PMC:1181811 / 14691-16158 JSONTXT

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A third, Tas2r120, could not be amplified from D2 genomic DNA (Figure 5) using either of two pairs of oligonucleotides (Additional file 2), suggesting that this Tas2r is deleted in D2 mice. Two D2 alleles, Tas2r103 and Tas2r117, contained numerous missense mutations and small deletions that create frame shifts and premature termination; these two genes may be pseudogenes in this strain. The remaining 19 Tas2rs contained between one and 16 missense mutations. All 24 Tas2rs examined have different alleles in B6 and D2 mice, and 307 single nucleotide polymorphisms are present within coding exons (data not shown). 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