Id |
Subject |
Object |
Predicate |
Lexical cue |
T17905 |
0-15 |
NN |
denotes |
Endoglycosidase |
T17906 |
16-25 |
NN |
denotes |
digestion |
T17907 |
25-26 |
. |
denotes |
. |
T17908 |
26-182 |
sentence |
denotes |
Kidney membranes (60 μg) were incubated in 50 mM sodium phosphate (pH 5.5), 0.1% SDS, and 50 mM β-mercaptoethanol, heated to 100 °C for 5 min, then cooled. |
T17909 |
27-33 |
NN |
denotes |
Kidney |
T17910 |
34-43 |
NNS |
denotes |
membranes |
T17911 |
57-66 |
VBN |
denotes |
incubated |
T17912 |
44-45 |
-LRB- |
denotes |
( |
T17913 |
48-50 |
NN |
denotes |
μg |
T17914 |
45-47 |
CD |
denotes |
60 |
T17915 |
50-51 |
-RRB- |
denotes |
) |
T17916 |
52-56 |
VBD |
denotes |
were |
T17917 |
67-69 |
IN |
denotes |
in |
T17918 |
70-72 |
CD |
denotes |
50 |
T17919 |
73-75 |
NN |
denotes |
mM |
T17920 |
83-92 |
NN |
denotes |
phosphate |
T17921 |
76-82 |
NN |
denotes |
sodium |
T17922 |
93-94 |
-LRB- |
denotes |
( |
T17923 |
94-96 |
NN |
denotes |
pH |
T17924 |
97-100 |
CD |
denotes |
5.5 |
T17925 |
100-101 |
-RRB- |
denotes |
) |
T17926 |
101-103 |
, |
denotes |
, |
T17927 |
103-106 |
CD |
denotes |
0.1 |
T17928 |
106-107 |
NN |
denotes |
% |
T17929 |
108-111 |
NN |
denotes |
SDS |
T17930 |
111-113 |
, |
denotes |
, |
T17931 |
113-116 |
CC |
denotes |
and |
T17932 |
117-119 |
CD |
denotes |
50 |
T17933 |
120-122 |
NN |
denotes |
mM |
T17934 |
125-140 |
NN |
denotes |
mercaptoethanol |
T17935 |
123-124 |
NN |
denotes |
β |
T17936 |
124-125 |
HYPH |
denotes |
- |
T17937 |
140-142 |
, |
denotes |
, |
T17938 |
142-148 |
VBN |
denotes |
heated |
T17939 |
149-151 |
IN |
denotes |
to |
T17940 |
152-155 |
CD |
denotes |
100 |
T17941 |
156-158 |
NN |
denotes |
°C |
T17942 |
159-162 |
IN |
denotes |
for |
T17943 |
163-164 |
CD |
denotes |
5 |
T17944 |
165-168 |
NN |
denotes |
min |
T17945 |
168-170 |
, |
denotes |
, |
T17946 |
170-174 |
RB |
denotes |
then |
T17947 |
175-181 |
VBN |
denotes |
cooled |
T17948 |
181-182 |
. |
denotes |
. |
T17949 |
182-270 |
sentence |
denotes |
Endoglycosidase H (0.01 units; Sigma-Aldrich) was added and incubated at 37 °C for 2 h. |
T17950 |
183-198 |
NN |
denotes |
Endoglycosidase |
T17951 |
199-200 |
NN |
denotes |
H |
T17952 |
233-238 |
VBN |
denotes |
added |
T17953 |
201-202 |
-LRB- |
denotes |
( |
T17954 |
220-227 |
NNP |
denotes |
Aldrich |
T17955 |
202-206 |
CD |
denotes |
0.01 |
T17956 |
207-212 |
NNS |
denotes |
units |
T17957 |
212-213 |
: |
denotes |
; |
T17958 |
214-219 |
NNP |
denotes |
Sigma |
T17959 |
219-220 |
HYPH |
denotes |
- |
T17960 |
227-228 |
-RRB- |
denotes |
) |
T17961 |
229-232 |
VBD |
denotes |
was |
T17962 |
239-242 |
CC |
denotes |
and |
T17963 |
243-252 |
VBN |
denotes |
incubated |
T17964 |
253-255 |
IN |
denotes |
at |
T17965 |
256-258 |
CD |
denotes |
37 |
T17966 |
259-261 |
NN |
denotes |
°C |
T17967 |
262-265 |
IN |
denotes |
for |
T17968 |
266-267 |
CD |
denotes |
2 |
T17969 |
268-269 |
NN |
denotes |
h |
T17970 |
269-270 |
. |
denotes |
. |
T17971 |
270-337 |
sentence |
denotes |
The reaction was stopped by boiling the samples in Laemmli buffer. |
T17972 |
271-274 |
DT |
denotes |
The |
T17973 |
275-283 |
NN |
denotes |
reaction |
T17974 |
288-295 |
VBN |
denotes |
stopped |
T17975 |
284-287 |
VBD |
denotes |
was |
T17976 |
296-298 |
IN |
denotes |
by |
T17977 |
299-306 |
VBG |
denotes |
boiling |
T17978 |
307-310 |
DT |
denotes |
the |
T17979 |
311-318 |
NNS |
denotes |
samples |
T17980 |
319-321 |
IN |
denotes |
in |
T17981 |
322-329 |
NNP |
denotes |
Laemmli |
T17982 |
330-336 |
NN |
denotes |
buffer |
T17983 |
336-337 |
. |
denotes |
. |
T17984 |
337-392 |
sentence |
denotes |
Total reactants were immunoblotted as described above. |
T17985 |
338-343 |
JJ |
denotes |
Total |
T17986 |
344-353 |
NNS |
denotes |
reactants |
T17987 |
359-372 |
VBN |
denotes |
immunoblotted |
T17988 |
354-358 |
VBD |
denotes |
were |
T17989 |
373-375 |
IN |
denotes |
as |
T17990 |
376-385 |
VBN |
denotes |
described |
T17991 |
386-391 |
RB |
denotes |
above |
T17992 |
391-392 |
. |
denotes |
. |
R5213 |
T17905 |
T17906 |
compound |
Endoglycosidase,digestion |
R5214 |
T17907 |
T17906 |
punct |
.,digestion |
R5215 |
T17909 |
T17910 |
compound |
Kidney,membranes |
R5216 |
T17910 |
T17911 |
nsubjpass |
membranes,incubated |
R5217 |
T17912 |
T17913 |
punct |
(,μg |
R5218 |
T17913 |
T17910 |
parataxis |
μg,membranes |
R5219 |
T17914 |
T17913 |
nummod |
60,μg |
R5220 |
T17915 |
T17913 |
punct |
),μg |
R5221 |
T17916 |
T17911 |
auxpass |
were,incubated |
R5222 |
T17917 |
T17911 |
prep |
in,incubated |
R5223 |
T17918 |
T17919 |
nummod |
50,mM |
R5224 |
T17919 |
T17920 |
compound |
mM,phosphate |
R5225 |
T17920 |
T17917 |
pobj |
phosphate,in |
R5226 |
T17921 |
T17920 |
compound |
sodium,phosphate |
R5227 |
T17922 |
T17920 |
punct |
(,phosphate |
R5228 |
T17923 |
T17920 |
npadvmod |
pH,phosphate |
R5229 |
T17924 |
T17923 |
nummod |
5.5,pH |
R5230 |
T17925 |
T17920 |
punct |
),phosphate |
R5231 |
T17926 |
T17920 |
punct |
", ",phosphate |
R5232 |
T17927 |
T17928 |
nummod |
0.1,% |
R5233 |
T17928 |
T17929 |
compound |
%,SDS |
R5234 |
T17929 |
T17920 |
appos |
SDS,phosphate |
R5235 |
T17930 |
T17929 |
punct |
", ",SDS |
R5236 |
T17931 |
T17929 |
cc |
and,SDS |
R5237 |
T17932 |
T17933 |
nummod |
50,mM |
R5238 |
T17933 |
T17934 |
compound |
mM,mercaptoethanol |
R5239 |
T17934 |
T17929 |
conj |
mercaptoethanol,SDS |
R5240 |
T17935 |
T17934 |
compound |
β,mercaptoethanol |
R5241 |
T17936 |
T17934 |
punct |
-,mercaptoethanol |
R5242 |
T17937 |
T17911 |
punct |
", ",incubated |
R5243 |
T17938 |
T17911 |
dep |
heated,incubated |
R5244 |
T17939 |
T17938 |
prep |
to,heated |
R5245 |
T17940 |
T17941 |
nummod |
100,°C |
R5246 |
T17941 |
T17939 |
pobj |
°C,to |
R5247 |
T17942 |
T17938 |
prep |
for,heated |
R5248 |
T17943 |
T17944 |
nummod |
5,min |
R5249 |
T17944 |
T17942 |
pobj |
min,for |
R5250 |
T17945 |
T17911 |
punct |
", ",incubated |
R5251 |
T17946 |
T17947 |
advmod |
then,cooled |
R5252 |
T17947 |
T17911 |
dep |
cooled,incubated |
R5253 |
T17948 |
T17911 |
punct |
.,incubated |
R5254 |
T17950 |
T17951 |
compound |
Endoglycosidase,H |
R5255 |
T17951 |
T17952 |
nsubjpass |
H,added |
R5256 |
T17953 |
T17954 |
punct |
(,Aldrich |
R5257 |
T17954 |
T17951 |
parataxis |
Aldrich,H |
R5258 |
T17955 |
T17956 |
nummod |
0.01,units |
R5259 |
T17956 |
T17954 |
dep |
units,Aldrich |
R5260 |
T17957 |
T17954 |
punct |
;,Aldrich |
R5261 |
T17958 |
T17954 |
compound |
Sigma,Aldrich |
R5262 |
T17959 |
T17954 |
punct |
-,Aldrich |
R5263 |
T17960 |
T17954 |
punct |
),Aldrich |
R5264 |
T17961 |
T17952 |
auxpass |
was,added |
R5265 |
T17962 |
T17952 |
cc |
and,added |
R5266 |
T17963 |
T17952 |
conj |
incubated,added |
R5267 |
T17964 |
T17963 |
prep |
at,incubated |
R5268 |
T17965 |
T17966 |
nummod |
37,°C |
R5269 |
T17966 |
T17964 |
pobj |
°C,at |
R5270 |
T17967 |
T17963 |
prep |
for,incubated |
R5271 |
T17968 |
T17969 |
nummod |
2,h |
R5272 |
T17969 |
T17967 |
pobj |
h,for |
R5273 |
T17970 |
T17952 |
punct |
.,added |
R5274 |
T17972 |
T17973 |
det |
The,reaction |
R5275 |
T17973 |
T17974 |
nsubjpass |
reaction,stopped |
R5276 |
T17975 |
T17974 |
auxpass |
was,stopped |
R5277 |
T17976 |
T17974 |
prep |
by,stopped |
R5278 |
T17977 |
T17976 |
pcomp |
boiling,by |
R5279 |
T17978 |
T17979 |
det |
the,samples |
R5280 |
T17979 |
T17977 |
dobj |
samples,boiling |
R5281 |
T17980 |
T17977 |
prep |
in,boiling |
R5282 |
T17981 |
T17982 |
compound |
Laemmli,buffer |
R5283 |
T17982 |
T17980 |
pobj |
buffer,in |
R5284 |
T17983 |
T17974 |
punct |
.,stopped |
R5285 |
T17985 |
T17986 |
amod |
Total,reactants |
R5286 |
T17986 |
T17987 |
nsubjpass |
reactants,immunoblotted |
R5287 |
T17988 |
T17987 |
auxpass |
were,immunoblotted |
R5288 |
T17989 |
T17990 |
mark |
as,described |
R5289 |
T17990 |
T17987 |
advcl |
described,immunoblotted |
R5290 |
T17991 |
T17990 |
advmod |
above,described |
R5291 |
T17992 |
T17987 |
punct |
.,immunoblotted |