PMC:1183529 / 28710-29778 JSONTXT

{"project":"craft-ca-core-dev","denotations":[{"id":"T15185","span":{"begin":6,"end":17},"obj":"GO:0007565"},{"id":"T15186","span":{"begin":60,"end":67},"obj":"UBERON:0000922"},{"id":"T15187","span":{"begin":69,"end":76},"obj":"UBERON:0000922"},{"id":"T15188","span":{"begin":156,"end":163},"obj":"UBERON:0000922"},{"id":"T15189","span":{"begin":231,"end":240},"obj":"UBERON:0001103"},{"id":"T15190","span":{"begin":242,"end":247},"obj":"UBERON:0002048"},{"id":"T15191","span":{"begin":253,"end":258},"obj":"UBERON:0000948"},{"id":"T15192","span":{"begin":278,"end":283},"obj":"UBERON:0002048"},{"id":"T15193","span":{"begin":288,"end":309},"obj":"UBERON:0007196"},{"id":"T15194","span":{"begin":342,"end":352},"obj":"UBERON:0001103"},{"id":"T15195","span":{"begin":378,"end":386},"obj":"UBERON:0000915"},{"id":"T15196","span":{"begin":387,"end":393},"obj":"UBERON:0000479"},{"id":"T15197","span":{"begin":415,"end":422},"obj":"UBERON:0000922"},{"id":"T15198","span":{"begin":428,"end":432},"obj":"UBERON:0002048"},{"id":"T15199","span":{"begin":450,"end":455},"obj":"UBERON:0002048"},{"id":"T15200","span":{"begin":482,"end":489},"obj":"UBERON:0000922"},{"id":"T15201","span":{"begin":636,"end":644},"obj":"CHEBI:33284"},{"id":"T15202","span":{"begin":645,"end":652},"obj":"CHEBI:60004"},{"id":"T15203","span":{"begin":744,"end":750},"obj":"NCBITaxon:27592"},{"id":"T15204","span":{"begin":751,"end":756},"obj":"UBERON:0001977"},{"id":"T15205","span":{"begin":794,"end":804},"obj":"CHEBI:17334"},{"id":"T15206","span":{"begin":817,"end":829},"obj":"CHEBI:17076"},{"id":"T15207","span":{"begin":847,"end":861},"obj":"CHEBI:2682"},{"id":"T15208","span":{"begin":863,"end":867},"obj":"UBERON:0002048"},{"id":"T15209","span":{"begin":914,"end":917},"obj":"CHEBI:16526"}],"text":"Timed pregnancies were set up for collection of E11.5–E17.5 embryos. Embryos were fixed, dehydrated, and embedded in paraffin prior to sectioning. In older embryos, a median sternotomy was performed under microscopic guidance, and diaphragm, lungs, and heart were examined. The lungs and tracheobronchial tree were removed and weighed. Whole diaphragms were isolated from fixed thoracic tissue from E15.5 and E17.5 embryos. For lung explant culture, lungs were dissected from fresh embryos at E11.5 and E12.5 and placed on porous 24-mm (0.4-μ) polyester membranes floated in wells containing 2 ml of Dulbecco's modified Eagle's medium, nutrient mixture F-12 (11039–021, Gibco, San Diego, California, United States), supplemented with 10% fetal bovine serum, 0.3 mg/ml L-glutamine, 100 units/ml penicillin, 100 mcg/ml streptomycin, and 0.25 mcg/ml amphotericin B. Lung explants were cultured at 37 °C in 95% air/5% CO2 for up to 5 d. They were photographed daily with a dissecting microscope (MZ12.5, Leica, Wetzlar, Germany) equipped with a Leica DC500 digital camera."}

{"project":"craft-ca-core-ex-dev","denotations":[{"id":"T15242","span":{"begin":6,"end":17},"obj":"GO:0007565"},{"id":"T15243","span":{"begin":60,"end":67},"obj":"UBERON:0000922"},{"id":"T15244","span":{"begin":69,"end":76},"obj":"UBERON:0000922"},{"id":"T15245","span":{"begin":156,"end":163},"obj":"UBERON:0000922"},{"id":"T15246","span":{"begin":231,"end":240},"obj":"UBERON:0001103"},{"id":"T15247","span":{"begin":242,"end":247},"obj":"UBERON:0002048"},{"id":"T15248","span":{"begin":253,"end":258},"obj":"UBERON:0000948"},{"id":"T15249","span":{"begin":278,"end":283},"obj":"UBERON:0002048"},{"id":"T15250","span":{"begin":288,"end":309},"obj":"UBERON:0007196"},{"id":"T15251","span":{"begin":342,"end":352},"obj":"UBERON:0001103"},{"id":"T15252","span":{"begin":378,"end":386},"obj":"UBERON:0000915"},{"id":"T15253","span":{"begin":387,"end":393},"obj":"UBERON:0000479"},{"id":"T15254","span":{"begin":415,"end":422},"obj":"UBERON:0000922"},{"id":"T15255","span":{"begin":428,"end":432},"obj":"UBERON:0002048"},{"id":"T15256","span":{"begin":450,"end":455},"obj":"UBERON:0002048"},{"id":"T15257","span":{"begin":482,"end":489},"obj":"UBERON:0000922"},{"id":"T15258","span":{"begin":544,"end":553},"obj":"CHEBI_EXT:polyester"},{"id":"T15259","span":{"begin":636,"end":644},"obj":"CHEBI_EXT:33284"},{"id":"T15260","span":{"begin":645,"end":652},"obj":"CHEBI:60004"},{"id":"T15261","span":{"begin":744,"end":750},"obj":"NCBITaxon:27592"},{"id":"T15262","span":{"begin":751,"end":756},"obj":"UBERON:0001977"},{"id":"T15263","span":{"begin":768,"end":779},"obj":"CHEBI_EXT:L_glutamine"},{"id":"T15264","span":{"begin":770,"end":779},"obj":"CHEBI_SO_EXT:glutamine"},{"id":"T15265","span":{"begin":794,"end":804},"obj":"CHEBI:17334"},{"id":"T15266","span":{"begin":817,"end":829},"obj":"CHEBI:17076"},{"id":"T15267","span":{"begin":847,"end":861},"obj":"CHEBI:2682"},{"id":"T15268","span":{"begin":863,"end":867},"obj":"UBERON:0002048"},{"id":"T15269","span":{"begin":914,"end":917},"obj":"CHEBI:16526"}],"text":"Timed pregnancies were set up for collection of E11.5–E17.5 embryos. Embryos were fixed, dehydrated, and embedded in paraffin prior to sectioning. In older embryos, a median sternotomy was performed under microscopic guidance, and diaphragm, lungs, and heart were examined. The lungs and tracheobronchial tree were removed and weighed. Whole diaphragms were isolated from fixed thoracic tissue from E15.5 and E17.5 embryos. For lung explant culture, lungs were dissected from fresh embryos at E11.5 and E12.5 and placed on porous 24-mm (0.4-μ) polyester membranes floated in wells containing 2 ml of Dulbecco's modified Eagle's medium, nutrient mixture F-12 (11039–021, Gibco, San Diego, California, United States), supplemented with 10% fetal bovine serum, 0.3 mg/ml L-glutamine, 100 units/ml penicillin, 100 mcg/ml streptomycin, and 0.25 mcg/ml amphotericin B. Lung explants were cultured at 37 °C in 95% air/5% CO2 for up to 5 d. They were photographed daily with a dissecting microscope (MZ12.5, Leica, Wetzlar, Germany) equipped with a Leica DC500 digital camera."}

{"project":"craft-sa-dev","denotations":[{"id":"T15319","span":{"begin":0,"end":5},"obj":"JJ"},{"id":"T15320","span":{"begin":6,"end":17},"obj":"NNS"},{"id":"T15321","span":{"begin":23,"end":26},"obj":"VBN"},{"id":"T15322","span":{"begin":18,"end":22},"obj":"VBD"},{"id":"T15323","span":{"begin":27,"end":29},"obj":"RP"},{"id":"T15324","span":{"begin":30,"end":33},"obj":"IN"},{"id":"T15325","span":{"begin":34,"end":44},"obj":"NN"},{"id":"T15326","span":{"begin":45,"end":47},"obj":"IN"},{"id":"T15327","span":{"begin":48,"end":53},"obj":"NN"},{"id":"T15328","span":{"begin":60,"end":67},"obj":"NNS"},{"id":"T15329","span":{"begin":53,"end":54},"obj":"SYM"},{"id":"T15330","span":{"begin":54,"end":59},"obj":"NN"},{"id":"T15331","span":{"begin":67,"end":68},"obj":"."},{"id":"T15332","span":{"begin":68,"end":146},"obj":"sentence"},{"id":"T15333","span":{"begin":69,"end":76},"obj":"NNS"},{"id":"T15334","span":{"begin":82,"end":87},"obj":"VBN"},{"id":"T15335","span":{"begin":77,"end":81},"obj":"VBD"},{"id":"T15336","span":{"begin":87,"end":89},"obj":","},{"id":"T15337","span":{"begin":89,"end":99},"obj":"VBN"},{"id":"T15338","span":{"begin":99,"end":101},"obj":","},{"id":"T15339","span":{"begin":101,"end":104},"obj":"CC"},{"id":"T15340","span":{"begin":105,"end":113},"obj":"VBN"},{"id":"T15341","span":{"begin":114,"end":116},"obj":"IN"},{"id":"T15342","span":{"begin":117,"end":125},"obj":"NN"},{"id":"T15343","span":{"begin":126,"end":131},"obj":"JJ"},{"id":"T15344","span":{"begin":132,"end":134},"obj":"IN"},{"id":"T15345","span":{"begin":135,"end":145},"obj":"NN"},{"id":"T15346","span":{"begin":145,"end":146},"obj":"."},{"id":"T15347","span":{"begin":146,"end":273},"obj":"sentence"},{"id":"T15348","span":{"begin":147,"end":149},"obj":"IN"},{"id":"T15349","span":{"begin":189,"end":198},"obj":"VBN"},{"id":"T15350","span":{"begin":150,"end":155},"obj":"JJR"},{"id":"T15351","span":{"begin":156,"end":163},"obj":"NNS"},{"id":"T15352","span":{"begin":163,"end":165},"obj":","},{"id":"T15353","span":{"begin":165,"end":166},"obj":"DT"},{"id":"T15354","span":{"begin":174,"end":184},"obj":"NN"},{"id":"T15355","span":{"begin":167,"end":173},"obj":"JJ"},{"id":"T15356","span":{"begin":185,"end":188},"obj":"VBD"},{"id":"T15357","span":{"begin":199,"end":204},"obj":"IN"},{"id":"T15358","span":{"begin":205,"end":216},"obj":"JJ"},{"id":"T15359","span":{"begin":217,"end":225},"obj":"NN"},{"id":"T15360","span":{"begin":225,"end":227},"obj":","},{"id":"T15361","span":{"begin":227,"end":230},"obj":"CC"},{"id":"T15362","span":{"begin":231,"end":240},"obj":"NN"},{"id":"T15363","span":{"begin":264,"end":272},"obj":"VBN"},{"id":"T15364","span":{"begin":240,"end":242},"obj":","},{"id":"T15365","span":{"begin":242,"end":247},"obj":"NNS"},{"id":"T15366","span":{"begin":247,"end":249},"obj":","},{"id":"T15367","span":{"begin":249,"end":252},"obj":"CC"},{"id":"T15368","span":{"begin":253,"end":258},"obj":"NN"},{"id":"T15369","span":{"begin":259,"end":263},"obj":"VBD"},{"id":"T15370","span":{"begin":272,"end":273},"obj":"."},{"id":"T15371","span":{"begin":273,"end":335},"obj":"sentence"},{"id":"T15372","span":{"begin":274,"end":277},"obj":"DT"},{"id":"T15373","span":{"begin":278,"end":283},"obj":"NNS"},{"id":"T15374","span":{"begin":315,"end":322},"obj":"VBN"},{"id":"T15375","span":{"begin":284,"end":287},"obj":"CC"},{"id":"T15376","span":{"begin":288,"end":304},"obj":"JJ"},{"id":"T15377","span":{"begin":305,"end":309},"obj":"NN"},{"id":"T15378","span":{"begin":310,"end":314},"obj":"VBD"},{"id":"T15379","span":{"begin":323,"end":326},"obj":"CC"},{"id":"T15380","span":{"begin":327,"end":334},"obj":"VBN"},{"id":"T15381","span":{"begin":334,"end":335},"obj":"."},{"id":"T15382","span":{"begin":335,"end":423},"obj":"sentence"},{"id":"T15383","span":{"begin":336,"end":341},"obj":"JJ"},{"id":"T15384","span":{"begin":342,"end":352},"obj":"NNS"},{"id":"T15385","span":{"begin":358,"end":366},"obj":"VBN"},{"id":"T15386","span":{"begin":353,"end":357},"obj":"VBD"},{"id":"T15387","span":{"begin":367,"end":371},"obj":"IN"},{"id":"T15388","span":{"begin":372,"end":377},"obj":"VBN"},{"id":"T15389","span":{"begin":387,"end":393},"obj":"NN"},{"id":"T15390","span":{"begin":378,"end":386},"obj":"JJ"},{"id":"T15391","span":{"begin":394,"end":398},"obj":"IN"},{"id":"T15392","span":{"begin":399,"end":404},"obj":"NN"},{"id":"T15393","span":{"begin":415,"end":422},"obj":"NNS"},{"id":"T15394","span":{"begin":405,"end":408},"obj":"CC"},{"id":"T15395","span":{"begin":409,"end":414},"obj":"NN"},{"id":"T15396","span":{"begin":422,"end":423},"obj":"."},{"id":"T15397","span":{"begin":423,"end":862},"obj":"sentence"},{"id":"T15398","span":{"begin":424,"end":427},"obj":"IN"},{"id":"T15399","span":{"begin":461,"end":470},"obj":"VBN"},{"id":"T15400","span":{"begin":428,"end":432},"obj":"NN"},{"id":"T15401","span":{"begin":441,"end":448},"obj":"NN"},{"id":"T15402","span":{"begin":433,"end":440},"obj":"NN"},{"id":"T15403","span":{"begin":448,"end":450},"obj":","},{"id":"T15404","span":{"begin":450,"end":455},"obj":"NNS"},{"id":"T15405","span":{"begin":456,"end":460},"obj":"VBD"},{"id":"T15406","span":{"begin":471,"end":475},"obj":"IN"},{"id":"T15407","span":{"begin":476,"end":481},"obj":"JJ"},{"id":"T15408","span":{"begin":482,"end":489},"obj":"NNS"},{"id":"T15409","span":{"begin":490,"end":492},"obj":"IN"},{"id":"T15410","span":{"begin":493,"end":498},"obj":"NN"},{"id":"T15411","span":{"begin":499,"end":502},"obj":"CC"},{"id":"T15412","span":{"begin":503,"end":508},"obj":"NN"},{"id":"T15413","span":{"begin":509,"end":512},"obj":"CC"},{"id":"T15414","span":{"begin":513,"end":519},"obj":"VBN"},{"id":"T15415","span":{"begin":520,"end":522},"obj":"IN"},{"id":"T15416","span":{"begin":523,"end":529},"obj":"JJ"},{"id":"T15417","span":{"begin":554,"end":563},"obj":"NNS"},{"id":"T15418","span":{"begin":530,"end":532},"obj":"CD"},{"id":"T15419","span":{"begin":533,"end":535},"obj":"NN"},{"id":"T15420","span":{"begin":532,"end":533},"obj":"HYPH"},{"id":"T15421","span":{"begin":536,"end":537},"obj":"-LRB-"},{"id":"T15422","span":{"begin":541,"end":542},"obj":"NN"},{"id":"T15423","span":{"begin":537,"end":540},"obj":"CD"},{"id":"T15424","span":{"begin":540,"end":541},"obj":"HYPH"},{"id":"T15425","span":{"begin":542,"end":543},"obj":"-RRB-"},{"id":"T15426","span":{"begin":544,"end":553},"obj":"NN"},{"id":"T15427","span":{"begin":564,"end":571},"obj":"VBN"},{"id":"T15428","span":{"begin":572,"end":574},"obj":"IN"},{"id":"T15429","span":{"begin":575,"end":580},"obj":"NNS"},{"id":"T15430","span":{"begin":581,"end":591},"obj":"VBG"},{"id":"T15431","span":{"begin":592,"end":593},"obj":"CD"},{"id":"T15432","span":{"begin":594,"end":596},"obj":"NN"},{"id":"T15433","span":{"begin":597,"end":599},"obj":"IN"},{"id":"T15434","span":{"begin":600,"end":608},"obj":"NNP"},{"id":"T15435","span":{"begin":628,"end":634},"obj":"NN"},{"id":"T15436","span":{"begin":608,"end":610},"obj":"POS"},{"id":"T15437","span":{"begin":611,"end":619},"obj":"VBN"},{"id":"T15438","span":{"begin":620,"end":625},"obj":"NNP"},{"id":"T15439","span":{"begin":625,"end":627},"obj":"POS"},{"id":"T15440","span":{"begin":634,"end":636},"obj":","},{"id":"T15441","span":{"begin":636,"end":644},"obj":"NN"},{"id":"T15442","span":{"begin":645,"end":652},"obj":"NN"},{"id":"T15443","span":{"begin":653,"end":654},"obj":"NN"},{"id":"T15444","span":{"begin":654,"end":655},"obj":"HYPH"},{"id":"T15445","span":{"begin":655,"end":657},"obj":"CD"},{"id":"T15446","span":{"begin":658,"end":659},"obj":"-LRB-"},{"id":"T15447","span":{"begin":670,"end":675},"obj":"NNP"},{"id":"T15448","span":{"begin":659,"end":664},"obj":"CD"},{"id":"T15449","span":{"begin":665,"end":668},"obj":"CD"},{"id":"T15450","span":{"begin":664,"end":665},"obj":"HYPH"},{"id":"T15451","span":{"begin":668,"end":670},"obj":","},{"id":"T15452","span":{"begin":675,"end":677},"obj":","},{"id":"T15453","span":{"begin":677,"end":680},"obj":"NNP"},{"id":"T15454","span":{"begin":681,"end":686},"obj":"NNP"},{"id":"T15455","span":{"begin":686,"end":688},"obj":","},{"id":"T15456","span":{"begin":688,"end":698},"obj":"NNP"},{"id":"T15457","span":{"begin":698,"end":700},"obj":","},{"id":"T15458","span":{"begin":700,"end":706},"obj":"NNP"},{"id":"T15459","span":{"begin":707,"end":713},"obj":"NNP"},{"id":"T15460","span":{"begin":713,"end":714},"obj":"-RRB-"},{"id":"T15461","span":{"begin":714,"end":716},"obj":","},{"id":"T15462","span":{"begin":716,"end":728},"obj":"VBN"},{"id":"T15463","span":{"begin":729,"end":733},"obj":"IN"},{"id":"T15464","span":{"begin":734,"end":736},"obj":"CD"},{"id":"T15465","span":{"begin":736,"end":737},"obj":"NN"},{"id":"T15466","span":{"begin":751,"end":756},"obj":"NN"},{"id":"T15467","span":{"begin":738,"end":743},"obj":"JJ"},{"id":"T15468","span":{"begin":744,"end":750},"obj":"JJ"},{"id":"T15469","span":{"begin":756,"end":758},"obj":","},{"id":"T15470","span":{"begin":758,"end":761},"obj":"CD"},{"id":"T15471","span":{"begin":762,"end":764},"obj":"NN"},{"id":"T15472","span":{"begin":770,"end":779},"obj":"NN"},{"id":"T15473","span":{"begin":764,"end":765},"obj":"SYM"},{"id":"T15474","span":{"begin":765,"end":767},"obj":"NN"},{"id":"T15475","span":{"begin":768,"end":769},"obj":"NN"},{"id":"T15476","span":{"begin":769,"end":770},"obj":"HYPH"},{"id":"T15477","span":{"begin":779,"end":781},"obj":","},{"id":"T15478","span":{"begin":781,"end":784},"obj":"CD"},{"id":"T15479","span":{"begin":785,"end":790},"obj":"NNS"},{"id":"T15480","span":{"begin":794,"end":804},"obj":"NN"},{"id":"T15481","span":{"begin":790,"end":791},"obj":"SYM"},{"id":"T15482","span":{"begin":791,"end":793},"obj":"NN"},{"id":"T15483","span":{"begin":804,"end":806},"obj":","},{"id":"T15484","span":{"begin":806,"end":809},"obj":"CD"},{"id":"T15485","span":{"begin":810,"end":813},"obj":"NN"},{"id":"T15486","span":{"begin":817,"end":829},"obj":"NN"},{"id":"T15487","span":{"begin":813,"end":814},"obj":"SYM"},{"id":"T15488","span":{"begin":814,"end":816},"obj":"NN"},{"id":"T15489","span":{"begin":829,"end":831},"obj":","},{"id":"T15490","span":{"begin":831,"end":834},"obj":"CC"},{"id":"T15491","span":{"begin":835,"end":839},"obj":"CD"},{"id":"T15492","span":{"begin":840,"end":843},"obj":"NN"},{"id":"T15493","span":{"begin":860,"end":861},"obj":"NN"},{"id":"T15494","span":{"begin":843,"end":844},"obj":"SYM"},{"id":"T15495","span":{"begin":844,"end":846},"obj":"NN"},{"id":"T15496","span":{"begin":847,"end":859},"obj":"NN"},{"id":"T15497","span":{"begin":861,"end":862},"obj":"."},{"id":"T15498","span":{"begin":862,"end":932},"obj":"sentence"},{"id":"T15499","span":{"begin":863,"end":867},"obj":"NN"},{"id":"T15500","span":{"begin":868,"end":876},"obj":"NNS"},{"id":"T15501","span":{"begin":882,"end":890},"obj":"VBN"},{"id":"T15502","span":{"begin":877,"end":881},"obj":"VBD"},{"id":"T15503","span":{"begin":891,"end":893},"obj":"IN"},{"id":"T15504","span":{"begin":894,"end":896},"obj":"CD"},{"id":"T15505","span":{"begin":897,"end":899},"obj":"NN"},{"id":"T15506","span":{"begin":900,"end":902},"obj":"IN"},{"id":"T15507","span":{"begin":903,"end":905},"obj":"CD"},{"id":"T15508","span":{"begin":905,"end":906},"obj":"NN"},{"id":"T15509","span":{"begin":907,"end":910},"obj":"NN"},{"id":"T15510","span":{"begin":910,"end":911},"obj":"HYPH"},{"id":"T15511","span":{"begin":911,"end":912},"obj":"CD"},{"id":"T15512","span":{"begin":912,"end":913},"obj":"NN"},{"id":"T15513","span":{"begin":914,"end":917},"obj":"NN"},{"id":"T15514","span":{"begin":918,"end":921},"obj":"IN"},{"id":"T15515","span":{"begin":922,"end":924},"obj":"RB"},{"id":"T15516","span":{"begin":928,"end":929},"obj":"CD"},{"id":"T15517","span":{"begin":925,"end":927},"obj":"IN"},{"id":"T15518","span":{"begin":930,"end":931},"obj":"NN"},{"id":"T15519","span":{"begin":931,"end":932},"obj":"."},{"id":"T15520","span":{"begin":932,"end":1068},"obj":"sentence"},{"id":"T15521","span":{"begin":933,"end":937},"obj":"PRP"},{"id":"T15522","span":{"begin":943,"end":955},"obj":"VBN"},{"id":"T15523","span":{"begin":938,"end":942},"obj":"VBD"},{"id":"T15524","span":{"begin":956,"end":961},"obj":"RB"},{"id":"T15525","span":{"begin":962,"end":966},"obj":"IN"},{"id":"T15526","span":{"begin":967,"end":968},"obj":"DT"},{"id":"T15527","span":{"begin":980,"end":990},"obj":"NN"},{"id":"T15528","span":{"begin":969,"end":979},"obj":"VBG"},{"id":"T15529","span":{"begin":991,"end":992},"obj":"-LRB-"},{"id":"T15530","span":{"begin":992,"end":998},"obj":"NN"},{"id":"T15531","span":{"begin":998,"end":1000},"obj":","},{"id":"T15532","span":{"begin":1000,"end":1005},"obj":"NNP"},{"id":"T15533","span":{"begin":1005,"end":1007},"obj":","},{"id":"T15534","span":{"begin":1007,"end":1014},"obj":"NNP"},{"id":"T15535","span":{"begin":1014,"end":1016},"obj":","},{"id":"T15536","span":{"begin":1016,"end":1023},"obj":"NNP"},{"id":"T15537","span":{"begin":1023,"end":1024},"obj":"-RRB-"},{"id":"T15538","span":{"begin":1025,"end":1033},"obj":"VBN"},{"id":"T15539","span":{"begin":1034,"end":1038},"obj":"IN"},{"id":"T15540","span":{"begin":1039,"end":1040},"obj":"DT"},{"id":"T15541","span":{"begin":1061,"end":1067},"obj":"NN"},{"id":"T15542","span":{"begin":1041,"end":1046},"obj":"NNP"},{"id":"T15543","span":{"begin":1047,"end":1052},"obj":"NN"},{"id":"T15544","span":{"begin":1053,"end":1060},"obj":"JJ"},{"id":"T15545","span":{"begin":1067,"end":1068},"obj":"."}],"relations":[{"id":"R4209","pred":"amod","subj":"T15319","obj":"T15320"},{"id":"R4210","pred":"nsubjpass","subj":"T15320","obj":"T15321"},{"id":"R4211","pred":"auxpass","subj":"T15322","obj":"T15321"},{"id":"R4212","pred":"prt","subj":"T15323","obj":"T15321"},{"id":"R4213","pred":"prep","subj":"T15324","obj":"T15321"},{"id":"R4214","pred":"pobj","subj":"T15325","obj":"T15324"},{"id":"R4215","pred":"prep","subj":"T15326","obj":"T15325"},{"id":"R4216","pred":"nmod","subj":"T15327","obj":"T15328"},{"id":"R4217","pred":"pobj","subj":"T15328","obj":"T15326"},{"id":"R4218","pred":"punct","subj":"T15329","obj":"T15330"},{"id":"R4219","pred":"prep","subj":"T15330","obj":"T15327"},{"id":"R4220","pred":"punct","subj":"T15331","obj":"T15321"},{"id":"R4221","pred":"nsubj","subj":"T15333","obj":"T15334"},{"id":"R4222","pred":"aux","subj":"T15335","obj":"T15334"},{"id":"R4223","pred":"punct","subj":"T15336","obj":"T15334"},{"id":"R4224","pred":"conj","subj":"T15337","obj":"T15334"},{"id":"R4225","pred":"punct","subj":"T15338","obj":"T15337"},{"id":"R4226","pred":"cc","subj":"T15339","obj":"T15337"},{"id":"R4227","pred":"conj","subj":"T15340","obj":"T15337"},{"id":"R4228","pred":"prep","subj":"T15341","obj":"T15340"},{"id":"R4229","pred":"pobj","subj":"T15342","obj":"T15341"},{"id":"R4230","pred":"amod","subj":"T15343","obj":"T15344"},{"id":"R4231","pred":"prep","subj":"T15344","obj":"T15334"},{"id":"R4232","pred":"pobj","subj":"T15345","obj":"T15344"},{"id":"R4233","pred":"punct","subj":"T15346","obj":"T15334"},{"id":"R4234","pred":"prep","subj":"T15348","obj":"T15349"},{"id":"R4235","pred":"amod","subj":"T15350","obj":"T15351"},{"id":"R4236","pred":"pobj","subj":"T15351","obj":"T15348"},{"id":"R4237","pred":"punct","subj":"T15352","obj":"T15349"},{"id":"R4238","pred":"det","subj":"T15353","obj":"T15354"},{"id":"R4239","pred":"nsubjpass","subj":"T15354","obj":"T15349"},{"id":"R4240","pred":"amod","subj":"T15355","obj":"T15354"},{"id":"R4241","pred":"auxpass","subj":"T15356","obj":"T15349"},{"id":"R4242","pred":"prep","subj":"T15357","obj":"T15349"},{"id":"R4243","pred":"amod","subj":"T15358","obj":"T15359"},{"id":"R4244","pred":"pobj","subj":"T15359","obj":"T15357"},{"id":"R4245","pred":"punct","subj":"T15360","obj":"T15349"},{"id":"R4246","pred":"cc","subj":"T15361","obj":"T15349"},{"id":"R4247","pred":"nsubjpass","subj":"T15362","obj":"T15363"},{"id":"R4248","pred":"conj","subj":"T15363","obj":"T15349"},{"id":"R4249","pred":"punct","subj":"T15364","obj":"T15362"},{"id":"R4250","pred":"conj","subj":"T15365","obj":"T15362"},{"id":"R4251","pred":"punct","subj":"T15366","obj":"T15365"},{"id":"R4252","pred":"cc","subj":"T15367","obj":"T15365"},{"id":"R4253","pred":"conj","subj":"T15368","obj":"T15365"},{"id":"R4254","pred":"auxpass","subj":"T15369","obj":"T15363"},{"id":"R4255","pred":"punct","subj":"T15370","obj":"T15349"},{"id":"R4256","pred":"det","subj":"T15372","obj":"T15373"},{"id":"R4257","pred":"nsubjpass","subj":"T15373","obj":"T15374"},{"id":"R4258","pred":"cc","subj":"T15375","obj":"T15373"},{"id":"R4259","pred":"amod","subj":"T15376","obj":"T15377"},{"id":"R4260","pred":"conj","subj":"T15377","obj":"T15373"},{"id":"R4261","pred":"auxpass","subj":"T15378","obj":"T15374"},{"id":"R4262","pred":"cc","subj":"T15379","obj":"T15374"},{"id":"R4263","pred":"conj","subj":"T15380","obj":"T15374"},{"id":"R4264","pred":"punct","subj":"T15381","obj":"T15374"},{"id":"R4265","pred":"amod","subj":"T15383","obj":"T15384"},{"id":"R4266","pred":"nsubjpass","subj":"T15384","obj":"T15385"},{"id":"R4267","pred":"auxpass","subj":"T15386","obj":"T15385"},{"id":"R4268","pred":"prep","subj":"T15387","obj":"T15385"},{"id":"R4269","pred":"amod","subj":"T15388","obj":"T15389"},{"id":"R4270","pred":"pobj","subj":"T15389","obj":"T15387"},{"id":"R4271","pred":"amod","subj":"T15390","obj":"T15389"},{"id":"R4272","pred":"prep","subj":"T15391","obj":"T15389"},{"id":"R4273","pred":"nmod","subj":"T15392","obj":"T15393"},{"id":"R4274","pred":"pobj","subj":"T15393","obj":"T15391"},{"id":"R4275","pred":"cc","subj":"T15394","obj":"T15392"},{"id":"R4276","pred":"conj","subj":"T15395","obj":"T15392"},{"id":"R4277","pred":"punct","subj":"T15396","obj":"T15385"},{"id":"R4278","pred":"prep","subj":"T15398","obj":"T15399"},{"id":"R4279","pred":"compound","subj":"T15400","obj":"T15401"},{"id":"R4280","pred":"pobj","subj":"T15401","obj":"T15398"},{"id":"R4281","pred":"compound","subj":"T15402","obj":"T15401"},{"id":"R4282","pred":"punct","subj":"T15403","obj":"T15399"},{"id":"R4283","pred":"nsubjpass","subj":"T15404","obj":"T15399"},{"id":"R4284","pred":"auxpass","subj":"T15405","obj":"T15399"},{"id":"R4285","pred":"prep","subj":"T15406","obj":"T15399"},{"id":"R4286","pred":"amod","subj":"T15407","obj":"T15408"},{"id":"R4287","pred":"pobj","subj":"T15408","obj":"T15406"},{"id":"R4288","pred":"prep","subj":"T15409","obj":"T15399"},{"id":"R4289","pred":"pobj","subj":"T15410","obj":"T15409"},{"id":"R4290","pred":"cc","subj":"T15411","obj":"T15410"},{"id":"R4291","pred":"conj","subj":"T15412","obj":"T15410"},{"id":"R4292","pred":"cc","subj":"T15413","obj":"T15399"},{"id":"R4293","pred":"conj","subj":"T15414","obj":"T15399"},{"id":"R4294","pred":"prep","subj":"T15415","obj":"T15414"},{"id":"R4295","pred":"amod","subj":"T15416","obj":"T15417"},{"id":"R4296","pred":"pobj","subj":"T15417","obj":"T15415"},{"id":"R4297","pred":"nummod","subj":"T15418","obj":"T15419"},{"id":"R4298","pred":"nmod","subj":"T15419","obj":"T15417"},{"id":"R4299","pred":"punct","subj":"T15420","obj":"T15419"},{"id":"R4300","pred":"punct","subj":"T15421","obj":"T15422"},{"id":"R4301","pred":"parataxis","subj":"T15422","obj":"T15417"},{"id":"R4302","pred":"nummod","subj":"T15423","obj":"T15422"},{"id":"R4303","pred":"punct","subj":"T15424","obj":"T15422"},{"id":"R4304","pred":"punct","subj":"T15425","obj":"T15422"},{"id":"R4305","pred":"compound","subj":"T15426","obj":"T15417"},{"id":"R4306","pred":"acl","subj":"T15427","obj":"T15417"},{"id":"R4307","pred":"prep","subj":"T15428","obj":"T15427"},{"id":"R4308","pred":"pobj","subj":"T15429","obj":"T15428"},{"id":"R4309","pred":"acl","subj":"T15430","obj":"T15429"},{"id":"R4310","pred":"nummod","subj":"T15431","obj":"T15432"},{"id":"R4311","pred":"dobj","subj":"T15432","obj":"T15430"},{"id":"R4312","pred":"prep","subj":"T15433","obj":"T15432"},{"id":"R4313","pred":"poss","subj":"T15434","obj":"T15435"},{"id":"R4314","pred":"pobj","subj":"T15435","obj":"T15433"},{"id":"R4315","pred":"case","subj":"T15436","obj":"T15434"},{"id":"R4316","pred":"amod","subj":"T15437","obj":"T15435"},{"id":"R4317","pred":"poss","subj":"T15438","obj":"T15435"},{"id":"R4318","pred":"case","subj":"T15439","obj":"T15438"},{"id":"R4319","pred":"punct","subj":"T15440","obj":"T15435"},{"id":"R4320","pred":"compound","subj":"T15441","obj":"T15442"},{"id":"R4321","pred":"compound","subj":"T15442","obj":"T15443"},{"id":"R4322","pred":"appos","subj":"T15443","obj":"T15435"},{"id":"R4323","pred":"punct","subj":"T15444","obj":"T15443"},{"id":"R4324","pred":"nummod","subj":"T15445","obj":"T15443"},{"id":"R4325","pred":"punct","subj":"T15446","obj":"T15447"},{"id":"R4326","pred":"parataxis","subj":"T15447","obj":"T15443"},{"id":"R4327","pred":"nummod","subj":"T15448","obj":"T15449"},{"id":"R4328","pred":"dep","subj":"T15449","obj":"T15447"},{"id":"R4329","pred":"punct","subj":"T15450","obj":"T15449"},{"id":"R4330","pred":"punct","subj":"T15451","obj":"T15447"},{"id":"R4331","pred":"punct","subj":"T15452","obj":"T15447"},{"id":"R4332","pred":"compound","subj":"T15453","obj":"T15454"},{"id":"R4333","pred":"npadvmod","subj":"T15454","obj":"T15447"},{"id":"R4334","pred":"punct","subj":"T15455","obj":"T15447"},{"id":"R4335","pred":"npadvmod","subj":"T15456","obj":"T15447"},{"id":"R4336","pred":"punct","subj":"T15457","obj":"T15447"},{"id":"R4337","pred":"compound","subj":"T15458","obj":"T15459"},{"id":"R4338","pred":"npadvmod","subj":"T15459","obj":"T15447"},{"id":"R4339","pred":"punct","subj":"T15460","obj":"T15447"},{"id":"R4340","pred":"punct","subj":"T15461","obj":"T15435"},{"id":"R4341","pred":"acl","subj":"T15462","obj":"T15435"},{"id":"R4342","pred":"prep","subj":"T15463","obj":"T15462"},{"id":"R4343","pred":"nummod","subj":"T15464","obj":"T15465"},{"id":"R4344","pred":"nmod","subj":"T15465","obj":"T15466"},{"id":"R4345","pred":"pobj","subj":"T15466","obj":"T15463"},{"id":"R4346","pred":"amod","subj":"T15467","obj":"T15466"},{"id":"R4347","pred":"amod","subj":"T15468","obj":"T15466"},{"id":"R4348","pred":"punct","subj":"T15469","obj":"T15466"},{"id":"R4349","pred":"nummod","subj":"T15470","obj":"T15471"},{"id":"R4350","pred":"nmod","subj":"T15471","obj":"T15472"},{"id":"R4351","pred":"conj","subj":"T15472","obj":"T15466"},{"id":"R4352","pred":"punct","subj":"T15473","obj":"T15474"},{"id":"R4353","pred":"prep","subj":"T15474","obj":"T15471"},{"id":"R4354","pred":"compound","subj":"T15475","obj":"T15472"},{"id":"R4355","pred":"punct","subj":"T15476","obj":"T15472"},{"id":"R4356","pred":"punct","subj":"T15477","obj":"T15472"},{"id":"R4357","pred":"nummod","subj":"T15478","obj":"T15479"},{"id":"R4358","pred":"nmod","subj":"T15479","obj":"T15480"},{"id":"R4359","pred":"conj","subj":"T15480","obj":"T15472"},{"id":"R4360","pred":"punct","subj":"T15481","obj":"T15482"},{"id":"R4361","pred":"prep","subj":"T15482","obj":"T15479"},{"id":"R4362","pred":"punct","subj":"T15483","obj":"T15480"},{"id":"R4363","pred":"nummod","subj":"T15484","obj":"T15485"},{"id":"R4364","pred":"nmod","subj":"T15485","obj":"T15486"},{"id":"R4365","pred":"conj","subj":"T15486","obj":"T15480"},{"id":"R4366","pred":"punct","subj":"T15487","obj":"T15488"},{"id":"R4367","pred":"prep","subj":"T15488","obj":"T15485"},{"id":"R4368","pred":"punct","subj":"T15489","obj":"T15486"},{"id":"R4369","pred":"cc","subj":"T15490","obj":"T15486"},{"id":"R4370","pred":"nummod","subj":"T15491","obj":"T15492"},{"id":"R4371","pred":"nmod","subj":"T15492","obj":"T15493"},{"id":"R4372","pred":"conj","subj":"T15493","obj":"T15486"},{"id":"R4373","pred":"punct","subj":"T15494","obj":"T15495"},{"id":"R4374","pred":"prep","subj":"T15495","obj":"T15492"},{"id":"R4375","pred":"compound","subj":"T15496","obj":"T15493"},{"id":"R4376","pred":"punct","subj":"T15497","obj":"T15399"},{"id":"R4377","pred":"compound","subj":"T15499","obj":"T15500"},{"id":"R4378","pred":"nsubjpass","subj":"T15500","obj":"T15501"},{"id":"R4379","pred":"auxpass","subj":"T15502","obj":"T15501"},{"id":"R4380","pred":"prep","subj":"T15503","obj":"T15501"},{"id":"R4381","pred":"nummod","subj":"T15504","obj":"T15505"},{"id":"R4382","pred":"pobj","subj":"T15505","obj":"T15503"},{"id":"R4383","pred":"prep","subj":"T15506","obj":"T15501"},{"id":"R4384","pred":"nummod","subj":"T15507","obj":"T15508"},{"id":"R4385","pred":"compound","subj":"T15508","obj":"T15509"},{"id":"R4386","pred":"pobj","subj":"T15509","obj":"T15506"},{"id":"R4387","pred":"punct","subj":"T15510","obj":"T15509"},{"id":"R4388","pred":"nummod","subj":"T15511","obj":"T15512"},{"id":"R4389","pred":"compound","subj":"T15512","obj":"T15513"},{"id":"R4390","pred":"appos","subj":"T15513","obj":"T15509"},{"id":"R4391","pred":"prep","subj":"T15514","obj":"T15501"},{"id":"R4392","pred":"advmod","subj":"T15515","obj":"T15516"},{"id":"R4393","pred":"nummod","subj":"T15516","obj":"T15518"},{"id":"R4394","pred":"quantmod","subj":"T15517","obj":"T15516"},{"id":"R4395","pred":"pobj","subj":"T15518","obj":"T15514"},{"id":"R4396","pred":"punct","subj":"T15519","obj":"T15501"},{"id":"R4397","pred":"nsubjpass","subj":"T15521","obj":"T15522"},{"id":"R4398","pred":"auxpass","subj":"T15523","obj":"T15522"},{"id":"R4399","pred":"advmod","subj":"T15524","obj":"T15522"},{"id":"R4400","pred":"prep","subj":"T15525","obj":"T15522"},{"id":"R4401","pred":"det","subj":"T15526","obj":"T15527"},{"id":"R4402","pred":"pobj","subj":"T15527","obj":"T15525"},{"id":"R4403","pred":"amod","subj":"T15528","obj":"T15527"},{"id":"R4404","pred":"punct","subj":"T15529","obj":"T15530"},{"id":"R4405","pred":"parataxis","subj":"T15530","obj":"T15527"},{"id":"R4406","pred":"punct","subj":"T15531","obj":"T15530"},{"id":"R4407","pred":"npadvmod","subj":"T15532","obj":"T15530"},{"id":"R4408","pred":"punct","subj":"T15533","obj":"T15530"},{"id":"R4409","pred":"npadvmod","subj":"T15534","obj":"T15530"},{"id":"R4410","pred":"punct","subj":"T15535","obj":"T15530"},{"id":"R4411","pred":"npadvmod","subj":"T15536","obj":"T15530"},{"id":"R4412","pred":"punct","subj":"T15537","obj":"T15530"},{"id":"R4413","pred":"acl","subj":"T15538","obj":"T15527"},{"id":"R4414","pred":"prep","subj":"T15539","obj":"T15538"},{"id":"R4415","pred":"det","subj":"T15540","obj":"T15541"},{"id":"R4416","pred":"pobj","subj":"T15541","obj":"T15539"},{"id":"R4417","pred":"nmod","subj":"T15542","obj":"T15543"},{"id":"R4418","pred":"nmod","subj":"T15543","obj":"T15541"},{"id":"R4419","pred":"amod","subj":"T15544","obj":"T15541"},{"id":"R4420","pred":"punct","subj":"T15545","obj":"T15522"}],"text":"Timed pregnancies were set up for collection of E11.5–E17.5 embryos. Embryos were fixed, dehydrated, and embedded in paraffin prior to sectioning. In older embryos, a median sternotomy was performed under microscopic guidance, and diaphragm, lungs, and heart were examined. The lungs and tracheobronchial tree were removed and weighed. Whole diaphragms were isolated from fixed thoracic tissue from E15.5 and E17.5 embryos. For lung explant culture, lungs were dissected from fresh embryos at E11.5 and E12.5 and placed on porous 24-mm (0.4-μ) polyester membranes floated in wells containing 2 ml of Dulbecco's modified Eagle's medium, nutrient mixture F-12 (11039–021, Gibco, San Diego, California, United States), supplemented with 10% fetal bovine serum, 0.3 mg/ml L-glutamine, 100 units/ml penicillin, 100 mcg/ml streptomycin, and 0.25 mcg/ml amphotericin B. Lung explants were cultured at 37 °C in 95% air/5% CO2 for up to 5 d. They were photographed daily with a dissecting microscope (MZ12.5, Leica, Wetzlar, Germany) equipped with a Leica DC500 digital camera."}