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tests\nAll behavioral tests were conducted in the commercially available Davis MS-160 gustometer (DiLog Instruments, Inc., Tallahassee FL). Testing procedures were similar to those described earlier [29,37]. Briefly, after 24 hours of water deprivation, naïve mice are given a single 20-minute trial consisting of access to a single bottle of distilled water (sipper tube training). On day 2, mice could initiate up to sixteen 5 s trials with a single lick to one of four bottles containing distilled water (trial training). Testing occurred in sessions 3 and 4, with one test session per day per mouse. Trials were 5 s in length with an inter-trial interval of 10 s, and mice had up to 120 s to initiate a trial; if a trial was not initiated during this interval, the shutter closed for 10 s and the next trial was presented. Mice were tested with 2 concentrations each of 4 different taste stimuli [1 and 3 mM QHCl, 1 and 3 mM DB, 3 and 10 mM PROP (unpublished data), and 0.01 and 0.1 M sucrose]. Stimulus trials were presented in 3 blocks of 8 trials, for a total of 24 possible trials per test session. Each block consisted of each concentration of stimulus plus four presentations of distilled water in random order. Individual mice were also tested in random order.\nThe dependent measure for each computed for each mouse was the lick ratio (average number of licks to stimulusx /average number of licks to water) where x is a given concentration of stimulus and the average number of licks to water is derived from the water trials during both test sessions. Lick ratio data for each stimulus were compiled for all individual mice, and means were prepared for each strain. B6 vs. D2 comparisons (Fig. 1A) were made using main effects ANOVA. Lick ratios for individual mice to sucrose were generally ~1.0 (data not shown), indicating that either concentration of this compound was licked at a similar rate to water by these thirsty animals. This stimulus was intended as a \"neutral\" stimulus, albeit one that has different sensory properties than water, and therefore not analyzed further. This was done to encourage sampling on \"non-water\" trials, as there is some evidence that mice detect distilled vs. adulterated water in brief-access taste tests based on olfactory clues; there is no evidence that mice can detect or distinguish among concentrations of a particular stimulus [37]."}

{"project":"craft-ca-core-ex-dev","denotations":[{"id":"T5392","span":{"begin":2321,"end":2325},"obj":"NCBITaxon:10088"},{"id":"T5391","span":{"begin":2278,"end":2287},"obj":"GO:0007608"},{"id":"T5390","span":{"begin":2257,"end":2262},"obj":"GO:0050909"},{"id":"T5389","span":{"begin":2235,"end":2240},"obj":"CHEBI:15377"},{"id":"T5388","span":{"begin":2197,"end":2201},"obj":"NCBITaxon:10088"},{"id":"T5387","span":{"begin":2151,"end":2156},"obj":"CHEBI:15377"},{"id":"T5386","span":{"begin":2064,"end":2069},"obj":"CHEBI:15377"},{"id":"T5385","span":{"begin":2040,"end":2047},"obj":"GO_EXT:detection_or_sensing_of_stimulus"},{"id":"T5384","span":{"begin":1949,"end":1956},"obj":"NCBITaxon:33208"},{"id":"T5383","span":{"begin":1926,"end":1931},"obj":"CHEBI:15377"},{"id":"T5382","span":{"begin":1885,"end":1893},"obj":"CHEBI:36357"},{"id":"T5381","span":{"begin":1794,"end":1801},"obj":"CHEBI:17992"},{"id":"T5380","span":{"begin":1786,"end":1790},"obj":"NCBITaxon:10088"},{"id":"T5379","span":{"begin":1644,"end":1648},"obj":"NCBITaxon:10088"},{"id":"T5378","span":{"begin":1537,"end":1542},"obj":"CHEBI:15377"},{"id":"T5377","span":{"begin":1511,"end":1516},"obj":"CHEBI:15377"},{"id":"T5376","span":{"begin":1424,"end":1429},"obj":"CHEBI:15377"},{"id":"T5375","span":{"begin":1333,"end":1338},"obj":"NCBITaxon:10088"},{"id":"T5374","span":{"begin":1245,"end":1249},"obj":"NCBITaxon:10088"},{"id":"T5373","span":{"begin":1211,"end":1216},"obj":"CHEBI:15377"},{"id":"T5372","span":{"begin":1001,"end":1008},"obj":"CHEBI:17992"},{"id":"T5371","span":{"begin":957,"end":961},"obj":"CHEBI:8502"},{"id":"T5370","span":{"begin":898,"end":903},"obj":"GO:0050909"},{"id":"T5369","span":{"begin":839,"end":843},"obj":"NCBITaxon:10088"},{"id":"T5368","span":{"begin":684,"end":688},"obj":"NCBITaxon:10088"},{"id":"T5367","span":{"begin":609,"end":614},"obj":"NCBITaxon:10088"},{"id":"T5366","span":{"begin":513,"end":518},"obj":"CHEBI:15377"},{"id":"T5365","span":{"begin":405,"end":409},"obj":"NCBITaxon:10088"},{"id":"T5364","span":{"begin":365,"end":370},"obj":"CHEBI:15377"},{"id":"T5363","span":{"begin":272,"end":276},"obj":"NCBITaxon:10088"},{"id":"T5362","span":{"begin":247,"end":252},"obj":"CHEBI:15377"},{"id":"T5361","span":{"begin":23,"end":33},"obj":"GO_PATO_EXT:biological_behavior"}],"text":"Brief-access tests\nAll behavioral tests were conducted in the commercially available Davis MS-160 gustometer (DiLog Instruments, Inc., Tallahassee FL). Testing procedures were similar to those described earlier [29,37]. Briefly, after 24 hours of water deprivation, naïve mice are given a single 20-minute trial consisting of access to a single bottle of distilled water (sipper tube training). On day 2, mice could initiate up to sixteen 5 s trials with a single lick to one of four bottles containing distilled water (trial training). Testing occurred in sessions 3 and 4, with one test session per day per mouse. Trials were 5 s in length with an inter-trial interval of 10 s, and mice had up to 120 s to initiate a trial; if a trial was not initiated during this interval, the shutter closed for 10 s and the next trial was presented. Mice were tested with 2 concentrations each of 4 different taste stimuli [1 and 3 mM QHCl, 1 and 3 mM DB, 3 and 10 mM PROP (unpublished data), and 0.01 and 0.1 M sucrose]. Stimulus trials were presented in 3 blocks of 8 trials, for a total of 24 possible trials per test session. Each block consisted of each concentration of stimulus plus four presentations of distilled water in random order. Individual mice were also tested in random order.\nThe dependent measure for each computed for each mouse was the lick ratio (average number of licks to stimulusx /average number of licks to water) where x is a given concentration of stimulus and the average number of licks to water is derived from the water trials during both test sessions. Lick ratio data for each stimulus were compiled for all individual mice, and means were prepared for each strain. B6 vs. D2 comparisons (Fig. 1A) were made using main effects ANOVA. Lick ratios for individual mice to sucrose were generally ~1.0 (data not shown), indicating that either concentration of this compound was licked at a similar rate to water by these thirsty animals. This stimulus was intended as a \"neutral\" stimulus, albeit one that has different sensory properties than water, and therefore not analyzed further. This was done to encourage sampling on \"non-water\" trials, as there is some evidence that mice detect distilled vs. adulterated water in brief-access taste tests based on olfactory clues; there is no evidence that mice can detect or distinguish among concentrations of a particular stimulus [37]."}

{"project":"craft-ca-core-dev","denotations":[{"id":"T5360","span":{"begin":2321,"end":2325},"obj":"NCBITaxon:10088"},{"id":"T5359","span":{"begin":2278,"end":2287},"obj":"GO:0007608"},{"id":"T5358","span":{"begin":2257,"end":2262},"obj":"GO:0050909"},{"id":"T5357","span":{"begin":2235,"end":2240},"obj":"CHEBI:15377"},{"id":"T5356","span":{"begin":2197,"end":2201},"obj":"NCBITaxon:10088"},{"id":"T5355","span":{"begin":2151,"end":2156},"obj":"CHEBI:15377"},{"id":"T5354","span":{"begin":2064,"end":2069},"obj":"CHEBI:15377"},{"id":"T5353","span":{"begin":1949,"end":1956},"obj":"NCBITaxon:33208"},{"id":"T5352","span":{"begin":1926,"end":1931},"obj":"CHEBI:15377"},{"id":"T5351","span":{"begin":1885,"end":1893},"obj":"CHEBI:36357"},{"id":"T5350","span":{"begin":1794,"end":1801},"obj":"CHEBI:17992"},{"id":"T5349","span":{"begin":1786,"end":1790},"obj":"NCBITaxon:10088"},{"id":"T5348","span":{"begin":1644,"end":1648},"obj":"NCBITaxon:10088"},{"id":"T5347","span":{"begin":1537,"end":1542},"obj":"CHEBI:15377"},{"id":"T5346","span":{"begin":1511,"end":1516},"obj":"CHEBI:15377"},{"id":"T5345","span":{"begin":1424,"end":1429},"obj":"CHEBI:15377"},{"id":"T5344","span":{"begin":1333,"end":1338},"obj":"NCBITaxon:10088"},{"id":"T5343","span":{"begin":1245,"end":1249},"obj":"NCBITaxon:10088"},{"id":"T5342","span":{"begin":1211,"end":1216},"obj":"CHEBI:15377"},{"id":"T5341","span":{"begin":1001,"end":1008},"obj":"CHEBI:17992"},{"id":"T5340","span":{"begin":957,"end":961},"obj":"CHEBI:8502"},{"id":"T5339","span":{"begin":898,"end":903},"obj":"GO:0050909"},{"id":"T5338","span":{"begin":839,"end":843},"obj":"NCBITaxon:10088"},{"id":"T5337","span":{"begin":684,"end":688},"obj":"NCBITaxon:10088"},{"id":"T5336","span":{"begin":609,"end":614},"obj":"NCBITaxon:10088"},{"id":"T5335","span":{"begin":513,"end":518},"obj":"CHEBI:15377"},{"id":"T5334","span":{"begin":405,"end":409},"obj":"NCBITaxon:10088"},{"id":"T5333","span":{"begin":365,"end":370},"obj":"CHEBI:15377"},{"id":"T5332","span":{"begin":272,"end":276},"obj":"NCBITaxon:10088"},{"id":"T5331","span":{"begin":247,"end":252},"obj":"CHEBI:15377"}],"text":"Brief-access tests\nAll behavioral tests were conducted in the commercially available Davis MS-160 gustometer (DiLog Instruments, Inc., Tallahassee FL). Testing procedures were similar to those described earlier [29,37]. Briefly, after 24 hours of water deprivation, naïve mice are given a single 20-minute trial consisting of access to a single bottle of distilled water (sipper tube training). On day 2, mice could initiate up to sixteen 5 s trials with a single lick to one of four bottles containing distilled water (trial training). Testing occurred in sessions 3 and 4, with one test session per day per mouse. Trials were 5 s in length with an inter-trial interval of 10 s, and mice had up to 120 s to initiate a trial; if a trial was not initiated during this interval, the shutter closed for 10 s and the next trial was presented. Mice were tested with 2 concentrations each of 4 different taste stimuli [1 and 3 mM QHCl, 1 and 3 mM DB, 3 and 10 mM PROP (unpublished data), and 0.01 and 0.1 M sucrose]. Stimulus trials were presented in 3 blocks of 8 trials, for a total of 24 possible trials per test session. Each block consisted of each concentration of stimulus plus four presentations of distilled water in random order. Individual mice were also tested in random order.\nThe dependent measure for each computed for each mouse was the lick ratio (average number of licks to stimulusx /average number of licks to water) where x is a given concentration of stimulus and the average number of licks to water is derived from the water trials during both test sessions. Lick ratio data for each stimulus were compiled for all individual mice, and means were prepared for each strain. B6 vs. D2 comparisons (Fig. 1A) were made using main effects ANOVA. Lick ratios for individual mice to sucrose were generally ~1.0 (data not shown), indicating that either concentration of this compound was licked at a similar rate to water by these thirsty animals. This stimulus was intended as a \"neutral\" stimulus, albeit one that has different sensory properties than water, and therefore not analyzed further. This was done to encourage sampling on \"non-water\" trials, as there is some evidence that mice detect distilled vs. adulterated water in brief-access taste tests based on olfactory clues; there is no evidence that mice can detect or distinguish among concentrations of a particular stimulus [37]."}