death
R932 T1344 T1342 pobj death,of
R933 T1345 T1327 punct .,lost
R934 T1347 T1348 prep Because,proved
R935 T1349 T1347 pcomp of,Because
R936 T1350 T1351 det the,structure
R937 T1351 T1347 pobj structure,Because
R938 T1352 T1351 amod molecular,structure
R939 T1353 T1351 prep of,structure
R940 T1354 T1355 det the,allele
R941 T1355 T1353 pobj allele,of
R942 T1356 T1355 amod targeted,allele
R943 T1357 T1348 punct ", ",proved
R944 T1358 T1348 nsubj it,proved
R945 T1359 T1360 advmod virtually,impossible
R946 T1360 T1348 oprd impossible,proved
R947 T1361 T1362 aux to,distinguish
R948 T1362 T1348 xcomp distinguish,proved
R949 T1363 T1364 det all,genotypes
R950 T1364 T1362 dobj genotypes,distinguish
R951 T1365 T1364 nummod three,genotypes
R952 T1366 T1364 amod potential,genotypes
R953 T1367 T1348 punct .,proved
R954 T1369 T1370 nsubj We,detect
R955 T1370 T1373 ccomp detect,determined
R956 T1371 T1370 aux could,detect
R957 T1372 T1370 advmod clearly,detect
R958 T1374 T1375 det the,presence
R959 T1375 T1370 dobj presence,detect
R960 T1376 T1375 cc or,presence
R961 T1377 T1375 conj absence,presence
R962 T1378 T1375 prep of,presence
R963 T1379 T1380 det the,allele
R964 T1380 T1378 pobj allele,of
R965 T1381 T1380 amod targeted,allele
R966 T1382 T1373 punct ", ",determined
R967 T1383 T1373 advmod however,determined
R968 T1384 T1373 punct ", ",determined
R969 T1385 T1386 mark whether,was
R970 T1386 T1373 csubjpass was,determined
R971 T1387 T1388 det a,mouse
R972 T1388 T1386 nsubj mouse,was
R973 T1389 T1388 amod particular,mouse
R974 T1390 T1386 attr MCAD,was
R975 T1391 T1390 punct −,MCAD
R976 T1392 T1390 punct /,MCAD
R977 T1393 T1390 punct −,MCAD
R978 T1394 T1390 cc or,MCAD
R979 T1395 T1390 conj MCAD,MCAD
R980 T1396 T1395 punct +,MCAD
R981 T1397 T1395 punct /,MCAD
R982 T1398 T1395 punct −,MCAD
R983 T1399 T1373 aux could,determined
R984 T1400 T1373 neg not,determined
R985 T1401 T1373 auxpass be,determined
R986 T1402 T1373 prep by,determined
R987 T1403 T1404 compound Southern,blot
R988 T1404 T1402 pobj blot,by
R989 T1405 T1404 cc or,blot
R990 T1406 T1404 conj PCR,blot
R991 T1407 T1404 prep of,blot
R992 T1408 T1409 amod genomic,DNA
R993 T1409 T1407 pobj DNA,of
R994 T1410 T1373 punct .,determined
R995 T1412 T1413 advmod Ultimately,ascertained
R996 T1414 T1415 nmod MCAD,mice
R997 T1415 T1413 nsubjpass mice,ascertained
R998 T1416 T1414 punct −,MCAD
R999 T1417 T1414 punct /,MCAD
R1000 T1418 T1414 punct −,MCAD
R1001 T1419 T1413 auxpass were,ascertained
R1002 T1420 T1413 prep by,ascertained
R1003 T1421 T1422 compound immunoblot,analysis
R1004 T1422 T1420 pobj analysis,by
R1005 T1423 T1422 prep of,analysis
R1006 T1424 T1423 pobj offspring,of
R1007 T1425 T1422 prep with,analysis
R1008 T1426 T1427 amod subsequent,perpetuation
R1009 T1427 T1425 pobj perpetuation,with
R1010 T1428 T1427 prep of,perpetuation
R1011 T1429 T1430 nmod MCAD,mice
R1012 T1430 T1428 pobj mice,of
R1013 T1431 T1429 punct −,MCAD
R1014 T1432 T1429 punct /,MCAD
R1015 T1433 T1429 punct −,MCAD
R1016 T1434 T1429 cc and,MCAD
R1017 T1435 T1429 conj MCAD,MCAD
R1018 T1436 T1435 punct +,MCAD
R1019 T1437 T1435 punct /,MCAD
R1020 T1438 T1435 punct +,MCAD
R1021 T1439 T1427 prep as,perpetuation
R1022 T1440 T1441 amod separate,groups
R1023 T1441 T1439 pobj groups,as
R1024 T1442 T1413 punct .,ascertained
R1025 T1606 T1607 compound RNA,Analysis
R1026 T1609 T1610 compound RT,PCR
R1027 T1610 T1612 compound PCR,amplification
R1028 T1611 T1610 punct -,PCR
R1029 T1612 T1613 nsubj amplification,amplified
R1030 T1614 T1612 prep from,amplification
R1031 T1615 T1616 nmod exon,7
R1032 T1616 T1614 pobj 7,from
R1033 T1617 T1616 prep to,7
R1034 T1618 T1617 pobj 11,to
R1035 T1619 T1612 prep from,amplification
R1036 T1620 T1621 amod total,RNA
R1037 T1621 T1619 pobj RNA,from
R1038 T1622 T1621 compound heart,RNA
R1039 T1623 T1624 det the,fragment
R1040 T1624 T1613 dobj fragment,amplified
R1041 T1625 T1624 amod expected,fragment
R1042 T1626 T1627 nummod 600,pair
R1043 T1627 T1624 nmod pair,fragment
R1044 T1628 T1627 punct -,pair
R1045 T1629 T1627 nmod base,pair
R1046 T1630 T1627 punct (,pair
R1047 T1631 T1627 appos bp,pair
R1048 T1632 T1624 punct ),fragment
R1049 T1633 T1624 prep in,fragment
R1050 T1634 T1635 nmod MCAD,mice
R1051 T1635 T1633 pobj mice,in
R1052 T1636 T1634 punct +,MCAD
R1053 T1637 T1634 punct /,MCAD
R1054 T1638 T1634 punct +,MCAD
R1055 T1639 T1634 cc and,MCAD
R1056 T1640 T1634 conj MCAD,MCAD
R1057 T1641 T1640 punct +,MCAD
R1058 T1642 T1640 punct /,MCAD
R1059 T1643 T1640 punct −,MCAD
R1060 T1644 T1624 punct ", ",fragment
R1061 T1645 T1624 cc and,fragment
R1062 T1646 T1647 det a,fragment
R1063 T1647 T1624 conj fragment,fragment
R1064 T1648 T1649 nummod 1.5,kb
R1065 T1649 T1647 compound kb,fragment
R1066 T1650 T1649 punct -,kb
R1067 T1651 T1647 prep in,fragment
R1068 T1652 T1653 nmod MCAD,mice
R1069 T1653 T1651 pobj mice,in
R1070 T1654 T1652 punct −,MCAD
R1071 T1655 T1652 punct /,MCAD
R1072 T1656 T1652 punct −,MCAD
R1073 T1657 T1658 punct (,shown
R1074 T1658 T1613 parataxis shown,amplified
R1075 T1659 T1658 nsubj data,shown
R1076 T1660 T1658 neg not,shown
R1077 T1661 T1658 punct ),shown
R1078 T1662 T1613 punct .,amplified
R1079 T1664 T1665 compound Sequence,analysis
R1080 T1665 T1666 nsubj analysis,revealed
R1081 T1667 T1665 prep of,analysis
R1082 T1668 T1669 det the,fragment
R1083 T1669 T1667 pobj fragment,of
R1084 T1670 T1671 nummod 1.5,kb
R1085 T1671 T1669 compound kb,fragment
R1086 T1672 T1671 punct -,kb
R1087 T1673 T1674 mark that,consisted
R1088 T1674 T1666 ccomp consisted,revealed
R1089 T1675 T1676 det the,fragment
R1090 T1676 T1674 nsubj fragment,consisted
R1091 T1677 T1676 amod amplified,fragment
R1092 T1678 T1674 prep of,consisted
R1093 T1679 T1678 pobj exon,of
R1094 T1680 T1679 nummod 7,exon
R1095 T1681 T1679 prep to,exon
R1096 T1682 T1681 pobj exon,to
R1097 T1683 T1682 nummod 10,exon
R1098 T1684 T1674 prep with,consisted
R1099 T1685 T1686 nummod 280,bp
R1100 T1686 T1684 pobj bp,with
R1101 T1687 T1686 prep of,bp
R1102 T1688 T1689 compound pGEM,sequence
R1103 T1689 T1687 pobj sequence,of
R1104 T1690 T1689 compound plasmid,sequence
R1105 T1691 T1689 acl followed,sequence
R1106 T1692 T1691 agent by,followed
R1107 T1693 T1694 nmod exons,8
R1108 T1694 T1692 pobj 8,by
R1109 T1695 T1696 punct –,11
R1110 T1696 T1694 prep 11,8
R1111 T1697 T1666 punct .,revealed
R1112 T1699 T1700 nsubjpass Some,deleted
R1113 T1701 T1699 prep of,Some
R1114 T1702 T1703 det the,sequences
R1115 T1703 T1701 pobj sequences,of
R1116 T1704 T1703 compound plasmid,sequences
R1117 T1705 T1699 punct ", ",Some
R1118 T1706 T1699 cc along,Some
R1119 T1707 T1706 dep with,along
R1120 T1708 T1709 det the,sequence
R1121 T1709 T1699 conj sequence,Some
R1122 T1710 T1709 compound pPGKNEOpA,sequence
R1123 T1711 T1700 punct ", ",deleted
R1124 T1712 T1700 auxpass were,deleted
R1125 T1713 T1700 prep from,deleted
R1126 T1714 T1715 det this,mRNA
R1127 T1715 T1713 pobj mRNA,from
R1128 T1716 T1715 amod spliced,mRNA
R1129 T1717 T1700 punct .,deleted
R1130 T1719 T1720 compound Northern,blot
R1131 T1720 T1721 compound blot,analysis
R1132 T1721 T1722 nsubj analysis,revealed
R1133 T1723 T1724 nsubjpass Acadm,expressed
R1134 T1724 T1722 advcl expressed,revealed
R1135 T1725 T1724 auxpass was,expressed
R1136 T1726 T1724 advmod normally,expressed
R1137 T1727 T1724 prep in,expressed
R1138 T1728 T1729 det all,tissues
R1139 T1729 T1727 pobj tissues,in
R1140 T1730 T1729 acl analyzed,tissues
R1141 T1731 T1729 prep from,tissues
R1142 T1732 T1733 nmod MCAD,mice
R1143 T1733 T1731 pobj mice,from
R1144 T1734 T1732 punct +,MCAD
R1145 T1735 T1732 punct /,MCAD
R1146 T1736 T1732 punct +,MCAD
R1147 T1737 T1724 prep with,expressed
R1148 T1738 T1739 det the,expression
R1149 T1739 T1742 nsubj expression,occurring
R1150 T1740 T1741 advmod most,robust
R1151 T1741 T1739 amod robust,expression
R1152 T1742 T1737 pcomp occurring,with
R1153 T1743 T1742 prep in,occurring
R1154 T1744 T1745 amod brown,fat
R1155 T1745 T1743 pobj fat,in
R1156 T1746 T1745 punct ", ",fat
R1157 T1747 T1745 conj kidney,fat
R1158 T1748 T1747 punct ", ",kidney
R1159 T1749 T1747 conj heart,kidney
R1160 T1750 T1749 punct ", ",heart
R1161 T1751 T1752 amod skeletal,muscle
R1162 T1752 T1749 conj muscle,heart
R1163 T1753 T1752 punct ", ",muscle
R1164 T1754 T1752 cc and,muscle
R1165 T1755 T1752 conj liver,muscle
R1166 T1756 T1724 prep with,expressed
R1167 T1757 T1758 amod minimal,expression
R1168 T1758 T1756 pobj expression,with
R1169 T1759 T1758 prep in,expression
R1170 T1760 T1761 det the,brain
R1171 T1761 T1759 pobj brain,in
R1172 T1762 T1761 punct ", ",brain
R1173 T1763 T1764 amod white,fat
R1174 T1764 T1761 conj fat,brain
R1175 T1765 T1764 punct ", ",fat
R1176 T1766 T1764 cc and,fat
R1177 T1767 T1764 conj testes,fat
R1178 T1768 T1769 punct (,Figure
R1179 T1769 T1722 parataxis Figure,revealed
R1180 T1770 T1769 nummod 2,Figure
R1181 T1771 T1769 punct ),Figure
R1182 T1772 T1722 punct .,revealed
R1183 T1774 T1775 advmod Interestingly,detected
R1184 T1776 T1775 punct ", ",detected
R1185 T1777 T1778 mark although,amplified
R1186 T1778 T1775 advcl amplified,detected
R1187 T1779 T1780 compound RT,PCR
R1188 T1780 T1778 nsubj PCR,amplified
R1189 T1781 T1780 punct -,PCR
R1190 T1782 T1783 det an,transcript
R1191 T1783 T1778 dobj transcript,amplified
R1192 T1784 T1785 advmod incorrectly,spliced
R1193 T1785 T1783 amod spliced,transcript
R1194 T1786 T1783 compound Acadm,transcript
R1195 T1787 T1775 punct ", ",detected
R1196 T1788 T1789 det no,transcripts
R1197 T1789 T1775 nsubjpass transcripts,detected
R1198 T1790 T1789 compound Acadm,transcripts
R1199 T1791 T1775 auxpass were,detected
R1200 T1792 T1775 prep by,detected
R1201 T1793 T1794 compound northern,blot
R1202 T1794 T1795 compound blot,analysis
R1203 T1795 T1792 pobj analysis,by
R1204 T1796 T1775 prep from,detected
R1205 T1797 T1798 nmod MCAD,mice
R1206 T1798 T1796 pobj mice,from
R1207 T1799 T1797 punct −,MCAD
R1208 T1800 T1797 punct /,MCAD
R1209 T1801 T1797 punct −,MCAD
R1210 T1802 T1775 punct .,detected
R1211 T1804 T1805 det These,results
R1212 T1805 T1806 nsubj results,suggest
R1213 T1807 T1806 advmod strongly,suggest
R1214 T1808 T1809 mark that,is
R1215 T1809 T1806 ccomp is,suggest
R1216 T1810 T1811 det the,RNA
R1217 T1811 T1809 nsubj RNA,is
R1218 T1812 T1811 compound mutant,RNA
R1219 T1813 T1809 acomp unstable,is
R1220 T1814 T1809 cc and,is
R1221 T1815 T1809 conj degraded,is
R1222 T1816 T1815 advmod rapidly,degraded
R1223 T1817 T1809 cc or,is
R1224 T1818 T1809 punct ", ",is
R1225 T1819 T1820 advmod alternatively,undergoes
R1226 T1820 T1809 conj undergoes,is
R1227 T1821 T1820 punct ", ",undergoes
R1228 T1822 T1823 npadvmod nonsense,mediated
R1229 T1823 T1824 amod mediated,decay
R1230 T1824 T1820 dobj decay,undergoes
R1231 T1825 T1824 compound RNA,decay
R1232 T1826 T1806 punct .,suggest
R1235 T1979 T1980 compound Liver,Enzyme
R1236 T1980 T1981 compound Enzyme,Analyses
R1237 T1983 T1984 compound Immunoblot,analyses
R1238 T1984 T1985 nsubj analyses,demonstrated
R1239 T1986 T1984 prep of,analyses
R1240 T1987 T1988 compound liver,homogenates
R1241 T1988 T1986 pobj homogenates,of
R1242 T1989 T1984 prep with,analyses
R1243 T1990 T1991 amod anti-MCAD,antisera
R1244 T1991 T1989 pobj antisera,with
R1245 T1992 T1993 mark that,was
R1246 T1993 T1985 ccomp was,demonstrated
R1247 T1994 T1995 det the,monomer
R1248 T1995 T1993 nsubj monomer,was
R1249 T1996 T1997 nummod 42,kDa
R1250 T1997 T1995 compound kDa,monomer
R1251 T1998 T1995 compound MCAD,monomer
R1252 T1999 T1993 acomp present,was
R1253 T2000 T1993 prep in,was
R1254 T2001 T2002 nmod MCAD,mice
R1255 T2002 T2000 pobj mice,in
R1256 T2003 T2001 punct +,MCAD
R1257 T2004 T2001 punct /,MCAD
R1258 T2005 T2001 punct +,MCAD
R1259 T2006 T2000 punct ", ",in
R1260 T2007 T2000 cc but,in
R1261 T2008 T2007 neg not,but
R1262 T2009 T2000 conj in,in
R1263 T2010 T2011 nmod MCAD,mice
R1264 T2011 T2009 pobj mice,in
R1265 T2012 T2010 punct −,MCAD
R1266 T2013 T2010 punct /,MCAD
R1267 T2014 T2010 punct −,MCAD
R1268 T2015 T2016 punct (,Figure
R1269 T2016 T1985 parataxis Figure,demonstrated
R1270 T2017 T2016 nummod 3,Figure
R1271 T2018 T2016 punct ),Figure
R1272 T2019 T1985 punct .,demonstrated
R1273 T2021 T2022 prep As,revealed
R1274 T2023 T2024 det a,analysis
R1275 T2024 T2021 pobj analysis,As
R1276 T2025 T2024 compound control,analysis
R1277 T2026 T2022 punct ", ",revealed
R1278 T2027 T2028 amod anti–short,chain
R1279 T2028 T2030 nmod chain,dehydrogenase
R1280 T2029 T2028 punct -,chain
R1281 T2030 T2034 nmod dehydrogenase,SCAD
R1282 T2031 T2032 nmod acyl,CoA
R1283 T2032 T2030 nmod CoA,dehydrogenase
R1284 T2033 T2032 punct -,CoA
R1285 T2034 T2036 nmod SCAD,antisera
R1286 T2035 T2034 punct (,SCAD
R1287 T2036 T2022 nsubj antisera,revealed
R1288 T2037 T2034 punct ),SCAD
R1289 T2038 T2039 det no,differences
R1290 T2039 T2022 dobj differences,revealed
R1291 T2040 T2039 prep in,differences
R1292 T2041 T2040 pobj levels,in
R1293 T2042 T2041 prep of,levels
R1294 T2043 T2042 pobj expression,of
R1295 T2044 T2043 prep of,expression
R1296 T2045 T2046 compound SCAD,protein
R1297 T2046 T2044 pobj protein,of
R1298 T2047 T2039 prep between,differences
R1299 T2048 T2049 nmod MCAD,mice
R1301 T2050 T2048 punct +,MCAD
R1302 T2051 T2048 punct /,MCAD
R1303 T2052 T2048 punct +,MCAD
R1304 T2053 T2048 cc and,MCAD
R1305 T2054 T2048 conj MCAD,MCAD
R1306 T2055 T2054 punct −,MCAD
R1307 T2056 T2054 punct /,MCAD
R1340 T2091 T2084 cc and,CoA
R1341 T2092 T2093 compound palmitoyl,CoA
R1342 T2093 T2084 conj CoA,CoA
R1343 T2094 T2093 punct -,CoA
R1344 T2095 T2096 punct (,C16
R1345 T2096 T2093 parataxis C16,CoA
R1346 T2097 T2096 punct :,C16
R1347 T2098 T2096 nummod 0,C16
R1348 T2099 T2096 punct ),C16
R1349 T2100 T2084 prep as,CoA
R1350 T2101 T2100 pobj substrates,as
R1351 T2102 T2066 punct .,analyzed
R1352 T2104 T2105 nmod MCAD,mice
R1353 T2105 T2109 nsubj mice,had
R1354 T2106 T2104 punct −,MCAD
R1355 T2107 T2104 punct /,MCAD
R1356 T2108 T2104 punct −,MCAD
R1357 T2110 T2111 det a,reduction
R1358 T2111 T2109 dobj reduction,had
R1359 T2112 T2111 amod significant,reduction
R1360 T2113 T2111 prep in,reduction
R1361 T2114 T2113 pobj ability,in
R1362 T2115 T2116 aux to,dehydrogenate
R1363 T2116 T2114 acl dehydrogenate,ability
R1364 T2117 T2118 compound octanoyl,CoA
R1365 T2118 T2116 dobj CoA,dehydrogenate
R1366 T2119 T2118 punct -,CoA
R1367 T2120 T2111 cc and,reduction
R1368 T2121 T2122 det a,reduction
R1369 T2122 T2111 conj reduction,reduction
R1370 T2123 T2122 amod modest,reduction
R1371 T2124 T2122 prep in,reduction
R1372 T2125 T2124 pobj activity,in
R1373 T2126 T2125 prep toward,activity
R1374 T2127 T2128 compound palmitoyl,CoA
R1375 T2128 T2126 pobj CoA,toward
R1376 T2129 T2128 punct -,CoA
R1377 T2130 T2109 prep compared,had
R1378 T2131 T2130 prep to,compared
R1379 T2132 T2133 nmod MCAD,mice
R1380 T2133 T2131 pobj mice,to
R1381 T2134 T2132 punct +,MCAD
R1382 T2135 T2132 punct /,MCAD
R1383 T2136 T2132 punct +,MCAD
R1384 T2137 T2138 punct (,Table
R1385 T2138 T2109 parataxis Table,had
R1386 T2139 T2138 nummod 1,Table
R1387 T2140 T2138 punct ),Table
R1388 T2141 T2109 punct .,had
R1389 T2143 T2144 advmod Specifically,reduced
R1390 T2145 T2144 punct ", ",reduced
R1391 T2146 T2147 det the,dehydrogenation
R1392 T2147 T2144 nsubjpass dehydrogenation,reduced
R1393 T2148 T2147 prep of,dehydrogenation
R1394 T2149 T2150 nmod octanoyl,CoA
R1395 T2150 T2152 nmod CoA,substrates
R1396 T2151 T2150 punct -,CoA
R1397 T2152 T2148 pobj substrates,of
R1398 T2153 T2150 cc and,CoA
R1399 T2154 T2155 compound palmitoyl,CoA
R1400 T2155 T2150 conj CoA,CoA
R1401 T2156 T2155 punct -,CoA
R1402 T2157 T2144 auxpass were,reduced
R1403 T2158 T2144 prep by,reduced
R1404 T2159 T2160 nummod 75,%
R1405 T2160 T2158 pobj %,by
R1406 T2161 T2158 cc and,by
R1407 T2162 T2158 conj by,by
R1408 T2163 T2164 nummod 30,%
R1409 T2164 T2162 pobj %,by
R1410 T2165 T2144 punct ", ",reduced
R1411 T2166 T2144 advmod respectively,reduced
R1412 T2167 T2144 punct ", ",reduced
R1413 T2168 T2144 prep in,reduced
R1414 T2169 T2170 nmod MCAD,mice
R1415 T2170 T2168 pobj mice,in
R1416 T2171 T2169 punct −,MCAD
R1417 T2172 T2169 punct /,MCAD
R1418 T2173 T2169 punct −,MCAD
R1419 T2174 T2175 mark as,compared
R1420 T2175 T2144 advcl compared,reduced
R1421 T2176 T2175 prep to,compared
R1422 T2177 T2178 nmod MCAD,controls
R1423 T2178 T2176 pobj controls,to
R1424 T2179 T2177 punct +,MCAD
R1425 T2180 T2177 punct /,MCAD
R1426 T2181 T2177 punct +,MCAD
R1427 T2182 T2144 punct .,reduced
R1428 T2294 T2295 amod Neonatal,Deaths
R1429 T2297 T2298 amod Significant,mortality
R1430 T2298 T2300 nsubjpass mortality,noted
R1431 T2299 T2298 amod neonatal,mortality
R1432 T2301 T2300 auxpass was,noted
R1433 T2302 T2300 prep in,noted
R1434 T2303 T2304 nmod MCAD,pups
R1435 T2304 T2302 pobj pups,in
R1436 T2305 T2303 punct −,MCAD
R1437 T2306 T2303 punct /,MCAD
R1438 T2307 T2303 punct −,MCAD
R1439 T2308 T2300 punct .,noted
R1440 T2310 T2311 mark Although,born
R1441 T2311 T2317 advcl born,survived
R1442 T2312 T2313 amod equal,numbers
R1443 T2313 T2311 nsubjpass numbers,born
R1444 T2314 T2313 prep of,numbers
R1445 T2315 T2314 pobj pups,of
R1446 T2316 T2311 auxpass were,born
R1447 T2318 T2311 prep from,born
R1448 T2319 T2320 nmod MCAD,mice
R1449 T2320 T2318 pobj mice,from
R1450 T2321 T2319 punct +,MCAD
R1451 T2322 T2319 punct /,MCAD
R1452 T2323 T2319 punct +,MCAD
R1453 T2324 T2319 cc and,MCAD
R1454 T2325 T2319 conj MCAD,MCAD
R1455 T2326 T2325 punct −,MCAD
R1456 T2327 T2325 punct /,MCAD
R1457 T2328 T2325 punct −,MCAD
R1458 T2329 T2317 punct ", ",survived
R1459 T2330 T2331 advmod only,%
R1460 T2331 T2317 nsubj %,survived
R1461 T2332 T2331 nummod 41,%
R1462 T2333 T2331 prep of,%
R1463 T2334 T2335 nmod MCAD,mice
R1464 T2335 T2333 pobj mice,of
R1465 T2336 T2334 punct −,MCAD
R1466 T2337 T2334 punct /,MCAD
R1467 T2338 T2334 punct −,MCAD
R1468 T2339 T2317 prep to,survived
R1469 T2340 T2339 pobj weaning,to
R1470 T2341 T2342 mark as,compared
R1471 T2342 T2317 advcl compared,survived
R1472 T2343 T2342 prep to,compared
R1473 T2344 T2345 nummod 98,%
R1474 T2345 T2343 pobj %,to
R1475 T2346 T2345 prep of,%
R1476 T2347 T2348 nmod MCAD,mice
R1477 T2348 T2346 pobj mice,of
R1478 T2349 T2347 punct +,MCAD
R1479 T2350 T2347 punct /,MCAD
R1480 T2351 T2347 punct +,MCAD
R1481 T2352 T2353 punct (,Table
R1482 T2353 T2317 parataxis Table,survived
R1483 T2354 T2353 nummod 1,Table
R1484 T2355 T2353 punct ),Table
R1485 T2356 T2317 punct .,survived
R1486 T2358 T2359 det The,mechanism
R1487 T2359 T2360 nsubj mechanism,remains
R1488 T2361 T2359 prep of,mechanism
R1489 T2362 T2363 amod neonatal,loss
R1490 T2363 T2361 pobj loss,of
R1491 T2364 T2360 acomp undetermined,remains
R1492 T2365 T2360 punct .,remains
R1493 T2367 T2368 det The,pups
R1494 T2368 T2373 nsubjpass pups,abandoned
R1495 T2369 T2368 nmod MCAD,pups
R1496 T2370 T2369 punct −,MCAD
R1497 T2371 T2369 punct /,MCAD
R1498 T2372 T2369 punct −,MCAD
R1499 T2374 T2373 auxpass are,abandoned
R1500 T2375 T2376 advmod more,frequently
R1501 T2376 T2373 advmod frequently,abandoned
R1502 T2377 T2376 prep than,frequently
R1503 T2378 T2379 nmod MCAD,pups
R1504 T2379 T2377 pobj pups,than
R1505 T2380 T2378 punct +,MCAD
R1506 T2381 T2378 punct /,MCAD
R1507 T2382 T2378 punct +,MCAD
R1508 T2383 T2373 prep for,abandoned
R1509 T2384 T2385 amod unknown,reasons
R1510 T2385 T2383 pobj reasons,for
R1511 T2386 T2373 punct .,abandoned
R1512 T2388 T2389 nsubj They,are
R1513 T2390 T2389 advmod likely,are
R1514 T2391 T2392 advmod more,prone
R1515 T2392 T2389 acomp prone,are
R1516 T2393 T2392 prep to,prone
R1517 T2394 T2393 pobj hypothermia,to
R1518 T2395 T2392 prep than,prone
R1519 T2396 T2395 pobj MCAD,than
R1520 T2397 T2396 punct +,MCAD
R1521 T2398 T2396 punct /,MCAD
R1522 T2399 T2396 punct +,MCAD
R1523 T2400 T2389 punct .,are
R1524 T2402 T2403 prep Because,compare
R1525 T2404 T2402 pcomp of,Because
R1526 T2405 T2406 det the,difficulty
R1527 T2406 T2402 pobj difficulty,Because
R1528 T2407 T2406 prep of,difficulty
R1529 T2408 T2407 pcomp distinguishing,of
R1530 T2409 T2410 det the,mutants
R1531 T2410 T2408 dobj mutants,distinguishing
R1532 T2411 T2410 nmod MCAD,mutants
R1533 T2412 T2411 punct −,MCAD
R1534 T2413 T2411 punct /,MCAD
R1535 T2414 T2411 punct −,MCAD
R1536 T2415 T2408 prep from,distinguishing
R1537 T2416 T2417 det the,pups
R1538 T2417 T2415 pobj pups,from
R1539 T2418 T2417 nmod MCAD,pups
R1540 T2419 T2418 punct +,MCAD
R1541 T2420 T2418 punct /,MCAD
R1542 T2421 T2418 punct −,MCAD
R1543 T2422 T2417 amod heterozygous,pups
R1544 T2423 T2408 prep by,distinguishing
R1545 T2424 T2425 amod molecular,analysis
R1546 T2425 T2423 pobj analysis,by
R1547 T2426 T2406 prep due,difficulty
R1548 T2427 T2426 pcomp to,due
R1549 T2428 T2429 det the,mutation
R1550 T2429 T2426 pobj mutation,due
R1551 T2430 T2429 compound insertion,mutation
R1552 T2431 T2403 punct ", ",compare
R1553 T2432 T2403 nsubj we,compare
R1554 T2433 T2403 aux could,compare
R1555 T2434 T2403 advmod only,compare
R1556 T2435 T2436 nmod MCAD,matings
R1557 T2436 T2403 dobj matings,compare
R1558 T2437 T2435 punct +,MCAD
R1559 T2438 T2435 punct /,MCAD
R1560 T2439 T2435 punct +,MCAD
R1561 T2440 T2435 punct ×,MCAD
R1562 T2441 T2435 appos MCAD,MCAD
R1563 T2442 T2441 punct +,MCAD
R1564 T2443 T2441 punct /,MCAD
R1565 T2444 T2441 punct +,MCAD
R1566 T2445 T2403 prep with,compare
R1567 T2446 T2447 nmod MCAD,matings
R1568 T2447 T2445 pobj matings,with
R1569 T2448 T2446 punct −,MCAD
R1570 T2449 T2446 punct /,MCAD
R1571 T2450 T2446 punct −,MCAD
R1572 T2451 T2446 punct ×,MCAD
R1573 T2452 T2446 appos MCAD,MCAD
R1574 T2453 T2452 punct −,MCAD
R1575 T2454 T2452 punct /,MCAD
R1576 T2455 T2452 punct −,MCAD
R1577 T2456 T2403 punct .,compare
R1578 T2458 T2459 advmod Thus,were
R1579 T2460 T2459 punct ", ",were
R1580 T2461 T2459 nsubj we,were
R1581 T2462 T2459 acomp unable,were
R1582 T2463 T2464 aux to,evaluate
R1583 T2464 T2462 xcomp evaluate,unable
R1584 T2465 T2466 det the,pedigrees
R1585 T2466 T2464 dobj pedigrees,evaluate
R1586 T2467 T2464 prep from,evaluate
R1587 T2468 T2469 amod heterozygous,matings
R1588 T2469 T2467 pobj matings,from
R1589 T2470 T2459 punct .,were
R1590 T2566 T2565 cc and,Fasting
R1591 T2567 T2568 compound Cold,Intolerance
R1592 T2568 T2565 conj Intolerance,Fasting
R1593 T2570 T2571 prep In,fasted
R1594 T2572 T2570 pobj order,In
R1595 T2573 T2574 aux to,examine
R1596 T2574 T2572 acl examine,order
R1597 T2575 T2576 det the,effects
R1598 T2576 T2574 dobj effects,examine
R1599 T2577 T2576 compound stress,effects
R1600 T2578 T2576 prep of,effects
R1601 T2579 T2578 pobj fasting,of
R1602 T2580 T2576 prep on,effects
R1603 T2581 T2582 npadvmod MCAD,deficient
R1604 T2582 T2584 amod deficient,mice
R1605 T2583 T2582 punct -,deficient
R1606 T2584 T2580 pobj mice,on
R1607 T2585 T2571 punct ", ",fasted
R1608 T2586 T2571 nsubjpass they,fasted
R1609 T2587 T2571 auxpass were,fasted
R1610 T2588 T2571 prep for,fasted
R1611 T2589 T2590 nummod 24,h
R1612 T2590 T2588 pobj h,for
R1613 T2591 T2592 amod prior,to
R1614 T2592 T2571 prep to,fasted
R1615 T2593 T2592 pobj analysis,to
R1616 T2594 T2571 punct .,fasted
R1617 T2596 T2597 nmod MCAD,mice
R1618 T2597 T2601 nsubj mice,displayed
R1619 T2598 T2596 punct −,MCAD
R1620 T2599 T2596 punct /,MCAD
R1621 T2600 T2596 punct −,MCAD
R1622 T2602 T2603 amod lower,glucose
R1623 T2603 T2601 dobj glucose,displayed
R1624 T2604 T2603 compound serum,glucose
R1625 T2605 T2603 cc and,glucose
R1626 T2606 T2607 amod elevated,levels
R1627 T2607 T2603 conj levels,glucose
R1628 T2608 T2609 npadvmod serum,free
R1629 T2609 T2610 amod free,acid
R1630 T2610 T2607 compound acid,levels
R1631 T2611 T2610 amod fatty,acid
R1632 T2612 T2613 mark although,was
R1633 T2613 T2601 advcl was,displayed
R1634 T2614 T2615 det neither,result
R1635 T2615 T2613 nsubj result,was
R1636 T2616 T2613 acomp significant,was
R1637 T2617 T2613 punct ", ",was
R1638 T2618 T2619 mark as,compared
R1639 T2619 T2613 advcl compared,was
R1640 T2620 T2619 prep to,compared
R1641 T2621 T2622 nmod MCAD,mice
R1642 T2622 T2620 pobj mice,to
R1643 T2623 T2621 punct +,MCAD
R1644 T2624 T2621 punct /,MCAD
R1645 T2625 T2621 punct +,MCAD
R1646 T2626 T2627 punct (,Table
R1647 T2627 T2601 parataxis Table,displayed
R1648 T2628 T2627 nummod 1,Table
R1649 T2629 T2627 punct ),Table
R1650 T2630 T2601 punct .,displayed
R1651 T2632 T2633 aux To,determine
R1652 T2633 T2634 advcl determine,fasted
R1653 T2635 T2636 det the,effects
R1654 T2636 T2633 dobj effects,determine
R1655 T2637 T2636 prep of,effects
R1656 T2638 T2639 compound cold,stress
R1657 T2639 T2637 pobj stress,of
R1658 T2640 T2634 punct ", ",fasted
R1659 T2641 T2634 nsubjpass mice,fasted
R1660 T2642 T2634 auxpass were,fasted
R1661 T2643 T2634 prep for,fasted
R1662 T2644 T2645 nummod 18,h
R1663 T2645 T2643 pobj h,for
R1664 T2646 T2634 cc and,fasted
R1665 T2647 T2634 conj placed,fasted
R1666 T2648 T2647 prep in,placed
R1667 T2649 T2650 nummod 4,°C
R1668 T2650 T2651 compound °C,environment
R1669 T2651 T2648 pobj environment,in
R1670 T2652 T2647 prep for,placed
R1671 T2653 T2654 det a,period
R1672 T2654 T2652 pobj period,for
R1673 T2655 T2656 nummod 3,h
R1674 T2656 T2654 compound h,period
R1675 T2657 T2656 punct -,h
R1676 T2658 T2634 punct .,fasted
R1677 T2660 T2661 det The,mice
R1678 T2661 T2666 nsubjpass mice,compromised
R1679 T2662 T2661 nmod MCAD,mice
R1680 T2663 T2662 punct −,MCAD
R1681 T2664 T2662 punct /,MCAD
R1682 T2665 T2662 punct −,MCAD
R1683 T2667 T2666 auxpass were,compromised
R1684 T2668 T2666 advmod significantly,compromised
R1685 T2669 T2666 prep within,compromised
R1686 T2670 T2671 det this,period
R1687 T2671 T2669 pobj period,within
R1688 T2672 T2671 amod short,period
R1689 T2673 T2671 prep of,period
R1690 T2674 T2675 compound cold,stress
R1691 T2675 T2673 pobj stress,of
R1692 T2676 T2666 punct ", ",compromised
R1693 T2677 T2678 nsubj some,severe
R1694 T2678 T2666 amod severe,compromised
R1695 T2679 T2678 advmod enough,severe
R1696 T2680 T2681 aux to,result
R1697 T2681 T2678 xcomp result,severe
R1698 T2682 T2681 prep in,result
R1699 T2683 T2682 pobj fatalities,in
R1700 T2684 T2666 punct .,compromised
R1701 T2686 T2687 prep After,was
R1702 T2688 T2689 nummod 1,h
R1703 T2689 T2686 pobj h,After
R1704 T2690 T2689 prep of,h
R1705 T2691 T2692 det the,challenge
R1706 T2692 T2690 pobj challenge,of
R1707 T2693 T2692 compound cold,challenge
R1708 T2694 T2687 punct ", ",was
R1709 T2695 T2696 det the,temperature
R1710 T2696 T2687 nsubj temperature,was
R1711 T2697 T2696 amod average,temperature
R1712 T2698 T2696 amod rectal,temperature
R1713 T2699 T2696 prep of,temperature
R1714 T2700 T2701 nmod MCAD,mice
R1715 T2701 T2699 pobj mice,of
R1716 T2702 T2700 punct −,MCAD
R1717 T2703 T2700 punct /,MCAD
R1718 T2704 T2700 punct −,MCAD
R1719 T2705 T2706 punct (,5
R1720 T2706 T2701 parataxis 5,mice
R1721 T2707 T2706 nsubj n,5
R1722 T2708 T2706 punct =,5
R1723 T2709 T2706 punct ),5
R1724 T2710 T2711 nummod 23.4,°C
R1725 T2711 T2687 attr °C,was
R1726 T2712 T2713 mark as,compared
R1727 T2713 T2687 advcl compared,was
R1728 T2714 T2713 prep with,compared
R1729 T2715 T2716 nummod 35,°C
R1730 T2716 T2714 pobj °C,with
R1731 T2717 T2716 prep for,°C
R1732 T2718 T2719 nmod MCAD,mice
R1733 T2719 T2717 pobj mice,for
R1734 T2720 T2718 punct +,MCAD
R1735 T2721 T2718 punct /,MCAD
R1736 T2722 T2718 punct +,MCAD
R1737 T2723 T2724 punct (,4
R1738 T2724 T2719 parataxis 4,mice
R1739 T2725 T2724 nsubj n,4
R1740 T2726 T2724 punct =,4
R1741 T2727 T2724 punct ),4
R1742 T2728 T2687 punct .,was
R1743 T2730 T2731 amod Rectal,temperatures
R1744 T2731 T2732 nsubj temperatures,declined
R1745 T2733 T2732 prep to,declined
R1746 T2734 T2735 amod unrecoverable,temperatures
R1747 T2735 T2733 pobj temperatures,to
R1748 T2736 T2735 prep of,temperatures
R1749 T2737 T2738 quantmod 16.7,19.2
R1750 T2738 T2740 nummod 19.2,°C
R1751 T2739 T2738 punct –,19.2
R1752 T2740 T2736 pobj °C,of
R1753 T2741 T2732 prep in,declined
R1754 T2742 T2743 quantmod three,five
R1755 T2743 T2746 nummod five,mice
R1756 T2744 T2743 quantmod of,five
R1757 T2745 T2743 quantmod the,five
R1758 T2746 T2741 pobj mice,in
R1759 T2747 T2746 nmod MCAD,mice
R1760 T2748 T2747 punct −,MCAD
R1761 T2749 T2747 punct /,MCAD
R1762 T2750 T2747 punct −,MCAD
R1763 T2751 T2732 punct .,declined
R1764 T2753 T2754 prep By,averaged
R1765 T2755 T2756 det the,end
R1766 T2756 T2753 pobj end,By
R1767 T2757 T2756 prep of,end
R1768 T2758 T2759 det the,mark
R1769 T2759 T2757 pobj mark,of
R1770 T2760 T2761 nummod 1.5,h
R1771 T2761 T2759 compound h,mark
R1772 T2762 T2761 punct -,h
R1773 T2763 T2754 punct ", ",averaged
R1774 T2764 T2765 det the,mice
R1775 T2765 T2754 nsubj mice,averaged
R1776 T2766 T2765 nummod two,mice
R1777 T2767 T2765 amod surviving,mice
R1778 T2768 T2765 nmod MCAD,mice
R1779 T2769 T2768 punct −,MCAD
R1780 T2770 T2768 punct /,MCAD
R1781 T2771 T2768 punct −,MCAD
R1782 T2772 T2773 nummod 22.7,°C
R1783 T2773 T2754 dobj °C,averaged
R1784 T2774 T2754 punct .,averaged
R1785 T2776 T2777 prep In,survived
R1786 T2778 T2776 pobj contrast,In
R1787 T2779 T2777 punct ", ",survived
R1788 T2780 T2781 det all,mice
R1789 T2781 T2777 nsubj mice,survived
R1790 T2782 T2781 nummod four,mice
R1791 T2783 T2781 nmod MCAD,mice
R1792 T2784 T2783 punct +,MCAD
R1793 T2785 T2783 punct /,MCAD
R1794 T2786 T2783 punct +,MCAD
R1795 T2787 T2788 det the,stress
R1796 T2788 T2777 dobj stress,survived
R1797 T2789 T2790 nummod 3,h
R1798 T2790 T2788 compound h,stress
R1799 T2791 T2790 punct -,h
R1800 T2792 T2788 compound cold,stress
R1801 T2793 T2777 punct ", ",survived
R1802 T2794 T2777 advcl ending,survived
R1803 T2795 T2794 prep with,ending
R1804 T2796 T2797 det an,temperature
R1805 T2797 T2795 pobj temperature,with
R1806 T2798 T2797 amod average,temperature
R1807 T2799 T2797 amod rectal,temperature
R1808 T2800 T2797 prep of,temperature
R1809 T2801 T2802 nummod 33.6,°C
R1810 T2802 T2800 pobj °C,of
R1811 T2803 T2777 punct .,survived
R1812 T2908 T2909 amod Organic,Acid
R1813 T2910 T2909 cc and,Acid
R1814 T2911 T2912 compound Acylcarnitine,Analysis
R1815 T2912 T2909 conj Analysis,Acid
R1816 T2914 T2915 nmod Urine,acid
R1817 T2915 T2917 compound acid,analysis
R1818 T2916 T2915 amod organic,acid
R1819 T2917 T2918 nsubj analysis,revealed
R1820 T2919 T2920 mark that,developed
R1821 T2920 T2918 ccomp developed,revealed
R1822 T2921 T2922 nmod MCAD,mice
R1823 T2922 T2920 nsubj mice,developed
R1824 T2923 T2921 punct −,MCAD
R1825 T2924 T2921 punct /,MCAD
R1826 T2925 T2921 punct −,MCAD
R1827 T2926 T2927 det an,profile
R1828 T2927 T2920 dobj profile,developed
R1829 T2928 T2929 amod organic,acid
R1830 T2929 T2927 compound acid,profile
R1831 T2930 T2927 amod similar,profile
R1832 T2931 T2930 prep to,similar
R1833 T2932 T2933 npadvmod MCAD,deficient
R1834 T2933 T2935 amod deficient,patients
R1835 T2934 T2933 punct -,deficient
R1836 T2935 T2931 pobj patients,to
R1837 T2936 T2935 amod human,patients
R1838 T2937 T2918 punct .,revealed
R1839 T2939 T2940 advmod Specifically,developed
R1840 T2941 T2940 punct ", ",developed
R1841 T2942 T2943 advmod when,fasted
R1842 T2943 T2940 advcl fasted,developed
R1843 T2944 T2943 prep for,fasted
R1844 T2945 T2946 nummod 18,h
R1845 T2946 T2944 pobj h,for
R1846 T2947 T2940 punct ", ",developed
R1847 T2948 T2949 nmod MCAD,mice
R1848 T2949 T2940 nsubj mice,developed
R1849 T2950 T2948 punct −,MCAD
R1850 T2951 T2948 punct /,MCAD
R1851 T2952 T2948 punct −,MCAD
R1852 T2953 T2954 advmod significantly,elevated
R1853 T2954 T2955 amod elevated,concentrations
R1854 T2955 T2940 dobj concentrations,developed
R1855 T2956 T2955 prep of,concentrations
R1856 T2957 T2958 amod adipic,acids
R1857 T2958 T2956 pobj acids,of
R1858 T2959 T2957 punct ", ",adipic
R1859 T2960 T2957 conj suberic,adipic
R1860 T2961 T2960 punct ", ",suberic
R1861 T2962 T2960 cc and,suberic
R1862 T2963 T2960 conj sebacic,suberic
R1863 T2964 T2958 cc and,acids
R1864 T2965 T2958 conj hexanoylglycine,acids
R1865 T2966 T2967 mark as,compared
R1866 T2967 T2940 advcl compared,developed
R1867 T2968 T2967 prep to,compared
R1868 T2969 T2970 nmod MCAD,controls
R1869 T2970 T2968 pobj controls,to
R1870 T2971 T2969 punct +,MCAD
R1871 T2972 T2969 punct /,MCAD
R1872 T2973 T2969 punct +,MCAD
R1873 T2974 T2970 punct ", ",controls
R1874 T2975 T2976 dep which,showed
R1875 T2976 T2970 relcl showed,controls
R1876 T2977 T2976 dobj trace,showed
R1877 T2978 T2977 prep to,trace
R1878 T2979 T2980 det no,amounts
R1879 T2980 T2978 pobj amounts,to
R1880 T2981 T2980 amod detectable,amounts
R1881 T2982 T2977 prep of,trace
R1882 T2983 T2984 det the,acids
R1883 T2984 T2982 pobj acids,of
R1884 T2985 T2984 amod same,acids
R1885 T2986 T2984 amod organic,acids
R1886 T2987 T2988 punct (,Table
R1887 T2988 T2940 parataxis Table,developed
R1888 T2989 T2988 nummod 1,Table
R1889 T2990 T2988 punct ),Table
R1890 T2991 T2940 punct .,developed
R1891 T2993 T2994 amod Adipic,acid
R1892 T2994 T2995 nsubj acid,is
R1893 T2996 T2995 neg not,is
R1894 T2997 T2995 acomp specific,is
R1895 T2998 T2997 prep to,specific
R1896 T2999 T3000 compound MCAD,deficiency
R1897 T3000 T2998 pobj deficiency,to
R1898 T3001 T2995 punct .,is
R1899 T3003 T3004 nsubj We,evaluated
R1900 T3005 T3004 advmod also,evaluated
R1901 T3006 T3007 npadvmod β,hydroxybutyric
R1902 T3007 T3009 amod hydroxybutyric,concentrations
R1903 T3008 T3007 punct -,hydroxybutyric
R1904 T3009 T3004 dobj concentrations,evaluated
R1905 T3010 T3007 cc and,hydroxybutyric
R1906 T3011 T3007 conj acetoacetic,hydroxybutyric
R1907 T3012 T3004 cc and,evaluated
R1908 T3013 T3004 conj found,evaluated
R1909 T3014 T3015 det no,differences
R1910 T3015 T3013 dobj differences,found
R1911 T3016 T3015 amod significant,differences
R1912 T3017 T3015 prep between,differences
R1913 T3018 T3019 compound MCAD,genotypes
R1914 T3019 T3017 pobj genotypes,between
R1915 T3020 T3021 punct (,shown
R1916 T3021 T3013 parataxis shown,found
R1917 T3022 T3021 nsubj data,shown
R1918 T3023 T3021 neg not,shown
R1919 T3024 T3021 punct ),shown
R1920 T3025 T3004 punct .,evaluated
R1921 T3027 T3028 nsubj Comparison,revealed
R1922 T3029 T3027 prep of,Comparison
R1923 T3030 T3031 nmod MCAD,mice
R1924 T3031 T3029 pobj mice,of
R1925 T3032 T3030 punct +,MCAD
R1926 T3033 T3030 punct /,MCAD
R1927 T3034 T3030 punct +,MCAD
R1928 T3035 T3030 cc and,MCAD
R1929 T3036 T3030 conj MCAD,MCAD
R1930 T3037 T3036 punct −,MCAD
R1931 T3038 T3036 punct /,MCAD
R1932 T3039 T3036 punct −,MCAD
R1933 T3040 T3041 det no,differences
R1934 T3041 T3028 dobj differences,revealed
R1935 T3042 T3041 amod significant,differences
R1936 T3043 T3041 prep in,differences
R1937 T3044 T3045 amod total,concentrations
R1938 T3045 T3043 pobj concentrations,in
R1939 T3046 T3045 compound serum,concentrations
R1940 T3047 T3045 compound carnitine,concentrations
R1941 T3048 T3041 prep between,differences
R1942 T3049 T3050 nmod MCAD,mice
R1943 T3050 T3048 pobj mice,between
R1944 T3051 T3049 punct +,MCAD
R1945 T3052 T3049 punct /,MCAD
R1946 T3053 T3049 punct +,MCAD
R1947 T3054 T3049 cc and,MCAD
R1948 T3055 T3049 conj MCAD,MCAD
R1949 T3056 T3055 punct −,MCAD
R1950 T3057 T3055 punct /,MCAD
R1951 T3058 T3055 punct −,MCAD
R1952 T3059 T3028 punct ", ",revealed
R1953 T3060 T3028 cc but,revealed
R1954 T3061 T3062 nmod MCAD,mice
R1955 T3062 T3066 nsubj mice,had
R1956 T3063 T3061 punct −,MCAD
R1957 T3064 T3061 punct /,MCAD
R1958 T3065 T3061 punct −,MCAD
R1959 T3066 T3028 conj had,revealed
R1960 T3067 T3068 det a,elevation
R1961 T3068 T3075 nsubj elevation,evident
R1962 T3069 T3070 quantmod 5,6
R1963 T3070 T3073 quantmod 6,fold
R1964 T3071 T3070 punct -,6
R1965 T3072 T3070 quantmod to,6
R1966 T3073 T3068 nummod fold,elevation
R1967 T3074 T3073 punct -,fold
R1968 T3075 T3066 ccomp evident,had
R1969 T3076 T3068 prep of,elevation
R1970 T3077 T3078 compound serum,decenoylcarnitine
R1971 T3078 T3076 pobj decenoylcarnitine,of
R1972 T3079 T3075 prep in,evident
R1973 T3080 T3081 det the,profile
R1974 T3081 T3079 pobj profile,in
R1975 T3082 T3081 compound acylcarnitine,profile
R1976 T3083 T3084 punct (,Figure
R1977 T3084 T3066 parataxis Figure,had
R1978 T3085 T3084 nummod 4A,Figure
R1979 T3086 T3084 punct ),Figure
R1980 T3087 T3066 punct .,had
R1981 T3089 T3090 compound Bile,acylcarnitine
R1982 T3090 T3091 compound acylcarnitine,analysis
R1983 T3091 T3092 nsubj analysis,revealed
R1984 T3093 T3094 det a,pattern
R1985 T3094 T3092 dobj pattern,revealed
R1986 T3095 T3094 amod similar,pattern
R1987 T3096 T3094 compound acylcarnitine,pattern
R1988 T3097 T3094 prep as,pattern
R1989 T3098 T3097 prep in,as
R1990 T3099 T3098 pobj serum,in
R1991 T3100 T3101 punct (,Figure
R1992 T3101 T3092 parataxis Figure,revealed
R1993 T3102 T3101 nummod 4B,Figure
R1994 T3103 T3101 punct ),Figure
R1995 T3104 T3092 punct .,revealed
R1996 T3106 T3107 advmod However,are
R1997 T3108 T3107 punct ", ",are
R1998 T3109 T3110 det the,profiles
R1999 T3110 T3107 nsubj profiles,are
R2000 T3111 T3110 compound acylcarnitine,profiles
R2001 T3112 T3110 prep of,profiles
R2002 T3113 T3114 det the,mice
R2003 T3114 T3112 pobj mice,of
R2004 T3115 T3114 nmod MCAD,mice
R2005 T3116 T3115 punct −,MCAD
R2006 T3117 T3115 punct /,MCAD
R2007 T3118 T3115 punct −,MCAD
R2008 T3119 T3107 acomp different,are
R2009 T3120 T3119 prep from,different
R2010 T3121 T3120 pobj those,from
R2011 T3122 T3121 prep of,those
R2012 T3123 T3124 amod human,patients
R2013 T3124 T3122 pobj patients,of
R2014 T3125 T3126 npadvmod MCAD,deficient
R2015 T3126 T3124 amod deficient,patients
R2016 T3127 T3126 punct -,deficient
R2017 T3128 T3129 punct (,Figure
R2018 T3129 T3107 parataxis Figure,are
R2019 T3130 T3129 nummod 4C,Figure
R2020 T3131 T3129 punct ),Figure
R2021 T3132 T3107 punct .,are
R2022 T3134 T3135 amod Human,patients
R2023 T3135 T3136 nsubj patients,present
R2024 T3136 T3137 ccomp present,was
R2025 T3138 T3136 prep with,present
R2026 T3139 T3140 amod elevated,levels
R2027 T3140 T3138 pobj levels,with
R2028 T3141 T3140 prep of,levels
R2029 T3142 T3141 pobj C6,of
R2030 T3143 T3142 punct ", ",C6
R2031 T3144 T3142 conj C8,C6
R2032 T3145 T3144 punct ", ",C8
R2033 T3146 T3144 cc and,C8
R2034 T3147 T3144 conj C10,C8
R2035 T3148 T3147 punct :,C10
R2036 T3149 T3147 nummod 1,C10
R2037 T3150 T3142 appos acylcarnitines,C6
R2038 T3151 T3136 punct ", ",present
R2039 T3152 T3153 mark as,did
R2040 T3153 T3136 advcl did,present
R2041 T3154 T3155 det the,mice
R2042 T3155 T3153 nsubj mice,did
R2043 T3156 T3155 compound mutant,mice
R2044 T3157 T3137 punct ;,was
R2045 T3158 T3137 advmod however,was
R2046 T3159 T3137 punct ", ",was
R2047 T3160 T3161 det the,peak
R2048 T3161 T3137 nsubj peak,was
R2049 T3162 T3161 amod predominant,peak
R2050 T3163 T3164 compound C8,acylcarnitine
R2051 T3164 T3137 attr acylcarnitine,was
R2052 T3165 T3137 prep in,was
R2053 T3166 T3165 pobj humans,in
R2054 T3167 T3137 punct ", ",was
R2055 T3168 T3169 mark whereas,was
R2056 T3169 T3137 advcl was,was
R2057 T3170 T3169 prep in,was
R2058 T3171 T3172 det the,mouse
R2059 T3172 T3170 pobj mouse,in
R2060 T3173 T3169 nsubj it,was
R2061 T3174 T3175 nmod C10,acylcarnitine
R2062 T3175 T3169 attr acylcarnitine,was
R2063 T3176 T3174 punct :,C10
R2064 T3177 T3174 nummod 1,C10
R2065 T3178 T3137 punct .,was
R2070 T3319 T3320 amod Complete,examination
R2071 T3320 T3322 nsubj examination,demonstrated
R2072 T3321 T3320 amod histopathologic,examination
R2073 T3323 T3320 prep of,examination
R2074 T3324 T3325 nummod one,group
R2075 T3325 T3323 pobj group,of
R2076 T3326 T3325 prep of,group
R2077 T3327 T3328 nmod mutant,mice
R2078 T3328 T3326 pobj mice,of
R2079 T3329 T3327 cc and,mutant
R2080 T3330 T3327 conj MCAD,mutant
R2081 T3331 T3330 punct +,MCAD
R2082 T3332 T3330 punct /,MCAD
R2083 T3333 T3330 punct +,MCAD
R2084 T3334 T3328 compound control,mice
R2085 T3335 T3320 prep after,examination
R2086 T3336 T3337 det a,fast
R2087 T3337 T3335 pobj fast,after
R2088 T3338 T3339 nummod 24,h
R2089 T3339 T3337 compound h,fast
R2090 T3340 T3339 punct -,h
R2091 T3341 T3342 amod diffuse,steatosis
R2092 T3342 T3322 dobj steatosis,demonstrated
R2093 T3343 T3342 amod microvesicular,steatosis
R2094 T3344 T3343 cc and,microvesicular
R2095 T3345 T3343 conj macrovesicular,microvesicular
R2096 T3346 T3342 amod hepatic,steatosis
R2097 T3347 T3322 prep in,demonstrated
R2098 T3348 T3349 quantmod 6,8
R2099 T3349 T3351 nummod 8,wk
R2100 T3350 T3349 punct –,8
R2101 T3351 T3353 npadvmod wk,old
R2102 T3352 T3351 punct -,wk
R2103 T3353 T3355 amod old,mice
R2104 T3354 T3353 punct -,old
R2105 T3355 T3347 pobj mice,in
R2106 T3356 T3355 nmod MCAD,mice
R2107 T3357 T3356 punct −,MCAD
R2108 T3358 T3356 punct /,MCAD
R2109 T3359 T3356 punct −,MCAD
R2110 T3360 T3361 mark whereas,had
R2111 T3361 T3322 advcl had,demonstrated
R2112 T3362 T3363 nmod MCAD,mice
R2113 T3363 T3361 nsubj mice,had
R2114 T3364 T3362 punct +,MCAD
R2115 T3365 T3362 punct /,MCAD
R2116 T3366 T3362 punct +,MCAD
R2117 T3367 T3368 det no,changes
R2118 T3368 T3361 dobj changes,had
R2119 T3369 T3368 amod histologic,changes
R2120 T3370 T3371 punct (,5A
R2121 T3371 T3322 parataxis 5A,demonstrated
R2122 T3372 T3371 nmod Figure,5A
R2123 T3373 T3371 cc and,5A
R2124 T3374 T3371 conj 5B,5A
R2125 T3375 T3371 punct ),5A
R2126 T3376 T3322 punct .,demonstrated
R2127 T3378 T3379 prep In,were
R2128 T3380 T3381 det another,group
R2129 T3381 T3378 pobj group,In
R2130 T3382 T3381 prep of,group
R2131 T3383 T3384 nummod 4,wk
R2132 T3384 T3386 npadvmod wk,old
R2133 T3385 T3384 punct -,wk
R2134 T3386 T3388 amod old,mice
R2135 T3387 T3386 punct -,old
R2136 T3388 T3382 pobj mice,of
R2137 T3389 T3388 nmod MCAD,mice
R2138 T3390 T3389 punct +,MCAD
R2139 T3391 T3389 punct /,MCAD
R2140 T3392 T3389 punct +,MCAD
R2141 T3393 T3389 cc and,MCAD
R2142 T3394 T3389 conj MCAD,MCAD
R2143 T3395 T3394 punct −,MCAD
R2144 T3396 T3394 punct /,MCAD
R2145 T3397 T3394 punct −,MCAD
R2146 T3398 T3388 acl fasted,mice
R2147 T3399 T3398 prep for,fasted
R2148 T3400 T3401 nummod 24,h
R2187 T3443 T3442 punct −,MCAD
R2188 T3444 T3442 punct /,MCAD
R2189 T3445 T3442 punct −,MCAD
R2190 T3446 T3432 punct .,found
R2211 T3469 T3470 punct (,5C
R2212 T3470 T3449 parataxis 5C,had
R2213 T3471 T3470 nmod Figure,5C
R2214 T3472 T3470 cc and,5C
R2215 T3473 T3470 conj 5D,5C
R2216 T3474 T3470 punct ),5C
R2217 T3475 T3449 punct .,had
R2218 T3477 T3478 nsubj Changes,included
R2219 T3479 T3477 prep in,Changes
R2220 T3480 T3481 amod degenerating,myocytes
R2221 T3481 T3479 pobj myocytes,in
R2222 T3482 T3478 dobj swelling,included
R2223 T3483 T3482 punct ", ",swelling
R2224 T3484 T3485 amod pale,staining
R2225 T3485 T3482 conj staining,swelling
R2226 T3486 T3485 punct ", ",staining
R2227 T3487 T3485 cc and,staining
R2228 T3488 T3485 punct ", ",staining
R2229 T3489 T3490 prep in,replacement
R2230 T3490 T3485 conj replacement,staining
R2231 T3491 T3489 pobj portions,in
R2232 T3492 T3491 prep of,portions
R2233 T3493 T3494 det the,sarcoplasm
R2234 T3494 T3492 pobj sarcoplasm,of
R2235 T3495 T3490 punct ", ",replacement
R2236 T3496 T3490 prep of,replacement
R2237 T3497 T3496 pobj myofibrils,of
R2238 T3498 T3490 prep with,replacement
R2239 T3499 T3500 advmod finely,granular
R2240 T3500 T3501 amod granular,material
R2241 T3501 T3498 pobj material,with
R2242 T3502 T3501 amod eosinophilic,material
R2243 T3503 T3478 punct .,included
R2244 T3505 T3506 nsubj Nuclei,were
R2245 T3507 T3505 prep of,Nuclei
R2246 T3508 T3509 amod affected,myocytes
R2247 T3509 T3507 pobj myocytes,of
R2248 T3510 T3506 acomp large,were
R2249 T3511 T3510 punct ", ",large
R2250 T3512 T3510 conj pale,large
R2251 T3513 T3512 punct ", ",pale
R2252 T3514 T3512 cc and,pale
R2253 T3515 T3512 conj vesicular,pale
R2254 T3516 T3506 cc and,were
R2255 T3517 T3506 conj had,were
R2256 T3518 T3519 amod prominent,nucleoli
R2257 T3519 T3517 dobj nucleoli,had
R2258 T3520 T3506 punct .,were
R2259 T3522 T3523 prep In,was
R2260 T3524 T3525 det the,areas
R2261 T3525 T3522 pobj areas,In
R2262 T3526 T3527 advmod most,severely
R2263 T3527 T3528 advmod severely,affected
R2264 T3528 T3525 amod affected,areas
R2265 T3529 T3523 punct ", ",was
R2266 T3530 T3523 expl there,was
R2267 T3531 T3523 attr loss,was
R2268 T3532 T3531 prep of,loss
R2269 T3533 T3532 pobj myocytes,of
R2270 T3534 T3531 acl accompanied,loss
R2271 T3535 T3534 agent by,accompanied
R2272 T3536 T3535 pobj fibrosis,by
R2273 T3537 T3523 punct .,was
R2274 T3539 T3540 prep In,was
R2275 T3541 T3542 det the,wall
R2276 T3542 T3539 pobj wall,In
R2277 T3543 T3542 prep of,wall
R2278 T3544 T3545 det the,aorta
R2279 T3545 T3543 pobj aorta,of
R2280 T3546 T3545 prep at,aorta
R2281 T3547 T3548 det the,base
R2282 T3548 T3546 pobj base,at
R2283 T3549 T3548 prep of,base
R2284 T3550 T3551 det the,heart
R2285 T3551 T3549 pobj heart,of
R2286 T3552 T3540 expl there,was
R2287 T3553 T3554 amod multifocal,degeneration
R2288 T3554 T3540 attr degeneration,was
R2289 T3555 T3554 prep of,degeneration
R2290 T3556 T3557 det the,tissue
R2291 T3557 T3555 pobj tissue,of
R2292 T3558 T3557 amod elastic,tissue
R2293 T3559 T3554 punct ", ",degeneration
R2294 T3560 T3554 acl accompanied,degeneration
R2295 T3561 T3560 agent by,accompanied
R2296 T3562 T3563 amod multifocal,collections
R2297 T3563 T3561 pobj collections,by
R2298 T3564 T3563 prep of,collections
R2299 T3565 T3566 amod globular,pigment
R2300 T3566 T3564 pobj pigment,of
R2301 T3567 T3566 amod translucent,pigment
R2302 T3568 T3569 amod yellow,brown
R2303 T3569 T3566 amod brown,pigment
R2304 T3570 T3569 punct -,brown
R2305 T3571 T3563 acl interpreted,collections
R2306 T3572 T3573 aux to,be
R2307 T3573 T3571 xcomp be,interpreted
R2308 T3574 T3575 compound ceroid,lipofuscin
R2309 T3575 T3573 attr lipofuscin,be
R2310 T3576 T3575 punct /,lipofuscin
R2311 T3577 T3540 punct .,was
R2312 T3579 T3580 amod Similar,deposits
R2313 T3580 T3581 nsubjpass deposits,scattered
R2314 T3582 T3581 auxpass were,scattered
R2315 T3583 T3581 prep within,scattered
R2316 T3584 T3585 amod adjacent,tissue
R2317 T3585 T3583 pobj tissue,within
R2318 T3586 T3585 compound adipose,tissue
R2319 T3587 T3581 punct .,scattered
R2322 T3809 T3810 advmod Successfully,targeting
R2323 T3810 T3811 csubj targeting,produced
R2324 T3812 T3810 dobj Acadm,targeting
R2325 T3813 T3814 det a,model
R2326 T3814 T3811 dobj model,produced
R2327 T3815 T3814 compound mouse,model
R2328 T3816 T3814 prep for,model
R2329 T3817 T3818 compound MCAD,deficiency
R2330 T3818 T3816 pobj deficiency,for
R2331 T3819 T3814 prep with,model
R2332 T3820 T3819 pobj features,with
R2333 T3821 T3822 dep that,mimic
R2334 T3822 T3820 relcl mimic,features
R2335 T3823 T3824 det the,phenotype
R2336 T3824 T3822 dobj phenotype,mimic
R2337 T3825 T3824 amod clinical,phenotype
R2338 T3826 T3825 punct ", ",clinical
R2339 T3827 T3825 conj biochemical,clinical
R2340 T3828 T3827 punct ", ",biochemical
R2341 T3829 T3827 cc and,biochemical
R2342 T3830 T3827 conj pathologic,biochemical
R2343 T3831 T3824 acl found,phenotype
R2344 T3832 T3831 prep in,found
R2345 T3833 T3834 amod human,patients
R2346 T3834 T3832 pobj patients,in
R2347 T3835 T3811 punct .,produced
R2348 T3837 T3838 npadvmod MCAD,deficient
R2349 T3838 T3840 amod deficient,patients
R2350 T3839 T3838 punct -,deficient
R2351 T3840 T3841 nsubj patients,have
R2352 T3842 T3843 amod abnormal,metabolites
R2353 T3843 T3841 dobj metabolites,have
R2354 T3844 T3843 nmod plasma,metabolites
R2355 T3845 T3844 cc and,plasma
R2356 T3846 T3844 conj urine,plasma
R2357 T3847 T3843 acl associated,metabolites
R2358 T3848 T3847 prep with,associated
R2359 T3849 T3850 det the,specificity
R2360 T3850 T3848 pobj specificity,with
R2361 T3851 T3852 amod medium,chain
R2362 T3852 T3853 compound chain,length
R2363 T3853 T3850 compound length,specificity
R2364 T3854 T3853 punct –,length
R2365 T3855 T3850 compound enzyme,specificity
R2366 T3856 T3841 punct .,have
R2367 T3858 T3859 npadvmod MCAD,deficient
R2368 T3859 T3861 amod deficient,patients
R2369 T3860 T3859 punct -,deficient
R2370 T3861 T3862 nsubj patients,display
R2371 T3863 T3864 punct [,15
R2372 T3864 T3861 parataxis 15,patients
R2373 T3865 T3864 punct ],15
R2374 T3866 T3862 advmod often,display
R2375 T3867 T3868 det a,peak
R2376 T3868 T3862 dobj peak,display
R2377 T3869 T3868 amod characteristic,peak
R2378 T3870 T3868 amod urinary,peak
R2379 T3871 T3868 compound hexanoylglycine,peak
R2380 T3872 T3862 punct ", ",display
R2381 T3873 T3874 mark as,seen
R2382 T3874 T3862 advcl seen,display
R2383 T3875 T3874 auxpass was,seen
R2384 T3876 T3874 prep in,seen
R2385 T3877 T3878 nmod MCAD,mice
R2386 T3878 T3876 pobj mice,in
R2387 T3879 T3877 punct −,MCAD
R2388 T3880 T3877 punct /,MCAD
R2389 T3881 T3877 punct −,MCAD
R2390 T3882 T3862 punct .,display
R2391 T3884 T3885 compound Acylcarnitine,analysis
R2392 T3885 T3886 nsubj analysis,indicated
R2393 T3887 T3886 punct ", ",indicated
R2394 T3888 T3886 advmod however,indicated
R2395 T3889 T3886 punct ", ",indicated
R2396 T3890 T3891 compound mouse,MCAD
R2397 T3891 T3892 nsubj MCAD,is
R2398 T3892 T3886 advcl is,indicated
R2399 T3893 T3894 advmod more,active
R2400 T3894 T3892 acomp active,is
R2401 T3895 T3894 prep toward,active
R2402 T3896 T3897 amod longer,substrates
R2403 T3897 T3895 pobj substrates,toward
R2404 T3898 T3897 compound chain,substrates
R2405 T3899 T3894 prep than,active
R2406 T3900 T3901 det the,enzyme
R2407 T3901 T3899 pobj enzyme,than
R2408 T3902 T3901 amod human,enzyme
R2409 T3903 T3901 compound MCAD,enzyme
R2410 T3904 T3886 punct .,indicated
R2411 T3906 T3907 det This,finding
R2412 T3907 T3908 nsubj finding,is
R2413 T3909 T3908 acomp similar,is
R2414 T3910 T3909 prep to,similar
R2415 T3911 T3910 pobj that,to
R2416 T3912 T3911 acl seen,that
R2417 T3913 T3912 prep with,seen
R2418 T3914 T3915 advmod very,long
R2419 T3915 T3916 amod long,chain
R2420 T3916 T3918 compound chain,dehydrogenase
R2421 T3917 T3916 punct -,chain
R2422 T3918 T3913 pobj dehydrogenase,with
R2423 T3919 T3920 compound acyl,CoA
R2424 T3920 T3918 compound CoA,dehydrogenase
R2425 T3921 T3920 punct -,CoA
R2426 T3922 T3918 punct (,dehydrogenase
R2427 T3923 T3918 appos VLCAD,dehydrogenase
R2428 T3924 T3918 punct ),dehydrogenase
R2429 T3925 T3926 advmod where,is
R2430 T3926 T3918 relcl is,dehydrogenase
R2431 T3927 T3928 compound mouse,VLCAD
R2432 T3928 T3926 nsubj VLCAD,is
R2433 T3929 T3930 advmod most,active
R2434 T3930 T3926 acomp active,is
R2435 T3931 T3930 prep toward,active
R2436 T3932 T3933 compound C16,substrates
R2437 T3933 T3931 pobj substrates,toward
R2438 T3934 T3933 compound acyl,substrates
R2439 T3935 T3933 punct -,substrates
R2440 T3936 T3937 mark as,compared
R2441 T3937 T3926 advcl compared,is
R2442 T3938 T3937 prep to,compared
R2443 T3939 T3940 amod human,VLCAD
R2444 T3940 T3938 pobj VLCAD,to
R2445 T3941 T3940 prep with,VLCAD
R2446 T3942 T3943 det the,activity
R2447 T3943 T3941 pobj activity,with
R2448 T3944 T3943 advmod most,activity
R2449 T3945 T3943 amod enzymatic,activity
R2450 T3946 T3943 prep toward,activity
R2451 T3947 T3948 compound C14,substrates
R2452 T3948 T3946 pobj substrates,toward
R2453 T3949 T3948 compound acyl,substrates
R2454 T3950 T3948 punct -,substrates
R2455 T3951 T3952 punct [,16
R2456 T3952 T3908 parataxis 16,is
R2457 T3953 T3952 punct ],16
R2458 T3954 T3908 punct .,is
R2459 T3956 T3957 compound ETF,assays
R2460 T3957 T3959 nsubjpass assays,performed
R2461 T3958 T3957 compound reduction,assays
R2462 T3960 T3957 prep of,assays
R2463 T3961 T3962 compound liver,homogenates
R2464 T3962 T3960 pobj homogenates,of
R2465 T3963 T3959 auxpass were,performed
R2466 T3964 T3965 aux to,characterize
R2467 T3965 T3959 advcl characterize,performed
R2468 T3966 T3967 det the,mice
R2469 T3967 T3965 dobj mice,characterize
R2470 T3968 T3967 nmod MCAD,mice
R2471 T3969 T3968 punct −,MCAD
R2472 T3970 T3968 punct /,MCAD
R2473 T3971 T3968 punct −,MCAD
R2474 T3972 T3965 prep at,characterize
R2475 T3973 T3974 det the,level
R2476 T3974 T3972 pobj level,at
R2477 T3975 T3974 amod enzymatic,level
R2478 T3976 T3959 punct .,performed
R2479 T3978 T3979 nmod MCAD,mice
R2480 T3979 T3983 nsubj mice,had
R2481 T3980 T3978 punct −,MCAD
R2482 T3981 T3978 punct /,MCAD
R2483 T3982 T3978 punct −,MCAD
R2484 T3984 T3985 det a,ability
R2485 T3985 T3983 dobj ability,had
R2486 T3986 T3987 advmod significantly,reduced
R2487 T3987 T3985 amod reduced,ability
R2488 T3988 T3989 aux to,dehydrogenate
R2489 T3989 T3985 acl dehydrogenate,ability
R2490 T3990 T3991 compound C8,CoA
R2491 T3991 T3989 dobj CoA,dehydrogenate
R2492 T3992 T3991 punct -,CoA
R2493 T3993 T3983 punct ", ",had
R2494 T3994 T3995 mark as,is
R2495 T3995 T3983 advcl is,had
R2496 T3996 T3997 det the,case
R2497 T3997 T3995 attr case,is
R2498 T3998 T3995 prep in,is
R2499 T3999 T4000 amod human,patients
R2500 T4000 T3998 pobj patients,in
R2501 T4001 T4002 advmod where,reduced
R2502 T4002 T4000 relcl reduced,patients
R2503 T4003 T4004 compound MCAD,activity
R2504 T4004 T4002 nsubjpass activity,reduced
R2505 T4005 T4002 auxpass is,reduced
R2506 T4006 T4002 prep to,reduced
R2507 T4007 T4008 quantmod near,zero
R2508 T4008 T4006 pobj zero,to
R2509 T4009 T4002 prep with,reduced
R2510 T4010 T4011 compound C8,CoA
R2511 T4011 T4009 pobj CoA,with
R2512 T4012 T4011 punct -,CoA
R2513 T4013 T3983 punct .,had
R2514 T4015 T4016 nsubj This,was
R2515 T4017 T4018 advmod less,so
R2516 T4018 T4016 advmod so,was
R2517 T4019 T4016 prep with,was
R2518 T4020 T4021 compound palmitoyl,CoA
R2519 T4021 T4019 pobj CoA,with
R2520 T4022 T4021 punct -,CoA
R2521 T4023 T4024 punct (,C16
R2522 T4024 T4016 parataxis C16,was
R2523 T4025 T4024 punct :,C16
R2524 T4026 T4024 nummod 0,C16
R2525 T4027 T4024 punct ),C16
R2526 T4028 T4016 punct .,was
R2527 T4030 T4031 mark Because,was
R2528 T4031 T4033 advcl was,represent
R2529 T4032 T4031 expl there,was
R2530 T4034 T4031 advmod clearly,was
R2531 T4035 T4036 det no,antigen
R2532 T4036 T4031 attr antigen,was
R2533 T4037 T4036 compound MCAD,antigen
R2534 T4038 T4036 acl detected,antigen
R2535 T4039 T4038 prep in,detected
R2536 T4040 T4041 nmod MCAD,mice
R2537 T4041 T4039 pobj mice,in
R2538 T4042 T4040 punct −,MCAD
R2539 T4043 T4040 punct /,MCAD
R2540 T4044 T4040 punct −,MCAD
R2541 T4045 T4033 punct ", ",represent
R2542 T4046 T4047 det the,activity
R2543 T4047 T4033 nsubj activity,represent
R2544 T4048 T4047 amod residual,activity
R2545 T4049 T4047 compound dehydrogenase,activity
R2546 T4050 T4047 acl measured,activity
R2547 T4051 T4050 prep with,measured
R2548 T4052 T4053 det these,substrates
R2549 T4053 T4051 pobj substrates,with
R2550 T4054 T4053 nummod two,substrates
R2551 T4055 T4033 aux must,represent
R2552 T4056 T4057 det the,activity
R2553 T4057 T4033 dobj activity,represent
R2554 T4058 T4057 prep of,activity
R2555 T4059 T4060 amod other,dehydrogenases
R2556 T4060 T4058 pobj dehydrogenases,of
R2557 T4061 T4062 compound chain,length
R2558 T4062 T4063 npadvmod length,specific
R2559 T4063 T4060 amod specific,dehydrogenases
R2560 T4064 T4063 punct –,specific
R2561 T4065 T4066 compound acyl,CoA
R2562 T4066 T4060 compound CoA,dehydrogenases
R2563 T4067 T4066 punct -,CoA
R2564 T4068 T4033 punct .,represent
R2565 T4070 T4071 det A,degree
R2566 T4071 T4073 nsubj degree,was
R2567 T4072 T4071 amod high,degree
R2568 T4074 T4071 prep of,degree
R2569 T4075 T4076 amod neonatal,mortality
R2570 T4076 T4074 pobj mortality,of
R2571 T4077 T4071 prep in,degree
R2572 T4078 T4079 nmod MCAD,mice
R2573 T4079 T4077 pobj mice,in
R2574 T4080 T4078 punct −,MCAD
R2575 T4081 T4078 punct /,MCAD
R2576 T4082 T4078 punct −,MCAD
R2577 T4083 T4084 det a,observation
R2578 T4084 T4073 attr observation,was
R2579 T4085 T4084 amod striking,observation
R2580 T4086 T4073 cc and,was
R2581 T4087 T4073 conj appears,was
R2582 T4088 T4089 aux to,be
R2583 T4089 T4087 xcomp be,appears
R2584 T4090 T4089 acomp analogous,be
R2585 T4091 T4090 prep to,analogous
R2586 T4092 T4093 det the,patterns
R2587 T4093 T4091 pobj patterns,to
R2588 T4094 T4093 prep of,patterns
R2589 T4095 T4096 amod clinical,episodes
R2590 T4096 T4094 pobj episodes,of
R2591 T4097 T4096 cc and,episodes
R2592 T4098 T4096 conj mortality,episodes
R2593 T4099 T4093 prep in,patterns
R2594 T4100 T4101 npadvmod MCAD,deficient
R2595 T4101 T4103 amod deficient,patients
R2596 T4102 T4101 punct -,deficient
R2597 T4103 T4099 pobj patients,in
R2598 T4104 T4073 punct .,was
R2599 T4106 T4107 nmod MCAD,mice
R2600 T4107 T4111 nsubj mice,exhibited
R2601 T4108 T4106 punct −,MCAD
R2602 T4109 T4106 punct /,MCAD
R2603 T4110 T4106 punct −,MCAD
R2604 T4112 T4113 amod significant,mortality
R2605 T4113 T4111 dobj mortality,exhibited
R2606 T4114 T4113 amod neonatal,mortality
R2607 T4115 T4111 prep with,exhibited
R2608 T4116 T4117 advmod approximately,60
R2609 T4117 T4118 nummod 60,%
R2610 T4118 T4119 nsubj %,dying
R2611 T4119 T4115 pcomp dying,with
R2612 T4120 T4118 prep of,%
R2613 T4121 T4122 det the,pups
R2614 T4122 T4120 pobj pups,of
R2615 T4123 T4122 nmod MCAD,pups
R2616 T4124 T4123 punct −,MCAD
R2617 T4125 T4123 punct /,MCAD
R2618 T4126 T4123 punct −,MCAD
R2619 T4127 T4128 amod prior,to
R2620 T4128 T4119 prep to,dying
R2621 T4129 T4128 pobj weaning,to
R2622 T4130 T4119 prep at,dying
R2623 T4131 T4132 nummod 3,wk
R2624 T4132 T4130 pobj wk,at
R2625 T4133 T4132 prep of,wk
R2626 T4134 T4133 pobj age,of
R2627 T4135 T4111 punct .,exhibited
R2628 T4137 T4138 amod Human,patients
R2629 T4138 T4139 nsubj patients,present
R2630 T4140 T4139 prep with,present
R2631 T4141 T4140 pobj hypoglycemia,with
R2632 T4142 T4141 punct ", ",hypoglycemia
R2633 T4143 T4141 conj hypoketonemia,hypoglycemia
R2634 T4144 T4143 punct ", ",hypoketonemia
R2635 T4145 T4143 cc and,hypoketonemia
R2636 T4146 T4147 amod nonketotic,aciduria
R2637 T4147 T4143 conj aciduria,hypoketonemia
R2638 T4148 T4147 amod organic,aciduria
R2639 T4149 T4147 acl precipitated,aciduria
R2640 T4150 T4149 agent by,precipitated
R2641 T4151 T4150 pobj fasting,by
R2642 T4152 T4139 punct ", ",present
R2643 T4153 T4154 advmod most,frequently
R2644 T4154 T4155 advmod frequently,during
R2645 T4155 T4139 prep during,present
R2646 T4156 T4157 det the,mo
R2647 T4157 T4155 pobj mo,during
R2648 T4158 T4157 amod first,mo
R2649 T4159 T4157 nummod 24,mo
R2650 T4160 T4157 prep in,mo
R2651 T4161 T4160 pobj life,in
R2652 T4162 T4163 punct [,1
R2653 T4163 T4139 parataxis 1,present
R2654 T4164 T4163 punct ],1
R2655 T4165 T4139 punct .,present
R2656 T4167 T4168 nsubj It,is
R2657 T4169 T4168 acomp likely,is
R2658 T4170 T4171 mark that,manifesting
R2659 T4171 T4168 ccomp manifesting,is
R2660 T4172 T4173 amod neonatal,pups
R2661 T4173 T4171 nsubj pups,manifesting
R2662 T4174 T4173 nmod MCAD,pups
R2663 T4175 T4174 punct −,MCAD
R2664 T4176 T4174 punct /,MCAD
R2665 T4177 T4174 punct −,MCAD
R2666 T4178 T4171 aux are,manifesting
R2667 T4179 T4171 dobj sensitivity,manifesting
R2668 T4180 T4179 prep to,sensitivity
R2669 T4181 T4180 pobj fasting,to
R2670 T4182 T4171 prep with,manifesting
R2671 T4183 T4182 pobj decompensation,with
R2672 T4184 T4183 prep in,decompensation
R2673 T4185 T4186 det a,period
R2674 T4186 T4184 pobj period,in
R2675 T4187 T4186 amod short,period
R2676 T4188 T4186 prep of,period
R2677 T4189 T4188 pobj time,of
R2678 T4190 T4191 mark if,ingested
R2679 T4191 T4171 advcl ingested,manifesting
R2680 T4192 T4193 amod maternal,milk
R2681 T4193 T4191 nsubjpass milk,ingested
R2682 T4194 T4191 auxpass is,ingested
R2683 T4195 T4191 neg not,ingested
R2684 T4196 T4168 punct .,is
R2685 T4198 T4199 prep In,noted
R2686 T4200 T4198 pobj contrast,In
R2687 T4201 T4199 punct ", ",noted
R2688 T4202 T4203 det no,mortality
R2689 T4203 T4199 nsubjpass mortality,noted
R2690 T4204 T4199 auxpass was,noted
R2691 T4205 T4199 prep in,noted
R2692 T4206 T4207 amod adult,mice
R2693 T4207 T4205 pobj mice,in
R2694 T4208 T4207 nmod MCAD,mice
R2695 T4209 T4208 punct −,MCAD
R2696 T4210 T4208 punct /,MCAD
R2697 T4211 T4208 punct −,MCAD
R2698 T4212 T4213 mark unless,challenged
R2699 T4213 T4199 advcl challenged,noted
R2700 T4214 T4213 prep with,challenged
R2701 T4215 T4216 compound cold,stress
R2702 T4216 T4214 pobj stress,with
R2703 T4217 T4216 cc and,stress
R2704 T4218 T4216 conj fasting,stress
R2705 T4219 T4199 punct .,noted
R2706 T4221 T4222 prep Under,were
R2707 T4223 T4224 compound cold,challenge
R2708 T4224 T4225 compound challenge,conditions
R2709 T4225 T4221 pobj conditions,Under
R2710 T4226 T4222 punct ", ",were
R2711 T4227 T4222 advmod however,were
R2712 T4228 T4222 punct ", ",were
R2713 T4229 T4230 nmod MCAD,mice
R2714 T4230 T4222 nsubj mice,were
R2715 T4231 T4229 punct −,MCAD
R2716 T4232 T4229 punct /,MCAD
R2717 T4233 T4229 punct −,MCAD
R2718 T4234 T4222 acomp unable,were
R2719 T4235 T4236 aux to,maintain
R2720 T4236 T4234 xcomp maintain,unable
R2721 T4237 T4238 compound body,temperature
R2722 T4238 T4236 dobj temperature,maintain
R2723 T4239 T4222 punct .,were
R2724 T4241 T4242 amod Brown,fat
R2725 T4242 T4243 nsubj fat,is
R2726 T4244 T4245 advmod predominantly,responsible
R2727 T4245 T4243 acomp responsible,is
R2728 T4246 T4245 prep for,responsible
R2729 T4247 T4246 pobj thermogenesis,for
R2730 T4248 T4243 cc and,is
R2731 T4249 T4250 advmod normally,expresses
R2732 T4250 T4243 conj expresses,is
R2733 T4251 T4252 amod high,levels
R2734 T4252 T4250 dobj levels,expresses
R2735 T4253 T4252 prep of,levels
R2736 T4254 T4255 compound Acadm,mRNA
R2737 T4255 T4253 pobj mRNA,of
R2738 T4256 T4243 punct .,is
R2739 T4258 T4259 det The,steatosis
R2740 T4259 T4264 nsubj steatosis,is
R2741 T4260 T4259 amod microvesicular,steatosis
R2742 T4261 T4260 cc and,microvesicular
R2743 T4262 T4260 conj macrovesicular,microvesicular
R2744 T4263 T4259 amod hepatic,steatosis
R2745 T4265 T4259 acl seen,steatosis
R2746 T4266 T4265 prep in,seen
R2747 T4267 T4268 amod fasted,mice
R2748 T4268 T4266 pobj mice,in
R2749 T4269 T4268 nmod MCAD,mice
R2750 T4270 T4269 punct −,MCAD
R2751 T4271 T4269 punct /,MCAD
R2752 T4272 T4269 punct −,MCAD
R2753 T4273 T4264 acomp consistent,is
R2754 T4274 T4273 prep with,consistent
R2755 T4275 T4276 det the,finding
R2756 T4276 T4274 pobj finding,with
R2757 T4277 T4276 amod primary,finding
R2758 T4278 T4276 amod pathological,finding
R2759 T4279 T4276 acl seen,finding
R2760 T4280 T4279 prep in,seen
R2761 T4281 T4282 amod human,patients
R2762 T4282 T4280 pobj patients,in
R2763 T4283 T4282 compound MCAD,patients
R2764 T4284 T4282 prep with,patients
R2765 T4285 T4286 compound fasting,stress
R2766 T4286 T4284 pobj stress,with
R2767 T4287 T4264 punct .,is
R2768 T4289 T4290 amod Sporadic,lesions
R2769 T4290 T4292 nsubj lesions,were
R2770 T4291 T4290 amod cardiac,lesions
R2771 T4293 T4290 prep in,lesions
R2772 T4294 T4295 nmod MCAD,mice
R2773 T4295 T4293 pobj mice,in
R2774 T4296 T4294 punct −,MCAD
R2775 T4297 T4294 punct /,MCAD
R2776 T4298 T4294 punct −,MCAD
R2777 T4299 T4292 punct ", ",were
R2778 T4300 T4292 advmod however,were
R2779 T4301 T4292 punct ", ",were
R2780 T4302 T4303 det an,finding
R2781 T4303 T4292 attr finding,were
R2782 T4304 T4303 amod interesting,finding
R2783 T4305 T4304 cc and,interesting
R2784 T4306 T4304 conj unexpected,interesting
R2785 T4307 T4292 punct .,were
R2786 T4309 T4310 det The,cardiomyopathy
R2787 T4310 T4312 nsubjpass cardiomyopathy,reported
R2788 T4311 T4310 amod diffuse,cardiomyopathy
R2789 T4312 T4329 ccomp reported,reported
R2790 T4313 T4310 prep with,cardiomyopathy
R2791 T4314 T4315 amod multifocal,degeneration
R2792 T4315 T4313 pobj degeneration,with
R2793 T4316 T4315 compound myocyte,degeneration
R2794 T4317 T4315 cc and,degeneration
R2795 T4318 T4315 conj necrosis,degeneration
R2796 T4319 T4310 acl observed,cardiomyopathy
R2797 T4320 T4319 prep in,observed
R2798 T4321 T4322 nmod MCAD,mice
R2799 T4322 T4320 pobj mice,in
R2800 T4323 T4321 punct −,MCAD
R2801 T4324 T4321 punct /,MCAD
R2802 T4325 T4321 punct −,MCAD
R2803 T4326 T4312 aux has,reported
R2804 T4327 T4312 neg not,reported
R2805 T4328 T4312 auxpass been,reported
R2806 T4330 T4312 prep in,reported
R2807 T4331 T4332 amod human,patients
R2808 T4332 T4330 pobj patients,in
R2809 T4333 T4332 compound MCAD,patients
R2810 T4334 T4329 punct ", ",reported
R2811 T4335 T4329 advmod however,reported
R2812 T4336 T4329 punct ", ",reported
R2813 T4337 T4338 amod cardiac,arrhythmias
R2814 T4338 T4329 nsubjpass arrhythmias,reported
R2815 T4339 T4338 cc and,arrhythmias
R2816 T4340 T4338 conj dysfunction,arrhythmias
R2817 T4341 T4329 aux have,reported
R2818 T4342 T4329 auxpass been,reported
R2819 T4343 T4329 prep in,reported
R2820 T4344 T4345 npadvmod MCAD,deficient
R2821 T4345 T4347 amod deficient,patients
R2822 T4346 T4345 punct -,deficient
R2823 T4347 T4343 pobj patients,in
R2824 T4348 T4349 punct [,18
R2825 T4349 T4329 parataxis 18,reported
R2826 T4350 T4349 nummod 17,18
R2827 T4351 T4349 punct ", ",18
R2828 T4352 T4349 punct ],18
R2829 T4353 T4329 punct .,reported
R2830 T4355 T4356 advmod Interestingly,observed
R2831 T4357 T4356 punct ", ",observed
R2832 T4358 T4356 nsubjpass cardiomyopathy,observed
R2833 T4359 T4356 aux has,observed
R2834 T4360 T4356 auxpass been,observed
R2835 T4361 T4356 prep in,observed
R2836 T4362 T4363 compound VLCAD,deficiency
R2837 T4363 T4361 pobj deficiency,in
R2838 T4364 T4365 punct [,19
R2839 T4365 T4363 parataxis 19,deficiency
R2840 T4366 T4365 punct ],19
R2841 T4367 T4363 cc and,deficiency
R2842 T4368 T4369 amod other,disorders
R2843 T4369 T4363 conj disorders,deficiency
R2844 T4370 T4369 prep of,disorders
R2845 T4371 T4372 amod long,chain
R2846 T4372 T4373 compound chain,fat
R2847 T4373 T4374 compound fat,metabolism
R2848 T4374 T4370 pobj metabolism,of
R2849 T4375 T4376 amod such,as
R2850 T4376 T4369 prep as,disorders
R2851 T4377 T4378 amod severe,deficiencies
R2852 T4378 T4376 pobj deficiencies,as
R2853 T4379 T4380 nmod CPT,1
R2854 T4380 T4378 nummod 1,deficiencies
R2855 T4381 T4380 punct -,1
R2856 T4382 T4380 cc and,1
R2857 T4383 T4384 punct -,2
R2858 T4384 T4380 conj 2,1
R2859 T4385 T4386 punct [,1
R2860 T4386 T4356 parataxis 1,observed
R2861 T4387 T4386 punct ],1
R2862 T4388 T4356 punct .,observed
R2863 T4390 T4391 advmod Thus,is
R2864 T4392 T4391 nsubj it,is
R2865 T4393 T4391 acomp tempting,is
R2866 T4394 T4395 aux to,relate
R2867 T4395 T4391 xcomp relate,is
R2868 T4396 T4397 det the,problems
R2869 T4397 T4395 dobj problems,relate
R2870 T4398 T4397 amod cardiac,problems
R2871 T4399 T4397 prep in,problems
R2872 T4400 T4401 det the,mouse
R2873 T4401 T4399 pobj mouse,in
R2874 T4402 T4395 prep to,relate
R2875 T4403 T4404 det the,range
R2876 T4404 T4402 pobj range,to
R2877 T4405 T4404 amod apparent,range
R2878 T4406 T4404 amod broader,range
R2879 T4407 T4404 prep of,range
R2880 T4408 T4409 compound substrate,utilization
R2881 T4409 T4407 pobj utilization,of
R2882 T4410 T4409 prep of,utilization
R2883 T4411 T4412 compound mouse,MCAD
R2884 T4412 T4410 pobj MCAD,of
R2885 T4413 T4391 punct .,is
R2886 T4415 T4416 det The,lesions
R2887 T4416 T4421 nsubj lesions,is
R2888 T4417 T4416 amod inconsistent,lesions
R2889 T4418 T4416 nmod liver,lesions
R2890 T4419 T4418 cc and,liver
R2891 T4420 T4418 conj cardiac,liver
R2892 T4422 T4416 prep in,lesions
R2893 T4423 T4424 det these,mice
R2894 T4424 T4422 pobj mice,in
R2895 T4425 T4421 acomp analogous,is
R2896 T4426 T4425 prep with,analogous
R2897 T4427 T4428 det the,heterogeneity
R2898 T4428 T4426 pobj heterogeneity,with
R2899 T4429 T4428 amod significant,heterogeneity
R2900 T4430 T4431 advmod inter,intrafamilial
R2901 T4431 T4428 amod intrafamilial,heterogeneity
R2902 T4432 T4431 punct -,intrafamilial
R2903 T4433 T4431 cc and,intrafamilial
R2904 T4434 T4428 amod phenotypic,heterogeneity
R2905 T4435 T4428 acl seen,heterogeneity
R2906 T4436 T4435 prep in,seen
R2907 T4437 T4438 compound MCAD,deficiency
R2908 T4438 T4436 pobj deficiency,in
R2909 T4439 T4435 prep in,seen
R2910 T4440 T4439 pobj humans,in
R2911 T4441 T4442 punct [,20
R2912 T4442 T4421 parataxis 20,is
R2913 T4443 T4442 nummod 1,20
R2914 T4444 T4442 punct ",",20
R2915 T4445 T4442 punct ],20
R2916 T4446 T4421 punct .,is
R2917 T4448 T4449 prep In,is
R2918 T4450 T4448 pobj comparisons,In
R2919 T4451 T4450 prep with,comparisons
R2920 T4452 T4453 poss our,experiences
R2921 T4453 T4451 pobj experiences,with
R2922 T4454 T4453 prep with,experiences
R2923 T4455 T4456 det the,models
R2924 T4456 T4454 pobj models,with
R2925 T4457 T4456 amod other,models
R2926 T4458 T4456 compound mouse,models
R2927 T4459 T4456 prep for,models
R2928 T4460 T4461 compound acyl,CoA
R2929 T4461 T4463 compound CoA,dehydrogenase
R2930 T4462 T4461 punct -,CoA
R2931 T4463 T4464 compound dehydrogenase,deficiencies
R2932 T4464 T4459 pobj deficiencies,for
R2933 T4465 T4449 punct ", ",is
R2934 T4466 T4467 det the,phenotype
R2935 T4467 T4449 nsubj phenotype,is
R2936 T4468 T4467 amod overall,phenotype
R2937 T4469 T4467 prep of,phenotype
R2938 T4470 T4471 nmod MCAD,mice
R2939 T4471 T4469 pobj mice,of
R2940 T4472 T4470 punct −,MCAD
R2941 T4473 T4470 punct /,MCAD
R2942 T4474 T4470 punct −,MCAD
R2943 T4475 T4476 advmod less,severe
R2944 T4476 T4449 acomp severe,is
R2945 T4477 T4476 prep than,severe
R2946 T4478 T4477 pobj that,than
R2947 T4479 T4478 acl found,that
R2948 T4480 T4479 prep in,found
R2949 T4481 T4482 nmod LCAD,mice
R2950 T4482 T4480 pobj mice,in
R2951 T4483 T4481 punct −,LCAD
R2952 T4484 T4481 punct /,LCAD
R2953 T4485 T4481 punct −,LCAD
R2954 T4486 T4476 punct ", ",severe
R2955 T4487 T4476 cc yet,severe
R2956 T4488 T4489 advmod more,pronounced
R2957 T4489 T4476 conj pronounced,severe
R2958 T4490 T4489 prep than,pronounced
R2959 T4491 T4492 det the,models
R2960 T4492 T4490 pobj models,than
R2961 T4493 T4492 nmod VLCAD,models
R2962 T4494 T4493 punct −,VLCAD
R2963 T4495 T4493 punct /,VLCAD
R2964 T4496 T4493 punct −,VLCAD
R2965 T4497 T4493 cc or,VLCAD
R2966 T4498 T4493 conj SCAD,VLCAD
R2967 T4499 T4498 punct −,SCAD
R2968 T4500 T4498 punct /,SCAD
R2969 T4501 T4498 punct −,SCAD
R2970 T4502 T4492 compound mouse,models
R2971 T4503 T4504 punct [,22
R2972 T4504 T4449 parataxis 22,is
R2973 T4505 T4504 nummod 16,22
R2974 T4506 T4504 punct ",",22
R2975 T4507 T4504 nummod 21,22
R2976 T4508 T4504 punct ",",22
R2977 T4509 T4504 punct ],22
R2978 T4510 T4449 punct .,is
R2979 T4512 T4513 nsubj All,are
R2980 T4514 T4512 prep of,All
R2981 T4515 T4516 det these,mutants
R2982 T4516 T4514 pobj mutants,of
R2983 T4517 T4518 npadvmod cold,intolerant
R2984 T4518 T4513 acomp intolerant,are
R2985 T4519 T4513 cc and,are
R2986 T4520 T4513 conj display,are
R2987 T4521 T4522 amod varying,degrees
R2988 T4522 T4520 dobj degrees,display
R2989 T4523 T4522 prep of,degrees
R2990 T4524 T4525 amod fatty,changes
R2991 T4525 T4523 pobj changes,of
R2992 T4526 T4520 prep in,display
R2993 T4527 T4526 pobj liver,in
R2994 T4528 T4527 punct ", ",liver
R2995 T4529 T4527 conj heart,liver
R2996 T4530 T4529 punct ", ",heart
R2997 T4531 T4529 cc and,heart
R2998 T4532 T4529 conj kidney,heart
R2999 T4533 T4513 punct .,are
R3000 T4535 T4536 nmod LCAD,mice
R3001 T4536 T4540 nsubj mice,show
R3002 T4537 T4535 punct −,LCAD
R3003 T4538 T4535 punct /,LCAD
R3004 T4539 T4535 punct −,LCAD
R3005 T4541 T4542 amod more,deaths
R3006 T4542 T4540 dobj deaths,show
R3007 T4543 T4542 amod spontaneous,deaths
R3008 T4544 T4542 cc and,deaths
R3009 T4545 T4546 amod gestational,losses
R3010 T4546 T4542 conj losses,deaths
R3011 T4547 T4542 prep than,deaths
R3012 T4548 T4549 det the,deficiencies
R3013 T4549 T4547 pobj deficiencies,than
R3014 T4550 T4549 amod other,deficiencies
R3015 T4551 T4552 punct [,21
R3016 T4552 T4540 parataxis 21,show
R3017 T4553 T4552 punct ],21
R3018 T4554 T4540 punct .,show
R3019 T4556 T4557 det The,mortality
R3020 T4557 T4560 nsubj mortality,is
R3021 T4558 T4557 amod significant,mortality
R3022 T4559 T4557 amod neonatal,mortality
R3023 T4561 T4557 prep in,mortality
R3024 T4562 T4563 nmod MCAD,mice
R3025 T4563 T4561 pobj mice,in
R3026 T4564 T4562 punct −,MCAD
R3027 T4565 T4562 punct /,MCAD
R3028 T4566 T4562 punct −,MCAD
R3029 T4567 T4560 acomp distinctive,is
R3030 T4568 T4567 prep from,distinctive
R3031 T4569 T4570 det these,models
R3032 T4570 T4568 pobj models,from
R3033 T4571 T4570 amod other,models
R3034 T4572 T4570 compound mouse,models
R3040 T4578 T4577 pobj sensitivity,of
R3041 T4579 T4578 prep to,sensitivity
R3042 T4580 T4581 compound fasting,intolerance
R3043 T4581 T4579 pobj intolerance,to
R3044 T4582 T4560 punct .,is
R3045 T4584 T4585 det The,phenotypes
R3046 T4585 T4586 nsubj phenotypes,are
R3047 T4587 T4585 prep of,phenotypes
R3048 T4588 T4589 preconj both,mice
R3049 T4589 T4587 pobj mice,of
R3050 T4590 T4589 det the,mice
R3051 T4591 T4589 nmod VLCAD,mice
R3052 T4592 T4591 punct −,VLCAD
R3053 T4593 T4591 punct /,VLCAD
R3054 T4594 T4591 punct −,VLCAD
R3055 T4595 T4591 cc and,VLCAD
R3056 T4596 T4591 conj SCAD,VLCAD
R3057 T4597 T4596 punct −,SCAD
R3058 T4598 T4596 punct /,SCAD
R3059 T4599 T4596 punct −,SCAD
R3060 T4600 T4601 advmod relatively,mild
R3061 T4601 T4586 acomp mild,are
R3062 T4602 T4603 mark if,stressed
R3063 T4603 T4586 advcl stressed,are
R3064 T4604 T4605 det the,animals
R3065 T4605 T4603 nsubjpass animals,stressed
R3066 T4606 T4603 auxpass are,stressed
R3067 T4607 T4603 neg not,stressed
R3068 T4608 T4603 amod cold,stressed
R3069 T4609 T4610 punct [,22
R3070 T4610 T4586 parataxis 22,are
R3071 T4611 T4610 nummod 16,22
R3072 T4612 T4610 punct ",",22
R3073 T4613 T4610 punct ],22
R3074 T4614 T4586 punct .,are
R3075 T4616 T4617 det The,mouse
R3076 T4617 T4621 nsubj mouse,offers
R3077 T4618 T4619 amod MCAD,deficient
R3078 T4619 T4617 amod deficient,mouse
R3079 T4620 T4619 punct -,deficient
R3080 T4622 T4623 amod new,insights
R3081 T4623 T4621 dobj insights,offers
R3082 T4624 T4623 prep into,insights
R3083 T4625 T4626 det the,pathogenesis
R3084 T4626 T4624 pobj pathogenesis,into
R3085 T4627 T4626 prep of,pathogenesis
R3086 T4628 T4629 amod mitochondrial,deficiencies
R3087 T4629 T4627 pobj deficiencies,of
R3088 T4630 T4631 compound β,oxidation
R3089 T4631 T4629 compound oxidation,deficiencies
R3090 T4632 T4631 punct -,oxidation
R3091 T4633 T4621 cc and,offers
R3092 T4634 T4635 aux will,provide
R3093 T4635 T4621 conj provide,offers
R3094 T4636 T4637 det a,tool
R3095 T4637 T4635 dobj tool,provide
R3096 T4638 T4637 amod robust,tool
R3097 T4639 T4640 aux to,understand
R3098 T4640 T4637 advcl understand,tool
R3099 T4641 T4640 advmod better,understand
R3100 T4642 T4643 det the,role
R3101 T4643 T4640 dobj role,understand
R3102 T4644 T4643 prep of,role
R3103 T4645 T4646 amod fatty,acids
R3104 T4646 T4644 pobj acids,of
R3105 T4647 T4643 prep in,role
R3106 T4648 T4649 amod other,diseases
R3107 T4649 T4647 pobj diseases,in
R3108 T4650 T4649 amod relevant,diseases
R3109 T4651 T4621 punct .,offers
R3112 T4938 T4937 prep of,Construction
R3113 T4939 T4940 compound MCAD,vector
R3114 T4940 T4938 pobj vector,of
R3115 T4941 T4940 compound targeting,vector
R3116 T4942 T4937 punct .,Construction
R3117 T4944 T4945 det A,cassette
R3118 T4945 T4949 nsubjpass cassette,subcloned
R3119 T4946 T4947 compound neomycin,resistance
R3120 T4947 T4945 compound resistance,cassette
R3121 T4948 T4945 compound gene,cassette
R3122 T4950 T4951 punct [,23
R3123 T4951 T4945 parataxis 23,cassette
R3124 T4952 T4951 punct ],23
R3125 T4953 T4949 auxpass was,subcloned
R3126 T4954 T4949 prep into,subcloned
R3127 T4955 T4956 det the,site
R3128 T4956 T4954 pobj site,into
R3129 T4957 T4956 compound SalI,site
R3130 T4958 T4956 prep of,site
R3131 T4959 T4958 pobj pGEM11Zf,of
R3132 T4960 T4961 punct (,+
R3133 T4961 T4959 punct +,pGEM11Zf
R3134 T4962 T4961 punct ),+
R3135 T4963 T4949 punct .,subcloned
R3136 T4965 T4966 det The,plasmid
R3137 T4966 T4967 nsubjpass plasmid,digested
R3138 T4968 T4967 auxpass was,digested
R3139 T4969 T4967 prep with,digested
R3140 T4970 T4969 pobj EcoRI,with
R3141 T4971 T4967 cc and,digested
R3142 T4972 T4973 det the,overhangs
R3143 T4973 T4974 nsubjpass overhangs,filled
R3144 T4974 T4967 conj filled,digested
R3145 T4975 T4974 auxpass were,filled
R3146 T4976 T4974 prep with,filled
R3147 T4977 T4978 compound Klenow,enzyme
R3148 T4978 T4976 pobj enzyme,with
R3149 T4979 T4974 punct .,filled
R3150 T4981 T4982 amod Subsequent,ligation
R3151 T4982 T4983 nsubj ligation,recircularized
R3152 T4984 T4982 prep of,ligation
R3153 T4985 T4986 det the,ends
R3154 T4986 T4984 pobj ends,of
R3155 T4987 T4986 compound blunt,ends
R3156 T4988 T4989 det the,vector
R3157 T4989 T4983 dobj vector,recircularized
R3158 T4990 T4983 cc and,recircularized
R3159 T4991 T4983 conj destroyed,recircularized
R3160 T4992 T4993 det the,site
R3161 T4993 T4991 dobj site,destroyed
R3162 T4994 T4993 compound EcoRI,site
R3163 T4995 T4993 prep within,site
R3164 T4996 T4997 det the,polylinker
R3165 T4997 T4995 pobj polylinker,within
R3166 T4998 T4997 prep of,polylinker
R3167 T4999 T5000 det the,plasmid
R3168 T5000 T4998 pobj plasmid,of
R3169 T5001 T5000 nmod pGEMl1Zf,plasmid
R3170 T5002 T5003 punct (,+
R3171 T5003 T5000 punct +,plasmid
R3172 T5004 T5003 punct ),+
R3173 T5005 T4983 punct .,recircularized
R3174 T5007 T5008 advmod Next,cloned
R3175 T5009 T5008 punct ", ",cloned
R3176 T5010 T5011 det an,fragment
R3177 T5011 T5008 nsubjpass fragment,cloned
R3178 T5012 T5013 nummod 8,kb
R3179 T5013 T5011 nmod kb,fragment
R3180 T5014 T5013 punct -,kb
R3181 T5015 T5011 nmod Acadm,fragment
R3182 T5016 T5011 amod genomic,fragment
R3183 T5017 T5011 acl spanning,fragment
R3184 T5018 T5019 nmod exons,8
R3185 T5019 T5017 dobj 8,spanning
R3186 T5020 T5019 punct ", ",8
R3187 T5021 T5019 conj 9,8
R3188 T5022 T5021 punct ", ",9
R3189 T5023 T5021 cc and,9
R3190 T5024 T5021 conj 10,9
R3191 T5025 T5017 cc and,spanning
R3192 T5026 T5017 conj flanking,spanning
R3193 T5027 T5028 compound intron,sequences
R3194 T5028 T5026 dobj sequences,flanking
R3195 T5029 T5011 punct ", ",fragment
R3196 T5030 T5031 advmod originally,obtained
R3197 T5031 T5011 acl obtained,fragment
R3198 T5032 T5031 prep from,obtained
R3199 T5033 T5034 det a,library
R3200 T5034 T5032 pobj library,from
R3201 T5035 T5036 nmod Lambda,FIXII
R3202 T5036 T5034 nmod FIXII,library
R3203 T5037 T5034 nmod 129Sv,library
R3204 T5038 T5034 nmod mouse,library
R3205 T5039 T5034 amod genomic,library
R3206 T5040 T5041 punct [,25
R3207 T5041 T5034 parataxis 25,library
R3208 T5042 T5041 nummod 24,25
R3209 T5043 T5041 punct ",",25
R3210 T5044 T5041 punct ],25
R3211 T5045 T5008 punct ", ",cloned
R3212 T5046 T5008 auxpass was,cloned
R3213 T5047 T5008 advmod directionally,cloned
R3214 T5048 T5008 prep into,cloned
R3215 T5049 T5050 det the,sites
R3216 T5050 T5048 pobj sites,into
R3217 T5051 T5050 nmod NotI,sites
R3218 T5052 T5051 cc and,NotI
R3219 T5053 T5051 conj XhoI,NotI
R3220 T5054 T5050 prep of,sites
R3221 T5055 T5054 pobj pGEM11Zf,of
R3222 T5056 T5057 punct (,+
R3223 T5057 T5055 punct +,pGEM11Zf
R3224 T5058 T5057 punct ),+
R3225 T5059 T5008 punct .,cloned
R3226 T5061 T5062 det The,vector
R3227 T5062 T5063 nsubjpass vector,digested
R3228 T5064 T5063 auxpass was,digested
R3229 T5065 T5063 prep with,digested
R3230 T5066 T5065 pobj BamHI,with
R3231 T5067 T5066 cc and,BamHI
R3232 T5068 T5066 conj EcoRI,BamHI
R3233 T5069 T5070 aux to,remove
R3234 T5070 T5063 advcl remove,digested
R3235 T5071 T5072 det a,fragment
R3236 T5072 T5070 dobj fragment,remove
R3237 T5073 T5074 nummod 1.3,kb
R3238 T5074 T5072 nmod kb,fragment
R3239 T5075 T5074 punct -,kb
R3240 T5076 T5077 nmod BamHI,EcoRI
R3241 T5077 T5072 nmod EcoRI,fragment
R3242 T5078 T5077 punct /,EcoRI
R3243 T5079 T5072 amod genomic,fragment
R3244 T5080 T5072 acl containing,fragment
R3245 T5081 T5080 dobj exon,containing
R3246 T5082 T5081 nummod 10,exon
R3247 T5083 T5080 cc and,containing
R3248 T5084 T5080 conj flanking,containing
R3249 T5085 T5086 compound intron,sequences
R3250 T5086 T5084 dobj sequences,flanking
R3251 T5087 T5063 punct .,digested
R3252 T5089 T5090 det The,vector
R3253 T5090 T5092 nsubjpass vector,purified
R3254 T5091 T5090 amod digested,vector
R3255 T5093 T5090 punct ", ",vector
R3256 T5094 T5090 prep without,vector
R3257 T5095 T5096 det the,fragment
R3258 T5096 T5094 pobj fragment,without
R3259 T5097 T5098 nummod 1.3,kb
R3260 T5098 T5096 nmod kb,fragment
R3261 T5099 T5098 punct -,kb
R3262 T5100 T5101 nmod BamHI,EcoRI
R3263 T5101 T5096 nmod EcoRI,fragment
R3264 T5102 T5101 punct /,EcoRI
R3265 T5103 T5096 amod genomic,fragment
R3266 T5104 T5092 punct ", ",purified
R3267 T5105 T5092 auxpass was,purified
R3268 T5106 T5092 prep by,purified
R3269 T5107 T5108 compound gel,purification
R3270 T5108 T5106 pobj purification,by
R3271 T5109 T5092 cc and,purified
R3272 T5110 T5092 conj recircularized,purified
R3273 T5111 T5110 prep by,recircularized
R3274 T5112 T5111 pobj ligation,by
R3275 T5113 T5110 advcl using,recircularized
R3276 T5114 T5115 nummod three,oligonucleotides
R3277 T5115 T5113 dobj oligonucleotides,using
R3278 T5116 T5115 punct : ,oligonucleotides
R3279 T5117 T5118 nummod 5,AATTGTCGACA
R3280 T5118 T5115 appos AATTGTCGACA,oligonucleotides
R3281 T5119 T5117 punct ′,5
R3282 T5120 T5118 punct -,AATTGTCGACA
R3283 T5121 T5118 punct -,AATTGTCGACA
R3284 T5122 T5118 nummod 3,AATTGTCGACA
R3285 T5123 T5118 punct ′,AATTGTCGACA
R3286 T5124 T5118 punct ;,AATTGTCGACA
R3287 T5125 T5126 nummod 5,GATCGTCGACA
R3288 T5126 T5118 conj GATCGTCGACA,AATTGTCGACA
R3289 T5127 T5125 punct ′,5
R3290 T5128 T5126 punct -,GATCGTCGACA
R3291 T5129 T5126 nummod 3,GATCGTCGACA
R3292 T5130 T5126 punct ′,GATCGTCGACA
R3293 T5131 T5126 punct ;,GATCGTCGACA
R3294 T5132 T5126 cc and,GATCGTCGACA
R3295 T5133 T5134 nummod 5,TCGATGTCGAC
R3296 T5134 T5126 conj TCGATGTCGAC,GATCGTCGACA
R3297 T5135 T5133 punct ′,5
R3298 T5136 T5134 punct -,TCGATGTCGAC
R3299 T5137 T5134 punct -,TCGATGTCGAC
R3300 T5138 T5134 nummod 3,TCGATGTCGAC
R3301 T5139 T5134 punct ′,TCGATGTCGAC
R3302 T5140 T5092 punct .,purified
R3303 T5142 T5143 det The,vector
R3304 T5143 T5145 nsubj vector,contained
R3305 T5144 T5143 amod recircularized,vector
R3306 T5146 T5143 punct ", ",vector
R3307 T5147 T5143 acl resulting,vector
R3308 T5148 T5147 prep from,resulting
R3309 T5149 T5150 det the,ligation
R3310 T5150 T5148 pobj ligation,from
R3311 T5151 T5150 prep of,ligation
R3312 T5152 T5153 det the,arm
R3313 T5153 T5151 pobj arm,of
R3314 T5154 T5153 amod long,arm
R3315 T5155 T5150 prep to,ligation
R3316 T5156 T5157 det the,arm
R3317 T5157 T5155 pobj arm,to
R3318 T5158 T5157 amod short,arm
R3319 T5159 T5150 prep of,ligation
R3320 T5160 T5159 pobj homology,of
R3321 T5161 T5145 punct ", ",contained
R3322 T5162 T5163 det a,site
R3323 T5163 T5145 dobj site,contained
R3324 T5164 T5163 amod unique,site
R3325 T5165 T5163 compound SalI,site
R3326 T5166 T5167 advmod where,deleted
R3327 T5167 T5163 relcl deleted,site
R3328 T5168 T5169 det the,region
R3329 T5169 T5167 nsubjpass region,deleted
R3330 T5170 T5171 nummod 1.3,kb
R3331 T5171 T5169 nmod kb,region
R3332 T5172 T5171 punct -,kb
R3333 T5173 T5169 nmod exon,region
R3334 T5174 T5173 nummod 10,exon
R3335 T5175 T5167 auxpass was,deleted
R3336 T5176 T5145 punct .,contained
R3337 T5281 T5280 prep of,Generation
R3338 T5282 T5283 npadvmod MCAD,deficient
R3339 T5283 T5285 amod deficient,mice
R3340 T5284 T5283 punct -,deficient
R3341 T5285 T5281 pobj mice,of
R3342 T5286 T5280 punct .,Generation
R3343 T5288 T5289 det The,vector
R3344 T5289 T5292 nsubjpass vector,linearized
R3345 T5290 T5289 compound Acadm,vector
R3346 T5291 T5289 compound insertion,vector
R3347 T5293 T5292 auxpass was,linearized
R3348 T5294 T5292 prep by,linearized
R3349 T5295 T5296 compound SalI,digestion
R3350 T5296 T5294 pobj digestion,by
R3351 T5297 T5296 punct ", ",digestion
R3352 T5298 T5299 det the,site
R3353 T5299 T5296 appos site,digestion
R3354 T5300 T5299 prep of,site
R3355 T5301 T5302 det the,deletion
R3356 T5302 T5300 pobj deletion,of
R3357 T5303 T5304 nummod 1.3,kb
R3358 T5304 T5302 nmod kb,deletion
R3359 T5305 T5304 punct -,kb
R3360 T5306 T5307 amod genomic,fragment
R3361 T5307 T5302 compound fragment,deletion
R3362 T5308 T5292 punct ", ",linearized
R3363 T5309 T5292 cc and,linearized
R3364 T5310 T5292 conj electroporated,linearized
R3365 T5311 T5310 prep into,electroporated
R3366 T5312 T5313 nmod E14,cells
R3367 T5313 T5311 pobj cells,into
R3368 T5314 T5312 punct –,E14
R3369 T5315 T5312 nummod 1,E14
R3370 T5316 T5313 compound ES,cells
R3371 T5317 T5318 punct (,gift
R3372 T5318 T5313 parataxis gift,cells
R3373 T5319 T5318 det a,gift
R3374 T5320 T5318 amod kind,gift
R3375 T5321 T5318 prep from,gift
R3376 T5322 T5323 compound R.,Kuhn
R3377 T5323 T5321 pobj Kuhn,from
R3378 T5324 T5318 punct ),gift
R3379 T5325 T5313 punct ", ",cells
R3380 T5326 T5313 acl derived,cells
R3381 T5327 T5326 prep from,derived
R3382 T5328 T5329 compound 129P2,mice
R3383 T5329 T5327 pobj mice,from
R3384 T5330 T5292 punct .,linearized
R3385 T5332 T5333 advmod Correctly,targeted
R3386 T5333 T5334 amod targeted,vector
R3387 T5334 T5337 nsubjpass vector,designed
R3388 T5335 T5334 compound Acadm,vector
R3389 T5336 T5334 compound insertion,vector
R3390 T5338 T5337 auxpass was,designed
R3391 T5339 T5340 aux to,undergo
R3392 T5340 T5337 advcl undergo,designed
R3393 T5341 T5342 compound gap,repair
R3394 T5342 T5340 dobj repair,undergo
R3395 T5343 T5342 prep of,repair
R3396 T5344 T5345 det the,deletion
R3397 T5345 T5343 pobj deletion,of
R3398 T5346 T5347 nummod 1.3,kb
R3399 T5347 T5345 compound kb,deletion
R3400 T5348 T5347 punct -,kb
R3401 T5349 T5340 prep upon,undergo
R3402 T5350 T5351 amod double,stranded
R3403 T5351 T5352 amod stranded,break
R3404 T5352 T5354 compound break,repair
R3405 T5353 T5352 punct –,break
R3406 T5354 T5349 pobj repair,upon
R3407 T5355 T5356 punct [,26
R3408 T5356 T5354 parataxis 26,repair
R3409 T5357 T5356 punct ],26
R3410 T5358 T5354 prep during,repair
R3411 T5359 T5360 amod homologous,recombination
R3412 T5360 T5358 pobj recombination,during
R3413 T5361 T5337 punct .,designed
R3414 T5363 T5364 compound Southern,blot
R3415 T5364 T5365 compound blot,analysis
R3416 T5365 T5366 nsubjpass analysis,conducted
R3417 T5367 T5366 auxpass was,conducted
R3418 T5368 T5369 aux to,confirm
R3419 T5369 T5366 advcl confirm,conducted
R3420 T5370 T5371 amod homologous,recombination
R3421 T5371 T5369 dobj recombination,confirm
R3422 T5372 T5366 punct .,conducted
R3423 T5374 T5375 amod Genomic,DNA
R3424 T5375 T5376 nsubjpass DNA,digested
R3425 T5377 T5375 prep from,DNA
R3426 T5378 T5379 npadvmod G418,resistant
R3427 T5379 T5380 amod resistant,colonies
R3428 T5380 T5377 pobj colonies,from
R3429 T5381 T5380 compound ES,colonies
R3430 T5382 T5376 auxpass was,digested
R3431 T5383 T5376 prep with,digested
R3432 T5384 T5383 pobj EcoRI,with
R3433 T5385 T5376 punct ", ",digested
R3434 T5386 T5376 conj electrophoresed,digested
R3435 T5387 T5386 punct ", ",electrophoresed
R3436 T5388 T5386 conj blotted,electrophoresed
R3437 T5389 T5388 punct ", ",blotted
R3438 T5390 T5388 cc and,blotted
R3439 T5391 T5388 conj probed,blotted
R3440 T5392 T5391 prep with,probed
R3441 T5393 T5394 det an,probe
R3442 T5394 T5392 pobj probe,with
R3443 T5395 T5396 nummod 850,bp
R3444 T5396 T5394 compound bp,probe
R3445 T5397 T5396 punct -,bp
R3446 T5398 T5399 punct (,A
R3447 T5399 T5394 parataxis A,probe
R3448 T5400 T5399 compound probe,A
R3449 T5401 T5399 punct ),A
R3450 T5402 T5394 acl generated,probe
R3451 T5403 T5402 prep by,generated
R3452 T5404 T5403 pobj PCR,by
R3453 T5405 T5402 prep from,generated
R3454 T5406 T5407 compound Acadm,exon
R3455 T5407 T5405 pobj exon,from
R3456 T5408 T5407 nummod 10,exon
R3457 T5409 T5405 prep to,from
R3458 T5410 T5409 pobj intron,to
R3459 T5411 T5410 nummod 10,intron
R3460 T5412 T5376 punct .,digested
R3461 T5414 T5415 det This,fragment
R3462 T5415 T5417 nsubj fragment,is
R3463 T5416 T5415 compound DNA,fragment
R3464 T5418 T5417 neg not,is
R3465 T5419 T5417 acomp present,is
R3466 T5420 T5417 prep within,is
R3467 T5421 T5422 compound Acadm,vector
R3468 T5422 T5420 pobj vector,within
R3469 T5423 T5422 compound insertion,vector
R3470 T5424 T5417 cc and,is
R3471 T5425 T5426 auxpass was,expected
R3472 T5426 T5417 conj expected,is
R3473 T5427 T5428 aux to,hybridize
R3474 T5428 T5426 xcomp hybridize,expected
R3475 T5429 T5428 prep to,hybridize
R3476 T5430 T5431 det a,fragment
R3477 T5431 T5429 pobj fragment,to
R3478 T5432 T5433 nummod 13.2,kb
R3479 T5433 T5431 nmod kb,fragment
R3480 T5434 T5433 punct -,kb
R3481 T5435 T5431 amod genomic,fragment
R3482 T5436 T5431 compound DNA,fragment
R3483 T5437 T5428 prep upon,hybridize
R3484 T5438 T5439 amod homologous,recombination
R3485 T5439 T5437 pobj recombination,upon
R3486 T5440 T5417 punct .,is
R3487 T5442 T5443 advmod Correctly,targeted
R3488 T5443 T5444 amod targeted,clones
R3489 T5444 T5447 nsubjpass clones,microinjected
R3490 T5445 T5446 compound ES,cell
R3491 T5446 T5444 compound cell,clones
R3492 T5448 T5447 auxpass were,microinjected
R3493 T5449 T5447 prep into,microinjected
R3494 T5450 T5451 compound B6,blastocysts
R3495 T5451 T5449 pobj blastocysts,into
R3496 T5452 T5453 aux to,generate
R3497 T5453 T5447 advcl generate,microinjected
R3498 T5454 T5455 amod chimeric,mice
R3499 T5455 T5453 dobj mice,generate
R3500 T5456 T5447 punct .,microinjected
R3501 T5458 T5459 amod Chimeric,mice
R3502 T5459 T5460 nsubjpass mice,backcrossed
R3503 T5461 T5460 auxpass were,backcrossed
R3504 T5462 T5460 advmod subsequently,backcrossed
R3505 T5463 T5460 prep to,backcrossed
R3506 T5464 T5465 nmod B6,mice
R3507 T5465 T5463 pobj mice,to
R3508 T5466 T5464 cc and,B6
R3509 T5467 T5464 conj 129P2,B6
R3510 T5468 T5469 aux to,produce
R3511 T5469 T5460 advcl produce,backcrossed
R3512 T5470 T5471 npadvmod gene,targeted
R3513 T5471 T5473 amod targeted,mice
R3514 T5472 T5471 punct -,targeted
R3515 T5473 T5469 dobj mice,produce
R3516 T5474 T5473 appos AcadmtmUab1,mice
R3517 T5475 T5474 punct /,AcadmtmUab1
R3518 T5476 T5474 punct +,AcadmtmUab1
R3519 T5477 T5478 punct (,MCAD
R3520 T5478 T5474 parataxis MCAD,AcadmtmUab1
R3521 T5479 T5478 punct +,MCAD
R3522 T5480 T5478 punct /,MCAD
R3523 T5481 T5478 punct −,MCAD
R3524 T5482 T5478 punct ),MCAD
R3525 T5483 T5473 cc and,mice
R3526 T5484 T5485 advmod eventually,mice
R3527 T5485 T5473 conj mice,mice
R3528 T5486 T5487 nmod AcadmtmUab1,tm1Uab
R3529 T5487 T5485 nmod tm1Uab,mice
R3530 T5488 T5487 punct /,tm1Uab
R3531 T5489 T5485 nmod MCAD,mice
R3532 T5490 T5485 punct −,mice
R3533 T5491 T5485 punct /,mice
R3534 T5492 T5485 punct −,mice
R3535 T5493 T5494 punct (,B6
R3536 T5494 T5485 parataxis B6,mice
R3537 T5495 T5494 punct ;,B6
R3538 T5496 T5494 nummod 129,B6
R3539 T5497 T5494 punct ),B6
R3540 T5498 T5460 punct .,backcrossed
R3541 T5500 T5501 nsubj Mice,were
R3542 T5502 T5501 acomp negative,were
R3543 T5503 T5502 prep for,negative
R3544 T5504 T5505 compound mouse,pathogens
R3545 T5505 T5503 pobj pathogens,for
R3546 T5506 T5501 prep based,were
R3547 T5507 T5506 prep on,based
R3548 T5508 T5509 amod serological,assays
R3549 T5509 T5507 pobj assays,on
R3550 T5510 T5509 prep for,assays
R3551 T5511 T5512 nummod ten,viruses
R3552 T5512 T5510 pobj viruses,for
R3553 T5513 T5512 amod different,viruses
R3554 T5514 T5509 punct ", ",assays
R3555 T5515 T5516 amod aerobic,cultures
R3556 T5516 T5509 conj cultures,assays
R3557 T5517 T5516 amod bacterial,cultures
R3558 T5518 T5516 prep of,cultures
R3559 T5519 T5518 pobj nasopharynx,of
R3560 T5520 T5519 cc and,nasopharynx
R3561 T5521 T5519 conj cecum,nasopharynx
R3562 T5522 T5516 punct ", ",cultures
R3563 T5523 T5516 conj examinations,cultures
R3564 T5524 T5523 prep for,examinations
R3565 T5525 T5526 nmod endo,ectoparasites
R3566 T5526 T5524 pobj ectoparasites,for
R3567 T5527 T5526 punct -,ectoparasites
R3568 T5528 T5526 cc and,ectoparasites
R3569 T5529 T5523 punct ", ",examinations
R3570 T5530 T5523 cc and,examinations
R3571 T5531 T5523 conj histopathology,examinations
R3572 T5532 T5531 prep of,histopathology
R3573 T5533 T5534 det all,organs
R3574 T5534 T5532 pobj organs,of
R3575 T5535 T5534 amod major,organs
R3576 T5536 T5501 punct .,were
R3577 T5679 T5680 compound RNA,analysis
R3578 T5681 T5680 punct .,analysis
R3579 T5683 T5684 amod Total,RNA
R3580 T5684 T5685 nsubjpass RNA,isolated
R3581 T5686 T5685 auxpass was,isolated
R3582 T5687 T5685 prep from,isolated
R3583 T5688 T5689 det the,heart
R3584 T5689 T5687 pobj heart,from
R3585 T5690 T5689 prep of,heart
R3586 T5691 T5692 nummod 30,day
R3587 T5692 T5693 npadvmod day,old
R3588 T5693 T5694 amod old,mice
R3589 T5694 T5690 pobj mice,of
R3590 T5695 T5694 nmod MCAD,mice
R3591 T5696 T5695 punct +,MCAD
R3592 T5697 T5695 punct /,MCAD
R3593 T5698 T5695 punct +,MCAD
R3594 T5699 T5695 punct ", ",MCAD
R3595 T5700 T5695 conj MCAD,MCAD
R3596 T5701 T5700 punct +,MCAD
R3597 T5702 T5700 punct /,MCAD
R3598 T5703 T5700 punct −,MCAD
R3599 T5704 T5700 punct ", ",MCAD
R3600 T5705 T5700 cc and,MCAD
R3601 T5706 T5700 conj MCAD,MCAD
R3602 T5707 T5706 punct −,MCAD
R3603 T5708 T5706 punct /,MCAD
R3604 T5709 T5706 punct −,MCAD
R3605 T5710 T5685 prep by,isolated
R3606 T5711 T5712 amod standard,techniques
R3607 T5712 T5710 pobj techniques,by
R3608 T5713 T5685 advcl using,isolated
R3609 T5714 T5715 compound guanidinium,method
R3610 T5715 T5713 dobj method,using
R3611 T5716 T5715 compound thiocyanate,method
R3612 T5717 T5718 punct [,27
R3613 T5718 T5685 parataxis 27,isolated
R3614 T5719 T5718 punct ],27
R3615 T5720 T5685 punct .,isolated
R3616 T5722 T5723 amod Reverse,transcription
R3617 T5723 T5724 nsubjpass transcription,performed
R3618 T5725 T5724 auxpass was,performed
R3619 T5726 T5724 advcl using,performed
R3620 T5727 T5728 amod random,oligonucleotides
R3621 T5728 T5726 dobj oligonucleotides,using
R3622 T5729 T5730 mark as,recommended
R3623 T5730 T5724 advcl recommended,performed
R3624 T5731 T5730 agent by,recommended
R3625 T5732 T5733 det the,manufacturer
R3626 T5733 T5731 pobj manufacturer,by
R3627 T5734 T5735 punct (,Clontech
R3628 T5735 T5733 parataxis Clontech,manufacturer
R3629 T5736 T5735 punct ", ",Clontech
R3630 T5737 T5738 compound Mountain,View
R3631 T5738 T5735 npadvmod View,Clontech
R3632 T5739 T5735 punct ", ",Clontech
R3633 T5740 T5735 npadvmod California,Clontech
R3634 T5741 T5735 punct ", ",Clontech
R3635 T5742 T5743 compound United,States
R3636 T5743 T5735 npadvmod States,Clontech
R3637 T5744 T5735 punct ),Clontech
R3638 T5745 T5724 punct .,performed
R3639 T5747 T5748 nsubjpass PCR,performed
R3640 T5749 T5748 auxpass was,performed
R3641 T5750 T5748 advmod subsequently,performed
R3642 T5751 T5748 advcl using,performed
R3643 T5752 T5751 dobj oligonucleotides,using
R3644 T5753 T5752 amod specific,oligonucleotides
R3645 T5754 T5753 prep to,specific
R3646 T5755 T5754 pobj exon,to
R3647 T5756 T5755 nummod 7,exon
R3648 T5757 T5755 cc and,exon
R3649 T5758 T5755 conj exon,exon
R3650 T5759 T5758 nummod 11,exon
R3651 T5760 T5755 prep of,exon
R3652 T5761 T5760 pobj Acadm,of
R3653 T5762 T5748 punct .,performed
R3654 T5764 T5765 compound PCR,amplifications
R3655 T5765 T5766 nsubjpass amplifications,performed
R3656 T5767 T5766 auxpass were,performed
R3657 T5768 T5769 mark as,described
R3658 T5769 T5766 advcl described,performed
R3659 T5770 T5769 advmod above,described
R3660 T5771 T5766 punct .,performed
R3661 T5773 T5774 compound PCR,fragments
R3662 T5774 T5775 nsubjpass fragments,sequenced
R3663 T5776 T5775 auxpass were,sequenced
R3664 T5777 T5775 advmod subsequently,sequenced
R3665 T5778 T5775 prep after,sequenced
R3666 T5779 T5778 pcomp subcloning,after
R3667 T5780 T5779 prep into,subcloning
R3668 T5781 T5782 compound pGEM,T
R3669 T5782 T5784 compound T,vector
R3670 T5783 T5782 punct -,T
R3671 T5784 T5780 pobj vector,into
R3672 T5785 T5784 compound Easy,vector
R3673 T5786 T5787 punct (,Promega
R3674 T5787 T5784 parataxis Promega,vector
R3675 T5788 T5787 punct ", ",Promega
R3676 T5789 T5787 npadvmod Madison,Promega
R3677 T5790 T5787 punct ", ",Promega
R3678 T5791 T5787 npadvmod Wisconsin,Promega
R3679 T5792 T5787 punct ", ",Promega
R3680 T5793 T5794 compound United,States
R3681 T5794 T5787 npadvmod States,Promega
R3682 T5795 T5787 punct ),Promega
R3683 T5796 T5775 punct .,sequenced
R3684 T5798 T5799 prep In,performed
R3685 T5800 T5798 pobj order,In
R3686 T5801 T5802 aux to,determine
R3687 T5802 T5800 acl determine,order
R3688 T5803 T5804 det the,extent
R3689 T5804 T5802 dobj extent,determine
R3690 T5805 T5804 prep of,extent
R3691 T5806 T5807 compound Acadm,mRNA
R3692 T5807 T5805 pobj mRNA,of
R3693 T5808 T5807 acl expressed,mRNA
R3694 T5809 T5808 prep from,expressed
R3695 T5810 T5811 det the,mice
R3696 T5811 T5809 pobj mice,from
R3697 T5812 T5811 nmod MCAD,mice
R3698 T5813 T5812 punct −,MCAD
R3699 T5814 T5812 punct /,MCAD
R3700 T5815 T5812 punct −,MCAD
R3701 T5816 T5799 punct ", ",performed
R3702 T5817 T5818 compound northern,blot
R3703 T5818 T5819 compound blot,analysis
R3704 T5819 T5799 nsubjpass analysis,performed
R3705 T5820 T5799 auxpass was,performed
R3706 T5821 T5799 punct .,performed
R3707 T5823 T5824 amod Total,RNA
R3708 T5824 T5825 nsubjpass RNA,isolated
R3709 T5826 T5825 auxpass was,isolated
R3710 T5827 T5825 prep from,isolated
R3711 T5828 T5827 pobj heart,from
R3712 T5829 T5828 punct ", ",heart
R3713 T5830 T5828 conj liver,heart
R3714 T5831 T5830 punct ", ",liver
R3715 T5832 T5833 amod brown,fat
R3716 T5833 T5830 conj fat,liver
R3717 T5834 T5833 punct ", ",fat
R3718 T5835 T5833 conj brain,fat
R3719 T5836 T5835 punct ", ",brain
R3720 T5837 T5835 conj kidney,brain
R3721 T5838 T5837 punct ", ",kidney
R3722 T5839 T5840 amod skeletal,muscle
R3723 T5840 T5837 conj muscle,kidney
R3724 T5841 T5840 punct ", ",muscle
R3725 T5842 T5843 amod white,fat
R3726 T5843 T5840 conj fat,muscle
R3727 T5844 T5843 punct ", ",fat
R3728 T5845 T5843 cc and,fat
R3729 T5846 T5843 conj testes,fat
R3730 T5847 T5828 prep of,heart
R3731 T5848 T5849 nummod 3,mo
R3732 T5849 T5851 npadvmod mo,old
R3733 T5850 T5849 punct -,mo
R3734 T5851 T5853 amod old,mice
R3735 T5852 T5851 punct -,old
R3736 T5853 T5847 pobj mice,of
R3737 T5854 T5853 nmod MCAD,mice
R3738 T5855 T5854 punct +,MCAD
R3739 T5856 T5854 punct /,MCAD
R3740 T5857 T5854 punct +,MCAD
R3741 T5858 T5854 cc and,MCAD
R3742 T5859 T5854 conj MCAD,MCAD
R3743 T5860 T5859 punct −,MCAD
R3744 T5861 T5859 punct /,MCAD
R3745 T5862 T5859 punct −,MCAD
R3746 T5863 T5825 advcl using,isolated
R3747 T5864 T5865 det the,Kit
R3748 T5865 T5863 dobj Kit,using
R3749 T5866 T5865 compound Ultraspec,Kit
R3750 T5867 T5868 compound RNA,Isolation
R3751 T5868 T5865 compound Isolation,Kit
R3752 T5869 T5870 punct (,Laboratories
R3753 T5870 T5865 parataxis Laboratories,Kit
R3754 T5871 T5870 compound BIOTEX,Laboratories
R3755 T5872 T5870 punct ", ",Laboratories
R3756 T5873 T5870 amod Inc.,Laboratories
R3757 T5874 T5870 punct ", ",Laboratories
R3758 T5875 T5870 npadvmod Houston,Laboratories
R3759 T5876 T5870 punct ", ",Laboratories
R3760 T5877 T5870 npadvmod Texas,Laboratories
R3761 T5878 T5870 punct ", ",Laboratories
R3762 T5879 T5880 compound United,States
R3763 T5880 T5870 npadvmod States,Laboratories
R3764 T5881 T5870 punct ),Laboratories
R3765 T5882 T5863 prep as,using
R3766 T5883 T5882 prep per,as
R3767 T5884 T5885 poss manufacturer,protocol
R3768 T5885 T5883 pobj protocol,per
R3769 T5886 T5884 case 's,manufacturer
R3770 T5887 T5825 punct .,isolated
R3771 T5889 T5890 nummod Ten,μg
R3772 T5890 T5891 nsubjpass μg,loaded
R3773 T5892 T5890 prep of,μg
R3774 T5893 T5894 amod total,RNA
R3775 T5894 T5892 pobj RNA,of
R3776 T5895 T5890 prep from,μg
R3777 T5896 T5897 det each,sample
R3778 T5897 T5895 pobj sample,from
R3779 T5898 T5891 auxpass was,loaded
R3780 T5899 T5891 prep onto,loaded
R3781 T5900 T5901 det a,gel
R3782 T5901 T5899 pobj gel,onto
R3783 T5902 T5903 nummod 0.8,%
R3784 T5903 T5904 compound %,formaldehyde
R3785 T5904 T5901 compound formaldehyde,gel
R3786 T5905 T5904 compound agarose,formaldehyde
R3787 T5906 T5904 punct -,formaldehyde
R3788 T5907 T5891 punct ", ",loaded
R3789 T5908 T5891 conj transferred,loaded
R3790 T5909 T5908 prep to,transferred
R3791 T5910 T5911 compound nitrocellulose,filter
R3792 T5911 T5909 pobj filter,to
R3793 T5912 T5913 punct (,Corp.
R3794 T5913 T5911 parataxis Corp.,filter
R3795 T5914 T5915 compound Hybond,N
R3796 T5915 T5913 dep N,Corp.
R3797 T5916 T5913 punct ;,Corp.
R3798 T5917 T5913 compound GE,Corp.
R3799 T5918 T5913 compound Healthcare,Corp.
R3800 T5919 T5913 compound Amersham,Corp.
R3801 T5920 T5913 compound Biosciences,Corp.
R3802 T5921 T5913 punct ", ",Corp.
R3803 T5922 T5913 npadvmod Piscataway,Corp.
R3804 T5923 T5913 punct ", ",Corp.
R3805 T5924 T5925 compound New,Jersey
R3806 T5925 T5913 npadvmod Jersey,Corp.
R3807 T5926 T5913 punct ", ",Corp.
R3808 T5927 T5928 compound United,States
R3809 T5928 T5913 npadvmod States,Corp.
R3810 T5929 T5913 punct ),Corp.
R3811 T5930 T5908 punct ", ",transferred
R3812 T5931 T5908 cc and,transferred
R3813 T5932 T5908 conj hybridized,transferred
R3814 T5933 T5932 prep with,hybridized
R3815 T5934 T5935 npadvmod 32P,radiolabeled
R3816 T5935 T5937 amod radiolabeled,probe
R3817 T5936 T5935 punct -,radiolabeled
R3818 T5937 T5933 pobj probe,with
R3819 T5938 T5939 amod full,length
R3820 T5939 T5937 compound length,probe
R3821 T5940 T5939 punct -,length
R3822 T5941 T5937 compound mouse,probe
R3823 T5942 T5937 compound Acadm,probe
R3824 T5943 T5937 compound cDNA,probe
R3825 T5944 T5932 advcl using,hybridized
R3826 T5945 T5946 amod standard,procedures
R3827 T5946 T5944 dobj procedures,using
R3828 T5947 T5948 punct [,28
R3829 T5948 T5932 parataxis 28,hybridized
R3830 T5949 T5948 punct ],28
R3831 T5950 T5891 punct .,loaded
R3832 T5952 T5953 nsubjpass Hybridizations,performed
R3833 T5954 T5953 auxpass were,performed
R3834 T5955 T5953 prep under,performed
R3835 T5956 T5957 advmod highly,stringent
R3836 T5957 T5958 amod stringent,conditions
R3837 T5958 T5955 pobj conditions,under
R3838 T5959 T5960 punct (,°C
R3839 T5960 T5955 parataxis °C,under
R3840 T5961 T5960 nummod 42,°C
R3841 T5962 T5960 prep in,°C
R3842 T5963 T5964 nummod 2,SSC
R3843 T5964 T5962 pobj SSC,in
R3844 T5965 T5963 punct ×,2
R3845 T5966 T5960 punct ", ",°C
R3846 T5967 T5968 nummod 50,%
R3847 T5968 T5969 compound %,formamide
R3848 T5969 T5960 conj formamide,°C
R3849 T5970 T5969 punct ", ",formamide
R3850 T5971 T5972 nummod 10,%
R3851 T5972 T5973 compound %,sulfate
R3852 T5973 T5969 conj sulfate,formamide
R3853 T5974 T5973 compound dextran,sulfate
R3854 T5975 T5973 punct ", ",sulfate
R3855 T5976 T5977 nummod 5,reagent
R3856 T5977 T5973 conj reagent,sulfate
R3857 T5978 T5976 punct ×,5
R3858 T5979 T5977 poss Denhardt,reagent
R3859 T5980 T5979 case 's,Denhardt
R3860 T5981 T5977 punct ", ",reagent
R3861 T5982 T5983 nummod 1,%
R3862 T5983 T5984 compound %,SDS
R3863 T5984 T5977 conj SDS,reagent
R3864 T5985 T5984 punct ", ",SDS
R3865 T5986 T5984 cc and,SDS
R3866 T5987 T5988 compound salmon,sperm
R3867 T5988 T5989 compound sperm,DNA
R3868 T5989 T5984 conj DNA,SDS
R3869 T5990 T5960 punct ),°C
R3870 T5991 T5953 prep for,performed
R3871 T5992 T5993 nummod 18,h
R3872 T5993 T5991 pobj h,for
R3873 T5994 T5953 punct .,performed
R3874 T5996 T5997 det The,filter
R3875 T5997 T5999 nsubj filter,washed
R3876 T5998 T5997 amod hybridized,filter
R3877 T6000 T5999 aux was,washed
R3878 T6001 T6002 nummod two,times
R3879 T6002 T5999 npadvmod times,washed
R3880 T6003 T5999 prep in,washed
R3881 T6004 T6005 nummod 4,SSC
R3882 T6005 T6003 pobj SSC,in
R3883 T6006 T6004 punct ×,4
R3884 T6007 T6005 punct ", ",SSC
R3885 T6008 T6009 nummod 0.1,%
R3886 T6009 T6010 compound %,SDS
R3887 T6010 T6005 appos SDS,SSC
R3888 T6011 T5999 cc and,washed
R3889 T6012 T6013 nummod two,times
R3890 T6013 T5999 conj times,washed
R3891 T6014 T6013 prep in,times
R3892 T6015 T6016 nummod 2,SSC
R3893 T6016 T6014 pobj SSC,in
R3894 T6017 T6015 punct ×,2
R3895 T6018 T6016 punct ;,SSC
R3896 T6019 T6020 nummod 0.1,%
R3897 T6020 T6021 compound %,SDS
R3898 T6021 T6016 appos SDS,SSC
R3899 T6022 T5999 prep at,washed
R3900 T6023 T6024 nummod 55,°C
R3901 T6024 T6022 pobj °C,at
R3902 T6025 T5999 prep for,washed
R3903 T6026 T6027 nummod 1,h
R3904 T6027 T6025 pobj h,for
R3905 T6028 T5999 punct .,washed
R3906 T6030 T6031 det The,filter
R3907 T6031 T6032 nsubjpass filter,exposed
R3908 T6033 T6032 auxpass was,exposed
R3909 T6034 T6032 prep to,exposed
R3910 T6035 T6036 amod autoradiographic,film
R3911 T6036 T6034 pobj film,to
R3912 T6037 T6038 punct (,Biosciences
R3913 T6038 T6036 parataxis Biosciences,film
R3914 T6039 T6040 compound Hyperfilm,MP
R3915 T6040 T6038 dep MP,Biosciences
R3916 T6041 T6038 punct ;,Biosciences
R3917 T6042 T6038 compound GE,Biosciences
R3918 T6043 T6038 compound Healthcare,Biosciences
R3919 T6044 T6038 compound Amersham,Biosciences
R3920 T6045 T6038 punct ", ",Biosciences
R3921 T6046 T6038 npadvmod Piscataway,Biosciences
R3922 T6047 T6038 punct ", ",Biosciences
R3923 T6048 T6049 compound New,Jersey
R3924 T6049 T6038 npadvmod Jersey,Biosciences
R3925 T6050 T6038 punct ", ",Biosciences
R3926 T6051 T6052 compound United,States
R3927 T6052 T6038 npadvmod States,Biosciences
R3928 T6053 T6038 punct ),Biosciences
R3929 T6054 T6032 punct .,exposed
R3930 T6056 T6057 amod Replicate,gels
R3931 T6057 T6061 nsubjpass gels,stained
R3932 T6058 T6059 compound agarose,formaldehyde
R3933 T6059 T6057 compound formaldehyde,gels
R3934 T6060 T6059 punct -,formaldehyde
R3935 T6062 T6061 auxpass were,stained
R3936 T6063 T6061 prep by,stained
R3937 T6064 T6065 compound ethidium,bromide
R3938 T6065 T6063 pobj bromide,by
R3939 T6066 T6067 aux to,verify
R3940 T6067 T6061 advcl verify,stained
R3941 T6068 T6069 amod equal,loading
R3942 T6069 T6067 dobj loading,verify
R3943 T6070 T6069 compound RNA,loading
R3944 T6071 T6061 punct .,stained
R3945 T6221 T6222 compound Immunoblot,analysis
R3946 T6223 T6222 prep of,analysis
R3947 T6224 T6225 compound MCAD,protein
R3948 T6225 T6223 pobj protein,of
R3949 T6226 T6222 punct .,analysis
R3950 T6228 T6229 aux To,evaluate
R3951 T6229 T6230 advcl evaluate,frozen
R3952 T6231 T6232 det the,quantity
R3953 T6232 T6229 dobj quantity,evaluate
R3954 T6233 T6232 prep of,quantity
R3955 T6234 T6235 compound MCAD,protein
R3956 T6235 T6233 pobj protein,of
R3957 T6236 T6229 prep in,evaluate
R3958 T6237 T6238 compound mouse,tissue
R3959 T6238 T6236 pobj tissue,in
R3960 T6239 T6230 punct ", ",frozen
R3961 T6240 T6241 compound liver,samples
R3962 T6241 T6230 nsubjpass samples,frozen
R3963 T6242 T6241 prep from,samples
R3964 T6243 T6244 quantmod 6,8
R3965 T6244 T6246 nummod 8,wk
R3966 T6245 T6244 punct –,8
R3967 T6246 T6248 npadvmod wk,old
R3968 T6247 T6246 punct -,wk
R3969 T6248 T6250 amod old,mice
R3970 T6249 T6248 punct -,old
R3971 T6250 T6242 pobj mice,from
R3972 T6251 T6250 nmod MCAD,mice
R3973 T6252 T6251 punct +,MCAD
R3974 T6253 T6251 punct /,MCAD
R3975 T6254 T6251 punct +,MCAD
R3976 T6255 T6256 punct (,1
R3977 T6256 T6251 parataxis 1,MCAD
R3978 T6257 T6256 nsubj n,1
R3979 T6258 T6256 punct =,1
R3980 T6259 T6256 punct ),1
R3981 T6260 T6251 cc and,MCAD
R3982 T6261 T6251 conj MCAD,MCAD
R3983 T6262 T6261 punct −,MCAD
R3984 T6263 T6261 punct /,MCAD
R3985 T6264 T6261 punct −,MCAD
R3986 T6265 T6266 punct (,3
R3987 T6266 T6261 parataxis 3,MCAD
R3988 T6267 T6266 nsubj n,3
R3989 T6268 T6266 punct =,3
R3990 T6269 T6266 punct ),3
R3991 T6270 T6230 auxpass were,frozen
R3992 T6271 T6230 advmod immediately,frozen
R3993 T6272 T6230 prep in,frozen
R3994 T6273 T6274 amod liquid,nitrogen
R3995 T6274 T6272 pobj nitrogen,in
R3996 T6275 T6230 cc and,frozen
R3997 T6276 T6230 conj stored,frozen
R3998 T6277 T6276 prep at,stored
R3999 T6278 T6279 punct −,80
R4000 T6279 T6280 nummod 80,°C
R4001 T6280 T6277 pobj °C,at
R4002 T6281 T6230 punct .,frozen
R4003 T6283 T6284 prep For,homogenized
R4004 T6285 T6283 pobj analysis,For
R4005 T6286 T6284 punct ", ",homogenized
R4006 T6287 T6284 nsubjpass tissue,homogenized
R4007 T6288 T6284 auxpass was,homogenized
R4008 T6289 T6284 cc and,homogenized
R4009 T6290 T6284 conj lysed,homogenized
R4010 T6291 T6290 prep in,lysed
R4011 T6292 T6293 compound RIPA,buffer
R4012 T6293 T6291 pobj buffer,in
R4013 T6294 T6295 punct (,PBS
R4014 T6295 T6293 parataxis PBS,buffer
R4015 T6296 T6295 nummod 1,PBS
R4016 T6297 T6296 punct ×,1
R4017 T6298 T6295 punct ", ",PBS
R4018 T6299 T6300 nummod 1,%
R4019 T6300 T6301 compound %,P
R4020 T6301 T6295 conj P,PBS
R4021 T6302 T6301 compound Nonidet,P
R4022 T6303 T6301 punct -,P
R4023 T6304 T6301 nummod 40,P
R4024 T6305 T6301 punct ", ",P
R4025 T6306 T6307 nummod 0.5,%
R4026 T6307 T6308 compound %,deoxycholate
R4027 T6308 T6301 conj deoxycholate,P
R4028 T6309 T6308 compound sodium,deoxycholate
R4029 T6310 T6308 punct ", ",deoxycholate
R4030 T6311 T6308 cc and,deoxycholate
R4031 T6312 T6313 nummod 1,%
R4032 T6313 T6314 compound %,SDS
R4033 T6314 T6308 conj SDS,deoxycholate
R4034 T6315 T6295 punct ),PBS
R4035 T6316 T6290 prep with,lysed
R4036 T6317 T6318 nummod 10,%
R4037 T6318 T6319 compound %,glycerol
R4038 T6319 T6316 pobj glycerol,with
R4039 T6320 T6319 cc and,glycerol
R4040 T6321 T6322 amod Complete,Inhibitor
R4041 T6322 T6319 conj Inhibitor,glycerol
R4042 T6323 T6322 compound Protease,Inhibitor
R4043 T6324 T6322 punct ", ",Inhibitor
R4044 T6325 T6326 punct (,Corporation
R4045 T6326 T6322 conj Corporation,Inhibitor
R4046 T6327 T6326 compound Roche,Corporation
R4047 T6328 T6326 compound Diagnostics,Corporation
R4048 T6329 T6326 punct ", ",Corporation
R4049 T6330 T6326 npadvmod Indianapolis,Corporation
R4050 T6331 T6326 punct ", ",Corporation
R4051 T6332 T6326 npadvmod Indiana,Corporation
R4052 T6333 T6326 punct ", ",Corporation
R4053 T6334 T6335 compound United,States
R4054 T6335 T6326 npadvmod States,Corporation
R4055 T6336 T6326 punct ),Corporation
R4056 T6337 T6319 punct ", ",glycerol
R4057 T6338 T6339 nummod 1,mM
R4058 T6339 T6319 conj mM,glycerol
R4059 T6340 T6339 prep of,mM
R4060 T6341 T6340 pobj phenylmethylsulfonylfluoride,of
R4061 T6342 T6339 punct ", ",mM
R4062 T6343 T6339 cc and,mM
R4063 T6344 T6345 nummod 1,mM
R4064 T6345 T6339 conj mM,mM
R4065 T6346 T6345 prep of,mM
R4066 T6347 T6348 compound sodium,orthovanadate
R4067 T6348 T6346 pobj orthovanadate,of
R4068 T6349 T6284 punct .,homogenized
R4069 T6351 T6352 nsubjpass Lysates,quantified
R4070 T6353 T6352 auxpass were,quantified
R4071 T6354 T6352 prep by,quantified
R4072 T6355 T6356 compound Bradford,assay
R4073 T6356 T6354 pobj assay,by
R4074 T6357 T6356 compound BCA,assay
R4075 T6358 T6356 compound protein,assay
R4076 T6359 T6360 punct (,Rad
R4077 T6360 T6356 parataxis Rad,assay
R4078 T6361 T6360 compound Bio,Rad
R4079 T6362 T6360 punct -,Rad
R4080 T6363 T6360 punct ", ",Rad
R4081 T6364 T6360 npadvmod Hercules,Rad
R4082 T6365 T6360 punct ", ",Rad
R4083 T6366 T6360 npadvmod California,Rad
R4084 T6367 T6360 punct ", ",Rad
R4085 T6368 T6369 compound United,States
R4086 T6369 T6360 npadvmod States,Rad
R4087 T6370 T6360 punct ),Rad
R4088 T6371 T6352 punct .,quantified
R4089 T6373 T6374 compound Protein,lysates
R4090 T6374 T6375 nsubjpass lysates,denatured
R4091 T6376 T6375 auxpass were,denatured
R4092 T6377 T6375 punct ", ",denatured
R4093 T6378 T6375 conj separated,denatured
R4094 T6379 T6378 prep in,separated
R4095 T6380 T6381 nummod 8,%
R4096 T6381 T6382 compound %,PAGE
R4097 T6382 T6379 pobj PAGE,in
R4098 T6383 T6382 compound SDS,PAGE
R4099 T6384 T6382 punct -,PAGE
R4100 T6385 T6378 punct ", ",separated
R4101 T6386 T6378 cc and,separated
R4102 T6387 T6378 conj transferred,separated
R4103 T6388 T6387 advmod overnight,transferred
R4104 T6389 T6387 prep onto,transferred
R4105 T6390 T6391 det a,membrane
R4106 T6391 T6389 pobj membrane,onto
R4107 T6392 T6393 nummod 0.45,μm
R4108 T6393 T6391 compound μm,membrane
R4109 T6394 T6391 compound nitrocellulose,membrane
R4110 T6395 T6396 punct (,Schleicher
R4111 T6396 T6391 parataxis Schleicher,membrane
R4112 T6397 T6396 cc and,Schleicher
R4113 T6398 T6396 conj Schuell,Schleicher
R4114 T6399 T6396 punct ", ",Schleicher
R4115 T6400 T6396 npadvmod Keene,Schleicher
R4116 T6401 T6396 punct ", ",Schleicher
R4117 T6402 T6403 compound New,Hampshire
R4118 T6403 T6396 npadvmod Hampshire,Schleicher
R4119 T6404 T6396 punct ", ",Schleicher
R4120 T6405 T6406 compound United,States
R4121 T6406 T6396 npadvmod States,Schleicher
R4122 T6407 T6396 punct ),Schleicher
R4123 T6408 T6375 punct .,denatured
R4124 T6410 T6411 prep After,immunoblotted
R4125 T6412 T6410 pcomp blocking,After
R4126 T6413 T6412 prep with,blocking
R4127 T6414 T6415 nummod 5,%
R4128 T6415 T6416 nmod %,milk
R4129 T6416 T6413 pobj milk,with
R4130 T6417 T6416 amod nonfat,milk
R4131 T6418 T6412 prep in,blocking
R4132 T6419 T6420 npadvmod phosphate,buffered
R4133 T6420 T6422 amod buffered,saline
R4134 T6421 T6420 punct -,buffered
R4135 T6422 T6418 pobj saline,in
R4136 T6423 T6422 prep with,saline
R4137 T6424 T6425 nummod 0.1,%
R4138 T6425 T6426 compound %,Tween
R4139 T6426 T6423 pobj Tween,with
R4140 T6427 T6426 punct -,Tween
R4141 T6428 T6426 nummod 20,Tween
R4142 T6429 T6411 punct ", ",immunoblotted
R4143 T6430 T6431 det the,membrane
R4144 T6431 T6411 nsubjpass membrane,immunoblotted
R4145 T6432 T6411 auxpass was,immunoblotted
R4146 T6433 T6411 advmod overnight,immunoblotted
R4147 T6434 T6411 prep at,immunoblotted
R4148 T6435 T6436 nummod 4,°C
R4149 T6436 T6434 pobj °C,at
R4150 T6437 T6411 prep with,immunoblotted
R4151 T6438 T6439 quantmod 1,500
R4152 T6439 T6441 nummod 500,dilution
R4153 T6440 T6439 punct :,500
R4154 T6441 T6437 pobj dilution,with
R4155 T6442 T6441 prep of,dilution
R4156 T6443 T6444 det an,antibody
R4157 T6444 T6442 pobj antibody,of
R4158 T6445 T6444 amod anti-MCAD,antibody
R4159 T6446 T6411 punct .,immunoblotted
R4160 T6448 T6449 nsubjpass Blots,incubated
R4161 T6450 T6449 auxpass were,incubated
R4162 T6451 T6449 prep in,incubated
R4163 T6452 T6453 amod anti-mouse,antibody
R4164 T6453 T6451 pobj antibody,in
R4165 T6454 T6453 nmod IgG,antibody
R4166 T6455 T6456 npadvmod HRP,conjugated
R4167 T6456 T6453 amod conjugated,antibody
R4168 T6457 T6456 punct -,conjugated
R4169 T6458 T6453 amod secondary,antibody
R4170 T6459 T6449 prep for,incubated
R4171 T6460 T6461 quantmod 2,4
R4172 T6461 T6463 nummod 4,h
R4173 T6462 T6461 punct –,4
R4174 T6463 T6459 pobj h,for
R4175 T6464 T6449 prep at,incubated
R4176 T6465 T6466 compound room,temperature
R4177 T6466 T6464 pobj temperature,at
R4178 T6467 T6449 advcl using,incubated
R4179 T6468 T6469 amod standard,procedures
R4180 T6469 T6467 dobj procedures,using
R4181 T6470 T6449 cc and,incubated
R4182 T6471 T6449 conj developed,incubated
R4183 T6472 T6471 prep by,developed
R4184 T6473 T6472 pobj chemiluminiscence,by
R4185 T6474 T6475 punct (,Renaissance
R4186 T6475 T6473 parataxis Renaissance,chemiluminiscence
R4187 T6476 T6475 punct ", ",Renaissance
R4188 T6477 T6478 compound NEN,Products
R4189 T6478 T6475 appos Products,Renaissance
R4190 T6479 T6478 compound Lifesciences,Products
R4191 T6480 T6475 punct ", ",Renaissance
R4192 T6481 T6475 npadvmod Boston,Renaissance
R4193 T6482 T6475 punct ", ",Renaissance
R4194 T6483 T6475 npadvmod Massachusetts,Renaissance
R4195 T6484 T6475 punct ", ",Renaissance
R4196 T6485 T6486 compound United,States
R4197 T6486 T6475 npadvmod States,Renaissance
R4198 T6487 T6475 punct ),Renaissance
R4199 T6488 T6449 punct .,incubated
R4200 T6565 T6566 compound Liver,enzyme
R4201 T6566 T6567 compound enzyme,activity
R4202 T6568 T6567 punct .,activity
R4203 T6570 T6571 prep In,prepared
R4204 T6572 T6570 pobj order,In
R4205 T6573 T6574 aux to,evaluate
R4206 T6574 T6572 acl evaluate,order
R4207 T6575 T6576 compound MCAD,activity
R4208 T6576 T6574 dobj activity,evaluate
R4209 T6577 T6574 prep in,evaluate
R4210 T6578 T6577 pobj mice,in
R4211 T6579 T6571 punct ", ",prepared
R4212 T6580 T6581 compound liver,homogenates
R4213 T6581 T6571 nsubjpass homogenates,prepared
R4214 T6582 T6571 auxpass were,prepared
R4215 T6583 T6571 prep from,prepared
R4216 T6584 T6585 nmod MCAD,mice
R4217 T6585 T6583 pobj mice,from
R4218 T6586 T6584 punct +,MCAD
R4219 T6587 T6584 punct /,MCAD
R4220 T6588 T6584 punct +,MCAD
R4221 T6589 T6590 punct (,5
R4222 T6590 T6584 parataxis 5,MCAD
R4223 T6591 T6590 nsubj n,5
R4224 T6592 T6590 punct =,5
R4225 T6593 T6590 punct ),5
R4226 T6594 T6584 cc and,MCAD
R4227 T6595 T6584 conj MCAD,MCAD
R4228 T6596 T6595 punct −,MCAD
R4229 T6597 T6595 punct /,MCAD
R4230 T6598 T6595 punct −,MCAD
R4231 T6599 T6600 punct (,5
R4232 T6600 T6585 parataxis 5,mice
R4233 T6601 T6600 nsubj n,5
R4234 T6602 T6600 punct =,5
R4235 T6603 T6600 punct ),5
R4236 T6604 T6571 punct .,prepared
R4237 T6606 T6607 det The,assay
R4238 T6607 T6615 nsubjpass assay,used
R4239 T6608 T6607 amod sensitive,assay
R4240 T6609 T6608 cc and,sensitive
R4241 T6610 T6611 advmod highly,specific
R4242 T6611 T6608 conj specific,sensitive
R4243 T6612 T6613 amod anaerobic,ETF
R4244 T6613 T6607 compound ETF,assay
R4245 T6614 T6607 compound reduction,assay
R4246 T6616 T6615 auxpass was,used
R4247 T6617 T6615 prep on,used
R4248 T6618 T6619 compound tissue,extracts
R4249 T6619 T6617 pobj extracts,on
R4250 T6620 T6619 prep with,extracts
R4251 T6621 T6622 compound octanoyl,CoA
R4252 T6622 T6620 pobj CoA,with
R4253 T6623 T6622 punct -,CoA
R4254 T6624 T6625 punct (,C8
R4255 T6625 T6622 parataxis C8,CoA
R4256 T6626 T6625 punct ),C8
R4257 T6627 T6622 cc and,CoA
R4258 T6628 T6629 compound palmitoyl,CoA
R4259 T6629 T6622 conj CoA,CoA
R4260 T6630 T6629 punct -,CoA
R4261 T6631 T6632 punct (,C16
R4262 T6632 T6629 parataxis C16,CoA
R4263 T6633 T6632 punct ),C16
R4264 T6634 T6622 prep as,CoA
R4265 T6635 T6634 pobj substrate,as
R4266 T6636 T6637 mark as,described
R4267 T6637 T6615 advcl described,used
R4268 T6638 T6637 advmod previously,described
R4269 T6639 T6640 punct [,29
R4270 T6640 T6615 parataxis 29,used
R4271 T6641 T6640 punct ],29
R4272 T6642 T6615 punct .,used
R4273 T6683 T6682 cc and,Fasting
R4274 T6684 T6685 compound cold,challenge
R4275 T6685 T6682 conj challenge,Fasting
R4276 T6686 T6685 punct .,challenge
R4277 T6688 T6689 nummod Eight,wk
R4278 T6689 T6691 npadvmod wk,old
R4279 T6690 T6689 punct -,wk
R4280 T6691 T6693 amod old,mice
R4281 T6692 T6691 punct -,old
R4282 T6693 T6703 nsubjpass mice,fasted
R4283 T6694 T6693 nmod MCAD,mice
R4284 T6695 T6694 punct +,MCAD
R4285 T6696 T6694 punct /,MCAD
R4286 T6697 T6694 punct +,MCAD
R4287 T6698 T6694 cc and,MCAD
R4288 T6699 T6694 conj MCAD,MCAD
R4289 T6700 T6699 punct −,MCAD
R4290 T6701 T6699 punct /,MCAD
R4291 T6702 T6699 punct −,MCAD
R4292 T6704 T6703 auxpass were,fasted
R4293 T6705 T6703 prep for,fasted
R4294 T6706 T6707 nummod 18,h
R4295 T6707 T6705 pobj h,for
R4296 T6708 T6709 punct (,experiments
R4297 T6709 T6707 parataxis experiments,h
R4298 T6710 T6711 compound cold,tolerance
R4299 T6711 T6709 compound tolerance,experiments
R4300 T6712 T6709 punct ),experiments
R4301 T6713 T6707 cc or,h
R4302 T6714 T6715 nummod 24,h
R4303 T6715 T6707 conj h,h
R4304 T6716 T6717 punct (,experiments
R4305 T6717 T6715 parataxis experiments,h
R4306 T6718 T6719 nmod serum,glucose
R4307 T6719 T6717 nmod glucose,experiments
R4308 T6720 T6719 punct ", ",glucose
R4309 T6721 T6722 amod free,acid
R4310 T6722 T6719 conj acid,glucose
R4311 T6723 T6722 amod fatty,acid
R4312 T6724 T6722 punct ", ",acid
R4313 T6725 T6726 amod organic,acid
R4314 T6726 T6722 conj acid,acid
R4315 T6727 T6726 punct ", ",acid
R4316 T6728 T6726 cc and,acid
R4317 T6729 T6726 conj carnitine,acid
R4318 T6730 T6717 punct ),experiments
R4319 T6731 T6732 amod prior,to
R4320 T6732 T6703 prep to,fasted
R4321 T6733 T6732 pobj analysis,to
R4322 T6734 T6703 punct .,fasted
R4323 T6736 T6737 compound Glucose,concentration
R4324 T6737 T6738 nsubjpass concentration,measured
R4325 T6739 T6738 auxpass was,measured
R4326 T6740 T6738 prep in,measured
R4327 T6741 T6742 nummod 10,μl
R4328 T6742 T6743 compound μl,sera
R4329 T6743 T6740 pobj sera,in
R4330 T6744 T6738 advcl using,measured
R4331 T6745 T6746 det an,system
R4332 T6746 T6744 dobj system,using
R4333 T6747 T6748 nmod Ektachem,DT
R4334 T6748 T6746 nmod DT,system
R4335 T6749 T6748 nummod II,DT
R4336 T6750 T6751 punct (,Diagnostics
R4337 T6751 T6746 parataxis Diagnostics,system
R4338 T6752 T6751 nmod Johnson,Diagnostics
R4339 T6753 T6752 cc and,Johnson
R4340 T6754 T6752 conj Johnson,Johnson
R4341 T6755 T6751 compound Clinical,Diagnostics
R4342 T6756 T6751 punct ", ",Diagnostics
R4343 T6757 T6751 npadvmod Rochester,Diagnostics
R4344 T6758 T6751 punct ", ",Diagnostics
R4345 T6759 T6760 compound New,York
R4346 T6760 T6751 npadvmod York,Diagnostics
R4347 T6761 T6751 punct ", ",Diagnostics
R4348 T6762 T6763 compound United,States
R4349 T6763 T6751 npadvmod States,Diagnostics
R4350 T6764 T6751 punct ),Diagnostics
R4351 T6765 T6738 punct .,measured
R4352 T6767 T6768 amod Total,acids
R4353 T6768 T6771 nsubjpass acids,measured
R4354 T6769 T6768 amod non-esterified,acids
R4355 T6770 T6768 amod fatty,acids
R4356 T6772 T6768 punct (,acids
R4357 T6773 T6768 appos NEFA,acids
R4358 T6774 T6771 punct ),measured
R4359 T6775 T6771 auxpass were,measured
R4360 T6776 T6771 prep by,measured
R4361 T6777 T6778 det an,method
R4362 T6778 T6776 pobj method,by
R4363 T6779 T6778 amod enzymatic,method
R4364 T6780 T6778 punct ", ",method
R4365 T6781 T6778 amod colorimetric,method
R4366 T6782 T6783 punct (,Diagnostics
R4367 T6783 T6771 parataxis Diagnostics,measured
R4368 T6784 T6783 punct “,Diagnostics
R4369 T6785 T6786 nmod NEFA,C
R4370 T6786 T6788 nmod C,reagents
R4371 T6787 T6786 punct -,C
R4372 T6788 T6783 dep reagents,Diagnostics
R4373 T6789 T6788 punct ”,reagents
R4374 T6790 T6783 punct ", ",Diagnostics
R4375 T6791 T6783 compound Wako,Diagnostics
R4376 T6792 T6783 punct ", ",Diagnostics
R4377 T6793 T6783 npadvmod Richmond,Diagnostics
R4378 T6794 T6783 punct ", ",Diagnostics
R4379 T6795 T6783 npadvmod Virginia,Diagnostics
R4380 T6796 T6783 punct ", ",Diagnostics
R4381 T6797 T6798 compound United,States
R4382 T6798 T6783 npadvmod States,Diagnostics
R4383 T6799 T6783 punct ),Diagnostics
R4384 T6800 T6771 punct .,measured
R4385 T6802 T6803 det The,assay
R4386 T6803 T6804 nsubjpass assay,modified
R4387 T6805 T6804 auxpass was,modified
R4388 T6806 T6807 aux to,accommodate
R4389 T6807 T6804 advcl accommodate,modified
R4390 T6808 T6809 det a,size
R4391 T6809 T6807 dobj size,accommodate
R4392 T6810 T6809 amod reduced,size
R4393 T6811 T6809 compound sample,size
R4394 T6812 T6813 punct (,μl
R4395 T6813 T6809 parataxis μl,size
R4396 T6814 T6813 nummod 10,μl
R4397 T6815 T6813 punct ),μl
R4398 T6816 T6809 cc and,size
R4399 T6817 T6809 conj use,size
R4400 T6818 T6817 prep of,use
R4401 T6819 T6820 det a,reader
R4402 T6820 T6818 pobj reader,of
R4403 T6821 T6820 compound microplate,reader
R4404 T6822 T6820 prep for,reader
R4405 T6823 T6822 pobj measurement,for
R4406 T6824 T6823 prep of,measurement
R4407 T6825 T6826 amod optical,density
R4408 T6826 T6824 pobj density,of
R4409 T6827 T6826 prep at,density
R4410 T6828 T6829 nummod 550,nm
R4411 T6829 T6827 pobj nm,at
R4412 T6830 T6804 punct .,modified
R4413 T6832 T6833 nmod Urine,acid
R4414 T6833 T6835 compound acid,analyses
R4415 T6834 T6833 amod organic,acid
R4416 T6835 T6836 nsubjpass analyses,performed
R4417 T6837 T6836 auxpass were,performed
R4418 T6838 T6836 advcl using,performed
R4419 T6839 T6840 compound gas,chromatography
R4420 T6840 T6838 dobj chromatography,using
R4421 T6841 T6840 punct -,chromatography
R4422 T6842 T6843 compound mass,spectroscopy
R4423 T6843 T6840 appos spectroscopy,chromatography
R4424 T6844 T6845 mark as,described
R4425 T6845 T6838 advcl described,using
R4426 T6846 T6845 advmod previously,described
R4427 T6847 T6848 punct [,30
R4428 T6848 T6836 parataxis 30,performed
R4429 T6849 T6848 nummod 22,30
R4430 T6850 T6848 punct ",",30
R4431 T6851 T6848 punct ],30
R4432 T6852 T6836 punct ", ",performed
R4433 T6853 T6836 prep except,performed
R4434 T6854 T6855 nsubjpass tetracosane,used
R4435 T6855 T6853 pcomp used,except
R4436 T6856 T6857 punct (,Sigma
R4437 T6857 T6854 parataxis Sigma,tetracosane
R4438 T6858 T6857 dep C24,Sigma
R4439 T6859 T6857 punct ;,Sigma
R4440 T6860 T6857 punct ", ",Sigma
R4441 T6861 T6862 compound St.,Louis
R4442 T6862 T6857 npadvmod Louis,Sigma
R4443 T6863 T6857 punct ", ",Sigma
R4444 T6864 T6857 npadvmod Missouri,Sigma
R4445 T6865 T6857 punct ", ",Sigma
R4446 T6866 T6867 compound United,States
R4447 T6867 T6857 npadvmod States,Sigma
R4448 T6868 T6857 punct ),Sigma
R4449 T6869 T6855 auxpass was,used
R4450 T6870 T6855 prep as,used
R4451 T6871 T6872 det the,standard
R4452 T6872 T6870 pobj standard,as
R4453 T6873 T6872 amod internal,standard
R4454 T6874 T6836 punct ", ",performed
R4455 T6875 T6836 cc and,performed
R4456 T6876 T6877 amod quantitative,determinations
R4457 T6877 T6878 nsubjpass determinations,made
R4458 T6878 T6836 conj made,performed
R4459 T6879 T6878 auxpass were,made
R4460 T6880 T6878 prep based,made
R4461 T6881 T6880 prep on,based
R4462 T6882 T6881 pobj comparison,on
R4463 T6883 T6882 prep with,comparison
R4464 T6884 T6885 amod synthetic,standards
R4465 T6885 T6883 pobj standards,with
R4466 T6886 T6885 compound calibration,standards
R4467 T6887 T6878 punct .,made
R4468 T6889 T6890 compound Acylcarnitine,analyses
R4469 T6890 T6891 nsubjpass analyses,conducted
R4470 T6892 T6890 prep in,analyses
R4471 T6893 T6892 pobj serum,in
R4472 T6894 T6893 cc and,serum
R4473 T6895 T6893 conj bile,serum
R4474 T6896 T6891 auxpass were,conducted
R4475 T6897 T6891 advcl using,conducted
R4476 T6898 T6899 nmod electrospray,spectrometry
R4477 T6899 T6897 dobj spectrometry,using
R4478 T6900 T6899 amod tandem,spectrometry
R4479 T6901 T6899 compound mass,spectrometry
R4480 T6902 T6903 punct [,32
R4481 T6903 T6891 parataxis 32,conducted
R4482 T6904 T6903 nummod 31,32
R4483 T6905 T6903 punct ",",32
R4484 T6906 T6903 punct ],32
R4485 T6907 T6891 punct .,conducted
R4486 T6958 T6957 punct .,Histopathology
R4487 T6960 T6961 nummod Twelve,mice
R4488 T6961 T6962 nsubjpass mice,examined
R4489 T6963 T6962 auxpass were,examined
R4490 T6964 T6962 prep for,examined
R4491 T6965 T6966 amod gross,abnormalities
R4492 T6966 T6964 pobj abnormalities,for
R4493 T6967 T6965 cc and,gross
R4494 T6968 T6965 conj histologic,gross
R4495 T6969 T6962 punct ", ",examined
R4496 T6970 T6962 prep including,examined
R4497 T6971 T6970 pobj one,including
R4498 T6972 T6971 amod male,one
R4499 T6973 T6971 cc and,one
R4500 T6974 T6975 nummod one,mouse
R4501 T6975 T6971 conj mouse,one
R4502 T6976 T6974 amod female,one
R4503 T6977 T6975 nmod MCAD,mouse
R4504 T6978 T6977 punct −,MCAD
R4505 T6979 T6977 punct /,MCAD
R4506 T6980 T6977 punct −,MCAD
R4507 T6981 T6982 nummod 18,mo
R4508 T6982 T6984 npadvmod mo,old
R4509 T6983 T6982 punct -,mo
R4510 T6984 T6971 amod old,one
R4511 T6985 T6984 punct -,old
R4512 T6986 T6971 punct ", ",one
R4513 T6987 T6971 conj one,one
R4514 T6988 T6987 amod male,one
R4515 T6989 T6987 cc and,one
R4516 T6990 T6991 nummod one,mouse
R4517 T6991 T6987 conj mouse,one
R4518 T6992 T6990 amod female,one
R4519 T6993 T6991 nmod MCAD,mouse
R4520 T6994 T6993 punct +,MCAD
R4521 T6995 T6993 punct /,MCAD
R4522 T6996 T6993 punct +,MCAD
R4523 T6997 T6998 nummod 6,mo
R4524 T6998 T7000 npadvmod mo,old
R4525 T6999 T6998 punct -,mo
R4526 T7000 T6987 amod old,one
R4527 T7001 T7000 punct -,old
R4528 T7002 T6987 punct ", ",one
R4529 T7003 T6987 conj two,one
R4530 T7004 T7003 amod male,two
R4531 T7005 T7003 cc and,two
R4532 T7006 T7007 nummod two,mice
R4533 T7007 T7003 conj mice,two
R4534 T7008 T7006 amod female,two
R4535 T7009 T7007 nmod MCAD,mice
R4536 T7010 T7009 punct −,MCAD
R4537 T7011 T7009 punct /,MCAD
R4538 T7012 T7009 punct −,MCAD
R4539 T7013 T7014 nummod 4,wk
R4540 T7014 T7016 npadvmod wk,old
R4541 T7015 T7014 punct -,wk
R4542 T7016 T7003 amod old,two
R4543 T7017 T7016 punct -,old
R4544 T7018 T7003 punct ", ",two
R4545 T7019 T7003 cc and,two
R4546 T7020 T7003 conj two,two
R4547 T7021 T7020 amod male,two
R4548 T7022 T7020 cc and,two
R4549 T7023 T7024 nummod two,mice
R4550 T7024 T7020 conj mice,two
R4551 T7025 T7023 amod female,two
R4552 T7026 T7024 nmod MCAD,mice
R4553 T7027 T7026 punct +,MCAD
R4554 T7028 T7026 punct /,MCAD
R4555 T7029 T7026 punct +,MCAD
R4556 T7030 T7031 nummod 4,wk
R4557 T7031 T7033 npadvmod wk,old
R4558 T7032 T7031 punct -,wk
R4559 T7033 T7020 amod old,two
R4560 T7034 T7033 punct -,old
R4561 T7035 T6962 punct .,examined
R4562 T7037 T7038 nsubjpass Kidney,fixed
R4563 T7039 T7037 punct ", ",Kidney
R4564 T7040 T7037 conj spleen,Kidney
R4565 T7041 T7040 punct ", ",spleen
R4566 T7042 T7040 conj pancreas,spleen
R4567 T7043 T7042 punct ", ",pancreas
R4568 T7044 T7042 conj liver,pancreas
R4569 T7045 T7044 punct ", ",liver
R4570 T7046 T7044 conj brain,liver
R4571 T7047 T7046 punct ", ",brain
R4572 T7048 T7046 conj heart,brain
R4573 T7049 T7048 punct ", ",heart
R4574 T7050 T7048 conj testicles,heart
R4575 T7051 T7050 punct ", ",testicles
R4576 T7052 T7050 conj ovaries,testicles
R4577 T7053 T7052 punct ", ",ovaries
R4578 T7054 T7052 cc and,ovaries
R4579 T7055 T7056 amod skeletal,muscle
R4580 T7056 T7052 conj muscle,ovaries
R4581 T7057 T7038 auxpass were,fixed
R4582 T7058 T7038 prep by,fixed
R4583 T7059 T7058 pobj immersion,by
R4584 T7060 T7059 prep in,immersion
R4585 T7061 T7062 amod buffered,formalin
R4586 T7062 T7060 pobj formalin,in
R4587 T7063 T7064 nummod 10,%
R4588 T7064 T7062 compound %,formalin
R4589 T7065 T7038 punct ", ",fixed
R4590 T7066 T7038 conj processed,fixed
R4591 T7067 T7066 advmod routinely,processed
R4592 T7068 T7066 prep for,processed
R4593 T7069 T7070 compound paraffin,sectioning
R4594 T7070 T7068 pobj sectioning,for
R4595 T7071 T7066 punct ", ",processed
R4596 T7072 T7066 conj sectioned,processed
R4597 T7073 T7072 prep at,sectioned
R4598 T7074 T7075 nummod 5,μm
R4599 T7075 T7073 pobj μm,at
R4600 T7076 T7072 punct ", ",sectioned
R4601 T7077 T7072 cc and,sectioned
R4602 T7078 T7072 conj stained,sectioned
R4603 T7079 T7078 prep with,stained
R4604 T7080 T7079 pobj hematoxylin,with
R4605 T7081 T7080 cc and,hematoxylin
R4606 T7082 T7080 conj eosin,hematoxylin
R4607 T7083 T7038 punct .,fixed
R4608 T7085 T7086 amod Frozen,sections
R4609 T7086 T7088 nsubjpass sections,prepared
R4610 T7087 T7086 compound liver,sections
R4611 T7089 T7088 auxpass were,prepared
R4612 T7090 T7088 advcl using,prepared
R4613 T7091 T7092 amod standard,methods
R4614 T7092 T7090 dobj methods,using
R4615 T7093 T7088 cc and,prepared
R4616 T7094 T7095 nsubjpass sections,stained
R4617 T7095 T7088 conj stained,prepared
R4618 T7096 T7095 auxpass were,stained
R4619 T7097 T7095 prep with,stained
R4620 T7098 T7099 nmod Oil,O
R4621 T7099 T7097 pobj O,with
R4622 T7100 T7099 punct -,O
R4623 T7101 T7099 amod red,O
R4624 T7102 T7099 punct -,O
R4625 T7103 T7095 punct .,stained
R4626 T7105 T7106 nsubjpass Slides,examined
R4627 T7107 T7106 auxpass were,examined
R4628 T7108 T7106 prep without,examined
R4629 T7109 T7108 pobj knowledge,without
R4630 T7110 T7109 prep of,knowledge
R4631 T7111 T7110 pobj genotype,of
R4632 T7112 T7111 cc or,genotype
R4633 T7113 T7111 conj age,genotype
R4634 T7114 T7106 punct .,examined
R4635 T7116 T7117 amod Other,mice
R4636 T7117 T7118 nsubjpass mice,examined
R4637 T7119 T7118 auxpass were,examined
R4638 T7120 T7118 prep because,examined
R4639 T7121 T7120 pcomp of,because
R4640 T7122 T7123 amod sporadic,disease
R4641 T7123 T7120 pobj disease,because
R4642 T7124 T7123 amod clinical,disease
R4643 T7125 T7118 punct .,examined
R4644 T7127 T7128 nsubjpass Those,evaluated
R4645 T7129 T7127 prep with,Those
R4646 T7130 T7131 amod visible,enlargement
R4647 T7131 T7129 pobj enlargement,with
R4648 T7132 T7131 amod cardiac,enlargement
R4649 T7133 T7128 auxpass were,evaluated
R4650 T7134 T7128 prep for,evaluated
R4651 T7135 T7136 amod cardiac,lesions
R4652 T7136 T7134 pobj lesions,for
R4653 T7137 T7128 punct .,evaluated
R4654 T7201 T7202 amod Statistical,analyses
R4655 T7203 T7202 punct .,analyses
R4656 T7205 T7206 nsubjpass Results,tested
R4657 T7207 T7205 prep between,Results
R4658 T7208 T7207 pobj groups,between
R4659 T7209 T7206 auxpass were,tested
R4660 T7210 T7206 prep for,tested
R4661 T7211 T7212 amod statistical,significance
R4662 T7212 T7210 pobj significance,for
R4663 T7213 T7206 advcl using,tested
R4664 T7214 T7215 poss Student,test
R4665 T7215 T7213 dobj test,using
R4666 T7216 T7214 case 's,Student
R4667 T7217 T7215 compound t,test
R4668 T7218 T7215 punct -,test
R4669 T7219 T7206 punct .,tested
R4670 T7221 T7222 det A,p
R4671 T7222 T7223 nsubjpass p,set
R4672 T7224 T7225 punct <,0.05
R4673 T7225 T7222 amod 0.05,p
R4674 T7226 T7223 auxpass was,set
R4675 T7227 T7223 prep as,set
R4676 T7228 T7227 amod significant,as
R4677 T7229 T7223 punct .,set
R4678 T7248 T7247 prep for,Strategy
R4679 T7249 T7248 pobj Disruption,for
R4680 T7250 T7249 prep of,Disruption
R4681 T7251 T7252 det the,Gene
R4682 T7252 T7250 pobj Gene,of
R4683 T7253 T7252 compound Mouse,Gene
R4684 T7254 T7252 compound Acadm,Gene
R4685 T7256 T7257 punct (,A
R4686 T7257 T7258 meta A,vector
R4687 T7259 T7257 punct ),A
R4688 T7260 T7258 det The,vector
R4689 T7261 T7262 compound MCAD,insertion
R4690 T7262 T7258 compound insertion,vector
R4691 T7263 T7262 compound IV2,insertion
R4692 T7264 T7258 compound targeting,vector
R4693 T7265 T7258 prep with,vector
R4694 T7266 T7267 det a,region
R4695 T7267 T7265 pobj region,with
R4696 T7268 T7267 amod deleted,region
R4697 T7269 T7270 nummod 1.3,kb
R4698 T7270 T7267 compound kb,region
R4699 T7271 T7270 punct -,kb
R4700 T7272 T7267 acl encompassing,region
R4701 T7273 T7272 dobj exon,encompassing
R4702 T7274 T7273 nummod 10,exon
R4703 T7275 T7273 cc and,exon
R4704 T7276 T7277 amod flanking,sequences
R4705 T7277 T7273 conj sequences,exon
R4706 T7278 T7258 punct .,vector
R4707 T7280 T7281 compound MCAD,IV2
R4708 T7281 T7282 nsubj IV2,undergoes
R4709 T7283 T7284 compound gap,repair
R4710 T7284 T7282 dobj repair,undergoes
R4711 T7285 T7282 prep upon,undergoes
R4712 T7286 T7287 amod homologous,recombination
R4713 T7287 T7285 pobj recombination,upon
R4714 T7288 T7282 prep at,undergoes
R4715 T7289 T7290 det the,locus
R4716 T7290 T7288 pobj locus,at
R4717 T7291 T7290 amod endogenous,locus
R4718 T7292 T7290 compound Acadm,locus
R4719 T7293 T7282 advcl resulting,undergoes
R4720 T7294 T7293 prep in,resulting
R4721 T7295 T7296 det a,duplication
R4722 T7296 T7294 pobj duplication,in
R4723 T7297 T7296 prep of,duplication
R4724 T7298 T7299 nmod exons,8
R4725 T7299 T7297 pobj 8,of
R4726 T7300 T7299 punct ", ",8
R4727 T7301 T7299 conj 9,8
R4728 T7302 T7301 punct ", ",9
R4729 T7303 T7301 cc and,9
R4730 T7304 T7301 conj 10,9
R4731 T7305 T7296 prep at,duplication
R4732 T7306 T7307 det the,allele
R4733 T7307 T7305 pobj allele,at
R4734 T7308 T7307 amod disrupted,allele
R4735 T7309 T7282 punct .,undergoes
R4736 T7311 T7312 punct (,B
R4737 T7312 T7313 meta B,analysis
R4738 T7314 T7312 punct ),B
R4739 T7315 T7316 compound Southern,blot
R4740 T7316 T7313 compound blot,analysis
R4741 T7317 T7313 prep of,analysis
R4742 T7318 T7319 npadvmod EcoRI,digested
R4743 T7319 T7321 amod digested,DNA
R4744 T7320 T7319 punct -,digested
R4745 T7321 T7317 pobj DNA,of
R4746 T7322 T7321 amod genomic,DNA
R4747 T7323 T7321 prep from,DNA
R4748 T7324 T7325 compound ES,cells
R4749 T7325 T7323 pobj cells,from
R4750 T7326 T7325 acl screened,cells
R4751 T7327 T7326 prep by,screened
R4752 T7328 T7327 pobj PCR,by
R4753 T7329 T7313 punct .,analysis
R4754 T7331 T7332 compound Probe,A
R4755 T7332 T7333 nsubj A,hybridizes
R4756 T7334 T7332 punct ", ",A
R4757 T7335 T7336 det a,fragment
R4758 T7336 T7332 appos fragment,A
R4759 T7337 T7336 compound DNA,fragment
R4760 T7338 T7336 acl consisting,fragment
R4761 T7339 T7338 prep of,consisting
R4762 T7340 T7341 det a,portion
R4763 T7341 T7339 pobj portion,of
R4764 T7342 T7341 prep of,portion
R4765 T7343 T7342 pobj exon,of
R4766 T7344 T7343 nummod 10,exon
R4767 T7345 T7346 dep that,is
R4768 T7346 T7341 relcl is,portion
R4769 T7347 T7346 neg not,is
R4770 T7348 T7346 acomp present,is
R4771 T7349 T7346 prep in,is
R4772 T7350 T7351 det the,vector
R4773 T7351 T7349 pobj vector,in
R4774 T7352 T7351 compound targeting,vector
R4775 T7353 T7333 punct ", ",hybridizes
R4776 T7354 T7333 prep to,hybridizes
R4777 T7355 T7356 det an,fragment
R4778 T7356 T7354 pobj fragment,to
R4779 T7357 T7356 amod endogenous,fragment
R4780 T7358 T7359 nummod 3.1,kb
R4781 T7359 T7356 compound kb,fragment
R4782 T7360 T7359 punct -,kb
R4783 T7361 T7333 cc and,hybridizes
R4784 T7362 T7333 punct ", ",hybridizes
R4785 T7363 T7364 prep upon,to
R4786 T7364 T7333 conj to,hybridizes
R4787 T7365 T7366 amod homologous,recombination
R4788 T7366 T7363 pobj recombination,upon
R4789 T7367 T7364 punct ", ",to
R4790 T7368 T7369 det a,fragment
R4791 T7369 T7364 pobj fragment,to
R4792 T7370 T7371 nummod 13.2,kb
R4793 T7371 T7369 compound kb,fragment
R4794 T7372 T7371 punct -,kb
R4795 T7373 T7333 punct .,hybridizes
R4796 T7375 T7376 nsubj Lane,represents
R4797 T7377 T7375 nummod 1,Lane
R4798 T7378 T7379 det a,line
R4799 T7379 T7376 dobj line,represents
R4800 T7380 T7381 amod wild,type
R4801 T7381 T7379 compound type,line
R4802 T7382 T7381 punct -,type
R4803 T7383 T7379 compound ES,line
R4804 T7384 T7379 compound cell,line
R4805 T7385 T7376 punct ", ",represents
R4806 T7386 T7376 cc and,represents
R4807 T7387 T7388 nmod Lane,2
R4808 T7388 T7389 nsubj 2,represent
R4809 T7389 T7376 conj represent,represents
R4810 T7390 T7388 cc and,2
R4811 T7391 T7388 conj 3,2
R4812 T7392 T7393 amod targeted,lines
R4813 T7393 T7389 dobj lines,represent
R4814 T7394 T7393 compound ES,lines
R4815 T7395 T7393 compound cell,lines
R4816 T7396 T7389 punct .,represent
R4817 T7455 T7456 compound Northern,Blot
R4818 T7456 T7457 compound Blot,Analysis
R4819 T7458 T7457 prep from,Analysis
R4820 T7459 T7460 nmod MCAD,Mice
R4821 T7460 T7458 pobj Mice,from
R4822 T7461 T7459 punct −,MCAD
R4823 T7462 T7459 punct /,MCAD
R4824 T7463 T7459 punct −,MCAD
R4825 T7464 T7465 punct (,2
R4826 T7465 T7459 parataxis 2,MCAD
R4827 T7466 T7465 nsubj n,2
R4828 T7467 T7465 punct =,2
R4829 T7468 T7465 punct ),2
R4830 T7469 T7459 cc and,MCAD
R4831 T7470 T7459 conj MCAD,MCAD
R4832 T7471 T7470 punct +,MCAD
R4833 T7472 T7470 punct /,MCAD
R4834 T7473 T7470 punct +,MCAD
R4835 T7474 T7475 punct (,2
R4836 T7475 T7470 parataxis 2,MCAD
R4837 T7476 T7475 nsubj n,2
R4838 T7477 T7475 punct =,2
R4839 T7478 T7475 punct ),2
R4840 T7480 T7481 compound Acadm,message
R4841 T7481 T7482 nsubjpass message,detected
R4842 T7483 T7482 auxpass was,detected
R4843 T7484 T7482 prep from,detected
R4844 T7485 T7486 det the,heart
R4845 T7486 T7484 pobj heart,from
R4846 T7487 T7486 punct ", ",heart
R4847 T7488 T7486 conj liver,heart
R4848 T7489 T7488 punct ", ",liver
R4849 T7490 T7491 amod brown,fat
R4850 T7491 T7488 conj fat,liver
R4851 T7492 T7491 punct ", ",fat
R4852 T7493 T7491 conj brain,fat
R4853 T7494 T7493 punct ", ",brain
R4854 T7495 T7493 conj kidney,brain
R4855 T7496 T7495 punct ", ",kidney
R4856 T7497 T7495 cc and,kidney
R4857 T7498 T7495 conj muscle,kidney
R4858 T7499 T7486 punct (,heart
R4859 T7500 T7486 cc and,heart
R4860 T7501 T7502 amod white,fat
R4861 T7502 T7486 conj fat,heart
R4862 T7503 T7502 cc and,fat
R4863 T7504 T7502 conj testes,fat
R4864 T7505 T7506 punct ", ",shown
R4865 T7506 T7486 parataxis shown,heart
R4866 T7507 T7506 nsubj data,shown
R4867 T7508 T7506 neg not,shown
R4868 T7509 T7506 punct ),shown
R4869 T7510 T7486 prep of,heart
R4870 T7511 T7512 amod only,mice
R4871 T7512 T7510 pobj mice,of
R4872 T7513 T7512 nmod MCAD,mice
R4873 T7514 T7513 punct +,MCAD
R4874 T7515 T7513 punct /,MCAD
R4875 T7516 T7513 punct +,MCAD
R4876 T7517 T7482 punct .,detected
R4877 T7519 T7520 advmod Most,expression
R4878 T7520 T7522 nsubj expression,occurred
R4879 T7521 T7520 amod robust,expression
R4880 T7523 T7522 prep in,occurred
R4881 T7524 T7525 amod brown,fat
R4882 T7525 T7523 pobj fat,in
R4883 T7526 T7525 punct ", ",fat
R4884 T7527 T7525 conj kidney,fat
R4885 T7528 T7527 punct ", ",kidney
R4886 T7529 T7527 conj heart,kidney
R4887 T7530 T7529 punct ", ",heart
R4888 T7531 T7529 cc and,heart
R4889 T7532 T7533 amod skeletal,muscle
R4890 T7533 T7529 conj muscle,heart
R4891 T7534 T7522 punct .,occurred
R4892 T7536 T7537 nmod MCAD,mice
R4893 T7537 T7541 nsubj mice,had
R4894 T7538 T7536 punct −,MCAD
R4895 T7539 T7536 punct /,MCAD
R4896 T7540 T7536 punct −,MCAD
R4897 T7542 T7543 det no,message
R4898 T7543 T7541 dobj message,had
R4899 T7544 T7543 amod detectable,message
R4900 T7545 T7541 prep in,had
R4901 T7546 T7547 det all,tissues
R4902 T7547 T7545 pobj tissues,in
R4903 T7548 T7547 acl examined,tissues
R4904 T7549 T7541 punct .,had
R4905 T7639 T7638 prep of,Immunoblots
R4906 T7640 T7641 compound Liver,Homogenates
R4907 T7641 T7639 pobj Homogenates,of
R4908 T7642 T7638 prep from,Immunoblots
R4909 T7643 T7644 nmod MCAD,Mice
R4910 T7644 T7642 pobj Mice,from
R4911 T7645 T7643 punct +,MCAD
R4912 T7646 T7643 punct /,MCAD
R4913 T7647 T7643 punct +,MCAD
R4914 T7648 T7643 cc and,MCAD
R4915 T7649 T7643 conj MCAD,MCAD
R4916 T7650 T7649 punct −,MCAD
R4917 T7651 T7649 punct /,MCAD
R4918 T7652 T7649 punct −,MCAD
R4919 T7654 T7655 nsubjpass These,probed
R4920 T7656 T7655 auxpass were,probed
R4921 T7657 T7655 prep with,probed
R4922 T7658 T7659 amod anti-MCAD,antibody
R4923 T7659 T7657 pobj antibody,with
R4924 T7660 T7659 cc or,antibody
R4925 T7661 T7662 amod anti-SCAD,antibody
R4926 T7662 T7659 conj antibody,antibody
R4927 T7663 T7655 punct .,probed
R4928 T7665 T7666 amod Homozygous,mice
R4929 T7666 T7671 nsubj mice,had
R4930 T7667 T7666 nmod MCAD,mice
R4931 T7668 T7667 punct −,MCAD
R4932 T7669 T7667 punct /,MCAD
R4933 T7670 T7667 punct −,MCAD
R4934 T7672 T7673 det no,protein
R4935 T7673 T7671 dobj protein,had
R4936 T7674 T7673 amod detectable,protein
R4937 T7675 T7673 compound MCAD,protein
R4938 T7676 T7671 punct .,had
R4939 T7678 T7679 compound MCAD,protein
R4940 T7679 T7680 nsubj protein,is
R4941 T7681 T7682 advmod only,detectable
R4942 T7682 T7680 acomp detectable,is
R4943 T7683 T7680 prep under,is
R4944 T7684 T7685 det the,lane
R4945 T7685 T7683 pobj lane,under
R4946 T7686 T7685 nmod MCAD,lane
R4947 T7687 T7688 npadvmod protein,spiked
R4948 T7688 T7685 amod spiked,lane
R4949 T7689 T7688 punct –,spiked
R4950 T7690 T7691 punct (,control
R4951 T7691 T7685 parataxis control,lane
R4952 T7692 T7691 amod positive,control
R4953 T7693 T7691 punct ),control
R4954 T7694 T7680 punct .,is
R4955 T7696 T7697 prep As,revealed
R4956 T7698 T7699 det a,analysis
R4957 T7699 T7696 pobj analysis,As
R4958 T7700 T7699 compound control,analysis
R4959 T7701 T7697 punct ", ",revealed
R4960 T7702 T7703 compound liver,homogenates
R4961 T7703 T7697 nsubj homogenates,revealed
R4962 T7704 T7703 acl probed,homogenates
R4963 T7705 T7704 prep with,probed
R4964 T7706 T7707 amod anti-SCAD,antibody
R4965 T7707 T7705 pobj antibody,with
R4966 T7708 T7709 mark that,was
R4967 T7709 T7697 ccomp was,revealed
R4968 T7710 T7711 compound SCAD,protein
R4969 T7711 T7709 nsubj protein,was
R4970 T7712 T7709 acomp present,was
R4971 T7713 T7709 prep in,was
R4972 T7714 T7715 preconj both,MCAD
R4973 T7715 T7716 nmod MCAD,mice
R4974 T7716 T7713 pobj mice,in
R4975 T7717 T7715 punct +,MCAD
R4976 T7718 T7715 punct /,MCAD
R4977 T7719 T7715 punct +,MCAD
R4978 T7720 T7715 cc and,MCAD
R4979 T7721 T7715 conj MCAD,MCAD
R4980 T7722 T7721 punct −,MCAD
R4981 T7723 T7721 punct /,MCAD
R4982 T7724 T7721 punct −,MCAD
R4983 T7725 T7697 punct .,revealed
R4984 T7727 T7728 det No,protein
R4985 T7728 T7733 nsubjpass protein,detected
R4986 T7729 T7728 nmod MCAD,protein
R4987 T7730 T7731 amod positive,control
R4988 T7731 T7728 compound control,protein
R4989 T7732 T7731 punct -,control
R4990 T7734 T7733 auxpass is,detected
R4991 T7735 T7733 prep by,detected
R4992 T7736 T7737 amod anti-SCAD,antibodies
R4993 T7737 T7735 pobj antibodies,by
R4994 T7738 T7739 punct (,lane
R4995 T7739 T7733 parataxis lane,detected
R4996 T7740 T7739 compound MCAD,lane
R4997 T7741 T7739 punct ),lane
R4998 T7742 T7733 punct .,detected
R4999 T7745 T7744 punct ", ",mw
R5000 T7746 T7747 amod molecular,standards
R5001 T7747 T7744 appos standards,mw
R5002 T7748 T7747 compound weight,standards
R5003 T7749 T7744 punct .,mw
R5004 T7814 T7815 compound Acylcarnitine,Analyses
R5005 T7817 T7818 punct (,A
R5006 T7818 T7819 meta A,analysis
R5007 T7820 T7818 punct ),A
R5008 T7821 T7822 compound Serum,acylcarnitine
R5009 T7822 T7819 compound acylcarnitine,analysis
R5010 T7823 T7819 prep of,analysis
R5011 T7824 T7825 nmod MCAD,mice
R5012 T7825 T7823 pobj mice,of
R5013 T7826 T7824 punct +,MCAD
R5014 T7827 T7824 punct /,MCAD
R5015 T7828 T7824 punct +,MCAD
R5016 T7829 T7830 punct (,4
R5017 T7830 T7824 parataxis 4,MCAD
R5018 T7831 T7830 nsubj n,4
R5019 T7832 T7830 punct =,4
R5020 T7833 T7830 punct ),4
R5021 T7834 T7824 cc and,MCAD
R5022 T7835 T7824 conj MCAD,MCAD
R5023 T7836 T7835 punct −,MCAD
R5024 T7837 T7835 punct /,MCAD
R5025 T7838 T7835 punct −,MCAD
R5026 T7839 T7840 punct (,4
R5027 T7840 T7825 parataxis 4,mice
R5028 T7841 T7840 nsubj n,4
R5029 T7842 T7840 punct =,4
R5030 T7843 T7840 punct ),4
R5031 T7845 T7846 expl There,are
R5032 T7847 T7848 amod significant,elevations
R5033 T7848 T7846 attr elevations,are
R5034 T7849 T7846 prep in,are
R5035 T7850 T7851 compound acylcarnitine,species
R5036 T7851 T7849 pobj species,in
R5037 T7852 T7853 mark as,indicated
R5038 T7853 T7846 advcl indicated,are
R5039 T7854 T7853 prep in,indicated
R5040 T7855 T7856 nmod MCAD,mice
R5041 T7856 T7854 pobj mice,in
R5042 T7857 T7855 punct −,MCAD
R5043 T7858 T7855 punct /,MCAD
R5044 T7859 T7855 punct −,MCAD
R5045 T7860 T7846 punct .,are
R5046 T7862 T7863 nsubj Values,are
R5047 T7864 T7862 acl shown,Values
R5048 T7865 T7866 compound mean,values
R5049 T7866 T7863 attr values,are
R5050 T7867 T7866 punct ±,values
R5051 T7868 T7869 amod standard,deviation
R5052 T7869 T7866 appos deviation,values
R5053 T7870 T7869 punct (,deviation
R5054 T7871 T7869 appos SD,deviation
R5055 T7872 T7863 punct ),are
R5056 T7873 T7863 punct .,are
R5057 T7875 T7876 nsubj Asterisk,indicates
R5058 T7877 T7876 dobj p,indicates
R5059 T7878 T7879 punct <,0.002
R5060 T7879 T7877 amod 0.002,p
R5061 T7880 T7876 cc and,indicates
R5062 T7881 T7882 nsubj ‡,indicates
R5063 T7882 T7876 conj indicates,indicates
R5064 T7883 T7882 dobj p,indicates
R5065 T7884 T7885 punct <,0.01
R5066 T7885 T7883 amod 0.01,p
R5067 T7886 T7882 punct .,indicates
R5068 T7888 T7889 punct (,B
R5069 T7889 T7890 meta B,are
R5070 T7891 T7889 punct ),B
R5071 T7892 T7890 expl There,are
R5072 T7893 T7894 amod significant,elevations
R5073 T7894 T7890 attr elevations,are
R5074 T7895 T7894 prep in,elevations
R5075 T7896 T7897 compound bile,acylcarnitines
R5076 T7897 T7895 pobj acylcarnitines,in
R5077 T7898 T7897 prep of,acylcarnitines
R5078 T7899 T7900 det the,mice
R5079 T7900 T7898 pobj mice,of
R5080 T7901 T7900 amod same,mice
R5081 T7902 T7900 acl shown,mice
R5082 T7903 T7902 prep in,shown
R5083 T7904 T7903 punct (,in
R5084 T7905 T7903 pobj A,in
R5085 T7906 T7902 punct ),shown
R5086 T7907 T7908 mark as,indicated
R5087 T7908 T7902 advcl indicated,shown
R5088 T7909 T7890 punct .,are
R5089 T7911 T7912 nsubj Values,are
R5090 T7913 T7911 acl shown,Values
R5091 T7914 T7915 compound mean,values
R5092 T7915 T7912 attr values,are
R5093 T7916 T7915 punct ±,values
R5094 T7917 T7915 appos SD,values
R5095 T7918 T7912 punct .,are
R5096 T7920 T7921 nsubj Asterisk,indicates
R5097 T7922 T7921 dobj p,indicates
R5098 T7923 T7924 punct <,0.001
R5099 T7924 T7922 amod 0.001,p
R5100 T7925 T7921 punct .,indicates
R5101 T7927 T7928 punct (,C
R5102 T7928 T7929 meta C,profile
R5103 T7930 T7928 punct ),C
R5104 T7931 T7932 compound Bile,acylcarnitine
R5105 T7932 T7929 compound acylcarnitine,profile
R5106 T7933 T7929 prep of,profile
R5107 T7934 T7935 det an,mouse
R5108 T7935 T7933 pobj mouse,of
R5109 T7936 T7935 nmod MCAD,mouse
R5110 T7937 T7936 punct −,MCAD
R5111 T7938 T7936 punct /,MCAD
R5112 T7939 T7936 punct −,MCAD
R5113 T7940 T7929 prep compared,profile
R5114 T7941 T7940 prep to,compared
R5115 T7942 T7943 det a,patient
R5116 T7943 T7941 pobj patient,to
R5117 T7944 T7943 amod human,patient
R5118 T7945 T7943 prep with,patient
R5119 T7946 T7947 compound MCAD,deficiency
R5120 T7947 T7945 pobj deficiency,with
R5121 T7948 T7929 punct .,profile
R5122 T7950 T7951 amod Internal,standards
R5123 T7951 T7952 nsubjpass standards,indicated
R5124 T7953 T7952 auxpass are,indicated
R5125 T7954 T7952 prep by,indicated
R5126 T7955 T7956 det an,asterisk
R5127 T7956 T7954 pobj asterisk,by
R5128 T7957 T7952 punct .,indicated
R5129 T8010 T8009 prep of,Histopathology
R5130 T8011 T8012 nmod MCAD,Mice
R5131 T8012 T8010 pobj Mice,of
R5132 T8013 T8011 punct +,MCAD
R5133 T8014 T8011 punct /,MCAD
R5134 T8015 T8011 punct +,MCAD
R5135 T8016 T8011 cc and,MCAD
R5136 T8017 T8011 conj MCAD,MCAD
R5137 T8018 T8017 punct −,MCAD
R5138 T8019 T8017 punct /,MCAD
R5139 T8020 T8017 punct −,MCAD
R5140 T8022 T8023 punct (,A
R5141 T8023 T8024 meta A,had
R5142 T8025 T8023 punct ),A
R5143 T8026 T8027 nmod MCAD,mice
R5144 T8027 T8024 nsubj mice,had
R5145 T8028 T8026 punct +,MCAD
R5146 T8029 T8026 punct /,MCAD
R5147 T8030 T8026 punct +,MCAD
R5148 T8031 T8032 det no,evidence
R5149 T8032 T8024 dobj evidence,had
R5150 T8033 T8032 prep of,evidence
R5151 T8034 T8035 amod hepatic,steatosis
R5152 T8035 T8033 pobj steatosis,of
R5153 T8036 T8024 prep following,had
R5154 T8037 T8038 det a,fast
R5155 T8038 T8036 pobj fast,following
R5156 T8039 T8040 nummod 24,h
R5157 T8040 T8038 compound h,fast
R5158 T8041 T8040 punct -,h
R5159 T8042 T8024 punct .,had
R5160 T8044 T8045 compound Liver,section
R5161 T8046 T8045 prep with,section
R5162 T8047 T8048 nmod Oil,O
R5163 T8048 T8051 compound O,stain
R5164 T8049 T8048 punct -,O
R5165 T8050 T8048 amod Red,O
R5166 T8051 T8046 pobj stain,with
R5167 T8052 T8045 punct .,section
R5168 T8054 T8055 punct (,B
R5169 T8055 T8056 meta B,Hepatosteatosis
R5170 T8057 T8055 punct ),B
R5171 T8058 T8056 prep in,Hepatosteatosis
R5172 T8059 T8060 compound MCAD,−
R5173 T8060 T8063 compound −,mouse
R5174 T8061 T8060 compound −,−
R5175 T8062 T8060 punct /,−
R5176 T8063 T8058 pobj mouse,in
R5177 T8064 T8056 prep following,Hepatosteatosis
R5178 T8065 T8066 det a,fast
R5179 T8066 T8064 pobj fast,following
R5180 T8067 T8068 nummod 24,h
R5181 T8068 T8066 compound h,fast
R5182 T8069 T8068 punct -,h
R5183 T8070 T8056 punct .,Hepatosteatosis
R5184 T8072 T8073 nmod Oil,O
R5185 T8073 T8076 npadvmod O,stained
R5186 T8074 T8073 punct -,O
R5187 T8075 T8073 amod Red,O
R5188 T8076 T8077 amod stained,sections
R5189 T8077 T8079 nsubj sections,revealed
R5190 T8078 T8077 compound liver,sections
R5191 T8080 T8081 amod severe,steatosis
R5192 T8081 T8079 dobj steatosis,revealed
R5193 T8082 T8080 cc and,severe
R5194 T8083 T8080 conj diffuse,severe
R5195 T8084 T8081 amod microvesicular,steatosis
R5196 T8085 T8084 cc and,microvesicular
R5197 T8086 T8084 conj macrovesicular,microvesicular
R5198 T8087 T8081 amod hepatic,steatosis
R5199 T8088 T8079 prep in,revealed
R5200 T8089 T8090 nmod MCAD,mice
R5201 T8090 T8088 pobj mice,in
R5202 T8091 T8089 punct −,MCAD
R5203 T8092 T8089 punct /,MCAD
R5204 T8093 T8089 punct −,MCAD
R5205 T8094 T8079 punct .,revealed
R5206 T8096 T8097 punct (,C
R5207 T8097 T8098 meta C,cardiomyopathy
R5208 T8099 T8097 cc and,C
R5209 T8100 T8097 conj D,C
R5210 T8101 T8100 punct ),D
R5211 T8102 T8098 amod Diffuse,cardiomyopathy
R5212 T8103 T8098 prep with,cardiomyopathy
R5213 T8104 T8105 amod chronic,degeneration
R5214 T8105 T8103 pobj degeneration,with
R5215 T8106 T8105 amod active,degeneration
R5216 T8107 T8105 amod multifocal,degeneration
R5217 T8108 T8105 compound myocyte,degeneration
R5218 T8109 T8105 cc and,degeneration
R5219 T8110 T8105 conj necrosis,degeneration
R5220 T8111 T8098 prep in,cardiomyopathy
R5221 T8112 T8113 nmod MCAD,mice
R5222 T8113 T8111 pobj mice,in
R5223 T8114 T8112 punct −,MCAD
R5224 T8115 T8112 punct /,MCAD
R5225 T8116 T8112 punct −,MCAD
R5226 T8117 T8098 punct .,cardiomyopathy
R5227 T8162 T8161 prep of,Characteristics
R5228 T8163 T8164 npadvmod MCAD,Deficient
R5229 T8164 T8166 amod Deficient,Mice
R5230 T8165 T8164 punct -,Deficient
R5231 T8166 T8162 pobj Mice,of
R1300 T2049 T2047 pobj mice,between
R1308 T2057 T2054 punct −,MCAD
R1309 T2058 T2059 punct (,Figure
R1310 T2059 T2022 parataxis Figure,revealed
R1311 T2060 T2059 nummod 3,Figure
R1312 T2061 T2059 punct ),Figure
R1313 T2062 T2022 punct .,revealed
R1314 T2064 T2065 compound Enzyme,activity
R1315 T2065 T2066 nsubjpass activity,analyzed
R1316 T2067 T2066 auxpass was,analyzed
R1317 T2068 T2066 prep in,analyzed
R1318 T2069 T2070 compound mouse,homogenates
R1319 T2070 T2068 pobj homogenates,in
R1320 T2071 T2070 compound liver,homogenates
R1321 T2072 T2066 advcl using,analyzed
R1322 T2073 T2074 det the,assay
R1323 T2074 T2072 dobj assay,using
R1324 T2075 T2076 nmod electron,flavoprotein
R1325 T2076 T2074 nmod flavoprotein,assay
R1326 T2077 T2076 nmod transport,flavoprotein
R1327 T2078 T2076 punct (,flavoprotein
R1328 T2079 T2076 appos ETF,flavoprotein
R1329 T2080 T2074 punct ),assay
R1330 T2081 T2074 compound reduction,assay
R1331 T2082 T2072 prep with,using
R1332 T2083 T2084 compound octanoyl,CoA
R1333 T2084 T2082 pobj CoA,with
R1334 T2085 T2084 punct -,CoA
R1335 T2086 T2087 punct (,C8
R1336 T2087 T2084 parataxis C8,CoA
R1337 T2088 T2087 punct :,C8
R1338 T2089 T2087 nummod 0,C8
R1339 T2090 T2087 punct ),C8
R2149 T3401 T3399 pobj h,for
R2150 T3402 T3401 punct -,h
R2151 T3403 T3379 punct ", ",were
R2152 T3404 T3379 expl there,were
R2153 T3405 T3379 attr minimal,were
R2154 T3406 T3405 prep to,minimal
R2155 T3407 T3406 pobj no,to
R2156 T3408 T3405 appos abnormalities,minimal
R2157 T3409 T3379 prep in,were
R2158 T3410 T3411 det all,organs
R2159 T3411 T3409 pobj organs,in
R2160 T3412 T3411 acl evaluated,organs
R2161 T3413 T3379 punct .,were
R2162 T3415 T3416 advmod Only,mice
R2163 T3416 T3423 nsubj mice,demonstrated
R2164 T3417 T3416 det the,mice
R2165 T3418 T3416 amod older,mice
R2166 T3419 T3416 nmod MCAD,mice
R2167 T3420 T3419 punct −,MCAD
R2168 T3421 T3419 punct /,MCAD
R2169 T3422 T3419 punct −,MCAD
R2170 T3424 T3423 punct ", ",demonstrated
R2171 T3425 T3423 advmod therefore,demonstrated
R2172 T3426 T3423 punct ", ",demonstrated
R2173 T3427 T3428 amod hepatic,steatosis
R2174 T3428 T3423 dobj steatosis,demonstrated
R2175 T3429 T3423 punct .,demonstrated
R2176 T3431 T3432 prep In,found
R2177 T3433 T3431 pobj addition,In
R2178 T3434 T3432 punct ", ",found
R2179 T3435 T3432 nsubj we,found
R2180 T3436 T3437 amod sporadic,lesions
R2181 T3437 T3432 dobj lesions,found
R2182 T3438 T3437 amod cardiac,lesions
R2183 T3439 T3432 prep in,found
R2184 T3440 T3441 amod multiple,mice
R2185 T3441 T3439 pobj mice,in
R2186 T3442 T3441 nmod MCAD,mice
R2191 T3448 T3449 prep In,had
R2192 T3450 T3451 nummod one,example
R2193 T3451 T3448 pobj example,In
R2194 T3452 T3449 punct ", ",had
R2195 T3453 T3454 det an,mouse
R2196 T3454 T3449 nsubj mouse,had
R2197 T3455 T3454 nmod MCAD,mouse
R2198 T3456 T3455 punct −,MCAD
R2199 T3457 T3455 punct /,MCAD
R2200 T3458 T3455 punct −,MCAD
R2201 T3459 T3460 amod diffuse,cardiomyopathy
R2202 T3460 T3449 dobj cardiomyopathy,had
R2203 T3461 T3460 prep with,cardiomyopathy
R2204 T3462 T3463 amod chronic,degeneration
R2205 T3463 T3461 pobj degeneration,with
R2206 T3464 T3463 amod active,degeneration
R2207 T3465 T3463 amod multifocal,degeneration
R2208 T3466 T3463 compound myocyte,degeneration
R2209 T3467 T3463 cc and,degeneration
R2210 T3468 T3463 conj necrosis,degeneration
R673 T1067 T1065 pobj region,of
R675 T1069 T1067 nmod exon,region
R676 T1070 T1069 nummod 8,exon
R677 T1071 T1064 prep in,results
R678 T1072 T1073 det the,formation
R679 T1073 T1071 pobj formation,in
R680 T1074 T1073 prep of,formation
R681 T1075 T1076 amod premature,codons
R682 T1076 T1074 pobj codons,of
R683 T1077 T1076 compound stop,codons
R684 T1078 T1073 acl resulting,formation
R685 T1079 T1078 prep in,resulting
R686 T1080 T1079 pobj truncation,in
R687 T1081 T1080 prep of,truncation
R688 T1082 T1083 det the,monomer
R689 T1083 T1081 pobj monomer,of
R739 T1138 T1139 compound ES,cell
R740 T1139 T1140 compound cell,clones
R741 T1140 T1141 nsubjpass clones,screened
R742 T1142 T1141 auxpass were,screened
R743 T1143 T1141 prep by,screened
R744 T1144 T1143 pobj PCR,by
R745 T1145 T1146 punct (,shown
R746 T1146 T1141 parataxis shown,screened
R747 T1147 T1146 nsubj data,shown
R748 T1148 T1146 neg not,shown
R749 T1149 T1146 punct ),shown
R750 T1150 T1141 cc and,screened
R751 T1151 T1141 conj confirmed,screened
R752 T1152 T1151 prep by,confirmed
R753 T1153 T1154 compound Southern,blot
R754 T1154 T1155 compound blot,analysis
R755 T1155 T1152 pobj analysis,by
R756 T1156 T1141 punct .,screened
R757 T1158 T1159 compound Southern,blot
R758 T1159 T1160 compound blot,analysis
R759 T1160 T1161 nsubj analysis,used
R760 T1162 T1163 det an,probe
R761 T1163 T1161 dobj probe,used
R762 T1164 T1163 nmod exon,probe
R763 T1165 T1164 nummod 10,exon
R764 T1166 T1167 punct (,A
R765 T1167 T1163 parataxis A,probe
R766 T1168 T1167 compound probe,A
R767 T1169 T1167 punct ),A
R768 T1170 T1163 punct ", ",probe
R769 T1171 T1172 neg not,present
R770 T1172 T1163 relcl present,probe
R771 T1173 T1172 prep in,present
R772 T1174 T1175 det the,vector
R773 T1175 T1173 pobj vector,in
R774 T1176 T1175 compound targeting,vector
R3035 T4573 T4560 advcl suggesting,is
R3036 T4574 T4575 det a,degree
R3037 T4575 T4573 dobj degree,suggesting
R3038 T4576 T4575 amod greater,degree
R3039 T4577 T4575 prep of,degree

craft-ca-core-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T242 326-331 NCBITaxon:10088 denotes mouse
T243 371-375 SO:0000704 denotes gene
T244 389-398 UBERON:0000922 denotes embryonic
T245 389-403 _FRAGMENT denotes embryonic stem
T246 409-414 CL:0002322 denotes cells
T247 405-407 _FRAGMENT denotes ES
T248 428-432 NCBITaxon:10088 denotes mice
T249 473-478 UBERON:0002107 denotes liver
T250 558-565 UBERON:0000948 denotes cardiac
T251 590-594 NCBITaxon:10088 denotes mice
T252 621-626 NCBITaxon:9606 denotes human
T253 820-825 NCBITaxon:10088 denotes mouse
T254 858-863 NCBITaxon:9606 denotes human
T255 941-951 CHEBI:35366 denotes fatty acid
T256 941-961 GO:0019395 denotes fatty acid oxidation
T257 952-961 MOP:0000568 denotes oxidation
T258 982-987 NCBITaxon:9606 denotes human
T259 1007-1017 CHEBI:35366 denotes fatty acid
T260 1007-1027 GO:0019395 denotes fatty acid oxidation
T261 1018-1027 MOP:0000568 denotes oxidation
T808 2375-2383 CHEBI:17984 denotes acyl-CoA
T809 2429-2450 CHEBI:61907 denotes Medium-chain acyl-CoA
T810 2477-2482 NCBITaxon:10088 denotes mouse
T811 2483-2487 SO:0000704 denotes gene
T812 2576-2591 MOP:0000590 denotes dehydrogenation
T813 2595-2614 _FRAGMENT denotes medium-chain length
T814 2624-2634 CHEBI:59554 denotes fatty acid
T815 2635-2645 CHEBI:51277 denotes thioesters
T816 2679-2686 GO:0005634 denotes nucleus
T817 2688-2698 GO:0006412 denotes translated
T818 2706-2713 GO:0005829 denotes cytosol
T819 2741-2761 GO:0005759 denotes mitochondrial matrix
T820 2785-2805 GO:0005759 denotes mitochondrial matrix
T821 2930-2940 CHEBI:35366 denotes fatty acid
T822 2930-2950 GO:0019395 denotes fatty acid oxidation
T823 2941-2950 MOP:0000568 denotes oxidation
T824 2988-2994 NCBITaxon:9606 denotes humans
T825 3001-3010 GO:0030849 denotes autosomal
T826 3461-3465 UBERON:0000104 denotes life
T827 3637-3640 GO:0016265 denotes die
T828 3720-3729 GO:0008152 denotes metabolic
T829 3847-3853 NCBITaxon:33208 denotes animal
T830 3996-4001 NCBITaxon:9606 denotes human
T831 4088-4093 NCBITaxon:10088 denotes mouse
T832 4122-4126 SO:0000704 denotes gene
T1450 4734-4740 SO:0000440 denotes vector
T1451 4767-4771 SO:0000147 denotes exon
T1452 4804-4815 GO:0006412 denotes Translation
T1453 4834-4838 SO:0000147 denotes exon
T1454 4876-4897 SO:0001587 denotes premature stop codons
T1455 4967-4987 SO:0001587 denotes premature stop codon
T1456 5001-5012 GO:0006412 denotes translation
T1457 5059-5063 SO:0000147 denotes exon
T1458 5120-5126 SO:0000417 denotes domain
T1459 5127-5136 SO:0001117 denotes α-helixes
T1460 5967-5974 CL:0002322 denotes ES cell
T1461 6092-6096 SO:0000147 denotes exon
T1462 6136-6152 SO:0001644 denotes targeting vector
T1463 6154-6164 GO:0097617 denotes hybridized
T1464 6262-6268 SO:0000440 denotes vector
T1465 6320-6327 CL:0002322 denotes ES cell
T1466 6409-6413 NCBITaxon:10088 denotes mice
T1467 6424-6428 NCBITaxon:10088 denotes mice
T1468 6474-6478 NCBITaxon:10088 denotes mice
T1469 6521-6525 NCBITaxon:10088 denotes mice
T1470 6855-6859 NCBITaxon:10088 denotes mice
T1471 7006-7011 GO:0016265 denotes death
T1472 7064-7070 SO:0001023 denotes allele
T1473 7213-7219 SO:0001023 denotes allele
T1474 7251-7256 NCBITaxon:10088 denotes mouse
T1475 7331-7338 SO:0001026 denotes genomic
T1476 7363-7367 NCBITaxon:10088 denotes mice
T1477 7473-7477 NCBITaxon:10088 denotes mice
T1827 7538-7542 SO:0000147 denotes exon
T1828 7562-7567 UBERON:0000948 denotes heart
T1829 7599-7608 SO:0000028 denotes base pair
T1830 7610-7612 SO:0000028 denotes bp
T1831 7646-7650 NCBITaxon:10088 denotes mice
T1832 7685-7689 NCBITaxon:10088 denotes mice
T1833 7799-7803 SO:0000147 denotes exon
T1834 7809-7813 SO:0000147 denotes exon
T1835 7826-7828 SO:0000028 denotes bp
T1836 7837-7844 SO:0000155 denotes plasmid
T1837 7866-7871 SO:0000147 denotes exons
T1838 7890-7897 SO:0000155 denotes plasmid
T1839 7967-7974 GO:0008380 denotes spliced
T1840 8032-8041 GO:0010467 denotes expressed
T1841 8049-8056 UBERON:0000479 denotes tissues
T1842 8079-8083 NCBITaxon:10088 denotes mice
T1843 8105-8115 GO:0010467 denotes expression
T1844 8129-8138 UBERON:0001348 denotes brown fat
T1845 8140-8146 UBERON:0002113 denotes kidney
T1846 8148-8153 UBERON:0000948 denotes heart
T1847 8155-8163 UBERON:0004288 denotes skeletal
T1848 8176-8181 UBERON:0002107 denotes liver
T1849 8195-8205 GO:0010467 denotes expression
T1850 8213-8218 UBERON:0000955 denotes brain
T1851 8220-8229 UBERON:0001347 denotes white fat
T1852 8235-8241 UBERON:0000473 denotes testes
T1853 8310-8317 GO:0008380 denotes spliced
T1854 8310-8317 _FRAGMENT denotes spliced
T1855 8324-8334 SO:1001187 denotes transcript
T1856 8345-8356 SO:0000673 denotes transcripts
T1857 8410-8414 NCBITaxon:10088 denotes mice
T1858 8529-8556 GO:0000184 denotes nonsense mediated RNA decay
T2183 8936-8941 UBERON:0002107 denotes Liver
T2184 8981-8986 UBERON:0002107 denotes liver
T2185 9018-9022 UBERON:0001977 denotes sera
T2186 9088-9092 NCBITaxon:10088 denotes mice
T2187 9113-9117 NCBITaxon:10088 denotes mice
T2188 9158-9178 CHEBI:61905 denotes short-chain acyl-CoA
T2189 9246-9256 GO:0010467 denotes expression
T2190 9301-9305 NCBITaxon:10088 denotes mice
T2191 9890-9895 NCBITaxon:10088 denotes mouse
T2192 9896-9901 UBERON:0002107 denotes liver
T2193 9924-9932 CHEBI:10545 denotes electron
T2194 9924-9942 MOP:0000615 denotes electron transport
T2195 9924-9955 GO:0045251 denotes electron transport flavoprotein
T2196 9943-9955 CHEBI:5086 denotes flavoprotein
T2197 9957-9960 GO:0045251 denotes ETF
T2198 9962-9971 MOP:0000569 denotes reduction
T2199 9983-9995 CHEBI:15533 denotes octanoyl-CoA
T2200 10007-10020 CHEBI:15525 denotes palmitoyl-CoA
T2201 10052-10056 NCBITaxon:10088 denotes mice
T2202 10099-10112 MOP:0000590 denotes dehydrogenate
T2203 10113-10125 CHEBI:15533 denotes octanoyl-CoA
T2204 10168-10181 CHEBI:15525 denotes palmitoyl-CoA
T2205 10202-10206 NCBITaxon:10088 denotes mice
T2206 10236-10251 MOP:0000590 denotes dehydrogenation
T2207 10255-10267 CHEBI:15533 denotes octanoyl-CoA
T2208 10272-10285 CHEBI:15525 denotes palmitoyl-CoA
T2209 10354-10358 NCBITaxon:10088 denotes mice
T7153 28088-28099 CHEBI:51686 denotes hematoxylin
T7397 5278-5283 NCBITaxon:10088 denotes Mouse
T7398 5290-5294 SO:0000704 denotes Gene
T7399 5322-5338 SO:0001644 denotes targeting vector
T7400 5381-5385 SO:0000147 denotes exon
T7401 5393-5401 SO:0000357 denotes flanking
T7402 5533-5538 SO:0000147 denotes exons
T7403 5569-5575 SO:0001023 denotes allele
T7404 5622-5629 SO:0001026 denotes genomic
T7405 5639-5647 CL:0002322 denotes ES cells
T7406 5705-5720 SO:0000852 denotes portion of exon
T7407 5751-5767 SO:0001644 denotes targeting vector
T7408 5769-5779 GO:0097617 denotes hybridizes
T7409 5902-5909 CL:0002322 denotes ES cell
T7410 5952-5959 CL:0002322 denotes ES cell
T7550 8632-8636 NCBITaxon:10088 denotes Mice
T7551 8643-8650 SO:0000673 denotes message
T7552 8643-8650 CHEBI:33695 denotes message
T7553 8673-8678 UBERON:0000948 denotes heart
T7554 8680-8685 UBERON:0002107 denotes liver
T7555 8687-8696 UBERON:0001348 denotes brown fat
T7556 8698-8703 UBERON:0000955 denotes brain
T7557 8705-8711 UBERON:0002113 denotes kidney
T7558 8729-8738 UBERON:0001347 denotes white fat
T7559 8743-8749 UBERON:0000473 denotes testes
T7560 8783-8787 NCBITaxon:10088 denotes mice
T7561 8801-8811 GO:0010467 denotes expression
T7562 8824-8833 UBERON:0001348 denotes brown fat
T7563 8835-8841 UBERON:0002113 denotes kidney
T7564 8843-8848 UBERON:0000948 denotes heart
T7565 8854-8862 UBERON:0004288 denotes skeletal
T7566 8879-8883 NCBITaxon:10088 denotes mice
T7567 8902-8909 SO:0000673 denotes message
T7568 8902-8909 CHEBI:33695 denotes message
T7569 8917-8924 UBERON:0000479 denotes tissues
T7750 9343-9348 UBERON:0002107 denotes Liver
T7751 9386-9390 NCBITaxon:10088 denotes Mice
T7752 9424-9432 GO:0042571 denotes antibody
T7753 9446-9454 GO:0042571 denotes antibody
T7754 9475-9479 NCBITaxon:10088 denotes mice
T7755 9622-9627 UBERON:0002107 denotes liver
T7756 9662-9670 GO:0042571 denotes antibody
T7757 9738-9742 NCBITaxon:10088 denotes mice
T7758 9802-9812 GO:0042571 denotes antibodies
T8129 15438-15442 NCBITaxon:10088 denotes mice
T8128 15393-15400 CL:0000187 denotes myocyte
T8127 15323-15327 NCBITaxon:10088 denotes mice
T8126 15294-15301 UBERON:0002107 denotes hepatic
T8125 15284-15293 GO:0031982 denotes vesicular
T8124 15265-15274 GO:0031982 denotes vesicular
T8123 15217-15222 UBERON:0002107 denotes liver
T8122 15170-15175 NCBITaxon:10088 denotes mouse
T8121 15103-15108 UBERON:0002107 denotes Liver
T8120 15062-15069 UBERON:0002107 denotes hepatic
T8119 15038-15042 NCBITaxon:10088 denotes mice
T8118 15021-15025 NCBITaxon:10088 denotes Mice
T7970 14322-14327 NCBITaxon:9606 denotes human
T7969 14302-14307 NCBITaxon:10088 denotes mouse
T7968 14266-14279 CHEBI:17387 denotes acylcarnitine
T7967 14261-14265 UBERON:0001970 denotes Bile
T7966 14160-14164 NCBITaxon:10088 denotes mice
T7965 14133-14147 CHEBI:17387 denotes acylcarnitines
T7964 14128-14132 UBERON:0001970 denotes bile
T7963 13971-13975 NCBITaxon:10088 denotes mice
T7962 13925-13938 CHEBI:17387 denotes acylcarnitine
T7961 13876-13880 NCBITaxon:10088 denotes mice
T7960 13822-13835 CHEBI:17387 denotes acylcarnitine
T7959 13816-13821 UBERON:0001977 denotes Serum
T7958 13789-13802 CHEBI:17387 denotes Acylcarnitine
T7157 28392-28399 UBERON:0000948 denotes cardiac
T7156 28353-28360 UBERON:0000948 denotes cardiac
T7155 28277-28281 NCBITaxon:10088 denotes mice
T7154 28118-28123 UBERON:0002107 denotes liver
T7152 27997-28005 CHEBI:16842 denotes formalin
T7151 27941-27949 UBERON:0004288 denotes skeletal
T7150 27928-27935 UBERON:0000992 denotes ovaries
T7149 27917-27926 UBERON:0000473 denotes testicles
T7148 27910-27915 UBERON:0000948 denotes heart
T7147 27903-27908 UBERON:0000955 denotes brain
T7146 27896-27901 UBERON:0002107 denotes liver
T7145 27886-27894 UBERON:0001264 denotes pancreas
T7144 27878-27884 UBERON:0002106 denotes spleen
T7143 27870-27876 UBERON:0002113 denotes Kidney
T7142 27855-27859 NCBITaxon:10088 denotes mice
T7141 27804-27808 NCBITaxon:10088 denotes mice
T7140 27756-27761 NCBITaxon:10088 denotes mouse
T7139 27707-27712 NCBITaxon:10088 denotes mouse
T7138 27606-27610 NCBITaxon:10088 denotes mice
T6923 27509-27513 UBERON:0001970 denotes bile
T6922 27499-27504 UBERON:0001977 denotes serum
T6921 27473-27486 CHEBI:17387 denotes Acylcarnitine
T6920 27277-27288 CHEBI:32936 denotes tetracosane
T6919 27157-27169 CHEBI:64709 denotes organic acid
T6918 27151-27156 UBERON:0001088 denotes Urine
T6917 26942-26950 CHEBI:33893 denotes reagents
T6916 26862-26873 CHEBI:35366 denotes fatty acids
T6915 26725-26729 UBERON:0001977 denotes sera
T6914 26681-26688 CHEBI:17234 denotes Glucose
T6913 26639-26648 CHEBI:17126 denotes carnitine
T6912 26621-26633 CHEBI:64709 denotes organic acid
T6911 26609-26619 CHEBI:35366 denotes fatty acid
T6910 26595-26602 CHEBI:17234 denotes glucose
T6909 26589-26594 UBERON:0001977 denotes serum
T6908 26525-26529 NCBITaxon:10088 denotes mice
T6509 25954-25962 GO:0042571 denotes antibody
T6508 25933-25943 MOP:0000779 denotes conjugated
T6507 25925-25928 GO:0071735 denotes IgG
T6506 25919-25924 NCBITaxon:10088 denotes mouse
T6505 25880-25888 GO:0042571 denotes antibody
T6504 25785-25793 CHEBI:53424 denotes Tween-20
T6503 25741-25745 UBERON:0001913 denotes milk
T6502 25624-25638 CHEBI:53325 denotes nitrocellulose
T6501 25573-25576 CHEBI:8984 denotes SDS
T6500 25401-25421 CHEBI:35607 denotes sodium orthovanadate
T6499 25359-25387 CHEBI:8102 denotes phenylmethylsulfonylfluoride
T6498 25238-25246 CHEBI:17754 denotes glycerol
T6497 25224-25227 CHEBI:8984 denotes SDS
T6496 25196-25215 CHEBI:9177 denotes sodium deoxycholate
T6495 25177-25189 CHEBI:78708 denotes Nonidet P-40
T6494 25144-25149 GO:0019835 denotes lysed
T6493 25117-25123 UBERON:0000479 denotes tissue
T6492 25033-25037 NCBITaxon:10088 denotes mice
T6491 24967-24972 UBERON:0002107 denotes liver
T6490 24959-24965 UBERON:0000479 denotes tissue
T6489 24953-24958 NCBITaxon:10088 denotes mouse
T6651 26400-26413 CHEBI:15525 denotes palmitoyl-CoA
T6650 26378-26390 CHEBI:15533 denotes octanoyl-CoA
T6649 26357-26363 UBERON:0000479 denotes tissue
T6648 26329-26338 MOP:0000569 denotes reduction
T6647 26325-26328 GO:0045251 denotes ETF
T6646 26267-26271 NCBITaxon:10088 denotes mice
T6645 26202-26207 UBERON:0002107 denotes liver
T6644 26196-26200 NCBITaxon:10088 denotes mice
T6108 24825-24841 CHEBI:4883 denotes ethidium bromide
T6107 24791-24803 CHEBI:16842 denotes formaldehyde
T6106 24783-24790 CHEBI:2511 denotes agarose
T6105 24612-24615 CHEBI:8984 denotes SDS
T6104 24578-24581 CHEBI:8984 denotes SDS
T6103 24523-24533 GO:0097617 denotes hybridized
T6102 24498-24503 CL:0000019 denotes sperm
T6101 24482-24485 CHEBI:8984 denotes SDS
T6100 24470-24477 CHEBI:33893 denotes reagent
T6099 24439-24454 CHEBI:34674 denotes dextran sulfate
T6098 24424-24433 CHEBI:16397 denotes formamide
T6097 24338-24352 GO:0097617 denotes Hybridizations
T6096 24283-24288 NCBITaxon:10088 denotes mouse
T6095 24254-24257 CHEBI:37972 denotes 32P
T6094 24238-24248 GO:0097617 denotes hybridized
T6093 24119-24133 CHEBI:53325 denotes nitrocellulose
T6092 24086-24098 CHEBI:16842 denotes formaldehyde
T6091 24078-24085 CHEBI:2511 denotes agarose
T6090 23884-23888 NCBITaxon:10088 denotes mice
T6089 23845-23851 UBERON:0000473 denotes testes
T6088 23830-23839 UBERON:0001347 denotes white fat
T6087 23813-23821 UBERON:0004288 denotes skeletal
T6086 23805-23811 UBERON:0002113 denotes kidney
T6085 23798-23803 UBERON:0000955 denotes brain
T6084 23787-23796 UBERON:0001348 denotes brown fat
T6083 23780-23785 UBERON:0002107 denotes liver
T6082 23773-23778 UBERON:0000948 denotes heart
T6081 23701-23705 NCBITaxon:10088 denotes mice
T6080 23674-23683 GO:0010467 denotes expressed
T6079 23574-23580 SO:0000440 denotes vector
T6078 23426-23430 SO:0000147 denotes exon
T6077 23415-23419 SO:0000147 denotes exon
T6076 23194-23215 GO:0001171 denotes Reverse transcription
T6075 23169-23180 CHEBI:18022 denotes thiocyanate
T6074 23157-23168 CHEBI:30087 denotes guanidinium
T6073 23123-23127 NCBITaxon:10088 denotes mice
T6072 23073-23078 UBERON:0000948 denotes heart
T5568 23018-23024 UBERON:0000062 denotes organs
T5567 22937-22942 UBERON:0001153 denotes cecum
T5566 22921-22932 UBERON:0001728 denotes nasopharynx
T5565 22899-22908 NCBITaxon:2 denotes bacterial
T5564 22882-22889 NCBITaxon:10239 denotes viruses
T5563 22820-22825 NCBITaxon:10088 denotes mouse
T5562 22797-22801 NCBITaxon:10088 denotes Mice
T5561 22791-22795 NCBITaxon:10088 denotes mice
T5560 22711-22715 NCBITaxon:10088 denotes mice
T5559 22697-22701 SO:0000704 denotes gene
T5558 22681-22685 NCBITaxon:10088 denotes mice
T5557 22630-22634 NCBITaxon:10088 denotes mice
T5556 22615-22619 NCBITaxon:10088 denotes mice
T5555 22582-22593 UBERON:0000358 denotes blastocysts
T5554 22540-22547 CL:0002322 denotes ES cell
T5553 22469-22476 SO:0001026 denotes genomic
T5552 22446-22455 GO:0097617 denotes hybridize
T5551 22419-22425 SO:0000440 denotes vector
T5550 22352-22358 SO:0000188 denotes intron
T5549 22341-22345 SO:0000147 denotes exon
T5548 22294-22296 SO:0000028 denotes bp
T5547 22208-22210 CL:0002322 denotes ES
T5546 22193-22197 CHEBI:42768 denotes G418
T5545 22176-22183 SO:0001026 denotes Genomic
T5544 22035-22063 GO:0006302 denotes double stranded–break repair
T5543 22035-22050 SO:0000985 denotes double stranded
T5542 21965-21971 SO:0000440 denotes vector
T5541 21924-21928 NCBITaxon:10088 denotes mice
T5540 21868-21876 CL:0002322 denotes ES cells
T5539 21811-21818 SO:0001026 denotes genomic
T5538 21747-21753 SO:0000440 denotes vector
T5537 21721-21725 NCBITaxon:10088 denotes mice
T5203 21663-21667 SO:0000147 denotes exon
T5202 21562-21570 GO:0006266 denotes ligation
T5201 21535-21541 SO:0000440 denotes vector
T5200 21417-21425 GO:0006266 denotes ligation
T5199 21344-21351 SO:0001026 denotes genomic
T5198 21332-21337 PR:P23940 denotes BamHI
T5197 21305-21311 SO:0000440 denotes vector
T5196 21274-21280 SO:0000188 denotes intron
T5195 21265-21273 SO:0000357 denotes flanking
T5194 21253-21257 SO:0000147 denotes exon
T5193 21225-21232 SO:0001026 denotes genomic
T5192 21213-21218 PR:P23940 denotes BamHI
T5191 21178-21183 PR:P23940 denotes BamHI
T5190 21153-21159 SO:0000440 denotes vector
T5189 21054-21061 SO:0001026 denotes genomic
T5188 21048-21053 NCBITaxon:10088 denotes mouse
T5187 20984-20990 SO:0000188 denotes intron
T5186 20975-20983 SO:0000357 denotes flanking
T5185 20952-20957 SO:0000147 denotes exons
T5184 20926-20933 SO:0001026 denotes genomic
T5183 20897-20904 SO:0000155 denotes plasmid
T5182 20820-20826 SO:0000440 denotes vector
T5181 20774-20782 GO:0006266 denotes ligation
T5180 20681-20688 SO:0000155 denotes plasmid
T5179 20609-20622 SO:0005853 denotes gene cassette
T5178 20589-20597 CHEBI:7507 denotes neomycin
T5177 20569-20585 SO:0001644 denotes targeting vector
T4690 18382-18386 NCBITaxon:10088 denotes mice
T4689 18279-18283 NCBITaxon:10088 denotes mice
T4688 18265-18270 UBERON:0007023 denotes adult
T4687 18216-18224 GO:0007631 denotes ingested
T4686 18204-18208 UBERON:0001913 denotes milk
T4685 18057-18061 UBERON:0000104 denotes life
T4684 17901-17906 NCBITaxon:9606 denotes Human
T4683 17862-17867 GO:0016265 denotes dying
T4682 17773-17777 NCBITaxon:10088 denotes mice
T4681 17626-17630 NCBITaxon:10088 denotes mice
T4680 17554-17562 CHEBI:17984 denotes acyl-CoA
T4679 17418-17422 NCBITaxon:10088 denotes mice
T4678 17390-17397 CHEBI:59132 denotes antigen
T4677 17333-17346 CHEBI:15525 denotes palmitoyl-CoA
T4676 17305-17308 CHEBI:15346 denotes CoA
T4675 17238-17243 NCBITaxon:9606 denotes human
T4674 17215-17218 CHEBI:15346 denotes CoA
T4673 17198-17211 MOP:0000590 denotes dehydrogenate
T4672 17154-17158 NCBITaxon:10088 denotes mice
T4671 17117-17121 NCBITaxon:10088 denotes mice
T4670 17056-17061 UBERON:0002107 denotes liver
T4669 17036-17045 MOP:0000569 denotes reduction
T4668 17032-17035 GO:0045251 denotes ETF
T4667 17010-17014 CHEBI:22221 denotes acyl
T4666 16954-16959 NCBITaxon:9606 denotes human
T4665 16923-16927 CHEBI:22221 denotes acyl
T4664 16885-16890 NCBITaxon:10088 denotes mouse
T4663 16832-16856 CHEBI:61910 denotes very long-chain acyl-CoA
T4662 16771-16776 NCBITaxon:9606 denotes human
T4661 16705-16710 NCBITaxon:10088 denotes mouse
T4660 16662-16675 CHEBI:17387 denotes Acylcarnitine
T4659 16656-16660 NCBITaxon:10088 denotes mice
T4658 16611-16626 CHEBI:64390 denotes hexanoylglycine
T4657 16603-16610 UBERON:0001088 denotes urinary
T4656 16471-16482 CHEBI:25212 denotes metabolites
T4655 16465-16470 UBERON:0001088 denotes urine
T4654 16454-16460 UBERON:0001969 denotes plasma
T4653 16400-16405 NCBITaxon:9606 denotes human
T4652 16282-16287 NCBITaxon:10088 denotes mouse
T3606 15762-15768 GO:0005634 denotes Nuclei
T3616 16214-16228 UBERON:0001013 denotes adipose tissue
T3615 16121-16128 CHEBI:26130 denotes pigment
T3614 16038-16044 UBERON:0000479 denotes tissue
T3613 15983-15988 UBERON:0000948 denotes heart
T3612 15958-15963 UBERON:0004663 denotes aorta
T3611 15946-15953 _FRAGMENT denotes wall of
T3610 15905-15913 CL:0000187 denotes myocytes
T3609 15840-15848 GO:0005730 denotes nucleoli
T3608 15812-15821 GO:0031982 denotes vesicular
T3607 15781-15789 CL:0000187 denotes myocytes
T3605 15739-15751 CL:0000771 denotes eosinophilic
T3604 15707-15717 GO:0030016 denotes myofibrils
T3603 15680-15690 GO:0016528 denotes sarcoplasm
T3602 15613-15621 CL:0000187 denotes myocytes
T3601 15535-15542 CL:0000187 denotes myocyte
T3600 15471-15476 NCBITaxon:10088 denotes mouse
T3599 14967-14971 NCBITaxon:10088 denotes mice
T3598 14931-14938 UBERON:0000948 denotes cardiac
T3597 14881-14888 UBERON:0002107 denotes hepatic
T3596 14851-14855 NCBITaxon:10088 denotes mice
T3595 14810-14816 UBERON:0000062 denotes organs
T3594 14742-14746 NCBITaxon:10088 denotes mice
T3593 14642-14646 NCBITaxon:10088 denotes mice
T3592 14621-14625 NCBITaxon:10088 denotes mice
T3591 14581-14588 UBERON:0002107 denotes hepatic
T3590 14571-14580 GO:0031982 denotes vesicular
T3589 14552-14561 GO:0031982 denotes vesicular
T3588 14503-14507 NCBITaxon:10088 denotes mice
T3218 13764-13777 CHEBI:17387 denotes acylcarnitine
T3217 13745-13750 NCBITaxon:10088 denotes mouse
T3216 13722-13728 NCBITaxon:9606 denotes humans
T3215 13705-13718 CHEBI:17387 denotes acylcarnitine
T3214 13662-13666 NCBITaxon:10088 denotes mice
T3213 13628-13642 CHEBI:17387 denotes acylcarnitines
T3212 13563-13568 NCBITaxon:9606 denotes Human
T3211 13520-13525 NCBITaxon:9606 denotes human
T3210 13487-13491 NCBITaxon:10088 denotes mice
T3209 13449-13462 CHEBI:17387 denotes acylcarnitine
T3208 13417-13422 UBERON:0001977 denotes serum
T3207 13389-13402 CHEBI:17387 denotes acylcarnitine
T3206 13347-13360 CHEBI:17387 denotes acylcarnitine
T3205 13342-13346 UBERON:0001970 denotes Bile
T3204 13307-13320 CHEBI:17387 denotes acylcarnitine
T3203 13274-13291 CHEBI:86063 denotes decenoylcarnitine
T3202 13268-13273 UBERON:0001977 denotes serum
T3201 13231-13235 NCBITaxon:10088 denotes mice
T3200 13213-13217 NCBITaxon:10088 denotes mice
T3199 13160-13169 CHEBI:17126 denotes carnitine
T3198 13154-13159 UBERON:0001977 denotes serum
T3197 13104-13108 NCBITaxon:10088 denotes mice
T3196 12965-12976 CHEBI:15344 denotes acetoacetic
T3195 12944-12960 CHEBI:20067 denotes β-hydroxybutyric
T3194 12878-12889 CHEBI:30832 denotes Adipic acid
T3193 12853-12866 CHEBI:64709 denotes organic acids
T3192 12748-12763 CHEBI:64390 denotes hexanoylglycine
T3191 12730-12743 CHEBI:41865 denotes sebacic acids
T3190 12738-12743 CHEBI:9300 denotes acids
T3189 12717-12724 _FRAGMENT denotes suberic
T3188 12738-12743 CHEBI:30832 denotes acids
T3187 12709-12715 _FRAGMENT denotes adipic
T3186 12653-12657 NCBITaxon:10088 denotes mice
T3185 12593-12598 NCBITaxon:9606 denotes human
T3184 12546-12558 CHEBI:64709 denotes organic acid
T2479 11408-11415 GO:0007618 denotes matings
T2478 11334-11341 GO:0007618 denotes matings
T2477 11303-11310 GO:0007618 denotes matings
T2476 10942-10946 GO:0016265 denotes loss
T2475 10900-10904 NCBITaxon:10088 denotes mice
T2474 10845-10849 NCBITaxon:10088 denotes mice
T2473 10819-10823 NCBITaxon:10088 denotes mice
T2472 10789-10793 GO:0007567 denotes born
T2471 10688-10694 GO:0016265 denotes Deaths
T2820 12406-12412 UBERON:0001052 denotes rectal
T2819 12348-12352 NCBITaxon:10088 denotes mice
T2818 12295-12299 NCBITaxon:10088 denotes mice
T2817 12233-12237 NCBITaxon:10088 denotes mice
T2816 12129-12135 UBERON:0001052 denotes Rectal
T2815 12115-12119 NCBITaxon:10088 denotes mice
T2814 12055-12059 NCBITaxon:10088 denotes mice
T2813 12025-12031 UBERON:0001052 denotes rectal
T2812 11859-11863 NCBITaxon:10088 denotes mice
T2811 11772-11776 NCBITaxon:10088 denotes mice
T2810 11715-11719 NCBITaxon:10088 denotes mice
T2809 11633-11643 CHEBI:35366 denotes fatty acid
T2808 11622-11627 UBERON:0001977 denotes serum
T2807 11601-11608 CHEBI:17234 denotes glucose
T2806 11595-11600 UBERON:0001977 denotes serum
T2805 11574-11578 NCBITaxon:10088 denotes mice
T2804 11515-11519 NCBITaxon:10088 denotes mice
T8169 10435-10439 NCBITaxon:10088 denotes Mice
T6643 26135-26140 UBERON:0002107 denotes Liver
T233 0-21 CHEBI:61907 denotes Medium-Chain Acyl-CoA
T234 50-54 SO:0000704 denotes Gene
T235 64-68 NCBITaxon:10088 denotes Mice
T236 69-134 CHEBI:61907 denotes MCAD Gene Disruption in the Mouse Abstract Medium-chain acyl-CoA
T237 208-221 GO:0005739 denotes mitochondrial
T238 222-232 CHEBI:35366 denotes fatty acid
T239 222-244 GO:0006635 denotes fatty acid β-oxidation
T240 235-244 MOP:0000568 denotes oxidation
T241 248-254 NCBITaxon:9606 denotes humans
T3183 12528-12532 NCBITaxon:10088 denotes mice
T3182 12484-12496 CHEBI:64709 denotes organic acid
T3181 12478-12483 UBERON:0001088 denotes Urine
T3180 12455-12468 CHEBI:17387 denotes Acylcarnitine
T3179 12438-12450 CHEBI:64709 denotes Organic Acid
T800 2143-2156 GO:0005739 denotes Mitochondrial
T801 2157-2183 GO:0006635 denotes β-oxidation of fatty acids
T802 2159-2168 MOP:0000568 denotes oxidation
T803 2172-2183 CHEBI:35366 denotes fatty acids
T804 2239-2249 CHEBI:35366 denotes Fatty acid
T805 2239-2259 GO:0019395 denotes Fatty acid oxidation
T806 2250-2259 MOP:0000568 denotes oxidation
T807 2270-2282 GO:0005739 denotes mitochondria
T833 4140-4149 UBERON:0000922 denotes embryonic
T834 4140-4154 _FRAGMENT denotes embryonic stem
T835 4160-4165 CL:0002322 denotes cells
T836 4156-4158 _FRAGMENT denotes ES
T837 4200-4204 NCBITaxon:10088 denotes mice
T838 4271-4276 NCBITaxon:9606 denotes human
T1443 4349-4353 SO:0000704 denotes Gene
T1444 4397-4401 NCBITaxon:10088 denotes Mice
T1445 4417-4423 SO:0000440 denotes vector
T1446 4553-4561 CL:0002322 denotes ES cells
T1447 4634-4639 SO:0000147 denotes exons
T1448 4672-4680 SO:0000357 denotes flanking
T1449 4681-4688 SO:0000155 denotes plasmid
T4735 20484-20495 CHEBI:35366 denotes fatty acids
T4734 20397-20406 MOP:0000568 denotes oxidation
T4733 20381-20394 GO:0005739 denotes mitochondrial
T4732 20330-20335 NCBITaxon:10088 denotes mouse
T4731 20272-20279 NCBITaxon:33208 denotes animals
T4730 20240-20244 NCBITaxon:10088 denotes mice
T4729 20112-20117 NCBITaxon:10088 denotes mouse
T4728 20075-20079 NCBITaxon:10088 denotes mice
T4727 19976-19987 GO:0007565 denotes gestational
T4726 19965-19971 GO:0016265 denotes deaths
T4725 19938-19942 NCBITaxon:10088 denotes mice
T4724 19922-19928 UBERON:0002113 denotes kidney
T4723 19911-19916 UBERON:0000948 denotes heart
T4722 19904-19909 UBERON:0002107 denotes liver
T4721 19790-19795 NCBITaxon:10088 denotes mouse
T4720 19735-19739 NCBITaxon:10088 denotes mice
T4719 19688-19692 NCBITaxon:10088 denotes mice
T4718 19618-19626 CHEBI:17984 denotes acyl-CoA
T4717 19601-19606 NCBITaxon:10088 denotes mouse
T4716 19535-19541 NCBITaxon:9606 denotes humans
T4715 19419-19423 NCBITaxon:10088 denotes mice
T4714 19394-19401 UBERON:0000948 denotes cardiac
T4713 19384-19389 UBERON:0002107 denotes liver
T4712 19355-19360 NCBITaxon:10088 denotes mouse
T4711 19291-19296 NCBITaxon:10088 denotes mouse
T4710 19267-19274 UBERON:0000948 denotes cardiac
T4709 19212-19214 PR:000005838 denotes -2
T4708 19202-19205 _FRAGMENT denotes CPT
T4707 19202-19207 PR:000005835 denotes CPT-1
T4706 19161-19186 GO:0001676 denotes long chain fat metabolism
T4705 19161-19175 CHEBI:15904 denotes long chain fat
T4704 18973-18980 UBERON:0000948 denotes cardiac
T4703 18943-18948 NCBITaxon:9606 denotes human
T4702 18913-18917 NCBITaxon:10088 denotes mice
T4701 18859-18866 CL:0000187 denotes myocyte
T4700 18757-18761 NCBITaxon:10088 denotes mice
T4699 18730-18737 UBERON:0000948 denotes cardiac
T4698 18680-18685 NCBITaxon:9606 denotes human
T4697 18615-18619 NCBITaxon:10088 denotes mice
T4696 18574-18581 UBERON:0002107 denotes hepatic
T4695 18564-18573 GO:0031982 denotes vesicular
T4694 18545-18554 GO:0031982 denotes vesicular
T4693 18499-18508 GO:0010467 denotes expresses
T4692 18472-18485 GO:0031649 denotes thermogenesis
T4691 18429-18438 UBERON:0001348 denotes Brown fat
R118 T246 T245 _lexicallyChainedTo cells,embryonic stem
R119 T246 T247 _lexicallyChainedTo cells,ES
R573 T814 T813 _lexicallyChainedTo fatty acid,medium-chain length
R574 T835 T834 _lexicallyChainedTo cells,embryonic stem
R575 T835 T836 _lexicallyChainedTo cells,ES
R1233 T1855 T1854 _lexicallyChainedTo transcript,spliced
R2066 T3188 T3187 _lexicallyChainedTo acids,adipic
R2067 T3190 T3189 _lexicallyChainedTo acids,suberic
R2320 T3612 T3611 _lexicallyChainedTo aorta,wall of
R3110 T4709 T4708 _lexicallyChainedTo -2,CPT

2_test

Id Subject Object Predicate Lexical cue
16121256-11826276-86078317 2647-2648 11826276 denotes 1
16121256-3611054-86078318 2763-2764 3611054 denotes 2
16121256-3035565-86078318 2763-2764 3035565 denotes 2
16121256-3813556-86078318 2763-2764 3813556 denotes 2
16121256-3813556-86078319 2886-2887 3813556 denotes 4
16121256-7172449-86078320 3081-3082 7172449 denotes 5
16121256-11826276-86078321 3131-3132 11826276 denotes 1
16121256-1438385-86078322 3133-3134 1438385 denotes 8
16121256-1601002-86078322 3133-3134 1601002 denotes 8
16121256-11826276-86078323 3307-3308 11826276 denotes 1
16121256-1601002-86078324 3309-3310 1601002 denotes 9
16121256-11826276-86078325 3533-3534 11826276 denotes 1
16121256-11826276-86078326 3678-3679 11826276 denotes 1
16121256-2660260-86078327 4180-4182 2660260 denotes 13
16121256-1591000-86078328 4183-4185 1591000 denotes 14
16121256-3054550-86078329 16568-16570 3054550 denotes 15
16121256-11590124-86078330 17027-17029 11590124 denotes 16
16121256-11826276-86078331 18063-18064 11826276 denotes 1
16121256-12897989-86078332 19056-19058 12897989 denotes 17
16121256-15858970-86078333 19060-19062 15858970 denotes 18
16121256-9498103-86078334 19134-19136 9498103 denotes 19
16121256-11826276-86078335 19229-19230 11826276 denotes 1
16121256-11826276-86078336 19543-19544 11826276 denotes 1
16121256-3786030-86078337 19545-19547 3786030 denotes 20
16121256-11590124-86078338 19804-19806 11590124 denotes 16
16121256-9861014-86078339 19807-19809 9861014 denotes 21
16121256-2919115-86078340 19810-19812 2919115 denotes 22
16121256-9861014-86078341 20024-20026 9861014 denotes 21
16121256-11590124-86078342 20303-20305 11590124 denotes 16
16121256-2919115-86078343 20306-20308 2919115 denotes 22
16121256-1997203-86078344 20624-20626 1997203 denotes 23
16121256-7829081-86078345 21071-21073 7829081 denotes 24
16121256-8666240-86078346 21074-21076 8666240 denotes 25
16121256-6380756-86078347 22065-22067 6380756 denotes 26
16121256-2440339-86078348 23189-23191 2440339 denotes 27
16121256-7640268-86078349 26458-26460 7640268 denotes 29
16121256-2919115-86078350 27262-27264 2919115 denotes 22
16121256-3367620-86078351 27265-27267 3367620 denotes 30
16121256-7494654-86078352 27574-27576 7494654 denotes 31
T84459 2647-2648 11826276 denotes 1
T69587 2763-2764 3611054 denotes 2
T60564 2763-2764 3035565 denotes 2
T12676 2763-2764 3813556 denotes 2
T23276 2886-2887 3813556 denotes 4
T83102 3081-3082 7172449 denotes 5
T12275 3131-3132 11826276 denotes 1
T87677 3133-3134 1438385 denotes 8
T56847 3133-3134 1601002 denotes 8
T46683 3307-3308 11826276 denotes 1
T65133 3309-3310 1601002 denotes 9
T97050 3533-3534 11826276 denotes 1
T9545 3678-3679 11826276 denotes 1
T75786 4180-4182 2660260 denotes 13
T37751 4183-4185 1591000 denotes 14
T51581 16568-16570 3054550 denotes 15
T54031 17027-17029 11590124 denotes 16
T82680 18063-18064 11826276 denotes 1
T5881 19056-19058 12897989 denotes 17
T84974 19060-19062 15858970 denotes 18
T76819 19134-19136 9498103 denotes 19
T6939 19229-19230 11826276 denotes 1
T95865 19543-19544 11826276 denotes 1
T70433 19545-19547 3786030 denotes 20
T33523 19804-19806 11590124 denotes 16
T5628 19807-19809 9861014 denotes 21
T94082 19810-19812 2919115 denotes 22
T78823 20024-20026 9861014 denotes 21
T19746 20303-20305 11590124 denotes 16
T30516 20306-20308 2919115 denotes 22
T4499 20624-20626 1997203 denotes 23
T57310 21071-21073 7829081 denotes 24
T81312 21074-21076 8666240 denotes 25
T40238 22065-22067 6380756 denotes 26
T68645 23189-23191 2440339 denotes 27
T69667 26458-26460 7640268 denotes 29
T84916 27262-27264 2919115 denotes 22
T17032 27265-27267 3367620 denotes 30
T91334 27574-27576 7494654 denotes 31

craft-ca-core-ex-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T262 0-21 CHEBI:61907 denotes Medium-Chain Acyl-CoA
T263 0-35 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes Medium-Chain Acyl-CoA Dehydrogenase
T264 50-54 SO_EXT:0000704 denotes Gene
T265 64-68 NCBITaxon:10088 denotes Mice
T266 69-134 CHEBI:61907 denotes MCAD Gene Disruption in the Mouse Abstract Medium-chain acyl-CoA
T267 69-148 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD Gene Disruption in the Mouse Abstract Medium-chain acyl-CoA dehydrogenase
T268 150-154 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T269 208-221 GO:0005739 denotes mitochondrial
T270 222-227 CHEBI_UBERON_EXT:triglyceride_or_adipose_tissue denotes fatty
T271 222-232 CHEBI:35366 denotes fatty acid
T272 222-244 GO:0006635 denotes fatty acid β-oxidation
T273 235-244 MOP:0000568 denotes oxidation
T274 248-254 NCBITaxon:9606 denotes humans
T275 326-331 NCBITaxon:10088 denotes mouse
T276 342-346 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T277 359-363 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T278 363-364 SO_EXT:sequence_nullness_or_absence denotes −
T279 365-366 SO_EXT:sequence_nullness_or_absence denotes −
T280 371-375 SO_EXT:0000704 denotes gene
T281 389-398 UBERON:0000922 denotes embryonic
T282 389-403 _FRAGMENT denotes embryonic stem
T283 409-414 CL:0002322 denotes cells
T284 405-407 _FRAGMENT denotes ES
T285 409-414 CL_GO_EXT:cell denotes cells
T286 420-424 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T287 424-425 SO_EXT:sequence_nullness_or_absence denotes −
T288 426-427 SO_EXT:sequence_nullness_or_absence denotes −
T289 428-432 NCBITaxon:10088 denotes mice
T290 467-472 CHEBI_UBERON_EXT:triglyceride_or_adipose_tissue denotes fatty
T291 473-478 UBERON:0002107 denotes liver
T292 558-565 UBERON:0000948 denotes cardiac
T293 582-586 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T294 586-587 SO_EXT:sequence_nullness_or_absence denotes −
T295 588-589 SO_EXT:sequence_nullness_or_absence denotes −
T296 590-594 NCBITaxon:10088 denotes mice
T297 621-626 NCBITaxon:9606 denotes human
T298 627-631 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T299 664-672 PATO_UBERON_EXT:neonate_or_newborn denotes neonatal
T300 686-690 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T301 690-691 SO_EXT:sequence_nullness_or_absence denotes −
T302 692-693 SO_EXT:sequence_nullness_or_absence denotes −
T303 694-698 NCBITaxon_UBERON_EXT:pup denotes pups
T304 776-780 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T305 805-809 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T306 820-825 NCBITaxon:10088 denotes mouse
T307 858-863 NCBITaxon:9606 denotes human
T308 864-868 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T309 941-946 CHEBI_UBERON_EXT:triglyceride_or_adipose_tissue denotes fatty
T310 941-951 CHEBI:35366 denotes fatty acid
T311 941-961 GO:0019395 denotes fatty acid oxidation
T312 952-961 MOP:0000568 denotes oxidation
T313 982-987 NCBITaxon:9606 denotes human
T314 1007-1012 CHEBI_UBERON_EXT:triglyceride_or_adipose_tissue denotes fatty
T315 1007-1017 CHEBI:35366 denotes fatty acid
T316 1007-1027 GO:0019395 denotes fatty acid oxidation
T317 1018-1027 MOP:0000568 denotes oxidation
T839 2143-2156 GO:0005739 denotes Mitochondrial
T840 2157-2183 GO:0006635 denotes β-oxidation of fatty acids
T841 2159-2168 MOP:0000568 denotes oxidation
T842 2172-2177 CHEBI_UBERON_EXT:triglyceride_or_adipose_tissue denotes fatty
T843 2172-2183 CHEBI:35366 denotes fatty acids
T844 2239-2244 CHEBI_UBERON_EXT:triglyceride_or_adipose_tissue denotes Fatty
T845 2239-2249 CHEBI:35366 denotes Fatty acid
T846 2239-2259 GO:0019395 denotes Fatty acid oxidation
T847 2250-2259 MOP:0000568 denotes oxidation
T848 2270-2282 GO:0005739 denotes mitochondria
T849 2375-2383 CHEBI:17984 denotes acyl-CoA
T850 2375-2398 GO_EXT:0003995 denotes acyl-CoA dehydrogenases
T851 2429-2450 CHEBI:61907 denotes Medium-chain acyl-CoA
T852 2429-2464 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes Medium-chain acyl-CoA dehydrogenase
T853 2466-2470 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T854 2477-2482 NCBITaxon:10088 denotes mouse
T855 2483-2487 SO_EXT:0000704 denotes gene
T856 2491-2496 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes Acadm
T857 2510-2517 CHEBI_PR_EXT:protein denotes protein
T858 2521-2525 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T859 2561-2571 GO_MOP_EXT:catalysis denotes catalyzing
T860 2576-2591 MOP:0000590 denotes dehydrogenation
T861 2595-2614 _FRAGMENT denotes medium-chain length
T862 2624-2634 CHEBI:59554 denotes fatty acid
T863 2624-2629 CHEBI_UBERON_EXT:triglyceride_or_adipose_tissue denotes fatty
T864 2635-2645 CHEBI:51277 denotes thioesters
T865 2651-2656 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes Acadm
T866 2660-2671 GO_EXT:transcription denotes transcribed
T867 2679-2686 GO:0005634 denotes nucleus
T868 2688-2698 GO:0006412 denotes translated
T869 2706-2713 GO:0005829 denotes cytosol
T870 2719-2731 GO_EXT:biological_movement_or_translocation_process denotes translocated
T871 2741-2761 GO:0005759 denotes mitochondrial matrix
T872 2785-2805 GO:0005759 denotes mitochondrial matrix
T873 2811-2815 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T874 2848-2857 GO_EXT:tetrameric_macromolecular_complex denotes tetramers
T875 2866-2875 CHEBI_GO_SO_EXT:enzyme denotes enzymatic
T876 2890-2894 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T877 2930-2935 CHEBI_UBERON_EXT:triglyceride_or_adipose_tissue denotes fatty
T878 2930-2940 CHEBI:35366 denotes fatty acid
T879 2930-2950 GO:0019395 denotes fatty acid oxidation
T880 2941-2950 MOP:0000568 denotes oxidation
T881 2962-2966 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T882 2988-2994 NCBITaxon:9606 denotes humans
T883 3001-3010 GO:0030849 denotes autosomal
T884 3031-3035 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T885 3316-3320 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T886 3461-3465 UBERON:0000104 denotes life
T887 3637-3640 GO:0016265 denotes die
T888 3720-3729 GO:0008152 denotes metabolic
T889 3746-3750 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T1482 4407-4416 SO_EXT:sequence_insertion_entity_or_process denotes insertion
T1484 4425-4429 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T1485 4459-4469 GO_EXT:DNA_gap_repair denotes gap repair
T1486 4482-4483 CHEBI_SO_EXT:base denotes b
T1487 4484-4491 SO_EXT:sequence_deletion_process denotes deleted
T1488 4515-4528 GO_SO_EXT:sequence_rearrangement_process denotes recombination
T1489 4553-4561 CL:0002322 denotes ES cells
T1490 4556-4561 CL_GO_EXT:cell denotes cells
T1491 4594-4598 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T1492 4619-4630 SO_EXT:sequence_duplication_entity_or_process denotes duplication
T1493 4634-4639 SO_EXT:0000147 denotes exons
T1494 4672-4680 SO:0000357 denotes flanking
T1495 4681-4688 SO_EXT:0000155 denotes plasmid
T1496 4693-4696 CHEBI_GO_EXT:neomycin_or_neomycin_phosphotransferase denotes Neo
T1497 4697-4706 SO_EXT:biological_sequence denotes sequences
T1498 4724-4733 SO_EXT:sequence_insertion_entity_or_process denotes insertion
T1499 4734-4740 SO_EXT:0000440 denotes vector
T1500 4757-4766 SO_EXT:sequence_duplication_process denotes duplicate
T1501 4767-4771 SO_EXT:0000147 denotes exon
T1502 4792-4796 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T1503 4804-4815 GO:0006412 denotes Translation
T1504 4823-4833 SO_EXT:sequence_duplication_process denotes duplicated
T1505 4834-4838 SO_EXT:0000147 denotes exon
T1506 4876-4897 SO_EXT:0001587 denotes premature stop codons
T1507 4911-4921 SO_EXT:sequence_truncation_entity_or_process denotes truncation
T1508 4929-4933 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T1509 4967-4987 SO_EXT:0001587 denotes premature stop codon
T1510 5001-5012 GO:0006412 denotes translation
T1511 5027-5038 CHEBI_SO_EXT:amino_acid denotes amino acids
T1512 5048-5058 SO_EXT:sequence_duplication_process denotes duplicated
T1513 5059-5063 SO_EXT:0000147 denotes exon
T1514 5081-5085 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T1515 5109-5119 CHEBI_SO_EXT:C_terminus_or_C_terminal_region denotes C-terminal
T1516 5120-5126 SO_EXT:0000417 denotes domain
T1517 5127-5136 SO_EXT:0001117 denotes α-helixes
T1518 5218-5222 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T1519 5227-5235 GO_EXT:tetrameric_macromolecular_complex denotes tetramer
T1534 6323-6327 CL_GO_EXT:cell denotes cell
T1535 6328-6334 SO_EXT:sequence_cloned_entity denotes clones
T1536 6409-6413 NCBITaxon:10088 denotes mice
T1537 6424-6428 NCBITaxon:10088 denotes mice
T1538 6474-6478 NCBITaxon:10088 denotes mice
T1539 6490-6494 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T1540 6494-6495 SO_EXT:normal_or_wild_type_or_present denotes +
T1541 6496-6497 SO_EXT:sequence_nullness_or_absence denotes −
T1542 6513-6517 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T1543 6517-6518 SO_EXT:sequence_nullness_or_absence denotes −
T1544 6519-6520 SO_EXT:sequence_nullness_or_absence denotes −
T1545 6521-6525 NCBITaxon:10088 denotes mice
T1546 6739-6746 SO_EXT:sequence_altered_entity denotes mutants
T1547 6766-6774 SO_EXT:sequence_alteration_entity_or_process denotes mutation
T1548 6789-6795 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T1549 6855-6859 NCBITaxon:10088 denotes mice
T1550 6870-6878 GO_EXT:breeder denotes breeders
T1551 6882-6892 SO_EXT:wild_type_entity_or_quality denotes wild-types
T1552 6919-6924 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes Acadm
T1553 6925-6933 SO_EXT:sequence_alteration_entity_or_process denotes mutation
T1554 6997-7005 PATO_UBERON_EXT:neonate_or_newborn denotes neonatal
T1555 7006-7011 GO:0016265 denotes death
T1556 7028-7037 CHEBI_EXT:polyatomic_entity_or_group denotes molecular
T1557 7064-7070 SO_EXT:0001023 denotes allele
T1558 7138-7147 SO_EXT:genotype_or_entity_with_genotype denotes genotypes
T1559 7213-7219 SO_EXT:0001023 denotes allele
T1560 7251-7256 NCBITaxon:10088 denotes mouse
T1561 7261-7265 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T1562 7265-7266 SO_EXT:sequence_nullness_or_absence denotes −
T1563 7267-7268 SO_EXT:sequence_nullness_or_absence denotes −
T1564 7272-7276 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T1565 7276-7277 SO_EXT:normal_or_wild_type_or_present denotes +
T1566 7278-7279 SO_EXT:sequence_nullness_or_absence denotes −
T1567 7331-7342 SO_EXT:genomic_DNA denotes genomic DNA
T1568 7339-7342 CHEBI_SO_EXT:DNA denotes DNA
T1569 7355-7359 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T1570 7359-7360 SO_EXT:sequence_nullness_or_absence denotes −
T1571 7361-7362 SO_EXT:sequence_nullness_or_absence denotes −
T1572 7363-7367 NCBITaxon:10088 denotes mice
T1573 7453-7457 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T1574 7457-7458 SO_EXT:sequence_nullness_or_absence denotes −
T1575 7459-7460 SO_EXT:sequence_nullness_or_absence denotes −
T1576 7465-7469 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T1577 7469-7470 SO_EXT:normal_or_wild_type_or_present denotes +
T1578 7471-7472 SO_EXT:normal_or_wild_type_or_present denotes +
T1579 7473-7477 NCBITaxon:10088 denotes mice
T1859 7499-7502 CHEBI_SO_EXT:RNA denotes RNA
T1860 7512-7514 GO_EXT:reverse_transcription_or_reverse_transcriptase denotes RT
T1861 7538-7542 SO_EXT:0000147 denotes exon
T1862 7562-7567 UBERON:0000948 denotes heart
T1863 7568-7571 CHEBI_SO_EXT:RNA denotes RNA
T1864 7599-7603 CHEBI_SO_EXT:base denotes base
T1865 7599-7608 SO_EXT:0000028 denotes base pair
T1866 7610-7612 SO_EXT:0000028 denotes bp
T1867 7626-7630 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T1868 7630-7631 SO_EXT:normal_or_wild_type_or_present denotes +
T1869 7632-7633 SO_EXT:normal_or_wild_type_or_present denotes +
T1870 7638-7642 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T1871 7642-7643 SO_EXT:normal_or_wild_type_or_present denotes +
T1872 7644-7645 SO_EXT:sequence_nullness_or_absence denotes −
T1873 7646-7650 NCBITaxon:10088 denotes mice
T1874 7663-7664 CHEBI_SO_EXT:base denotes b
T1875 7677-7681 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T1876 7681-7682 SO_EXT:sequence_nullness_or_absence denotes −
T1877 7683-7684 SO_EXT:sequence_nullness_or_absence denotes −
T1878 7685-7689 NCBITaxon:10088 denotes mice
T1879 7708-7716 SO_EXT:biological_sequence denotes Sequence
T1880 7738-7739 CHEBI_SO_EXT:base denotes b
T1881 7799-7803 SO_EXT:0000147 denotes exon
T1882 7809-7813 SO_EXT:0000147 denotes exon
T1883 7826-7828 SO_EXT:0000028 denotes bp
T1884 7837-7844 SO_EXT:0000155 denotes plasmid
T1885 7845-7853 SO_EXT:biological_sequence denotes sequence
T1886 7866-7871 SO_EXT:0000147 denotes exons
T1887 7890-7897 SO_EXT:0000155 denotes plasmid
T1888 7898-7907 SO_EXT:biological_sequence denotes sequences
T1889 7934-7942 SO_EXT:biological_sequence denotes sequence
T1890 7949-7956 SO_EXT:sequence_deletion_process denotes deleted
T1891 7967-7974 GO:0008380 denotes spliced
T1892 7975-7979 CHEBI_SO_EXT:mRNA denotes mRNA
T1893 8013-8018 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes Acadm
T1894 8032-8041 GO:0010467 denotes expressed
T1895 8049-8056 UBERON:0000479 denotes tissues
T1896 8071-8075 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T1897 8075-8076 SO_EXT:normal_or_wild_type_or_present denotes +
T1898 8077-8078 SO_EXT:normal_or_wild_type_or_present denotes +
T1899 8079-8083 NCBITaxon:10088 denotes mice
T1900 8105-8115 GO:0010467 denotes expression
T1901 8129-8138 UBERON:0001348 denotes brown fat
T1902 8135-8138 CHEBI_UBERON_EXT:triglyceride_or_adipose_tissue denotes fat
T1903 8140-8146 UBERON:0002113 denotes kidney
T1904 8148-8153 UBERON:0000948 denotes heart
T1905 8155-8170 UBERON_EXT:skeletal_muscle_organ_or_tissue denotes skeletal muscle
T1906 8176-8181 UBERON:0002107 denotes liver
T1907 8195-8205 GO:0010467 denotes expression
T1908 8213-8218 UBERON:0000955 denotes brain
T1909 8220-8229 UBERON:0001347 denotes white fat
T1910 8226-8229 CHEBI_UBERON_EXT:triglyceride_or_adipose_tissue denotes fat
T1911 8235-8241 UBERON:0000473 denotes testes
T1912 8278-8280 GO_EXT:reverse_transcription_or_reverse_transcriptase denotes RT
T1913 8310-8317 GO:0008380 denotes spliced
T1914 8310-8317 _FRAGMENT denotes spliced
T1915 8324-8334 SO_EXT:1001187 denotes transcript
T1916 8318-8323 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes Acadm
T1917 8339-8344 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes Acadm
T1918 8345-8356 SO_EXT:0000673 denotes transcripts
T1919 8402-8406 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T1920 8406-8407 SO_EXT:sequence_nullness_or_absence denotes −
T1921 8408-8409 SO_EXT:sequence_nullness_or_absence denotes −
T1922 8410-8414 NCBITaxon:10088 denotes mice
T1923 8456-8462 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T1924 8463-8466 CHEBI_SO_EXT:RNA denotes RNA
T1925 8529-8537 SO_EXT:nonsense_quality_or_entity denotes nonsense
T1926 8529-8556 GO:0000184 denotes nonsense mediated RNA decay
T1927 8547-8550 CHEBI_SO_EXT:RNA denotes RNA
T2210 8936-8941 UBERON:0002107 denotes Liver
T2211 8942-8948 CHEBI_GO_SO_EXT:enzyme denotes Enzyme
T2212 8981-8986 UBERON:0002107 denotes liver
T2213 9009-9013 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T2214 9018-9022 UBERON:0001977 denotes sera
T2215 9052-9056 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T2216 9080-9084 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T2254 10113-10125 CHEBI:15533 denotes octanoyl-CoA
T2255 10168-10181 CHEBI:15525 denotes palmitoyl-CoA
T2256 10194-10198 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T2257 10198-10199 SO_EXT:normal_or_wild_type_or_present denotes +
T2258 10200-10201 SO_EXT:normal_or_wild_type_or_present denotes +
T2259 10202-10206 NCBITaxon:10088 denotes mice
T2260 10236-10251 MOP:0000590 denotes dehydrogenation
T2261 10255-10267 CHEBI:15533 denotes octanoyl-CoA
T2262 10272-10285 CHEBI:15525 denotes palmitoyl-CoA
T2263 10346-10350 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T2264 10350-10351 SO_EXT:sequence_nullness_or_absence denotes −
T2265 10352-10353 SO_EXT:sequence_nullness_or_absence denotes −
T2266 10354-10358 NCBITaxon:10088 denotes mice
T2267 10374-10378 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T2268 10378-10379 SO_EXT:normal_or_wild_type_or_present denotes +
T2269 10380-10381 SO_EXT:normal_or_wild_type_or_present denotes +
T7411 5278-5283 NCBITaxon:10088 denotes Mouse
T7412 5284-5289 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes Acadm
T7413 5290-5294 SO_EXT:0000704 denotes Gene
T7414 5303-5307 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T7415 5312-5321 SO_EXT:sequence_insertion_entity_or_process denotes insertion
T7416 5322-5338 SO_EXT:0001644 denotes targeting vector
T7417 5346-5353 SO_EXT:sequence_deletion_process denotes deleted
T7418 5359-5360 CHEBI_SO_EXT:base denotes b
T7419 5381-5385 SO_EXT:0000147 denotes exon
T7420 5393-5401 SO:0000357 denotes flanking
T7421 5402-5411 SO_EXT:biological_sequence denotes sequences
T7422 5413-5417 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T7423 5432-5442 GO_EXT:DNA_gap_repair denotes gap repair
T7424 5459-5472 GO_SO_EXT:sequence_rearrangement_process denotes recombination
T7425 5491-5496 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes Acadm
T7426 5518-5529 SO_EXT:sequence_duplication_entity_or_process denotes duplication
T7427 5533-5538 SO_EXT:0000147 denotes exons
T7428 5569-5575 SO_EXT:0001023 denotes allele
T7429 5622-5633 SO_EXT:genomic_DNA denotes genomic DNA
T7430 5630-5633 CHEBI_SO_EXT:DNA denotes DNA
T7431 5639-5647 CL:0002322 denotes ES cells
T7432 5642-5647 CL_GO_EXT:cell denotes cells
T7433 5665-5670 CHEBI_SO_EXT:molecular_probe denotes Probe
T7434 5676-5679 CHEBI_SO_EXT:DNA denotes DNA
T7435 5705-5720 SO_EXT:0000852 denotes portion of exon
T7436 5751-5767 SO_EXT:0001644 denotes targeting vector
T7437 5769-5779 GO:0097617 denotes hybridizes
T7438 5802-5803 CHEBI_SO_EXT:base denotes b
T7439 5834-5847 GO_SO_EXT:sequence_rearrangement_process denotes recombination
T7440 5860-5861 CHEBI_SO_EXT:base denotes b
T7441 5892-5901 SO_EXT:wild_type_entity_or_quality denotes wild-type
T7442 5902-5909 CL:0002322 denotes ES cell
T7443 5905-5909 CL_GO_EXT:cell denotes cell
T7444 5952-5959 CL:0002322 denotes ES cell
T7445 5955-5959 CL_GO_EXT:cell denotes cell
T7570 8596-8600 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T7571 8600-8601 SO_EXT:sequence_nullness_or_absence denotes −
T7572 8602-8603 SO_EXT:sequence_nullness_or_absence denotes −
T7573 8616-8620 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T7574 8620-8621 SO_EXT:normal_or_wild_type_or_present denotes +
T7575 8622-8623 SO_EXT:normal_or_wild_type_or_present denotes +
T7576 8632-8636 NCBITaxon:10088 denotes Mice
T7577 8637-8642 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes Acadm
T7578 8643-8650 SO_EXT:0000673 denotes message
T7579 8643-8650 CHEBI:33695 denotes message
T7580 8673-8678 UBERON:0000948 denotes heart
T7581 8680-8685 UBERON:0002107 denotes liver
T7582 8687-8696 UBERON:0001348 denotes brown fat
T7583 8693-8696 CHEBI_UBERON_EXT:triglyceride_or_adipose_tissue denotes fat
T7584 8698-8703 UBERON:0000955 denotes brain
T7585 8705-8711 UBERON:0002113 denotes kidney
T7586 8717-8723 UBERON_EXT:muscle_structure_or_tissue denotes muscle
T7587 8729-8738 UBERON:0001347 denotes white fat
T7588 8735-8738 CHEBI_UBERON_EXT:triglyceride_or_adipose_tissue denotes fat
T7589 8743-8749 UBERON:0000473 denotes testes
T7590 8775-8779 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T7591 8779-8780 SO_EXT:normal_or_wild_type_or_present denotes +
T7592 8781-8782 SO_EXT:normal_or_wild_type_or_present denotes +
T7593 8783-8787 NCBITaxon:10088 denotes mice
T7594 8801-8811 GO:0010467 denotes expression
T7595 8824-8833 UBERON:0001348 denotes brown fat
T7596 8830-8833 CHEBI_UBERON_EXT:triglyceride_or_adipose_tissue denotes fat
T7597 8835-8841 UBERON:0002113 denotes kidney
T7598 8843-8848 UBERON:0000948 denotes heart
T7599 8854-8869 UBERON_EXT:skeletal_muscle_organ_or_tissue denotes skeletal muscle
T7600 8871-8875 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T7601 8875-8876 SO_EXT:sequence_nullness_or_absence denotes −
T7602 8877-8878 SO_EXT:sequence_nullness_or_absence denotes −
T7603 8879-8883 NCBITaxon:10088 denotes mice
T7604 8902-8909 SO_EXT:0000673 denotes message
T7605 8902-8909 CHEBI:33695 denotes message
T7606 8917-8924 UBERON:0000479 denotes tissues
T7759 9343-9348 UBERON:0002107 denotes Liver
T7760 9366-9370 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T7761 9370-9371 SO_EXT:normal_or_wild_type_or_present denotes +
T7762 9372-9373 SO_EXT:normal_or_wild_type_or_present denotes +
T7763 9378-9382 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T7764 9382-9383 SO_EXT:sequence_nullness_or_absence denotes −
T7765 9384-9385 SO_EXT:sequence_nullness_or_absence denotes −
T7766 9386-9390 NCBITaxon:10088 denotes Mice
T7767 9419-9423 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T7768 9424-9432 GO:0042571 denotes antibody
T7769 9441-9445 GO_PR_EXT:short_chain_acyl_CoA_dehydrogenase denotes SCAD
T7770 9446-9454 GO:0042571 denotes antibody
T7771 9467-9471 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T7772 9471-9472 SO_EXT:sequence_nullness_or_absence denotes −
T7773 9473-9474 SO_EXT:sequence_nullness_or_absence denotes −
T7774 9475-9479 NCBITaxon:10088 denotes mice
T7775 9498-9502 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T7776 9503-9510 CHEBI_PR_EXT:protein denotes protein
T7777 9512-9516 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T7778 9517-9524 CHEBI_PR_EXT:protein denotes protein
T7779 9554-9558 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T7780 9559-9566 CHEBI_PR_EXT:protein denotes protein
T7781 9622-9627 UBERON:0002107 denotes liver
T7782 9657-9661 GO_PR_EXT:short_chain_acyl_CoA_dehydrogenase denotes SCAD
T7783 9662-9670 GO:0042571 denotes antibody
T7784 9685-9689 GO_PR_EXT:short_chain_acyl_CoA_dehydrogenase denotes SCAD
T7785 9690-9697 CHEBI_PR_EXT:protein denotes protein
T7786 9718-9722 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T7787 9722-9723 SO_EXT:normal_or_wild_type_or_present denotes +
T7788 9724-9725 SO_EXT:normal_or_wild_type_or_present denotes +
T7789 9730-9734 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T7790 9734-9735 SO_EXT:sequence_nullness_or_absence denotes −
T7791 9736-9737 SO_EXT:sequence_nullness_or_absence denotes −
T7792 9738-9742 NCBITaxon:10088 denotes mice
T7793 9747-9751 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T7794 9769-9776 CHEBI_PR_EXT:protein denotes protein
T7795 9797-9801 GO_PR_EXT:short_chain_acyl_CoA_dehydrogenase denotes SCAD
T7796 9802-9812 GO:0042571 denotes antibodies
T7797 9814-9818 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T7798 9830-9839 CHEBI_EXT:polyatomic_entity_or_group denotes molecular
T8170 10420-10424 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T8171 10435-10439 NCBITaxon:10088 denotes Mice
T8160 15438-15442 NCBITaxon:10088 denotes mice
T8159 15436-15437 SO_EXT:sequence_nullness_or_absence denotes −
T8158 15434-15435 SO_EXT:sequence_nullness_or_absence denotes −
T8157 15430-15434 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T8156 15418-15426 GO_PATO_EXT:necrosis denotes necrosis
T8155 15393-15400 CL:0000187 denotes myocyte
T8154 15323-15327 NCBITaxon:10088 denotes mice
T8153 15321-15322 SO_EXT:sequence_nullness_or_absence denotes −
T8152 15319-15320 SO_EXT:sequence_nullness_or_absence denotes −
T8151 15315-15319 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T8150 15294-15301 UBERON:0002107 denotes hepatic
T8149 15284-15293 GO:0031982 denotes vesicular
T8148 15265-15274 GO:0031982 denotes vesicular
T8147 15217-15222 UBERON:0002107 denotes liver
T8146 15170-15175 NCBITaxon:10088 denotes mouse
T8145 15168-15169 SO_EXT:sequence_nullness_or_absence denotes −
T8144 15166-15167 SO_EXT:sequence_nullness_or_absence denotes −
T8143 15162-15166 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T8142 15103-15108 UBERON:0002107 denotes Liver
T8141 15062-15069 UBERON:0002107 denotes hepatic
T8140 15038-15042 NCBITaxon:10088 denotes mice
T8139 15036-15037 SO_EXT:normal_or_wild_type_or_present denotes +
T8138 15034-15035 SO_EXT:normal_or_wild_type_or_present denotes +
T8137 15030-15034 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T8136 15021-15025 NCBITaxon:10088 denotes Mice
T8135 15019-15020 SO_EXT:sequence_nullness_or_absence denotes −
T8134 15017-15018 SO_EXT:sequence_nullness_or_absence denotes −
T8133 15013-15017 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T8132 15007-15008 SO_EXT:normal_or_wild_type_or_present denotes +
T8131 15005-15006 SO_EXT:normal_or_wild_type_or_present denotes +
T8130 15001-15005 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T7996 14341-14345 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T7995 14322-14327 NCBITaxon:9606 denotes human
T7994 14302-14307 NCBITaxon:10088 denotes mouse
T7993 14300-14301 SO_EXT:sequence_nullness_or_absence denotes −
T7992 14298-14299 SO_EXT:sequence_nullness_or_absence denotes −
T7991 14294-14298 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T7990 14266-14279 CHEBI:17387 denotes acylcarnitine
T7989 14261-14265 UBERON:0001970 denotes Bile
T7988 14160-14164 NCBITaxon:10088 denotes mice
T7987 14133-14147 CHEBI:17387 denotes acylcarnitines
T7986 14128-14132 UBERON:0001970 denotes bile
T7985 13971-13975 NCBITaxon:10088 denotes mice
T7984 13969-13970 SO_EXT:sequence_nullness_or_absence denotes −
T7983 13967-13968 SO_EXT:sequence_nullness_or_absence denotes −
T7982 13963-13967 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T7981 13925-13938 CHEBI:17387 denotes acylcarnitine
T7980 13876-13880 NCBITaxon:10088 denotes mice
T7979 13874-13875 SO_EXT:sequence_nullness_or_absence denotes −
T7978 13872-13873 SO_EXT:sequence_nullness_or_absence denotes −
T7977 13868-13872 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T7976 13854-13855 SO_EXT:normal_or_wild_type_or_present denotes +
T7975 13852-13853 SO_EXT:normal_or_wild_type_or_present denotes +
T7974 13848-13852 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T7973 13822-13835 CHEBI:17387 denotes acylcarnitine
T7972 13816-13821 UBERON:0001977 denotes Serum
T7971 13789-13802 CHEBI:17387 denotes Acylcarnitine
T7200 28392-28399 UBERON:0000948 denotes cardiac
T7199 28353-28360 UBERON:0000948 denotes cardiac
T7198 28277-28281 NCBITaxon:10088 denotes mice
T7197 28254-28262 SO_EXT:genotype_or_entity_with_genotype denotes genotype
T7196 28118-28123 UBERON:0002107 denotes liver
T7195 28104-28109 CHEBI_EXT:eosin denotes eosin
T7194 28088-28099 CHEBI:51686 denotes hematoxylin
T7193 27997-28005 CHEBI:16842 denotes formalin
T7192 27984-27992 CHEBI_CHMO_EXT:buffer_process denotes buffered
T7191 27941-27956 UBERON_EXT:skeletal_muscle_organ_or_tissue denotes skeletal muscle
T7190 27928-27935 UBERON:0000992 denotes ovaries
T7189 27917-27926 UBERON:0000473 denotes testicles
T7188 27910-27915 UBERON:0000948 denotes heart
T7187 27903-27908 UBERON:0000955 denotes brain
T7186 27896-27901 UBERON:0002107 denotes liver
T7185 27886-27894 UBERON:0001264 denotes pancreas
T7184 27878-27884 UBERON:0002106 denotes spleen
T7183 27870-27876 UBERON:0002113 denotes Kidney
T7182 27855-27859 NCBITaxon:10088 denotes mice
T7181 27853-27854 SO_EXT:normal_or_wild_type_or_present denotes +
T7180 27851-27852 SO_EXT:normal_or_wild_type_or_present denotes +
T7179 27847-27851 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T7178 27840-27846 PATO_UBERON_EXT:female_or_bearer_of_femaleness denotes female
T7177 27827-27831 PATO_UBERON_EXT:male_or_bearer_of_maleness denotes male
T7176 27804-27808 NCBITaxon:10088 denotes mice
T7175 27802-27803 SO_EXT:sequence_nullness_or_absence denotes −
T7174 27800-27801 SO_EXT:sequence_nullness_or_absence denotes −
T7173 27796-27800 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T7172 27789-27795 PATO_UBERON_EXT:female_or_bearer_of_femaleness denotes female
T7171 27776-27780 PATO_UBERON_EXT:male_or_bearer_of_maleness denotes male
T7170 27756-27761 NCBITaxon:10088 denotes mouse
T7169 27754-27755 SO_EXT:normal_or_wild_type_or_present denotes +
T7168 27752-27753 SO_EXT:normal_or_wild_type_or_present denotes +
T7167 27748-27752 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T7166 27741-27747 PATO_UBERON_EXT:female_or_bearer_of_femaleness denotes female
T7165 27728-27732 PATO_UBERON_EXT:male_or_bearer_of_maleness denotes male
T7164 27707-27712 NCBITaxon:10088 denotes mouse
T7163 27705-27706 SO_EXT:sequence_nullness_or_absence denotes −
T7162 27703-27704 SO_EXT:sequence_nullness_or_absence denotes −
T7161 27699-27703 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T7160 27692-27698 PATO_UBERON_EXT:female_or_bearer_of_femaleness denotes female
T7159 27679-27683 PATO_UBERON_EXT:male_or_bearer_of_maleness denotes male
T7158 27606-27610 NCBITaxon:10088 denotes mice
T6946 27509-27513 UBERON:0001970 denotes bile
T6945 27499-27504 UBERON:0001977 denotes serum
T6944 27473-27486 CHEBI:17387 denotes Acylcarnitine
T6943 27277-27288 CHEBI:32936 denotes tetracosane
T6942 27157-27169 CHEBI:64709 denotes organic acid
T6941 27151-27156 UBERON:0001088 denotes Urine
T6940 26942-26950 CHEBI_EXT:33893 denotes reagents
T6939 26901-26910 CHEBI_GO_SO_EXT:enzyme denotes enzymatic
T6938 26862-26873 CHEBI:35366 denotes fatty acids
T6937 26725-26729 UBERON:0001977 denotes sera
T6936 26681-26688 CHEBI:17234 denotes Glucose
T6935 26639-26648 CHEBI:17126 denotes carnitine
T6934 26621-26633 CHEBI:64709 denotes organic acid
T6933 26609-26619 CHEBI:35366 denotes fatty acid
T6932 26595-26602 CHEBI:17234 denotes glucose
T6931 26589-26594 UBERON:0001977 denotes serum
T6930 26525-26529 NCBITaxon:10088 denotes mice
T6929 26523-26524 SO_EXT:sequence_nullness_or_absence denotes −
T6928 26521-26522 SO_EXT:sequence_nullness_or_absence denotes −
T6927 26517-26521 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T6926 26511-26512 SO_EXT:normal_or_wild_type_or_present denotes +
T6925 26509-26510 SO_EXT:normal_or_wild_type_or_present denotes +
T6924 26505-26509 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T6552 25954-25962 GO:0042571 denotes antibody
T6551 25933-25943 MOP:0000779 denotes conjugated
T6550 25925-25928 GO:0071735 denotes IgG
T6549 25919-25924 NCBITaxon:10088 denotes mouse
T6548 25880-25888 GO:0042571 denotes antibody
T6547 25875-25879 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T6546 25785-25793 CHEBI:53424 denotes Tween-20
T6545 25768-25774 CHEBI_EXT:saline_solution denotes saline
T6544 25759-25767 CHEBI_CHMO_EXT:buffer_process denotes buffered
T6543 25749-25758 CHEBI_EXT:phosphate denotes phosphate
T6542 25741-25745 UBERON:0001913 denotes milk
T6541 25737-25740 CHEBI_UBERON_EXT:triglyceride_or_adipose_tissue denotes fat
T6540 25624-25638 CHEBI:53325 denotes nitrocellulose
T6539 25587-25598 GO_EXT:biological_movement_or_translocation_process denotes transferred
T6538 25573-25576 CHEBI:8984 denotes SDS
T6537 25546-25555 GO_EXT:macromolecule_denaturation denotes denatured
T6536 25525-25532 CHEBI_PR_EXT:protein denotes Protein
T6535 25463-25470 CHEBI_PR_EXT:protein denotes protein
T6534 25401-25421 CHEBI:35607 denotes sodium orthovanadate
T6533 25359-25387 CHEBI:8102 denotes phenylmethylsulfonylfluoride
T6532 25260-25278 CHEBI_GO_EXT:peptidase_or_protease_or_proteinase_inhibitor denotes Protease Inhibitor
T6531 25238-25246 CHEBI:17754 denotes glycerol
T6530 25224-25227 CHEBI:8984 denotes SDS
T6529 25196-25215 CHEBI:9177 denotes sodium deoxycholate
T6528 25177-25189 CHEBI:78708 denotes Nonidet P-40
T6527 25158-25164 CHEBI_CHMO_EXT:buffer_solution denotes buffer
T6526 25144-25149 GO:0019835 denotes lysed
T6525 25117-25123 UBERON:0000479 denotes tissue
T6524 25072-25080 CHEBI_EXT:nitrogen denotes nitrogen
T6523 25033-25037 NCBITaxon:10088 denotes mice
T6522 25023-25024 SO_EXT:sequence_nullness_or_absence denotes −
T6521 25021-25022 SO_EXT:sequence_nullness_or_absence denotes −
T6520 25017-25021 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T6519 25003-25004 SO_EXT:normal_or_wild_type_or_present denotes +
T6518 25001-25002 SO_EXT:normal_or_wild_type_or_present denotes +
T6517 24997-25001 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T6516 24967-24972 UBERON:0002107 denotes liver
T6515 24959-24965 UBERON:0000479 denotes tissue
T6514 24953-24958 NCBITaxon:10088 denotes mouse
T6513 24942-24949 CHEBI_PR_EXT:protein denotes protein
T6512 24937-24941 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T6511 24900-24907 CHEBI_PR_EXT:protein denotes protein
T6510 24895-24899 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T6185 24858-24861 CHEBI_SO_EXT:RNA denotes RNA
T6184 24825-24841 CHEBI:4883 denotes ethidium bromide
T6183 24791-24803 CHEBI:16842 denotes formaldehyde
T6182 24783-24790 CHEBI:2511 denotes agarose
T6181 24612-24615 CHEBI:8984 denotes SDS
T6180 24602-24605 CHEBI_EXT:saline_sodium_citrate denotes SSC
T6179 24578-24581 CHEBI:8984 denotes SDS
T6178 24568-24571 CHEBI_EXT:saline_sodium_citrate denotes SSC
T6177 24523-24533 GO:0097617 denotes hybridized
T6176 24504-24507 CHEBI_SO_EXT:DNA denotes DNA
T6175 24498-24503 CL:0000019 denotes sperm
T6174 24482-24485 CHEBI:8984 denotes SDS
T6173 24470-24477 CHEBI_EXT:33893 denotes reagent
T6172 24439-24454 CHEBI:34674 denotes dextran sulfate
T6171 24424-24433 CHEBI:16397 denotes formamide
T6170 24415-24418 CHEBI_EXT:saline_sodium_citrate denotes SSC
T6668 26400-26413 CHEBI:15525 denotes palmitoyl-CoA
T6667 26378-26390 CHEBI:15533 denotes octanoyl-CoA
T6666 26357-26363 UBERON:0000479 denotes tissue
T6665 26329-26338 MOP:0000569 denotes reduction
T6664 26325-26328 GO:0045251 denotes ETF
T6663 26267-26271 NCBITaxon:10088 denotes mice
T6662 26265-26266 SO_EXT:sequence_nullness_or_absence denotes −
T6660 26259-26263 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T6659 26245-26246 SO_EXT:normal_or_wild_type_or_present denotes +
T6658 26243-26244 SO_EXT:normal_or_wild_type_or_present denotes +
T6169 24338-24352 GO:0097617 denotes Hybridizations
T6168 24300-24305 CHEBI_SO_EXT:molecular_probe denotes probe
T6167 24295-24299 SO_EXT:cDNA denotes cDNA
T6166 24289-24294 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes Acadm
T6165 24283-24288 NCBITaxon:10088 denotes mouse
T6164 24258-24270 CHEBI_EXT:radiolabel_process denotes radiolabeled
T6163 24254-24257 CHEBI:37972 denotes 32P
T6162 24238-24248 GO:0097617 denotes hybridized
T6161 24119-24133 CHEBI:53325 denotes nitrocellulose
T6160 24104-24115 GO_EXT:biological_movement_or_translocation_process denotes transferred
T6159 24086-24098 CHEBI:16842 denotes formaldehyde
T6158 24078-24085 CHEBI:2511 denotes agarose
T6157 24034-24037 CHEBI_SO_EXT:RNA denotes RNA
T6156 23909-23912 CHEBI_SO_EXT:RNA denotes RNA
T6155 23884-23888 NCBITaxon:10088 denotes mice
T6154 23882-23883 SO_EXT:sequence_nullness_or_absence denotes −
T6153 23880-23881 SO_EXT:sequence_nullness_or_absence denotes −
T6152 23876-23880 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T6151 23870-23871 SO_EXT:normal_or_wild_type_or_present denotes +
T6150 23868-23869 SO_EXT:normal_or_wild_type_or_present denotes +
T6149 23864-23868 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T6148 23845-23851 UBERON:0000473 denotes testes
T6147 23836-23839 CHEBI_UBERON_EXT:triglyceride_or_adipose_tissue denotes fat
T6146 23830-23839 UBERON:0001347 denotes white fat
T6145 23813-23828 UBERON_EXT:skeletal_muscle_organ_or_tissue denotes skeletal muscle
T6144 23805-23811 UBERON:0002113 denotes kidney
T6114 23099-23100 SO_EXT:normal_or_wild_type_or_present denotes +
T6113 23097-23098 SO_EXT:normal_or_wild_type_or_present denotes +
T6112 23093-23097 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T6111 23073-23078 UBERON:0000948 denotes heart
T6110 23047-23050 CHEBI_SO_EXT:RNA denotes RNA
T6109 23027-23030 CHEBI_SO_EXT:RNA denotes RNA
T6143 23798-23803 UBERON:0000955 denotes brain
T6142 23793-23796 CHEBI_UBERON_EXT:triglyceride_or_adipose_tissue denotes fat
T6141 23787-23796 UBERON:0001348 denotes brown fat
T6140 23780-23785 UBERON:0002107 denotes liver
T6139 23773-23778 UBERON:0000948 denotes heart
T6138 23751-23754 CHEBI_SO_EXT:RNA denotes RNA
T6137 23701-23705 NCBITaxon:10088 denotes mice
T6136 23699-23700 SO_EXT:sequence_nullness_or_absence denotes −
T6135 23697-23698 SO_EXT:sequence_nullness_or_absence denotes −
T6134 23693-23697 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T6133 23674-23683 GO:0010467 denotes expressed
T6132 23669-23673 CHEBI_SO_EXT:mRNA denotes mRNA
T6131 23663-23668 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes Acadm
T6130 23574-23580 SO_EXT:0000440 denotes vector
T6129 23437-23442 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes Acadm
T6128 23426-23430 SO_EXT:0000147 denotes exon
T6127 23415-23419 SO_EXT:0000147 denotes exon
T6126 23386-23402 CHEBI_SO_EXT:oligonucleotide denotes oligonucleotides
T6125 23243-23259 CHEBI_SO_EXT:oligonucleotide denotes oligonucleotides
T6124 23194-23215 GO:0001171 denotes Reverse transcription
T6123 23169-23180 CHEBI:18022 denotes thiocyanate
T6122 23157-23168 CHEBI:30087 denotes guanidinium
T6121 23123-23127 NCBITaxon:10088 denotes mice
T6120 23121-23122 SO_EXT:sequence_nullness_or_absence denotes −
T6119 23119-23120 SO_EXT:sequence_nullness_or_absence denotes −
T6118 23115-23119 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T6117 23108-23109 SO_EXT:sequence_nullness_or_absence denotes −
T6116 23106-23107 SO_EXT:normal_or_wild_type_or_present denotes +
T6115 23102-23106 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T5627 22780-22781 SO_EXT:sequence_nullness_or_absence denotes −
T5626 22778-22779 SO_EXT:sequence_nullness_or_absence denotes −
T5625 22774-22778 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T5624 22755-22760 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes Acadm
T5623 22737-22738 SO_EXT:sequence_nullness_or_absence denotes −
T5622 22735-22736 SO_EXT:normal_or_wild_type_or_present denotes +
T5621 22731-22735 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T5620 22728-22729 SO_EXT:normal_or_wild_type_or_present denotes +
T5619 22716-22721 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes Acadm
T5618 22711-22715 NCBITaxon:10088 denotes mice
T5617 22697-22701 SO_EXT:0000704 denotes gene
T5616 22681-22685 NCBITaxon:10088 denotes mice
T5615 22630-22634 NCBITaxon:10088 denotes mice
T5614 22615-22619 NCBITaxon:10088 denotes mice
T5613 22582-22593 UBERON:0000358 denotes blastocysts
T5612 22548-22554 SO_EXT:sequence_cloned_entity denotes clones
T5611 22543-22547 CL_GO_EXT:cell denotes cell
T5610 22540-22547 CL:0002322 denotes ES cell
T5609 22506-22519 GO_SO_EXT:sequence_rearrangement_process denotes recombination
T5608 22477-22480 CHEBI_SO_EXT:DNA denotes DNA
T5607 22469-22480 SO_EXT:genomic_DNA denotes genomic DNA
T5606 22467-22468 CHEBI_SO_EXT:base denotes b
T5605 22446-22455 GO:0097617 denotes hybridize
T5604 22419-22425 SO_EXT:0000440 denotes vector
T5603 22409-22418 SO_EXT:sequence_insertion_entity_or_process denotes insertion
T5602 22403-22408 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes Acadm
T5601 22368-22371 CHEBI_SO_EXT:DNA denotes DNA
T5600 22352-22358 SO_EXT:0000188 denotes intron
T5599 22341-22345 SO_EXT:0000147 denotes exon
T5598 22335-22340 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes Acadm
T5597 22304-22309 CHEBI_SO_EXT:molecular_probe denotes probe
T5596 22297-22302 CHEBI_SO_EXT:molecular_probe denotes probe
T5595 22294-22296 SO_EXT:0000028 denotes bp
T5594 22208-22210 CL:0002322 denotes ES
T5593 22193-22197 CHEBI:42768 denotes G418
T5592 22184-22187 CHEBI_SO_EXT:DNA denotes DNA
T5591 22176-22187 SO_EXT:genomic_DNA denotes Genomic DNA
T5590 22161-22174 GO_SO_EXT:sequence_rearrangement_process denotes recombination
T5589 22087-22100 GO_SO_EXT:sequence_rearrangement_process denotes recombination
T5588 22035-22063 GO:0006302 denotes double stranded–break repair
T5587 22035-22050 SO:0000985 denotes double stranded
T5586 22021-22029 SO_EXT:sequence_deletion_entity_or_process denotes deletion
T5585 22019-22020 CHEBI_SO_EXT:base denotes b
T5584 21996-22006 GO_EXT:DNA_gap_repair denotes gap repair
T5583 21965-21971 SO_EXT:0000440 denotes vector
T5582 21955-21964 SO_EXT:sequence_insertion_entity_or_process denotes insertion
T5581 21949-21954 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes Acadm
T5580 21924-21928 NCBITaxon:10088 denotes mice
T5579 21871-21876 CL_GO_EXT:cell denotes cells
T5578 21868-21876 CL:0002322 denotes ES cells
T5577 21828-21836 SO_EXT:sequence_deletion_entity_or_process denotes deletion
T5576 21811-21818 SO_EXT:0001026 denotes genomic
T5575 21809-21810 CHEBI_SO_EXT:base denotes b
T5574 21758-21768 SO_EXT:sequence_linearization denotes linearized
T5573 21747-21753 SO_EXT:0000440 denotes vector
T5572 21737-21746 SO_EXT:sequence_insertion_entity_or_process denotes insertion
T5571 21731-21736 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes Acadm
T5570 21721-21725 NCBITaxon:10088 denotes mice
T5569 21706-21710 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T5245 21682-21689 SO_EXT:sequence_deletion_process denotes deleted
T5244 21663-21667 SO_EXT:0000147 denotes exon
T5243 21661-21662 CHEBI_SO_EXT:base denotes b
T5241 21535-21541 SO_EXT:0000440 denotes vector
T5240 21522-21534 SO_EXT:sequence_circularization denotes circularized
T5239 21438-21454 CHEBI_SO_EXT:oligonucleotide denotes oligonucleotides
T5238 21417-21425 GO:0006266 denotes ligation
T5237 21401-21413 SO_EXT:sequence_circularization denotes circularized
T5236 21344-21351 SO_EXT:0001026 denotes genomic
T5235 21332-21337 PR_EXT:P23940 denotes BamHI
T5234 21330-21331 CHEBI_SO_EXT:base denotes b
T5233 21305-21311 SO_EXT:0000440 denotes vector
T5232 21281-21290 SO_EXT:biological_sequence denotes sequences
T5231 21274-21280 SO_EXT:0000188 denotes intron
T5230 21265-21273 SO:0000357 denotes flanking
T5229 21253-21257 SO_EXT:0000147 denotes exon
T5228 21225-21232 SO_EXT:0001026 denotes genomic
T5227 21213-21218 PR_EXT:P23940 denotes BamHI
T5226 21211-21212 CHEBI_SO_EXT:base denotes b
T5225 21178-21183 PR_EXT:P23940 denotes BamHI
T5224 21153-21159 SO_EXT:0000440 denotes vector
T5223 21097-21103 SO_EXT:sequence_cloning_process denotes cloned
T5222 21054-21061 SO_EXT:0001026 denotes genomic
T5221 21048-21053 NCBITaxon:10088 denotes mouse
T5220 20991-21000 SO_EXT:biological_sequence denotes sequences
T5219 20984-20990 SO_EXT:0000188 denotes intron
T5218 20975-20983 SO:0000357 denotes flanking
T5217 20952-20957 SO_EXT:0000147 denotes exons
T5216 20926-20933 SO_EXT:0001026 denotes genomic
T5215 20920-20925 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes Acadm
T5214 20918-20919 CHEBI_SO_EXT:base denotes b
T5213 20897-20904 SO_EXT:0000155 denotes plasmid
T5212 20820-20826 SO_EXT:0000440 denotes vector
T5211 20803-20815 SO_EXT:sequence_circularization denotes circularized
T5210 20774-20782 GO:0006266 denotes ligation
T5209 20755-20761 CHEBI_GO_SO_EXT:enzyme denotes enzyme
T5208 20681-20688 SO_EXT:0000155 denotes plasmid
T5636 23018-23024 UBERON:0000062 denotes organs
T5635 22937-22942 UBERON:0001153 denotes cecum
T5634 22921-22932 UBERON:0001728 denotes nasopharynx
T5633 22899-22908 NCBITaxon:2 denotes bacterial
T5632 22882-22889 NCBITaxon:10239 denotes viruses
T5631 22845-22856 CHMO_UBERON_EXT:bodily_fluid_or_bodily_fluid_experimental_method denotes serological
T5630 22820-22825 NCBITaxon:10088 denotes mouse
T5629 22797-22801 NCBITaxon:10088 denotes Mice
T5628 22791-22795 NCBITaxon:10088 denotes mice
T5207 20609-20622 SO_EXT:0005853 denotes gene cassette
T5206 20589-20597 CHEBI:7507 denotes neomycin
T5205 20569-20585 SO_EXT:0001644 denotes targeting vector
T5204 20564-20568 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T4884 19727-19731 GO_PR_EXT:long_chain_acyl_CoA_dehydrogenase denotes LCAD
T4883 19688-19692 NCBITaxon:10088 denotes mice
T4882 19686-19687 SO_EXT:sequence_nullness_or_absence denotes −
T4881 19684-19685 SO_EXT:sequence_nullness_or_absence denotes −
T4880 19680-19684 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T4879 19618-19640 GO_EXT:0003995 denotes acyl-CoA dehydrogenase
T4878 19618-19626 CHEBI:17984 denotes acyl-CoA
T4877 19601-19606 NCBITaxon:10088 denotes mouse
T4876 19535-19541 NCBITaxon:9606 denotes humans
T4875 19516-19520 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T4874 19419-19423 NCBITaxon:10088 denotes mice
T4873 19394-19401 UBERON:0000948 denotes cardiac
T4872 19384-19389 UBERON:0002107 denotes liver
T4871 19361-19365 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T4870 19355-19360 NCBITaxon:10088 denotes mouse
T4869 19291-19296 NCBITaxon:10088 denotes mouse
T4868 19267-19274 UBERON:0000948 denotes cardiac
T4867 19212-19214 PR_EXT:000005838 denotes -2
T4866 19202-19205 _FRAGMENT denotes CPT
T4865 19202-19207 PR_EXT:000005835 denotes CPT-1
T4864 19202-19205 GO_EXT:0004095 denotes CPT
T4863 19172-19175 CHEBI_UBERON_EXT:triglyceride_or_adipose_tissue denotes fat
T4862 19161-19186 GO:0001676 denotes long chain fat metabolism
T4861 19161-19175 CHEBI:15904 denotes long chain fat
T4860 19116-19121 GO_PR_EXT:very_long_chain_acyl_CoA_dehydrogenase denotes VLCAD
T4859 19031-19035 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T4858 18973-18980 UBERON:0000948 denotes cardiac
T4857 18949-18953 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T4856 18943-18948 NCBITaxon:9606 denotes human
T4855 18913-18917 NCBITaxon:10088 denotes mice
T4854 18911-18912 SO_EXT:sequence_nullness_or_absence denotes −
T4853 18909-18910 SO_EXT:sequence_nullness_or_absence denotes −
T4852 18905-18909 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T4851 18884-18892 GO_PATO_EXT:necrosis denotes necrosis
T4850 18859-18866 CL:0000187 denotes myocyte
T4849 18757-18761 NCBITaxon:10088 denotes mice
T4848 18755-18756 SO_EXT:sequence_nullness_or_absence denotes −
T4847 18753-18754 SO_EXT:sequence_nullness_or_absence denotes −
T4846 18749-18753 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T4845 18730-18737 UBERON:0000948 denotes cardiac
T4844 18686-18690 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T4843 18680-18685 NCBITaxon:9606 denotes human
T4842 18615-18619 NCBITaxon:10088 denotes mice
T4841 18613-18614 SO_EXT:sequence_nullness_or_absence denotes −
T4840 18611-18612 SO_EXT:sequence_nullness_or_absence denotes −
T4839 18607-18611 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T4838 18574-18581 UBERON:0002107 denotes hepatic
T4837 18564-18573 GO:0031982 denotes vesicular
T4836 18545-18554 GO:0031982 denotes vesicular
T4835 18530-18534 CHEBI_SO_EXT:mRNA denotes mRNA
T4834 18524-18529 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes Acadm
T4833 18499-18508 GO:0010467 denotes expresses
T4832 18472-18485 GO:0031649 denotes thermogenesis
T4831 18435-18438 CHEBI_UBERON_EXT:triglyceride_or_adipose_tissue denotes fat
T4830 18429-18438 UBERON:0001348 denotes Brown fat
T4829 18411-18415 UBERON_EXT:body denotes body
T4828 18382-18386 NCBITaxon:10088 denotes mice
T4827 18380-18381 SO_EXT:sequence_nullness_or_absence denotes −
T4826 18378-18379 SO_EXT:sequence_nullness_or_absence denotes −
T4825 18374-18378 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T4824 18279-18283 NCBITaxon:10088 denotes mice
T4823 18277-18278 SO_EXT:sequence_nullness_or_absence denotes −
T4822 18275-18276 SO_EXT:sequence_nullness_or_absence denotes −
T4821 18271-18275 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T4820 18265-18270 UBERON:0007023 denotes adult
T4819 18216-18224 GO:0007631 denotes ingested
T4818 18204-18208 UBERON:0001913 denotes milk
T4817 18102-18106 NCBITaxon_UBERON_EXT:pup denotes pups
T4816 18100-18101 SO_EXT:sequence_nullness_or_absence denotes −
T4815 18098-18099 SO_EXT:sequence_nullness_or_absence denotes −
T4814 18094-18098 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T4813 18085-18093 PATO_UBERON_EXT:neonate_or_newborn denotes neonatal
T4812 18057-18061 UBERON:0000104 denotes life
T4811 17901-17906 NCBITaxon:9606 denotes Human
T4810 17862-17867 GO:0016265 denotes dying
T4809 17857-17861 NCBITaxon_UBERON_EXT:pup denotes pups
T4808 17855-17856 SO_EXT:sequence_nullness_or_absence denotes −
T4807 17853-17854 SO_EXT:sequence_nullness_or_absence denotes −
T4806 17849-17853 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T4805 17800-17808 PATO_UBERON_EXT:neonate_or_newborn denotes neonatal
T4804 17773-17777 NCBITaxon:10088 denotes mice
T4803 17771-17772 SO_EXT:sequence_nullness_or_absence denotes −
T4802 17769-17770 SO_EXT:sequence_nullness_or_absence denotes −
T4801 17765-17769 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T4800 17740-17744 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T4799 17626-17630 NCBITaxon:10088 denotes mice
T4798 17624-17625 SO_EXT:sequence_nullness_or_absence denotes −
T4797 17622-17623 SO_EXT:sequence_nullness_or_absence denotes −
T4796 17618-17622 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T4795 17596-17604 PATO_UBERON_EXT:neonate_or_newborn denotes neonatal
T4794 17554-17577 GO_EXT:0003995 denotes acyl-CoA dehydrogenases
T4793 17554-17562 CHEBI:17984 denotes acyl-CoA
T4792 17418-17422 NCBITaxon:10088 denotes mice
T4791 17416-17417 SO_EXT:sequence_nullness_or_absence denotes −
T4790 17414-17415 SO_EXT:sequence_nullness_or_absence denotes −
T4789 17410-17414 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T4788 17390-17397 CHEBI_EXT:59132 denotes antigen
T4787 17385-17389 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T4786 17333-17346 CHEBI:15525 denotes palmitoyl-CoA
T4785 17305-17308 CHEBI:15346 denotes CoA
T4784 17259-17263 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T4783 17238-17243 NCBITaxon:9606 denotes human
T4782 17215-17218 CHEBI:15346 denotes CoA
T4781 17198-17211 MOP:0000590 denotes dehydrogenate
T4780 17154-17158 NCBITaxon:10088 denotes mice
T4779 17152-17153 SO_EXT:sequence_nullness_or_absence denotes −
T4778 17150-17151 SO_EXT:sequence_nullness_or_absence denotes −
T4777 17146-17150 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T4776 17129-17138 CHEBI_GO_SO_EXT:enzyme denotes enzymatic
T4775 17117-17121 NCBITaxon:10088 denotes mice
T4774 17115-17116 SO_EXT:sequence_nullness_or_absence denotes −
T4773 17113-17114 SO_EXT:sequence_nullness_or_absence denotes −
T4772 17109-17113 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T4771 17056-17061 UBERON:0002107 denotes liver
T4770 17036-17045 MOP:0000569 denotes reduction
T4769 17032-17035 GO:0045251 denotes ETF
T4768 17010-17014 CHEBI:22221 denotes acyl
T4767 16980-16989 CHEBI_GO_SO_EXT:enzyme denotes enzymatic
T4766 16960-16965 GO_PR_EXT:very_long_chain_acyl_CoA_dehydrogenase denotes VLCAD
T4765 16954-16959 NCBITaxon:9606 denotes human
T4764 16923-16927 CHEBI:22221 denotes acyl
T4763 16891-16896 GO_PR_EXT:very_long_chain_acyl_CoA_dehydrogenase denotes VLCAD
T4762 16885-16890 NCBITaxon:10088 denotes mouse
T4761 16872-16877 GO_PR_EXT:very_long_chain_acyl_CoA_dehydrogenase denotes VLCAD
T4760 16832-16870 GO_PR_EXT:very_long_chain_acyl_CoA_dehydrogenase denotes very long-chain acyl-CoA dehydrogenase
T4759 16832-16856 CHEBI:61910 denotes very long-chain acyl-CoA
T4758 16782-16788 CHEBI_GO_SO_EXT:enzyme denotes enzyme
T4757 16777-16781 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T4756 16771-16776 NCBITaxon:9606 denotes human
T4755 16711-16715 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T4754 16705-16710 NCBITaxon:10088 denotes mouse
T4753 16662-16675 CHEBI:17387 denotes Acylcarnitine
T4752 16656-16660 NCBITaxon:10088 denotes mice
T4751 16654-16655 SO_EXT:sequence_nullness_or_absence denotes −
T4750 16652-16653 SO_EXT:sequence_nullness_or_absence denotes −
T4749 16648-16652 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T4748 16611-16626 CHEBI:64390 denotes hexanoylglycine
T4747 16603-16610 UBERON:0001088 denotes urinary
T4746 16543-16547 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T4745 16523-16529 CHEBI_GO_SO_EXT:enzyme denotes enzyme
T4744 16471-16482 CHEBI_EXT:25212 denotes metabolites
T4743 16465-16470 UBERON:0001088 denotes urine
T4742 16454-16460 UBERON:0001969 denotes plasma
T4741 16416-16420 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T4740 16400-16405 NCBITaxon:9606 denotes human
T4739 16353-16364 CHEBI_EXT:biochemical denotes biochemical
T4738 16298-16302 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T4737 16282-16287 NCBITaxon:10088 denotes mouse
T4736 16265-16270 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes Acadm
T4897 19904-19909 UBERON:0002107 denotes liver
T4896 19887-19892 CHEBI_UBERON_EXT:triglyceride_or_adipose_tissue denotes fatty
T4895 19828-19835 SO_EXT:sequence_altered_entity denotes mutants
T4894 19790-19795 NCBITaxon:10088 denotes mouse
T4893 19788-19789 SO_EXT:sequence_nullness_or_absence denotes −
T4892 19786-19787 SO_EXT:sequence_nullness_or_absence denotes −
T4891 19782-19786 GO_PR_EXT:short_chain_acyl_CoA_dehydrogenase denotes SCAD
T4890 19777-19778 SO_EXT:sequence_nullness_or_absence denotes −
T4889 19775-19776 SO_EXT:sequence_nullness_or_absence denotes −
T4888 19770-19775 GO_PR_EXT:very_long_chain_acyl_CoA_dehydrogenase denotes VLCAD
T4887 19735-19739 NCBITaxon:10088 denotes mice
T4886 19733-19734 SO_EXT:sequence_nullness_or_absence denotes −
T4885 19731-19732 SO_EXT:sequence_nullness_or_absence denotes −
T3671 16214-16228 UBERON:0001013 denotes adipose tissue
T3670 16121-16128 CHEBI_EXT:26130 denotes pigment
T3669 16038-16044 UBERON:0000479 denotes tissue
T3650 14967-14971 NCBITaxon:10088 denotes mice
T3649 14965-14966 SO_EXT:sequence_nullness_or_absence denotes −
T3648 14963-14964 SO_EXT:sequence_nullness_or_absence denotes −
T3647 14959-14963 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T3646 14931-14938 UBERON:0000948 denotes cardiac
T3645 14881-14888 UBERON:0002107 denotes hepatic
T3644 14851-14855 NCBITaxon:10088 denotes mice
T3643 14849-14850 SO_EXT:sequence_nullness_or_absence denotes −
T3642 14847-14848 SO_EXT:sequence_nullness_or_absence denotes −
T3641 14843-14847 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T3640 14810-14816 UBERON:0000062 denotes organs
T3639 14742-14746 NCBITaxon:10088 denotes mice
T3638 14740-14741 SO_EXT:sequence_nullness_or_absence denotes −
T3637 14738-14739 SO_EXT:sequence_nullness_or_absence denotes −
T3636 14734-14738 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T3635 14728-14729 SO_EXT:normal_or_wild_type_or_present denotes +
T3634 14726-14727 SO_EXT:normal_or_wild_type_or_present denotes +
T3633 14722-14726 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T3632 14642-14646 NCBITaxon:10088 denotes mice
T3631 14640-14641 SO_EXT:normal_or_wild_type_or_present denotes +
T3630 14638-14639 SO_EXT:normal_or_wild_type_or_present denotes +
T3629 14634-14638 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T3628 14621-14625 NCBITaxon:10088 denotes mice
T3627 14619-14620 SO_EXT:sequence_nullness_or_absence denotes −
T3626 14617-14618 SO_EXT:sequence_nullness_or_absence denotes −
T3625 14613-14617 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T3624 14581-14588 UBERON:0002107 denotes hepatic
T3623 14571-14580 GO:0031982 denotes vesicular
T3622 14552-14561 GO:0031982 denotes vesicular
T3621 14503-14507 NCBITaxon:10088 denotes mice
T3620 14493-14494 SO_EXT:normal_or_wild_type_or_present denotes +
T3619 14491-14492 SO_EXT:normal_or_wild_type_or_present denotes +
T3618 14487-14491 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T3617 14476-14482 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T3291 13764-13777 CHEBI:17387 denotes acylcarnitine
T2864 12406-12412 UBERON:0001052 denotes rectal
T2863 12348-12352 NCBITaxon:10088 denotes mice
T3290 13745-13750 NCBITaxon:10088 denotes mouse
T3262 13205-13209 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T3261 13199-13200 SO_EXT:normal_or_wild_type_or_present denotes +
T3260 13197-13198 SO_EXT:normal_or_wild_type_or_present denotes +
T3259 13193-13197 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T3258 13160-13169 CHEBI:17126 denotes carnitine
T3257 13154-13159 UBERON:0001977 denotes serum
T3256 13104-13108 NCBITaxon:10088 denotes mice
T3255 13102-13103 SO_EXT:sequence_nullness_or_absence denotes −
T3254 13100-13101 SO_EXT:sequence_nullness_or_absence denotes −
T3253 13096-13100 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T3252 13090-13091 SO_EXT:normal_or_wild_type_or_present denotes +
T3251 13088-13089 SO_EXT:normal_or_wild_type_or_present denotes +
T3250 13084-13088 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T3249 13042-13051 SO_EXT:genotype_or_entity_with_genotype denotes genotypes
T3248 13037-13041 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T3247 12965-12976 CHEBI:15344 denotes acetoacetic
T3246 12944-12960 CHEBI:20067 denotes β-hydroxybutyric
T3245 12909-12913 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T3244 12878-12889 CHEBI:30832 denotes Adipic acid
T3243 12853-12866 CHEBI:64709 denotes organic acids
T3242 12785-12786 SO_EXT:normal_or_wild_type_or_present denotes +
T3241 12783-12784 SO_EXT:normal_or_wild_type_or_present denotes +
T3240 12779-12783 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T3239 12748-12763 CHEBI:64390 denotes hexanoylglycine
T3238 12730-12743 CHEBI:41865 denotes sebacic acids
T3237 12738-12743 CHEBI:9300 denotes acids
T3236 12717-12724 _FRAGMENT denotes suberic
T3235 12738-12743 CHEBI:30832 denotes acids
T3234 12709-12715 _FRAGMENT denotes adipic
T3233 12653-12657 NCBITaxon:10088 denotes mice
T3232 12651-12652 SO_EXT:sequence_nullness_or_absence denotes −
T3231 12649-12650 SO_EXT:sequence_nullness_or_absence denotes −
T3230 12645-12649 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T3229 12593-12598 NCBITaxon:9606 denotes human
T3228 12578-12582 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T3227 12546-12558 CHEBI:64709 denotes organic acid
T3226 12528-12532 NCBITaxon:10088 denotes mice
T3225 12526-12527 SO_EXT:sequence_nullness_or_absence denotes −
T3224 12524-12525 SO_EXT:sequence_nullness_or_absence denotes −
T3223 12520-12524 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T3222 12484-12496 CHEBI:64709 denotes organic acid
T3221 12478-12483 UBERON:0001088 denotes Urine
T3220 12455-12468 CHEBI:17387 denotes Acylcarnitine
T3219 12438-12450 CHEBI:64709 denotes Organic Acid
T2507 10977-10978 SO_EXT:sequence_nullness_or_absence denotes −
T2506 10973-10977 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T2505 10942-10946 GO:0016265 denotes loss
T2504 10933-10941 PATO_UBERON_EXT:neonate_or_newborn denotes neonatal
T2503 10900-10904 NCBITaxon:10088 denotes mice
T2502 10898-10899 SO_EXT:normal_or_wild_type_or_present denotes +
T2501 10896-10897 SO_EXT:normal_or_wild_type_or_present denotes +
T2500 10892-10896 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T2499 10845-10849 NCBITaxon:10088 denotes mice
T2498 10843-10844 SO_EXT:sequence_nullness_or_absence denotes −
T2497 10841-10842 SO_EXT:sequence_nullness_or_absence denotes −
T2496 10837-10841 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T2495 10819-10823 NCBITaxon:10088 denotes mice
T2494 10817-10818 SO_EXT:sequence_nullness_or_absence denotes −
T2493 10815-10816 SO_EXT:sequence_nullness_or_absence denotes −
T2492 10811-10815 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T2491 10805-10806 SO_EXT:normal_or_wild_type_or_present denotes +
T2490 10803-10804 SO_EXT:normal_or_wild_type_or_present denotes +
T2489 10799-10803 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T2488 10789-10793 GO:0007567 denotes born
T2487 10779-10783 NCBITaxon_UBERON_EXT:pup denotes pups
T2486 10747-10751 NCBITaxon_UBERON_EXT:pup denotes pups
T2485 10745-10746 SO_EXT:sequence_nullness_or_absence denotes −
T2484 10743-10744 SO_EXT:sequence_nullness_or_absence denotes −
T2483 10739-10743 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T2482 10707-10715 PATO_UBERON_EXT:neonate_or_newborn denotes neonatal
T2481 10688-10694 GO:0016265 denotes Deaths
T3289 13722-13728 NCBITaxon:9606 denotes humans
T3288 13705-13718 CHEBI:17387 denotes acylcarnitine
T3287 13662-13666 NCBITaxon:10088 denotes mice
T3286 13655-13661 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T3285 13628-13642 CHEBI:17387 denotes acylcarnitines
T3284 13563-13568 NCBITaxon:9606 denotes Human
T3283 13526-13530 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T3282 13520-13525 NCBITaxon:9606 denotes human
T3281 13487-13491 NCBITaxon:10088 denotes mice
T3280 13485-13486 SO_EXT:sequence_nullness_or_absence denotes −
T2862 12346-12347 SO_EXT:normal_or_wild_type_or_present denotes +
T2861 12344-12345 SO_EXT:normal_or_wild_type_or_present denotes +
T2860 12340-12344 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T2859 12295-12299 NCBITaxon:10088 denotes mice
T2858 12293-12294 SO_EXT:sequence_nullness_or_absence denotes −
T2857 12291-12292 SO_EXT:sequence_nullness_or_absence denotes −
T2856 12287-12291 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T2855 12233-12237 NCBITaxon:10088 denotes mice
T2854 12231-12232 SO_EXT:sequence_nullness_or_absence denotes −
T2853 12229-12230 SO_EXT:sequence_nullness_or_absence denotes −
T2852 12225-12229 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T2851 12129-12135 UBERON:0001052 denotes Rectal
T2850 12115-12119 NCBITaxon:10088 denotes mice
T2849 12113-12114 SO_EXT:normal_or_wild_type_or_present denotes +
T2848 12111-12112 SO_EXT:normal_or_wild_type_or_present denotes +
T2847 12107-12111 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T2846 12055-12059 NCBITaxon:10088 denotes mice
T2845 12053-12054 SO_EXT:sequence_nullness_or_absence denotes −
T2844 12051-12052 SO_EXT:sequence_nullness_or_absence denotes −
T2843 12047-12051 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T2842 12025-12031 UBERON:0001052 denotes rectal
T2841 11968-11978 GO_EXT:fatality_or_lethality denotes fatalities
T2840 11859-11863 NCBITaxon:10088 denotes mice
T2839 11857-11858 SO_EXT:sequence_nullness_or_absence denotes −
T2838 11855-11856 SO_EXT:sequence_nullness_or_absence denotes −
T2837 11851-11855 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T2836 11772-11776 NCBITaxon:10088 denotes mice
T2835 11715-11719 NCBITaxon:10088 denotes mice
T2834 11713-11714 SO_EXT:normal_or_wild_type_or_present denotes +
T2833 11711-11712 SO_EXT:normal_or_wild_type_or_present denotes +
T2832 11707-11711 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T2831 11633-11643 CHEBI:35366 denotes fatty acid
T2830 11633-11638 CHEBI_UBERON_EXT:triglyceride_or_adipose_tissue denotes fatty
T2829 11622-11627 UBERON:0001977 denotes serum
T2828 11601-11608 CHEBI:17234 denotes glucose
T2827 11595-11600 UBERON:0001977 denotes serum
T2826 11574-11578 NCBITaxon:10088 denotes mice
T2825 11572-11573 SO_EXT:sequence_nullness_or_absence denotes −
T2824 11570-11571 SO_EXT:sequence_nullness_or_absence denotes −
T2823 11566-11570 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T2822 11515-11519 NCBITaxon:10088 denotes mice
T2821 11500-11504 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T2542 11408-11415 GO:0007618 denotes matings
T2541 11334-11341 GO:0007618 denotes matings
T2540 11332-11333 SO_EXT:sequence_nullness_or_absence denotes −
T2539 11330-11331 SO_EXT:sequence_nullness_or_absence denotes −
T2538 11326-11330 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T2537 11322-11323 SO_EXT:sequence_nullness_or_absence denotes −
T2536 11320-11321 SO_EXT:sequence_nullness_or_absence denotes −
T2535 11316-11320 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T2534 11303-11310 GO:0007618 denotes matings
T2533 11301-11302 SO_EXT:normal_or_wild_type_or_present denotes +
T2532 11299-11300 SO_EXT:normal_or_wild_type_or_present denotes +
T2531 11295-11299 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T2530 11291-11292 SO_EXT:normal_or_wild_type_or_present denotes +
T2529 11289-11290 SO_EXT:normal_or_wild_type_or_present denotes +
T2528 11285-11289 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T2527 11253-11261 SO_EXT:sequence_alteration_entity_or_process denotes mutation
T2526 11243-11252 SO_EXT:sequence_insertion_entity_or_process denotes insertion
T2525 11213-11222 CHEBI_EXT:polyatomic_entity_or_group denotes molecular
T2524 11205-11209 NCBITaxon_UBERON_EXT:pup denotes pups
T2523 11190-11191 SO_EXT:sequence_nullness_or_absence denotes −
T2522 11188-11189 SO_EXT:normal_or_wild_type_or_present denotes +
T2521 11184-11188 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T2520 11167-11174 SO_EXT:sequence_altered_entity denotes mutants
T2519 11165-11166 SO_EXT:sequence_nullness_or_absence denotes −
T2518 11163-11164 SO_EXT:sequence_nullness_or_absence denotes −
T2517 11159-11163 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T2516 11108-11109 SO_EXT:normal_or_wild_type_or_present denotes +
T2515 11106-11107 SO_EXT:normal_or_wild_type_or_present denotes +
T2514 11102-11106 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T2513 11029-11033 NCBITaxon_UBERON_EXT:pup denotes pups
T2512 11027-11028 SO_EXT:normal_or_wild_type_or_present denotes +
T2511 11025-11026 SO_EXT:normal_or_wild_type_or_present denotes +
T2510 11021-11025 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T2509 10981-10985 NCBITaxon_UBERON_EXT:pup denotes pups
T2508 10979-10980 SO_EXT:sequence_nullness_or_absence denotes −
T894 4088-4093 NCBITaxon:10088 denotes mouse
T6661 26263-26264 SO_EXT:sequence_nullness_or_absence denotes −
T6657 26239-26243 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T6656 26202-26207 UBERON:0002107 denotes liver
T6655 26196-26200 NCBITaxon:10088 denotes mice
T6654 26179-26183 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T6653 26141-26147 CHEBI_GO_SO_EXT:enzyme denotes enzyme
T6652 26135-26140 UBERON:0002107 denotes Liver
T2480 10679-10687 PATO_UBERON_EXT:neonate_or_newborn denotes Neonatal
T2217 9084-9085 SO_EXT:normal_or_wild_type_or_present denotes +
T2218 9086-9087 SO_EXT:normal_or_wild_type_or_present denotes +
T2219 9088-9092 NCBITaxon:10088 denotes mice
T2220 9105-9109 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T2221 9109-9110 SO_EXT:sequence_nullness_or_absence denotes −
T2222 9111-9112 SO_EXT:sequence_nullness_or_absence denotes −
T2223 9113-9117 NCBITaxon:10088 denotes mice
T2224 9158-9178 CHEBI:61905 denotes short-chain acyl-CoA
T2225 9158-9192 GO_PR_EXT:short_chain_acyl_CoA_dehydrogenase denotes short-chain acyl-CoA dehydrogenase
T2226 9194-9198 GO_PR_EXT:short_chain_acyl_CoA_dehydrogenase denotes SCAD
T2227 9246-9256 GO:0010467 denotes expression
T2228 9260-9264 GO_PR_EXT:short_chain_acyl_CoA_dehydrogenase denotes SCAD
T2229 9265-9272 CHEBI_PR_EXT:protein denotes protein
T2230 9281-9285 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T2231 9285-9286 SO_EXT:normal_or_wild_type_or_present denotes +
T2232 9287-9288 SO_EXT:normal_or_wild_type_or_present denotes +
T2233 9293-9297 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T2234 9297-9298 SO_EXT:sequence_nullness_or_absence denotes −
T2235 9299-9300 SO_EXT:sequence_nullness_or_absence denotes −
T2236 9301-9305 NCBITaxon:10088 denotes mice
T2237 9858-9864 CHEBI_GO_SO_EXT:enzyme denotes Enzyme
T2238 9890-9895 NCBITaxon:10088 denotes mouse
T2239 9896-9901 UBERON:0002107 denotes liver
T2240 9924-9932 CHEBI:10545 denotes electron
T2241 9924-9942 MOP:0000615 denotes electron transport
T2242 9924-9955 GO:0045251 denotes electron transport flavoprotein
T2243 9933-9942 GO_EXT:biological_routing_or_transport denotes transport
T2244 9943-9955 CHEBI:5086 denotes flavoprotein
T2245 9957-9960 GO:0045251 denotes ETF
T2246 9962-9971 MOP:0000569 denotes reduction
T2247 9983-9995 CHEBI:15533 denotes octanoyl-CoA
T2248 10007-10020 CHEBI:15525 denotes palmitoyl-CoA
T2249 10044-10048 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T2250 10048-10049 SO_EXT:sequence_nullness_or_absence denotes −
T2251 10050-10051 SO_EXT:sequence_nullness_or_absence denotes −
T2252 10052-10056 NCBITaxon:10088 denotes mice
T2253 10099-10112 MOP:0000590 denotes dehydrogenate
T5242 21562-21570 GO:0006266 denotes ligation
T3268 13229-13230 SO_EXT:sequence_nullness_or_absence denotes −
T3267 13227-13228 SO_EXT:sequence_nullness_or_absence denotes −
T3266 13223-13227 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T3265 13213-13217 NCBITaxon:10088 denotes mice
T3264 13211-13212 SO_EXT:sequence_nullness_or_absence denotes −
T3263 13209-13210 SO_EXT:sequence_nullness_or_absence denotes −
T3279 13483-13484 SO_EXT:sequence_nullness_or_absence denotes −
T3278 13479-13483 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T3277 13449-13462 CHEBI:17387 denotes acylcarnitine
T3276 13417-13422 UBERON:0001977 denotes serum
T3275 13389-13402 CHEBI:17387 denotes acylcarnitine
T3274 13347-13360 CHEBI:17387 denotes acylcarnitine
T3273 13342-13346 UBERON:0001970 denotes Bile
T3272 13307-13320 CHEBI:17387 denotes acylcarnitine
T3271 13274-13291 CHEBI:86063 denotes decenoylcarnitine
T3270 13268-13273 UBERON:0001977 denotes serum
T3269 13231-13235 NCBITaxon:10088 denotes mice
T3668 15983-15988 UBERON:0000948 denotes heart
T3667 15958-15963 UBERON:0004663 denotes aorta
T3666 15946-15953 _FRAGMENT denotes wall of
T3665 15905-15913 CL:0000187 denotes myocytes
T3664 15840-15848 GO:0005730 denotes nucleoli
T3663 15812-15821 GO:0031982 denotes vesicular
T3662 15781-15789 CL:0000187 denotes myocytes
T3661 15762-15768 GO:0005634 denotes Nuclei
T3660 15739-15751 CL:0000771 denotes eosinophilic
T3659 15707-15717 GO:0030016 denotes myofibrils
T3658 15680-15690 GO:0016528 denotes sarcoplasm
T3657 15613-15621 CL:0000187 denotes myocytes
T3656 15560-15568 GO_PATO_EXT:necrosis denotes necrosis
T3655 15535-15542 CL:0000187 denotes myocyte
T3654 15471-15476 NCBITaxon:10088 denotes mouse
T3653 15469-15470 SO_EXT:sequence_nullness_or_absence denotes −
T3652 15467-15468 SO_EXT:sequence_nullness_or_absence denotes −
T3651 15463-15467 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T890 3847-3853 NCBITaxon:33208 denotes animal
T891 3864-3868 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T892 3934-3938 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T893 3996-4001 NCBITaxon:9606 denotes human
T895 4103-4107 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T896 4122-4126 SO_EXT:0000704 denotes gene
T897 4140-4149 UBERON:0000922 denotes embryonic
T898 4140-4154 _FRAGMENT denotes embryonic stem
T899 4160-4165 CL:0002322 denotes cells
T900 4156-4158 _FRAGMENT denotes ES
T901 4160-4165 CL_GO_EXT:cell denotes cells
T902 4193-4199 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T903 4200-4204 NCBITaxon:10088 denotes mice
T904 4271-4276 NCBITaxon:9606 denotes human
T905 4277-4281 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T1478 4349-4353 SO_EXT:0000704 denotes Gene
T1479 4382-4386 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T1480 4397-4401 NCBITaxon:10088 denotes Mice
T1481 4402-4406 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T1483 4417-4423 SO_EXT:0000440 denotes vector
T1520 5967-5974 CL:0002322 denotes ES cell
T1521 5970-5974 CL_GO_EXT:cell denotes cell
T1522 5975-5981 SO_EXT:sequence_cloned_entity denotes clones
T1523 6092-6096 SO_EXT:0000147 denotes exon
T1524 6100-6105 CHEBI_SO_EXT:molecular_probe denotes probe
T1525 6107-6112 CHEBI_SO_EXT:molecular_probe denotes probe
T1526 6136-6152 SO_EXT:0001644 denotes targeting vector
T1527 6154-6164 GO:0097617 denotes hybridized
T1528 6176-6177 CHEBI_SO_EXT:base denotes b
T1529 6207-6208 CHEBI_SO_EXT:base denotes b
T1530 6245-6254 SO_EXT:sequence_insertion_entity_or_process denotes insertion
T1531 6262-6268 SO_EXT:0000440 denotes vector
T1532 6276-6281 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes Acadm
T1533 6320-6327 CL:0002322 denotes ES cell
T4925 20484-20495 CHEBI:35366 denotes fatty acids
T4924 20484-20489 CHEBI_UBERON_EXT:triglyceride_or_adipose_tissue denotes fatty
T4923 20397-20406 MOP:0000568 denotes oxidation
T4922 20381-20394 GO:0005739 denotes mitochondrial
T4921 20330-20335 NCBITaxon:10088 denotes mouse
T4920 20315-20319 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T4919 20272-20279 NCBITaxon:33208 denotes animals
T4918 20240-20244 NCBITaxon:10088 denotes mice
T4917 20238-20239 SO_EXT:sequence_nullness_or_absence denotes −
T4916 20236-20237 SO_EXT:sequence_nullness_or_absence denotes −
T4915 20232-20236 GO_PR_EXT:short_chain_acyl_CoA_dehydrogenase denotes SCAD
T4914 20226-20227 SO_EXT:sequence_nullness_or_absence denotes −
T4913 20224-20225 SO_EXT:sequence_nullness_or_absence denotes −
T4912 20219-20224 GO_PR_EXT:very_long_chain_acyl_CoA_dehydrogenase denotes VLCAD
T4911 20112-20117 NCBITaxon:10088 denotes mouse
T4910 20075-20079 NCBITaxon:10088 denotes mice
T4909 20073-20074 SO_EXT:sequence_nullness_or_absence denotes −
T4908 20071-20072 SO_EXT:sequence_nullness_or_absence denotes −
T4907 20067-20071 GO_PR_EXT:medium_chain_acyl_CoA_dehydrogenase denotes MCAD
T4906 20045-20053 PATO_UBERON_EXT:neonate_or_newborn denotes neonatal
T4905 19976-19987 GO:0007565 denotes gestational
T4904 19965-19971 GO:0016265 denotes deaths
T4903 19938-19942 NCBITaxon:10088 denotes mice
T4902 19936-19937 SO_EXT:sequence_nullness_or_absence denotes −
T4901 19934-19935 SO_EXT:sequence_nullness_or_absence denotes −
T4900 19930-19934 GO_PR_EXT:long_chain_acyl_CoA_dehydrogenase denotes LCAD
T4899 19922-19928 UBERON:0002113 denotes kidney
T4898 19911-19916 UBERON:0000948 denotes heart
R120 T283 T282 _lexicallyChainedTo cells,embryonic stem
R121 T283 T284 _lexicallyChainedTo cells,ES
R576 T862 T861 _lexicallyChainedTo fatty acid,medium-chain length
R577 T899 T898 _lexicallyChainedTo cells,embryonic stem
R578 T899 T900 _lexicallyChainedTo cells,ES
R1234 T1915 T1914 _lexicallyChainedTo transcript,spliced
R2068 T3235 T3234 _lexicallyChainedTo acids,adipic
R2069 T3237 T3236 _lexicallyChainedTo acids,suberic
R2321 T3667 T3666 _lexicallyChainedTo aorta,wall of
R3111 T4867 T4866 _lexicallyChainedTo -2,CPT