PMC:1189073 / 34698-35134
Annnotations
craft-ca-core-ex-dev
Below, discontinuous spans are shown in the chain model. You can change it to the bag model.
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T17256 | 6-11 | NCBITaxon:10088 | denotes | mouse |
T17257 | 12-19 | UBERON:0002113 | denotes | kidneys |
T17258 | 46-50 | CHEBI:9754 | denotes | Tris |
T17259 | 68-75 | CHEBI:17992 | denotes | sucrose |
T17260 | 86-90 | CHEBI_EXT:EDTA | denotes | EDTA |
T17261 | 102-121 | CHEBI_GO_EXT:peptidase_or_protease_or_proteinase_inhibitor | denotes | protease inhibitors |
T17262 | 330-339 | GO:0016020 | denotes | membranes |
T17263 | 369-375 | CHEBI_CHMO_EXT:buffer_solution | denotes | buffer |
T17264 | 381-388 | CHEBI_PR_EXT:protein | denotes | protein |
craft-ca-core-dev
Below, discontinuous spans are shown in the chain model. You can change it to the bag model.
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T17249 | 6-11 | NCBITaxon:10088 | denotes | mouse |
T17250 | 12-19 | UBERON:0002113 | denotes | kidneys |
T17251 | 46-50 | CHEBI:9754 | denotes | Tris |
T17252 | 68-75 | CHEBI:17992 | denotes | sucrose |
T17253 | 330-339 | GO:0016020 | denotes | membranes |
craft-sa-dev
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T17314 | 185-195 | NN | denotes | homogenate |
T17270 | 0-5 | JJ | denotes | Whole |
T17271 | 12-19 | NNS | denotes | kidneys |
T17272 | 6-11 | NN | denotes | mouse |
T17273 | 25-36 | VBN | denotes | homogenized |
T17274 | 20-24 | VBD | denotes | were |
T17275 | 37-39 | IN | denotes | in |
T17276 | 40-42 | CD | denotes | 10 |
T17277 | 43-45 | NN | denotes | mM |
T17278 | 46-50 | NN | denotes | Tris |
T17279 | 51-52 | -LRB- | denotes | ( |
T17280 | 52-54 | NN | denotes | pH |
T17281 | 55-58 | CD | denotes | 7.4 |
T17282 | 58-59 | -RRB- | denotes | ) |
T17283 | 59-61 | , | denotes | , |
T17284 | 61-64 | CD | denotes | 350 |
T17285 | 65-67 | NN | denotes | mM |
T17286 | 68-75 | NN | denotes | sucrose |
T17287 | 75-77 | , | denotes | , |
T17288 | 77-80 | CC | denotes | and |
T17289 | 81-82 | CD | denotes | 5 |
T17290 | 83-85 | NN | denotes | mM |
T17291 | 86-90 | NN | denotes | EDTA |
T17292 | 91-101 | VBG | denotes | containing |
T17293 | 102-110 | NN | denotes | protease |
T17294 | 111-121 | NNS | denotes | inhibitors |
T17295 | 122-123 | -LRB- | denotes | ( |
T17296 | 139-140 | NN | denotes | P |
T17297 | 123-128 | NNP | denotes | Sigma |
T17298 | 129-136 | NNP | denotes | Aldrich |
T17299 | 128-129 | HYPH | denotes | - |
T17300 | 136-138 | , | denotes | , |
T17301 | 138-139 | SYM | denotes | # |
T17302 | 140-141 | HYPH | denotes | - |
T17303 | 141-145 | CD | denotes | 8340 |
T17304 | 145-146 | -RRB- | denotes | ) |
T17305 | 147-149 | IN | denotes | in |
T17306 | 150-151 | DT | denotes | a |
T17307 | 168-179 | NN | denotes | homogenizer |
T17308 | 152-158 | NNP | denotes | Potter |
T17309 | 159-167 | NNP | denotes | Elvehjem |
T17310 | 158-159 | HYPH | denotes | - |
T17311 | 179-180 | . | denotes | . |
T17312 | 180-320 | sentence | denotes | The homogenate was centrifuged at 2,000 g for 10 min and the supernatant was subjected to ultracentrifugation at 100,000 g for 1 h at 4 °C. |
T17313 | 181-184 | DT | denotes | The |
T17315 | 200-211 | VBN | denotes | centrifuged |
T17316 | 196-199 | VBD | denotes | was |
T17317 | 212-214 | IN | denotes | at |
T17318 | 215-220 | CD | denotes | 2,000 |
T17319 | 221-222 | NN | denotes | g |
T17320 | 223-226 | IN | denotes | for |
T17321 | 227-229 | CD | denotes | 10 |
T17322 | 230-233 | NN | denotes | min |
T17323 | 234-237 | CC | denotes | and |
T17324 | 238-241 | DT | denotes | the |
T17325 | 242-253 | NN | denotes | supernatant |
T17326 | 258-267 | VBN | denotes | subjected |
T17327 | 254-257 | VBD | denotes | was |
T17328 | 268-270 | IN | denotes | to |
T17329 | 271-290 | NN | denotes | ultracentrifugation |
T17330 | 291-293 | IN | denotes | at |
T17331 | 294-301 | CD | denotes | 100,000 |
T17332 | 302-303 | NN | denotes | g |
T17333 | 304-307 | IN | denotes | for |
T17334 | 308-309 | CD | denotes | 1 |
T17335 | 310-311 | NN | denotes | h |
T17336 | 312-314 | IN | denotes | at |
T17337 | 315-316 | CD | denotes | 4 |
T17338 | 317-319 | NN | denotes | °C |
T17339 | 319-320 | . | denotes | . |
T17340 | 320-436 | sentence | denotes | Pelleted membranes were resuspended in the same buffer, and protein concentration was determined by Bradford assay. |
T17341 | 321-329 | VBN | denotes | Pelleted |
T17342 | 330-339 | NNS | denotes | membranes |
T17343 | 345-356 | VBN | denotes | resuspended |
T17344 | 340-344 | VBD | denotes | were |
T17345 | 357-359 | IN | denotes | in |
T17346 | 360-363 | DT | denotes | the |
T17347 | 369-375 | NN | denotes | buffer |
T17348 | 364-368 | JJ | denotes | same |
T17349 | 375-377 | , | denotes | , |
T17350 | 377-380 | CC | denotes | and |
T17351 | 381-388 | NN | denotes | protein |
T17352 | 389-402 | NN | denotes | concentration |
T17353 | 407-417 | VBN | denotes | determined |
T17354 | 403-406 | VBD | denotes | was |
T17355 | 418-420 | IN | denotes | by |
T17356 | 421-429 | NNP | denotes | Bradford |
T17357 | 430-435 | NN | denotes | assay |
T17358 | 435-436 | . | denotes | . |
R5014 | T17270 | T17271 | amod | Whole,kidneys |
R5015 | T17271 | T17273 | nsubjpass | kidneys,homogenized |
R5016 | T17272 | T17271 | compound | mouse,kidneys |
R5017 | T17274 | T17273 | auxpass | were,homogenized |
R5018 | T17275 | T17273 | prep | in,homogenized |
R5019 | T17276 | T17277 | nummod | 10,mM |
R5020 | T17277 | T17278 | compound | mM,Tris |
R5021 | T17278 | T17275 | pobj | Tris,in |
R5022 | T17279 | T17278 | punct | (,Tris |
R5023 | T17280 | T17278 | npadvmod | pH,Tris |
R5024 | T17281 | T17280 | nummod | 7.4,pH |
R5025 | T17282 | T17278 | punct | ),Tris |
R5026 | T17283 | T17278 | punct | ", ",Tris |
R5027 | T17284 | T17285 | nummod | 350,mM |
R5028 | T17285 | T17286 | compound | mM,sucrose |
R5029 | T17286 | T17278 | conj | sucrose,Tris |
R5030 | T17287 | T17286 | punct | ", ",sucrose |
R5031 | T17288 | T17286 | cc | and,sucrose |
R5032 | T17289 | T17290 | nummod | 5,mM |
R5033 | T17290 | T17291 | compound | mM,EDTA |
R5034 | T17291 | T17286 | conj | EDTA,sucrose |
R5035 | T17292 | T17291 | acl | containing,EDTA |
R5036 | T17293 | T17294 | compound | protease,inhibitors |
R5037 | T17294 | T17292 | dobj | inhibitors,containing |
R5038 | T17295 | T17296 | punct | (,P |
R5039 | T17296 | T17294 | parataxis | P,inhibitors |
R5040 | T17297 | T17298 | compound | Sigma,Aldrich |
R5041 | T17298 | T17296 | dep | Aldrich,P |
R5042 | T17299 | T17298 | punct | -,Aldrich |
R5043 | T17300 | T17296 | punct | ", ",P |
R5044 | T17301 | T17296 | punct | #,P |
R5045 | T17302 | T17296 | punct | -,P |
R5046 | T17303 | T17296 | nummod | 8340,P |
R5047 | T17304 | T17296 | punct | ),P |
R5048 | T17305 | T17273 | prep | in,homogenized |
R5049 | T17306 | T17307 | det | a,homogenizer |
R5050 | T17307 | T17305 | pobj | homogenizer,in |
R5051 | T17308 | T17309 | compound | Potter,Elvehjem |
R5052 | T17309 | T17307 | compound | Elvehjem,homogenizer |
R5053 | T17310 | T17309 | punct | -,Elvehjem |
R5054 | T17311 | T17273 | punct | .,homogenized |
R5055 | T17313 | T17314 | det | The,homogenate |
R5056 | T17314 | T17315 | nsubjpass | homogenate,centrifuged |
R5057 | T17316 | T17315 | auxpass | was,centrifuged |
R5058 | T17317 | T17315 | prep | at,centrifuged |
R5059 | T17318 | T17319 | nummod | "2,000",g |
R5060 | T17319 | T17317 | pobj | g,at |
R5061 | T17320 | T17315 | prep | for,centrifuged |
R5062 | T17321 | T17322 | nummod | 10,min |
R5063 | T17322 | T17320 | pobj | min,for |
R5064 | T17323 | T17315 | cc | and,centrifuged |
R5065 | T17324 | T17325 | det | the,supernatant |
R5066 | T17325 | T17326 | nsubjpass | supernatant,subjected |
R5067 | T17326 | T17315 | conj | subjected,centrifuged |
R5068 | T17327 | T17326 | auxpass | was,subjected |
R5069 | T17328 | T17326 | prep | to,subjected |
R5070 | T17329 | T17328 | pobj | ultracentrifugation,to |
R5071 | T17330 | T17329 | prep | at,ultracentrifugation |
R5072 | T17331 | T17332 | nummod | "100,000",g |
R5073 | T17332 | T17330 | pobj | g,at |
R5074 | T17333 | T17326 | prep | for,subjected |
R5075 | T17334 | T17335 | nummod | 1,h |
R5076 | T17335 | T17333 | pobj | h,for |
R5077 | T17336 | T17326 | prep | at,subjected |
R5078 | T17337 | T17338 | nummod | 4,°C |
R5079 | T17338 | T17336 | pobj | °C,at |
R5080 | T17339 | T17326 | punct | .,subjected |
R5081 | T17341 | T17342 | amod | Pelleted,membranes |
R5082 | T17342 | T17343 | nsubjpass | membranes,resuspended |
R5083 | T17344 | T17343 | auxpass | were,resuspended |
R5084 | T17345 | T17343 | prep | in,resuspended |
R5085 | T17346 | T17347 | det | the,buffer |
R5086 | T17347 | T17345 | pobj | buffer,in |
R5087 | T17348 | T17347 | amod | same,buffer |
R5088 | T17349 | T17343 | punct | ", ",resuspended |
R5089 | T17350 | T17343 | cc | and,resuspended |
R5090 | T17351 | T17352 | compound | protein,concentration |
R5091 | T17352 | T17353 | nsubjpass | concentration,determined |
R5092 | T17353 | T17343 | conj | determined,resuspended |
R5093 | T17354 | T17353 | auxpass | was,determined |
R5094 | T17355 | T17353 | prep | by,determined |
R5095 | T17356 | T17357 | compound | Bradford,assay |
R5096 | T17357 | T17355 | pobj | assay,by |
R5097 | T17358 | T17353 | punct | .,determined |