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PMC:1189073 / 37119-37999 JSONTXT

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craft-ca-core-ex-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T18754 0-4 CL_GO_EXT:cell denotes Cell
T18755 0-12 GO:0009986 denotes Cell surface
T18756 13-26 MOP:0000093 denotes biotinylation
T18757 77-81 PR_EXT:000004182 denotes AQP2
T18758 93-104 GO:0009294 denotes transfected
T18759 115-120 CL_GO_EXT:cell denotes cells
T18760 128-138 GO:0010467 denotes expressing
T18761 139-148 SO_EXT:wild_type_entity_or_quality denotes wild-type
T18762 149-153 PR_EXT:000004182 denotes AQP2
T18763 158-163 CL_GO_EXT:cell denotes cells
T18764 181-187 SO_EXT:0000440 denotes vector
T18765 218-230 GO:0009294 denotes transfection
T18766 232-237 CL_GO_EXT:cell denotes cells
T18767 259-268 CHEBI:42471 denotes forskolin
T18768 270-281 GO_EXT:trypsinization denotes trypsinized
T18769 321-326 CL_GO_EXT:cell denotes cells
T18770 370-376 CHEBI:15956 denotes biotin
T18771 432-437 CL_GO_EXT:cell denotes Cells
T18772 464-468 CHEBI:9754 denotes Tris
T18773 512-521 GO:0016020 denotes membranes
T18774 584-593 GO:0016020 denotes membranes
T18775 625-634 GO_EXT:biological_movement_or_translocation_process denotes mobilized
T18776 713-721 CHEBI_PR_EXT:protein denotes proteins
T18777 742-748 CHEBI_CHMO_EXT:buffer_solution denotes buffer
T18778 780-786 CHEBI_CHMO_EXT:buffer_solution denotes buffer
T18779 794-799 CL_GO_EXT:cell denotes cells
T18780 808-820 MOP:0000093 denotes biotinylated
T18781 863-871 GO:0042571 denotes antibody
T18782 875-879 PR_EXT:000004182 denotes AQP2

craft-ca-core-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T18708 0-12 GO:0009986 denotes Cell surface
T18709 13-26 MOP:0000093 denotes biotinylation
T18710 77-81 PR:000004182 denotes AQP2
T18711 93-104 GO:0009294 denotes transfected
T18712 128-138 GO:0010467 denotes expressing
T18713 149-153 PR:000004182 denotes AQP2
T18714 181-187 SO:0000440 denotes vector
T18715 218-230 GO:0009294 denotes transfection
T18716 259-268 CHEBI:42471 denotes forskolin
T18717 370-376 CHEBI:15956 denotes biotin
T18718 464-468 CHEBI:9754 denotes Tris
T18719 512-521 GO:0016020 denotes membranes
T18720 584-593 GO:0016020 denotes membranes
T18721 808-820 MOP:0000093 denotes biotinylated
T18722 863-871 GO:0042571 denotes antibody
T18723 875-879 PR:000004182 denotes AQP2

craft-sa-dev

Id Subject Object Predicate Lexical cue
T18986 0-4 NN denotes Cell
T18987 13-26 NN denotes biotinylation
T18988 5-12 NN denotes surface
T18989 31-40 VBN denotes performed
T18990 27-30 VBD denotes was
T18991 41-43 IN denotes in
T18992 44-45 DT denotes a
T18993 54-60 NN denotes manner
T18994 46-53 JJ denotes similar
T18995 60-61 . denotes .
T18996 61-194 sentence denotes However, pEGFP-AQP2-F204V, was transfected into MDCK cells stably expressing wild-type AQP2 and cells made stable with vector alone.
T18997 62-69 RB denotes However
T18998 93-104 VBN denotes transfected
T18999 69-71 , denotes ,
T19000 71-76 NN denotes pEGFP
T19001 82-87 NN denotes F204V
T19002 76-77 HYPH denotes -
T19003 77-81 NN denotes AQP2
T19004 81-82 HYPH denotes -
T19005 87-89 , denotes ,
T19006 89-92 VBD denotes was
T19007 105-109 IN denotes into
T19008 110-114 NN denotes MDCK
T19009 115-120 NNS denotes cells
T19010 121-127 RB denotes stably
T19011 128-138 VBG denotes expressing
T19012 139-143 JJ denotes wild
T19013 144-148 NN denotes type
T19014 143-144 HYPH denotes -
T19015 149-153 NN denotes AQP2
T19016 154-157 CC denotes and
T19017 158-163 NNS denotes cells
T19018 164-168 VBN denotes made
T19019 169-175 JJ denotes stable
T19020 176-180 IN denotes with
T19021 181-187 NN denotes vector
T19022 188-193 RB denotes alone
T19023 193-194 . denotes .
T19024 194-431 sentence denotes Twenty-four hours post-transfection, cells were stimulated with forskolin, trypsinized, resuspended in 1 ml of PBS (2.5 × 106 cells/ml), and incubated with 0.5 mg of NHS-PEO4-biotin (Pierce Biotechnology) for 30 min at room temperature.
T19025 195-201 CD denotes Twenty
T19026 202-206 CD denotes four
T19027 201-202 HYPH denotes -
T19028 207-212 NNS denotes hours
T19029 213-230 RB denotes post-transfection
T19030 243-253 VBN denotes stimulated
T19031 230-232 , denotes ,
T19032 232-237 NNS denotes cells
T19033 238-242 VBD denotes were
T19034 254-258 IN denotes with
T19035 259-268 NN denotes forskolin
T19036 268-270 , denotes ,
T19037 270-281 VBN denotes trypsinized
T19038 281-283 , denotes ,
T19039 283-294 VBN denotes resuspended
T19040 295-297 IN denotes in
T19041 298-299 CD denotes 1
T19042 300-302 NN denotes ml
T19043 303-305 IN denotes of
T19044 306-309 NN denotes PBS
T19045 310-311 -LRB- denotes (
T19046 321-326 NNS denotes cells
T19047 311-314 CD denotes 2.5
T19048 317-320 CD denotes 106
T19049 315-316 SYM denotes ×
T19050 326-327 SYM denotes /
T19051 327-329 NNS denotes ml
T19052 329-330 -RRB- denotes )
T19053 330-332 , denotes ,
T19054 332-335 CC denotes and
T19055 336-345 VBN denotes incubated
T19056 346-350 IN denotes with
T19057 351-354 CD denotes 0.5
T19058 355-357 NN denotes mg
T19059 358-360 IN denotes of
T19060 361-364 NN denotes NHS
T19061 370-376 NN denotes biotin
T19062 364-365 HYPH denotes -
T19063 365-369 NN denotes PEO4
T19064 369-370 HYPH denotes -
T19065 377-378 -LRB- denotes (
T19066 385-398 NNP denotes Biotechnology
T19067 378-384 NNP denotes Pierce
T19068 398-399 -RRB- denotes )
T19069 400-403 IN denotes for
T19070 404-406 CD denotes 30
T19071 407-410 NN denotes min
T19072 411-413 IN denotes at
T19073 414-418 NN denotes room
T19074 419-430 NN denotes temperature
T19075 430-431 . denotes .
T19076 431-571 sentence denotes Cells were washed once in 10 mM Tris (pH 8) and three times in PBS, after which membranes were purified and solubilized as described above.
T19077 432-437 NNS denotes Cells
T19078 443-449 VBN denotes washed
T19079 438-442 VBD denotes were
T19080 450-454 RB denotes once
T19081 455-457 IN denotes in
T19082 458-460 CD denotes 10
T19083 461-463 NN denotes mM
T19084 464-468 NN denotes Tris
T19085 469-470 -LRB- denotes (
T19086 470-472 NN denotes pH
T19087 473-474 CD denotes 8
T19088 474-475 -RRB- denotes )
T19089 476-479 CC denotes and
T19090 480-485 CD denotes three
T19091 486-491 NNS denotes times
T19092 492-494 IN denotes in
T19093 495-498 NN denotes PBS
T19094 498-500 , denotes ,
T19095 500-505 IN denotes after
T19096 527-535 VBN denotes purified
T19097 506-511 WDT denotes which
T19098 512-521 NNS denotes membranes
T19099 522-526 VBD denotes were
T19100 536-539 CC denotes and
T19101 540-551 VBN denotes solubilized
T19102 552-554 IN denotes as
T19103 555-564 VBN denotes described
T19104 565-570 RB denotes above
T19105 570-571 . denotes .
T19106 571-687 sentence denotes Solubilized membranes were incubated with 20 μl of immobilized streptavidin (Pierce Biotechnology) for 2 h at 4 °C.
T19107 572-583 VBN denotes Solubilized
T19108 584-593 NNS denotes membranes
T19109 599-608 VBN denotes incubated
T19110 594-598 VBD denotes were
T19111 609-613 IN denotes with
T19112 614-616 CD denotes 20
T19113 617-619 NN denotes μl
T19114 620-622 IN denotes of
T19115 623-634 VBN denotes immobilized
T19116 635-647 NN denotes streptavidin
T19117 648-649 -LRB- denotes (
T19118 656-669 NNP denotes Biotechnology
T19119 649-655 NNP denotes Pierce
T19120 669-670 -RRB- denotes )
T19121 671-674 IN denotes for
T19122 675-676 CD denotes 2
T19123 677-678 NN denotes h
T19124 679-681 IN denotes at
T19125 682-683 CD denotes 4
T19126 684-686 NN denotes °C
T19127 686-687 . denotes .
T19128 687-787 sentence denotes Finally the precipitated proteins were washed in RIPA buffer and boiled in 50 μl of Laemmli buffer.
T19129 688-695 RB denotes Finally
T19130 727-733 VBN denotes washed
T19131 696-699 DT denotes the
T19132 713-721 NN denotes proteins
T19133 700-712 VBN denotes precipitated
T19134 722-726 VBD denotes were
T19135 734-736 IN denotes in
T19136 737-741 NN denotes RIPA
T19137 742-748 NN denotes buffer
T19138 749-752 CC denotes and
T19139 753-759 VBN denotes boiled
T19140 760-762 IN denotes in
T19141 763-765 CD denotes 50
T19142 766-768 NN denotes μl
T19143 769-771 IN denotes of
T19144 772-779 NNP denotes Laemmli
T19145 780-786 NN denotes buffer
T19146 786-787 . denotes .
T19147 787-880 sentence denotes Total cells and the biotinylated precipitates were immunoblotting using an antibody to AQP2.
T19148 788-793 JJ denotes Total
T19149 794-799 NNS denotes cells
T19150 839-853 VBG denotes immunoblotting
T19151 800-803 CC denotes and
T19152 804-807 DT denotes the
T19153 821-833 NNS denotes precipitates
T19154 808-820 VBN denotes biotinylated
T19155 834-838 VBD denotes were
T19156 854-859 VBG denotes using
T19157 860-862 DT denotes an
T19158 863-871 NN denotes antibody
T19159 872-874 IN denotes to
T19160 875-879 NN denotes AQP2
T19161 879-880 . denotes .
R5477 T18986 T18987 compound Cell,biotinylation
R5478 T18987 T18989 nsubjpass biotinylation,performed
R5479 T18988 T18987 compound surface,biotinylation
R5480 T18990 T18989 auxpass was,performed
R5481 T18991 T18989 prep in,performed
R5482 T18992 T18993 det a,manner
R5483 T18993 T18991 pobj manner,in
R5484 T18994 T18993 amod similar,manner
R5485 T18995 T18989 punct .,performed
R5486 T18997 T18998 advmod However,transfected
R5487 T18999 T18998 punct ", ",transfected
R5488 T19000 T19001 compound pEGFP,F204V
R5489 T19001 T18998 nsubjpass F204V,transfected
R5490 T19002 T19001 punct -,F204V
R5491 T19003 T19001 compound AQP2,F204V
R5492 T19004 T19001 punct -,F204V
R5493 T19005 T18998 punct ", ",transfected
R5494 T19006 T18998 auxpass was,transfected
R5495 T19007 T18998 prep into,transfected
R5496 T19008 T19009 compound MDCK,cells
R5497 T19009 T19007 pobj cells,into
R5498 T19010 T19011 advmod stably,expressing
R5499 T19011 T19009 acl expressing,cells
R5500 T19012 T19013 amod wild,type
R5501 T19013 T19015 compound type,AQP2
R5502 T19014 T19013 punct -,type
R5503 T19015 T19011 dobj AQP2,expressing
R5504 T19016 T19009 cc and,cells
R5505 T19017 T19009 conj cells,cells
R5506 T19018 T19017 acl made,cells
R5507 T19019 T19018 oprd stable,made
R5508 T19020 T19018 prep with,made
R5509 T19021 T19020 pobj vector,with
R5510 T19022 T19021 advmod alone,vector
R5511 T19023 T18998 punct .,transfected
R5512 T19025 T19026 compound Twenty,four
R5513 T19026 T19028 nummod four,hours
R5514 T19027 T19026 punct -,four
R5515 T19028 T19029 npadvmod hours,post-transfection
R5516 T19029 T19030 advmod post-transfection,stimulated
R5517 T19031 T19030 punct ", ",stimulated
R5518 T19032 T19030 nsubjpass cells,stimulated
R5519 T19033 T19030 auxpass were,stimulated
R5520 T19034 T19030 prep with,stimulated
R5521 T19035 T19034 pobj forskolin,with
R5522 T19036 T19030 punct ", ",stimulated
R5523 T19037 T19030 conj trypsinized,stimulated
R5524 T19038 T19037 punct ", ",trypsinized
R5525 T19039 T19037 conj resuspended,trypsinized
R5526 T19040 T19039 prep in,resuspended
R5527 T19041 T19042 nummod 1,ml
R5528 T19042 T19040 pobj ml,in
R5529 T19043 T19042 prep of,ml
R5530 T19044 T19043 pobj PBS,of
R5531 T19045 T19046 punct (,cells
R5532 T19046 T19042 parataxis cells,ml
R5533 T19047 T19048 quantmod 2.5,106
R5534 T19048 T19046 nummod 106,cells
R5535 T19049 T19048 punct ×,106
R5536 T19050 T19051 punct /,ml
R5537 T19051 T19046 prep ml,cells
R5538 T19052 T19046 punct ),cells
R5539 T19053 T19039 punct ", ",resuspended
R5540 T19054 T19039 cc and,resuspended
R5541 T19055 T19039 conj incubated,resuspended
R5542 T19056 T19055 prep with,incubated
R5543 T19057 T19058 nummod 0.5,mg
R5544 T19058 T19056 pobj mg,with
R5545 T19059 T19058 prep of,mg
R5546 T19060 T19061 compound NHS,biotin
R5547 T19061 T19059 pobj biotin,of
R5548 T19062 T19061 punct -,biotin
R5549 T19063 T19061 compound PEO4,biotin
R5550 T19064 T19061 punct -,biotin
R5551 T19065 T19066 punct (,Biotechnology
R5552 T19066 T19061 parataxis Biotechnology,biotin
R5553 T19067 T19066 compound Pierce,Biotechnology
R5554 T19068 T19066 punct ),Biotechnology
R5555 T19069 T19055 prep for,incubated
R5556 T19070 T19071 nummod 30,min
R5557 T19071 T19069 pobj min,for
R5558 T19072 T19055 prep at,incubated
R5559 T19073 T19074 compound room,temperature
R5560 T19074 T19072 pobj temperature,at
R5561 T19075 T19030 punct .,stimulated
R5562 T19077 T19078 nsubj Cells,washed
R5563 T19079 T19078 aux were,washed
R5564 T19080 T19078 advmod once,washed
R5565 T19081 T19078 prep in,washed
R5566 T19082 T19083 nummod 10,mM
R5567 T19083 T19084 compound mM,Tris
R5568 T19084 T19081 pobj Tris,in
R5569 T19085 T19086 punct (,pH
R5570 T19086 T19084 parataxis pH,Tris
R5571 T19087 T19086 nummod 8,pH
R5572 T19088 T19086 punct ),pH
R5573 T19089 T19078 cc and,washed
R5574 T19090 T19091 nummod three,times
R5575 T19091 T19092 npadvmod times,in
R5576 T19092 T19078 conj in,washed
R5577 T19093 T19092 pobj PBS,in
R5578 T19094 T19078 punct ", ",washed
R5579 T19095 T19096 prep after,purified
R5580 T19096 T19078 advcl purified,washed
R5581 T19097 T19095 pobj which,after
R5582 T19098 T19096 nsubjpass membranes,purified
R5583 T19099 T19096 auxpass were,purified
R5584 T19100 T19096 cc and,purified
R5585 T19101 T19096 conj solubilized,purified
R5586 T19102 T19103 mark as,described
R5587 T19103 T19101 advcl described,solubilized
R5588 T19104 T19103 advmod above,described
R5589 T19105 T19078 punct .,washed
R5590 T19107 T19108 amod Solubilized,membranes
R5591 T19108 T19109 nsubjpass membranes,incubated
R5592 T19110 T19109 auxpass were,incubated
R5593 T19111 T19109 prep with,incubated
R5594 T19112 T19113 nummod 20,μl
R5595 T19113 T19111 pobj μl,with
R5596 T19114 T19113 prep of,μl
R5597 T19115 T19116 amod immobilized,streptavidin
R5598 T19116 T19114 pobj streptavidin,of
R5599 T19117 T19118 punct (,Biotechnology
R5600 T19118 T19116 parataxis Biotechnology,streptavidin
R5601 T19119 T19118 compound Pierce,Biotechnology
R5602 T19120 T19118 punct ),Biotechnology
R5603 T19121 T19109 prep for,incubated
R5604 T19122 T19123 nummod 2,h
R5605 T19123 T19121 pobj h,for
R5606 T19124 T19109 prep at,incubated
R5607 T19125 T19126 nummod 4,°C
R5608 T19126 T19124 pobj °C,at
R5609 T19127 T19109 punct .,incubated
R5610 T19129 T19130 advmod Finally,washed
R5611 T19131 T19132 det the,proteins
R5612 T19132 T19130 nsubjpass proteins,washed
R5613 T19133 T19132 amod precipitated,proteins
R5614 T19134 T19130 auxpass were,washed
R5615 T19135 T19130 prep in,washed
R5616 T19136 T19137 compound RIPA,buffer
R5617 T19137 T19135 pobj buffer,in
R5618 T19138 T19130 cc and,washed
R5619 T19139 T19130 conj boiled,washed
R5620 T19140 T19139 prep in,boiled
R5621 T19141 T19142 nummod 50,μl
R5622 T19142 T19140 pobj μl,in
R5623 T19143 T19142 prep of,μl
R5624 T19144 T19145 compound Laemmli,buffer
R5625 T19145 T19143 pobj buffer,of
R5626 T19146 T19130 punct .,washed
R5627 T19148 T19149 amod Total,cells
R5628 T19149 T19150 nsubj cells,immunoblotting
R5629 T19151 T19149 cc and,cells
R5630 T19152 T19153 det the,precipitates
R5631 T19153 T19149 conj precipitates,cells
R5632 T19154 T19153 amod biotinylated,precipitates
R5633 T19155 T19150 aux were,immunoblotting
R5634 T19156 T19150 advcl using,immunoblotting
R5635 T19157 T19158 det an,antibody
R5636 T19158 T19156 dobj antibody,using
R5637 T19159 T19158 prep to,antibody
R5638 T19160 T19159 pobj AQP2,to
R5639 T19161 T19150 punct .,immunoblotting