PMC:1181811 / 30171-30464
Annnotations
craft-sa-dev
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Entire coding regions plus ~50 kb of flanking sequence of each single-exon Tas2r was amplified from D2 or BXD RI genomic DNA (Jackson Laboratories, Bar Harbor, ME) by polymerase chain reaction (PCR) using a high-fidelity polymerase TaqPro Complete (Denville Scientific, South Plainfield, NJ)."}
craft-ca-core-ex-dev
{"project":"craft-ca-core-ex-dev","denotations":[{"id":"T6107","span":{"begin":222,"end":232},"obj":"GO_EXT:polymerase"},{"id":"T6106","span":{"begin":168,"end":178},"obj":"GO_EXT:polymerase"},{"id":"T6105","span":{"begin":122,"end":125},"obj":"CHEBI_SO_EXT:DNA"},{"id":"T6104","span":{"begin":114,"end":125},"obj":"SO_EXT:genomic_DNA"},{"id":"T6103","span":{"begin":76,"end":81},"obj":"PR_EXT:000036337"},{"id":"T6102","span":{"begin":71,"end":75},"obj":"SO_EXT:0000147"},{"id":"T6101","span":{"begin":47,"end":55},"obj":"SO_EXT:biological_sequence"},{"id":"T6100","span":{"begin":38,"end":46},"obj":"SO:0000357"},{"id":"T6099","span":{"begin":33,"end":34},"obj":"CHEBI_SO_EXT:base"},{"id":"T6098","span":{"begin":8,"end":22},"obj":"SO_EXT:coding_sequence"}],"text":" Entire coding regions plus ~50 kb of flanking sequence of each single-exon Tas2r was amplified from D2 or BXD RI genomic DNA (Jackson Laboratories, Bar Harbor, ME) by polymerase chain reaction (PCR) using a high-fidelity polymerase TaqPro Complete (Denville Scientific, South Plainfield, NJ)."}
craft-ca-core-dev
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