> top > docs > PMC:1189073 > annotations

PMC:1189073 JSONTXT

Annnotations TAB JSON ListView MergeView

craft-ca-core-ex-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T530 22-26 NCBITaxon:10088 denotes Mice
T531 34-42 SO_EXT:sequence_alteration_entity_or_process denotes Mutation
T532 46-57 PR_EXT:000004182 denotes Aquaporin-2
T533 103-114 GO:0001822 denotes nephrogenic
T534 185-191 UBERON:0002113 denotes kidney
T535 207-212 UBERON:0001088 denotes urine
T536 216-224 GO_EXT:reaction_or_response denotes response
T537 228-239 CHEBI_PR_EXT:vasopressin denotes vasopressin
T538 241-246 NCBITaxon:9606 denotes Human
T539 261-272 GO:0001822 denotes nephrogenic
T540 318-327 SO_EXT:sequence_alteration_entity_or_process denotes mutations
T541 335-346 CHEBI_PR_EXT:vasopressin denotes vasopressin
T542 335-355 GO_EXT:0005000 denotes vasopressin receptor
T543 335-357 PR_EXT:000001683 denotes vasopressin receptor 2
T544 359-364 PR_EXT:000001683 denotes Avpr2
T545 366-370 SO_EXT:0000704 denotes gene
T546 378-389 CHEBI_PR_EXT:vasopressin denotes vasopressin
T547 400-405 CHEBI:15377 denotes water
T548 400-413 GO_EXT:0015250 denotes water channel
T549 414-425 PR_EXT:000004182 denotes aquaporin-2
T550 427-431 PR_EXT:000004182 denotes Aqp2
T551 433-437 SO_EXT:0000704 denotes gene
T552 516-522 NCBITaxon:33208 denotes animal
T553 546-553 SO_EXT:0000704 denotes genetic
T554 567-583 CHEBI:23995 denotes ethylnitrosourea
T555 596-600 NCBITaxon:10088 denotes mice
T556 657-662 NCBITaxon:10088 denotes mouse
T557 691-699 SO_EXT:sequence_alteration_entity_or_process denotes mutation
T558 707-711 PR_EXT:000004182 denotes Aqp2
T559 712-716 SO_EXT:0000704 denotes gene
T560 746-752 NCBITaxon:39107 denotes murine
T561 772-776 NCBITaxon:10088 denotes mice
T562 788-797 UBERON:0000113 denotes adulthood
T563 832-837 NCBITaxon:9606 denotes human
T564 884-889 UBERON:0002113 denotes renal
T565 884-894 CL:1000497 denotes renal cell
T566 890-894 CL_GO_EXT:cell denotes cell
T567 919-923 PR_EXT:000004182 denotes Aqp2
T568 924-933 SO_EXT:sequence_alteration_entity_or_process denotes mutations
T569 953-964 GO_EXT:biological_routing_or_transport denotes trafficking
T570 972-978 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T571 979-984 CHEBI:15377 denotes water
T572 979-989 GO_EXT:0015250 denotes water pore
T573 1003-1010 NCBITaxon:33208 denotes animals
T574 1064-1068 PR_EXT:000004182 denotes AQP2
T575 1069-1082 GO_EXT:biological_translocation_entity_or_process denotes translocation
T576 1090-1099 CHEBI_EXT:polyatomic_entity_or_group denotes molecular
T577 1110-1121 GO:0001822 denotes nephrogenic
T578 1155-1163 NCBITaxon:1 denotes organism
T579 1187-1192 UBERON:0002113 denotes renal
T580 1187-1197 CL:1000497 denotes renal cell
T581 1193-1197 CL_GO_EXT:cell denotes cell
T582 1220-1227 SO_EXT:sequence_alteration_process denotes mutated
T583 1228-1235 CHEBI_PR_EXT:protein denotes protein
T584 1237-1241 PR_EXT:000004182 denotes AQP2
T585 1268-1289 GO:0005783 denotes endoplasmic reticulum
T586 1313-1325 GO_PATO_EXT:biological_localization_process_or_quality denotes localization
T587 1333-1340 SO_EXT:sequence_rescue_process denotes rescued
T588 1344-1353 SO_EXT:wild_type_entity_or_quality denotes wild-type
T589 1354-1361 CHEBI_PR_EXT:protein denotes protein
T590 1374-1379 NCBITaxon:10088 denotes mouse
T591 1447-1462 CHEBI_EXT:52217 denotes pharmacological
T592 1467-1471 SO_EXT:0000704 denotes gene
T2300 3091-3102 GO:0001822 denotes Nephrogenic
T2301 3168-3177 GO:0060073 denotes urination
T2302 3227-3235 CHEBI_EXT:diuretic_agent_or_process denotes diuretic
T2303 3236-3243 CHEBI_GO_EXT:hormone denotes hormone
T2304 3244-3252 CHEBI_SO_EXT:arginine denotes arginine
T2305 3244-3264 CHEBI_PR_EXT:arginine_vasopressin denotes arginine vasopressin
T2306 3266-3269 CHEBI_PR_EXT:arginine_vasopressin denotes AVP
T2307 3307-3308 GO:0000805 denotes X
T2308 3336-3344 SO_EXT:sequence_alteration_entity_or_process denotes mutation
T2309 3352-3357 PR_EXT:000001683 denotes Avpr2
T2310 3358-3362 SO_EXT:0000704 denotes gene
T2311 3371-3380 GO:0030849 denotes autosomal
T2312 3388-3396 SO_EXT:sequence_alteration_entity_or_process denotes mutation
T2313 3404-3408 PR_EXT:000004182 denotes Aqp2
T2314 3409-3413 SO_EXT:0000704 denotes gene
T2315 3419-3430 PR_EXT:000004182 denotes Aquaporin-2
T2316 3432-3436 PR_EXT:000004182 denotes AQP2
T2317 3443-3447 GO_EXT:0015267 denotes pore
T2318 3456-3463 CHEBI_PR_EXT:protein denotes protein
T2319 3489-3494 CHEBI:15377 denotes water
T2320 3489-3503 GO_EXT:0015250 denotes water channels
T2321 3519-3528 GO:0010467 denotes expressed
T2322 3532-3547 UBERON:0001232 denotes collecting-duct
T2323 3532-3563 CL:1001431 denotes collecting-duct principal cells
T2324 3558-3563 CL_GO_EXT:cell denotes cells
T2325 3571-3577 UBERON:0002113 denotes kidney
T2326 3599-3607 CHEBI_PR_EXT:protein denotes proteins
T2327 3619-3635 GO:0006833 denotes passage of water
T2328 3619-3626 _FRAGMENT denotes passage
T2329 3636-3643 _FRAGMENT denotes through
T2330 3655-3663 GO:0055085 denotes membrane
T2331 3630-3635 CHEBI:15377 denotes water
T2332 3648-3663 GO:0005886 denotes plasma membrane
T2333 3665-3667 GO:0005886 denotes PM
T2334 3672-3677 CL_GO_EXT:cell denotes cells
T2335 3747-3752 CHEBI:15377 denotes water
T2336 3747-3765 _FRAGMENT denotes water reabsorption
T2337 3777-3779 _FRAGMENT denotes in
T2338 3784-3790 GO:0070295 denotes kidney
T2339 3771-3776 UBERON:0001088 denotes urine
T2340 3784-3790 UBERON:0002113 denotes kidney
T2341 3867-3878 GO:0051262 denotes tetramerize
T2342 3911-3926 GO:0005886 denotes plasma membrane
T2343 3952-3960 CHEBI_PR_EXT:protein denotes proteins
T2344 3988-3996 GO_EXT:biological_routing_or_transport denotes targeted
T2345 4009-4019 _FRAGMENT denotes regions of
T2346 4024-4026 GO:0044459 denotes PM
T2347 4041-4045 PR_EXT:000004182 denotes AQP2
T2427 5904-5908 PR_EXT:000004182 denotes Aqp2
T2348 4049-4055 GO_EXT:biological_routing_or_transport denotes routed
T2349 4063-4087 GO:0016324 denotes apical membrane of cells
T2350 4082-4087 CL_GO_EXT:cell denotes cells
T2351 4104-4119 UBERON:0001232 denotes collecting duct
T2352 4147-4151 PR_EXT:000004183 denotes AQP3
T2353 4180-4196 GO:1990794 denotes basolateral face
T2354 4231-4235 PR_EXT:000004182 denotes AQP2
T2355 4276-4291 GO:0005886 denotes plasma membrane
T2356 4321-4328 CHEBI_PR_EXT:protein denotes protein
T2357 4343-4349 GO:0016324 denotes apical
T2358 4350-4363 GO:0005622 denotes intracellular
T2359 4355-4363 CL_GO_EXT:cell denotes cellular
T2360 4364-4372 GO:0031982 denotes vesicles
T2361 4410-4415 CHEBI:15377 denotes water
T2362 4426-4430 PR_EXT:000004182 denotes AQP2
T2363 4431-4443 GO_EXT:biological_movement_or_translocation_process denotes translocates
T2364 4451-4466 GO:0016324 denotes apical membrane
T2365 4479-4484 CHEBI:15377 denotes water
T2366 4532-4535 CHEBI_PR_EXT:arginine_vasopressin denotes AVP
T2367 4536-4541 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes binds
T2368 4546-4554 GO_EXT:0004872 denotes receptor
T2369 4556-4561 PR_EXT:000001683 denotes AVPR2
T2370 4570-4589 _FRAGMENT denotes basolateral face of
T2371 4610-4615 GO:1990794 denotes cells
T2372 4594-4609 UBERON:0001232 denotes collecting duct
T2373 4594-4615 CL:1001225 denotes collecting duct cells
T2374 4638-4651 GO:0005622 denotes intracellular
T2375 4643-4651 CL_GO_EXT:cell denotes cellular
T2376 4652-4656 CHEBI:17489 denotes cAMP
T2377 4682-4697 GO_PATO_EXT:phosphorylation_process denotes phosphorylation
T2378 4701-4705 PR_EXT:000004182 denotes AQP2
T2379 4709-4715 CHEBI_SO_EXT:serine denotes serine
T2380 4723-4727 CHEBI:17489 denotes cAMP
T2381 4723-4752 GO_EXT:0004691 denotes cAMP-dependent protein kinase
T2382 4738-4745 CHEBI_PR_EXT:protein denotes protein
T2383 4787-4802 GO:0005886 denotes plasma membrane
T2384 4822-4826 PR_EXT:000004182 denotes AQP2
T2385 4897-4901 PR_EXT:000004182 denotes Aqp2
T2386 4902-4911 SO_EXT:sequence_alteration_entity_or_process denotes mutations
T2387 4939-4945 NCBITaxon:9606 denotes humans
T2388 4964-4968 PR_EXT:000004182 denotes Aqp2
T2389 4969-4978 SO_EXT:sequence_alteration_entity_or_process denotes mutations
T2390 5026-5035 GO_PATO_EXT:biological_localization_process_or_quality denotes localized
T2392 5070-5075 CHEBI:15377 denotes water
T2393 5070-5080 GO_EXT:0015250 denotes water pore
T2394 5086-5094 GO_EXT:reaction_or_response denotes responds
T2395 5124-5128 CHEBI:17489 denotes cAMP
T2396 5159-5168 SO_EXT:sequence_alteration_entity_or_process denotes mutations
T2397 5205-5210 GO_EXT:biological_routing_or_transport denotes route
T2398 5220-5226 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T2399 5235-5244 SO_EXT:wild_type_entity_or_quality denotes wild-type
T2400 5245-5252 CHEBI_PR_EXT:protein denotes protein
T2401 5260-5280 GO:0016323 denotes basolateral membrane
T2402 5297-5302 NCBITaxon:10088 denotes mouse
T2403 5391-5396 NCBITaxon:9606 denotes human
T2404 5402-5406 NCBITaxon:10088 denotes mice
T2405 5432-5441 SO_EXT:sequence_alteration_entity_or_process denotes mutations
T2406 5445-5449 PR_EXT:000004182 denotes Aqp2
T2407 5454-5459 PR_EXT:000001683 denotes Avpr2
T2408 5499-5504 NCBITaxon:10088 denotes mouse
T2409 5527-5535 SO_EXT:sequence_alteration_entity_or_process denotes mutation
T2410 5543-5547 PR_EXT:000004182 denotes Aqp2
T2411 5548-5552 SO_EXT:0000704 denotes gene
T2412 5579-5585 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T2413 5586-5590 NCBITaxon:10088 denotes mice
T2414 5591-5595 GO:0016265 denotes died
T2415 5613-5618 GO:0007567 denotes birth
T2416 5635-5640 PR_EXT:000001683 denotes AVPR2
T2417 5651-5655 PATO_UBERON_EXT:male_or_bearer_of_maleness denotes male
T2418 5656-5660 NCBITaxon_UBERON_EXT:pup denotes pups
T2419 5666-5669 GO:0016265 denotes die
T2420 5698-5703 GO:0007567 denotes birth
T2421 5744-5749 NCBITaxon:10088 denotes mouse
T2422 5776-5784 NCBITaxon:1 denotes organism
T2423 5800-5805 CHEBI:15377 denotes water
T2424 5800-5817 GO:0030104 denotes water homeostasis
T2425 5872-5879 SO_EXT:0000704 denotes genetic
T2426 5890-5895 NCBITaxon:10088 denotes mouse
T2428 5909-5917 SO_EXT:sequence_alteration_entity_or_process denotes mutation
T2429 5985-5991 NCBITaxon:39107 denotes murine
T2430 6011-6022 GO:0001822 denotes nephrogenic
T2431 6055-6063 SO_EXT:sequence_alteration_entity_or_process denotes mutation
T2432 6071-6075 PR_EXT:000004182 denotes Aqp2
T2433 6076-6080 SO_EXT:0000704 denotes gene
T2434 6087-6093 SO_EXT:0001023 denotes allele
T2435 6097-6101 PR_EXT:000004182 denotes Aqp2
T2436 6131-6136 NCBITaxon:10088 denotes mouse
T2437 6184-6188 UBERON:0000104 denotes life
T2438 6190-6199 CHEBI_EXT:polyatomic_entity_or_group denotes Molecular
T2439 6224-6230 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T2440 6231-6235 PR_EXT:000004182 denotes AQP2
T2441 6255-6266 GO_UBERON_EXT:cellular_component_or_cell_part denotes subcellular
T2442 6258-6266 CL_GO_EXT:cell denotes cellular
T2443 6267-6279 GO_PATO_EXT:biological_localization_process_or_quality denotes localization
T2444 6283-6304 UBERON:0001232 denotes renal collecting-duct
T2445 6283-6310 CL:1001225 denotes renal collecting-duct cells
T2446 6305-6310 CL_GO_EXT:cell denotes cells
T2447 6333-6346 GO_EXT:biological_translocation_entity_or_process denotes translocation
T2448 6358-6370 CHEBI:4450 denotes desmopressin
T2449 6375-6382 CHEBI_GO_EXT:agonist denotes agonist
T2450 6386-6389 CHEBI_PR_EXT:arginine_vasopressin denotes AVP
T2451 6430-6436 NCBITaxon:9608 denotes canine
T2452 6437-6443 UBERON:0002113 denotes kidney
T2453 6437-6443 _FRAGMENT denotes kidney
T2454 6451-6455 CL:1000497 denotes cell
T2455 6451-6455 CL_GO_EXT:cell denotes cell
T2456 6477-6498 GO:0005783 denotes endoplasmic reticulum
T2457 6515-6521 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T2458 6522-6529 CHEBI_PR_EXT:protein denotes protein
T2459 6558-6571 GO_EXT:biological_translocation_entity_or_process denotes translocation
T2460 6608-6617 GO:0030849 denotes autosomal
T2461 6661-6665 PR_EXT:000004182 denotes AQP2
T2462 6666-6673 GO_EXT:biological_routing_or_transport denotes routing
T2463 6688-6694 NCBITaxon:40674 denotes mammal
T8418 7258-7271 SO_EXT:coding_sequence denotes coding region
T8419 6718-6725 SO_EXT:0000704 denotes genetic
T8420 6743-6759 CHEBI:23995 denotes ethylnitrosourea
T8421 6761-6764 CHEBI:23995 denotes ENU
T8422 6776-6785 SO_EXT:sequence_alteration_entity_or_process denotes mutations
T8423 6799-6805 NCBITaxon:33208 denotes animal
T8424 6860-6864 UBERON_EXT:body denotes body
T8425 6865-6875 GO:0008152 denotes metabolism
T8426 6906-6910 NCBITaxon:10088 denotes mice
T8427 6916-6924 GO:0060073 denotes urinated
T8428 6929-6934 GO:0042756 denotes drank
T8429 6948-6953 UBERON:0001977 denotes Serum
T8430 6958-6963 UBERON:0001088 denotes urine
T8431 6985-6991 UBERON:0001969 denotes plasma
T8432 6992-6999 CHEBI:17234 denotes glucose
T8433 7036-7043 CHEBI:17234 denotes glucose
T8434 7051-7056 UBERON:0001088 denotes urine
T8435 7149-7153 NCBITaxon:10088 denotes mice
T8436 7174-7179 SO_EXT:0000704 denotes genic
T8437 7181-7190 GO:0030849 denotes autosomal
T8438 7216-7220 PR_EXT:000004182 denotes Aqp2
T8439 7233-7237 SO_EXT:0000704 denotes gene
T8440 7253-7257 PR_EXT:000004182 denotes Aqp2
T8441 7284-7288 NCBITaxon:10088 denotes mice
T8442 7302-7309 CHEBI_EXT:thymine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group denotes thymine
T8443 7302-7320 _FRAGMENT denotes thymine to guanine
T8444 7330-7342 SO_EXT:1000022 denotes transversion
T8445 7313-7320 CHEBI_EXT:guanine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group denotes guanine
T8446 7322-7323 CHEBI_EXT:thymine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group denotes T
T8447 7322-7328 _FRAGMENT denotes T to G
T8448 7327-7328 CHEBI_EXT:guanine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group denotes G
T8449 7388-7394 CHEBI_SO_EXT:valine denotes valine
T8450 7399-7412 CHEBI_SO_EXT:phenylalanine denotes phenylalanine
T8451 7413-7425 SO_EXT:sequence_substitution_entity_or_process denotes substitution
T8452 7429-7439 CHEBI_SO_EXT:amino_acid denotes amino acid
T8453 7451-7458 CHEBI_PR_EXT:protein denotes protein
T8454 8492-8496 PR_EXT:000004182 denotes AQP2
T8455 8511-8519 GO:0016020 denotes membrane
T8456 8520-8525 CHEBI:15377 denotes water
T8457 8520-8533 GO_EXT:0015250 denotes water channel
T8458 8558-8571 GO:0005576 denotes extracellular
T8459 8563-8571 CL_GO_EXT:cell denotes cellular
T8460 8590-8598 GO:0016020 denotes membrane
T8461 8608-8614 SO_EXT:0000417 denotes domain
T8462 8645-8656 CHEBI_SO_EXT:amino_acid denotes amino acids
T8463 8677-8685 GO:0016020 denotes membrane
T8464 8695-8702 SO_EXT:0000417 denotes domains
T8465 8707-8716 SO_EXT:biological_conservation_process_or_quality denotes conserved
T8466 8723-8733 NCBITaxon:7742 denotes vertebrate
T8467 8734-8741 NCBITaxon:species denotes species
T8468 8747-8760 CHEBI_SO_EXT:phenylalanine denotes phenylalanine
T8469 8798-8807 SO_EXT:biological_conservation_process_or_quality denotes conserved
T8470 8836-8846 NCBITaxon:7742 denotes vertebrate
T8471 8847-8851 PR_EXT:000004182 denotes AQP2
T8472 8852-8860 CHEBI_PR_EXT:protein denotes proteins
T8473 8908-8912 PR_EXT:000004182 denotes Aqp2
T8474 8924-8928 NCBITaxon:10088 denotes mice
T8475 8957-8962 UBERON:0001088 denotes urine
T8476 9012-9017 UBERON:0001088 denotes urine
T8477 9045-9049 UBERON_EXT:body denotes body
T8478 9092-9101 SO_EXT:wild_type_entity_or_quality denotes wild-type
T8479 9128-9133 CHEBI:15377 denotes water
T8480 9205-9210 CHEBI:15377 denotes water
T8481 9211-9217 GO:0007631 denotes intake
T8482 9227-9233 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T8483 9234-9238 NCBITaxon:10088 denotes mice
T8484 9272-9277 CHEBI:15377 denotes water
T8485 9278-9284 GO:0007631 denotes intake
T8486 9331-9340 SO_EXT:wild_type_entity_or_quality denotes wild-type
T8487 9379-9386 UBERON:0001088 denotes urinary
T8488 9398-9403 CHEBI:15377 denotes water
T8489 9404-9410 GO:0007631 denotes intake
T8490 9470-9478 SO_EXT:sequence_alteration_entity_or_process denotes mutation
T8491 9489-9496 NCBITaxon:33208 denotes animals
T8492 9570-9575 UBERON:0001088 denotes urine
T8493 9607-9616 SO_EXT:wild_type_entity_or_quality denotes wild-type
T8494 9646-9650 PR_EXT:000004182 denotes Aqp2
T8495 9662-9666 NCBITaxon:10088 denotes mice
T8496 9687-9692 UBERON:0001088 denotes urine
T8497 9712-9717 UBERON:0001088 denotes urine
T8498 9735-9741 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T8499 9742-9746 NCBITaxon:10088 denotes mice
T8500 9798-9807 SO_EXT:wild_type_entity_or_quality denotes wild-type
T8501 9808-9812 NCBITaxon:10088 denotes mice
T8502 9836-9841 UBERON:0001088 denotes urine
T8503 9869-9876 GO:0065007 denotes control
T8504 9884-9896 UBERON:0001898 denotes hypothalamus
T8505 9908-9916 GO_EXT:reaction_or_response denotes response
T8506 9955-9963 GO:0046903 denotes secretes
T8507 9964-9967 CHEBI_PR_EXT:arginine_vasopressin denotes AVP
T8508 9983-9986 CHEBI_PR_EXT:arginine_vasopressin denotes AVP
T8509 9995-10029 CHEBI:4450 denotes 1-deamino-8-D-arginine vasopressin
T8510 10031-10036 CHEBI:4450 denotes dDAVP
T8511 10050-10062 CHEBI:4450 denotes desmopressin
T8512 10077-10084 CHEBI_GO_EXT:agonist denotes agonist
T8513 10088-10093 PR_EXT:000001683 denotes AVPR2
T8514 10116-10125 SO_EXT:wild_type_entity_or_quality denotes wild-type
T8515 10126-10130 NCBITaxon:10088 denotes mice
T8516 10132-10137 CHEBI:4450 denotes dDAVP
T8517 10170-10175 UBERON:0001088 denotes urine
T8518 10263-10269 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T8519 10270-10274 NCBITaxon:10088 denotes mice
T8520 10293-10298 UBERON:0001088 denotes urine
T8521 10396-10403 NCBITaxon:33208 denotes animals
T8522 10445-10450 UBERON:0001088 denotes urine
T8523 10492-10500 GO_EXT:reaction_or_response denotes response
T8525 10523-10531 GO_EXT:reaction_or_response denotes response
T8526 10535-10540 CHEBI:4450 denotes dDAVP
T8527 10581-10587 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T8528 10588-10592 PR_EXT:000004182 denotes AQP2
T8529 10593-10600 GO_EXT:0015267 denotes channel
T8530 10652-10662 NCBITaxon:1 denotes individual
T8531 10698-10703 NCBITaxon:10088 denotes mouse
T8532 10732-10739 GO:0007618 denotes matings
T8533 10752-10759 NCBITaxon:33208 denotes animals
T8534 10828-10837 SO_EXT:wild_type_entity_or_quality denotes wild type
T8535 10857-10863 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T8536 10958-10966 SO_EXT:sequence_alteration_entity_or_process denotes mutation
T8537 10993-10998 UBERON:0001088 denotes urine
T8538 11014-11019 CHEBI:15377 denotes water
T8539 11020-11026 GO:0007631 denotes intake
T8540 11060-11066 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T8541 11067-11071 NCBITaxon:10088 denotes mice
T8542 11088-11095 UBERON:0002113 denotes kidneys
T8543 11130-11137 NCBITaxon:33208 denotes animals
T8544 11187-11193 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T8545 11194-11198 NCBITaxon:10088 denotes mice
T8546 11221-11229 UBERON:0000104 denotes lifespan
T8547 11239-11245 NCBITaxon:33208 denotes animal
T8548 11292-11299 NCBITaxon:33208 denotes animals
T8549 12159-12163 PR_EXT:000004182 denotes Aqp2
T8550 12175-12179 NCBITaxon:10088 denotes mice
T8551 12332-12339 UBERON:0002113 denotes kidneys
T8552 12347-12351 PR_EXT:000004182 denotes Aqp2
T8553 12363-12367 NCBITaxon:10088 denotes mice
T8554 12427-12431 NCBITaxon:10088 denotes mice
T8555 12516-12520 PR_EXT:000004177 denotes Aqp1
T8556 12521-12525 PR_EXT:000004183 denotes Aqp3
T8557 12536-12540 NCBITaxon:10088 denotes mice
T8558 12582-12586 PR_EXT:000004182 denotes Aqp2
T8559 12598-12602 NCBITaxon:10088 denotes mice
T8560 12648-12652 PR_EXT:000004182 denotes Aqp2
T8561 12664-12668 NCBITaxon:10088 denotes mice
T8562 12707-12719 UBERON:0001224 denotes renal pelvis
T8563 12749-12755 UBERON:0000056 denotes ureter
T8564 12795-12813 UBERON:0006660 denotes muscularis propria
T8565 12902-12912 UBERON:0000365 denotes urothelium
T8566 12923-12946 UBERON:0005910 denotes transitional epithelium
T8567 12998-13004 UBERON:0002113 denotes kidney
T8568 13022-13035 UBERON:0002015 denotes renal capsule
T8569 13039-13051 UBERON:0001224 denotes renal pelvis
T8570 13094-13103 UBERON:0000074 denotes glomeruli
T8571 13108-13116 _FRAGMENT denotes proximal
T8572 13124-13131 UBERON:0004134 denotes tubules
T8573 13117-13131 UBERON:0004135 denotes distal tubules
T8574 13241-13245 PR_EXT:000004182 denotes AQP2
T8575 13285-13298 GO_MOP_EXT:glycosylation denotes glycosylation
T8576 13355-13367 GO_MOP_EXT:glycosylation denotes glycosylated
T8577 13368-13375 CHEBI_PR_EXT:protein denotes protein
T8578 13416-13428 GO_MOP_EXT:glycosylation denotes glycosylated
T8579 13429-13436 CHEBI_PR_EXT:protein denotes protein
T8580 13542-13549 CHEBI:37684 denotes mannose
T8581 13550-13563 GO_MOP_EXT:glycosylation denotes glycosylation
T8582 13567-13571 PR_EXT:000004182 denotes AQP2
T8583 13601-13609 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes labeling
T8584 13654-13661 UBERON:0002113 denotes kidneys
T8585 13665-13674 SO_EXT:wild_type_entity_or_quality denotes wild-type
T8586 13675-13682 NCBITaxon:33208 denotes animals
T8587 13684-13688 PR_EXT:000004182 denotes AQP2
T8588 13694-13700 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T8589 13701-13708 NCBITaxon:33208 denotes animals
T8590 13738-13747 CHEBI_EXT:polyatomic_entity_or_group denotes molecular
T8591 13784-13793 CHEBI_EXT:polyatomic_entity_or_group denotes molecular
T8592 13840-13849 CHEBI_EXT:polyatomic_entity_or_group denotes molecular
T8593 13888-13895 NCBITaxon:33208 denotes animals
T8594 13964-13976 GO_MOP_EXT:glycosylation denotes glycosylated
T8595 14052-14059 MOP:0000780 denotes cleaves
T8596 14060-14067 CHEBI:37684 denotes mannose
T8597 14073-14085 CHEBI:16646 denotes carbohydrate
T8598 14095-14102 CHEBI_PR_EXT:protein denotes protein
T8599 14137-14141 PR_EXT:000004182 denotes AQP2
T8600 14147-14154 UBERON:0002113 denotes kidneys
T8601 14158-14167 SO_EXT:wild_type_entity_or_quality denotes wild-type
T8602 14187-14193 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T8603 14194-14201 NCBITaxon:33208 denotes animals
T8604 14241-14250 CHEBI_EXT:polyatomic_entity_or_group denotes molecular
T8605 14304-14316 GO_MOP_EXT:glycosylation denotes glycosylated
T8606 14317-14325 CHEBI_PR_EXT:protein denotes proteins
T8607 14341-14345 PR_EXT:000004182 denotes Aqp2
T8608 14357-14361 NCBITaxon:10088 denotes mice
T8609 14408-14412 PR_EXT:000004182 denotes Aqp2
T8610 14424-14428 NCBITaxon:10088 denotes mice
T8611 14466-14474 GO_EXT:reaction_or_response denotes response
T8612 14478-14483 CHEBI:4450 denotes dDAVP
T8613 14989-14995 NCBITaxon:9606 denotes humans
T8614 15007-15014 SO_EXT:0001023 denotes alleles
T8615 15018-15022 PR_EXT:000004182 denotes Aqp2
T8616 15080-15091 GO_EXT:biological_movement_or_translocation_process denotes translocate
T8617 15099-15105 GO:0045177 denotes apical
T8618 15106-15110 CL_GO_EXT:cell denotes cell
T8619 15106-15118 GO:0009986 denotes cell surface
T8620 15122-15130 GO_EXT:reaction_or_response denotes response
T8621 15134-15137 CHEBI_PR_EXT:arginine_vasopressin denotes AVP
T8622 15198-15204 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T8623 15205-15209 PR_EXT:000004182 denotes Aqp2
T8624 15210-15215 SO_EXT:cDNA denotes cDNAs
T8625 15233-15238 NCBITaxon:9606 denotes human
T8626 15247-15256 SO_EXT:sequence_alteration_entity_or_process denotes mutations
T8627 15261-15272 GO:0009294 denotes transfected
T8628 15278-15284 UBERON:0002113 denotes kidney
T8629 15278-15289 CL:1000497 denotes kidney cell
T8630 15285-15289 CL_GO_EXT:cell denotes cell
T8631 15324-15331 SO_EXT:0001023 denotes alleles
T8632 15379-15387 GO_PATO_EXT:biological_localization_process_or_quality denotes localize
T8633 15391-15394 CHEBI_PR_EXT:arginine_vasopressin denotes AVP
T8634 15406-15414 GO:0031982 denotes vesicles
T8635 15448-15469 GO:0005783 denotes endoplasmic reticulum
T8636 15471-15473 GO:0005783 denotes ER
T8637 15480-15485 NCBITaxon:10088 denotes mouse
T8638 15565-15571 NCBITaxon:33208 denotes animal
T8639 15626-15630 PR_EXT:000004182 denotes AQP2
T8640 15654-15663 GO_PATO_EXT:biological_localization_process_or_quality denotes localized
T8641 15674-15680 GO:0016324 denotes apical
T8642 15681-15690 _FRAGMENT denotes region of
T8643 15707-15712 GO:0044464 denotes cells
T8644 15691-15706 UBERON:0001232 denotes collecting duct
T8645 15691-15712 CL:1001225 denotes collecting duct cells
T8646 15716-15723 UBERON:0002113 denotes kidneys
T8647 15727-15736 SO_EXT:wild_type_entity_or_quality denotes wild-type
T8648 15737-15741 NCBITaxon:10088 denotes mice
T8649 15765-15770 CHEBI:4450 denotes dDAVP
T8650 15772-15776 PR_EXT:000004182 denotes AQP2
T8651 15777-15789 GO_EXT:biological_movement_or_translocation_process denotes translocated
T8652 15805-15809 CL_GO_EXT:cell denotes cell
T8653 15805-15817 GO:0009986 denotes cell surface
T8654 15846-15853 UBERON:0002113 denotes kidneys
T8655 15865-15871 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T8656 15872-15879 NCBITaxon:33208 denotes animals
T8657 15890-15894 PR_EXT:000004182 denotes AQP2
T8658 15935-15939 CL_GO_EXT:cell denotes cell
T8659 15989-15993 PR_EXT:000004183 denotes AQP3
T8660 16016-16025 GO_PATO_EXT:biological_localization_process_or_quality denotes localized
T8661 16033-16044 GO:1990794 denotes basolateral
T8662 16045-16052 GO:0009986 denotes surface
T8663 16077-16082 CHEBI:4450 denotes dDAVP
T8664 16096-16100 PR_EXT:000004182 denotes AQP2
T8665 16117-16128 GO_EXT:biological_movement_or_translocation_process denotes translocate
T8666 16136-16140 CL_GO_EXT:cell denotes cell
T8667 16136-16148 GO:0009986 denotes cell surface
T8668 16230-16237 UBERON:0002113 denotes kidneys
T8669 16261-16265 NCBITaxon:10088 denotes mice
T8670 16282-16291 SO_EXT:wild_type_entity_or_quality denotes wild-type
T8671 16295-16301 SO_EXT:sequence_altered_entity denotes mutant
T8672 16319-16324 CHEBI:4450 denotes dDAVP
T8673 18070-18083 GO_EXT:biological_translocation_entity_or_process denotes translocation
T8674 18087-18091 PR_EXT:000004182 denotes AQP2
T8675 18112-18124 GO:0009294 denotes transfection
T8676 18133-18138 CL_GO_EXT:cell denotes cells
T8677 18147-18151 CL_GO_EXT:cell denotes cell
T8678 18158-18168 GO:0010467 denotes expressing
T8679 18169-18174 NCBITaxon:10088 denotes mouse
T8680 18175-18184 SO_EXT:wild_type_entity_or_quality denotes wild-type
T8681 18185-18189 PR_EXT:000004182 denotes AQP2
T8682 18194-18198 PR_EXT:000004182 denotes AQP2
T8683 18238-18245 CHEBI_PR_EXT:protein denotes protein
T8684 18267-18271 CL_GO_EXT:cell denotes cell
T8685 18295-18300 CL_GO_EXT:cell denotes cells
T8686 18318-18331 GO_MOP_EXT:glycosylation denotes glycosylation
T8687 18347-18353 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T8688 18354-18358 NCBITaxon:10088 denotes mice
T8689 18383-18392 SO_EXT:wild_type_entity_or_quality denotes wild-type
T8690 18393-18400 CHEBI_PR_EXT:protein denotes protein
T8691 18445-18450 CL_GO_EXT:cell denotes Cells
T8692 18451-18461 GO:0010467 denotes expressing
T8693 18462-18466 PR_EXT:000004182 denotes AQP2
T8694 18529-18541 GO_MOP_EXT:glycosylation denotes glycosylated
T8695 18558-18569 GO:0009294 denotes transfected
T8696 18589-18594 CL_GO_EXT:cell denotes cells
T8697 18596-18605 SO_EXT:wild_type_entity_or_quality denotes wild-type
T8698 18606-18610 PR_EXT:000004182 denotes AQP2
T8699 18688-18694 GO:0016324 denotes apical
T8700 18761-18770 GO:0031982 denotes vesicular
T8701 18793-18797 PR_EXT:000004182 denotes AQP2
T8702 18855-18862 GO:0005634 denotes nuclear
T8703 18920-18929 CHEBI:42471 denotes forskolin
T8704 18933-18937 CHEBI:17489 denotes cAMP
T8705 18933-18962 GO_EXT:0004691 denotes cAMP-dependent protein kinase
T8706 18948-18955 CHEBI_PR_EXT:protein denotes protein
T8707 18948-18972 GO_EXT:0030295 denotes protein kinase activator
T8708 18974-18983 SO_EXT:wild_type_entity_or_quality denotes wild-type
T8709 18984-18988 PR_EXT:000004182 denotes AQP2
T8710 18989-19001 GO_EXT:biological_movement_or_translocation_process denotes translocated
T8711 19009-19015 GO:0045177 denotes apical
T8712 19016-19026 _FRAGMENT denotes surface of
T8713 19042-19047 GO:0009986 denotes cells
T8714 19042-19047 CL_GO_EXT:cell denotes cells
T8715 19102-19109 GO:0005634 denotes nuclear
T8716 19126-19130 PR_EXT:000004182 denotes AQP2
T8717 19195-19204 CHEBI:42471 denotes forskolin
T8718 19257-19264 GO:0005634 denotes nuclear
T8719 19281-19285 PR_EXT:000004182 denotes AQP2
T8720 19314-19316 GO:0005783 denotes ER
T8721 19361-19365 PR_EXT:000004182 denotes AQP2
T8722 19372-19381 GO_PATO_EXT:biological_localization_process_or_quality denotes localizes
T8723 19389-19391 GO:0005783 denotes ER
T8724 19407-19412 CL_GO_EXT:cell denotes cells
T8725 19413-19424 GO:0009294 denotes transfected
T8726 19430-19434 PR_EXT:000004182 denotes Aqp2
T8727 19441-19445 SO_EXT:cDNA denotes cDNA
T8728 19451-19455 PR_EXT:000004182 denotes AQP2
T8729 19463-19465 GO:0005783 denotes ER
T8730 19466-19472 CHEBI_SO_EXT:molecular_indicator_or_label_or_marker_or_tag denotes marker
T8731 19474-19482 PR_EXT:000005005 denotes calnexin
T8732 19498-19510 GO_PATO_EXT:biological_localization_process_or_quality denotes localization
T8733 19514-19522 PR_EXT:000005005 denotes calnexin
T8734 19528-19532 PR_EXT:000004182 denotes AQP2
T8735 19593-19597 PR_EXT:000004182 denotes AQP2
T8736 19604-19611 CHEBI_PR_EXT:protein denotes protein
T8737 19614-19623 GO_PATO_EXT:biological_localization_process_or_quality denotes localized
T8738 19629-19637 PR_EXT:000005005 denotes calnexin
T8739 19690-19692 GO:0005783 denotes ER
T8740 19707-19711 PR_EXT:000004182 denotes AQP2
T8741 19761-19763 GO:0005783 denotes ER
T8742 19771-19773 GO:0005783 denotes ER
T8743 19842-19854 GO_MOP_EXT:glycosylation denotes glycosylated
T8744 19856-19860 PR_EXT:000004182 denotes AQP2
T8745 19870-19876 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T8746 19877-19884 UBERON:0002113 denotes kidneys
T8747 19902-19909 NCBITaxon:33208 denotes Animals
T8748 19931-19935 PR_EXT:000004182 denotes Aqp2
T8749 19941-19949 SO_EXT:sequence_alteration_entity_or_process denotes mutation
T8750 19977-19982 UBERON:0001088 denotes urine
T8751 19997-20002 UBERON:0001088 denotes urine
T8752 20082-20088 SO_EXT:0001023 denotes allele
T8753 20090-20094 PR_EXT:000004182 denotes AQP2
T8754 20125-20134 SO_EXT:wild_type_entity_or_quality denotes wild-type
T8755 20135-20142 CHEBI_PR_EXT:protein denotes protein
T8756 20146-20153 CL:0000023 denotes oocytes
T8757 20172-20188 GO:0051260 denotes homo-oligomerize
T8758 20197-20202 CL_GO_EXT:cell denotes cells
T8759 20220-20227 UBERON:0002113 denotes kidneys
T8760 20246-20253 NCBITaxon:33208 denotes animals
T8761 20303-20307 PR_EXT:000004182 denotes AQP2
T8762 20308-20315 CHEBI_PR_EXT:protein denotes protein
T8763 20363-20386 UBERON:0001232 denotes kidney collecting ducts
T8764 20392-20396 PR_EXT:000004182 denotes Aqp2
T8765 20402-20403 SO_EXT:normal_or_wild_type_or_present denotes +
T8766 20404-20408 NCBITaxon:10088 denotes mice
T8767 20450-20459 SO_EXT:wild_type_entity_or_quality denotes wild type
T8768 20473-20477 PR_EXT:000004182 denotes AQP2
T8769 20478-20490 GO_EXT:biological_movement_or_translocation_process denotes translocated
T8770 20509-20515 GO:0045177 denotes apical
T8771 20516-20520 CL_GO_EXT:cell denotes cell
T8772 20516-20528 GO:0009986 denotes cell surface
T8773 20534-20539 CHEBI:4450 denotes dDAVP
T8774 20558-20567 SO_EXT:wild_type_entity_or_quality denotes wild-type
T8775 20643-20649 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T8776 20650-20657 CHEBI_PR_EXT:protein denotes protein
T8777 20743-20752 SO_EXT:wild_type_entity_or_quality denotes wild-type
T8778 20753-20760 CHEBI_PR_EXT:protein denotes protein
T8779 20775-20787 GO_PATO_EXT:biological_localization_process_or_quality denotes localization
T8780 20795-20801 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T8781 20802-20809 CHEBI_PR_EXT:protein denotes protein
T8782 20884-20893 GO_EXT:biological_routing_or_transport denotes transport
T8783 20900-20912 GO_MOP_EXT:glycosylation denotes glycosylated
T8784 20925-20929 PR_EXT:000004182 denotes AQP2
T8785 20937-20941 CL_GO_EXT:cell denotes cell
T8786 20937-20949 GO:0009986 denotes cell surface
T8787 20953-20965 GO_MOP_EXT:glycosylation denotes glycosylated
T8788 21009-21018 SO_EXT:wild_type_entity_or_quality denotes wild-type
T8789 21019-21023 PR_EXT:000004182 denotes AQP2
T8790 21024-21031 CHEBI_PR_EXT:protein denotes protein
T8791 21036-21042 SO_EXT:sequence_rescue_process denotes rescue
T8792 21045-21058 GO_EXT:biological_translocation_entity_or_process denotes translocation
T8793 21069-21075 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T8794 21076-21083 CHEBI_PR_EXT:protein denotes protein
T8795 21085-21089 PR_EXT:000004182 denotes AQP2
T8796 21116-21125 GO:0010467 denotes expressed
T8797 21134-21139 CL_GO_EXT:cell denotes cells
T8798 22763-22779 UBERON:0001232 denotes collecting ducts
T8799 22785-22789 PR_EXT:000004182 denotes Aqp2
T8800 22795-22796 SO_EXT:normal_or_wild_type_or_present denotes +
T8801 22797-22801 NCBITaxon:10088 denotes mice
T8802 22807-22816 SO_EXT:wild_type_entity_or_quality denotes wild type
T8803 22821-22827 SO_EXT:sequence_rescue_process denotes rescue
T8804 22832-22838 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T8805 22839-22846 CHEBI_PR_EXT:protein denotes protein
T8806 22867-22878 GO_UBERON_EXT:cellular_component_or_cell_part denotes subcellular
T8807 22870-22878 CL_GO_EXT:cell denotes cellular
T8808 22895-22899 PR_EXT:000004182 denotes AQP2
T8809 22900-22907 CHEBI_PR_EXT:protein denotes protein
T8810 22971-22977 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T8811 22982-22991 SO_EXT:wild_type_entity_or_quality denotes wild-type
T8812 22992-23000 CHEBI_PR_EXT:protein denotes proteins
T8813 23004-23015 GO:0009294 denotes transfected
T8814 23021-23026 CL_GO_EXT:cell denotes cells
T8815 23045-23050 CL_GO_EXT:cell denotes cells
T8816 23058-23068 GO:0010467 denotes expressing
T8817 23069-23078 SO_EXT:wild_type_entity_or_quality denotes wild-type
T8818 23079-23083 PR_EXT:000004182 denotes AQP2
T8819 23101-23112 GO:0009294 denotes transfected
T8820 23122-23132 GO:0010467 denotes expression
T8821 23133-23143 SO_EXT:engineered_biological_sequence denotes constructs
T8822 23144-23152 SO_EXT:sequence_coding_function denotes encoding
T8823 23157-23163 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes tagged
T8824 23164-23173 SO_EXT:wild_type_entity_or_quality denotes wild-type
T8825 23174-23178 PR_EXT:000004182 denotes AQP2
T8826 23180-23184 PR_EXT:000004182 denotes AQP2
T8827 23206-23216 GO:0042571 denotes Antibodies
T8828 23250-23259 SO_EXT:wild_type_entity_or_quality denotes wild-type
T8829 23260-23264 PR_EXT:000004182 denotes AQP2
T8830 23270-23274 PR_EXT:000004182 denotes AQP2
T8831 23282-23286 PR_EXT:000004182 denotes AQP2
T8832 23313-23324 GO:0009294 denotes transfected
T8833 23369-23380 GO:0009294 denotes transfected
T8834 23391-23396 CL_GO_EXT:cell denotes cells
T8835 23404-23414 GO:0010467 denotes expressing
T8836 23415-23424 SO_EXT:wild_type_entity_or_quality denotes wild-type
T8837 23425-23429 PR_EXT:000004182 denotes AQP2
T8838 23478-23487 GO:0016020 denotes membranes
T8839 23500-23509 SO_EXT:wild_type_entity_or_quality denotes wild-type
T8840 23510-23514 PR_EXT:000004182 denotes AQP2
T8841 23531-23540 GO:0010467 denotes expressed
T8842 23589-23598 SO_EXT:wild_type_entity_or_quality denotes wild-type
T8843 23603-23609 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T8844 23610-23618 CHEBI_PR_EXT:protein denotes proteins
T8845 23649-23653 CL_GO_EXT:cell denotes cell
T8846 23689-23695 SO_EXT:sequence_rescue_process denotes rescue
T8847 23700-23712 GO_PATO_EXT:biological_localization_process_or_quality denotes localization
T8848 23716-23722 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T8849 23723-23730 CHEBI_PR_EXT:protein denotes protein
T8850 23770-23775 CL_GO_EXT:cell denotes cells
T8851 23783-23794 GO:0009294 denotes transfected
T8852 23800-23809 SO_EXT:wild_type_entity_or_quality denotes wild-type
T8853 23810-23814 PR_EXT:000004182 denotes AQP2
T8854 23815-23825 GO:0010467 denotes expressing
T8855 23826-23832 SO_EXT:0000440 denotes vector
T8856 23847-23853 SO_EXT:0000440 denotes vector
T8857 23887-23898 GO:0009294 denotes transfected
T8858 23899-23903 PR_EXT:000004182 denotes AQP2
T8859 23911-23915 PR_EXT:000004182 denotes AQP2
T8860 23926-23936 GO:0010467 denotes expression
T8861 23937-23947 SO_EXT:engineered_biological_sequence denotes constructs
T8862 23949-23953 PR_EXT:000004182 denotes AQP2
T8863 23958-23967 GO_PATO_EXT:biological_localization_process_or_quality denotes localized
T8864 23975-23981 GO:0045177 denotes apical
T8865 23982-23989 GO:0009986 denotes surface
T8866 23995-24004 CHEBI:42471 denotes forskolin
T8867 24044-24055 GO:0009294 denotes transfected
T8868 24061-24067 SO_EXT:0000440 denotes vector
T8869 24073-24078 CL_GO_EXT:cell denotes cells
T8870 24118-24127 SO_EXT:wild_type_entity_or_quality denotes wild-type
T8871 24128-24132 PR_EXT:000004182 denotes AQP2
T8872 24133-24138 CL_GO_EXT:cell denotes cells
T8873 24165-24169 PR_EXT:000004182 denotes AQP2
T8874 24186-24195 GO:0010467 denotes expressed
T8875 24209-24221 GO:0009294 denotes transfection
T8876 24227-24233 SO_EXT:0000440 denotes vector
T8877 24239-24244 CL_GO_EXT:cell denotes cells
T8878 24263-24274 GO:0005737 denotes cytoplasmic
T8879 24326-24335 GO:0010467 denotes expressed
T8880 24339-24348 SO_EXT:wild_type_entity_or_quality denotes wild-type
T8881 24349-24353 PR_EXT:000004182 denotes AQP2
T8882 24354-24359 CL_GO_EXT:cell denotes cells
T8883 24370-24374 PR_EXT:000004182 denotes AQP2
T8884 24385-24394 GO_PATO_EXT:biological_localization_process_or_quality denotes localized
T8885 24402-24408 GO:0045177 denotes apical
T8886 24409-24416 GO:0009986 denotes surface
T8887 24525-24530 CL_GO_EXT:cell denotes cells
T8888 24545-24557 GO:0009294 denotes transfection
T8889 24577-24588 GO:0009294 denotes transfected
T8890 24589-24593 CL_GO_EXT:cell denotes cell
T8891 24609-24621 GO_PATO_EXT:biological_localization_process_or_quality denotes localization
T8892 24636-24646 GO:0010467 denotes expressing
T8893 24647-24656 SO_EXT:wild_type_entity_or_quality denotes wild-type
T8894 24657-24661 PR_EXT:000004182 denotes AQP2
T8895 24672-24678 GO:0045177 denotes apical
T8896 24684-24693 CHEBI:42471 denotes forskolin
T8897 24725-24735 GO:0010467 denotes expression
T8898 24755-24764 SO_EXT:wild_type_entity_or_quality denotes wild-type
T8899 24765-24769 PR_EXT:000004182 denotes AQP2
T8900 24788-24792 PR_EXT:000004182 denotes AQP2
T8901 24807-24811 CL_GO_EXT:cell denotes cell
T8902 24818-24830 GO_PATO_EXT:biological_localization_process_or_quality denotes localization
T8903 24834-24843 SO_EXT:wild_type_entity_or_quality denotes wild-type
T8904 24844-24848 PR_EXT:000004182 denotes AQP2
T8905 24876-24880 PR_EXT:000004182 denotes AQP2
T8906 24902-24908 GO:0045177 denotes apical
T8907 24914-24923 CHEBI:42471 denotes forskolin
T8908 24971-24975 CL_GO_EXT:cell denotes cell
T8909 24983-24987 PR_EXT:000004182 denotes AQP2
T8910 25009-25018 GO_PATO_EXT:biological_localization_process_or_quality denotes localized
T8911 25026-25032 GO:0045177 denotes apical
T8912 25033-25040 GO:0009986 denotes surface
T8913 25057-25069 GO_PATO_EXT:biological_localization_process_or_quality denotes localization
T8914 25073-25077 PR_EXT:000004182 denotes AQP2
T8915 25105-25111 GO:0045177 denotes apical
T8916 25117-25126 SO_EXT:wild_type_entity_or_quality denotes wild-type
T8917 25127-25131 PR_EXT:000004182 denotes AQP2
T8918 25141-25150 GO:0010467 denotes expressed
T8919 25177-25190 CHEBI_EXT:biochemical denotes biochemically
T8920 25195-25206 GO:0009294 denotes transfected
T8921 25216-25220 CL_GO_EXT:cell denotes cell
T8922 25228-25237 SO_EXT:wild_type_entity_or_quality denotes wild-type
T8923 25238-25242 PR_EXT:000004182 denotes AQP2
T8924 25246-25252 SO_EXT:0000440 denotes vector
T8925 25270-25282 MOP:0000093 denotes biotinylated
T8926 25283-25290 GO:0009986 denotes surface
T8927 25291-25299 CHEBI_PR_EXT:protein denotes proteins
T8928 25306-25315 CHEBI:42471 denotes forskolin
T8929 25350-25362 MOP:0000093 denotes biotinylated
T8930 25363-25371 CHEBI_PR_EXT:protein denotes proteins
T8931 25385-25389 PR_EXT:000004182 denotes AQP2
T8932 25403-25412 GO:0010467 denotes expressed
T8933 25443-25447 CL_GO_EXT:cell denotes cell
T8934 25472-25477 CL_GO_EXT:cell denotes cells
T8935 25487-25499 MOP:0000093 denotes biotinylated
T8936 25510-25519 SO_EXT:wild_type_entity_or_quality denotes wild-type
T8937 25520-25524 PR_EXT:000004182 denotes AQP2
T8938 25533-25542 GO:0010467 denotes expressed
T8939 25555-25562 GO:0009986 denotes surface
T8940 25563-25575 MOP:0000093 denotes biotinylated
T8941 25589-25593 CL_GO_EXT:cell denotes cell
T8942 25589-25601 GO:0009986 denotes cell surface
T8943 25602-25610 CHEBI_PR_EXT:protein denotes proteins
T8944 25629-25635 CHEBI:15956 denotes biotin
T8945 25665-25669 PR_EXT:000004182 denotes AQP2
T8946 25679-25690 GO_EXT:biological_routing_or_transport denotes transported
T8947 25698-25702 CL_GO_EXT:cell denotes cell
T8948 25698-25710 GO:0009986 denotes cell surface
T8949 25716-25725 SO_EXT:wild_type_entity_or_quality denotes wild-type
T8950 25726-25730 PR_EXT:000004182 denotes AQP2
T13940 25775-25779 PR_EXT:000004182 denotes Aqp2
T13941 25790-25794 NCBITaxon:10088 denotes mice
T13942 25810-25814 GO:0040007 denotes grow
T13943 25819-25828 GO:0000003 denotes reproduce
T13944 25909-25914 UBERON:0001088 denotes urine
T13945 25937-25942 UBERON:0001088 denotes urine
T13946 25954-25959 CHEBI:15377 denotes water
T13947 25960-25966 GO:0007631 denotes intake
T13948 25995-26000 NCBITaxon:10088 denotes mouse
T13949 26041-26047 NCBITaxon:9606 denotes humans
T13950 26066-26075 SO_EXT:sequence_alteration_entity_or_process denotes mutations
T13951 26079-26084 PR_EXT:000001683 denotes Avpr2
T13952 26088-26092 PR_EXT:000004182 denotes Aqp2
T13953 26110-26111 GO:0000805 denotes X
T13954 26119-26124 PR_EXT:000001683 denotes Avpr2
T13955 26125-26129 SO_EXT:0000704 denotes gene
T13956 26133-26137 NCBITaxon:10088 denotes mice
T13957 26173-26177 PATO_UBERON_EXT:male_or_bearer_of_maleness denotes male
T13958 26190-26198 PATO_UBERON_EXT:neonate_or_newborn denotes neonates
T13959 26243-26249 UBERON:0007023 denotes adults
T13960 26257-26261 NCBITaxon:10088 denotes mice
T13961 26262-26266 GO:0016265 denotes died
T13962 26282-26287 GO:0007567 denotes birth
T13963 26293-26298 UBERON:0007023 denotes adult
T13964 26312-26319 PATO_UBERON_EXT:female_or_bearer_of_femaleness denotes females
T13965 26360-26367 UBERON:0001088 denotes urinary
T13966 26379-26384 CHEBI:15377 denotes water
T13967 26385-26391 GO:0007631 denotes intake
T13968 26406-26411 UBERON:0001088 denotes urine
T13969 26440-26445 NCBITaxon:10088 denotes mouse
T13970 26446-26450 PR_EXT:000004182 denotes Aqp2
T13971 26451-26455 SO_EXT:0000704 denotes gene
T13972 26495-26500 NCBITaxon:9606 denotes human
T13973 26517-26525 SO_EXT:sequence_alteration_entity_or_process denotes mutation
T13974 26570-26574 NCBITaxon:10088 denotes mice
T13975 26589-26594 UBERON:0001088 denotes urine
T13976 26645-26650 GO:0016265 denotes death
T13977 26666-26671 GO:0007567 denotes birth
T13978 26684-26688 PR_EXT:000004182 denotes AQP2
T13979 26700-26708 GO_PATO_EXT:biological_localization_process_or_quality denotes localize
T13980 26742-26747 CL_GO_EXT:cell denotes cells
T13981 26770-26785 UBERON:0001232 denotes collecting duct
T13982 26833-26842 CHEBI_EXT:polyatomic_entity_or_group denotes molecular
T13983 26868-26872 NCBITaxon:10088 denotes mice
T13984 26912-26916 PR_EXT:000004182 denotes Aqp2
T13985 26933-26937 SO_EXT:0000704 denotes gene
T13986 26962-26966 NCBITaxon:10088 denotes mice
T13987 26993-26997 PR_EXT:000004182 denotes AQP2
T13988 27028-27033 CHEBI:15377 denotes water
T13989 27028-27046 GO:0006833 denotes water transporting
T13990 27073-27076 CHEBI_PR_EXT:arginine_vasopressin denotes AVP
T13991 27102-27107 CHEBI:15377 denotes water
T13992 27143-27147 PR_EXT:000004182 denotes AQP2
T13993 27168-27174 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T13994 27175-27182 NCBITaxon:33208 denotes animals
T13995 27199-27207 GO_EXT:reaction_or_response denotes response
T13996 27211-27216 CHEBI:4450 denotes dDAVP
T13997 27227-27232 CHEBI:4450 denotes dDAVP
T13998 27244-27249 UBERON:0001088 denotes urine
T13999 27298-27304 UBERON:0002113 denotes kidney
T14000 27316-27320 PR_EXT:000004182 denotes AQP2
T14001 27348-27357 GO_EXT:biological_routing_or_transport denotes transport
T14002 27358-27363 CHEBI:15377 denotes water
T14003 27385-27393 GO_PATO_EXT:biological_localization_process_or_quality denotes localize
T14004 27401-27407 GO:0045177 denotes apical
T14005 27408-27412 CL_GO_EXT:cell denotes cell
T14006 27408-27420 GO:0009986 denotes cell surface
T14007 27427-27432 CHEBI:4450 denotes dDAVP
T14008 27470-27474 PR_EXT:000004182 denotes AQP2
T14009 27532-27538 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T14010 27539-27546 CHEBI_PR_EXT:protein denotes protein
T14011 27565-27569 CL_GO_EXT:cell denotes cell
T14012 27565-27577 GO:0009986 denotes cell surface
T14013 27583-27590 GO:0009986 denotes surface
T14014 27591-27604 MOP:0000093 denotes biotinylation
T14015 27645-27651 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T14016 27652-27659 CHEBI_PR_EXT:protein denotes protein
T14017 27672-27679 GO:0009986 denotes surface
T14018 27771-27778 CHEBI_PR_EXT:protein denotes protein
T14019 27857-27863 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T14020 27864-27871 CHEBI_PR_EXT:protein denotes protein
T14021 27897-27899 GO:0005783 denotes ER
T14022 27946-27955 SO_EXT:wild_type_entity_or_quality denotes wild-type
T14023 27957-27963 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T14024 27964-27971 CHEBI_PR_EXT:protein denotes protein
T14025 27996-28003 CHEBI:37684 denotes mannose
T14026 28010-28022 GO_MOP_EXT:glycosylation denotes glycosylated
T14027 28057-28069 GO_MOP_EXT:glycosylation denotes glycosylated
T14028 28091-28103 GO_MOP_EXT:glycosylation denotes glycosylated
T14029 28174-28181 CHEBI_PR_EXT:protein denotes protein
T14030 28220-28226 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T14031 28227-28234 CHEBI_PR_EXT:protein denotes protein
T14032 28238-28249 GO_EXT:biological_routing_or_transport denotes transported
T14033 28261-28263 GO:0005783 denotes ER
T14034 28304-28316 GO_PATO_EXT:biological_localization_process_or_quality denotes localization
T14035 28320-28324 PR_EXT:000004182 denotes AQP2
T14036 28340-28342 GO:0005783 denotes ER
T14037 28343-28350 CHEBI_PR_EXT:protein denotes protein
T14038 28351-28359 PR_EXT:000005005 denotes calnexin
T14039 28363-28374 GO:0009294 denotes transfected
T14040 28380-28385 CL_GO_EXT:cell denotes cells
T14041 28416-28422 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T14042 28423-28430 CHEBI_PR_EXT:protein denotes protein
T14043 28449-28451 GO:0005783 denotes ER
T14044 28483-28485 GO:0005783 denotes ER
T14045 28498-28506 GO_EXT:reaction_or_response denotes response
T14046 28510-28515 CHEBI:4450 denotes dDAVP
T14047 28548-28560 GO_MOP_EXT:glycosylation denotes glycosylated
T14048 28561-28568 CHEBI_PR_EXT:protein denotes protein
T14049 28572-28578 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T14050 28579-28586 NCBITaxon:33208 denotes animals
T14051 28596-28608 _FRAGMENT denotes transport of
T14052 28630-28637 GO:0015031 denotes protein
T14053 28623-28629 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T14054 28630-28637 CHEBI_PR_EXT:protein denotes protein
T14055 28649-28651 GO:0005783 denotes ER
T14056 28660-28665 CL_GO_EXT:cell denotes cells
T14057 28706-28710 PR_EXT:000004182 denotes AQP2
T14058 28720-28727 GO:0006457 denotes folding
T14059 28776-28781 CHEBI:15377 denotes water
T14060 28776-28794 GO:0006833 denotes water transporting
T14061 28874-28880 GO_EXT:biological_growth_entity_or_process denotes growth
T14062 28884-28890 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T14063 28891-28898 NCBITaxon:33208 denotes animals
T14064 28934-28936 GO:0005783 denotes ER
T14065 28953-28957 PR_EXT:000004182 denotes AQP2
T14066 28995-29002 CHEBI:37684 denotes mannose
T14067 29003-29015 GO_MOP_EXT:glycosylation denotes glycosylated
T14068 29031-29038 CHEBI:37684 denotes mannose
T14069 29044-29059 CHEBI:50699 denotes oligosaccharide
T14070 29076-29078 GO:0005783 denotes ER
T14071 29092-29100 SO_EXT:sequence_alteration_process denotes modified
T14072 29123-29138 GO:0005794 denotes Golgi apparatus
T14073 29170-29177 CHEBI:37684 denotes mannose
T14074 29178-29190 GO_MOP_EXT:glycosylation denotes glycosylated
T14075 29199-29203 PR_EXT:000004182 denotes AQP2
T14076 29259-29261 GO:0005783 denotes ER
T14077 29278-29292 CHEBI:50699 denotes oligosaccharyl
T14078 29278-29304 GO_EXT:0004576 denotes oligosaccharyl transferase
T14079 29321-29333 GO_PATO_EXT:biological_localization_process_or_quality denotes localization
T14080 29337-29341 PR_EXT:000004182 denotes AQP2
T14081 29379-29385 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T14082 29386-29390 NCBITaxon:10088 denotes mice
T14083 29441-29445 NCBITaxon:10088 denotes mice
T14084 29476-29491 GO_PATO_EXT:physiological_process_or_quality denotes Physiologically
T14085 29506-29510 NCBITaxon:10088 denotes mice
T14086 29581-29590 SO_EXT:wild_type_entity_or_quality denotes wild type
T14087 29612-29619 UBERON:0002113 denotes kidneys
T14088 29677-29686 SO_EXT:wild_type_entity_or_quality denotes wild-type
T14089 29687-29694 CHEBI_PR_EXT:protein denotes protein
T14090 29767-29769 GO:0005783 denotes ER
T14091 29779-29785 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T14092 29786-29793 CHEBI_PR_EXT:protein denotes protein
T14093 29824-29828 PR_EXT:000004182 denotes Aqp2
T14094 29834-29835 SO_EXT:normal_or_wild_type_or_present denotes +
T14095 29836-29843 NCBITaxon:33208 denotes animals
T14096 29862-29868 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T14097 29869-29876 CHEBI_PR_EXT:protein denotes protein
T14098 29886-29893 SO_EXT:sequence_rescue_process denotes rescued
T14099 29901-29910 SO_EXT:wild_type_entity_or_quality denotes wild-type
T14100 29911-29918 CHEBI_PR_EXT:protein denotes protein
T14101 29987-29993 SO_EXT:0001023 denotes allele
T14102 29997-30001 PR_EXT:000004182 denotes Aqp2
T14103 30028-30039 GO_EXT:biological_movement_or_translocation_process denotes translocate
T14104 30045-30054 GO:0010467 denotes expressed
T14105 30073-30078 CL_GO_EXT:cell denotes cells
T14106 30108-30117 SO_EXT:wild_type_entity_or_quality denotes wild-type
T14107 30118-30125 CHEBI_PR_EXT:protein denotes protein
T14108 30130-30139 GO_PATO_EXT:biological_localization_process_or_quality denotes localizes
T14109 30197-30201 PR_EXT:000004182 denotes Aqp2
T14110 30207-30208 SO_EXT:normal_or_wild_type_or_present denotes +
T14111 30209-30213 NCBITaxon:10088 denotes mice
T14112 30235-30239 PR_EXT:000004182 denotes AQP2
T14113 30264-30273 SO_EXT:wild_type_entity_or_quality denotes wild-type
T14114 30274-30278 PR_EXT:000004182 denotes AQP2
T14115 30303-30312 GO:0010467 denotes expressed
T14116 30318-30327 SO_EXT:wild_type_entity_or_quality denotes wild-type
T14117 30328-30335 CHEBI_PR_EXT:protein denotes protein
T14118 30337-30341 PR_EXT:000004182 denotes AQP2
T14119 30362-30366 CL_GO_EXT:cell denotes cell
T14120 30362-30374 GO:0009986 denotes cell surface
T14121 30465-30471 SO_EXT:0001023 denotes allele
T14122 30473-30481 SO_EXT:sequence_coding_function denotes encoding
T14123 30482-30486 PR_EXT:000004182 denotes AQP2
T14124 30524-30533 SO_EXT:wild_type_entity_or_quality denotes wild-type
T14125 30534-30538 PR_EXT:000004182 denotes AQP2
T14126 30562-30566 PR_EXT:000004182 denotes AQP2
T14127 30596-30605 SO_EXT:wild_type_entity_or_quality denotes wild-type
T14128 30606-30613 CHEBI_PR_EXT:protein denotes protein
T14129 30643-30652 GO_EXT:tetrameric_macromolecular_complex denotes tetramers
T14130 30671-30680 SO_EXT:sequence_rescue_process denotes rescuable
T14131 30681-30687 SO_EXT:0001023 denotes allele
T14132 30735-30742 UBERON:0002113 denotes kidneys
T14133 30757-30761 PR_EXT:000004182 denotes Aqp2
T14134 30773-30777 NCBITaxon:10088 denotes mice
T14135 30793-30799 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T14136 30800-30810 GO:0010467 denotes expressing
T14137 30811-30826 UBERON:0001232 denotes collecting duct
T14138 30811-30832 CL:1001225 denotes collecting duct cells
T14139 30827-30832 CL_GO_EXT:cell denotes cells
T14140 30849-30854 CHEBI:15377 denotes water
T14141 30905-30927 GO:0016324 denotes apical plasma membrane
T14142 30931-30939 GO_EXT:reaction_or_response denotes response
T14143 30943-30948 CHEBI:4450 denotes dDAVP
T14144 31061-31065 PR_EXT:000004182 denotes Aqp2
T14145 31066-31075 SO_EXT:sequence_alteration_entity_or_process denotes mutations
T14146 31077-31089 GO:0009294 denotes Transfection
T14147 31100-31105 CL_GO_EXT:cell denotes cells
T14148 31124-31128 PR_EXT:000004182 denotes Aqp2
T14149 31129-31138 SO_EXT:sequence_alteration_entity_or_process denotes mutations
T14150 31166-31171 NCBITaxon:9606 denotes human
T14151 31172-31179 SO_EXT:0001023 denotes alleles
T14152 31195-31206 GO_UBERON_EXT:cellular_component_or_cell_part denotes subcellular
T14153 31198-31206 CL_GO_EXT:cell denotes cellular
T14154 31207-31219 GO_PATO_EXT:biological_localization_process_or_quality denotes localization
T14155 31260-31271 GO_EXT:biological_movement_or_translocation_process denotes translocate
T14156 31279-31294 GO:0005886 denotes plasma membrane
T14157 31305-31312 GO:0006457 denotes folding
T14158 31331-31333 GO:0005783 denotes ER
T14159 31350-31361 GO_EXT:biological_movement_or_translocation_process denotes translocate
T14160 31365-31373 GO_EXT:reaction_or_response denotes response
T14161 31377-31382 CHEBI:4450 denotes dDAVP
T14162 31418-31427 GO:0030849 denotes autosomal
T14163 31520-31525 NCBITaxon:9606 denotes human
T14164 31536-31541 NCBITaxon:10088 denotes mouse
T14165 31567-31571 PR_EXT:000004182 denotes Aqp2
T14166 31572-31578 SO_EXT:0001023 denotes allele
T14167 31591-31598 SO_EXT:sequence_rescue_process denotes rescued
T14168 31653-31657 SO_EXT:0000704 denotes gene
T14169 31691-31702 GO_UBERON_EXT:cellular_component_or_cell_part denotes subcellular
T14170 31694-31702 CL_GO_EXT:cell denotes cellular
T14171 31703-31715 GO_PATO_EXT:biological_localization_process_or_quality denotes localization
T15499 31755-31758 CHEBI:23995 denotes ENU
T15500 31759-31763 NCBITaxon:10088 denotes mice
T15501 31777-31780 CHEBI:23995 denotes ENU
T15502 31801-31805 NCBITaxon:10088 denotes mice
T15503 31840-31844 NCBITaxon:10088 denotes Mice
T15504 31886-31893 NCBITaxon:33208 denotes animals
T15505 32001-32007 NCBITaxon:33208 denotes animal
T15506 32161-32167 NCBITaxon:33208 denotes Animal
T15875 32193-32203 SO_EXT:engineered_biological_sequence denotes Constructs
T15876 32218-32233 SO_EXT:coding_sequence denotes coding sequence
T15877 32237-32242 NCBITaxon:10088 denotes mouse
T15878 32243-32247 PR_EXT:000004182 denotes AQP2
T15879 32262-32267 SO_EXT:sequence_cloned_entity denotes clone
T15880 32302-32309 SO_EXT:0000155 denotes plasmid
T15881 32334-32341 GO:0006266 denotes ligated
T15882 32417-32425 SO_EXT:sequence_alteration_entity_or_process denotes mutation
T15883 32449-32457 GO_EXT:biological_direction_or_guidance denotes directed
T15884 32458-32469 SO_EXT:sequence_alteration_process denotes mutagenesis
T15885 32537-32552 CHEBI_SO_EXT:oligonucleotide denotes oligonucleotide
T15886 32596-32605 SO:0000077 denotes antisense
T15887 32606-32621 CHEBI_SO_EXT:oligonucleotide denotes oligonucleotide
T15888 32677-32684 SO_EXT:sequence_fusion_entity_or_process denotes fusions
T15889 32688-32692 PR_EXT:000004182 denotes AQP2
T15890 32710-32714 PR_EXT:000004182 denotes AQP2
T15891 32715-32724 SO_EXT:engineered_biological_sequence denotes construct
T15892 32761-32768 SO_EXT:0000112 denotes primers
T15893 32830-32840 SO_EXT:0000319 denotes stop codon
T15894 32844-32848 PR_EXT:000004182 denotes AQP2
T15895 32889-32894 PR_EXT:P23940 denotes BamHI
T15896 32899-32906 GO:0006266 denotes ligated
T15897 32987-32995 SO_EXT:sequence_alteration_entity_or_process denotes mutation
T15898 33026-33035 CHEBI_EXT:25435 denotes mutagenic
T15899 33036-33052 CHEBI_SO_EXT:oligonucleotide denotes oligonucleotides
T16380 33055-33059 CL_GO_EXT:cell denotes Cell
T16381 33093-33097 CL_GO_EXT:cell denotes cell
T16382 33110-33115 CL_GO_EXT:cell denotes cells
T16383 33295-33305 CHEBI:17334 denotes penicillin
T16384 33324-33336 CHEBI:17076 denotes streptomycin
T16385 33352-33355 CHEBI:16526 denotes CO2
T16386 33381-33385 CL_GO_EXT:cell denotes cell
T16387 33393-33398 CL_GO_EXT:cell denotes cells
T16388 33404-33415 GO:0009294 denotes transfected
T16389 33471-33481 GO:0010467 denotes expression
T16390 33482-33492 SO_EXT:engineered_biological_sequence denotes constructs
T16391 33505-33514 SO_EXT:wild_type_entity_or_quality denotes wild-type
T16392 33515-33519 PR_EXT:000004182 denotes AQP2
T16393 33521-33525 PR_EXT:000004182 denotes AQP2
T16394 33539-33545 SO_EXT:0000667 denotes insert
T16395 33575-33579 CHEBI:42768 denotes G418
T16396 33686-33696 CHEBI_EXT:33282 denotes antibiotic
T16397 33747-33760 GO:0009294 denotes transfections
T16398 33801-33806 CL_GO_EXT:cell denotes cells
T16667 33859-33863 PR_EXT:000004182 denotes Aqp2
T16668 33882-33886 NCBITaxon:10088 denotes mice
T16669 33892-33897 SO_EXT:0000147 denotes exons
T16670 33901-33905 PR_EXT:000004182 denotes Aqp2
T16671 33926-33931 NCBITaxon:10088 denotes mouse
T16672 33932-33943 SO_EXT:genomic_DNA denotes genomic DNA
T16673 33940-33943 CHEBI_SO_EXT:DNA denotes DNA
T16674 33975-33979 SO_EXT:0000147 denotes exon
T16675 34006-34013 SO_EXT:0000112 denotes primers
T16946 34074-34079 UBERON:0001088 denotes Urine
T16947 34100-34105 UBERON:0001088 denotes urine
T16948 34148-34153 UBERON:0007023 denotes adult
T16949 34154-34158 NCBITaxon:10088 denotes mice
T16950 34170-34179 GO:0008152 denotes Metabolic
T16951 34253-34258 UBERON:0001088 denotes urine
T16952 34278-34283 UBERON:0001088 denotes Urine
T16953 34405-34410 UBERON:0001088 denotes Urine
T16954 34462-34472 UBERON:0001179 denotes peritoneal
T16955 34486-34491 CHEBI:4450 denotes dDAVP
T16956 34505-34509 NCBITaxon:10088 denotes Mice
T16957 34535-34540 CHEBI:4450 denotes dDAVP
T16958 34578-34583 UBERON:0001088 denotes Urine
T17254 34669-34675 UBERON:0002113 denotes Kidney
T17255 34676-34684 GO:0016020 denotes membrane
T17256 34704-34709 NCBITaxon:10088 denotes mouse
T17257 34710-34717 UBERON:0002113 denotes kidneys
T17258 34744-34748 CHEBI:9754 denotes Tris
T17259 34766-34773 CHEBI:17992 denotes sucrose
T17260 34784-34788 CHEBI_EXT:EDTA denotes EDTA
T17261 34800-34819 CHEBI_GO_EXT:peptidase_or_protease_or_proteinase_inhibitor denotes protease inhibitors
T17262 35028-35037 GO:0016020 denotes membranes
T17263 35067-35073 CHEBI_CHMO_EXT:buffer_solution denotes buffer
T17264 35079-35086 CHEBI_PR_EXT:protein denotes protein
T17594 35152-35158 UBERON:0002113 denotes Kidney
T17595 35159-35167 GO:0016020 denotes membrane
T17596 35209-35212 CHEBI:8984 denotes SDS
T17597 35213-35227 CHEBI_EXT:polyacrylamide denotes polyacrylamide
T17598 35253-35267 CHEBI:53325 denotes nitrocellulose
T17599 35278-35287 GO:0016020 denotes Membranes
T17600 35310-35313 CHEBI_UBERON_EXT:triglyceride_or_adipose_tissue denotes fat
T17601 35322-35326 CHEBI:9754 denotes Tris
T17602 35327-35335 CHEBI_CHMO_EXT:buffer_process denotes buffered
T17603 35336-35342 CHEBI_EXT:saline_solution denotes saline
T17604 35354-35362 CHEBI:53424 denotes Tween 20
T17605 35422-35426 PR_EXT:000004182 denotes AQP2
T17606 35438-35446 GO:0042571 denotes antibody
T17607 35524-35533 GO:0016020 denotes Membranes
T17608 35578-35588 MOP:0000779 denotes conjugated
T17609 35589-35595 NCBITaxon:9793 denotes donkey
T17610 35601-35605 NCBITaxon:9925 denotes goat
T17611 35606-35614 GO:0042571 denotes antibody
T17612 35616-35625 GO:0016020 denotes Membranes
T17613 35690-35697 CHEBI_EXT:33893 denotes reagent
T17898 35783-35789 UBERON:0002113 denotes Kidney
T17899 35790-35799 GO:0016020 denotes membranes
T17900 35832-35848 CHEBI:37586 denotes sodium phosphate
T17901 35864-35867 CHEBI:8984 denotes SDS
T17902 35879-35896 CHEBI:41218 denotes β-mercaptoethanol
T17903 36086-36092 CHEBI_CHMO_EXT:buffer_solution denotes buffer
T17904 36100-36109 CHEBI_EXT:33893 denotes reactants
T18724 36176-36189 MOP:0000093 denotes biotinylation
T18725 36198-36203 CL_GO_EXT:cell denotes cells
T18726 36210-36215 CL_GO_EXT:cell denotes cells
T18727 36223-36233 GO:0010467 denotes expressing
T18728 36234-36243 SO_EXT:wild_type_entity_or_quality denotes wild-type
T18729 36244-36248 PR_EXT:000004182 denotes AQP2
T18730 36250-36255 GO:0040007 denotes grown
T18731 36278-36289 GO:0009294 denotes transfected
T18732 36301-36310 SO_EXT:wild_type_entity_or_quality denotes wild-type
T18733 36311-36315 PR_EXT:000004182 denotes AQP2
T18734 36323-36327 PR_EXT:000004182 denotes AQP2
T18735 36338-36344 SO_EXT:0000440 denotes vector
T18736 36356-36361 CL_GO_EXT:cell denotes cells
T18737 36388-36392 CHEBI:9754 denotes Tris
T18738 36408-36412 CHEBI_EXT:EDTA denotes EDTA
T18739 36425-36432 CHEBI:17992 denotes sucrose
T18740 36521-36530 GO:0016020 denotes membranes
T18741 36560-36566 CHEBI_CHMO_EXT:buffer_solution denotes buffer
T18742 36585-36604 CHEBI:9177 denotes sodium deoxycholate
T18743 36656-36665 GO:0016020 denotes membranes
T18744 36716-36724 GO:0016020 denotes membrane
T18745 36749-36758 GO:0016020 denotes membranes
T18746 36806-36812 CHEBI_CHMO_EXT:buffer_solution denotes buffer
T18747 36849-36853 UBERON:0001977 denotes sera
T18748 36881-36888 CHEBI_PR_EXT:protein denotes protein
T18749 36881-36890 PR_EXT:000029158 denotes protein A
T18750 36953-36961 CHEBI_PR_EXT:protein denotes proteins
T18751 36982-36988 CHEBI_CHMO_EXT:buffer_solution denotes buffer
T18752 37028-37034 CHEBI_CHMO_EXT:buffer_solution denotes buffer
T18753 37054-37062 GO:0016020 denotes membrane
T18754 37119-37123 CL_GO_EXT:cell denotes Cell
T18755 37119-37131 GO:0009986 denotes Cell surface
T18756 37132-37145 MOP:0000093 denotes biotinylation
T18757 37196-37200 PR_EXT:000004182 denotes AQP2
T18758 37212-37223 GO:0009294 denotes transfected
T18759 37234-37239 CL_GO_EXT:cell denotes cells
T18760 37247-37257 GO:0010467 denotes expressing
T18761 37258-37267 SO_EXT:wild_type_entity_or_quality denotes wild-type
T18762 37268-37272 PR_EXT:000004182 denotes AQP2
T18763 37277-37282 CL_GO_EXT:cell denotes cells
T18764 37300-37306 SO_EXT:0000440 denotes vector
T18765 37337-37349 GO:0009294 denotes transfection
T18766 37351-37356 CL_GO_EXT:cell denotes cells
T18767 37378-37387 CHEBI:42471 denotes forskolin
T18768 37389-37400 GO_EXT:trypsinization denotes trypsinized
T18769 37440-37445 CL_GO_EXT:cell denotes cells
T18770 37489-37495 CHEBI:15956 denotes biotin
T18771 37551-37556 CL_GO_EXT:cell denotes Cells
T18772 37583-37587 CHEBI:9754 denotes Tris
T18773 37631-37640 GO:0016020 denotes membranes
T18774 37703-37712 GO:0016020 denotes membranes
T18775 37744-37753 GO_EXT:biological_movement_or_translocation_process denotes mobilized
T18776 37832-37840 CHEBI_PR_EXT:protein denotes proteins
T18777 37861-37867 CHEBI_CHMO_EXT:buffer_solution denotes buffer
T18778 37899-37905 CHEBI_CHMO_EXT:buffer_solution denotes buffer
T18779 37913-37918 CL_GO_EXT:cell denotes cells
T18780 37927-37939 MOP:0000093 denotes biotinylated
T18781 37982-37990 GO:0042571 denotes antibody
T18782 37994-37998 PR_EXT:000004182 denotes AQP2
T19567 38001-38007 UBERON:0002113 denotes Kidney
T19568 38036-38041 NCBITaxon:10088 denotes mouse
T19569 38042-38049 UBERON:0002113 denotes kidneys
T19570 38068-38077 CHEBI_EXT:phosphate denotes phosphate
T19571 38078-38086 CHEBI_CHMO_EXT:buffer_process denotes buffered
T19572 38087-38095 CHEBI:16842 denotes formalin
T19573 38106-38113 UBERON:0002113 denotes Kidneys
T19574 38184-38191 CHEBI_EXT:59132 denotes antigen
T19575 38214-38228 CHEBI:53258 denotes sodium citrate
T19576 38302-38306 PR_EXT:000004183 denotes AQP3
T19577 38320-38324 PR_EXT:000004182 denotes AQP2
T19578 38356-38362 NCBITaxon:9793 denotes donkey
T19579 38363-38368 UBERON:0001977 denotes serum
T19580 38381-38385 NCBITaxon:9925 denotes goat
T19581 38391-38395 PR_EXT:000004183 denotes AQP3
T19582 38396-38404 GO:0042571 denotes antibody
T19583 38497-38511 CHEBI:52661 denotes AlexaFluor 488
T19584 38512-38522 MOP:0000779 denotes conjugated
T19585 38523-38529 NCBITaxon:9793 denotes donkey
T19586 38535-38539 NCBITaxon:9925 denotes goat
T19587 38540-38548 GO:0042571 denotes antibody
T19588 38550-38559 CHEBI_EXT:polyatomic_entity_or_group denotes Molecular
T19589 38560-38566 CHEBI_SO_EXT:molecular_probe denotes Probes
T19590 38643-38650 NCBITaxon:9031 denotes chicken
T19591 38651-38656 UBERON:0001977 denotes serum
T19592 38675-38681 NCBITaxon:9986 denotes rabbit
T19593 38687-38691 PR_EXT:000004182 denotes AQP2
T19594 38692-38700 GO:0042571 denotes antibody
T19595 38784-38798 CHEBI:51248 denotes AlexaFluor 594
T19596 38799-38809 MOP:0000779 denotes conjugated
T19597 38810-38817 NCBITaxon:9031 denotes chicken
T19598 38823-38829 NCBITaxon:9986 denotes rabbit
T19599 38830-38838 GO:0042571 denotes antibody
T19600 38847-38856 CHEBI_EXT:polyatomic_entity_or_group denotes Molecular
T19601 38857-38863 CHEBI_SO_EXT:molecular_probe denotes Probes
T19602 38897-38901 CHEBI:51231 denotes DAPI
T20367 39012-39017 CL_GO_EXT:cell denotes cells
T20368 39031-39035 CL_GO_EXT:cell denotes cell
T20369 39042-39052 GO:0010467 denotes expressing
T20370 39053-39059 SO_EXT:0000440 denotes vector
T20371 39067-39076 SO_EXT:wild_type_entity_or_quality denotes wild-type
T20372 39077-39081 PR_EXT:000004182 denotes AQP2
T20373 39086-39090 PR_EXT:000004182 denotes AQP2
T20374 39128-39138 GO:0010467 denotes expressing
T20375 39145-39154 SO_EXT:engineered_biological_sequence denotes construct
T20376 39161-39166 GO:0040007 denotes grown
T20377 39170-39181 PR_EXT:000007581 denotes fibronectin
T20378 39206-39221 GO_EXT:tight_junction denotes tight junctions
T20379 39230-39235 CL_GO_EXT:cell denotes Cells
T20380 39272-39281 CHEBI:42471 denotes forskolin
T20381 39307-39315 CHEBI:17790 denotes methanol
T20382 39341-39346 CL_GO_EXT:cell denotes cells
T20383 39385-39397 CHEBI:9750 denotes Triton X-100
T20384 39437-39441 PR_EXT:000004182 denotes AQP2
T20385 39446-39455 GO:0043226 denotes organelle
T20386 39456-39463 CHEBI_SO_EXT:molecular_indicator_or_label_or_marker_or_tag denotes markers
T20387 39479-39481 GO:0005886 denotes PM
T20388 39489-39491 GO:0005783 denotes ER
T20389 39493-39497 PR_EXT:000004182 denotes AQP2
T20390 39517-39521 NCBITaxon:9925 denotes goat
T20391 39527-39531 PR_EXT:000004182 denotes AQP2
T20392 39600-39614 CHEBI:52661 denotes AlexaFluor 488
T20393 39615-39625 MOP:0000779 denotes conjugated
T20394 39626-39632 NCBITaxon:9793 denotes donkey
T20395 39638-39642 NCBITaxon:9925 denotes goat
T20396 39653-39661 GO:0042571 denotes antibody
T20397 39667-39669 GO:0005886 denotes PM
T20398 39674-39676 GO:0005783 denotes ER
T20399 39695-39700 NCBITaxon:10088 denotes mouse
T20400 39706-39709 CHEBI:29101 denotes Na+
T20401 39706-39719 GO_EXT:0005391 denotes Na+/K+-ATPase
T20402 39710-39712 CHEBI:29103 denotes K+
T20403 39772-39778 NCBITaxon:9986 denotes rabbit
T20404 39784-39792 PR_EXT:000005005 denotes calnexin
T20405 39855-39865 GO:0042571 denotes antibodies
T20406 39884-39894 GO:0042571 denotes antibodies
T20407 39896-39899 CHEBI:37987 denotes Cy3
T20408 39900-39910 MOP:0000779 denotes conjugated
T20409 39911-39915 NCBITaxon:9925 denotes goat
T20410 39921-39926 NCBITaxon:10088 denotes mouse
T20411 40003-40017 CHEBI:51248 denotes AlexaFluor 594
T20412 40018-40028 MOP:0000779 denotes conjugated
T20413 40029-40036 NCBITaxon:9031 denotes chicken
T20414 40042-40048 NCBITaxon:9986 denotes rabbit
T20415 40071-40076 CL_GO_EXT:cell denotes Cells
T20416 40117-40121 CHEBI:51231 denotes DAPI
T20417 40179-40186 SO_EXT:sequence_fusion_entity_or_process denotes fusions
T20418 40198-40202 PR_EXT:000004182 denotes AQP2
T20419 40224-40232 GO:0042571 denotes antibody
T20420 40254-40260 UBERON:0002113 denotes kidney
T20421 40305-40309 PR_EXT:000004182 denotes AQP2
T20422 40352-40358 SO_EXT:sequence_fusion_entity_or_process denotes fusion
T20423 40359-40367 CHEBI_PR_EXT:protein denotes proteins
T20831 40726-40738 GO_PATO_EXT:biological_localization_process_or_quality denotes localization
T2391 5063-5069 GO:0006457 denotes folded
T8524 10504-10509 CHEBI:4450 denotes dDAVP
R247 T2329 T2328 _lexicallyChainedTo through,passage
R248 T2330 T2329 _lexicallyChainedTo membrane,through
R249 T2337 T2336 _lexicallyChainedTo in,water reabsorption
R250 T2338 T2337 _lexicallyChainedTo kidney,in
R251 T2346 T2345 _lexicallyChainedTo PM,regions of
R252 T2371 T2370 _lexicallyChainedTo cells,basolateral face of
R253 T2454 T2453 _lexicallyChainedTo cell,kidney
R910 T8572 T8571 _lexicallyChainedTo tubules,proximal
R912 T8444 T8443 _lexicallyChainedTo transversion,thymine to guanine
R913 T8444 T8447 _lexicallyChainedTo transversion,T to G
R914 T8713 T8712 _lexicallyChainedTo cells,surface of
R915 T8643 T8642 _lexicallyChainedTo cells,region of
R3377 T14052 T14051 _lexicallyChainedTo protein,transport of

craft-ca-core-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T490 22-26 NCBITaxon:10088 denotes Mice
T491 46-57 PR:000004182 denotes Aquaporin-2
T492 103-114 GO:0001822 denotes nephrogenic
T493 185-191 UBERON:0002113 denotes kidney
T494 207-212 UBERON:0001088 denotes urine
T495 241-246 NCBITaxon:9606 denotes Human
T496 261-272 GO:0001822 denotes nephrogenic
T497 335-357 PR:000001683 denotes vasopressin receptor 2
T498 359-364 PR:000001683 denotes Avpr2
T499 366-370 SO:0000704 denotes gene
T500 400-405 CHEBI:15377 denotes water
T501 414-425 PR:000004182 denotes aquaporin-2
T502 427-431 PR:000004182 denotes Aqp2
T503 433-437 SO:0000704 denotes gene
T504 516-522 NCBITaxon:33208 denotes animal
T505 546-553 SO:0000704 denotes genetic
T506 567-583 CHEBI:23995 denotes ethylnitrosourea
T507 596-600 NCBITaxon:10088 denotes mice
T508 657-662 NCBITaxon:10088 denotes mouse
T509 707-711 PR:000004182 denotes Aqp2
T510 712-716 SO:0000704 denotes gene
T511 746-752 NCBITaxon:39107 denotes murine
T512 772-776 NCBITaxon:10088 denotes mice
T513 788-797 UBERON:0000113 denotes adulthood
T514 832-837 NCBITaxon:9606 denotes human
T515 884-889 UBERON:0002113 denotes renal
T516 884-894 CL:1000497 denotes renal cell
T517 919-923 PR:000004182 denotes Aqp2
T518 979-984 CHEBI:15377 denotes water
T519 1003-1010 NCBITaxon:33208 denotes animals
T520 1064-1068 PR:000004182 denotes AQP2
T521 1110-1121 GO:0001822 denotes nephrogenic
T522 1155-1163 NCBITaxon:1 denotes organism
T523 1187-1192 UBERON:0002113 denotes renal
T524 1187-1197 CL:1000497 denotes renal cell
T525 1237-1241 PR:000004182 denotes AQP2
T526 1268-1289 GO:0005783 denotes endoplasmic reticulum
T527 1374-1379 NCBITaxon:10088 denotes mouse
T528 1447-1462 CHEBI:52217 denotes pharmacological
T529 1467-1471 SO:0000704 denotes gene
T2195 3091-3102 GO:0001822 denotes Nephrogenic
T2196 3168-3177 GO:0060073 denotes urination
T2197 3307-3308 GO:0000805 denotes X
T2198 3352-3357 PR:000001683 denotes Avpr2
T2199 3358-3362 SO:0000704 denotes gene
T2200 3371-3380 GO:0030849 denotes autosomal
T2201 3404-3408 PR:000004182 denotes Aqp2
T2202 3409-3413 SO:0000704 denotes gene
T2203 3419-3430 PR:000004182 denotes Aquaporin-2
T2204 3432-3436 PR:000004182 denotes AQP2
T2205 3489-3494 CHEBI:15377 denotes water
T2206 3519-3528 GO:0010467 denotes expressed
T2207 3532-3547 UBERON:0001232 denotes collecting-duct
T2208 3532-3563 CL:1001431 denotes collecting-duct principal cells
T2209 3571-3577 UBERON:0002113 denotes kidney
T2210 3619-3635 GO:0006833 denotes passage of water
T2211 3619-3626 _FRAGMENT denotes passage
T2212 3636-3643 _FRAGMENT denotes through
T2213 3655-3663 GO:0055085 denotes membrane
T2214 3630-3635 CHEBI:15377 denotes water
T2215 3648-3663 GO:0005886 denotes plasma membrane
T2216 3665-3667 GO:0005886 denotes PM
T2217 3747-3752 CHEBI:15377 denotes water
T2218 3747-3765 _FRAGMENT denotes water reabsorption
T2219 3777-3779 _FRAGMENT denotes in
T2220 3784-3790 GO:0070295 denotes kidney
T2221 3771-3776 UBERON:0001088 denotes urine
T2222 3784-3790 UBERON:0002113 denotes kidney
T2223 3867-3878 GO:0051262 denotes tetramerize
T2224 3911-3926 GO:0005886 denotes plasma membrane
T2225 4009-4019 _FRAGMENT denotes regions of
T2226 4024-4026 GO:0044459 denotes PM
T2227 4041-4045 PR:000004182 denotes AQP2
T2228 4063-4087 GO:0016324 denotes apical membrane of cells
T2229 4104-4119 UBERON:0001232 denotes collecting duct
T2230 4147-4151 PR:000004183 denotes AQP3
T2231 4180-4196 GO:1990794 denotes basolateral face
T2232 4231-4235 PR:000004182 denotes AQP2
T2233 4276-4291 GO:0005886 denotes plasma membrane
T2234 4343-4349 GO:0016324 denotes apical
T2235 4350-4363 GO:0005622 denotes intracellular
T2236 4364-4372 GO:0031982 denotes vesicles
T2237 4410-4415 CHEBI:15377 denotes water
T2238 4426-4430 PR:000004182 denotes AQP2
T2239 4451-4466 GO:0016324 denotes apical membrane
T2240 4479-4484 CHEBI:15377 denotes water
T2241 4556-4561 PR:000001683 denotes AVPR2
T2242 4570-4589 _FRAGMENT denotes basolateral face of
T2243 4610-4615 GO:1990794 denotes cells
T2244 4594-4609 UBERON:0001232 denotes collecting duct
T2245 4594-4615 CL:1001225 denotes collecting duct cells
T2246 4638-4651 GO:0005622 denotes intracellular
T2247 4652-4656 CHEBI:17489 denotes cAMP
T2248 4701-4705 PR:000004182 denotes AQP2
T2249 4723-4727 CHEBI:17489 denotes cAMP
T2250 4787-4802 GO:0005886 denotes plasma membrane
T2251 4822-4826 PR:000004182 denotes AQP2
T2252 4897-4901 PR:000004182 denotes Aqp2
T2253 4939-4945 NCBITaxon:9606 denotes humans
T2254 4964-4968 PR:000004182 denotes Aqp2
T2255 5063-5069 GO:0006457 denotes folded
T2256 5070-5075 CHEBI:15377 denotes water
T2257 5124-5128 CHEBI:17489 denotes cAMP
T2258 5260-5280 GO:0016323 denotes basolateral membrane
T2259 5297-5302 NCBITaxon:10088 denotes mouse
T2260 5391-5396 NCBITaxon:9606 denotes human
T2261 5402-5406 NCBITaxon:10088 denotes mice
T2262 5445-5449 PR:000004182 denotes Aqp2
T2263 5454-5459 PR:000001683 denotes Avpr2
T2264 5499-5504 NCBITaxon:10088 denotes mouse
T2265 5543-5547 PR:000004182 denotes Aqp2
T2266 5548-5552 SO:0000704 denotes gene
T2267 5586-5590 NCBITaxon:10088 denotes mice
T2268 5591-5595 GO:0016265 denotes died
T2269 5613-5618 GO:0007567 denotes birth
T2270 5635-5640 PR:000001683 denotes AVPR2
T2271 5666-5669 GO:0016265 denotes die
T2272 5698-5703 GO:0007567 denotes birth
T2273 5744-5749 NCBITaxon:10088 denotes mouse
T2274 5776-5784 NCBITaxon:1 denotes organism
T2275 5800-5805 CHEBI:15377 denotes water
T2276 5800-5817 GO:0030104 denotes water homeostasis
T2277 5872-5879 SO:0000704 denotes genetic
T2278 5890-5895 NCBITaxon:10088 denotes mouse
T2279 5904-5908 PR:000004182 denotes Aqp2
T2280 5985-5991 NCBITaxon:39107 denotes murine
T2281 6011-6022 GO:0001822 denotes nephrogenic
T2282 6071-6075 PR:000004182 denotes Aqp2
T2283 6076-6080 SO:0000704 denotes gene
T2284 6087-6093 SO:0001023 denotes allele
T2285 6097-6101 PR:000004182 denotes Aqp2
T2286 6131-6136 NCBITaxon:10088 denotes mouse
T2287 6184-6188 UBERON:0000104 denotes life
T2288 6231-6235 PR:000004182 denotes AQP2
T2289 6283-6304 UBERON:0001232 denotes renal collecting-duct
T2290 6283-6310 CL:1001225 denotes renal collecting-duct cells
T2291 6358-6370 CHEBI:4450 denotes desmopressin
T2292 6430-6436 NCBITaxon:9608 denotes canine
T2293 6437-6443 UBERON:0002113 denotes kidney
T2294 6437-6443 _FRAGMENT denotes kidney
T2295 6451-6455 CL:1000497 denotes cell
T2296 6477-6498 GO:0005783 denotes endoplasmic reticulum
T2297 6608-6617 GO:0030849 denotes autosomal
T2298 6661-6665 PR:000004182 denotes AQP2
T2299 6688-6694 NCBITaxon:40674 denotes mammal
T8093 6743-6759 CHEBI:23995 denotes ethylnitrosourea
T8094 6761-6764 CHEBI:23995 denotes ENU
T8095 6799-6805 NCBITaxon:33208 denotes animal
T8096 6865-6875 GO:0008152 denotes metabolism
T8097 6906-6910 NCBITaxon:10088 denotes mice
T8098 6916-6924 GO:0060073 denotes urinated
T8099 6929-6934 GO:0042756 denotes drank
T8100 6948-6953 UBERON:0001977 denotes Serum
T8101 6958-6963 UBERON:0001088 denotes urine
T8102 6985-6991 UBERON:0001969 denotes plasma
T8103 6992-6999 CHEBI:17234 denotes glucose
T8104 7036-7043 CHEBI:17234 denotes glucose
T8105 7051-7056 UBERON:0001088 denotes urine
T8106 7149-7153 NCBITaxon:10088 denotes mice
T8107 7174-7179 SO:0000704 denotes genic
T8108 7181-7190 GO:0030849 denotes autosomal
T8109 7216-7220 PR:000004182 denotes Aqp2
T8110 7233-7237 SO:0000704 denotes gene
T8111 7253-7257 PR:000004182 denotes Aqp2
T8112 7284-7288 NCBITaxon:10088 denotes mice
T8113 7302-7320 _FRAGMENT denotes thymine to guanine
T8114 7330-7342 SO:1000022 denotes transversion
T8115 7322-7328 _FRAGMENT denotes T to G
T8116 8492-8496 PR:000004182 denotes AQP2
T8117 8511-8519 GO:0016020 denotes membrane
T8118 8520-8525 CHEBI:15377 denotes water
T8119 8558-8571 GO:0005576 denotes extracellular
T8120 8590-8598 GO:0016020 denotes membrane
T8121 8608-8614 SO:0000417 denotes domain
T8122 8677-8685 GO:0016020 denotes membrane
T8123 8695-8702 SO:0000417 denotes domains
T8124 8723-8733 NCBITaxon:7742 denotes vertebrate
T8125 8734-8741 NCBITaxon:species denotes species
T8126 8836-8846 NCBITaxon:7742 denotes vertebrate
T8127 8847-8851 PR:000004182 denotes AQP2
T8128 8908-8912 PR:000004182 denotes Aqp2
T8129 8924-8928 NCBITaxon:10088 denotes mice
T8130 8957-8962 UBERON:0001088 denotes urine
T8131 9012-9017 UBERON:0001088 denotes urine
T8132 9128-9133 CHEBI:15377 denotes water
T8133 9205-9210 CHEBI:15377 denotes water
T8134 9211-9217 GO:0007631 denotes intake
T8135 9234-9238 NCBITaxon:10088 denotes mice
T8136 9272-9277 CHEBI:15377 denotes water
T8137 9278-9284 GO:0007631 denotes intake
T8138 9379-9386 UBERON:0001088 denotes urinary
T8139 9398-9403 CHEBI:15377 denotes water
T8140 9404-9410 GO:0007631 denotes intake
T8141 9489-9496 NCBITaxon:33208 denotes animals
T8142 9570-9575 UBERON:0001088 denotes urine
T8143 9646-9650 PR:000004182 denotes Aqp2
T8144 9662-9666 NCBITaxon:10088 denotes mice
T8145 9687-9692 UBERON:0001088 denotes urine
T8146 9712-9717 UBERON:0001088 denotes urine
T8147 9742-9746 NCBITaxon:10088 denotes mice
T8148 9808-9812 NCBITaxon:10088 denotes mice
T8149 9836-9841 UBERON:0001088 denotes urine
T8150 9869-9876 GO:0065007 denotes control
T8151 9884-9896 UBERON:0001898 denotes hypothalamus
T8152 9955-9963 GO:0046903 denotes secretes
T8153 9995-10029 CHEBI:4450 denotes 1-deamino-8-D-arginine vasopressin
T8154 10031-10036 CHEBI:4450 denotes dDAVP
T8155 10050-10062 CHEBI:4450 denotes desmopressin
T8156 10088-10093 PR:000001683 denotes AVPR2
T8157 10126-10130 NCBITaxon:10088 denotes mice
T8158 10132-10137 CHEBI:4450 denotes dDAVP
T8159 10170-10175 UBERON:0001088 denotes urine
T8160 10270-10274 NCBITaxon:10088 denotes mice
T8161 10293-10298 UBERON:0001088 denotes urine
T8162 10396-10403 NCBITaxon:33208 denotes animals
T8163 10445-10450 UBERON:0001088 denotes urine
T8164 10504-10509 CHEBI:4450 denotes dDAVP
T8165 10535-10540 CHEBI:4450 denotes dDAVP
T8166 10588-10592 PR:000004182 denotes AQP2
T8167 10652-10662 NCBITaxon:1 denotes individual
T8168 10698-10703 NCBITaxon:10088 denotes mouse
T8169 10732-10739 GO:0007618 denotes matings
T8170 10752-10759 NCBITaxon:33208 denotes animals
T8171 10993-10998 UBERON:0001088 denotes urine
T8172 11014-11019 CHEBI:15377 denotes water
T8173 11020-11026 GO:0007631 denotes intake
T8174 11067-11071 NCBITaxon:10088 denotes mice
T8175 11088-11095 UBERON:0002113 denotes kidneys
T8176 11130-11137 NCBITaxon:33208 denotes animals
T8177 11194-11198 NCBITaxon:10088 denotes mice
T8178 11221-11229 UBERON:0000104 denotes lifespan
T8179 11239-11245 NCBITaxon:33208 denotes animal
T8180 11292-11299 NCBITaxon:33208 denotes animals
T8181 12159-12163 PR:000004182 denotes Aqp2
T8182 12175-12179 NCBITaxon:10088 denotes mice
T8183 12332-12339 UBERON:0002113 denotes kidneys
T8184 12347-12351 PR:000004182 denotes Aqp2
T8185 12363-12367 NCBITaxon:10088 denotes mice
T8186 12427-12431 NCBITaxon:10088 denotes mice
T8187 12516-12520 PR:000004177 denotes Aqp1
T8188 12521-12525 PR:000004183 denotes Aqp3
T8189 12536-12540 NCBITaxon:10088 denotes mice
T8190 12582-12586 PR:000004182 denotes Aqp2
T8191 12598-12602 NCBITaxon:10088 denotes mice
T8192 12648-12652 PR:000004182 denotes Aqp2
T8193 12664-12668 NCBITaxon:10088 denotes mice
T8194 12707-12719 UBERON:0001224 denotes renal pelvis
T8195 12749-12755 UBERON:0000056 denotes ureter
T8196 12795-12813 UBERON:0006660 denotes muscularis propria
T8197 12902-12912 UBERON:0000365 denotes urothelium
T8198 12923-12946 UBERON:0005910 denotes transitional epithelium
T8199 12998-13004 UBERON:0002113 denotes kidney
T8200 13022-13035 UBERON:0002015 denotes renal capsule
T8201 13039-13051 UBERON:0001224 denotes renal pelvis
T8202 13094-13103 UBERON:0000074 denotes glomeruli
T8203 13108-13116 _FRAGMENT denotes proximal
T8204 13124-13131 UBERON:0004134 denotes tubules
T8205 13117-13131 UBERON:0004135 denotes distal tubules
T8206 13241-13245 PR:000004182 denotes AQP2
T8207 13542-13549 CHEBI:37684 denotes mannose
T8208 13567-13571 PR:000004182 denotes AQP2
T8209 13654-13661 UBERON:0002113 denotes kidneys
T8210 13675-13682 NCBITaxon:33208 denotes animals
T8211 13684-13688 PR:000004182 denotes AQP2
T8212 13701-13708 NCBITaxon:33208 denotes animals
T8213 13888-13895 NCBITaxon:33208 denotes animals
T8214 14052-14059 MOP:0000780 denotes cleaves
T8215 14060-14067 CHEBI:37684 denotes mannose
T8216 14073-14085 CHEBI:16646 denotes carbohydrate
T8217 14137-14141 PR:000004182 denotes AQP2
T8218 14147-14154 UBERON:0002113 denotes kidneys
T8219 14194-14201 NCBITaxon:33208 denotes animals
T8220 14341-14345 PR:000004182 denotes Aqp2
T8221 14357-14361 NCBITaxon:10088 denotes mice
T8222 14408-14412 PR:000004182 denotes Aqp2
T8223 14424-14428 NCBITaxon:10088 denotes mice
T8224 14478-14483 CHEBI:4450 denotes dDAVP
T8225 14989-14995 NCBITaxon:9606 denotes humans
T8226 15007-15014 SO:0001023 denotes alleles
T8227 15018-15022 PR:000004182 denotes Aqp2
T8228 15099-15105 GO:0045177 denotes apical
T8229 15106-15118 GO:0009986 denotes cell surface
T8230 15205-15209 PR:000004182 denotes Aqp2
T8231 15233-15238 NCBITaxon:9606 denotes human
T8232 15261-15272 GO:0009294 denotes transfected
T8233 15278-15284 UBERON:0002113 denotes kidney
T8234 15278-15289 CL:1000497 denotes kidney cell
T8235 15324-15331 SO:0001023 denotes alleles
T8236 15406-15414 GO:0031982 denotes vesicles
T8237 15448-15469 GO:0005783 denotes endoplasmic reticulum
T8238 15471-15473 GO:0005783 denotes ER
T8239 15480-15485 NCBITaxon:10088 denotes mouse
T8240 15565-15571 NCBITaxon:33208 denotes animal
T8241 15626-15630 PR:000004182 denotes AQP2
T8242 15674-15680 GO:0016324 denotes apical
T8243 15681-15690 _FRAGMENT denotes region of
T8244 15707-15712 GO:0044464 denotes cells
T8245 15691-15706 UBERON:0001232 denotes collecting duct
T8246 15691-15712 CL:1001225 denotes collecting duct cells
T8247 15716-15723 UBERON:0002113 denotes kidneys
T8248 15737-15741 NCBITaxon:10088 denotes mice
T8249 15765-15770 CHEBI:4450 denotes dDAVP
T8250 15772-15776 PR:000004182 denotes AQP2
T8251 15805-15817 GO:0009986 denotes cell surface
T8252 15846-15853 UBERON:0002113 denotes kidneys
T8253 15872-15879 NCBITaxon:33208 denotes animals
T8254 15890-15894 PR:000004182 denotes AQP2
T8255 15989-15993 PR:000004183 denotes AQP3
T8256 16033-16044 GO:1990794 denotes basolateral
T8257 16045-16052 GO:0009986 denotes surface
T8258 16077-16082 CHEBI:4450 denotes dDAVP
T8259 16096-16100 PR:000004182 denotes AQP2
T8260 16136-16148 GO:0009986 denotes cell surface
T8261 16230-16237 UBERON:0002113 denotes kidneys
T8262 16261-16265 NCBITaxon:10088 denotes mice
T8263 16319-16324 CHEBI:4450 denotes dDAVP
T8264 18087-18091 PR:000004182 denotes AQP2
T8265 18112-18124 GO:0009294 denotes transfection
T8266 18158-18168 GO:0010467 denotes expressing
T8267 18169-18174 NCBITaxon:10088 denotes mouse
T8268 18185-18189 PR:000004182 denotes AQP2
T8269 18194-18198 PR:000004182 denotes AQP2
T8270 18354-18358 NCBITaxon:10088 denotes mice
T8271 18451-18461 GO:0010467 denotes expressing
T8272 18462-18466 PR:000004182 denotes AQP2
T8273 18558-18569 GO:0009294 denotes transfected
T8274 18606-18610 PR:000004182 denotes AQP2
T8275 18688-18694 GO:0016324 denotes apical
T8276 18761-18770 GO:0031982 denotes vesicular
T8277 18793-18797 PR:000004182 denotes AQP2
T8278 18855-18862 GO:0005634 denotes nuclear
T8279 18920-18929 CHEBI:42471 denotes forskolin
T8280 18933-18937 CHEBI:17489 denotes cAMP
T8281 18984-18988 PR:000004182 denotes AQP2
T8282 19009-19015 GO:0045177 denotes apical
T8283 19016-19026 _FRAGMENT denotes surface of
T8284 19042-19047 GO:0009986 denotes cells
T8285 19102-19109 GO:0005634 denotes nuclear
T8286 19126-19130 PR:000004182 denotes AQP2
T8287 19195-19204 CHEBI:42471 denotes forskolin
T8288 19257-19264 GO:0005634 denotes nuclear
T8289 19281-19285 PR:000004182 denotes AQP2
T8290 19314-19316 GO:0005783 denotes ER
T8291 19361-19365 PR:000004182 denotes AQP2
T8292 19389-19391 GO:0005783 denotes ER
T8293 19413-19424 GO:0009294 denotes transfected
T8294 19430-19434 PR:000004182 denotes Aqp2
T8295 19451-19455 PR:000004182 denotes AQP2
T8296 19463-19465 GO:0005783 denotes ER
T8297 19474-19482 PR:000005005 denotes calnexin
T8298 19514-19522 PR:000005005 denotes calnexin
T8299 19528-19532 PR:000004182 denotes AQP2
T8300 19593-19597 PR:000004182 denotes AQP2
T8301 19629-19637 PR:000005005 denotes calnexin
T8302 19690-19692 GO:0005783 denotes ER
T8303 19707-19711 PR:000004182 denotes AQP2
T8304 19761-19763 GO:0005783 denotes ER
T8305 19771-19773 GO:0005783 denotes ER
T8306 19856-19860 PR:000004182 denotes AQP2
T8307 19877-19884 UBERON:0002113 denotes kidneys
T8308 19902-19909 NCBITaxon:33208 denotes Animals
T8309 19931-19935 PR:000004182 denotes Aqp2
T8310 19977-19982 UBERON:0001088 denotes urine
T8311 19997-20002 UBERON:0001088 denotes urine
T8312 20082-20088 SO:0001023 denotes allele
T8313 20090-20094 PR:000004182 denotes AQP2
T8314 20146-20153 CL:0000023 denotes oocytes
T8315 20172-20188 GO:0051260 denotes homo-oligomerize
T8316 20220-20227 UBERON:0002113 denotes kidneys
T8317 20246-20253 NCBITaxon:33208 denotes animals
T8318 20303-20307 PR:000004182 denotes AQP2
T8319 20363-20386 UBERON:0001232 denotes kidney collecting ducts
T8320 20392-20396 PR:000004182 denotes Aqp2
T8321 20404-20408 NCBITaxon:10088 denotes mice
T8322 20473-20477 PR:000004182 denotes AQP2
T8323 20509-20515 GO:0045177 denotes apical
T8324 20516-20528 GO:0009986 denotes cell surface
T8325 20534-20539 CHEBI:4450 denotes dDAVP
T8326 20925-20929 PR:000004182 denotes AQP2
T8327 20937-20949 GO:0009986 denotes cell surface
T8328 21019-21023 PR:000004182 denotes AQP2
T8329 21085-21089 PR:000004182 denotes AQP2
T8330 21116-21125 GO:0010467 denotes expressed
T8331 22763-22779 UBERON:0001232 denotes collecting ducts
T8332 22785-22789 PR:000004182 denotes Aqp2
T8333 22797-22801 NCBITaxon:10088 denotes mice
T8334 22895-22899 PR:000004182 denotes AQP2
T8335 23004-23015 GO:0009294 denotes transfected
T8336 23058-23068 GO:0010467 denotes expressing
T8337 23079-23083 PR:000004182 denotes AQP2
T8338 23101-23112 GO:0009294 denotes transfected
T8339 23122-23132 GO:0010467 denotes expression
T8340 23174-23178 PR:000004182 denotes AQP2
T8341 23180-23184 PR:000004182 denotes AQP2
T8342 23206-23216 GO:0042571 denotes Antibodies
T8343 23260-23264 PR:000004182 denotes AQP2
T8344 23270-23274 PR:000004182 denotes AQP2
T8345 23282-23286 PR:000004182 denotes AQP2
T8346 23313-23324 GO:0009294 denotes transfected
T8347 23369-23380 GO:0009294 denotes transfected
T8348 23404-23414 GO:0010467 denotes expressing
T8349 23425-23429 PR:000004182 denotes AQP2
T8350 23478-23487 GO:0016020 denotes membranes
T8351 23510-23514 PR:000004182 denotes AQP2
T8352 23531-23540 GO:0010467 denotes expressed
T8353 23783-23794 GO:0009294 denotes transfected
T8354 23810-23814 PR:000004182 denotes AQP2
T8355 23815-23825 GO:0010467 denotes expressing
T8356 23826-23832 SO:0000440 denotes vector
T8357 23847-23853 SO:0000440 denotes vector
T8358 23887-23898 GO:0009294 denotes transfected
T8359 23899-23903 PR:000004182 denotes AQP2
T8360 23911-23915 PR:000004182 denotes AQP2
T8361 23926-23936 GO:0010467 denotes expression
T8362 23949-23953 PR:000004182 denotes AQP2
T8363 23975-23981 GO:0045177 denotes apical
T8364 23982-23989 GO:0009986 denotes surface
T8365 23995-24004 CHEBI:42471 denotes forskolin
T8366 24044-24055 GO:0009294 denotes transfected
T8367 24061-24067 SO:0000440 denotes vector
T8368 24128-24132 PR:000004182 denotes AQP2
T8369 24165-24169 PR:000004182 denotes AQP2
T8370 24186-24195 GO:0010467 denotes expressed
T8371 24209-24221 GO:0009294 denotes transfection
T8372 24227-24233 SO:0000440 denotes vector
T8373 24263-24274 GO:0005737 denotes cytoplasmic
T8374 24326-24335 GO:0010467 denotes expressed
T8375 24349-24353 PR:000004182 denotes AQP2
T8376 24370-24374 PR:000004182 denotes AQP2
T8377 24402-24408 GO:0045177 denotes apical
T8378 24409-24416 GO:0009986 denotes surface
T8379 24545-24557 GO:0009294 denotes transfection
T8380 24577-24588 GO:0009294 denotes transfected
T8381 24636-24646 GO:0010467 denotes expressing
T8382 24657-24661 PR:000004182 denotes AQP2
T8383 24672-24678 GO:0045177 denotes apical
T8384 24684-24693 CHEBI:42471 denotes forskolin
T8385 24725-24735 GO:0010467 denotes expression
T8386 24765-24769 PR:000004182 denotes AQP2
T8387 24788-24792 PR:000004182 denotes AQP2
T8388 24844-24848 PR:000004182 denotes AQP2
T8389 24876-24880 PR:000004182 denotes AQP2
T8390 24902-24908 GO:0045177 denotes apical
T8391 24914-24923 CHEBI:42471 denotes forskolin
T8392 24983-24987 PR:000004182 denotes AQP2
T8393 25026-25032 GO:0045177 denotes apical
T8394 25033-25040 GO:0009986 denotes surface
T8395 25073-25077 PR:000004182 denotes AQP2
T8396 25105-25111 GO:0045177 denotes apical
T8397 25127-25131 PR:000004182 denotes AQP2
T8398 25141-25150 GO:0010467 denotes expressed
T8399 25195-25206 GO:0009294 denotes transfected
T8400 25238-25242 PR:000004182 denotes AQP2
T8401 25246-25252 SO:0000440 denotes vector
T8402 25270-25282 MOP:0000093 denotes biotinylated
T8403 25283-25290 GO:0009986 denotes surface
T8404 25306-25315 CHEBI:42471 denotes forskolin
T8405 25350-25362 MOP:0000093 denotes biotinylated
T8406 25385-25389 PR:000004182 denotes AQP2
T8407 25403-25412 GO:0010467 denotes expressed
T8408 25487-25499 MOP:0000093 denotes biotinylated
T8409 25520-25524 PR:000004182 denotes AQP2
T8410 25533-25542 GO:0010467 denotes expressed
T8411 25555-25562 GO:0009986 denotes surface
T8412 25563-25575 MOP:0000093 denotes biotinylated
T8413 25589-25601 GO:0009986 denotes cell surface
T8414 25629-25635 CHEBI:15956 denotes biotin
T8415 25665-25669 PR:000004182 denotes AQP2
T8416 25698-25710 GO:0009986 denotes cell surface
T8417 25726-25730 PR:000004182 denotes AQP2
T13804 25775-25779 PR:000004182 denotes Aqp2
T13805 25790-25794 NCBITaxon:10088 denotes mice
T13806 25810-25814 GO:0040007 denotes grow
T13807 25819-25828 GO:0000003 denotes reproduce
T13808 25909-25914 UBERON:0001088 denotes urine
T13809 25937-25942 UBERON:0001088 denotes urine
T13810 25954-25959 CHEBI:15377 denotes water
T13811 25960-25966 GO:0007631 denotes intake
T13812 25995-26000 NCBITaxon:10088 denotes mouse
T13813 26041-26047 NCBITaxon:9606 denotes humans
T13814 26079-26084 PR:000001683 denotes Avpr2
T13815 26088-26092 PR:000004182 denotes Aqp2
T13816 26110-26111 GO:0000805 denotes X
T13817 26119-26124 PR:000001683 denotes Avpr2
T13818 26125-26129 SO:0000704 denotes gene
T13819 26133-26137 NCBITaxon:10088 denotes mice
T13820 26243-26249 UBERON:0007023 denotes adults
T13821 26257-26261 NCBITaxon:10088 denotes mice
T13822 26262-26266 GO:0016265 denotes died
T13823 26282-26287 GO:0007567 denotes birth
T13824 26293-26298 UBERON:0007023 denotes adult
T13825 26360-26367 UBERON:0001088 denotes urinary
T13826 26379-26384 CHEBI:15377 denotes water
T13827 26385-26391 GO:0007631 denotes intake
T13828 26406-26411 UBERON:0001088 denotes urine
T13829 26440-26445 NCBITaxon:10088 denotes mouse
T13830 26446-26450 PR:000004182 denotes Aqp2
T13831 26451-26455 SO:0000704 denotes gene
T13832 26495-26500 NCBITaxon:9606 denotes human
T13833 26570-26574 NCBITaxon:10088 denotes mice
T13834 26589-26594 UBERON:0001088 denotes urine
T13835 26645-26650 GO:0016265 denotes death
T13836 26666-26671 GO:0007567 denotes birth
T13837 26684-26688 PR:000004182 denotes AQP2
T13838 26770-26785 UBERON:0001232 denotes collecting duct
T13839 26868-26872 NCBITaxon:10088 denotes mice
T13840 26912-26916 PR:000004182 denotes Aqp2
T13841 26933-26937 SO:0000704 denotes gene
T13842 26962-26966 NCBITaxon:10088 denotes mice
T13843 26993-26997 PR:000004182 denotes AQP2
T13844 27028-27033 CHEBI:15377 denotes water
T13845 27028-27046 GO:0006833 denotes water transporting
T13846 27102-27107 CHEBI:15377 denotes water
T13847 27143-27147 PR:000004182 denotes AQP2
T13848 27175-27182 NCBITaxon:33208 denotes animals
T13849 27211-27216 CHEBI:4450 denotes dDAVP
T13850 27227-27232 CHEBI:4450 denotes dDAVP
T13851 27244-27249 UBERON:0001088 denotes urine
T13852 27298-27304 UBERON:0002113 denotes kidney
T13853 27316-27320 PR:000004182 denotes AQP2
T13854 27358-27363 CHEBI:15377 denotes water
T13855 27401-27407 GO:0045177 denotes apical
T13856 27408-27420 GO:0009986 denotes cell surface
T13857 27427-27432 CHEBI:4450 denotes dDAVP
T13858 27470-27474 PR:000004182 denotes AQP2
T13859 27565-27577 GO:0009986 denotes cell surface
T13860 27583-27590 GO:0009986 denotes surface
T13861 27591-27604 MOP:0000093 denotes biotinylation
T13862 27672-27679 GO:0009986 denotes surface
T13863 27897-27899 GO:0005783 denotes ER
T13864 27996-28003 CHEBI:37684 denotes mannose
T13865 28261-28263 GO:0005783 denotes ER
T13866 28320-28324 PR:000004182 denotes AQP2
T13867 28340-28342 GO:0005783 denotes ER
T13868 28351-28359 PR:000005005 denotes calnexin
T13869 28363-28374 GO:0009294 denotes transfected
T13870 28449-28451 GO:0005783 denotes ER
T13871 28483-28485 GO:0005783 denotes ER
T13872 28510-28515 CHEBI:4450 denotes dDAVP
T13873 28579-28586 NCBITaxon:33208 denotes animals
T13874 28596-28608 _FRAGMENT denotes transport of
T13875 28630-28637 GO:0015031 denotes protein
T13876 28649-28651 GO:0005783 denotes ER
T13877 28706-28710 PR:000004182 denotes AQP2
T13878 28720-28727 GO:0006457 denotes folding
T13879 28776-28781 CHEBI:15377 denotes water
T13880 28776-28794 GO:0006833 denotes water transporting
T13881 28891-28898 NCBITaxon:33208 denotes animals
T13882 28934-28936 GO:0005783 denotes ER
T13883 28953-28957 PR:000004182 denotes AQP2
T13884 28995-29002 CHEBI:37684 denotes mannose
T13885 29031-29038 CHEBI:37684 denotes mannose
T13886 29044-29059 CHEBI:50699 denotes oligosaccharide
T13887 29076-29078 GO:0005783 denotes ER
T13888 29123-29138 GO:0005794 denotes Golgi apparatus
T13889 29170-29177 CHEBI:37684 denotes mannose
T13890 29199-29203 PR:000004182 denotes AQP2
T13891 29259-29261 GO:0005783 denotes ER
T13892 29278-29292 CHEBI:50699 denotes oligosaccharyl
T13893 29337-29341 PR:000004182 denotes AQP2
T13894 29386-29390 NCBITaxon:10088 denotes mice
T13895 29441-29445 NCBITaxon:10088 denotes mice
T13896 29506-29510 NCBITaxon:10088 denotes mice
T13897 29612-29619 UBERON:0002113 denotes kidneys
T13898 29767-29769 GO:0005783 denotes ER
T13899 29824-29828 PR:000004182 denotes Aqp2
T13900 29836-29843 NCBITaxon:33208 denotes animals
T13901 29987-29993 SO:0001023 denotes allele
T13902 29997-30001 PR:000004182 denotes Aqp2
T13903 30045-30054 GO:0010467 denotes expressed
T13904 30197-30201 PR:000004182 denotes Aqp2
T13905 30209-30213 NCBITaxon:10088 denotes mice
T13906 30235-30239 PR:000004182 denotes AQP2
T13907 30274-30278 PR:000004182 denotes AQP2
T13908 30303-30312 GO:0010467 denotes expressed
T13909 30337-30341 PR:000004182 denotes AQP2
T13910 30362-30374 GO:0009986 denotes cell surface
T13911 30465-30471 SO:0001023 denotes allele
T13912 30482-30486 PR:000004182 denotes AQP2
T13913 30534-30538 PR:000004182 denotes AQP2
T13914 30562-30566 PR:000004182 denotes AQP2
T13915 30681-30687 SO:0001023 denotes allele
T13916 30735-30742 UBERON:0002113 denotes kidneys
T13917 30757-30761 PR:000004182 denotes Aqp2
T13918 30773-30777 NCBITaxon:10088 denotes mice
T13919 30800-30810 GO:0010467 denotes expressing
T13920 30811-30826 UBERON:0001232 denotes collecting duct
T13921 30811-30832 CL:1001225 denotes collecting duct cells
T13922 30849-30854 CHEBI:15377 denotes water
T13923 30905-30927 GO:0016324 denotes apical plasma membrane
T13924 30943-30948 CHEBI:4450 denotes dDAVP
T13925 31061-31065 PR:000004182 denotes Aqp2
T13926 31077-31089 GO:0009294 denotes Transfection
T13927 31124-31128 PR:000004182 denotes Aqp2
T13928 31166-31171 NCBITaxon:9606 denotes human
T13929 31172-31179 SO:0001023 denotes alleles
T13930 31279-31294 GO:0005886 denotes plasma membrane
T13931 31305-31312 GO:0006457 denotes folding
T13932 31331-31333 GO:0005783 denotes ER
T13933 31377-31382 CHEBI:4450 denotes dDAVP
T13934 31418-31427 GO:0030849 denotes autosomal
T13935 31520-31525 NCBITaxon:9606 denotes human
T13936 31536-31541 NCBITaxon:10088 denotes mouse
T13937 31567-31571 PR:000004182 denotes Aqp2
T13938 31572-31578 SO:0001023 denotes allele
T13939 31653-31657 SO:0000704 denotes gene
T15491 31755-31758 CHEBI:23995 denotes ENU
T15492 31759-31763 NCBITaxon:10088 denotes mice
T15493 31777-31780 CHEBI:23995 denotes ENU
T15494 31801-31805 NCBITaxon:10088 denotes mice
T15495 31840-31844 NCBITaxon:10088 denotes Mice
T15496 31886-31893 NCBITaxon:33208 denotes animals
T15497 32001-32007 NCBITaxon:33208 denotes animal
T15498 32161-32167 NCBITaxon:33208 denotes Animal
T15862 32237-32242 NCBITaxon:10088 denotes mouse
T15863 32243-32247 PR:000004182 denotes AQP2
T15864 32302-32309 SO:0000155 denotes plasmid
T15865 32334-32341 GO:0006266 denotes ligated
T15866 32596-32605 SO:0000077 denotes antisense
T15867 32688-32692 PR:000004182 denotes AQP2
T15868 32710-32714 PR:000004182 denotes AQP2
T15869 32761-32768 SO:0000112 denotes primers
T15870 32830-32840 SO:0000319 denotes stop codon
T15871 32844-32848 PR:000004182 denotes AQP2
T15872 32889-32894 PR:P23940 denotes BamHI
T15873 32899-32906 GO:0006266 denotes ligated
T15874 33026-33035 CHEBI:25435 denotes mutagenic
T16369 33295-33305 CHEBI:17334 denotes penicillin
T16370 33324-33336 CHEBI:17076 denotes streptomycin
T16371 33352-33355 CHEBI:16526 denotes CO2
T16372 33404-33415 GO:0009294 denotes transfected
T16373 33471-33481 GO:0010467 denotes expression
T16374 33515-33519 PR:000004182 denotes AQP2
T16375 33521-33525 PR:000004182 denotes AQP2
T16376 33539-33545 SO:0000667 denotes insert
T16377 33575-33579 CHEBI:42768 denotes G418
T16378 33686-33696 CHEBI:33282 denotes antibiotic
T16379 33747-33760 GO:0009294 denotes transfections
T16659 33859-33863 PR:000004182 denotes Aqp2
T16660 33882-33886 NCBITaxon:10088 denotes mice
T16661 33892-33897 SO:0000147 denotes exons
T16662 33901-33905 PR:000004182 denotes Aqp2
T16663 33926-33931 NCBITaxon:10088 denotes mouse
T16664 33932-33939 SO:0001026 denotes genomic
T16665 33975-33979 SO:0000147 denotes exon
T16666 34006-34013 SO:0000112 denotes primers
T16933 34074-34079 UBERON:0001088 denotes Urine
T16934 34100-34105 UBERON:0001088 denotes urine
T16935 34148-34153 UBERON:0007023 denotes adult
T16936 34154-34158 NCBITaxon:10088 denotes mice
T16937 34170-34179 GO:0008152 denotes Metabolic
T16938 34253-34258 UBERON:0001088 denotes urine
T16939 34278-34283 UBERON:0001088 denotes Urine
T16940 34405-34410 UBERON:0001088 denotes Urine
T16941 34462-34472 UBERON:0001179 denotes peritoneal
T16942 34486-34491 CHEBI:4450 denotes dDAVP
T16943 34505-34509 NCBITaxon:10088 denotes Mice
T16944 34535-34540 CHEBI:4450 denotes dDAVP
T16945 34578-34583 UBERON:0001088 denotes Urine
T17247 34669-34675 UBERON:0002113 denotes Kidney
T17248 34676-34684 GO:0016020 denotes membrane
T17249 34704-34709 NCBITaxon:10088 denotes mouse
T17250 34710-34717 UBERON:0002113 denotes kidneys
T17251 34744-34748 CHEBI:9754 denotes Tris
T17252 34766-34773 CHEBI:17992 denotes sucrose
T17253 35028-35037 GO:0016020 denotes membranes
T17578 35152-35158 UBERON:0002113 denotes Kidney
T17579 35159-35167 GO:0016020 denotes membrane
T17580 35209-35212 CHEBI:8984 denotes SDS
T17581 35253-35267 CHEBI:53325 denotes nitrocellulose
T17582 35278-35287 GO:0016020 denotes Membranes
T17583 35322-35326 CHEBI:9754 denotes Tris
T17584 35354-35362 CHEBI:53424 denotes Tween 20
T17585 35422-35426 PR:000004182 denotes AQP2
T17586 35438-35446 GO:0042571 denotes antibody
T17587 35524-35533 GO:0016020 denotes Membranes
T17588 35578-35588 MOP:0000779 denotes conjugated
T17589 35589-35595 NCBITaxon:9793 denotes donkey
T17590 35601-35605 NCBITaxon:9925 denotes goat
T17591 35606-35614 GO:0042571 denotes antibody
T17592 35616-35625 GO:0016020 denotes Membranes
T17593 35690-35697 CHEBI:33893 denotes reagent
T17892 35783-35789 UBERON:0002113 denotes Kidney
T17893 35790-35799 GO:0016020 denotes membranes
T17894 35832-35848 CHEBI:37586 denotes sodium phosphate
T17895 35864-35867 CHEBI:8984 denotes SDS
T17896 35879-35896 CHEBI:41218 denotes β-mercaptoethanol
T17897 36100-36109 CHEBI:33893 denotes reactants
T18690 36176-36189 MOP:0000093 denotes biotinylation
T18691 36223-36233 GO:0010467 denotes expressing
T18692 36244-36248 PR:000004182 denotes AQP2
T18693 36250-36255 GO:0040007 denotes grown
T18694 36278-36289 GO:0009294 denotes transfected
T18695 36311-36315 PR:000004182 denotes AQP2
T18696 36323-36327 PR:000004182 denotes AQP2
T18697 36338-36344 SO:0000440 denotes vector
T18698 36388-36392 CHEBI:9754 denotes Tris
T18699 36425-36432 CHEBI:17992 denotes sucrose
T18700 36521-36530 GO:0016020 denotes membranes
T18701 36585-36604 CHEBI:9177 denotes sodium deoxycholate
T18702 36656-36665 GO:0016020 denotes membranes
T18703 36716-36724 GO:0016020 denotes membrane
T18704 36749-36758 GO:0016020 denotes membranes
T18705 36849-36853 UBERON:0001977 denotes sera
T18706 36881-36890 PR:000029158 denotes protein A
T18707 37054-37062 GO:0016020 denotes membrane
T18708 37119-37131 GO:0009986 denotes Cell surface
T18709 37132-37145 MOP:0000093 denotes biotinylation
T18710 37196-37200 PR:000004182 denotes AQP2
T18711 37212-37223 GO:0009294 denotes transfected
T18712 37247-37257 GO:0010467 denotes expressing
T18713 37268-37272 PR:000004182 denotes AQP2
T18714 37300-37306 SO:0000440 denotes vector
T18715 37337-37349 GO:0009294 denotes transfection
T18716 37378-37387 CHEBI:42471 denotes forskolin
T18717 37489-37495 CHEBI:15956 denotes biotin
T18718 37583-37587 CHEBI:9754 denotes Tris
T18719 37631-37640 GO:0016020 denotes membranes
T18720 37703-37712 GO:0016020 denotes membranes
T18721 37927-37939 MOP:0000093 denotes biotinylated
T18722 37982-37990 GO:0042571 denotes antibody
T18723 37994-37998 PR:000004182 denotes AQP2
T19537 38001-38007 UBERON:0002113 denotes Kidney
T19538 38036-38041 NCBITaxon:10088 denotes mouse
T19539 38042-38049 UBERON:0002113 denotes kidneys
T19540 38087-38095 CHEBI:16842 denotes formalin
T19541 38106-38113 UBERON:0002113 denotes Kidneys
T19542 38184-38191 CHEBI:59132 denotes antigen
T19543 38214-38228 CHEBI:53258 denotes sodium citrate
T19544 38302-38306 PR:000004183 denotes AQP3
T19545 38320-38324 PR:000004182 denotes AQP2
T19546 38356-38362 NCBITaxon:9793 denotes donkey
T19547 38363-38368 UBERON:0001977 denotes serum
T19548 38381-38385 NCBITaxon:9925 denotes goat
T19549 38391-38395 PR:000004183 denotes AQP3
T19550 38396-38404 GO:0042571 denotes antibody
T19551 38497-38511 CHEBI:52661 denotes AlexaFluor 488
T19552 38512-38522 MOP:0000779 denotes conjugated
T19553 38523-38529 NCBITaxon:9793 denotes donkey
T19554 38535-38539 NCBITaxon:9925 denotes goat
T19555 38540-38548 GO:0042571 denotes antibody
T19556 38643-38650 NCBITaxon:9031 denotes chicken
T19557 38651-38656 UBERON:0001977 denotes serum
T19558 38675-38681 NCBITaxon:9986 denotes rabbit
T19559 38687-38691 PR:000004182 denotes AQP2
T19560 38692-38700 GO:0042571 denotes antibody
T19561 38784-38798 CHEBI:51248 denotes AlexaFluor 594
T19562 38799-38809 MOP:0000779 denotes conjugated
T19563 38810-38817 NCBITaxon:9031 denotes chicken
T19564 38823-38829 NCBITaxon:9986 denotes rabbit
T19565 38830-38838 GO:0042571 denotes antibody
T19566 38897-38901 CHEBI:51231 denotes DAPI
T20323 39042-39052 GO:0010467 denotes expressing
T20324 39053-39059 SO:0000440 denotes vector
T20325 39077-39081 PR:000004182 denotes AQP2
T20326 39086-39090 PR:000004182 denotes AQP2
T20327 39128-39138 GO:0010467 denotes expressing
T20328 39161-39166 GO:0040007 denotes grown
T20329 39170-39181 PR:000007581 denotes fibronectin
T20330 39272-39281 CHEBI:42471 denotes forskolin
T20331 39307-39315 CHEBI:17790 denotes methanol
T20332 39385-39397 CHEBI:9750 denotes Triton X-100
T20333 39437-39441 PR:000004182 denotes AQP2
T20334 39446-39455 GO:0043226 denotes organelle
T20335 39479-39481 GO:0005886 denotes PM
T20336 39489-39491 GO:0005783 denotes ER
T20337 39493-39497 PR:000004182 denotes AQP2
T20338 39517-39521 NCBITaxon:9925 denotes goat
T20339 39527-39531 PR:000004182 denotes AQP2
T20340 39600-39614 CHEBI:52661 denotes AlexaFluor 488
T20341 39615-39625 MOP:0000779 denotes conjugated
T20342 39626-39632 NCBITaxon:9793 denotes donkey
T20343 39638-39642 NCBITaxon:9925 denotes goat
T20344 39653-39661 GO:0042571 denotes antibody
T20345 39667-39669 GO:0005886 denotes PM
T20346 39674-39676 GO:0005783 denotes ER
T20347 39695-39700 NCBITaxon:10088 denotes mouse
T20348 39706-39709 CHEBI:29101 denotes Na+
T20349 39710-39712 CHEBI:29103 denotes K+
T20350 39772-39778 NCBITaxon:9986 denotes rabbit
T20351 39784-39792 PR:000005005 denotes calnexin
T20352 39855-39865 GO:0042571 denotes antibodies
T20353 39884-39894 GO:0042571 denotes antibodies
T20354 39896-39899 CHEBI:37987 denotes Cy3
T20355 39900-39910 MOP:0000779 denotes conjugated
T20356 39911-39915 NCBITaxon:9925 denotes goat
T20357 39921-39926 NCBITaxon:10088 denotes mouse
T20358 40003-40017 CHEBI:51248 denotes AlexaFluor 594
T20359 40018-40028 MOP:0000779 denotes conjugated
T20360 40029-40036 NCBITaxon:9031 denotes chicken
T20361 40042-40048 NCBITaxon:9986 denotes rabbit
T20362 40117-40121 CHEBI:51231 denotes DAPI
T20363 40198-40202 PR:000004182 denotes AQP2
T20364 40224-40232 GO:0042571 denotes antibody
T20365 40254-40260 UBERON:0002113 denotes kidney
T20366 40305-40309 PR:000004182 denotes AQP2
T8092 6718-6725 SO:0000704 denotes genetic
R240 T2212 T2211 _lexicallyChainedTo through,passage
R241 T2213 T2212 _lexicallyChainedTo membrane,through
R242 T2219 T2218 _lexicallyChainedTo in,water reabsorption
R243 T2220 T2219 _lexicallyChainedTo kidney,in
R244 T2226 T2225 _lexicallyChainedTo PM,regions of
R245 T2243 T2242 _lexicallyChainedTo cells,basolateral face of
R246 T2295 T2294 _lexicallyChainedTo cell,kidney
R906 T8204 T8203 _lexicallyChainedTo tubules,proximal
R907 T8114 T8113 _lexicallyChainedTo transversion,thymine to guanine
R908 T8114 T8115 _lexicallyChainedTo transversion,T to G
R909 T8244 T8243 _lexicallyChainedTo cells,region of
R911 T8284 T8283 _lexicallyChainedTo cells,surface of
R3376 T13875 T13874 _lexicallyChainedTo protein,transport of

craft-sa-dev

Id Subject Object Predicate Lexical cue
T593 0-8 NN denotes Diabetes
T594 9-18 NN denotes Insipidus
T595 0-57 sentence denotes Diabetes Insipidus in Mice with a Mutation in Aquaporin-2
T596 19-21 IN denotes in
T597 22-26 NNS denotes Mice
T598 27-31 IN denotes with
T599 32-33 DT denotes a
T600 34-42 NN denotes Mutation
T601 43-45 IN denotes in
T602 46-55 NN denotes Aquaporin
T603 55-56 HYPH denotes -
T604 56-57 CD denotes 2
T607 57-240 sentence denotes Aquaporin-2 Mutant Mice Abstract Congenital nephrogenic diabetes insipidus (NDI) is a disease characterized by failure of the kidney to concentrate urine in response to vasopressin.
T608 92-102 JJ denotes Congenital
T609 124-133 NN denotes insipidus
T610 103-114 JJ denotes nephrogenic
T611 115-123 NN denotes diabetes
T612 140-142 VBZ denotes is
T613 134-135 -LRB- denotes (
T614 135-138 NN denotes NDI
T615 138-139 -RRB- denotes )
T616 143-144 DT denotes a
T617 145-152 NN denotes disease
T618 153-166 VBN denotes characterized
T619 167-169 IN denotes by
T620 170-177 NN denotes failure
T621 178-180 IN denotes of
T622 181-184 DT denotes the
T623 185-191 NN denotes kidney
T624 192-194 TO denotes to
T625 195-206 VB denotes concentrate
T626 207-212 NN denotes urine
T627 213-215 IN denotes in
T628 216-224 NN denotes response
T629 225-227 IN denotes to
T630 228-239 NN denotes vasopressin
T631 239-240 . denotes .
T632 240-438 sentence denotes Human kindreds with nephrogenic diabetes insipidus have been found to harbor mutations in the vasopressin receptor 2 (Avpr2) gene or the vasopressin-sensitive water channel aquaporin-2 (Aqp2) gene.
T633 241-246 JJ denotes Human
T634 247-255 NNS denotes kindreds
T635 302-307 VBN denotes found
T636 256-260 IN denotes with
T637 261-272 JJ denotes nephrogenic
T638 282-291 NN denotes insipidus
T639 273-281 NN denotes diabetes
T640 292-296 VBP denotes have
T641 297-301 VBN denotes been
T642 308-310 TO denotes to
T643 311-317 VB denotes harbor
T644 318-327 NNS denotes mutations
T645 328-330 IN denotes in
T646 331-334 DT denotes the
T647 366-370 NN denotes gene
T648 335-346 NN denotes vasopressin
T649 347-355 NN denotes receptor
T650 356-357 CD denotes 2
T651 358-359 -LRB- denotes (
T652 359-364 NN denotes Avpr2
T653 364-365 -RRB- denotes )
T654 371-373 CC denotes or
T655 374-377 DT denotes the
T656 433-437 NN denotes gene
T657 378-389 NN denotes vasopressin
T658 390-399 JJ denotes sensitive
T659 389-390 HYPH denotes -
T660 400-405 NN denotes water
T661 406-413 NN denotes channel
T662 414-423 NN denotes aquaporin
T663 423-424 HYPH denotes -
T664 424-425 CD denotes 2
T665 426-427 -LRB- denotes (
T666 427-431 NN denotes Aqp2
T667 431-432 -RRB- denotes )
T668 437-438 . denotes .
T669 438-529 sentence denotes Development of a treatment is rendered difficult due to the lack of a viable animal model.
T670 439-450 NN denotes Development
T671 469-477 VBN denotes rendered
T672 451-453 IN denotes of
T673 454-455 DT denotes a
T674 456-465 NN denotes treatment
T675 466-468 VBZ denotes is
T676 478-487 JJ denotes difficult
T677 488-491 IN denotes due
T678 492-494 IN denotes to
T679 495-498 DT denotes the
T680 499-503 NN denotes lack
T681 504-506 IN denotes of
T682 507-508 DT denotes a
T683 523-528 NN denotes model
T684 509-515 JJ denotes viable
T685 516-522 NN denotes animal
T686 528-529 . denotes .
T687 529-717 sentence denotes Through forward genetic screening of ethylnitrosourea-mutagenized mice, we report the identification and characterization of a mouse model of NDI, with an F204V mutation in the Aqp2 gene.
T688 530-537 IN denotes Through
T689 605-611 VBP denotes report
T690 538-545 JJ denotes forward
T691 554-563 NN denotes screening
T692 546-553 JJ denotes genetic
T693 564-566 IN denotes of
T694 567-583 NN denotes ethylnitrosourea
T695 584-595 VBN denotes mutagenized
T696 583-584 HYPH denotes -
T697 596-600 NNS denotes mice
T698 600-602 , denotes ,
T699 602-604 PRP denotes we
T700 612-615 DT denotes the
T701 616-630 NN denotes identification
T702 631-634 CC denotes and
T703 635-651 NN denotes characterization
T704 652-654 IN denotes of
T705 655-656 DT denotes a
T706 663-668 NN denotes model
T707 657-662 NN denotes mouse
T708 669-671 IN denotes of
T709 672-675 NN denotes NDI
T710 675-677 , denotes ,
T711 677-681 IN denotes with
T712 682-684 DT denotes an
T713 691-699 NN denotes mutation
T714 685-690 NN denotes F204V
T715 700-702 IN denotes in
T716 703-706 DT denotes the
T717 712-716 NN denotes gene
T718 707-711 NN denotes Aqp2
T719 716-717 . denotes .
T720 717-847 sentence denotes Unlike previously attempted murine models of NDI, our mice survive to adulthood and more exactly recapitulate the human disorder.
T721 718-724 IN denotes Unlike
T722 777-784 VBP denotes survive
T723 725-735 RB denotes previously
T724 736-745 VBN denotes attempted
T725 753-759 NNS denotes models
T726 746-752 JJ denotes murine
T727 760-762 IN denotes of
T728 763-766 NN denotes NDI
T729 766-768 , denotes ,
T730 768-771 PRP$ denotes our
T731 772-776 NNS denotes mice
T732 785-787 IN denotes to
T733 788-797 NN denotes adulthood
T734 798-801 CC denotes and
T735 802-806 RBR denotes more
T736 807-814 RB denotes exactly
T737 815-827 VBP denotes recapitulate
T738 828-831 DT denotes the
T739 838-846 NN denotes disorder
T740 832-837 JJ denotes human
T741 846-847 . denotes .
T742 847-990 sentence denotes Previous in vitro experiments using renal cell lines suggest recessive Aqp2 mutations result in improper trafficking of the mutant water pore.
T743 848-856 JJ denotes Previous
T744 866-877 NNS denotes experiments
T745 857-859 FW denotes in
T746 860-865 FW denotes vitro
T747 901-908 VBP denotes suggest
T748 878-883 VBG denotes using
T749 884-889 JJ denotes renal
T750 895-900 NNS denotes lines
T751 890-894 NN denotes cell
T752 909-918 JJ denotes recessive
T753 924-933 NNS denotes mutations
T754 919-923 NN denotes Aqp2
T755 934-940 VBP denotes result
T756 941-943 IN denotes in
T757 944-952 JJ denotes improper
T758 953-964 NN denotes trafficking
T759 965-967 IN denotes of
T760 968-971 DT denotes the
T761 985-989 NN denotes pore
T762 972-978 JJ denotes mutant
T763 979-984 NN denotes water
T764 989-990 . denotes .
T765 990-1164 sentence denotes Using these animals, we have directly proven this hypothesis of improper AQP2 translocation as the molecular defect in nephrogenic diabetes insipidus in the intact organism.
T766 991-996 VBG denotes Using
T767 1029-1035 VBN denotes proven
T768 997-1002 DT denotes these
T769 1003-1010 NNS denotes animals
T770 1010-1012 , denotes ,
T771 1012-1014 PRP denotes we
T772 1015-1019 VBP denotes have
T773 1020-1028 RB denotes directly
T774 1036-1040 DT denotes this
T775 1041-1051 NN denotes hypothesis
T776 1052-1054 IN denotes of
T777 1055-1063 JJ denotes improper
T778 1069-1082 NN denotes translocation
T779 1064-1068 NN denotes AQP2
T780 1083-1085 IN denotes as
T781 1086-1089 DT denotes the
T782 1100-1106 NN denotes defect
T783 1090-1099 JJ denotes molecular
T784 1107-1109 IN denotes in
T785 1110-1121 JJ denotes nephrogenic
T786 1131-1140 NN denotes insipidus
T787 1122-1130 NN denotes diabetes
T788 1141-1143 IN denotes in
T789 1144-1147 DT denotes the
T790 1155-1163 NN denotes organism
T791 1148-1154 JJ denotes intact
T792 1163-1164 . denotes .
T793 1164-1362 sentence denotes Additionally, using a renal cell line we show that the mutated protein, AQP2-F204V, is retained in the endoplasmic reticulum and that this abnormal localization can be rescued by wild-type protein.
T794 1165-1177 RB denotes Additionally
T795 1206-1210 VBP denotes show
T796 1177-1179 , denotes ,
T797 1179-1184 VBG denotes using
T798 1185-1186 DT denotes a
T799 1198-1202 NN denotes line
T800 1187-1192 JJ denotes renal
T801 1193-1197 NN denotes cell
T802 1203-1205 PRP denotes we
T803 1211-1215 IN denotes that
T804 1252-1260 VBN denotes retained
T805 1216-1219 DT denotes the
T806 1228-1235 NN denotes protein
T807 1220-1227 VBN denotes mutated
T808 1235-1237 , denotes ,
T809 1237-1241 NN denotes AQP2
T810 1242-1247 NN denotes F204V
T811 1241-1242 HYPH denotes -
T812 1247-1249 , denotes ,
T813 1249-1251 VBZ denotes is
T814 1261-1263 IN denotes in
T815 1264-1267 DT denotes the
T816 1280-1289 NN denotes reticulum
T817 1268-1279 JJ denotes endoplasmic
T818 1290-1293 CC denotes and
T819 1294-1298 IN denotes that
T820 1333-1340 VBN denotes rescued
T821 1299-1303 DT denotes this
T822 1313-1325 NN denotes localization
T823 1304-1312 JJ denotes abnormal
T824 1326-1329 MD denotes can
T825 1330-1332 VB denotes be
T826 1341-1343 IN denotes by
T827 1344-1348 JJ denotes wild
T828 1349-1353 NN denotes type
T829 1348-1349 HYPH denotes -
T830 1354-1361 NN denotes protein
T831 1361-1362 . denotes .
T832 1362-1490 sentence denotes This novel mouse model allows for further mechanistic studies as well as testing of pharmacological and gene therapies for NDI.
T833 1363-1367 DT denotes This
T834 1380-1385 NN denotes model
T835 1368-1373 JJ denotes novel
T836 1374-1379 NN denotes mouse
T837 1386-1392 VBZ denotes allows
T838 1393-1396 IN denotes for
T839 1397-1404 JJ denotes further
T840 1417-1424 NNS denotes studies
T841 1405-1416 JJ denotes mechanistic
T842 1425-1427 RB denotes as
T843 1433-1435 IN denotes as
T844 1428-1432 RB denotes well
T845 1436-1443 NN denotes testing
T846 1444-1446 IN denotes of
T847 1447-1462 JJ denotes pharmacological
T848 1472-1481 NNS denotes therapies
T849 1463-1466 CC denotes and
T850 1467-1471 NN denotes gene
T851 1482-1485 IN denotes for
T852 1486-1489 NN denotes NDI
T853 1489-1490 . denotes .
T2464 3091-3102 JJ denotes Nephrogenic
T2465 3112-3121 NN denotes insipidus
T2466 3103-3111 NN denotes diabetes
T2467 3128-3130 VBZ denotes is
T2468 3122-3123 -LRB- denotes (
T2469 3123-3126 NN denotes NDI
T2470 3126-3127 -RRB- denotes )
T2471 3131-3132 DT denotes a
T2472 3133-3140 NN denotes disease
T2473 3141-3154 VBN denotes characterized
T2474 3155-3157 IN denotes by
T2475 3158-3167 JJ denotes excessive
T2476 3168-3177 NN denotes urination
T2477 3178-3181 CC denotes and
T2478 3182-3188 RB denotes thirst
T2479 3188-3190 , denotes ,
T2480 3190-3197 IN denotes despite
T2481 3198-3204 JJ denotes normal
T2482 3205-3215 NN denotes production
T2483 3216-3218 IN denotes of
T2484 3219-3222 DT denotes the
T2485 3236-3243 NN denotes hormone
T2486 3223-3235 JJ denotes antidiuretic
T2487 3244-3252 NN denotes arginine
T2488 3253-3264 NN denotes vasopressin
T2489 3265-3266 -LRB- denotes (
T2490 3266-3269 NN denotes AVP
T2491 3269-3270 -RRB- denotes )
T2492 3271-3272 -LRB- denotes [
T2493 3272-3273 CD denotes 1
T2494 3273-3274 -RRB- denotes ]
T2495 3274-3275 . denotes .
T2496 3275-3418 sentence denotes The inherited forms are either X-linked as a consequence of mutation of the Avpr2 gene [2], or autosomal due to mutation of the Aqp2 gene [3].
T2497 3276-3279 DT denotes The
T2498 3290-3295 NNS denotes forms
T2499 3280-3289 VBN denotes inherited
T2500 3296-3299 VBP denotes are
T2501 3300-3306 RB denotes either
T2502 3309-3315 VBN denotes linked
T2503 3307-3308 NN denotes X
T2504 3308-3309 HYPH denotes -
T2505 3316-3318 IN denotes as
T2506 3319-3320 DT denotes a
T2507 3321-3332 NN denotes consequence
T2508 3333-3335 IN denotes of
T2509 3336-3344 NN denotes mutation
T2510 3345-3347 IN denotes of
T2511 3348-3351 DT denotes the
T2512 3358-3362 NN denotes gene
T2513 3352-3357 NN denotes Avpr2
T2514 3363-3364 -LRB- denotes [
T2515 3364-3365 CD denotes 2
T2516 3365-3366 -RRB- denotes ]
T2517 3366-3368 , denotes ,
T2518 3368-3370 CC denotes or
T2519 3371-3380 JJ denotes autosomal
T2520 3381-3384 JJ denotes due
T2521 3385-3387 IN denotes to
T2522 3388-3396 NN denotes mutation
T2523 3397-3399 IN denotes of
T2524 3400-3403 DT denotes the
T2525 3409-3413 NN denotes gene
T2526 3404-3408 NN denotes Aqp2
T2527 3414-3415 -LRB- denotes [
T2528 3415-3416 CD denotes 3
T2529 3416-3417 -RRB- denotes ]
T2530 3417-3418 . denotes .
T2531 3418-3582 sentence denotes Aquaporin-2 (AQP2) is a pore-forming protein belonging to a family of water channels [4], and it is expressed in collecting-duct principal cells in the kidney [5].
T2532 3419-3428 NN denotes Aquaporin
T2533 3438-3440 VBZ denotes is
T2534 3428-3429 HYPH denotes -
T2535 3429-3430 CD denotes 2
T2536 3431-3432 -LRB- denotes (
T2537 3432-3436 NN denotes AQP2
T2538 3436-3437 -RRB- denotes )
T2539 3441-3442 DT denotes a
T2540 3456-3463 NN denotes protein
T2541 3443-3447 NN denotes pore
T2542 3448-3455 VBG denotes forming
T2543 3447-3448 HYPH denotes -
T2544 3464-3473 VBG denotes belonging
T2545 3474-3476 IN denotes to
T2546 3477-3478 DT denotes a
T2547 3479-3485 NN denotes family
T2548 3486-3488 IN denotes of
T2549 3489-3494 NN denotes water
T2550 3495-3503 NNS denotes channels
T2551 3504-3505 -LRB- denotes [
T2552 3505-3506 CD denotes 4
T2553 3506-3507 -RRB- denotes ]
T2554 3507-3509 , denotes ,
T2555 3509-3512 CC denotes and
T2556 3513-3515 PRP denotes it
T2557 3519-3528 VBN denotes expressed
T2558 3516-3518 VBZ denotes is
T2559 3529-3531 IN denotes in
T2560 3532-3542 VBG denotes collecting
T2561 3543-3547 NN denotes duct
T2562 3542-3543 HYPH denotes -
T2563 3558-3563 NNS denotes cells
T2564 3548-3557 JJ denotes principal
T2565 3564-3566 IN denotes in
T2566 3567-3570 DT denotes the
T2567 3571-3577 NN denotes kidney
T2568 3578-3579 -LRB- denotes [
T2569 3579-3580 CD denotes 5
T2570 3580-3581 -RRB- denotes ]
T2571 3581-3582 . denotes .
T2572 3582-3791 sentence denotes Generally these proteins permit the passage of water through the plasma membrane (PM) of cells, several of which carry out this role specifically in the process of water reabsorption from urine in the kidney.
T2573 3583-3592 RB denotes Generally
T2574 3608-3614 VBP denotes permit
T2575 3593-3598 DT denotes these
T2576 3599-3607 NN denotes proteins
T2577 3615-3618 DT denotes the
T2578 3619-3626 NN denotes passage
T2579 3627-3629 IN denotes of
T2580 3630-3635 NN denotes water
T2581 3636-3643 IN denotes through
T2582 3644-3647 DT denotes the
T2583 3655-3663 NN denotes membrane
T2584 3648-3654 NN denotes plasma
T2585 3664-3665 -LRB- denotes (
T2586 3665-3667 NN denotes PM
T2587 3667-3668 -RRB- denotes )
T2588 3669-3671 IN denotes of
T2589 3672-3677 NNS denotes cells
T2590 3677-3679 , denotes ,
T2591 3679-3686 JJ denotes several
T2592 3696-3701 VBP denotes carry
T2593 3687-3689 IN denotes of
T2594 3690-3695 WDT denotes which
T2595 3702-3705 RP denotes out
T2596 3706-3710 DT denotes this
T2597 3711-3715 NN denotes role
T2598 3716-3728 RB denotes specifically
T2599 3729-3731 IN denotes in
T2600 3732-3735 DT denotes the
T2601 3736-3743 NN denotes process
T2602 3744-3746 IN denotes of
T2603 3747-3752 NN denotes water
T2604 3753-3765 NN denotes reabsorption
T2605 3766-3770 IN denotes from
T2606 3771-3776 NN denotes urine
T2607 3777-3779 IN denotes in
T2608 3780-3783 DT denotes the
T2609 3784-3790 NN denotes kidney
T2610 3790-3791 . denotes .
T2611 3791-3933 sentence denotes It has been established that aquaporins, although functional as a monomer, tetramerize before their insertion into the plasma membrane [4,6].
T2612 3792-3794 PRP denotes It
T2613 3804-3815 VBN denotes established
T2614 3795-3798 VBZ denotes has
T2615 3799-3803 VBN denotes been
T2616 3816-3820 IN denotes that
T2617 3867-3878 VBP denotes tetramerize
T2618 3821-3831 NNS denotes aquaporins
T2619 3831-3833 , denotes ,
T2620 3833-3841 IN denotes although
T2621 3842-3852 JJ denotes functional
T2622 3853-3855 IN denotes as
T2623 3856-3857 DT denotes a
T2624 3858-3865 NN denotes monomer
T2625 3865-3867 , denotes ,
T2626 3879-3885 IN denotes before
T2627 3886-3891 PRP$ denotes their
T2628 3892-3901 NN denotes insertion
T2629 3902-3906 IN denotes into
T2630 3907-3910 DT denotes the
T2631 3918-3926 NN denotes membrane
T2632 3911-3917 NN denotes plasma
T2633 3927-3928 -LRB- denotes [
T2634 3930-3931 CD denotes 6
T2635 3928-3929 CD denotes 4
T2636 3929-3930 , denotes ,
T2637 3931-3932 -RRB- denotes ]
T2638 3932-3933 . denotes .
T2639 3933-4197 sentence denotes Furthermore these proteins can also be differentially targeted to distinct regions of the PM; for example, AQP2 is routed to the apical membrane of cells surrounding the collecting duct, whereas other aquaporins (AQP3 or 4) are inserted into the basolateral face.
T2640 3934-3945 RB denotes Furthermore
T2641 3988-3996 VBN denotes targeted
T2642 3946-3951 DT denotes these
T2643 3952-3960 NN denotes proteins
T2644 3961-3964 MD denotes can
T2645 3965-3969 RB denotes also
T2646 3970-3972 VB denotes be
T2647 3973-3987 RB denotes differentially
T2648 4049-4055 VBN denotes routed
T2649 3997-3999 IN denotes to
T2650 4000-4008 JJ denotes distinct
T2651 4009-4016 NNS denotes regions
T2652 4017-4019 IN denotes of
T2653 4020-4023 DT denotes the
T2654 4024-4026 NN denotes PM
T2655 4026-4027 : denotes ;
T2656 4028-4031 IN denotes for
T2657 4032-4039 NN denotes example
T2658 4039-4041 , denotes ,
T2659 4041-4045 NN denotes AQP2
T2660 4046-4048 VBZ denotes is
T2661 4056-4058 IN denotes to
T2662 4059-4062 DT denotes the
T2663 4070-4078 NN denotes membrane
T2664 4063-4069 JJ denotes apical
T2665 4079-4081 IN denotes of
T2666 4082-4087 NNS denotes cells
T2667 4088-4099 VBG denotes surrounding
T2668 4100-4103 DT denotes the
T2669 4115-4119 NN denotes duct
T2670 4104-4114 VBG denotes collecting
T2671 4119-4121 , denotes ,
T2672 4121-4128 IN denotes whereas
T2673 4162-4170 VBN denotes inserted
T2674 4129-4134 JJ denotes other
T2675 4135-4145 NNS denotes aquaporins
T2676 4146-4147 -LRB- denotes (
T2677 4147-4151 NN denotes AQP3
T2678 4152-4154 CC denotes or
T2679 4155-4156 CD denotes 4
T2680 4156-4157 -RRB- denotes )
T2681 4158-4161 VBP denotes are
T2682 4171-4175 IN denotes into
T2683 4176-4179 DT denotes the
T2684 4192-4196 NN denotes face
T2685 4180-4191 JJ denotes basolateral
T2686 4196-4197 . denotes .
T2687 4197-4292 sentence denotes Unlike all other family members, AQP2 is not constitutively inserted into the plasma membrane.
T2688 4198-4204 IN denotes Unlike
T2689 4258-4266 VBN denotes inserted
T2690 4205-4208 DT denotes all
T2691 4222-4229 NNS denotes members
T2692 4209-4214 JJ denotes other
T2693 4215-4221 NN denotes family
T2694 4229-4231 , denotes ,
T2695 4231-4235 NN denotes AQP2
T2696 4236-4238 VBZ denotes is
T2697 4239-4242 RB denotes not
T2698 4243-4257 RB denotes constitutively
T2699 4267-4271 IN denotes into
T2700 4272-4275 DT denotes the
T2701 4283-4291 NN denotes membrane
T2702 4276-4282 NN denotes plasma
T2703 4291-4292 . denotes .
T2704 4292-4504 sentence denotes Under basal conditions, the protein resides in subapical intracellular vesicles; however, under conditions requiring water retention AQP2 translocates to the apical membrane, permitting water reabsorption [7,8].
T2705 4293-4298 IN denotes Under
T2706 4329-4336 VBZ denotes resides
T2707 4299-4304 JJ denotes basal
T2708 4305-4315 NNS denotes conditions
T2709 4315-4317 , denotes ,
T2710 4317-4320 DT denotes the
T2711 4321-4328 NN denotes protein
T2712 4431-4443 VBZ denotes translocates
T2713 4337-4339 IN denotes in
T2714 4340-4349 JJ denotes subapical
T2715 4364-4372 NNS denotes vesicles
T2716 4350-4363 JJ denotes intracellular
T2717 4372-4373 : denotes ;
T2718 4374-4381 RB denotes however
T2719 4381-4383 , denotes ,
T2720 4383-4388 IN denotes under
T2721 4389-4399 NNS denotes conditions
T2722 4400-4409 VBG denotes requiring
T2723 4410-4415 NN denotes water
T2724 4416-4425 NN denotes retention
T2725 4426-4430 NN denotes AQP2
T2726 4444-4446 IN denotes to
T2727 4447-4450 DT denotes the
T2728 4458-4466 NN denotes membrane
T2729 4451-4457 JJ denotes apical
T2730 4466-4468 , denotes ,
T2731 4468-4478 VBG denotes permitting
T2732 4479-4484 NN denotes water
T2733 4485-4497 NN denotes reabsorption
T2734 4498-4499 -LRB- denotes [
T2735 4501-4502 CD denotes 8
T2736 4499-4500 CD denotes 7
T2737 4500-4501 , denotes ,
T2738 4502-4503 -RRB- denotes ]
T2739 4503-4504 . denotes .
T2740 4504-4803 sentence denotes For this process to occur, AVP binds its receptor, AVPR2, on the basolateral face of the collecting duct cells, leading to a rise in intracellular cAMP, ultimately resulting in phosphorylation of AQP2 at serine 256 by cAMP-dependent protein kinase [9] and its redistribution to the plasma membrane.
T2741 4505-4508 IN denotes For
T2742 4525-4530 VB denotes occur
T2743 4509-4513 DT denotes this
T2744 4514-4521 NN denotes process
T2745 4522-4524 TO denotes to
T2746 4536-4541 VBZ denotes binds
T2747 4530-4532 , denotes ,
T2748 4532-4535 NN denotes AVP
T2749 4542-4545 PRP$ denotes its
T2750 4546-4554 NN denotes receptor
T2751 4554-4556 , denotes ,
T2752 4556-4561 NN denotes AVPR2
T2753 4561-4563 , denotes ,
T2754 4563-4565 IN denotes on
T2755 4566-4569 DT denotes the
T2756 4582-4586 NN denotes face
T2757 4570-4581 JJ denotes basolateral
T2758 4587-4589 IN denotes of
T2759 4590-4593 DT denotes the
T2760 4610-4615 NNS denotes cells
T2761 4594-4604 VBG denotes collecting
T2762 4605-4609 NN denotes duct
T2763 4615-4617 , denotes ,
T2764 4617-4624 VBG denotes leading
T2765 4625-4627 IN denotes to
T2766 4628-4629 DT denotes a
T2767 4630-4634 NN denotes rise
T2768 4635-4637 IN denotes in
T2769 4638-4651 JJ denotes intracellular
T2770 4652-4656 NN denotes cAMP
T2771 4656-4658 , denotes ,
T2772 4658-4668 RB denotes ultimately
T2773 4669-4678 VBG denotes resulting
T2774 4679-4681 IN denotes in
T2775 4682-4697 NN denotes phosphorylation
T2776 4698-4700 IN denotes of
T2777 4701-4705 NN denotes AQP2
T2778 4706-4708 IN denotes at
T2779 4709-4715 NN denotes serine
T2780 4716-4719 CD denotes 256
T2781 4720-4722 IN denotes by
T2782 4723-4727 NN denotes cAMP
T2783 4728-4737 JJ denotes dependent
T2784 4727-4728 HYPH denotes -
T2785 4746-4752 NN denotes kinase
T2786 4738-4745 NN denotes protein
T2787 4753-4754 -LRB- denotes [
T2788 4754-4755 CD denotes 9
T2789 4755-4756 -RRB- denotes ]
T2790 4757-4760 CC denotes and
T2791 4761-4764 PRP$ denotes its
T2792 4765-4779 NN denotes redistribution
T2793 4780-4782 IN denotes to
T2794 4783-4786 DT denotes the
T2795 4794-4802 NN denotes membrane
T2796 4787-4793 NN denotes plasma
T2797 4802-4803 . denotes .
T2798 4803-4953 sentence denotes The importance of AQP2 redistribution has been highlighted by functional characterization of Aqp2 mutations resulting in severe NDI in humans [3,10].
T2799 4804-4807 DT denotes The
T2800 4808-4818 NN denotes importance
T2801 4851-4862 VBN denotes highlighted
T2802 4819-4821 IN denotes of
T2803 4822-4826 NN denotes AQP2
T2804 4827-4841 NN denotes redistribution
T2805 4842-4845 VBZ denotes has
T2806 4846-4850 VBN denotes been
T2807 4863-4865 IN denotes by
T2808 4866-4876 JJ denotes functional
T2809 4877-4893 NN denotes characterization
T2810 4894-4896 IN denotes of
T2811 4897-4901 NN denotes Aqp2
T2812 4902-4911 NNS denotes mutations
T2813 4912-4921 VBG denotes resulting
T2814 4922-4924 IN denotes in
T2815 4925-4931 JJ denotes severe
T2816 4932-4935 NN denotes NDI
T2817 4936-4938 IN denotes in
T2818 4939-4945 NNS denotes humans
T2819 4946-4947 -LRB- denotes [
T2820 4949-4951 CD denotes 10
T2821 4947-4948 CD denotes 3
T2822 4948-4949 , denotes ,
T2823 4951-4952 -RRB- denotes ]
T2824 4952-4953 . denotes .
T2825 4953-5136 sentence denotes Recessive Aqp2 mutations are generally thought to produce an abnormally localized and, in most instances, misfolded water pore that responds abnormally to an increase in cAMP [6,11].
T2826 4954-4963 JJ denotes Recessive
T2827 4969-4978 NNS denotes mutations
T2828 4964-4968 NN denotes Aqp2
T2829 4993-5000 VBN denotes thought
T2830 4979-4982 VBP denotes are
T2831 4983-4992 RB denotes generally
T2832 5001-5003 TO denotes to
T2833 5004-5011 VB denotes produce
T2834 5012-5014 DT denotes an
T2835 5076-5080 NN denotes pore
T2836 5015-5025 RB denotes abnormally
T2837 5026-5035 VBN denotes localized
T2838 5036-5039 CC denotes and
T2839 5039-5041 , denotes ,
T2840 5060-5069 VBN denotes misfolded
T2841 5041-5043 IN denotes in
T2842 5044-5048 JJS denotes most
T2843 5049-5058 NNS denotes instances
T2844 5058-5060 , denotes ,
T2845 5070-5075 NN denotes water
T2846 5081-5085 WDT denotes that
T2847 5086-5094 VBZ denotes responds
T2848 5095-5105 RB denotes abnormally
T2849 5106-5108 IN denotes to
T2850 5109-5111 DT denotes an
T2851 5112-5120 NN denotes increase
T2852 5121-5123 IN denotes in
T2853 5124-5128 NN denotes cAMP
T2854 5129-5130 -LRB- denotes [
T2855 5132-5134 CD denotes 11
T2856 5130-5131 CD denotes 6
T2857 5131-5132 , denotes ,
T2858 5134-5135 -RRB- denotes ]
T2859 5135-5136 . denotes .
T2860 5136-5288 sentence denotes Furthermore, dominant mutations have been described and found to misroute both the mutant and the wild-type protein to the basolateral membrane [6,12].
T2861 5137-5148 RB denotes Furthermore
T2862 5179-5188 VBN denotes described
T2863 5148-5150 , denotes ,
T2864 5150-5158 JJ denotes dominant
T2865 5159-5168 NNS denotes mutations
T2866 5169-5173 VBP denotes have
T2867 5174-5178 VBN denotes been
T2868 5189-5192 CC denotes and
T2869 5193-5198 VBN denotes found
T2870 5199-5201 TO denotes to
T2871 5202-5210 VB denotes misroute
T2872 5211-5215 CC denotes both
T2873 5220-5226 NN denotes mutant
T2874 5216-5219 DT denotes the
T2875 5227-5230 CC denotes and
T2876 5231-5234 DT denotes the
T2877 5240-5244 NN denotes type
T2878 5235-5239 JJ denotes wild
T2879 5239-5240 HYPH denotes -
T2880 5245-5252 NN denotes protein
T2881 5253-5255 IN denotes to
T2882 5256-5259 DT denotes the
T2883 5272-5280 NN denotes membrane
T2884 5260-5271 JJ denotes basolateral
T2885 5281-5282 -LRB- denotes [
T2886 5284-5286 CD denotes 12
T2887 5282-5283 CD denotes 6
T2888 5283-5284 , denotes ,
T2889 5286-5287 -RRB- denotes ]
T2890 5287-5288 . denotes .
T2891 5288-5360 sentence denotes Several mouse models of diabetes insipidus have been generated [13–17].
T2892 5289-5296 JJ denotes Several
T2893 5303-5309 NNS denotes models
T2894 5297-5302 NN denotes mouse
T2895 5342-5351 VBN denotes generated
T2896 5310-5312 IN denotes of
T2897 5313-5321 NN denotes diabetes
T2898 5322-5331 NN denotes insipidus
T2899 5332-5336 VBP denotes have
T2900 5337-5341 VBN denotes been
T2901 5352-5353 -LRB- denotes [
T2902 5353-5355 CD denotes 13
T2903 5355-5356 SYM denotes –
T2904 5356-5358 CD denotes 17
T2905 5358-5359 -RRB- denotes ]
T2906 5359-5360 . denotes .
T2907 5360-5468 sentence denotes In an attempt to recapitulate human NDI, mice have been generated with mutations in Aqp2 and Avpr2 [15,18].
T2908 5361-5363 IN denotes In
T2909 5417-5426 VBN denotes generated
T2910 5364-5366 DT denotes an
T2911 5367-5374 NN denotes attempt
T2912 5375-5377 TO denotes to
T2913 5378-5390 VB denotes recapitulate
T2914 5391-5396 JJ denotes human
T2915 5397-5400 NN denotes NDI
T2916 5400-5402 , denotes ,
T2917 5402-5406 NNS denotes mice
T2918 5407-5411 VBP denotes have
T2919 5412-5416 VBN denotes been
T2920 5427-5431 IN denotes with
T2921 5432-5441 NNS denotes mutations
T2922 5442-5444 IN denotes in
T2923 5445-5449 NN denotes Aqp2
T2924 5450-5453 CC denotes and
T2925 5454-5459 NN denotes Avpr2
T2926 5460-5461 -LRB- denotes [
T2927 5464-5466 CD denotes 18
T2928 5461-5463 CD denotes 15
T2929 5463-5464 , denotes ,
T2930 5466-5467 -RRB- denotes ]
T2931 5467-5468 . denotes .
T2932 5468-5553 sentence denotes Yang and colleagues created a mouse with a T126M knock-in mutation in the Aqp2 gene.
T2933 5469-5473 NNP denotes Yang
T2934 5489-5496 VBD denotes created
T2935 5474-5477 CC denotes and
T2936 5478-5488 NNS denotes colleagues
T2937 5497-5498 DT denotes a
T2938 5499-5504 NN denotes mouse
T2939 5505-5509 IN denotes with
T2940 5510-5511 DT denotes a
T2941 5527-5535 NN denotes mutation
T2942 5512-5517 NN denotes T126M
T2943 5518-5523 VB denotes knock
T2944 5523-5524 HYPH denotes -
T2945 5524-5526 RP denotes in
T2946 5536-5538 IN denotes in
T2947 5539-5542 DT denotes the
T2948 5548-5552 NN denotes gene
T2949 5543-5547 NN denotes Aqp2
T2950 5552-5553 . denotes .
T2951 5553-5619 sentence denotes Unexpectedly, homozygous mutant mice died within 6 d after birth.
T2952 5554-5566 RB denotes Unexpectedly
T2953 5591-5595 VBD denotes died
T2954 5566-5568 , denotes ,
T2955 5568-5578 JJ denotes homozygous
T2956 5586-5590 NNS denotes mice
T2957 5579-5585 NN denotes mutant
T2958 5596-5602 IN denotes within
T2959 5603-5604 CD denotes 6
T2960 5605-5606 NNS denotes d
T2961 5607-5612 IN denotes after
T2962 5613-5618 NN denotes birth
T2963 5618-5619 . denotes .
T2964 5619-5704 sentence denotes Interestingly, AVPR2-deficient male pups also die within the first week after birth.
T2965 5620-5633 RB denotes Interestingly
T2966 5666-5669 VBP denotes die
T2967 5633-5635 , denotes ,
T2968 5635-5640 NN denotes AVPR2
T2969 5641-5650 JJ denotes deficient
T2970 5640-5641 HYPH denotes -
T2971 5656-5660 NNS denotes pups
T2972 5651-5655 JJ denotes male
T2973 5661-5665 RB denotes also
T2974 5670-5676 IN denotes within
T2975 5677-5680 DT denotes the
T2976 5687-5691 NN denotes week
T2977 5681-5686 JJ denotes first
T2978 5692-5697 IN denotes after
T2979 5698-5703 NN denotes birth
T2980 5703-5704 . denotes .
T2981 5704-5858 sentence denotes Together these models suggest that the mouse may be a highly sensitive organism with regard to water homeostasis, and is unable to survive with polyuria.
T2982 5705-5713 RB denotes Together
T2983 5727-5734 VBP denotes suggest
T2984 5714-5719 DT denotes these
T2985 5720-5726 NNS denotes models
T2986 5735-5739 IN denotes that
T2987 5754-5756 VB denotes be
T2988 5740-5743 DT denotes the
T2989 5744-5749 NN denotes mouse
T2990 5750-5753 MD denotes may
T2991 5757-5758 DT denotes a
T2992 5776-5784 NN denotes organism
T2993 5759-5765 RB denotes highly
T2994 5766-5775 JJ denotes sensitive
T2995 5785-5789 IN denotes with
T2996 5790-5796 NN denotes regard
T2997 5797-5799 IN denotes to
T2998 5800-5805 NN denotes water
T2999 5806-5817 NN denotes homeostasis
T3000 5817-5819 , denotes ,
T3001 5819-5822 CC denotes and
T3002 5823-5825 VBZ denotes is
T3003 5826-5832 JJ denotes unable
T3004 5833-5835 TO denotes to
T3005 5836-5843 VB denotes survive
T3006 5844-5848 IN denotes with
T3007 5849-5857 NN denotes polyuria
T3008 5857-5858 . denotes .
T3009 5858-5933 sentence denotes In a forward genetic screen, a mouse with an Aqp2 mutation was identified.
T3010 5859-5861 IN denotes In
T3011 5922-5932 VBN denotes identified
T3012 5862-5863 DT denotes a
T3013 5880-5886 NN denotes screen
T3014 5864-5871 RB denotes forward
T3015 5872-5879 JJ denotes genetic
T3016 5886-5888 , denotes ,
T3017 5888-5889 DT denotes a
T3018 5890-5895 NN denotes mouse
T3019 5896-5900 IN denotes with
T3020 5901-5903 DT denotes an
T3021 5909-5917 NN denotes mutation
T3022 5904-5908 NN denotes Aqp2
T3023 5918-5921 VBD denotes was
T3024 5932-5933 . denotes .
T3025 5933-6026 sentence denotes The purpose of this study was to characterize this murine model of recessive nephrogenic DI.
T3026 5934-5937 DT denotes The
T3027 5938-5945 NN denotes purpose
T3028 5960-5963 VBD denotes was
T3029 5946-5948 IN denotes of
T3030 5949-5953 DT denotes this
T3031 5954-5959 NN denotes study
T3032 5964-5966 TO denotes to
T3033 5967-5979 VB denotes characterize
T3034 5980-5984 DT denotes this
T3035 5992-5997 NN denotes model
T3036 5985-5991 JJ denotes murine
T3037 5998-6000 IN denotes of
T3038 6001-6010 JJ denotes recessive
T3039 6023-6025 NN denotes DI
T3040 6011-6022 JJ denotes nephrogenic
T3041 6025-6026 . denotes .
T3042 6026-6081 sentence denotes We now report a novel F204V mutation in the Aqp2 gene.
T3043 6027-6029 PRP denotes We
T3044 6034-6040 VBP denotes report
T3045 6030-6033 RB denotes now
T3046 6041-6042 DT denotes a
T3047 6055-6063 NN denotes mutation
T3048 6043-6048 JJ denotes novel
T3049 6049-6054 NN denotes F204V
T3050 6064-6066 IN denotes in
T3051 6067-6070 DT denotes the
T3052 6076-6080 NN denotes gene
T3053 6071-6075 NN denotes Aqp2
T3054 6080-6081 . denotes .
T3055 6081-6189 sentence denotes This allele of Aqp2 was found to cause the first mouse model of NDI to survive past the first week of life.
T3056 6082-6086 DT denotes This
T3057 6087-6093 NN denotes allele
T3058 6106-6111 VBN denotes found
T3059 6094-6096 IN denotes of
T3060 6097-6101 NN denotes Aqp2
T3061 6102-6105 VBD denotes was
T3062 6112-6114 TO denotes to
T3063 6115-6120 VB denotes cause
T3064 6121-6124 DT denotes the
T3065 6137-6142 NN denotes model
T3066 6125-6130 JJ denotes first
T3067 6131-6136 NN denotes mouse
T3068 6153-6160 VB denotes survive
T3069 6143-6145 IN denotes of
T3070 6146-6149 NN denotes NDI
T3071 6150-6152 TO denotes to
T3072 6161-6165 IN denotes past
T3073 6166-6169 DT denotes the
T3074 6176-6180 NN denotes week
T3075 6170-6175 JJ denotes first
T3076 6181-6183 IN denotes of
T3077 6184-6188 NN denotes life
T3078 6188-6189 . denotes .
T3079 6189-6390 sentence denotes Molecular analyses concluded that mutant AQP2 adopts a different subcellular localization in renal collecting-duct cells, and was resistant to translocation induced by desmopressin, an agonist of AVP.
T3080 6190-6199 JJ denotes Molecular
T3081 6200-6208 NNS denotes analyses
T3082 6209-6218 VBD denotes concluded
T3083 6219-6223 IN denotes that
T3084 6236-6242 VBZ denotes adopts
T3085 6224-6230 NN denotes mutant
T3086 6231-6235 NN denotes AQP2
T3087 6243-6244 DT denotes a
T3088 6267-6279 NN denotes localization
T3089 6245-6254 JJ denotes different
T3090 6255-6266 JJ denotes subcellular
T3091 6280-6282 IN denotes in
T3092 6283-6288 JJ denotes renal
T3093 6305-6310 NNS denotes cells
T3094 6289-6299 VBG denotes collecting
T3095 6300-6304 NN denotes duct
T3096 6299-6300 HYPH denotes -
T3097 6310-6312 , denotes ,
T3098 6312-6315 CC denotes and
T3099 6316-6319 VBD denotes was
T3100 6320-6329 JJ denotes resistant
T3101 6330-6332 IN denotes to
T3102 6333-6346 NN denotes translocation
T3103 6347-6354 VBN denotes induced
T3104 6355-6357 IN denotes by
T3105 6358-6370 NN denotes desmopressin
T3106 6370-6372 , denotes ,
T3107 6372-6374 DT denotes an
T3108 6375-6382 NN denotes agonist
T3109 6383-6385 IN denotes of
T3110 6386-6389 NN denotes AVP
T3111 6389-6390 . denotes .
T3112 6390-6572 sentence denotes In vitro studies using the Madin-Darby canine kidney (MDCK) cell line demonstrated an endoplasmic reticulum pattern for the mutant protein, and apparent resistance to translocation.
T3113 6391-6393 FW denotes In
T3114 6394-6399 FW denotes vitro
T3115 6400-6407 NNS denotes studies
T3116 6461-6473 VBD denotes demonstrated
T3117 6408-6413 VBG denotes using
T3118 6414-6417 DT denotes the
T3119 6456-6460 NN denotes line
T3120 6418-6423 NN denotes Madin
T3121 6424-6429 NN denotes Darby
T3122 6423-6424 HYPH denotes -
T3123 6437-6443 NN denotes kidney
T3124 6430-6436 JJ denotes canine
T3125 6444-6445 -LRB- denotes (
T3126 6445-6449 NN denotes MDCK
T3127 6449-6450 -RRB- denotes )
T3128 6451-6455 NN denotes cell
T3129 6474-6476 DT denotes an
T3130 6499-6506 NN denotes pattern
T3131 6477-6488 JJ denotes endoplasmic
T3132 6489-6498 NN denotes reticulum
T3133 6507-6510 IN denotes for
T3134 6511-6514 DT denotes the
T3135 6522-6529 NN denotes protein
T3136 6515-6521 NN denotes mutant
T3137 6529-6531 , denotes ,
T3138 6531-6534 CC denotes and
T3139 6535-6543 JJ denotes apparent
T3140 6544-6554 NN denotes resistance
T3141 6555-6557 IN denotes to
T3142 6558-6571 NN denotes translocation
T3143 6571-6572 . denotes .
T3144 6572-6695 sentence denotes These data conclusively prove that autosomal recessive NDI is a consequence of improper AQP2 routing in the intact mammal.
T3145 6573-6578 DT denotes These
T3146 6579-6583 NNS denotes data
T3147 6597-6602 VBP denotes prove
T3148 6584-6596 RB denotes conclusively
T3149 6603-6607 IN denotes that
T3150 6632-6634 VBZ denotes is
T3151 6608-6617 JJ denotes autosomal
T3152 6628-6631 NN denotes NDI
T3153 6618-6627 JJ denotes recessive
T3154 6635-6636 DT denotes a
T3155 6637-6648 NN denotes consequence
T3156 6649-6651 IN denotes of
T3157 6652-6660 JJ denotes improper
T3158 6666-6673 NN denotes routing
T3159 6661-6665 NN denotes AQP2
T3160 6674-6676 IN denotes in
T3161 6677-6680 DT denotes the
T3162 6688-6694 NN denotes mammal
T3163 6681-6687 JJ denotes intact
T3164 6694-6695 . denotes .
T11284 24296-24297 -LRB- denotes (
T11285 24314-24318 JJ denotes left
T11286 24297-24303 NN denotes Figure
T11287 24304-24306 CD denotes 5C
T11288 24306-24308 , denotes ,
T11289 24308-24313 JJ denotes lower
T11290 24318-24319 -RRB- denotes )
T11291 24319-24320 . denotes .
T11292 24320-24476 sentence denotes When expressed in wild-type AQP2 cells, however, AQP2-F204V-GFP localized to the apical surface to varying degrees (Figure 5C, lower right images [i–iii]).
T8951 6705-6707 IN denotes In
T8952 6888-6893 VBD denotes found
T8953 6708-6709 DT denotes a
T8954 6726-6732 NN denotes screen
T8955 6710-6717 JJ denotes forward
T8956 6718-6725 JJ denotes genetic
T8957 6733-6737 WDT denotes that
T8958 6738-6742 VBD denotes used
T8959 6743-6759 NN denotes ethylnitrosourea
T8960 6760-6761 -LRB- denotes (
T8961 6761-6764 NN denotes ENU
T8962 6764-6765 -RRB- denotes )
T8963 6766-6768 TO denotes to
T8964 6769-6775 VB denotes induce
T8965 6776-6785 NNS denotes mutations
T8966 6786-6788 IN denotes in
T8967 6789-6790 DT denotes a
T8968 6799-6805 NN denotes animal
T8969 6791-6798 NN denotes founder
T8970 6806-6811 WP$ denotes whose
T8971 6812-6821 NN denotes offspring
T8972 6832-6840 VBN denotes screened
T8973 6822-6826 VBD denotes were
T8974 6827-6831 RB denotes then
T8975 6841-6844 IN denotes for
T8976 6845-6853 JJ denotes abnormal
T8977 6865-6875 NN denotes metabolism
T8978 6854-6859 JJ denotes whole
T8979 6860-6864 NN denotes body
T8980 6876-6877 -LRB- denotes [
T8981 6880-6882 CD denotes 20
T8982 6877-6879 CD denotes 19
T8983 6879-6880 , denotes ,
T8984 6882-6883 -RRB- denotes ]
T8985 6883-6885 , denotes ,
T8986 6885-6887 PRP denotes we
T8987 6894-6895 DT denotes a
T8988 6896-6902 NN denotes family
T8989 6903-6905 IN denotes of
T8990 6906-6910 NNS denotes mice
T8991 6911-6915 WDT denotes that
T8992 6916-6924 VBD denotes urinated
T8993 6925-6928 CC denotes and
T8994 6929-6934 VBD denotes drank
T8995 6935-6946 RB denotes excessively
T8996 6946-6947 . denotes .
T8997 6947-7076 sentence denotes Serum and urine analysis showed that plasma glucose levels were normal and there was no glucose in the urine (unpublished data).
T8998 6948-6953 NN denotes Serum
T8999 6964-6972 NN denotes analysis
T9000 6954-6957 CC denotes and
T9001 6958-6963 NN denotes urine
T9002 6973-6979 VBD denotes showed
T9003 6980-6984 IN denotes that
T9004 7007-7011 VBD denotes were
T9005 6985-6991 NN denotes plasma
T9006 7000-7006 NNS denotes levels
T9007 6992-6999 NN denotes glucose
T9008 7012-7018 JJ denotes normal
T9009 7019-7022 CC denotes and
T9010 7023-7028 EX denotes there
T9011 7029-7032 VBD denotes was
T9012 7033-7035 DT denotes no
T9013 7036-7043 NN denotes glucose
T9014 7044-7046 IN denotes in
T9015 7047-7050 DT denotes the
T9016 7051-7056 NN denotes urine
T9017 7057-7058 -LRB- denotes (
T9018 7070-7074 NNS denotes data
T9019 7058-7069 JJ denotes unpublished
T9020 7074-7075 -RRB- denotes )
T9021 7075-7076 . denotes .
T9022 7076-7126 sentence denotes Hence, this was an example of diabetes insipidus.
T9023 7077-7082 RB denotes Hence
T9024 7089-7092 VBD denotes was
T9025 7082-7084 , denotes ,
T9026 7084-7088 DT denotes this
T9027 7093-7095 DT denotes an
T9028 7096-7103 NN denotes example
T9029 7104-7106 IN denotes of
T9030 7107-7115 NN denotes diabetes
T9031 7116-7125 NN denotes insipidus
T9032 7125-7126 . denotes .
T9033 7126-7238 sentence denotes The disorder in these mice segregated in a monogenic, autosomal recessive manner, making Aqp2 a candidate gene.
T9034 7127-7130 DT denotes The
T9035 7131-7139 NN denotes disorder
T9036 7154-7164 VBD denotes segregated
T9037 7140-7142 IN denotes in
T9038 7143-7148 DT denotes these
T9039 7149-7153 NNS denotes mice
T9040 7165-7167 IN denotes in
T9041 7168-7169 DT denotes a
T9042 7201-7207 NN denotes manner
T9043 7170-7179 JJ denotes monogenic
T9044 7179-7181 , denotes ,
T9045 7181-7190 JJ denotes autosomal
T9046 7191-7200 JJ denotes recessive
T9047 7207-7209 , denotes ,
T9048 7209-7215 VBG denotes making
T9049 7216-7220 NN denotes Aqp2
T9050 7233-7237 NN denotes gene
T9051 7221-7222 DT denotes a
T9052 7223-7232 NN denotes candidate
T9053 7237-7238 . denotes .
T9054 7238-7467 sentence denotes Sequencing of Aqp2 coding region of affected mice identified a thymine to guanine (T to G) transversion (Figure 1A), which is predicted to lead to a valine for phenylalanine substitution at amino acid 204 of the protein (F204V).
T9055 7239-7249 NN denotes Sequencing
T9056 7289-7299 VBD denotes identified
T9057 7250-7252 IN denotes of
T9058 7253-7257 NN denotes Aqp2
T9059 7265-7271 NN denotes region
T9060 7258-7264 NN denotes coding
T9061 7272-7274 IN denotes of
T9062 7275-7283 VBN denotes affected
T9063 7284-7288 NNS denotes mice
T9064 7300-7301 DT denotes a
T9065 7330-7342 NN denotes transversion
T9066 7302-7309 NN denotes thymine
T9067 7310-7312 IN denotes to
T9068 7313-7320 NN denotes guanine
T9069 7321-7322 -LRB- denotes (
T9070 7322-7323 NN denotes T
T9071 7324-7326 IN denotes to
T9072 7327-7328 NN denotes G
T9073 7328-7329 -RRB- denotes )
T9074 7343-7344 -LRB- denotes (
T9075 7344-7350 NN denotes Figure
T9076 7351-7353 CD denotes 1A
T9077 7353-7354 -RRB- denotes )
T9078 7354-7356 , denotes ,
T9079 7356-7361 WDT denotes which
T9080 7365-7374 VBN denotes predicted
T9081 7362-7364 VBZ denotes is
T9082 7375-7377 TO denotes to
T9083 7378-7382 VB denotes lead
T9084 7383-7385 IN denotes to
T9085 7386-7387 DT denotes a
T9086 7413-7425 NN denotes substitution
T9087 7388-7394 NN denotes valine
T9088 7395-7398 IN denotes for
T9089 7399-7412 NN denotes phenylalanine
T9090 7426-7428 IN denotes at
T9091 7429-7434 NN denotes amino
T9092 7435-7439 NN denotes acid
T9093 7440-7443 CD denotes 204
T9094 7444-7446 IN denotes of
T9095 7447-7450 DT denotes the
T9096 7451-7458 NN denotes protein
T9097 7459-7460 -LRB- denotes (
T9098 7460-7465 NN denotes F204V
T9099 7465-7466 -RRB- denotes )
T9100 7466-7467 . denotes .
T9101 7467-8657 sentence denotes Figure 1 Analysis of Aqp2 Sequence and Phenotype in Mutant Mice (A) Chromatographic traces of Aqp2 F204V mutation. The box shows the mutated codon, TTC (Phe) to GTC (Val) at position 204. WT, wild type; Mut, mutant. (B) Amino acid conservation of mouse AQP2 (residues 194–214). The boxed residue indicates phenylalanine at position 204. hAQP2, human AQP2; mAQP1, mouse AQP1; mAQP2, mouse AQP2; rAQP2, rat AQP2; xAQP2, Xenopus AQP2. (C) Urine production (ml) and water consumption (ml) of 58 F2 mice over a 24-h period (both sexes, aged 10–22 wk). Mutant mice (black squares) exhibit overt polyuria and polydipsia compared to littermate wild-type (white triangles) and heterozygous (grey circles) mice. (D) Urine osmolality and concentrating ability in Aqp2 mutant and their littermates (10–22 wk, both sexes), before (white bars) and after (black bars) dDAVP treatment. Wild type (WT; n = 12); heterozygote (Het; n = 20); mutant (Mut; n = 9). Data represent averages ± standard error of the mean, **p < 0.01; ***p < 0.001. AQP2 is a six-transmembrane water channel, and F204 lies near the extracellular face of the sixth membrane spanning domain, a region rich in hydrophobic amino acids.
T9102 8492-8496 NN denotes AQP2
T9103 8497-8499 VBZ denotes is
T9104 8500-8501 DT denotes a
T9105 8526-8533 NN denotes channel
T9106 8502-8505 CD denotes six
T9107 8506-8519 NN denotes transmembrane
T9108 8505-8506 HYPH denotes -
T9109 8520-8525 NN denotes water
T9110 8533-8535 , denotes ,
T9111 8535-8538 CC denotes and
T9112 8539-8543 NN denotes F204
T9113 8544-8548 VBZ denotes lies
T9114 8549-8553 IN denotes near
T9115 8554-8557 DT denotes the
T9116 8572-8576 NN denotes face
T9117 8558-8571 JJ denotes extracellular
T9118 8577-8579 IN denotes of
T9119 8580-8583 DT denotes the
T9120 8608-8614 NN denotes domain
T9121 8584-8589 JJ denotes sixth
T9122 8590-8598 NN denotes membrane
T9123 8599-8607 VBG denotes spanning
T9124 8614-8616 , denotes ,
T9125 8616-8617 DT denotes a
T9126 8618-8624 NN denotes region
T9127 8625-8629 JJ denotes rich
T9128 8630-8632 IN denotes in
T9129 8633-8644 JJ denotes hydrophobic
T9130 8651-8656 NNS denotes acids
T9131 8645-8650 NN denotes amino
T9132 8656-8657 . denotes .
T9133 8657-8742 sentence denotes This and the other membrane-spanning domains are conserved among vertebrate species.
T9134 8658-8662 DT denotes This
T9135 8707-8716 VBN denotes conserved
T9136 8663-8666 CC denotes and
T9137 8667-8670 DT denotes the
T9138 8671-8676 JJ denotes other
T9139 8695-8702 NNS denotes domains
T9140 8677-8685 NN denotes membrane
T9141 8686-8694 VBG denotes spanning
T9142 8685-8686 HYPH denotes -
T9143 8703-8706 VBP denotes are
T9144 8717-8722 IN denotes among
T9145 8723-8733 JJ denotes vertebrate
T9146 8734-8741 NNS denotes species
T9147 8741-8742 . denotes .
T9148 8742-8907 sentence denotes The phenylalanine at position 204 is particularly well conserved (Figure 1B), not only among vertebrate AQP2 proteins, but also among others members of this family.
T9149 8743-8746 DT denotes The
T9150 8747-8760 NN denotes phenylalanine
T9151 8777-8779 VBZ denotes is
T9152 8761-8763 IN denotes at
T9153 8764-8772 NN denotes position
T9154 8773-8776 CD denotes 204
T9155 8780-8792 RB denotes particularly
T9156 8798-8807 VBN denotes conserved
T9157 8793-8797 RB denotes well
T9158 8808-8809 -LRB- denotes (
T9159 8809-8815 NN denotes Figure
T9160 8816-8818 CD denotes 1B
T9161 8818-8819 -RRB- denotes )
T9162 8819-8821 , denotes ,
T9163 8821-8824 RB denotes not
T9164 8830-8835 IN denotes among
T9165 8825-8829 RB denotes only
T9166 8836-8846 JJ denotes vertebrate
T9167 8852-8860 NN denotes proteins
T9168 8847-8851 NN denotes AQP2
T9169 8860-8862 , denotes ,
T9170 8862-8865 CC denotes but
T9171 8866-8870 RB denotes also
T9172 8871-8876 IN denotes among
T9173 8877-8883 NNS denotes others
T9174 8884-8891 NNS denotes members
T9175 8892-8894 IN denotes of
T9176 8895-8899 DT denotes this
T9177 8900-8906 NN denotes family
T9178 8906-8907 . denotes .
T9179 8907-9114 sentence denotes Aqp2F204V/F204V mice have dramatically increased urine production, in some cases producing an amount of urine in 24 h that exceeds their body weight, compared to their heterozygous or wild-type littermates.
T9180 8908-8917 NN denotes Aqp2F204V
T9181 8918-8923 NN denotes F204V
T9182 8917-8918 HYPH denotes /
T9183 8924-8928 NNS denotes mice
T9184 8929-8933 VBP denotes have
T9185 8934-8946 RB denotes dramatically
T9186 8947-8956 VBN denotes increased
T9187 8963-8973 NN denotes production
T9188 8957-8962 NN denotes urine
T9189 8973-8975 , denotes ,
T9190 8975-8977 IN denotes in
T9191 8989-8998 VBG denotes producing
T9192 8978-8982 DT denotes some
T9193 8983-8988 NNS denotes cases
T9194 8999-9001 DT denotes an
T9195 9002-9008 NN denotes amount
T9196 9009-9011 IN denotes of
T9197 9012-9017 NN denotes urine
T9198 9018-9020 IN denotes in
T9199 9021-9023 CD denotes 24
T9200 9024-9025 NN denotes h
T9201 9026-9030 WDT denotes that
T9202 9031-9038 VBZ denotes exceeds
T9203 9039-9044 PRP$ denotes their
T9204 9050-9056 NN denotes weight
T9205 9045-9049 NN denotes body
T9206 9056-9058 , denotes ,
T9207 9058-9066 VBN denotes compared
T9208 9067-9069 IN denotes to
T9209 9070-9075 PRP$ denotes their
T9210 9102-9113 NNS denotes littermates
T9211 9076-9088 JJ denotes heterozygous
T9212 9089-9091 CC denotes or
T9213 9092-9096 JJ denotes wild
T9214 9097-9101 NN denotes type
T9215 9096-9097 HYPH denotes -
T9216 9113-9114 . denotes .
T9217 9114-9218 sentence denotes Such loss of water would rapidly lead to dehydration were it not compensated by increased water intake.
T9218 9115-9119 JJ denotes Such
T9219 9120-9124 NN denotes loss
T9220 9148-9152 VB denotes lead
T9221 9125-9127 IN denotes of
T9222 9128-9133 NN denotes water
T9223 9134-9139 MD denotes would
T9224 9140-9147 RB denotes rapidly
T9225 9153-9155 IN denotes to
T9226 9156-9167 NN denotes dehydration
T9227 9168-9172 VBD denotes were
T9228 9180-9191 VBN denotes compensated
T9229 9173-9175 PRP denotes it
T9230 9176-9179 RB denotes not
T9231 9192-9194 IN denotes by
T9232 9195-9204 VBN denotes increased
T9233 9211-9217 NN denotes intake
T9234 9205-9210 NN denotes water
T9235 9217-9218 . denotes .
T9236 9218-9353 sentence denotes Indeed, mutant mice also dramatically increase their water intake (Figure 1C) compared to their heterozygous or wild-type littermates.
T9237 9219-9225 RB denotes Indeed
T9238 9257-9265 VB denotes increase
T9239 9225-9227 , denotes ,
T9240 9227-9233 NN denotes mutant
T9241 9234-9238 NNS denotes mice
T9242 9239-9243 RB denotes also
T9243 9244-9256 RB denotes dramatically
T9244 9266-9271 PRP$ denotes their
T9245 9278-9284 NN denotes intake
T9246 9272-9277 NN denotes water
T9247 9285-9286 -LRB- denotes (
T9248 9286-9292 NN denotes Figure
T9249 9293-9295 CD denotes 1C
T9250 9295-9296 -RRB- denotes )
T9251 9297-9305 VBN denotes compared
T9252 9306-9308 IN denotes to
T9253 9309-9314 PRP$ denotes their
T9254 9341-9352 NNS denotes littermates
T9255 9315-9327 JJ denotes heterozygous
T9256 9328-9330 CC denotes or
T9257 9331-9335 JJ denotes wild
T9258 9336-9340 NN denotes type
T9259 9335-9336 HYPH denotes -
T9260 9352-9353 . denotes .
T9261 9353-9504 sentence denotes This phenotype—increased urinary output and water intake—showed complete concordance with homozygosity of the F204V mutation in the 58 animals tested.
T9262 9354-9358 DT denotes This
T9263 9359-9368 NN denotes phenotype
T9264 9411-9417 VBD denotes showed
T9265 9368-9369 , denotes —
T9266 9369-9378 VBN denotes increased
T9267 9387-9393 NN denotes output
T9268 9379-9386 JJ denotes urinary
T9269 9394-9397 CC denotes and
T9270 9398-9403 NN denotes water
T9271 9404-9410 NN denotes intake
T9272 9410-9411 , denotes —
T9273 9418-9426 JJ denotes complete
T9274 9427-9438 NN denotes concordance
T9275 9439-9443 IN denotes with
T9276 9444-9456 NN denotes homozygosity
T9277 9457-9459 IN denotes of
T9278 9460-9463 DT denotes the
T9279 9470-9478 NN denotes mutation
T9280 9464-9469 NN denotes F204V
T9281 9479-9481 IN denotes in
T9282 9482-9485 DT denotes the
T9283 9489-9496 NNS denotes animals
T9284 9486-9488 CD denotes 58
T9285 9497-9503 VBN denotes tested
T9286 9503-9504 . denotes .
T9287 9504-9594 sentence denotes Diabetes insipidus can be defined as an inability to concentrate urine where appropriate.
T9288 9505-9513 NN denotes Diabetes
T9289 9514-9523 NN denotes insipidus
T9290 9531-9538 VBN denotes defined
T9291 9524-9527 MD denotes can
T9292 9528-9530 VB denotes be
T9293 9539-9541 IN denotes as
T9294 9542-9544 DT denotes an
T9295 9545-9554 NN denotes inability
T9296 9555-9557 TO denotes to
T9297 9558-9569 VB denotes concentrate
T9298 9570-9575 NN denotes urine
T9299 9576-9581 WRB denotes where
T9300 9582-9593 JJ denotes appropriate
T9301 9593-9594 . denotes .
T9302 9594-9705 sentence denotes Compared to wild-type or heterozygous littermates, Aqp2F204V/F204V mice produce very dilute urine (Figure 1D).
T9303 9595-9603 VBN denotes Compared
T9304 9667-9674 VBP denotes produce
T9305 9604-9606 IN denotes to
T9306 9607-9611 JJ denotes wild
T9307 9612-9616 NN denotes type
T9308 9611-9612 HYPH denotes -
T9309 9633-9644 NNS denotes littermates
T9310 9617-9619 CC denotes or
T9311 9620-9632 JJ denotes heterozygous
T9312 9644-9646 , denotes ,
T9313 9646-9655 NN denotes Aqp2F204V
T9314 9656-9661 NN denotes F204V
T9315 9655-9656 HYPH denotes /
T9316 9662-9666 NNS denotes mice
T9317 9675-9679 RB denotes very
T9318 9680-9686 JJ denotes dilute
T9319 9687-9692 NN denotes urine
T9320 9693-9694 -LRB- denotes (
T9321 9694-9700 NN denotes Figure
T9322 9701-9703 CD denotes 1D
T9323 9703-9704 -RRB- denotes )
T9324 9704-9705 . denotes .
T9325 9705-9825 sentence denotes Basal urine concentration in mutant mice is about 161 mOsm, compared to about 1,293 mOsm in wild-type mice (p < 0.001).
T9326 9706-9711 JJ denotes Basal
T9327 9718-9731 NN denotes concentration
T9328 9712-9717 NN denotes urine
T9329 9747-9749 VBZ denotes is
T9330 9732-9734 IN denotes in
T9331 9735-9741 NN denotes mutant
T9332 9742-9746 NNS denotes mice
T9333 9750-9755 RB denotes about
T9334 9756-9759 CD denotes 161
T9335 9760-9764 NN denotes mOsm
T9336 9764-9766 , denotes ,
T9337 9766-9774 VBN denotes compared
T9338 9775-9777 IN denotes to
T9339 9778-9783 IN denotes about
T9340 9784-9789 CD denotes 1,293
T9341 9790-9794 NN denotes mOsm
T9342 9795-9797 IN denotes in
T9343 9798-9802 JJ denotes wild
T9344 9803-9807 NN denotes type
T9345 9802-9803 HYPH denotes -
T9346 9808-9812 NNS denotes mice
T9347 9813-9814 -LRB- denotes (
T9348 9818-9823 CD denotes 0.001
T9349 9814-9815 NN denotes p
T9350 9816-9817 SYM denotes <
T9351 9823-9824 -RRB- denotes )
T9352 9824-9825 . denotes .
T9353 9825-9968 sentence denotes Normally, urine concentration is under the control of the hypothalamus, which, in response to hypovolemia or hypernatremia [21], secretes AVP.
T9354 9826-9834 RB denotes Normally
T9355 9856-9858 VBZ denotes is
T9356 9834-9836 , denotes ,
T9357 9836-9841 NN denotes urine
T9358 9842-9855 NN denotes concentration
T9359 9859-9864 IN denotes under
T9360 9865-9868 DT denotes the
T9361 9869-9876 NN denotes control
T9362 9877-9879 IN denotes of
T9363 9880-9883 DT denotes the
T9364 9884-9896 NN denotes hypothalamus
T9365 9896-9898 , denotes ,
T9366 9898-9903 WDT denotes which
T9367 9955-9963 VBZ denotes secretes
T9368 9903-9905 , denotes ,
T9369 9905-9907 IN denotes in
T9370 9908-9916 NN denotes response
T9371 9917-9919 IN denotes to
T9372 9920-9931 NN denotes hypovolemia
T9373 9932-9934 CC denotes or
T9374 9935-9948 NN denotes hypernatremia
T9375 9949-9950 -LRB- denotes [
T9376 9950-9952 CD denotes 21
T9377 9952-9953 -RRB- denotes ]
T9378 9953-9955 , denotes ,
T9379 9964-9967 NN denotes AVP
T9380 9967-9968 . denotes .
T9381 9968-10094 sentence denotes The synthetic AVP analog, 1-deamino-8-D-arginine vasopressin (dDAVP; also called desmopressin), is a potent agonist of AVPR2.
T9382 9969-9972 DT denotes The
T9383 9987-9993 NN denotes analog
T9384 9973-9982 JJ denotes synthetic
T9385 9983-9986 NN denotes AVP
T9386 10065-10067 VBZ denotes is
T9387 9993-9995 , denotes ,
T9388 9995-9996 CD denotes 1
T9389 9997-10004 NN denotes deamino
T9390 9996-9997 HYPH denotes -
T9391 10009-10017 NN denotes arginine
T9392 10004-10005 HYPH denotes -
T9393 10005-10006 CD denotes 8
T9394 10006-10007 HYPH denotes -
T9395 10007-10008 NN denotes D
T9396 10008-10009 HYPH denotes -
T9397 10018-10029 NN denotes vasopressin
T9398 10030-10031 -LRB- denotes (
T9399 10031-10036 NN denotes dDAVP
T9400 10036-10037 : denotes ;
T9401 10038-10042 RB denotes also
T9402 10043-10049 VBN denotes called
T9403 10050-10062 NN denotes desmopressin
T9404 10062-10063 -RRB- denotes )
T9405 10063-10065 , denotes ,
T9406 10068-10069 DT denotes a
T9407 10077-10084 NN denotes agonist
T9408 10070-10076 JJ denotes potent
T9409 10085-10087 IN denotes of
T9410 10088-10093 NN denotes AVPR2
T9411 10093-10094 . denotes .
T9412 10094-10238 sentence denotes When administered to wild-type mice, dDAVP leads to a dramatic increase in urine concentration, from 1,293 to 5,885 mOsm (4.6-fold; Figure 1D).
T9413 10095-10099 WRB denotes When
T9414 10100-10112 VBN denotes administered
T9415 10138-10143 VBZ denotes leads
T9416 10113-10115 IN denotes to
T9417 10116-10120 JJ denotes wild
T9418 10121-10125 NN denotes type
T9419 10120-10121 HYPH denotes -
T9420 10126-10130 NNS denotes mice
T9421 10130-10132 , denotes ,
T9422 10132-10137 NN denotes dDAVP
T9423 10144-10146 IN denotes to
T9424 10147-10148 DT denotes a
T9425 10158-10166 NN denotes increase
T9426 10149-10157 JJ denotes dramatic
T9427 10167-10169 IN denotes in
T9428 10170-10175 NN denotes urine
T9429 10176-10189 NN denotes concentration
T9430 10189-10191 , denotes ,
T9431 10191-10195 IN denotes from
T9432 10196-10201 CD denotes 1,293
T9433 10202-10204 IN denotes to
T9434 10205-10210 CD denotes 5,885
T9435 10211-10215 NN denotes mOsm
T9436 10216-10217 -LRB- denotes (
T9437 10227-10233 NN denotes Figure
T9438 10217-10225 RB denotes 4.6-fold
T9439 10225-10226 : denotes ;
T9440 10234-10236 CD denotes 1D
T9441 10236-10237 -RRB- denotes )
T9442 10237-10238 . denotes .
T9443 10238-10510 sentence denotes With similar treatment, mutant mice concentrate their urine to a lesser but still significant extent, from 161 to 470 mOsm (2.9-fold), indicating that these animals are not only unable to concentrate their urine properly but are also defective in their response to dDAVP.
T9444 10239-10243 IN denotes With
T9445 10275-10286 VBP denotes concentrate
T9446 10244-10251 JJ denotes similar
T9447 10252-10261 NN denotes treatment
T9448 10261-10263 , denotes ,
T9449 10263-10269 NN denotes mutant
T9450 10270-10274 NNS denotes mice
T9451 10287-10292 PRP$ denotes their
T9452 10293-10298 NN denotes urine
T9453 10299-10301 IN denotes to
T9454 10302-10303 DT denotes a
T9455 10333-10339 NN denotes extent
T9456 10304-10310 JJR denotes lesser
T9457 10311-10314 CC denotes but
T9458 10315-10320 RB denotes still
T9459 10321-10332 JJ denotes significant
T9460 10339-10341 , denotes ,
T9461 10341-10345 IN denotes from
T9462 10346-10349 CD denotes 161
T9463 10350-10352 IN denotes to
T9464 10353-10356 CD denotes 470
T9465 10357-10361 NN denotes mOsm
T9466 10362-10363 -LRB- denotes (
T9467 10363-10371 RB denotes 2.9-fold
T9468 10371-10372 -RRB- denotes )
T9469 10372-10374 , denotes ,
T9470 10374-10384 VBG denotes indicating
T9471 10385-10389 IN denotes that
T9472 10404-10407 VBP denotes are
T9473 10390-10395 DT denotes these
T9474 10396-10403 NNS denotes animals
T9475 10408-10411 RB denotes not
T9476 10412-10416 RB denotes only
T9477 10417-10423 JJ denotes unable
T9478 10424-10426 TO denotes to
T9479 10427-10438 VB denotes concentrate
T9480 10439-10444 PRP$ denotes their
T9481 10445-10450 NN denotes urine
T9482 10451-10459 RB denotes properly
T9483 10460-10463 CC denotes but
T9484 10464-10467 VBP denotes are
T9485 10468-10472 RB denotes also
T9486 10473-10482 JJ denotes defective
T9487 10483-10485 IN denotes in
T9488 10486-10491 PRP$ denotes their
T9489 10492-10500 NN denotes response
T9490 10501-10503 IN denotes to
T9491 10504-10509 NN denotes dDAVP
T9492 10509-10510 . denotes .
T9493 10510-10709 sentence denotes The smaller response to dDAVP indicates some residual activity of the mutant AQP2 channel, which must be sufficient to allow survival of the individual, in contrast to the T126M knock-in mouse [18].
T9494 10511-10514 DT denotes The
T9495 10523-10531 NN denotes response
T9496 10515-10522 JJR denotes smaller
T9497 10541-10550 VBZ denotes indicates
T9498 10532-10534 IN denotes to
T9499 10535-10540 NN denotes dDAVP
T9500 10551-10555 DT denotes some
T9501 10565-10573 NN denotes activity
T9502 10556-10564 JJ denotes residual
T9503 10574-10576 IN denotes of
T9504 10577-10580 DT denotes the
T9505 10593-10600 NN denotes channel
T9506 10581-10587 NN denotes mutant
T9507 10588-10592 NN denotes AQP2
T9508 10600-10602 , denotes ,
T9509 10602-10607 WDT denotes which
T9510 10613-10615 VB denotes be
T9511 10608-10612 MD denotes must
T9512 10616-10626 JJ denotes sufficient
T9513 10627-10629 TO denotes to
T9514 10630-10635 VB denotes allow
T9515 10636-10644 NN denotes survival
T9516 10645-10647 IN denotes of
T9517 10648-10651 DT denotes the
T9518 10652-10662 NN denotes individual
T9519 10662-10664 , denotes ,
T9520 10664-10666 IN denotes in
T9521 10667-10675 NN denotes contrast
T9522 10676-10678 IN denotes to
T9523 10679-10682 DT denotes the
T9524 10698-10703 NN denotes mouse
T9525 10683-10688 NN denotes T126M
T9526 10689-10694 VB denotes knock
T9527 10694-10695 HYPH denotes -
T9528 10695-10697 RP denotes in
T9529 10704-10705 -LRB- denotes [
T9530 10705-10707 CD denotes 18
T9531 10707-10708 -RRB- denotes ]
T9532 10708-10709 . denotes .
T9533 10709-10967 sentence denotes Multiple heterozygous matings yielded 101 animals, which appeared at a ratio of 26:49:26, near the expected Mendelian wild type, heterozygote, and mutant frequencies, respectively, indicating that there is no reduced viability associated with this mutation.
T9534 10710-10718 JJ denotes Multiple
T9535 10732-10739 NNS denotes matings
T9536 10719-10731 JJ denotes heterozygous
T9537 10740-10747 VBD denotes yielded
T9538 10748-10751 CD denotes 101
T9539 10752-10759 NNS denotes animals
T9540 10759-10761 , denotes ,
T9541 10761-10766 WDT denotes which
T9542 10767-10775 VBD denotes appeared
T9543 10776-10778 IN denotes at
T9544 10779-10780 DT denotes a
T9545 10781-10786 NN denotes ratio
T9546 10787-10789 IN denotes of
T9547 10790-10792 CD denotes 26
T9548 10792-10793 SYM denotes :
T9549 10793-10795 CD denotes 49
T9550 10795-10796 SYM denotes :
T9551 10796-10798 CD denotes 26
T9552 10798-10800 , denotes ,
T9553 10800-10804 IN denotes near
T9554 10805-10808 DT denotes the
T9555 10864-10875 NNS denotes frequencies
T9556 10809-10817 VBN denotes expected
T9557 10818-10827 JJ denotes Mendelian
T9558 10833-10837 NN denotes type
T9559 10828-10832 JJ denotes wild
T9560 10837-10839 , denotes ,
T9561 10839-10851 NN denotes heterozygote
T9562 10851-10853 , denotes ,
T9563 10853-10856 CC denotes and
T9564 10857-10863 NN denotes mutant
T9565 10875-10877 , denotes ,
T9566 10877-10889 RB denotes respectively
T9567 10889-10891 , denotes ,
T9568 10891-10901 VBG denotes indicating
T9569 10902-10906 IN denotes that
T9570 10913-10915 VBZ denotes is
T9571 10907-10912 EX denotes there
T9572 10916-10918 DT denotes no
T9573 10927-10936 NN denotes viability
T9574 10919-10926 VBN denotes reduced
T9575 10937-10947 VBN denotes associated
T9576 10948-10952 IN denotes with
T9577 10953-10957 DT denotes this
T9578 10958-10966 NN denotes mutation
T9579 10966-10967 . denotes .
T9580 10967-11150 sentence denotes Other than the increased urine production and water intake, there was no overt phenotype in mutant mice, save distended kidneys, which appeared variably in adult animals (Figure 2A).
T9581 10968-10973 JJ denotes Other
T9582 11034-11037 VBD denotes was
T9583 10974-10978 IN denotes than
T9584 10979-10982 DT denotes the
T9585 10999-11009 NN denotes production
T9586 10983-10992 VBN denotes increased
T9587 10993-10998 NN denotes urine
T9588 11010-11013 CC denotes and
T9589 11014-11019 NN denotes water
T9590 11020-11026 NN denotes intake
T9591 11026-11028 , denotes ,
T9592 11028-11033 EX denotes there
T9593 11038-11040 DT denotes no
T9594 11047-11056 NN denotes phenotype
T9595 11041-11046 JJ denotes overt
T9596 11057-11059 IN denotes in
T9597 11060-11066 NN denotes mutant
T9598 11067-11071 NNS denotes mice
T9599 11071-11073 , denotes ,
T9600 11073-11077 IN denotes save
T9601 11078-11087 JJ denotes distended
T9602 11088-11095 NNS denotes kidneys
T9603 11095-11097 , denotes ,
T9604 11097-11102 WDT denotes which
T9605 11103-11111 VBD denotes appeared
T9606 11112-11120 RB denotes variably
T9607 11121-11123 IN denotes in
T9608 11124-11129 JJ denotes adult
T9609 11130-11137 NNS denotes animals
T9610 11138-11139 -LRB- denotes (
T9611 11139-11145 NN denotes Figure
T9612 11146-11148 CD denotes 2A
T9613 11148-11149 -RRB- denotes )
T9614 11149-11150 . denotes .
T9615 11150-11230 sentence denotes Although not specifically measured, mutant mice seem to have a normal lifespan.
T9616 11151-11159 IN denotes Although
T9617 11177-11185 VBN denotes measured
T9618 11160-11163 RB denotes not
T9619 11164-11176 RB denotes specifically
T9620 11199-11203 VBP denotes seem
T9621 11185-11187 , denotes ,
T9622 11187-11193 NN denotes mutant
T9623 11194-11198 NNS denotes mice
T9624 11204-11206 TO denotes to
T9625 11207-11211 VB denotes have
T9626 11212-11213 DT denotes a
T9627 11221-11229 NN denotes lifespan
T9628 11214-11220 JJ denotes normal
T9629 11229-11230 . denotes .
T9630 11230-11314 sentence denotes The one animal that was followed lived to 18 mo, typical for animals in our colony.
T9631 11231-11234 DT denotes The
T9632 11239-11245 NN denotes animal
T9633 11235-11238 CD denotes one
T9634 11264-11269 VBN denotes lived
T9635 11246-11250 WDT denotes that
T9636 11255-11263 VBN denotes followed
T9637 11251-11254 VBD denotes was
T9638 11270-11272 IN denotes to
T9639 11273-11275 CD denotes 18
T9640 11276-11278 NNS denotes mo
T9641 11278-11280 , denotes ,
T9642 11280-11287 JJ denotes typical
T9643 11288-11291 IN denotes for
T9644 11292-11299 NNS denotes animals
T9645 11300-11302 IN denotes in
T9646 11303-11306 PRP$ denotes our
T9647 11307-11313 NN denotes colony
T9648 11313-11314 . denotes .
T9649 11314-12312 sentence denotes Figure 2 Anatomy and Histology of Mouse Kidneys (A) Gross anatomy of an affected mouse (8-mo-old male). This shows the enlargement and cystic dilatation of the renal pelvis. There is thinning of the overlying renal parenchyma imparting a translucent appearance to portions of the kidney and collecting system. The bladder is also dilated. (B) Left kidney from mutant mouse (right) shown in (A) compared to a kidney from an age-sex matched unaffected littermate (left). (C) Hematoxylin and eosin stained section of ureter from a mutant mouse, showing normal histology despite bloating of the kidney. (D) Hematoxylin and eosin stained histologic section of a kidney from a 4-wk-old female mutant mouse. The mutant kidney shows marked dilatation of the renal pelvis with blunting of the papilla. There is preservation of the cortex and medulla. Aqp2F204V/F204V mice suffer from severe hydronephrosis (Figure 2A and 2B), presumably as a consequence of an inability to cope with the extreme polyuria.
T9650 12159-12168 NN denotes Aqp2F204V
T9651 12169-12174 NN denotes F204V
T9652 12168-12169 HYPH denotes /
T9653 12175-12179 NNS denotes mice
T9654 12180-12186 VBP denotes suffer
T9655 12187-12191 IN denotes from
T9656 12192-12198 JJ denotes severe
T9657 12199-12213 NN denotes hydronephrosis
T9658 12214-12215 -LRB- denotes (
T9659 12222-12224 CD denotes 2A
T9660 12215-12221 NN denotes Figure
T9661 12225-12228 CC denotes and
T9662 12229-12231 CD denotes 2B
T9663 12231-12232 -RRB- denotes )
T9664 12232-12234 , denotes ,
T9665 12234-12244 RB denotes presumably
T9666 12245-12247 IN denotes as
T9667 12248-12249 DT denotes a
T9668 12250-12261 NN denotes consequence
T9669 12262-12264 IN denotes of
T9670 12265-12267 DT denotes an
T9671 12268-12277 NN denotes inability
T9672 12278-12280 TO denotes to
T9673 12281-12285 VB denotes cope
T9674 12286-12290 IN denotes with
T9675 12291-12294 DT denotes the
T9676 12303-12311 NN denotes polyuria
T9677 12295-12302 JJ denotes extreme
T9678 12311-12312 . denotes .
T9679 12312-12454 sentence denotes We found distended kidneys in all Aqp2F204V/F204V mice; however, the degree of inflation was variable in affected mice and worsened with age.
T9680 12313-12315 PRP denotes We
T9681 12316-12321 VBD denotes found
T9682 12402-12405 VBD denotes was
T9683 12322-12331 JJ denotes distended
T9684 12332-12339 NNS denotes kidneys
T9685 12340-12342 IN denotes in
T9686 12343-12346 DT denotes all
T9687 12363-12367 NNS denotes mice
T9688 12347-12356 NN denotes Aqp2F204V
T9689 12357-12362 NN denotes F204V
T9690 12356-12357 HYPH denotes /
T9691 12367-12368 : denotes ;
T9692 12369-12376 RB denotes however
T9693 12376-12378 , denotes ,
T9694 12378-12381 DT denotes the
T9695 12382-12388 NN denotes degree
T9696 12389-12391 IN denotes of
T9697 12392-12401 NN denotes inflation
T9698 12406-12414 JJ denotes variable
T9699 12415-12417 IN denotes in
T9700 12418-12426 VBN denotes affected
T9701 12427-12431 NNS denotes mice
T9702 12432-12435 CC denotes and
T9703 12436-12444 VBD denotes worsened
T9704 12445-12449 IN denotes with
T9705 12450-12453 NN denotes age
T9706 12453-12454 . denotes .
T9707 12454-12567 sentence denotes Severe hydronephrosis has previously been observed in double Aqp1/Aqp3 knock-out mice [17], and appears at 6 wk.
T9708 12455-12461 JJ denotes Severe
T9709 12462-12476 NN denotes hydronephrosis
T9710 12497-12505 VBN denotes observed
T9711 12477-12480 VBZ denotes has
T9712 12481-12491 RB denotes previously
T9713 12492-12496 VBN denotes been
T9714 12506-12508 IN denotes in
T9715 12509-12515 JJ denotes double
T9716 12526-12531 VB denotes knock
T9717 12516-12520 NN denotes Aqp1
T9718 12521-12525 NN denotes Aqp3
T9719 12520-12521 HYPH denotes /
T9720 12536-12540 NNS denotes mice
T9721 12531-12532 HYPH denotes -
T9722 12532-12535 RP denotes out
T9723 12541-12542 -LRB- denotes [
T9724 12542-12544 CD denotes 17
T9725 12544-12545 -RRB- denotes ]
T9726 12545-12547 , denotes ,
T9727 12547-12550 CC denotes and
T9728 12551-12558 VBZ denotes appears
T9729 12559-12561 IN denotes at
T9730 12562-12563 CD denotes 6
T9731 12564-12566 NNS denotes wk
T9732 12566-12567 . denotes .
T9733 12567-12622 sentence denotes Even at 4 wk, Aqp2F204V/F204V mice had hydronephrosis.
T9734 12568-12572 RB denotes Even
T9735 12573-12575 IN denotes at
T9736 12603-12606 VBD denotes had
T9737 12576-12577 CD denotes 4
T9738 12578-12580 NNS denotes wk
T9739 12580-12582 , denotes ,
T9740 12582-12591 NN denotes Aqp2F204V
T9741 12592-12597 NN denotes F204V
T9742 12591-12592 HYPH denotes /
T9743 12598-12602 NNS denotes mice
T9744 12607-12621 NN denotes hydronephrosis
T9745 12621-12622 . denotes .
T9746 12622-12775 sentence denotes Histologic sections from Aqp2F204V/F204V mice demonstrated marked dilatation of the renal pelvis yet normal morphology of the ureter (Figure 2C and 2D).
T9747 12623-12633 JJ denotes Histologic
T9748 12634-12642 NNS denotes sections
T9749 12669-12681 VBD denotes demonstrated
T9750 12643-12647 IN denotes from
T9751 12648-12657 NN denotes Aqp2F204V
T9752 12658-12663 NN denotes F204V
T9753 12657-12658 HYPH denotes /
T9754 12664-12668 NNS denotes mice
T9755 12682-12688 JJ denotes marked
T9756 12689-12699 NN denotes dilatation
T9757 12700-12702 IN denotes of
T9758 12703-12706 DT denotes the
T9759 12713-12719 NN denotes pelvis
T9760 12707-12712 JJ denotes renal
T9761 12720-12723 CC denotes yet
T9762 12724-12730 JJ denotes normal
T9763 12731-12741 NN denotes morphology
T9764 12742-12744 IN denotes of
T9765 12745-12748 DT denotes the
T9766 12749-12755 NN denotes ureter
T9767 12756-12757 -LRB- denotes (
T9768 12764-12766 CD denotes 2C
T9769 12757-12763 NN denotes Figure
T9770 12767-12770 CC denotes and
T9771 12771-12773 CD denotes 2D
T9772 12773-12774 -RRB- denotes )
T9773 12774-12775 . denotes .
T9774 12775-12852 sentence denotes In particular, the muscularis propria was neither hypertrophied nor thinned.
T9775 12776-12778 IN denotes In
T9776 12814-12817 VBD denotes was
T9777 12779-12789 JJ denotes particular
T9778 12789-12791 , denotes ,
T9779 12791-12794 DT denotes the
T9780 12806-12813 NN denotes propria
T9781 12795-12805 NN denotes muscularis
T9782 12818-12825 CC denotes neither
T9783 12826-12839 JJ denotes hypertrophied
T9784 12840-12843 CC denotes nor
T9785 12844-12851 JJ denotes thinned
T9786 12851-12852 . denotes .
T9787 12852-12971 sentence denotes There was the normal festooned appearance of the urothelium, and this transitional epithelium was of normal thickness.
T9788 12853-12858 EX denotes There
T9789 12859-12862 VBD denotes was
T9790 12863-12866 DT denotes the
T9791 12884-12894 NN denotes appearance
T9792 12867-12873 JJ denotes normal
T9793 12874-12883 VBN denotes festooned
T9794 12895-12897 IN denotes of
T9795 12898-12901 DT denotes the
T9796 12902-12912 NN denotes urothelium
T9797 12912-12914 , denotes ,
T9798 12914-12917 CC denotes and
T9799 12918-12922 DT denotes this
T9800 12936-12946 NN denotes epithelium
T9801 12923-12935 JJ denotes transitional
T9802 12947-12950 VBD denotes was
T9803 12951-12953 IN denotes of
T9804 12954-12960 JJ denotes normal
T9805 12961-12970 NN denotes thickness
T9806 12970-12971 . denotes .
T9807 12971-13052 sentence denotes There was thinning of the kidney as measured from renal capsule to renal pelvis.
T9808 12972-12977 EX denotes There
T9809 12978-12981 VBD denotes was
T9810 12982-12990 NN denotes thinning
T9811 12991-12993 IN denotes of
T9812 12994-12997 DT denotes the
T9813 12998-13004 NN denotes kidney
T9814 13005-13007 IN denotes as
T9815 13008-13016 VBN denotes measured
T9816 13017-13021 IN denotes from
T9817 13022-13027 JJ denotes renal
T9818 13028-13035 NN denotes capsule
T9819 13036-13038 IN denotes to
T9820 13039-13044 JJ denotes renal
T9821 13045-13051 NN denotes pelvis
T9822 13051-13052 . denotes .
T9823 13052-13162 sentence denotes However, the morphologic features of the glomeruli and proximal/distal tubules were unremarkable (Figure 2D).
T9824 13053-13060 RB denotes However
T9825 13132-13136 VBD denotes were
T9826 13060-13062 , denotes ,
T9827 13062-13065 DT denotes the
T9828 13078-13086 NNS denotes features
T9829 13066-13077 JJ denotes morphologic
T9830 13087-13089 IN denotes of
T9831 13090-13093 DT denotes the
T9832 13094-13103 NNS denotes glomeruli
T9833 13104-13107 CC denotes and
T9834 13108-13116 JJ denotes proximal
T9835 13117-13123 JJ denotes distal
T9836 13116-13117 HYPH denotes /
T9837 13124-13131 NNS denotes tubules
T9838 13137-13149 JJ denotes unremarkable
T9839 13150-13151 -LRB- denotes (
T9840 13151-13157 NN denotes Figure
T9841 13158-13160 CD denotes 2D
T9842 13160-13161 -RRB- denotes )
T9843 13161-13162 . denotes .
T9844 13162-13311 sentence denotes As shown previously [18,22], immunoblotting revealed three different forms of AQP2, due to different degrees and forms of glycosylation (Figure 3A).
T9845 13163-13165 IN denotes As
T9846 13166-13171 VBN denotes shown
T9847 13207-13215 VBD denotes revealed
T9848 13172-13182 RB denotes previously
T9849 13183-13184 -LRB- denotes [
T9850 13187-13189 CD denotes 22
T9851 13184-13186 CD denotes 18
T9852 13186-13187 , denotes ,
T9853 13189-13190 -RRB- denotes ]
T9854 13190-13192 , denotes ,
T9855 13192-13206 NN denotes immunoblotting
T9856 13216-13221 CD denotes three
T9857 13232-13237 NNS denotes forms
T9858 13222-13231 JJ denotes different
T9859 13238-13240 IN denotes of
T9860 13241-13245 NN denotes AQP2
T9861 13245-13247 , denotes ,
T9862 13247-13250 IN denotes due
T9863 13251-13253 IN denotes to
T9864 13254-13263 JJ denotes different
T9865 13264-13271 NNS denotes degrees
T9866 13272-13275 CC denotes and
T9867 13276-13281 NNS denotes forms
T9868 13282-13284 IN denotes of
T9869 13285-13298 NN denotes glycosylation
T9870 13299-13300 -LRB- denotes (
T9871 13300-13306 NN denotes Figure
T9872 13307-13309 CD denotes 3A
T9873 13309-13310 -RRB- denotes )
T9874 13310-13311 . denotes .
T9875 13311-13475 sentence denotes Previous reports have demonstrated that nonglycosylated protein appears as a 29 kDa band, while complex glycosylated protein runs as a smear between 35 and 45 kDa.
T9876 13312-13320 JJ denotes Previous
T9877 13321-13328 NNS denotes reports
T9878 13334-13346 VBN denotes demonstrated
T9879 13329-13333 VBP denotes have
T9880 13347-13351 IN denotes that
T9881 13376-13383 VBZ denotes appears
T9882 13352-13367 JJ denotes nonglycosylated
T9883 13368-13375 NN denotes protein
T9884 13384-13386 IN denotes as
T9885 13387-13388 DT denotes a
T9886 13396-13400 NN denotes band
T9887 13389-13391 CD denotes 29
T9888 13392-13395 NN denotes kDa
T9889 13400-13402 , denotes ,
T9890 13402-13407 IN denotes while
T9891 13437-13441 VBZ denotes runs
T9892 13408-13415 JJ denotes complex
T9893 13429-13436 NN denotes protein
T9894 13416-13428 VBN denotes glycosylated
T9895 13442-13444 IN denotes as
T9896 13445-13446 DT denotes a
T9897 13447-13452 NN denotes smear
T9898 13453-13460 IN denotes between
T9899 13461-13463 CD denotes 35
T9900 13471-13474 NN denotes kDa
T9901 13464-13467 CC denotes and
T9902 13468-13470 CD denotes 45
T9903 13474-13475 . denotes .
T9904 13475-13627 sentence denotes A short-lived intermediate form of 31 kDa representing core, high-mannose glycosylation of AQP2 is apparent from pulse-chase labeling experiments [22].
T9905 13476-13477 DT denotes A
T9906 13503-13507 NN denotes form
T9907 13478-13483 JJ denotes short
T9908 13484-13489 JJ denotes lived
T9909 13483-13484 HYPH denotes -
T9910 13490-13502 JJ denotes intermediate
T9911 13572-13574 VBZ denotes is
T9912 13508-13510 IN denotes of
T9913 13511-13513 CD denotes 31
T9914 13514-13517 NN denotes kDa
T9915 13518-13530 VBG denotes representing
T9916 13531-13535 NN denotes core
T9917 13550-13563 NN denotes glycosylation
T9918 13535-13537 , denotes ,
T9919 13537-13541 JJ denotes high
T9920 13542-13549 NN denotes mannose
T9921 13541-13542 HYPH denotes -
T9922 13564-13566 IN denotes of
T9923 13567-13571 NN denotes AQP2
T9924 13575-13583 JJ denotes apparent
T9925 13584-13588 IN denotes from
T9926 13589-13594 NN denotes pulse
T9927 13595-13600 NN denotes chase
T9928 13594-13595 HYPH denotes -
T9929 13601-13609 NN denotes labeling
T9930 13610-13621 NNS denotes experiments
T9931 13622-13623 -LRB- denotes [
T9932 13623-13625 CD denotes 22
T9933 13625-13626 -RRB- denotes ]
T9934 13626-13627 . denotes .
T9935 13627-13874 sentence denotes Compared to that from the kidneys of wild-type animals, AQP2 from mutant animals was reduced in both the high molecular weight, diffuse form and the lowest molecular weight form, but enriched in the intermediate molecular weight form (Figure 3A).
T9936 13628-13636 VBN denotes Compared
T9937 13713-13720 VBN denotes reduced
T9938 13637-13639 IN denotes to
T9939 13640-13644 DT denotes that
T9940 13645-13649 IN denotes from
T9941 13650-13653 DT denotes the
T9942 13654-13661 NNS denotes kidneys
T9943 13662-13664 IN denotes of
T9944 13665-13669 JJ denotes wild
T9945 13670-13674 NN denotes type
T9946 13669-13670 HYPH denotes -
T9947 13675-13682 NNS denotes animals
T9948 13682-13684 , denotes ,
T9949 13684-13688 NN denotes AQP2
T9950 13689-13693 IN denotes from
T9951 13694-13700 NN denotes mutant
T9952 13701-13708 NNS denotes animals
T9953 13709-13712 VBD denotes was
T9954 13721-13723 IN denotes in
T9955 13724-13728 CC denotes both
T9956 13764-13768 NN denotes form
T9957 13729-13732 DT denotes the
T9958 13733-13737 JJ denotes high
T9959 13748-13754 NN denotes weight
T9960 13738-13747 JJ denotes molecular
T9961 13754-13756 , denotes ,
T9962 13756-13763 JJ denotes diffuse
T9963 13769-13772 CC denotes and
T9964 13773-13776 DT denotes the
T9965 13801-13805 NN denotes form
T9966 13777-13783 JJS denotes lowest
T9967 13794-13800 NN denotes weight
T9968 13784-13793 JJ denotes molecular
T9969 13805-13807 , denotes ,
T9970 13807-13810 CC denotes but
T9971 13811-13819 VBN denotes enriched
T9972 13820-13822 IN denotes in
T9973 13823-13826 DT denotes the
T9974 13857-13861 NN denotes form
T9975 13827-13839 JJ denotes intermediate
T9976 13850-13856 NN denotes weight
T9977 13840-13849 JJ denotes molecular
T9978 13862-13863 -LRB- denotes (
T9979 13863-13869 NN denotes Figure
T9980 13870-13872 CD denotes 3A
T9981 13872-13873 -RRB- denotes )
T9982 13873-13874 . denotes .
T9983 13874-13943 sentence denotes Heterozygous animals showed intermediate amounts of all three forms.
T9984 13875-13887 JJ denotes Heterozygous
T9985 13888-13895 NNS denotes animals
T9986 13896-13902 VBD denotes showed
T9987 13903-13915 JJ denotes intermediate
T9988 13916-13923 NNS denotes amounts
T9989 13924-13926 IN denotes of
T9990 13927-13930 DT denotes all
T9991 13937-13942 NNS denotes forms
T9992 13931-13936 CD denotes three
T9993 13942-13943 . denotes .
T9994 13943-14112 sentence denotes The nature of these glycosylated forms was revealed by digestion with endoglycosidase H, which specifically cleaves mannose-rich carbohydrate from the protein backbone.
T9995 13944-13947 DT denotes The
T9996 13948-13954 NN denotes nature
T9997 13987-13995 VBN denotes revealed
T9998 13955-13957 IN denotes of
T9999 13958-13963 DT denotes these
T10000 13977-13982 NNS denotes forms
T10001 13964-13976 VBN denotes glycosylated
T10002 13983-13986 VBD denotes was
T10003 13996-13998 IN denotes by
T10004 13999-14008 NN denotes digestion
T10005 14009-14013 IN denotes with
T10006 14014-14029 NN denotes endoglycosidase
T10007 14030-14031 NN denotes H
T10008 14031-14033 , denotes ,
T10009 14033-14038 WDT denotes which
T10010 14052-14059 VBZ denotes cleaves
T10011 14039-14051 RB denotes specifically
T10012 14060-14067 NN denotes mannose
T10013 14068-14072 JJ denotes rich
T10014 14067-14068 HYPH denotes -
T10015 14073-14085 NN denotes carbohydrate
T10016 14086-14090 IN denotes from
T10017 14091-14094 DT denotes the
T10018 14103-14111 NN denotes backbone
T10019 14095-14102 NN denotes protein
T10020 14111-14112 . denotes .
T10021 14112-14275 sentence denotes Treatment of endogenous AQP2 from kidneys of wild-type, heterozygous, and mutant animals specifically affected the intermediate molecular weight form (Figure 3B).
T10022 14113-14122 NN denotes Treatment
T10023 14215-14223 VBD denotes affected
T10024 14123-14125 IN denotes of
T10025 14126-14136 JJ denotes endogenous
T10026 14137-14141 NN denotes AQP2
T10027 14142-14146 IN denotes from
T10028 14147-14154 NNS denotes kidneys
T10029 14155-14157 IN denotes of
T10030 14158-14162 JJ denotes wild
T10031 14163-14167 NN denotes type
T10032 14162-14163 HYPH denotes -
T10033 14194-14201 NNS denotes animals
T10034 14167-14169 , denotes ,
T10035 14169-14181 JJ denotes heterozygous
T10036 14181-14183 , denotes ,
T10037 14183-14186 CC denotes and
T10038 14187-14193 JJ denotes mutant
T10039 14202-14214 RB denotes specifically
T10040 14224-14227 DT denotes the
T10041 14258-14262 NN denotes form
T10042 14228-14240 JJ denotes intermediate
T10043 14251-14257 NN denotes weight
T10044 14241-14250 JJ denotes molecular
T10045 14263-14264 -LRB- denotes (
T10046 14264-14270 NN denotes Figure
T10047 14271-14273 CD denotes 3B
T10048 14273-14274 -RRB- denotes )
T10049 14274-14275 . denotes .
T10050 14275-14484 sentence denotes The presence of some mature glycosylated proteins (35–45 kDa) in Aqp2F204V/F204V mice presumably permits their survival compared to Aqp2T126M/T126M mice, and is consistent with a diminished response to dDAVP.
T10051 14276-14279 DT denotes The
T10052 14280-14288 NN denotes presence
T10053 14373-14380 VBZ denotes permits
T10054 14289-14291 IN denotes of
T10055 14292-14296 DT denotes some
T10056 14317-14325 NN denotes proteins
T10057 14297-14303 JJ denotes mature
T10058 14304-14316 VBN denotes glycosylated
T10059 14326-14327 -LRB- denotes (
T10060 14333-14336 NN denotes kDa
T10061 14327-14329 CD denotes 35
T10062 14330-14332 CD denotes 45
T10063 14329-14330 SYM denotes –
T10064 14336-14337 -RRB- denotes )
T10065 14338-14340 IN denotes in
T10066 14341-14350 NN denotes Aqp2F204V
T10067 14351-14356 NN denotes F204V
T10068 14350-14351 HYPH denotes /
T10069 14357-14361 NNS denotes mice
T10070 14362-14372 RB denotes presumably
T10071 14381-14386 PRP$ denotes their
T10072 14387-14395 NN denotes survival
T10073 14396-14404 VBN denotes compared
T10074 14405-14407 IN denotes to
T10075 14408-14417 NN denotes Aqp2T126M
T10076 14418-14423 NN denotes T126M
T10077 14417-14418 HYPH denotes /
T10078 14424-14428 NNS denotes mice
T10079 14428-14430 , denotes ,
T10080 14430-14433 CC denotes and
T10081 14434-14436 VBZ denotes is
T10082 14437-14447 JJ denotes consistent
T10083 14448-14452 IN denotes with
T10084 14453-14454 DT denotes a
T10085 14466-14474 NN denotes response
T10086 14455-14465 JJ denotes diminished
T10087 14475-14477 IN denotes to
T10088 14478-14483 NN denotes dDAVP
T10089 14483-14484 . denotes .
T10090 14484-15138 sentence denotes Figure 3 Immunoblot Analyses of AQP2 from Mouse Kidneys (A) Western blot analyses of total kidney membranes from littermate mice. An intermediate form of AQP2 at 31 kDa was identified in kidney membranes from a mutant mouse (Mut) and partially in a heterozygous mouse (Het). (B) Total kidney membranes were subjected to endoglycosidase H treatment (Endo H) prior to Western blotting. High-mannose (h.m.) glycosylated proteins that have not exited the ER are sensitive to endoglycosidase H digestion. In humans, recessive alleles of Aqp2 are postulated to cause NDI because they do not properly translocate to the apical cell surface in response to AVP.
T10091 14986-14988 IN denotes In
T10092 15027-15037 VBN denotes postulated
T10093 14989-14995 NNS denotes humans
T10094 14995-14997 , denotes ,
T10095 14997-15006 JJ denotes recessive
T10096 15007-15014 NNS denotes alleles
T10097 15015-15017 IN denotes of
T10098 15018-15022 NN denotes Aqp2
T10099 15023-15026 VBP denotes are
T10100 15038-15040 TO denotes to
T10101 15041-15046 VB denotes cause
T10102 15047-15050 NN denotes NDI
T10103 15051-15058 IN denotes because
T10104 15080-15091 VB denotes translocate
T10105 15059-15063 PRP denotes they
T10106 15064-15066 VBP denotes do
T10107 15067-15070 RB denotes not
T10108 15071-15079 RB denotes properly
T10109 15092-15094 IN denotes to
T10110 15095-15098 DT denotes the
T10111 15111-15118 NN denotes surface
T10112 15099-15105 JJ denotes apical
T10113 15106-15110 NN denotes cell
T10114 15119-15121 IN denotes in
T10115 15122-15130 NN denotes response
T10116 15131-15133 IN denotes to
T10117 15134-15137 NN denotes AVP
T10118 15137-15138 . denotes .
T10119 15138-15296 sentence denotes This postulate comes solely from in vitro studies in which mutant Aqp2 cDNAs corresponding to human disease mutations are transfected into kidney cell lines.
T10120 15139-15143 DT denotes This
T10121 15144-15153 NN denotes postulate
T10122 15154-15159 VBZ denotes comes
T10123 15160-15166 RB denotes solely
T10124 15167-15171 IN denotes from
T10125 15172-15174 FW denotes in
T10126 15175-15180 FW denotes vitro
T10127 15181-15188 NNS denotes studies
T10128 15189-15191 IN denotes in
T10129 15261-15272 VBN denotes transfected
T10130 15192-15197 WDT denotes which
T10131 15198-15204 NN denotes mutant
T10132 15210-15215 NNS denotes cDNAs
T10133 15205-15209 NN denotes Aqp2
T10134 15216-15229 VBG denotes corresponding
T10135 15230-15232 IN denotes to
T10136 15233-15238 JJ denotes human
T10137 15247-15256 NNS denotes mutations
T10138 15239-15246 NN denotes disease
T10139 15257-15260 VBP denotes are
T10140 15273-15277 IN denotes into
T10141 15278-15284 NN denotes kidney
T10142 15290-15295 NNS denotes lines
T10143 15285-15289 NN denotes cell
T10144 15295-15296 . denotes .
T10145 15296-15415 sentence denotes In general, such recessive alleles, when visualized immunocytochemically, fail to localize to AVP-responsive vesicles.
T10146 15297-15299 IN denotes In
T10147 15371-15375 VBP denotes fail
T10148 15300-15307 JJ denotes general
T10149 15307-15309 , denotes ,
T10150 15309-15313 JJ denotes such
T10151 15324-15331 NNS denotes alleles
T10152 15314-15323 JJ denotes recessive
T10153 15331-15333 , denotes ,
T10154 15333-15337 WRB denotes when
T10155 15338-15348 VBN denotes visualized
T10156 15349-15369 RB denotes immunocytochemically
T10157 15369-15371 , denotes ,
T10158 15376-15378 TO denotes to
T10159 15379-15387 VB denotes localize
T10160 15388-15390 IN denotes to
T10161 15391-15394 NN denotes AVP
T10162 15395-15405 JJ denotes responsive
T10163 15394-15395 HYPH denotes -
T10164 15406-15414 NNS denotes vesicles
T10165 15414-15415 . denotes .
T10166 15415-15475 sentence denotes Rather, they get trapped in the endoplasmic reticulum (ER).
T10167 15416-15422 RB denotes Rather
T10168 15433-15440 VBN denotes trapped
T10169 15422-15424 , denotes ,
T10170 15424-15428 PRP denotes they
T10171 15429-15432 VBP denotes get
T10172 15441-15443 IN denotes in
T10173 15444-15447 DT denotes the
T10174 15460-15469 NN denotes reticulum
T10175 15448-15459 JJ denotes endoplasmic
T10176 15470-15471 -LRB- denotes (
T10177 15471-15473 NN denotes ER
T10178 15473-15474 -RRB- denotes )
T10179 15474-15475 . denotes .
T10180 15475-15572 sentence denotes Our mouse model of NDI affords the first opportunity to test this hypothesis in a mature animal.
T10181 15476-15479 PRP$ denotes Our
T10182 15486-15491 NN denotes model
T10183 15480-15485 NN denotes mouse
T10184 15499-15506 VBZ denotes affords
T10185 15492-15494 IN denotes of
T10186 15495-15498 NN denotes NDI
T10187 15507-15510 DT denotes the
T10188 15517-15528 NN denotes opportunity
T10189 15511-15516 JJ denotes first
T10190 15529-15531 TO denotes to
T10191 15532-15536 VB denotes test
T10192 15537-15541 DT denotes this
T10193 15542-15552 NN denotes hypothesis
T10194 15553-15555 IN denotes in
T10195 15556-15557 DT denotes a
T10196 15565-15571 NN denotes animal
T10197 15558-15564 JJ denotes mature
T10198 15571-15572 . denotes .
T10199 15572-15742 sentence denotes As shown in Figure 4A (top row of photomicrographs), AQP2 (stained red) normally localized to the subapical region of collecting duct cells in kidneys of wild-type mice.
T10200 15573-15575 IN denotes As
T10201 15576-15581 VBN denotes shown
T10202 15654-15663 VBD denotes localized
T10203 15582-15584 IN denotes in
T10204 15585-15591 NN denotes Figure
T10205 15592-15594 CD denotes 4A
T10206 15595-15596 -LRB- denotes (
T10207 15600-15603 NN denotes row
T10208 15596-15599 JJ denotes top
T10209 15604-15606 IN denotes of
T10210 15607-15623 NNS denotes photomicrographs
T10211 15623-15624 -RRB- denotes )
T10212 15624-15626 , denotes ,
T10213 15626-15630 NN denotes AQP2
T10214 15631-15632 -LRB- denotes (
T10215 15632-15639 VBN denotes stained
T10216 15640-15643 JJ denotes red
T10217 15643-15644 -RRB- denotes )
T10218 15645-15653 RB denotes normally
T10219 15664-15666 IN denotes to
T10220 15667-15670 DT denotes the
T10221 15681-15687 NN denotes region
T10222 15671-15680 JJ denotes subapical
T10223 15688-15690 IN denotes of
T10224 15691-15701 VBG denotes collecting
T10225 15702-15706 NN denotes duct
T10226 15707-15712 NNS denotes cells
T10227 15713-15715 IN denotes in
T10228 15716-15723 NNS denotes kidneys
T10229 15724-15726 IN denotes of
T10230 15727-15731 JJ denotes wild
T10231 15732-15736 NN denotes type
T10232 15731-15732 HYPH denotes -
T10233 15737-15741 NNS denotes mice
T10234 15741-15742 . denotes .
T10235 15742-15842 sentence denotes Upon stimulation with dDAVP, AQP2 translocated to or near the cell surface (Figure 4A, second row).
T10236 15743-15747 IN denotes Upon
T10237 15777-15789 VBD denotes translocated
T10238 15748-15759 NN denotes stimulation
T10239 15760-15764 IN denotes with
T10240 15765-15770 NN denotes dDAVP
T10241 15770-15772 , denotes ,
T10242 15772-15776 NN denotes AQP2
T10243 15790-15792 IN denotes to
T10244 15793-15795 CC denotes or
T10245 15796-15800 IN denotes near
T10246 15801-15804 DT denotes the
T10247 15810-15817 NN denotes surface
T10248 15805-15809 NN denotes cell
T10249 15818-15819 -LRB- denotes (
T10250 15837-15840 NN denotes row
T10251 15819-15825 NN denotes Figure
T10252 15826-15828 CD denotes 4A
T10253 15828-15830 , denotes ,
T10254 15830-15836 JJ denotes second
T10255 15840-15841 -RRB- denotes )
T10256 15841-15842 . denotes .
T10257 15842-16058 sentence denotes In kidneys taken from mutant animals, however, AQP2 was distributed randomly throughout the cell in the basal state (Figure 4A, third row), while AQP3 (green) appropriately localized to the basolateral surface [23].
T10258 15843-15845 IN denotes In
T10259 15899-15910 VBN denotes distributed
T10260 15846-15853 NNS denotes kidneys
T10261 15854-15859 VBN denotes taken
T10262 15860-15864 IN denotes from
T10263 15865-15871 NN denotes mutant
T10264 15872-15879 NNS denotes animals
T10265 15879-15881 , denotes ,
T10266 15881-15888 RB denotes however
T10267 15888-15890 , denotes ,
T10268 15890-15894 NN denotes AQP2
T10269 15895-15898 VBD denotes was
T10270 15911-15919 RB denotes randomly
T10271 15920-15930 IN denotes throughout
T10272 15931-15934 DT denotes the
T10273 15935-15939 NN denotes cell
T10274 15940-15942 IN denotes in
T10275 15943-15946 DT denotes the
T10276 15953-15958 NN denotes state
T10277 15947-15952 JJ denotes basal
T10278 15959-15960 -LRB- denotes (
T10279 15977-15980 NN denotes row
T10280 15960-15966 NN denotes Figure
T10281 15967-15969 CD denotes 4A
T10282 15969-15971 , denotes ,
T10283 15971-15976 JJ denotes third
T10284 15980-15981 -RRB- denotes )
T10285 15981-15983 , denotes ,
T10286 15983-15988 IN denotes while
T10287 16016-16025 VBD denotes localized
T10288 15989-15993 NN denotes AQP3
T10289 15994-15995 -LRB- denotes (
T10290 15995-16000 NN denotes green
T10291 16000-16001 -RRB- denotes )
T10292 16002-16015 RB denotes appropriately
T10293 16026-16028 IN denotes to
T10294 16029-16032 DT denotes the
T10295 16045-16052 NN denotes surface
T10296 16033-16044 JJ denotes basolateral
T10297 16053-16054 -LRB- denotes [
T10298 16054-16056 CD denotes 23
T10299 16056-16057 -RRB- denotes ]
T10300 16057-16058 . denotes .
T10301 16058-16173 sentence denotes Furthermore, upon dDAVP stimulation, AQP2-F204V failed to translocate to the cell surface (Figure 4A, bottom row).
T10302 16059-16070 RB denotes Furthermore
T10303 16107-16113 VBD denotes failed
T10304 16070-16072 , denotes ,
T10305 16072-16076 IN denotes upon
T10306 16077-16082 NN denotes dDAVP
T10307 16083-16094 NN denotes stimulation
T10308 16094-16096 , denotes ,
T10309 16096-16100 NN denotes AQP2
T10310 16101-16106 NN denotes F204V
T10311 16100-16101 HYPH denotes -
T10312 16114-16116 TO denotes to
T10313 16117-16128 VB denotes translocate
T10314 16129-16131 IN denotes to
T10315 16132-16135 DT denotes the
T10316 16141-16148 NN denotes surface
T10317 16136-16140 NN denotes cell
T10318 16149-16150 -LRB- denotes (
T10319 16168-16171 NN denotes row
T10320 16150-16156 NN denotes Figure
T10321 16157-16159 CD denotes 4A
T10322 16159-16161 , denotes ,
T10323 16161-16167 JJ denotes bottom
T10324 16171-16172 -RRB- denotes )
T10325 16172-16173 . denotes .
T10326 16173-16359 sentence denotes To confirm these findings, the staining was repeated in kidneys taken from two further mice for each class, wild-type or mutant, with or without dDAVP treatment, with identical results.
T10327 16174-16176 TO denotes To
T10328 16177-16184 VB denotes confirm
T10329 16218-16226 VBN denotes repeated
T10330 16185-16190 DT denotes these
T10331 16191-16199 NNS denotes findings
T10332 16199-16201 , denotes ,
T10333 16201-16204 DT denotes the
T10334 16205-16213 NN denotes staining
T10335 16214-16217 VBD denotes was
T10336 16227-16229 IN denotes in
T10337 16230-16237 NNS denotes kidneys
T10338 16238-16243 VBN denotes taken
T10339 16244-16248 IN denotes from
T10340 16249-16252 CD denotes two
T10341 16261-16265 NNS denotes mice
T10342 16253-16260 JJ denotes further
T10343 16266-16269 IN denotes for
T10344 16270-16274 DT denotes each
T10345 16275-16280 NN denotes class
T10346 16280-16282 , denotes ,
T10347 16282-16286 JJ denotes wild
T10348 16287-16291 NN denotes type
T10349 16286-16287 HYPH denotes -
T10350 16292-16294 CC denotes or
T10351 16295-16301 NN denotes mutant
T10352 16301-16303 , denotes ,
T10353 16303-16307 IN denotes with
T10354 16308-16310 CC denotes or
T10355 16311-16318 IN denotes without
T10356 16319-16324 NN denotes dDAVP
T10357 16325-16334 NN denotes treatment
T10358 16334-16336 , denotes ,
T10359 16336-16340 IN denotes with
T10360 16341-16350 JJ denotes identical
T10361 16351-16358 NNS denotes results
T10362 16358-16359 . denotes .
T10363 16359-18139 sentence denotes Figure 4 AQP2 Subcellular Localization and Translocation in Mouse Kidney Collecting Ducts and MDCK Cell Lines (A) Immunohistochemistry on collecting ducts in kidney sections from an AQP2-F204V mutant (Mut) mouse and an age-sex matched wild-type (WT) littermate. Mice were injected intraperitoneally with PBS (NT) or dDAVP before sacrificing and fixation of the kidneys. Kidneys sections were immunostained for AQP2 (red) and the basolateral marker AQP3 (green). The images were merged and an area of the cytoplasm was magnified (zoom). Note that mutant AQP2 is not properly localized to the subapical compartment, nor does it respond to dDAVP. (B) MDCK cell lines, stably transfected with constructs encoding mouse WT or AQP2-F204V, were treated with and without 150 μM forskolin for 90 min, after which cells were fixed, permeabilized, and subjected to immunocytochemistry. AQP2 is shown in green, and the basolateral marker Na+/K+-ATPase is shown in red, alongside the nuclear stain DAPI. The z-profile images were reconstructed from multiple z-sections, along the dotted line. Mutant AQP2 fails to localize to the cell surface upon forskolin stimulation. Rather, the perinuclear staining is consistent with an ER localization of mutant AQP2. (C) The MDCK cell line expressing AQP2-F204V was grown on fibronectin-coated coverslips until tight junctions formed, at which point the cells were treated with 150 μM forskolin for 90 min. Cells were fixed, permeabilized, and sequentially immunoblotted for AQP2 (green) and calnexin (red), an ER marker. The merged image shows that AQP2-F204V colocalizes with the endoplasmic reticulum marker. Scale bar refers to 10 μm. To investigate the mechanism of defective translocation of AQP2-F204V, we turned to transfection of MDCK cells.
T10364 18028-18030 TO denotes To
T10365 18031-18042 VB denotes investigate
T10366 18102-18108 VBD denotes turned
T10367 18043-18046 DT denotes the
T10368 18047-18056 NN denotes mechanism
T10369 18057-18059 IN denotes of
T10370 18060-18069 JJ denotes defective
T10371 18070-18083 NN denotes translocation
T10372 18084-18086 IN denotes of
T10373 18087-18091 NN denotes AQP2
T10374 18092-18097 NN denotes F204V
T10375 18091-18092 HYPH denotes -
T10376 18097-18099 , denotes ,
T10377 18099-18101 PRP denotes we
T10378 18109-18111 IN denotes to
T10379 18112-18124 NN denotes transfection
T10380 18125-18127 IN denotes of
T10381 18128-18132 NN denotes MDCK
T10382 18133-18138 NNS denotes cells
T10383 18138-18139 . denotes .
T10384 18139-18222 sentence denotes Stable cell lines expressing mouse wild-type AQP2 and AQP2-F204V were established.
T10385 18140-18146 JJ denotes Stable
T10386 18152-18157 NNS denotes lines
T10387 18147-18151 NN denotes cell
T10388 18210-18221 VBN denotes established
T10389 18158-18168 VBG denotes expressing
T10390 18169-18174 NN denotes mouse
T10391 18185-18189 NN denotes AQP2
T10392 18175-18179 JJ denotes wild
T10393 18180-18184 NN denotes type
T10394 18179-18180 HYPH denotes -
T10395 18190-18193 CC denotes and
T10396 18194-18198 NN denotes AQP2
T10397 18199-18204 NN denotes F204V
T10398 18198-18199 HYPH denotes -
T10399 18205-18209 VBD denotes were
T10400 18221-18222 . denotes .
T10401 18222-18378 sentence denotes Immunoblots of protein extracts from stable cell lines showed that MDCK cells recapitulate the glycosylation defect seen in mutant mice (unpublished data).
T10402 18223-18234 NNS denotes Immunoblots
T10403 18278-18284 VBD denotes showed
T10404 18235-18237 IN denotes of
T10405 18238-18245 NN denotes protein
T10406 18246-18254 NNS denotes extracts
T10407 18255-18259 IN denotes from
T10408 18260-18266 JJ denotes stable
T10409 18272-18277 NNS denotes lines
T10410 18267-18271 NN denotes cell
T10411 18285-18289 IN denotes that
T10412 18301-18313 VBP denotes recapitulate
T10413 18290-18294 NN denotes MDCK
T10414 18295-18300 NNS denotes cells
T10415 18314-18317 DT denotes the
T10416 18332-18338 NN denotes defect
T10417 18318-18331 NN denotes glycosylation
T10418 18339-18343 VBN denotes seen
T10419 18344-18346 IN denotes in
T10420 18347-18353 NN denotes mutant
T10421 18354-18358 NNS denotes mice
T10422 18359-18360 -LRB- denotes (
T10423 18372-18376 NNS denotes data
T10424 18360-18371 JJ denotes unpublished
T10425 18376-18377 -RRB- denotes )
T10426 18377-18378 . denotes .
T10427 18378-18444 sentence denotes The wild-type protein was again present in three different forms.
T10428 18379-18382 DT denotes The
T10429 18393-18400 NN denotes protein
T10430 18383-18387 JJ denotes wild
T10431 18388-18392 NN denotes type
T10432 18387-18388 HYPH denotes -
T10433 18401-18404 VBD denotes was
T10434 18405-18410 RB denotes again
T10435 18411-18418 JJ denotes present
T10436 18419-18421 IN denotes in
T10437 18422-18427 CD denotes three
T10438 18438-18443 NNS denotes forms
T10439 18428-18437 JJ denotes different
T10440 18443-18444 . denotes .
T10441 18444-18554 sentence denotes Cells expressing AQP2-F204V lacked the 35–45 kDa form and were enriched in the core-glycosylated 31 kDa form.
T10442 18445-18450 NNS denotes Cells
T10443 18473-18479 VBD denotes lacked
T10444 18451-18461 VBG denotes expressing
T10445 18462-18466 NN denotes AQP2
T10446 18467-18472 NN denotes F204V
T10447 18466-18467 HYPH denotes -
T10448 18480-18483 DT denotes the
T10449 18494-18498 NN denotes form
T10450 18484-18486 CD denotes 35
T10451 18487-18489 CD denotes 45
T10452 18486-18487 SYM denotes –
T10453 18490-18493 NN denotes kDa
T10454 18499-18502 CC denotes and
T10455 18503-18507 VBD denotes were
T10456 18508-18516 VBN denotes enriched
T10457 18517-18519 IN denotes in
T10458 18520-18523 DT denotes the
T10459 18549-18553 NN denotes form
T10460 18524-18528 NN denotes core
T10461 18529-18541 VBN denotes glycosylated
T10462 18528-18529 HYPH denotes -
T10463 18542-18544 CD denotes 31
T10464 18545-18548 NN denotes kDa
T10465 18553-18554 . denotes .
T10466 18554-18792 sentence denotes In transfected, unstimulated MDCK cells, wild-type AQP2 (stained green) appeared in a punctate pattern distributed throughout the subapical region (Figure 4B, left column photomicrographs), consistent with vesicular compartmentalization.
T10467 18555-18557 IN denotes In
T10468 18627-18635 VBD denotes appeared
T10469 18558-18569 VBN denotes transfected
T10470 18589-18594 NNS denotes cells
T10471 18569-18571 , denotes ,
T10472 18571-18583 JJ denotes unstimulated
T10473 18584-18588 NN denotes MDCK
T10474 18594-18596 , denotes ,
T10475 18596-18600 JJ denotes wild
T10476 18601-18605 NN denotes type
T10477 18600-18601 HYPH denotes -
T10478 18606-18610 NN denotes AQP2
T10479 18611-18612 -LRB- denotes (
T10480 18612-18619 VBN denotes stained
T10481 18620-18625 JJ denotes green
T10482 18625-18626 -RRB- denotes )
T10483 18636-18638 IN denotes in
T10484 18639-18640 DT denotes a
T10485 18650-18657 NN denotes pattern
T10486 18641-18649 NN denotes punctate
T10487 18658-18669 VBN denotes distributed
T10488 18670-18680 IN denotes throughout
T10489 18681-18684 DT denotes the
T10490 18695-18701 NN denotes region
T10491 18685-18694 JJ denotes subapical
T10492 18702-18703 -LRB- denotes (
T10493 18726-18742 NNS denotes photomicrographs
T10494 18703-18709 NN denotes Figure
T10495 18710-18712 CD denotes 4B
T10496 18712-18714 , denotes ,
T10497 18714-18718 JJ denotes left
T10498 18719-18725 NN denotes column
T10499 18742-18743 -RRB- denotes )
T10500 18743-18745 , denotes ,
T10501 18745-18755 JJ denotes consistent
T10502 18756-18760 IN denotes with
T10503 18761-18770 JJ denotes vesicular
T10504 18771-18791 NN denotes compartmentalization
T10505 18791-18792 . denotes .
T10506 18792-18897 sentence denotes AQP2-F204V, on the other hand, appeared in a punctate but perinuclear pattern (Figure 4B, third column).
T10507 18793-18797 NN denotes AQP2
T10508 18798-18803 NN denotes F204V
T10509 18797-18798 HYPH denotes -
T10510 18824-18832 VBD denotes appeared
T10511 18803-18805 , denotes ,
T10512 18805-18807 IN denotes on
T10513 18808-18811 DT denotes the
T10514 18818-18822 NN denotes hand
T10515 18812-18817 JJ denotes other
T10516 18822-18824 , denotes ,
T10517 18833-18835 IN denotes in
T10518 18836-18837 DT denotes a
T10519 18863-18870 NN denotes pattern
T10520 18838-18846 NN denotes punctate
T10521 18851-18862 JJ denotes perinuclear
T10522 18847-18850 CC denotes but
T10523 18871-18872 -LRB- denotes (
T10524 18889-18895 NN denotes column
T10525 18872-18878 NN denotes Figure
T10526 18879-18881 CD denotes 4B
T10527 18881-18883 , denotes ,
T10528 18883-18888 JJ denotes third
T10529 18895-18896 -RRB- denotes )
T10530 18896-18897 . denotes .
T10531 18897-19075 sentence denotes Upon stimulation with forskolin, a cAMP-dependent protein kinase activator, wild-type AQP2 translocated to the apical surface of polarized MDCK cells (Figure 4B, second column).
T10532 18898-18902 IN denotes Upon
T10533 18989-19001 VBD denotes translocated
T10534 18903-18914 NN denotes stimulation
T10535 18915-18919 IN denotes with
T10536 18920-18929 NN denotes forskolin
T10537 18929-18931 , denotes ,
T10538 18931-18932 DT denotes a
T10539 18963-18972 NN denotes activator
T10540 18933-18937 NN denotes cAMP
T10541 18938-18947 JJ denotes dependent
T10542 18937-18938 HYPH denotes -
T10543 18948-18955 NN denotes protein
T10544 18956-18962 NN denotes kinase
T10545 18972-18974 , denotes ,
T10546 18974-18978 JJ denotes wild
T10547 18979-18983 NN denotes type
T10548 18978-18979 HYPH denotes -
T10549 18984-18988 NN denotes AQP2
T10550 19002-19004 IN denotes to
T10551 19005-19008 DT denotes the
T10552 19016-19023 NN denotes surface
T10553 19009-19015 JJ denotes apical
T10554 19024-19026 IN denotes of
T10555 19027-19036 VBN denotes polarized
T10556 19042-19047 NNS denotes cells
T10557 19037-19041 NN denotes MDCK
T10558 19048-19049 -LRB- denotes (
T10559 19067-19073 NN denotes column
T10560 19049-19055 NN denotes Figure
T10561 19056-19058 CD denotes 4B
T10562 19058-19060 , denotes ,
T10563 19060-19066 JJ denotes second
T10564 19073-19074 -RRB- denotes )
T10565 19074-19075 . denotes .
T10566 19075-19248 sentence denotes Along the z-axis, the perinuclear distribution of AQP2-F204V was clearly seen, and this distribution is not altered by forskolin (Figure 4B, bottom row, two right columns).
T10567 19076-19081 IN denotes Along
T10568 19149-19153 VBN denotes seen
T10569 19082-19085 DT denotes the
T10570 19088-19092 NN denotes axis
T10571 19086-19087 NN denotes z
T10572 19087-19088 HYPH denotes -
T10573 19092-19094 , denotes ,
T10574 19094-19097 DT denotes the
T10575 19110-19122 NN denotes distribution
T10576 19098-19109 JJ denotes perinuclear
T10577 19123-19125 IN denotes of
T10578 19126-19130 NN denotes AQP2
T10579 19131-19136 NN denotes F204V
T10580 19130-19131 HYPH denotes -
T10581 19137-19140 VBD denotes was
T10582 19141-19148 RB denotes clearly
T10583 19153-19155 , denotes ,
T10584 19155-19158 CC denotes and
T10585 19159-19163 DT denotes this
T10586 19164-19176 NN denotes distribution
T10587 19184-19191 VBN denotes altered
T10588 19177-19179 VBZ denotes is
T10589 19180-19183 RB denotes not
T10590 19192-19194 IN denotes by
T10591 19195-19204 NN denotes forskolin
T10592 19205-19206 -LRB- denotes (
T10593 19239-19246 NNS denotes columns
T10594 19206-19212 NN denotes Figure
T10595 19213-19215 CD denotes 4B
T10596 19215-19217 , denotes ,
T10597 19217-19223 JJ denotes bottom
T10598 19224-19227 NN denotes row
T10599 19227-19229 , denotes ,
T10600 19229-19232 CD denotes two
T10601 19233-19238 JJ denotes right
T10602 19246-19247 -RRB- denotes )
T10603 19247-19248 . denotes .
T10604 19248-19338 sentence denotes The perinuclear distribution of AQP2-F204V is consistent with an ER compartmentalization.
T10605 19249-19252 DT denotes The
T10606 19265-19277 NN denotes distribution
T10607 19253-19264 JJ denotes perinuclear
T10608 19292-19294 VBZ denotes is
T10609 19278-19280 IN denotes of
T10610 19281-19285 NN denotes AQP2
T10611 19286-19291 NN denotes F204V
T10612 19285-19286 HYPH denotes -
T10613 19295-19305 JJ denotes consistent
T10614 19306-19310 IN denotes with
T10615 19311-19313 DT denotes an
T10616 19317-19337 NN denotes compartmentalization
T10617 19314-19316 NN denotes ER
T10618 19337-19338 . denotes .
T10619 19338-19495 sentence denotes To test the idea that AQP2-F204V localizes to the ER, we co-stained cells transfected with Aqp2F204V (cDNA) for AQP2 and an ER marker, calnexin (Figure 4C).
T10620 19339-19341 TO denotes To
T10621 19342-19346 VB denotes test
T10622 19396-19406 VBD denotes co-stained
T10623 19347-19350 DT denotes the
T10624 19351-19355 NN denotes idea
T10625 19356-19360 IN denotes that
T10626 19372-19381 VBZ denotes localizes
T10627 19361-19365 NN denotes AQP2
T10628 19366-19371 NN denotes F204V
T10629 19365-19366 HYPH denotes -
T10630 19382-19384 IN denotes to
T10631 19385-19388 DT denotes the
T10632 19389-19391 NN denotes ER
T10633 19391-19393 , denotes ,
T10634 19393-19395 PRP denotes we
T10635 19407-19412 NNS denotes cells
T10636 19413-19424 VBN denotes transfected
T10637 19425-19429 IN denotes with
T10638 19430-19439 NN denotes Aqp2F204V
T10639 19440-19441 -LRB- denotes (
T10640 19441-19445 NN denotes cDNA
T10641 19445-19446 -RRB- denotes )
T10642 19447-19450 IN denotes for
T10643 19451-19455 NN denotes AQP2
T10644 19456-19459 CC denotes and
T10645 19460-19462 DT denotes an
T10646 19466-19472 NN denotes marker
T10647 19463-19465 NN denotes ER
T10648 19472-19474 , denotes ,
T10649 19474-19482 NN denotes calnexin
T10650 19483-19484 -LRB- denotes (
T10651 19484-19490 NN denotes Figure
T10652 19491-19493 CD denotes 4C
T10653 19493-19494 -RRB- denotes )
T10654 19494-19495 . denotes .
T10655 19495-19638 sentence denotes Colocalization of calnexin with AQP2 was investigated directly, and it was found that 80% of all AQP2-F204V protein colocalized with calnexin.
T10656 19496-19510 NN denotes Colocalization
T10657 19537-19549 VBN denotes investigated
T10658 19511-19513 IN denotes of
T10659 19514-19522 NN denotes calnexin
T10660 19523-19527 IN denotes with
T10661 19528-19532 NN denotes AQP2
T10662 19533-19536 VBD denotes was
T10663 19550-19558 RB denotes directly
T10664 19558-19560 , denotes ,
T10665 19560-19563 CC denotes and
T10666 19564-19566 PRP denotes it
T10667 19571-19576 VBN denotes found
T10668 19567-19570 VBD denotes was
T10669 19577-19581 IN denotes that
T10670 19612-19623 VBD denotes colocalized
T10671 19582-19584 CD denotes 80
T10672 19584-19585 NN denotes %
T10673 19586-19588 IN denotes of
T10674 19589-19592 DT denotes all
T10675 19604-19611 NN denotes protein
T10676 19593-19597 NN denotes AQP2
T10677 19598-19603 NN denotes F204V
T10678 19597-19598 HYPH denotes -
T10679 19624-19628 IN denotes with
T10680 19629-19637 NN denotes calnexin
T10681 19637-19638 . denotes .
T10682 19638-19764 sentence denotes The remaining 20% appeared at the periphery of the ER, representing AQP2-F204V that had potentially progressed beyond the ER.
T10683 19639-19642 DT denotes The
T10684 19655-19656 NN denotes %
T10685 19643-19652 VBG denotes remaining
T10686 19653-19655 CD denotes 20
T10687 19657-19665 VBD denotes appeared
T10688 19666-19668 IN denotes at
T10689 19669-19672 DT denotes the
T10690 19673-19682 NN denotes periphery
T10691 19683-19685 IN denotes of
T10692 19686-19689 DT denotes the
T10693 19690-19692 NN denotes ER
T10694 19692-19694 , denotes ,
T10695 19694-19706 VBG denotes representing
T10696 19707-19711 NN denotes AQP2
T10697 19712-19717 NN denotes F204V
T10698 19711-19712 HYPH denotes -
T10699 19718-19722 WDT denotes that
T10700 19739-19749 VBN denotes progressed
T10701 19723-19726 VBD denotes had
T10702 19727-19738 RB denotes potentially
T10703 19750-19756 IN denotes beyond
T10704 19757-19760 DT denotes the
T10705 19761-19763 NN denotes ER
T10706 19763-19764 . denotes .
T10707 19764-19901 sentence denotes This “ER escape” was consistent with the small proportion of mature, complex glycosylated, AQP2-F204V in mutant kidneys (see Figure 3A).
T10708 19765-19769 DT denotes This
T10709 19774-19780 NN denotes escape
T10710 19770-19771 `` denotes “
T10711 19771-19773 NN denotes ER
T10712 19782-19785 VBD denotes was
T10713 19780-19781 '' denotes ”
T10714 19786-19796 JJ denotes consistent
T10715 19797-19801 IN denotes with
T10716 19802-19805 DT denotes the
T10717 19812-19822 NN denotes proportion
T10718 19806-19811 JJ denotes small
T10719 19823-19825 IN denotes of
T10720 19826-19832 JJ denotes mature
T10721 19861-19866 NN denotes F204V
T10722 19832-19834 , denotes ,
T10723 19834-19841 JJ denotes complex
T10724 19842-19854 VBN denotes glycosylated
T10725 19854-19856 , denotes ,
T10726 19856-19860 NN denotes AQP2
T10727 19860-19861 HYPH denotes -
T10728 19867-19869 IN denotes in
T10729 19870-19876 NN denotes mutant
T10730 19877-19884 NNS denotes kidneys
T10731 19885-19886 -LRB- denotes (
T10732 19886-19889 VB denotes see
T10733 19890-19896 NN denotes Figure
T10734 19897-19899 CD denotes 3A
T10735 19899-19900 -RRB- denotes )
T10736 19900-19901 . denotes .
T10737 19901-20037 sentence denotes Animals heterozygous for the Aqp2F204V mutation were not affected in their urine production or urine osmolality (see Figure 1C and 1D).
T10738 19902-19909 NNS denotes Animals
T10739 19959-19967 VBN denotes affected
T10740 19910-19922 JJ denotes heterozygous
T10741 19923-19926 IN denotes for
T10742 19927-19930 DT denotes the
T10743 19941-19949 NN denotes mutation
T10744 19931-19940 NN denotes Aqp2F204V
T10745 19950-19954 VBD denotes were
T10746 19955-19958 RB denotes not
T10747 19968-19970 IN denotes in
T10748 19971-19976 PRP$ denotes their
T10749 19983-19993 NN denotes production
T10750 19977-19982 NN denotes urine
T10751 19994-19996 CC denotes or
T10752 19997-20002 NN denotes urine
T10753 20003-20013 NN denotes osmolality
T10754 20014-20015 -LRB- denotes (
T10755 20015-20018 VB denotes see
T10756 20019-20025 NN denotes Figure
T10757 20026-20028 CD denotes 1C
T10758 20029-20032 CC denotes and
T10759 20033-20035 CD denotes 1D
T10760 20035-20036 -RRB- denotes )
T10761 20036-20037 . denotes .
T10762 20037-20208 sentence denotes It has also been shown that a recessive NDI allele, AQP2-R187C, does not interact with wild-type protein in oocytes [24], nor does it homo-oligomerize in MDCK cells [22].
T10763 20038-20040 PRP denotes It
T10764 20055-20060 VBN denotes shown
T10765 20041-20044 VBZ denotes has
T10766 20045-20049 RB denotes also
T10767 20050-20054 VBN denotes been
T10768 20061-20065 IN denotes that
T10769 20111-20119 VB denotes interact
T10770 20066-20067 DT denotes a
T10771 20082-20088 NN denotes allele
T10772 20068-20077 JJ denotes recessive
T10773 20078-20081 NN denotes NDI
T10774 20088-20090 , denotes ,
T10775 20090-20094 NN denotes AQP2
T10776 20095-20100 NN denotes R187C
T10777 20094-20095 HYPH denotes -
T10778 20100-20102 , denotes ,
T10779 20102-20106 VBZ denotes does
T10780 20107-20110 RB denotes not
T10781 20120-20124 IN denotes with
T10782 20125-20129 JJ denotes wild
T10783 20130-20134 NN denotes type
T10784 20129-20130 HYPH denotes -
T10785 20135-20142 NN denotes protein
T10786 20143-20145 IN denotes in
T10787 20146-20153 NNS denotes oocytes
T10788 20154-20155 -LRB- denotes [
T10789 20155-20157 CD denotes 24
T10790 20157-20158 -RRB- denotes ]
T10791 20158-20160 , denotes ,
T10792 20160-20163 CC denotes nor
T10793 20164-20168 VBZ denotes does
T10794 20172-20188 VB denotes homo-oligomerize
T10795 20169-20171 PRP denotes it
T10796 20189-20191 IN denotes in
T10797 20192-20196 NN denotes MDCK
T10798 20197-20202 NNS denotes cells
T10799 20203-20204 -LRB- denotes [
T10800 20204-20206 CD denotes 22
T10801 20206-20207 -RRB- denotes ]
T10802 20207-20208 . denotes .
T10803 20208-20316 sentence denotes Therefore, kidneys from heterozygous animals were examined for evidence of two populations of AQP2 protein.
T10804 20209-20218 RB denotes Therefore
T10805 20259-20267 VBN denotes examined
T10806 20218-20220 , denotes ,
T10807 20220-20227 NNS denotes kidneys
T10808 20228-20232 IN denotes from
T10809 20233-20245 JJ denotes heterozygous
T10810 20246-20253 NNS denotes animals
T10811 20254-20258 VBD denotes were
T10812 20268-20271 IN denotes for
T10813 20272-20280 NN denotes evidence
T10814 20281-20283 IN denotes of
T10815 20284-20287 CD denotes two
T10816 20288-20299 NNS denotes populations
T10817 20300-20302 IN denotes of
T10818 20303-20307 NN denotes AQP2
T10819 20308-20315 NN denotes protein
T10820 20315-20316 . denotes .
T10821 20316-20472 sentence denotes Surprisingly, immunohistochemical staining of kidney collecting ducts from Aqp2F204V/+ mice revealed a pattern remarkably similar to wild type (Figure 5A).
T10822 20317-20329 RB denotes Surprisingly
T10823 20409-20417 VBD denotes revealed
T10824 20329-20331 , denotes ,
T10825 20331-20350 JJ denotes immunohistochemical
T10826 20351-20359 NN denotes staining
T10827 20360-20362 IN denotes of
T10828 20363-20369 NN denotes kidney
T10829 20381-20386 NNS denotes ducts
T10830 20370-20380 VBG denotes collecting
T10831 20387-20391 IN denotes from
T10832 20392-20401 NN denotes Aqp2F204V
T10833 20404-20408 NNS denotes mice
T10834 20401-20402 HYPH denotes /
T10835 20402-20403 SYM denotes +
T10836 20418-20419 DT denotes a
T10837 20420-20427 NN denotes pattern
T10838 20428-20438 RB denotes remarkably
T10839 20439-20446 JJ denotes similar
T10840 20447-20449 IN denotes to
T10841 20450-20454 JJ denotes wild
T10842 20455-20459 NN denotes type
T10843 20460-20461 -LRB- denotes (
T10844 20461-20467 NN denotes Figure
T10845 20468-20470 CD denotes 5A
T10846 20470-20471 -RRB- denotes )
T10847 20471-20472 . denotes .
T10848 20472-20552 sentence denotes AQP2 translocated completely to the apical cell surface upon dDAVP stimulation.
T10849 20473-20477 NN denotes AQP2
T10850 20478-20490 VBD denotes translocated
T10851 20491-20501 RB denotes completely
T10852 20502-20504 IN denotes to
T10853 20505-20508 DT denotes the
T10854 20521-20528 NN denotes surface
T10855 20509-20515 JJ denotes apical
T10856 20516-20520 NN denotes cell
T10857 20529-20533 IN denotes upon
T10858 20534-20539 NN denotes dDAVP
T10859 20540-20551 NN denotes stimulation
T10860 20551-20552 . denotes .
T10861 20552-20711 sentence denotes This wild-type staining pattern may simply reflect the fact that decreasing the amount of mutant protein by half makes it undetectable by immunocytochemistry.
T10862 20553-20557 DT denotes This
T10863 20577-20584 NN denotes pattern
T10864 20558-20562 JJ denotes wild
T10865 20563-20567 NN denotes type
T10866 20562-20563 HYPH denotes -
T10867 20568-20576 NN denotes staining
T10868 20596-20603 VB denotes reflect
T10869 20585-20588 MD denotes may
T10870 20589-20595 RB denotes simply
T10871 20604-20607 DT denotes the
T10872 20608-20612 NN denotes fact
T10873 20613-20617 IN denotes that
T10874 20666-20671 VBZ denotes makes
T10875 20618-20628 VBG denotes decreasing
T10876 20629-20632 DT denotes the
T10877 20633-20639 NN denotes amount
T10878 20640-20642 IN denotes of
T10879 20643-20649 NN denotes mutant
T10880 20650-20657 NN denotes protein
T10881 20658-20660 IN denotes by
T10882 20661-20665 NN denotes half
T10883 20672-20674 PRP denotes it
T10884 20675-20687 JJ denotes undetectable
T10885 20688-20690 IN denotes by
T10886 20691-20710 NN denotes immunocytochemistry
T10887 20710-20711 . denotes .
T10888 20711-20810 sentence denotes Alternatively, the presence of wild-type protein may alter the localization of the mutant protein.
T10889 20712-20725 RB denotes Alternatively
T10890 20765-20770 VB denotes alter
T10891 20725-20727 , denotes ,
T10892 20727-20730 DT denotes the
T10893 20731-20739 NN denotes presence
T10894 20740-20742 IN denotes of
T10895 20743-20747 JJ denotes wild
T10896 20748-20752 NN denotes type
T10897 20747-20748 HYPH denotes -
T10898 20753-20760 NN denotes protein
T10899 20761-20764 MD denotes may
T10900 20771-20774 DT denotes the
T10901 20775-20787 NN denotes localization
T10902 20788-20790 IN denotes of
T10903 20791-20794 DT denotes the
T10904 20802-20809 NN denotes protein
T10905 20795-20801 NN denotes mutant
T10906 20809-20810 . denotes .
T10907 20810-20980 sentence denotes Indeed, Hendriks et al. proposed a “piggy-back” mechanism to explain the transport of nonglycosylated subunits of AQP2 to the cell surface by glycosylated subunits [22].
T10908 20811-20817 RB denotes Indeed
T10909 20835-20843 VBD denotes proposed
T10910 20817-20819 , denotes ,
T10911 20819-20827 NNP denotes Hendriks
T10912 20828-20830 FW denotes et
T10913 20831-20834 FW denotes al.
T10914 20844-20845 DT denotes a
T10915 20859-20868 NN denotes mechanism
T10916 20846-20847 `` denotes “
T10917 20847-20852 NN denotes piggy
T10918 20853-20857 NN denotes back
T10919 20852-20853 HYPH denotes -
T10920 20857-20858 '' denotes ”
T10921 20869-20871 TO denotes to
T10922 20872-20879 VB denotes explain
T10923 20880-20883 DT denotes the
T10924 20884-20893 NN denotes transport
T10925 20894-20896 IN denotes of
T10926 20897-20912 JJ denotes nonglycosylated
T10927 20913-20921 NNS denotes subunits
T10928 20922-20924 IN denotes of
T10929 20925-20929 NN denotes AQP2
T10930 20930-20932 IN denotes to
T10931 20933-20936 DT denotes the
T10932 20942-20949 NN denotes surface
T10933 20937-20941 NN denotes cell
T10934 20950-20952 IN denotes by
T10935 20953-20965 VBN denotes glycosylated
T10936 20966-20974 NNS denotes subunits
T10937 20975-20976 -LRB- denotes [
T10938 20976-20978 CD denotes 22
T10939 20978-20979 -RRB- denotes ]
T10940 20979-20980 . denotes .
T10941 20980-21145 sentence denotes It has also been shown that wild-type AQP2 protein can rescue a translocation-defective mutant protein, AQP2-P262L, when the two are coexpressed in MDCK cells [25].
T10942 20981-20983 PRP denotes It
T10943 20998-21003 VBN denotes shown
T10944 20984-20987 VBZ denotes has
T10945 20988-20992 RB denotes also
T10946 20993-20997 VBN denotes been
T10947 21004-21008 IN denotes that
T10948 21036-21042 VB denotes rescue
T10949 21009-21013 JJ denotes wild
T10950 21014-21018 NN denotes type
T10951 21013-21014 HYPH denotes -
T10952 21024-21031 NN denotes protein
T10953 21019-21023 NN denotes AQP2
T10954 21032-21035 MD denotes can
T10955 21043-21044 DT denotes a
T10956 21076-21083 NN denotes protein
T10957 21045-21058 NN denotes translocation
T10958 21059-21068 JJ denotes defective
T10959 21058-21059 HYPH denotes -
T10960 21069-21075 NN denotes mutant
T10961 21083-21085 , denotes ,
T10962 21085-21089 NN denotes AQP2
T10963 21090-21095 NN denotes P262L
T10964 21089-21090 HYPH denotes -
T10965 21095-21097 , denotes ,
T10966 21097-21101 WRB denotes when
T10967 21114-21125 VBN denotes coexpressed
T10968 21102-21105 DT denotes the
T10969 21106-21109 CD denotes two
T10970 21110-21113 VBP denotes are
T10971 21126-21128 IN denotes in
T10972 21129-21133 NN denotes MDCK
T10973 21134-21139 NNS denotes cells
T10974 21140-21141 -LRB- denotes [
T10975 21141-21143 CD denotes 25
T10976 21143-21144 -RRB- denotes ]
T10977 21144-21145 . denotes .
T10978 21145-22908 sentence denotes Figure 5 AQP2-F204V Rescue in Heterozygous Mouse Collecting Ducts and in Cotransfected MDCK Cells (A) In heterozygous animals, AQP2 localizes and responds to dDAVP normally. Immunohistochemistry was carried out on kidney sections from an Aqp2F204V/+ mouse, after injection with dDAVP. Kidney sections were sequentially immunostained for AQP2 (red) and the basolateral marker AQP3 (green). (B) Mutant and wild-type AQP2 physically interact. MDCK cells stably expressing wild-type AQP2 were transiently transfected with GFP tagged wild-type AQP2, AQP2-F204V, or GFP alone. Solubilized membranes were immunoprecipitated with a GFP antibody. Total membranes and immunoprecipitates (GFP-IP) were Western blotted using an antibody against AQP2 (arrow) or AQP2-GFP fusions (arrowhead). (C) Wild-type AQP2 rescues the localization defect of mutant AQP2. GFP fusions of either wild-type AQP2 (WT-GFP, top photomicrographs) or F204V AQP2 (F204V-GFP, bottom photomicrographs) were expressed in polarized MDCK stable cell lines expressing vector alone (vector, left photomicrographs) or AQP2-WT (right photomicrographs). Cells were stimulated with forskolin, processed for immunocytochemistry, and used to generate z-sectional images. (D) Mutant AQP2 is present at the cell surface in cells coexpressing wild-type AQP2 (AQP2-WT). GFP fused to AQP2-F204V was expressed in MDCK cells expressing wild-type AQP2 or vector alone. Cells were stimulated with forskolin, and cell surface biotinylated proteins were precipitated then analyzed for the presence of wild-type AQP2 (arrow) and AQP2-F204V (arrowhead) by Western blot. In the collecting ducts from Aqp2F204V/+ mice, the wild type may rescue the mutant protein as suggested by the subcellular distribution of AQP2 protein.
T10979 22756-22758 IN denotes In
T10980 22821-22827 VB denotes rescue
T10981 22759-22762 DT denotes the
T10982 22774-22779 NNS denotes ducts
T10983 22763-22773 VBG denotes collecting
T10984 22780-22784 IN denotes from
T10985 22785-22794 NN denotes Aqp2F204V
T10986 22797-22801 NNS denotes mice
T10987 22794-22795 HYPH denotes /
T10988 22795-22796 SYM denotes +
T10989 22801-22803 , denotes ,
T10990 22803-22806 DT denotes the
T10991 22812-22816 NN denotes type
T10992 22807-22811 JJ denotes wild
T10993 22817-22820 MD denotes may
T10994 22828-22831 DT denotes the
T10995 22839-22846 NN denotes protein
T10996 22832-22838 NN denotes mutant
T10997 22847-22849 IN denotes as
T10998 22850-22859 VBN denotes suggested
T10999 22860-22862 IN denotes by
T11000 22863-22866 DT denotes the
T11001 22879-22891 NN denotes distribution
T11002 22867-22878 JJ denotes subcellular
T11003 22892-22894 IN denotes of
T11004 22895-22899 NN denotes AQP2
T11005 22900-22907 NN denotes protein
T11006 22907-22908 . denotes .
T11007 22908-23039 sentence denotes To test this idea, we first looked for an interaction between mutant and wild-type proteins in transfected MDCK cells (Figure 5B).
T11008 22909-22911 TO denotes To
T11009 22912-22916 VB denotes test
T11010 22937-22943 VBD denotes looked
T11011 22917-22921 DT denotes this
T11012 22922-22926 NN denotes idea
T11013 22926-22928 , denotes ,
T11014 22928-22930 PRP denotes we
T11015 22931-22936 RB denotes first
T11016 22944-22947 IN denotes for
T11017 22948-22950 DT denotes an
T11018 22951-22962 NN denotes interaction
T11019 22963-22970 IN denotes between
T11020 22971-22977 NN denotes mutant
T11021 22992-23000 NN denotes proteins
T11022 22978-22981 CC denotes and
T11023 22982-22986 JJ denotes wild
T11024 22987-22991 NN denotes type
T11025 22986-22987 HYPH denotes -
T11026 23001-23003 IN denotes in
T11027 23004-23015 VBN denotes transfected
T11028 23021-23026 NNS denotes cells
T11029 23016-23020 NN denotes MDCK
T11030 23027-23028 -LRB- denotes (
T11031 23028-23034 NN denotes Figure
T11032 23035-23037 CD denotes 5B
T11033 23037-23038 -RRB- denotes )
T11034 23038-23039 . denotes .
T11035 23039-23205 sentence denotes MDCK cells stably expressing wild-type AQP2 were transiently transfected with GFP expression constructs encoding GFP-tagged wild-type AQP2, AQP2-F204V, or GFP alone.
T11036 23040-23044 NN denotes MDCK
T11037 23045-23050 NNS denotes cells
T11038 23101-23112 VBN denotes transfected
T11039 23051-23057 RB denotes stably
T11040 23058-23068 VBG denotes expressing
T11041 23069-23073 JJ denotes wild
T11042 23074-23078 NN denotes type
T11043 23073-23074 HYPH denotes -
T11044 23079-23083 NN denotes AQP2
T11045 23084-23088 VBD denotes were
T11046 23089-23100 RB denotes transiently
T11047 23113-23117 IN denotes with
T11048 23118-23121 NN denotes GFP
T11049 23133-23143 NNS denotes constructs
T11050 23122-23132 NN denotes expression
T11051 23144-23152 VBG denotes encoding
T11052 23153-23156 NN denotes GFP
T11053 23157-23163 VBN denotes tagged
T11054 23156-23157 HYPH denotes -
T11055 23174-23178 NN denotes AQP2
T11056 23164-23168 JJ denotes wild
T11057 23169-23173 NN denotes type
T11058 23168-23169 HYPH denotes -
T11059 23178-23180 , denotes ,
T11060 23180-23184 NN denotes AQP2
T11061 23185-23190 NN denotes F204V
T11062 23184-23185 HYPH denotes -
T11063 23190-23192 , denotes ,
T11064 23192-23194 CC denotes or
T11065 23195-23198 NN denotes GFP
T11066 23199-23204 RB denotes alone
T11067 23204-23205 . denotes .
T11068 23205-23455 sentence denotes Antibodies against GFP coimmunoprecipitated wild-type AQP2 when AQP2-GFP or AQP2-F204V-GFP was transiently transfected, but not when GFP by itself was transiently transfected into MDCK cells stably expressing wild-type AQP2 (Figure 5B, upper blots).
T11069 23206-23216 NNS denotes Antibodies
T11070 23229-23249 VBD denotes coimmunoprecipitated
T11071 23217-23224 IN denotes against
T11072 23225-23228 NN denotes GFP
T11073 23250-23254 JJ denotes wild
T11074 23255-23259 NN denotes type
T11075 23254-23255 HYPH denotes -
T11076 23260-23264 NN denotes AQP2
T11077 23265-23269 WRB denotes when
T11078 23313-23324 VBN denotes transfected
T11079 23270-23274 NN denotes AQP2
T11080 23275-23278 NN denotes GFP
T11081 23274-23275 HYPH denotes -
T11082 23279-23281 CC denotes or
T11083 23282-23286 NN denotes AQP2
T11084 23293-23296 NN denotes GFP
T11085 23286-23287 HYPH denotes -
T11086 23287-23292 NN denotes F204V
T11087 23292-23293 HYPH denotes -
T11088 23297-23300 VBD denotes was
T11089 23301-23312 RB denotes transiently
T11090 23324-23326 , denotes ,
T11091 23326-23329 CC denotes but
T11092 23330-23333 RB denotes not
T11093 23334-23338 WRB denotes when
T11094 23369-23380 VBN denotes transfected
T11095 23339-23342 NN denotes GFP
T11096 23343-23345 IN denotes by
T11097 23346-23352 PRP denotes itself
T11098 23353-23356 VBD denotes was
T11099 23357-23368 RB denotes transiently
T11100 23381-23385 IN denotes into
T11101 23386-23390 NN denotes MDCK
T11102 23391-23396 NNS denotes cells
T11103 23397-23403 RB denotes stably
T11104 23404-23414 VBG denotes expressing
T11105 23415-23419 JJ denotes wild
T11106 23420-23424 NN denotes type
T11107 23419-23420 HYPH denotes -
T11108 23425-23429 NN denotes AQP2
T11109 23430-23431 -LRB- denotes (
T11110 23448-23453 NNS denotes blots
T11111 23431-23437 NN denotes Figure
T11112 23438-23440 CD denotes 5B
T11113 23440-23442 , denotes ,
T11114 23442-23447 JJ denotes upper
T11115 23453-23454 -RRB- denotes )
T11116 23454-23455 . denotes .
T11117 23455-23585 sentence denotes Western blot of total membranes showed that wild-type AQP2 is equivalently expressed in all three cases (Figure 5B, lower blots).
T11118 23456-23463 NNP denotes Western
T11119 23464-23468 NN denotes blot
T11120 23488-23494 VBD denotes showed
T11121 23469-23471 IN denotes of
T11122 23472-23477 JJ denotes total
T11123 23478-23487 NNS denotes membranes
T11124 23495-23499 IN denotes that
T11125 23531-23540 VBN denotes expressed
T11126 23500-23504 JJ denotes wild
T11127 23505-23509 NN denotes type
T11128 23504-23505 HYPH denotes -
T11129 23510-23514 NN denotes AQP2
T11130 23515-23517 VBZ denotes is
T11131 23518-23530 RB denotes equivalently
T11132 23541-23543 IN denotes in
T11133 23544-23547 DT denotes all
T11134 23554-23559 NNS denotes cases
T11135 23548-23553 CD denotes three
T11136 23560-23561 -LRB- denotes (
T11137 23578-23583 NNS denotes blots
T11138 23561-23567 NN denotes Figure
T11139 23568-23570 CD denotes 5B
T11140 23570-23572 , denotes ,
T11141 23572-23577 JJ denotes lower
T11142 23583-23584 -RRB- denotes )
T11143 23584-23585 . denotes .
T11144 23585-23731 sentence denotes If wild-type and mutant proteins are indeed interacting in the cell, is this interaction sufficient to rescue the localization of mutant protein?
T11145 23586-23588 IN denotes If
T11146 23630-23641 VBG denotes interacting
T11147 23589-23593 JJ denotes wild
T11148 23594-23598 NN denotes type
T11149 23593-23594 HYPH denotes -
T11150 23610-23618 NN denotes proteins
T11151 23599-23602 CC denotes and
T11152 23603-23609 NN denotes mutant
T11153 23619-23622 VBP denotes are
T11154 23623-23629 RB denotes indeed
T11155 23655-23657 VBZ denotes is
T11156 23642-23644 IN denotes in
T11157 23645-23648 DT denotes the
T11158 23649-23653 NN denotes cell
T11159 23653-23655 , denotes ,
T11160 23658-23662 DT denotes this
T11161 23663-23674 NN denotes interaction
T11162 23675-23685 JJ denotes sufficient
T11163 23686-23688 TO denotes to
T11164 23689-23695 VB denotes rescue
T11165 23696-23699 DT denotes the
T11166 23700-23712 NN denotes localization
T11167 23713-23715 IN denotes of
T11168 23716-23722 NN denotes mutant
T11169 23723-23730 NN denotes protein
T11170 23730-23731 . denotes ?
T11171 23731-23854 sentence denotes To answer this question, we used MDCK cells stably transfected with wild-type AQP2 expressing vector or with empty vector.
T11172 23732-23734 TO denotes To
T11173 23735-23741 VB denotes answer
T11174 23760-23764 VBD denotes used
T11175 23742-23746 DT denotes this
T11176 23747-23755 NN denotes question
T11177 23755-23757 , denotes ,
T11178 23757-23759 PRP denotes we
T11179 23765-23769 NN denotes MDCK
T11180 23770-23775 NNS denotes cells
T11181 23776-23782 RB denotes stably
T11182 23783-23794 VBN denotes transfected
T11183 23795-23799 IN denotes with
T11184 23800-23804 JJ denotes wild
T11185 23805-23809 NN denotes type
T11186 23804-23805 HYPH denotes -
T11187 23810-23814 NN denotes AQP2
T11188 23815-23825 VBG denotes expressing
T11189 23826-23832 NN denotes vector
T11190 23833-23835 CC denotes or
T11191 23836-23840 IN denotes with
T11192 23841-23846 JJ denotes empty
T11193 23847-23853 NN denotes vector
T11194 23853-23854 . denotes .
T11195 23854-23948 sentence denotes On top of these, we transiently transfected AQP2-GFP or AQP2-F204V-GFP expression constructs.
T11196 23855-23857 IN denotes On
T11197 23887-23898 VBD denotes transfected
T11198 23858-23861 NN denotes top
T11199 23862-23864 IN denotes of
T11200 23865-23870 DT denotes these
T11201 23870-23872 , denotes ,
T11202 23872-23874 PRP denotes we
T11203 23875-23886 RB denotes transiently
T11204 23899-23903 NN denotes AQP2
T11205 23904-23907 NN denotes GFP
T11206 23903-23904 HYPH denotes -
T11207 23937-23947 NNS denotes constructs
T11208 23908-23910 CC denotes or
T11209 23911-23915 NN denotes AQP2
T11210 23922-23925 NN denotes GFP
T11211 23915-23916 HYPH denotes -
T11212 23916-23921 NN denotes F204V
T11213 23921-23922 HYPH denotes -
T11214 23926-23936 NN denotes expression
T11215 23947-23948 . denotes .
T11216 23948-24164 sentence denotes AQP2-GFP localized to the apical surface upon forskolin stimulation whether it was transiently transfected into vector-only cells (Figure 5C, upper left images) or into wild-type AQP2 cells (Figure 5C, upper right).
T11217 23949-23953 NN denotes AQP2
T11218 23954-23957 NN denotes GFP
T11219 23953-23954 HYPH denotes -
T11220 23958-23967 VBD denotes localized
T11221 23968-23970 IN denotes to
T11222 23971-23974 DT denotes the
T11223 23982-23989 NN denotes surface
T11224 23975-23981 JJ denotes apical
T11225 23990-23994 IN denotes upon
T11226 23995-24004 NN denotes forskolin
T11227 24005-24016 NN denotes stimulation
T11228 24017-24024 IN denotes whether
T11229 24044-24055 VBN denotes transfected
T11230 24025-24027 PRP denotes it
T11231 24028-24031 VBD denotes was
T11232 24032-24043 RB denotes transiently
T11233 24056-24060 IN denotes into
T11234 24061-24067 NN denotes vector
T11235 24068-24072 JJ denotes only
T11236 24067-24068 HYPH denotes -
T11237 24073-24078 NNS denotes cells
T11238 24079-24080 -LRB- denotes (
T11239 24102-24108 NNS denotes images
T11240 24080-24086 NN denotes Figure
T11241 24087-24089 CD denotes 5C
T11242 24089-24091 , denotes ,
T11243 24091-24096 JJ denotes upper
T11244 24097-24101 JJ denotes left
T11245 24108-24109 -RRB- denotes )
T11246 24110-24112 CC denotes or
T11247 24113-24117 IN denotes into
T11248 24118-24122 JJ denotes wild
T11249 24123-24127 NN denotes type
T11250 24122-24123 HYPH denotes -
T11251 24133-24138 NNS denotes cells
T11252 24128-24132 NN denotes AQP2
T11253 24139-24140 -LRB- denotes (
T11254 24140-24146 NN denotes Figure
T11255 24147-24149 CD denotes 5C
T11256 24149-24151 , denotes ,
T11257 24151-24156 JJ denotes upper
T11258 24157-24162 JJ denotes right
T11259 24162-24163 -RRB- denotes )
T11260 24163-24164 . denotes .
T11261 24164-24320 sentence denotes AQP2-F204V-GFP, when expressed by transient transfection into vector only cells, showed a diffuse cytoplasmic distribution pattern (Figure 5C, lower left).
T11262 24165-24169 NN denotes AQP2
T11263 24176-24179 NN denotes GFP
T11264 24169-24170 HYPH denotes -
T11265 24170-24175 NN denotes F204V
T11266 24175-24176 HYPH denotes -
T11267 24246-24252 VBD denotes showed
T11268 24179-24181 , denotes ,
T11269 24181-24185 WRB denotes when
T11270 24186-24195 VBN denotes expressed
T11271 24196-24198 IN denotes by
T11272 24199-24208 JJ denotes transient
T11273 24209-24221 NN denotes transfection
T11274 24222-24226 IN denotes into
T11275 24227-24233 NN denotes vector
T11276 24234-24238 JJ denotes only
T11277 24239-24244 NNS denotes cells
T11278 24244-24246 , denotes ,
T11279 24253-24254 DT denotes a
T11280 24288-24295 NN denotes pattern
T11281 24255-24262 JJ denotes diffuse
T11282 24263-24274 JJ denotes cytoplasmic
T11283 24275-24287 NN denotes distribution
T11293 24321-24325 WRB denotes When
T11294 24326-24335 VBN denotes expressed
T11295 24385-24394 VBD denotes localized
T11296 24336-24338 IN denotes in
T11297 24339-24343 JJ denotes wild
T11298 24344-24348 NN denotes type
T11299 24343-24344 HYPH denotes -
T11300 24354-24359 NNS denotes cells
T11301 24349-24353 NN denotes AQP2
T11302 24359-24361 , denotes ,
T11303 24361-24368 RB denotes however
T11304 24368-24370 , denotes ,
T11305 24370-24374 NN denotes AQP2
T11306 24381-24384 NN denotes GFP
T11307 24374-24375 HYPH denotes -
T11308 24375-24380 NN denotes F204V
T11309 24380-24381 HYPH denotes -
T11310 24395-24397 IN denotes to
T11311 24398-24401 DT denotes the
T11312 24409-24416 NN denotes surface
T11313 24402-24408 JJ denotes apical
T11314 24417-24419 IN denotes to
T11315 24420-24427 VBG denotes varying
T11316 24428-24435 NNS denotes degrees
T11317 24436-24437 -LRB- denotes (
T11318 24460-24466 NNS denotes images
T11319 24437-24443 NN denotes Figure
T11320 24444-24446 CD denotes 5C
T11321 24446-24448 , denotes ,
T11322 24448-24453 JJ denotes lower
T11323 24454-24459 JJ denotes right
T11324 24467-24468 -LRB- denotes [
T11325 24468-24469 CD denotes i
T11326 24469-24470 SYM denotes –
T11327 24470-24473 CD denotes iii
T11328 24473-24474 -RRB- denotes ]
T11329 24474-24475 -RRB- denotes )
T11330 24475-24476 . denotes .
T11331 24476-24558 sentence denotes The lower right images of Figure 5C shows three cells from a single transfection.
T11332 24477-24480 DT denotes The
T11333 24493-24499 NNS denotes images
T11334 24481-24486 JJ denotes lower
T11335 24487-24492 JJ denotes right
T11336 24513-24518 VBZ denotes shows
T11337 24500-24502 IN denotes of
T11338 24503-24509 NN denotes Figure
T11339 24510-24512 CD denotes 5C
T11340 24519-24524 CD denotes three
T11341 24525-24530 NNS denotes cells
T11342 24531-24535 IN denotes from
T11343 24536-24537 DT denotes a
T11344 24545-24557 NN denotes transfection
T11345 24538-24544 JJ denotes single
T11346 24557-24558 . denotes .
T11347 24558-24706 sentence denotes The first is a nontransfected cell that shows the localization of the stably expressing wild-type AQP2, which is apical upon forskolin stimulation.
T11348 24559-24562 DT denotes The
T11349 24563-24568 JJ denotes first
T11350 24569-24571 VBZ denotes is
T11351 24572-24573 DT denotes a
T11352 24589-24593 NN denotes cell
T11353 24574-24588 JJ denotes nontransfected
T11354 24594-24598 WDT denotes that
T11355 24599-24604 VBZ denotes shows
T11356 24605-24608 DT denotes the
T11357 24609-24621 NN denotes localization
T11358 24622-24624 IN denotes of
T11359 24625-24628 DT denotes the
T11360 24657-24661 NN denotes AQP2
T11361 24629-24635 RB denotes stably
T11362 24636-24646 VBG denotes expressing
T11363 24647-24651 JJ denotes wild
T11364 24652-24656 NN denotes type
T11365 24651-24652 HYPH denotes -
T11366 24661-24663 , denotes ,
T11367 24663-24668 WDT denotes which
T11368 24669-24671 VBZ denotes is
T11369 24672-24678 JJ denotes apical
T11370 24679-24683 IN denotes upon
T11371 24684-24693 NN denotes forskolin
T11372 24694-24705 NN denotes stimulation
T11373 24705-24706 . denotes .
T11374 24706-24803 sentence denotes The next two show expression of both the stable wild-type AQP2 and the transient AQP2-F204V-GFP.
T11375 24707-24710 DT denotes The
T11376 24716-24719 CD denotes two
T11377 24711-24715 JJ denotes next
T11378 24720-24724 VBP denotes show
T11379 24725-24735 NN denotes expression
T11380 24736-24738 IN denotes of
T11381 24739-24743 CC denotes both
T11382 24765-24769 NN denotes AQP2
T11383 24744-24747 DT denotes the
T11384 24748-24754 JJ denotes stable
T11385 24755-24759 JJ denotes wild
T11386 24760-24764 NN denotes type
T11387 24759-24760 HYPH denotes -
T11388 24770-24773 CC denotes and
T11389 24774-24777 DT denotes the
T11390 24799-24802 NN denotes GFP
T11391 24778-24787 JJ denotes transient
T11392 24788-24792 NN denotes AQP2
T11393 24792-24793 HYPH denotes -
T11394 24793-24798 NN denotes F204V
T11395 24798-24799 HYPH denotes -
T11396 24802-24803 . denotes .
T11397 24803-24936 sentence denotes In cell (ii), localization of wild-type AQP2 was indistinguishable from AQP2-F204V-GFP; both were apical upon forskolin stimulation.
T11398 24804-24806 IN denotes In
T11399 24849-24852 VBD denotes was
T11400 24807-24811 NN denotes cell
T11401 24812-24813 -LRB- denotes (
T11402 24813-24815 CD denotes ii
T11403 24815-24816 -RRB- denotes )
T11404 24816-24818 , denotes ,
T11405 24818-24830 NN denotes localization
T11406 24831-24833 IN denotes of
T11407 24834-24838 JJ denotes wild
T11408 24839-24843 NN denotes type
T11409 24838-24839 HYPH denotes -
T11410 24844-24848 NN denotes AQP2
T11411 24897-24901 VBD denotes were
T11412 24853-24870 JJ denotes indistinguishable
T11413 24871-24875 IN denotes from
T11414 24876-24880 NN denotes AQP2
T11415 24887-24890 NN denotes GFP
T11416 24880-24881 HYPH denotes -
T11417 24881-24886 NN denotes F204V
T11418 24886-24887 HYPH denotes -
T11419 24890-24891 : denotes ;
T11420 24892-24896 DT denotes both
T11421 24902-24908 JJ denotes apical
T11422 24909-24913 IN denotes upon
T11423 24914-24923 NN denotes forskolin
T11424 24924-24935 NN denotes stimulation
T11425 24935-24936 . denotes .
T11426 24936-25041 sentence denotes Although the effect was subtle in cell (iii), AQP2-F204V-GFP was partly localized to the apical surface.
T11427 24937-24945 IN denotes Although
T11428 24957-24960 VBD denotes was
T11429 24946-24949 DT denotes the
T11430 24950-24956 NN denotes effect
T11431 25009-25018 VBN denotes localized
T11432 24961-24967 JJ denotes subtle
T11433 24968-24970 IN denotes in
T11434 24971-24975 NN denotes cell
T11435 24976-24977 -LRB- denotes (
T11436 24977-24980 CD denotes iii
T11437 24980-24981 -RRB- denotes )
T11438 24981-24983 , denotes ,
T11439 24983-24987 NN denotes AQP2
T11440 24994-24997 NN denotes GFP
T11441 24987-24988 HYPH denotes -
T11442 24988-24993 NN denotes F204V
T11443 24993-24994 HYPH denotes -
T11444 24998-25001 VBD denotes was
T11445 25002-25008 RB denotes partly
T11446 25019-25021 IN denotes to
T11447 25022-25025 DT denotes the
T11448 25033-25040 NN denotes surface
T11449 25026-25032 JJ denotes apical
T11450 25040-25041 . denotes .
T11451 25041-25151 sentence denotes Generally, the localization of AQP2-F204V-GFP was clearly more apical when wild-type AQP2 was also expressed.
T11452 25042-25051 RB denotes Generally
T11453 25088-25091 VBD denotes was
T11454 25051-25053 , denotes ,
T11455 25053-25056 DT denotes the
T11456 25057-25069 NN denotes localization
T11457 25070-25072 IN denotes of
T11458 25073-25077 NN denotes AQP2
T11459 25084-25087 NN denotes GFP
T11460 25077-25078 HYPH denotes -
T11461 25078-25083 NN denotes F204V
T11462 25083-25084 HYPH denotes -
T11463 25092-25099 RB denotes clearly
T11464 25100-25104 RBR denotes more
T11465 25105-25111 JJ denotes apical
T11466 25112-25116 WRB denotes when
T11467 25141-25150 VBN denotes expressed
T11468 25117-25121 JJ denotes wild
T11469 25122-25126 NN denotes type
T11470 25121-25122 HYPH denotes -
T11471 25127-25131 NN denotes AQP2
T11472 25132-25135 VBD denotes was
T11473 25136-25140 RB denotes also
T11474 25150-25151 . denotes .
T11475 25151-25384 sentence denotes To confirm these results biochemically, we transfected the same cell lines (wild-type AQP2 or vector) with F204V-GFP, biotinylated surface proteins after forskolin stimulation, and precipitated the biotinylated proteins (Figure 5D).
T11476 25152-25154 TO denotes To
T11477 25155-25162 VB denotes confirm
T11478 25195-25206 VBD denotes transfected
T11479 25163-25168 DT denotes these
T11480 25169-25176 NNS denotes results
T11481 25177-25190 RB denotes biochemically
T11482 25190-25192 , denotes ,
T11483 25192-25194 PRP denotes we
T11484 25207-25210 DT denotes the
T11485 25221-25226 NNS denotes lines
T11486 25211-25215 JJ denotes same
T11487 25216-25220 NN denotes cell
T11488 25227-25228 -LRB- denotes (
T11489 25228-25232 JJ denotes wild
T11490 25233-25237 NN denotes type
T11491 25232-25233 HYPH denotes -
T11492 25238-25242 NN denotes AQP2
T11493 25243-25245 CC denotes or
T11494 25246-25252 NN denotes vector
T11495 25252-25253 -RRB- denotes )
T11496 25254-25258 IN denotes with
T11497 25259-25264 NN denotes F204V
T11498 25265-25268 NN denotes GFP
T11499 25264-25265 HYPH denotes -
T11500 25268-25270 , denotes ,
T11501 25270-25282 VBD denotes biotinylated
T11502 25283-25290 NN denotes surface
T11503 25291-25299 NN denotes proteins
T11504 25300-25305 IN denotes after
T11505 25306-25315 NN denotes forskolin
T11506 25316-25327 NN denotes stimulation
T11507 25327-25329 , denotes ,
T11508 25329-25332 CC denotes and
T11509 25333-25345 VBD denotes precipitated
T11510 25346-25349 DT denotes the
T11511 25363-25371 NN denotes proteins
T11512 25350-25362 VBN denotes biotinylated
T11513 25372-25373 -LRB- denotes (
T11514 25373-25379 NN denotes Figure
T11515 25380-25382 CD denotes 5D
T11516 25382-25383 -RRB- denotes )
T11517 25383-25384 . denotes .
T11518 25384-25577 sentence denotes AQP2-F204V-GFP is expressed approximately equally in both cell lines (Figure 5D, total cells), but is biotinylated only when wild-type AQP2 is also expressed (Figure 5D, surface biotinylated).
T11519 25385-25389 NN denotes AQP2
T11520 25396-25399 NN denotes GFP
T11521 25389-25390 HYPH denotes -
T11522 25390-25395 NN denotes F204V
T11523 25395-25396 HYPH denotes -
T11524 25403-25412 VBN denotes expressed
T11525 25400-25402 VBZ denotes is
T11526 25413-25426 RB denotes approximately
T11527 25427-25434 RB denotes equally
T11528 25435-25437 IN denotes in
T11529 25438-25442 DT denotes both
T11530 25448-25453 NNS denotes lines
T11531 25443-25447 NN denotes cell
T11532 25454-25455 -LRB- denotes (
T11533 25472-25477 NNS denotes cells
T11534 25455-25461 NN denotes Figure
T11535 25462-25464 CD denotes 5D
T11536 25464-25466 , denotes ,
T11537 25466-25471 JJ denotes total
T11538 25477-25478 -RRB- denotes )
T11539 25478-25480 , denotes ,
T11540 25480-25483 CC denotes but
T11541 25484-25486 VBZ denotes is
T11542 25487-25499 VBN denotes biotinylated
T11543 25500-25504 RB denotes only
T11544 25533-25542 VBN denotes expressed
T11545 25505-25509 WRB denotes when
T11546 25510-25514 JJ denotes wild
T11547 25515-25519 NN denotes type
T11548 25514-25515 HYPH denotes -
T11549 25520-25524 NN denotes AQP2
T11550 25525-25527 VBZ denotes is
T11551 25528-25532 RB denotes also
T11552 25543-25544 -LRB- denotes (
T11553 25544-25550 NN denotes Figure
T11554 25551-25553 CD denotes 5D
T11555 25553-25555 , denotes ,
T11556 25555-25562 NN denotes surface
T11557 25563-25575 VBN denotes biotinylated
T11558 25575-25576 -RRB- denotes )
T11559 25576-25577 . denotes .
T11560 25577-25762 sentence denotes Since only cell surface proteins are accessible to biotin, these results indicate that AQP2-F204V is transported to the cell surface when wild-type AQP2 is present, but not on its own.
T11561 25578-25583 IN denotes Since
T11562 25611-25614 VBP denotes are
T11563 25584-25588 RB denotes only
T11564 25602-25610 NN denotes proteins
T11565 25589-25593 NN denotes cell
T11566 25594-25601 NN denotes surface
T11567 25651-25659 VBP denotes indicate
T11568 25615-25625 JJ denotes accessible
T11569 25626-25628 IN denotes to
T11570 25629-25635 NN denotes biotin
T11571 25635-25637 , denotes ,
T11572 25637-25642 DT denotes these
T11573 25643-25650 NNS denotes results
T11574 25660-25664 IN denotes that
T11575 25679-25690 VBN denotes transported
T11576 25665-25669 NN denotes AQP2
T11577 25670-25675 NN denotes F204V
T11578 25669-25670 HYPH denotes -
T11579 25676-25678 VBZ denotes is
T11580 25691-25693 IN denotes to
T11581 25694-25697 DT denotes the
T11582 25703-25710 NN denotes surface
T11583 25698-25702 NN denotes cell
T11584 25711-25715 WRB denotes when
T11585 25731-25733 VBZ denotes is
T11586 25716-25720 JJ denotes wild
T11587 25721-25725 NN denotes type
T11588 25720-25721 HYPH denotes -
T11589 25726-25730 NN denotes AQP2
T11590 25734-25741 JJ denotes present
T11591 25741-25743 , denotes ,
T11592 25743-25746 CC denotes but
T11593 25747-25750 RB denotes not
T11594 25751-25753 IN denotes on
T11595 25754-25757 PRP$ denotes its
T11596 25758-25761 JJ denotes own
T11597 25761-25762 . denotes .
T14172 25775-25783 NN denotes Aqp2F204
T14173 25784-25789 NN denotes F204V
T14174 25783-25784 HYPH denotes /
T14175 25790-25794 NNS denotes mice
T14176 25795-25798 VBP denotes are
T14177 25799-25805 JJ denotes viable
T14178 25806-25809 CC denotes and
T14179 25810-25814 VBP denotes grow
T14180 25815-25818 CC denotes and
T14181 25819-25828 VBP denotes reproduce
T14182 25829-25837 RB denotes normally
T14183 25837-25838 . denotes .
T14184 25838-26037 sentence denotes They are, however, severely defective in their ability to concentrate urine, leading to increased urine output and water intake, thus making them the first mouse model of NDI to survive to maturity.
T14185 25839-25843 PRP denotes They
T14186 25844-25847 VBP denotes are
T14187 25847-25849 , denotes ,
T14188 25849-25856 RB denotes however
T14189 25856-25858 , denotes ,
T14190 25858-25866 RB denotes severely
T14191 25867-25876 JJ denotes defective
T14192 25877-25879 IN denotes in
T14193 25880-25885 PRP$ denotes their
T14194 25886-25893 NN denotes ability
T14195 25894-25896 TO denotes to
T14196 25897-25908 VB denotes concentrate
T14197 25909-25914 NN denotes urine
T14198 25914-25916 , denotes ,
T14199 25916-25923 VBG denotes leading
T14200 25924-25926 IN denotes to
T14201 25927-25936 VBN denotes increased
T14202 25943-25949 NN denotes output
T14203 25937-25942 NN denotes urine
T14204 25950-25953 CC denotes and
T14205 25954-25959 NN denotes water
T14206 25960-25966 NN denotes intake
T14207 25966-25968 , denotes ,
T14208 25968-25972 RB denotes thus
T14209 25973-25979 VBG denotes making
T14210 25980-25984 PRP denotes them
T14211 26001-26006 NN denotes model
T14212 25985-25988 DT denotes the
T14213 25989-25994 JJ denotes first
T14214 25995-26000 NN denotes mouse
T14215 26007-26009 IN denotes of
T14216 26010-26013 NN denotes NDI
T14217 26014-26016 TO denotes to
T14218 26017-26024 VB denotes survive
T14219 26025-26027 IN denotes to
T14220 26028-26036 NN denotes maturity
T14221 26036-26037 . denotes .
T14222 26037-26093 sentence denotes In humans, NDI is caused by mutations in Avpr2 or Aqp2.
T14223 26038-26040 IN denotes In
T14224 26056-26062 VBN denotes caused
T14225 26041-26047 NNS denotes humans
T14226 26047-26049 , denotes ,
T14227 26049-26052 NN denotes NDI
T14228 26053-26055 VBZ denotes is
T14229 26063-26065 IN denotes by
T14230 26066-26075 NNS denotes mutations
T14231 26076-26078 IN denotes in
T14232 26079-26084 NN denotes Avpr2
T14233 26085-26087 CC denotes or
T14234 26088-26092 NN denotes Aqp2
T14235 26092-26093 . denotes .
T14236 26093-26288 sentence denotes Knockout of the X-linked Avpr2 gene in mice [15] gave an NDI-like phenotype in male, hemizygous neonates, but the phenotype could not be assessed in adults as the mice died within 1 wk of birth.
T14237 26094-26102 NN denotes Knockout
T14238 26143-26147 VBD denotes gave
T14239 26103-26105 IN denotes of
T14240 26106-26109 DT denotes the
T14241 26125-26129 NN denotes gene
T14242 26110-26111 NN denotes X
T14243 26112-26118 VBN denotes linked
T14244 26111-26112 HYPH denotes -
T14245 26119-26124 NN denotes Avpr2
T14246 26130-26132 IN denotes in
T14247 26133-26137 NNS denotes mice
T14248 26138-26139 -LRB- denotes [
T14249 26139-26141 CD denotes 15
T14250 26141-26142 -RRB- denotes ]
T14251 26148-26150 DT denotes an
T14252 26160-26169 NN denotes phenotype
T14253 26151-26154 NN denotes NDI
T14254 26155-26159 JJ denotes like
T14255 26154-26155 HYPH denotes -
T14256 26170-26172 IN denotes in
T14257 26173-26177 JJ denotes male
T14258 26190-26198 NNS denotes neonates
T14259 26177-26179 , denotes ,
T14260 26179-26189 JJ denotes hemizygous
T14261 26198-26200 , denotes ,
T14262 26200-26203 CC denotes but
T14263 26204-26207 DT denotes the
T14264 26208-26217 NN denotes phenotype
T14265 26231-26239 VBN denotes assessed
T14266 26218-26223 MD denotes could
T14267 26224-26227 RB denotes not
T14268 26228-26230 VB denotes be
T14269 26240-26242 IN denotes in
T14270 26243-26249 NNS denotes adults
T14271 26250-26252 IN denotes as
T14272 26262-26266 VBD denotes died
T14273 26253-26256 DT denotes the
T14274 26257-26261 NNS denotes mice
T14275 26267-26273 IN denotes within
T14276 26274-26275 CD denotes 1
T14277 26276-26278 NN denotes wk
T14278 26279-26281 IN denotes of
T14279 26282-26287 NN denotes birth
T14280 26287-26288 . denotes .
T14281 26288-26423 sentence denotes The adult heterozygous females showed a mild tendency toward increased urinary output and water intake and decreased urine osmolality.
T14282 26289-26292 DT denotes The
T14283 26312-26319 NNS denotes females
T14284 26293-26298 JJ denotes adult
T14285 26299-26311 JJ denotes heterozygous
T14286 26320-26326 VBD denotes showed
T14287 26327-26328 DT denotes a
T14288 26334-26342 NN denotes tendency
T14289 26329-26333 JJ denotes mild
T14290 26343-26349 IN denotes toward
T14291 26350-26359 VBN denotes increased
T14292 26368-26374 NN denotes output
T14293 26360-26367 JJ denotes urinary
T14294 26375-26378 CC denotes and
T14295 26379-26384 NN denotes water
T14296 26385-26391 NN denotes intake
T14297 26392-26395 CC denotes and
T14298 26396-26405 VBN denotes decreased
T14299 26412-26422 NN denotes osmolality
T14300 26406-26411 NN denotes urine
T14301 26422-26423 . denotes .
T14302 26423-26478 sentence denotes Knockout of the mouse Aqp2 gene has not been reported.
T14303 26424-26432 NN denotes Knockout
T14304 26469-26477 VBN denotes reported
T14305 26433-26435 IN denotes of
T14306 26436-26439 DT denotes the
T14307 26451-26455 NN denotes gene
T14308 26440-26445 NN denotes mouse
T14309 26446-26450 NN denotes Aqp2
T14310 26456-26459 VBZ denotes has
T14311 26460-26463 RB denotes not
T14312 26464-26468 VBN denotes been
T14313 26477-26478 . denotes .
T14314 26478-26563 sentence denotes A knock-in of a human disease-causing mutation (T126M), however, has been made [18].
T14315 26479-26480 DT denotes A
T14316 26487-26489 NN denotes in
T14317 26481-26486 NN denotes knock
T14318 26486-26487 HYPH denotes -
T14319 26553-26557 VBN denotes made
T14320 26490-26492 IN denotes of
T14321 26493-26494 DT denotes a
T14322 26517-26525 NN denotes mutation
T14323 26495-26500 JJ denotes human
T14324 26501-26508 NN denotes disease
T14325 26509-26516 VBG denotes causing
T14326 26508-26509 HYPH denotes -
T14327 26526-26527 -LRB- denotes (
T14328 26527-26532 NN denotes T126M
T14329 26532-26533 -RRB- denotes )
T14330 26533-26535 , denotes ,
T14331 26535-26542 RB denotes however
T14332 26542-26544 , denotes ,
T14333 26544-26547 VBZ denotes has
T14334 26548-26552 VBN denotes been
T14335 26558-26559 -LRB- denotes [
T14336 26559-26561 CD denotes 18
T14337 26561-26562 -RRB- denotes ]
T14338 26562-26563 . denotes .
T14339 26563-26672 sentence denotes These mice have a severe urine-concentrating defect resulting in dehydration and death within 1 wk of birth.
T14340 26564-26569 DT denotes These
T14341 26570-26574 NNS denotes mice
T14342 26575-26579 VBP denotes have
T14343 26580-26581 DT denotes a
T14344 26609-26615 NN denotes defect
T14345 26582-26588 JJ denotes severe
T14346 26589-26594 NN denotes urine
T14347 26595-26608 VBG denotes concentrating
T14348 26594-26595 HYPH denotes -
T14349 26616-26625 VBG denotes resulting
T14350 26626-26628 IN denotes in
T14351 26629-26640 NN denotes dehydration
T14352 26641-26644 CC denotes and
T14353 26645-26650 NN denotes death
T14354 26651-26657 IN denotes within
T14355 26658-26659 CD denotes 1
T14356 26660-26662 NN denotes wk
T14357 26663-26665 IN denotes of
T14358 26666-26671 NN denotes birth
T14359 26671-26672 . denotes .
T14360 26672-26748 sentence denotes Curiously, AQP2-T126M does localize properly in at least a subset of cells.
T14361 26673-26682 RB denotes Curiously
T14362 26700-26708 VB denotes localize
T14363 26682-26684 , denotes ,
T14364 26684-26688 NN denotes AQP2
T14365 26689-26694 NN denotes T126M
T14366 26688-26689 HYPH denotes -
T14367 26695-26699 VBZ denotes does
T14368 26709-26717 RB denotes properly
T14369 26718-26720 IN denotes in
T14370 26721-26723 RB denotes at
T14371 26724-26729 RBS denotes least
T14372 26732-26738 NN denotes subset
T14373 26730-26731 DT denotes a
T14374 26739-26741 IN denotes of
T14375 26742-26747 NNS denotes cells
T14376 26747-26748 . denotes .
T14377 26748-26873 sentence denotes The grossly abnormal collecting duct morphology makes it impossible to pinpoint the molecular defect in these knock-in mice.
T14378 26749-26752 DT denotes The
T14379 26786-26796 NN denotes morphology
T14380 26753-26760 RB denotes grossly
T14381 26761-26769 JJ denotes abnormal
T14382 26770-26780 VBG denotes collecting
T14383 26781-26785 NN denotes duct
T14384 26797-26802 VBZ denotes makes
T14385 26803-26805 PRP denotes it
T14386 26806-26816 JJ denotes impossible
T14387 26817-26819 TO denotes to
T14388 26820-26828 VB denotes pinpoint
T14389 26829-26832 DT denotes the
T14390 26843-26849 NN denotes defect
T14391 26833-26842 JJ denotes molecular
T14392 26850-26852 IN denotes in
T14393 26853-26858 DT denotes these
T14394 26868-26872 NNS denotes mice
T14395 26859-26864 VB denotes knock
T14396 26864-26865 HYPH denotes -
T14397 26865-26867 RP denotes in
T14398 26872-26873 . denotes .
T14399 26873-26943 sentence denotes The T126M knock-in clearly shows that Aqp2 is an essential gene [18].
T14400 26874-26877 DT denotes The
T14401 26890-26892 NN denotes in
T14402 26878-26883 NN denotes T126M
T14403 26884-26889 NN denotes knock
T14404 26889-26890 HYPH denotes -
T14405 26901-26906 VBZ denotes shows
T14406 26893-26900 RB denotes clearly
T14407 26907-26911 IN denotes that
T14408 26917-26919 VBZ denotes is
T14409 26912-26916 NN denotes Aqp2
T14410 26920-26922 DT denotes an
T14411 26933-26937 NN denotes gene
T14412 26923-26932 JJ denotes essential
T14413 26938-26939 -LRB- denotes [
T14414 26939-26941 CD denotes 18
T14415 26941-26942 -RRB- denotes ]
T14416 26942-26943 . denotes .
T14417 26943-27121 sentence denotes The fact that our mice survive shows either that AQP2-F204V possesses some residual water transporting ability or that there are AVP-independent pathways for water reabsorption.
T14418 26944-26947 DT denotes The
T14419 26948-26952 NN denotes fact
T14420 26975-26980 VBZ denotes shows
T14421 26953-26957 IN denotes that
T14422 26967-26974 VBP denotes survive
T14423 26958-26961 PRP$ denotes our
T14424 26962-26966 NNS denotes mice
T14425 26981-26987 CC denotes either
T14426 27004-27013 VBZ denotes possesses
T14427 26988-26992 IN denotes that
T14428 26993-26997 NN denotes AQP2
T14429 26998-27003 NN denotes F204V
T14430 26997-26998 HYPH denotes -
T14431 27014-27018 DT denotes some
T14432 27047-27054 NN denotes ability
T14433 27019-27027 JJ denotes residual
T14434 27028-27033 NN denotes water
T14435 27034-27046 VBG denotes transporting
T14436 27055-27057 CC denotes or
T14437 27058-27062 IN denotes that
T14438 27069-27072 VBP denotes are
T14439 27063-27068 EX denotes there
T14440 27073-27076 NN denotes AVP
T14441 27077-27088 JJ denotes independent
T14442 27076-27077 HYPH denotes -
T14443 27089-27097 NNS denotes pathways
T14444 27098-27101 IN denotes for
T14445 27102-27107 NN denotes water
T14446 27108-27120 NN denotes reabsorption
T14447 27120-27121 . denotes .
T14448 27121-27279 sentence denotes Residual activity of AQP2-F204V is likely, as mutant animals show some small response to dDAVP, although dDAVP-stimulated urine osmolality remains quite low.
T14449 27122-27130 JJ denotes Residual
T14450 27131-27139 NN denotes activity
T14451 27154-27156 VBZ denotes is
T14452 27140-27142 IN denotes of
T14453 27143-27147 NN denotes AQP2
T14454 27148-27153 NN denotes F204V
T14455 27147-27148 HYPH denotes -
T14456 27157-27163 JJ denotes likely
T14457 27163-27165 , denotes ,
T14458 27165-27167 IN denotes as
T14459 27183-27187 VBP denotes show
T14460 27168-27174 NN denotes mutant
T14461 27175-27182 NNS denotes animals
T14462 27188-27192 DT denotes some
T14463 27199-27207 NN denotes response
T14464 27193-27198 JJ denotes small
T14465 27208-27210 IN denotes to
T14466 27211-27216 NN denotes dDAVP
T14467 27216-27218 , denotes ,
T14468 27218-27226 IN denotes although
T14469 27261-27268 VBZ denotes remains
T14470 27227-27232 NN denotes dDAVP
T14471 27233-27243 VBN denotes stimulated
T14472 27232-27233 HYPH denotes -
T14473 27250-27260 NN denotes osmolality
T14474 27244-27249 NN denotes urine
T14475 27269-27274 RB denotes quite
T14476 27275-27278 JJ denotes low
T14477 27278-27279 . denotes .
T14478 27279-27443 sentence denotes Immunostaining of kidney shows that AQP2-F204V does not efficiently transport water, because it fails to localize to the apical cell surface after dDAVP treatment.
T14479 27280-27294 NN denotes Immunostaining
T14480 27305-27310 VBZ denotes shows
T14481 27295-27297 IN denotes of
T14482 27298-27304 NN denotes kidney
T14483 27311-27315 IN denotes that
T14484 27348-27357 VB denotes transport
T14485 27316-27320 NN denotes AQP2
T14486 27321-27326 NN denotes F204V
T14487 27320-27321 HYPH denotes -
T14488 27327-27331 VBZ denotes does
T14489 27332-27335 RB denotes not
T14490 27336-27347 RB denotes efficiently
T14491 27358-27363 NN denotes water
T14492 27363-27365 , denotes ,
T14493 27365-27372 IN denotes because
T14494 27376-27381 VBZ denotes fails
T14495 27373-27375 PRP denotes it
T14496 27382-27384 TO denotes to
T14497 27385-27393 VB denotes localize
T14498 27394-27396 IN denotes to
T14499 27397-27400 DT denotes the
T14500 27413-27420 NN denotes surface
T14501 27401-27407 JJ denotes apical
T14502 27408-27412 NN denotes cell
T14503 27421-27426 IN denotes after
T14504 27427-27432 NN denotes dDAVP
T14505 27433-27442 NN denotes treatment
T14506 27442-27443 . denotes .
T14507 27443-27578 sentence denotes Some residual activity of AQP2 would imply that some small, undetectable portion of the mutant protein is getting to the cell surface.
T14508 27444-27448 DT denotes Some
T14509 27458-27466 NN denotes activity
T14510 27449-27457 JJ denotes residual
T14511 27481-27486 VB denotes imply
T14512 27467-27469 IN denotes of
T14513 27470-27474 NN denotes AQP2
T14514 27475-27480 MD denotes would
T14515 27487-27491 IN denotes that
T14516 27550-27557 VBG denotes getting
T14517 27492-27496 DT denotes some
T14518 27517-27524 NN denotes portion
T14519 27497-27502 JJ denotes small
T14520 27502-27504 , denotes ,
T14521 27504-27516 JJ denotes undetectable
T14522 27525-27527 IN denotes of
T14523 27528-27531 DT denotes the
T14524 27539-27546 NN denotes protein
T14525 27532-27538 NN denotes mutant
T14526 27547-27549 VBZ denotes is
T14527 27558-27560 IN denotes to
T14528 27561-27564 DT denotes the
T14529 27570-27577 NN denotes surface
T14530 27565-27569 NN denotes cell
T14531 27577-27578 . denotes .
T14532 27578-27741 sentence denotes The surface biotinylation experiment (Figure 5D) suggests that no mutant protein gets to the surface, but this does not necessarily reflect the situation in vivo.
T14533 27579-27582 DT denotes The
T14534 27605-27615 NN denotes experiment
T14535 27583-27590 NN denotes surface
T14536 27591-27604 NN denotes biotinylation
T14537 27628-27636 VBZ denotes suggests
T14538 27616-27617 -LRB- denotes (
T14539 27617-27623 NN denotes Figure
T14540 27624-27626 CD denotes 5D
T14541 27626-27627 -RRB- denotes )
T14542 27637-27641 IN denotes that
T14543 27660-27664 VBZ denotes gets
T14544 27642-27644 DT denotes no
T14545 27652-27659 NN denotes protein
T14546 27645-27651 NN denotes mutant
T14547 27665-27667 IN denotes to
T14548 27668-27671 DT denotes the
T14549 27672-27679 NN denotes surface
T14550 27679-27681 , denotes ,
T14551 27681-27684 CC denotes but
T14552 27685-27689 DT denotes this
T14553 27711-27718 VB denotes reflect
T14554 27690-27694 VBZ denotes does
T14555 27695-27698 RB denotes not
T14556 27699-27710 RB denotes necessarily
T14557 27719-27722 DT denotes the
T14558 27723-27732 NN denotes situation
T14559 27733-27735 FW denotes in
T14560 27736-27740 FW denotes vivo
T14561 27740-27741 . denotes .
T14562 27741-27933 sentence denotes While this small fraction of protein may not be detectable by immunofluorescence, Western blotting shows that some mutant protein does progress beyond the ER (35–45 kDa species in Figure 3A).
T14563 27742-27747 IN denotes While
T14564 27787-27789 VB denotes be
T14565 27748-27752 DT denotes this
T14566 27759-27767 NN denotes fraction
T14567 27753-27758 JJ denotes small
T14568 27768-27770 IN denotes of
T14569 27771-27778 NN denotes protein
T14570 27779-27782 MD denotes may
T14571 27783-27786 RB denotes not
T14572 27841-27846 VBZ denotes shows
T14573 27790-27800 JJ denotes detectable
T14574 27801-27803 IN denotes by
T14575 27804-27822 NN denotes immunofluorescence
T14576 27822-27824 , denotes ,
T14577 27824-27831 NNP denotes Western
T14578 27832-27840 NN denotes blotting
T14579 27847-27851 IN denotes that
T14580 27877-27885 VB denotes progress
T14581 27852-27856 DT denotes some
T14582 27864-27871 NN denotes protein
T14583 27857-27863 NN denotes mutant
T14584 27872-27876 VBZ denotes does
T14585 27886-27892 IN denotes beyond
T14586 27893-27896 DT denotes the
T14587 27897-27899 NN denotes ER
T14588 27900-27901 -LRB- denotes (
T14589 27911-27918 NNS denotes species
T14590 27901-27903 CD denotes 35
T14591 27904-27906 CD denotes 45
T14592 27903-27904 SYM denotes –
T14593 27907-27910 NN denotes kDa
T14594 27919-27921 IN denotes in
T14595 27922-27928 NN denotes Figure
T14596 27929-27931 CD denotes 3A
T14597 27931-27932 -RRB- denotes )
T14598 27932-27933 . denotes .
T14599 27933-28122 sentence denotes Compared to wild-type, mutant protein is enriched in the high-mannose, core-glycosylated form (31 kDa) and deficient in nonglycosylated (29 kDa) and complex glycosylated (35–45 kDa) forms.
T14600 27934-27942 VBN denotes Compared
T14601 27972-27974 VBZ denotes is
T14602 27943-27945 IN denotes to
T14603 27946-27950 JJ denotes wild
T14604 27951-27955 NN denotes type
T14605 27950-27951 HYPH denotes -
T14606 27955-27957 , denotes ,
T14607 27957-27963 NN denotes mutant
T14608 27964-27971 NN denotes protein
T14609 27975-27983 VBN denotes enriched
T14610 27984-27986 IN denotes in
T14611 27987-27990 DT denotes the
T14612 28023-28027 NN denotes form
T14613 27991-27995 JJ denotes high
T14614 27996-28003 NN denotes mannose
T14615 27995-27996 HYPH denotes -
T14616 28003-28005 , denotes ,
T14617 28005-28009 NN denotes core
T14618 28010-28022 VBN denotes glycosylated
T14619 28009-28010 HYPH denotes -
T14620 28028-28029 -LRB- denotes (
T14621 28032-28035 NN denotes kDa
T14622 28029-28031 CD denotes 31
T14623 28035-28036 -RRB- denotes )
T14624 28037-28040 CC denotes and
T14625 28041-28050 JJ denotes deficient
T14626 28051-28053 IN denotes in
T14627 28054-28069 JJ denotes nonglycosylated
T14628 28116-28121 NNS denotes forms
T14629 28070-28071 -LRB- denotes (
T14630 28074-28077 NN denotes kDa
T14631 28071-28073 CD denotes 29
T14632 28077-28078 -RRB- denotes )
T14633 28079-28082 CC denotes and
T14634 28083-28090 JJ denotes complex
T14635 28091-28103 JJ denotes glycosylated
T14636 28104-28105 -LRB- denotes (
T14637 28111-28114 NN denotes kDa
T14638 28105-28107 CD denotes 35
T14639 28108-28110 CD denotes 45
T14640 28107-28108 SYM denotes –
T14641 28114-28115 -RRB- denotes )
T14642 28121-28122 . denotes .
T14643 28122-28301 sentence denotes The presence of a reduced but detectable amount of protein in the 35–45 kDa range indicates that mutant protein is transported out of the ER, but with greatly reduced efficiency.
T14644 28123-28126 DT denotes The
T14645 28127-28135 NN denotes presence
T14646 28205-28214 VBZ denotes indicates
T14647 28136-28138 IN denotes of
T14648 28139-28140 DT denotes a
T14649 28164-28170 NN denotes amount
T14650 28141-28148 VBN denotes reduced
T14651 28149-28152 CC denotes but
T14652 28153-28163 JJ denotes detectable
T14653 28171-28173 IN denotes of
T14654 28174-28181 NN denotes protein
T14655 28182-28184 IN denotes in
T14656 28185-28188 DT denotes the
T14657 28199-28204 NN denotes range
T14658 28189-28191 CD denotes 35
T14659 28192-28194 CD denotes 45
T14660 28191-28192 SYM denotes –
T14661 28195-28198 NN denotes kDa
T14662 28215-28219 IN denotes that
T14663 28238-28249 VBN denotes transported
T14664 28220-28226 NN denotes mutant
T14665 28227-28234 NN denotes protein
T14666 28235-28237 VBZ denotes is
T14667 28250-28253 IN denotes out
T14668 28254-28256 IN denotes of
T14669 28257-28260 DT denotes the
T14670 28261-28263 NN denotes ER
T14671 28263-28265 , denotes ,
T14672 28265-28268 CC denotes but
T14673 28269-28273 IN denotes with
T14674 28274-28281 RB denotes greatly
T14675 28282-28289 VBN denotes reduced
T14676 28290-28300 NN denotes efficiency
T14677 28300-28301 . denotes .
T14678 28301-28486 sentence denotes Colocalization of AQP2-F204V with the ER protein calnexin in transfected MDCK cells shows that, while most of the mutant protein is trapped in the ER, some does progress beyond the ER.
T14679 28302-28316 NN denotes Colocalization
T14680 28386-28391 VBZ denotes shows
T14681 28317-28319 IN denotes of
T14682 28320-28324 NN denotes AQP2
T14683 28325-28330 NN denotes F204V
T14684 28324-28325 HYPH denotes -
T14685 28331-28335 IN denotes with
T14686 28336-28339 DT denotes the
T14687 28343-28350 NN denotes protein
T14688 28340-28342 NN denotes ER
T14689 28351-28359 NN denotes calnexin
T14690 28360-28362 IN denotes in
T14691 28363-28374 VBN denotes transfected
T14692 28380-28385 NNS denotes cells
T14693 28375-28379 NN denotes MDCK
T14694 28392-28396 IN denotes that
T14695 28463-28471 VB denotes progress
T14696 28396-28398 , denotes ,
T14697 28398-28403 IN denotes while
T14698 28434-28441 VBN denotes trapped
T14699 28404-28408 JJS denotes most
T14700 28409-28411 IN denotes of
T14701 28412-28415 DT denotes the
T14702 28423-28430 NN denotes protein
T14703 28416-28422 NN denotes mutant
T14704 28431-28433 VBZ denotes is
T14705 28442-28444 IN denotes in
T14706 28445-28448 DT denotes the
T14707 28449-28451 NN denotes ER
T14708 28451-28453 , denotes ,
T14709 28453-28457 DT denotes some
T14710 28458-28462 VBZ denotes does
T14711 28472-28478 IN denotes beyond
T14712 28479-28482 DT denotes the
T14713 28483-28485 NN denotes ER
T14714 28485-28486 . denotes .
T14715 28486-28804 sentence denotes Diminished response to dDAVP, diminished abundance of mature glycosylated protein in mutant animals, and the transport of a fraction of mutant protein beyond the ER in MDCK cells are all consistent with the notion that AQP2-F204V misfolding is limited and that it may retain some residual water transporting activity.
T14716 28487-28497 VBN denotes Diminished
T14717 28498-28506 NN denotes response
T14718 28666-28669 VBP denotes are
T14719 28507-28509 IN denotes to
T14720 28510-28515 NN denotes dDAVP
T14721 28515-28517 , denotes ,
T14722 28517-28527 VBN denotes diminished
T14723 28528-28537 NN denotes abundance
T14724 28538-28540 IN denotes of
T14725 28541-28547 JJ denotes mature
T14726 28561-28568 NN denotes protein
T14727 28548-28560 VBN denotes glycosylated
T14728 28569-28571 IN denotes in
T14729 28572-28578 NN denotes mutant
T14730 28579-28586 NNS denotes animals
T14731 28586-28588 , denotes ,
T14732 28588-28591 CC denotes and
T14733 28592-28595 DT denotes the
T14734 28596-28605 NN denotes transport
T14735 28606-28608 IN denotes of
T14736 28609-28610 DT denotes a
T14737 28611-28619 NN denotes fraction
T14738 28620-28622 IN denotes of
T14739 28623-28629 NN denotes mutant
T14740 28630-28637 NN denotes protein
T14741 28638-28644 IN denotes beyond
T14742 28645-28648 DT denotes the
T14743 28649-28651 NN denotes ER
T14744 28652-28654 IN denotes in
T14745 28655-28659 NN denotes MDCK
T14746 28660-28665 NNS denotes cells
T14747 28670-28673 RB denotes all
T14748 28674-28684 JJ denotes consistent
T14749 28685-28689 IN denotes with
T14750 28690-28693 DT denotes the
T14751 28694-28700 NN denotes notion
T14752 28701-28705 IN denotes that
T14753 28728-28730 VBZ denotes is
T14754 28706-28710 NN denotes AQP2
T14755 28711-28716 NN denotes F204V
T14756 28710-28711 HYPH denotes -
T14757 28717-28727 NN denotes misfolding
T14758 28731-28738 JJ denotes limited
T14759 28739-28742 CC denotes and
T14760 28743-28747 IN denotes that
T14761 28755-28761 VB denotes retain
T14762 28748-28750 PRP denotes it
T14763 28751-28754 MD denotes may
T14764 28762-28766 DT denotes some
T14765 28795-28803 NN denotes activity
T14766 28767-28775 JJ denotes residual
T14767 28776-28781 NN denotes water
T14768 28782-28794 VBG denotes transporting
T14769 28803-28804 . denotes .
T14770 28804-28899 sentence denotes Evidently this residual activity is sufficient for the viability and growth of mutant animals.
T14771 28805-28814 RB denotes Evidently
T14772 28838-28840 VBZ denotes is
T14773 28815-28819 DT denotes this
T14774 28829-28837 NN denotes activity
T14775 28820-28828 JJ denotes residual
T14776 28841-28851 JJ denotes sufficient
T14777 28852-28855 IN denotes for
T14778 28856-28859 DT denotes the
T14779 28860-28869 NN denotes viability
T14780 28870-28873 CC denotes and
T14781 28874-28880 NN denotes growth
T14782 28881-28883 IN denotes of
T14783 28884-28890 NN denotes mutant
T14784 28891-28898 NNS denotes animals
T14785 28898-28899 . denotes .
T14786 28899-29021 sentence denotes Reduced efficiency in exiting the ER may explain why AQP2-F204V is enriched in the 31 kDa high-mannose glycosylated form.
T14787 28900-28907 VBN denotes Reduced
T14788 28908-28918 NN denotes efficiency
T14789 28941-28948 VB denotes explain
T14790 28919-28921 IN denotes in
T14791 28922-28929 VBG denotes exiting
T14792 28930-28933 DT denotes the
T14793 28934-28936 NN denotes ER
T14794 28937-28940 MD denotes may
T14795 28949-28952 WRB denotes why
T14796 28967-28975 VBN denotes enriched
T14797 28953-28957 NN denotes AQP2
T14798 28958-28963 NN denotes F204V
T14799 28957-28958 HYPH denotes -
T14800 28964-28966 VBZ denotes is
T14801 28976-28978 IN denotes in
T14802 28979-28982 DT denotes the
T14803 29016-29020 NN denotes form
T14804 28983-28985 CD denotes 31
T14805 28986-28989 NN denotes kDa
T14806 28990-28994 JJ denotes high
T14807 28995-29002 NN denotes mannose
T14808 28994-28995 HYPH denotes -
T14809 29003-29015 VBN denotes glycosylated
T14810 29020-29021 . denotes .
T14811 29021-29144 sentence denotes The high-mannose core oligosaccharide is added in the ER and is later modified and elaborated in the Golgi apparatus [26].
T14812 29022-29025 DT denotes The
T14813 29044-29059 NN denotes oligosaccharide
T14814 29026-29030 JJ denotes high
T14815 29031-29038 NN denotes mannose
T14816 29030-29031 HYPH denotes -
T14817 29039-29043 NN denotes core
T14818 29063-29068 VBN denotes added
T14819 29060-29062 VBZ denotes is
T14820 29069-29071 IN denotes in
T14821 29072-29075 DT denotes the
T14822 29076-29078 NN denotes ER
T14823 29079-29082 CC denotes and
T14824 29083-29085 VBZ denotes is
T14825 29092-29100 VBN denotes modified
T14826 29086-29091 RB denotes later
T14827 29101-29104 CC denotes and
T14828 29105-29115 VBN denotes elaborated
T14829 29116-29118 IN denotes in
T14830 29119-29122 DT denotes the
T14831 29129-29138 NN denotes apparatus
T14832 29123-29128 NN denotes Golgi
T14833 29139-29140 -LRB- denotes [
T14834 29140-29142 CD denotes 26
T14835 29142-29143 -RRB- denotes ]
T14836 29143-29144 . denotes .
T14837 29144-29305 sentence denotes The increase in the high-mannose glycosylated form of AQP2-F204V may simply reflect its prolonged presence in the ER and exposure to oligosaccharyl transferase.
T14838 29145-29148 DT denotes The
T14839 29149-29157 NN denotes increase
T14840 29221-29228 VB denotes reflect
T14841 29158-29160 IN denotes in
T14842 29161-29164 DT denotes the
T14843 29191-29195 NN denotes form
T14844 29165-29169 JJ denotes high
T14845 29170-29177 NN denotes mannose
T14846 29169-29170 HYPH denotes -
T14847 29178-29190 VBN denotes glycosylated
T14848 29196-29198 IN denotes of
T14849 29199-29203 NN denotes AQP2
T14850 29204-29209 NN denotes F204V
T14851 29203-29204 HYPH denotes -
T14852 29210-29213 MD denotes may
T14853 29214-29220 RB denotes simply
T14854 29229-29232 PRP$ denotes its
T14855 29243-29251 NN denotes presence
T14856 29233-29242 JJ denotes prolonged
T14857 29252-29254 IN denotes in
T14858 29255-29258 DT denotes the
T14859 29259-29261 NN denotes ER
T14860 29262-29265 CC denotes and
T14861 29266-29274 NN denotes exposure
T14862 29275-29277 IN denotes to
T14863 29278-29292 NN denotes oligosaccharyl
T14864 29293-29304 NN denotes transferase
T14865 29304-29305 . denotes .
T14866 29305-29475 sentence denotes While improper localization of AQP2 explains the phenotype of homozygous mutant mice, the complete lack of a phenotype in heterozygous mice is more difficult to explain.
T14867 29306-29311 IN denotes While
T14868 29342-29350 VBZ denotes explains
T14869 29312-29320 JJ denotes improper
T14870 29321-29333 NN denotes localization
T14871 29334-29336 IN denotes of
T14872 29337-29341 NN denotes AQP2
T14873 29446-29448 VBZ denotes is
T14874 29351-29354 DT denotes the
T14875 29355-29364 NN denotes phenotype
T14876 29365-29367 IN denotes of
T14877 29368-29378 JJ denotes homozygous
T14878 29386-29390 NNS denotes mice
T14879 29379-29385 NN denotes mutant
T14880 29390-29392 , denotes ,
T14881 29392-29395 DT denotes the
T14882 29405-29409 NN denotes lack
T14883 29396-29404 JJ denotes complete
T14884 29410-29412 IN denotes of
T14885 29413-29414 DT denotes a
T14886 29415-29424 NN denotes phenotype
T14887 29425-29427 IN denotes in
T14888 29428-29440 JJ denotes heterozygous
T14889 29441-29445 NNS denotes mice
T14890 29449-29453 RBR denotes more
T14891 29454-29463 JJ denotes difficult
T14892 29464-29466 TO denotes to
T14893 29467-29474 VB denotes explain
T14894 29474-29475 . denotes .
T14895 29475-29632 sentence denotes Physiologically, heterozygous mice have no symptoms (see Figure 1C), and they are indistinguishable from wild type on immunostaining of kidneys (Figure 5A).
T14896 29476-29491 RB denotes Physiologically
T14897 29511-29515 VBP denotes have
T14898 29491-29493 , denotes ,
T14899 29493-29505 JJ denotes heterozygous
T14900 29506-29510 NNS denotes mice
T14901 29516-29518 DT denotes no
T14902 29519-29527 NNS denotes symptoms
T14903 29528-29529 -LRB- denotes (
T14904 29529-29532 VB denotes see
T14905 29533-29539 NN denotes Figure
T14906 29540-29542 CD denotes 1C
T14907 29542-29543 -RRB- denotes )
T14908 29543-29545 , denotes ,
T14909 29545-29548 CC denotes and
T14910 29549-29553 PRP denotes they
T14911 29554-29557 VBP denotes are
T14912 29558-29575 JJ denotes indistinguishable
T14913 29576-29580 IN denotes from
T14914 29581-29585 JJ denotes wild
T14915 29586-29590 NN denotes type
T14916 29591-29593 IN denotes on
T14917 29594-29608 NN denotes immunostaining
T14918 29609-29611 IN denotes of
T14919 29612-29619 NNS denotes kidneys
T14920 29620-29621 -LRB- denotes (
T14921 29621-29627 NN denotes Figure
T14922 29628-29630 CD denotes 5A
T14923 29630-29631 -RRB- denotes )
T14924 29631-29632 . denotes .
T14925 29632-29794 sentence denotes The presence of 50% of the normal amount of wild-type protein may explain the lack of symptoms, but it cannot explain the lack of any ER-retained mutant protein.
T14926 29633-29636 DT denotes The
T14927 29637-29645 NN denotes presence
T14928 29699-29706 VB denotes explain
T14929 29646-29648 IN denotes of
T14930 29649-29651 CD denotes 50
T14931 29651-29652 NN denotes %
T14932 29653-29655 IN denotes of
T14933 29656-29659 DT denotes the
T14934 29667-29673 NN denotes amount
T14935 29660-29666 JJ denotes normal
T14936 29674-29676 IN denotes of
T14937 29677-29681 JJ denotes wild
T14938 29682-29686 NN denotes type
T14939 29681-29682 HYPH denotes -
T14940 29687-29694 NN denotes protein
T14941 29695-29698 MD denotes may
T14942 29707-29710 DT denotes the
T14943 29711-29715 NN denotes lack
T14944 29716-29718 IN denotes of
T14945 29719-29727 NNS denotes symptoms
T14946 29727-29729 , denotes ,
T14947 29729-29732 CC denotes but
T14948 29733-29735 PRP denotes it
T14949 29743-29750 VB denotes explain
T14950 29736-29739 MD denotes can
T14951 29739-29742 RB denotes not
T14952 29751-29754 DT denotes the
T14953 29755-29759 NN denotes lack
T14954 29760-29762 IN denotes of
T14955 29763-29766 DT denotes any
T14956 29786-29793 NN denotes protein
T14957 29767-29769 NN denotes ER
T14958 29770-29778 VBN denotes retained
T14959 29769-29770 HYPH denotes -
T14960 29779-29785 NN denotes mutant
T14961 29793-29794 . denotes .
T14962 29794-29919 sentence denotes Rather, the phenotype of the Aqp2F204V/+ animals suggests that the mutant protein is being rescued by the wild-type protein.
T14963 29795-29801 RB denotes Rather
T14964 29844-29852 VBZ denotes suggests
T14965 29801-29803 , denotes ,
T14966 29803-29806 DT denotes the
T14967 29807-29816 NN denotes phenotype
T14968 29817-29819 IN denotes of
T14969 29820-29823 DT denotes the
T14970 29836-29843 NNS denotes animals
T14971 29824-29833 NN denotes Aqp2F204V
T14972 29833-29834 HYPH denotes /
T14973 29834-29835 SYM denotes +
T14974 29853-29857 IN denotes that
T14975 29886-29893 VBN denotes rescued
T14976 29858-29861 DT denotes the
T14977 29869-29876 NN denotes protein
T14978 29862-29868 NN denotes mutant
T14979 29877-29879 VBZ denotes is
T14980 29880-29885 VBG denotes being
T14981 29894-29896 IN denotes by
T14982 29897-29900 DT denotes the
T14983 29911-29918 NN denotes protein
T14984 29901-29905 JJ denotes wild
T14985 29906-29910 NN denotes type
T14986 29905-29906 HYPH denotes -
T14987 29918-29919 . denotes .
T14988 29919-30149 sentence denotes Indeed, de Mattia et al. (26) have demonstrated that one recessive allele of Aqp2, P262L, does not properly translocate when expressed by itself in MDCK cells, but that in the presence of wild-type protein, it localizes normally.
T14989 29920-29926 RB denotes Indeed
T14990 29955-29967 VBN denotes demonstrated
T14991 29926-29928 , denotes ,
T14992 29928-29930 NNP denotes de
T14993 29931-29937 NNP denotes Mattia
T14994 29938-29940 FW denotes et
T14995 29941-29944 FW denotes al.
T14996 29945-29946 -LRB- denotes (
T14997 29946-29948 CD denotes 26
T14998 29948-29949 -RRB- denotes )
T14999 29950-29954 VBP denotes have
T15000 29968-29972 IN denotes that
T15001 30028-30039 VB denotes translocate
T15002 29973-29976 CD denotes one
T15003 29987-29993 NN denotes allele
T15004 29977-29986 JJ denotes recessive
T15005 29994-29996 IN denotes of
T15006 29997-30001 NN denotes Aqp2
T15007 30001-30003 , denotes ,
T15008 30003-30008 NN denotes P262L
T15009 30008-30010 , denotes ,
T15010 30010-30014 VBZ denotes does
T15011 30015-30018 RB denotes not
T15012 30019-30027 RB denotes properly
T15013 30040-30044 WRB denotes when
T15014 30045-30054 VBN denotes expressed
T15015 30055-30057 IN denotes by
T15016 30058-30064 PRP denotes itself
T15017 30065-30067 IN denotes in
T15018 30068-30072 NN denotes MDCK
T15019 30073-30078 NNS denotes cells
T15020 30078-30080 , denotes ,
T15021 30080-30083 CC denotes but
T15022 30084-30088 IN denotes that
T15023 30130-30139 VBZ denotes localizes
T15024 30089-30091 IN denotes in
T15025 30092-30095 DT denotes the
T15026 30096-30104 NN denotes presence
T15027 30105-30107 IN denotes of
T15028 30108-30112 JJ denotes wild
T15029 30113-30117 NN denotes type
T15030 30112-30113 HYPH denotes -
T15031 30118-30125 NN denotes protein
T15032 30125-30127 , denotes ,
T15033 30127-30129 PRP denotes it
T15034 30140-30148 RB denotes normally
T15035 30148-30149 . denotes .
T15036 30149-30214 sentence denotes The same mechanism seems to apply in vivo with Aqp2F204V/+ mice.
T15037 30150-30153 DT denotes The
T15038 30159-30168 NN denotes mechanism
T15039 30154-30158 JJ denotes same
T15040 30169-30174 VBZ denotes seems
T15041 30175-30177 TO denotes to
T15042 30178-30183 VB denotes apply
T15043 30184-30186 FW denotes in
T15044 30187-30191 FW denotes vivo
T15045 30192-30196 IN denotes with
T15046 30197-30206 NN denotes Aqp2F204V
T15047 30209-30213 NNS denotes mice
T15048 30206-30207 HYPH denotes /
T15049 30207-30208 SYM denotes +
T15050 30213-30214 . denotes .
T15051 30214-30413 sentence denotes In support of this, AQP2-F204V can interact with wild-type AQP2 (Figure 5B), and when coexpressed with wild-type protein, AQP2-F204V can reach the cell surface (Figure 5C bottom right panel and 5D).
T15052 30215-30217 IN denotes In
T15053 30250-30258 VB denotes interact
T15054 30218-30225 NN denotes support
T15055 30226-30228 IN denotes of
T15056 30229-30233 DT denotes this
T15057 30233-30235 , denotes ,
T15058 30235-30239 NN denotes AQP2
T15059 30240-30245 NN denotes F204V
T15060 30239-30240 HYPH denotes -
T15061 30246-30249 MD denotes can
T15062 30259-30263 IN denotes with
T15063 30264-30268 JJ denotes wild
T15064 30269-30273 NN denotes type
T15065 30268-30269 HYPH denotes -
T15066 30274-30278 NN denotes AQP2
T15067 30279-30280 -LRB- denotes (
T15068 30280-30286 NN denotes Figure
T15069 30287-30289 CD denotes 5B
T15070 30289-30290 -RRB- denotes )
T15071 30290-30292 , denotes ,
T15072 30292-30295 CC denotes and
T15073 30296-30300 WRB denotes when
T15074 30301-30312 VBN denotes coexpressed
T15075 30352-30357 VB denotes reach
T15076 30313-30317 IN denotes with
T15077 30318-30322 JJ denotes wild
T15078 30323-30327 NN denotes type
T15079 30322-30323 HYPH denotes -
T15080 30328-30335 NN denotes protein
T15081 30335-30337 , denotes ,
T15082 30337-30341 NN denotes AQP2
T15083 30342-30347 NN denotes F204V
T15084 30341-30342 HYPH denotes -
T15085 30348-30351 MD denotes can
T15086 30358-30361 DT denotes the
T15087 30367-30374 NN denotes surface
T15088 30362-30366 NN denotes cell
T15089 30375-30376 -LRB- denotes (
T15090 30399-30404 NN denotes panel
T15091 30376-30382 NN denotes Figure
T15092 30383-30385 CD denotes 5C
T15093 30386-30392 NN denotes bottom
T15094 30393-30398 JJ denotes right
T15095 30405-30408 CC denotes and
T15096 30409-30411 CD denotes 5D
T15097 30411-30412 -RRB- denotes )
T15098 30412-30413 . denotes .
T15099 30413-30715 sentence denotes Although it has been demonstrated that a recessive allele (encoding AQP2-R187C) of NDI fails to interact with wild-type AQP2 [6], here we show that AQP2-F204V does interact with the wild-type protein, presumably as part of heterotetramers, and represents a rescuable allele, both in vitro and in vivo.
T15100 30414-30422 IN denotes Although
T15101 30435-30447 VBN denotes demonstrated
T15102 30423-30425 PRP denotes it
T15103 30426-30429 VBZ denotes has
T15104 30430-30434 VBN denotes been
T15105 30552-30556 VBP denotes show
T15106 30448-30452 IN denotes that
T15107 30501-30506 VBZ denotes fails
T15108 30453-30454 DT denotes a
T15109 30465-30471 NN denotes allele
T15110 30455-30464 JJ denotes recessive
T15111 30472-30473 -LRB- denotes (
T15112 30473-30481 VBG denotes encoding
T15113 30482-30486 NN denotes AQP2
T15114 30487-30492 NN denotes R187C
T15115 30486-30487 HYPH denotes -
T15116 30492-30493 -RRB- denotes )
T15117 30494-30496 IN denotes of
T15118 30497-30500 NN denotes NDI
T15119 30507-30509 TO denotes to
T15120 30510-30518 VB denotes interact
T15121 30519-30523 IN denotes with
T15122 30524-30528 JJ denotes wild
T15123 30529-30533 NN denotes type
T15124 30528-30529 HYPH denotes -
T15125 30534-30538 NN denotes AQP2
T15126 30539-30540 -LRB- denotes [
T15127 30540-30541 CD denotes 6
T15128 30541-30542 -RRB- denotes ]
T15129 30542-30544 , denotes ,
T15130 30544-30548 RB denotes here
T15131 30549-30551 PRP denotes we
T15132 30557-30561 IN denotes that
T15133 30578-30586 VB denotes interact
T15134 30562-30566 NN denotes AQP2
T15135 30567-30572 NN denotes F204V
T15136 30566-30567 HYPH denotes -
T15137 30573-30577 VBZ denotes does
T15138 30587-30591 IN denotes with
T15139 30592-30595 DT denotes the
T15140 30606-30613 NN denotes protein
T15141 30596-30600 JJ denotes wild
T15142 30601-30605 NN denotes type
T15143 30600-30601 HYPH denotes -
T15144 30613-30615 , denotes ,
T15145 30615-30625 RB denotes presumably
T15146 30626-30628 IN denotes as
T15147 30629-30633 NN denotes part
T15148 30634-30636 IN denotes of
T15149 30637-30652 NNS denotes heterotetramers
T15150 30652-30654 , denotes ,
T15151 30654-30657 CC denotes and
T15152 30658-30668 VBZ denotes represents
T15153 30669-30670 DT denotes a
T15154 30681-30687 NN denotes allele
T15155 30671-30680 JJ denotes rescuable
T15156 30687-30689 , denotes ,
T15157 30689-30693 CC denotes both
T15158 30697-30702 FW denotes vitro
T15159 30694-30696 FW denotes in
T15160 30703-30706 CC denotes and
T15161 30707-30709 FW denotes in
T15162 30710-30714 FW denotes vivo
T15163 30714-30715 . denotes .
T15164 30715-30949 sentence denotes Immunostaining the kidneys of homozygous Aqp2F204V/F204V mice shows that the mutant-expressing collecting duct cells can not mediate water reabsorption, because it fails to insert into the apical plasma membrane in response to dDAVP.
T15165 30716-30730 VBG denotes Immunostaining
T15166 30778-30783 VBZ denotes shows
T15167 30731-30734 DT denotes the
T15168 30735-30742 NNS denotes kidneys
T15169 30743-30745 IN denotes of
T15170 30746-30756 JJ denotes homozygous
T15171 30773-30777 NNS denotes mice
T15172 30757-30766 NN denotes Aqp2F204V
T15173 30767-30772 NN denotes F204V
T15174 30766-30767 HYPH denotes /
T15175 30784-30788 IN denotes that
T15176 30841-30848 VB denotes mediate
T15177 30789-30792 DT denotes the
T15178 30827-30832 NNS denotes cells
T15179 30793-30799 NN denotes mutant
T15180 30800-30810 VBG denotes expressing
T15181 30799-30800 HYPH denotes -
T15182 30811-30821 VBG denotes collecting
T15183 30822-30826 NN denotes duct
T15184 30833-30836 MD denotes can
T15185 30837-30840 RB denotes not
T15186 30849-30854 NN denotes water
T15187 30855-30867 NN denotes reabsorption
T15188 30867-30869 , denotes ,
T15189 30869-30876 IN denotes because
T15190 30880-30885 VBZ denotes fails
T15191 30877-30879 PRP denotes it
T15192 30886-30888 TO denotes to
T15193 30889-30895 VB denotes insert
T15194 30896-30900 IN denotes into
T15195 30901-30904 DT denotes the
T15196 30919-30927 NN denotes membrane
T15197 30905-30911 JJ denotes apical
T15198 30912-30918 NN denotes plasma
T15199 30928-30930 IN denotes in
T15200 30931-30939 NN denotes response
T15201 30940-30942 IN denotes to
T15202 30943-30948 NN denotes dDAVP
T15203 30948-30949 . denotes .
T15204 30949-31076 sentence denotes This is this first in vivo proof of a long-standing hypothesis that comes from in vitro studies with recessive Aqp2 mutations.
T15205 30950-30954 DT denotes This
T15206 30955-30957 VBZ denotes is
T15207 30958-30962 DT denotes this
T15208 30977-30982 NN denotes proof
T15209 30963-30968 JJ denotes first
T15210 30969-30971 FW denotes in
T15211 30972-30976 FW denotes vivo
T15212 30983-30985 IN denotes of
T15213 30986-30987 DT denotes a
T15214 31002-31012 NN denotes hypothesis
T15215 30988-30992 JJ denotes long
T15216 30993-31001 VBG denotes standing
T15217 30992-30993 HYPH denotes -
T15218 31013-31017 WDT denotes that
T15219 31018-31023 VBZ denotes comes
T15220 31024-31028 IN denotes from
T15221 31029-31031 FW denotes in
T15222 31032-31037 FW denotes vitro
T15223 31038-31045 NNS denotes studies
T15224 31046-31050 IN denotes with
T15225 31051-31060 JJ denotes recessive
T15226 31066-31075 NNS denotes mutations
T15227 31061-31065 NN denotes Aqp2
T15228 31075-31076 . denotes .
T15229 31076-31295 sentence denotes Transfection into MDCK cells of any of several Aqp2 mutations corresponding to recessive human alleles shows abnormal subcellular localization [25], [27] and failure to appropriately translocate to the plasma membrane.
T15230 31077-31089 NN denotes Transfection
T15231 31180-31185 VBZ denotes shows
T15232 31090-31094 IN denotes into
T15233 31095-31099 NN denotes MDCK
T15234 31100-31105 NNS denotes cells
T15235 31106-31108 IN denotes of
T15236 31109-31112 DT denotes any
T15237 31113-31115 IN denotes of
T15238 31116-31123 JJ denotes several
T15239 31129-31138 NNS denotes mutations
T15240 31124-31128 NN denotes Aqp2
T15241 31139-31152 VBG denotes corresponding
T15242 31153-31155 IN denotes to
T15243 31156-31165 JJ denotes recessive
T15244 31172-31179 NNS denotes alleles
T15245 31166-31171 JJ denotes human
T15246 31186-31194 JJ denotes abnormal
T15247 31207-31219 NN denotes localization
T15248 31195-31206 JJ denotes subcellular
T15249 31220-31221 -LRB- denotes [
T15250 31221-31223 CD denotes 25
T15251 31223-31224 -RRB- denotes ]
T15252 31224-31226 , denotes ,
T15253 31226-31227 -LRB- denotes [
T15254 31227-31229 CD denotes 27
T15255 31229-31230 -RRB- denotes ]
T15256 31231-31234 CC denotes and
T15257 31235-31242 NN denotes failure
T15258 31243-31245 TO denotes to
T15259 31260-31271 VB denotes translocate
T15260 31246-31259 RB denotes appropriately
T15261 31272-31274 IN denotes to
T15262 31275-31278 DT denotes the
T15263 31286-31294 NN denotes membrane
T15264 31279-31285 NN denotes plasma
T15265 31294-31295 . denotes .
T15266 31295-31442 sentence denotes Thus, misfolding, retention in the ER, and failure to translocate in response to dDAVP were proposed as the mechanism for autosomal recessive NDI.
T15267 31296-31300 RB denotes Thus
T15268 31388-31396 VBN denotes proposed
T15269 31300-31302 , denotes ,
T15270 31302-31312 NN denotes misfolding
T15271 31312-31314 , denotes ,
T15272 31314-31323 NN denotes retention
T15273 31324-31326 IN denotes in
T15274 31327-31330 DT denotes the
T15275 31331-31333 NN denotes ER
T15276 31333-31335 , denotes ,
T15277 31335-31338 CC denotes and
T15278 31339-31346 NN denotes failure
T15279 31347-31349 TO denotes to
T15280 31350-31361 VB denotes translocate
T15281 31362-31364 IN denotes in
T15282 31365-31373 NN denotes response
T15283 31374-31376 IN denotes to
T15284 31377-31382 NN denotes dDAVP
T15285 31383-31387 VBD denotes were
T15286 31397-31399 IN denotes as
T15287 31400-31403 DT denotes the
T15288 31404-31413 NN denotes mechanism
T15289 31414-31417 IN denotes for
T15290 31418-31427 JJ denotes autosomal
T15291 31438-31441 NN denotes NDI
T15292 31428-31437 JJ denotes recessive
T15293 31441-31442 . denotes .
T15294 31442-31530 sentence denotes Here we not only prove this hypothesis but also establish a useful model for human NDI.
T15295 31443-31447 RB denotes Here
T15296 31460-31465 VB denotes prove
T15297 31448-31450 PRP denotes we
T15298 31451-31454 RB denotes not
T15299 31455-31459 RB denotes only
T15300 31466-31470 DT denotes this
T15301 31471-31481 NN denotes hypothesis
T15302 31482-31485 CC denotes but
T15303 31486-31490 RB denotes also
T15304 31491-31500 VB denotes establish
T15305 31501-31502 DT denotes a
T15306 31510-31515 NN denotes model
T15307 31503-31509 JJ denotes useful
T15308 31516-31519 IN denotes for
T15309 31520-31525 JJ denotes human
T15310 31526-31529 NN denotes NDI
T15311 31529-31530 . denotes .
T15312 31530-31716 sentence denotes This mouse model of NDI based on an Aqp2 allele that can be rescued provides the opportunity to test therapies, including gene therapy, that may promote proper subcellular localization.
T15313 31531-31535 DT denotes This
T15314 31542-31547 NN denotes model
T15315 31536-31541 NN denotes mouse
T15316 31599-31607 VBZ denotes provides
T15317 31548-31550 IN denotes of
T15318 31551-31554 NN denotes NDI
T15319 31555-31560 VBN denotes based
T15320 31561-31563 IN denotes on
T15321 31564-31566 DT denotes an
T15322 31572-31578 NN denotes allele
T15323 31567-31571 NN denotes Aqp2
T15324 31579-31583 WDT denotes that
T15325 31591-31598 VBN denotes rescued
T15326 31584-31587 MD denotes can
T15327 31588-31590 VB denotes be
T15328 31608-31611 DT denotes the
T15329 31612-31623 NN denotes opportunity
T15330 31624-31626 TO denotes to
T15331 31627-31631 VB denotes test
T15332 31632-31641 NNS denotes therapies
T15333 31641-31643 , denotes ,
T15334 31643-31652 VBG denotes including
T15335 31653-31657 NN denotes gene
T15336 31658-31665 NN denotes therapy
T15337 31665-31667 , denotes ,
T15338 31667-31671 WDT denotes that
T15339 31676-31683 VB denotes promote
T15340 31672-31675 MD denotes may
T15341 31684-31690 JJ denotes proper
T15342 31703-31715 NN denotes localization
T15343 31691-31702 JJ denotes subcellular
T15344 31715-31716 . denotes .
T15507 31741-31751 NN denotes Generation
T15508 31752-31754 IN denotes of
T15509 31755-31758 NN denotes ENU
T15510 31759-31763 NNS denotes mice
T15511 31764-31767 CC denotes and
T15512 31768-31775 NN denotes housing
T15513 31775-31776 . denotes .
T15514 31776-31839 sentence denotes ENU mutagenized C57BL/6 mice were generated as described [19].
T15515 31777-31780 NN denotes ENU
T15516 31801-31805 NNS denotes mice
T15517 31781-31792 VBN denotes mutagenized
T15518 31793-31798 NN denotes C57BL
T15519 31798-31799 HYPH denotes /
T15520 31799-31800 CD denotes 6
T15521 31811-31820 VBN denotes generated
T15522 31806-31810 VBD denotes were
T15523 31821-31823 IN denotes as
T15524 31824-31833 VBN denotes described
T15525 31834-31835 -LRB- denotes [
T15526 31835-31837 CD denotes 19
T15527 31837-31838 -RRB- denotes ]
T15528 31838-31839 . denotes .
T15529 31839-32055 sentence denotes Mice were maintained by backcrossing affected animals to C57BL/6 and housed in the Genomics Institute of the Novartis Research Foundation Specific Pathogen Free animal facility (La Jolla, California, United States).
T15530 31840-31844 NNS denotes Mice
T15531 31850-31860 VBN denotes maintained
T15532 31845-31849 VBD denotes were
T15533 31861-31863 IN denotes by
T15534 31864-31876 VBG denotes backcrossing
T15535 31877-31885 VBN denotes affected
T15536 31886-31893 NNS denotes animals
T15537 31894-31896 IN denotes to
T15538 31897-31902 NN denotes C57BL
T15539 31902-31903 HYPH denotes /
T15540 31903-31904 CD denotes 6
T15541 31905-31908 CC denotes and
T15542 31909-31915 VBN denotes housed
T15543 31916-31918 IN denotes in
T15544 31919-31922 DT denotes the
T15545 31932-31941 NNP denotes Institute
T15546 31923-31931 NNP denotes Genomics
T15547 31942-31944 IN denotes of
T15548 31945-31948 DT denotes the
T15549 32008-32016 NNP denotes facility
T15550 31949-31957 NNP denotes Novartis
T15551 31967-31977 NNP denotes Foundation
T15552 31958-31966 NNP denotes Research
T15553 31978-31986 NNP denotes Specific
T15554 31987-31995 NNP denotes Pathogen
T15555 31996-32000 NNP denotes Free
T15556 32001-32007 NNP denotes animal
T15557 32017-32018 -LRB- denotes (
T15558 32021-32026 NNP denotes Jolla
T15559 32018-32020 NNP denotes La
T15560 32026-32028 , denotes ,
T15561 32028-32038 NNP denotes California
T15562 32038-32040 , denotes ,
T15563 32040-32046 NNP denotes United
T15564 32047-32053 NNP denotes States
T15565 32053-32054 -RRB- denotes )
T15566 32054-32055 . denotes .
T15567 32055-32191 sentence denotes All procedures were approved by the Genomics Institute of the Novartis Research Foundation Institutional Animal Care and Use Committee.
T15568 32056-32059 DT denotes All
T15569 32060-32070 NNS denotes procedures
T15570 32076-32084 VBN denotes approved
T15571 32071-32075 VBD denotes were
T15572 32085-32087 IN denotes by
T15573 32088-32091 DT denotes the
T15574 32101-32110 NNP denotes Institute
T15575 32092-32100 NNP denotes Genomics
T15576 32111-32113 IN denotes of
T15577 32114-32117 DT denotes the
T15578 32181-32190 NNP denotes Committee
T15579 32118-32126 NNP denotes Novartis
T15580 32136-32146 NNP denotes Foundation
T15581 32127-32135 NNP denotes Research
T15582 32147-32160 NNP denotes Institutional
T15583 32161-32167 NNP denotes Animal
T15584 32168-32172 NNP denotes Care
T15585 32173-32176 CC denotes and
T15586 32177-32180 NNP denotes Use
T15587 32190-32191 . denotes .
T17314 34883-34893 NN denotes homogenate
T15900 32193-32203 NNS denotes Constructs
T15901 32203-32204 . denotes .
T15902 32204-32406 sentence denotes The complete coding sequence of mouse AQP2 from an IMAGE clone was digested from the pCMV⋅SPORT6 plasmid with EcoRI and NotI and ligated into pcDNA3.1 (Invitrogen, Carlsbad, California, United States).
T15903 32205-32208 DT denotes The
T15904 32225-32233 NN denotes sequence
T15905 32209-32217 JJ denotes complete
T15906 32218-32224 NN denotes coding
T15907 32272-32280 VBN denotes digested
T15908 32234-32236 IN denotes of
T15909 32237-32242 NN denotes mouse
T15910 32243-32247 NN denotes AQP2
T15911 32248-32252 IN denotes from
T15912 32253-32255 DT denotes an
T15913 32262-32267 NN denotes clone
T15914 32256-32261 NN denotes IMAGE
T15915 32268-32271 VBD denotes was
T15916 32281-32285 IN denotes from
T15917 32286-32289 DT denotes the
T15918 32302-32309 NN denotes plasmid
T15919 32290-32301 NN denotes pCMV⋅SPORT6
T15920 32310-32314 IN denotes with
T15921 32315-32320 NN denotes EcoRI
T15922 32321-32324 CC denotes and
T15923 32325-32329 NN denotes NotI
T15924 32330-32333 CC denotes and
T15925 32334-32341 VBN denotes ligated
T15926 32342-32346 IN denotes into
T15927 32347-32355 NN denotes pcDNA3.1
T15928 32356-32357 -LRB- denotes (
T15929 32357-32367 NNP denotes Invitrogen
T15930 32367-32369 , denotes ,
T15931 32369-32377 NNP denotes Carlsbad
T15932 32377-32379 , denotes ,
T15933 32379-32389 NNP denotes California
T15934 32389-32391 , denotes ,
T15935 32391-32397 NNP denotes United
T15936 32398-32404 NNP denotes States
T15937 32404-32405 -RRB- denotes )
T15938 32405-32406 . denotes .
T15939 32406-32660 sentence denotes The F204V mutation was introduced by site-directed mutagenesis (Stratagene, La Jolla, California, United States), using the sense oligonucleotide 5′-GATGATCACTGGGTCGTCTGGATCGGACCCC-3′, and antisense oligonucleotide 5′-GGGGTCCGATCCAGACGACCCAGTGATCATC-3′.
T15940 32407-32410 DT denotes The
T15941 32417-32425 NN denotes mutation
T15942 32411-32416 NN denotes F204V
T15943 32430-32440 VBN denotes introduced
T15944 32426-32429 VBD denotes was
T15945 32441-32443 IN denotes by
T15946 32444-32448 NN denotes site
T15947 32449-32457 JJ denotes directed
T15948 32448-32449 HYPH denotes -
T15949 32458-32469 NN denotes mutagenesis
T15950 32470-32471 -LRB- denotes (
T15951 32471-32481 NNP denotes Stratagene
T15952 32481-32483 , denotes ,
T15953 32483-32485 NNP denotes La
T15954 32486-32491 NNP denotes Jolla
T15955 32491-32493 , denotes ,
T15956 32493-32503 NNP denotes California
T15957 32503-32505 , denotes ,
T15958 32505-32511 NNP denotes United
T15959 32512-32518 NNP denotes States
T15960 32518-32519 -RRB- denotes )
T15961 32519-32521 , denotes ,
T15962 32521-32526 VBG denotes using
T15963 32527-32530 DT denotes the
T15964 32537-32552 NN denotes oligonucleotide
T15965 32531-32536 NN denotes sense
T15966 32553-32554 CD denotes 5
T15967 32556-32587 NN denotes GATGATCACTGGGTCGTCTGGATCGGACCCC
T15968 32554-32555 SYM denotes ′
T15969 32555-32556 HYPH denotes -
T15970 32587-32588 HYPH denotes -
T15971 32588-32589 CD denotes 3
T15972 32589-32590 SYM denotes ′
T15973 32590-32592 , denotes ,
T15974 32592-32595 CC denotes and
T15975 32596-32605 JJ denotes antisense
T15976 32606-32621 NN denotes oligonucleotide
T15977 32622-32623 CD denotes 5
T15978 32625-32656 NN denotes GGGGTCCGATCCAGACGACCCAGTGATCATC
T15979 32623-32624 SYM denotes ′
T15980 32624-32625 HYPH denotes -
T15981 32656-32657 HYPH denotes -
T15982 32657-32658 CD denotes 3
T15983 32658-32659 SYM denotes ′
T15984 32659-32660 . denotes .
T15985 32660-32849 sentence denotes To generate GFP fusions of AQP2, the pCMV⋅SPORT6 AQP2 construct was used in a PCR reaction with the primers Sp6 and 5′-GACTGGATCCCGGCCTTGCTGCCGCGCGGCAG-3′ to remove the stop codon of AQP2.
T15986 32661-32663 TO denotes To
T15987 32664-32672 VB denotes generate
T15988 32729-32733 VBN denotes used
T15989 32673-32676 NN denotes GFP
T15990 32677-32684 NNS denotes fusions
T15991 32685-32687 IN denotes of
T15992 32688-32692 NN denotes AQP2
T15993 32692-32694 , denotes ,
T15994 32694-32697 DT denotes the
T15995 32715-32724 NN denotes construct
T15996 32698-32709 NN denotes pCMV⋅SPORT6
T15997 32710-32714 NN denotes AQP2
T15998 32725-32728 VBD denotes was
T15999 32734-32736 IN denotes in
T16000 32737-32738 DT denotes a
T16001 32743-32751 NN denotes reaction
T16002 32739-32742 NN denotes PCR
T16003 32752-32756 IN denotes with
T16004 32757-32760 DT denotes the
T16005 32769-32772 NN denotes Sp6
T16006 32761-32768 NNS denotes primers
T16007 32773-32776 CC denotes and
T16008 32777-32778 CD denotes 5
T16009 32780-32812 NN denotes GACTGGATCCCGGCCTTGCTGCCGCGCGGCAG
T16010 32778-32779 SYM denotes ′
T16011 32779-32780 HYPH denotes -
T16012 32812-32813 HYPH denotes -
T16013 32813-32814 CD denotes 3
T16014 32814-32815 SYM denotes ′
T16015 32816-32818 TO denotes to
T16016 32819-32825 VB denotes remove
T16017 32826-32829 DT denotes the
T16018 32835-32840 NN denotes codon
T16019 32830-32834 NN denotes stop
T16020 32841-32843 IN denotes of
T16021 32844-32848 NN denotes AQP2
T16022 32848-32849 . denotes .
T16023 32849-32976 sentence denotes The product was digested with KpnI and BamHI and ligated into pEGFP-N2 (BD Biosciences, San Diego, California, United States).
T16024 32850-32853 DT denotes The
T16025 32854-32861 NN denotes product
T16026 32866-32874 VBN denotes digested
T16027 32862-32865 VBD denotes was
T16028 32875-32879 IN denotes with
T16029 32880-32884 NN denotes KpnI
T16030 32885-32888 CC denotes and
T16031 32889-32894 NN denotes BamHI
T16032 32895-32898 CC denotes and
T16033 32899-32906 VBN denotes ligated
T16034 32907-32911 IN denotes into
T16035 32912-32917 NN denotes pEGFP
T16036 32918-32920 NN denotes N2
T16037 32917-32918 HYPH denotes -
T16038 32921-32922 -LRB- denotes (
T16039 32925-32936 NNP denotes Biosciences
T16040 32922-32924 NN denotes BD
T16041 32936-32938 , denotes ,
T16042 32938-32941 NNP denotes San
T16043 32942-32947 NNP denotes Diego
T16044 32947-32949 , denotes ,
T16045 32949-32959 NNP denotes California
T16046 32959-32961 , denotes ,
T16047 32961-32967 NNP denotes United
T16048 32968-32974 NNP denotes States
T16049 32974-32975 -RRB- denotes )
T16050 32975-32976 . denotes .
T16051 32976-33053 sentence denotes The F204V mutation was introduced using the same mutagenic oligonucleotides.
T16052 32977-32980 DT denotes The
T16053 32987-32995 NN denotes mutation
T16054 32981-32986 NN denotes F204V
T16055 33000-33010 VBN denotes introduced
T16056 32996-32999 VBD denotes was
T16057 33011-33016 VBG denotes using
T16058 33017-33020 DT denotes the
T16059 33036-33052 NNS denotes oligonucleotides
T16060 33021-33025 JJ denotes same
T16061 33026-33035 JJ denotes mutagenic
T16062 33052-33053 . denotes .
T16399 33055-33059 NN denotes Cell
T16400 33060-33067 NN denotes culture
T16401 33068-33071 CC denotes and
T16402 33072-33082 NN denotes generation
T16403 33083-33085 IN denotes of
T16404 33086-33092 JJ denotes stable
T16405 33098-33103 NNS denotes lines
T16406 33093-33097 NN denotes cell
T16407 33103-33104 . denotes .
T16408 33104-33356 sentence denotes MDCK cells (CCL-34; ATCC, Manassas, Virginia, United States) were cultured in DMEM (Sigma-Aldrich, St. Louis, Missouri, United States) supplemented with 10% FBS (Sigma-Aldrich), 100 U/ml of penicillin, and 100 μg/ml of streptomycin at 37 °C in 5% CO2.
T16409 33105-33109 NN denotes MDCK
T16410 33110-33115 NNS denotes cells
T16411 33171-33179 VBN denotes cultured
T16412 33116-33117 -LRB- denotes (
T16413 33125-33129 NN denotes ATCC
T16414 33117-33120 NN denotes CCL
T16415 33120-33121 HYPH denotes -
T16416 33121-33123 CD denotes 34
T16417 33123-33124 : denotes ;
T16418 33129-33131 , denotes ,
T16419 33131-33139 NNP denotes Manassas
T16420 33139-33141 , denotes ,
T16421 33141-33149 NNP denotes Virginia
T16422 33149-33151 , denotes ,
T16423 33151-33157 NNP denotes United
T16424 33158-33164 NNP denotes States
T16425 33164-33165 -RRB- denotes )
T16426 33166-33170 VBD denotes were
T16427 33180-33182 IN denotes in
T16428 33183-33187 NN denotes DMEM
T16429 33188-33189 -LRB- denotes (
T16430 33195-33202 NNP denotes Aldrich
T16431 33189-33194 NNP denotes Sigma
T16432 33194-33195 HYPH denotes -
T16433 33202-33204 , denotes ,
T16434 33204-33207 NNP denotes St.
T16435 33208-33213 NNP denotes Louis
T16436 33213-33215 , denotes ,
T16437 33215-33223 NNP denotes Missouri
T16438 33223-33225 , denotes ,
T16439 33225-33231 NNP denotes United
T16440 33232-33238 NNP denotes States
T16441 33238-33239 -RRB- denotes )
T16442 33240-33252 VBN denotes supplemented
T16443 33253-33257 IN denotes with
T16444 33258-33260 CD denotes 10
T16445 33260-33261 NN denotes %
T16446 33262-33265 NN denotes FBS
T16447 33266-33267 -LRB- denotes (
T16448 33273-33280 NNP denotes Aldrich
T16449 33267-33272 NNP denotes Sigma
T16450 33272-33273 HYPH denotes -
T16451 33280-33281 -RRB- denotes )
T16452 33281-33283 , denotes ,
T16453 33283-33286 CD denotes 100
T16454 33287-33288 NN denotes U
T16455 33288-33289 SYM denotes /
T16456 33289-33291 NN denotes ml
T16457 33292-33294 IN denotes of
T16458 33295-33305 NN denotes penicillin
T16459 33305-33307 , denotes ,
T16460 33307-33310 CC denotes and
T16461 33311-33314 CD denotes 100
T16462 33315-33317 NN denotes μg
T16463 33317-33318 SYM denotes /
T16464 33318-33320 NN denotes ml
T16465 33321-33323 IN denotes of
T16466 33324-33336 NN denotes streptomycin
T16467 33337-33339 IN denotes at
T16468 33340-33342 CD denotes 37
T16469 33343-33345 NN denotes °C
T16470 33346-33348 IN denotes in
T16471 33349-33350 CD denotes 5
T16472 33350-33351 NN denotes %
T16473 33352-33355 NN denotes CO2
T16474 33355-33356 . denotes .
T16475 33356-33596 sentence denotes To generate stable MDCK cell lines, cells were transfected using Lipofectamine 2000 (Invitrogen) and the pcDNA3.1 expression constructs (containing wild-type AQP2, AQP2-F204V, or no insert) and selected with 900 μg/ml G418 (Sigma-Aldrich).
T16476 33357-33359 TO denotes To
T16477 33360-33368 VB denotes generate
T16478 33404-33415 VBN denotes transfected
T16479 33369-33375 JJ denotes stable
T16480 33386-33391 NNS denotes lines
T16481 33376-33380 NN denotes MDCK
T16482 33381-33385 NN denotes cell
T16483 33391-33393 , denotes ,
T16484 33393-33398 NNS denotes cells
T16485 33399-33403 VBD denotes were
T16486 33416-33421 VBG denotes using
T16487 33422-33435 NN denotes Lipofectamine
T16488 33436-33440 CD denotes 2000
T16489 33441-33442 -LRB- denotes (
T16490 33442-33452 NNP denotes Invitrogen
T16491 33452-33453 -RRB- denotes )
T16492 33454-33457 CC denotes and
T16493 33458-33461 DT denotes the
T16494 33482-33492 NNS denotes constructs
T16495 33462-33470 NN denotes pcDNA3.1
T16496 33471-33481 NN denotes expression
T16497 33493-33494 -LRB- denotes (
T16498 33494-33504 VBG denotes containing
T16499 33505-33509 JJ denotes wild
T16500 33510-33514 NN denotes type
T16501 33509-33510 HYPH denotes -
T16502 33515-33519 NN denotes AQP2
T16503 33519-33521 , denotes ,
T16504 33521-33525 NN denotes AQP2
T16505 33526-33531 NN denotes F204V
T16506 33525-33526 HYPH denotes -
T16507 33531-33533 , denotes ,
T16508 33533-33535 CC denotes or
T16509 33536-33538 DT denotes no
T16510 33539-33545 NN denotes insert
T16511 33545-33546 -RRB- denotes )
T16512 33547-33550 CC denotes and
T16513 33551-33559 VBN denotes selected
T16514 33560-33564 IN denotes with
T16515 33565-33568 CD denotes 900
T16516 33569-33571 NN denotes μg
T16517 33575-33579 NN denotes G418
T16518 33571-33572 SYM denotes /
T16519 33572-33574 NN denotes ml
T16520 33580-33581 -LRB- denotes (
T16521 33587-33594 NNP denotes Aldrich
T16522 33581-33586 NNP denotes Sigma
T16523 33586-33587 HYPH denotes -
T16524 33594-33595 -RRB- denotes )
T16525 33595-33596 . denotes .
T16526 33596-33642 sentence denotes Individual colonies were expanded 14 d later.
T16527 33597-33607 JJ denotes Individual
T16528 33608-33616 NNS denotes colonies
T16529 33622-33630 VBN denotes expanded
T16530 33617-33621 VBD denotes were
T16531 33631-33633 CD denotes 14
T16532 33634-33635 NN denotes d
T16533 33636-33641 RB denotes later
T16534 33641-33642 . denotes .
T16535 33642-33732 sentence denotes For the duration of these experiments, the antibiotic was continually added to the media.
T16536 33643-33646 IN denotes For
T16537 33713-33718 VBN denotes added
T16538 33647-33650 DT denotes the
T16539 33651-33659 NN denotes duration
T16540 33660-33662 IN denotes of
T16541 33663-33668 DT denotes these
T16542 33669-33680 NNS denotes experiments
T16543 33680-33682 , denotes ,
T16544 33682-33685 DT denotes the
T16545 33686-33696 NN denotes antibiotic
T16546 33697-33700 VBD denotes was
T16547 33701-33712 RB denotes continually
T16548 33719-33721 IN denotes to
T16549 33722-33725 DT denotes the
T16550 33726-33731 NNS denotes media
T16551 33731-33732 . denotes .
T16552 33732-33843 sentence denotes Transient GFP transfections were carried out in subconfluent stable cells lines also using Lipofectamine 2000.
T16553 33733-33742 JJ denotes Transient
T16554 33747-33760 NNS denotes transfections
T16555 33743-33746 NN denotes GFP
T16556 33766-33773 VBN denotes carried
T16557 33761-33765 VBD denotes were
T16558 33774-33777 RP denotes out
T16559 33778-33780 IN denotes in
T16560 33781-33793 JJ denotes subconfluent
T16561 33807-33812 NNS denotes lines
T16562 33794-33800 JJ denotes stable
T16563 33801-33806 NNS denotes cells
T16564 33813-33817 RB denotes also
T16565 33818-33823 VBG denotes using
T16566 33824-33837 NN denotes Lipofectamine
T16567 33838-33842 CD denotes 2000
T16568 33842-33843 . denotes .
T16676 33845-33855 NN denotes Sequencing
T16677 33856-33858 IN denotes of
T16678 33859-33863 NN denotes Aqp2
T16679 33864-33867 CC denotes and
T16680 33868-33878 NN denotes genotyping
T16681 33879-33881 IN denotes of
T16682 33882-33886 NNS denotes mice
T16683 33886-33887 . denotes .
T16684 33887-33958 sentence denotes All exons of Aqp2 were amplified from mouse genomic DNA and sequenced.
T16685 33888-33891 DT denotes All
T16686 33892-33897 NNS denotes exons
T16687 33911-33920 VBN denotes amplified
T16688 33898-33900 IN denotes of
T16689 33901-33905 NN denotes Aqp2
T16690 33906-33910 VBD denotes were
T16691 33921-33925 IN denotes from
T16692 33926-33931 NN denotes mouse
T16693 33940-33943 NN denotes DNA
T16694 33932-33939 JJ denotes genomic
T16695 33944-33947 CC denotes and
T16696 33948-33957 VBN denotes sequenced
T16697 33957-33958 . denotes .
T16698 33958-34072 sentence denotes For genotyping, exon 4 was amplified using the primers 5′-TCAGAACTTGCCCACTAGCC-3′ and 5′-TGTAGAGGAGGGAACCGATG-3′.
T16699 33959-33962 IN denotes For
T16700 33986-33995 VBN denotes amplified
T16701 33963-33973 NN denotes genotyping
T16702 33973-33975 , denotes ,
T16703 33975-33979 NN denotes exon
T16704 33980-33981 CD denotes 4
T16705 33982-33985 VBD denotes was
T16706 33996-34001 VBG denotes using
T16707 34002-34005 DT denotes the
T16708 34006-34013 NNS denotes primers
T16709 34014-34015 CD denotes 5
T16710 34017-34037 NN denotes TCAGAACTTGCCCACTAGCC
T16711 34015-34016 SYM denotes ′
T16712 34016-34017 HYPH denotes -
T16713 34037-34038 HYPH denotes -
T16714 34038-34039 CD denotes 3
T16715 34039-34040 SYM denotes ′
T16716 34041-34044 CC denotes and
T16717 34045-34046 CD denotes 5
T16718 34048-34068 NN denotes TGTAGAGGAGGGAACCGATG
T16719 34046-34047 SYM denotes ′
T16720 34047-34048 HYPH denotes -
T16721 34068-34069 HYPH denotes -
T16722 34069-34070 CD denotes 3
T16723 34070-34071 SYM denotes ′
T16724 34071-34072 . denotes .
T16959 34074-34079 NN denotes Urine
T16960 34080-34092 NNS denotes measurements
T16961 34092-34093 . denotes .
T16962 34093-34277 sentence denotes Total urine output was measured by separately housing adult mice in Nalgene Metabolic Cages (Minimitter, Bend, Oregon, United States) for 2–3 d and collecting urine every 24 h period.
T16963 34094-34099 JJ denotes Total
T16964 34106-34112 NN denotes output
T16965 34100-34105 NN denotes urine
T16966 34117-34125 VBN denotes measured
T16967 34113-34116 VBD denotes was
T16968 34126-34128 IN denotes by
T16969 34129-34139 RB denotes separately
T16970 34140-34147 VBG denotes housing
T16971 34148-34153 JJ denotes adult
T16972 34154-34158 NNS denotes mice
T16973 34159-34161 IN denotes in
T16974 34162-34169 NNP denotes Nalgene
T16975 34180-34185 NNS denotes Cages
T16976 34170-34179 JJ denotes Metabolic
T16977 34186-34187 -LRB- denotes (
T16978 34187-34197 NNP denotes Minimitter
T16979 34197-34199 , denotes ,
T16980 34199-34203 NNP denotes Bend
T16981 34203-34205 , denotes ,
T16982 34205-34211 NNP denotes Oregon
T16983 34211-34213 , denotes ,
T16984 34213-34219 NNP denotes United
T16985 34220-34226 NNP denotes States
T16986 34226-34227 -RRB- denotes )
T16987 34228-34231 IN denotes for
T16988 34232-34233 CD denotes 2
T16989 34234-34235 CD denotes 3
T16990 34233-34234 SYM denotes –
T16991 34236-34237 NN denotes d
T16992 34238-34241 CC denotes and
T16993 34242-34252 VBG denotes collecting
T16994 34253-34258 NN denotes urine
T16995 34259-34264 DT denotes every
T16996 34270-34276 NN denotes period
T16997 34265-34267 CD denotes 24
T16998 34268-34269 NN denotes h
T16999 34276-34277 . denotes .
T17000 34277-34404 sentence denotes Urine osmolalities were determined using an Osmometer (Osmette 5004; Precision Systems, Natick, Massachusetts, United States).
T17001 34278-34283 NN denotes Urine
T17002 34284-34296 NNS denotes osmolalities
T17003 34302-34312 VBN denotes determined
T17004 34297-34301 VBD denotes were
T17005 34313-34318 VBG denotes using
T17006 34319-34321 DT denotes an
T17007 34322-34331 NN denotes Osmometer
T17008 34332-34333 -LRB- denotes (
T17009 34357-34364 NNP denotes Systems
T17010 34333-34340 NNP denotes Osmette
T17011 34341-34345 CD denotes 5004
T17012 34345-34346 : denotes ;
T17013 34347-34356 NNP denotes Precision
T17014 34364-34366 , denotes ,
T17015 34366-34372 NNP denotes Natick
T17016 34372-34374 , denotes ,
T17017 34374-34387 NNP denotes Massachusetts
T17018 34387-34389 , denotes ,
T17019 34389-34395 NNP denotes United
T17020 34396-34402 NNP denotes States
T17021 34402-34403 -RRB- denotes )
T17022 34403-34404 . denotes .
T17023 34404-34504 sentence denotes Urine concentrating experiments were carried out by intraperitoneal injection of dDAVP (0.4 μg/kg).
T17024 34405-34410 NN denotes Urine
T17025 34411-34424 VBG denotes concentrating
T17026 34425-34436 NNS denotes experiments
T17027 34442-34449 VBN denotes carried
T17028 34437-34441 VBD denotes were
T17029 34450-34453 RP denotes out
T17030 34454-34456 IN denotes by
T17031 34457-34472 JJ denotes intraperitoneal
T17032 34473-34482 NN denotes injection
T17033 34483-34485 IN denotes of
T17034 34486-34491 NN denotes dDAVP
T17035 34492-34493 -LRB- denotes (
T17036 34497-34499 NN denotes μg
T17037 34493-34496 CD denotes 0.4
T17038 34499-34500 SYM denotes /
T17039 34500-34502 NN denotes kg
T17040 34502-34503 -RRB- denotes )
T17041 34503-34504 . denotes .
T17042 34504-34577 sentence denotes Mice were injected twice with dDAVP, once at time 0 and again at 30 min.
T17043 34505-34509 NNS denotes Mice
T17044 34515-34523 VBN denotes injected
T17045 34510-34514 VBD denotes were
T17046 34524-34529 RB denotes twice
T17047 34530-34534 IN denotes with
T17048 34535-34540 NN denotes dDAVP
T17049 34540-34542 , denotes ,
T17050 34542-34546 RB denotes once
T17051 34547-34549 IN denotes at
T17052 34550-34554 NN denotes time
T17053 34555-34556 CD denotes 0
T17054 34557-34560 CC denotes and
T17055 34561-34566 RB denotes again
T17056 34567-34569 IN denotes at
T17057 34570-34572 CD denotes 30
T17058 34573-34576 NN denotes min
T17059 34576-34577 . denotes .
T17060 34577-34667 sentence denotes Urine was collected at the start of the experiment and 30 min after the second injection.
T17061 34578-34583 NN denotes Urine
T17062 34588-34597 VBN denotes collected
T17063 34584-34587 VBD denotes was
T17064 34598-34600 IN denotes at
T17065 34601-34604 DT denotes the
T17066 34605-34610 NN denotes start
T17068 34614-34617 DT denotes the
T17069 34618-34628 NN denotes experiment
T17070 34629-34632 CC denotes and
T17071 34633-34635 CD denotes 30
T17072 34636-34639 NN denotes min
T17073 34640-34645 IN denotes after
T17074 34646-34649 DT denotes the
T17075 34657-34666 NN denotes injection
T17076 34650-34656 JJ denotes second
T17077 34666-34667 . denotes .
T17265 34669-34675 NN denotes Kidney
T17266 34676-34684 NN denotes membrane
T17267 34685-34696 NN denotes preparation
T17268 34696-34697 . denotes .
T17269 34697-34878 sentence denotes Whole mouse kidneys were homogenized in 10 mM Tris (pH 7.4), 350 mM sucrose, and 5 mM EDTA containing protease inhibitors (Sigma-Aldrich, #P-8340) in a Potter-Elvehjem homogenizer.
T17270 34698-34703 JJ denotes Whole
T17271 34710-34717 NNS denotes kidneys
T17272 34704-34709 NN denotes mouse
T17273 34723-34734 VBN denotes homogenized
T17274 34718-34722 VBD denotes were
T17275 34735-34737 IN denotes in
T17276 34738-34740 CD denotes 10
T17277 34741-34743 NN denotes mM
T17278 34744-34748 NN denotes Tris
T17279 34749-34750 -LRB- denotes (
T17280 34750-34752 NN denotes pH
T17281 34753-34756 CD denotes 7.4
T17282 34756-34757 -RRB- denotes )
T17283 34757-34759 , denotes ,
T17284 34759-34762 CD denotes 350
T17285 34763-34765 NN denotes mM
T17286 34766-34773 NN denotes sucrose
T17287 34773-34775 , denotes ,
T17288 34775-34778 CC denotes and
T17289 34779-34780 CD denotes 5
T17290 34781-34783 NN denotes mM
T17291 34784-34788 NN denotes EDTA
T17292 34789-34799 VBG denotes containing
T17293 34800-34808 NN denotes protease
T17294 34809-34819 NNS denotes inhibitors
T17295 34820-34821 -LRB- denotes (
T17296 34837-34838 NN denotes P
T17297 34821-34826 NNP denotes Sigma
T17298 34827-34834 NNP denotes Aldrich
T17299 34826-34827 HYPH denotes -
T17300 34834-34836 , denotes ,
T17301 34836-34837 SYM denotes #
T17302 34838-34839 HYPH denotes -
T17303 34839-34843 CD denotes 8340
T17304 34843-34844 -RRB- denotes )
T17305 34845-34847 IN denotes in
T17306 34848-34849 DT denotes a
T17307 34866-34877 NN denotes homogenizer
T17308 34850-34856 NNP denotes Potter
T17309 34857-34865 NNP denotes Elvehjem
T17310 34856-34857 HYPH denotes -
T17311 34877-34878 . denotes .
T17312 34878-35018 sentence denotes The homogenate was centrifuged at 2,000 g for 10 min and the supernatant was subjected to ultracentrifugation at 100,000 g for 1 h at 4 °C.
T17313 34879-34882 DT denotes The
T17315 34898-34909 VBN denotes centrifuged
T17316 34894-34897 VBD denotes was
T17317 34910-34912 IN denotes at
T17318 34913-34918 CD denotes 2,000
T17319 34919-34920 NN denotes g
T17320 34921-34924 IN denotes for
T17321 34925-34927 CD denotes 10
T17322 34928-34931 NN denotes min
T17323 34932-34935 CC denotes and
T17324 34936-34939 DT denotes the
T17325 34940-34951 NN denotes supernatant
T17326 34956-34965 VBN denotes subjected
T17327 34952-34955 VBD denotes was
T17328 34966-34968 IN denotes to
T17329 34969-34988 NN denotes ultracentrifugation
T17330 34989-34991 IN denotes at
T17331 34992-34999 CD denotes 100,000
T17332 35000-35001 NN denotes g
T17333 35002-35005 IN denotes for
T17334 35006-35007 CD denotes 1
T17335 35008-35009 NN denotes h
T17336 35010-35012 IN denotes at
T17337 35013-35014 CD denotes 4
T17338 35015-35017 NN denotes °C
T17339 35017-35018 . denotes .
T17340 35018-35134 sentence denotes Pelleted membranes were resuspended in the same buffer, and protein concentration was determined by Bradford assay.
T17341 35019-35027 VBN denotes Pelleted
T17342 35028-35037 NNS denotes membranes
T17343 35043-35054 VBN denotes resuspended
T17344 35038-35042 VBD denotes were
T17345 35055-35057 IN denotes in
T17346 35058-35061 DT denotes the
T17347 35067-35073 NN denotes buffer
T17348 35062-35066 JJ denotes same
T17349 35073-35075 , denotes ,
T17350 35075-35078 CC denotes and
T17351 35079-35086 NN denotes protein
T17352 35087-35100 NN denotes concentration
T17353 35105-35115 VBN denotes determined
T17354 35101-35104 VBD denotes was
T17355 35116-35118 IN denotes by
T17356 35119-35127 NNP denotes Bradford
T17357 35128-35133 NN denotes assay
T17358 35133-35134 . denotes .
T17614 35136-35150 NN denotes Immunoblotting
T17615 35150-35151 . denotes .
T17616 35151-35277 sentence denotes Kidney membrane fractions (60 μg) were resolved on a 12% SDS-polyacrylamide gel and transferred to a nitrocellulose membrane.
T17617 35152-35158 NN denotes Kidney
T17618 35159-35167 NN denotes membrane
T17619 35168-35177 NNS denotes fractions
T17620 35191-35199 VBN denotes resolved
T17621 35178-35179 -LRB- denotes (
T17622 35182-35184 NN denotes μg
T17623 35179-35181 CD denotes 60
T17624 35184-35185 -RRB- denotes )
T17625 35186-35190 VBD denotes were
T17626 35200-35202 IN denotes on
T17627 35203-35204 DT denotes a
T17628 35228-35231 NN denotes gel
T17629 35205-35207 CD denotes 12
T17630 35207-35208 NN denotes %
T17631 35209-35212 NN denotes SDS
T17632 35213-35227 NN denotes polyacrylamide
T17633 35212-35213 HYPH denotes -
T17634 35232-35235 CC denotes and
T17635 35236-35247 VBN denotes transferred
T17636 35248-35250 IN denotes to
T17637 35251-35252 DT denotes a
T17638 35268-35276 NN denotes membrane
T17639 35253-35267 NN denotes nitrocellulose
T17640 35276-35277 . denotes .
T17641 35277-35523 sentence denotes Membranes were blocked in 5% nonfat milk in Tris-buffered saline with 0.05% Tween 20 (TBST), followed by an overnight incubation (at 4 °C) with AQP2 polyclonal antibody (Santa Cruz Biotechnology, Santa Cruz, California, United States; #sc-9882).
T17642 35278-35287 NNS denotes Membranes
T17643 35293-35300 VBN denotes blocked
T17644 35288-35292 VBD denotes were
T17645 35301-35303 IN denotes in
T17646 35304-35305 CD denotes 5
T17647 35305-35306 NN denotes %
T17648 35314-35318 NN denotes milk
T17649 35307-35313 JJ denotes nonfat
T17650 35319-35321 IN denotes in
T17651 35322-35326 NN denotes Tris
T17652 35327-35335 VBN denotes buffered
T17653 35326-35327 HYPH denotes -
T17654 35336-35342 NN denotes saline
T17655 35343-35347 IN denotes with
T17656 35348-35352 CD denotes 0.05
T17657 35352-35353 NN denotes %
T17658 35354-35359 NN denotes Tween
T17659 35360-35362 CD denotes 20
T17660 35363-35364 -LRB- denotes (
T17661 35364-35368 NN denotes TBST
T17662 35368-35369 -RRB- denotes )
T17663 35369-35371 , denotes ,
T17664 35371-35379 VBN denotes followed
T17665 35380-35382 IN denotes by
T17666 35383-35385 DT denotes an
T17667 35396-35406 NN denotes incubation
T17668 35386-35395 JJ denotes overnight
T17669 35407-35408 -LRB- denotes (
T17670 35408-35410 IN denotes at
T17671 35411-35412 CD denotes 4
T17672 35413-35415 NN denotes °C
T17673 35415-35416 -RRB- denotes )
T17674 35417-35421 IN denotes with
T17675 35422-35426 NN denotes AQP2
T17676 35438-35446 NN denotes antibody
T17677 35427-35437 JJ denotes polyclonal
T17678 35447-35448 -LRB- denotes (
T17679 35514-35516 NN denotes sc
T17680 35448-35453 NNP denotes Santa
T17681 35454-35458 NNP denotes Cruz
T17682 35459-35472 NNP denotes Biotechnology
T17683 35472-35474 , denotes ,
T17684 35474-35479 NNP denotes Santa
T17685 35480-35484 NNP denotes Cruz
T17686 35484-35486 , denotes ,
T17687 35486-35496 NNP denotes California
T17688 35496-35498 , denotes ,
T17689 35498-35504 NNP denotes United
T17690 35505-35511 NNP denotes States
T17691 35511-35512 : denotes ;
T17692 35513-35514 SYM denotes #
T17693 35516-35517 HYPH denotes -
T17694 35517-35521 CD denotes 9882
T17695 35521-35522 -RRB- denotes )
T17696 35522-35523 . denotes .
T17697 35523-35615 sentence denotes Membranes were washed in TBST then incubated with HRP-conjugated donkey anti-goat antibody.
T17698 35524-35533 NNS denotes Membranes
T17699 35539-35545 VBN denotes washed
T17700 35534-35538 VBD denotes were
T17701 35546-35548 IN denotes in
T17702 35549-35553 NN denotes TBST
T17703 35554-35558 RB denotes then
T17704 35559-35568 VBN denotes incubated
T17705 35569-35573 IN denotes with
T17706 35574-35577 NN denotes HRP
T17707 35578-35588 VBN denotes conjugated
T17708 35577-35578 HYPH denotes -
T17709 35606-35614 NN denotes antibody
T17710 35589-35595 NN denotes donkey
T17711 35596-35605 JJ denotes anti-goat
T17712 35614-35615 . denotes .
T17713 35615-35754 sentence denotes Membranes were washed further in TBST and bands were visualized using ECL reagent (Amersham Biosciences, Little Chalfont, United Kingdom).
T17714 35616-35625 NNS denotes Membranes
T17715 35631-35637 VBN denotes washed
T17716 35626-35630 VBD denotes were
T17717 35638-35645 RB denotes further
T17718 35646-35648 IN denotes in
T17719 35649-35653 NN denotes TBST
T17720 35654-35657 CC denotes and
T17721 35658-35663 NNS denotes bands
T17722 35669-35679 VBN denotes visualized
T17723 35664-35668 VBD denotes were
T17724 35680-35685 VBG denotes using
T17725 35686-35689 NN denotes ECL
T17726 35690-35697 NN denotes reagent
T17727 35698-35699 -LRB- denotes (
T17728 35708-35719 NNP denotes Biosciences
T17729 35699-35707 NNP denotes Amersham
T17730 35719-35721 , denotes ,
T17731 35721-35727 NNP denotes Little
T17732 35728-35736 NNP denotes Chalfont
T17733 35736-35738 , denotes ,
T17734 35738-35744 NNP denotes United
T17735 35745-35752 NNP denotes Kingdom
T17736 35752-35753 -RRB- denotes )
T17737 35753-35754 . denotes .
T17905 35756-35771 NN denotes Endoglycosidase
T17906 35772-35781 NN denotes digestion
T17907 35781-35782 . denotes .
T17908 35782-35938 sentence denotes Kidney membranes (60 μg) were incubated in 50 mM sodium phosphate (pH 5.5), 0.1% SDS, and 50 mM β-mercaptoethanol, heated to 100 °C for 5 min, then cooled.
T17909 35783-35789 NN denotes Kidney
T17910 35790-35799 NNS denotes membranes
T17911 35813-35822 VBN denotes incubated
T17912 35800-35801 -LRB- denotes (
T17913 35804-35806 NN denotes μg
T17914 35801-35803 CD denotes 60
T17915 35806-35807 -RRB- denotes )
T17916 35808-35812 VBD denotes were
T17917 35823-35825 IN denotes in
T17918 35826-35828 CD denotes 50
T17919 35829-35831 NN denotes mM
T17920 35839-35848 NN denotes phosphate
T17921 35832-35838 NN denotes sodium
T17922 35849-35850 -LRB- denotes (
T17923 35850-35852 NN denotes pH
T17924 35853-35856 CD denotes 5.5
T17925 35856-35857 -RRB- denotes )
T17926 35857-35859 , denotes ,
T17927 35859-35862 CD denotes 0.1
T17928 35862-35863 NN denotes %
T17929 35864-35867 NN denotes SDS
T17930 35867-35869 , denotes ,
T17931 35869-35872 CC denotes and
T17932 35873-35875 CD denotes 50
T17933 35876-35878 NN denotes mM
T17934 35881-35896 NN denotes mercaptoethanol
T17935 35879-35880 NN denotes β
T17936 35880-35881 HYPH denotes -
T17937 35896-35898 , denotes ,
T17938 35898-35904 VBN denotes heated
T17939 35905-35907 IN denotes to
T17940 35908-35911 CD denotes 100
T17941 35912-35914 NN denotes °C
T17942 35915-35918 IN denotes for
T17943 35919-35920 CD denotes 5
T17944 35921-35924 NN denotes min
T17945 35924-35926 , denotes ,
T17946 35926-35930 RB denotes then
T17947 35931-35937 VBN denotes cooled
T17948 35937-35938 . denotes .
T17949 35938-36026 sentence denotes Endoglycosidase H (0.01 units; Sigma-Aldrich) was added and incubated at 37 °C for 2 h.
T17950 35939-35954 NN denotes Endoglycosidase
T17951 35955-35956 NN denotes H
T17952 35989-35994 VBN denotes added
T17953 35957-35958 -LRB- denotes (
T17954 35976-35983 NNP denotes Aldrich
T17955 35958-35962 CD denotes 0.01
T17956 35963-35968 NNS denotes units
T17957 35968-35969 : denotes ;
T17958 35970-35975 NNP denotes Sigma
T17959 35975-35976 HYPH denotes -
T17960 35983-35984 -RRB- denotes )
T17961 35985-35988 VBD denotes was
T17962 35995-35998 CC denotes and
T17963 35999-36008 VBN denotes incubated
T17964 36009-36011 IN denotes at
T17965 36012-36014 CD denotes 37
T17966 36015-36017 NN denotes °C
T17967 36018-36021 IN denotes for
T17968 36022-36023 CD denotes 2
T17969 36024-36025 NN denotes h
T17970 36025-36026 . denotes .
T17971 36026-36093 sentence denotes The reaction was stopped by boiling the samples in Laemmli buffer.
T17972 36027-36030 DT denotes The
T17973 36031-36039 NN denotes reaction
T17974 36044-36051 VBN denotes stopped
T17975 36040-36043 VBD denotes was
T17976 36052-36054 IN denotes by
T17977 36055-36062 VBG denotes boiling
T17978 36063-36066 DT denotes the
T17979 36067-36074 NNS denotes samples
T17980 36075-36077 IN denotes in
T17981 36078-36085 NNP denotes Laemmli
T17982 36086-36092 NN denotes buffer
T17983 36092-36093 . denotes .
T17984 36093-36148 sentence denotes Total reactants were immunoblotted as described above.
T17985 36094-36099 JJ denotes Total
T17986 36100-36109 NNS denotes reactants
T17987 36115-36128 VBN denotes immunoblotted
T17988 36110-36114 VBD denotes were
T17989 36129-36131 IN denotes as
T17990 36132-36141 VBN denotes described
T17991 36142-36147 RB denotes above
T17992 36147-36148 . denotes .
T18783 36150-36171 NN denotes Coimmunoprecipitation
T18784 36172-36175 CC denotes and
T18785 36176-36189 NN denotes biotinylation
T18786 36190-36192 IN denotes in
T18787 36193-36197 NN denotes MDCK
T18788 36198-36203 NNS denotes cells
T18789 36203-36204 . denotes .
T18790 36204-36351 sentence denotes MDCK cells stably expressing wild-type AQP2 (grown on 10-cm plates) were transfected with pEGFP-wild-type AQP2, pEGFP-AQP2-F204V, or vector alone.
T18791 36205-36209 NN denotes MDCK
T18792 36210-36215 NNS denotes cells
T18793 36278-36289 VBN denotes transfected
T18794 36216-36222 RB denotes stably
T18795 36223-36233 VBG denotes expressing
T18796 36234-36238 JJ denotes wild
T18797 36239-36243 NN denotes type
T18798 36238-36239 HYPH denotes -
T18799 36244-36248 NN denotes AQP2
T18800 36249-36250 -LRB- denotes (
T18801 36250-36255 VBN denotes grown
T18802 36256-36258 IN denotes on
T18803 36259-36261 CD denotes 10
T18804 36262-36264 NN denotes cm
T18805 36261-36262 HYPH denotes -
T18806 36265-36271 NNS denotes plates
T18807 36271-36272 -RRB- denotes )
T18808 36273-36277 VBD denotes were
T18809 36290-36294 IN denotes with
T18810 36295-36300 NN denotes pEGFP
T18811 36306-36310 NN denotes type
T18812 36300-36301 HYPH denotes -
T18813 36301-36305 JJ denotes wild
T18814 36305-36306 HYPH denotes -
T18815 36311-36315 NN denotes AQP2
T18816 36315-36317 , denotes ,
T18817 36317-36322 NN denotes pEGFP
T18818 36328-36333 NN denotes F204V
T18819 36322-36323 HYPH denotes -
T18820 36323-36327 NN denotes AQP2
T18821 36327-36328 HYPH denotes -
T18822 36333-36335 , denotes ,
T18823 36335-36337 CC denotes or
T18824 36338-36344 NN denotes vector
T18825 36345-36350 RB denotes alone
T18826 36350-36351 . denotes .
T18827 36351-36444 sentence denotes The cells were homogenized in 10 mM Tris (pH 7.4), 1 mM EDTA, and 250 mM sucrose 40 h later.
T18828 36352-36355 DT denotes The
T18829 36356-36361 NNS denotes cells
T18830 36367-36378 VBN denotes homogenized
T18831 36362-36366 VBD denotes were
T18832 36379-36381 IN denotes in
T18833 36382-36384 CD denotes 10
T18834 36385-36387 NN denotes mM
T18835 36388-36392 NN denotes Tris
T18836 36393-36394 -LRB- denotes (
T18837 36394-36396 NN denotes pH
T18838 36397-36400 CD denotes 7.4
T18839 36400-36401 -RRB- denotes )
T18840 36401-36403 , denotes ,
T18841 36403-36404 CD denotes 1
T18842 36405-36407 NN denotes mM
T18843 36408-36412 NN denotes EDTA
T18844 36412-36414 , denotes ,
T18845 36414-36417 CC denotes and
T18846 36418-36421 CD denotes 250
T18847 36422-36424 NN denotes mM
T18848 36425-36432 NN denotes sucrose
T18849 36433-36435 CD denotes 40
T18850 36436-36437 NN denotes h
T18851 36438-36443 RB denotes later
T18852 36443-36444 . denotes .
T18853 36444-36511 sentence denotes The clarified supernatant was centrifuged at 200,000 g for 30 min.
T18854 36445-36448 DT denotes The
T18855 36459-36470 NN denotes supernatant
T18856 36449-36458 VBN denotes clarified
T18857 36475-36486 VBN denotes centrifuged
T18858 36471-36474 VBD denotes was
T18859 36487-36489 IN denotes at
T18860 36490-36497 CD denotes 200,000
T18861 36498-36499 NN denotes g
T18862 36500-36503 IN denotes for
T18863 36504-36506 CD denotes 30
T18864 36507-36510 NN denotes min
T18865 36510-36511 . denotes .
T18866 36511-36636 sentence denotes Pelleted membranes were resuspended in the same buffer but containing 4% sodium deoxycholate and incubated at 37 °C for 1 h.
T18867 36512-36520 VBN denotes Pelleted
T18868 36521-36530 NNS denotes membranes
T18869 36609-36618 VBN denotes incubated
T18870 36531-36535 VBD denotes were
T18871 36536-36547 VBN denotes resuspended
T18872 36548-36550 IN denotes in
T18873 36551-36554 DT denotes the
T18874 36560-36566 NN denotes buffer
T18875 36555-36559 JJ denotes same
T18876 36567-36570 IN denotes but
T18877 36571-36581 VBG denotes containing
T18878 36582-36583 CD denotes 4
T18879 36583-36584 NN denotes %
T18880 36592-36604 NN denotes deoxycholate
T18881 36585-36591 NN denotes sodium
T18882 36605-36608 CC denotes and
T18883 36619-36621 IN denotes at
T18884 36622-36624 CD denotes 37
T18885 36625-36627 NN denotes °C
T18886 36628-36631 IN denotes for
T18887 36632-36633 CD denotes 1
T18888 36634-36635 NN denotes h
T18889 36635-36636 . denotes .
T18890 36636-36734 sentence denotes From the dissolved membranes, a 30 μl sample was removed and used as the total membrane fraction.
T18891 36637-36641 IN denotes From
T18892 36686-36693 VBN denotes removed
T18893 36642-36645 DT denotes the
T18894 36656-36665 NNS denotes membranes
T18895 36646-36655 VBN denotes dissolved
T18896 36665-36667 , denotes ,
T18897 36667-36668 DT denotes a
T18898 36675-36681 NN denotes sample
T18899 36669-36671 CD denotes 30
T18900 36672-36674 NN denotes μl
T18901 36682-36685 VBD denotes was
T18902 36694-36697 CC denotes and
T18903 36698-36702 VBN denotes used
T18904 36703-36705 IN denotes as
T18905 36706-36709 DT denotes the
T18906 36725-36733 NN denotes fraction
T18907 36710-36715 JJ denotes total
T18908 36716-36724 NN denotes membrane
T18909 36733-36734 . denotes .
T18910 36734-36903 sentence denotes The remaining membranes were diluted with 600 μl of the homogenization buffer, and incubated with 1 μl of GFP antisera (Invitrogen, #46–0092) and protein A/G sepharose.
T18911 36735-36738 DT denotes The
T18912 36749-36758 NNS denotes membranes
T18913 36739-36748 VBG denotes remaining
T18914 36764-36771 VBN denotes diluted
T18915 36759-36763 VBD denotes were
T18916 36772-36776 IN denotes with
T18917 36777-36780 CD denotes 600
T18918 36781-36783 NN denotes μl
T18919 36784-36786 IN denotes of
T18920 36787-36790 DT denotes the
T18921 36806-36812 NN denotes buffer
T18922 36791-36805 NN denotes homogenization
T18923 36812-36814 , denotes ,
T18924 36814-36817 CC denotes and
T18925 36818-36827 VBN denotes incubated
T18926 36828-36832 IN denotes with
T18927 36833-36834 CD denotes 1
T18928 36835-36837 NN denotes μl
T18929 36838-36840 IN denotes of
T18930 36841-36844 NN denotes GFP
T18931 36845-36853 NNS denotes antisera
T18932 36854-36855 -LRB- denotes (
T18933 36871-36875 CD denotes 0092
T18934 36855-36865 NNP denotes Invitrogen
T18935 36865-36867 , denotes ,
T18936 36867-36868 SYM denotes #
T18937 36868-36870 CD denotes 46
T18938 36870-36871 HYPH denotes –
T18939 36875-36876 -RRB- denotes )
T18940 36877-36880 CC denotes and
T18941 36881-36888 NN denotes protein
T18942 36893-36902 NN denotes sepharose
T18943 36889-36890 NN denotes A
T18944 36891-36892 NN denotes G
T18945 36890-36891 HYPH denotes /
T18946 36902-36903 . denotes .
T18947 36903-37035 sentence denotes Following overnight incubation, the precipitated proteins were washed in RIPA buffer and finally boiled in 50 μl of Laemmli buffer.
T18948 36904-36913 VBG denotes Following
T18949 36967-36973 VBN denotes washed
T18950 36914-36923 JJ denotes overnight
T18951 36924-36934 NN denotes incubation
T18952 36934-36936 , denotes ,
T18953 36936-36939 DT denotes the
T18954 36953-36961 NN denotes proteins
T18955 36940-36952 VBN denotes precipitated
T18956 36962-36966 VBD denotes were
T18957 36974-36976 IN denotes in
T18958 36977-36981 NN denotes RIPA
T18959 36982-36988 NN denotes buffer
T18960 36989-36992 CC denotes and
T18961 36993-37000 RB denotes finally
T18962 37001-37007 VBN denotes boiled
T18963 37008-37010 IN denotes in
T18964 37011-37013 CD denotes 50
T18965 37014-37016 NN denotes μl
T18966 37017-37019 IN denotes of
T18967 37020-37027 NNP denotes Laemmli
T18968 37028-37034 NN denotes buffer
T18969 37034-37035 . denotes .
T18970 37035-37118 sentence denotes Half of the total membrane and the IP fractions were processed for immunoblotting.
T18971 37036-37040 NN denotes Half
T18972 37089-37098 VBN denotes processed
T18973 37041-37043 IN denotes of
T18974 37044-37047 DT denotes the
T18975 37054-37062 NN denotes membrane
T18976 37048-37053 JJ denotes total
T18977 37063-37066 CC denotes and
T18978 37067-37070 DT denotes the
T18979 37074-37083 NNS denotes fractions
T18980 37071-37073 NN denotes IP
T18981 37084-37088 VBD denotes were
T18982 37099-37102 IN denotes for
T18983 37103-37117 NN denotes immunoblotting
T18984 37117-37118 . denotes .
T18985 37118-37180 sentence denotes Cell surface biotinylation was performed in a similar manner.
T18986 37119-37123 NN denotes Cell
T18987 37132-37145 NN denotes biotinylation
T18988 37124-37131 NN denotes surface
T18989 37150-37159 VBN denotes performed
T18990 37146-37149 VBD denotes was
T18991 37160-37162 IN denotes in
T18992 37163-37164 DT denotes a
T18993 37173-37179 NN denotes manner
T18994 37165-37172 JJ denotes similar
T18995 37179-37180 . denotes .
T18996 37180-37313 sentence denotes However, pEGFP-AQP2-F204V, was transfected into MDCK cells stably expressing wild-type AQP2 and cells made stable with vector alone.
T18997 37181-37188 RB denotes However
T18998 37212-37223 VBN denotes transfected
T18999 37188-37190 , denotes ,
T19000 37190-37195 NN denotes pEGFP
T19001 37201-37206 NN denotes F204V
T19002 37195-37196 HYPH denotes -
T19003 37196-37200 NN denotes AQP2
T19004 37200-37201 HYPH denotes -
T19005 37206-37208 , denotes ,
T19006 37208-37211 VBD denotes was
T19007 37224-37228 IN denotes into
T19008 37229-37233 NN denotes MDCK
T19009 37234-37239 NNS denotes cells
T19010 37240-37246 RB denotes stably
T19011 37247-37257 VBG denotes expressing
T19012 37258-37262 JJ denotes wild
T19013 37263-37267 NN denotes type
T19014 37262-37263 HYPH denotes -
T19015 37268-37272 NN denotes AQP2
T19016 37273-37276 CC denotes and
T19017 37277-37282 NNS denotes cells
T19018 37283-37287 VBN denotes made
T19019 37288-37294 JJ denotes stable
T19020 37295-37299 IN denotes with
T19021 37300-37306 NN denotes vector
T19022 37307-37312 RB denotes alone
T19023 37312-37313 . denotes .
T19024 37313-37550 sentence denotes Twenty-four hours post-transfection, cells were stimulated with forskolin, trypsinized, resuspended in 1 ml of PBS (2.5 × 106 cells/ml), and incubated with 0.5 mg of NHS-PEO4-biotin (Pierce Biotechnology) for 30 min at room temperature.
T19025 37314-37320 CD denotes Twenty
T19026 37321-37325 CD denotes four
T19027 37320-37321 HYPH denotes -
T19028 37326-37331 NNS denotes hours
T19029 37332-37349 RB denotes post-transfection
T19030 37362-37372 VBN denotes stimulated
T19031 37349-37351 , denotes ,
T19032 37351-37356 NNS denotes cells
T19033 37357-37361 VBD denotes were
T19034 37373-37377 IN denotes with
T19035 37378-37387 NN denotes forskolin
T19036 37387-37389 , denotes ,
T19037 37389-37400 VBN denotes trypsinized
T19038 37400-37402 , denotes ,
T19039 37402-37413 VBN denotes resuspended
T19040 37414-37416 IN denotes in
T19041 37417-37418 CD denotes 1
T19042 37419-37421 NN denotes ml
T19043 37422-37424 IN denotes of
T19044 37425-37428 NN denotes PBS
T19045 37429-37430 -LRB- denotes (
T19046 37440-37445 NNS denotes cells
T19047 37430-37433 CD denotes 2.5
T19048 37436-37439 CD denotes 106
T19049 37434-37435 SYM denotes ×
T19050 37445-37446 SYM denotes /
T19051 37446-37448 NNS denotes ml
T19052 37448-37449 -RRB- denotes )
T19053 37449-37451 , denotes ,
T19054 37451-37454 CC denotes and
T19055 37455-37464 VBN denotes incubated
T19056 37465-37469 IN denotes with
T19057 37470-37473 CD denotes 0.5
T19058 37474-37476 NN denotes mg
T19059 37477-37479 IN denotes of
T19060 37480-37483 NN denotes NHS
T19061 37489-37495 NN denotes biotin
T19062 37483-37484 HYPH denotes -
T19063 37484-37488 NN denotes PEO4
T19064 37488-37489 HYPH denotes -
T19065 37496-37497 -LRB- denotes (
T19066 37504-37517 NNP denotes Biotechnology
T19067 37497-37503 NNP denotes Pierce
T19068 37517-37518 -RRB- denotes )
T19069 37519-37522 IN denotes for
T19070 37523-37525 CD denotes 30
T19071 37526-37529 NN denotes min
T19072 37530-37532 IN denotes at
T19073 37533-37537 NN denotes room
T19074 37538-37549 NN denotes temperature
T19075 37549-37550 . denotes .
T19076 37550-37690 sentence denotes Cells were washed once in 10 mM Tris (pH 8) and three times in PBS, after which membranes were purified and solubilized as described above.
T19077 37551-37556 NNS denotes Cells
T19078 37562-37568 VBN denotes washed
T19079 37557-37561 VBD denotes were
T19080 37569-37573 RB denotes once
T19081 37574-37576 IN denotes in
T19082 37577-37579 CD denotes 10
T19083 37580-37582 NN denotes mM
T19084 37583-37587 NN denotes Tris
T19085 37588-37589 -LRB- denotes (
T19086 37589-37591 NN denotes pH
T19087 37592-37593 CD denotes 8
T19088 37593-37594 -RRB- denotes )
T19089 37595-37598 CC denotes and
T19090 37599-37604 CD denotes three
T19091 37605-37610 NNS denotes times
T19092 37611-37613 IN denotes in
T19093 37614-37617 NN denotes PBS
T19094 37617-37619 , denotes ,
T19095 37619-37624 IN denotes after
T19096 37646-37654 VBN denotes purified
T19097 37625-37630 WDT denotes which
T19098 37631-37640 NNS denotes membranes
T19099 37641-37645 VBD denotes were
T19100 37655-37658 CC denotes and
T19101 37659-37670 VBN denotes solubilized
T19102 37671-37673 IN denotes as
T19103 37674-37683 VBN denotes described
T19104 37684-37689 RB denotes above
T19105 37689-37690 . denotes .
T19106 37690-37806 sentence denotes Solubilized membranes were incubated with 20 μl of immobilized streptavidin (Pierce Biotechnology) for 2 h at 4 °C.
T19107 37691-37702 VBN denotes Solubilized
T19108 37703-37712 NNS denotes membranes
T19109 37718-37727 VBN denotes incubated
T19110 37713-37717 VBD denotes were
T19111 37728-37732 IN denotes with
T19112 37733-37735 CD denotes 20
T19113 37736-37738 NN denotes μl
T19114 37739-37741 IN denotes of
T19115 37742-37753 VBN denotes immobilized
T19116 37754-37766 NN denotes streptavidin
T19117 37767-37768 -LRB- denotes (
T19118 37775-37788 NNP denotes Biotechnology
T19119 37768-37774 NNP denotes Pierce
T19120 37788-37789 -RRB- denotes )
T19121 37790-37793 IN denotes for
T19122 37794-37795 CD denotes 2
T19123 37796-37797 NN denotes h
T19124 37798-37800 IN denotes at
T19125 37801-37802 CD denotes 4
T19126 37803-37805 NN denotes °C
T19127 37805-37806 . denotes .
T19128 37806-37906 sentence denotes Finally the precipitated proteins were washed in RIPA buffer and boiled in 50 μl of Laemmli buffer.
T19129 37807-37814 RB denotes Finally
T19130 37846-37852 VBN denotes washed
T19131 37815-37818 DT denotes the
T19132 37832-37840 NN denotes proteins
T19133 37819-37831 VBN denotes precipitated
T19134 37841-37845 VBD denotes were
T19135 37853-37855 IN denotes in
T19136 37856-37860 NN denotes RIPA
T19137 37861-37867 NN denotes buffer
T19138 37868-37871 CC denotes and
T19139 37872-37878 VBN denotes boiled
T19140 37879-37881 IN denotes in
T19141 37882-37884 CD denotes 50
T19142 37885-37887 NN denotes μl
T19143 37888-37890 IN denotes of
T19144 37891-37898 NNP denotes Laemmli
T19145 37899-37905 NN denotes buffer
T19146 37905-37906 . denotes .
T19147 37906-37999 sentence denotes Total cells and the biotinylated precipitates were immunoblotting using an antibody to AQP2.
T19148 37907-37912 JJ denotes Total
T19149 37913-37918 NNS denotes cells
T19150 37958-37972 VBG denotes immunoblotting
T19151 37919-37922 CC denotes and
T19152 37923-37926 DT denotes the
T19153 37940-37952 NNS denotes precipitates
T19154 37927-37939 VBN denotes biotinylated
T19155 37953-37957 VBD denotes were
T19156 37973-37978 VBG denotes using
T19157 37979-37981 DT denotes an
T19158 37982-37990 NN denotes antibody
T19159 37991-37993 IN denotes to
T19160 37994-37998 NN denotes AQP2
T19161 37998-37999 . denotes .
T19603 38001-38007 NN denotes Kidney
T19604 38008-38028 NN denotes immunohistochemistry
T19605 38028-38029 . denotes .
T19606 38029-38105 sentence denotes Whole mouse kidneys were fixed in 10% phosphate-buffered formalin for 24 h.
T19607 38030-38035 JJ denotes Whole
T19608 38042-38049 NNS denotes kidneys
T19609 38036-38041 NN denotes mouse
T19610 38055-38060 VBN denotes fixed
T19611 38050-38054 VBD denotes were
T19612 38061-38063 IN denotes in
T19613 38064-38066 CD denotes 10
T19614 38066-38067 NN denotes %
T19615 38087-38095 NN denotes formalin
T19616 38068-38077 NN denotes phosphate
T19617 38078-38086 VBN denotes buffered
T19618 38077-38078 HYPH denotes -
T19619 38096-38099 IN denotes for
T19620 38100-38102 CD denotes 24
T19621 38103-38104 NN denotes h
T19622 38104-38105 . denotes .
T19623 38105-38173 sentence denotes Kidneys were embedded in paraffin, and 5-μm sections were prepared.
T19624 38106-38113 NNS denotes Kidneys
T19625 38119-38127 VBN denotes embedded
T19626 38114-38118 VBD denotes were
T19627 38128-38130 IN denotes in
T19628 38131-38139 NN denotes paraffin
T19629 38139-38141 , denotes ,
T19630 38141-38144 CC denotes and
T19631 38145-38146 CD denotes 5
T19632 38147-38149 NN denotes μm
T19633 38146-38147 HYPH denotes -
T19634 38150-38158 NNS denotes sections
T19635 38164-38172 VBN denotes prepared
T19636 38159-38163 VBD denotes were
T19637 38172-38173 . denotes .
T19638 38173-38325 sentence denotes Following antigen retrieval using 10 mM sodium citrate (pH 8) for 10 min at 98 °C, sections were sequentially probed, first for AQP3 and then for AQP2.
T19639 38174-38183 VBG denotes Following
T19640 38284-38290 VBN denotes probed
T19641 38184-38191 NN denotes antigen
T19642 38192-38201 NN denotes retrieval
T19643 38202-38207 VBG denotes using
T19644 38208-38210 CD denotes 10
T19645 38211-38213 NN denotes mM
T19646 38221-38228 NN denotes citrate
T19647 38214-38220 NN denotes sodium
T19648 38229-38230 -LRB- denotes (
T19649 38230-38232 NN denotes pH
T19650 38233-38234 CD denotes 8
T19651 38234-38235 -RRB- denotes )
T19652 38236-38239 IN denotes for
T19653 38240-38242 CD denotes 10
T19654 38243-38246 NN denotes min
T19655 38247-38249 IN denotes at
T19656 38250-38252 CD denotes 98
T19657 38253-38255 NN denotes °C
T19658 38255-38257 , denotes ,
T19659 38257-38265 NNS denotes sections
T19660 38266-38270 VBD denotes were
T19661 38271-38283 RB denotes sequentially
T19662 38290-38292 , denotes ,
T19663 38292-38297 RB denotes first
T19664 38298-38301 IN denotes for
T19665 38302-38306 NN denotes AQP3
T19666 38307-38310 CC denotes and
T19667 38311-38315 RB denotes then
T19668 38316-38319 IN denotes for
T19669 38320-38324 NN denotes AQP2
T19670 38324-38325 . denotes .
T19671 38325-38449 sentence denotes Sections were incubated in 5% donkey serum and then in goat anti-AQP3 antibody (1:100; Santa Cruz Biotechnology; #sc-9885).
T19672 38326-38334 NNS denotes Sections
T19673 38340-38349 VBN denotes incubated
T19674 38335-38339 VBD denotes were
T19675 38350-38352 IN denotes in
T19676 38353-38354 CD denotes 5
T19677 38354-38355 NN denotes %
T19678 38363-38368 NN denotes serum
T19679 38356-38362 NN denotes donkey
T19680 38369-38372 CC denotes and
T19681 38373-38377 RB denotes then
T19682 38378-38380 IN denotes in
T19683 38381-38385 NN denotes goat
T19684 38396-38404 NN denotes antibody
T19685 38386-38395 JJ denotes anti-AQP3
T19686 38405-38406 -LRB- denotes (
T19687 38440-38442 NN denotes sc
T19688 38406-38407 CD denotes 1
T19689 38407-38408 SYM denotes :
T19690 38408-38411 CD denotes 100
T19691 38411-38412 : denotes ;
T19692 38413-38418 NNP denotes Santa
T19693 38419-38423 NNP denotes Cruz
T19694 38424-38437 NNP denotes Biotechnology
T19695 38437-38438 : denotes ;
T19696 38439-38440 SYM denotes #
T19697 38442-38443 HYPH denotes -
T19698 38443-38447 CD denotes 9885
T19699 38447-38448 -RRB- denotes )
T19700 38448-38449 . denotes .
T19701 38449-38599 sentence denotes Slides were washed with PBS and incubated with AlexaFluor 488-conjugated donkey anti-goat antibody (Molecular Probes, Eugene, Oregon, United States).
T19702 38450-38456 NNS denotes Slides
T19703 38462-38468 VBN denotes washed
T19704 38457-38461 VBD denotes were
T19705 38469-38473 IN denotes with
T19706 38474-38477 NN denotes PBS
T19707 38478-38481 CC denotes and
T19708 38482-38491 VBN denotes incubated
T19709 38492-38496 IN denotes with
T19710 38497-38507 NNP denotes AlexaFluor
T19711 38512-38522 VBN denotes conjugated
T19712 38508-38511 CD denotes 488
T19713 38511-38512 HYPH denotes -
T19714 38540-38548 NN denotes antibody
T19715 38523-38529 NN denotes donkey
T19716 38530-38539 JJ denotes anti-goat
T19717 38549-38550 -LRB- denotes (
T19718 38560-38566 NNP denotes Probes
T19719 38550-38559 NNP denotes Molecular
T19720 38566-38568 , denotes ,
T19721 38568-38574 NNP denotes Eugene
T19722 38574-38576 , denotes ,
T19723 38576-38582 NNP denotes Oregon
T19724 38582-38584 , denotes ,
T19725 38584-38590 NNP denotes United
T19726 38591-38597 NNP denotes States
T19727 38597-38598 -RRB- denotes )
T19728 38598-38599 . denotes .
T19729 38599-38865 sentence denotes The slides were subsequently blocked in 5% chicken serum, incubated with a rabbit anti-AQP2 antibody (1:250; USB, Cleveland, Ohio, United States; #A3000–06), which was detected with a AlexaFluor 594-conjugated chicken anti-rabbit antibody (1:500; Molecular Probes).
T19730 38600-38603 DT denotes The
T19731 38604-38610 NNS denotes slides
T19732 38658-38667 VBN denotes incubated
T19733 38611-38615 VBD denotes were
T19734 38616-38628 RB denotes subsequently
T19735 38629-38636 VBN denotes blocked
T19736 38637-38639 IN denotes in
T19737 38640-38641 CD denotes 5
T19738 38641-38642 NN denotes %
T19739 38651-38656 NN denotes serum
T19740 38643-38650 NN denotes chicken
T19741 38656-38658 , denotes ,
T19742 38668-38672 IN denotes with
T19743 38673-38674 DT denotes a
T19744 38692-38700 NN denotes antibody
T19745 38675-38681 NN denotes rabbit
T19746 38682-38691 JJ denotes anti-AQP2
T19747 38701-38702 -LRB- denotes (
T19748 38747-38752 NN denotes A3000
T19749 38702-38703 CD denotes 1
T19750 38703-38704 SYM denotes :
T19751 38704-38707 CD denotes 250
T19752 38707-38708 : denotes ;
T19753 38709-38712 NNP denotes USB
T19754 38712-38714 , denotes ,
T19755 38714-38723 NNP denotes Cleveland
T19756 38723-38725 , denotes ,
T19757 38725-38729 NNP denotes Ohio
T19758 38729-38731 , denotes ,
T19759 38731-38737 NNP denotes United
T19760 38738-38744 NNP denotes States
T19761 38744-38745 : denotes ;
T19762 38746-38747 SYM denotes #
T19763 38752-38753 HYPH denotes –
T19764 38753-38755 CD denotes 06
T19765 38755-38756 -RRB- denotes )
T19766 38756-38758 , denotes ,
T19767 38758-38763 WDT denotes which
T19768 38768-38776 VBN denotes detected
T19769 38764-38767 VBD denotes was
T19770 38777-38781 IN denotes with
T19771 38782-38783 DT denotes a
T19772 38830-38838 NN denotes antibody
T19773 38784-38794 NNP denotes AlexaFluor
T19774 38799-38809 VBN denotes conjugated
T19775 38795-38798 CD denotes 594
T19776 38798-38799 HYPH denotes -
T19777 38810-38817 NN denotes chicken
T19778 38818-38829 JJ denotes anti-rabbit
T19779 38839-38840 -LRB- denotes (
T19780 38857-38863 NNP denotes Probes
T19781 38840-38841 CD denotes 1
T19782 38841-38842 SYM denotes :
T19783 38842-38845 CD denotes 500
T19784 38845-38846 : denotes ;
T19785 38847-38856 NNP denotes Molecular
T19786 38863-38864 -RRB- denotes )
T19787 38864-38865 . denotes .
T19788 38865-38982 sentence denotes The sections were stained with DAPI and mounted in Vectashield (Vector Labs, Burlingame, California, United States).
T19789 38866-38869 DT denotes The
T19790 38870-38878 NNS denotes sections
T19791 38884-38891 VBN denotes stained
T19792 38879-38883 VBD denotes were
T19793 38892-38896 IN denotes with
T19794 38897-38901 NN denotes DAPI
T19795 38902-38905 CC denotes and
T19796 38906-38913 VBD denotes mounted
T19797 38914-38916 IN denotes in
T19798 38917-38928 NNP denotes Vectashield
T19799 38929-38930 -LRB- denotes (
T19800 38937-38941 NNP denotes Labs
T19801 38930-38936 NNP denotes Vector
T19802 38941-38943 , denotes ,
T19803 38943-38953 NNP denotes Burlingame
T19804 38953-38955 , denotes ,
T19805 38955-38965 NNP denotes California
T19806 38965-38967 , denotes ,
T19807 38967-38973 NNP denotes United
T19808 38974-38980 NNP denotes States
T19809 38980-38981 -RRB- denotes )
T19810 38981-38982 . denotes .
T20424 38984-39003 NN denotes Immunocytochemistry
T20425 39004-39006 IN denotes on
T20426 39007-39011 NN denotes MDCK
T20427 39012-39017 NNS denotes cells
T20428 39017-39018 . denotes .
T20429 39018-39229 sentence denotes MDCK stable cell lines expressing vector alone, wild-type AQP2, or AQP2-F204V (and in some cases transiently expressing a GFP construct) were grown on fibronectin-coated coverslips until tight junctions formed.
T20430 39019-39023 NN denotes MDCK
T20431 39036-39041 NNS denotes lines
T20432 39024-39030 JJ denotes stable
T20433 39031-39035 NN denotes cell
T20434 39161-39166 VBN denotes grown
T20435 39042-39052 VBG denotes expressing
T20436 39053-39059 NN denotes vector
T20437 39060-39065 JJ denotes alone
T20438 39065-39067 , denotes ,
T20439 39067-39071 JJ denotes wild
T20440 39072-39076 NN denotes type
T20441 39071-39072 HYPH denotes -
T20442 39077-39081 NN denotes AQP2
T20443 39081-39083 , denotes ,
T20444 39083-39085 CC denotes or
T20445 39086-39090 NN denotes AQP2
T20446 39091-39096 NN denotes F204V
T20447 39090-39091 HYPH denotes -
T20448 39097-39098 -LRB- denotes (
T20449 39098-39101 CC denotes and
T20450 39102-39104 IN denotes in
T20451 39128-39138 VBG denotes expressing
T20452 39105-39109 DT denotes some
T20453 39110-39115 NNS denotes cases
T20454 39116-39127 RB denotes transiently
T20455 39139-39140 DT denotes a
T20456 39145-39154 NN denotes construct
T20457 39141-39144 NN denotes GFP
T20458 39154-39155 -RRB- denotes )
T20459 39156-39160 VBD denotes were
T20460 39167-39169 IN denotes on
T20461 39170-39181 NN denotes fibronectin
T20462 39182-39188 VBN denotes coated
T20463 39181-39182 HYPH denotes -
T20464 39189-39199 NNS denotes coverslips
T20465 39200-39205 IN denotes until
T20466 39222-39228 VBD denotes formed
T20467 39206-39211 JJ denotes tight
T20468 39212-39221 NNS denotes junctions
T20469 39228-39229 . denotes .
T20470 39229-39326 sentence denotes Cells were treated with or without 150 μM forskolin for 90 min, and fixed in methanol at −20 °C.
T20471 39230-39235 NNS denotes Cells
T20472 39241-39248 VBN denotes treated
T20473 39236-39240 VBD denotes were
T20474 39249-39253 IN denotes with
T20475 39254-39256 CC denotes or
T20476 39257-39264 IN denotes without
T20477 39265-39268 CD denotes 150
T20478 39269-39271 NN denotes μM
T20479 39272-39281 NN denotes forskolin
T20480 39282-39285 IN denotes for
T20481 39286-39288 CD denotes 90
T20482 39289-39292 NN denotes min
T20483 39292-39294 , denotes ,
T20484 39294-39297 CC denotes and
T20485 39298-39303 VBN denotes fixed
T20486 39304-39306 IN denotes in
T20487 39307-39315 NN denotes methanol
T20488 39316-39318 IN denotes at
T20489 39319-39320 SYM denotes −
T20490 39320-39322 CD denotes 20
T20491 39323-39325 NN denotes °C
T20492 39325-39326 . denotes .
T20493 39326-39492 sentence denotes Subsequently, cells were washed and permeabilized in 0.2% Triton X-100 for 5 min, and sequentially probed for AQP2 and organelle markers for either the PM or the ER.
T20494 39327-39339 RB denotes Subsequently
T20495 39352-39358 VBN denotes washed
T20496 39339-39341 , denotes ,
T20497 39341-39346 NNS denotes cells
T20498 39347-39351 VBD denotes were
T20499 39359-39362 CC denotes and
T20500 39363-39376 VBN denotes permeabilized
T20501 39377-39379 IN denotes in
T20502 39380-39383 CD denotes 0.2
T20503 39383-39384 NN denotes %
T20504 39392-39393 NN denotes X
T20505 39385-39391 NN denotes Triton
T20506 39393-39394 HYPH denotes -
T20507 39394-39397 CD denotes 100
T20508 39398-39401 IN denotes for
T20509 39402-39403 CD denotes 5
T20510 39404-39407 NN denotes min
T20511 39407-39409 , denotes ,
T20512 39409-39412 CC denotes and
T20513 39413-39425 RB denotes sequentially
T20514 39426-39432 VBN denotes probed
T20515 39433-39436 IN denotes for
T20516 39437-39441 NN denotes AQP2
T20517 39442-39445 CC denotes and
T20518 39446-39455 NN denotes organelle
T20519 39456-39463 NNS denotes markers
T20520 39464-39467 IN denotes for
T20521 39468-39474 CC denotes either
T20522 39479-39481 NN denotes PM
T20523 39475-39478 DT denotes the
T20524 39482-39484 CC denotes or
T20525 39485-39488 DT denotes the
T20526 39489-39491 NN denotes ER
T20527 39491-39492 . denotes .
T20528 39492-39662 sentence denotes AQP2 was detected using goat anti-AQP2 (1:100; Santa Cruz Biotechnology; #sc-9882) and a 1:200 dilution of AlexaFluor 488-conjugated donkey anti-goat secondary antibody.
T20529 39493-39497 NN denotes AQP2
T20530 39502-39510 VBN denotes detected
T20531 39498-39501 VBD denotes was
T20532 39511-39516 VBG denotes using
T20533 39517-39521 NN denotes goat
T20534 39522-39531 JJ denotes anti-AQP2
T20535 39532-39533 -LRB- denotes (
T20536 39567-39569 NN denotes sc
T20537 39533-39534 CD denotes 1
T20538 39534-39535 SYM denotes :
T20539 39535-39538 CD denotes 100
T20540 39538-39539 : denotes ;
T20541 39540-39545 NNP denotes Santa
T20542 39546-39550 NNP denotes Cruz
T20543 39551-39564 NNP denotes Biotechnology
T20544 39564-39565 : denotes ;
T20545 39566-39567 SYM denotes #
T20546 39569-39570 HYPH denotes -
T20547 39570-39574 CD denotes 9882
T20548 39574-39575 -RRB- denotes )
T20549 39576-39579 CC denotes and
T20550 39580-39581 DT denotes a
T20551 39588-39596 NN denotes dilution
T20552 39582-39583 CD denotes 1
T20553 39584-39587 CD denotes 200
T20554 39583-39584 SYM denotes :
T20555 39597-39599 IN denotes of
T20556 39600-39610 NNP denotes AlexaFluor
T20557 39615-39625 VBN denotes conjugated
T20558 39611-39614 CD denotes 488
T20559 39614-39615 HYPH denotes -
T20560 39653-39661 NN denotes antibody
T20561 39626-39632 NN denotes donkey
T20562 39633-39642 JJ denotes anti-goat
T20563 39643-39652 JJ denotes secondary
T20564 39661-39662 . denotes .
T20565 39662-40070 sentence denotes The PM and ER were probed using mouse anti-Na+/K+-ATPase (Upstate, Waltham, Massachusetts, United States) or rabbit anti-calnexin (Stressgen Biotechnology, Victoria, British Columbia, Canada) antibodies and the secondary antibodies, Cy3-conjugated goat anti-mouse (1:200; Jackson ImmunoResearch, West Grove, Pennsylvania, United States) or AlexaFluor 594-conjugated chicken anti-rabbit (1:200) respectively.
T20566 39663-39666 DT denotes The
T20567 39667-39669 NN denotes PM
T20568 39682-39688 VBN denotes probed
T20569 39670-39673 CC denotes and
T20570 39674-39676 NN denotes ER
T20571 39677-39681 VBD denotes were
T20572 39689-39694 VBG denotes using
T20573 39695-39700 NN denotes mouse
T20574 39713-39719 NN denotes ATPase
T20575 39701-39709 JJ denotes anti-Na+
T20576 39709-39710 HYPH denotes /
T20577 39710-39712 NN denotes K+
T20578 39712-39713 HYPH denotes -
T20579 39855-39865 NNS denotes antibodies
T20580 39720-39721 -LRB- denotes (
T20581 39721-39728 NNP denotes Upstate
T20582 39728-39730 , denotes ,
T20583 39730-39737 NNP denotes Waltham
T20584 39737-39739 , denotes ,
T20585 39739-39752 NNP denotes Massachusetts
T20586 39752-39754 , denotes ,
T20587 39754-39760 NNP denotes United
T20588 39761-39767 NNP denotes States
T20589 39767-39768 -RRB- denotes )
T20590 39769-39771 CC denotes or
T20591 39772-39778 NN denotes rabbit
T20592 39779-39792 JJ denotes anti-calnexin
T20593 39793-39794 -LRB- denotes (
T20594 39804-39817 NNP denotes Biotechnology
T20595 39794-39803 NNP denotes Stressgen
T20596 39817-39819 , denotes ,
T20597 39819-39827 NNP denotes Victoria
T20598 39827-39829 , denotes ,
T20599 39829-39836 NNP denotes British
T20600 39837-39845 NNP denotes Columbia
T20601 39845-39847 , denotes ,
T20602 39847-39853 NNP denotes Canada
T20603 39853-39854 -RRB- denotes )
T20604 39866-39869 CC denotes and
T20605 39870-39873 DT denotes the
T20606 39884-39894 NNS denotes antibodies
T20607 39874-39883 JJ denotes secondary
T20608 39894-39896 , denotes ,
T20609 39896-39899 NN denotes Cy3
T20610 39900-39910 VBN denotes conjugated
T20611 39899-39900 HYPH denotes -
T20612 39911-39915 NN denotes goat
T20613 39916-39926 JJ denotes anti-mouse
T20614 39927-39928 -LRB- denotes (
T20615 39943-39957 NNP denotes ImmunoResearch
T20616 39928-39929 CD denotes 1
T20617 39929-39930 SYM denotes :
T20618 39930-39933 CD denotes 200
T20619 39933-39934 : denotes ;
T20620 39935-39942 NNP denotes Jackson
T20621 39957-39959 , denotes ,
T20622 39959-39963 NNP denotes West
T20623 39964-39969 NNP denotes Grove
T20624 39969-39971 , denotes ,
T20625 39971-39983 NNP denotes Pennsylvania
T20626 39983-39985 , denotes ,
T20627 39985-39991 NNP denotes United
T20628 39992-39998 NNP denotes States
T20629 39998-39999 -RRB- denotes )
T20630 40000-40002 CC denotes or
T20631 40003-40013 NNP denotes AlexaFluor
T20632 40018-40028 VBN denotes conjugated
T20633 40014-40017 CD denotes 594
T20634 40017-40018 HYPH denotes -
T20635 40029-40036 NN denotes chicken
T20636 40037-40048 JJ denotes anti-rabbit
T20637 40049-40050 -LRB- denotes (
T20638 40050-40051 CD denotes 1
T20639 40051-40052 SYM denotes :
T20640 40052-40055 CD denotes 200
T20641 40055-40056 -RRB- denotes )
T20642 40057-40069 RB denotes respectively
T20643 40069-40070 . denotes .
T20644 40070-40150 sentence denotes Cells were washed in PBS, counterstained with DAPI, and mounted in Vectashield.
T20645 40071-40076 NNS denotes Cells
T20646 40082-40088 VBN denotes washed
T20647 40077-40081 VBD denotes were
T20648 40089-40091 IN denotes in
T20649 40092-40095 NN denotes PBS
T20650 40095-40097 , denotes ,
T20651 40097-40111 VBN denotes counterstained
T20652 40112-40116 IN denotes with
T20653 40117-40121 NN denotes DAPI
T20654 40121-40123 , denotes ,
T20655 40123-40126 CC denotes and
T20656 40127-40134 VBN denotes mounted
T20657 40135-40137 IN denotes in
T20658 40138-40149 NNP denotes Vectashield
T20659 40149-40150 . denotes .
T20660 40150-40368 sentence denotes In experiments in which GFP fusions were used, AQP2 was probed using the antibody combination used for kidney immunohistochemistry in order to detect the AQP2 at 594 nm, to distinguish between the GFP fusion proteins.
T20661 40151-40153 IN denotes In
T20662 40207-40213 VBN denotes probed
T20663 40154-40165 NNS denotes experiments
T20664 40166-40168 IN denotes in
T20665 40192-40196 VBN denotes used
T20666 40169-40174 WDT denotes which
T20667 40175-40178 NN denotes GFP
T20668 40179-40186 NNS denotes fusions
T20669 40187-40191 VBD denotes were
T20670 40196-40198 , denotes ,
T20671 40198-40202 NN denotes AQP2
T20672 40203-40206 VBD denotes was
T20673 40214-40219 VBG denotes using
T20674 40220-40223 DT denotes the
T20675 40233-40244 NN denotes combination
T20676 40224-40232 NN denotes antibody
T20677 40245-40249 VBN denotes used
T20678 40250-40253 IN denotes for
T20679 40254-40260 NN denotes kidney
T20680 40261-40281 NN denotes immunohistochemistry
T20681 40282-40284 IN denotes in
T20682 40285-40290 NN denotes order
T20683 40291-40293 TO denotes to
T20684 40294-40300 VB denotes detect
T20685 40301-40304 DT denotes the
T20686 40305-40309 NN denotes AQP2
T20687 40310-40312 IN denotes at
T20688 40313-40316 CD denotes 594
T20689 40317-40319 NN denotes nm
T20690 40319-40321 , denotes ,
T20691 40321-40323 TO denotes to
T20692 40324-40335 VB denotes distinguish
T20693 40336-40343 IN denotes between
T20694 40344-40347 DT denotes the
T20695 40359-40367 NN denotes proteins
T20696 40348-40351 NN denotes GFP
T20697 40352-40358 NN denotes fusion
T20698 40367-40368 . denotes .
T20832 40370-40378 JJ denotes Confocal
T20833 40379-40389 NN denotes microscopy
T20834 40389-40390 . denotes .
T20835 40390-40538 sentence denotes Optical z-section images were collected on a BioRad (Hercules, California, United States) Rainbow Radiance 2100 Laser Scanning Confocal Microscope.
T20836 40391-40398 JJ denotes Optical
T20837 40409-40415 NNS denotes images
T20838 40399-40400 NN denotes z
T20839 40401-40408 NN denotes section
T20840 40400-40401 HYPH denotes -
T20841 40421-40430 VBN denotes collected
T20842 40416-40420 VBD denotes were
T20843 40431-40433 IN denotes on
T20844 40434-40435 DT denotes a
T20845 40527-40537 NN denotes Microscope
T20846 40436-40442 NNP denotes BioRad
T20847 40443-40444 -LRB- denotes (
T20848 40444-40452 NNP denotes Hercules
T20849 40452-40454 , denotes ,
T20850 40454-40464 NNP denotes California
T20851 40464-40466 , denotes ,
T20852 40466-40472 NNP denotes United
T20853 40473-40479 NNP denotes States
T20854 40479-40480 -RRB- denotes )
T20855 40481-40488 NNP denotes Rainbow
T20856 40489-40497 NNP denotes Radiance
T20857 40498-40502 CD denotes 2100
T20858 40503-40508 NN denotes Laser
T20859 40509-40517 NN denotes Scanning
T20860 40518-40526 NN denotes Confocal
T20861 40537-40538 . denotes .
T20862 40538-40723 sentence denotes Image stacks were flattened, or sectioned along the z-axis, then further processed using BioRad Laser Sharp 2000 software and Image J software (v. 1.32; National Institutes of Health).
T20863 40539-40544 NN denotes Image
T20864 40545-40551 NNS denotes stacks
T20865 40557-40566 VBN denotes flattened
T20866 40552-40556 VBD denotes were
T20867 40566-40568 , denotes ,
T20868 40568-40570 CC denotes or
T20869 40571-40580 VBN denotes sectioned
T20870 40581-40586 IN denotes along
T20871 40587-40590 DT denotes the
T20872 40593-40597 NN denotes axis
T20873 40591-40592 NN denotes z
T20874 40592-40593 HYPH denotes -
T20875 40597-40599 , denotes ,
T20876 40599-40603 RB denotes then
T20877 40612-40621 VBN denotes processed
T20878 40604-40611 RB denotes further
T20879 40622-40627 VBG denotes using
T20880 40628-40634 NNP denotes BioRad
T20881 40652-40660 NN denotes software
T20882 40635-40640 NNP denotes Laser
T20883 40641-40646 NNP denotes Sharp
T20884 40647-40651 CD denotes 2000
T20885 40661-40664 CC denotes and
T20886 40665-40670 NNP denotes Image
T20887 40671-40672 NNP denotes J
T20888 40673-40681 NN denotes software
T20889 40682-40683 -LRB- denotes (
T20890 40701-40711 NNPS denotes Institutes
T20891 40683-40685 NN denotes v.
T20892 40686-40690 CD denotes 1.32
T20893 40690-40691 : denotes ;
T20894 40692-40700 NNP denotes National
T20895 40712-40714 IN denotes of
T20896 40715-40721 NNP denotes Health
T20897 40721-40722 -RRB- denotes )
T20898 40722-40723 . denotes .
T20899 40723-40803 sentence denotes Colocalization was performed using the overlay coefficient of Image J software.
T20900 40724-40738 NN denotes Colocalization
T20901 40743-40752 VBN denotes performed
T20902 40739-40742 VBD denotes was
T20903 40753-40758 VBG denotes using
T20904 40759-40762 DT denotes the
T20905 40771-40782 NN denotes coefficient
T20906 40763-40770 JJ denotes overlay
T20907 40783-40785 IN denotes of
T20908 40786-40791 NNP denotes Image
T20909 40792-40793 NNP denotes J
T20910 40794-40802 NN denotes software
T20911 40802-40803 . denotes .
T17067 34611-34613 IN denotes of
R12 T609 T612 nsubj insipidus,is
R13 T610 T609 amod nephrogenic,insipidus
R14 T611 T609 compound diabetes,insipidus
R38 T638 T636 pobj insipidus,with
R39 T639 T638 compound diabetes,insipidus
R47 T647 T645 pobj gene,in
R48 T648 T649 nmod vasopressin,receptor
R49 T649 T647 nmod receptor,gene
R50 T650 T649 nummod 2,receptor
R51 T651 T649 punct (,receptor
R52 T652 T649 appos Avpr2,receptor
R53 T653 T647 punct ),gene
R56 T656 T647 conj gene,gene
R57 T657 T658 npadvmod vasopressin,sensitive
R59 T659 T658 punct -,sensitive
R60 T660 T661 nmod water,channel
R61 T661 T656 nmod channel,gene
R62 T662 T656 nmod aquaporin,gene
R63 T663 T662 punct -,aquaporin
R64 T664 T662 nummod 2,aquaporin
R65 T665 T662 punct (,aquaporin
R66 T666 T662 appos Aqp2,aquaporin
R67 T667 T656 punct ),gene
R81 T683 T681 pobj model,of
R82 T684 T683 amod viable,model
R83 T685 T683 compound animal,model
R87 T691 T688 pobj screening,Through
R88 T692 T691 amod genetic,screening
R91 T695 T697 amod mutagenized,mice
R92 T696 T695 punct -,mutagenized
R102 T706 T704 pobj model,of
R103 T707 T706 compound mouse,model
R109 T713 T711 pobj mutation,with
R110 T714 T713 compound F204V,mutation
R113 T717 T715 pobj gene,in
R114 T718 T717 compound Aqp2,gene
R119 T725 T721 pobj models,Unlike
R120 T726 T725 amod murine,models
R133 T739 T737 dobj disorder,recapitulate
R134 T740 T739 amod human,disorder
R137 T744 T747 nsubj experiments,suggest
R138 T745 T746 advmod in,vitro
R139 T746 T744 amod vitro,experiments
R142 T750 T748 dobj lines,using
R143 T751 T750 compound cell,lines
R145 T753 T755 nsubj mutations,result
R146 T754 T753 compound Aqp2,mutations
R153 T761 T759 pobj pore,of
R154 T762 T761 amod mutant,pore
R155 T763 T761 compound water,pore
R168 T778 T776 pobj translocation,of
R169 T779 T778 compound AQP2,translocation
R172 T782 T780 pobj defect,as
R173 T783 T782 amod molecular,defect
R176 T786 T784 pobj insipidus,in
R177 T787 T786 compound diabetes,insipidus
R180 T790 T788 pobj organism,in
R181 T791 T790 amod intact,organism
R187 T799 T797 dobj line,using
R188 T800 T799 amod renal,line
R189 T801 T799 compound cell,line
R192 T804 T795 advcl retained,show
R193 T805 T806 det the,protein
R195 T807 T806 amod mutated,protein
R196 T808 T806 punct ", ",protein
R197 T809 T810 compound AQP2,F204V
R199 T811 T810 punct -,F204V
R200 T812 T804 punct ", ",retained
R201 T813 T804 auxpass is,retained
R204 T816 T814 pobj reticulum,in
R205 T817 T816 amod endoplasmic,reticulum
R208 T820 T804 conj rescued,retained
R209 T821 T822 det this,localization
R211 T823 T822 amod abnormal,localization
R212 T824 T820 aux can,rescued
R213 T825 T820 auxpass be,rescued
R216 T828 T830 compound type,protein
R217 T829 T828 punct -,type
R221 T834 T837 nsubj model,allows
R222 T835 T834 amod novel,model
R223 T836 T834 compound mouse,model
R226 T840 T838 pobj studies,for
R227 T841 T840 amod mechanistic,studies
R229 T843 T840 cc as,studies
R230 T844 T843 advmod well,as
R234 T848 T846 pobj therapies,of
R235 T849 T847 cc and,pharmacological
R236 T850 T847 conj gene,pharmacological
R254 T2464 T2465 amod Nephrogenic,insipidus
R255 T2465 T2467 nsubj insipidus,is
R256 T2466 T2465 compound diabetes,insipidus
R257 T2468 T2465 punct (,insipidus
R258 T2469 T2465 appos NDI,insipidus
R259 T2470 T2467 punct ),is
R260 T2471 T2472 det a,disease
R261 T2472 T2467 attr disease,is
R262 T2473 T2472 acl characterized,disease
R263 T2474 T2473 agent by,characterized
R264 T2475 T2476 amod excessive,urination
R265 T2476 T2474 pobj urination,by
R266 T2477 T2476 cc and,urination
R267 T2478 T2476 conj thirst,urination
R268 T2479 T2473 punct ", ",characterized
R269 T2480 T2473 prep despite,characterized
R270 T2481 T2482 amod normal,production
R271 T2482 T2480 pobj production,despite
R272 T2483 T2482 prep of,production
R273 T2484 T2485 det the,hormone
R274 T2485 T2483 pobj hormone,of
R275 T2486 T2485 amod antidiuretic,hormone
R276 T2487 T2488 compound arginine,vasopressin
R277 T2488 T2485 appos vasopressin,hormone
R278 T2489 T2488 punct (,vasopressin
R279 T2490 T2488 appos AVP,vasopressin
R280 T2491 T2467 punct ),is
R281 T2492 T2493 punct [,1
R282 T2493 T2467 parataxis 1,is
R283 T2494 T2493 punct ],1
R284 T2495 T2467 punct .,is
R285 T2497 T2498 det The,forms
R286 T2498 T2500 nsubj forms,are
R287 T2499 T2498 amod inherited,forms
R288 T2501 T2502 advmod either,linked
R289 T2502 T2500 acomp linked,are
R290 T2503 T2502 npadvmod X,linked
R291 T2504 T2502 punct -,linked
R292 T2505 T2502 prep as,linked
R293 T2506 T2507 det a,consequence
R294 T2507 T2505 pobj consequence,as
R295 T2508 T2507 prep of,consequence
R296 T2509 T2508 pobj mutation,of
R297 T2510 T2509 prep of,mutation
R298 T2511 T2512 det the,gene
R299 T2512 T2510 pobj gene,of
R300 T2513 T2512 compound Avpr2,gene
R301 T2514 T2515 punct [,2
R302 T2515 T2502 parataxis 2,linked
R303 T2516 T2515 punct ],2
R304 T2517 T2502 punct ", ",linked
R305 T2518 T2502 cc or,linked
R306 T2519 T2502 conj autosomal,linked
R307 T2520 T2521 amod due,to
R308 T2521 T2519 prep to,autosomal
R309 T2522 T2521 pobj mutation,to
R310 T2523 T2522 prep of,mutation
R311 T2524 T2525 det the,gene
R312 T2525 T2523 pobj gene,of
R313 T2526 T2525 compound Aqp2,gene
R314 T2527 T2528 punct [,3
R315 T2528 T2519 parataxis 3,autosomal
R316 T2529 T2528 punct ],3
R317 T2530 T2500 punct .,are
R318 T2532 T2533 nsubj Aquaporin,is
R319 T2534 T2532 punct -,Aquaporin
R320 T2535 T2532 nummod 2,Aquaporin
R321 T2536 T2532 punct (,Aquaporin
R322 T2537 T2532 appos AQP2,Aquaporin
R323 T2538 T2533 punct ),is
R324 T2539 T2540 det a,protein
R325 T2540 T2533 attr protein,is
R326 T2541 T2542 npadvmod pore,forming
R327 T2542 T2540 amod forming,protein
R328 T2543 T2542 punct -,forming
R329 T2544 T2540 acl belonging,protein
R330 T2545 T2544 prep to,belonging
R331 T2546 T2547 det a,family
R332 T2547 T2545 pobj family,to
R333 T2548 T2547 prep of,family
R334 T2549 T2550 compound water,channels
R335 T2550 T2548 pobj channels,of
R336 T2551 T2552 punct [,4
R337 T2552 T2533 parataxis 4,is
R338 T2553 T2552 punct ],4
R339 T2554 T2533 punct ", ",is
R340 T2555 T2533 cc and,is
R341 T2556 T2557 nsubjpass it,expressed
R342 T2557 T2533 conj expressed,is
R343 T2558 T2557 auxpass is,expressed
R344 T2559 T2557 prep in,expressed
R345 T2560 T2561 amod collecting,duct
R346 T2561 T2563 nmod duct,cells
R347 T2562 T2561 punct -,duct
R348 T2563 T2559 pobj cells,in
R349 T2564 T2563 amod principal,cells
R350 T2565 T2557 prep in,expressed
R351 T2566 T2567 det the,kidney
R352 T2567 T2565 pobj kidney,in
R353 T2568 T2569 punct [,5
R354 T2569 T2557 parataxis 5,expressed
R355 T2570 T2569 punct ],5
R356 T2571 T2557 punct .,expressed
R357 T2573 T2574 advmod Generally,permit
R358 T2575 T2576 det these,proteins
R359 T2576 T2574 nsubj proteins,permit
R360 T2577 T2578 det the,passage
R361 T2578 T2574 dobj passage,permit
R362 T2579 T2578 prep of,passage
R363 T2580 T2579 pobj water,of
R364 T2581 T2578 prep through,passage
R365 T2582 T2583 det the,membrane
R366 T2583 T2581 pobj membrane,through
R367 T2584 T2583 compound plasma,membrane
R368 T2585 T2583 punct (,membrane
R369 T2586 T2583 appos PM,membrane
R370 T2587 T2583 punct ),membrane
R371 T2588 T2583 prep of,membrane
R372 T2589 T2588 pobj cells,of
R373 T2590 T2589 punct ", ",cells
R374 T2591 T2592 dep several,carry
R375 T2592 T2589 relcl carry,cells
R376 T2593 T2591 prep of,several
R377 T2594 T2593 pobj which,of
R378 T2595 T2592 prt out,carry
R379 T2596 T2597 det this,role
R380 T2597 T2592 dobj role,carry
R381 T2598 T2599 advmod specifically,in
R382 T2599 T2592 prep in,carry
R383 T2600 T2601 det the,process
R384 T2601 T2599 pobj process,in
R385 T2602 T2601 prep of,process
R386 T2603 T2604 compound water,reabsorption
R387 T2604 T2602 pobj reabsorption,of
R388 T2605 T2604 prep from,reabsorption
R389 T2606 T2605 pobj urine,from
R390 T2607 T2604 prep in,reabsorption
R391 T2608 T2609 det the,kidney
R392 T2609 T2607 pobj kidney,in
R393 T2610 T2574 punct .,permit
R394 T2612 T2613 nsubjpass It,established
R395 T2614 T2613 aux has,established
R396 T2615 T2613 auxpass been,established
R397 T2616 T2617 mark that,tetramerize
R398 T2617 T2613 ccomp tetramerize,established
R399 T2618 T2617 nsubj aquaporins,tetramerize
R400 T2619 T2617 punct ", ",tetramerize
R401 T2620 T2621 mark although,functional
R402 T2621 T2617 advcl functional,tetramerize
R403 T2622 T2621 prep as,functional
R404 T2623 T2624 det a,monomer
R405 T2624 T2622 pobj monomer,as
R406 T2625 T2617 punct ", ",tetramerize
R407 T2626 T2617 prep before,tetramerize
R408 T2627 T2628 poss their,insertion
R409 T2628 T2626 pobj insertion,before
R410 T2629 T2628 prep into,insertion
R411 T2630 T2631 det the,membrane
R412 T2631 T2629 pobj membrane,into
R413 T2632 T2631 compound plasma,membrane
R414 T2633 T2634 punct [,6
R415 T2634 T2613 parataxis 6,established
R416 T2635 T2634 nummod 4,6
R417 T2636 T2634 punct ",",6
R418 T2637 T2634 punct ],6
R419 T2638 T2613 punct .,established
R420 T2640 T2641 advmod Furthermore,targeted
R421 T2641 T2648 ccomp targeted,routed
R422 T2642 T2643 det these,proteins
R423 T2643 T2641 nsubjpass proteins,targeted
R424 T2644 T2641 aux can,targeted
R425 T2645 T2641 advmod also,targeted
R426 T2646 T2641 auxpass be,targeted
R427 T2647 T2641 advmod differentially,targeted
R428 T2649 T2641 prep to,targeted
R429 T2650 T2651 amod distinct,regions
R430 T2651 T2649 pobj regions,to
R431 T2652 T2651 prep of,regions
R432 T2653 T2654 det the,PM
R433 T2654 T2652 pobj PM,of
R434 T2655 T2648 punct ;,routed
R435 T2656 T2648 prep for,routed
R436 T2657 T2656 pobj example,for
R437 T2658 T2648 punct ", ",routed
R438 T2659 T2648 nsubjpass AQP2,routed
R439 T2660 T2648 auxpass is,routed
R440 T2661 T2648 prep to,routed
R441 T2662 T2663 det the,membrane
R442 T2663 T2661 pobj membrane,to
R443 T2664 T2663 amod apical,membrane
R444 T2665 T2663 prep of,membrane
R445 T2666 T2665 pobj cells,of
R446 T2667 T2666 acl surrounding,cells
R447 T2668 T2669 det the,duct
R448 T2669 T2667 dobj duct,surrounding
R449 T2670 T2669 amod collecting,duct
R450 T2671 T2648 punct ", ",routed
R451 T2672 T2673 mark whereas,inserted
R452 T2673 T2648 advcl inserted,routed
R453 T2674 T2675 amod other,aquaporins
R454 T2675 T2673 nsubjpass aquaporins,inserted
R455 T2676 T2675 punct (,aquaporins
R456 T2677 T2675 appos AQP3,aquaporins
R457 T2678 T2677 cc or,AQP3
R458 T2679 T2677 conj 4,AQP3
R459 T2680 T2673 punct ),inserted
R460 T2681 T2673 auxpass are,inserted
R461 T2682 T2673 prep into,inserted
R462 T2683 T2684 det the,face
R463 T2684 T2682 pobj face,into
R464 T2685 T2684 amod basolateral,face
R465 T2686 T2648 punct .,routed
R466 T2688 T2689 prep Unlike,inserted
R467 T2690 T2691 det all,members
R468 T2691 T2688 pobj members,Unlike
R469 T2692 T2691 amod other,members
R470 T2693 T2691 compound family,members
R471 T2694 T2689 punct ", ",inserted
R472 T2695 T2689 nsubjpass AQP2,inserted
R473 T2696 T2689 auxpass is,inserted
R474 T2697 T2689 neg not,inserted
R475 T2698 T2689 advmod constitutively,inserted
R476 T2699 T2689 prep into,inserted
R477 T2700 T2701 det the,membrane
R478 T2701 T2699 pobj membrane,into
R479 T2702 T2701 compound plasma,membrane
R480 T2703 T2689 punct .,inserted
R481 T2705 T2706 prep Under,resides
R482 T2706 T2712 ccomp resides,translocates
R483 T2707 T2708 amod basal,conditions
R484 T2708 T2705 pobj conditions,Under
R485 T2709 T2706 punct ", ",resides
R486 T2710 T2711 det the,protein
R487 T2711 T2706 nsubj protein,resides
R488 T2713 T2706 prep in,resides
R489 T2714 T2715 amod subapical,vesicles
R490 T2715 T2713 pobj vesicles,in
R491 T2716 T2715 amod intracellular,vesicles
R492 T2717 T2712 punct ;,translocates
R493 T2718 T2712 advmod however,translocates
R494 T2719 T2712 punct ", ",translocates
R495 T2720 T2712 prep under,translocates
R496 T2721 T2720 pobj conditions,under
R497 T2722 T2721 acl requiring,conditions
R498 T2723 T2724 compound water,retention
R499 T2724 T2722 dobj retention,requiring
R500 T2725 T2712 nsubj AQP2,translocates
R501 T2726 T2712 prep to,translocates
R502 T2727 T2728 det the,membrane
R503 T2728 T2726 pobj membrane,to
R504 T2729 T2728 amod apical,membrane
R505 T2730 T2712 punct ", ",translocates
R506 T2731 T2712 advcl permitting,translocates
R507 T2732 T2733 compound water,reabsorption
R508 T2733 T2731 dobj reabsorption,permitting
R509 T2734 T2735 punct [,8
R510 T2735 T2712 parataxis 8,translocates
R511 T2736 T2735 nummod 7,8
R512 T2737 T2735 punct ",",8
R513 T2738 T2735 punct ],8
R514 T2739 T2712 punct .,translocates
R515 T2741 T2742 mark For,occur
R516 T2742 T2746 advcl occur,binds
R517 T2743 T2744 det this,process
R518 T2744 T2742 nsubj process,occur
R519 T2745 T2742 aux to,occur
R520 T2747 T2746 punct ", ",binds
R521 T2748 T2746 nsubj AVP,binds
R522 T2749 T2750 poss its,receptor
R523 T2750 T2746 dobj receptor,binds
R524 T2751 T2750 punct ", ",receptor
R525 T2752 T2750 appos AVPR2,receptor
R526 T2753 T2746 punct ", ",binds
R527 T2754 T2746 prep on,binds
R528 T2755 T2756 det the,face
R529 T2756 T2754 pobj face,on
R530 T2757 T2756 amod basolateral,face
R531 T2758 T2756 prep of,face
R532 T2759 T2760 det the,cells
R533 T2760 T2758 pobj cells,of
R534 T2761 T2762 amod collecting,duct
R535 T2762 T2760 compound duct,cells
R536 T2763 T2746 punct ", ",binds
R537 T2764 T2746 advcl leading,binds
R538 T2765 T2764 prep to,leading
R539 T2766 T2767 det a,rise
R540 T2767 T2765 pobj rise,to
R541 T2768 T2767 prep in,rise
R542 T2769 T2770 amod intracellular,cAMP
R543 T2770 T2768 pobj cAMP,in
R544 T2771 T2764 punct ", ",leading
R545 T2772 T2773 advmod ultimately,resulting
R546 T2773 T2764 advcl resulting,leading
R547 T2774 T2773 prep in,resulting
R548 T2775 T2774 pobj phosphorylation,in
R549 T2776 T2775 prep of,phosphorylation
R550 T2777 T2776 pobj AQP2,of
R551 T2778 T2775 prep at,phosphorylation
R552 T2779 T2778 pobj serine,at
R553 T2780 T2779 nummod 256,serine
R554 T2781 T2775 prep by,phosphorylation
R555 T2782 T2783 npadvmod cAMP,dependent
R556 T2783 T2785 amod dependent,kinase
R557 T2784 T2783 punct -,dependent
R558 T2785 T2781 pobj kinase,by
R559 T2786 T2785 compound protein,kinase
R560 T2787 T2788 punct [,9
R561 T2788 T2775 parataxis 9,phosphorylation
R562 T2789 T2788 punct ],9
R563 T2790 T2775 cc and,phosphorylation
R564 T2791 T2792 poss its,redistribution
R565 T2792 T2775 conj redistribution,phosphorylation
R566 T2793 T2792 prep to,redistribution
R567 T2794 T2795 det the,membrane
R568 T2795 T2793 pobj membrane,to
R569 T2796 T2795 compound plasma,membrane
R570 T2797 T2746 punct .,binds
R571 T2799 T2800 det The,importance
R572 T2800 T2801 nsubjpass importance,highlighted
R573 T2802 T2800 prep of,importance
R574 T2803 T2804 compound AQP2,redistribution
R575 T2804 T2802 pobj redistribution,of
R576 T2805 T2801 aux has,highlighted
R577 T2806 T2801 auxpass been,highlighted
R578 T2807 T2801 agent by,highlighted
R579 T2808 T2809 amod functional,characterization
R580 T2809 T2807 pobj characterization,by
R581 T2810 T2809 prep of,characterization
R582 T2811 T2812 compound Aqp2,mutations
R583 T2812 T2810 pobj mutations,of
R584 T2813 T2801 advcl resulting,highlighted
R585 T2814 T2813 prep in,resulting
R586 T2815 T2816 amod severe,NDI
R587 T2816 T2814 pobj NDI,in
R588 T2817 T2813 prep in,resulting
R589 T2818 T2817 pobj humans,in
R590 T2819 T2820 punct [,10
R591 T2820 T2801 parataxis 10,highlighted
R592 T2821 T2820 nummod 3,10
R593 T2822 T2820 punct ",",10
R594 T2823 T2820 punct ],10
R595 T2824 T2801 punct .,highlighted
R596 T2826 T2827 amod Recessive,mutations
R597 T2827 T2829 nsubjpass mutations,thought
R598 T2828 T2827 compound Aqp2,mutations
R599 T2830 T2829 auxpass are,thought
R600 T2831 T2829 advmod generally,thought
R601 T2832 T2833 aux to,produce
R602 T2833 T2829 xcomp produce,thought
R603 T2834 T2835 det an,pore
R604 T2835 T2833 dobj pore,produce
R605 T2836 T2837 advmod abnormally,localized
R606 T2837 T2835 amod localized,pore
R607 T2838 T2837 cc and,localized
R608 T2839 T2840 punct ", ",misfolded
R609 T2840 T2837 conj misfolded,localized
R610 T2841 T2840 prep in,misfolded
R611 T2842 T2843 amod most,instances
R612 T2843 T2841 pobj instances,in
R613 T2844 T2840 punct ", ",misfolded
R614 T2845 T2835 compound water,pore
R615 T2846 T2847 dep that,responds
R616 T2847 T2835 relcl responds,pore
R617 T2848 T2847 advmod abnormally,responds
R618 T2849 T2847 prep to,responds
R619 T2850 T2851 det an,increase
R620 T2851 T2849 pobj increase,to
R621 T2852 T2851 prep in,increase
R622 T2853 T2852 pobj cAMP,in
R623 T2854 T2855 punct [,11
R624 T2855 T2829 parataxis 11,thought
R625 T2856 T2855 nummod 6,11
R626 T2857 T2855 punct ",",11
R627 T2858 T2855 punct ],11
R628 T2859 T2829 punct .,thought
R629 T2861 T2862 advmod Furthermore,described
R630 T2863 T2862 punct ", ",described
R631 T2864 T2865 amod dominant,mutations
R632 T2865 T2862 nsubjpass mutations,described
R633 T2866 T2862 aux have,described
R634 T2867 T2862 auxpass been,described
R635 T2868 T2862 cc and,described
R636 T2869 T2862 conj found,described
R637 T2870 T2871 aux to,misroute
R638 T2871 T2869 xcomp misroute,found
R639 T2872 T2873 preconj both,mutant
R640 T2873 T2871 dobj mutant,misroute
R641 T2874 T2873 det the,mutant
R642 T2875 T2873 cc and,mutant
R643 T2876 T2877 det the,type
R644 T2877 T2880 compound type,protein
R645 T2878 T2877 amod wild,type
R646 T2879 T2877 punct -,type
R647 T2880 T2873 conj protein,mutant
R648 T2881 T2871 prep to,misroute
R649 T2882 T2883 det the,membrane
R650 T2883 T2881 pobj membrane,to
R651 T2884 T2883 amod basolateral,membrane
R652 T2885 T2886 punct [,12
R653 T2886 T2869 parataxis 12,found
R654 T2887 T2886 nummod 6,12
R655 T2888 T2886 punct ",",12
R656 T2889 T2886 punct ],12
R657 T2890 T2862 punct .,described
R658 T2892 T2893 amod Several,models
R659 T2893 T2895 nsubjpass models,generated
R660 T2894 T2893 compound mouse,models
R661 T2896 T2893 prep of,models
R662 T2897 T2898 compound diabetes,insipidus
R663 T2898 T2896 pobj insipidus,of
R664 T2899 T2895 aux have,generated
R665 T2900 T2895 auxpass been,generated
R666 T2901 T2902 punct [,13
R667 T2902 T2895 parataxis 13,generated
R668 T2903 T2904 punct –,17
R669 T2904 T2902 prep 17,13
R670 T2905 T2902 punct ],13
R671 T2906 T2895 punct .,generated
R672 T2908 T2909 prep In,generated
R673 T2910 T2911 det an,attempt
R674 T2911 T2908 pobj attempt,In
R675 T2912 T2913 aux to,recapitulate
R676 T2913 T2911 acl recapitulate,attempt
R677 T2914 T2915 amod human,NDI
R678 T2915 T2913 dobj NDI,recapitulate
R679 T2916 T2909 punct ", ",generated
R680 T2917 T2909 nsubjpass mice,generated
R681 T2918 T2909 aux have,generated
R682 T2919 T2909 auxpass been,generated
R683 T2920 T2909 prep with,generated
R684 T2921 T2920 pobj mutations,with
R685 T2922 T2921 prep in,mutations
R686 T2923 T2922 pobj Aqp2,in
R687 T2924 T2923 cc and,Aqp2
R688 T2925 T2923 conj Avpr2,Aqp2
R689 T2926 T2927 punct [,18
R690 T2927 T2909 parataxis 18,generated
R691 T2928 T2927 nummod 15,18
R692 T2929 T2927 punct ",",18
R693 T2930 T2927 punct ],18
R694 T2931 T2909 punct .,generated
R695 T2933 T2934 nsubj Yang,created
R696 T2935 T2933 cc and,Yang
R697 T2936 T2933 conj colleagues,Yang
R698 T2937 T2938 det a,mouse
R699 T2938 T2934 dobj mouse,created
R700 T2939 T2938 prep with,mouse
R701 T2940 T2941 det a,mutation
R702 T2941 T2939 pobj mutation,with
R703 T2942 T2941 nmod T126M,mutation
R704 T2943 T2941 amod knock,mutation
R705 T2944 T2943 punct -,knock
R706 T2945 T2943 prt in,knock
R707 T2946 T2941 prep in,mutation
R708 T2947 T2948 det the,gene
R709 T2948 T2946 pobj gene,in
R710 T2949 T2948 compound Aqp2,gene
R711 T2950 T2934 punct .,created
R712 T2952 T2953 advmod Unexpectedly,died
R713 T2954 T2953 punct ", ",died
R714 T2955 T2956 amod homozygous,mice
R715 T2956 T2953 nsubj mice,died
R716 T2957 T2956 compound mutant,mice
R717 T2958 T2953 prep within,died
R718 T2959 T2960 nummod 6,d
R719 T2960 T2958 pobj d,within
R720 T2961 T2960 prep after,d
R721 T2962 T2961 pobj birth,after
R722 T2963 T2953 punct .,died
R723 T2965 T2966 advmod Interestingly,die
R724 T2967 T2966 punct ", ",die
R725 T2968 T2969 npadvmod AVPR2,deficient
R726 T2969 T2971 amod deficient,pups
R727 T2970 T2969 punct -,deficient
R728 T2971 T2966 nsubj pups,die
R729 T2972 T2971 amod male,pups
R730 T2973 T2966 advmod also,die
R731 T2974 T2966 prep within,die
R732 T2975 T2976 det the,week
R733 T2976 T2974 pobj week,within
R734 T2977 T2976 amod first,week
R735 T2978 T2976 prep after,week
R736 T2979 T2978 pobj birth,after
R737 T2980 T2966 punct .,die
R738 T2982 T2983 advmod Together,suggest
R739 T2984 T2985 det these,models
R740 T2985 T2983 nsubj models,suggest
R741 T2986 T2987 mark that,be
R742 T2987 T2983 ccomp be,suggest
R743 T2988 T2989 det the,mouse
R744 T2989 T2987 nsubj mouse,be
R745 T3096 T3095 punct -,duct
R746 T2990 T2987 aux may,be
R747 T3097 T3084 punct ", ",adopts
R748 T2991 T2992 det a,organism
R749 T2992 T2987 attr organism,be
R750 T3098 T3084 cc and,adopts
R751 T2993 T2994 advmod highly,sensitive
R752 T2994 T2992 amod sensitive,organism
R753 T3099 T3084 conj was,adopts
R754 T2995 T2987 prep with,be
R755 T2996 T2995 pobj regard,with
R756 T3100 T3099 acomp resistant,was
R757 T2997 T2996 prep to,regard
R758 T2998 T2999 compound water,homeostasis
R759 T2999 T2997 pobj homeostasis,to
R760 T3000 T2987 punct ", ",be
R761 T3001 T2987 cc and,be
R762 T3101 T3100 prep to,resistant
R763 T3002 T2987 conj is,be
R764 T3003 T3002 acomp unable,is
R765 T3004 T3005 aux to,survive
R766 T3102 T3101 pobj translocation,to
R767 T3005 T3003 xcomp survive,unable
R768 T3006 T3005 prep with,survive
R769 T3007 T3006 pobj polyuria,with
R770 T3103 T3102 acl induced,translocation
R771 T3008 T2983 punct .,suggest
R772 T3010 T3011 prep In,identified
R773 T3104 T3103 agent by,induced
R774 T3012 T3013 det a,screen
R775 T3105 T3104 pobj desmopressin,by
R776 T3013 T3010 pobj screen,In
R777 T3014 T3015 advmod forward,genetic
R778 T3015 T3013 amod genetic,screen
R779 T3016 T3011 punct ", ",identified
R780 T3106 T3105 punct ", ",desmopressin
R781 T3017 T3018 det a,mouse
R782 T3018 T3011 nsubjpass mouse,identified
R783 T3107 T3108 det an,agonist
R784 T3019 T3018 prep with,mouse
R785 T3020 T3021 det an,mutation
R786 T3021 T3019 pobj mutation,with
R787 T3108 T3105 appos agonist,desmopressin
R788 T3109 T3108 prep of,agonist
R789 T3022 T3021 compound Aqp2,mutation
R790 T3023 T3011 auxpass was,identified
R791 T3024 T3011 punct .,identified
R792 T3026 T3027 det The,purpose
R793 T3110 T3109 pobj AVP,of
R794 T3027 T3028 nsubj purpose,was
R795 T3029 T3027 prep of,purpose
R796 T3030 T3031 det this,study
R797 T3111 T3082 punct .,concluded
R798 T3031 T3029 pobj study,of
R799 T3032 T3033 aux to,characterize
R800 T3033 T3028 xcomp characterize,was
R801 T3113 T3114 advmod In,vitro
R802 T3034 T3035 det this,model
R803 T3035 T3033 dobj model,characterize
R804 T3036 T3035 amod murine,model
R805 T3037 T3035 prep of,model
R806 T3114 T3115 amod vitro,studies
R807 T3038 T3039 amod recessive,DI
R808 T3039 T3037 pobj DI,of
R809 T3115 T3116 nsubj studies,demonstrated
R810 T3040 T3039 amod nephrogenic,DI
R811 T3041 T3028 punct .,was
R812 T3043 T3044 nsubj We,report
R813 T3045 T3044 advmod now,report
R814 T3117 T3115 acl using,studies
R815 T3046 T3047 det a,mutation
R816 T3047 T3044 dobj mutation,report
R817 T3048 T3047 amod novel,mutation
R818 T3049 T3047 compound F204V,mutation
R819 T3118 T3119 det the,line
R820 T3050 T3047 prep in,mutation
R821 T3051 T3052 det the,gene
R822 T3052 T3050 pobj gene,in
R823 T3119 T3117 dobj line,using
R824 T3053 T3052 compound Aqp2,gene
R825 T3054 T3044 punct .,report
R826 T3120 T3121 nmod Madin,Darby
R827 T3056 T3057 det This,allele
R828 T3057 T3058 nsubjpass allele,found
R829 T3121 T3123 nmod Darby,kidney
R830 T3059 T3057 prep of,allele
R831 T3060 T3059 pobj Aqp2,of
R832 T3122 T3121 punct -,Darby
R833 T3061 T3058 auxpass was,found
R834 T3062 T3063 aux to,cause
R835 T3063 T3058 xcomp cause,found
R836 T3123 T3119 nmod kidney,line
R837 T3064 T3065 det the,model
R838 T3065 T3068 nsubj model,survive
R839 T3124 T3123 amod canine,kidney
R840 T3066 T3065 amod first,model
R841 T3067 T3065 compound mouse,model
R842 T3125 T3123 punct (,kidney
R843 T3068 T3063 ccomp survive,cause
R844 T3069 T3065 prep of,model
R845 T3070 T3069 pobj NDI,of
R846 T3126 T3123 appos MDCK,kidney
R847 T3071 T3068 aux to,survive
R848 T3072 T3068 prep past,survive
R849 T3073 T3074 det the,week
R850 T3127 T3119 punct ),line
R851 T3074 T3072 pobj week,past
R852 T3075 T3074 amod first,week
R853 T3076 T3074 prep of,week
R854 T3128 T3119 compound cell,line
R855 T3129 T3130 det an,pattern
R856 T3130 T3116 dobj pattern,demonstrated
R857 T3077 T3076 pobj life,of
R858 T3078 T3058 punct .,found
R859 T3131 T3130 amod endoplasmic,pattern
R860 T3080 T3081 amod Molecular,analyses
R861 T3132 T3130 compound reticulum,pattern
R862 T3081 T3082 nsubj analyses,concluded
R863 T3083 T3084 mark that,adopts
R864 T3084 T3082 ccomp adopts,concluded
R865 T3085 T3086 compound mutant,AQP2
R866 T3086 T3084 nsubj AQP2,adopts
R867 T3133 T3130 prep for,pattern
R868 T3087 T3088 det a,localization
R869 T3088 T3084 dobj localization,adopts
R870 T3089 T3088 amod different,localization
R871 T3134 T3135 det the,protein
R872 T3090 T3088 amod subcellular,localization
R873 T3091 T3084 prep in,adopts
R874 T3092 T3093 amod renal,cells
R875 T3135 T3133 pobj protein,for
R876 T3093 T3091 pobj cells,in
R877 T3094 T3095 amod collecting,duct
R878 T3136 T3135 compound mutant,protein
R879 T3095 T3093 compound duct,cells
R880 T3137 T3130 punct ", ",pattern
R881 T3138 T3130 cc and,pattern
R882 T3139 T3140 amod apparent,resistance
R883 T3140 T3130 conj resistance,pattern
R884 T3141 T3140 prep to,resistance
R885 T3142 T3141 pobj translocation,to
R886 T3143 T3116 punct .,demonstrated
R887 T3145 T3146 det These,data
R888 T3146 T3147 nsubj data,prove
R889 T3148 T3147 advmod conclusively,prove
R890 T3149 T3150 mark that,is
R891 T3150 T3147 ccomp is,prove
R892 T3151 T3152 amod autosomal,NDI
R893 T3152 T3150 nsubj NDI,is
R894 T3153 T3152 amod recessive,NDI
R895 T3154 T3155 det a,consequence
R896 T3155 T3150 attr consequence,is
R897 T3156 T3155 prep of,consequence
R898 T3157 T3158 amod improper,routing
R899 T3158 T3156 pobj routing,of
R900 T3159 T3158 compound AQP2,routing
R901 T3160 T3158 prep in,routing
R902 T3161 T3162 det the,mammal
R903 T3162 T3160 pobj mammal,in
R904 T3163 T3162 amod intact,mammal
R905 T3164 T3147 punct .,prove
R916 T8980 T8981 punct [,20
R917 T8951 T8952 prep In,found
R918 T8953 T8954 det a,screen
R919 T8954 T8951 pobj screen,In
R920 T8955 T8956 amod forward,genetic
R921 T8956 T8954 amod genetic,screen
R922 T8957 T8958 dep that,used
R923 T8958 T8954 relcl used,screen
R924 T8959 T8958 dobj ethylnitrosourea,used
R925 T8960 T8959 punct (,ethylnitrosourea
R926 T8961 T8959 appos ENU,ethylnitrosourea
R927 T8981 T8958 parataxis 20,used
R928 T8962 T8958 punct ),used
R929 T8963 T8964 aux to,induce
R930 T8964 T8958 advcl induce,used
R931 T8982 T8981 nummod 19,20
R932 T8965 T8964 dobj mutations,induce
R933 T8966 T8964 prep in,induce
R934 T8967 T8968 det a,animal
R935 T8983 T8981 punct ",",20
R936 T8968 T8966 pobj animal,in
R937 T8969 T8968 compound founder,animal
R938 T8970 T8971 poss whose,offspring
R939 T8984 T8981 punct ],20
R940 T8971 T8972 dep offspring,screened
R941 T8972 T8968 relcl screened,animal
R942 T8973 T8972 auxpass were,screened
R943 T8985 T8952 punct ", ",found
R944 T8974 T8972 advmod then,screened
R945 T8986 T8952 nsubj we,found
R946 T8975 T8972 prep for,screened
R947 T8976 T8977 amod abnormal,metabolism
R948 T8987 T8988 det a,family
R949 T8977 T8975 pobj metabolism,for
R950 T8978 T8979 amod whole,body
R951 T8988 T8952 dobj family,found
R952 T8979 T8977 compound body,metabolism
R953 T8989 T8988 prep of,family
R954 T8990 T8989 pobj mice,of
R955 T8991 T8992 dep that,urinated
R956 T9086 T9084 pobj substitution,to
R957 T9087 T9086 nmod valine,substitution
R958 T9088 T9087 prep for,valine
R959 T9089 T9088 pobj phenylalanine,for
R960 T9090 T9086 prep at,substitution
R961 T8992 T8988 relcl urinated,family
R962 T9091 T9092 compound amino,acid
R963 T9092 T9090 pobj acid,at
R964 T9093 T9092 nummod 204,acid
R965 T9094 T9092 prep of,acid
R966 T8993 T8992 cc and,urinated
R967 T9095 T9096 det the,protein
R968 T9096 T9094 pobj protein,of
R969 T9097 T9092 punct (,acid
R970 T8994 T8992 conj drank,urinated
R971 T9098 T9092 appos F204V,acid
R972 T9099 T9092 punct ),acid
R973 T9100 T9056 punct .,identified
R974 T8995 T8994 advmod excessively,drank
R975 T8996 T8952 punct .,found
R976 T9102 T9103 nsubj AQP2,is
R977 T9104 T9105 det a,channel
R978 T8998 T8999 nmod Serum,analysis
R979 T9105 T9103 attr channel,is
R980 T9106 T9107 nummod six,transmembrane
R981 T9107 T9105 compound transmembrane,channel
R982 T9108 T9107 punct -,transmembrane
R983 T8999 T9002 nsubj analysis,showed
R984 T9109 T9105 compound water,channel
R985 T9110 T9103 punct ", ",is
R986 T9000 T8998 cc and,Serum
R987 T9111 T9103 cc and,is
R988 T9112 T9113 nsubj F204,lies
R989 T9113 T9103 conj lies,is
R990 T9001 T8998 conj urine,Serum
R991 T9114 T9113 prep near,lies
R992 T9115 T9116 det the,face
R993 T9003 T9004 mark that,were
R994 T9116 T9114 pobj face,near
R995 T9117 T9116 amod extracellular,face
R996 T9118 T9116 prep of,face
R997 T9119 T9120 det the,domain
R998 T9120 T9118 pobj domain,of
R999 T9004 T9002 ccomp were,showed
R1000 T9121 T9120 amod sixth,domain
R1001 T9005 T9006 compound plasma,levels
R1002 T9122 T9123 npadvmod membrane,spanning
R1003 T9123 T9120 amod spanning,domain
R1004 T9124 T9120 punct ", ",domain
R1005 T9125 T9126 det a,region
R1006 T9006 T9004 nsubj levels,were
R1007 T9126 T9120 appos region,domain
R1008 T9127 T9126 amod rich,region
R1009 T9128 T9127 prep in,rich
R1010 T9129 T9130 amod hydrophobic,acids
R1011 T9130 T9128 pobj acids,in
R1012 T9131 T9130 compound amino,acids
R1013 T9007 T9006 compound glucose,levels
R1014 T9132 T9113 punct .,lies
R1015 T9134 T9135 nsubjpass This,conserved
R1016 T9008 T9004 acomp normal,were
R1017 T9136 T9134 cc and,This
R1018 T9137 T9138 det the,other
R1019 T9009 T9004 cc and,were
R1020 T9138 T9139 nmod other,domains
R1021 T9139 T9134 conj domains,This
R1022 T9140 T9141 npadvmod membrane,spanning
R1023 T9010 T9011 expl there,was
R1024 T9141 T9139 amod spanning,domains
R1025 T9142 T9141 punct -,spanning
R1026 T9011 T9004 conj was,were
R1027 T9143 T9135 auxpass are,conserved
R1028 T9144 T9135 prep among,conserved
R1029 T9145 T9146 amod vertebrate,species
R1030 T9012 T9013 det no,glucose
R1031 T9146 T9144 pobj species,among
R1032 T9147 T9135 punct .,conserved
R1033 T9013 T9011 attr glucose,was
R1034 T9149 T9150 det The,phenylalanine
R1035 T9150 T9151 nsubj phenylalanine,is
R1036 T9014 T9011 prep in,was
R1037 T9152 T9150 prep at,phenylalanine
R1038 T9015 T9016 det the,urine
R1039 T9016 T9014 pobj urine,in
R1040 T9153 T9152 pobj position,at
R1041 T9154 T9153 nummod 204,position
R1042 T9017 T9018 punct (,data
R1043 T9155 T9156 advmod particularly,conserved
R1044 T9156 T9151 acomp conserved,is
R1045 T9018 T9002 meta data,showed
R1046 T9157 T9156 advmod well,conserved
R1047 T9158 T9159 punct (,Figure
R1048 T9159 T9156 parataxis Figure,conserved
R1049 T9019 T9018 amod unpublished,data
R1050 T9160 T9159 nummod 1B,Figure
R1051 T9161 T9159 punct ),Figure
R1052 T9020 T9018 punct ),data
R1053 T9162 T9151 punct ", ",is
R1054 T9163 T9164 preconj not,among
R1055 T9164 T9151 prep among,is
R1056 T9021 T9002 punct .,showed
R1057 T9165 T9163 advmod only,not
R1058 T9166 T9167 amod vertebrate,proteins
R1059 T9023 T9024 advmod Hence,was
R1060 T9167 T9164 pobj proteins,among
R1061 T9168 T9167 compound AQP2,proteins
R1062 T9169 T9164 punct ", ",among
R1063 T9170 T9164 cc but,among
R1064 T9171 T9170 advmod also,but
R1065 T9172 T9164 conj among,among
R1066 T9173 T9174 compound others,members
R1067 T9025 T9024 punct ", ",was
R1068 T9174 T9172 pobj members,among
R1069 T9175 T9174 prep of,members
R1070 T9176 T9177 det this,family
R1071 T9177 T9175 pobj family,of
R1072 T9178 T9151 punct .,is
R1073 T9026 T9024 nsubj this,was
R1074 T9180 T9181 compound Aqp2F204V,F204V
R1075 T9181 T9183 compound F204V,mice
R1076 T9027 T9028 det an,example
R1077 T9182 T9181 punct /,F204V
R1078 T9183 T9184 nsubj mice,have
R1079 T9028 T9024 attr example,was
R1080 T9185 T9186 advmod dramatically,increased
R1081 T9186 T9187 amod increased,production
R1082 T9029 T9028 prep of,example
R1083 T9187 T9184 dobj production,have
R1084 T9188 T9187 compound urine,production
R1085 T9189 T9184 punct ", ",have
R1086 T9030 T9031 compound diabetes,insipidus
R1087 T9190 T9191 prep in,producing
R1088 T9191 T9184 advcl producing,have
R1089 T9031 T9029 pobj insipidus,of
R1090 T9032 T9024 punct .,was
R1091 T9034 T9035 det The,disorder
R1092 T9192 T9193 det some,cases
R1093 T9193 T9190 pobj cases,in
R1094 T9194 T9195 det an,amount
R1095 T9035 T9036 nsubj disorder,segregated
R1096 T9195 T9191 dobj amount,producing
R1097 T9196 T9195 prep of,amount
R1098 T9197 T9196 pobj urine,of
R1099 T9037 T9035 prep in,disorder
R1100 T9198 T9191 prep in,producing
R1101 T9199 T9200 nummod 24,h
R1102 T9200 T9198 pobj h,in
R1103 T9201 T9202 dep that,exceeds
R1104 T9038 T9039 det these,mice
R1105 T9202 T9191 ccomp exceeds,producing
R1106 T9203 T9204 poss their,weight
R1107 T9204 T9202 dobj weight,exceeds
R1108 T9039 T9037 pobj mice,in
R1109 T9205 T9204 compound body,weight
R1110 T9206 T9191 punct ", ",producing
R1111 T9040 T9036 prep in,segregated
R1112 T9207 T9191 prep compared,producing
R1113 T9208 T9207 prep to,compared
R1114 T9209 T9210 poss their,littermates
R1115 T9210 T9208 pobj littermates,to
R1116 T9211 T9210 amod heterozygous,littermates
R1117 T9212 T9211 cc or,heterozygous
R1118 T9041 T9042 det a,manner
R1119 T9213 T9214 amod wild,type
R1120 T9214 T9211 conj type,heterozygous
R1121 T9215 T9214 punct -,type
R1122 T9042 T9040 pobj manner,in
R1123 T9216 T9184 punct .,have
R1124 T9043 T9042 amod monogenic,manner
R1125 T9218 T9219 amod Such,loss
R1126 T9219 T9220 nsubj loss,lead
R1127 T9044 T9042 punct ", ",manner
R1128 T9221 T9219 prep of,loss
R1129 T9222 T9221 pobj water,of
R1130 T9223 T9220 aux would,lead
R1131 T9224 T9220 advmod rapidly,lead
R1132 T9045 T9046 amod autosomal,recessive
R1133 T9225 T9220 prep to,lead
R1134 T9226 T9225 pobj dehydration,to
R1135 T9227 T9228 auxpass were,compensated
R1136 T9046 T9042 amod recessive,manner
R1137 T9228 T9220 advcl compensated,lead
R1138 T9229 T9228 nsubjpass it,compensated
R1139 T9047 T9036 punct ", ",segregated
R1140 T9230 T9228 neg not,compensated
R1141 T9231 T9228 agent by,compensated
R1142 T9232 T9233 amod increased,intake
R1143 T9048 T9036 advcl making,segregated
R1144 T9233 T9231 pobj intake,by
R1145 T9234 T9233 compound water,intake
R1146 T9235 T9220 punct .,lead
R1147 T9049 T9050 nsubj Aqp2,gene
R1148 T9237 T9238 advmod Indeed,increase
R1149 T9050 T9048 ccomp gene,making
R1150 T9239 T9238 punct ", ",increase
R1151 T9051 T9050 det a,gene
R1152 T9240 T9241 compound mutant,mice
R1153 T9052 T9050 compound candidate,gene
R1154 T9241 T9238 nsubj mice,increase
R1155 T9242 T9238 advmod also,increase
R1156 T9053 T9036 punct .,segregated
R1157 T9243 T9238 advmod dramatically,increase
R1158 T9244 T9245 poss their,intake
R1159 T9245 T9238 dobj intake,increase
R1160 T9055 T9056 nsubj Sequencing,identified
R1161 T9246 T9245 compound water,intake
R1162 T9247 T9248 punct (,Figure
R1163 T9248 T9238 parataxis Figure,increase
R1164 T9249 T9248 nummod 1C,Figure
R1165 T9057 T9055 prep of,Sequencing
R1166 T9250 T9248 punct ),Figure
R1167 T9251 T9238 prep compared,increase
R1168 T9252 T9251 prep to,compared
R1169 T9253 T9254 poss their,littermates
R1170 T9254 T9252 pobj littermates,to
R1171 T9058 T9059 compound Aqp2,region
R1172 T9255 T9254 amod heterozygous,littermates
R1173 T9256 T9255 cc or,heterozygous
R1174 T9257 T9258 amod wild,type
R1175 T9059 T9057 pobj region,of
R1176 T9258 T9255 conj type,heterozygous
R1177 T9259 T9258 punct -,type
R1178 T9260 T9238 punct .,increase
R1179 T9060 T9059 compound coding,region
R1180 T9262 T9263 det This,phenotype
R1181 T9061 T9059 prep of,region
R1182 T9263 T9264 nsubj phenotype,showed
R1183 T9265 T9263 punct —,phenotype
R1184 T9062 T9063 amod affected,mice
R1185 T9266 T9267 amod increased,output
R1186 T9267 T9263 appos output,phenotype
R1187 T9268 T9267 amod urinary,output
R1188 T9063 T9061 pobj mice,of
R1189 T9269 T9267 cc and,output
R1190 T9270 T9271 compound water,intake
R1191 T9271 T9267 conj intake,output
R1192 T9064 T9065 det a,transversion
R1193 T9272 T9264 punct —,showed
R1194 T9273 T9274 amod complete,concordance
R1195 T9274 T9264 dobj concordance,showed
R1196 T9065 T9056 dobj transversion,identified
R1197 T9275 T9274 prep with,concordance
R1198 T9276 T9275 pobj homozygosity,with
R1199 T9277 T9276 prep of,homozygosity
R1200 T9066 T9065 nmod thymine,transversion
R1201 T9278 T9279 det the,mutation
R1202 T9279 T9277 pobj mutation,of
R1203 T9280 T9279 compound F204V,mutation
R1204 T9067 T9066 prep to,thymine
R1205 T9281 T9264 prep in,showed
R1206 T9282 T9283 det the,animals
R1207 T9283 T9281 pobj animals,in
R1208 T9068 T9067 pobj guanine,to
R1209 T9284 T9283 nummod 58,animals
R1210 T9285 T9283 acl tested,animals
R1211 T9286 T9264 punct .,showed
R1212 T9069 T9066 punct (,thymine
R1213 T9288 T9289 compound Diabetes,insipidus
R1214 T9289 T9290 nsubjpass insipidus,defined
R1215 T9070 T9066 appos T,thymine
R1216 T9291 T9290 aux can,defined
R1217 T9292 T9290 auxpass be,defined
R1218 T9293 T9290 prep as,defined
R1219 T9294 T9295 det an,inability
R1220 T9295 T9293 pobj inability,as
R1221 T9071 T9070 prep to,T
R1222 T9296 T9297 aux to,concentrate
R1223 T9297 T9295 acl concentrate,inability
R1224 T9072 T9071 pobj G,to
R1225 T9073 T9065 punct ),transversion
R1226 T9074 T9075 punct (,Figure
R1227 T9298 T9297 dobj urine,concentrate
R1228 T9299 T9300 advmod where,appropriate
R1229 T9075 T9065 parataxis Figure,transversion
R1230 T9300 T9297 advcl appropriate,concentrate
R1231 T9301 T9290 punct .,defined
R1232 T9076 T9075 nummod 1A,Figure
R1233 T9303 T9304 prep Compared,produce
R1234 T9305 T9303 prep to,Compared
R1235 T9077 T9075 punct ),Figure
R1236 T9306 T9307 amod wild,type
R1237 T9307 T9309 nmod type,littermates
R1238 T9308 T9307 punct -,type
R1239 T9078 T9065 punct ", ",transversion
R1240 T9309 T9305 pobj littermates,to
R1241 T9310 T9307 cc or,type
R1242 T9311 T9307 conj heterozygous,type
R1243 T9079 T9080 dep which,predicted
R1244 T9312 T9304 punct ", ",produce
R1245 T9313 T9314 compound Aqp2F204V,F204V
R1246 T9314 T9316 compound F204V,mice
R1247 T9080 T9065 relcl predicted,transversion
R1248 T9315 T9314 punct /,F204V
R1249 T9316 T9304 nsubj mice,produce
R1250 T9317 T9318 advmod very,dilute
R1251 T9318 T9319 amod dilute,urine
R1252 T9081 T9080 auxpass is,predicted
R1253 T9319 T9304 dobj urine,produce
R1254 T9320 T9321 punct (,Figure
R1255 T9321 T9304 parataxis Figure,produce
R1256 T9082 T9083 aux to,lead
R1257 T9322 T9321 nummod 1D,Figure
R1258 T9323 T9321 punct ),Figure
R1259 T9083 T9080 xcomp lead,predicted
R1260 T9324 T9304 punct .,produce
R1261 T9326 T9327 amod Basal,concentration
R1262 T9084 T9083 prep to,lead
R1263 T9085 T9086 det a,substitution
R1264 T9327 T9329 nsubj concentration,is
R1265 T9403 T9402 oprd desmopressin,called
R1266 T9328 T9327 compound urine,concentration
R1267 T9330 T9327 prep in,concentration
R1268 T9331 T9332 compound mutant,mice
R1269 T9332 T9330 pobj mice,in
R1270 T9333 T9334 advmod about,161
R1271 T9404 T9383 punct ),analog
R1272 T9334 T9335 nummod 161,mOsm
R1273 T9335 T9329 attr mOsm,is
R1274 T9405 T9386 punct ", ",is
R1275 T9336 T9335 punct ", ",mOsm
R1276 T9337 T9335 prep compared,mOsm
R1277 T9338 T9337 prep to,compared
R1278 T9406 T9407 det a,agonist
R1279 T9339 T9340 quantmod about,"1,293"
R1280 T9340 T9341 nummod "1,293",mOsm
R1281 T9341 T9338 pobj mOsm,to
R1282 T9407 T9386 attr agonist,is
R1283 T9342 T9341 prep in,mOsm
R1284 T9343 T9344 amod wild,type
R1285 T9344 T9346 compound type,mice
R1286 T9408 T9407 amod potent,agonist
R1287 T9345 T9344 punct -,type
R1288 T9346 T9342 pobj mice,in
R1289 T9409 T9407 prep of,agonist
R1290 T9347 T9348 punct (,0.001
R1291 T9348 T9329 parataxis 0.001,is
R1292 T9349 T9348 nsubj p,0.001
R1293 T9410 T9409 pobj AVPR2,of
R1294 T9350 T9348 punct <,0.001
R1295 T9351 T9348 punct ),0.001
R1296 T9352 T9329 punct .,is
R1297 T9411 T9386 punct .,is
R1298 T9354 T9355 advmod Normally,is
R1299 T9413 T9414 advmod When,administered
R1300 T9356 T9355 punct ", ",is
R1301 T9357 T9358 compound urine,concentration
R1302 T9358 T9355 nsubj concentration,is
R1303 T9359 T9355 prep under,is
R1304 T9360 T9361 det the,control
R1305 T9361 T9359 pobj control,under
R1306 T9414 T9415 advcl administered,leads
R1307 T9362 T9361 prep of,control
R1308 T9363 T9364 det the,hypothalamus
R1309 T9364 T9362 pobj hypothalamus,of
R1310 T9365 T9364 punct ", ",hypothalamus
R1311 T9416 T9414 prep to,administered
R1312 T9366 T9367 dep which,secretes
R1313 T9367 T9364 relcl secretes,hypothalamus
R1314 T9368 T9367 punct ", ",secretes
R1315 T9369 T9367 prep in,secretes
R1316 T9417 T9418 amod wild,type
R1317 T9370 T9369 pobj response,in
R1318 T9371 T9370 prep to,response
R1319 T9372 T9371 pobj hypovolemia,to
R1320 T9373 T9372 cc or,hypovolemia
R1321 T9374 T9372 conj hypernatremia,hypovolemia
R1322 T9375 T9376 punct [,21
R1323 T9418 T9420 compound type,mice
R1324 T9376 T9370 parataxis 21,response
R1325 T9377 T9376 punct ],21
R1326 T9378 T9367 punct ", ",secretes
R1327 T9379 T9367 dobj AVP,secretes
R1328 T9419 T9418 punct -,type
R1329 T9380 T9355 punct .,is
R1330 T9382 T9383 det The,analog
R1331 T9420 T9416 pobj mice,to
R1332 T9383 T9386 nsubj analog,is
R1333 T9384 T9383 amod synthetic,analog
R1334 T9421 T9415 punct ", ",leads
R1335 T9385 T9383 compound AVP,analog
R1336 T9387 T9383 punct ", ",analog
R1337 T9422 T9415 nsubj dDAVP,leads
R1338 T9388 T9389 nummod 1,deamino
R1339 T9389 T9391 nmod deamino,arginine
R1340 T9390 T9389 punct -,deamino
R1341 T9423 T9415 prep to,leads
R1342 T9424 T9425 det a,increase
R1343 T9391 T9397 compound arginine,vasopressin
R1344 T9425 T9423 pobj increase,to
R1345 T9392 T9391 punct -,arginine
R1346 T9393 T9391 nummod 8,arginine
R1347 T9426 T9425 amod dramatic,increase
R1348 T9394 T9391 punct -,arginine
R1349 T9395 T9391 compound D,arginine
R1350 T9396 T9391 punct -,arginine
R1351 T9427 T9425 prep in,increase
R1352 T9397 T9383 appos vasopressin,analog
R1353 T9398 T9397 punct (,vasopressin
R1354 T9399 T9397 appos dDAVP,vasopressin
R1355 T9428 T9429 compound urine,concentration
R1356 T9400 T9397 punct ;,vasopressin
R1357 T9401 T9402 advmod also,called
R1358 T9402 T9397 acl called,vasopressin
R1359 T9429 T9427 pobj concentration,in
R1360 T9430 T9425 punct ", ",increase
R1361 T9431 T9425 prep from,increase
R1362 T9432 T9431 pobj "1,293",from
R1363 T9509 T9510 dep which,be
R1364 T9510 T9505 relcl be,channel
R1365 T9433 T9431 prep to,from
R1366 T9511 T9510 aux must,be
R1367 T9512 T9510 acomp sufficient,be
R1368 T9513 T9514 aux to,allow
R1369 T9434 T9433 pobj "5,885",to
R1370 T9514 T9512 xcomp allow,sufficient
R1371 T9515 T9514 dobj survival,allow
R1372 T9516 T9515 prep of,survival
R1373 T9517 T9518 det the,individual
R1374 T9518 T9516 pobj individual,of
R1375 T9519 T9497 punct ", ",indicates
R1376 T9435 T9431 pobj mOsm,from
R1377 T9520 T9497 prep in,indicates
R1378 T9521 T9520 pobj contrast,in
R1379 T9522 T9521 prep to,contrast
R1380 T9436 T9437 punct (,Figure
R1381 T9523 T9524 det the,mouse
R1382 T9524 T9522 pobj mouse,to
R1383 T9437 T9415 parataxis Figure,leads
R1384 T9525 T9524 nmod T126M,mouse
R1385 T9526 T9524 amod knock,mouse
R1386 T9527 T9526 punct -,knock
R1387 T9528 T9526 prt in,knock
R1388 T9438 T9437 advmod 4.6-fold,Figure
R1389 T9529 T9530 punct [,18
R1390 T9530 T9497 parataxis 18,indicates
R1391 T9531 T9530 punct ],18
R1392 T9439 T9437 punct ;,Figure
R1393 T9532 T9497 punct .,indicates
R1394 T9534 T9535 amod Multiple,matings
R1395 T9440 T9437 nummod 1D,Figure
R1396 T9535 T9537 nsubj matings,yielded
R1397 T9536 T9535 amod heterozygous,matings
R1398 T9441 T9437 punct ),Figure
R1399 T9538 T9539 nummod 101,animals
R1400 T9539 T9537 dobj animals,yielded
R1401 T9442 T9415 punct .,leads
R1402 T9540 T9539 punct ", ",animals
R1403 T9541 T9542 dep which,appeared
R1404 T9542 T9539 relcl appeared,animals
R1405 T9543 T9542 prep at,appeared
R1406 T9444 T9445 prep With,concentrate
R1407 T9544 T9545 det a,ratio
R1408 T9545 T9543 pobj ratio,at
R1409 T9546 T9545 prep of,ratio
R1410 T9547 T9546 pobj 26,of
R1411 T9446 T9447 amod similar,treatment
R1412 T9548 T9549 punct :,49
R1413 T9549 T9547 prep 49,26
R1414 T9550 T9551 punct :,26
R1415 T9447 T9444 pobj treatment,With
R1416 T9551 T9547 prep 26,26
R1417 T9552 T9542 punct ", ",appeared
R1418 T9553 T9542 prep near,appeared
R1419 T9448 T9445 punct ", ",concentrate
R1420 T9554 T9555 det the,frequencies
R1421 T9555 T9553 pobj frequencies,near
R1422 T9556 T9555 amod expected,frequencies
R1423 T9557 T9558 amod Mendelian,type
R1424 T9558 T9555 nmod type,frequencies
R1425 T9559 T9558 amod wild,type
R1426 T9449 T9450 compound mutant,mice
R1427 T9560 T9558 punct ", ",type
R1428 T9561 T9558 conj heterozygote,type
R1429 T9562 T9561 punct ", ",heterozygote
R1430 T9450 T9445 nsubj mice,concentrate
R1431 T9563 T9561 cc and,heterozygote
R1432 T9564 T9561 conj mutant,heterozygote
R1433 T9565 T9542 punct ", ",appeared
R1434 T9451 T9452 poss their,urine
R1435 T9566 T9542 advmod respectively,appeared
R1436 T9567 T9537 punct ", ",yielded
R1437 T9452 T9445 dobj urine,concentrate
R1438 T9568 T9537 advcl indicating,yielded
R1439 T9569 T9570 mark that,is
R1440 T9570 T9568 ccomp is,indicating
R1441 T9571 T9570 expl there,is
R1442 T9453 T9445 prep to,concentrate
R1443 T9572 T9573 det no,viability
R1444 T9573 T9570 attr viability,is
R1445 T9454 T9455 det a,extent
R1446 T9574 T9573 amod reduced,viability
R1447 T9575 T9573 acl associated,viability
R1448 T9576 T9575 prep with,associated
R1449 T9455 T9453 pobj extent,to
R1450 T9577 T9578 det this,mutation
R1451 T9578 T9576 pobj mutation,with
R1452 T9579 T9537 punct .,yielded
R1453 T9456 T9455 amod lesser,extent
R1454 T9581 T9582 amod Other,was
R1455 T9457 T9456 cc but,lesser
R1456 T9583 T9581 prep than,Other
R1457 T9458 T9459 advmod still,significant
R1458 T9459 T9456 conj significant,lesser
R1459 T9584 T9585 det the,production
R1460 T9585 T9583 pobj production,than
R1461 T9586 T9585 amod increased,production
R1462 T9460 T9445 punct ", ",concentrate
R1463 T9587 T9585 compound urine,production
R1464 T9588 T9585 cc and,production
R1465 T9589 T9590 compound water,intake
R1466 T9461 T9445 prep from,concentrate
R1467 T9590 T9585 conj intake,production
R1468 T9591 T9582 punct ", ",was
R1469 T9592 T9582 expl there,was
R1470 T9462 T9461 pobj 161,from
R1471 T9593 T9594 det no,phenotype
R1472 T9594 T9582 attr phenotype,was
R1473 T9463 T9461 prep to,from
R1474 T9595 T9594 amod overt,phenotype
R1475 T9596 T9594 prep in,phenotype
R1476 T9597 T9598 compound mutant,mice
R1477 T9598 T9596 pobj mice,in
R1478 T9599 T9594 punct ", ",phenotype
R1479 T9600 T9594 prep save,phenotype
R1480 T9464 T9463 pobj 470,to
R1481 T9601 T9602 amod distended,kidneys
R1482 T9602 T9600 pobj kidneys,save
R1483 T9603 T9602 punct ", ",kidneys
R1484 T9465 T9461 pobj mOsm,from
R1485 T9604 T9605 dep which,appeared
R1486 T9605 T9602 relcl appeared,kidneys
R1487 T9606 T9605 advmod variably,appeared
R1488 T9466 T9467 punct (,2.9-fold
R1489 T9607 T9605 prep in,appeared
R1490 T9608 T9609 amod adult,animals
R1491 T9609 T9607 pobj animals,in
R1492 T9467 T9461 parataxis 2.9-fold,from
R1493 T9610 T9611 punct (,Figure
R1494 T9611 T9582 parataxis Figure,was
R1495 T9612 T9611 nummod 2A,Figure
R1496 T9468 T9467 punct ),2.9-fold
R1497 T9613 T9611 punct ),Figure
R1498 T9614 T9582 punct .,was
R1499 T9469 T9445 punct ", ",concentrate
R1500 T9470 T9445 advcl indicating,concentrate
R1501 T9471 T9472 mark that,are
R1502 T9616 T9617 mark Although,measured
R1503 T9472 T9470 ccomp are,indicating
R1504 T9617 T9620 advcl measured,seem
R1505 T9618 T9617 neg not,measured
R1506 T9619 T9617 advmod specifically,measured
R1507 T9473 T9474 det these,animals
R1508 T9621 T9620 punct ", ",seem
R1509 T9622 T9623 compound mutant,mice
R1510 T9474 T9472 nsubj animals,are
R1511 T9623 T9620 nsubj mice,seem
R1512 T9624 T9625 aux to,have
R1513 T9625 T9620 xcomp have,seem
R1514 T9475 T9472 preconj not,are
R1515 T9626 T9627 det a,lifespan
R1516 T9627 T9625 dobj lifespan,have
R1517 T9628 T9627 amod normal,lifespan
R1518 T9476 T9475 advmod only,not
R1519 T9629 T9620 punct .,seem
R1520 T9477 T9472 conj unable,are
R1521 T9631 T9632 det The,animal
R1522 T9632 T9634 nsubj animal,lived
R1523 T9633 T9632 nummod one,animal
R1524 T9478 T9479 aux to,concentrate
R1525 T9635 T9636 dep that,followed
R1526 T9636 T9632 relcl followed,animal
R1527 T9637 T9636 auxpass was,followed
R1528 T9638 T9634 prep to,lived
R1529 T9639 T9640 nummod 18,mo
R1530 T9479 T9477 xcomp concentrate,unable
R1531 T9640 T9638 pobj mo,to
R1532 T9641 T9634 punct ", ",lived
R1533 T9642 T9634 advcl typical,lived
R1534 T9480 T9481 poss their,urine
R1535 T9643 T9642 prep for,typical
R1536 T9644 T9643 pobj animals,for
R1537 T9645 T9644 prep in,animals
R1538 T9481 T9479 dobj urine,concentrate
R1539 T9646 T9647 poss our,colony
R1540 T9647 T9645 pobj colony,in
R1541 T9648 T9634 punct .,lived
R1542 T9482 T9479 advmod properly,concentrate
R1543 T9650 T9651 compound Aqp2F204V,F204V
R1544 T9651 T9653 compound F204V,mice
R1545 T9483 T9472 cc but,are
R1546 T9652 T9651 punct /,F204V
R1547 T9653 T9654 nsubj mice,suffer
R1548 T9484 T9472 conj are,are
R1549 T9655 T9654 prep from,suffer
R1550 T9656 T9657 amod severe,hydronephrosis
R1551 T9485 T9484 advmod also,are
R1552 T9657 T9655 pobj hydronephrosis,from
R1553 T9658 T9659 punct (,2A
R1554 T9486 T9484 acomp defective,are
R1555 T9659 T9654 parataxis 2A,suffer
R1556 T9660 T9659 nmod Figure,2A
R1557 T9661 T9659 cc and,2A
R1558 T9487 T9484 prep in,are
R1559 T9662 T9659 conj 2B,2A
R1560 T9663 T9659 punct ),2A
R1561 T9664 T9654 punct ", ",suffer
R1562 T9488 T9489 poss their,response
R1563 T9665 T9666 advmod presumably,as
R1564 T9666 T9654 prep as,suffer
R1565 T9667 T9668 det a,consequence
R1566 T9489 T9487 pobj response,in
R1567 T9668 T9666 pobj consequence,as
R1568 T9669 T9668 prep of,consequence
R1569 T9490 T9489 prep to,response
R1570 T9491 T9490 pobj dDAVP,to
R1571 T9670 T9671 det an,inability
R1572 T9492 T9445 punct .,concentrate
R1573 T9671 T9669 pobj inability,of
R1574 T9672 T9673 aux to,cope
R1575 T9673 T9671 acl cope,inability
R1576 T9494 T9495 det The,response
R1577 T9674 T9673 prep with,cope
R1578 T9675 T9676 det the,polyuria
R1579 T9676 T9674 pobj polyuria,with
R1580 T9677 T9676 amod extreme,polyuria
R1581 T9678 T9654 punct .,suffer
R1582 T9495 T9497 nsubj response,indicates
R1583 T9680 T9681 nsubj We,found
R1584 T9681 T9682 ccomp found,was
R1585 T9496 T9495 amod smaller,response
R1586 T9683 T9684 amod distended,kidneys
R1587 T9684 T9681 dobj kidneys,found
R1588 T9685 T9681 prep in,found
R1589 T9498 T9495 prep to,response
R1590 T9686 T9687 det all,mice
R1591 T9687 T9685 pobj mice,in
R1592 T9688 T9689 compound Aqp2F204V,F204V
R1593 T9689 T9687 compound F204V,mice
R1594 T9499 T9498 pobj dDAVP,to
R1595 T9690 T9689 punct /,F204V
R1596 T9691 T9682 punct ;,was
R1597 T9692 T9682 advmod however,was
R1598 T9500 T9501 det some,activity
R1599 T9693 T9682 punct ", ",was
R1600 T9694 T9695 det the,degree
R1601 T9695 T9682 nsubj degree,was
R1602 T9501 T9497 dobj activity,indicates
R1603 T9696 T9695 prep of,degree
R1604 T9697 T9696 pobj inflation,of
R1605 T9502 T9501 amod residual,activity
R1606 T9698 T9682 acomp variable,was
R1607 T9699 T9682 prep in,was
R1608 T9700 T9701 amod affected,mice
R1609 T9503 T9501 prep of,activity
R1610 T9701 T9699 pobj mice,in
R1611 T9702 T9682 cc and,was
R1612 T9703 T9682 conj worsened,was
R1613 T9704 T9703 prep with,worsened
R1614 T9504 T9505 det the,channel
R1615 T9705 T9704 pobj age,with
R1616 T9706 T9682 punct .,was
R1617 T9505 T9503 pobj channel,of
R1618 T9708 T9709 amod Severe,hydronephrosis
R1619 T9709 T9710 nsubjpass hydronephrosis,observed
R1620 T9506 T9505 compound mutant,channel
R1621 T9711 T9710 aux has,observed
R1622 T9712 T9710 advmod previously,observed
R1623 T9507 T9505 compound AQP2,channel
R1624 T9713 T9710 auxpass been,observed
R1625 T9714 T9710 prep in,observed
R1626 T9715 T9716 amod double,knock
R1627 T9508 T9505 punct ", ",channel
R1628 T9716 T9720 amod knock,mice
R1629 T9717 T9718 compound Aqp1,Aqp3
R1630 T9718 T9716 npadvmod Aqp3,knock
R1631 T9719 T9718 punct /,Aqp3
R1632 T9720 T9714 pobj mice,in
R1633 T9827 T9828 det the,features
R1634 T9721 T9716 punct -,knock
R1635 T9828 T9825 nsubj features,were
R1636 T9722 T9716 prt out,knock
R1637 T9723 T9724 punct [,17
R1638 T9829 T9828 amod morphologic,features
R1639 T9724 T9710 parataxis 17,observed
R1640 T9725 T9724 punct ],17
R1641 T9726 T9710 punct ", ",observed
R1642 T9830 T9828 prep of,features
R1643 T9727 T9710 cc and,observed
R1644 T9728 T9710 conj appears,observed
R1645 T9729 T9728 prep at,appears
R1646 T9831 T9832 det the,glomeruli
R1647 T9730 T9731 nummod 6,wk
R1648 T9731 T9729 pobj wk,at
R1649 T9732 T9710 punct .,observed
R1650 T9832 T9830 pobj glomeruli,of
R1651 T9734 T9735 advmod Even,at
R1652 T9833 T9832 cc and,glomeruli
R1653 T9735 T9736 prep at,had
R1654 T9737 T9738 nummod 4,wk
R1655 T9834 T9835 amod proximal,distal
R1656 T9738 T9735 pobj wk,at
R1657 T9739 T9736 punct ", ",had
R1658 T9835 T9837 amod distal,tubules
R1659 T9740 T9741 compound Aqp2F204V,F204V
R1660 T9741 T9743 compound F204V,mice
R1661 T9742 T9741 punct /,F204V
R1662 T9743 T9736 nsubj mice,had
R1663 T9836 T9835 punct /,distal
R1664 T9744 T9736 dobj hydronephrosis,had
R1665 T9745 T9736 punct .,had
R1666 T9837 T9832 conj tubules,glomeruli
R1667 T9747 T9748 amod Histologic,sections
R1668 T9748 T9749 nsubj sections,demonstrated
R1669 T9838 T9825 acomp unremarkable,were
R1670 T9750 T9748 prep from,sections
R1671 T9751 T9752 compound Aqp2F204V,F204V
R1672 T9839 T9840 punct (,Figure
R1673 T9752 T9754 compound F204V,mice
R1674 T9753 T9752 punct /,F204V
R1675 T9754 T9750 pobj mice,from
R1676 T9840 T9825 parataxis Figure,were
R1677 T9755 T9756 amod marked,dilatation
R1678 T9756 T9749 dobj dilatation,demonstrated
R1679 T9841 T9840 nummod 2D,Figure
R1680 T9757 T9756 prep of,dilatation
R1681 T9758 T9759 det the,pelvis
R1682 T9842 T9840 punct ),Figure
R1683 T9759 T9757 pobj pelvis,of
R1684 T9760 T9759 amod renal,pelvis
R1685 T9761 T9756 cc yet,dilatation
R1686 T9762 T9763 amod normal,morphology
R1687 T9843 T9825 punct .,were
R1688 T9763 T9756 conj morphology,dilatation
R1689 T9764 T9763 prep of,morphology
R1690 T9765 T9766 det the,ureter
R1691 T9845 T9846 mark As,shown
R1692 T9766 T9764 pobj ureter,of
R1693 T9767 T9768 punct (,2C
R1694 T9768 T9749 parataxis 2C,demonstrated
R1695 T9846 T9847 advcl shown,revealed
R1696 T9769 T9768 nmod Figure,2C
R1697 T9770 T9768 cc and,2C
R1698 T9771 T9768 conj 2D,2C
R1699 T9848 T9846 advmod previously,shown
R1700 T9772 T9768 punct ),2C
R1701 T9773 T9749 punct .,demonstrated
R1702 T9849 T9850 punct [,22
R1703 T9775 T9776 prep In,was
R1704 T9777 T9775 amod particular,In
R1705 T9778 T9776 punct ", ",was
R1706 T9779 T9780 det the,propria
R1707 T9850 T9846 parataxis 22,shown
R1708 T9780 T9776 nsubj propria,was
R1709 T9781 T9780 compound muscularis,propria
R1710 T9782 T9783 preconj neither,hypertrophied
R1711 T9851 T9850 nummod 18,22
R1712 T9852 T9850 punct ",",22
R1713 T9783 T9776 acomp hypertrophied,was
R1714 T9853 T9850 punct ],22
R1715 T9784 T9783 cc nor,hypertrophied
R1716 T9785 T9783 conj thinned,hypertrophied
R1717 T9854 T9847 punct ", ",revealed
R1718 T9786 T9776 punct .,was
R1719 T9855 T9847 nsubj immunoblotting,revealed
R1720 T9788 T9789 expl There,was
R1721 T9790 T9791 det the,appearance
R1722 T9856 T9857 nummod three,forms
R1723 T9791 T9789 attr appearance,was
R1724 T9792 T9791 amod normal,appearance
R1725 T9857 T9847 dobj forms,revealed
R1726 T9793 T9791 amod festooned,appearance
R1727 T9794 T9791 prep of,appearance
R1728 T9795 T9796 det the,urothelium
R1729 T9796 T9794 pobj urothelium,of
R1730 T9858 T9857 amod different,forms
R1731 T9797 T9789 punct ", ",was
R1732 T9798 T9789 cc and,was
R1733 T9859 T9857 prep of,forms
R1734 T9799 T9800 det this,epithelium
R1735 T9800 T9802 nsubj epithelium,was
R1736 T9801 T9800 amod transitional,epithelium
R1737 T9802 T9789 conj was,was
R1738 T9803 T9802 prep of,was
R1739 T9804 T9805 amod normal,thickness
R1740 T9860 T9859 pobj AQP2,of
R1741 T9805 T9803 pobj thickness,of
R1742 T9806 T9802 punct .,was
R1743 T9861 T9847 punct ", ",revealed
R1744 T9808 T9809 expl There,was
R1745 T9810 T9809 attr thinning,was
R1746 T9862 T9847 prep due,revealed
R1747 T9811 T9810 prep of,thinning
R1748 T9812 T9813 det the,kidney
R1749 T9813 T9811 pobj kidney,of
R1750 T9863 T9862 pcomp to,due
R1751 T9814 T9815 mark as,measured
R1752 T9815 T9809 advcl measured,was
R1753 T9816 T9815 prep from,measured
R1754 T9864 T9865 amod different,degrees
R1755 T9817 T9818 amod renal,capsule
R1756 T9818 T9816 pobj capsule,from
R1757 T9819 T9816 prep to,from
R1758 T9865 T9862 pobj degrees,due
R1759 T9820 T9821 amod renal,pelvis
R1760 T9821 T9819 pobj pelvis,to
R1761 T9866 T9865 cc and,degrees
R1762 T9822 T9809 punct .,was
R1763 T9824 T9825 advmod However,were
R1764 T9867 T9865 conj forms,degrees
R1765 T9826 T9825 punct ", ",were
R1766 T9868 T9865 prep of,degrees
R1767 T9869 T9868 pobj glycosylation,of
R1768 T9870 T9871 punct (,Figure
R1769 T9871 T9847 parataxis Figure,revealed
R1770 T9872 T9871 nummod 3A,Figure
R1771 T9933 T9932 punct ],22
R1772 T9873 T9871 punct ),Figure
R1773 T9934 T9911 punct .,is
R1774 T9874 T9847 punct .,revealed
R1775 T9936 T9937 prep Compared,reduced
R1776 T9876 T9877 amod Previous,reports
R1777 T9938 T9936 prep to,Compared
R1778 T9939 T9938 pobj that,to
R1779 T9940 T9939 prep from,that
R1780 T9941 T9942 det the,kidneys
R1781 T9877 T9878 nsubj reports,demonstrated
R1782 T9942 T9940 pobj kidneys,from
R1783 T9943 T9942 prep of,kidneys
R1784 T9879 T9878 aux have,demonstrated
R1785 T9944 T9945 amod wild,type
R1786 T9945 T9947 compound type,animals
R1787 T9946 T9945 punct -,type
R1788 T9947 T9943 pobj animals,of
R1789 T9948 T9937 punct ", ",reduced
R1790 T9949 T9937 nsubjpass AQP2,reduced
R1791 T9880 T9881 mark that,appears
R1792 T9950 T9949 prep from,AQP2
R1793 T9951 T9952 compound mutant,animals
R1794 T9952 T9950 pobj animals,from
R1795 T9881 T9878 ccomp appears,demonstrated
R1796 T9953 T9937 auxpass was,reduced
R1797 T9954 T9937 prep in,reduced
R1798 T9955 T9956 preconj both,form
R1799 T9882 T9883 amod nonglycosylated,protein
R1800 T9956 T9954 pobj form,in
R1801 T9957 T9956 det the,form
R1802 T9883 T9881 nsubj protein,appears
R1803 T9958 T9959 amod high,weight
R1804 T9959 T9956 nmod weight,form
R1805 T9960 T9959 amod molecular,weight
R1806 T9884 T9881 prep as,appears
R1807 T9961 T9956 punct ", ",form
R1808 T9962 T9956 amod diffuse,form
R1809 T9963 T9956 cc and,form
R1810 T9885 T9886 det a,band
R1811 T9964 T9965 det the,form
R1812 T9965 T9956 conj form,form
R1813 T9966 T9967 amod lowest,weight
R1814 T9886 T9884 pobj band,as
R1815 T9967 T9965 compound weight,form
R1816 T9968 T9967 amod molecular,weight
R1817 T9969 T9937 punct ", ",reduced
R1818 T9887 T9888 nummod 29,kDa
R1819 T9970 T9937 cc but,reduced
R1820 T9971 T9937 conj enriched,reduced
R1821 T9888 T9886 compound kDa,band
R1822 T9972 T9971 prep in,enriched
R1823 T9973 T9974 det the,form
R1824 T9974 T9972 pobj form,in
R1825 T9889 T9881 punct ", ",appears
R1826 T9975 T9976 amod intermediate,weight
R1827 T9976 T9974 compound weight,form
R1828 T9977 T9976 amod molecular,weight
R1829 T9890 T9891 mark while,runs
R1830 T9978 T9979 punct (,Figure
R1831 T9979 T9971 parataxis Figure,enriched
R1832 T9980 T9979 nummod 3A,Figure
R1833 T9891 T9881 advcl runs,appears
R1834 T9981 T9979 punct ),Figure
R1835 T9982 T9937 punct .,reduced
R1836 T9892 T9893 amod complex,protein
R1837 T9984 T9985 amod Heterozygous,animals
R1838 T9985 T9986 nsubj animals,showed
R1839 T9987 T9988 amod intermediate,amounts
R1840 T9988 T9986 dobj amounts,showed
R1841 T9893 T9891 nsubj protein,runs
R1842 T9989 T9988 prep of,amounts
R1843 T9990 T9991 det all,forms
R1844 T9991 T9989 pobj forms,of
R1845 T9894 T9893 amod glycosylated,protein
R1846 T9992 T9991 nummod three,forms
R1847 T9993 T9986 punct .,showed
R1848 T9895 T9891 prep as,runs
R1849 T9995 T9996 det The,nature
R1850 T9996 T9997 nsubjpass nature,revealed
R1851 T9896 T9897 det a,smear
R1852 T9998 T9996 prep of,nature
R1853 T9999 T10000 det these,forms
R1854 T9897 T9895 pobj smear,as
R1855 T10000 T9998 pobj forms,of
R1856 T10001 T10000 amod glycosylated,forms
R1857 T9898 T9897 prep between,smear
R1858 T10002 T9997 auxpass was,revealed
R1859 T10003 T9997 prep by,revealed
R1860 T10004 T10003 pobj digestion,by
R1861 T9899 T9900 nummod 35,kDa
R1862 T10005 T10004 prep with,digestion
R1863 T10006 T10007 compound endoglycosidase,H
R1864 T10007 T10005 pobj H,with
R1865 T9900 T9898 pobj kDa,between
R1866 T10008 T10007 punct ", ",H
R1867 T10009 T10010 dep which,cleaves
R1868 T9901 T9899 cc and,35
R1869 T10010 T10007 relcl cleaves,H
R1870 T10011 T10010 advmod specifically,cleaves
R1871 T10012 T10013 npadvmod mannose,rich
R1872 T9902 T9899 conj 45,35
R1873 T10013 T10015 amod rich,carbohydrate
R1874 T10014 T10013 punct -,rich
R1875 T10015 T10010 dobj carbohydrate,cleaves
R1876 T9903 T9878 punct .,demonstrated
R1877 T10016 T10010 prep from,cleaves
R1878 T10017 T10018 det the,backbone
R1879 T10018 T10016 pobj backbone,from
R1880 T9905 T9906 det A,form
R1881 T10019 T10018 compound protein,backbone
R1882 T9906 T9911 nsubj form,is
R1883 T10020 T9997 punct .,revealed
R1884 T9907 T9908 amod short,lived
R1885 T10022 T10023 nsubj Treatment,affected
R1886 T9908 T9906 amod lived,form
R1887 T10024 T10022 prep of,Treatment
R1888 T10025 T10026 amod endogenous,AQP2
R1889 T10026 T10024 pobj AQP2,of
R1890 T9909 T9908 punct -,lived
R1891 T10027 T10026 prep from,AQP2
R1892 T10028 T10027 pobj kidneys,from
R1893 T10029 T10028 prep of,kidneys
R1894 T10030 T10031 amod wild,type
R1895 T10031 T10033 nmod type,animals
R1896 T9910 T9906 amod intermediate,form
R1897 T10032 T10031 punct -,type
R1898 T10033 T10029 pobj animals,of
R1899 T9912 T9906 prep of,form
R1900 T10034 T10031 punct ", ",type
R1901 T10035 T10031 conj heterozygous,type
R1902 T10036 T10035 punct ", ",heterozygous
R1903 T10037 T10035 cc and,heterozygous
R1904 T9913 T9914 nummod 31,kDa
R1905 T10038 T10035 conj mutant,heterozygous
R1906 T9914 T9912 pobj kDa,of
R1907 T9915 T9906 acl representing,form
R1908 T9916 T9917 nmod core,glycosylation
R1909 T10039 T10023 advmod specifically,affected
R1910 T10040 T10041 det the,form
R1911 T9917 T9915 dobj glycosylation,representing
R1912 T10041 T10023 dobj form,affected
R1913 T10042 T10043 amod intermediate,weight
R1914 T10043 T10041 compound weight,form
R1915 T9918 T9917 punct ", ",glycosylation
R1916 T10044 T10043 amod molecular,weight
R1917 T10045 T10046 punct (,Figure
R1918 T9919 T9920 amod high,mannose
R1919 T10046 T10023 parataxis Figure,affected
R1920 T10047 T10046 nummod 3B,Figure
R1921 T10048 T10046 punct ),Figure
R1922 T9920 T9917 compound mannose,glycosylation
R1923 T10049 T10023 punct .,affected
R1924 T10051 T10052 det The,presence
R1925 T9921 T9920 punct -,mannose
R1926 T10052 T10053 nsubj presence,permits
R1927 T9922 T9917 prep of,glycosylation
R1928 T10054 T10052 prep of,presence
R1929 T10055 T10056 det some,proteins
R1930 T10056 T10054 pobj proteins,of
R1931 T9923 T9922 pobj AQP2,of
R1932 T10057 T10056 amod mature,proteins
R1933 T10058 T10056 amod glycosylated,proteins
R1934 T10059 T10060 punct (,kDa
R1935 T9924 T9911 acomp apparent,is
R1936 T10060 T10056 parataxis kDa,proteins
R1937 T10061 T10062 quantmod 35,45
R1938 T10062 T10060 nummod 45,kDa
R1939 T9925 T9924 prep from,apparent
R1940 T10063 T10062 punct –,45
R1941 T10064 T10060 punct ),kDa
R1942 T9926 T9927 compound pulse,chase
R1943 T10065 T10056 prep in,proteins
R1944 T10066 T10067 compound Aqp2F204V,F204V
R1945 T10067 T10069 compound F204V,mice
R1946 T10068 T10067 punct /,F204V
R1947 T10069 T10065 pobj mice,in
R1948 T10070 T10053 advmod presumably,permits
R1949 T9927 T9929 compound chase,labeling
R1950 T10071 T10072 poss their,survival
R1951 T10072 T10053 dobj survival,permits
R1952 T10073 T10072 prep compared,survival
R1953 T9928 T9927 punct -,chase
R1954 T10074 T10073 prep to,compared
R1955 T10075 T10076 compound Aqp2T126M,T126M
R1956 T10076 T10078 compound T126M,mice
R1957 T9929 T9930 compound labeling,experiments
R1958 T10077 T10076 punct /,T126M
R1959 T10078 T10074 pobj mice,to
R1960 T10079 T10053 punct ", ",permits
R1961 T9930 T9925 pobj experiments,from
R1962 T10080 T10053 cc and,permits
R1963 T10081 T10053 conj is,permits
R1964 T10082 T10081 acomp consistent,is
R1965 T9931 T9932 punct [,22
R1966 T10083 T10082 prep with,consistent
R1967 T10084 T10085 det a,response
R1968 T9932 T9911 parataxis 22,is
R1969 T10085 T10083 pobj response,with
R1970 T10086 T10085 amod diminished,response
R1971 T10087 T10085 prep to,response
R1972 T10088 T10087 pobj dDAVP,to
R1973 T10146 T10147 prep In,fail
R1974 T10089 T10053 punct .,permits
R1975 T10091 T10092 prep In,postulated
R1976 T10093 T10091 pobj humans,In
R1977 T10148 T10146 amod general,In
R1978 T10094 T10092 punct ", ",postulated
R1979 T10095 T10096 amod recessive,alleles
R1980 T10096 T10092 nsubjpass alleles,postulated
R1981 T10097 T10096 prep of,alleles
R1982 T10149 T10147 punct ", ",fail
R1983 T10098 T10097 pobj Aqp2,of
R1984 T10099 T10092 auxpass are,postulated
R1985 T10100 T10101 aux to,cause
R1986 T10150 T10151 amod such,alleles
R1987 T10101 T10092 xcomp cause,postulated
R1988 T10102 T10101 dobj NDI,cause
R1989 T10103 T10104 mark because,translocate
R1990 T10151 T10147 nsubj alleles,fail
R1991 T10152 T10151 amod recessive,alleles
R1992 T10104 T10101 advcl translocate,cause
R1993 T10105 T10104 nsubj they,translocate
R1994 T10153 T10147 punct ", ",fail
R1995 T10106 T10104 aux do,translocate
R1996 T10107 T10104 neg not,translocate
R1997 T10108 T10104 advmod properly,translocate
R1998 T10109 T10104 prep to,translocate
R1999 T10110 T10111 det the,surface
R2000 T10154 T10155 advmod when,visualized
R2001 T10111 T10109 pobj surface,to
R2002 T10112 T10111 amod apical,surface
R2003 T10113 T10111 compound cell,surface
R2004 T10114 T10104 prep in,translocate
R2005 T10155 T10147 advcl visualized,fail
R2006 T10115 T10114 pobj response,in
R2007 T10116 T10115 prep to,response
R2008 T10156 T10155 advmod immunocytochemically,visualized
R2009 T10117 T10116 pobj AVP,to
R2010 T10118 T10092 punct .,postulated
R2011 T10157 T10147 punct ", ",fail
R2012 T10120 T10121 det This,postulate
R2013 T10121 T10122 nsubj postulate,comes
R2014 T10158 T10159 aux to,localize
R2015 T10123 T10124 advmod solely,from
R2016 T10124 T10122 prep from,comes
R2017 T10159 T10147 xcomp localize,fail
R2018 T10125 T10126 advmod in,vitro
R2019 T10126 T10127 amod vitro,studies
R2020 T10127 T10124 pobj studies,from
R2021 T10160 T10159 prep to,localize
R2022 T10128 T10129 prep in,transfected
R2023 T10129 T10127 relcl transfected,studies
R2024 T10130 T10128 pobj which,in
R2025 T10161 T10162 npadvmod AVP,responsive
R2026 T10131 T10132 compound mutant,cDNAs
R2027 T10132 T10129 nsubjpass cDNAs,transfected
R2028 T10133 T10132 compound Aqp2,cDNAs
R2029 T10162 T10164 amod responsive,vesicles
R2030 T10134 T10132 acl corresponding,cDNAs
R2031 T10135 T10134 prep to,corresponding
R2032 T10136 T10137 amod human,mutations
R2033 T10163 T10162 punct -,responsive
R2034 T10137 T10135 pobj mutations,to
R2035 T10138 T10137 compound disease,mutations
R2036 T10164 T10160 pobj vesicles,to
R2037 T10139 T10129 auxpass are,transfected
R2038 T10140 T10129 prep into,transfected
R2039 T10141 T10142 compound kidney,lines
R2040 T10165 T10147 punct .,fail
R2041 T10142 T10140 pobj lines,into
R2042 T10143 T10142 compound cell,lines
R2043 T10144 T10122 punct .,comes
R2044 T10167 T10168 advmod Rather,trapped
R2045 T10169 T10168 punct ", ",trapped
R2046 T10170 T10168 nsubjpass they,trapped
R2047 T10171 T10168 auxpass get,trapped
R2048 T10172 T10168 prep in,trapped
R2049 T10250 T10237 parataxis row,translocated
R2050 T10251 T10250 dep Figure,row
R2051 T10173 T10174 det the,reticulum
R2052 T10252 T10251 nummod 4A,Figure
R2053 T10253 T10250 punct ", ",row
R2054 T10174 T10172 pobj reticulum,in
R2055 T10254 T10250 amod second,row
R2056 T10255 T10250 punct ),row
R2057 T10256 T10237 punct .,translocated
R2058 T10175 T10174 amod endoplasmic,reticulum
R2059 T10258 T10259 prep In,distributed
R2060 T10176 T10174 punct (,reticulum
R2061 T10260 T10258 pobj kidneys,In
R2062 T10261 T10260 acl taken,kidneys
R2063 T10177 T10174 appos ER,reticulum
R2064 T10262 T10261 prep from,taken
R2065 T10263 T10264 compound mutant,animals
R2066 T10264 T10262 pobj animals,from
R2067 T10178 T10168 punct ),trapped
R2068 T10265 T10259 punct ", ",distributed
R2069 T10266 T10259 advmod however,distributed
R2070 T10267 T10259 punct ", ",distributed
R2071 T10179 T10168 punct .,trapped
R2072 T10268 T10259 nsubjpass AQP2,distributed
R2073 T10269 T10259 auxpass was,distributed
R2074 T10270 T10259 advmod randomly,distributed
R2075 T10271 T10259 prep throughout,distributed
R2076 T10181 T10182 poss Our,model
R2077 T10272 T10273 det the,cell
R2078 T10273 T10271 pobj cell,throughout
R2079 T10274 T10259 prep in,distributed
R2080 T10275 T10276 det the,state
R2081 T10182 T10184 nsubj model,affords
R2082 T10276 T10274 pobj state,in
R2083 T10277 T10276 amod basal,state
R2084 T10278 T10279 punct (,row
R2085 T10183 T10182 compound mouse,model
R2086 T10185 T10182 prep of,model
R2087 T10279 T10259 parataxis row,distributed
R2088 T10186 T10185 pobj NDI,of
R2089 T10280 T10279 dep Figure,row
R2090 T10281 T10280 nummod 4A,Figure
R2091 T10187 T10188 det the,opportunity
R2092 T10282 T10279 punct ", ",row
R2093 T10283 T10279 amod third,row
R2094 T10284 T10279 punct ),row
R2095 T10188 T10184 dobj opportunity,affords
R2096 T10285 T10259 punct ", ",distributed
R2097 T10286 T10287 mark while,localized
R2098 T10189 T10188 amod first,opportunity
R2099 T10287 T10259 advcl localized,distributed
R2100 T10288 T10287 nsubj AQP3,localized
R2101 T10289 T10290 punct (,green
R2102 T10290 T10288 parataxis green,AQP3
R2103 T10291 T10290 punct ),green
R2104 T10190 T10191 aux to,test
R2105 T10292 T10287 advmod appropriately,localized
R2106 T10293 T10287 prep to,localized
R2107 T10294 T10295 det the,surface
R2108 T10191 T10188 acl test,opportunity
R2109 T10295 T10293 pobj surface,to
R2110 T10296 T10295 amod basolateral,surface
R2111 T10297 T10298 punct [,23
R2112 T10192 T10193 det this,hypothesis
R2113 T10298 T10287 parataxis 23,localized
R2114 T10299 T10298 punct ],23
R2115 T10300 T10259 punct .,distributed
R2116 T10193 T10191 dobj hypothesis,test
R2117 T10302 T10303 advmod Furthermore,failed
R2118 T10194 T10191 prep in,test
R2119 T10304 T10303 punct ", ",failed
R2120 T10305 T10303 prep upon,failed
R2121 T10306 T10307 compound dDAVP,stimulation
R2122 T10195 T10196 det a,animal
R2123 T10307 T10305 pobj stimulation,upon
R2124 T10308 T10303 punct ", ",failed
R2125 T10309 T10310 compound AQP2,F204V
R2126 T10196 T10194 pobj animal,in
R2127 T10310 T10303 nsubj F204V,failed
R2128 T10311 T10310 punct -,F204V
R2129 T10312 T10313 aux to,translocate
R2130 T10197 T10196 amod mature,animal
R2131 T10313 T10303 xcomp translocate,failed
R2132 T10314 T10313 prep to,translocate
R2133 T10198 T10184 punct .,affords
R2134 T10315 T10316 det the,surface
R2135 T10316 T10314 pobj surface,to
R2136 T10317 T10316 compound cell,surface
R2137 T10200 T10201 mark As,shown
R2138 T10318 T10319 punct (,row
R2139 T10319 T10303 parataxis row,failed
R2140 T10320 T10319 dep Figure,row
R2141 T10321 T10320 nummod 4A,Figure
R2142 T10322 T10319 punct ", ",row
R2143 T10201 T10202 advcl shown,localized
R2144 T10323 T10319 amod bottom,row
R2145 T10324 T10319 punct ),row
R2146 T10203 T10201 prep in,shown
R2147 T10325 T10303 punct .,failed
R2148 T10327 T10328 aux To,confirm
R2149 T10328 T10329 advcl confirm,repeated
R2150 T10204 T10203 pobj Figure,in
R2151 T10330 T10331 det these,findings
R2152 T10331 T10328 dobj findings,confirm
R2153 T10332 T10329 punct ", ",repeated
R2154 T10333 T10334 det the,staining
R2155 T10334 T10329 nsubjpass staining,repeated
R2156 T10205 T10204 nummod 4A,Figure
R2157 T10335 T10329 auxpass was,repeated
R2158 T10336 T10329 prep in,repeated
R2159 T10337 T10336 pobj kidneys,in
R2160 T10206 T10207 punct (,row
R2161 T10338 T10337 acl taken,kidneys
R2162 T10339 T10338 prep from,taken
R2163 T10340 T10341 nummod two,mice
R2164 T10341 T10339 pobj mice,from
R2165 T10207 T10204 parataxis row,Figure
R2166 T10342 T10341 amod further,mice
R2167 T10343 T10338 prep for,taken
R2168 T10208 T10207 amod top,row
R2169 T10344 T10345 det each,class
R2170 T10345 T10343 pobj class,for
R2171 T10209 T10207 prep of,row
R2172 T10346 T10345 punct ", ",class
R2173 T10347 T10348 amod wild,type
R2174 T10348 T10345 appos type,class
R2175 T10210 T10209 pobj photomicrographs,of
R2176 T10349 T10348 punct -,type
R2177 T10350 T10348 cc or,type
R2178 T10351 T10348 conj mutant,type
R2179 T10211 T10207 punct ),row
R2180 T10352 T10329 punct ", ",repeated
R2181 T10353 T10329 prep with,repeated
R2182 T10354 T10353 cc or,with
R2183 T10212 T10202 punct ", ",localized
R2184 T10355 T10353 conj without,with
R2185 T10213 T10202 nsubj AQP2,localized
R2186 T10214 T10213 punct (,AQP2
R2187 T10215 T10213 acl stained,AQP2
R2188 T10356 T10357 compound dDAVP,treatment
R2189 T10357 T10355 pobj treatment,without
R2190 T10216 T10215 oprd red,stained
R2191 T10358 T10329 punct ", ",repeated
R2192 T10359 T10329 prep with,repeated
R2193 T10217 T10213 punct ),AQP2
R2194 T10360 T10361 amod identical,results
R2195 T10361 T10359 pobj results,with
R2196 T10362 T10329 punct .,repeated
R2197 T10218 T10202 advmod normally,localized
R2198 T10364 T10365 aux To,investigate
R2199 T10365 T10366 advcl investigate,turned
R2200 T10219 T10202 prep to,localized
R2201 T10367 T10368 det the,mechanism
R2202 T10220 T10221 det the,region
R2203 T10368 T10365 dobj mechanism,investigate
R2204 T10221 T10219 pobj region,to
R2205 T10369 T10368 prep of,mechanism
R2206 T10370 T10371 amod defective,translocation
R2207 T10222 T10221 amod subapical,region
R2208 T10371 T10369 pobj translocation,of
R2209 T10372 T10371 prep of,translocation
R2210 T10223 T10221 prep of,region
R2211 T10373 T10374 compound AQP2,F204V
R2212 T10374 T10372 pobj F204V,of
R2213 T10375 T10374 punct -,F204V
R2214 T10224 T10225 amod collecting,duct
R2215 T10376 T10366 punct ", ",turned
R2216 T10377 T10366 nsubj we,turned
R2217 T10225 T10226 compound duct,cells
R2218 T10378 T10366 prep to,turned
R2219 T10379 T10378 pobj transfection,to
R2220 T10226 T10223 pobj cells,of
R2221 T10380 T10379 prep of,transfection
R2222 T10381 T10382 compound MDCK,cells
R2223 T10382 T10380 pobj cells,of
R2224 T10227 T10221 prep in,region
R2225 T10383 T10366 punct .,turned
R2226 T10228 T10227 pobj kidneys,in
R2227 T10385 T10386 amod Stable,lines
R2228 T10386 T10388 nsubjpass lines,established
R2229 T10387 T10386 compound cell,lines
R2230 T10229 T10228 prep of,kidneys
R2231 T10389 T10386 acl expressing,lines
R2232 T10390 T10391 nmod mouse,AQP2
R2233 T10230 T10231 amod wild,type
R2234 T10391 T10389 dobj AQP2,expressing
R2235 T10392 T10393 amod wild,type
R2236 T10393 T10391 compound type,AQP2
R2237 T10231 T10233 compound type,mice
R2238 T10394 T10393 punct -,type
R2239 T10395 T10391 cc and,AQP2
R2240 T10232 T10231 punct -,type
R2241 T10396 T10397 compound AQP2,F204V
R2242 T10397 T10391 conj F204V,AQP2
R2243 T10398 T10397 punct -,F204V
R2244 T10233 T10229 pobj mice,of
R2245 T10399 T10388 auxpass were,established
R2246 T10400 T10388 punct .,established
R2247 T10234 T10202 punct .,localized
R2248 T10402 T10403 nsubj Immunoblots,showed
R2249 T10236 T10237 prep Upon,translocated
R2250 T10404 T10402 prep of,Immunoblots
R2251 T10405 T10406 compound protein,extracts
R2252 T10406 T10404 pobj extracts,of
R2253 T10407 T10406 prep from,extracts
R2254 T10408 T10409 amod stable,lines
R2255 T10409 T10407 pobj lines,from
R2256 T10410 T10409 compound cell,lines
R2257 T10238 T10236 pobj stimulation,Upon
R2258 T10411 T10412 mark that,recapitulate
R2259 T10412 T10403 ccomp recapitulate,showed
R2260 T10413 T10414 compound MDCK,cells
R2261 T10414 T10412 nsubj cells,recapitulate
R2262 T10415 T10416 det the,defect
R2263 T10239 T10238 prep with,stimulation
R2264 T10416 T10412 dobj defect,recapitulate
R2265 T10417 T10416 compound glycosylation,defect
R2266 T10240 T10239 pobj dDAVP,with
R2267 T10418 T10416 acl seen,defect
R2268 T10419 T10418 prep in,seen
R2269 T10420 T10421 compound mutant,mice
R2270 T10241 T10237 punct ", ",translocated
R2271 T10421 T10419 pobj mice,in
R2272 T10422 T10423 punct (,data
R2273 T10423 T10403 meta data,showed
R2274 T10424 T10423 amod unpublished,data
R2275 T10242 T10237 nsubj AQP2,translocated
R2276 T10425 T10423 punct ),data
R2277 T10426 T10403 punct .,showed
R2278 T10243 T10237 prep to,translocated
R2279 T10428 T10429 det The,protein
R2280 T10429 T10433 nsubj protein,was
R2281 T10244 T10243 cc or,to
R2282 T10430 T10431 amod wild,type
R2283 T10431 T10429 compound type,protein
R2284 T10432 T10431 punct -,type
R2285 T10245 T10243 conj near,to
R2286 T10434 T10433 advmod again,was
R2287 T10246 T10247 det the,surface
R2288 T10435 T10433 acomp present,was
R2289 T10436 T10433 prep in,was
R2290 T10437 T10438 nummod three,forms
R2291 T10247 T10245 pobj surface,near
R2292 T10438 T10436 pobj forms,in
R2293 T10439 T10438 amod different,forms
R2294 T10248 T10247 compound cell,surface
R2295 T10440 T10433 punct .,was
R2296 T10442 T10443 nsubj Cells,lacked
R2297 T10249 T10250 punct (,row
R2298 T10444 T10442 acl expressing,Cells
R2299 T10445 T10446 compound AQP2,F204V
R2300 T10462 T10461 punct -,glycosylated
R2301 T10446 T10444 dobj F204V,expressing
R2302 T10447 T10446 punct -,F204V
R2303 T10448 T10449 det the,form
R2304 T10449 T10443 dobj form,lacked
R2305 T10463 T10464 nummod 31,kDa
R2306 T10450 T10451 quantmod 35,45
R2307 T10451 T10453 nummod 45,kDa
R2308 T10452 T10451 punct –,45
R2309 T10453 T10449 compound kDa,form
R2310 T10454 T10443 cc and,lacked
R2311 T10455 T10456 auxpass were,enriched
R2312 T10464 T10459 compound kDa,form
R2313 T10456 T10443 conj enriched,lacked
R2314 T10457 T10456 prep in,enriched
R2315 T10465 T10443 punct .,lacked
R2316 T10458 T10459 det the,form
R2317 T10467 T10468 prep In,appeared
R2318 T10459 T10457 pobj form,in
R2319 T10460 T10461 npadvmod core,glycosylated
R2320 T10461 T10459 amod glycosylated,form
R2321 T10469 T10470 amod transfected,cells
R2322 T10470 T10467 pobj cells,In
R2323 T10471 T10470 punct ", ",cells
R2324 T10567 T10568 prep Along,seen
R2325 T10472 T10470 amod unstimulated,cells
R2326 T10569 T10570 det the,axis
R2327 T10570 T10567 pobj axis,Along
R2328 T10473 T10470 compound MDCK,cells
R2329 T10571 T10570 compound z,axis
R2330 T10572 T10570 punct -,axis
R2331 T10573 T10568 punct ", ",seen
R2332 T10474 T10468 punct ", ",appeared
R2333 T10574 T10575 det the,distribution
R2334 T10475 T10476 amod wild,type
R2335 T10476 T10478 compound type,AQP2
R2336 T10575 T10568 nsubjpass distribution,seen
R2337 T10576 T10575 amod perinuclear,distribution
R2338 T10477 T10476 punct -,type
R2339 T10577 T10575 prep of,distribution
R2340 T10578 T10579 compound AQP2,F204V
R2341 T10478 T10468 nsubj AQP2,appeared
R2342 T10579 T10577 pobj F204V,of
R2343 T10580 T10579 punct -,F204V
R2344 T10581 T10568 auxpass was,seen
R2345 T10582 T10568 advmod clearly,seen
R2346 T10479 T10478 punct (,AQP2
R2347 T10583 T10568 punct ", ",seen
R2348 T10584 T10568 cc and,seen
R2349 T10585 T10586 det this,distribution
R2350 T10480 T10478 acl stained,AQP2
R2351 T10586 T10587 nsubjpass distribution,altered
R2352 T10587 T10568 conj altered,seen
R2353 T10588 T10587 auxpass is,altered
R2354 T10481 T10480 oprd green,stained
R2355 T10589 T10587 neg not,altered
R2356 T10590 T10587 agent by,altered
R2357 T10482 T10478 punct ),AQP2
R2358 T10591 T10590 pobj forskolin,by
R2359 T10592 T10593 punct (,columns
R2360 T10593 T10587 parataxis columns,altered
R2361 T10594 T10593 dep Figure,columns
R2362 T10595 T10594 nummod 4B,Figure
R2363 T10596 T10593 punct ", ",columns
R2364 T10483 T10468 prep in,appeared
R2365 T10597 T10598 amod bottom,row
R2366 T10598 T10593 dep row,columns
R2367 T10599 T10593 punct ", ",columns
R2368 T10484 T10485 det a,pattern
R2369 T10600 T10593 nummod two,columns
R2370 T10601 T10593 amod right,columns
R2371 T10602 T10593 punct ),columns
R2372 T10485 T10483 pobj pattern,in
R2373 T10603 T10587 punct .,altered
R2374 T10486 T10485 compound punctate,pattern
R2375 T10605 T10606 det The,distribution
R2376 T10606 T10608 nsubj distribution,is
R2377 T10607 T10606 amod perinuclear,distribution
R2378 T10487 T10485 acl distributed,pattern
R2379 T10609 T10606 prep of,distribution
R2380 T10610 T10611 compound AQP2,F204V
R2381 T10488 T10487 prep throughout,distributed
R2382 T10611 T10609 pobj F204V,of
R2383 T10612 T10611 punct -,F204V
R2384 T10489 T10490 det the,region
R2385 T10613 T10608 acomp consistent,is
R2386 T10614 T10613 prep with,consistent
R2387 T10615 T10616 det an,compartmentalization
R2388 T10616 T10614 pobj compartmentalization,with
R2389 T10490 T10488 pobj region,throughout
R2390 T10617 T10616 compound ER,compartmentalization
R2391 T10618 T10608 punct .,is
R2392 T10491 T10490 amod subapical,region
R2393 T10620 T10621 aux To,test
R2394 T10621 T10622 advcl test,co-stained
R2395 T10492 T10493 punct (,photomicrographs
R2396 T10623 T10624 det the,idea
R2397 T10493 T10468 parataxis photomicrographs,appeared
R2398 T10624 T10621 dobj idea,test
R2399 T10625 T10626 mark that,localizes
R2400 T10626 T10624 acl localizes,idea
R2401 T10494 T10493 dep Figure,photomicrographs
R2402 T10627 T10628 compound AQP2,F204V
R2403 T10628 T10626 nsubj F204V,localizes
R2404 T10629 T10628 punct -,F204V
R2405 T10495 T10494 nummod 4B,Figure
R2406 T10630 T10626 prep to,localizes
R2407 T10631 T10632 det the,ER
R2408 T10496 T10493 punct ", ",photomicrographs
R2409 T10497 T10498 amod left,column
R2410 T10632 T10630 pobj ER,to
R2411 T10633 T10622 punct ", ",co-stained
R2412 T10634 T10622 nsubj we,co-stained
R2413 T10635 T10622 dobj cells,co-stained
R2414 T10636 T10635 acl transfected,cells
R2415 T10498 T10493 compound column,photomicrographs
R2416 T10637 T10636 prep with,transfected
R2417 T10638 T10637 pobj Aqp2F204V,with
R2418 T10499 T10493 punct ),photomicrographs
R2419 T10639 T10640 punct (,cDNA
R2420 T10640 T10638 parataxis cDNA,Aqp2F204V
R2421 T10500 T10468 punct ", ",appeared
R2422 T10641 T10640 punct ),cDNA
R2423 T10642 T10622 prep for,co-stained
R2424 T10643 T10642 pobj AQP2,for
R2425 T10501 T10468 advcl consistent,appeared
R2426 T10644 T10643 cc and,AQP2
R2427 T10645 T10646 det an,marker
R2428 T10646 T10643 conj marker,AQP2
R2429 T10502 T10501 prep with,consistent
R2430 T10647 T10646 compound ER,marker
R2431 T10648 T10646 punct ", ",marker
R2432 T10649 T10646 appos calnexin,marker
R2433 T10503 T10504 amod vesicular,compartmentalization
R2434 T10650 T10651 punct (,Figure
R2435 T10651 T10622 parataxis Figure,co-stained
R2436 T10652 T10651 nummod 4C,Figure
R2437 T10504 T10502 pobj compartmentalization,with
R2438 T10653 T10651 punct ),Figure
R2439 T10654 T10622 punct .,co-stained
R2440 T10505 T10468 punct .,appeared
R2441 T10656 T10657 nsubjpass Colocalization,investigated
R2442 T10658 T10656 prep of,Colocalization
R2443 T10507 T10508 compound AQP2,F204V
R2444 T10659 T10658 pobj calnexin,of
R2445 T10660 T10656 prep with,Colocalization
R2446 T10661 T10660 pobj AQP2,with
R2447 T10662 T10657 auxpass was,investigated
R2448 T10508 T10510 nsubj F204V,appeared
R2449 T10663 T10657 advmod directly,investigated
R2450 T10664 T10657 punct ", ",investigated
R2451 T10665 T10657 cc and,investigated
R2452 T10509 T10508 punct -,F204V
R2453 T10666 T10667 nsubjpass it,found
R2454 T10667 T10657 conj found,investigated
R2455 T10668 T10667 auxpass was,found
R2456 T10511 T10510 punct ", ",appeared
R2457 T10669 T10670 mark that,colocalized
R2458 T10670 T10667 ccomp colocalized,found
R2459 T10671 T10672 nummod 80,%
R2460 T10672 T10670 nsubj %,colocalized
R2461 T10512 T10510 prep on,appeared
R2462 T10513 T10514 det the,hand
R2463 T10673 T10672 prep of,%
R2464 T10514 T10512 pobj hand,on
R2465 T10674 T10675 det all,protein
R2466 T10675 T10673 pobj protein,of
R2467 T10676 T10677 compound AQP2,F204V
R2468 T10515 T10514 amod other,hand
R2469 T10677 T10675 compound F204V,protein
R2470 T10678 T10677 punct -,F204V
R2471 T10679 T10670 prep with,colocalized
R2472 T10516 T10510 punct ", ",appeared
R2473 T10680 T10679 pobj calnexin,with
R2474 T10681 T10667 punct .,found
R2475 T10517 T10510 prep in,appeared
R2476 T10683 T10684 det The,%
R2477 T10684 T10687 nsubj %,appeared
R2478 T10518 T10519 det a,pattern
R2479 T10685 T10684 amod remaining,%
R2480 T10686 T10684 nummod 20,%
R2481 T10519 T10517 pobj pattern,in
R2482 T10688 T10687 prep at,appeared
R2483 T10689 T10690 det the,periphery
R2484 T10520 T10521 npadvmod punctate,perinuclear
R2485 T10690 T10688 pobj periphery,at
R2486 T10691 T10690 prep of,periphery
R2487 T10692 T10693 det the,ER
R2488 T10521 T10519 amod perinuclear,pattern
R2489 T10693 T10691 pobj ER,of
R2490 T10694 T10687 punct ", ",appeared
R2491 T10695 T10687 advcl representing,appeared
R2492 T10522 T10521 cc but,perinuclear
R2493 T10696 T10697 compound AQP2,F204V
R2494 T10697 T10695 dobj F204V,representing
R2495 T10698 T10697 punct -,F204V
R2496 T10523 T10524 punct (,column
R2497 T10699 T10700 dep that,progressed
R2498 T10700 T10697 relcl progressed,F204V
R2499 T10524 T10510 parataxis column,appeared
R2500 T10701 T10700 aux had,progressed
R2501 T10702 T10700 advmod potentially,progressed
R2502 T10703 T10700 prep beyond,progressed
R2503 T10525 T10524 dep Figure,column
R2504 T10704 T10705 det the,ER
R2505 T10705 T10703 pobj ER,beyond
R2506 T10706 T10687 punct .,appeared
R2507 T10526 T10525 nummod 4B,Figure
R2508 T10708 T10709 det This,escape
R2509 T10709 T10712 nsubj escape,was
R2510 T10527 T10524 punct ", ",column
R2511 T10710 T10709 punct “,escape
R2512 T10711 T10709 compound ER,escape
R2513 T10528 T10524 amod third,column
R2514 T10713 T10709 punct ”,escape
R2515 T10714 T10712 acomp consistent,was
R2516 T10715 T10714 prep with,consistent
R2517 T10716 T10717 det the,proportion
R2518 T10717 T10715 pobj proportion,with
R2519 T10529 T10524 punct ),column
R2520 T10718 T10717 amod small,proportion
R2521 T10530 T10510 punct .,appeared
R2522 T10719 T10717 prep of,proportion
R2523 T10720 T10721 amod mature,F204V
R2524 T10721 T10719 pobj F204V,of
R2525 T10532 T10533 prep Upon,translocated
R2526 T10722 T10721 punct ", ",F204V
R2527 T10723 T10724 amod complex,glycosylated
R2528 T10724 T10721 amod glycosylated,F204V
R2529 T10725 T10721 punct ", ",F204V
R2530 T10534 T10532 pobj stimulation,Upon
R2531 T10726 T10721 compound AQP2,F204V
R2532 T10727 T10721 punct -,F204V
R2533 T10728 T10717 prep in,proportion
R2534 T10535 T10534 prep with,stimulation
R2535 T10729 T10730 compound mutant,kidneys
R2536 T10730 T10728 pobj kidneys,in
R2537 T10731 T10732 punct (,see
R2538 T10536 T10535 pobj forskolin,with
R2539 T10732 T10712 parataxis see,was
R2540 T10733 T10732 dobj Figure,see
R2541 T10537 T10533 punct ", ",translocated
R2542 T10734 T10733 nummod 3A,Figure
R2543 T10735 T10732 punct ),see
R2544 T10736 T10712 punct .,was
R2545 T10538 T10539 det a,activator
R2546 T10738 T10739 nsubjpass Animals,affected
R2547 T10539 T10533 nsubj activator,translocated
R2548 T10740 T10738 amod heterozygous,Animals
R2549 T10741 T10740 prep for,heterozygous
R2550 T10742 T10743 det the,mutation
R2551 T10540 T10541 npadvmod cAMP,dependent
R2552 T10743 T10741 pobj mutation,for
R2553 T10744 T10743 compound Aqp2F204V,mutation
R2554 T10745 T10739 auxpass were,affected
R2555 T10541 T10539 amod dependent,activator
R2556 T10746 T10739 neg not,affected
R2557 T10747 T10739 prep in,affected
R2558 T10748 T10749 poss their,production
R2559 T10542 T10541 punct -,dependent
R2560 T10749 T10747 pobj production,in
R2561 T10750 T10749 compound urine,production
R2562 T10751 T10749 cc or,production
R2563 T10543 T10544 compound protein,kinase
R2564 T10752 T10753 compound urine,osmolality
R2565 T10753 T10749 conj osmolality,production
R2566 T10754 T10755 punct (,see
R2567 T10755 T10739 parataxis see,affected
R2568 T10756 T10757 nmod Figure,1C
R2569 T10757 T10755 dobj 1C,see
R2570 T10544 T10539 compound kinase,activator
R2571 T10758 T10757 cc and,1C
R2572 T10759 T10757 conj 1D,1C
R2573 T10760 T10755 punct ),see
R2574 T10545 T10539 punct ", ",activator
R2575 T10761 T10739 punct .,affected
R2576 T10763 T10764 nsubjpass It,shown
R2577 T10546 T10547 amod wild,type
R2578 T10765 T10764 aux has,shown
R2579 T10766 T10764 advmod also,shown
R2580 T10547 T10549 compound type,AQP2
R2581 T10767 T10764 auxpass been,shown
R2582 T10768 T10769 mark that,interact
R2583 T10769 T10764 ccomp interact,shown
R2584 T10548 T10547 punct -,type
R2585 T10770 T10771 det a,allele
R2586 T10771 T10769 nsubj allele,interact
R2587 T10772 T10771 amod recessive,allele
R2588 T10549 T10539 appos AQP2,activator
R2589 T10773 T10771 compound NDI,allele
R2590 T10774 T10771 punct ", ",allele
R2591 T10775 T10776 compound AQP2,R187C
R2592 T10550 T10533 prep to,translocated
R2593 T10776 T10771 appos R187C,allele
R2594 T10777 T10776 punct -,R187C
R2595 T10778 T10769 punct ", ",interact
R2596 T10551 T10552 det the,surface
R2597 T10552 T10550 pobj surface,to
R2598 T10553 T10552 amod apical,surface
R2599 T10779 T10769 aux does,interact
R2600 T10554 T10552 prep of,surface
R2601 T10780 T10769 neg not,interact
R2602 T10781 T10769 prep with,interact
R2603 T10782 T10783 amod wild,type
R2604 T10555 T10556 amod polarized,cells
R2605 T10783 T10785 compound type,protein
R2606 T10784 T10783 punct -,type
R2607 T10556 T10554 pobj cells,of
R2608 T10785 T10781 pobj protein,with
R2609 T10786 T10769 prep in,interact
R2610 T10787 T10786 pobj oocytes,in
R2611 T10557 T10556 compound MDCK,cells
R2612 T10788 T10789 punct [,24
R2613 T10789 T10769 parataxis 24,interact
R2614 T10790 T10789 punct ],24
R2615 T10558 T10559 punct (,column
R2616 T10791 T10769 punct ", ",interact
R2617 T10792 T10769 cc nor,interact
R2618 T10793 T10794 aux does,homo-oligomerize
R2619 T10794 T10769 conj homo-oligomerize,interact
R2620 T10795 T10794 nsubj it,homo-oligomerize
R2621 T10796 T10794 prep in,homo-oligomerize
R2622 T10559 T10533 parataxis column,translocated
R2623 T10797 T10798 compound MDCK,cells
R2624 T10798 T10796 pobj cells,in
R2625 T10799 T10800 punct [,22
R2626 T10560 T10559 dep Figure,column
R2627 T10800 T10764 parataxis 22,shown
R2628 T10801 T10800 punct ],22
R2629 T10802 T10764 punct .,shown
R2630 T10561 T10560 nummod 4B,Figure
R2631 T10804 T10805 advmod Therefore,examined
R2632 T10562 T10559 punct ", ",column
R2633 T10563 T10559 amod second,column
R2634 T10806 T10805 punct ", ",examined
R2635 T10564 T10559 punct ),column
R2636 T10807 T10805 nsubjpass kidneys,examined
R2637 T10808 T10807 prep from,kidneys
R2638 T10565 T10533 punct .,translocated
R2639 T10809 T10810 amod heterozygous,animals
R2640 T10810 T10808 pobj animals,from
R2641 T10811 T10805 auxpass were,examined
R2642 T10885 T10884 prep by,undetectable
R2643 T10812 T10805 prep for,examined
R2644 T10813 T10812 pobj evidence,for
R2645 T10814 T10813 prep of,evidence
R2646 T10815 T10816 nummod two,populations
R2647 T10886 T10885 pobj immunocytochemistry,by
R2648 T10816 T10814 pobj populations,of
R2649 T10817 T10816 prep of,populations
R2650 T10818 T10819 compound AQP2,protein
R2651 T10887 T10868 punct .,reflect
R2652 T10819 T10817 pobj protein,of
R2653 T10820 T10805 punct .,examined
R2654 T10889 T10890 advmod Alternatively,alter
R2655 T10822 T10823 advmod Surprisingly,revealed
R2656 T10824 T10823 punct ", ",revealed
R2657 T10891 T10890 punct ", ",alter
R2658 T10825 T10826 amod immunohistochemical,staining
R2659 T10826 T10823 nsubj staining,revealed
R2660 T10827 T10826 prep of,staining
R2661 T10828 T10829 nmod kidney,ducts
R2662 T10892 T10893 det the,presence
R2663 T10829 T10827 pobj ducts,of
R2664 T10830 T10829 amod collecting,ducts
R2665 T10831 T10829 prep from,ducts
R2666 T10893 T10890 nsubj presence,alter
R2667 T10832 T10833 nmod Aqp2F204V,mice
R2668 T10833 T10831 pobj mice,from
R2669 T10834 T10832 punct /,Aqp2F204V
R2670 T10835 T10832 punct +,Aqp2F204V
R2671 T10836 T10837 det a,pattern
R2672 T10837 T10823 dobj pattern,revealed
R2673 T10894 T10893 prep of,presence
R2674 T10838 T10839 advmod remarkably,similar
R2675 T10839 T10837 amod similar,pattern
R2676 T10840 T10839 prep to,similar
R2677 T10895 T10896 amod wild,type
R2678 T10841 T10842 amod wild,type
R2679 T10842 T10840 pobj type,to
R2680 T10843 T10844 punct (,Figure
R2681 T10844 T10823 parataxis Figure,revealed
R2682 T10845 T10844 nummod 5A,Figure
R2683 T10846 T10844 punct ),Figure
R2684 T10847 T10823 punct .,revealed
R2685 T10896 T10898 compound type,protein
R2686 T10849 T10850 nsubj AQP2,translocated
R2687 T10897 T10896 punct -,type
R2688 T10851 T10850 advmod completely,translocated
R2689 T10852 T10850 prep to,translocated
R2690 T10898 T10894 pobj protein,of
R2691 T10853 T10854 det the,surface
R2692 T10854 T10852 pobj surface,to
R2693 T10855 T10854 amod apical,surface
R2694 T10856 T10854 compound cell,surface
R2695 T10899 T10890 aux may,alter
R2696 T10857 T10850 prep upon,translocated
R2697 T10858 T10859 compound dDAVP,stimulation
R2698 T10859 T10857 pobj stimulation,upon
R2699 T10900 T10901 det the,localization
R2700 T10860 T10850 punct .,translocated
R2701 T10901 T10890 dobj localization,alter
R2702 T10862 T10863 det This,pattern
R2703 T10863 T10868 nsubj pattern,reflect
R2704 T10864 T10865 amod wild,type
R2705 T10902 T10901 prep of,localization
R2706 T10865 T10863 compound type,pattern
R2707 T10866 T10865 punct -,type
R2708 T10867 T10863 compound staining,pattern
R2709 T10903 T10904 det the,protein
R2710 T10869 T10868 aux may,reflect
R2711 T10904 T10902 pobj protein,of
R2712 T10870 T10868 advmod simply,reflect
R2713 T10871 T10872 det the,fact
R2714 T10872 T10868 dobj fact,reflect
R2715 T10873 T10874 mark that,makes
R2716 T10905 T10904 compound mutant,protein
R2717 T10874 T10872 acl makes,fact
R2718 T10875 T10874 csubj decreasing,makes
R2719 T10876 T10877 det the,amount
R2720 T10906 T10890 punct .,alter
R2721 T10877 T10875 dobj amount,decreasing
R2722 T10878 T10877 prep of,amount
R2723 T10879 T10880 compound mutant,protein
R2724 T10880 T10878 pobj protein,of
R2725 T10881 T10875 prep by,decreasing
R2726 T10882 T10881 pobj half,by
R2727 T10908 T10909 advmod Indeed,proposed
R2728 T10883 T10884 nsubj it,undetectable
R2729 T10884 T10874 ccomp undetectable,makes
R2730 T10910 T10909 punct ", ",proposed
R2731 T10911 T10909 nsubj Hendriks,proposed
R2732 T10912 T10913 advmod et,al.
R2733 T10913 T10911 advmod al.,Hendriks
R2734 T10991 T10980 nsubj type,rescue
R2735 T10914 T10915 det a,mechanism
R2736 T10992 T10991 amod wild,type
R2737 T10915 T10909 dobj mechanism,proposed
R2738 T10993 T10980 aux may,rescue
R2739 T10994 T10995 det the,protein
R2740 T10916 T10915 punct “,mechanism
R2741 T10995 T10980 dobj protein,rescue
R2742 T10996 T10995 compound mutant,protein
R2743 T10997 T10998 mark as,suggested
R2744 T10917 T10918 nmod piggy,back
R2745 T10998 T10980 advcl suggested,rescue
R2746 T10999 T10998 agent by,suggested
R2747 T10918 T10915 nmod back,mechanism
R2748 T11000 T11001 det the,distribution
R2749 T11001 T10999 pobj distribution,by
R2750 T11002 T11001 amod subcellular,distribution
R2751 T11003 T11001 prep of,distribution
R2752 T10919 T10918 punct -,back
R2753 T11004 T11005 compound AQP2,protein
R2754 T11005 T11003 pobj protein,of
R2755 T10920 T10915 punct ”,mechanism
R2756 T11006 T10980 punct .,rescue
R2757 T11008 T11009 aux To,test
R2758 T10921 T10922 aux to,explain
R2759 T11009 T11010 advcl test,looked
R2760 T10922 T10909 advcl explain,proposed
R2761 T11011 T11012 det this,idea
R2762 T11012 T11009 dobj idea,test
R2763 T11013 T11010 punct ", ",looked
R2764 T11014 T11010 nsubj we,looked
R2765 T10923 T10924 det the,transport
R2766 T11015 T11010 advmod first,looked
R2767 T11016 T11010 prep for,looked
R2768 T10924 T10922 dobj transport,explain
R2769 T11017 T11018 det an,interaction
R2770 T11018 T11016 pobj interaction,for
R2771 T11019 T11018 prep between,interaction
R2772 T10925 T10924 prep of,transport
R2773 T11020 T11021 nmod mutant,proteins
R2774 T11021 T11019 pobj proteins,between
R2775 T11022 T11020 cc and,mutant
R2776 T11023 T11024 amod wild,type
R2777 T11024 T11020 conj type,mutant
R2778 T10926 T10927 amod nonglycosylated,subunits
R2779 T11025 T11024 punct -,type
R2780 T11026 T11010 prep in,looked
R2781 T11027 T11028 amod transfected,cells
R2782 T10927 T10925 pobj subunits,of
R2783 T11028 T11026 pobj cells,in
R2784 T11029 T11028 compound MDCK,cells
R2785 T11030 T11031 punct (,Figure
R2786 T10928 T10927 prep of,subunits
R2787 T11031 T11010 parataxis Figure,looked
R2788 T11032 T11031 nummod 5B,Figure
R2789 T11033 T11031 punct ),Figure
R2790 T11034 T11010 punct .,looked
R2791 T10929 T10928 pobj AQP2,of
R2792 T11036 T11037 compound MDCK,cells
R2793 T10930 T10924 prep to,transport
R2794 T11037 T11038 nsubjpass cells,transfected
R2795 T11039 T11040 advmod stably,expressing
R2796 T10931 T10932 det the,surface
R2797 T11040 T11037 acl expressing,cells
R2798 T11041 T11042 amod wild,type
R2799 T10932 T10930 pobj surface,to
R2800 T11042 T11044 compound type,AQP2
R2801 T11043 T11042 punct -,type
R2802 T11044 T11040 dobj AQP2,expressing
R2803 T10933 T10932 compound cell,surface
R2804 T11045 T11038 auxpass were,transfected
R2805 T11046 T11038 advmod transiently,transfected
R2806 T11047 T11038 prep with,transfected
R2807 T10934 T10924 prep by,transport
R2808 T11048 T11049 compound GFP,constructs
R2809 T11049 T11047 pobj constructs,with
R2810 T11050 T11049 compound expression,constructs
R2811 T10935 T10936 amod glycosylated,subunits
R2812 T11051 T11049 acl encoding,constructs
R2813 T11052 T11053 npadvmod GFP,tagged
R2814 T10936 T10934 pobj subunits,by
R2815 T11053 T11055 amod tagged,AQP2
R2816 T11054 T11053 punct -,tagged
R2817 T11055 T11051 dobj AQP2,encoding
R2818 T10937 T10938 punct [,22
R2819 T11056 T11057 amod wild,type
R2820 T11057 T11055 compound type,AQP2
R2821 T11058 T11057 punct -,type
R2822 T10938 T10922 parataxis 22,explain
R2823 T11059 T11055 punct ", ",AQP2
R2824 T11060 T11061 compound AQP2,F204V
R2825 T11061 T11055 conj F204V,AQP2
R2826 T11062 T11061 punct -,F204V
R2827 T11063 T11061 punct ", ",F204V
R2828 T11064 T11061 cc or,F204V
R2829 T10939 T10938 punct ],22
R2830 T11065 T11061 conj GFP,F204V
R2831 T11066 T11065 advmod alone,GFP
R2832 T10940 T10909 punct .,proposed
R2833 T10942 T10943 nsubjpass It,shown
R2834 T11067 T11038 punct .,transfected
R2835 T10944 T10943 aux has,shown
R2836 T11069 T11070 nsubj Antibodies,coimmunoprecipitated
R2837 T11071 T11069 prep against,Antibodies
R2838 T11072 T11071 pobj GFP,against
R2839 T10945 T10943 advmod also,shown
R2840 T11073 T11074 amod wild,type
R2841 T11074 T11076 compound type,AQP2
R2842 T10946 T10943 auxpass been,shown
R2843 T11075 T11074 punct -,type
R2844 T11076 T11070 dobj AQP2,coimmunoprecipitated
R2845 T11077 T11078 advmod when,transfected
R2846 T10947 T10948 mark that,rescue
R2847 T11078 T11070 advcl transfected,coimmunoprecipitated
R2848 T11079 T11080 compound AQP2,GFP
R2849 T10948 T10943 ccomp rescue,shown
R2850 T11080 T11078 nsubjpass GFP,transfected
R2851 T11081 T11080 punct -,GFP
R2852 T11082 T11080 cc or,GFP
R2853 T10949 T10950 amod wild,type
R2854 T11083 T11084 compound AQP2,GFP
R2855 T11084 T11080 conj GFP,GFP
R2856 T11085 T11084 punct -,GFP
R2857 T10950 T10952 compound type,protein
R2858 T11086 T11084 compound F204V,GFP
R2859 T11087 T11084 punct -,GFP
R2860 T10951 T10950 punct -,type
R2861 T11088 T11078 auxpass was,transfected
R2862 T11089 T11078 advmod transiently,transfected
R2863 T11090 T11078 punct ", ",transfected
R2864 T10952 T10948 nsubj protein,rescue
R2865 T11091 T11078 cc but,transfected
R2866 T11092 T11091 neg not,but
R2867 T11093 T11094 advmod when,transfected
R2868 T10953 T10952 compound AQP2,protein
R2869 T11094 T11078 conj transfected,transfected
R2870 T11095 T11094 nsubjpass GFP,transfected
R2871 T10954 T10948 aux can,rescue
R2872 T11096 T11095 prep by,GFP
R2873 T10955 T10956 det a,protein
R2874 T10956 T10948 dobj protein,rescue
R2875 T10957 T10958 npadvmod translocation,defective
R2876 T11097 T11096 pobj itself,by
R2877 T11098 T11094 auxpass was,transfected
R2878 T11099 T11094 advmod transiently,transfected
R2879 T11100 T11094 prep into,transfected
R2880 T11101 T11102 compound MDCK,cells
R2881 T10958 T10956 amod defective,protein
R2882 T11102 T11100 pobj cells,into
R2883 T11103 T11104 advmod stably,expressing
R2884 T11104 T11102 acl expressing,cells
R2885 T10959 T10958 punct -,defective
R2886 T11105 T11106 amod wild,type
R2887 T11106 T11108 compound type,AQP2
R2888 T11107 T11106 punct -,type
R2889 T10960 T10956 compound mutant,protein
R2890 T11108 T11104 dobj AQP2,expressing
R2891 T11109 T11110 punct (,blots
R2892 T10961 T10956 punct ", ",protein
R2893 T11110 T11070 parataxis blots,coimmunoprecipitated
R2894 T11111 T11110 dep Figure,blots
R2895 T11112 T11111 nummod 5B,Figure
R2896 T10962 T10963 compound AQP2,P262L
R2897 T11113 T11110 punct ", ",blots
R2898 T11114 T11110 amod upper,blots
R2899 T11115 T11110 punct ),blots
R2900 T10963 T10956 appos P262L,protein
R2901 T11116 T11070 punct .,coimmunoprecipitated
R2902 T10964 T10963 punct -,P262L
R2903 T11118 T11119 compound Western,blot
R2904 T11119 T11120 nsubj blot,showed
R2905 T10965 T10948 punct ", ",rescue
R2906 T11121 T11119 prep of,blot
R2907 T11122 T11123 amod total,membranes
R2908 T11123 T11121 pobj membranes,of
R2909 T10966 T10967 advmod when,coexpressed
R2910 T11124 T11125 mark that,expressed
R2911 T11125 T11120 ccomp expressed,showed
R2912 T11126 T11127 amod wild,type
R2913 T10967 T10948 advcl coexpressed,rescue
R2914 T11127 T11129 compound type,AQP2
R2915 T11128 T11127 punct -,type
R2916 T11129 T11125 nsubjpass AQP2,expressed
R2917 T10968 T10969 det the,two
R2918 T11130 T11125 auxpass is,expressed
R2919 T11131 T11125 advmod equivalently,expressed
R2920 T10969 T10967 nsubjpass two,coexpressed
R2921 T11132 T11125 prep in,expressed
R2922 T11133 T11134 det all,cases
R2923 T11134 T11132 pobj cases,in
R2924 T11135 T11134 nummod three,cases
R2925 T10970 T10967 auxpass are,coexpressed
R2926 T11136 T11137 punct (,blots
R2927 T11137 T11120 parataxis blots,showed
R2928 T11138 T11137 dep Figure,blots
R2929 T11139 T11138 nummod 5B,Figure
R2930 T11140 T11137 punct ", ",blots
R2931 T11141 T11137 amod lower,blots
R2932 T10971 T10967 prep in,coexpressed
R2933 T11142 T11137 punct ),blots
R2934 T11143 T11120 punct .,showed
R2935 T11145 T11146 mark If,interacting
R2936 T10972 T10973 compound MDCK,cells
R2937 T10973 T10971 pobj cells,in
R2938 T11146 T11155 advcl interacting,is
R2939 T10974 T10975 punct [,25
R2940 T11147 T11148 amod wild,type
R2941 T11148 T11150 nmod type,proteins
R2942 T11149 T11148 punct -,type
R2943 T11150 T11146 nsubj proteins,interacting
R2944 T10975 T10943 parataxis 25,shown
R2945 T11151 T11148 cc and,type
R2946 T11152 T11148 conj mutant,type
R2947 T11153 T11146 aux are,interacting
R2948 T10976 T10975 punct ],25
R2949 T11154 T11146 advmod indeed,interacting
R2950 T11156 T11146 prep in,interacting
R2951 T10977 T10943 punct .,shown
R2952 T11157 T11158 det the,cell
R2953 T11158 T11156 pobj cell,in
R2954 T11159 T11155 punct ", ",is
R2955 T10979 T10980 prep In,rescue
R2956 T11160 T11161 det this,interaction
R2957 T11161 T11155 nsubj interaction,is
R2958 T11162 T11155 acomp sufficient,is
R2959 T11163 T11164 aux to,rescue
R2960 T10981 T10982 det the,ducts
R2961 T11164 T11162 xcomp rescue,sufficient
R2962 T11165 T11166 det the,localization
R2963 T11166 T11164 dobj localization,rescue
R2964 T10982 T10979 pobj ducts,In
R2965 T11167 T11166 prep of,localization
R2966 T11168 T11169 compound mutant,protein
R2967 T11169 T11167 pobj protein,of
R2968 T11170 T11155 punct ?,is
R2969 T10983 T10982 amod collecting,ducts
R2970 T11172 T11173 aux To,answer
R2971 T11173 T11174 advcl answer,used
R2972 T10984 T10982 prep from,ducts
R2973 T11175 T11176 det this,question
R2974 T11176 T11173 dobj question,answer
R2975 T10985 T10986 nmod Aqp2F204V,mice
R2976 T11177 T11174 punct ", ",used
R2977 T11178 T11174 nsubj we,used
R2978 T10986 T10984 pobj mice,from
R2979 T11179 T11180 compound MDCK,cells
R2980 T11180 T11174 dobj cells,used
R2981 T11181 T11182 advmod stably,transfected
R2982 T11182 T11180 acl transfected,cells
R2983 T11183 T11182 prep with,transfected
R2984 T11184 T11185 amod wild,type
R2985 T10987 T10985 punct /,Aqp2F204V
R2986 T11185 T11187 compound type,AQP2
R2987 T11186 T11185 punct -,type
R2988 T11187 T11183 pobj AQP2,with
R2989 T10988 T10985 punct +,Aqp2F204V
R2990 T11188 T11187 acl expressing,AQP2
R2991 T11189 T11188 dobj vector,expressing
R2992 T11190 T11188 cc or,expressing
R2993 T10989 T10980 punct ", ",rescue
R2994 T11191 T11188 conj with,expressing
R2995 T11192 T11193 amod empty,vector
R2996 T11193 T11191 pobj vector,with
R2997 T10990 T10991 det the,type
R2998 T11194 T11174 punct .,used
R2999 T11196 T11197 prep On,transfected
R3000 T11203 T11197 advmod transiently,transfected
R3001 T11198 T11196 pobj top,On
R3002 T11199 T11198 prep of,top
R3003 T11200 T11199 pobj these,of
R3004 T11201 T11197 punct ", ",transfected
R3005 T11204 T11205 nmod AQP2,GFP
R3006 T11202 T11197 nsubj we,transfected
R3007 T11205 T11207 nmod GFP,constructs
R3008 T11206 T11205 punct -,GFP
R3009 T11207 T11197 dobj constructs,transfected
R3010 T11208 T11205 cc or,GFP
R3011 T11308 T11306 compound F204V,GFP
R3012 T11309 T11306 punct -,GFP
R3013 T11209 T11210 compound AQP2,GFP
R3014 T11310 T11295 prep to,localized
R3015 T11311 T11312 det the,surface
R3016 T11312 T11310 pobj surface,to
R3017 T11210 T11205 conj GFP,GFP
R3018 T11313 T11312 amod apical,surface
R3019 T11314 T11295 prep to,localized
R3020 T11211 T11210 punct -,GFP
R3021 T11315 T11316 amod varying,degrees
R3022 T11316 T11314 pobj degrees,to
R3023 T11317 T11318 punct (,images
R3024 T11212 T11210 compound F204V,GFP
R3025 T11318 T11295 parataxis images,localized
R3026 T11319 T11318 dep Figure,images
R3027 T11320 T11319 nummod 5C,Figure
R3028 T11213 T11210 punct -,GFP
R3029 T11321 T11318 punct ", ",images
R3030 T11322 T11318 amod lower,images
R3031 T11214 T11207 compound expression,constructs
R3032 T11323 T11318 amod right,images
R3033 T11324 T11325 punct [,i
R3034 T11325 T11318 parataxis i,images
R3035 T11215 T11197 punct .,transfected
R3036 T11326 T11327 punct –,iii
R3037 T11327 T11325 prep iii,i
R3038 T11217 T11218 compound AQP2,GFP
R3039 T11328 T11325 punct ],i
R3040 T11329 T11318 punct ),images
R3041 T11330 T11295 punct .,localized
R3042 T11218 T11220 nsubj GFP,localized
R3043 T11332 T11333 det The,images
R3044 T11333 T11336 nsubj images,shows
R3045 T11219 T11218 punct -,GFP
R3046 T11334 T11335 amod lower,right
R3047 T11335 T11333 amod right,images
R3048 T11221 T11220 prep to,localized
R3049 T11337 T11333 prep of,images
R3050 T11338 T11337 pobj Figure,of
R3051 T11339 T11338 nummod 5C,Figure
R3052 T11222 T11223 det the,surface
R3053 T11340 T11341 nummod three,cells
R3054 T11341 T11336 dobj cells,shows
R3055 T11342 T11341 prep from,cells
R3056 T11223 T11221 pobj surface,to
R3057 T11343 T11344 det a,transfection
R3058 T11344 T11342 pobj transfection,from
R3059 T11345 T11344 amod single,transfection
R3060 T11224 T11223 amod apical,surface
R3061 T11346 T11336 punct .,shows
R3062 T11348 T11349 det The,first
R3063 T11225 T11220 prep upon,localized
R3064 T11349 T11350 nsubj first,is
R3065 T11226 T11227 compound forskolin,stimulation
R3066 T11351 T11352 det a,cell
R3067 T11352 T11350 attr cell,is
R3068 T11353 T11352 amod nontransfected,cell
R3069 T11227 T11225 pobj stimulation,upon
R3070 T11354 T11355 dep that,shows
R3071 T11355 T11352 relcl shows,cell
R3072 T11356 T11357 det the,localization
R3073 T11228 T11229 mark whether,transfected
R3074 T11357 T11355 dobj localization,shows
R3075 T11358 T11357 prep of,localization
R3076 T11359 T11360 det the,AQP2
R3077 T11229 T11220 advcl transfected,localized
R3078 T11360 T11358 pobj AQP2,of
R3079 T11230 T11229 nsubjpass it,transfected
R3080 T11361 T11362 advmod stably,expressing
R3081 T11231 T11229 auxpass was,transfected
R3082 T11362 T11360 amod expressing,AQP2
R3083 T11363 T11364 amod wild,type
R3084 T11232 T11229 advmod transiently,transfected
R3085 T11364 T11360 compound type,AQP2
R3086 T11365 T11364 punct -,type
R3087 T11366 T11360 punct ", ",AQP2
R3088 T11367 T11368 dep which,is
R3089 T11368 T11360 relcl is,AQP2
R3090 T11233 T11229 prep into,transfected
R3091 T11369 T11368 acomp apical,is
R3092 T11370 T11368 prep upon,is
R3093 T11371 T11372 compound forskolin,stimulation
R3094 T11234 T11235 npadvmod vector,only
R3095 T11372 T11370 pobj stimulation,upon
R3096 T11373 T11350 punct .,is
R3097 T11235 T11237 amod only,cells
R3098 T11375 T11376 det The,two
R3099 T11376 T11378 nsubj two,show
R3100 T11377 T11376 amod next,two
R3101 T11236 T11235 punct -,only
R3102 T11379 T11378 dobj expression,show
R3103 T11380 T11379 prep of,expression
R3104 T11237 T11233 pobj cells,into
R3105 T11381 T11382 preconj both,AQP2
R3106 T11382 T11380 pobj AQP2,of
R3107 T11238 T11239 punct (,images
R3108 T11383 T11382 det the,AQP2
R3109 T11384 T11382 amod stable,AQP2
R3110 T11385 T11386 amod wild,type
R3111 T11239 T11237 parataxis images,cells
R3112 T11386 T11382 compound type,AQP2
R3113 T11387 T11386 punct -,type
R3114 T11388 T11382 cc and,AQP2
R3115 T11240 T11239 dep Figure,images
R3116 T11389 T11390 det the,GFP
R3117 T11390 T11382 conj GFP,AQP2
R3118 T11391 T11390 amod transient,GFP
R3119 T11241 T11240 nummod 5C,Figure
R3120 T11392 T11390 compound AQP2,GFP
R3121 T11393 T11390 punct -,GFP
R3122 T11394 T11390 compound F204V,GFP
R3123 T11242 T11239 punct ", ",images
R3124 T11395 T11390 punct -,GFP
R3125 T11396 T11378 punct .,show
R3126 T11243 T11239 amod upper,images
R3127 T11398 T11399 prep In,was
R3128 T11399 T11411 ccomp was,were
R3129 T11244 T11239 amod left,images
R3130 T11400 T11398 pobj cell,In
R3131 T11401 T11400 punct (,cell
R3132 T11402 T11400 nummod ii,cell
R3133 T11403 T11400 punct ),cell
R3134 T11245 T11239 punct ),images
R3135 T11404 T11399 punct ", ",was
R3136 T11405 T11399 nsubj localization,was
R3137 T11406 T11405 prep of,localization
R3138 T11407 T11408 amod wild,type
R3139 T11408 T11410 compound type,AQP2
R3140 T11409 T11408 punct -,type
R3141 T11246 T11233 cc or,into
R3142 T11410 T11406 pobj AQP2,of
R3143 T11412 T11399 acomp indistinguishable,was
R3144 T11247 T11233 conj into,into
R3145 T11248 T11249 amod wild,type
R3146 T11249 T11251 compound type,cells
R3147 T11413 T11412 prep from,indistinguishable
R3148 T11414 T11415 compound AQP2,GFP
R3149 T11250 T11249 punct -,type
R3150 T11251 T11247 pobj cells,into
R3151 T11415 T11413 pobj GFP,from
R3152 T11416 T11415 punct -,GFP
R3153 T11252 T11251 compound AQP2,cells
R3154 T11417 T11415 compound F204V,GFP
R3155 T11418 T11415 punct -,GFP
R3156 T11419 T11411 punct ;,were
R3157 T11253 T11254 punct (,Figure
R3158 T11420 T11411 nsubj both,were
R3159 T11421 T11411 acomp apical,were
R3160 T11254 T11251 parataxis Figure,cells
R3161 T11422 T11411 prep upon,were
R3162 T11423 T11424 compound forskolin,stimulation
R3163 T11255 T11254 nummod 5C,Figure
R3164 T11424 T11422 pobj stimulation,upon
R3165 T11425 T11411 punct .,were
R3166 T11256 T11254 punct ", ",Figure
R3167 T11427 T11428 mark Although,was
R3168 T11428 T11431 advcl was,localized
R3169 T11257 T11258 amod upper,right
R3170 T11429 T11430 det the,effect
R3171 T11430 T11428 nsubj effect,was
R3172 T11258 T11254 amod right,Figure
R3173 T11432 T11428 acomp subtle,was
R3174 T11433 T11428 prep in,was
R3175 T11259 T11254 punct ),Figure
R3176 T11434 T11433 pobj cell,in
R3177 T11435 T11434 punct (,cell
R3178 T11436 T11434 nummod iii,cell
R3179 T11260 T11220 punct .,localized
R3180 T11437 T11434 punct ),cell
R3181 T11438 T11431 punct ", ",localized
R3182 T11439 T11440 compound AQP2,GFP
R3183 T11262 T11263 compound AQP2,GFP
R3184 T11440 T11431 nsubjpass GFP,localized
R3185 T11441 T11440 punct -,GFP
R3186 T11442 T11440 compound F204V,GFP
R3187 T11443 T11440 punct -,GFP
R3188 T11444 T11431 auxpass was,localized
R3189 T11445 T11431 advmod partly,localized
R3190 T11446 T11431 prep to,localized
R3191 T11263 T11267 nsubj GFP,showed
R3192 T11447 T11448 det the,surface
R3193 T11448 T11446 pobj surface,to
R3194 T11449 T11448 amod apical,surface
R3195 T11264 T11263 punct -,GFP
R3196 T11450 T11431 punct .,localized
R3197 T11452 T11453 advmod Generally,was
R3198 T11265 T11263 compound F204V,GFP
R3199 T11454 T11453 punct ", ",was
R3200 T11266 T11263 punct -,GFP
R3201 T11455 T11456 det the,localization
R3202 T11456 T11453 nsubj localization,was
R3203 T11457 T11456 prep of,localization
R3204 T11268 T11267 punct ", ",showed
R3205 T11458 T11459 compound AQP2,GFP
R3206 T11459 T11457 pobj GFP,of
R3207 T11460 T11459 punct -,GFP
R3208 T11461 T11459 compound F204V,GFP
R3209 T11269 T11270 advmod when,expressed
R3210 T11462 T11459 punct -,GFP
R3211 T11463 T11453 advmod clearly,was
R3212 T11464 T11465 advmod more,apical
R3213 T11270 T11267 advcl expressed,showed
R3214 T11465 T11453 acomp apical,was
R3215 T11466 T11467 advmod when,expressed
R3216 T11467 T11453 advcl expressed,was
R3217 T11271 T11270 prep by,expressed
R3218 T11468 T11469 amod wild,type
R3219 T11469 T11471 compound type,AQP2
R3220 T11470 T11469 punct -,type
R3221 T11272 T11273 amod transient,transfection
R3222 T11471 T11467 nsubjpass AQP2,expressed
R3223 T11472 T11467 auxpass was,expressed
R3224 T11473 T11467 advmod also,expressed
R3225 T11273 T11271 pobj transfection,by
R3226 T11474 T11453 punct .,was
R3227 T11274 T11270 prep into,expressed
R3228 T11476 T11477 aux To,confirm
R3229 T11477 T11478 advcl confirm,transfected
R3230 T11275 T11276 npadvmod vector,only
R3231 T11479 T11480 det these,results
R3232 T11480 T11477 dobj results,confirm
R3233 T11276 T11277 amod only,cells
R3234 T11481 T11477 advmod biochemically,confirm
R3235 T11482 T11478 punct ", ",transfected
R3236 T11483 T11478 nsubj we,transfected
R3237 T11277 T11274 pobj cells,into
R3238 T11484 T11485 det the,lines
R3239 T11485 T11478 dobj lines,transfected
R3240 T11486 T11485 amod same,lines
R3241 T11487 T11485 compound cell,lines
R3242 T11488 T11485 punct (,lines
R3243 T11278 T11267 punct ", ",showed
R3244 T11489 T11490 amod wild,type
R3245 T11490 T11492 compound type,AQP2
R3246 T11491 T11490 punct -,type
R3247 T11279 T11280 det a,pattern
R3248 T11492 T11485 appos AQP2,lines
R3249 T11493 T11492 cc or,AQP2
R3250 T11494 T11492 conj vector,AQP2
R3251 T11280 T11267 dobj pattern,showed
R3252 T11495 T11485 punct ),lines
R3253 T11496 T11478 prep with,transfected
R3254 T11497 T11498 compound F204V,GFP
R3255 T11281 T11280 amod diffuse,pattern
R3256 T11498 T11496 pobj GFP,with
R3257 T11499 T11498 punct -,GFP
R3258 T11500 T11478 punct ", ",transfected
R3259 T11282 T11280 amod cytoplasmic,pattern
R3260 T11501 T11478 conj biotinylated,transfected
R3261 T11283 T11280 compound distribution,pattern
R3262 T11502 T11503 compound surface,proteins
R3263 T11284 T11285 punct (,left
R3264 T11503 T11501 dobj proteins,biotinylated
R3265 T11504 T11501 prep after,biotinylated
R3266 T11505 T11506 compound forskolin,stimulation
R3267 T11285 T11267 parataxis left,showed
R3268 T11506 T11504 pobj stimulation,after
R3269 T11507 T11501 punct ", ",biotinylated
R3270 T11286 T11285 dep Figure,left
R3271 T11508 T11501 cc and,biotinylated
R3272 T11509 T11501 conj precipitated,biotinylated
R3273 T11510 T11511 det the,proteins
R3274 T11287 T11286 nummod 5C,Figure
R3275 T11511 T11509 dobj proteins,precipitated
R3276 T11512 T11511 amod biotinylated,proteins
R3277 T11288 T11285 punct ", ",left
R3278 T11513 T11514 punct (,Figure
R3279 T11514 T11509 parataxis Figure,precipitated
R3280 T11515 T11514 nummod 5D,Figure
R3281 T11289 T11285 amod lower,left
R3282 T11516 T11514 punct ),Figure
R3283 T11517 T11478 punct .,transfected
R3284 T11290 T11285 punct ),left
R3285 T11291 T11267 punct .,showed
R3286 T11293 T11294 advmod When,expressed
R3287 T11519 T11520 compound AQP2,GFP
R3288 T11520 T11524 nsubjpass GFP,expressed
R3289 T11294 T11295 advcl expressed,localized
R3290 T11521 T11520 punct -,GFP
R3291 T11522 T11520 compound F204V,GFP
R3292 T11523 T11520 punct -,GFP
R3293 T11525 T11524 auxpass is,expressed
R3294 T11296 T11294 prep in,expressed
R3295 T11526 T11527 advmod approximately,equally
R3296 T11527 T11524 advmod equally,expressed
R3297 T11528 T11524 prep in,expressed
R3298 T11529 T11530 det both,lines
R3299 T11530 T11528 pobj lines,in
R3300 T11297 T11298 amod wild,type
R3301 T11531 T11530 compound cell,lines
R3302 T11532 T11533 punct (,cells
R3303 T11533 T11524 parataxis cells,expressed
R3304 T11298 T11300 compound type,cells
R3305 T11534 T11533 dep Figure,cells
R3306 T11535 T11534 nummod 5D,Figure
R3307 T11536 T11533 punct ", ",cells
R3308 T11299 T11298 punct -,type
R3309 T11537 T11533 amod total,cells
R3310 T11538 T11533 punct ),cells
R3311 T11300 T11296 pobj cells,in
R3312 T11539 T11524 punct ", ",expressed
R3313 T11540 T11524 cc but,expressed
R3314 T11541 T11542 auxpass is,biotinylated
R3315 T11301 T11300 compound AQP2,cells
R3316 T11542 T11524 conj biotinylated,expressed
R3317 T11543 T11544 advmod only,expressed
R3318 T11544 T11542 ccomp expressed,biotinylated
R3319 T11302 T11295 punct ", ",localized
R3320 T11545 T11544 advmod when,expressed
R3321 T11546 T11547 amod wild,type
R3322 T11547 T11549 compound type,AQP2
R3323 T11303 T11295 advmod however,localized
R3324 T11548 T11547 punct -,type
R3325 T11549 T11544 nsubjpass AQP2,expressed
R3326 T11550 T11544 auxpass is,expressed
R3327 T11304 T11295 punct ", ",localized
R3328 T11551 T11544 advmod also,expressed
R3329 T11552 T11553 punct (,Figure
R3330 T11553 T11542 parataxis Figure,biotinylated
R3331 T11305 T11306 compound AQP2,GFP
R3332 T11554 T11553 nummod 5D,Figure
R3333 T11555 T11553 punct ", ",Figure
R3334 T11556 T11557 npadvmod surface,biotinylated
R3335 T11306 T11295 nsubj GFP,localized
R3336 T11557 T11553 amod biotinylated,Figure
R3337 T11558 T11553 punct ),Figure
R3338 T11559 T11524 punct .,expressed
R3339 T11307 T11306 punct -,GFP
R3340 T11561 T11562 mark Since,are
R3341 T11562 T11567 advcl are,indicate
R3342 T11563 T11564 advmod only,proteins
R3343 T11564 T11562 nsubj proteins,are
R3344 T11565 T11566 compound cell,surface
R3345 T11566 T11564 compound surface,proteins
R3346 T11568 T11562 acomp accessible,are
R3347 T11569 T11568 prep to,accessible
R3348 T11570 T11569 pobj biotin,to
R3349 T11571 T11567 punct ", ",indicate
R3350 T11572 T11573 det these,results
R3351 T11573 T11567 nsubj results,indicate
R3352 T11574 T11575 mark that,transported
R3353 T11575 T11567 ccomp transported,indicate
R3354 T11576 T11577 compound AQP2,F204V
R3355 T11577 T11575 nsubjpass F204V,transported
R3356 T11578 T11577 punct -,F204V
R3357 T11579 T11575 auxpass is,transported
R3358 T11580 T11575 prep to,transported
R3359 T11581 T11582 det the,surface
R3360 T11582 T11580 pobj surface,to
R3361 T11583 T11582 compound cell,surface
R3362 T11584 T11585 advmod when,is
R3363 T11585 T11575 advcl is,transported
R3364 T11586 T11587 amod wild,type
R3365 T11587 T11589 compound type,AQP2
R3366 T11588 T11587 punct -,type
R3367 T11589 T11585 nsubj AQP2,is
R3368 T11590 T11585 acomp present,is
R3369 T11591 T11585 punct ", ",is
R3370 T11592 T11585 cc but,is
R3371 T11593 T11592 neg not,but
R3372 T11594 T11585 conj on,is
R3373 T11595 T11596 poss its,own
R3374 T11596 T11594 pobj own,on
R3375 T11597 T11567 punct .,indicate
R3378 T14172 T14173 compound Aqp2F204,F204V
R3379 T14173 T14175 compound F204V,mice
R3380 T14174 T14173 punct /,F204V
R3381 T14175 T14176 nsubj mice,are
R3382 T14177 T14176 acomp viable,are
R3383 T14178 T14176 cc and,are
R3384 T14179 T14176 conj grow,are
R3385 T14180 T14179 cc and,grow
R3386 T14181 T14179 conj reproduce,grow
R3387 T14182 T14181 advmod normally,reproduce
R3388 T14183 T14176 punct .,are
R3389 T14185 T14186 nsubj They,are
R3390 T14187 T14186 punct ", ",are
R3391 T14188 T14186 advmod however,are
R3392 T14189 T14186 punct ", ",are
R3393 T14190 T14191 advmod severely,defective
R3394 T14191 T14186 acomp defective,are
R3395 T14192 T14191 prep in,defective
R3396 T14193 T14194 poss their,ability
R3397 T14194 T14192 pobj ability,in
R3398 T14195 T14196 aux to,concentrate
R3399 T14196 T14194 acl concentrate,ability
R3400 T14197 T14196 dobj urine,concentrate
R3401 T14198 T14186 punct ", ",are
R3402 T14199 T14186 advcl leading,are
R3403 T14200 T14199 prep to,leading
R3404 T14201 T14202 amod increased,output
R3405 T14202 T14200 pobj output,to
R3406 T14203 T14202 compound urine,output
R3407 T14204 T14202 cc and,output
R3408 T14205 T14206 compound water,intake
R3409 T14206 T14202 conj intake,output
R3410 T14207 T14186 punct ", ",are
R3411 T14208 T14209 advmod thus,making
R3412 T14209 T14186 advcl making,are
R3413 T14210 T14211 nsubj them,model
R3414 T14211 T14209 ccomp model,making
R3415 T14212 T14211 det the,model
R3416 T14213 T14211 amod first,model
R3417 T14214 T14211 compound mouse,model
R3418 T14215 T14211 prep of,model
R3419 T14216 T14215 pobj NDI,of
R3420 T14217 T14218 aux to,survive
R3421 T14218 T14211 advcl survive,model
R3422 T14219 T14218 prep to,survive
R3423 T14220 T14219 pobj maturity,to
R3424 T14221 T14186 punct .,are
R3425 T14223 T14224 prep In,caused
R3426 T14225 T14223 pobj humans,In
R3427 T14226 T14224 punct ", ",caused
R3428 T14227 T14224 nsubjpass NDI,caused
R3429 T14228 T14224 auxpass is,caused
R3430 T14229 T14224 agent by,caused
R3431 T14230 T14229 pobj mutations,by
R3432 T14231 T14230 prep in,mutations
R3433 T14232 T14231 pobj Avpr2,in
R3434 T14233 T14232 cc or,Avpr2
R3435 T14234 T14232 conj Aqp2,Avpr2
R3436 T14235 T14224 punct .,caused
R3437 T14237 T14238 nsubj Knockout,gave
R3438 T14239 T14237 prep of,Knockout
R3439 T14240 T14241 det the,gene
R3440 T14241 T14239 pobj gene,of
R3441 T14242 T14243 npadvmod X,linked
R3442 T14243 T14241 amod linked,gene
R3443 T14244 T14243 punct -,linked
R3444 T14245 T14241 compound Avpr2,gene
R3445 T14246 T14237 prep in,Knockout
R3446 T14247 T14246 pobj mice,in
R3447 T14248 T14249 punct [,15
R3448 T14249 T14237 parataxis 15,Knockout
R3449 T14250 T14249 punct ],15
R3450 T14251 T14252 det an,phenotype
R3451 T14252 T14238 dobj phenotype,gave
R3452 T14253 T14254 npadvmod NDI,like
R3453 T14254 T14252 amod like,phenotype
R3454 T14255 T14254 punct -,like
R3455 T14256 T14238 prep in,gave
R3456 T14257 T14258 amod male,neonates
R3457 T14258 T14256 pobj neonates,in
R3458 T14259 T14258 punct ", ",neonates
R3459 T14260 T14258 amod hemizygous,neonates
R3460 T14261 T14238 punct ", ",gave
R3461 T14262 T14238 cc but,gave
R3462 T14263 T14264 det the,phenotype
R3463 T14264 T14265 nsubjpass phenotype,assessed
R3464 T14265 T14238 conj assessed,gave
R3465 T14266 T14265 aux could,assessed
R3466 T14267 T14265 neg not,assessed
R3467 T14268 T14265 auxpass be,assessed
R3468 T14269 T14265 prep in,assessed
R3469 T14270 T14269 pobj adults,in
R3470 T14271 T14272 mark as,died
R3471 T14272 T14265 advcl died,assessed
R3472 T14273 T14274 det the,mice
R3473 T14274 T14272 nsubj mice,died
R3474 T14275 T14272 prep within,died
R3475 T14276 T14277 nummod 1,wk
R3476 T14277 T14275 pobj wk,within
R3477 T14278 T14277 prep of,wk
R3478 T14279 T14278 pobj birth,of
R3479 T14280 T14265 punct .,assessed
R3480 T14282 T14283 det The,females
R3481 T14283 T14286 nsubj females,showed
R3482 T14284 T14283 amod adult,females
R3483 T14285 T14283 amod heterozygous,females
R3484 T14287 T14288 det a,tendency
R3485 T14288 T14286 dobj tendency,showed
R3486 T14289 T14288 amod mild,tendency
R3487 T14290 T14288 prep toward,tendency
R3488 T14291 T14292 amod increased,output
R3489 T14292 T14290 pobj output,toward
R3490 T14293 T14292 amod urinary,output
R3491 T14294 T14292 cc and,output
R3492 T14295 T14296 compound water,intake
R3493 T14296 T14292 conj intake,output
R3494 T14297 T14292 cc and,output
R3495 T14298 T14299 amod decreased,osmolality
R3496 T14299 T14292 conj osmolality,output
R3497 T14300 T14299 compound urine,osmolality
R3498 T14301 T14286 punct .,showed
R3499 T14303 T14304 nsubjpass Knockout,reported
R3500 T14305 T14303 prep of,Knockout
R3501 T14306 T14307 det the,gene
R3502 T14307 T14305 pobj gene,of
R3503 T14308 T14307 compound mouse,gene
R3504 T14309 T14307 compound Aqp2,gene
R3505 T14310 T14304 aux has,reported
R3506 T14311 T14304 neg not,reported
R3507 T14312 T14304 auxpass been,reported
R3508 T14313 T14304 punct .,reported
R3509 T14315 T14316 det A,in
R3510 T14316 T14319 nsubjpass in,made
R3511 T14317 T14316 compound knock,in
R3512 T14318 T14316 punct -,in
R3513 T14320 T14316 prep of,in
R3514 T14321 T14322 det a,mutation
R3515 T14322 T14320 pobj mutation,of
R3516 T14323 T14322 amod human,mutation
R3517 T14324 T14325 npadvmod disease,causing
R3518 T14325 T14322 amod causing,mutation
R3519 T14326 T14325 punct -,causing
R3520 T14327 T14322 punct (,mutation
R3521 T14328 T14322 appos T126M,mutation
R3522 T14329 T14322 punct ),mutation
R3523 T14330 T14319 punct ", ",made
R3524 T14331 T14319 advmod however,made
R3525 T14332 T14319 punct ", ",made
R3526 T14333 T14319 aux has,made
R3527 T14334 T14319 auxpass been,made
R3528 T14335 T14336 punct [,18
R3529 T14336 T14319 parataxis 18,made
R3530 T14337 T14336 punct ],18
R3531 T14338 T14319 punct .,made
R3532 T14340 T14341 det These,mice
R3533 T14341 T14342 nsubj mice,have
R3534 T14343 T14344 det a,defect
R3535 T14344 T14342 dobj defect,have
R3536 T14345 T14344 amod severe,defect
R3537 T14346 T14347 npadvmod urine,concentrating
R3538 T14347 T14344 amod concentrating,defect
R3539 T14348 T14347 punct -,concentrating
R3540 T14349 T14344 acl resulting,defect
R3541 T14350 T14349 prep in,resulting
R3542 T14351 T14350 pobj dehydration,in
R3543 T14352 T14351 cc and,dehydration
R3544 T14353 T14351 conj death,dehydration
R3545 T14354 T14349 prep within,resulting
R3546 T14355 T14356 nummod 1,wk
R3547 T14356 T14354 pobj wk,within
R3548 T14357 T14356 prep of,wk
R3549 T14358 T14357 pobj birth,of
R3550 T14359 T14342 punct .,have
R3551 T14361 T14362 advmod Curiously,localize
R3552 T14363 T14362 punct ", ",localize
R3553 T14364 T14365 compound AQP2,T126M
R3554 T14365 T14362 nsubj T126M,localize
R3555 T14366 T14365 punct -,T126M
R3556 T14367 T14362 aux does,localize
R3557 T14368 T14362 advmod properly,localize
R3558 T14369 T14362 prep in,localize
R3559 T14370 T14371 advmod at,least
R3560 T14371 T14372 advmod least,subset
R3561 T14372 T14369 pobj subset,in
R3562 T14373 T14372 det a,subset
R3563 T14374 T14372 prep of,subset
R3564 T14375 T14374 pobj cells,of
R3565 T14376 T14362 punct .,localize
R3566 T14378 T14379 det The,morphology
R3567 T14379 T14384 nsubj morphology,makes
R3568 T14380 T14381 advmod grossly,abnormal
R3569 T14381 T14379 amod abnormal,morphology
R3570 T14382 T14383 amod collecting,duct
R3571 T14383 T14379 compound duct,morphology
R3572 T14385 T14386 nsubj it,impossible
R3573 T14386 T14384 ccomp impossible,makes
R3574 T14387 T14388 aux to,pinpoint
R3575 T14388 T14386 advcl pinpoint,impossible
R3576 T14389 T14390 det the,defect
R3577 T14390 T14388 dobj defect,pinpoint
R3578 T14391 T14390 amod molecular,defect
R3579 T14392 T14388 prep in,pinpoint
R3580 T14393 T14394 det these,mice
R3581 T14394 T14392 pobj mice,in
R3582 T14395 T14394 amod knock,mice
R3583 T14396 T14395 punct -,knock
R3584 T14397 T14395 prt in,knock
R3585 T14398 T14384 punct .,makes
R3586 T14400 T14401 det The,in
R3587 T14401 T14405 nsubj in,shows
R3588 T14402 T14401 compound T126M,in
R3589 T14403 T14401 compound knock,in
R3590 T14404 T14401 punct -,in
R3591 T14406 T14405 advmod clearly,shows
R3592 T14407 T14408 mark that,is
R3593 T14408 T14405 ccomp is,shows
R3594 T14409 T14408 nsubj Aqp2,is
R3595 T14410 T14411 det an,gene
R3596 T14411 T14408 attr gene,is
R3597 T14412 T14411 amod essential,gene
R3598 T14413 T14414 punct [,18
R3599 T14414 T14405 parataxis 18,shows
R3600 T14415 T14414 punct ],18
R3601 T14416 T14405 punct .,shows
R3602 T14418 T14419 det The,fact
R3603 T14419 T14420 nsubj fact,shows
R3604 T14421 T14422 mark that,survive
R3605 T14422 T14419 acl survive,fact
R3606 T14423 T14424 poss our,mice
R3607 T14424 T14422 nsubj mice,survive
R3608 T14425 T14426 preconj either,possesses
R3609 T14426 T14420 advcl possesses,shows
R3610 T14427 T14426 mark that,possesses
R3611 T14428 T14429 compound AQP2,F204V
R3612 T14429 T14426 nsubj F204V,possesses
R3613 T14430 T14429 punct -,F204V
R3614 T14431 T14432 det some,ability
R3615 T14432 T14426 dobj ability,possesses
R3616 T14433 T14432 amod residual,ability
R3617 T14434 T14435 npadvmod water,transporting
R3618 T14435 T14432 amod transporting,ability
R3619 T14436 T14426 cc or,possesses
R3620 T14437 T14438 mark that,are
R3621 T14438 T14426 conj are,possesses
R3622 T14439 T14438 expl there,are
R3623 T14440 T14441 npadvmod AVP,independent
R3624 T14441 T14443 amod independent,pathways
R3625 T14442 T14441 punct -,independent
R3626 T14443 T14438 attr pathways,are
R3627 T14444 T14443 prep for,pathways
R3628 T14445 T14446 compound water,reabsorption
R3629 T14446 T14444 pobj reabsorption,for
R3630 T14447 T14420 punct .,shows
R3631 T14449 T14450 amod Residual,activity
R3632 T14450 T14451 nsubj activity,is
R3633 T14452 T14450 prep of,activity
R3634 T14453 T14454 compound AQP2,F204V
R3635 T14454 T14452 pobj F204V,of
R3636 T14455 T14454 punct -,F204V
R3637 T14456 T14451 acomp likely,is
R3638 T14457 T14451 punct ", ",is
R3639 T14458 T14459 mark as,show
R3640 T14459 T14451 advcl show,is
R3641 T14460 T14461 compound mutant,animals
R3642 T14461 T14459 nsubj animals,show
R3643 T14462 T14463 det some,response
R3644 T14463 T14459 dobj response,show
R3645 T14464 T14463 amod small,response
R3646 T14465 T14463 prep to,response
R3647 T14466 T14465 pobj dDAVP,to
R3648 T14467 T14459 punct ", ",show
R3649 T14468 T14469 mark although,remains
R3650 T14469 T14459 advcl remains,show
R3651 T14470 T14471 npadvmod dDAVP,stimulated
R3652 T14471 T14473 amod stimulated,osmolality
R3653 T14472 T14471 punct -,stimulated
R3654 T14473 T14469 nsubj osmolality,remains
R3655 T14474 T14473 compound urine,osmolality
R3656 T14475 T14476 advmod quite,low
R3657 T14476 T14469 acomp low,remains
R3658 T14477 T14451 punct .,is
R3659 T14479 T14480 nsubj Immunostaining,shows
R3660 T14481 T14479 prep of,Immunostaining
R3661 T14482 T14481 pobj kidney,of
R3662 T14483 T14484 mark that,transport
R3663 T14484 T14480 ccomp transport,shows
R3664 T14485 T14486 compound AQP2,F204V
R3665 T14486 T14484 nsubj F204V,transport
R3666 T14487 T14486 punct -,F204V
R3667 T14488 T14484 aux does,transport
R3668 T14489 T14484 neg not,transport
R3669 T14490 T14484 advmod efficiently,transport
R3670 T14491 T14484 dobj water,transport
R3671 T14492 T14484 punct ", ",transport
R3672 T14493 T14494 mark because,fails
R3673 T14494 T14484 advcl fails,transport
R3674 T14495 T14494 nsubj it,fails
R3675 T14496 T14497 aux to,localize
R3676 T14497 T14494 xcomp localize,fails
R3677 T14498 T14497 prep to,localize
R3678 T14499 T14500 det the,surface
R3679 T14500 T14498 pobj surface,to
R3680 T14501 T14500 amod apical,surface
R3681 T14502 T14500 compound cell,surface
R3682 T14503 T14497 prep after,localize
R3683 T14504 T14505 compound dDAVP,treatment
R3684 T14505 T14503 pobj treatment,after
R3685 T14506 T14480 punct .,shows
R3686 T14508 T14509 det Some,activity
R3687 T14509 T14511 nsubj activity,imply
R3688 T14510 T14509 amod residual,activity
R3689 T14512 T14509 prep of,activity
R3690 T14513 T14512 pobj AQP2,of
R3691 T14514 T14511 aux would,imply
R3692 T14515 T14516 mark that,getting
R3693 T14516 T14511 ccomp getting,imply
R3694 T14517 T14518 det some,portion
R3695 T14518 T14516 nsubj portion,getting
R3696 T14519 T14518 amod small,portion
R3697 T14520 T14518 punct ", ",portion
R3698 T14521 T14518 amod undetectable,portion
R3699 T14522 T14518 prep of,portion
R3700 T14523 T14524 det the,protein
R3701 T14524 T14522 pobj protein,of
R3702 T14525 T14524 compound mutant,protein
R3703 T14526 T14516 aux is,getting
R3704 T14527 T14516 prep to,getting
R3705 T14528 T14529 det the,surface
R3706 T14529 T14527 pobj surface,to
R3707 T14530 T14529 compound cell,surface
R3708 T14531 T14511 punct .,imply
R3709 T14533 T14534 det The,experiment
R3710 T14534 T14537 nsubj experiment,suggests
R3711 T14535 T14534 compound surface,experiment
R3712 T14536 T14534 compound biotinylation,experiment
R3713 T14538 T14539 punct (,Figure
R3714 T14539 T14534 parataxis Figure,experiment
R3715 T14540 T14539 nummod 5D,Figure
R3716 T14541 T14539 punct ),Figure
R3717 T14542 T14543 mark that,gets
R3718 T14543 T14537 ccomp gets,suggests
R3719 T14544 T14545 det no,protein
R3720 T14545 T14543 nsubj protein,gets
R3721 T14546 T14545 compound mutant,protein
R3722 T14547 T14543 prep to,gets
R3723 T14548 T14549 det the,surface
R3724 T14549 T14547 pobj surface,to
R3725 T14550 T14537 punct ", ",suggests
R3726 T14551 T14537 cc but,suggests
R3727 T14552 T14553 nsubj this,reflect
R3728 T14553 T14537 conj reflect,suggests
R3729 T14554 T14553 aux does,reflect
R3730 T14555 T14553 neg not,reflect
R3731 T14556 T14553 advmod necessarily,reflect
R3732 T14557 T14558 det the,situation
R3733 T14558 T14553 dobj situation,reflect
R3734 T14559 T14560 advmod in,vivo
R3735 T14560 T14558 advmod vivo,situation
R3736 T14561 T14553 punct .,reflect
R3737 T14563 T14564 mark While,be
R3738 T14564 T14572 advcl be,shows
R3739 T14565 T14566 det this,fraction
R3740 T14566 T14564 nsubj fraction,be
R3741 T14567 T14566 amod small,fraction
R3742 T14568 T14566 prep of,fraction
R3743 T14569 T14568 pobj protein,of
R3744 T14570 T14564 aux may,be
R3745 T14571 T14564 neg not,be
R3746 T14573 T14564 acomp detectable,be
R3747 T14574 T14564 prep by,be
R3748 T14575 T14574 pobj immunofluorescence,by
R3749 T14576 T14572 punct ", ",shows
R3750 T14577 T14578 compound Western,blotting
R3751 T14578 T14572 nsubj blotting,shows
R3752 T14579 T14580 mark that,progress
R3753 T14580 T14572 ccomp progress,shows
R3754 T14581 T14582 det some,protein
R3755 T14582 T14580 nsubj protein,progress
R3756 T14583 T14582 compound mutant,protein
R3757 T14584 T14580 aux does,progress
R3758 T14585 T14580 prep beyond,progress
R3759 T14586 T14587 det the,ER
R3760 T14587 T14585 pobj ER,beyond
R3761 T14588 T14589 punct (,species
R3762 T14589 T14572 parataxis species,shows
R3763 T14590 T14591 quantmod 35,45
R3764 T14591 T14593 nummod 45,kDa
R3765 T14592 T14591 punct –,45
R3766 T14593 T14589 compound kDa,species
R3767 T14594 T14589 prep in,species
R3768 T14595 T14594 pobj Figure,in
R3769 T14596 T14595 nummod 3A,Figure
R3770 T14597 T14589 punct ),species
R3771 T14598 T14572 punct .,shows
R3772 T14600 T14601 prep Compared,is
R3773 T14602 T14600 prep to,Compared
R3774 T14603 T14604 amod wild,type
R3775 T14604 T14602 pobj type,to
R3776 T14605 T14604 punct -,type
R3777 T14606 T14601 punct ", ",is
R3778 T14607 T14608 compound mutant,protein
R3779 T14608 T14601 nsubj protein,is
R3780 T14609 T14601 acomp enriched,is
R3781 T14610 T14609 prep in,enriched
R3782 T14611 T14612 det the,form
R3783 T14612 T14610 pobj form,in
R3784 T14613 T14614 amod high,mannose
R3785 T14614 T14612 nmod mannose,form
R3786 T14615 T14614 punct -,mannose
R3787 T14616 T14612 punct ", ",form
R3788 T14617 T14618 npadvmod core,glycosylated
R3789 T14618 T14612 amod glycosylated,form
R3790 T14619 T14618 punct -,glycosylated
R3791 T14620 T14621 punct (,kDa
R3792 T14621 T14612 parataxis kDa,form
R3793 T14622 T14621 nummod 31,kDa
R3794 T14623 T14621 punct ),kDa
R3795 T14624 T14609 cc and,enriched
R3796 T14625 T14609 conj deficient,enriched
R3797 T14626 T14625 prep in,deficient
R3798 T14627 T14628 amod nonglycosylated,forms
R3799 T14628 T14626 pobj forms,in
R3800 T14629 T14630 punct (,kDa
R3801 T14630 T14627 parataxis kDa,nonglycosylated
R3802 T14631 T14630 nummod 29,kDa
R3803 T14632 T14630 punct ),kDa
R3804 T14633 T14627 cc and,nonglycosylated
R3805 T14634 T14635 amod complex,glycosylated
R3806 T14635 T14627 conj glycosylated,nonglycosylated
R3807 T14636 T14637 punct (,kDa
R3808 T14637 T14635 parataxis kDa,glycosylated
R3809 T14638 T14639 quantmod 35,45
R3810 T14639 T14637 nummod 45,kDa
R3811 T14640 T14639 punct –,45
R3812 T14641 T14637 punct ),kDa
R3813 T14642 T14601 punct .,is
R3814 T14644 T14645 det The,presence
R3815 T14645 T14646 nsubj presence,indicates
R3816 T14647 T14645 prep of,presence
R3817 T14648 T14649 det a,amount
R3818 T14649 T14647 pobj amount,of
R3819 T14650 T14649 amod reduced,amount
R3820 T14651 T14650 cc but,reduced
R3821 T14652 T14650 conj detectable,reduced
R3822 T14653 T14649 prep of,amount
R3823 T14654 T14653 pobj protein,of
R3824 T14655 T14654 prep in,protein
R3825 T14656 T14657 det the,range
R3826 T14657 T14655 pobj range,in
R3827 T14658 T14659 quantmod 35,45
R3828 T14659 T14661 nummod 45,kDa
R3829 T14660 T14659 punct –,45
R3830 T14661 T14657 compound kDa,range
R3831 T14662 T14663 mark that,transported
R3832 T14663 T14646 ccomp transported,indicates
R3833 T14664 T14665 compound mutant,protein
R3834 T14665 T14663 nsubjpass protein,transported
R3835 T14666 T14663 auxpass is,transported
R3836 T14667 T14663 prep out,transported
R3837 T14668 T14667 prep of,out
R3838 T14669 T14670 det the,ER
R3839 T14670 T14668 pobj ER,of
R3840 T14671 T14663 punct ", ",transported
R3841 T14672 T14673 cc but,with
R3842 T14673 T14663 prep with,transported
R3843 T14674 T14675 advmod greatly,reduced
R3844 T14675 T14676 amod reduced,efficiency
R3845 T14676 T14673 pobj efficiency,with
R3846 T14677 T14646 punct .,indicates
R3847 T14679 T14680 nsubj Colocalization,shows
R3848 T14681 T14679 prep of,Colocalization
R3849 T14682 T14683 compound AQP2,F204V
R3850 T14683 T14681 pobj F204V,of
R3851 T14684 T14683 punct -,F204V
R3852 T14685 T14679 prep with,Colocalization
R3853 T14686 T14687 det the,protein
R3854 T14687 T14685 pobj protein,with
R3855 T14688 T14687 compound ER,protein
R3856 T14689 T14687 appos calnexin,protein
R3857 T14690 T14679 prep in,Colocalization
R3858 T14691 T14692 amod transfected,cells
R3859 T14692 T14690 pobj cells,in
R3860 T14693 T14692 compound MDCK,cells
R3861 T14694 T14695 mark that,progress
R3862 T14695 T14680 ccomp progress,shows
R3863 T14696 T14695 punct ", ",progress
R3864 T14697 T14698 mark while,trapped
R3865 T14698 T14695 advcl trapped,progress
R3866 T14738 T14737 prep of,fraction
R3867 T14699 T14698 nsubjpass most,trapped
R3868 T14739 T14740 compound mutant,protein
R3869 T14700 T14699 prep of,most
R3870 T14740 T14738 pobj protein,of
R3871 T14741 T14734 prep beyond,transport
R3872 T14701 T14702 det the,protein
R3873 T14742 T14743 det the,ER
R3874 T14743 T14741 pobj ER,beyond
R3875 T14744 T14734 prep in,transport
R3876 T14702 T14700 pobj protein,of
R3877 T14745 T14746 compound MDCK,cells
R3878 T14746 T14744 pobj cells,in
R3879 T14747 T14718 advmod all,are
R3880 T14703 T14702 compound mutant,protein
R3881 T14748 T14718 acomp consistent,are
R3882 T14749 T14748 prep with,consistent
R3883 T14750 T14751 det the,notion
R3884 T14704 T14698 auxpass is,trapped
R3885 T14751 T14749 pobj notion,with
R3886 T14752 T14753 mark that,is
R3887 T14753 T14751 advcl is,notion
R3888 T14705 T14698 prep in,trapped
R3889 T14754 T14755 compound AQP2,F204V
R3890 T14755 T14757 compound F204V,misfolding
R3891 T14756 T14755 punct -,F204V
R3892 T14706 T14707 det the,ER
R3893 T14757 T14753 nsubj misfolding,is
R3894 T14758 T14753 acomp limited,is
R3895 T14759 T14753 cc and,is
R3896 T14760 T14761 mark that,retain
R3897 T14761 T14753 conj retain,is
R3898 T14762 T14761 nsubj it,retain
R3899 T14707 T14705 pobj ER,in
R3900 T14763 T14761 aux may,retain
R3901 T14764 T14765 det some,activity
R3902 T14765 T14761 dobj activity,retain
R3903 T14708 T14695 punct ", ",progress
R3904 T14766 T14765 amod residual,activity
R3905 T14767 T14768 npadvmod water,transporting
R3906 T14709 T14695 nsubj some,progress
R3907 T14768 T14765 amod transporting,activity
R3908 T14769 T14718 punct .,are
R3909 T14771 T14772 advmod Evidently,is
R3910 T14710 T14695 aux does,progress
R3911 T14773 T14774 det this,activity
R3912 T14774 T14772 nsubj activity,is
R3913 T14711 T14695 prep beyond,progress
R3914 T14775 T14774 amod residual,activity
R3915 T14712 T14713 det the,ER
R3916 T14776 T14772 acomp sufficient,is
R3917 T14777 T14776 prep for,sufficient
R3918 T14713 T14711 pobj ER,beyond
R3919 T14778 T14779 det the,viability
R3920 T14779 T14777 pobj viability,for
R3921 T14780 T14779 cc and,viability
R3922 T14714 T14680 punct .,shows
R3923 T14781 T14779 conj growth,viability
R3924 T14782 T14779 prep of,viability
R3925 T14783 T14784 compound mutant,animals
R3926 T14716 T14717 amod Diminished,response
R3927 T14784 T14782 pobj animals,of
R3928 T14785 T14772 punct .,is
R3929 T14787 T14788 amod Reduced,efficiency
R3930 T14717 T14718 nsubj response,are
R3931 T14788 T14789 nsubj efficiency,explain
R3932 T14790 T14788 prep in,efficiency
R3933 T14719 T14717 prep to,response
R3934 T14791 T14790 pcomp exiting,in
R3935 T14792 T14793 det the,ER
R3936 T14793 T14791 dobj ER,exiting
R3937 T14794 T14789 aux may,explain
R3938 T14720 T14719 pobj dDAVP,to
R3939 T14795 T14796 advmod why,enriched
R3940 T14796 T14789 ccomp enriched,explain
R3941 T14797 T14798 compound AQP2,F204V
R3942 T14721 T14717 punct ", ",response
R3943 T14798 T14796 nsubjpass F204V,enriched
R3944 T14799 T14798 punct -,F204V
R3945 T14800 T14796 auxpass is,enriched
R3946 T14801 T14796 prep in,enriched
R3947 T14802 T14803 det the,form
R3948 T14803 T14801 pobj form,in
R3949 T14722 T14723 amod diminished,abundance
R3950 T14804 T14805 nummod 31,kDa
R3951 T14805 T14803 nmod kDa,form
R3952 T14806 T14807 amod high,mannose
R3953 T14807 T14803 nmod mannose,form
R3954 T14723 T14717 conj abundance,response
R3955 T14808 T14807 punct -,mannose
R3956 T14809 T14803 amod glycosylated,form
R3957 T14810 T14789 punct .,explain
R3958 T14724 T14723 prep of,abundance
R3959 T14725 T14726 amod mature,protein
R3960 T14812 T14813 det The,oligosaccharide
R3961 T14813 T14818 nsubjpass oligosaccharide,added
R3962 T14814 T14815 amod high,mannose
R3963 T14815 T14813 compound mannose,oligosaccharide
R3964 T14816 T14815 punct -,mannose
R3965 T14726 T14724 pobj protein,of
R3966 T14817 T14813 compound core,oligosaccharide
R3967 T14727 T14726 amod glycosylated,protein
R3968 T14819 T14818 auxpass is,added
R3969 T14820 T14818 prep in,added
R3970 T14821 T14822 det the,ER
R3971 T14728 T14723 prep in,abundance
R3972 T14822 T14820 pobj ER,in
R3973 T14823 T14818 cc and,added
R3974 T14824 T14825 auxpass is,modified
R3975 T14729 T14730 compound mutant,animals
R3976 T14825 T14818 conj modified,added
R3977 T14826 T14825 advmod later,modified
R3978 T14730 T14728 pobj animals,in
R3979 T14827 T14825 cc and,modified
R3980 T14828 T14825 conj elaborated,modified
R3981 T14829 T14828 prep in,elaborated
R3982 T14731 T14723 punct ", ",abundance
R3983 T14830 T14831 det the,apparatus
R3984 T14831 T14829 pobj apparatus,in
R3985 T14732 T14723 cc and,abundance
R3986 T14832 T14831 compound Golgi,apparatus
R3987 T14833 T14834 punct [,26
R3988 T14834 T14825 parataxis 26,modified
R3989 T14733 T14734 det the,transport
R3990 T14835 T14834 punct ],26
R3991 T14836 T14818 punct .,added
R3992 T14734 T14723 conj transport,abundance
R3993 T14838 T14839 det The,increase
R3994 T14839 T14840 nsubj increase,reflect
R3995 T14735 T14734 prep of,transport
R3996 T14841 T14839 prep in,increase
R3997 T14842 T14843 det the,form
R3998 T14843 T14841 pobj form,in
R3999 T14736 T14737 det a,fraction
R4000 T14737 T14735 pobj fraction,of
R4001 T14950 T14949 aux can,explain
R4002 T14844 T14845 amod high,mannose
R4003 T14845 T14843 nmod mannose,form
R4004 T14846 T14845 punct -,mannose
R4005 T14847 T14843 amod glycosylated,form
R4006 T14951 T14949 neg not,explain
R4007 T14848 T14843 prep of,form
R4008 T14849 T14850 compound AQP2,F204V
R4009 T14850 T14848 pobj F204V,of
R4010 T14952 T14953 det the,lack
R4011 T14851 T14850 punct -,F204V
R4012 T14852 T14840 aux may,reflect
R4013 T14853 T14840 advmod simply,reflect
R4014 T14953 T14949 dobj lack,explain
R4015 T14854 T14855 poss its,presence
R4016 T14954 T14953 prep of,lack
R4017 T14855 T14840 dobj presence,reflect
R4018 T14856 T14855 amod prolonged,presence
R4019 T14857 T14855 prep in,presence
R4020 T14858 T14859 det the,ER
R4021 T14955 T14956 det any,protein
R4022 T14859 T14857 pobj ER,in
R4023 T14860 T14855 cc and,presence
R4024 T14861 T14855 conj exposure,presence
R4025 T14956 T14954 pobj protein,of
R4026 T14957 T14958 npadvmod ER,retained
R4027 T14862 T14861 prep to,exposure
R4028 T14863 T14864 compound oligosaccharyl,transferase
R4029 T14864 T14862 pobj transferase,to
R4030 T14958 T14956 amod retained,protein
R4031 T14865 T14840 punct .,reflect
R4032 T14867 T14868 mark While,explains
R4033 T14959 T14958 punct -,retained
R4034 T14868 T14873 advcl explains,is
R4035 T14960 T14956 compound mutant,protein
R4036 T14869 T14870 amod improper,localization
R4037 T14870 T14868 nsubj localization,explains
R4038 T14871 T14870 prep of,localization
R4039 T14961 T14949 punct .,explain
R4040 T14872 T14871 pobj AQP2,of
R4041 T14963 T14964 advmod Rather,suggests
R4042 T14874 T14875 det the,phenotype
R4043 T14875 T14868 dobj phenotype,explains
R4044 T14876 T14875 prep of,phenotype
R4045 T14877 T14878 amod homozygous,mice
R4046 T14878 T14876 pobj mice,of
R4047 T14965 T14964 punct ", ",suggests
R4048 T14879 T14878 compound mutant,mice
R4049 T14880 T14873 punct ", ",is
R4050 T14881 T14882 det the,lack
R4051 T14882 T14873 nsubj lack,is
R4052 T14883 T14882 amod complete,lack
R4053 T14884 T14882 prep of,lack
R4054 T14885 T14886 det a,phenotype
R4055 T14886 T14884 pobj phenotype,of
R4056 T14966 T14967 det the,phenotype
R4057 T14887 T14882 prep in,lack
R4058 T14888 T14889 amod heterozygous,mice
R4059 T14889 T14887 pobj mice,in
R4060 T14967 T14964 nsubj phenotype,suggests
R4061 T14890 T14891 advmod more,difficult
R4062 T14891 T14873 acomp difficult,is
R4063 T14892 T14893 aux to,explain
R4064 T14968 T14967 prep of,phenotype
R4065 T14893 T14891 advcl explain,difficult
R4066 T14894 T14873 punct .,is
R4067 T14969 T14970 det the,animals
R4068 T14896 T14897 advmod Physiologically,have
R4069 T14970 T14968 pobj animals,of
R4070 T14898 T14897 punct ", ",have
R4071 T14899 T14900 amod heterozygous,mice
R4072 T14900 T14897 nsubj mice,have
R4073 T14971 T14970 nmod Aqp2F204V,animals
R4074 T14901 T14902 det no,symptoms
R4075 T14902 T14897 dobj symptoms,have
R4076 T14903 T14904 punct (,see
R4077 T14972 T14971 punct /,Aqp2F204V
R4078 T14904 T14897 parataxis see,have
R4079 T14905 T14904 dobj Figure,see
R4080 T14973 T14971 punct +,Aqp2F204V
R4081 T14906 T14905 nummod 1C,Figure
R4082 T14907 T14904 punct ),see
R4083 T14908 T14897 punct ", ",have
R4084 T14909 T14897 cc and,have
R4085 T14974 T14975 mark that,rescued
R4086 T14910 T14911 nsubj they,are
R4087 T14911 T14897 conj are,have
R4088 T14975 T14964 ccomp rescued,suggests
R4089 T14912 T14911 acomp indistinguishable,are
R4090 T14913 T14912 prep from,indistinguishable
R4091 T14914 T14915 amod wild,type
R4092 T14976 T14977 det the,protein
R4093 T14915 T14913 pobj type,from
R4094 T14916 T14911 prep on,are
R4095 T14917 T14916 pobj immunostaining,on
R4096 T14977 T14975 nsubjpass protein,rescued
R4097 T14918 T14917 prep of,immunostaining
R4098 T14919 T14918 pobj kidneys,of
R4099 T14920 T14921 punct (,Figure
R4100 T14978 T14977 compound mutant,protein
R4101 T14921 T14911 parataxis Figure,are
R4102 T14922 T14921 nummod 5A,Figure
R4103 T14923 T14921 punct ),Figure
R4104 T14924 T14911 punct .,are
R4105 T14979 T14975 aux is,rescued
R4106 T14926 T14927 det The,presence
R4107 T14927 T14928 nsubj presence,explain
R4108 T14980 T14975 auxpass being,rescued
R4109 T14929 T14927 prep of,presence
R4110 T14930 T14931 nummod 50,%
R4111 T14931 T14929 pobj %,of
R4112 T14981 T14975 agent by,rescued
R4113 T14932 T14931 prep of,%
R4114 T14933 T14934 det the,amount
R4115 T14934 T14932 pobj amount,of
R4116 T14982 T14983 det the,protein
R4117 T14935 T14934 amod normal,amount
R4118 T14936 T14934 prep of,amount
R4119 T14937 T14938 amod wild,type
R4120 T14938 T14940 compound type,protein
R4121 T14983 T14981 pobj protein,by
R4122 T14939 T14938 punct -,type
R4123 T14940 T14936 pobj protein,of
R4124 T14984 T14985 amod wild,type
R4125 T14941 T14928 aux may,explain
R4126 T14942 T14943 det the,lack
R4127 T14943 T14928 dobj lack,explain
R4128 T14944 T14943 prep of,lack
R4129 T14985 T14983 compound type,protein
R4130 T14945 T14944 pobj symptoms,of
R4131 T14946 T14928 punct ", ",explain
R4132 T14947 T14928 cc but,explain
R4133 T14986 T14985 punct -,type
R4134 T14948 T14949 nsubj it,explain
R4135 T14987 T14964 punct .,suggests
R4136 T14949 T14928 conj explain,explain
R4137 T14989 T14990 advmod Indeed,demonstrated
R4138 T14991 T14990 punct ", ",demonstrated
R4139 T14992 T14993 compound de,Mattia
R4140 T14993 T14990 nsubj Mattia,demonstrated
R4141 T14994 T14995 advmod et,al.
R4142 T15056 T15055 pobj this,of
R4143 T15057 T15053 punct ", ",interact
R4144 T14995 T14993 advmod al.,Mattia
R4145 T15058 T15059 compound AQP2,F204V
R4146 T15059 T15053 nsubj F204V,interact
R4147 T15060 T15059 punct -,F204V
R4148 T14996 T14997 punct (,26
R4149 T15061 T15053 aux can,interact
R4150 T15062 T15053 prep with,interact
R4151 T14997 T14993 parataxis 26,Mattia
R4152 T15063 T15064 amod wild,type
R4153 T15064 T15066 compound type,AQP2
R4154 T15065 T15064 punct -,type
R4155 T15066 T15062 pobj AQP2,with
R4156 T15067 T15068 punct (,Figure
R4157 T15068 T15053 parataxis Figure,interact
R4158 T14998 T14997 punct ),26
R4159 T15069 T15068 nummod 5B,Figure
R4160 T15070 T15068 punct ),Figure
R4161 T15071 T15053 punct ", ",interact
R4162 T15072 T15053 cc and,interact
R4163 T14999 T14990 aux have,demonstrated
R4164 T15073 T15074 advmod when,coexpressed
R4165 T15074 T15075 advcl coexpressed,reach
R4166 T15000 T15001 mark that,translocate
R4167 T15075 T15053 conj reach,interact
R4168 T15076 T15074 prep with,coexpressed
R4169 T15077 T15078 amod wild,type
R4170 T15001 T14990 advcl translocate,demonstrated
R4171 T15078 T15080 compound type,protein
R4172 T15079 T15078 punct -,type
R4173 T15080 T15076 pobj protein,with
R4174 T15002 T15003 nummod one,allele
R4175 T15081 T15075 punct ", ",reach
R4176 T15082 T15083 compound AQP2,F204V
R4177 T15083 T15075 nsubj F204V,reach
R4178 T15003 T15001 nsubj allele,translocate
R4179 T15084 T15083 punct -,F204V
R4180 T15085 T15075 aux can,reach
R4181 T15086 T15087 det the,surface
R4182 T15004 T15003 amod recessive,allele
R4183 T15087 T15075 dobj surface,reach
R4184 T15088 T15087 compound cell,surface
R4185 T15089 T15090 punct (,panel
R4186 T15005 T15003 prep of,allele
R4187 T15090 T15075 parataxis panel,reach
R4188 T15091 T15090 nmod Figure,panel
R4189 T15092 T15091 nummod 5C,Figure
R4190 T15093 T15094 npadvmod bottom,right
R4191 T15006 T15005 pobj Aqp2,of
R4192 T15094 T15090 amod right,panel
R4193 T15095 T15090 cc and,panel
R4194 T15096 T15090 conj 5D,panel
R4195 T15007 T15003 punct ", ",allele
R4196 T15097 T15090 punct ),panel
R4197 T15098 T15053 punct .,interact
R4198 T15008 T15003 appos P262L,allele
R4199 T15100 T15101 mark Although,demonstrated
R4200 T15101 T15105 advcl demonstrated,show
R4201 T15009 T15001 punct ", ",translocate
R4202 T15102 T15101 nsubjpass it,demonstrated
R4203 T15103 T15101 aux has,demonstrated
R4204 T15104 T15101 auxpass been,demonstrated
R4205 T15010 T15001 aux does,translocate
R4206 T15106 T15107 mark that,fails
R4207 T15107 T15101 ccomp fails,demonstrated
R4208 T15108 T15109 det a,allele
R4209 T15109 T15107 nsubj allele,fails
R4210 T15110 T15109 amod recessive,allele
R4211 T15011 T15001 neg not,translocate
R4212 T15111 T15109 punct (,allele
R4213 T15112 T15109 acl encoding,allele
R4214 T15113 T15114 compound AQP2,R187C
R4215 T15012 T15001 advmod properly,translocate
R4216 T15114 T15112 dobj R187C,encoding
R4217 T15115 T15114 punct -,R187C
R4218 T15116 T15109 punct ),allele
R4219 T15013 T15014 advmod when,expressed
R4220 T15117 T15109 prep of,allele
R4221 T15118 T15117 pobj NDI,of
R4222 T15014 T15001 advcl expressed,translocate
R4223 T15119 T15120 aux to,interact
R4224 T15015 T15014 prep by,expressed
R4225 T15120 T15107 xcomp interact,fails
R4226 T15121 T15120 prep with,interact
R4227 T15122 T15123 amod wild,type
R4228 T15016 T15015 pobj itself,by
R4229 T15123 T15125 compound type,AQP2
R4230 T15124 T15123 punct -,type
R4231 T15125 T15121 pobj AQP2,with
R4232 T15017 T15014 prep in,expressed
R4233 T15126 T15127 punct [,6
R4234 T15127 T15101 parataxis 6,demonstrated
R4235 T15018 T15019 compound MDCK,cells
R4236 T15128 T15127 punct ],6
R4237 T15129 T15105 punct ", ",show
R4238 T15019 T15017 pobj cells,in
R4239 T15130 T15105 advmod here,show
R4240 T15131 T15105 nsubj we,show
R4241 T15132 T15133 mark that,interact
R4242 T15133 T15105 ccomp interact,show
R4243 T15020 T15001 punct ", ",translocate
R4244 T15134 T15135 compound AQP2,F204V
R4245 T15135 T15133 nsubj F204V,interact
R4246 T15021 T15001 cc but,translocate
R4247 T15136 T15135 punct -,F204V
R4248 T15137 T15133 aux does,interact
R4249 T15138 T15133 prep with,interact
R4250 T15022 T15023 mark that,localizes
R4251 T15139 T15140 det the,protein
R4252 T15140 T15138 pobj protein,with
R4253 T15141 T15142 amod wild,type
R4254 T15023 T15001 conj localizes,translocate
R4255 T15142 T15140 compound type,protein
R4256 T15143 T15142 punct -,type
R4257 T15144 T15133 punct ", ",interact
R4258 T15145 T15146 advmod presumably,as
R4259 T15146 T15133 prep as,interact
R4260 T15147 T15146 pobj part,as
R4261 T15024 T15023 prep in,localizes
R4262 T15148 T15147 prep of,part
R4263 T15149 T15148 pobj heterotetramers,of
R4264 T15025 T15026 det the,presence
R4265 T15150 T15133 punct ", ",interact
R4266 T15151 T15133 cc and,interact
R4267 T15152 T15133 conj represents,interact
R4268 T15153 T15154 det a,allele
R4269 T15026 T15024 pobj presence,in
R4270 T15154 T15152 dobj allele,represents
R4271 T15155 T15154 amod rescuable,allele
R4272 T15156 T15152 punct ", ",represents
R4273 T15027 T15026 prep of,presence
R4274 T15157 T15158 preconj both,vitro
R4275 T15158 T15152 advmod vitro,represents
R4276 T15028 T15029 amod wild,type
R4277 T15159 T15158 advmod in,vitro
R4278 T15160 T15158 cc and,vitro
R4279 T15029 T15031 compound type,protein
R4280 T15030 T15029 punct -,type
R4281 T15031 T15027 pobj protein,of
R4282 T15161 T15162 advmod in,vivo
R4283 T15162 T15158 conj vivo,vitro
R4284 T15032 T15023 punct ", ",localizes
R4285 T15163 T15105 punct .,show
R4286 T15165 T15166 csubj Immunostaining,shows
R4287 T15033 T15023 nsubj it,localizes
R4288 T15034 T15023 advmod normally,localizes
R4289 T15167 T15168 det the,kidneys
R4290 T15168 T15165 dobj kidneys,Immunostaining
R4291 T15169 T15168 prep of,kidneys
R4292 T15170 T15171 amod homozygous,mice
R4293 T15035 T14990 punct .,demonstrated
R4294 T15171 T15169 pobj mice,of
R4295 T15172 T15173 compound Aqp2F204V,F204V
R4296 T15173 T15171 compound F204V,mice
R4297 T15037 T15038 det The,mechanism
R4298 T15174 T15173 punct /,F204V
R4299 T15175 T15176 mark that,mediate
R4300 T15176 T15166 ccomp mediate,shows
R4301 T15177 T15178 det the,cells
R4302 T15038 T15040 nsubj mechanism,seems
R4303 T15178 T15176 nsubj cells,mediate
R4304 T15179 T15180 npadvmod mutant,expressing
R4305 T15039 T15038 amod same,mechanism
R4306 T15180 T15178 amod expressing,cells
R4307 T15181 T15180 punct -,expressing
R4308 T15182 T15183 amod collecting,duct
R4309 T15183 T15178 compound duct,cells
R4310 T15184 T15176 aux can,mediate
R4311 T15185 T15176 neg not,mediate
R4312 T15186 T15187 compound water,reabsorption
R4313 T15041 T15042 aux to,apply
R4314 T15187 T15176 dobj reabsorption,mediate
R4315 T15188 T15176 punct ", ",mediate
R4316 T15189 T15190 mark because,fails
R4317 T15190 T15176 advcl fails,mediate
R4318 T15191 T15190 nsubj it,fails
R4319 T15042 T15040 xcomp apply,seems
R4320 T15192 T15193 aux to,insert
R4321 T15193 T15190 xcomp insert,fails
R4322 T15043 T15044 advmod in,vivo
R4323 T15194 T15193 prep into,insert
R4324 T15195 T15196 det the,membrane
R4325 T15196 T15194 pobj membrane,into
R4326 T15044 T15042 advmod vivo,apply
R4327 T15197 T15196 amod apical,membrane
R4328 T15198 T15196 compound plasma,membrane
R4329 T15199 T15193 prep in,insert
R4330 T15045 T15042 prep with,apply
R4331 T15200 T15199 pobj response,in
R4332 T15046 T15047 nmod Aqp2F204V,mice
R4333 T15047 T15045 pobj mice,with
R4334 T15048 T15046 punct /,Aqp2F204V
R4335 T15201 T15200 prep to,response
R4336 T15049 T15046 punct +,Aqp2F204V
R4337 T15050 T15040 punct .,seems
R4338 T15202 T15201 pobj dDAVP,to
R4339 T15203 T15166 punct .,shows
R4340 T15052 T15053 prep In,interact
R4341 T15205 T15206 nsubj This,is
R4342 T15054 T15052 pobj support,In
R4343 T15207 T15208 det this,proof
R4344 T15208 T15206 attr proof,is
R4345 T15209 T15208 amod first,proof
R4346 T15055 T15054 prep of,support
R4347 T15210 T15211 advmod in,vivo
R4348 T15211 T15208 amod vivo,proof
R4349 T15212 T15208 prep of,proof
R4350 T15267 T15268 advmod Thus,proposed
R4351 T15213 T15214 det a,hypothesis
R4352 T15214 T15212 pobj hypothesis,of
R4353 T15215 T15216 amod long,standing
R4354 T15216 T15214 amod standing,hypothesis
R4355 T15217 T15216 punct -,standing
R4356 T15269 T15268 punct ", ",proposed
R4357 T15218 T15219 dep that,comes
R4358 T15219 T15214 relcl comes,hypothesis
R4359 T15220 T15219 prep from,comes
R4360 T15221 T15222 advmod in,vitro
R4361 T15222 T15223 amod vitro,studies
R4362 T15223 T15220 pobj studies,from
R4363 T15224 T15223 prep with,studies
R4364 T15270 T15268 nsubjpass misfolding,proposed
R4365 T15225 T15226 amod recessive,mutations
R4366 T15226 T15224 pobj mutations,with
R4367 T15227 T15226 compound Aqp2,mutations
R4368 T15228 T15206 punct .,is
R4369 T15271 T15270 punct ", ",misfolding
R4370 T15230 T15231 nsubj Transfection,shows
R4371 T15272 T15270 conj retention,misfolding
R4372 T15232 T15230 prep into,Transfection
R4373 T15233 T15234 compound MDCK,cells
R4374 T15273 T15272 prep in,retention
R4375 T15234 T15232 pobj cells,into
R4376 T15235 T15230 prep of,Transfection
R4377 T15236 T15235 pobj any,of
R4378 T15274 T15275 det the,ER
R4379 T15237 T15236 prep of,any
R4380 T15238 T15239 amod several,mutations
R4381 T15239 T15237 pobj mutations,of
R4382 T15275 T15273 pobj ER,in
R4383 T15240 T15239 compound Aqp2,mutations
R4384 T15241 T15239 acl corresponding,mutations
R4385 T15276 T15272 punct ", ",retention
R4386 T15242 T15241 prep to,corresponding
R4387 T15243 T15244 amod recessive,alleles
R4388 T15244 T15242 pobj alleles,to
R4389 T15245 T15244 amod human,alleles
R4390 T15277 T15272 cc and,retention
R4391 T15246 T15247 amod abnormal,localization
R4392 T15247 T15231 dobj localization,shows
R4393 T15278 T15272 conj failure,retention
R4394 T15248 T15247 amod subcellular,localization
R4395 T15249 T15250 punct [,25
R4396 T15279 T15280 aux to,translocate
R4397 T15250 T15247 parataxis 25,localization
R4398 T15251 T15250 punct ],25
R4399 T15252 T15247 punct ", ",localization
R4400 T15280 T15278 acl translocate,failure
R4401 T15253 T15254 punct [,27
R4402 T15254 T15247 parataxis 27,localization
R4403 T15255 T15254 punct ],27
R4404 T15281 T15280 prep in,translocate
R4405 T15256 T15247 cc and,localization
R4406 T15257 T15247 conj failure,localization
R4407 T15258 T15259 aux to,translocate
R4408 T15282 T15281 pobj response,in
R4409 T15259 T15257 acl translocate,failure
R4410 T15260 T15259 advmod appropriately,translocate
R4411 T15261 T15259 prep to,translocate
R4412 T15283 T15282 prep to,response
R4413 T15262 T15263 det the,membrane
R4414 T15263 T15261 pobj membrane,to
R4415 T15264 T15263 compound plasma,membrane
R4416 T15265 T15231 punct .,shows
R4417 T15284 T15283 pobj dDAVP,to
R4418 T15285 T15268 auxpass were,proposed
R4419 T15286 T15268 prep as,proposed
R4420 T15287 T15288 det the,mechanism
R4421 T15288 T15286 pobj mechanism,as
R4422 T15289 T15288 prep for,mechanism
R4423 T15290 T15291 amod autosomal,NDI
R4424 T15291 T15289 pobj NDI,for
R4425 T15292 T15291 amod recessive,NDI
R4426 T15293 T15268 punct .,proposed
R4427 T15295 T15296 advmod Here,prove
R4428 T15297 T15296 nsubj we,prove
R4429 T15298 T15296 preconj not,prove
R4430 T15299 T15298 advmod only,not
R4431 T15300 T15301 det this,hypothesis
R4432 T15301 T15296 dobj hypothesis,prove
R4433 T15302 T15296 cc but,prove
R4434 T15303 T15302 advmod also,but
R4435 T15304 T15296 conj establish,prove
R4436 T15305 T15306 det a,model
R4437 T15306 T15304 dobj model,establish
R4438 T15307 T15306 amod useful,model
R4439 T15308 T15306 prep for,model
R4440 T15309 T15310 amod human,NDI
R4441 T15310 T15308 pobj NDI,for
R4442 T15311 T15296 punct .,prove
R4443 T15313 T15314 det This,model
R4444 T15314 T15316 nsubj model,provides
R4445 T15315 T15314 compound mouse,model
R4446 T15317 T15314 prep of,model
R4447 T15318 T15317 pobj NDI,of
R4448 T15319 T15314 prep based,model
R4449 T15320 T15319 prep on,based
R4450 T15321 T15322 det an,allele
R4451 T15322 T15320 pobj allele,on
R4452 T15323 T15322 compound Aqp2,allele
R4453 T15324 T15325 dep that,rescued
R4454 T15325 T15322 relcl rescued,allele
R4455 T15326 T15325 aux can,rescued
R4456 T15327 T15325 auxpass be,rescued
R4457 T15328 T15329 det the,opportunity
R4458 T15329 T15316 dobj opportunity,provides
R4459 T15330 T15331 aux to,test
R4460 T15331 T15329 acl test,opportunity
R4461 T15332 T15331 dobj therapies,test
R4462 T15333 T15332 punct ", ",therapies
R4463 T15334 T15332 prep including,therapies
R4464 T15335 T15336 compound gene,therapy
R4465 T15336 T15334 pobj therapy,including
R4466 T15337 T15332 punct ", ",therapies
R4467 T15338 T15339 dep that,promote
R4468 T15339 T15332 relcl promote,therapies
R4469 T15340 T15339 aux may,promote
R4470 T15341 T15342 amod proper,localization
R4471 T15342 T15339 dobj localization,promote
R4472 T15343 T15342 amod subcellular,localization
R4473 T15344 T15316 punct .,provides
R4474 T15508 T15507 prep of,Generation
R4475 T15509 T15510 compound ENU,mice
R4476 T15510 T15508 pobj mice,of
R4477 T15511 T15507 cc and,Generation
R4478 T15512 T15507 conj housing,Generation
R4479 T15513 T15512 punct .,housing
R4480 T15515 T15516 nmod ENU,mice
R4481 T15516 T15521 nsubjpass mice,generated
R4482 T15517 T15516 amod mutagenized,mice
R4483 T15518 T15516 nmod C57BL,mice
R4484 T15519 T15518 punct /,C57BL
R4485 T15520 T15518 nummod 6,C57BL
R4486 T15522 T15521 auxpass were,generated
R4487 T15523 T15524 mark as,described
R4488 T15524 T15521 advcl described,generated
R4489 T15525 T15526 punct [,19
R4490 T15526 T15521 parataxis 19,generated
R4491 T15527 T15526 punct ],19
R4492 T15528 T15521 punct .,generated
R4493 T15530 T15531 nsubjpass Mice,maintained
R4494 T15532 T15531 auxpass were,maintained
R4495 T15533 T15531 prep by,maintained
R4496 T15534 T15533 pcomp backcrossing,by
R4497 T15535 T15536 amod affected,animals
R4498 T15536 T15534 dobj animals,backcrossing
R4499 T15537 T15534 prep to,backcrossing
R4500 T15538 T15537 pobj C57BL,to
R4501 T15539 T15538 punct /,C57BL
R4502 T15540 T15538 nummod 6,C57BL
R4503 T15541 T15531 cc and,maintained
R4504 T15542 T15531 conj housed,maintained
R4505 T15543 T15542 prep in,housed
R4506 T15544 T15545 det the,Institute
R4507 T15545 T15543 pobj Institute,in
R4508 T15546 T15545 compound Genomics,Institute
R4509 T15547 T15545 prep of,Institute
R4510 T15548 T15549 det the,facility
R4511 T15549 T15547 pobj facility,of
R4512 T15550 T15551 compound Novartis,Foundation
R4513 T15551 T15549 compound Foundation,facility
R4514 T15552 T15551 compound Research,Foundation
R4515 T15553 T15554 compound Specific,Pathogen
R4516 T15554 T15555 compound Pathogen,Free
R4517 T15555 T15549 compound Free,facility
R4518 T15556 T15549 compound animal,facility
R4519 T15557 T15558 punct (,Jolla
R4520 T15558 T15549 parataxis Jolla,facility
R4521 T15559 T15558 compound La,Jolla
R4522 T15560 T15558 punct ", ",Jolla
R4523 T15561 T15558 npadvmod California,Jolla
R4524 T15562 T15558 punct ", ",Jolla
R4525 T15563 T15564 compound United,States
R4526 T15564 T15558 npadvmod States,Jolla
R4527 T15565 T15558 punct ),Jolla
R4528 T15566 T15531 punct .,maintained
R4529 T15568 T15569 det All,procedures
R4530 T15569 T15570 nsubjpass procedures,approved
R4531 T15571 T15570 auxpass were,approved
R4532 T15572 T15570 agent by,approved
R4533 T15573 T15574 det the,Institute
R4534 T15574 T15572 pobj Institute,by
R4535 T15575 T15574 compound Genomics,Institute
R4536 T15576 T15574 prep of,Institute
R4537 T15577 T15578 det the,Committee
R4538 T15578 T15576 pobj Committee,of
R4539 T15579 T15580 nmod Novartis,Foundation
R4540 T15580 T15578 nmod Foundation,Committee
R4541 T15581 T15580 nmod Research,Foundation
R4542 T15582 T15583 nmod Institutional,Animal
R4543 T15583 T15578 nmod Animal,Committee
R4544 T15584 T15583 appos Care,Animal
R4545 T15585 T15584 cc and,Care
R4546 T15586 T15584 conj Use,Care
R4547 T15587 T15570 punct .,approved
R4548 T15901 T15900 punct .,Constructs
R4549 T15903 T15904 det The,sequence
R4550 T15904 T15907 nsubjpass sequence,digested
R4551 T15905 T15904 amod complete,sequence
R4552 T15906 T15904 compound coding,sequence
R4553 T15908 T15904 prep of,sequence
R4554 T15909 T15910 compound mouse,AQP2
R4555 T15910 T15908 pobj AQP2,of
R4556 T15911 T15904 prep from,sequence
R4557 T15912 T15913 det an,clone
R4558 T15913 T15911 pobj clone,from
R4559 T15914 T15913 compound IMAGE,clone
R4560 T15915 T15907 auxpass was,digested
R4561 T15916 T15907 prep from,digested
R4562 T15917 T15918 det the,plasmid
R4563 T15918 T15916 pobj plasmid,from
R4564 T15919 T15918 compound pCMV⋅SPORT6,plasmid
R4565 T15920 T15907 prep with,digested
R4566 T15921 T15920 pobj EcoRI,with
R4567 T15922 T15921 cc and,EcoRI
R4568 T15923 T15921 conj NotI,EcoRI
R4569 T15924 T15907 cc and,digested
R4570 T15925 T15907 conj ligated,digested
R4571 T15926 T15925 prep into,ligated
R4572 T15927 T15926 pobj pcDNA3.1,into
R4573 T15928 T15929 punct (,Invitrogen
R4574 T15929 T15927 parataxis Invitrogen,pcDNA3.1
R4575 T15930 T15929 punct ", ",Invitrogen
R4576 T15931 T15929 npadvmod Carlsbad,Invitrogen
R4577 T15932 T15929 punct ", ",Invitrogen
R4578 T15933 T15929 npadvmod California,Invitrogen
R4579 T15934 T15929 punct ", ",Invitrogen
R4580 T15935 T15936 compound United,States
R4581 T15936 T15929 npadvmod States,Invitrogen
R4582 T15937 T15929 punct ),Invitrogen
R4583 T15938 T15907 punct .,digested
R4584 T15940 T15941 det The,mutation
R4585 T15941 T15943 nsubjpass mutation,introduced
R4586 T15942 T15941 compound F204V,mutation
R4587 T15944 T15943 auxpass was,introduced
R4588 T15945 T15943 prep by,introduced
R4589 T15946 T15947 npadvmod site,directed
R4590 T15947 T15949 amod directed,mutagenesis
R4591 T15948 T15947 punct -,directed
R4592 T15949 T15945 pobj mutagenesis,by
R4593 T15950 T15951 punct (,Stratagene
R4594 T15951 T15949 parataxis Stratagene,mutagenesis
R4595 T15952 T15951 punct ", ",Stratagene
R4596 T15953 T15954 compound La,Jolla
R4597 T15954 T15951 npadvmod Jolla,Stratagene
R4598 T15955 T15951 punct ", ",Stratagene
R4599 T15956 T15951 npadvmod California,Stratagene
R4600 T15957 T15951 punct ", ",Stratagene
R4601 T15958 T15959 compound United,States
R4602 T15959 T15951 npadvmod States,Stratagene
R4603 T15960 T15951 punct ),Stratagene
R4604 T15961 T15943 punct ", ",introduced
R4605 T15962 T15943 advcl using,introduced
R4606 T15963 T15964 det the,oligonucleotide
R4607 T15964 T15962 dobj oligonucleotide,using
R4608 T15965 T15964 compound sense,oligonucleotide
R4609 T15966 T15967 nummod 5,GATGATCACTGGGTCGTCTGGATCGGACCCC
R4610 T15967 T15964 appos GATGATCACTGGGTCGTCTGGATCGGACCCC,oligonucleotide
R4611 T15968 T15966 punct ′,5
R4612 T15969 T15967 punct -,GATGATCACTGGGTCGTCTGGATCGGACCCC
R4613 T15970 T15967 punct -,GATGATCACTGGGTCGTCTGGATCGGACCCC
R4614 T15971 T15967 nummod 3,GATGATCACTGGGTCGTCTGGATCGGACCCC
R4615 T15972 T15967 punct ′,GATGATCACTGGGTCGTCTGGATCGGACCCC
R4616 T15973 T15964 punct ", ",oligonucleotide
R4617 T15974 T15964 cc and,oligonucleotide
R4618 T15975 T15976 amod antisense,oligonucleotide
R4619 T15976 T15964 conj oligonucleotide,oligonucleotide
R4620 T15977 T15978 nummod 5,GGGGTCCGATCCAGACGACCCAGTGATCATC
R4621 T15978 T15976 appos GGGGTCCGATCCAGACGACCCAGTGATCATC,oligonucleotide
R4622 T15979 T15977 punct ′,5
R4623 T15980 T15978 punct -,GGGGTCCGATCCAGACGACCCAGTGATCATC
R4624 T15981 T15978 punct -,GGGGTCCGATCCAGACGACCCAGTGATCATC
R4625 T15982 T15978 nummod 3,GGGGTCCGATCCAGACGACCCAGTGATCATC
R4626 T15983 T15978 punct ′,GGGGTCCGATCCAGACGACCCAGTGATCATC
R4627 T15984 T15943 punct .,introduced
R4628 T15986 T15987 aux To,generate
R4629 T15987 T15988 advcl generate,used
R4630 T15989 T15990 compound GFP,fusions
R4631 T15990 T15987 dobj fusions,generate
R4632 T15991 T15990 prep of,fusions
R4633 T15992 T15991 pobj AQP2,of
R4634 T15993 T15988 punct ", ",used
R4635 T15994 T15995 det the,construct
R4636 T15995 T15988 nsubjpass construct,used
R4637 T15996 T15995 compound pCMV⋅SPORT6,construct
R4638 T15997 T15995 compound AQP2,construct
R4639 T15998 T15988 auxpass was,used
R4640 T15999 T15988 prep in,used
R4641 T16000 T16001 det a,reaction
R4642 T16001 T15999 pobj reaction,in
R4643 T16002 T16001 compound PCR,reaction
R4644 T16003 T16001 prep with,reaction
R4645 T16004 T16005 det the,Sp6
R4646 T16005 T16003 pobj Sp6,with
R4647 T16006 T16005 compound primers,Sp6
R4648 T16007 T16005 cc and,Sp6
R4649 T16008 T16009 nummod 5,GACTGGATCCCGGCCTTGCTGCCGCGCGGCAG
R4650 T16009 T16005 conj GACTGGATCCCGGCCTTGCTGCCGCGCGGCAG,Sp6
R4651 T16010 T16008 punct ′,5
R4652 T16011 T16009 punct -,GACTGGATCCCGGCCTTGCTGCCGCGCGGCAG
R4653 T16012 T16009 punct -,GACTGGATCCCGGCCTTGCTGCCGCGCGGCAG
R4654 T16013 T16009 nummod 3,GACTGGATCCCGGCCTTGCTGCCGCGCGGCAG
R4655 T16014 T16009 punct ′,GACTGGATCCCGGCCTTGCTGCCGCGCGGCAG
R4656 T16015 T16016 aux to,remove
R4657 T16016 T15988 advcl remove,used
R4658 T16017 T16018 det the,codon
R4659 T16018 T16016 dobj codon,remove
R4660 T16019 T16018 compound stop,codon
R4661 T16020 T16018 prep of,codon
R4662 T16021 T16020 pobj AQP2,of
R4663 T16022 T15988 punct .,used
R4664 T16024 T16025 det The,product
R4665 T16025 T16026 nsubjpass product,digested
R4666 T16027 T16026 auxpass was,digested
R4667 T16028 T16026 prep with,digested
R4668 T16029 T16028 pobj KpnI,with
R4669 T16030 T16029 cc and,KpnI
R4670 T16031 T16029 conj BamHI,KpnI
R4671 T16032 T16026 cc and,digested
R4672 T16033 T16026 conj ligated,digested
R4673 T16034 T16033 prep into,ligated
R4674 T16035 T16036 compound pEGFP,N2
R4675 T16036 T16034 pobj N2,into
R4676 T16037 T16036 punct -,N2
R4677 T16038 T16039 punct (,Biosciences
R4678 T16039 T16036 parataxis Biosciences,N2
R4679 T16040 T16039 compound BD,Biosciences
R4680 T16041 T16039 punct ", ",Biosciences
R4681 T16042 T16043 compound San,Diego
R4682 T16043 T16039 npadvmod Diego,Biosciences
R4683 T16044 T16039 punct ", ",Biosciences
R4684 T16045 T16039 npadvmod California,Biosciences
R4685 T16046 T16039 punct ", ",Biosciences
R4686 T16047 T16048 compound United,States
R4687 T16048 T16039 npadvmod States,Biosciences
R4688 T16049 T16039 punct ),Biosciences
R4689 T16050 T16026 punct .,digested
R4690 T16052 T16053 det The,mutation
R4691 T16053 T16055 nsubjpass mutation,introduced
R4692 T16054 T16053 compound F204V,mutation
R4693 T16056 T16055 auxpass was,introduced
R4694 T16057 T16055 advcl using,introduced
R4695 T16058 T16059 det the,oligonucleotides
R4696 T16059 T16057 dobj oligonucleotides,using
R4697 T16060 T16059 amod same,oligonucleotides
R4698 T16061 T16059 amod mutagenic,oligonucleotides
R4699 T16062 T16055 punct .,introduced
R4700 T16399 T16400 compound Cell,culture
R4701 T16401 T16400 cc and,culture
R4702 T16402 T16400 conj generation,culture
R4703 T16403 T16400 prep of,culture
R4704 T16404 T16405 amod stable,lines
R4705 T16405 T16403 pobj lines,of
R4706 T16406 T16405 compound cell,lines
R4707 T16407 T16400 punct .,culture
R4708 T16409 T16410 compound MDCK,cells
R4709 T16410 T16411 nsubjpass cells,cultured
R4710 T16412 T16413 punct (,ATCC
R4711 T16413 T16410 parataxis ATCC,cells
R4712 T16414 T16413 dep CCL,ATCC
R4713 T16415 T16414 punct -,CCL
R4714 T16416 T16414 nummod 34,CCL
R4715 T16417 T16413 punct ;,ATCC
R4716 T16418 T16413 punct ", ",ATCC
R4717 T16419 T16413 npadvmod Manassas,ATCC
R4718 T16420 T16413 punct ", ",ATCC
R4719 T16421 T16413 npadvmod Virginia,ATCC
R4720 T16422 T16413 punct ", ",ATCC
R4721 T16423 T16424 compound United,States
R4722 T16424 T16413 npadvmod States,ATCC
R4723 T16425 T16413 punct ),ATCC
R4724 T16426 T16411 auxpass were,cultured
R4725 T16427 T16411 prep in,cultured
R4726 T16428 T16427 pobj DMEM,in
R4727 T16429 T16430 punct (,Aldrich
R4728 T16430 T16428 parataxis Aldrich,DMEM
R4729 T16431 T16430 compound Sigma,Aldrich
R4730 T16432 T16430 punct -,Aldrich
R4731 T16433 T16430 punct ", ",Aldrich
R4732 T16434 T16435 compound St.,Louis
R4733 T16435 T16430 npadvmod Louis,Aldrich
R4734 T16436 T16430 punct ", ",Aldrich
R4735 T16437 T16430 npadvmod Missouri,Aldrich
R4736 T16438 T16430 punct ", ",Aldrich
R4737 T16439 T16440 compound United,States
R4738 T16440 T16430 npadvmod States,Aldrich
R4739 T16441 T16430 punct ),Aldrich
R4740 T16442 T16411 dep supplemented,cultured
R4741 T16443 T16442 prep with,supplemented
R4742 T16444 T16445 nummod 10,%
R4743 T16445 T16446 compound %,FBS
R4744 T16446 T16443 pobj FBS,with
R4745 T16447 T16448 punct (,Aldrich
R4746 T16448 T16446 parataxis Aldrich,FBS
R4747 T16449 T16448 compound Sigma,Aldrich
R4748 T16450 T16448 punct -,Aldrich
R4749 T16451 T16448 punct ),Aldrich
R4750 T16452 T16446 punct ", ",FBS
R4751 T16453 T16454 nummod 100,U
R4752 T16454 T16446 conj U,FBS
R4753 T16455 T16456 punct /,ml
R4754 T16456 T16454 prep ml,U
R4755 T16457 T16454 prep of,U
R4756 T16458 T16457 pobj penicillin,of
R4757 T16459 T16454 punct ", ",U
R4758 T16460 T16454 cc and,U
R4759 T16461 T16462 nummod 100,μg
R4760 T16462 T16454 conj μg,U
R4761 T16463 T16464 punct /,ml
R4762 T16464 T16462 prep ml,μg
R4763 T16465 T16462 prep of,μg
R4764 T16466 T16465 pobj streptomycin,of
R4765 T16467 T16446 prep at,FBS
R4766 T16468 T16469 nummod 37,°C
R4767 T16469 T16467 pobj °C,at
R4768 T16470 T16446 prep in,FBS
R4769 T16471 T16472 nummod 5,%
R4770 T16472 T16473 compound %,CO2
R4771 T16473 T16470 pobj CO2,in
R4772 T16474 T16411 punct .,cultured
R4773 T16476 T16477 aux To,generate
R4774 T16477 T16478 advcl generate,transfected
R4775 T16479 T16480 amod stable,lines
R4776 T16480 T16477 dobj lines,generate
R4777 T16481 T16480 compound MDCK,lines
R4778 T16482 T16480 compound cell,lines
R4779 T16483 T16478 punct ", ",transfected
R4780 T16484 T16478 nsubjpass cells,transfected
R4781 T16485 T16478 auxpass were,transfected
R4782 T16486 T16478 advcl using,transfected
R4783 T16487 T16486 dobj Lipofectamine,using
R4784 T16488 T16487 nummod 2000,Lipofectamine
R4785 T16489 T16490 punct (,Invitrogen
R4786 T16490 T16487 parataxis Invitrogen,Lipofectamine
R4787 T16491 T16490 punct ),Invitrogen
R4788 T16492 T16487 cc and,Lipofectamine
R4789 T16493 T16494 det the,constructs
R4790 T16494 T16487 conj constructs,Lipofectamine
R4791 T16495 T16494 compound pcDNA3.1,constructs
R4792 T16496 T16494 compound expression,constructs
R4793 T16497 T16494 punct (,constructs
R4794 T16498 T16494 acl containing,constructs
R4795 T16499 T16500 amod wild,type
R4796 T16500 T16502 compound type,AQP2
R4797 T16501 T16500 punct -,type
R4798 T16502 T16498 dobj AQP2,containing
R4799 T16503 T16502 punct ", ",AQP2
R4800 T16504 T16505 compound AQP2,F204V
R4801 T16505 T16502 conj F204V,AQP2
R4802 T16506 T16505 punct -,F204V
R4803 T16507 T16505 punct ", ",F204V
R4804 T16508 T16505 cc or,F204V
R4805 T16509 T16510 det no,insert
R4806 T16510 T16505 conj insert,F204V
R4807 T16511 T16494 punct ),constructs
R4808 T16512 T16478 cc and,transfected
R4809 T16513 T16478 conj selected,transfected
R4810 T16514 T16513 prep with,selected
R4811 T16515 T16516 nummod 900,μg
R4812 T16516 T16517 nmod μg,G418
R4813 T16517 T16514 pobj G418,with
R4814 T16518 T16519 punct /,ml
R4815 T16519 T16516 prep ml,μg
R4816 T16520 T16521 punct (,Aldrich
R4817 T16521 T16517 parataxis Aldrich,G418
R4818 T16522 T16521 compound Sigma,Aldrich
R4819 T16523 T16521 punct -,Aldrich
R4820 T16524 T16521 punct ),Aldrich
R4821 T16525 T16478 punct .,transfected
R4822 T16527 T16528 amod Individual,colonies
R4823 T16528 T16529 nsubjpass colonies,expanded
R4824 T16530 T16529 auxpass were,expanded
R4825 T16531 T16532 nummod 14,d
R4826 T16532 T16533 npadvmod d,later
R4827 T16533 T16529 advmod later,expanded
R4828 T16534 T16529 punct .,expanded
R4829 T16536 T16537 prep For,added
R4830 T16538 T16539 det the,duration
R4831 T16539 T16536 pobj duration,For
R4832 T16540 T16539 prep of,duration
R4833 T16541 T16542 det these,experiments
R4834 T16542 T16540 pobj experiments,of
R4835 T16543 T16537 punct ", ",added
R4836 T16544 T16545 det the,antibiotic
R4837 T16545 T16537 nsubjpass antibiotic,added
R4838 T16546 T16537 auxpass was,added
R4839 T16547 T16537 advmod continually,added
R4840 T16548 T16537 prep to,added
R4841 T16549 T16550 det the,media
R4842 T16550 T16548 pobj media,to
R4843 T16551 T16537 punct .,added
R4844 T16553 T16554 amod Transient,transfections
R4845 T16554 T16556 nsubjpass transfections,carried
R4846 T16555 T16554 compound GFP,transfections
R4847 T16557 T16556 auxpass were,carried
R4848 T16558 T16556 prt out,carried
R4849 T16559 T16556 prep in,carried
R4850 T16560 T16561 amod subconfluent,lines
R4851 T16561 T16559 pobj lines,in
R4852 T16562 T16561 amod stable,lines
R4853 T16563 T16561 compound cells,lines
R4854 T16564 T16556 advmod also,carried
R4855 T16565 T16556 advcl using,carried
R4856 T16566 T16565 dobj Lipofectamine,using
R4857 T16567 T16566 nummod 2000,Lipofectamine
R4858 T16568 T16556 punct .,carried
R4859 T16677 T16676 prep of,Sequencing
R4860 T16678 T16677 pobj Aqp2,of
R4861 T16679 T16676 cc and,Sequencing
R4862 T16680 T16676 conj genotyping,Sequencing
R4863 T16681 T16680 prep of,genotyping
R4864 T16682 T16681 pobj mice,of
R4865 T16683 T16680 punct .,genotyping
R4866 T16685 T16686 det All,exons
R4867 T16686 T16687 nsubjpass exons,amplified
R4868 T16688 T16686 prep of,exons
R4869 T16689 T16688 pobj Aqp2,of
R4870 T16690 T16687 auxpass were,amplified
R4871 T16691 T16687 prep from,amplified
R4872 T16692 T16693 nmod mouse,DNA
R4873 T16693 T16691 pobj DNA,from
R4874 T16694 T16693 amod genomic,DNA
R4875 T16695 T16687 cc and,amplified
R4876 T16696 T16687 conj sequenced,amplified
R4877 T16697 T16687 punct .,amplified
R4878 T16699 T16700 prep For,amplified
R4879 T16701 T16699 pobj genotyping,For
R4880 T16702 T16700 punct ", ",amplified
R4881 T16703 T16700 nsubjpass exon,amplified
R4882 T16704 T16703 nummod 4,exon
R4883 T16705 T16700 auxpass was,amplified
R4884 T16706 T16700 advcl using,amplified
R4885 T16707 T16708 det the,primers
R4886 T16708 T16706 dobj primers,using
R4887 T16709 T16710 nummod 5,TCAGAACTTGCCCACTAGCC
R4888 T16710 T16708 appos TCAGAACTTGCCCACTAGCC,primers
R4889 T16711 T16709 punct ′,5
R4890 T16712 T16710 punct -,TCAGAACTTGCCCACTAGCC
R4891 T16713 T16710 punct -,TCAGAACTTGCCCACTAGCC
R4892 T16714 T16710 nummod 3,TCAGAACTTGCCCACTAGCC
R4893 T16715 T16710 punct ′,TCAGAACTTGCCCACTAGCC
R4894 T16716 T16710 cc and,TCAGAACTTGCCCACTAGCC
R4895 T16717 T16718 nummod 5,TGTAGAGGAGGGAACCGATG
R4896 T16718 T16710 conj TGTAGAGGAGGGAACCGATG,TCAGAACTTGCCCACTAGCC
R4897 T16719 T16717 punct ′,5
R4898 T16720 T16718 punct -,TGTAGAGGAGGGAACCGATG
R4899 T16721 T16718 punct -,TGTAGAGGAGGGAACCGATG
R4900 T16722 T16718 nummod 3,TGTAGAGGAGGGAACCGATG
R4901 T16723 T16718 punct ′,TGTAGAGGAGGGAACCGATG
R4902 T16724 T16700 punct .,amplified
R4903 T16959 T16960 compound Urine,measurements
R4904 T16961 T16960 punct .,measurements
R4905 T16963 T16964 amod Total,output
R4906 T16964 T16966 nsubjpass output,measured
R4907 T16965 T16964 compound urine,output
R4908 T16967 T16966 auxpass was,measured
R4909 T16968 T16966 prep by,measured
R4910 T16969 T16970 advmod separately,housing
R4911 T16970 T16968 pcomp housing,by
R4912 T16971 T16972 amod adult,mice
R4913 T16972 T16970 dobj mice,housing
R4914 T16973 T16970 prep in,housing
R4915 T16974 T16975 nmod Nalgene,Cages
R4916 T16975 T16973 pobj Cages,in
R4917 T16976 T16975 amod Metabolic,Cages
R4918 T16977 T16978 punct (,Minimitter
R4919 T16978 T16975 parataxis Minimitter,Cages
R4920 T16979 T16978 punct ", ",Minimitter
R4921 T16980 T16978 npadvmod Bend,Minimitter
R4922 T16981 T16978 punct ", ",Minimitter
R4923 T16982 T16978 npadvmod Oregon,Minimitter
R4924 T16983 T16978 punct ", ",Minimitter
R4925 T16984 T16985 compound United,States
R4926 T16985 T16978 npadvmod States,Minimitter
R4927 T16986 T16978 punct ),Minimitter
R4928 T16987 T16970 prep for,housing
R4929 T16988 T16989 quantmod 2,3
R4930 T16989 T16991 nummod 3,d
R4931 T16990 T16989 punct –,3
R4932 T16991 T16987 pobj d,for
R4933 T16992 T16970 cc and,housing
R4934 T16993 T16970 conj collecting,housing
R4935 T16994 T16993 dobj urine,collecting
R4936 T16995 T16996 det every,period
R4937 T16996 T16993 npadvmod period,collecting
R4938 T16997 T16998 nummod 24,h
R4939 T16998 T16996 compound h,period
R4940 T16999 T16966 punct .,measured
R4941 T17001 T17002 compound Urine,osmolalities
R4942 T17002 T17003 nsubjpass osmolalities,determined
R4943 T17004 T17003 auxpass were,determined
R4944 T17005 T17003 advcl using,determined
R4945 T17006 T17007 det an,Osmometer
R4946 T17007 T17005 dobj Osmometer,using
R4947 T17008 T17009 punct (,Systems
R4948 T17009 T17007 parataxis Systems,Osmometer
R4949 T17010 T17009 dep Osmette,Systems
R4950 T17011 T17010 nummod 5004,Osmette
R4951 T17012 T17009 punct ;,Systems
R4952 T17013 T17009 compound Precision,Systems
R4953 T17014 T17009 punct ", ",Systems
R4954 T17015 T17009 npadvmod Natick,Systems
R4955 T17016 T17009 punct ", ",Systems
R4956 T17017 T17009 npadvmod Massachusetts,Systems
R4957 T17018 T17009 punct ", ",Systems
R4958 T17019 T17020 compound United,States
R4959 T17020 T17009 npadvmod States,Systems
R4960 T17021 T17009 punct ),Systems
R4961 T17022 T17003 punct .,determined
R4962 T17024 T17025 npadvmod Urine,concentrating
R4963 T17025 T17026 amod concentrating,experiments
R4964 T17026 T17027 nsubjpass experiments,carried
R4965 T17028 T17027 auxpass were,carried
R4966 T17029 T17027 prt out,carried
R4967 T17030 T17027 prep by,carried
R4968 T17031 T17032 amod intraperitoneal,injection
R4969 T17032 T17030 pobj injection,by
R4970 T17033 T17032 prep of,injection
R4971 T17034 T17033 pobj dDAVP,of
R4972 T17035 T17036 punct (,μg
R4973 T17036 T17034 parataxis μg,dDAVP
R4974 T17037 T17036 nummod 0.4,μg
R4975 T17038 T17039 punct /,kg
R4976 T17039 T17036 prep kg,μg
R4977 T17040 T17036 punct ),μg
R4978 T17041 T17027 punct .,carried
R4979 T17043 T17044 nsubjpass Mice,injected
R4980 T17045 T17044 auxpass were,injected
R4981 T17046 T17044 advmod twice,injected
R4982 T17047 T17044 prep with,injected
R4983 T17048 T17047 pobj dDAVP,with
R4984 T17049 T17044 punct ", ",injected
R4985 T17050 T17051 advmod once,at
R4986 T17051 T17044 prep at,injected
R4987 T17052 T17051 pobj time,at
R4988 T17053 T17052 nummod 0,time
R4989 T17054 T17051 cc and,at
R4990 T17055 T17056 advmod again,at
R4991 T17056 T17051 conj at,at
R4992 T17057 T17058 nummod 30,min
R4993 T17058 T17056 pobj min,at
R4994 T17059 T17044 punct .,injected
R4995 T17061 T17062 nsubjpass Urine,collected
R4996 T17063 T17062 auxpass was,collected
R4997 T17064 T17062 prep at,collected
R4998 T17065 T17066 det the,start
R4999 T17066 T17064 pobj start,at
R5000 T17067 T17066 prep of,start
R5001 T17068 T17069 det the,experiment
R5002 T17069 T17067 pobj experiment,of
R5003 T17070 T17064 cc and,at
R5004 T17071 T17072 nummod 30,min
R5005 T17072 T17073 npadvmod min,after
R5006 T17073 T17064 conj after,at
R5007 T17074 T17075 det the,injection
R5008 T17075 T17073 pobj injection,after
R5009 T17076 T17075 amod second,injection
R5010 T17077 T17062 punct .,collected
R5011 T17265 T17266 compound Kidney,membrane
R5012 T17266 T17267 compound membrane,preparation
R5013 T17268 T17267 punct .,preparation
R5014 T17270 T17271 amod Whole,kidneys
R5015 T17271 T17273 nsubjpass kidneys,homogenized
R5016 T17272 T17271 compound mouse,kidneys
R5017 T17274 T17273 auxpass were,homogenized
R5018 T17275 T17273 prep in,homogenized
R5019 T17276 T17277 nummod 10,mM
R5020 T17277 T17278 compound mM,Tris
R5021 T17278 T17275 pobj Tris,in
R5022 T17279 T17278 punct (,Tris
R5023 T17280 T17278 npadvmod pH,Tris
R5024 T17281 T17280 nummod 7.4,pH
R5025 T17282 T17278 punct ),Tris
R5026 T17283 T17278 punct ", ",Tris
R5027 T17284 T17285 nummod 350,mM
R5028 T17285 T17286 compound mM,sucrose
R5029 T17286 T17278 conj sucrose,Tris
R5030 T17287 T17286 punct ", ",sucrose
R5031 T17288 T17286 cc and,sucrose
R5032 T17289 T17290 nummod 5,mM
R5033 T17290 T17291 compound mM,EDTA
R5034 T17291 T17286 conj EDTA,sucrose
R5035 T17292 T17291 acl containing,EDTA
R5036 T17293 T17294 compound protease,inhibitors
R5037 T17294 T17292 dobj inhibitors,containing
R5038 T17295 T17296 punct (,P
R5039 T17296 T17294 parataxis P,inhibitors
R5040 T17297 T17298 compound Sigma,Aldrich
R5041 T17298 T17296 dep Aldrich,P
R5042 T17299 T17298 punct -,Aldrich
R5043 T17300 T17296 punct ", ",P
R5044 T17301 T17296 punct #,P
R5045 T17302 T17296 punct -,P
R5046 T17303 T17296 nummod 8340,P
R5047 T17304 T17296 punct ),P
R5048 T17305 T17273 prep in,homogenized
R5049 T17306 T17307 det a,homogenizer
R5050 T17307 T17305 pobj homogenizer,in
R5051 T17308 T17309 compound Potter,Elvehjem
R5052 T17309 T17307 compound Elvehjem,homogenizer
R5053 T17310 T17309 punct -,Elvehjem
R5054 T17311 T17273 punct .,homogenized
R5055 T17313 T17314 det The,homogenate
R5056 T17314 T17315 nsubjpass homogenate,centrifuged
R5057 T17316 T17315 auxpass was,centrifuged
R5058 T17317 T17315 prep at,centrifuged
R5059 T17318 T17319 nummod "2,000",g
R5060 T17319 T17317 pobj g,at
R5061 T17320 T17315 prep for,centrifuged
R5062 T17321 T17322 nummod 10,min
R5063 T17322 T17320 pobj min,for
R5064 T17323 T17315 cc and,centrifuged
R5065 T17324 T17325 det the,supernatant
R5066 T17325 T17326 nsubjpass supernatant,subjected
R5067 T17326 T17315 conj subjected,centrifuged
R5068 T17327 T17326 auxpass was,subjected
R5069 T17328 T17326 prep to,subjected
R5070 T17329 T17328 pobj ultracentrifugation,to
R5071 T17330 T17329 prep at,ultracentrifugation
R5072 T17331 T17332 nummod "100,000",g
R5073 T17332 T17330 pobj g,at
R5074 T17333 T17326 prep for,subjected
R5075 T17334 T17335 nummod 1,h
R5076 T17335 T17333 pobj h,for
R5077 T17336 T17326 prep at,subjected
R5078 T17337 T17338 nummod 4,°C
R5079 T17338 T17336 pobj °C,at
R5080 T17339 T17326 punct .,subjected
R5081 T17341 T17342 amod Pelleted,membranes
R5082 T17342 T17343 nsubjpass membranes,resuspended
R5083 T17344 T17343 auxpass were,resuspended
R5084 T17345 T17343 prep in,resuspended
R5085 T17346 T17347 det the,buffer
R5086 T17347 T17345 pobj buffer,in
R5087 T17348 T17347 amod same,buffer
R5088 T17349 T17343 punct ", ",resuspended
R5089 T17350 T17343 cc and,resuspended
R5090 T17351 T17352 compound protein,concentration
R5091 T17352 T17353 nsubjpass concentration,determined
R5092 T17353 T17343 conj determined,resuspended
R5093 T17354 T17353 auxpass was,determined
R5094 T17355 T17353 prep by,determined
R5095 T17356 T17357 compound Bradford,assay
R5096 T17357 T17355 pobj assay,by
R5097 T17358 T17353 punct .,determined
R5098 T17615 T17614 punct .,Immunoblotting
R5099 T17617 T17618 compound Kidney,membrane
R5100 T17618 T17619 compound membrane,fractions
R5101 T17619 T17620 nsubjpass fractions,resolved
R5102 T17621 T17622 punct (,μg
R5103 T17622 T17619 parataxis μg,fractions
R5104 T17623 T17622 nummod 60,μg
R5105 T17624 T17622 punct ),μg
R5106 T17625 T17620 auxpass were,resolved
R5107 T17626 T17620 prep on,resolved
R5108 T17627 T17628 det a,gel
R5109 T17628 T17626 pobj gel,on
R5110 T17629 T17630 nummod 12,%
R5111 T17630 T17628 compound %,gel
R5112 T17631 T17632 compound SDS,polyacrylamide
R5113 T17632 T17628 compound polyacrylamide,gel
R5114 T17633 T17632 punct -,polyacrylamide
R5115 T17634 T17620 cc and,resolved
R5116 T17635 T17620 conj transferred,resolved
R5117 T17636 T17635 prep to,transferred
R5118 T17637 T17638 det a,membrane
R5119 T17638 T17636 pobj membrane,to
R5120 T17639 T17638 compound nitrocellulose,membrane
R5121 T17640 T17620 punct .,resolved
R5122 T17642 T17643 nsubjpass Membranes,blocked
R5123 T17644 T17643 auxpass were,blocked
R5124 T17645 T17643 prep in,blocked
R5125 T17646 T17647 nummod 5,%
R5126 T17647 T17648 nmod %,milk
R5127 T17648 T17645 pobj milk,in
R5128 T17649 T17648 amod nonfat,milk
R5129 T17650 T17648 prep in,milk
R5130 T17651 T17652 npadvmod Tris,buffered
R5131 T17652 T17654 amod buffered,saline
R5132 T17653 T17652 punct -,buffered
R5133 T17654 T17650 pobj saline,in
R5134 T17655 T17654 prep with,saline
R5135 T17656 T17657 nummod 0.05,%
R5136 T17657 T17658 compound %,Tween
R5137 T17658 T17655 pobj Tween,with
R5138 T17659 T17658 nummod 20,Tween
R5139 T17660 T17661 punct (,TBST
R5140 T17661 T17658 parataxis TBST,Tween
R5141 T17662 T17661 punct ),TBST
R5142 T17663 T17643 punct ", ",blocked
R5143 T17664 T17643 advcl followed,blocked
R5144 T17665 T17664 agent by,followed
R5145 T17666 T17667 det an,incubation
R5146 T17667 T17665 pobj incubation,by
R5147 T17668 T17667 amod overnight,incubation
R5148 T17669 T17667 punct (,incubation
R5149 T17670 T17667 prep at,incubation
R5150 T17671 T17672 nummod 4,°C
R5151 T17672 T17670 pobj °C,at
R5152 T17673 T17667 punct ),incubation
R5153 T17674 T17667 prep with,incubation
R5154 T17675 T17676 nmod AQP2,antibody
R5155 T17676 T17674 pobj antibody,with
R5156 T17677 T17676 amod polyclonal,antibody
R5157 T17678 T17679 punct (,sc
R5158 T17679 T17676 parataxis sc,antibody
R5159 T17680 T17681 compound Santa,Cruz
R5160 T17681 T17682 compound Cruz,Biotechnology
R5161 T17682 T17679 dep Biotechnology,sc
R5162 T17683 T17682 punct ", ",Biotechnology
R5163 T17684 T17685 compound Santa,Cruz
R5164 T17685 T17682 npadvmod Cruz,Biotechnology
R5165 T17686 T17682 punct ", ",Biotechnology
R5166 T17687 T17682 npadvmod California,Biotechnology
R5167 T17688 T17682 punct ", ",Biotechnology
R5168 T17689 T17690 compound United,States
R5169 T17690 T17682 npadvmod States,Biotechnology
R5170 T17691 T17679 punct ;,sc
R5171 T17692 T17679 punct #,sc
R5172 T17693 T17679 punct -,sc
R5173 T17694 T17679 nummod 9882,sc
R5174 T17695 T17679 punct ),sc
R5175 T17696 T17643 punct .,blocked
R5176 T17698 T17699 nsubjpass Membranes,washed
R5177 T17700 T17699 auxpass were,washed
R5178 T17701 T17699 prep in,washed
R5179 T17702 T17701 pobj TBST,in
R5180 T17703 T17704 advmod then,incubated
R5181 T17704 T17699 dep incubated,washed
R5182 T17705 T17704 prep with,incubated
R5183 T17706 T17707 npadvmod HRP,conjugated
R5184 T17707 T17709 amod conjugated,antibody
R5185 T17708 T17707 punct -,conjugated
R5186 T17709 T17705 pobj antibody,with
R5187 T17710 T17709 nmod donkey,antibody
R5188 T17711 T17709 amod anti-goat,antibody
R5189 T17712 T17699 punct .,washed
R5190 T17714 T17715 nsubjpass Membranes,washed
R5191 T17716 T17715 auxpass were,washed
R5192 T17717 T17715 advmod further,washed
R5193 T17718 T17715 prep in,washed
R5194 T17719 T17718 pobj TBST,in
R5195 T17720 T17715 cc and,washed
R5196 T17721 T17722 nsubjpass bands,visualized
R5197 T17722 T17715 conj visualized,washed
R5198 T17723 T17722 auxpass were,visualized
R5199 T17724 T17722 advcl using,visualized
R5200 T17725 T17726 compound ECL,reagent
R5201 T17726 T17724 dobj reagent,using
R5202 T17727 T17728 punct (,Biosciences
R5203 T17728 T17726 parataxis Biosciences,reagent
R5204 T17729 T17728 compound Amersham,Biosciences
R5205 T17730 T17728 punct ", ",Biosciences
R5206 T17731 T17732 compound Little,Chalfont
R5207 T17732 T17728 npadvmod Chalfont,Biosciences
R5208 T17733 T17728 punct ", ",Biosciences
R5209 T17734 T17735 compound United,Kingdom
R5210 T17735 T17728 npadvmod Kingdom,Biosciences
R5211 T17736 T17728 punct ),Biosciences
R5212 T17737 T17722 punct .,visualized
R5213 T17905 T17906 compound Endoglycosidase,digestion
R5214 T17907 T17906 punct .,digestion
R5215 T17909 T17910 compound Kidney,membranes
R5216 T17910 T17911 nsubjpass membranes,incubated
R5217 T17912 T17913 punct (,μg
R5218 T17913 T17910 parataxis μg,membranes
R5219 T17914 T17913 nummod 60,μg
R5220 T17915 T17913 punct ),μg
R5221 T17916 T17911 auxpass were,incubated
R5222 T17917 T17911 prep in,incubated
R5223 T17918 T17919 nummod 50,mM
R5224 T17919 T17920 compound mM,phosphate
R5225 T17920 T17917 pobj phosphate,in
R5226 T17921 T17920 compound sodium,phosphate
R5227 T17922 T17920 punct (,phosphate
R5228 T17923 T17920 npadvmod pH,phosphate
R5229 T17924 T17923 nummod 5.5,pH
R5230 T17925 T17920 punct ),phosphate
R5231 T17926 T17920 punct ", ",phosphate
R5232 T17927 T17928 nummod 0.1,%
R5233 T17928 T17929 compound %,SDS
R5234 T17929 T17920 appos SDS,phosphate
R5235 T17930 T17929 punct ", ",SDS
R5236 T17931 T17929 cc and,SDS
R5237 T17932 T17933 nummod 50,mM
R5238 T17933 T17934 compound mM,mercaptoethanol
R5239 T17934 T17929 conj mercaptoethanol,SDS
R5240 T17935 T17934 compound β,mercaptoethanol
R5241 T17936 T17934 punct -,mercaptoethanol
R5242 T17937 T17911 punct ", ",incubated
R5243 T17938 T17911 dep heated,incubated
R5244 T17939 T17938 prep to,heated
R5245 T17940 T17941 nummod 100,°C
R5246 T17941 T17939 pobj °C,to
R5247 T17942 T17938 prep for,heated
R5248 T17943 T17944 nummod 5,min
R5249 T17944 T17942 pobj min,for
R5250 T17945 T17911 punct ", ",incubated
R5251 T17946 T17947 advmod then,cooled
R5252 T17947 T17911 dep cooled,incubated
R5253 T17948 T17911 punct .,incubated
R5254 T17950 T17951 compound Endoglycosidase,H
R5255 T17951 T17952 nsubjpass H,added
R5256 T17953 T17954 punct (,Aldrich
R5257 T17954 T17951 parataxis Aldrich,H
R5258 T17955 T17956 nummod 0.01,units
R5259 T17956 T17954 dep units,Aldrich
R5260 T17957 T17954 punct ;,Aldrich
R5261 T17958 T17954 compound Sigma,Aldrich
R5262 T17959 T17954 punct -,Aldrich
R5263 T17960 T17954 punct ),Aldrich
R5264 T17961 T17952 auxpass was,added
R5265 T17962 T17952 cc and,added
R5266 T17963 T17952 conj incubated,added
R5267 T17964 T17963 prep at,incubated
R5268 T17965 T17966 nummod 37,°C
R5269 T17966 T17964 pobj °C,at
R5270 T17967 T17963 prep for,incubated
R5271 T17968 T17969 nummod 2,h
R5272 T17969 T17967 pobj h,for
R5273 T17970 T17952 punct .,added
R5274 T17972 T17973 det The,reaction
R5275 T17973 T17974 nsubjpass reaction,stopped
R5276 T17975 T17974 auxpass was,stopped
R5277 T17976 T17974 prep by,stopped
R5278 T17977 T17976 pcomp boiling,by
R5279 T17978 T17979 det the,samples
R5280 T17979 T17977 dobj samples,boiling
R5281 T17980 T17977 prep in,boiling
R5282 T17981 T17982 compound Laemmli,buffer
R5283 T17982 T17980 pobj buffer,in
R5284 T17983 T17974 punct .,stopped
R5285 T17985 T17986 amod Total,reactants
R5286 T17986 T17987 nsubjpass reactants,immunoblotted
R5287 T17988 T17987 auxpass were,immunoblotted
R5288 T17989 T17990 mark as,described
R5289 T17990 T17987 advcl described,immunoblotted
R5290 T17991 T17990 advmod above,described
R5291 T17992 T17987 punct .,immunoblotted
R5292 T18784 T18783 cc and,Coimmunoprecipitation
R5293 T18785 T18783 conj biotinylation,Coimmunoprecipitation
R5294 T18786 T18783 prep in,Coimmunoprecipitation
R5295 T18787 T18788 compound MDCK,cells
R5296 T18788 T18786 pobj cells,in
R5297 T18789 T18783 punct .,Coimmunoprecipitation
R5298 T18791 T18792 compound MDCK,cells
R5299 T18792 T18793 nsubjpass cells,transfected
R5300 T18794 T18795 advmod stably,expressing
R5301 T18795 T18792 acl expressing,cells
R5302 T18796 T18797 amod wild,type
R5303 T18797 T18799 compound type,AQP2
R5304 T18798 T18797 punct -,type
R5305 T18799 T18795 dobj AQP2,expressing
R5306 T18800 T18799 punct (,AQP2
R5307 T18801 T18799 acl grown,AQP2
R5308 T18802 T18801 prep on,grown
R5309 T18803 T18804 nummod 10,cm
R5310 T18804 T18806 compound cm,plates
R5311 T18805 T18804 punct -,cm
R5312 T18806 T18802 pobj plates,on
R5313 T18807 T18795 punct ),expressing
R5314 T18808 T18793 auxpass were,transfected
R5315 T18809 T18793 prep with,transfected
R5316 T18810 T18811 nmod pEGFP,type
R5317 T18811 T18815 compound type,AQP2
R5318 T18812 T18811 punct -,type
R5319 T18813 T18811 amod wild,type
R5320 T18814 T18811 punct -,type
R5321 T18815 T18809 pobj AQP2,with
R5322 T18816 T18815 punct ", ",AQP2
R5323 T18817 T18818 compound pEGFP,F204V
R5324 T18818 T18815 conj F204V,AQP2
R5325 T18819 T18818 punct -,F204V
R5326 T18820 T18818 compound AQP2,F204V
R5327 T18821 T18818 punct -,F204V
R5328 T18822 T18818 punct ", ",F204V
R5329 T18823 T18818 cc or,F204V
R5330 T18824 T18818 conj vector,F204V
R5331 T18825 T18824 advmod alone,vector
R5332 T18826 T18793 punct .,transfected
R5333 T18828 T18829 det The,cells
R5334 T18829 T18830 nsubjpass cells,homogenized
R5335 T18831 T18830 auxpass were,homogenized
R5336 T18832 T18830 prep in,homogenized
R5337 T18833 T18834 nummod 10,mM
R5338 T18834 T18835 compound mM,Tris
R5339 T18835 T18832 pobj Tris,in
R5340 T18836 T18835 punct (,Tris
R5341 T18837 T18835 npadvmod pH,Tris
R5342 T18838 T18837 nummod 7.4,pH
R5343 T18839 T18835 punct ),Tris
R5344 T18840 T18835 punct ", ",Tris
R5345 T18841 T18842 nummod 1,mM
R5346 T18842 T18843 compound mM,EDTA
R5347 T18843 T18835 conj EDTA,Tris
R5348 T18844 T18843 punct ", ",EDTA
R5349 T18845 T18843 cc and,EDTA
R5350 T18846 T18847 nummod 250,mM
R5351 T18847 T18848 compound mM,sucrose
R5352 T18848 T18843 conj sucrose,EDTA
R5353 T18849 T18850 nummod 40,h
R5354 T18850 T18851 npadvmod h,later
R5355 T18851 T18830 advmod later,homogenized
R5356 T18852 T18830 punct .,homogenized
R5357 T18854 T18855 det The,supernatant
R5358 T18855 T18857 nsubjpass supernatant,centrifuged
R5359 T18856 T18855 amod clarified,supernatant
R5360 T18858 T18857 auxpass was,centrifuged
R5361 T18859 T18857 prep at,centrifuged
R5362 T18860 T18861 nummod "200,000",g
R5363 T18861 T18859 pobj g,at
R5364 T18862 T18857 prep for,centrifuged
R5365 T18863 T18864 nummod 30,min
R5366 T18864 T18862 pobj min,for
R5367 T18865 T18857 punct .,centrifuged
R5368 T18867 T18868 amod Pelleted,membranes
R5369 T18868 T18869 nsubjpass membranes,incubated
R5370 T18870 T18869 auxpass were,incubated
R5371 T18871 T18869 aux resuspended,incubated
R5372 T18872 T18869 prep in,incubated
R5373 T18873 T18874 det the,buffer
R5374 T18874 T18872 pobj buffer,in
R5375 T18875 T18874 amod same,buffer
R5376 T18876 T18874 prep but,buffer
R5377 T18877 T18876 pcomp containing,but
R5378 T18878 T18879 nummod 4,%
R5379 T18879 T18880 compound %,deoxycholate
R5380 T18880 T18877 dobj deoxycholate,containing
R5381 T18881 T18880 compound sodium,deoxycholate
R5382 T18882 T18869 cc and,incubated
R5383 T18883 T18869 prep at,incubated
R5384 T18884 T18885 nummod 37,°C
R5385 T18885 T18883 pobj °C,at
R5386 T18886 T18869 prep for,incubated
R5387 T18887 T18888 nummod 1,h
R5388 T18888 T18886 pobj h,for
R5389 T18889 T18869 punct .,incubated
R5390 T18891 T18892 prep From,removed
R5391 T18893 T18894 det the,membranes
R5392 T18894 T18891 pobj membranes,From
R5393 T18895 T18894 amod dissolved,membranes
R5394 T18896 T18892 punct ", ",removed
R5395 T18897 T18898 det a,sample
R5396 T18898 T18892 nsubjpass sample,removed
R5397 T18899 T18900 nummod 30,μl
R5398 T18900 T18898 compound μl,sample
R5399 T18901 T18892 auxpass was,removed
R5400 T18902 T18892 cc and,removed
R5401 T18903 T18892 conj used,removed
R5402 T18904 T18903 prep as,used
R5403 T18905 T18906 det the,fraction
R5404 T18906 T18904 pobj fraction,as
R5405 T18907 T18906 amod total,fraction
R5406 T18908 T18906 compound membrane,fraction
R5407 T18909 T18892 punct .,removed
R5408 T18911 T18912 det The,membranes
R5409 T18912 T18914 nsubjpass membranes,diluted
R5410 T18913 T18912 amod remaining,membranes
R5411 T18915 T18914 auxpass were,diluted
R5412 T18916 T18914 prep with,diluted
R5413 T18917 T18918 nummod 600,μl
R5414 T18918 T18916 pobj μl,with
R5415 T18919 T18918 prep of,μl
R5416 T18920 T18921 det the,buffer
R5417 T18921 T18919 pobj buffer,of
R5418 T18922 T18921 compound homogenization,buffer
R5419 T18923 T18914 punct ", ",diluted
R5420 T18924 T18914 cc and,diluted
R5421 T18925 T18914 conj incubated,diluted
R5422 T18926 T18925 prep with,incubated
R5423 T18927 T18928 nummod 1,μl
R5424 T18928 T18926 pobj μl,with
R5425 T18929 T18928 prep of,μl
R5426 T18930 T18931 compound GFP,antisera
R5427 T18931 T18929 pobj antisera,of
R5428 T18932 T18933 punct (,0092
R5429 T18933 T18931 parataxis 0092,antisera
R5430 T18934 T18933 dep Invitrogen,0092
R5431 T18935 T18933 punct ", ",0092
R5432 T18936 T18933 punct #,0092
R5433 T18937 T18933 nummod 46,0092
R5434 T18938 T18933 punct –,0092
R5435 T18939 T18933 punct ),0092
R5436 T18940 T18931 cc and,antisera
R5437 T18941 T18942 compound protein,sepharose
R5438 T18942 T18931 conj sepharose,antisera
R5439 T18943 T18944 compound A,G
R5440 T18944 T18942 compound G,sepharose
R5441 T18945 T18944 punct /,G
R5442 T18946 T18914 punct .,diluted
R5443 T18948 T18949 prep Following,washed
R5444 T18950 T18951 amod overnight,incubation
R5445 T18951 T18948 pobj incubation,Following
R5446 T18952 T18949 punct ", ",washed
R5447 T18953 T18954 det the,proteins
R5448 T18954 T18949 nsubjpass proteins,washed
R5449 T18955 T18954 amod precipitated,proteins
R5450 T18956 T18949 auxpass were,washed
R5451 T18957 T18949 prep in,washed
R5452 T18958 T18959 compound RIPA,buffer
R5453 T18959 T18957 pobj buffer,in
R5454 T18960 T18949 cc and,washed
R5455 T18961 T18962 advmod finally,boiled
R5456 T18962 T18949 conj boiled,washed
R5457 T18963 T18962 prep in,boiled
R5458 T18964 T18965 nummod 50,μl
R5459 T18965 T18963 pobj μl,in
R5460 T18966 T18965 prep of,μl
R5461 T18967 T18968 compound Laemmli,buffer
R5462 T18968 T18966 pobj buffer,of
R5463 T18969 T18949 punct .,washed
R5464 T18971 T18972 nsubjpass Half,processed
R5465 T18973 T18971 prep of,Half
R5466 T18974 T18975 det the,membrane
R5467 T18975 T18973 pobj membrane,of
R5468 T18976 T18975 amod total,membrane
R5469 T18977 T18975 cc and,membrane
R5470 T18978 T18979 det the,fractions
R5471 T18979 T18975 conj fractions,membrane
R5472 T18980 T18979 compound IP,fractions
R5473 T18981 T18972 auxpass were,processed
R5474 T18982 T18972 prep for,processed
R5475 T18983 T18982 pobj immunoblotting,for
R5476 T18984 T18972 punct .,processed
R5477 T18986 T18987 compound Cell,biotinylation
R5478 T18987 T18989 nsubjpass biotinylation,performed
R5479 T18988 T18987 compound surface,biotinylation
R5480 T18990 T18989 auxpass was,performed
R5481 T18991 T18989 prep in,performed
R5482 T18992 T18993 det a,manner
R5483 T18993 T18991 pobj manner,in
R5484 T18994 T18993 amod similar,manner
R5485 T18995 T18989 punct .,performed
R5486 T18997 T18998 advmod However,transfected
R5487 T18999 T18998 punct ", ",transfected
R5488 T19000 T19001 compound pEGFP,F204V
R5489 T19001 T18998 nsubjpass F204V,transfected
R5490 T19002 T19001 punct -,F204V
R5491 T19003 T19001 compound AQP2,F204V
R5492 T19004 T19001 punct -,F204V
R5493 T19005 T18998 punct ", ",transfected
R5494 T19006 T18998 auxpass was,transfected
R5495 T19007 T18998 prep into,transfected
R5496 T19008 T19009 compound MDCK,cells
R5497 T19009 T19007 pobj cells,into
R5498 T19010 T19011 advmod stably,expressing
R5499 T19011 T19009 acl expressing,cells
R5500 T19012 T19013 amod wild,type
R5501 T19013 T19015 compound type,AQP2
R5502 T19014 T19013 punct -,type
R5503 T19015 T19011 dobj AQP2,expressing
R5504 T19016 T19009 cc and,cells
R5505 T19017 T19009 conj cells,cells
R5506 T19018 T19017 acl made,cells
R5507 T19019 T19018 oprd stable,made
R5508 T19020 T19018 prep with,made
R5509 T19021 T19020 pobj vector,with
R5510 T19022 T19021 advmod alone,vector
R5511 T19023 T18998 punct .,transfected
R5512 T19025 T19026 compound Twenty,four
R5513 T19026 T19028 nummod four,hours
R5514 T19027 T19026 punct -,four
R5515 T19028 T19029 npadvmod hours,post-transfection
R5516 T19029 T19030 advmod post-transfection,stimulated
R5517 T19031 T19030 punct ", ",stimulated
R5518 T19032 T19030 nsubjpass cells,stimulated
R5519 T19033 T19030 auxpass were,stimulated
R5520 T19034 T19030 prep with,stimulated
R5521 T19035 T19034 pobj forskolin,with
R5522 T19036 T19030 punct ", ",stimulated
R5523 T19037 T19030 conj trypsinized,stimulated
R5524 T19038 T19037 punct ", ",trypsinized
R5525 T19039 T19037 conj resuspended,trypsinized
R5526 T19040 T19039 prep in,resuspended
R5527 T19041 T19042 nummod 1,ml
R5528 T19042 T19040 pobj ml,in
R5529 T19043 T19042 prep of,ml
R5530 T19044 T19043 pobj PBS,of
R5531 T19045 T19046 punct (,cells
R5532 T19046 T19042 parataxis cells,ml
R5533 T19047 T19048 quantmod 2.5,106
R5534 T19048 T19046 nummod 106,cells
R5535 T19049 T19048 punct ×,106
R5536 T19050 T19051 punct /,ml
R5537 T19051 T19046 prep ml,cells
R5538 T19052 T19046 punct ),cells
R5539 T19053 T19039 punct ", ",resuspended
R5540 T19054 T19039 cc and,resuspended
R5541 T19055 T19039 conj incubated,resuspended
R5542 T19056 T19055 prep with,incubated
R5543 T19057 T19058 nummod 0.5,mg
R5544 T19058 T19056 pobj mg,with
R5545 T19059 T19058 prep of,mg
R5546 T19060 T19061 compound NHS,biotin
R5547 T19061 T19059 pobj biotin,of
R5548 T19062 T19061 punct -,biotin
R5549 T19063 T19061 compound PEO4,biotin
R5550 T19064 T19061 punct -,biotin
R5551 T19065 T19066 punct (,Biotechnology
R5552 T19066 T19061 parataxis Biotechnology,biotin
R5553 T19067 T19066 compound Pierce,Biotechnology
R5554 T19068 T19066 punct ),Biotechnology
R5555 T19069 T19055 prep for,incubated
R5556 T19070 T19071 nummod 30,min
R5557 T19071 T19069 pobj min,for
R5558 T19072 T19055 prep at,incubated
R5559 T19073 T19074 compound room,temperature
R5560 T19074 T19072 pobj temperature,at
R5561 T19075 T19030 punct .,stimulated
R5562 T19077 T19078 nsubj Cells,washed
R5563 T19079 T19078 aux were,washed
R5564 T19080 T19078 advmod once,washed
R5565 T19081 T19078 prep in,washed
R5566 T19082 T19083 nummod 10,mM
R5567 T19083 T19084 compound mM,Tris
R5568 T19084 T19081 pobj Tris,in
R5569 T19085 T19086 punct (,pH
R5570 T19086 T19084 parataxis pH,Tris
R5571 T19087 T19086 nummod 8,pH
R5572 T19088 T19086 punct ),pH
R5573 T19089 T19078 cc and,washed
R5574 T19090 T19091 nummod three,times
R5575 T19091 T19092 npadvmod times,in
R5576 T19092 T19078 conj in,washed
R5577 T19093 T19092 pobj PBS,in
R5578 T19094 T19078 punct ", ",washed
R5579 T19095 T19096 prep after,purified
R5580 T19096 T19078 advcl purified,washed
R5581 T19097 T19095 pobj which,after
R5582 T19098 T19096 nsubjpass membranes,purified
R5583 T19099 T19096 auxpass were,purified
R5584 T19100 T19096 cc and,purified
R5585 T19101 T19096 conj solubilized,purified
R5586 T19102 T19103 mark as,described
R5587 T19103 T19101 advcl described,solubilized
R5588 T19104 T19103 advmod above,described
R5589 T19105 T19078 punct .,washed
R5590 T19107 T19108 amod Solubilized,membranes
R5591 T19108 T19109 nsubjpass membranes,incubated
R5592 T19110 T19109 auxpass were,incubated
R5593 T19111 T19109 prep with,incubated
R5594 T19112 T19113 nummod 20,μl
R5595 T19113 T19111 pobj μl,with
R5596 T19114 T19113 prep of,μl
R5597 T19115 T19116 amod immobilized,streptavidin
R5598 T19116 T19114 pobj streptavidin,of
R5599 T19117 T19118 punct (,Biotechnology
R5600 T19118 T19116 parataxis Biotechnology,streptavidin
R5601 T19119 T19118 compound Pierce,Biotechnology
R5602 T19120 T19118 punct ),Biotechnology
R5603 T19121 T19109 prep for,incubated
R5604 T19122 T19123 nummod 2,h
R5605 T19123 T19121 pobj h,for
R5606 T19124 T19109 prep at,incubated
R5607 T19125 T19126 nummod 4,°C
R5608 T19126 T19124 pobj °C,at
R5609 T19127 T19109 punct .,incubated
R5610 T19129 T19130 advmod Finally,washed
R5611 T19131 T19132 det the,proteins
R5612 T19132 T19130 nsubjpass proteins,washed
R5613 T19133 T19132 amod precipitated,proteins
R5614 T19134 T19130 auxpass were,washed
R5615 T19135 T19130 prep in,washed
R5616 T19136 T19137 compound RIPA,buffer
R5617 T19137 T19135 pobj buffer,in
R5618 T19138 T19130 cc and,washed
R5619 T19139 T19130 conj boiled,washed
R5620 T19140 T19139 prep in,boiled
R5621 T19141 T19142 nummod 50,μl
R5622 T19142 T19140 pobj μl,in
R5623 T19143 T19142 prep of,μl
R5624 T19144 T19145 compound Laemmli,buffer
R5625 T19145 T19143 pobj buffer,of
R5626 T19146 T19130 punct .,washed
R5627 T19148 T19149 amod Total,cells
R5628 T19149 T19150 nsubj cells,immunoblotting
R5629 T19151 T19149 cc and,cells
R5630 T19152 T19153 det the,precipitates
R5631 T19153 T19149 conj precipitates,cells
R5632 T19154 T19153 amod biotinylated,precipitates
R5633 T19155 T19150 aux were,immunoblotting
R5634 T19156 T19150 advcl using,immunoblotting
R5635 T19157 T19158 det an,antibody
R5636 T19158 T19156 dobj antibody,using
R5637 T19159 T19158 prep to,antibody
R5638 T19160 T19159 pobj AQP2,to
R5639 T19161 T19150 punct .,immunoblotting
R5640 T19603 T19604 compound Kidney,immunohistochemistry
R5641 T19605 T19604 punct .,immunohistochemistry
R5642 T19607 T19608 amod Whole,kidneys
R5643 T19608 T19610 nsubjpass kidneys,fixed
R5644 T19609 T19608 compound mouse,kidneys
R5645 T19611 T19610 auxpass were,fixed
R5646 T19612 T19610 prep in,fixed
R5647 T19613 T19614 nummod 10,%
R5648 T19614 T19615 nmod %,formalin
R5649 T19615 T19612 pobj formalin,in
R5650 T19616 T19617 npadvmod phosphate,buffered
R5651 T19617 T19615 amod buffered,formalin
R5652 T19618 T19617 punct -,buffered
R5653 T19619 T19610 prep for,fixed
R5654 T19620 T19621 nummod 24,h
R5655 T19621 T19619 pobj h,for
R5656 T19622 T19610 punct .,fixed
R5657 T19624 T19625 nsubjpass Kidneys,embedded
R5658 T19626 T19625 auxpass were,embedded
R5659 T19627 T19625 prep in,embedded
R5660 T19628 T19627 pobj paraffin,in
R5661 T19629 T19625 punct ", ",embedded
R5662 T19630 T19625 cc and,embedded
R5663 T19631 T19632 nummod 5,μm
R5664 T19632 T19634 compound μm,sections
R5665 T19633 T19632 punct -,μm
R5666 T19634 T19635 nsubjpass sections,prepared
R5667 T19635 T19625 conj prepared,embedded
R5668 T19636 T19635 auxpass were,prepared
R5669 T19637 T19635 punct .,prepared
R5670 T19639 T19640 prep Following,probed
R5671 T19641 T19642 compound antigen,retrieval
R5672 T19642 T19639 pobj retrieval,Following
R5673 T19643 T19642 acl using,retrieval
R5674 T19644 T19645 nummod 10,mM
R5675 T19645 T19646 compound mM,citrate
R5676 T19646 T19643 dobj citrate,using
R5677 T19647 T19646 compound sodium,citrate
R5678 T19648 T19646 punct (,citrate
R5679 T19649 T19646 npadvmod pH,citrate
R5680 T19650 T19649 nummod 8,pH
R5681 T19651 T19646 punct ),citrate
R5682 T19652 T19643 prep for,using
R5683 T19653 T19654 nummod 10,min
R5684 T19654 T19652 pobj min,for
R5685 T19655 T19643 prep at,using
R5686 T19656 T19657 nummod 98,°C
R5687 T19657 T19655 pobj °C,at
R5688 T19658 T19640 punct ", ",probed
R5689 T19659 T19640 nsubjpass sections,probed
R5690 T19660 T19640 auxpass were,probed
R5691 T19661 T19640 advmod sequentially,probed
R5692 T19662 T19640 punct ", ",probed
R5693 T19663 T19664 advmod first,for
R5694 T19664 T19640 prep for,probed
R5695 T19665 T19664 pobj AQP3,for
R5696 T19666 T19664 cc and,for
R5697 T19667 T19668 advmod then,for
R5698 T19668 T19664 conj for,for
R5699 T19669 T19668 pobj AQP2,for
R5700 T19670 T19640 punct .,probed
R5701 T19672 T19673 nsubjpass Sections,incubated
R5702 T19674 T19673 auxpass were,incubated
R5703 T19675 T19673 prep in,incubated
R5704 T19676 T19677 nummod 5,%
R5705 T19677 T19678 compound %,serum
R5706 T19678 T19675 pobj serum,in
R5707 T19679 T19678 compound donkey,serum
R5708 T19680 T19673 cc and,incubated
R5709 T19681 T19682 advmod then,in
R5710 T19682 T19673 conj in,incubated
R5711 T19683 T19684 nmod goat,antibody
R5712 T19684 T19682 pobj antibody,in
R5713 T19685 T19684 amod anti-AQP3,antibody
R5714 T19686 T19687 punct (,sc
R5715 T19687 T19684 parataxis sc,antibody
R5716 T19688 T19687 dep 1,sc
R5717 T19689 T19690 punct :,100
R5718 T19690 T19688 prep 100,1
R5719 T19691 T19687 punct ;,sc
R5720 T19692 T19693 compound Santa,Cruz
R5721 T19693 T19694 compound Cruz,Biotechnology
R5722 T19694 T19687 dep Biotechnology,sc
R5723 T19695 T19687 punct ;,sc
R5724 T19696 T19687 punct #,sc
R5725 T19697 T19687 punct -,sc
R5726 T19698 T19687 nummod 9885,sc
R5727 T19699 T19687 punct ),sc
R5728 T19700 T19673 punct .,incubated
R5729 T19702 T19703 nsubjpass Slides,washed
R5730 T19704 T19703 auxpass were,washed
R5731 T19705 T19703 prep with,washed
R5732 T19706 T19705 pobj PBS,with
R5733 T19707 T19703 cc and,washed
R5734 T19708 T19703 conj incubated,washed
R5735 T19709 T19708 prep with,incubated
R5736 T19710 T19711 npadvmod AlexaFluor,conjugated
R5737 T19711 T19714 amod conjugated,antibody
R5738 T19712 T19710 nummod 488,AlexaFluor
R5739 T19713 T19711 punct -,conjugated
R5740 T19714 T19709 pobj antibody,with
R5741 T19715 T19714 nmod donkey,antibody
R5742 T19716 T19714 amod anti-goat,antibody
R5743 T19717 T19718 punct (,Probes
R5744 T19718 T19714 parataxis Probes,antibody
R5745 T19719 T19718 compound Molecular,Probes
R5746 T19720 T19718 punct ", ",Probes
R5747 T19721 T19718 npadvmod Eugene,Probes
R5748 T19722 T19718 punct ", ",Probes
R5749 T19723 T19718 npadvmod Oregon,Probes
R5750 T19724 T19718 punct ", ",Probes
R5751 T19725 T19726 compound United,States
R5752 T19726 T19718 npadvmod States,Probes
R5753 T19727 T19718 punct ),Probes
R5754 T19728 T19703 punct .,washed
R5755 T19730 T19731 det The,slides
R5756 T19731 T19732 nsubjpass slides,incubated
R5757 T19733 T19732 auxpass were,incubated
R5758 T19734 T19732 advmod subsequently,incubated
R5759 T19735 T19732 aux blocked,incubated
R5760 T19736 T19732 prep in,incubated
R5761 T19737 T19738 nummod 5,%
R5762 T19738 T19739 compound %,serum
R5763 T19739 T19736 pobj serum,in
R5764 T19740 T19739 compound chicken,serum
R5765 T19741 T19732 punct ", ",incubated
R5766 T19742 T19732 prep with,incubated
R5767 T19743 T19744 det a,antibody
R5768 T19744 T19742 pobj antibody,with
R5769 T19745 T19744 nmod rabbit,antibody
R5770 T19746 T19744 amod anti-AQP2,antibody
R5771 T19747 T19748 punct (,A3000
R5772 T19748 T19744 parataxis A3000,antibody
R5773 T19749 T19748 dep 1,A3000
R5774 T19750 T19751 punct :,250
R5775 T19751 T19749 prep 250,1
R5776 T19752 T19748 punct ;,A3000
R5777 T19753 T19748 dep USB,A3000
R5778 T19754 T19753 punct ", ",USB
R5779 T19755 T19753 npadvmod Cleveland,USB
R5780 T19756 T19753 punct ", ",USB
R5781 T19757 T19753 npadvmod Ohio,USB
R5782 T19758 T19753 punct ", ",USB
R5783 T19759 T19760 compound United,States
R5784 T19760 T19753 npadvmod States,USB
R5785 T19761 T19748 punct ;,A3000
R5786 T19762 T19748 punct #,A3000
R5787 T19763 T19748 punct –,A3000
R5788 T19764 T19748 nummod 06,A3000
R5789 T19765 T19748 punct ),A3000
R5790 T19766 T19744 punct ", ",antibody
R5791 T19767 T19768 dep which,detected
R5792 T19768 T19744 relcl detected,antibody
R5793 T19769 T19768 auxpass was,detected
R5794 T19770 T19768 prep with,detected
R5795 T19771 T19772 det a,antibody
R5796 T19772 T19770 pobj antibody,with
R5797 T19773 T19774 npadvmod AlexaFluor,conjugated
R5798 T19774 T19772 amod conjugated,antibody
R5799 T19775 T19773 nummod 594,AlexaFluor
R5800 T19776 T19774 punct -,conjugated
R5801 T19777 T19772 nmod chicken,antibody
R5802 T19778 T19772 amod anti-rabbit,antibody
R5803 T19779 T19780 punct (,Probes
R5804 T19780 T19772 parataxis Probes,antibody
R5805 T19781 T19780 dep 1,Probes
R5806 T19782 T19783 punct :,500
R5807 T19783 T19781 prep 500,1
R5808 T19784 T19780 punct ;,Probes
R5809 T19785 T19780 compound Molecular,Probes
R5810 T19786 T19780 punct ),Probes
R5811 T19787 T19732 punct .,incubated
R5812 T19789 T19790 det The,sections
R5813 T19790 T19791 nsubjpass sections,stained
R5814 T19792 T19791 auxpass were,stained
R5815 T19793 T19791 prep with,stained
R5816 T19794 T19793 pobj DAPI,with
R5817 T19795 T19791 cc and,stained
R5818 T19796 T19791 conj mounted,stained
R5819 T19797 T19796 prep in,mounted
R5820 T19798 T19797 pobj Vectashield,in
R5821 T19799 T19800 punct (,Labs
R5822 T19800 T19796 parataxis Labs,mounted
R5823 T19801 T19800 compound Vector,Labs
R5824 T19802 T19800 punct ", ",Labs
R5825 T19803 T19800 npadvmod Burlingame,Labs
R5826 T19804 T19800 punct ", ",Labs
R5827 T19805 T19800 npadvmod California,Labs
R5828 T19806 T19800 punct ", ",Labs
R5829 T19807 T19808 compound United,States
R5830 T19808 T19800 npadvmod States,Labs
R5831 T19809 T19800 punct ),Labs
R5832 T19810 T19791 punct .,stained
R5833 T20425 T20424 prep on,Immunocytochemistry
R5834 T20426 T20427 compound MDCK,cells
R5835 T20427 T20425 pobj cells,on
R5836 T20428 T20424 punct .,Immunocytochemistry
R5837 T20430 T20431 nmod MDCK,lines
R5838 T20431 T20434 nsubjpass lines,grown
R5839 T20432 T20431 amod stable,lines
R5840 T20433 T20431 compound cell,lines
R5841 T20435 T20431 acl expressing,lines
R5842 T20436 T20435 dobj vector,expressing
R5843 T20437 T20436 amod alone,vector
R5844 T20438 T20436 punct ", ",vector
R5845 T20439 T20440 amod wild,type
R5846 T20440 T20442 compound type,AQP2
R5847 T20441 T20440 punct -,type
R5848 T20442 T20436 conj AQP2,vector
R5849 T20443 T20442 punct ", ",AQP2
R5850 T20444 T20442 cc or,AQP2
R5851 T20445 T20446 compound AQP2,F204V
R5852 T20446 T20442 conj F204V,AQP2
R5853 T20447 T20446 punct -,F204V
R5854 T20448 T20435 punct (,expressing
R5855 T20449 T20435 cc and,expressing
R5856 T20450 T20451 prep in,expressing
R5857 T20451 T20435 conj expressing,expressing
R5858 T20452 T20453 det some,cases
R5859 T20453 T20450 pobj cases,in
R5860 T20454 T20451 advmod transiently,expressing
R5861 T20455 T20456 det a,construct
R5862 T20456 T20451 dobj construct,expressing
R5863 T20457 T20456 compound GFP,construct
R5864 T20458 T20451 punct ),expressing
R5865 T20459 T20434 auxpass were,grown
R5866 T20460 T20434 prep on,grown
R5867 T20461 T20462 npadvmod fibronectin,coated
R5868 T20462 T20464 amod coated,coverslips
R5869 T20463 T20462 punct -,coated
R5870 T20464 T20460 pobj coverslips,on
R5871 T20465 T20466 mark until,formed
R5872 T20466 T20434 advcl formed,grown
R5873 T20467 T20468 amod tight,junctions
R5874 T20468 T20466 nsubj junctions,formed
R5875 T20469 T20434 punct .,grown
R5876 T20471 T20472 nsubjpass Cells,treated
R5877 T20473 T20472 auxpass were,treated
R5878 T20474 T20472 prep with,treated
R5879 T20475 T20474 cc or,with
R5880 T20476 T20474 conj without,with
R5881 T20477 T20478 nummod 150,μM
R5882 T20478 T20479 compound μM,forskolin
R5883 T20479 T20476 pobj forskolin,without
R5884 T20480 T20472 prep for,treated
R5885 T20481 T20482 nummod 90,min
R5886 T20482 T20480 pobj min,for
R5887 T20483 T20472 punct ", ",treated
R5888 T20484 T20472 cc and,treated
R5889 T20485 T20472 conj fixed,treated
R5890 T20486 T20485 prep in,fixed
R5891 T20487 T20486 pobj methanol,in
R5892 T20488 T20485 prep at,fixed
R5893 T20489 T20490 punct −,20
R5894 T20490 T20491 nummod 20,°C
R5895 T20491 T20488 pobj °C,at
R5896 T20492 T20472 punct .,treated
R5897 T20494 T20495 advmod Subsequently,washed
R5898 T20496 T20495 punct ", ",washed
R5899 T20497 T20495 nsubj cells,washed
R5900 T20498 T20495 aux were,washed
R5901 T20499 T20495 cc and,washed
R5902 T20500 T20495 conj permeabilized,washed
R5903 T20501 T20500 prep in,permeabilized
R5904 T20502 T20503 nummod 0.2,%
R5905 T20503 T20504 compound %,X
R5906 T20504 T20501 pobj X,in
R5907 T20505 T20504 compound Triton,X
R5908 T20506 T20504 punct -,X
R5909 T20507 T20504 nummod 100,X
R5910 T20508 T20500 prep for,permeabilized
R5911 T20509 T20510 nummod 5,min
R5912 T20510 T20508 pobj min,for
R5913 T20511 T20495 punct ", ",washed
R5914 T20512 T20495 cc and,washed
R5915 T20513 T20514 advmod sequentially,probed
R5916 T20514 T20495 conj probed,washed
R5917 T20515 T20514 prep for,probed
R5918 T20516 T20515 pobj AQP2,for
R5919 T20517 T20516 cc and,AQP2
R5920 T20518 T20519 compound organelle,markers
R5921 T20519 T20516 conj markers,AQP2
R5922 T20520 T20519 prep for,markers
R5923 T20521 T20522 preconj either,PM
R5924 T20522 T20520 pobj PM,for
R5925 T20523 T20522 det the,PM
R5926 T20524 T20522 cc or,PM
R5927 T20525 T20526 det the,ER
R5928 T20526 T20522 conj ER,PM
R5929 T20527 T20495 punct .,washed
R5930 T20529 T20530 nsubjpass AQP2,detected
R5931 T20531 T20530 auxpass was,detected
R5932 T20532 T20530 advcl using,detected
R5933 T20533 T20532 dobj goat,using
R5934 T20534 T20533 amod anti-AQP2,goat
R5935 T20535 T20536 punct (,sc
R5936 T20536 T20533 parataxis sc,goat
R5937 T20537 T20536 dep 1,sc
R5938 T20538 T20539 punct :,100
R5939 T20539 T20537 prep 100,1
R5940 T20540 T20536 punct ;,sc
R5941 T20541 T20542 compound Santa,Cruz
R5942 T20542 T20543 compound Cruz,Biotechnology
R5943 T20543 T20536 dep Biotechnology,sc
R5944 T20544 T20536 punct ;,sc
R5945 T20545 T20536 punct #,sc
R5946 T20546 T20536 punct -,sc
R5947 T20547 T20536 nummod 9882,sc
R5948 T20548 T20536 punct ),sc
R5949 T20549 T20533 cc and,goat
R5950 T20550 T20551 det a,dilution
R5951 T20551 T20533 conj dilution,goat
R5952 T20552 T20553 quantmod 1,200
R5953 T20553 T20551 nummod 200,dilution
R5954 T20554 T20553 punct :,200
R5955 T20555 T20551 prep of,dilution
R5956 T20556 T20557 npadvmod AlexaFluor,conjugated
R5957 T20557 T20560 amod conjugated,antibody
R5958 T20558 T20556 nummod 488,AlexaFluor
R5959 T20559 T20557 punct -,conjugated
R5960 T20560 T20555 pobj antibody,of
R5961 T20561 T20560 nmod donkey,antibody
R5962 T20562 T20560 amod anti-goat,antibody
R5963 T20563 T20560 amod secondary,antibody
R5964 T20564 T20530 punct .,detected
R5965 T20566 T20567 det The,PM
R5966 T20567 T20568 nsubjpass PM,probed
R5967 T20569 T20567 cc and,PM
R5968 T20570 T20567 conj ER,PM
R5969 T20571 T20568 auxpass were,probed
R5970 T20572 T20568 advcl using,probed
R5971 T20573 T20574 nmod mouse,ATPase
R5972 T20574 T20579 nmod ATPase,antibodies
R5973 T20575 T20574 amod anti-Na+,ATPase
R5974 T20576 T20574 punct /,ATPase
R5975 T20577 T20574 nmod K+,ATPase
R5976 T20578 T20574 punct -,ATPase
R5977 T20579 T20572 dobj antibodies,using
R5978 T20580 T20581 punct (,Upstate
R5979 T20581 T20574 parataxis Upstate,ATPase
R5980 T20582 T20581 punct ", ",Upstate
R5981 T20583 T20581 npadvmod Waltham,Upstate
R5982 T20584 T20581 punct ", ",Upstate
R5983 T20585 T20581 npadvmod Massachusetts,Upstate
R5984 T20586 T20581 punct ", ",Upstate
R5985 T20587 T20588 compound United,States
R5986 T20588 T20581 npadvmod States,Upstate
R5987 T20589 T20581 punct ),Upstate
R5988 T20590 T20574 cc or,ATPase
R5989 T20591 T20592 npadvmod rabbit,anti-calnexin
R5990 T20592 T20574 conj anti-calnexin,ATPase
R5991 T20593 T20594 punct (,Biotechnology
R5992 T20594 T20592 parataxis Biotechnology,anti-calnexin
R5993 T20595 T20594 compound Stressgen,Biotechnology
R5994 T20596 T20594 punct ", ",Biotechnology
R5995 T20597 T20594 npadvmod Victoria,Biotechnology
R5996 T20598 T20594 punct ", ",Biotechnology
R5997 T20599 T20600 compound British,Columbia
R5998 T20600 T20594 npadvmod Columbia,Biotechnology
R5999 T20601 T20594 punct ", ",Biotechnology
R6000 T20602 T20594 npadvmod Canada,Biotechnology
R6001 T20603 T20594 punct ),Biotechnology
R6002 T20604 T20579 cc and,antibodies
R6003 T20605 T20606 det the,antibodies
R6004 T20606 T20579 conj antibodies,antibodies
R6005 T20607 T20606 amod secondary,antibodies
R6006 T20608 T20606 punct ", ",antibodies
R6007 T20609 T20610 npadvmod Cy3,conjugated
R6008 T20610 T20612 amod conjugated,goat
R6009 T20611 T20610 punct -,conjugated
R6010 T20612 T20606 appos goat,antibodies
R6011 T20613 T20612 amod anti-mouse,goat
R6012 T20614 T20615 punct (,ImmunoResearch
R6013 T20615 T20612 parataxis ImmunoResearch,goat
R6014 T20616 T20615 dep 1,ImmunoResearch
R6015 T20617 T20618 punct :,200
R6016 T20618 T20616 prep 200,1
R6017 T20619 T20615 punct ;,ImmunoResearch
R6018 T20620 T20615 compound Jackson,ImmunoResearch
R6019 T20621 T20615 punct ", ",ImmunoResearch
R6020 T20622 T20623 compound West,Grove
R6021 T20623 T20615 npadvmod Grove,ImmunoResearch
R6022 T20624 T20615 punct ", ",ImmunoResearch
R6023 T20625 T20615 npadvmod Pennsylvania,ImmunoResearch
R6024 T20626 T20615 punct ", ",ImmunoResearch
R6025 T20627 T20628 compound United,States
R6026 T20628 T20615 npadvmod States,ImmunoResearch
R6027 T20629 T20615 punct ),ImmunoResearch
R6028 T20630 T20606 cc or,antibodies
R6029 T20631 T20632 npadvmod AlexaFluor,conjugated
R6030 T20632 T20635 amod conjugated,chicken
R6031 T20633 T20631 nummod 594,AlexaFluor
R6032 T20634 T20632 punct -,conjugated
R6033 T20635 T20606 conj chicken,antibodies
R6034 T20636 T20635 amod anti-rabbit,chicken
R6035 T20637 T20638 punct (,1
R6036 T20638 T20635 parataxis 1,chicken
R6037 T20639 T20640 punct :,200
R6038 T20640 T20638 prep 200,1
R6039 T20641 T20638 punct ),1
R6040 T20642 T20572 advmod respectively,using
R6041 T20643 T20568 punct .,probed
R6042 T20645 T20646 nsubjpass Cells,washed
R6043 T20647 T20646 auxpass were,washed
R6044 T20648 T20646 prep in,washed
R6045 T20649 T20648 pobj PBS,in
R6046 T20650 T20646 punct ", ",washed
R6047 T20651 T20646 conj counterstained,washed
R6048 T20652 T20651 prep with,counterstained
R6049 T20653 T20652 pobj DAPI,with
R6050 T20654 T20651 punct ", ",counterstained
R6051 T20655 T20651 cc and,counterstained
R6052 T20656 T20651 conj mounted,counterstained
R6053 T20657 T20656 prep in,mounted
R6054 T20658 T20657 pobj Vectashield,in
R6055 T20659 T20646 punct .,washed
R6056 T20661 T20662 prep In,probed
R6057 T20663 T20661 pobj experiments,In
R6058 T20664 T20665 prep in,used
R6059 T20665 T20663 relcl used,experiments
R6060 T20666 T20664 pobj which,in
R6061 T20667 T20668 compound GFP,fusions
R6062 T20668 T20665 nsubjpass fusions,used
R6063 T20669 T20665 auxpass were,used
R6064 T20670 T20662 punct ", ",probed
R6065 T20671 T20662 nsubjpass AQP2,probed
R6066 T20672 T20662 auxpass was,probed
R6067 T20673 T20662 advcl using,probed
R6068 T20674 T20675 det the,combination
R6069 T20675 T20673 dobj combination,using
R6070 T20676 T20675 compound antibody,combination
R6071 T20677 T20675 acl used,combination
R6072 T20678 T20677 prep for,used
R6073 T20679 T20680 compound kidney,immunohistochemistry
R6074 T20680 T20678 pobj immunohistochemistry,for
R6075 T20681 T20662 prep in,probed
R6076 T20682 T20681 pobj order,in
R6077 T20683 T20684 aux to,detect
R6078 T20684 T20682 acl detect,order
R6079 T20685 T20686 det the,AQP2
R6080 T20686 T20684 dobj AQP2,detect
R6081 T20687 T20684 prep at,detect
R6082 T20688 T20689 nummod 594,nm
R6083 T20689 T20687 pobj nm,at
R6084 T20690 T20684 punct ", ",detect
R6085 T20691 T20692 aux to,distinguish
R6086 T20692 T20684 advcl distinguish,detect
R6087 T20693 T20692 prep between,distinguish
R6088 T20694 T20695 det the,proteins
R6089 T20695 T20693 pobj proteins,between
R6090 T20696 T20695 compound GFP,proteins
R6091 T20697 T20695 compound fusion,proteins
R6092 T20698 T20662 punct .,probed
R6093 T20832 T20833 amod Confocal,microscopy
R6094 T20834 T20833 punct .,microscopy
R6095 T20836 T20837 amod Optical,images
R6096 T20837 T20841 nsubjpass images,collected
R6097 T20838 T20839 compound z,section
R6098 T20839 T20837 compound section,images
R6099 T20840 T20839 punct -,section
R6100 T20842 T20841 auxpass were,collected
R6101 T20843 T20841 prep on,collected
R6102 T20844 T20845 det a,Microscope
R6103 T20845 T20843 pobj Microscope,on
R6104 T20846 T20845 nmod BioRad,Microscope
R6105 T20847 T20848 punct (,Hercules
R6106 T20848 T20846 parataxis Hercules,BioRad
R6107 T20849 T20848 punct ", ",Hercules
R6108 T20850 T20848 npadvmod California,Hercules
R6109 T20851 T20848 punct ", ",Hercules
R6110 T20852 T20853 compound United,States
R6111 T20853 T20848 npadvmod States,Hercules
R6112 T20854 T20848 punct ),Hercules
R6113 T20855 T20856 nmod Rainbow,Radiance
R6114 T20856 T20845 nmod Radiance,Microscope
R6115 T20857 T20856 nummod 2100,Radiance
R6116 T20858 T20859 compound Laser,Scanning
R6117 T20859 T20845 compound Scanning,Microscope
R6118 T20860 T20845 compound Confocal,Microscope
R6119 T20861 T20841 punct .,collected
R6120 T20863 T20864 compound Image,stacks
R6121 T20864 T20865 nsubjpass stacks,flattened
R6122 T20866 T20865 auxpass were,flattened
R6123 T20867 T20865 punct ", ",flattened
R6124 T20868 T20865 cc or,flattened
R6125 T20869 T20865 conj sectioned,flattened
R6126 T20870 T20869 prep along,sectioned
R6127 T20871 T20872 det the,axis
R6128 T20872 T20870 pobj axis,along
R6129 T20873 T20872 compound z,axis
R6130 T20874 T20872 punct -,axis
R6131 T20875 T20865 punct ", ",flattened
R6132 T20876 T20877 advmod then,processed
R6133 T20877 T20865 dep processed,flattened
R6134 T20878 T20877 advmod further,processed
R6135 T20879 T20877 advcl using,processed
R6136 T20880 T20881 nmod BioRad,software
R6137 T20881 T20879 dobj software,using
R6138 T20882 T20883 nmod Laser,Sharp
R6139 T20883 T20881 nmod Sharp,software
R6140 T20884 T20883 nummod 2000,Sharp
R6141 T20885 T20881 cc and,software
R6142 T20886 T20887 compound Image,J
R6143 T20887 T20888 compound J,software
R6144 T20888 T20881 conj software,software
R6145 T20889 T20890 punct (,Institutes
R6146 T20890 T20888 parataxis Institutes,software
R6147 T20891 T20890 dep v.,Institutes
R6148 T20892 T20891 nummod 1.32,v.
R6149 T20893 T20890 punct ;,Institutes
R6150 T20894 T20890 compound National,Institutes
R6151 T20895 T20890 prep of,Institutes
R6152 T20896 T20895 pobj Health,of
R6153 T20897 T20890 punct ),Institutes
R6154 T20898 T20865 punct .,flattened
R6155 T20900 T20901 nsubjpass Colocalization,performed
R6156 T20902 T20901 auxpass was,performed
R6157 T20903 T20901 advcl using,performed
R6158 T20904 T20905 det the,coefficient
R6159 T20905 T20903 dobj coefficient,using
R6160 T20906 T20905 amod overlay,coefficient
R6161 T20907 T20905 prep of,coefficient
R6162 T20908 T20909 compound Image,J
R6163 T20909 T20910 compound J,software
R6164 T20910 T20907 pobj software,of
R6165 T20911 T20901 punct .,performed
R45 T645 T643 prep in,harbor
R46 T646 T647 det the,gene
R54 T654 T647 cc or,gene
R55 T655 T656 det the,gene
R58 T658 T656 amod sensitive,gene
R1 T593 T594 compound Diabetes,Insipidus
R2 T596 T594 prep in,Insipidus
R3 T597 T596 pobj Mice,in
R4 T598 T597 prep with,Mice
R5 T599 T600 det a,Mutation
R6 T600 T598 pobj Mutation,with
R7 T601 T600 prep in,Mutation
R8 T602 T601 pobj Aquaporin,in
R9 T603 T602 punct -,Aquaporin
R10 T604 T602 nummod 2,Aquaporin
R11 T608 T609 amod Congenital,insipidus
R15 T613 T609 punct (,insipidus
R16 T614 T609 appos NDI,insipidus
R17 T615 T609 punct ),insipidus
R18 T616 T617 det a,disease
R19 T617 T612 attr disease,is
R20 T618 T617 acl characterized,disease
R21 T619 T618 agent by,characterized
R22 T620 T619 pobj failure,by
R23 T621 T620 prep of,failure
R24 T622 T623 det the,kidney
R25 T623 T621 pobj kidney,of
R26 T624 T625 aux to,concentrate
R27 T625 T620 acl concentrate,failure
R28 T626 T625 dobj urine,concentrate
R29 T627 T625 prep in,concentrate
R30 T628 T627 pobj response,in
R31 T629 T628 prep to,response
R32 T630 T629 pobj vasopressin,to
R33 T631 T612 punct .,is
R34 T633 T634 amod Human,kindreds
R35 T634 T635 nsubjpass kindreds,found
R36 T636 T634 prep with,kindreds
R37 T637 T638 amod nephrogenic,insipidus
R40 T640 T635 aux have,found
R41 T641 T635 auxpass been,found
R42 T642 T643 aux to,harbor
R43 T643 T635 xcomp harbor,found
R44 T644 T643 dobj mutations,harbor
R68 T668 T635 punct .,found
R69 T670 T671 nsubjpass Development,rendered
R70 T672 T670 prep of,Development
R71 T673 T674 det a,treatment
R72 T674 T672 pobj treatment,of
R73 T675 T671 auxpass is,rendered
R74 T676 T671 oprd difficult,rendered
R75 T677 T671 prep due,rendered
R76 T678 T677 pcomp to,due
R77 T679 T680 det the,lack
R78 T680 T677 pobj lack,due
R79 T681 T680 prep of,lack
R80 T682 T683 det a,model
R84 T686 T671 punct .,rendered
R85 T688 T689 prep Through,report
R86 T690 T691 amod forward,screening
R89 T693 T691 prep of,screening
R90 T694 T695 npadvmod ethylnitrosourea,mutagenized
R93 T697 T693 pobj mice,of
R94 T698 T689 punct ", ",report
R95 T699 T689 nsubj we,report
R96 T700 T701 det the,identification
R97 T701 T689 dobj identification,report
R98 T702 T701 cc and,identification
R99 T703 T701 conj characterization,identification
R100 T704 T701 prep of,identification
R101 T705 T706 det a,model
R104 T708 T706 prep of,model
R105 T709 T708 pobj NDI,of
R106 T710 T706 punct ", ",model
R107 T711 T706 prep with,model
R108 T712 T713 det an,mutation
R111 T715 T713 prep in,mutation
R112 T716 T717 det the,gene
R115 T719 T689 punct .,report
R116 T721 T722 prep Unlike,survive
R117 T723 T724 advmod previously,attempted
R118 T724 T725 amod attempted,models
R121 T727 T725 prep of,models
R122 T728 T727 pobj NDI,of
R123 T729 T722 punct ", ",survive
R124 T730 T731 poss our,mice
R125 T731 T722 nsubj mice,survive
R126 T732 T722 prep to,survive
R127 T733 T732 pobj adulthood,to
R128 T734 T722 cc and,survive
R129 T735 T736 advmod more,exactly
R130 T736 T737 advmod exactly,recapitulate
R131 T737 T722 conj recapitulate,survive
R132 T738 T739 det the,disorder
R135 T741 T722 punct .,survive
R136 T743 T744 amod Previous,experiments
R140 T748 T744 acl using,experiments
R141 T749 T750 amod renal,lines
R144 T752 T753 amod recessive,mutations
R147 T755 T747 advcl result,suggest
R148 T756 T755 prep in,result
R149 T757 T758 amod improper,trafficking
R150 T758 T756 pobj trafficking,in
R151 T759 T758 prep of,trafficking
R152 T760 T761 det the,pore
R156 T764 T747 punct .,suggest
R157 T766 T767 advcl Using,proven
R158 T768 T769 det these,animals
R159 T769 T766 dobj animals,Using
R160 T770 T767 punct ", ",proven
R161 T771 T767 nsubj we,proven
R162 T772 T767 aux have,proven
R163 T773 T767 advmod directly,proven
R164 T774 T775 det this,hypothesis
R165 T775 T767 dobj hypothesis,proven
R166 T776 T775 prep of,hypothesis
R167 T777 T778 amod improper,translocation
R170 T780 T778 prep as,translocation
R171 T781 T782 det the,defect
R174 T784 T782 prep in,defect
R175 T785 T786 amod nephrogenic,insipidus
R178 T788 T782 prep in,defect
R179 T789 T790 det the,organism
R182 T792 T767 punct .,proven
R183 T794 T795 advmod Additionally,show
R184 T796 T795 punct ", ",show
R185 T797 T795 advcl using,show
R186 T798 T799 det a,line
R190 T802 T795 nsubj we,show
R191 T803 T804 mark that,retained
R194 T806 T804 nsubjpass protein,retained
R198 T810 T806 appos F204V,protein
R202 T814 T804 prep in,retained
R203 T815 T816 det the,reticulum
R206 T818 T804 cc and,retained
R207 T819 T820 mark that,rescued
R210 T822 T820 nsubjpass localization,rescued
R214 T826 T820 agent by,rescued
R215 T827 T828 amod wild,type
R218 T830 T826 pobj protein,by
R219 T831 T795 punct .,show
R220 T833 T834 det This,model
R224 T838 T837 prep for,allows
R225 T839 T840 amod further,studies
R228 T842 T843 advmod as,as
R231 T845 T840 conj testing,studies
R232 T846 T845 prep of,testing
R233 T847 T848 amod pharmacological,therapies
R237 T851 T845 prep for,testing
R238 T852 T851 pobj NDI,for
R239 T853 T837 punct .,allows

2_test

Id Subject Object Predicate Lexical cue
16121255-1303271-85587770 3364-3365 1303271 denotes 2
16121255-8140421-85587771 3415-3416 8140421 denotes 3
16121255-15340377-85587772 3505-3506 15340377 denotes 4
16121255-8429910-85587773 3579-3580 8429910 denotes 5
16121255-15340377-85587774 3928-3929 15340377 denotes 4
16121255-14662748-85587775 3930-3931 14662748 denotes 6
16121255-7573395-85587776 4499-4500 7573395 denotes 7
16121255-7532304-85587777 4501-4502 7532304 denotes 8
16121255-9169447-85587778 4754-4755 9169447 denotes 9
16121255-8140421-85587779 4947-4948 8140421 denotes 3
16121255-7524315-85587780 4949-4951 7524315 denotes 10
16121255-14662748-85587781 5130-5131 14662748 denotes 6
16121255-7537761-85587782 5132-5134 7537761 denotes 11
16121255-14662748-85587783 5282-5283 14662748 denotes 6
16121255-12787389-85587784 5284-5286 12787389 denotes 12
16121255-10737773-85587785 5353-5355 10737773 denotes 13
16121255-9468475-85587785 5353-5355 9468475 denotes 13
16121255-11104789-85587785 5353-5355 11104789 denotes 13
16121255-11035042-85587785 5353-5355 11035042 denotes 13
16121255-9916798-85587785 5353-5355 9916798 denotes 13
16121255-11104789-85587786 5461-5463 11104789 denotes 15
16121255-11035038-85587787 5464-5466 11035038 denotes 18
16121255-15064401-85587788 6877-6879 15064401 denotes 19
16121255-11567631-85587789 6880-6882 11567631 denotes 20
16121255-11793119-85587790 9950-9952 11793119 denotes 21
16121255-11035038-85587791 10705-10707 11035038 denotes 18
16121255-9916798-85587792 12542-12544 9916798 denotes 17
16121255-11035038-85587793 13184-13186 11035038 denotes 18
16121255-14593099-85587794 13187-13189 14593099 denotes 22
16121255-14593099-85587795 13623-13625 14593099 denotes 22
16121255-7517548-85587796 16054-16056 7517548 denotes 23
16121255-11929850-85587797 20155-20157 11929850 denotes 24
16121255-14593099-85587798 20204-20206 14593099 denotes 22
16121255-14593099-85587799 20976-20978 14593099 denotes 22
16121255-15509592-85587800 21141-21143 15509592 denotes 25
16121255-11104789-85587801 26139-26141 11104789 denotes 15
16121255-11035038-85587802 26559-26561 11035038 denotes 18
16121255-11035038-85587803 26939-26941 11035038 denotes 18
16121255-12470740-85587804 29140-29142 12470740 denotes 26
16121255-14662748-85587805 30540-30541 14662748 denotes 6
16121255-15509592-85587806 31221-31223 15509592 denotes 25
16121255-9593782-85587807 31227-31229 9593782 denotes 27
16121255-15064401-85587808 31835-31837 15064401 denotes 19
T75474 3364-3365 1303271 denotes 2
T23969 3415-3416 8140421 denotes 3
T80160 3505-3506 15340377 denotes 4
T58437 3579-3580 8429910 denotes 5
T25086 3928-3929 15340377 denotes 4
T18302 3930-3931 14662748 denotes 6
T9860 4499-4500 7573395 denotes 7
T15323 4501-4502 7532304 denotes 8
T75916 4754-4755 9169447 denotes 9
T18141 4947-4948 8140421 denotes 3
T51778 4949-4951 7524315 denotes 10
T58588 5130-5131 14662748 denotes 6
T19533 5132-5134 7537761 denotes 11
T63695 5282-5283 14662748 denotes 6
T49393 5284-5286 12787389 denotes 12
T73785 5353-5355 10737773 denotes 13
T69562 5353-5355 9468475 denotes 13
T43226 5353-5355 11104789 denotes 13
T51402 5353-5355 11035042 denotes 13
T85688 5353-5355 9916798 denotes 13
T21889 5461-5463 11104789 denotes 15
T948 5464-5466 11035038 denotes 18
T47462 6877-6879 15064401 denotes 19
T2172 6880-6882 11567631 denotes 20
T493 9950-9952 11793119 denotes 21
T51608 10705-10707 11035038 denotes 18
T17076 12542-12544 9916798 denotes 17
T44574 13184-13186 11035038 denotes 18
T91505 13187-13189 14593099 denotes 22
T46479 13623-13625 14593099 denotes 22
T63600 16054-16056 7517548 denotes 23
T18045 20155-20157 11929850 denotes 24
T35488 20204-20206 14593099 denotes 22
T95687 20976-20978 14593099 denotes 22
T49378 21141-21143 15509592 denotes 25
T41480 26139-26141 11104789 denotes 15
T81534 26559-26561 11035038 denotes 18
T96150 26939-26941 11035038 denotes 18
T95394 29140-29142 12470740 denotes 26
T49784 30540-30541 14662748 denotes 6
T81260 31221-31223 15509592 denotes 25
T11716 31227-31229 9593782 denotes 27
T38192 31835-31837 15064401 denotes 19