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retinas were then rinsed four times for 15 min each with Sorenson's buffer (pH 7.4). They were then stained for 2 h at 4 °C with 1% osmium tetroxide in 1.5% potassium ferrocyanide. Next, the retinas were rinsed four times for 15 min in maleate buffer (pH 5.1) and then stained for 2 h at room temperature with 1% uranyl acetate in maleate buffer (pH 6.2). They were then washed four times for 15 min with maleate buffer (pH 5.1); once for 10 min with 70% ethanol; once for 10 min with 95% ethanol; and four times for 30 min with 100% ethanol. Next, the retinas were washed three times over one hour with propylene oxide and then embedded in TAAB 812 Resin (Marivac, Quebec, Canada) for 1–2 d in a 60 °C oven. Semi-thin sections were cut at a thickness of 0.5 μm and stained with 1% toluidine blue in 1% sodium borate buffer. Images of semi-thin sections from the mutant retina were taken within the ventral two-thirds of the retina where the majority of the supernumerary S-opsin–expressing cells reside. Wild-type images were taken in comparable regions. Sections for electron microscopy were cut at a thickness of 95 nm, placed on grids, and poststained with 2% uranyl acetate followed by 0.2% lead citrate. All sections were cut in a plane perpendicular to the plane of the photoreceptor layer. They were then visualized on a Jeol 1200EX electron microscope (Jeol, Tokyo, Japan). The electron microscopic images in Figure 7 derive from the ventral two-thirds of the wild-type and mutant retinas."}

{"project":"craft-ca-core-ex-dev","denotations":[{"id":"T13448","span":{"begin":4,"end":11},"obj":"UBERON:0000966"},{"id":"T13449","span":{"begin":72,"end":78},"obj":"CHEBI_CHMO_EXT:buffer_solution"},{"id":"T13450","span":{"begin":161,"end":183},"obj":"CHEBI:30059"},{"id":"T13451","span":{"begin":195,"end":202},"obj":"UBERON:0000966"},{"id":"T13452","span":{"begin":240,"end":247},"obj":"CHEBI_EXT:maleate"},{"id":"T13453","span":{"begin":248,"end":254},"obj":"CHEBI_CHMO_EXT:buffer_solution"},{"id":"T13454","span":{"begin":335,"end":342},"obj":"CHEBI_EXT:maleate"},{"id":"T13455","span":{"begin":343,"end":349},"obj":"CHEBI_CHMO_EXT:buffer_solution"},{"id":"T13456","span":{"begin":409,"end":416},"obj":"CHEBI_EXT:maleate"},{"id":"T13457","span":{"begin":417,"end":423},"obj":"CHEBI_CHMO_EXT:buffer_solution"},{"id":"T13458","span":{"begin":459,"end":466},"obj":"CHEBI:16236"},{"id":"T13459","span":{"begin":493,"end":500},"obj":"CHEBI:16236"},{"id":"T13460","span":{"begin":538,"end":545},"obj":"CHEBI:16236"},{"id":"T13461","span":{"begin":557,"end":564},"obj":"UBERON:0000966"},{"id":"T13462","span":{"begin":608,"end":623},"obj":"CHEBI:38685"},{"id":"T13463","span":{"begin":807,"end":820},"obj":"CHEBI:38909"},{"id":"T13464","span":{"begin":821,"end":827},"obj":"CHEBI_CHMO_EXT:buffer_solution"},{"id":"T13465","span":{"begin":867,"end":873},"obj":"SO_EXT:sequence_altered_entity_or_alteration_process"},{"id":"T13466","span":{"begin":874,"end":880},"obj":"UBERON:0000966"},{"id":"T13467","span":{"begin":929,"end":935},"obj":"UBERON:0000966"},{"id":"T13468","span":{"begin":976,"end":983},"obj":"PR_EXT:000001195"},{"id":"T13469","span":{"begin":984,"end":994},"obj":"GO:0010467"},{"id":"T13470","span":{"begin":995,"end":1000},"obj":"CL_GO_EXT:cell"},{"id":"T13471","span":{"begin":1009,"end":1018},"obj":"SO_EXT:wild_type_entity_or_quality"},{"id":"T13472","span":{"begin":1073,"end":1081},"obj":"CHEBI:10545"},{"id":"T13473","span":{"begin":1200,"end":1204},"obj":"CHEBI_EXT:lead"},{"id":"T13474","span":{"begin":1205,"end":1212},"obj":"CHEBI_EXT:citrate"},{"id":"T13475","span":{"begin":1281,"end":1294},"obj":"CL:0000210"},{"id":"T13476","span":{"begin":1281,"end":1300},"obj":"UBERON:0001787"},{"id":"T13477","span":{"begin":1345,"end":1353},"obj":"CHEBI:10545"},{"id":"T13478","span":{"begin":1391,"end":1399},"obj":"CHEBI:10545"},{"id":"T13479","span":{"begin":1473,"end":1482},"obj":"SO_EXT:wild_type_entity_or_quality"},{"id":"T13480","span":{"begin":1487,"end":1493},"obj":"SO_EXT:sequence_altered_entity_or_alteration_process"},{"id":"T13481","span":{"begin":1494,"end":1501},"obj":"UBERON:0000966"}],"text":"The retinas were then rinsed four times for 15 min each with Sorenson's buffer (pH 7.4). They were then stained for 2 h at 4 °C with 1% osmium tetroxide in 1.5% potassium ferrocyanide. Next, the retinas were rinsed four times for 15 min in maleate buffer (pH 5.1) and then stained for 2 h at room temperature with 1% uranyl acetate in maleate buffer (pH 6.2). They were then washed four times for 15 min with maleate buffer (pH 5.1); once for 10 min with 70% ethanol; once for 10 min with 95% ethanol; and four times for 30 min with 100% ethanol. Next, the retinas were washed three times over one hour with propylene oxide and then embedded in TAAB 812 Resin (Marivac, Quebec, Canada) for 1–2 d in a 60 °C oven. Semi-thin sections were cut at a thickness of 0.5 μm and stained with 1% toluidine blue in 1% sodium borate buffer. Images of semi-thin sections from the mutant retina were taken within the ventral two-thirds of the retina where the majority of the supernumerary S-opsin–expressing cells reside. Wild-type images were taken in comparable regions. Sections for electron microscopy were cut at a thickness of 95 nm, placed on grids, and poststained with 2% uranyl acetate followed by 0.2% lead citrate. All sections were cut in a plane perpendicular to the plane of the photoreceptor layer. They were then visualized on a Jeol 1200EX electron microscope (Jeol, Tokyo, Japan). The electron microscopic images in Figure 7 derive from the ventral two-thirds of the wild-type and mutant retinas."}

{"project":"craft-ca-core-dev","denotations":[{"id":"T13377","span":{"begin":4,"end":11},"obj":"UBERON:0000966"},{"id":"T13378","span":{"begin":161,"end":183},"obj":"CHEBI:30059"},{"id":"T13379","span":{"begin":195,"end":202},"obj":"UBERON:0000966"},{"id":"T13380","span":{"begin":459,"end":466},"obj":"CHEBI:16236"},{"id":"T13381","span":{"begin":493,"end":500},"obj":"CHEBI:16236"},{"id":"T13382","span":{"begin":538,"end":545},"obj":"CHEBI:16236"},{"id":"T13383","span":{"begin":557,"end":564},"obj":"UBERON:0000966"},{"id":"T13384","span":{"begin":608,"end":623},"obj":"CHEBI:38685"},{"id":"T13385","span":{"begin":807,"end":820},"obj":"CHEBI:38909"},{"id":"T13386","span":{"begin":874,"end":880},"obj":"UBERON:0000966"},{"id":"T13387","span":{"begin":929,"end":935},"obj":"UBERON:0000966"},{"id":"T13388","span":{"begin":976,"end":983},"obj":"PR:000001195"},{"id":"T13389","span":{"begin":984,"end":994},"obj":"GO:0010467"},{"id":"T13390","span":{"begin":1073,"end":1081},"obj":"CHEBI:10545"},{"id":"T13391","span":{"begin":1281,"end":1294},"obj":"CL:0000210"},{"id":"T13392","span":{"begin":1281,"end":1300},"obj":"UBERON:0001787"},{"id":"T13393","span":{"begin":1345,"end":1353},"obj":"CHEBI:10545"},{"id":"T13394","span":{"begin":1391,"end":1399},"obj":"CHEBI:10545"},{"id":"T13395","span":{"begin":1494,"end":1501},"obj":"UBERON:0000966"}],"text":"The retinas were then rinsed four times for 15 min each with Sorenson's buffer (pH 7.4). They were then stained for 2 h at 4 °C with 1% osmium tetroxide in 1.5% potassium ferrocyanide. Next, the retinas were rinsed four times for 15 min in maleate buffer (pH 5.1) and then stained for 2 h at room temperature with 1% uranyl acetate in maleate buffer (pH 6.2). They were then washed four times for 15 min with maleate buffer (pH 5.1); once for 10 min with 70% ethanol; once for 10 min with 95% ethanol; and four times for 30 min with 100% ethanol. Next, the retinas were washed three times over one hour with propylene oxide and then embedded in TAAB 812 Resin (Marivac, Quebec, Canada) for 1–2 d in a 60 °C oven. Semi-thin sections were cut at a thickness of 0.5 μm and stained with 1% toluidine blue in 1% sodium borate buffer. Images of semi-thin sections from the mutant retina were taken within the ventral two-thirds of the retina where the majority of the supernumerary S-opsin–expressing cells reside. Wild-type images were taken in comparable regions. Sections for electron microscopy were cut at a thickness of 95 nm, placed on grids, and poststained with 2% uranyl acetate followed by 0.2% lead citrate. All sections were cut in a plane perpendicular to the plane of the photoreceptor layer. They were then visualized on a Jeol 1200EX electron microscope (Jeol, Tokyo, Japan). The electron microscopic images in Figure 7 derive from the ventral two-thirds of the wild-type and mutant retinas."}