Id |
Subject |
Object |
Predicate |
Lexical cue |
T13140 |
0-3 |
IN |
denotes |
For |
T13141 |
41-49 |
VBN |
denotes |
prepared |
T13142 |
4-12 |
NN |
denotes |
antibody |
T13143 |
13-21 |
NN |
denotes |
staining |
T13144 |
21-23 |
, |
denotes |
, |
T13145 |
23-35 |
NNS |
denotes |
cryosections |
T13146 |
36-40 |
VBD |
denotes |
were |
T13147 |
50-53 |
CC |
denotes |
and |
T13148 |
54-61 |
VBN |
denotes |
stained |
T13149 |
62-64 |
IN |
denotes |
as |
T13150 |
65-74 |
VBN |
denotes |
described |
T13151 |
75-85 |
RB |
denotes |
previously |
T13152 |
86-87 |
-LRB- |
denotes |
[ |
T13153 |
87-89 |
CD |
denotes |
63 |
T13154 |
89-90 |
-RRB- |
denotes |
] |
T13155 |
90-91 |
. |
denotes |
. |
T13156 |
91-298 |
sentence |
denotes |
Primary antibodies used were a polyclonal anti-blue opsin raised in rabbit (1:300; Chemicon International, Temecula, California, United States; AB5407) and a mouse monoclonal anti-rhodopsin (1:200; rho4D2). |
T13157 |
92-99 |
JJ |
denotes |
Primary |
T13158 |
100-110 |
NNS |
denotes |
antibodies |
T13159 |
116-120 |
VBD |
denotes |
were |
T13160 |
111-115 |
VBN |
denotes |
used |
T13161 |
121-122 |
DT |
denotes |
a |
T13162 |
144-149 |
NN |
denotes |
opsin |
T13163 |
123-133 |
JJ |
denotes |
polyclonal |
T13164 |
134-143 |
JJ |
denotes |
anti-blue |
T13165 |
150-156 |
VBN |
denotes |
raised |
T13166 |
157-159 |
IN |
denotes |
in |
T13167 |
160-166 |
NN |
denotes |
rabbit |
T13168 |
167-168 |
-LRB- |
denotes |
( |
T13169 |
236-242 |
NN |
denotes |
AB5407 |
T13170 |
168-169 |
CD |
denotes |
1 |
T13171 |
169-170 |
SYM |
denotes |
: |
T13172 |
170-173 |
CD |
denotes |
300 |
T13173 |
173-174 |
: |
denotes |
; |
T13174 |
175-183 |
NNP |
denotes |
Chemicon |
T13175 |
184-197 |
NNP |
denotes |
International |
T13176 |
197-199 |
, |
denotes |
, |
T13177 |
199-207 |
NNP |
denotes |
Temecula |
T13178 |
207-209 |
, |
denotes |
, |
T13179 |
209-219 |
NNP |
denotes |
California |
T13180 |
219-221 |
, |
denotes |
, |
T13181 |
221-227 |
NNP |
denotes |
United |
T13182 |
228-234 |
NNP |
denotes |
States |
T13183 |
234-235 |
: |
denotes |
; |
T13184 |
242-243 |
-RRB- |
denotes |
) |
T13185 |
244-247 |
CC |
denotes |
and |
T13186 |
248-249 |
DT |
denotes |
a |
T13187 |
267-281 |
NN |
denotes |
anti-rhodopsin |
T13188 |
250-255 |
NN |
denotes |
mouse |
T13189 |
256-266 |
JJ |
denotes |
monoclonal |
T13190 |
282-283 |
-LRB- |
denotes |
( |
T13191 |
290-296 |
NN |
denotes |
rho4D2 |
T13192 |
283-284 |
CD |
denotes |
1 |
T13193 |
284-285 |
SYM |
denotes |
: |
T13194 |
285-288 |
CD |
denotes |
200 |
T13195 |
288-289 |
: |
denotes |
; |
T13196 |
296-297 |
-RRB- |
denotes |
) |
T13197 |
297-298 |
. |
denotes |
. |
T13198 |
298-458 |
sentence |
denotes |
Secondary antibodies used were Cy2- or Cy3-conjugated goat anti-rabbit and anti-mouse (1:500; Jackson Immunologicals, West Grove, Pennsylvania, United States). |
T13199 |
299-308 |
JJ |
denotes |
Secondary |
T13200 |
309-319 |
NNS |
denotes |
antibodies |
T13201 |
325-329 |
VBD |
denotes |
were |
T13202 |
320-324 |
VBN |
denotes |
used |
T13203 |
330-333 |
NN |
denotes |
Cy2 |
T13204 |
342-352 |
VBN |
denotes |
conjugated |
T13205 |
333-334 |
HYPH |
denotes |
- |
T13206 |
335-337 |
CC |
denotes |
or |
T13207 |
338-341 |
NN |
denotes |
Cy3 |
T13208 |
341-342 |
HYPH |
denotes |
- |
T13209 |
358-369 |
NN |
denotes |
anti-rabbit |
T13210 |
353-357 |
NN |
denotes |
goat |
T13211 |
370-373 |
CC |
denotes |
and |
T13212 |
374-384 |
NN |
denotes |
anti-mouse |
T13213 |
385-386 |
-LRB- |
denotes |
( |
T13214 |
401-415 |
NNPS |
denotes |
Immunologicals |
T13215 |
386-387 |
CD |
denotes |
1 |
T13216 |
387-388 |
SYM |
denotes |
: |
T13217 |
388-391 |
CD |
denotes |
500 |
T13218 |
391-392 |
: |
denotes |
; |
T13219 |
393-400 |
NNP |
denotes |
Jackson |
T13220 |
415-417 |
, |
denotes |
, |
T13221 |
417-421 |
NNP |
denotes |
West |
T13222 |
422-427 |
NNP |
denotes |
Grove |
T13223 |
427-429 |
, |
denotes |
, |
T13224 |
429-441 |
NNP |
denotes |
Pennsylvania |
T13225 |
441-443 |
, |
denotes |
, |
T13226 |
443-449 |
NNP |
denotes |
United |
T13227 |
450-456 |
NNP |
denotes |
States |
T13228 |
456-457 |
-RRB- |
denotes |
) |
T13229 |
457-458 |
. |
denotes |
. |
T13230 |
458-643 |
sentence |
denotes |
Following antibody staining, 4′-DAPI was applied to stain nuclei (Sigma), and the sections were coverslipped and mounted in Gel/Mount (Biomeda, Foster City, California, United States). |
T13231 |
459-468 |
VBG |
denotes |
Following |
T13232 |
500-507 |
VBN |
denotes |
applied |
T13233 |
469-477 |
NN |
denotes |
antibody |
T13234 |
478-486 |
NN |
denotes |
staining |
T13235 |
486-488 |
, |
denotes |
, |
T13236 |
488-489 |
CD |
denotes |
4 |
T13237 |
491-495 |
NN |
denotes |
DAPI |
T13238 |
489-490 |
SYM |
denotes |
′ |
T13239 |
490-491 |
HYPH |
denotes |
- |
T13240 |
496-499 |
VBD |
denotes |
was |
T13241 |
508-510 |
TO |
denotes |
to |
T13242 |
511-516 |
VB |
denotes |
stain |
T13243 |
517-523 |
NNS |
denotes |
nuclei |
T13244 |
524-525 |
-LRB- |
denotes |
( |
T13245 |
525-530 |
NNP |
denotes |
Sigma |
T13246 |
530-531 |
-RRB- |
denotes |
) |
T13247 |
531-533 |
, |
denotes |
, |
T13248 |
533-536 |
CC |
denotes |
and |
T13249 |
537-540 |
DT |
denotes |
the |
T13250 |
541-549 |
NNS |
denotes |
sections |
T13251 |
555-567 |
VBN |
denotes |
coverslipped |
T13252 |
550-554 |
VBD |
denotes |
were |
T13253 |
568-571 |
CC |
denotes |
and |
T13254 |
572-579 |
VBN |
denotes |
mounted |
T13255 |
580-582 |
IN |
denotes |
in |
T13256 |
583-586 |
NN |
denotes |
Gel |
T13257 |
587-592 |
NN |
denotes |
Mount |
T13258 |
586-587 |
HYPH |
denotes |
/ |
T13259 |
593-594 |
-LRB- |
denotes |
( |
T13260 |
594-601 |
NNP |
denotes |
Biomeda |
T13261 |
601-603 |
, |
denotes |
, |
T13262 |
603-609 |
NNP |
denotes |
Foster |
T13263 |
610-614 |
NNP |
denotes |
City |
T13264 |
614-616 |
, |
denotes |
, |
T13265 |
616-626 |
NNP |
denotes |
California |
T13266 |
626-628 |
, |
denotes |
, |
T13267 |
628-634 |
NNP |
denotes |
United |
T13268 |
635-641 |
NNP |
denotes |
States |
T13269 |
641-642 |
-RRB- |
denotes |
) |
T13270 |
642-643 |
. |
denotes |
. |
R8803 |
T13140 |
T13141 |
prep |
For,prepared |
R8804 |
T13142 |
T13143 |
compound |
antibody,staining |
R8805 |
T13143 |
T13140 |
pobj |
staining,For |
R8806 |
T13144 |
T13141 |
punct |
", ",prepared |
R8807 |
T13145 |
T13141 |
nsubjpass |
cryosections,prepared |
R8808 |
T13146 |
T13141 |
auxpass |
were,prepared |
R8809 |
T13147 |
T13141 |
cc |
and,prepared |
R8810 |
T13148 |
T13141 |
conj |
stained,prepared |
R8811 |
T13149 |
T13150 |
mark |
as,described |
R8812 |
T13150 |
T13148 |
advcl |
described,stained |
R8813 |
T13151 |
T13150 |
advmod |
previously,described |
R8814 |
T13152 |
T13153 |
punct |
[,63 |
R8815 |
T13153 |
T13148 |
parataxis |
63,stained |
R8816 |
T13154 |
T13153 |
punct |
],63 |
R8817 |
T13155 |
T13141 |
punct |
.,prepared |
R8818 |
T13157 |
T13158 |
amod |
Primary,antibodies |
R8819 |
T13158 |
T13159 |
nsubj |
antibodies,were |
R8820 |
T13160 |
T13158 |
acl |
used,antibodies |
R8821 |
T13161 |
T13162 |
det |
a,opsin |
R8822 |
T13162 |
T13159 |
attr |
opsin,were |
R8823 |
T13163 |
T13162 |
amod |
polyclonal,opsin |
R8824 |
T13164 |
T13162 |
amod |
anti-blue,opsin |
R8825 |
T13165 |
T13162 |
acl |
raised,opsin |
R8826 |
T13166 |
T13165 |
prep |
in,raised |
R8827 |
T13167 |
T13166 |
pobj |
rabbit,in |
R8828 |
T13168 |
T13169 |
punct |
(,AB5407 |
R8829 |
T13169 |
T13167 |
parataxis |
AB5407,rabbit |
R8830 |
T13170 |
T13169 |
dep |
1,AB5407 |
R8831 |
T13171 |
T13172 |
punct |
:,300 |
R8832 |
T13172 |
T13170 |
prep |
300,1 |
R8833 |
T13173 |
T13169 |
punct |
;,AB5407 |
R8834 |
T13174 |
T13175 |
compound |
Chemicon,International |
R8835 |
T13175 |
T13169 |
dep |
International,AB5407 |
R8836 |
T13176 |
T13175 |
punct |
", ",International |
R8837 |
T13177 |
T13175 |
npadvmod |
Temecula,International |
R8838 |
T13178 |
T13175 |
punct |
", ",International |
R8839 |
T13179 |
T13175 |
npadvmod |
California,International |
R8840 |
T13180 |
T13175 |
punct |
", ",International |
R8841 |
T13181 |
T13182 |
compound |
United,States |
R8842 |
T13182 |
T13175 |
appos |
States,International |
R8843 |
T13183 |
T13169 |
punct |
;,AB5407 |
R8844 |
T13184 |
T13169 |
punct |
),AB5407 |
R8845 |
T13185 |
T13162 |
cc |
and,opsin |
R8846 |
T13186 |
T13187 |
det |
a,anti-rhodopsin |
R8847 |
T13187 |
T13162 |
conj |
anti-rhodopsin,opsin |
R8848 |
T13188 |
T13187 |
nmod |
mouse,anti-rhodopsin |
R8849 |
T13189 |
T13187 |
amod |
monoclonal,anti-rhodopsin |
R8850 |
T13190 |
T13191 |
punct |
(,rho4D2 |
R8851 |
T13191 |
T13187 |
parataxis |
rho4D2,anti-rhodopsin |
R8852 |
T13192 |
T13191 |
dep |
1,rho4D2 |
R8853 |
T13193 |
T13194 |
punct |
:,200 |
R8854 |
T13194 |
T13192 |
prep |
200,1 |
R8855 |
T13195 |
T13191 |
punct |
;,rho4D2 |
R8856 |
T13196 |
T13191 |
punct |
),rho4D2 |
R8857 |
T13197 |
T13159 |
punct |
.,were |
R8858 |
T13199 |
T13200 |
amod |
Secondary,antibodies |
R8859 |
T13200 |
T13201 |
nsubj |
antibodies,were |
R8860 |
T13202 |
T13200 |
acl |
used,antibodies |
R8861 |
T13203 |
T13204 |
npadvmod |
Cy2,conjugated |
R8862 |
T13204 |
T13209 |
amod |
conjugated,anti-rabbit |
R8863 |
T13205 |
T13203 |
punct |
-,Cy2 |
R8864 |
T13206 |
T13203 |
cc |
or,Cy2 |
R8865 |
T13207 |
T13203 |
conj |
Cy3,Cy2 |
R8866 |
T13208 |
T13204 |
punct |
-,conjugated |
R8867 |
T13209 |
T13201 |
attr |
anti-rabbit,were |
R8868 |
T13210 |
T13209 |
compound |
goat,anti-rabbit |
R8869 |
T13211 |
T13209 |
cc |
and,anti-rabbit |
R8870 |
T13212 |
T13209 |
conj |
anti-mouse,anti-rabbit |
R8871 |
T13213 |
T13214 |
punct |
(,Immunologicals |
R8872 |
T13214 |
T13209 |
parataxis |
Immunologicals,anti-rabbit |
R8873 |
T13215 |
T13214 |
dep |
1,Immunologicals |
R8874 |
T13216 |
T13217 |
punct |
:,500 |
R8875 |
T13217 |
T13215 |
prep |
500,1 |
R8876 |
T13218 |
T13214 |
punct |
;,Immunologicals |
R8877 |
T13219 |
T13214 |
compound |
Jackson,Immunologicals |
R8878 |
T13220 |
T13214 |
punct |
", ",Immunologicals |
R8879 |
T13221 |
T13222 |
compound |
West,Grove |
R8880 |
T13222 |
T13214 |
npadvmod |
Grove,Immunologicals |
R8881 |
T13223 |
T13214 |
punct |
", ",Immunologicals |
R8882 |
T13224 |
T13214 |
npadvmod |
Pennsylvania,Immunologicals |
R8883 |
T13225 |
T13214 |
punct |
", ",Immunologicals |
R8884 |
T13226 |
T13227 |
compound |
United,States |
R8885 |
T13227 |
T13214 |
npadvmod |
States,Immunologicals |
R8886 |
T13228 |
T13214 |
punct |
),Immunologicals |
R8887 |
T13229 |
T13201 |
punct |
.,were |
R8888 |
T13231 |
T13232 |
prep |
Following,applied |
R8889 |
T13233 |
T13234 |
compound |
antibody,staining |
R8890 |
T13234 |
T13231 |
pobj |
staining,Following |
R8891 |
T13235 |
T13232 |
punct |
", ",applied |
R8892 |
T13236 |
T13237 |
nummod |
4,DAPI |
R8893 |
T13237 |
T13232 |
nsubjpass |
DAPI,applied |
R8894 |
T13238 |
T13236 |
punct |
′,4 |
R8895 |
T13239 |
T13237 |
punct |
-,DAPI |
R8896 |
T13240 |
T13232 |
auxpass |
was,applied |
R8897 |
T13241 |
T13242 |
aux |
to,stain |
R8898 |
T13242 |
T13232 |
advcl |
stain,applied |
R8899 |
T13243 |
T13242 |
dobj |
nuclei,stain |
R8900 |
T13244 |
T13245 |
punct |
(,Sigma |
R8901 |
T13245 |
T13232 |
parataxis |
Sigma,applied |
R8902 |
T13246 |
T13245 |
punct |
),Sigma |
R8903 |
T13247 |
T13232 |
punct |
", ",applied |
R8904 |
T13248 |
T13232 |
cc |
and,applied |
R8905 |
T13249 |
T13250 |
det |
the,sections |
R8906 |
T13250 |
T13251 |
nsubjpass |
sections,coverslipped |
R8907 |
T13251 |
T13232 |
conj |
coverslipped,applied |
R8908 |
T13252 |
T13251 |
auxpass |
were,coverslipped |
R8909 |
T13253 |
T13251 |
cc |
and,coverslipped |
R8910 |
T13254 |
T13251 |
conj |
mounted,coverslipped |
R8911 |
T13255 |
T13254 |
prep |
in,mounted |
R8912 |
T13256 |
T13257 |
compound |
Gel,Mount |
R8913 |
T13257 |
T13255 |
pobj |
Mount,in |
R8914 |
T13258 |
T13257 |
punct |
/,Mount |
R8915 |
T13259 |
T13260 |
punct |
(,Biomeda |
R8916 |
T13260 |
T13257 |
parataxis |
Biomeda,Mount |
R8917 |
T13261 |
T13260 |
punct |
", ",Biomeda |
R8918 |
T13262 |
T13263 |
compound |
Foster,City |
R8919 |
T13263 |
T13260 |
npadvmod |
City,Biomeda |
R8920 |
T13264 |
T13260 |
punct |
", ",Biomeda |
R8921 |
T13265 |
T13260 |
npadvmod |
California,Biomeda |
R8922 |
T13266 |
T13260 |
punct |
", ",Biomeda |
R8923 |
T13267 |
T13268 |
compound |
United,States |
R8924 |
T13268 |
T13260 |
npadvmod |
States,Biomeda |
R8925 |
T13269 |
T13260 |
punct |
),Biomeda |
R8926 |
T13270 |
T13232 |
punct |
.,applied |