PMC:1189073 / 37806-37906
Annnotations
craft-ca-core-ex-dev
{"project":"craft-ca-core-ex-dev","denotations":[{"id":"T18776","span":{"begin":26,"end":34},"obj":"CHEBI_PR_EXT:protein"},{"id":"T18777","span":{"begin":55,"end":61},"obj":"CHEBI_CHMO_EXT:buffer_solution"},{"id":"T18778","span":{"begin":93,"end":99},"obj":"CHEBI_CHMO_EXT:buffer_solution"}],"text":" Finally the precipitated proteins were washed in RIPA buffer and boiled in 50 μl of Laemmli buffer."}
craft-sa-dev
{"project":"craft-sa-dev","denotations":[{"id":"T19128","span":{"begin":0,"end":100},"obj":"sentence"},{"id":"T19129","span":{"begin":1,"end":8},"obj":"RB"},{"id":"T19130","span":{"begin":40,"end":46},"obj":"VBN"},{"id":"T19131","span":{"begin":9,"end":12},"obj":"DT"},{"id":"T19132","span":{"begin":26,"end":34},"obj":"NN"},{"id":"T19133","span":{"begin":13,"end":25},"obj":"VBN"},{"id":"T19134","span":{"begin":35,"end":39},"obj":"VBD"},{"id":"T19135","span":{"begin":47,"end":49},"obj":"IN"},{"id":"T19136","span":{"begin":50,"end":54},"obj":"NN"},{"id":"T19137","span":{"begin":55,"end":61},"obj":"NN"},{"id":"T19138","span":{"begin":62,"end":65},"obj":"CC"},{"id":"T19139","span":{"begin":66,"end":72},"obj":"VBN"},{"id":"T19140","span":{"begin":73,"end":75},"obj":"IN"},{"id":"T19141","span":{"begin":76,"end":78},"obj":"CD"},{"id":"T19142","span":{"begin":79,"end":81},"obj":"NN"},{"id":"T19143","span":{"begin":82,"end":84},"obj":"IN"},{"id":"T19144","span":{"begin":85,"end":92},"obj":"NNP"},{"id":"T19145","span":{"begin":93,"end":99},"obj":"NN"},{"id":"T19146","span":{"begin":99,"end":100},"obj":"."}],"relations":[{"id":"R5610","pred":"advmod","subj":"T19129","obj":"T19130"},{"id":"R5611","pred":"det","subj":"T19131","obj":"T19132"},{"id":"R5612","pred":"nsubjpass","subj":"T19132","obj":"T19130"},{"id":"R5613","pred":"amod","subj":"T19133","obj":"T19132"},{"id":"R5614","pred":"auxpass","subj":"T19134","obj":"T19130"},{"id":"R5615","pred":"prep","subj":"T19135","obj":"T19130"},{"id":"R5616","pred":"compound","subj":"T19136","obj":"T19137"},{"id":"R5617","pred":"pobj","subj":"T19137","obj":"T19135"},{"id":"R5618","pred":"cc","subj":"T19138","obj":"T19130"},{"id":"R5619","pred":"conj","subj":"T19139","obj":"T19130"},{"id":"R5620","pred":"prep","subj":"T19140","obj":"T19139"},{"id":"R5621","pred":"nummod","subj":"T19141","obj":"T19142"},{"id":"R5622","pred":"pobj","subj":"T19142","obj":"T19140"},{"id":"R5623","pred":"prep","subj":"T19143","obj":"T19142"},{"id":"R5624","pred":"compound","subj":"T19144","obj":"T19145"},{"id":"R5625","pred":"pobj","subj":"T19145","obj":"T19143"},{"id":"R5626","pred":"punct","subj":"T19146","obj":"T19130"}],"text":" Finally the precipitated proteins were washed in RIPA buffer and boiled in 50 μl of Laemmli buffer."}