PMC:1189073 / 14484-15138 JSONTXT

{"project":"craft-ca-core-ex-dev","denotations":[{"id":"T8613","span":{"begin":505,"end":511},"obj":"NCBITaxon:9606"},{"id":"T8614","span":{"begin":523,"end":530},"obj":"SO_EXT:0001023"},{"id":"T8615","span":{"begin":534,"end":538},"obj":"PR_EXT:000004182"},{"id":"T8616","span":{"begin":596,"end":607},"obj":"GO_EXT:biological_movement_or_translocation_process"},{"id":"T8617","span":{"begin":615,"end":621},"obj":"GO:0045177"},{"id":"T8618","span":{"begin":622,"end":626},"obj":"CL_GO_EXT:cell"},{"id":"T8619","span":{"begin":622,"end":634},"obj":"GO:0009986"},{"id":"T8620","span":{"begin":638,"end":646},"obj":"GO_EXT:reaction_or_response"},{"id":"T8621","span":{"begin":650,"end":653},"obj":"CHEBI_PR_EXT:arginine_vasopressin"}],"text":"\nFigure 3 Immunoblot Analyses of AQP2 from Mouse Kidneys\n(A) Western blot analyses of total kidney membranes from littermate mice. An intermediate form of AQP2 at 31 kDa was identified in kidney membranes from a mutant mouse (Mut) and partially in a heterozygous mouse (Het).\n(B) Total kidney membranes were subjected to endoglycosidase H treatment (Endo H) prior to Western blotting. High-mannose (h.m.) glycosylated proteins that have not exited the ER are sensitive to endoglycosidase H digestion. In humans, recessive alleles of Aqp2 are postulated to cause NDI because they do not properly translocate to the apical cell surface in response to AVP."}

{"project":"craft-ca-core-dev","denotations":[{"id":"T8225","span":{"begin":505,"end":511},"obj":"NCBITaxon:9606"},{"id":"T8226","span":{"begin":523,"end":530},"obj":"SO:0001023"},{"id":"T8227","span":{"begin":534,"end":538},"obj":"PR:000004182"},{"id":"T8228","span":{"begin":615,"end":621},"obj":"GO:0045177"},{"id":"T8229","span":{"begin":622,"end":634},"obj":"GO:0009986"}],"text":"\nFigure 3 Immunoblot Analyses of AQP2 from Mouse Kidneys\n(A) Western blot analyses of total kidney membranes from littermate mice. An intermediate form of AQP2 at 31 kDa was identified in kidney membranes from a mutant mouse (Mut) and partially in a heterozygous mouse (Het).\n(B) Total kidney membranes were subjected to endoglycosidase H treatment (Endo H) prior to Western blotting. High-mannose (h.m.) glycosylated proteins that have not exited the ER are sensitive to endoglycosidase H digestion. In humans, recessive alleles of Aqp2 are postulated to cause NDI because they do not properly translocate to the apical cell surface in response to AVP."}

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