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PMC:116589 / 801-1819 JSONTXT

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Id Subject Object Predicate Lexical cue
T292 0-7 NNS denotes Results
T293 7-255 sentence denotes In this study we have used embryonic stem (ES) cell-mediated transgenesis to test the enhanced cyan fluorescent protein (ECFP) and enhanced yellow fluorescent protein (EYFP), two mutant and spectrally distinct color variants of wild type (wt) GFP.
T294 8-10 IN denotes In
T295 30-34 VBN denotes used
T296 11-15 DT denotes this
T297 16-21 NN denotes study
T298 22-24 PRP denotes we
T299 25-29 VBP denotes have
T300 35-44 JJ denotes embryonic
T301 45-49 NN denotes stem
T302 55-59 NN denotes cell
T303 50-51 -LRB- denotes (
T304 51-53 NN denotes ES
T305 53-54 -RRB- denotes )
T306 60-68 VBN denotes mediated
T307 59-60 HYPH denotes -
T308 69-81 NN denotes transgenesis
T309 82-84 TO denotes to
T310 85-89 VB denotes test
T311 90-93 DT denotes the
T312 120-127 NN denotes protein
T313 94-102 VBN denotes enhanced
T314 103-107 NN denotes cyan
T315 108-119 JJ denotes fluorescent
T316 128-129 -LRB- denotes (
T317 129-133 NN denotes ECFP
T318 133-134 -RRB- denotes )
T319 135-138 CC denotes and
T320 139-147 VBN denotes enhanced
T321 167-174 NN denotes protein
T322 148-154 JJ denotes yellow
T323 155-166 JJ denotes fluorescent
T324 175-176 -LRB- denotes (
T325 176-180 NN denotes EYFP
T326 180-181 -RRB- denotes )
T327 181-183 , denotes ,
T328 183-186 CD denotes two
T329 224-232 NNS denotes variants
T330 187-193 JJ denotes mutant
T331 194-197 CC denotes and
T332 198-208 RB denotes spectrally
T333 209-217 JJ denotes distinct
T334 218-223 NN denotes color
T335 233-235 IN denotes of
T336 236-240 JJ denotes wild
T337 241-245 NN denotes type
T338 251-254 NN denotes GFP
T339 246-247 -LRB- denotes (
T340 247-249 NN denotes wt
T341 249-250 -RRB- denotes )
T342 254-255 . denotes .
T343 255-402 sentence denotes We have also tested DsRed1, the novel red fluorescent protein reporter recently cloned from the Discostoma coral by virtue of its homology to GFP.
T344 256-258 PRP denotes We
T345 269-275 VBN denotes tested
T346 259-263 VBP denotes have
T347 264-268 RB denotes also
T348 276-282 NN denotes DsRed1
T349 282-284 , denotes ,
T350 284-287 DT denotes the
T351 318-326 NN denotes reporter
T352 288-293 JJ denotes novel
T353 294-297 JJ denotes red
T354 310-317 NN denotes protein
T355 298-309 JJ denotes fluorescent
T356 327-335 RB denotes recently
T357 336-342 VBN denotes cloned
T358 343-347 IN denotes from
T359 348-351 DT denotes the
T360 363-368 NN denotes coral
T361 352-362 NN denotes Discostoma
T362 369-371 IN denotes by
T363 372-378 NN denotes virtue
T364 379-381 IN denotes of
T365 382-385 PRP$ denotes its
T366 386-394 NN denotes homology
T367 395-397 IN denotes to
T368 398-401 NN denotes GFP
T369 401-402 . denotes .
T370 402-570 sentence denotes To this end, we have established lines of ES cells together with viable and fertile mice having widespread expression of either the ECFP or EYFP GFP-variant reporters.
T371 403-405 IN denotes To
T372 424-435 VBN denotes established
T373 406-410 DT denotes this
T374 411-414 NN denotes end
T375 414-416 , denotes ,
T376 416-418 PRP denotes we
T377 419-423 VBP denotes have
T378 436-441 NNS denotes lines
T379 442-444 IN denotes of
T380 445-447 NN denotes ES
T381 448-453 NNS denotes cells
T382 454-462 RB denotes together
T383 463-467 IN denotes with
T384 468-474 JJ denotes viable
T385 487-491 NNS denotes mice
T386 475-478 CC denotes and
T387 479-486 JJ denotes fertile
T388 492-498 VBG denotes having
T389 499-509 JJ denotes widespread
T390 510-520 NN denotes expression
T391 521-523 IN denotes of
T392 524-530 CC denotes either
T393 560-569 NNS denotes reporters
T394 531-534 DT denotes the
T395 535-539 NN denotes ECFP
T396 540-542 CC denotes or
T397 543-547 NN denotes EYFP
T398 548-551 NN denotes GFP
T399 552-559 NN denotes variant
T400 551-552 HYPH denotes -
T401 569-570 . denotes .
T402 570-750 sentence denotes However, we were unable to generate equivalent DsRed1 lines, suggesting that DsRed1 is not developmentally neutral or that transgene expression cannot be sustained constitutively.
T403 571-578 RB denotes However
T404 583-587 VBD denotes were
T405 578-580 , denotes ,
T406 580-582 PRP denotes we
T407 588-594 JJ denotes unable
T408 595-597 TO denotes to
T409 598-606 VB denotes generate
T410 607-617 JJ denotes equivalent
T411 625-630 NNS denotes lines
T412 618-624 NN denotes DsRed1
T413 630-632 , denotes ,
T414 632-642 VBG denotes suggesting
T415 643-647 IN denotes that
T416 655-657 VBZ denotes is
T417 648-654 NN denotes DsRed1
T418 658-661 RB denotes not
T419 662-677 RB denotes developmentally
T420 678-685 JJ denotes neutral
T421 686-688 CC denotes or
T422 689-693 IN denotes that
T423 725-734 VBN denotes sustained
T424 694-703 NN denotes transgene
T425 704-714 NN denotes expression
T426 715-718 MD denotes can
T427 718-721 RB denotes not
T428 722-724 VB denotes be
T429 735-749 RB denotes constitutively
T430 749-750 . denotes .
T431 750-1018 sentence denotes Balanced (diploid <-> diploid) and polarized (tetraploid <-> diploid) chimeras comprising combinations of the ECFP and EYFP ES cells and/or embryos, demonstrate that populations of cells expressing each individual reporter can be distinguished within a single animal.
T432 751-759 JJ denotes Balanced
T433 821-829 NNS denotes chimeras
T434 760-761 -LRB- denotes (
T435 773-780 JJ denotes diploid
T436 761-768 JJ denotes diploid
T437 769-770 SYM denotes <
T438 770-771 HYPH denotes -
T439 771-772 SYM denotes >
T440 780-781 -RRB- denotes )
T441 782-785 CC denotes and
T442 786-795 JJ denotes polarized
T443 796-797 -LRB- denotes (
T444 812-819 JJ denotes diploid
T445 797-807 JJ denotes tetraploid
T446 808-809 SYM denotes <
T447 809-810 HYPH denotes -
T448 810-811 SYM denotes >
T449 819-820 -RRB- denotes )
T450 900-911 VBP denotes demonstrate
T451 830-840 VBG denotes comprising
T452 841-853 NNS denotes combinations
T453 854-856 IN denotes of
T454 857-860 DT denotes the
T455 861-865 NN denotes ECFP
T456 866-869 CC denotes and
T457 870-874 NN denotes EYFP
T458 875-877 NN denotes ES
T459 878-883 NNS denotes cells
T460 884-887 CC denotes and
T461 887-888 HYPH denotes /
T462 888-890 CC denotes or
T463 891-898 NNS denotes embryos
T464 898-900 , denotes ,
T465 912-916 IN denotes that
T466 981-994 VBN denotes distinguished
T467 917-928 NNS denotes populations
T468 929-931 IN denotes of
T469 932-937 NNS denotes cells
T470 938-948 VBG denotes expressing
T471 949-953 DT denotes each
T472 965-973 NN denotes reporter
T473 954-964 JJ denotes individual
T474 974-977 MD denotes can
T475 978-980 VB denotes be
T476 995-1001 IN denotes within
T477 1002-1003 DT denotes a
T478 1011-1017 NN denotes animal
T479 1004-1010 JJ denotes single
T480 1017-1018 . denotes .
R129 T294 T295 prep In,used
R130 T296 T297 det this,study
R131 T297 T294 pobj study,In
R132 T298 T295 nsubj we,used
R133 T299 T295 aux have,used
R134 T300 T301 amod embryonic,stem
R135 T301 T302 nmod stem,cell
R136 T302 T306 npadvmod cell,mediated
R137 T303 T301 punct (,stem
R138 T304 T301 appos ES,stem
R139 T305 T302 punct ),cell
R140 T306 T308 amod mediated,transgenesis
R141 T307 T306 punct -,mediated
R142 T308 T295 dobj transgenesis,used
R143 T309 T310 aux to,test
R144 T310 T295 advcl test,used
R145 T311 T312 det the,protein
R146 T312 T310 dobj protein,test
R147 T313 T312 amod enhanced,protein
R148 T314 T312 nmod cyan,protein
R149 T315 T312 amod fluorescent,protein
R150 T316 T312 punct (,protein
R151 T317 T312 appos ECFP,protein
R152 T318 T312 punct ),protein
R153 T319 T312 cc and,protein
R154 T320 T321 amod enhanced,protein
R155 T321 T312 conj protein,protein
R156 T322 T321 amod yellow,protein
R157 T323 T321 amod fluorescent,protein
R158 T324 T321 punct (,protein
R159 T325 T321 appos EYFP,protein
R160 T326 T312 punct ),protein
R161 T327 T312 punct ", ",protein
R162 T328 T329 nummod two,variants
R163 T329 T312 appos variants,protein
R164 T330 T329 amod mutant,variants
R165 T331 T330 cc and,mutant
R166 T332 T333 advmod spectrally,distinct
R167 T333 T330 conj distinct,mutant
R168 T334 T329 compound color,variants
R169 T335 T329 prep of,variants
R170 T336 T337 amod wild,type
R171 T337 T338 nmod type,GFP
R172 T338 T335 pobj GFP,of
R173 T339 T337 punct (,type
R174 T340 T337 appos wt,type
R175 T341 T338 punct ),GFP
R176 T342 T295 punct .,used
R177 T344 T345 nsubj We,tested
R178 T346 T345 aux have,tested
R179 T347 T345 advmod also,tested
R180 T348 T345 dobj DsRed1,tested
R181 T349 T348 punct ", ",DsRed1
R182 T350 T351 det the,reporter
R183 T351 T348 appos reporter,DsRed1
R184 T352 T351 amod novel,reporter
R185 T353 T354 amod red,protein
R186 T354 T351 compound protein,reporter
R187 T355 T354 amod fluorescent,protein
R188 T356 T357 advmod recently,cloned
R189 T357 T351 acl cloned,reporter
R190 T358 T357 prep from,cloned
R191 T359 T360 det the,coral
R192 T360 T358 pobj coral,from
R193 T361 T360 compound Discostoma,coral
R194 T362 T357 prep by,cloned
R195 T363 T362 pobj virtue,by
R196 T364 T363 prep of,virtue
R197 T365 T366 poss its,homology
R198 T366 T364 pobj homology,of
R199 T367 T366 prep to,homology
R200 T368 T367 pobj GFP,to
R201 T369 T345 punct .,tested
R202 T371 T372 prep To,established
R203 T373 T374 det this,end
R204 T374 T371 pobj end,To
R205 T375 T372 punct ", ",established
R206 T376 T372 nsubj we,established
R207 T377 T372 aux have,established
R208 T378 T372 dobj lines,established
R209 T379 T378 prep of,lines
R210 T380 T381 compound ES,cells
R211 T381 T379 pobj cells,of
R212 T382 T383 advmod together,with
R213 T383 T372 prep with,established
R214 T384 T385 amod viable,mice
R215 T385 T383 pobj mice,with
R216 T386 T384 cc and,viable
R217 T387 T384 conj fertile,viable
R218 T388 T385 acl having,mice
R219 T389 T390 amod widespread,expression
R220 T390 T388 dobj expression,having
R221 T391 T390 prep of,expression
R222 T392 T393 preconj either,reporters
R224 T394 T393 det the,reporters
R225 T395 T393 nmod ECFP,reporters
R226 T396 T395 cc or,ECFP
R227 T397 T395 conj EYFP,ECFP
R228 T398 T399 compound GFP,variant
R229 T399 T393 compound variant,reporters
R230 T400 T399 punct -,variant
R231 T401 T372 punct .,established
R232 T403 T404 advmod However,were
R233 T405 T404 punct ", ",were
R234 T406 T404 nsubj we,were
R235 T407 T404 acomp unable,were
R236 T408 T409 aux to,generate
R237 T409 T407 xcomp generate,unable
R238 T410 T411 amod equivalent,lines
R239 T411 T409 dobj lines,generate
R240 T412 T411 compound DsRed1,lines
R241 T413 T404 punct ", ",were
R242 T414 T404 advcl suggesting,were
R243 T415 T416 mark that,is
R244 T416 T414 advcl is,suggesting
R245 T417 T416 nsubj DsRed1,is
R246 T418 T416 neg not,is
R247 T419 T420 advmod developmentally,neutral
R248 T420 T416 acomp neutral,is
R249 T421 T416 cc or,is
R250 T422 T423 mark that,sustained
R251 T423 T416 conj sustained,is
R252 T424 T425 compound transgene,expression
R253 T425 T423 nsubjpass expression,sustained
R254 T426 T423 aux can,sustained
R255 T427 T423 neg not,sustained
R256 T428 T423 auxpass be,sustained
R257 T429 T423 advmod constitutively,sustained
R258 T430 T404 punct .,were
R259 T432 T433 amod Balanced,chimeras
R260 T433 T450 nsubj chimeras,demonstrate
R261 T434 T435 punct (,diploid
R262 T435 T432 parataxis diploid,Balanced
R263 T436 T435 nsubj diploid,diploid
R264 T437 T435 punct <,diploid
R265 T438 T435 punct -,diploid
R266 T439 T435 punct >,diploid
R267 T440 T435 punct ),diploid
R268 T441 T432 cc and,Balanced
R269 T442 T432 conj polarized,Balanced
R270 T443 T444 punct (,diploid
R271 T444 T442 parataxis diploid,polarized
R272 T445 T444 nsubj tetraploid,diploid
R273 T446 T444 punct <,diploid
R274 T447 T444 punct -,diploid
R275 T448 T444 punct >,diploid
R276 T449 T444 punct ),diploid
R277 T451 T433 acl comprising,chimeras
R278 T452 T451 dobj combinations,comprising
R279 T453 T452 prep of,combinations
R280 T454 T455 det the,ECFP
R281 T455 T453 pobj ECFP,of
R282 T456 T455 cc and,ECFP
R283 T457 T455 conj EYFP,ECFP
R284 T458 T459 compound ES,cells
R285 T459 T455 appos cells,ECFP
R286 T460 T459 cc and,cells
R287 T461 T460 punct /,and
R288 T462 T460 cc or,and
R289 T463 T459 conj embryos,cells
R290 T464 T450 punct ", ",demonstrate
R291 T465 T466 mark that,distinguished
R292 T466 T450 ccomp distinguished,demonstrate
R293 T467 T466 nsubjpass populations,distinguished
R294 T468 T467 prep of,populations
R295 T469 T468 pobj cells,of
R296 T470 T467 acl expressing,populations
R297 T471 T472 det each,reporter
R298 T472 T470 dobj reporter,expressing
R299 T473 T472 amod individual,reporter
R300 T474 T466 aux can,distinguished
R301 T475 T466 auxpass be,distinguished
R302 T476 T466 prep within,distinguished
R303 T477 T478 det a,animal
R304 T478 T476 pobj animal,within
R305 T479 T478 amod single,animal
R306 T480 T450 punct .,demonstrate
R223 T393 T391 pobj reporters,of

craft-ca-core-ex-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T117 35-44 UBERON:0000922 denotes embryonic
T118 35-49 _FRAGMENT denotes embryonic stem
T119 55-59 CL:0002322 denotes cell
T120 51-53 _FRAGMENT denotes ES
T121 55-59 CL_GO_EXT:cell denotes cell
T122 69-81 SO_EXT:transgenesis denotes transgenesis
T123 120-127 CHEBI_PR_EXT:protein denotes protein
T124 167-174 CHEBI_PR_EXT:protein denotes protein
T125 187-193 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T126 224-232 SO_EXT:sequence_variant_entity denotes variants
T127 236-245 SO_EXT:wild_type_entity_or_quality denotes wild type
T128 247-249 SO_EXT:wild_type_entity_or_quality denotes wt
T129 310-317 CHEBI_PR_EXT:protein denotes protein
T130 336-342 SO_EXT:sequence_cloning_process denotes cloned
T131 352-362 NCBITaxon:86599 denotes Discostoma
T132 386-394 SO:0000857 denotes homology
T133 445-453 CL:0002322 denotes ES cells
T134 448-453 CL_GO_EXT:cell denotes cells
T135 487-491 NCBITaxon:10088 denotes mice
T136 510-520 GO:0010467 denotes expression
T137 552-559 SO_EXT:sequence_variant_or_variation denotes variant
T138 694-703 SO_EXT:0000902 denotes transgene
T139 704-714 GO:0010467 denotes expression
T140 875-883 CL:0002322 denotes ES cells
T141 878-883 CL_GO_EXT:cell denotes cells
T142 891-898 UBERON:0000922 denotes embryos
T143 917-937 UBERON_EXT:cell_clump_or_cluster_or_group_or_mass_or_population denotes populations of cells
T144 932-937 CL_GO_EXT:cell denotes cells
T145 938-948 GO:0010467 denotes expressing
T146 1011-1017 NCBITaxon:33208 denotes animal
R7 T119 T118 _lexicallyChainedTo cell,embryonic stem
R8 T119 T120 _lexicallyChainedTo cell,ES

craft-ca-core-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T71 35-44 UBERON:0000922 denotes embryonic
T72 35-49 _FRAGMENT denotes embryonic stem
T73 55-59 CL:0002322 denotes cell
T74 51-53 _FRAGMENT denotes ES
T75 352-362 NCBITaxon:86599 denotes Discostoma
T76 386-394 SO:0000857 denotes homology
T77 445-453 CL:0002322 denotes ES cells
T78 487-491 NCBITaxon:10088 denotes mice
T79 510-520 GO:0010467 denotes expression
T80 694-703 SO:0000902 denotes transgene
T81 704-714 GO:0010467 denotes expression
T82 875-883 CL:0002322 denotes ES cells
T83 891-898 UBERON:0000922 denotes embryos
T84 938-948 GO:0010467 denotes expressing
T85 1011-1017 NCBITaxon:33208 denotes animal
R3 T73 T72 _lexicallyChainedTo cell,embryonic stem
R4 T73 T74 _lexicallyChainedTo cell,ES