> top > docs > PMC:1189073 > spans > 25764-31716 > annotations

PMC:1189073 / 25764-31716 JSONTXT

Annnotations TAB JSON ListView MergeView

craft-ca-core-ex-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T13940 11-15 PR_EXT:000004182 denotes Aqp2
T13941 26-30 NCBITaxon:10088 denotes mice
T13942 46-50 GO:0040007 denotes grow
T13943 55-64 GO:0000003 denotes reproduce
T13944 145-150 UBERON:0001088 denotes urine
T13945 173-178 UBERON:0001088 denotes urine
T13946 190-195 CHEBI:15377 denotes water
T13947 196-202 GO:0007631 denotes intake
T13948 231-236 NCBITaxon:10088 denotes mouse
T13949 277-283 NCBITaxon:9606 denotes humans
T13950 302-311 SO_EXT:sequence_alteration_entity_or_process denotes mutations
T13951 315-320 PR_EXT:000001683 denotes Avpr2
T13952 324-328 PR_EXT:000004182 denotes Aqp2
T13953 346-347 GO:0000805 denotes X
T13954 355-360 PR_EXT:000001683 denotes Avpr2
T13955 361-365 SO_EXT:0000704 denotes gene
T13956 369-373 NCBITaxon:10088 denotes mice
T13957 409-413 PATO_UBERON_EXT:male_or_bearer_of_maleness denotes male
T13958 426-434 PATO_UBERON_EXT:neonate_or_newborn denotes neonates
T13959 479-485 UBERON:0007023 denotes adults
T13960 493-497 NCBITaxon:10088 denotes mice
T13961 498-502 GO:0016265 denotes died
T13962 518-523 GO:0007567 denotes birth
T13963 529-534 UBERON:0007023 denotes adult
T13964 548-555 PATO_UBERON_EXT:female_or_bearer_of_femaleness denotes females
T13965 596-603 UBERON:0001088 denotes urinary
T13966 615-620 CHEBI:15377 denotes water
T13967 621-627 GO:0007631 denotes intake
T13968 642-647 UBERON:0001088 denotes urine
T13969 676-681 NCBITaxon:10088 denotes mouse
T13970 682-686 PR_EXT:000004182 denotes Aqp2
T13971 687-691 SO_EXT:0000704 denotes gene
T13972 731-736 NCBITaxon:9606 denotes human
T13973 753-761 SO_EXT:sequence_alteration_entity_or_process denotes mutation
T13974 806-810 NCBITaxon:10088 denotes mice
T13975 825-830 UBERON:0001088 denotes urine
T13976 881-886 GO:0016265 denotes death
T13977 902-907 GO:0007567 denotes birth
T13978 920-924 PR_EXT:000004182 denotes AQP2
T13979 936-944 GO_PATO_EXT:biological_localization_process_or_quality denotes localize
T13980 978-983 CL_GO_EXT:cell denotes cells
T13981 1006-1021 UBERON:0001232 denotes collecting duct
T13982 1069-1078 CHEBI_EXT:polyatomic_entity_or_group denotes molecular
T13983 1104-1108 NCBITaxon:10088 denotes mice
T13984 1148-1152 PR_EXT:000004182 denotes Aqp2
T13985 1169-1173 SO_EXT:0000704 denotes gene
T13986 1198-1202 NCBITaxon:10088 denotes mice
T13987 1229-1233 PR_EXT:000004182 denotes AQP2
T13988 1264-1269 CHEBI:15377 denotes water
T13989 1264-1282 GO:0006833 denotes water transporting
T13990 1309-1312 CHEBI_PR_EXT:arginine_vasopressin denotes AVP
T13991 1338-1343 CHEBI:15377 denotes water
T13992 1379-1383 PR_EXT:000004182 denotes AQP2
T13993 1404-1410 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T13994 1411-1418 NCBITaxon:33208 denotes animals
T13995 1435-1443 GO_EXT:reaction_or_response denotes response
T13996 1447-1452 CHEBI:4450 denotes dDAVP
T13997 1463-1468 CHEBI:4450 denotes dDAVP
T13998 1480-1485 UBERON:0001088 denotes urine
T13999 1534-1540 UBERON:0002113 denotes kidney
T14000 1552-1556 PR_EXT:000004182 denotes AQP2
T14001 1584-1593 GO_EXT:biological_routing_or_transport denotes transport
T14002 1594-1599 CHEBI:15377 denotes water
T14003 1621-1629 GO_PATO_EXT:biological_localization_process_or_quality denotes localize
T14004 1637-1643 GO:0045177 denotes apical
T14005 1644-1648 CL_GO_EXT:cell denotes cell
T14006 1644-1656 GO:0009986 denotes cell surface
T14007 1663-1668 CHEBI:4450 denotes dDAVP
T14008 1706-1710 PR_EXT:000004182 denotes AQP2
T14009 1768-1774 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T14010 1775-1782 CHEBI_PR_EXT:protein denotes protein
T14011 1801-1805 CL_GO_EXT:cell denotes cell
T14012 1801-1813 GO:0009986 denotes cell surface
T14013 1819-1826 GO:0009986 denotes surface
T14014 1827-1840 MOP:0000093 denotes biotinylation
T14015 1881-1887 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T14016 1888-1895 CHEBI_PR_EXT:protein denotes protein
T14017 1908-1915 GO:0009986 denotes surface
T14018 2007-2014 CHEBI_PR_EXT:protein denotes protein
T14019 2093-2099 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T14020 2100-2107 CHEBI_PR_EXT:protein denotes protein
T14021 2133-2135 GO:0005783 denotes ER
T14022 2182-2191 SO_EXT:wild_type_entity_or_quality denotes wild-type
T14023 2193-2199 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T14024 2200-2207 CHEBI_PR_EXT:protein denotes protein
T14025 2232-2239 CHEBI:37684 denotes mannose
T14026 2246-2258 GO_MOP_EXT:glycosylation denotes glycosylated
T14027 2293-2305 GO_MOP_EXT:glycosylation denotes glycosylated
T14028 2327-2339 GO_MOP_EXT:glycosylation denotes glycosylated
T14029 2410-2417 CHEBI_PR_EXT:protein denotes protein
T14030 2456-2462 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T14031 2463-2470 CHEBI_PR_EXT:protein denotes protein
T14032 2474-2485 GO_EXT:biological_routing_or_transport denotes transported
T14033 2497-2499 GO:0005783 denotes ER
T14034 2540-2552 GO_PATO_EXT:biological_localization_process_or_quality denotes localization
T14035 2556-2560 PR_EXT:000004182 denotes AQP2
T14036 2576-2578 GO:0005783 denotes ER
T14037 2579-2586 CHEBI_PR_EXT:protein denotes protein
T14038 2587-2595 PR_EXT:000005005 denotes calnexin
T14039 2599-2610 GO:0009294 denotes transfected
T14040 2616-2621 CL_GO_EXT:cell denotes cells
T14041 2652-2658 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T14042 2659-2666 CHEBI_PR_EXT:protein denotes protein
T14043 2685-2687 GO:0005783 denotes ER
T14044 2719-2721 GO:0005783 denotes ER
T14045 2734-2742 GO_EXT:reaction_or_response denotes response
T14046 2746-2751 CHEBI:4450 denotes dDAVP
T14047 2784-2796 GO_MOP_EXT:glycosylation denotes glycosylated
T14048 2797-2804 CHEBI_PR_EXT:protein denotes protein
T14049 2808-2814 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T14050 2815-2822 NCBITaxon:33208 denotes animals
T14051 2832-2844 _FRAGMENT denotes transport of
T14052 2866-2873 GO:0015031 denotes protein
T14053 2859-2865 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T14054 2866-2873 CHEBI_PR_EXT:protein denotes protein
T14055 2885-2887 GO:0005783 denotes ER
T14056 2896-2901 CL_GO_EXT:cell denotes cells
T14057 2942-2946 PR_EXT:000004182 denotes AQP2
T14058 2956-2963 GO:0006457 denotes folding
T14059 3012-3017 CHEBI:15377 denotes water
T14060 3012-3030 GO:0006833 denotes water transporting
T14061 3110-3116 GO_EXT:biological_growth_entity_or_process denotes growth
T14062 3120-3126 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T14063 3127-3134 NCBITaxon:33208 denotes animals
T14064 3170-3172 GO:0005783 denotes ER
T14065 3189-3193 PR_EXT:000004182 denotes AQP2
T14066 3231-3238 CHEBI:37684 denotes mannose
T14067 3239-3251 GO_MOP_EXT:glycosylation denotes glycosylated
T14068 3267-3274 CHEBI:37684 denotes mannose
T14069 3280-3295 CHEBI:50699 denotes oligosaccharide
T14070 3312-3314 GO:0005783 denotes ER
T14071 3328-3336 SO_EXT:sequence_alteration_process denotes modified
T14072 3359-3374 GO:0005794 denotes Golgi apparatus
T14073 3406-3413 CHEBI:37684 denotes mannose
T14074 3414-3426 GO_MOP_EXT:glycosylation denotes glycosylated
T14075 3435-3439 PR_EXT:000004182 denotes AQP2
T14076 3495-3497 GO:0005783 denotes ER
T14077 3514-3528 CHEBI:50699 denotes oligosaccharyl
T14078 3514-3540 GO_EXT:0004576 denotes oligosaccharyl transferase
T14079 3557-3569 GO_PATO_EXT:biological_localization_process_or_quality denotes localization
T14080 3573-3577 PR_EXT:000004182 denotes AQP2
T14081 3615-3621 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T14082 3622-3626 NCBITaxon:10088 denotes mice
T14083 3677-3681 NCBITaxon:10088 denotes mice
T14084 3712-3727 GO_PATO_EXT:physiological_process_or_quality denotes Physiologically
T14085 3742-3746 NCBITaxon:10088 denotes mice
T14086 3817-3826 SO_EXT:wild_type_entity_or_quality denotes wild type
T14087 3848-3855 UBERON:0002113 denotes kidneys
T14088 3913-3922 SO_EXT:wild_type_entity_or_quality denotes wild-type
T14089 3923-3930 CHEBI_PR_EXT:protein denotes protein
T14090 4003-4005 GO:0005783 denotes ER
T14091 4015-4021 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T14092 4022-4029 CHEBI_PR_EXT:protein denotes protein
T14093 4060-4064 PR_EXT:000004182 denotes Aqp2
T14094 4070-4071 SO_EXT:normal_or_wild_type_or_present denotes +
T14095 4072-4079 NCBITaxon:33208 denotes animals
T14096 4098-4104 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T14097 4105-4112 CHEBI_PR_EXT:protein denotes protein
T14098 4122-4129 SO_EXT:sequence_rescue_process denotes rescued
T14099 4137-4146 SO_EXT:wild_type_entity_or_quality denotes wild-type
T14100 4147-4154 CHEBI_PR_EXT:protein denotes protein
T14101 4223-4229 SO_EXT:0001023 denotes allele
T14102 4233-4237 PR_EXT:000004182 denotes Aqp2
T14103 4264-4275 GO_EXT:biological_movement_or_translocation_process denotes translocate
T14104 4281-4290 GO:0010467 denotes expressed
T14105 4309-4314 CL_GO_EXT:cell denotes cells
T14106 4344-4353 SO_EXT:wild_type_entity_or_quality denotes wild-type
T14107 4354-4361 CHEBI_PR_EXT:protein denotes protein
T14108 4366-4375 GO_PATO_EXT:biological_localization_process_or_quality denotes localizes
T14109 4433-4437 PR_EXT:000004182 denotes Aqp2
T14110 4443-4444 SO_EXT:normal_or_wild_type_or_present denotes +
T14111 4445-4449 NCBITaxon:10088 denotes mice
T14112 4471-4475 PR_EXT:000004182 denotes AQP2
T14113 4500-4509 SO_EXT:wild_type_entity_or_quality denotes wild-type
T14114 4510-4514 PR_EXT:000004182 denotes AQP2
T14115 4539-4548 GO:0010467 denotes expressed
T14116 4554-4563 SO_EXT:wild_type_entity_or_quality denotes wild-type
T14117 4564-4571 CHEBI_PR_EXT:protein denotes protein
T14118 4573-4577 PR_EXT:000004182 denotes AQP2
T14119 4598-4602 CL_GO_EXT:cell denotes cell
T14120 4598-4610 GO:0009986 denotes cell surface
T14121 4701-4707 SO_EXT:0001023 denotes allele
T14122 4709-4717 SO_EXT:sequence_coding_function denotes encoding
T14123 4718-4722 PR_EXT:000004182 denotes AQP2
T14124 4760-4769 SO_EXT:wild_type_entity_or_quality denotes wild-type
T14125 4770-4774 PR_EXT:000004182 denotes AQP2
T14126 4798-4802 PR_EXT:000004182 denotes AQP2
T14127 4832-4841 SO_EXT:wild_type_entity_or_quality denotes wild-type
T14128 4842-4849 CHEBI_PR_EXT:protein denotes protein
T14129 4879-4888 GO_EXT:tetrameric_macromolecular_complex denotes tetramers
T14130 4907-4916 SO_EXT:sequence_rescue_process denotes rescuable
T14131 4917-4923 SO_EXT:0001023 denotes allele
T14132 4971-4978 UBERON:0002113 denotes kidneys
T14133 4993-4997 PR_EXT:000004182 denotes Aqp2
T14134 5009-5013 NCBITaxon:10088 denotes mice
T14135 5029-5035 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T14136 5036-5046 GO:0010467 denotes expressing
T14137 5047-5062 UBERON:0001232 denotes collecting duct
T14138 5047-5068 CL:1001225 denotes collecting duct cells
T14139 5063-5068 CL_GO_EXT:cell denotes cells
T14140 5085-5090 CHEBI:15377 denotes water
T14141 5141-5163 GO:0016324 denotes apical plasma membrane
T14142 5167-5175 GO_EXT:reaction_or_response denotes response
T14143 5179-5184 CHEBI:4450 denotes dDAVP
T14144 5297-5301 PR_EXT:000004182 denotes Aqp2
T14145 5302-5311 SO_EXT:sequence_alteration_entity_or_process denotes mutations
T14146 5313-5325 GO:0009294 denotes Transfection
T14147 5336-5341 CL_GO_EXT:cell denotes cells
T14148 5360-5364 PR_EXT:000004182 denotes Aqp2
T14149 5365-5374 SO_EXT:sequence_alteration_entity_or_process denotes mutations
T14150 5402-5407 NCBITaxon:9606 denotes human
T14151 5408-5415 SO_EXT:0001023 denotes alleles
T14152 5431-5442 GO_UBERON_EXT:cellular_component_or_cell_part denotes subcellular
T14153 5434-5442 CL_GO_EXT:cell denotes cellular
T14154 5443-5455 GO_PATO_EXT:biological_localization_process_or_quality denotes localization
T14155 5496-5507 GO_EXT:biological_movement_or_translocation_process denotes translocate
T14156 5515-5530 GO:0005886 denotes plasma membrane
T14157 5541-5548 GO:0006457 denotes folding
T14158 5567-5569 GO:0005783 denotes ER
T14159 5586-5597 GO_EXT:biological_movement_or_translocation_process denotes translocate
T14160 5601-5609 GO_EXT:reaction_or_response denotes response
T14161 5613-5618 CHEBI:4450 denotes dDAVP
T14162 5654-5663 GO:0030849 denotes autosomal
T14163 5756-5761 NCBITaxon:9606 denotes human
T14164 5772-5777 NCBITaxon:10088 denotes mouse
T14165 5803-5807 PR_EXT:000004182 denotes Aqp2
T14166 5808-5814 SO_EXT:0001023 denotes allele
T14167 5827-5834 SO_EXT:sequence_rescue_process denotes rescued
T14168 5889-5893 SO_EXT:0000704 denotes gene
T14169 5927-5938 GO_UBERON_EXT:cellular_component_or_cell_part denotes subcellular
T14170 5930-5938 CL_GO_EXT:cell denotes cellular
T14171 5939-5951 GO_PATO_EXT:biological_localization_process_or_quality denotes localization
R3377 T14052 T14051 _lexicallyChainedTo protein,transport of

craft-ca-core-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T13804 11-15 PR:000004182 denotes Aqp2
T13805 26-30 NCBITaxon:10088 denotes mice
T13806 46-50 GO:0040007 denotes grow
T13807 55-64 GO:0000003 denotes reproduce
T13808 145-150 UBERON:0001088 denotes urine
T13809 173-178 UBERON:0001088 denotes urine
T13810 190-195 CHEBI:15377 denotes water
T13811 196-202 GO:0007631 denotes intake
T13812 231-236 NCBITaxon:10088 denotes mouse
T13813 277-283 NCBITaxon:9606 denotes humans
T13814 315-320 PR:000001683 denotes Avpr2
T13815 324-328 PR:000004182 denotes Aqp2
T13816 346-347 GO:0000805 denotes X
T13817 355-360 PR:000001683 denotes Avpr2
T13818 361-365 SO:0000704 denotes gene
T13819 369-373 NCBITaxon:10088 denotes mice
T13820 479-485 UBERON:0007023 denotes adults
T13821 493-497 NCBITaxon:10088 denotes mice
T13822 498-502 GO:0016265 denotes died
T13823 518-523 GO:0007567 denotes birth
T13824 529-534 UBERON:0007023 denotes adult
T13825 596-603 UBERON:0001088 denotes urinary
T13826 615-620 CHEBI:15377 denotes water
T13827 621-627 GO:0007631 denotes intake
T13828 642-647 UBERON:0001088 denotes urine
T13829 676-681 NCBITaxon:10088 denotes mouse
T13830 682-686 PR:000004182 denotes Aqp2
T13831 687-691 SO:0000704 denotes gene
T13832 731-736 NCBITaxon:9606 denotes human
T13833 806-810 NCBITaxon:10088 denotes mice
T13834 825-830 UBERON:0001088 denotes urine
T13835 881-886 GO:0016265 denotes death
T13836 902-907 GO:0007567 denotes birth
T13837 920-924 PR:000004182 denotes AQP2
T13838 1006-1021 UBERON:0001232 denotes collecting duct
T13839 1104-1108 NCBITaxon:10088 denotes mice
T13840 1148-1152 PR:000004182 denotes Aqp2
T13841 1169-1173 SO:0000704 denotes gene
T13842 1198-1202 NCBITaxon:10088 denotes mice
T13843 1229-1233 PR:000004182 denotes AQP2
T13844 1264-1269 CHEBI:15377 denotes water
T13845 1264-1282 GO:0006833 denotes water transporting
T13846 1338-1343 CHEBI:15377 denotes water
T13847 1379-1383 PR:000004182 denotes AQP2
T13848 1411-1418 NCBITaxon:33208 denotes animals
T13849 1447-1452 CHEBI:4450 denotes dDAVP
T13850 1463-1468 CHEBI:4450 denotes dDAVP
T13851 1480-1485 UBERON:0001088 denotes urine
T13852 1534-1540 UBERON:0002113 denotes kidney
T13853 1552-1556 PR:000004182 denotes AQP2
T13854 1594-1599 CHEBI:15377 denotes water
T13855 1637-1643 GO:0045177 denotes apical
T13856 1644-1656 GO:0009986 denotes cell surface
T13857 1663-1668 CHEBI:4450 denotes dDAVP
T13858 1706-1710 PR:000004182 denotes AQP2
T13859 1801-1813 GO:0009986 denotes cell surface
T13860 1819-1826 GO:0009986 denotes surface
T13861 1827-1840 MOP:0000093 denotes biotinylation
T13862 1908-1915 GO:0009986 denotes surface
T13863 2133-2135 GO:0005783 denotes ER
T13864 2232-2239 CHEBI:37684 denotes mannose
T13865 2497-2499 GO:0005783 denotes ER
T13866 2556-2560 PR:000004182 denotes AQP2
T13867 2576-2578 GO:0005783 denotes ER
T13868 2587-2595 PR:000005005 denotes calnexin
T13869 2599-2610 GO:0009294 denotes transfected
T13870 2685-2687 GO:0005783 denotes ER
T13871 2719-2721 GO:0005783 denotes ER
T13872 2746-2751 CHEBI:4450 denotes dDAVP
T13873 2815-2822 NCBITaxon:33208 denotes animals
T13874 2832-2844 _FRAGMENT denotes transport of
T13875 2866-2873 GO:0015031 denotes protein
T13876 2885-2887 GO:0005783 denotes ER
T13877 2942-2946 PR:000004182 denotes AQP2
T13878 2956-2963 GO:0006457 denotes folding
T13879 3012-3017 CHEBI:15377 denotes water
T13880 3012-3030 GO:0006833 denotes water transporting
T13881 3127-3134 NCBITaxon:33208 denotes animals
T13882 3170-3172 GO:0005783 denotes ER
T13883 3189-3193 PR:000004182 denotes AQP2
T13884 3231-3238 CHEBI:37684 denotes mannose
T13885 3267-3274 CHEBI:37684 denotes mannose
T13886 3280-3295 CHEBI:50699 denotes oligosaccharide
T13887 3312-3314 GO:0005783 denotes ER
T13888 3359-3374 GO:0005794 denotes Golgi apparatus
T13889 3406-3413 CHEBI:37684 denotes mannose
T13890 3435-3439 PR:000004182 denotes AQP2
T13891 3495-3497 GO:0005783 denotes ER
T13892 3514-3528 CHEBI:50699 denotes oligosaccharyl
T13893 3573-3577 PR:000004182 denotes AQP2
T13894 3622-3626 NCBITaxon:10088 denotes mice
T13895 3677-3681 NCBITaxon:10088 denotes mice
T13896 3742-3746 NCBITaxon:10088 denotes mice
T13897 3848-3855 UBERON:0002113 denotes kidneys
T13898 4003-4005 GO:0005783 denotes ER
T13899 4060-4064 PR:000004182 denotes Aqp2
T13900 4072-4079 NCBITaxon:33208 denotes animals
T13901 4223-4229 SO:0001023 denotes allele
T13902 4233-4237 PR:000004182 denotes Aqp2
T13903 4281-4290 GO:0010467 denotes expressed
T13904 4433-4437 PR:000004182 denotes Aqp2
T13905 4445-4449 NCBITaxon:10088 denotes mice
T13906 4471-4475 PR:000004182 denotes AQP2
T13907 4510-4514 PR:000004182 denotes AQP2
T13908 4539-4548 GO:0010467 denotes expressed
T13909 4573-4577 PR:000004182 denotes AQP2
T13910 4598-4610 GO:0009986 denotes cell surface
T13911 4701-4707 SO:0001023 denotes allele
T13912 4718-4722 PR:000004182 denotes AQP2
T13913 4770-4774 PR:000004182 denotes AQP2
T13914 4798-4802 PR:000004182 denotes AQP2
T13915 4917-4923 SO:0001023 denotes allele
T13916 4971-4978 UBERON:0002113 denotes kidneys
T13917 4993-4997 PR:000004182 denotes Aqp2
T13918 5009-5013 NCBITaxon:10088 denotes mice
T13919 5036-5046 GO:0010467 denotes expressing
T13920 5047-5062 UBERON:0001232 denotes collecting duct
T13921 5047-5068 CL:1001225 denotes collecting duct cells
T13922 5085-5090 CHEBI:15377 denotes water
T13923 5141-5163 GO:0016324 denotes apical plasma membrane
T13924 5179-5184 CHEBI:4450 denotes dDAVP
T13925 5297-5301 PR:000004182 denotes Aqp2
T13926 5313-5325 GO:0009294 denotes Transfection
T13927 5360-5364 PR:000004182 denotes Aqp2
T13928 5402-5407 NCBITaxon:9606 denotes human
T13929 5408-5415 SO:0001023 denotes alleles
T13930 5515-5530 GO:0005886 denotes plasma membrane
T13931 5541-5548 GO:0006457 denotes folding
T13932 5567-5569 GO:0005783 denotes ER
T13933 5613-5618 CHEBI:4450 denotes dDAVP
T13934 5654-5663 GO:0030849 denotes autosomal
T13935 5756-5761 NCBITaxon:9606 denotes human
T13936 5772-5777 NCBITaxon:10088 denotes mouse
T13937 5803-5807 PR:000004182 denotes Aqp2
T13938 5808-5814 SO:0001023 denotes allele
T13939 5889-5893 SO:0000704 denotes gene
R3376 T13875 T13874 _lexicallyChainedTo protein,transport of

craft-sa-dev

Id Subject Object Predicate Lexical cue
T14172 11-19 NN denotes Aqp2F204
T14173 20-25 NN denotes F204V
T14174 19-20 HYPH denotes /
T14175 26-30 NNS denotes mice
T14176 31-34 VBP denotes are
T14177 35-41 JJ denotes viable
T14178 42-45 CC denotes and
T14179 46-50 VBP denotes grow
T14180 51-54 CC denotes and
T14181 55-64 VBP denotes reproduce
T14182 65-73 RB denotes normally
T14183 73-74 . denotes .
T14184 74-273 sentence denotes They are, however, severely defective in their ability to concentrate urine, leading to increased urine output and water intake, thus making them the first mouse model of NDI to survive to maturity.
T14185 75-79 PRP denotes They
T14186 80-83 VBP denotes are
T14187 83-85 , denotes ,
T14188 85-92 RB denotes however
T14189 92-94 , denotes ,
T14190 94-102 RB denotes severely
T14191 103-112 JJ denotes defective
T14192 113-115 IN denotes in
T14193 116-121 PRP$ denotes their
T14194 122-129 NN denotes ability
T14195 130-132 TO denotes to
T14196 133-144 VB denotes concentrate
T14197 145-150 NN denotes urine
T14198 150-152 , denotes ,
T14199 152-159 VBG denotes leading
T14200 160-162 IN denotes to
T14201 163-172 VBN denotes increased
T14202 179-185 NN denotes output
T14203 173-178 NN denotes urine
T14204 186-189 CC denotes and
T14205 190-195 NN denotes water
T14206 196-202 NN denotes intake
T14207 202-204 , denotes ,
T14208 204-208 RB denotes thus
T14209 209-215 VBG denotes making
T14210 216-220 PRP denotes them
T14211 237-242 NN denotes model
T14212 221-224 DT denotes the
T14213 225-230 JJ denotes first
T14214 231-236 NN denotes mouse
T14215 243-245 IN denotes of
T14216 246-249 NN denotes NDI
T14217 250-252 TO denotes to
T14218 253-260 VB denotes survive
T14219 261-263 IN denotes to
T14220 264-272 NN denotes maturity
T14221 272-273 . denotes .
T14222 273-329 sentence denotes In humans, NDI is caused by mutations in Avpr2 or Aqp2.
T14223 274-276 IN denotes In
T14224 292-298 VBN denotes caused
T14225 277-283 NNS denotes humans
T14226 283-285 , denotes ,
T14227 285-288 NN denotes NDI
T14228 289-291 VBZ denotes is
T14229 299-301 IN denotes by
T14230 302-311 NNS denotes mutations
T14231 312-314 IN denotes in
T14232 315-320 NN denotes Avpr2
T14233 321-323 CC denotes or
T14234 324-328 NN denotes Aqp2
T14235 328-329 . denotes .
T14236 329-524 sentence denotes Knockout of the X-linked Avpr2 gene in mice [15] gave an NDI-like phenotype in male, hemizygous neonates, but the phenotype could not be assessed in adults as the mice died within 1 wk of birth.
T14237 330-338 NN denotes Knockout
T14238 379-383 VBD denotes gave
T14239 339-341 IN denotes of
T14240 342-345 DT denotes the
T14241 361-365 NN denotes gene
T14242 346-347 NN denotes X
T14243 348-354 VBN denotes linked
T14244 347-348 HYPH denotes -
T14245 355-360 NN denotes Avpr2
T14246 366-368 IN denotes in
T14247 369-373 NNS denotes mice
T14248 374-375 -LRB- denotes [
T14249 375-377 CD denotes 15
T14250 377-378 -RRB- denotes ]
T14251 384-386 DT denotes an
T14252 396-405 NN denotes phenotype
T14253 387-390 NN denotes NDI
T14254 391-395 JJ denotes like
T14255 390-391 HYPH denotes -
T14256 406-408 IN denotes in
T14257 409-413 JJ denotes male
T14258 426-434 NNS denotes neonates
T14259 413-415 , denotes ,
T14260 415-425 JJ denotes hemizygous
T14261 434-436 , denotes ,
T14262 436-439 CC denotes but
T14263 440-443 DT denotes the
T14264 444-453 NN denotes phenotype
T14265 467-475 VBN denotes assessed
T14266 454-459 MD denotes could
T14267 460-463 RB denotes not
T14268 464-466 VB denotes be
T14269 476-478 IN denotes in
T14270 479-485 NNS denotes adults
T14271 486-488 IN denotes as
T14272 498-502 VBD denotes died
T14273 489-492 DT denotes the
T14274 493-497 NNS denotes mice
T14275 503-509 IN denotes within
T14276 510-511 CD denotes 1
T14277 512-514 NN denotes wk
T14278 515-517 IN denotes of
T14279 518-523 NN denotes birth
T14280 523-524 . denotes .
T14281 524-659 sentence denotes The adult heterozygous females showed a mild tendency toward increased urinary output and water intake and decreased urine osmolality.
T14282 525-528 DT denotes The
T14283 548-555 NNS denotes females
T14284 529-534 JJ denotes adult
T14285 535-547 JJ denotes heterozygous
T14286 556-562 VBD denotes showed
T14287 563-564 DT denotes a
T14288 570-578 NN denotes tendency
T14289 565-569 JJ denotes mild
T14290 579-585 IN denotes toward
T14291 586-595 VBN denotes increased
T14292 604-610 NN denotes output
T14293 596-603 JJ denotes urinary
T14294 611-614 CC denotes and
T14295 615-620 NN denotes water
T14296 621-627 NN denotes intake
T14297 628-631 CC denotes and
T14298 632-641 VBN denotes decreased
T14299 648-658 NN denotes osmolality
T14300 642-647 NN denotes urine
T14301 658-659 . denotes .
T14302 659-714 sentence denotes Knockout of the mouse Aqp2 gene has not been reported.
T14303 660-668 NN denotes Knockout
T14304 705-713 VBN denotes reported
T14305 669-671 IN denotes of
T14306 672-675 DT denotes the
T14307 687-691 NN denotes gene
T14308 676-681 NN denotes mouse
T14309 682-686 NN denotes Aqp2
T14310 692-695 VBZ denotes has
T14311 696-699 RB denotes not
T14312 700-704 VBN denotes been
T14313 713-714 . denotes .
T14314 714-799 sentence denotes A knock-in of a human disease-causing mutation (T126M), however, has been made [18].
T14315 715-716 DT denotes A
T14316 723-725 NN denotes in
T14317 717-722 NN denotes knock
T14318 722-723 HYPH denotes -
T14319 789-793 VBN denotes made
T14320 726-728 IN denotes of
T14321 729-730 DT denotes a
T14322 753-761 NN denotes mutation
T14323 731-736 JJ denotes human
T14324 737-744 NN denotes disease
T14325 745-752 VBG denotes causing
T14326 744-745 HYPH denotes -
T14327 762-763 -LRB- denotes (
T14328 763-768 NN denotes T126M
T14329 768-769 -RRB- denotes )
T14330 769-771 , denotes ,
T14331 771-778 RB denotes however
T14332 778-780 , denotes ,
T14333 780-783 VBZ denotes has
T14334 784-788 VBN denotes been
T14335 794-795 -LRB- denotes [
T14336 795-797 CD denotes 18
T14337 797-798 -RRB- denotes ]
T14338 798-799 . denotes .
T14339 799-908 sentence denotes These mice have a severe urine-concentrating defect resulting in dehydration and death within 1 wk of birth.
T14340 800-805 DT denotes These
T14341 806-810 NNS denotes mice
T14342 811-815 VBP denotes have
T14343 816-817 DT denotes a
T14344 845-851 NN denotes defect
T14345 818-824 JJ denotes severe
T14346 825-830 NN denotes urine
T14347 831-844 VBG denotes concentrating
T14348 830-831 HYPH denotes -
T14349 852-861 VBG denotes resulting
T14350 862-864 IN denotes in
T14351 865-876 NN denotes dehydration
T14352 877-880 CC denotes and
T14353 881-886 NN denotes death
T14354 887-893 IN denotes within
T14355 894-895 CD denotes 1
T14356 896-898 NN denotes wk
T14357 899-901 IN denotes of
T14358 902-907 NN denotes birth
T14359 907-908 . denotes .
T14360 908-984 sentence denotes Curiously, AQP2-T126M does localize properly in at least a subset of cells.
T14361 909-918 RB denotes Curiously
T14362 936-944 VB denotes localize
T14363 918-920 , denotes ,
T14364 920-924 NN denotes AQP2
T14365 925-930 NN denotes T126M
T14366 924-925 HYPH denotes -
T14367 931-935 VBZ denotes does
T14368 945-953 RB denotes properly
T14369 954-956 IN denotes in
T14370 957-959 RB denotes at
T14371 960-965 RBS denotes least
T14372 968-974 NN denotes subset
T14373 966-967 DT denotes a
T14374 975-977 IN denotes of
T14375 978-983 NNS denotes cells
T14376 983-984 . denotes .
T14377 984-1109 sentence denotes The grossly abnormal collecting duct morphology makes it impossible to pinpoint the molecular defect in these knock-in mice.
T14378 985-988 DT denotes The
T14379 1022-1032 NN denotes morphology
T14380 989-996 RB denotes grossly
T14381 997-1005 JJ denotes abnormal
T14382 1006-1016 VBG denotes collecting
T14383 1017-1021 NN denotes duct
T14384 1033-1038 VBZ denotes makes
T14385 1039-1041 PRP denotes it
T14386 1042-1052 JJ denotes impossible
T14387 1053-1055 TO denotes to
T14388 1056-1064 VB denotes pinpoint
T14389 1065-1068 DT denotes the
T14390 1079-1085 NN denotes defect
T14391 1069-1078 JJ denotes molecular
T14392 1086-1088 IN denotes in
T14393 1089-1094 DT denotes these
T14394 1104-1108 NNS denotes mice
T14395 1095-1100 VB denotes knock
T14396 1100-1101 HYPH denotes -
T14397 1101-1103 RP denotes in
T14398 1108-1109 . denotes .
T14399 1109-1179 sentence denotes The T126M knock-in clearly shows that Aqp2 is an essential gene [18].
T14400 1110-1113 DT denotes The
T14401 1126-1128 NN denotes in
T14402 1114-1119 NN denotes T126M
T14403 1120-1125 NN denotes knock
T14404 1125-1126 HYPH denotes -
T14405 1137-1142 VBZ denotes shows
T14406 1129-1136 RB denotes clearly
T14407 1143-1147 IN denotes that
T14408 1153-1155 VBZ denotes is
T14409 1148-1152 NN denotes Aqp2
T14410 1156-1158 DT denotes an
T14411 1169-1173 NN denotes gene
T14412 1159-1168 JJ denotes essential
T14413 1174-1175 -LRB- denotes [
T14414 1175-1177 CD denotes 18
T14415 1177-1178 -RRB- denotes ]
T14416 1178-1179 . denotes .
T14417 1179-1357 sentence denotes The fact that our mice survive shows either that AQP2-F204V possesses some residual water transporting ability or that there are AVP-independent pathways for water reabsorption.
T14418 1180-1183 DT denotes The
T14419 1184-1188 NN denotes fact
T14420 1211-1216 VBZ denotes shows
T14421 1189-1193 IN denotes that
T14422 1203-1210 VBP denotes survive
T14423 1194-1197 PRP$ denotes our
T14424 1198-1202 NNS denotes mice
T14425 1217-1223 CC denotes either
T14426 1240-1249 VBZ denotes possesses
T14427 1224-1228 IN denotes that
T14428 1229-1233 NN denotes AQP2
T14429 1234-1239 NN denotes F204V
T14430 1233-1234 HYPH denotes -
T14431 1250-1254 DT denotes some
T14432 1283-1290 NN denotes ability
T14433 1255-1263 JJ denotes residual
T14434 1264-1269 NN denotes water
T14435 1270-1282 VBG denotes transporting
T14436 1291-1293 CC denotes or
T14437 1294-1298 IN denotes that
T14438 1305-1308 VBP denotes are
T14439 1299-1304 EX denotes there
T14440 1309-1312 NN denotes AVP
T14441 1313-1324 JJ denotes independent
T14442 1312-1313 HYPH denotes -
T14443 1325-1333 NNS denotes pathways
T14444 1334-1337 IN denotes for
T14445 1338-1343 NN denotes water
T14446 1344-1356 NN denotes reabsorption
T14447 1356-1357 . denotes .
T14448 1357-1515 sentence denotes Residual activity of AQP2-F204V is likely, as mutant animals show some small response to dDAVP, although dDAVP-stimulated urine osmolality remains quite low.
T14449 1358-1366 JJ denotes Residual
T14450 1367-1375 NN denotes activity
T14451 1390-1392 VBZ denotes is
T14452 1376-1378 IN denotes of
T14453 1379-1383 NN denotes AQP2
T14454 1384-1389 NN denotes F204V
T14455 1383-1384 HYPH denotes -
T14456 1393-1399 JJ denotes likely
T14457 1399-1401 , denotes ,
T14458 1401-1403 IN denotes as
T14459 1419-1423 VBP denotes show
T14460 1404-1410 NN denotes mutant
T14461 1411-1418 NNS denotes animals
T14462 1424-1428 DT denotes some
T14463 1435-1443 NN denotes response
T14464 1429-1434 JJ denotes small
T14465 1444-1446 IN denotes to
T14466 1447-1452 NN denotes dDAVP
T14467 1452-1454 , denotes ,
T14468 1454-1462 IN denotes although
T14469 1497-1504 VBZ denotes remains
T14470 1463-1468 NN denotes dDAVP
T14471 1469-1479 VBN denotes stimulated
T14472 1468-1469 HYPH denotes -
T14473 1486-1496 NN denotes osmolality
T14474 1480-1485 NN denotes urine
T14475 1505-1510 RB denotes quite
T14476 1511-1514 JJ denotes low
T14477 1514-1515 . denotes .
T14478 1515-1679 sentence denotes Immunostaining of kidney shows that AQP2-F204V does not efficiently transport water, because it fails to localize to the apical cell surface after dDAVP treatment.
T14479 1516-1530 NN denotes Immunostaining
T14480 1541-1546 VBZ denotes shows
T14481 1531-1533 IN denotes of
T14482 1534-1540 NN denotes kidney
T14483 1547-1551 IN denotes that
T14484 1584-1593 VB denotes transport
T14485 1552-1556 NN denotes AQP2
T14486 1557-1562 NN denotes F204V
T14487 1556-1557 HYPH denotes -
T14488 1563-1567 VBZ denotes does
T14489 1568-1571 RB denotes not
T14490 1572-1583 RB denotes efficiently
T14491 1594-1599 NN denotes water
T14492 1599-1601 , denotes ,
T14493 1601-1608 IN denotes because
T14494 1612-1617 VBZ denotes fails
T14495 1609-1611 PRP denotes it
T14496 1618-1620 TO denotes to
T14497 1621-1629 VB denotes localize
T14498 1630-1632 IN denotes to
T14499 1633-1636 DT denotes the
T14500 1649-1656 NN denotes surface
T14501 1637-1643 JJ denotes apical
T14502 1644-1648 NN denotes cell
T14503 1657-1662 IN denotes after
T14504 1663-1668 NN denotes dDAVP
T14505 1669-1678 NN denotes treatment
T14506 1678-1679 . denotes .
T14507 1679-1814 sentence denotes Some residual activity of AQP2 would imply that some small, undetectable portion of the mutant protein is getting to the cell surface.
T14508 1680-1684 DT denotes Some
T14509 1694-1702 NN denotes activity
T14510 1685-1693 JJ denotes residual
T14511 1717-1722 VB denotes imply
T14512 1703-1705 IN denotes of
T14513 1706-1710 NN denotes AQP2
T14514 1711-1716 MD denotes would
T14515 1723-1727 IN denotes that
T14516 1786-1793 VBG denotes getting
T14517 1728-1732 DT denotes some
T14518 1753-1760 NN denotes portion
T14519 1733-1738 JJ denotes small
T14520 1738-1740 , denotes ,
T14521 1740-1752 JJ denotes undetectable
T14522 1761-1763 IN denotes of
T14523 1764-1767 DT denotes the
T14524 1775-1782 NN denotes protein
T14525 1768-1774 NN denotes mutant
T14526 1783-1785 VBZ denotes is
T14527 1794-1796 IN denotes to
T14528 1797-1800 DT denotes the
T14529 1806-1813 NN denotes surface
T14530 1801-1805 NN denotes cell
T14531 1813-1814 . denotes .
T14532 1814-1977 sentence denotes The surface biotinylation experiment (Figure 5D) suggests that no mutant protein gets to the surface, but this does not necessarily reflect the situation in vivo.
T14533 1815-1818 DT denotes The
T14534 1841-1851 NN denotes experiment
T14535 1819-1826 NN denotes surface
T14536 1827-1840 NN denotes biotinylation
T14537 1864-1872 VBZ denotes suggests
T14538 1852-1853 -LRB- denotes (
T14539 1853-1859 NN denotes Figure
T14540 1860-1862 CD denotes 5D
T14541 1862-1863 -RRB- denotes )
T14542 1873-1877 IN denotes that
T14543 1896-1900 VBZ denotes gets
T14544 1878-1880 DT denotes no
T14545 1888-1895 NN denotes protein
T14546 1881-1887 NN denotes mutant
T14547 1901-1903 IN denotes to
T14548 1904-1907 DT denotes the
T14549 1908-1915 NN denotes surface
T14550 1915-1917 , denotes ,
T14551 1917-1920 CC denotes but
T14552 1921-1925 DT denotes this
T14553 1947-1954 VB denotes reflect
T14554 1926-1930 VBZ denotes does
T14555 1931-1934 RB denotes not
T14556 1935-1946 RB denotes necessarily
T14557 1955-1958 DT denotes the
T14558 1959-1968 NN denotes situation
T14559 1969-1971 FW denotes in
T14560 1972-1976 FW denotes vivo
T14561 1976-1977 . denotes .
T14562 1977-2169 sentence denotes While this small fraction of protein may not be detectable by immunofluorescence, Western blotting shows that some mutant protein does progress beyond the ER (35–45 kDa species in Figure 3A).
T14563 1978-1983 IN denotes While
T14564 2023-2025 VB denotes be
T14565 1984-1988 DT denotes this
T14566 1995-2003 NN denotes fraction
T14567 1989-1994 JJ denotes small
T14568 2004-2006 IN denotes of
T14569 2007-2014 NN denotes protein
T14570 2015-2018 MD denotes may
T14571 2019-2022 RB denotes not
T14572 2077-2082 VBZ denotes shows
T14573 2026-2036 JJ denotes detectable
T14574 2037-2039 IN denotes by
T14575 2040-2058 NN denotes immunofluorescence
T14576 2058-2060 , denotes ,
T14577 2060-2067 NNP denotes Western
T14578 2068-2076 NN denotes blotting
T14579 2083-2087 IN denotes that
T14580 2113-2121 VB denotes progress
T14581 2088-2092 DT denotes some
T14582 2100-2107 NN denotes protein
T14583 2093-2099 NN denotes mutant
T14584 2108-2112 VBZ denotes does
T14585 2122-2128 IN denotes beyond
T14586 2129-2132 DT denotes the
T14587 2133-2135 NN denotes ER
T14588 2136-2137 -LRB- denotes (
T14589 2147-2154 NNS denotes species
T14590 2137-2139 CD denotes 35
T14591 2140-2142 CD denotes 45
T14592 2139-2140 SYM denotes –
T14593 2143-2146 NN denotes kDa
T14594 2155-2157 IN denotes in
T14595 2158-2164 NN denotes Figure
T14596 2165-2167 CD denotes 3A
T14597 2167-2168 -RRB- denotes )
T14598 2168-2169 . denotes .
T14599 2169-2358 sentence denotes Compared to wild-type, mutant protein is enriched in the high-mannose, core-glycosylated form (31 kDa) and deficient in nonglycosylated (29 kDa) and complex glycosylated (35–45 kDa) forms.
T14600 2170-2178 VBN denotes Compared
T14601 2208-2210 VBZ denotes is
T14602 2179-2181 IN denotes to
T14603 2182-2186 JJ denotes wild
T14604 2187-2191 NN denotes type
T14605 2186-2187 HYPH denotes -
T14606 2191-2193 , denotes ,
T14607 2193-2199 NN denotes mutant
T14608 2200-2207 NN denotes protein
T14609 2211-2219 VBN denotes enriched
T14610 2220-2222 IN denotes in
T14611 2223-2226 DT denotes the
T14612 2259-2263 NN denotes form
T14613 2227-2231 JJ denotes high
T14614 2232-2239 NN denotes mannose
T14615 2231-2232 HYPH denotes -
T14616 2239-2241 , denotes ,
T14617 2241-2245 NN denotes core
T14618 2246-2258 VBN denotes glycosylated
T14619 2245-2246 HYPH denotes -
T14620 2264-2265 -LRB- denotes (
T14621 2268-2271 NN denotes kDa
T14622 2265-2267 CD denotes 31
T14623 2271-2272 -RRB- denotes )
T14624 2273-2276 CC denotes and
T14625 2277-2286 JJ denotes deficient
T14626 2287-2289 IN denotes in
T14627 2290-2305 JJ denotes nonglycosylated
T14628 2352-2357 NNS denotes forms
T14629 2306-2307 -LRB- denotes (
T14630 2310-2313 NN denotes kDa
T14631 2307-2309 CD denotes 29
T14632 2313-2314 -RRB- denotes )
T14633 2315-2318 CC denotes and
T14634 2319-2326 JJ denotes complex
T14635 2327-2339 JJ denotes glycosylated
T14636 2340-2341 -LRB- denotes (
T14637 2347-2350 NN denotes kDa
T14638 2341-2343 CD denotes 35
T14639 2344-2346 CD denotes 45
T14640 2343-2344 SYM denotes –
T14641 2350-2351 -RRB- denotes )
T14642 2357-2358 . denotes .
T14643 2358-2537 sentence denotes The presence of a reduced but detectable amount of protein in the 35–45 kDa range indicates that mutant protein is transported out of the ER, but with greatly reduced efficiency.
T14644 2359-2362 DT denotes The
T14645 2363-2371 NN denotes presence
T14646 2441-2450 VBZ denotes indicates
T14647 2372-2374 IN denotes of
T14648 2375-2376 DT denotes a
T14649 2400-2406 NN denotes amount
T14650 2377-2384 VBN denotes reduced
T14651 2385-2388 CC denotes but
T14652 2389-2399 JJ denotes detectable
T14653 2407-2409 IN denotes of
T14654 2410-2417 NN denotes protein
T14655 2418-2420 IN denotes in
T14656 2421-2424 DT denotes the
T14657 2435-2440 NN denotes range
T14658 2425-2427 CD denotes 35
T14659 2428-2430 CD denotes 45
T14660 2427-2428 SYM denotes –
T14661 2431-2434 NN denotes kDa
T14662 2451-2455 IN denotes that
T14663 2474-2485 VBN denotes transported
T14664 2456-2462 NN denotes mutant
T14665 2463-2470 NN denotes protein
T14666 2471-2473 VBZ denotes is
T14667 2486-2489 IN denotes out
T14668 2490-2492 IN denotes of
T14669 2493-2496 DT denotes the
T14670 2497-2499 NN denotes ER
T14671 2499-2501 , denotes ,
T14672 2501-2504 CC denotes but
T14673 2505-2509 IN denotes with
T14674 2510-2517 RB denotes greatly
T14675 2518-2525 VBN denotes reduced
T14676 2526-2536 NN denotes efficiency
T14677 2536-2537 . denotes .
T14678 2537-2722 sentence denotes Colocalization of AQP2-F204V with the ER protein calnexin in transfected MDCK cells shows that, while most of the mutant protein is trapped in the ER, some does progress beyond the ER.
T14679 2538-2552 NN denotes Colocalization
T14680 2622-2627 VBZ denotes shows
T14681 2553-2555 IN denotes of
T14682 2556-2560 NN denotes AQP2
T14683 2561-2566 NN denotes F204V
T14684 2560-2561 HYPH denotes -
T14685 2567-2571 IN denotes with
T14686 2572-2575 DT denotes the
T14687 2579-2586 NN denotes protein
T14688 2576-2578 NN denotes ER
T14689 2587-2595 NN denotes calnexin
T14690 2596-2598 IN denotes in
T14691 2599-2610 VBN denotes transfected
T14692 2616-2621 NNS denotes cells
T14693 2611-2615 NN denotes MDCK
T14694 2628-2632 IN denotes that
T14695 2699-2707 VB denotes progress
T14696 2632-2634 , denotes ,
T14697 2634-2639 IN denotes while
T14698 2670-2677 VBN denotes trapped
T14699 2640-2644 JJS denotes most
T14700 2645-2647 IN denotes of
T14701 2648-2651 DT denotes the
T14702 2659-2666 NN denotes protein
T14703 2652-2658 NN denotes mutant
T14704 2667-2669 VBZ denotes is
T14705 2678-2680 IN denotes in
T14706 2681-2684 DT denotes the
T14707 2685-2687 NN denotes ER
T14708 2687-2689 , denotes ,
T14709 2689-2693 DT denotes some
T14710 2694-2698 VBZ denotes does
T14711 2708-2714 IN denotes beyond
T14712 2715-2718 DT denotes the
T14713 2719-2721 NN denotes ER
T14714 2721-2722 . denotes .
T14715 2722-3040 sentence denotes Diminished response to dDAVP, diminished abundance of mature glycosylated protein in mutant animals, and the transport of a fraction of mutant protein beyond the ER in MDCK cells are all consistent with the notion that AQP2-F204V misfolding is limited and that it may retain some residual water transporting activity.
T14716 2723-2733 VBN denotes Diminished
T14717 2734-2742 NN denotes response
T14718 2902-2905 VBP denotes are
T14719 2743-2745 IN denotes to
T14720 2746-2751 NN denotes dDAVP
T14721 2751-2753 , denotes ,
T14722 2753-2763 VBN denotes diminished
T14723 2764-2773 NN denotes abundance
T14724 2774-2776 IN denotes of
T14725 2777-2783 JJ denotes mature
T14726 2797-2804 NN denotes protein
T14727 2784-2796 VBN denotes glycosylated
T14728 2805-2807 IN denotes in
T14729 2808-2814 NN denotes mutant
T14730 2815-2822 NNS denotes animals
T14731 2822-2824 , denotes ,
T14732 2824-2827 CC denotes and
T14733 2828-2831 DT denotes the
T14734 2832-2841 NN denotes transport
T14735 2842-2844 IN denotes of
T14736 2845-2846 DT denotes a
T14737 2847-2855 NN denotes fraction
T14738 2856-2858 IN denotes of
T14739 2859-2865 NN denotes mutant
T14740 2866-2873 NN denotes protein
T14741 2874-2880 IN denotes beyond
T14742 2881-2884 DT denotes the
T14743 2885-2887 NN denotes ER
T14744 2888-2890 IN denotes in
T14745 2891-2895 NN denotes MDCK
T14746 2896-2901 NNS denotes cells
T14747 2906-2909 RB denotes all
T14748 2910-2920 JJ denotes consistent
T14749 2921-2925 IN denotes with
T14750 2926-2929 DT denotes the
T14751 2930-2936 NN denotes notion
T14752 2937-2941 IN denotes that
T14753 2964-2966 VBZ denotes is
T14754 2942-2946 NN denotes AQP2
T14755 2947-2952 NN denotes F204V
T14756 2946-2947 HYPH denotes -
T14757 2953-2963 NN denotes misfolding
T14758 2967-2974 JJ denotes limited
T14759 2975-2978 CC denotes and
T14760 2979-2983 IN denotes that
T14761 2991-2997 VB denotes retain
T14762 2984-2986 PRP denotes it
T14763 2987-2990 MD denotes may
T14764 2998-3002 DT denotes some
T14765 3031-3039 NN denotes activity
T14766 3003-3011 JJ denotes residual
T14767 3012-3017 NN denotes water
T14768 3018-3030 VBG denotes transporting
T14769 3039-3040 . denotes .
T14770 3040-3135 sentence denotes Evidently this residual activity is sufficient for the viability and growth of mutant animals.
T14771 3041-3050 RB denotes Evidently
T14772 3074-3076 VBZ denotes is
T14773 3051-3055 DT denotes this
T14774 3065-3073 NN denotes activity
T14775 3056-3064 JJ denotes residual
T14776 3077-3087 JJ denotes sufficient
T14777 3088-3091 IN denotes for
T14778 3092-3095 DT denotes the
T14779 3096-3105 NN denotes viability
T14780 3106-3109 CC denotes and
T14781 3110-3116 NN denotes growth
T14782 3117-3119 IN denotes of
T14783 3120-3126 NN denotes mutant
T14784 3127-3134 NNS denotes animals
T14785 3134-3135 . denotes .
T14786 3135-3257 sentence denotes Reduced efficiency in exiting the ER may explain why AQP2-F204V is enriched in the 31 kDa high-mannose glycosylated form.
T14787 3136-3143 VBN denotes Reduced
T14788 3144-3154 NN denotes efficiency
T14789 3177-3184 VB denotes explain
T14790 3155-3157 IN denotes in
T14791 3158-3165 VBG denotes exiting
T14792 3166-3169 DT denotes the
T14793 3170-3172 NN denotes ER
T14794 3173-3176 MD denotes may
T14795 3185-3188 WRB denotes why
T14796 3203-3211 VBN denotes enriched
T14797 3189-3193 NN denotes AQP2
T14798 3194-3199 NN denotes F204V
T14799 3193-3194 HYPH denotes -
T14800 3200-3202 VBZ denotes is
T14801 3212-3214 IN denotes in
T14802 3215-3218 DT denotes the
T14803 3252-3256 NN denotes form
T14804 3219-3221 CD denotes 31
T14805 3222-3225 NN denotes kDa
T14806 3226-3230 JJ denotes high
T14807 3231-3238 NN denotes mannose
T14808 3230-3231 HYPH denotes -
T14809 3239-3251 VBN denotes glycosylated
T14810 3256-3257 . denotes .
T14811 3257-3380 sentence denotes The high-mannose core oligosaccharide is added in the ER and is later modified and elaborated in the Golgi apparatus [26].
T14812 3258-3261 DT denotes The
T14813 3280-3295 NN denotes oligosaccharide
T14814 3262-3266 JJ denotes high
T14815 3267-3274 NN denotes mannose
T14816 3266-3267 HYPH denotes -
T14817 3275-3279 NN denotes core
T14818 3299-3304 VBN denotes added
T14819 3296-3298 VBZ denotes is
T14820 3305-3307 IN denotes in
T14821 3308-3311 DT denotes the
T14822 3312-3314 NN denotes ER
T14823 3315-3318 CC denotes and
T14824 3319-3321 VBZ denotes is
T14825 3328-3336 VBN denotes modified
T14826 3322-3327 RB denotes later
T14827 3337-3340 CC denotes and
T14828 3341-3351 VBN denotes elaborated
T14829 3352-3354 IN denotes in
T14830 3355-3358 DT denotes the
T14831 3365-3374 NN denotes apparatus
T14832 3359-3364 NN denotes Golgi
T14833 3375-3376 -LRB- denotes [
T14834 3376-3378 CD denotes 26
T14835 3378-3379 -RRB- denotes ]
T14836 3379-3380 . denotes .
T14837 3380-3541 sentence denotes The increase in the high-mannose glycosylated form of AQP2-F204V may simply reflect its prolonged presence in the ER and exposure to oligosaccharyl transferase.
T14838 3381-3384 DT denotes The
T14839 3385-3393 NN denotes increase
T14840 3457-3464 VB denotes reflect
T14841 3394-3396 IN denotes in
T14842 3397-3400 DT denotes the
T14843 3427-3431 NN denotes form
T14844 3401-3405 JJ denotes high
T14845 3406-3413 NN denotes mannose
T14846 3405-3406 HYPH denotes -
T14847 3414-3426 VBN denotes glycosylated
T14848 3432-3434 IN denotes of
T14849 3435-3439 NN denotes AQP2
T14850 3440-3445 NN denotes F204V
T14851 3439-3440 HYPH denotes -
T14852 3446-3449 MD denotes may
T14853 3450-3456 RB denotes simply
T14854 3465-3468 PRP$ denotes its
T14855 3479-3487 NN denotes presence
T14856 3469-3478 JJ denotes prolonged
T14857 3488-3490 IN denotes in
T14858 3491-3494 DT denotes the
T14859 3495-3497 NN denotes ER
T14860 3498-3501 CC denotes and
T14861 3502-3510 NN denotes exposure
T14862 3511-3513 IN denotes to
T14863 3514-3528 NN denotes oligosaccharyl
T14864 3529-3540 NN denotes transferase
T14865 3540-3541 . denotes .
T14866 3541-3711 sentence denotes While improper localization of AQP2 explains the phenotype of homozygous mutant mice, the complete lack of a phenotype in heterozygous mice is more difficult to explain.
T14867 3542-3547 IN denotes While
T14868 3578-3586 VBZ denotes explains
T14869 3548-3556 JJ denotes improper
T14870 3557-3569 NN denotes localization
T14871 3570-3572 IN denotes of
T14872 3573-3577 NN denotes AQP2
T14873 3682-3684 VBZ denotes is
T14874 3587-3590 DT denotes the
T14875 3591-3600 NN denotes phenotype
T14876 3601-3603 IN denotes of
T14877 3604-3614 JJ denotes homozygous
T14878 3622-3626 NNS denotes mice
T14879 3615-3621 NN denotes mutant
T14880 3626-3628 , denotes ,
T14881 3628-3631 DT denotes the
T14882 3641-3645 NN denotes lack
T14883 3632-3640 JJ denotes complete
T14884 3646-3648 IN denotes of
T14885 3649-3650 DT denotes a
T14886 3651-3660 NN denotes phenotype
T14887 3661-3663 IN denotes in
T14888 3664-3676 JJ denotes heterozygous
T14889 3677-3681 NNS denotes mice
T14890 3685-3689 RBR denotes more
T14891 3690-3699 JJ denotes difficult
T14892 3700-3702 TO denotes to
T14893 3703-3710 VB denotes explain
T14894 3710-3711 . denotes .
T14895 3711-3868 sentence denotes Physiologically, heterozygous mice have no symptoms (see Figure 1C), and they are indistinguishable from wild type on immunostaining of kidneys (Figure 5A).
T14896 3712-3727 RB denotes Physiologically
T14897 3747-3751 VBP denotes have
T14898 3727-3729 , denotes ,
T14899 3729-3741 JJ denotes heterozygous
T14900 3742-3746 NNS denotes mice
T14901 3752-3754 DT denotes no
T14902 3755-3763 NNS denotes symptoms
T14903 3764-3765 -LRB- denotes (
T14904 3765-3768 VB denotes see
T14905 3769-3775 NN denotes Figure
T14906 3776-3778 CD denotes 1C
T14907 3778-3779 -RRB- denotes )
T14908 3779-3781 , denotes ,
T14909 3781-3784 CC denotes and
T14910 3785-3789 PRP denotes they
T14911 3790-3793 VBP denotes are
T14912 3794-3811 JJ denotes indistinguishable
T14913 3812-3816 IN denotes from
T14914 3817-3821 JJ denotes wild
T14915 3822-3826 NN denotes type
T14916 3827-3829 IN denotes on
T14917 3830-3844 NN denotes immunostaining
T14918 3845-3847 IN denotes of
T14919 3848-3855 NNS denotes kidneys
T14920 3856-3857 -LRB- denotes (
T14921 3857-3863 NN denotes Figure
T14922 3864-3866 CD denotes 5A
T14923 3866-3867 -RRB- denotes )
T14924 3867-3868 . denotes .
T14925 3868-4030 sentence denotes The presence of 50% of the normal amount of wild-type protein may explain the lack of symptoms, but it cannot explain the lack of any ER-retained mutant protein.
T14926 3869-3872 DT denotes The
T14927 3873-3881 NN denotes presence
T14928 3935-3942 VB denotes explain
T14929 3882-3884 IN denotes of
T14930 3885-3887 CD denotes 50
T14931 3887-3888 NN denotes %
T14932 3889-3891 IN denotes of
T14933 3892-3895 DT denotes the
T14934 3903-3909 NN denotes amount
T14935 3896-3902 JJ denotes normal
T14936 3910-3912 IN denotes of
T14937 3913-3917 JJ denotes wild
T14938 3918-3922 NN denotes type
T14939 3917-3918 HYPH denotes -
T14940 3923-3930 NN denotes protein
T14941 3931-3934 MD denotes may
T14942 3943-3946 DT denotes the
T14943 3947-3951 NN denotes lack
T14944 3952-3954 IN denotes of
T14945 3955-3963 NNS denotes symptoms
T14946 3963-3965 , denotes ,
T14947 3965-3968 CC denotes but
T14948 3969-3971 PRP denotes it
T14949 3979-3986 VB denotes explain
T14950 3972-3975 MD denotes can
T14951 3975-3978 RB denotes not
T14952 3987-3990 DT denotes the
T14953 3991-3995 NN denotes lack
T14954 3996-3998 IN denotes of
T14955 3999-4002 DT denotes any
T14956 4022-4029 NN denotes protein
T14957 4003-4005 NN denotes ER
T14958 4006-4014 VBN denotes retained
T14959 4005-4006 HYPH denotes -
T14960 4015-4021 NN denotes mutant
T14961 4029-4030 . denotes .
T14962 4030-4155 sentence denotes Rather, the phenotype of the Aqp2F204V/+ animals suggests that the mutant protein is being rescued by the wild-type protein.
T14963 4031-4037 RB denotes Rather
T14964 4080-4088 VBZ denotes suggests
T14965 4037-4039 , denotes ,
T14966 4039-4042 DT denotes the
T14967 4043-4052 NN denotes phenotype
T14968 4053-4055 IN denotes of
T14969 4056-4059 DT denotes the
T14970 4072-4079 NNS denotes animals
T14971 4060-4069 NN denotes Aqp2F204V
T14972 4069-4070 HYPH denotes /
T14973 4070-4071 SYM denotes +
T14974 4089-4093 IN denotes that
T14975 4122-4129 VBN denotes rescued
T14976 4094-4097 DT denotes the
T14977 4105-4112 NN denotes protein
T14978 4098-4104 NN denotes mutant
T14979 4113-4115 VBZ denotes is
T14980 4116-4121 VBG denotes being
T14981 4130-4132 IN denotes by
T14982 4133-4136 DT denotes the
T14983 4147-4154 NN denotes protein
T14984 4137-4141 JJ denotes wild
T14985 4142-4146 NN denotes type
T14986 4141-4142 HYPH denotes -
T14987 4154-4155 . denotes .
T14988 4155-4385 sentence denotes Indeed, de Mattia et al. (26) have demonstrated that one recessive allele of Aqp2, P262L, does not properly translocate when expressed by itself in MDCK cells, but that in the presence of wild-type protein, it localizes normally.
T14989 4156-4162 RB denotes Indeed
T14990 4191-4203 VBN denotes demonstrated
T14991 4162-4164 , denotes ,
T14992 4164-4166 NNP denotes de
T14993 4167-4173 NNP denotes Mattia
T14994 4174-4176 FW denotes et
T14995 4177-4180 FW denotes al.
T14996 4181-4182 -LRB- denotes (
T14997 4182-4184 CD denotes 26
T14998 4184-4185 -RRB- denotes )
T14999 4186-4190 VBP denotes have
T15000 4204-4208 IN denotes that
T15001 4264-4275 VB denotes translocate
T15002 4209-4212 CD denotes one
T15003 4223-4229 NN denotes allele
T15004 4213-4222 JJ denotes recessive
T15005 4230-4232 IN denotes of
T15006 4233-4237 NN denotes Aqp2
T15007 4237-4239 , denotes ,
T15008 4239-4244 NN denotes P262L
T15009 4244-4246 , denotes ,
T15010 4246-4250 VBZ denotes does
T15011 4251-4254 RB denotes not
T15012 4255-4263 RB denotes properly
T15013 4276-4280 WRB denotes when
T15014 4281-4290 VBN denotes expressed
T15015 4291-4293 IN denotes by
T15016 4294-4300 PRP denotes itself
T15017 4301-4303 IN denotes in
T15018 4304-4308 NN denotes MDCK
T15019 4309-4314 NNS denotes cells
T15020 4314-4316 , denotes ,
T15021 4316-4319 CC denotes but
T15022 4320-4324 IN denotes that
T15023 4366-4375 VBZ denotes localizes
T15024 4325-4327 IN denotes in
T15025 4328-4331 DT denotes the
T15026 4332-4340 NN denotes presence
T15027 4341-4343 IN denotes of
T15028 4344-4348 JJ denotes wild
T15029 4349-4353 NN denotes type
T15030 4348-4349 HYPH denotes -
T15031 4354-4361 NN denotes protein
T15032 4361-4363 , denotes ,
T15033 4363-4365 PRP denotes it
T15034 4376-4384 RB denotes normally
T15035 4384-4385 . denotes .
T15036 4385-4450 sentence denotes The same mechanism seems to apply in vivo with Aqp2F204V/+ mice.
T15037 4386-4389 DT denotes The
T15038 4395-4404 NN denotes mechanism
T15039 4390-4394 JJ denotes same
T15040 4405-4410 VBZ denotes seems
T15041 4411-4413 TO denotes to
T15042 4414-4419 VB denotes apply
T15043 4420-4422 FW denotes in
T15044 4423-4427 FW denotes vivo
T15045 4428-4432 IN denotes with
T15046 4433-4442 NN denotes Aqp2F204V
T15047 4445-4449 NNS denotes mice
T15048 4442-4443 HYPH denotes /
T15049 4443-4444 SYM denotes +
T15050 4449-4450 . denotes .
T15051 4450-4649 sentence denotes In support of this, AQP2-F204V can interact with wild-type AQP2 (Figure 5B), and when coexpressed with wild-type protein, AQP2-F204V can reach the cell surface (Figure 5C bottom right panel and 5D).
T15052 4451-4453 IN denotes In
T15053 4486-4494 VB denotes interact
T15054 4454-4461 NN denotes support
T15055 4462-4464 IN denotes of
T15056 4465-4469 DT denotes this
T15057 4469-4471 , denotes ,
T15058 4471-4475 NN denotes AQP2
T15059 4476-4481 NN denotes F204V
T15060 4475-4476 HYPH denotes -
T15061 4482-4485 MD denotes can
T15062 4495-4499 IN denotes with
T15063 4500-4504 JJ denotes wild
T15064 4505-4509 NN denotes type
T15065 4504-4505 HYPH denotes -
T15066 4510-4514 NN denotes AQP2
T15067 4515-4516 -LRB- denotes (
T15068 4516-4522 NN denotes Figure
T15069 4523-4525 CD denotes 5B
T15070 4525-4526 -RRB- denotes )
T15071 4526-4528 , denotes ,
T15072 4528-4531 CC denotes and
T15073 4532-4536 WRB denotes when
T15074 4537-4548 VBN denotes coexpressed
T15075 4588-4593 VB denotes reach
T15076 4549-4553 IN denotes with
T15077 4554-4558 JJ denotes wild
T15078 4559-4563 NN denotes type
T15079 4558-4559 HYPH denotes -
T15080 4564-4571 NN denotes protein
T15081 4571-4573 , denotes ,
T15082 4573-4577 NN denotes AQP2
T15083 4578-4583 NN denotes F204V
T15084 4577-4578 HYPH denotes -
T15085 4584-4587 MD denotes can
T15086 4594-4597 DT denotes the
T15087 4603-4610 NN denotes surface
T15088 4598-4602 NN denotes cell
T15089 4611-4612 -LRB- denotes (
T15090 4635-4640 NN denotes panel
T15091 4612-4618 NN denotes Figure
T15092 4619-4621 CD denotes 5C
T15093 4622-4628 NN denotes bottom
T15094 4629-4634 JJ denotes right
T15095 4641-4644 CC denotes and
T15096 4645-4647 CD denotes 5D
T15097 4647-4648 -RRB- denotes )
T15098 4648-4649 . denotes .
T15099 4649-4951 sentence denotes Although it has been demonstrated that a recessive allele (encoding AQP2-R187C) of NDI fails to interact with wild-type AQP2 [6], here we show that AQP2-F204V does interact with the wild-type protein, presumably as part of heterotetramers, and represents a rescuable allele, both in vitro and in vivo.
T15100 4650-4658 IN denotes Although
T15101 4671-4683 VBN denotes demonstrated
T15102 4659-4661 PRP denotes it
T15103 4662-4665 VBZ denotes has
T15104 4666-4670 VBN denotes been
T15105 4788-4792 VBP denotes show
T15106 4684-4688 IN denotes that
T15107 4737-4742 VBZ denotes fails
T15108 4689-4690 DT denotes a
T15109 4701-4707 NN denotes allele
T15110 4691-4700 JJ denotes recessive
T15111 4708-4709 -LRB- denotes (
T15112 4709-4717 VBG denotes encoding
T15113 4718-4722 NN denotes AQP2
T15114 4723-4728 NN denotes R187C
T15115 4722-4723 HYPH denotes -
T15116 4728-4729 -RRB- denotes )
T15117 4730-4732 IN denotes of
T15118 4733-4736 NN denotes NDI
T15119 4743-4745 TO denotes to
T15120 4746-4754 VB denotes interact
T15121 4755-4759 IN denotes with
T15122 4760-4764 JJ denotes wild
T15123 4765-4769 NN denotes type
T15124 4764-4765 HYPH denotes -
T15125 4770-4774 NN denotes AQP2
T15126 4775-4776 -LRB- denotes [
T15127 4776-4777 CD denotes 6
T15128 4777-4778 -RRB- denotes ]
T15129 4778-4780 , denotes ,
T15130 4780-4784 RB denotes here
T15131 4785-4787 PRP denotes we
T15132 4793-4797 IN denotes that
T15133 4814-4822 VB denotes interact
T15134 4798-4802 NN denotes AQP2
T15135 4803-4808 NN denotes F204V
T15136 4802-4803 HYPH denotes -
T15137 4809-4813 VBZ denotes does
T15138 4823-4827 IN denotes with
T15139 4828-4831 DT denotes the
T15140 4842-4849 NN denotes protein
T15141 4832-4836 JJ denotes wild
T15142 4837-4841 NN denotes type
T15143 4836-4837 HYPH denotes -
T15144 4849-4851 , denotes ,
T15145 4851-4861 RB denotes presumably
T15146 4862-4864 IN denotes as
T15147 4865-4869 NN denotes part
T15148 4870-4872 IN denotes of
T15149 4873-4888 NNS denotes heterotetramers
T15150 4888-4890 , denotes ,
T15151 4890-4893 CC denotes and
T15152 4894-4904 VBZ denotes represents
T15153 4905-4906 DT denotes a
T15154 4917-4923 NN denotes allele
T15155 4907-4916 JJ denotes rescuable
T15156 4923-4925 , denotes ,
T15157 4925-4929 CC denotes both
T15158 4933-4938 FW denotes vitro
T15159 4930-4932 FW denotes in
T15160 4939-4942 CC denotes and
T15161 4943-4945 FW denotes in
T15162 4946-4950 FW denotes vivo
T15163 4950-4951 . denotes .
T15164 4951-5185 sentence denotes Immunostaining the kidneys of homozygous Aqp2F204V/F204V mice shows that the mutant-expressing collecting duct cells can not mediate water reabsorption, because it fails to insert into the apical plasma membrane in response to dDAVP.
T15165 4952-4966 VBG denotes Immunostaining
T15166 5014-5019 VBZ denotes shows
T15167 4967-4970 DT denotes the
T15168 4971-4978 NNS denotes kidneys
T15169 4979-4981 IN denotes of
T15170 4982-4992 JJ denotes homozygous
T15171 5009-5013 NNS denotes mice
T15172 4993-5002 NN denotes Aqp2F204V
T15173 5003-5008 NN denotes F204V
T15174 5002-5003 HYPH denotes /
T15175 5020-5024 IN denotes that
T15176 5077-5084 VB denotes mediate
T15177 5025-5028 DT denotes the
T15178 5063-5068 NNS denotes cells
T15179 5029-5035 NN denotes mutant
T15180 5036-5046 VBG denotes expressing
T15181 5035-5036 HYPH denotes -
T15182 5047-5057 VBG denotes collecting
T15183 5058-5062 NN denotes duct
T15184 5069-5072 MD denotes can
T15185 5073-5076 RB denotes not
T15186 5085-5090 NN denotes water
T15187 5091-5103 NN denotes reabsorption
T15188 5103-5105 , denotes ,
T15189 5105-5112 IN denotes because
T15190 5116-5121 VBZ denotes fails
T15191 5113-5115 PRP denotes it
T15192 5122-5124 TO denotes to
T15193 5125-5131 VB denotes insert
T15194 5132-5136 IN denotes into
T15195 5137-5140 DT denotes the
T15196 5155-5163 NN denotes membrane
T15197 5141-5147 JJ denotes apical
T15198 5148-5154 NN denotes plasma
T15199 5164-5166 IN denotes in
T15200 5167-5175 NN denotes response
T15201 5176-5178 IN denotes to
T15202 5179-5184 NN denotes dDAVP
T15203 5184-5185 . denotes .
T15204 5185-5312 sentence denotes This is this first in vivo proof of a long-standing hypothesis that comes from in vitro studies with recessive Aqp2 mutations.
T15205 5186-5190 DT denotes This
T15206 5191-5193 VBZ denotes is
T15207 5194-5198 DT denotes this
T15208 5213-5218 NN denotes proof
T15209 5199-5204 JJ denotes first
T15210 5205-5207 FW denotes in
T15211 5208-5212 FW denotes vivo
T15212 5219-5221 IN denotes of
T15213 5222-5223 DT denotes a
T15214 5238-5248 NN denotes hypothesis
T15215 5224-5228 JJ denotes long
T15216 5229-5237 VBG denotes standing
T15217 5228-5229 HYPH denotes -
T15218 5249-5253 WDT denotes that
T15219 5254-5259 VBZ denotes comes
T15220 5260-5264 IN denotes from
T15221 5265-5267 FW denotes in
T15222 5268-5273 FW denotes vitro
T15223 5274-5281 NNS denotes studies
T15224 5282-5286 IN denotes with
T15225 5287-5296 JJ denotes recessive
T15226 5302-5311 NNS denotes mutations
T15227 5297-5301 NN denotes Aqp2
T15228 5311-5312 . denotes .
T15229 5312-5531 sentence denotes Transfection into MDCK cells of any of several Aqp2 mutations corresponding to recessive human alleles shows abnormal subcellular localization [25], [27] and failure to appropriately translocate to the plasma membrane.
T15230 5313-5325 NN denotes Transfection
T15231 5416-5421 VBZ denotes shows
T15232 5326-5330 IN denotes into
T15233 5331-5335 NN denotes MDCK
T15234 5336-5341 NNS denotes cells
T15235 5342-5344 IN denotes of
T15236 5345-5348 DT denotes any
T15237 5349-5351 IN denotes of
T15238 5352-5359 JJ denotes several
T15239 5365-5374 NNS denotes mutations
T15240 5360-5364 NN denotes Aqp2
T15241 5375-5388 VBG denotes corresponding
T15242 5389-5391 IN denotes to
T15243 5392-5401 JJ denotes recessive
T15244 5408-5415 NNS denotes alleles
T15245 5402-5407 JJ denotes human
T15246 5422-5430 JJ denotes abnormal
T15247 5443-5455 NN denotes localization
T15248 5431-5442 JJ denotes subcellular
T15249 5456-5457 -LRB- denotes [
T15250 5457-5459 CD denotes 25
T15251 5459-5460 -RRB- denotes ]
T15252 5460-5462 , denotes ,
T15253 5462-5463 -LRB- denotes [
T15254 5463-5465 CD denotes 27
T15255 5465-5466 -RRB- denotes ]
T15256 5467-5470 CC denotes and
T15257 5471-5478 NN denotes failure
T15258 5479-5481 TO denotes to
T15259 5496-5507 VB denotes translocate
T15260 5482-5495 RB denotes appropriately
T15261 5508-5510 IN denotes to
T15262 5511-5514 DT denotes the
T15263 5522-5530 NN denotes membrane
T15264 5515-5521 NN denotes plasma
T15265 5530-5531 . denotes .
T15266 5531-5678 sentence denotes Thus, misfolding, retention in the ER, and failure to translocate in response to dDAVP were proposed as the mechanism for autosomal recessive NDI.
T15267 5532-5536 RB denotes Thus
T15268 5624-5632 VBN denotes proposed
T15269 5536-5538 , denotes ,
T15270 5538-5548 NN denotes misfolding
T15271 5548-5550 , denotes ,
T15272 5550-5559 NN denotes retention
T15273 5560-5562 IN denotes in
T15274 5563-5566 DT denotes the
T15275 5567-5569 NN denotes ER
T15276 5569-5571 , denotes ,
T15277 5571-5574 CC denotes and
T15278 5575-5582 NN denotes failure
T15279 5583-5585 TO denotes to
T15280 5586-5597 VB denotes translocate
T15281 5598-5600 IN denotes in
T15282 5601-5609 NN denotes response
T15283 5610-5612 IN denotes to
T15284 5613-5618 NN denotes dDAVP
T15285 5619-5623 VBD denotes were
T15286 5633-5635 IN denotes as
T15287 5636-5639 DT denotes the
T15288 5640-5649 NN denotes mechanism
T15289 5650-5653 IN denotes for
T15290 5654-5663 JJ denotes autosomal
T15291 5674-5677 NN denotes NDI
T15292 5664-5673 JJ denotes recessive
T15293 5677-5678 . denotes .
T15294 5678-5766 sentence denotes Here we not only prove this hypothesis but also establish a useful model for human NDI.
T15295 5679-5683 RB denotes Here
T15296 5696-5701 VB denotes prove
T15297 5684-5686 PRP denotes we
T15298 5687-5690 RB denotes not
T15299 5691-5695 RB denotes only
T15300 5702-5706 DT denotes this
T15301 5707-5717 NN denotes hypothesis
T15302 5718-5721 CC denotes but
T15303 5722-5726 RB denotes also
T15304 5727-5736 VB denotes establish
T15305 5737-5738 DT denotes a
T15306 5746-5751 NN denotes model
T15307 5739-5745 JJ denotes useful
T15308 5752-5755 IN denotes for
T15309 5756-5761 JJ denotes human
T15310 5762-5765 NN denotes NDI
T15311 5765-5766 . denotes .
T15312 5766-5952 sentence denotes This mouse model of NDI based on an Aqp2 allele that can be rescued provides the opportunity to test therapies, including gene therapy, that may promote proper subcellular localization.
T15313 5767-5771 DT denotes This
T15314 5778-5783 NN denotes model
T15315 5772-5777 NN denotes mouse
T15316 5835-5843 VBZ denotes provides
T15317 5784-5786 IN denotes of
T15318 5787-5790 NN denotes NDI
T15319 5791-5796 VBN denotes based
T15320 5797-5799 IN denotes on
T15321 5800-5802 DT denotes an
T15322 5808-5814 NN denotes allele
T15323 5803-5807 NN denotes Aqp2
T15324 5815-5819 WDT denotes that
T15325 5827-5834 VBN denotes rescued
T15326 5820-5823 MD denotes can
T15327 5824-5826 VB denotes be
T15328 5844-5847 DT denotes the
T15329 5848-5859 NN denotes opportunity
T15330 5860-5862 TO denotes to
T15331 5863-5867 VB denotes test
T15332 5868-5877 NNS denotes therapies
T15333 5877-5879 , denotes ,
T15334 5879-5888 VBG denotes including
T15335 5889-5893 NN denotes gene
T15336 5894-5901 NN denotes therapy
T15337 5901-5903 , denotes ,
T15338 5903-5907 WDT denotes that
T15339 5912-5919 VB denotes promote
T15340 5908-5911 MD denotes may
T15341 5920-5926 JJ denotes proper
T15342 5939-5951 NN denotes localization
T15343 5927-5938 JJ denotes subcellular
T15344 5951-5952 . denotes .
R3378 T14172 T14173 compound Aqp2F204,F204V
R3379 T14173 T14175 compound F204V,mice
R3380 T14174 T14173 punct /,F204V
R3381 T14175 T14176 nsubj mice,are
R3382 T14177 T14176 acomp viable,are
R3383 T14178 T14176 cc and,are
R3384 T14179 T14176 conj grow,are
R3385 T14180 T14179 cc and,grow
R3386 T14181 T14179 conj reproduce,grow
R3387 T14182 T14181 advmod normally,reproduce
R3388 T14183 T14176 punct .,are
R3389 T14185 T14186 nsubj They,are
R3390 T14187 T14186 punct ", ",are
R3391 T14188 T14186 advmod however,are
R3392 T14189 T14186 punct ", ",are
R3393 T14190 T14191 advmod severely,defective
R3394 T14191 T14186 acomp defective,are
R3395 T14192 T14191 prep in,defective
R3396 T14193 T14194 poss their,ability
R3397 T14194 T14192 pobj ability,in
R3398 T14195 T14196 aux to,concentrate
R3399 T14196 T14194 acl concentrate,ability
R3400 T14197 T14196 dobj urine,concentrate
R3401 T14198 T14186 punct ", ",are
R3402 T14199 T14186 advcl leading,are
R3403 T14200 T14199 prep to,leading
R3404 T14201 T14202 amod increased,output
R3405 T14202 T14200 pobj output,to
R3406 T14203 T14202 compound urine,output
R3407 T14204 T14202 cc and,output
R3408 T14205 T14206 compound water,intake
R3409 T14206 T14202 conj intake,output
R3410 T14207 T14186 punct ", ",are
R3411 T14208 T14209 advmod thus,making
R3412 T14209 T14186 advcl making,are
R3413 T14210 T14211 nsubj them,model
R3414 T14211 T14209 ccomp model,making
R3415 T14212 T14211 det the,model
R3416 T14213 T14211 amod first,model
R3417 T14214 T14211 compound mouse,model
R3418 T14215 T14211 prep of,model
R3419 T14216 T14215 pobj NDI,of
R3420 T14217 T14218 aux to,survive
R3421 T14218 T14211 advcl survive,model
R3422 T14219 T14218 prep to,survive
R3423 T14220 T14219 pobj maturity,to
R3424 T14221 T14186 punct .,are
R3425 T14223 T14224 prep In,caused
R3426 T14225 T14223 pobj humans,In
R3427 T14226 T14224 punct ", ",caused
R3428 T14227 T14224 nsubjpass NDI,caused
R3429 T14228 T14224 auxpass is,caused
R3430 T14229 T14224 agent by,caused
R3431 T14230 T14229 pobj mutations,by
R3432 T14231 T14230 prep in,mutations
R3433 T14232 T14231 pobj Avpr2,in
R3434 T14233 T14232 cc or,Avpr2
R3435 T14234 T14232 conj Aqp2,Avpr2
R3436 T14235 T14224 punct .,caused
R3437 T14237 T14238 nsubj Knockout,gave
R3438 T14239 T14237 prep of,Knockout
R3439 T14240 T14241 det the,gene
R3440 T14241 T14239 pobj gene,of
R3441 T14242 T14243 npadvmod X,linked
R3442 T14243 T14241 amod linked,gene
R3443 T14244 T14243 punct -,linked
R3444 T14245 T14241 compound Avpr2,gene
R3445 T14246 T14237 prep in,Knockout
R3446 T14247 T14246 pobj mice,in
R3447 T14248 T14249 punct [,15
R3448 T14249 T14237 parataxis 15,Knockout
R3449 T14250 T14249 punct ],15
R3450 T14251 T14252 det an,phenotype
R3451 T14252 T14238 dobj phenotype,gave
R3452 T14253 T14254 npadvmod NDI,like
R3453 T14254 T14252 amod like,phenotype
R3454 T14255 T14254 punct -,like
R3455 T14256 T14238 prep in,gave
R3456 T14257 T14258 amod male,neonates
R3457 T14258 T14256 pobj neonates,in
R3458 T14259 T14258 punct ", ",neonates
R3459 T14260 T14258 amod hemizygous,neonates
R3460 T14261 T14238 punct ", ",gave
R3461 T14262 T14238 cc but,gave
R3462 T14263 T14264 det the,phenotype
R3463 T14264 T14265 nsubjpass phenotype,assessed
R3464 T14265 T14238 conj assessed,gave
R3465 T14266 T14265 aux could,assessed
R3466 T14267 T14265 neg not,assessed
R3467 T14268 T14265 auxpass be,assessed
R3468 T14269 T14265 prep in,assessed
R3469 T14270 T14269 pobj adults,in
R3470 T14271 T14272 mark as,died
R3471 T14272 T14265 advcl died,assessed
R3472 T14273 T14274 det the,mice
R3473 T14274 T14272 nsubj mice,died
R3474 T14275 T14272 prep within,died
R3475 T14276 T14277 nummod 1,wk
R3476 T14277 T14275 pobj wk,within
R3477 T14278 T14277 prep of,wk
R3478 T14279 T14278 pobj birth,of
R3479 T14280 T14265 punct .,assessed
R3480 T14282 T14283 det The,females
R3481 T14283 T14286 nsubj females,showed
R3482 T14284 T14283 amod adult,females
R3483 T14285 T14283 amod heterozygous,females
R3484 T14287 T14288 det a,tendency
R3485 T14288 T14286 dobj tendency,showed
R3486 T14289 T14288 amod mild,tendency
R3487 T14290 T14288 prep toward,tendency
R3488 T14291 T14292 amod increased,output
R3489 T14292 T14290 pobj output,toward
R3490 T14293 T14292 amod urinary,output
R3491 T14294 T14292 cc and,output
R3492 T14295 T14296 compound water,intake
R3493 T14296 T14292 conj intake,output
R3494 T14297 T14292 cc and,output
R3495 T14298 T14299 amod decreased,osmolality
R3496 T14299 T14292 conj osmolality,output
R3497 T14300 T14299 compound urine,osmolality
R3498 T14301 T14286 punct .,showed
R3499 T14303 T14304 nsubjpass Knockout,reported
R3500 T14305 T14303 prep of,Knockout
R3501 T14306 T14307 det the,gene
R3502 T14307 T14305 pobj gene,of
R3503 T14308 T14307 compound mouse,gene
R3504 T14309 T14307 compound Aqp2,gene
R3505 T14310 T14304 aux has,reported
R3506 T14311 T14304 neg not,reported
R3507 T14312 T14304 auxpass been,reported
R3508 T14313 T14304 punct .,reported
R3509 T14315 T14316 det A,in
R3510 T14316 T14319 nsubjpass in,made
R3511 T14317 T14316 compound knock,in
R3512 T14318 T14316 punct -,in
R3513 T14320 T14316 prep of,in
R3514 T14321 T14322 det a,mutation
R3515 T14322 T14320 pobj mutation,of
R3516 T14323 T14322 amod human,mutation
R3517 T14324 T14325 npadvmod disease,causing
R3518 T14325 T14322 amod causing,mutation
R3519 T14326 T14325 punct -,causing
R3520 T14327 T14322 punct (,mutation
R3521 T14328 T14322 appos T126M,mutation
R3522 T14329 T14322 punct ),mutation
R3523 T14330 T14319 punct ", ",made
R3524 T14331 T14319 advmod however,made
R3525 T14332 T14319 punct ", ",made
R3526 T14333 T14319 aux has,made
R3527 T14334 T14319 auxpass been,made
R3528 T14335 T14336 punct [,18
R3529 T14336 T14319 parataxis 18,made
R3530 T14337 T14336 punct ],18
R3531 T14338 T14319 punct .,made
R3532 T14340 T14341 det These,mice
R3533 T14341 T14342 nsubj mice,have
R3534 T14343 T14344 det a,defect
R3535 T14344 T14342 dobj defect,have
R3536 T14345 T14344 amod severe,defect
R3537 T14346 T14347 npadvmod urine,concentrating
R3538 T14347 T14344 amod concentrating,defect
R3539 T14348 T14347 punct -,concentrating
R3540 T14349 T14344 acl resulting,defect
R3541 T14350 T14349 prep in,resulting
R3542 T14351 T14350 pobj dehydration,in
R3543 T14352 T14351 cc and,dehydration
R3544 T14353 T14351 conj death,dehydration
R3545 T14354 T14349 prep within,resulting
R3546 T14355 T14356 nummod 1,wk
R3547 T14356 T14354 pobj wk,within
R3548 T14357 T14356 prep of,wk
R3549 T14358 T14357 pobj birth,of
R3550 T14359 T14342 punct .,have
R3551 T14361 T14362 advmod Curiously,localize
R3552 T14363 T14362 punct ", ",localize
R3553 T14364 T14365 compound AQP2,T126M
R3554 T14365 T14362 nsubj T126M,localize
R3555 T14366 T14365 punct -,T126M
R3556 T14367 T14362 aux does,localize
R3557 T14368 T14362 advmod properly,localize
R3558 T14369 T14362 prep in,localize
R3559 T14370 T14371 advmod at,least
R3560 T14371 T14372 advmod least,subset
R3561 T14372 T14369 pobj subset,in
R3562 T14373 T14372 det a,subset
R3563 T14374 T14372 prep of,subset
R3564 T14375 T14374 pobj cells,of
R3565 T14376 T14362 punct .,localize
R3566 T14378 T14379 det The,morphology
R3567 T14379 T14384 nsubj morphology,makes
R3568 T14380 T14381 advmod grossly,abnormal
R3569 T14381 T14379 amod abnormal,morphology
R3570 T14382 T14383 amod collecting,duct
R3571 T14383 T14379 compound duct,morphology
R3572 T14385 T14386 nsubj it,impossible
R3573 T14386 T14384 ccomp impossible,makes
R3574 T14387 T14388 aux to,pinpoint
R3575 T14388 T14386 advcl pinpoint,impossible
R3576 T14389 T14390 det the,defect
R3577 T14390 T14388 dobj defect,pinpoint
R3578 T14391 T14390 amod molecular,defect
R3579 T14392 T14388 prep in,pinpoint
R3580 T14393 T14394 det these,mice
R3581 T14394 T14392 pobj mice,in
R3582 T14395 T14394 amod knock,mice
R3583 T14396 T14395 punct -,knock
R3584 T14397 T14395 prt in,knock
R3585 T14398 T14384 punct .,makes
R3586 T14400 T14401 det The,in
R3587 T14401 T14405 nsubj in,shows
R3588 T14402 T14401 compound T126M,in
R3589 T14403 T14401 compound knock,in
R3590 T14404 T14401 punct -,in
R3591 T14406 T14405 advmod clearly,shows
R3592 T14407 T14408 mark that,is
R3593 T14408 T14405 ccomp is,shows
R3594 T14409 T14408 nsubj Aqp2,is
R3595 T14410 T14411 det an,gene
R3596 T14411 T14408 attr gene,is
R3597 T14412 T14411 amod essential,gene
R3598 T14413 T14414 punct [,18
R3599 T14414 T14405 parataxis 18,shows
R3600 T14415 T14414 punct ],18
R3601 T14416 T14405 punct .,shows
R3602 T14418 T14419 det The,fact
R3603 T14419 T14420 nsubj fact,shows
R3604 T14421 T14422 mark that,survive
R3605 T14422 T14419 acl survive,fact
R3606 T14423 T14424 poss our,mice
R3607 T14424 T14422 nsubj mice,survive
R3608 T14425 T14426 preconj either,possesses
R3609 T14426 T14420 advcl possesses,shows
R3610 T14427 T14426 mark that,possesses
R3611 T14428 T14429 compound AQP2,F204V
R3612 T14429 T14426 nsubj F204V,possesses
R3613 T14430 T14429 punct -,F204V
R3614 T14431 T14432 det some,ability
R3615 T14432 T14426 dobj ability,possesses
R3616 T14433 T14432 amod residual,ability
R3617 T14434 T14435 npadvmod water,transporting
R3618 T14435 T14432 amod transporting,ability
R3619 T14436 T14426 cc or,possesses
R3620 T14437 T14438 mark that,are
R3621 T14438 T14426 conj are,possesses
R3622 T14439 T14438 expl there,are
R3623 T14440 T14441 npadvmod AVP,independent
R3624 T14441 T14443 amod independent,pathways
R3625 T14442 T14441 punct -,independent
R3626 T14443 T14438 attr pathways,are
R3627 T14444 T14443 prep for,pathways
R3628 T14445 T14446 compound water,reabsorption
R3629 T14446 T14444 pobj reabsorption,for
R3630 T14447 T14420 punct .,shows
R3631 T14449 T14450 amod Residual,activity
R3632 T14450 T14451 nsubj activity,is
R3633 T14452 T14450 prep of,activity
R3634 T14453 T14454 compound AQP2,F204V
R3635 T14454 T14452 pobj F204V,of
R3636 T14455 T14454 punct -,F204V
R3637 T14456 T14451 acomp likely,is
R3638 T14457 T14451 punct ", ",is
R3639 T14458 T14459 mark as,show
R3640 T14459 T14451 advcl show,is
R3641 T14460 T14461 compound mutant,animals
R3642 T14461 T14459 nsubj animals,show
R3643 T14462 T14463 det some,response
R3644 T14463 T14459 dobj response,show
R3645 T14464 T14463 amod small,response
R3646 T14465 T14463 prep to,response
R3647 T14466 T14465 pobj dDAVP,to
R3648 T14467 T14459 punct ", ",show
R3649 T14468 T14469 mark although,remains
R3650 T14469 T14459 advcl remains,show
R3651 T14470 T14471 npadvmod dDAVP,stimulated
R3652 T14471 T14473 amod stimulated,osmolality
R3653 T14472 T14471 punct -,stimulated
R3654 T14473 T14469 nsubj osmolality,remains
R3655 T14474 T14473 compound urine,osmolality
R3656 T14475 T14476 advmod quite,low
R3657 T14476 T14469 acomp low,remains
R3658 T14477 T14451 punct .,is
R3659 T14479 T14480 nsubj Immunostaining,shows
R3660 T14481 T14479 prep of,Immunostaining
R3661 T14482 T14481 pobj kidney,of
R3662 T14483 T14484 mark that,transport
R3663 T14484 T14480 ccomp transport,shows
R3664 T14485 T14486 compound AQP2,F204V
R3665 T14486 T14484 nsubj F204V,transport
R3666 T14487 T14486 punct -,F204V
R3667 T14488 T14484 aux does,transport
R3668 T14489 T14484 neg not,transport
R3669 T14490 T14484 advmod efficiently,transport
R3670 T14491 T14484 dobj water,transport
R3671 T14492 T14484 punct ", ",transport
R3672 T14493 T14494 mark because,fails
R3673 T14494 T14484 advcl fails,transport
R3674 T14495 T14494 nsubj it,fails
R3675 T14496 T14497 aux to,localize
R3676 T14497 T14494 xcomp localize,fails
R3677 T14498 T14497 prep to,localize
R3678 T14499 T14500 det the,surface
R3679 T14500 T14498 pobj surface,to
R3680 T14501 T14500 amod apical,surface
R3681 T14502 T14500 compound cell,surface
R3682 T14503 T14497 prep after,localize
R3683 T14504 T14505 compound dDAVP,treatment
R3684 T14505 T14503 pobj treatment,after
R3685 T14506 T14480 punct .,shows
R3686 T14508 T14509 det Some,activity
R3687 T14509 T14511 nsubj activity,imply
R3688 T14510 T14509 amod residual,activity
R3689 T14512 T14509 prep of,activity
R3690 T14513 T14512 pobj AQP2,of
R3691 T14514 T14511 aux would,imply
R3692 T14515 T14516 mark that,getting
R3693 T14516 T14511 ccomp getting,imply
R3694 T14517 T14518 det some,portion
R3695 T14518 T14516 nsubj portion,getting
R3696 T14519 T14518 amod small,portion
R3697 T14520 T14518 punct ", ",portion
R3698 T14521 T14518 amod undetectable,portion
R3699 T14522 T14518 prep of,portion
R3700 T14523 T14524 det the,protein
R3701 T14524 T14522 pobj protein,of
R3702 T14525 T14524 compound mutant,protein
R3703 T14526 T14516 aux is,getting
R3704 T14527 T14516 prep to,getting
R3705 T14528 T14529 det the,surface
R3706 T14529 T14527 pobj surface,to
R3707 T14530 T14529 compound cell,surface
R3708 T14531 T14511 punct .,imply
R3709 T14533 T14534 det The,experiment
R3710 T14534 T14537 nsubj experiment,suggests
R3711 T14535 T14534 compound surface,experiment
R3712 T14536 T14534 compound biotinylation,experiment
R3713 T14538 T14539 punct (,Figure
R3714 T14539 T14534 parataxis Figure,experiment
R3715 T14540 T14539 nummod 5D,Figure
R3716 T14541 T14539 punct ),Figure
R3717 T14542 T14543 mark that,gets
R3718 T14543 T14537 ccomp gets,suggests
R3719 T14544 T14545 det no,protein
R3720 T14545 T14543 nsubj protein,gets
R3721 T14546 T14545 compound mutant,protein
R3722 T14547 T14543 prep to,gets
R3723 T14548 T14549 det the,surface
R3724 T14549 T14547 pobj surface,to
R3725 T14550 T14537 punct ", ",suggests
R3726 T14551 T14537 cc but,suggests
R3727 T14552 T14553 nsubj this,reflect
R3728 T14553 T14537 conj reflect,suggests
R3729 T14554 T14553 aux does,reflect
R3730 T14555 T14553 neg not,reflect
R3731 T14556 T14553 advmod necessarily,reflect
R3732 T14557 T14558 det the,situation
R3733 T14558 T14553 dobj situation,reflect
R3734 T14559 T14560 advmod in,vivo
R3735 T14560 T14558 advmod vivo,situation
R3736 T14561 T14553 punct .,reflect
R3737 T14563 T14564 mark While,be
R3738 T14564 T14572 advcl be,shows
R3739 T14565 T14566 det this,fraction
R3740 T14566 T14564 nsubj fraction,be
R3741 T14567 T14566 amod small,fraction
R3742 T14568 T14566 prep of,fraction
R3743 T14569 T14568 pobj protein,of
R3744 T14570 T14564 aux may,be
R3745 T14571 T14564 neg not,be
R3746 T14573 T14564 acomp detectable,be
R3747 T14574 T14564 prep by,be
R3748 T14575 T14574 pobj immunofluorescence,by
R3749 T14576 T14572 punct ", ",shows
R3750 T14577 T14578 compound Western,blotting
R3751 T14578 T14572 nsubj blotting,shows
R3752 T14579 T14580 mark that,progress
R3753 T14580 T14572 ccomp progress,shows
R3754 T14581 T14582 det some,protein
R3755 T14582 T14580 nsubj protein,progress
R3756 T14583 T14582 compound mutant,protein
R3757 T14584 T14580 aux does,progress
R3758 T14585 T14580 prep beyond,progress
R3759 T14586 T14587 det the,ER
R3760 T14587 T14585 pobj ER,beyond
R3761 T14588 T14589 punct (,species
R3762 T14589 T14572 parataxis species,shows
R3763 T14590 T14591 quantmod 35,45
R3764 T14591 T14593 nummod 45,kDa
R3765 T14592 T14591 punct –,45
R3766 T14593 T14589 compound kDa,species
R3767 T14594 T14589 prep in,species
R3768 T14595 T14594 pobj Figure,in
R3769 T14596 T14595 nummod 3A,Figure
R3770 T14597 T14589 punct ),species
R3771 T14598 T14572 punct .,shows
R3772 T14600 T14601 prep Compared,is
R3773 T14602 T14600 prep to,Compared
R3774 T14603 T14604 amod wild,type
R3775 T14604 T14602 pobj type,to
R3776 T14605 T14604 punct -,type
R3777 T14606 T14601 punct ", ",is
R3778 T14607 T14608 compound mutant,protein
R3779 T14608 T14601 nsubj protein,is
R3780 T14609 T14601 acomp enriched,is
R3781 T14610 T14609 prep in,enriched
R3782 T14611 T14612 det the,form
R3783 T14612 T14610 pobj form,in
R3784 T14613 T14614 amod high,mannose
R3785 T14614 T14612 nmod mannose,form
R3786 T14615 T14614 punct -,mannose
R3787 T14616 T14612 punct ", ",form
R3788 T14617 T14618 npadvmod core,glycosylated
R3789 T14618 T14612 amod glycosylated,form
R3790 T14619 T14618 punct -,glycosylated
R3791 T14620 T14621 punct (,kDa
R3792 T14621 T14612 parataxis kDa,form
R3793 T14622 T14621 nummod 31,kDa
R3794 T14623 T14621 punct ),kDa
R3795 T14624 T14609 cc and,enriched
R3796 T14625 T14609 conj deficient,enriched
R3797 T14626 T14625 prep in,deficient
R3798 T14627 T14628 amod nonglycosylated,forms
R3799 T14628 T14626 pobj forms,in
R3800 T14629 T14630 punct (,kDa
R3801 T14630 T14627 parataxis kDa,nonglycosylated
R3802 T14631 T14630 nummod 29,kDa
R3803 T14632 T14630 punct ),kDa
R3804 T14633 T14627 cc and,nonglycosylated
R3805 T14634 T14635 amod complex,glycosylated
R3806 T14635 T14627 conj glycosylated,nonglycosylated
R3807 T14636 T14637 punct (,kDa
R3808 T14637 T14635 parataxis kDa,glycosylated
R3809 T14638 T14639 quantmod 35,45
R3810 T14639 T14637 nummod 45,kDa
R3811 T14640 T14639 punct –,45
R3812 T14641 T14637 punct ),kDa
R3813 T14642 T14601 punct .,is
R3814 T14644 T14645 det The,presence
R3815 T14645 T14646 nsubj presence,indicates
R3816 T14647 T14645 prep of,presence
R3817 T14648 T14649 det a,amount
R3818 T14649 T14647 pobj amount,of
R3819 T14650 T14649 amod reduced,amount
R3820 T14651 T14650 cc but,reduced
R3821 T14652 T14650 conj detectable,reduced
R3822 T14653 T14649 prep of,amount
R3823 T14654 T14653 pobj protein,of
R3824 T14655 T14654 prep in,protein
R3825 T14656 T14657 det the,range
R3826 T14657 T14655 pobj range,in
R3827 T14658 T14659 quantmod 35,45
R3828 T14659 T14661 nummod 45,kDa
R3829 T14660 T14659 punct –,45
R3830 T14661 T14657 compound kDa,range
R3831 T14662 T14663 mark that,transported
R3832 T14663 T14646 ccomp transported,indicates
R3833 T14664 T14665 compound mutant,protein
R3834 T14665 T14663 nsubjpass protein,transported
R3835 T14666 T14663 auxpass is,transported
R3836 T14667 T14663 prep out,transported
R3837 T14668 T14667 prep of,out
R3838 T14669 T14670 det the,ER
R3839 T14670 T14668 pobj ER,of
R3840 T14671 T14663 punct ", ",transported
R3841 T14672 T14673 cc but,with
R3842 T14673 T14663 prep with,transported
R3843 T14674 T14675 advmod greatly,reduced
R3844 T14675 T14676 amod reduced,efficiency
R3845 T14676 T14673 pobj efficiency,with
R3846 T14677 T14646 punct .,indicates
R3847 T14679 T14680 nsubj Colocalization,shows
R3848 T14681 T14679 prep of,Colocalization
R3849 T14682 T14683 compound AQP2,F204V
R3850 T14683 T14681 pobj F204V,of
R3851 T14684 T14683 punct -,F204V
R3852 T14685 T14679 prep with,Colocalization
R3853 T14686 T14687 det the,protein
R3854 T14687 T14685 pobj protein,with
R3855 T14688 T14687 compound ER,protein
R3856 T14689 T14687 appos calnexin,protein
R3857 T14690 T14679 prep in,Colocalization
R3858 T14691 T14692 amod transfected,cells
R3859 T14692 T14690 pobj cells,in
R3860 T14693 T14692 compound MDCK,cells
R3861 T14694 T14695 mark that,progress
R3862 T14695 T14680 ccomp progress,shows
R3863 T14696 T14695 punct ", ",progress
R3864 T14697 T14698 mark while,trapped
R3865 T14698 T14695 advcl trapped,progress
R3866 T14738 T14737 prep of,fraction
R3867 T14699 T14698 nsubjpass most,trapped
R3868 T14739 T14740 compound mutant,protein
R3869 T14700 T14699 prep of,most
R3870 T14740 T14738 pobj protein,of
R3871 T14741 T14734 prep beyond,transport
R3872 T14701 T14702 det the,protein
R3873 T14742 T14743 det the,ER
R3874 T14743 T14741 pobj ER,beyond
R3875 T14744 T14734 prep in,transport
R3876 T14702 T14700 pobj protein,of
R3877 T14745 T14746 compound MDCK,cells
R3878 T14746 T14744 pobj cells,in
R3879 T14747 T14718 advmod all,are
R3880 T14703 T14702 compound mutant,protein
R3881 T14748 T14718 acomp consistent,are
R3882 T14749 T14748 prep with,consistent
R3883 T14750 T14751 det the,notion
R3884 T14704 T14698 auxpass is,trapped
R3885 T14751 T14749 pobj notion,with
R3886 T14752 T14753 mark that,is
R3887 T14753 T14751 advcl is,notion
R3888 T14705 T14698 prep in,trapped
R3889 T14754 T14755 compound AQP2,F204V
R3890 T14755 T14757 compound F204V,misfolding
R3891 T14756 T14755 punct -,F204V
R3892 T14706 T14707 det the,ER
R3893 T14757 T14753 nsubj misfolding,is
R3894 T14758 T14753 acomp limited,is
R3895 T14759 T14753 cc and,is
R3896 T14760 T14761 mark that,retain
R3897 T14761 T14753 conj retain,is
R3898 T14762 T14761 nsubj it,retain
R3899 T14707 T14705 pobj ER,in
R3900 T14763 T14761 aux may,retain
R3901 T14764 T14765 det some,activity
R3902 T14765 T14761 dobj activity,retain
R3903 T14708 T14695 punct ", ",progress
R3904 T14766 T14765 amod residual,activity
R3905 T14767 T14768 npadvmod water,transporting
R3906 T14709 T14695 nsubj some,progress
R3907 T14768 T14765 amod transporting,activity
R3908 T14769 T14718 punct .,are
R3909 T14771 T14772 advmod Evidently,is
R3910 T14710 T14695 aux does,progress
R3911 T14773 T14774 det this,activity
R3912 T14774 T14772 nsubj activity,is
R3913 T14711 T14695 prep beyond,progress
R3914 T14775 T14774 amod residual,activity
R3915 T14712 T14713 det the,ER
R3916 T14776 T14772 acomp sufficient,is
R3917 T14777 T14776 prep for,sufficient
R3918 T14713 T14711 pobj ER,beyond
R3919 T14778 T14779 det the,viability
R3920 T14779 T14777 pobj viability,for
R3921 T14780 T14779 cc and,viability
R3922 T14714 T14680 punct .,shows
R3923 T14781 T14779 conj growth,viability
R3924 T14782 T14779 prep of,viability
R3925 T14783 T14784 compound mutant,animals
R3926 T14716 T14717 amod Diminished,response
R3927 T14784 T14782 pobj animals,of
R3928 T14785 T14772 punct .,is
R3929 T14787 T14788 amod Reduced,efficiency
R3930 T14717 T14718 nsubj response,are
R3931 T14788 T14789 nsubj efficiency,explain
R3932 T14790 T14788 prep in,efficiency
R3933 T14719 T14717 prep to,response
R3934 T14791 T14790 pcomp exiting,in
R3935 T14792 T14793 det the,ER
R3936 T14793 T14791 dobj ER,exiting
R3937 T14794 T14789 aux may,explain
R3938 T14720 T14719 pobj dDAVP,to
R3939 T14795 T14796 advmod why,enriched
R3940 T14796 T14789 ccomp enriched,explain
R3941 T14797 T14798 compound AQP2,F204V
R3942 T14721 T14717 punct ", ",response
R3943 T14798 T14796 nsubjpass F204V,enriched
R3944 T14799 T14798 punct -,F204V
R3945 T14800 T14796 auxpass is,enriched
R3946 T14801 T14796 prep in,enriched
R3947 T14802 T14803 det the,form
R3948 T14803 T14801 pobj form,in
R3949 T14722 T14723 amod diminished,abundance
R3950 T14804 T14805 nummod 31,kDa
R3951 T14805 T14803 nmod kDa,form
R3952 T14806 T14807 amod high,mannose
R3953 T14807 T14803 nmod mannose,form
R3954 T14723 T14717 conj abundance,response
R3955 T14808 T14807 punct -,mannose
R3956 T14809 T14803 amod glycosylated,form
R3957 T14810 T14789 punct .,explain
R3958 T14724 T14723 prep of,abundance
R3959 T14725 T14726 amod mature,protein
R3960 T14812 T14813 det The,oligosaccharide
R3961 T14813 T14818 nsubjpass oligosaccharide,added
R3962 T14814 T14815 amod high,mannose
R3963 T14815 T14813 compound mannose,oligosaccharide
R3964 T14816 T14815 punct -,mannose
R3965 T14726 T14724 pobj protein,of
R3966 T14817 T14813 compound core,oligosaccharide
R3967 T14727 T14726 amod glycosylated,protein
R3968 T14819 T14818 auxpass is,added
R3969 T14820 T14818 prep in,added
R3970 T14821 T14822 det the,ER
R3971 T14728 T14723 prep in,abundance
R3972 T14822 T14820 pobj ER,in
R3973 T14823 T14818 cc and,added
R3974 T14824 T14825 auxpass is,modified
R3975 T14729 T14730 compound mutant,animals
R3976 T14825 T14818 conj modified,added
R3977 T14826 T14825 advmod later,modified
R3978 T14730 T14728 pobj animals,in
R3979 T14827 T14825 cc and,modified
R3980 T14828 T14825 conj elaborated,modified
R3981 T14829 T14828 prep in,elaborated
R3982 T14731 T14723 punct ", ",abundance
R3983 T14830 T14831 det the,apparatus
R3984 T14831 T14829 pobj apparatus,in
R3985 T14732 T14723 cc and,abundance
R3986 T14832 T14831 compound Golgi,apparatus
R3987 T14833 T14834 punct [,26
R3988 T14834 T14825 parataxis 26,modified
R3989 T14733 T14734 det the,transport
R3990 T14835 T14834 punct ],26
R3991 T14836 T14818 punct .,added
R3992 T14734 T14723 conj transport,abundance
R3993 T14838 T14839 det The,increase
R3994 T14839 T14840 nsubj increase,reflect
R3995 T14735 T14734 prep of,transport
R3996 T14841 T14839 prep in,increase
R3997 T14842 T14843 det the,form
R3998 T14843 T14841 pobj form,in
R3999 T14736 T14737 det a,fraction
R4000 T14737 T14735 pobj fraction,of
R4001 T14950 T14949 aux can,explain
R4002 T14844 T14845 amod high,mannose
R4003 T14845 T14843 nmod mannose,form
R4004 T14846 T14845 punct -,mannose
R4005 T14847 T14843 amod glycosylated,form
R4006 T14951 T14949 neg not,explain
R4007 T14848 T14843 prep of,form
R4008 T14849 T14850 compound AQP2,F204V
R4009 T14850 T14848 pobj F204V,of
R4010 T14952 T14953 det the,lack
R4011 T14851 T14850 punct -,F204V
R4012 T14852 T14840 aux may,reflect
R4013 T14853 T14840 advmod simply,reflect
R4014 T14953 T14949 dobj lack,explain
R4015 T14854 T14855 poss its,presence
R4016 T14954 T14953 prep of,lack
R4017 T14855 T14840 dobj presence,reflect
R4018 T14856 T14855 amod prolonged,presence
R4019 T14857 T14855 prep in,presence
R4020 T14858 T14859 det the,ER
R4021 T14955 T14956 det any,protein
R4022 T14859 T14857 pobj ER,in
R4023 T14860 T14855 cc and,presence
R4024 T14861 T14855 conj exposure,presence
R4025 T14956 T14954 pobj protein,of
R4026 T14957 T14958 npadvmod ER,retained
R4027 T14862 T14861 prep to,exposure
R4028 T14863 T14864 compound oligosaccharyl,transferase
R4029 T14864 T14862 pobj transferase,to
R4030 T14958 T14956 amod retained,protein
R4031 T14865 T14840 punct .,reflect
R4032 T14867 T14868 mark While,explains
R4033 T14959 T14958 punct -,retained
R4034 T14868 T14873 advcl explains,is
R4035 T14960 T14956 compound mutant,protein
R4036 T14869 T14870 amod improper,localization
R4037 T14870 T14868 nsubj localization,explains
R4038 T14871 T14870 prep of,localization
R4039 T14961 T14949 punct .,explain
R4040 T14872 T14871 pobj AQP2,of
R4041 T14963 T14964 advmod Rather,suggests
R4042 T14874 T14875 det the,phenotype
R4043 T14875 T14868 dobj phenotype,explains
R4044 T14876 T14875 prep of,phenotype
R4045 T14877 T14878 amod homozygous,mice
R4046 T14878 T14876 pobj mice,of
R4047 T14965 T14964 punct ", ",suggests
R4048 T14879 T14878 compound mutant,mice
R4049 T14880 T14873 punct ", ",is
R4050 T14881 T14882 det the,lack
R4051 T14882 T14873 nsubj lack,is
R4052 T14883 T14882 amod complete,lack
R4053 T14884 T14882 prep of,lack
R4054 T14885 T14886 det a,phenotype
R4055 T14886 T14884 pobj phenotype,of
R4056 T14966 T14967 det the,phenotype
R4057 T14887 T14882 prep in,lack
R4058 T14888 T14889 amod heterozygous,mice
R4059 T14889 T14887 pobj mice,in
R4060 T14967 T14964 nsubj phenotype,suggests
R4061 T14890 T14891 advmod more,difficult
R4062 T14891 T14873 acomp difficult,is
R4063 T14892 T14893 aux to,explain
R4064 T14968 T14967 prep of,phenotype
R4065 T14893 T14891 advcl explain,difficult
R4066 T14894 T14873 punct .,is
R4067 T14969 T14970 det the,animals
R4068 T14896 T14897 advmod Physiologically,have
R4069 T14970 T14968 pobj animals,of
R4070 T14898 T14897 punct ", ",have
R4071 T14899 T14900 amod heterozygous,mice
R4072 T14900 T14897 nsubj mice,have
R4073 T14971 T14970 nmod Aqp2F204V,animals
R4074 T14901 T14902 det no,symptoms
R4075 T14902 T14897 dobj symptoms,have
R4076 T14903 T14904 punct (,see
R4077 T14972 T14971 punct /,Aqp2F204V
R4078 T14904 T14897 parataxis see,have
R4079 T14905 T14904 dobj Figure,see
R4080 T14973 T14971 punct +,Aqp2F204V
R4081 T14906 T14905 nummod 1C,Figure
R4082 T14907 T14904 punct ),see
R4083 T14908 T14897 punct ", ",have
R4084 T14909 T14897 cc and,have
R4085 T14974 T14975 mark that,rescued
R4086 T14910 T14911 nsubj they,are
R4087 T14911 T14897 conj are,have
R4088 T14975 T14964 ccomp rescued,suggests
R4089 T14912 T14911 acomp indistinguishable,are
R4090 T14913 T14912 prep from,indistinguishable
R4091 T14914 T14915 amod wild,type
R4092 T14976 T14977 det the,protein
R4093 T14915 T14913 pobj type,from
R4094 T14916 T14911 prep on,are
R4095 T14917 T14916 pobj immunostaining,on
R4096 T14977 T14975 nsubjpass protein,rescued
R4097 T14918 T14917 prep of,immunostaining
R4098 T14919 T14918 pobj kidneys,of
R4099 T14920 T14921 punct (,Figure
R4100 T14978 T14977 compound mutant,protein
R4101 T14921 T14911 parataxis Figure,are
R4102 T14922 T14921 nummod 5A,Figure
R4103 T14923 T14921 punct ),Figure
R4104 T14924 T14911 punct .,are
R4105 T14979 T14975 aux is,rescued
R4106 T14926 T14927 det The,presence
R4107 T14927 T14928 nsubj presence,explain
R4108 T14980 T14975 auxpass being,rescued
R4109 T14929 T14927 prep of,presence
R4110 T14930 T14931 nummod 50,%
R4111 T14931 T14929 pobj %,of
R4112 T14981 T14975 agent by,rescued
R4113 T14932 T14931 prep of,%
R4114 T14933 T14934 det the,amount
R4115 T14934 T14932 pobj amount,of
R4116 T14982 T14983 det the,protein
R4117 T14935 T14934 amod normal,amount
R4118 T14936 T14934 prep of,amount
R4119 T14937 T14938 amod wild,type
R4120 T14938 T14940 compound type,protein
R4121 T14983 T14981 pobj protein,by
R4122 T14939 T14938 punct -,type
R4123 T14940 T14936 pobj protein,of
R4124 T14984 T14985 amod wild,type
R4125 T14941 T14928 aux may,explain
R4126 T14942 T14943 det the,lack
R4127 T14943 T14928 dobj lack,explain
R4128 T14944 T14943 prep of,lack
R4129 T14985 T14983 compound type,protein
R4130 T14945 T14944 pobj symptoms,of
R4131 T14946 T14928 punct ", ",explain
R4132 T14947 T14928 cc but,explain
R4133 T14986 T14985 punct -,type
R4134 T14948 T14949 nsubj it,explain
R4135 T14987 T14964 punct .,suggests
R4136 T14949 T14928 conj explain,explain
R4137 T14989 T14990 advmod Indeed,demonstrated
R4138 T14991 T14990 punct ", ",demonstrated
R4139 T14992 T14993 compound de,Mattia
R4140 T14993 T14990 nsubj Mattia,demonstrated
R4141 T14994 T14995 advmod et,al.
R4142 T15056 T15055 pobj this,of
R4143 T15057 T15053 punct ", ",interact
R4144 T14995 T14993 advmod al.,Mattia
R4145 T15058 T15059 compound AQP2,F204V
R4146 T15059 T15053 nsubj F204V,interact
R4147 T15060 T15059 punct -,F204V
R4148 T14996 T14997 punct (,26
R4149 T15061 T15053 aux can,interact
R4150 T15062 T15053 prep with,interact
R4151 T14997 T14993 parataxis 26,Mattia
R4152 T15063 T15064 amod wild,type
R4153 T15064 T15066 compound type,AQP2
R4154 T15065 T15064 punct -,type
R4155 T15066 T15062 pobj AQP2,with
R4156 T15067 T15068 punct (,Figure
R4157 T15068 T15053 parataxis Figure,interact
R4158 T14998 T14997 punct ),26
R4159 T15069 T15068 nummod 5B,Figure
R4160 T15070 T15068 punct ),Figure
R4161 T15071 T15053 punct ", ",interact
R4162 T15072 T15053 cc and,interact
R4163 T14999 T14990 aux have,demonstrated
R4164 T15073 T15074 advmod when,coexpressed
R4165 T15074 T15075 advcl coexpressed,reach
R4166 T15000 T15001 mark that,translocate
R4167 T15075 T15053 conj reach,interact
R4168 T15076 T15074 prep with,coexpressed
R4169 T15077 T15078 amod wild,type
R4170 T15001 T14990 advcl translocate,demonstrated
R4171 T15078 T15080 compound type,protein
R4172 T15079 T15078 punct -,type
R4173 T15080 T15076 pobj protein,with
R4174 T15002 T15003 nummod one,allele
R4175 T15081 T15075 punct ", ",reach
R4176 T15082 T15083 compound AQP2,F204V
R4177 T15083 T15075 nsubj F204V,reach
R4178 T15003 T15001 nsubj allele,translocate
R4179 T15084 T15083 punct -,F204V
R4180 T15085 T15075 aux can,reach
R4181 T15086 T15087 det the,surface
R4182 T15004 T15003 amod recessive,allele
R4183 T15087 T15075 dobj surface,reach
R4184 T15088 T15087 compound cell,surface
R4185 T15089 T15090 punct (,panel
R4186 T15005 T15003 prep of,allele
R4187 T15090 T15075 parataxis panel,reach
R4188 T15091 T15090 nmod Figure,panel
R4189 T15092 T15091 nummod 5C,Figure
R4190 T15093 T15094 npadvmod bottom,right
R4191 T15006 T15005 pobj Aqp2,of
R4192 T15094 T15090 amod right,panel
R4193 T15095 T15090 cc and,panel
R4194 T15096 T15090 conj 5D,panel
R4195 T15007 T15003 punct ", ",allele
R4196 T15097 T15090 punct ),panel
R4197 T15098 T15053 punct .,interact
R4198 T15008 T15003 appos P262L,allele
R4199 T15100 T15101 mark Although,demonstrated
R4200 T15101 T15105 advcl demonstrated,show
R4201 T15009 T15001 punct ", ",translocate
R4202 T15102 T15101 nsubjpass it,demonstrated
R4203 T15103 T15101 aux has,demonstrated
R4204 T15104 T15101 auxpass been,demonstrated
R4205 T15010 T15001 aux does,translocate
R4206 T15106 T15107 mark that,fails
R4207 T15107 T15101 ccomp fails,demonstrated
R4208 T15108 T15109 det a,allele
R4209 T15109 T15107 nsubj allele,fails
R4210 T15110 T15109 amod recessive,allele
R4211 T15011 T15001 neg not,translocate
R4212 T15111 T15109 punct (,allele
R4213 T15112 T15109 acl encoding,allele
R4214 T15113 T15114 compound AQP2,R187C
R4215 T15012 T15001 advmod properly,translocate
R4216 T15114 T15112 dobj R187C,encoding
R4217 T15115 T15114 punct -,R187C
R4218 T15116 T15109 punct ),allele
R4219 T15013 T15014 advmod when,expressed
R4220 T15117 T15109 prep of,allele
R4221 T15118 T15117 pobj NDI,of
R4222 T15014 T15001 advcl expressed,translocate
R4223 T15119 T15120 aux to,interact
R4224 T15015 T15014 prep by,expressed
R4225 T15120 T15107 xcomp interact,fails
R4226 T15121 T15120 prep with,interact
R4227 T15122 T15123 amod wild,type
R4228 T15016 T15015 pobj itself,by
R4229 T15123 T15125 compound type,AQP2
R4230 T15124 T15123 punct -,type
R4231 T15125 T15121 pobj AQP2,with
R4232 T15017 T15014 prep in,expressed
R4233 T15126 T15127 punct [,6
R4234 T15127 T15101 parataxis 6,demonstrated
R4235 T15018 T15019 compound MDCK,cells
R4236 T15128 T15127 punct ],6
R4237 T15129 T15105 punct ", ",show
R4238 T15019 T15017 pobj cells,in
R4239 T15130 T15105 advmod here,show
R4240 T15131 T15105 nsubj we,show
R4241 T15132 T15133 mark that,interact
R4242 T15133 T15105 ccomp interact,show
R4243 T15020 T15001 punct ", ",translocate
R4244 T15134 T15135 compound AQP2,F204V
R4245 T15135 T15133 nsubj F204V,interact
R4246 T15021 T15001 cc but,translocate
R4247 T15136 T15135 punct -,F204V
R4248 T15137 T15133 aux does,interact
R4249 T15138 T15133 prep with,interact
R4250 T15022 T15023 mark that,localizes
R4251 T15139 T15140 det the,protein
R4252 T15140 T15138 pobj protein,with
R4253 T15141 T15142 amod wild,type
R4254 T15023 T15001 conj localizes,translocate
R4255 T15142 T15140 compound type,protein
R4256 T15143 T15142 punct -,type
R4257 T15144 T15133 punct ", ",interact
R4258 T15145 T15146 advmod presumably,as
R4259 T15146 T15133 prep as,interact
R4260 T15147 T15146 pobj part,as
R4261 T15024 T15023 prep in,localizes
R4262 T15148 T15147 prep of,part
R4263 T15149 T15148 pobj heterotetramers,of
R4264 T15025 T15026 det the,presence
R4265 T15150 T15133 punct ", ",interact
R4266 T15151 T15133 cc and,interact
R4267 T15152 T15133 conj represents,interact
R4268 T15153 T15154 det a,allele
R4269 T15026 T15024 pobj presence,in
R4270 T15154 T15152 dobj allele,represents
R4271 T15155 T15154 amod rescuable,allele
R4272 T15156 T15152 punct ", ",represents
R4273 T15027 T15026 prep of,presence
R4274 T15157 T15158 preconj both,vitro
R4275 T15158 T15152 advmod vitro,represents
R4276 T15028 T15029 amod wild,type
R4277 T15159 T15158 advmod in,vitro
R4278 T15160 T15158 cc and,vitro
R4279 T15029 T15031 compound type,protein
R4280 T15030 T15029 punct -,type
R4281 T15031 T15027 pobj protein,of
R4282 T15161 T15162 advmod in,vivo
R4283 T15162 T15158 conj vivo,vitro
R4284 T15032 T15023 punct ", ",localizes
R4285 T15163 T15105 punct .,show
R4286 T15165 T15166 csubj Immunostaining,shows
R4287 T15033 T15023 nsubj it,localizes
R4288 T15034 T15023 advmod normally,localizes
R4289 T15167 T15168 det the,kidneys
R4290 T15168 T15165 dobj kidneys,Immunostaining
R4291 T15169 T15168 prep of,kidneys
R4292 T15170 T15171 amod homozygous,mice
R4293 T15035 T14990 punct .,demonstrated
R4294 T15171 T15169 pobj mice,of
R4295 T15172 T15173 compound Aqp2F204V,F204V
R4296 T15173 T15171 compound F204V,mice
R4297 T15037 T15038 det The,mechanism
R4298 T15174 T15173 punct /,F204V
R4299 T15175 T15176 mark that,mediate
R4300 T15176 T15166 ccomp mediate,shows
R4301 T15177 T15178 det the,cells
R4302 T15038 T15040 nsubj mechanism,seems
R4303 T15178 T15176 nsubj cells,mediate
R4304 T15179 T15180 npadvmod mutant,expressing
R4305 T15039 T15038 amod same,mechanism
R4306 T15180 T15178 amod expressing,cells
R4307 T15181 T15180 punct -,expressing
R4308 T15182 T15183 amod collecting,duct
R4309 T15183 T15178 compound duct,cells
R4310 T15184 T15176 aux can,mediate
R4311 T15185 T15176 neg not,mediate
R4312 T15186 T15187 compound water,reabsorption
R4313 T15041 T15042 aux to,apply
R4314 T15187 T15176 dobj reabsorption,mediate
R4315 T15188 T15176 punct ", ",mediate
R4316 T15189 T15190 mark because,fails
R4317 T15190 T15176 advcl fails,mediate
R4318 T15191 T15190 nsubj it,fails
R4319 T15042 T15040 xcomp apply,seems
R4320 T15192 T15193 aux to,insert
R4321 T15193 T15190 xcomp insert,fails
R4322 T15043 T15044 advmod in,vivo
R4323 T15194 T15193 prep into,insert
R4324 T15195 T15196 det the,membrane
R4325 T15196 T15194 pobj membrane,into
R4326 T15044 T15042 advmod vivo,apply
R4327 T15197 T15196 amod apical,membrane
R4328 T15198 T15196 compound plasma,membrane
R4329 T15199 T15193 prep in,insert
R4330 T15045 T15042 prep with,apply
R4331 T15200 T15199 pobj response,in
R4332 T15046 T15047 nmod Aqp2F204V,mice
R4333 T15047 T15045 pobj mice,with
R4334 T15048 T15046 punct /,Aqp2F204V
R4335 T15201 T15200 prep to,response
R4336 T15049 T15046 punct +,Aqp2F204V
R4337 T15050 T15040 punct .,seems
R4338 T15202 T15201 pobj dDAVP,to
R4339 T15203 T15166 punct .,shows
R4340 T15052 T15053 prep In,interact
R4341 T15205 T15206 nsubj This,is
R4342 T15054 T15052 pobj support,In
R4343 T15207 T15208 det this,proof
R4344 T15208 T15206 attr proof,is
R4345 T15209 T15208 amod first,proof
R4346 T15055 T15054 prep of,support
R4347 T15210 T15211 advmod in,vivo
R4348 T15211 T15208 amod vivo,proof
R4349 T15212 T15208 prep of,proof
R4350 T15267 T15268 advmod Thus,proposed
R4351 T15213 T15214 det a,hypothesis
R4352 T15214 T15212 pobj hypothesis,of
R4353 T15215 T15216 amod long,standing
R4354 T15216 T15214 amod standing,hypothesis
R4355 T15217 T15216 punct -,standing
R4356 T15269 T15268 punct ", ",proposed
R4357 T15218 T15219 dep that,comes
R4358 T15219 T15214 relcl comes,hypothesis
R4359 T15220 T15219 prep from,comes
R4360 T15221 T15222 advmod in,vitro
R4361 T15222 T15223 amod vitro,studies
R4362 T15223 T15220 pobj studies,from
R4363 T15224 T15223 prep with,studies
R4364 T15270 T15268 nsubjpass misfolding,proposed
R4365 T15225 T15226 amod recessive,mutations
R4366 T15226 T15224 pobj mutations,with
R4367 T15227 T15226 compound Aqp2,mutations
R4368 T15228 T15206 punct .,is
R4369 T15271 T15270 punct ", ",misfolding
R4370 T15230 T15231 nsubj Transfection,shows
R4371 T15272 T15270 conj retention,misfolding
R4372 T15232 T15230 prep into,Transfection
R4373 T15233 T15234 compound MDCK,cells
R4374 T15273 T15272 prep in,retention
R4375 T15234 T15232 pobj cells,into
R4376 T15235 T15230 prep of,Transfection
R4377 T15236 T15235 pobj any,of
R4378 T15274 T15275 det the,ER
R4379 T15237 T15236 prep of,any
R4380 T15238 T15239 amod several,mutations
R4381 T15239 T15237 pobj mutations,of
R4382 T15275 T15273 pobj ER,in
R4383 T15240 T15239 compound Aqp2,mutations
R4384 T15241 T15239 acl corresponding,mutations
R4385 T15276 T15272 punct ", ",retention
R4386 T15242 T15241 prep to,corresponding
R4387 T15243 T15244 amod recessive,alleles
R4388 T15244 T15242 pobj alleles,to
R4389 T15245 T15244 amod human,alleles
R4390 T15277 T15272 cc and,retention
R4391 T15246 T15247 amod abnormal,localization
R4392 T15247 T15231 dobj localization,shows
R4393 T15278 T15272 conj failure,retention
R4394 T15248 T15247 amod subcellular,localization
R4395 T15249 T15250 punct [,25
R4396 T15279 T15280 aux to,translocate
R4397 T15250 T15247 parataxis 25,localization
R4398 T15251 T15250 punct ],25
R4399 T15252 T15247 punct ", ",localization
R4400 T15280 T15278 acl translocate,failure
R4401 T15253 T15254 punct [,27
R4402 T15254 T15247 parataxis 27,localization
R4403 T15255 T15254 punct ],27
R4404 T15281 T15280 prep in,translocate
R4405 T15256 T15247 cc and,localization
R4406 T15257 T15247 conj failure,localization
R4407 T15258 T15259 aux to,translocate
R4408 T15282 T15281 pobj response,in
R4409 T15259 T15257 acl translocate,failure
R4410 T15260 T15259 advmod appropriately,translocate
R4411 T15261 T15259 prep to,translocate
R4412 T15283 T15282 prep to,response
R4413 T15262 T15263 det the,membrane
R4414 T15263 T15261 pobj membrane,to
R4415 T15264 T15263 compound plasma,membrane
R4416 T15265 T15231 punct .,shows
R4417 T15284 T15283 pobj dDAVP,to
R4418 T15285 T15268 auxpass were,proposed
R4419 T15286 T15268 prep as,proposed
R4420 T15287 T15288 det the,mechanism
R4421 T15288 T15286 pobj mechanism,as
R4422 T15289 T15288 prep for,mechanism
R4423 T15290 T15291 amod autosomal,NDI
R4424 T15291 T15289 pobj NDI,for
R4425 T15292 T15291 amod recessive,NDI
R4426 T15293 T15268 punct .,proposed
R4427 T15295 T15296 advmod Here,prove
R4428 T15297 T15296 nsubj we,prove
R4429 T15298 T15296 preconj not,prove
R4430 T15299 T15298 advmod only,not
R4431 T15300 T15301 det this,hypothesis
R4432 T15301 T15296 dobj hypothesis,prove
R4433 T15302 T15296 cc but,prove
R4434 T15303 T15302 advmod also,but
R4435 T15304 T15296 conj establish,prove
R4436 T15305 T15306 det a,model
R4437 T15306 T15304 dobj model,establish
R4438 T15307 T15306 amod useful,model
R4439 T15308 T15306 prep for,model
R4440 T15309 T15310 amod human,NDI
R4441 T15310 T15308 pobj NDI,for
R4442 T15311 T15296 punct .,prove
R4443 T15313 T15314 det This,model
R4444 T15314 T15316 nsubj model,provides
R4445 T15315 T15314 compound mouse,model
R4446 T15317 T15314 prep of,model
R4447 T15318 T15317 pobj NDI,of
R4448 T15319 T15314 prep based,model
R4449 T15320 T15319 prep on,based
R4450 T15321 T15322 det an,allele
R4451 T15322 T15320 pobj allele,on
R4452 T15323 T15322 compound Aqp2,allele
R4453 T15324 T15325 dep that,rescued
R4454 T15325 T15322 relcl rescued,allele
R4455 T15326 T15325 aux can,rescued
R4456 T15327 T15325 auxpass be,rescued
R4457 T15328 T15329 det the,opportunity
R4458 T15329 T15316 dobj opportunity,provides
R4459 T15330 T15331 aux to,test
R4460 T15331 T15329 acl test,opportunity
R4461 T15332 T15331 dobj therapies,test
R4462 T15333 T15332 punct ", ",therapies
R4463 T15334 T15332 prep including,therapies
R4464 T15335 T15336 compound gene,therapy
R4465 T15336 T15334 pobj therapy,including
R4466 T15337 T15332 punct ", ",therapies
R4467 T15338 T15339 dep that,promote
R4468 T15339 T15332 relcl promote,therapies
R4469 T15340 T15339 aux may,promote
R4470 T15341 T15342 amod proper,localization
R4471 T15342 T15339 dobj localization,promote
R4472 T15343 T15342 amod subcellular,localization
R4473 T15344 T15316 punct .,provides

2_test

Id Subject Object Predicate Lexical cue
16121255-11104789-85587801 375-377 11104789 denotes 15
16121255-11035038-85587802 795-797 11035038 denotes 18
16121255-11035038-85587803 1175-1177 11035038 denotes 18
16121255-12470740-85587804 3376-3378 12470740 denotes 26
16121255-14662748-85587805 4776-4777 14662748 denotes 6
16121255-15509592-85587806 5457-5459 15509592 denotes 25
16121255-9593782-85587807 5463-5465 9593782 denotes 27
T41480 375-377 11104789 denotes 15
T81534 795-797 11035038 denotes 18
T96150 1175-1177 11035038 denotes 18
T95394 3376-3378 12470740 denotes 26
T49784 4776-4777 14662748 denotes 6
T81260 5457-5459 15509592 denotes 25
T11716 5463-5465 9593782 denotes 27