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PMC:116589 / 16467-18504 JSONTXT

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Id Subject Object Predicate Lexical cue
T2695 12-16 IN denotes From
T2696 39-47 VBP denotes conclude
T2697 17-22 DT denotes these
T2698 23-35 NNS denotes observations
T2699 36-38 PRP denotes we
T2700 48-52 IN denotes that
T2701 119-122 VBP denotes are
T2702 53-56 DT denotes the
T2703 70-74 NN denotes ECFP
T2704 57-61 JJ denotes blue
T2705 62-69 VBN denotes shifted
T2706 61-62 HYPH denotes -
T2707 74-76 , denotes ,
T2708 76-79 CC denotes and
T2709 80-83 DT denotes the
T2710 96-100 NN denotes EYFP
T2711 84-87 JJ denotes red
T2712 88-95 VBN denotes shifted
T2713 87-88 HYPH denotes -
T2714 101-109 NNS denotes variants
T2715 110-112 IN denotes of
T2716 113-118 NN denotes wtGFP
T2717 123-127 RB denotes both
T2718 128-136 JJ denotes amenable
T2719 137-139 IN denotes to
T2720 140-143 NN denotes use
T2721 144-146 IN denotes in
T2722 147-151 NNS denotes mice
T2723 151-152 . denotes .
T2724 152-333 sentence denotes Also since we have established lines of viable and fertile mice having widespread FP expression we have demonstrated that ECFP and EYFP represent developmentally neutral reporters.
T2725 153-157 RB denotes Also
T2726 257-269 VBN denotes demonstrated
T2727 158-163 IN denotes since
T2728 172-183 VBN denotes established
T2729 164-166 PRP denotes we
T2730 167-171 VBP denotes have
T2731 184-189 NNS denotes lines
T2732 190-192 IN denotes of
T2733 193-199 JJ denotes viable
T2734 212-216 NNS denotes mice
T2735 200-203 CC denotes and
T2736 204-211 JJ denotes fertile
T2737 217-223 VBG denotes having
T2738 224-234 JJ denotes widespread
T2739 238-248 NN denotes expression
T2740 235-237 NN denotes FP
T2741 249-251 PRP denotes we
T2742 252-256 VBP denotes have
T2743 270-274 IN denotes that
T2744 289-298 VBP denotes represent
T2745 275-279 NN denotes ECFP
T2746 280-283 CC denotes and
T2747 284-288 NN denotes EYFP
T2748 299-314 RB denotes developmentally
T2749 315-322 JJ denotes neutral
T2750 323-332 NNS denotes reporters
T2751 332-333 . denotes .
T2752 333-652 sentence denotes Since we were unable to obtain ES cells, embryos or mice with widespread expression of a RFP (DsRed1) we conclude that this reporter is not developmentally neutral or that its expression cannot be sustained at levels high enough for detection, and as such is not as versatile as other gene-based fluorescent reporters.
T2753 334-339 IN denotes Since
T2754 343-347 VBD denotes were
T2755 340-342 PRP denotes we
T2756 439-447 VBP denotes conclude
T2757 348-354 JJ denotes unable
T2758 355-357 TO denotes to
T2759 358-364 VB denotes obtain
T2760 365-367 NN denotes ES
T2761 368-373 NNS denotes cells
T2762 373-375 , denotes ,
T2763 375-382 NNS denotes embryos
T2764 383-385 CC denotes or
T2765 386-390 NNS denotes mice
T2766 391-395 IN denotes with
T2767 396-406 JJ denotes widespread
T2768 407-417 NN denotes expression
T2769 418-420 IN denotes of
T2770 421-422 DT denotes a
T2771 423-426 NN denotes RFP
T2772 427-428 -LRB- denotes (
T2773 428-434 NN denotes DsRed1
T2774 434-435 -RRB- denotes )
T2775 436-438 PRP denotes we
T2776 448-452 IN denotes that
T2777 467-469 VBZ denotes is
T2778 453-457 DT denotes this
T2779 458-466 NN denotes reporter
T2780 470-473 RB denotes not
T2781 474-489 RB denotes developmentally
T2782 490-497 JJ denotes neutral
T2783 498-500 CC denotes or
T2784 501-505 IN denotes that
T2785 531-540 VBN denotes sustained
T2786 506-509 PRP$ denotes its
T2787 510-520 NN denotes expression
T2788 521-524 MD denotes can
T2789 524-527 RB denotes not
T2790 528-530 VB denotes be
T2791 541-543 IN denotes at
T2792 544-550 NNS denotes levels
T2793 551-555 JJ denotes high
T2794 556-562 JJ denotes enough
T2795 563-566 IN denotes for
T2796 567-576 NN denotes detection
T2797 576-578 , denotes ,
T2798 578-581 CC denotes and
T2799 582-584 IN denotes as
T2800 590-592 VBZ denotes is
T2801 585-589 JJ denotes such
T2802 593-596 RB denotes not
T2803 597-599 RB denotes as
T2804 600-609 JJ denotes versatile
T2805 610-612 IN denotes as
T2806 613-618 JJ denotes other
T2807 642-651 NNS denotes reporters
T2808 619-623 NN denotes gene
T2809 624-629 VBN denotes based
T2810 623-624 HYPH denotes -
T2811 630-641 JJ denotes fluorescent
T2812 651-652 . denotes .
T2813 652-933 sentence denotes ECFP and EYFP can be distinguished on the basis of their unique non-overlapping spectral profiles, and chimeras comprised of ECFP and EYFP tagged compartments demonstrate that the two reporters can be simultaneously non-invasively visualized within the same embryo or adult organ.
T2814 653-657 NN denotes ECFP
T2815 674-687 VBN denotes distinguished
T2816 658-661 CC denotes and
T2817 662-666 NN denotes EYFP
T2818 667-670 MD denotes can
T2819 671-673 VB denotes be
T2820 688-690 IN denotes on
T2821 691-694 DT denotes the
T2822 695-700 NN denotes basis
T2823 701-703 IN denotes of
T2824 704-709 PRP$ denotes their
T2825 742-750 NNS denotes profiles
T2826 710-716 JJ denotes unique
T2827 717-732 JJ denotes non-overlapping
T2828 733-741 JJ denotes spectral
T2829 750-752 , denotes ,
T2830 752-755 CC denotes and
T2831 756-764 NNS denotes chimeras
T2832 812-823 VBP denotes demonstrate
T2833 765-774 VBN denotes comprised
T2834 775-777 IN denotes of
T2835 778-782 NN denotes ECFP
T2836 792-798 VBN denotes tagged
T2837 783-786 CC denotes and
T2838 787-791 NN denotes EYFP
T2839 799-811 NNS denotes compartments
T2840 824-828 IN denotes that
T2841 884-894 VBN denotes visualized
T2842 829-832 DT denotes the
T2843 837-846 NNS denotes reporters
T2844 833-836 CD denotes two
T2845 847-850 MD denotes can
T2846 851-853 VB denotes be
T2847 854-868 RB denotes simultaneously
T2848 869-883 RB denotes non-invasively
T2849 895-901 IN denotes within
T2850 902-905 DT denotes the
T2851 911-917 NN denotes embryo
T2852 906-910 JJ denotes same
T2853 918-920 CC denotes or
T2854 921-926 JJ denotes adult
T2855 927-932 NN denotes organ
T2856 932-933 . denotes .
T2857 933-1082 sentence denotes Therefore it should be possible to use flow cytometry to isolate cells expressing either or both of the reporters from embryos or adult organs [14].
T2858 934-943 RB denotes Therefore
T2859 954-956 VB denotes be
T2860 944-946 PRP denotes it
T2861 947-953 MD denotes should
T2862 957-965 JJ denotes possible
T2863 966-968 TO denotes to
T2864 969-972 VB denotes use
T2865 973-977 NN denotes flow
T2866 978-987 NN denotes cytometry
T2867 988-990 TO denotes to
T2868 991-998 VB denotes isolate
T2869 999-1004 NNS denotes cells
T2870 1005-1015 VBG denotes expressing
T2871 1016-1022 DT denotes either
T2872 1023-1025 CC denotes or
T2873 1026-1030 DT denotes both
T2874 1031-1033 IN denotes of
T2875 1034-1037 DT denotes the
T2876 1038-1047 NNS denotes reporters
T2877 1048-1052 IN denotes from
T2878 1053-1060 NNS denotes embryos
T2879 1061-1063 CC denotes or
T2880 1064-1069 JJ denotes adult
T2881 1070-1076 NNS denotes organs
T2882 1077-1078 -LRB- denotes [
T2883 1078-1080 CD denotes 14
T2884 1080-1081 -RRB- denotes ]
T2885 1081-1082 . denotes .
T2886 1082-1213 sentence denotes These two reporters can also be used to create pairs of donors and acceptors for in vivo fluorescence energy transfer (FRET) [15].
T2887 1083-1088 DT denotes These
T2888 1093-1102 NNS denotes reporters
T2889 1089-1092 CD denotes two
T2890 1115-1119 VBN denotes used
T2891 1103-1106 MD denotes can
T2892 1107-1111 RB denotes also
T2893 1112-1114 VB denotes be
T2894 1120-1122 TO denotes to
T2895 1123-1129 VB denotes create
T2896 1130-1135 NNS denotes pairs
T2897 1136-1138 IN denotes of
T2898 1139-1145 NNS denotes donors
T2899 1146-1149 CC denotes and
T2900 1150-1159 NNS denotes acceptors
T2901 1160-1163 IN denotes for
T2902 1164-1166 FW denotes in
T2903 1167-1171 FW denotes vivo
T2904 1192-1200 NN denotes transfer
T2905 1172-1184 NN denotes fluorescence
T2906 1185-1191 NN denotes energy
T2907 1201-1202 -LRB- denotes (
T2908 1202-1206 NN denotes FRET
T2909 1206-1207 -RRB- denotes )
T2910 1208-1209 -LRB- denotes [
T2911 1209-1211 CD denotes 15
T2912 1211-1212 -RRB- denotes ]
T2913 1212-1213 . denotes .
T2914 1213-1515 sentence denotes Since the emission profiles of ECFP and EYFP are the least overlapping of all the usable GFP variants, these ECFP and EYFP currently represent the best combination for co-visualization and for use in multi-spectral double-tagged transgenic, chimeric and/or in vivo FRET experiments (reviewed in [16]).
T2915 1214-1219 IN denotes Since
T2916 1259-1262 VBP denotes are
T2917 1220-1223 DT denotes the
T2918 1233-1241 NNS denotes profiles
T2919 1224-1232 NN denotes emission
T2920 1242-1244 IN denotes of
T2921 1245-1249 NN denotes ECFP
T2922 1250-1253 CC denotes and
T2923 1254-1258 NN denotes EYFP
T2924 1347-1356 VBP denotes represent
T2925 1263-1266 DT denotes the
T2926 1273-1284 JJ denotes overlapping
T2927 1267-1272 RBS denotes least
T2928 1285-1287 IN denotes of
T2929 1288-1291 PDT denotes all
T2930 1307-1315 NNS denotes variants
T2931 1292-1295 DT denotes the
T2932 1296-1302 JJ denotes usable
T2933 1303-1306 NN denotes GFP
T2934 1315-1317 , denotes ,
T2935 1317-1322 DT denotes these
T2936 1323-1327 NN denotes ECFP
T2937 1328-1331 CC denotes and
T2938 1332-1336 NN denotes EYFP
T2939 1337-1346 RB denotes currently
T2940 1357-1360 DT denotes the
T2941 1366-1377 NN denotes combination
T2942 1361-1365 JJS denotes best
T2943 1378-1381 IN denotes for
T2944 1382-1398 NN denotes co-visualization
T2945 1399-1402 CC denotes and
T2946 1403-1406 IN denotes for
T2947 1407-1410 NN denotes use
T2948 1411-1413 IN denotes in
T2949 1414-1428 JJ denotes multi-spectral
T2950 1484-1495 NNS denotes experiments
T2951 1429-1435 JJ denotes double
T2952 1436-1442 JJ denotes tagged
T2953 1435-1436 HYPH denotes -
T2954 1443-1453 JJ denotes transgenic
T2955 1453-1455 , denotes ,
T2956 1455-1463 JJ denotes chimeric
T2957 1464-1467 CC denotes and
T2958 1467-1468 HYPH denotes /
T2959 1468-1470 CC denotes or
T2960 1471-1473 FW denotes in
T2961 1474-1478 FW denotes vivo
T2962 1479-1483 NN denotes FRET
T2963 1496-1497 -LRB- denotes (
T2964 1497-1505 VBN denotes reviewed
T2965 1506-1508 IN denotes in
T2966 1509-1510 -LRB- denotes [
T2967 1510-1512 CD denotes 16
T2968 1512-1513 -RRB- denotes ]
T2969 1513-1514 -RRB- denotes )
T2970 1514-1515 . denotes .
T2971 1515-1716 sentence denotes These experiments have demonstrated the feasibility of monitoring multiple FP reporters non-invasively within a single animal, and have shown the developmental neutrality of the FP reporters employed.
T2972 1516-1521 DT denotes These
T2973 1522-1533 NNS denotes experiments
T2974 1539-1551 VBN denotes demonstrated
T2975 1534-1538 VBP denotes have
T2976 1552-1555 DT denotes the
T2977 1556-1567 NN denotes feasibility
T2978 1568-1570 IN denotes of
T2979 1571-1581 VBG denotes monitoring
T2980 1582-1590 JJ denotes multiple
T2981 1594-1603 NNS denotes reporters
T2982 1591-1593 NN denotes FP
T2983 1604-1618 RB denotes non-invasively
T2984 1619-1625 IN denotes within
T2985 1626-1627 DT denotes a
T2986 1635-1641 NN denotes animal
T2987 1628-1634 JJ denotes single
T2988 1641-1643 , denotes ,
T2989 1643-1646 CC denotes and
T2990 1647-1651 VBP denotes have
T2991 1652-1657 VBN denotes shown
T2992 1658-1661 DT denotes the
T2993 1676-1686 NN denotes neutrality
T2994 1662-1675 JJ denotes developmental
T2995 1687-1689 IN denotes of
T2996 1690-1693 DT denotes the
T2997 1697-1706 NNS denotes reporters
T2998 1694-1696 NN denotes FP
T2999 1707-1715 VBN denotes employed
T3000 1715-1716 . denotes .
T3001 1716-1908 sentence denotes This should pave the way for novel strategies designed to incorporate the use of compound transgenics or tissue recombination or chimeric analyses where several compartments are to be tagged.
T3002 1717-1721 DT denotes This
T3003 1729-1733 VB denotes pave
T3004 1722-1728 MD denotes should
T3005 1734-1737 DT denotes the
T3006 1738-1741 NN denotes way
T3007 1742-1745 IN denotes for
T3008 1746-1751 JJ denotes novel
T3009 1752-1762 NNS denotes strategies
T3010 1763-1771 VBN denotes designed
T3011 1772-1774 TO denotes to
T3012 1775-1786 VB denotes incorporate
T3013 1787-1790 DT denotes the
T3014 1791-1794 NN denotes use
T3015 1795-1797 IN denotes of
T3016 1798-1806 NN denotes compound
T3017 1807-1818 NNS denotes transgenics
T3018 1819-1821 CC denotes or
T3019 1822-1828 NN denotes tissue
T3020 1829-1842 NN denotes recombination
T3021 1843-1845 CC denotes or
T3022 1846-1854 JJ denotes chimeric
T3023 1855-1863 NNS denotes analyses
T3024 1864-1869 WRB denotes where
T3025 1891-1894 VBP denotes are
T3026 1870-1877 JJ denotes several
T3027 1878-1890 NNS denotes compartments
T3028 1895-1897 TO denotes to
T3029 1901-1907 VBN denotes tagged
T3030 1898-1900 VB denotes be
T3031 1907-1908 . denotes .
T3032 1908-2037 sentence denotes This technology should provide new possibilities in manipulating the mouse genome, and non-invasively studying the consequences.
T3033 1909-1913 DT denotes This
T3034 1914-1924 NN denotes technology
T3035 1932-1939 VB denotes provide
T3036 1925-1931 MD denotes should
T3037 1940-1943 JJ denotes new
T3038 1944-1957 NNS denotes possibilities
T3039 1958-1960 IN denotes in
T3040 1961-1973 VBG denotes manipulating
T3041 1974-1977 DT denotes the
T3042 1984-1990 NN denotes genome
T3043 1978-1983 NN denotes mouse
T3044 1990-1992 , denotes ,
T3045 1992-1995 CC denotes and
T3046 1996-2010 RB denotes non-invasively
T3047 2011-2019 VBG denotes studying
T3048 2020-2023 DT denotes the
T3049 2024-2036 NNS denotes consequences
T3050 2036-2037 . denotes .
R1849 T2695 T2696 prep From,conclude
R1850 T2697 T2698 det these,observations
R1851 T2698 T2695 pobj observations,From
R1852 T2699 T2696 nsubj we,conclude
R1853 T2700 T2701 mark that,are
R1854 T2701 T2696 ccomp are,conclude
R1855 T2702 T2703 det the,ECFP
R1856 T2703 T2701 nsubj ECFP,are
R1857 T2704 T2705 amod blue,shifted
R1858 T2705 T2703 amod shifted,ECFP
R1859 T2706 T2705 punct -,shifted
R1860 T2707 T2703 punct ", ",ECFP
R1861 T2708 T2703 cc and,ECFP
R1862 T2709 T2710 det the,EYFP
R1863 T2710 T2714 compound EYFP,variants
R1864 T2711 T2712 amod red,shifted
R1865 T2712 T2710 amod shifted,EYFP
R1866 T2713 T2712 punct -,shifted
R1867 T2714 T2703 conj variants,ECFP
R1868 T2715 T2703 prep of,ECFP
R1869 T2716 T2715 pobj wtGFP,of
R1870 T2717 T2701 advmod both,are
R1871 T2718 T2701 acomp amenable,are
R1872 T2719 T2718 prep to,amenable
R1873 T2720 T2719 pobj use,to
R1874 T2721 T2720 prep in,use
R1875 T2722 T2721 pobj mice,in
R1876 T2723 T2696 punct .,conclude
R1877 T2725 T2726 advmod Also,demonstrated
R1878 T2727 T2728 mark since,established
R1879 T2728 T2726 advcl established,demonstrated
R1880 T2729 T2728 nsubj we,established
R1881 T2730 T2728 aux have,established
R1882 T2731 T2728 dobj lines,established
R1883 T2732 T2731 prep of,lines
R1884 T2733 T2734 amod viable,mice
R1885 T2734 T2732 pobj mice,of
R1886 T2735 T2733 cc and,viable
R1887 T2736 T2733 conj fertile,viable
R1888 T2737 T2731 acl having,lines
R1889 T2738 T2739 amod widespread,expression
R1890 T2739 T2737 dobj expression,having
R1891 T2740 T2739 compound FP,expression
R1892 T2741 T2726 nsubj we,demonstrated
R1893 T2742 T2726 aux have,demonstrated
R1894 T2743 T2744 mark that,represent
R1895 T2744 T2726 ccomp represent,demonstrated
R1896 T2745 T2744 nsubj ECFP,represent
R1897 T2746 T2745 cc and,ECFP
R1898 T2747 T2745 conj EYFP,ECFP
R1899 T2748 T2749 advmod developmentally,neutral
R1900 T2749 T2750 amod neutral,reporters
R1901 T2750 T2744 dobj reporters,represent
R1902 T2751 T2726 punct .,demonstrated
R1903 T2753 T2754 mark Since,were
R1904 T2754 T2756 advcl were,conclude
R1905 T2755 T2754 nsubj we,were
R1906 T2757 T2754 acomp unable,were
R1907 T2758 T2759 aux to,obtain
R1908 T2759 T2757 xcomp obtain,unable
R1909 T2760 T2761 compound ES,cells
R1910 T2761 T2759 dobj cells,obtain
R1911 T2762 T2761 punct ", ",cells
R1912 T2763 T2761 conj embryos,cells
R1913 T2764 T2763 cc or,embryos
R1914 T2765 T2763 conj mice,embryos
R1915 T2766 T2761 prep with,cells
R1916 T2767 T2768 amod widespread,expression
R1917 T2768 T2766 pobj expression,with
R1918 T2769 T2768 prep of,expression
R1919 T2770 T2771 det a,RFP
R1920 T2771 T2769 pobj RFP,of
R1921 T2772 T2771 punct (,RFP
R1922 T2773 T2771 appos DsRed1,RFP
R1923 T2774 T2756 punct ),conclude
R1924 T2775 T2756 nsubj we,conclude
R1925 T2776 T2777 mark that,is
R1926 T2777 T2756 advcl is,conclude
R1927 T2778 T2779 det this,reporter
R1928 T2779 T2777 nsubj reporter,is
R1929 T2780 T2777 neg not,is
R1930 T2781 T2782 advmod developmentally,neutral
R1931 T2782 T2777 acomp neutral,is
R1932 T2783 T2777 cc or,is
R1933 T2784 T2785 mark that,sustained
R1934 T2785 T2777 conj sustained,is
R1935 T2786 T2787 poss its,expression
R1936 T2787 T2785 nsubjpass expression,sustained
R1937 T2788 T2785 aux can,sustained
R1938 T2789 T2785 neg not,sustained
R1939 T2790 T2785 auxpass be,sustained
R1940 T2791 T2785 prep at,sustained
R1941 T2792 T2791 pobj levels,at
R1942 T2793 T2794 amod high,enough
R1943 T2794 T2792 amod enough,levels
R1944 T2795 T2794 prep for,enough
R1945 T2796 T2795 pobj detection,for
R1946 T2797 T2785 punct ", ",sustained
R1947 T2798 T2785 cc and,sustained
R1948 T2799 T2800 prep as,is
R1949 T2800 T2785 conj is,sustained
R1950 T2801 T2799 amod such,as
R1951 T2802 T2800 neg not,is
R1952 T2803 T2804 advmod as,versatile
R1953 T2804 T2800 acomp versatile,is
R1954 T2805 T2804 prep as,versatile
R1955 T2806 T2807 amod other,reporters
R1956 T2807 T2805 pobj reporters,as
R1957 T2808 T2809 npadvmod gene,based
R1958 T2809 T2807 amod based,reporters
R1959 T2810 T2809 punct -,based
R1960 T2811 T2807 amod fluorescent,reporters
R1961 T2812 T2756 punct .,conclude
R1962 T2814 T2815 nsubjpass ECFP,distinguished
R1963 T2816 T2814 cc and,ECFP
R1964 T2817 T2814 conj EYFP,ECFP
R1965 T2818 T2815 aux can,distinguished
R1966 T2819 T2815 auxpass be,distinguished
R1967 T2820 T2815 prep on,distinguished
R1968 T2821 T2822 det the,basis
R1969 T2822 T2820 pobj basis,on
R1970 T2823 T2822 prep of,basis
R1971 T2824 T2825 poss their,profiles
R1972 T2825 T2823 pobj profiles,of
R1973 T2826 T2825 amod unique,profiles
R1974 T2827 T2825 amod non-overlapping,profiles
R1975 T2828 T2825 amod spectral,profiles
R1976 T2829 T2815 punct ", ",distinguished
R1977 T2830 T2815 cc and,distinguished
R1978 T2831 T2832 nsubj chimeras,demonstrate
R1979 T2832 T2815 conj demonstrate,distinguished
R1980 T2833 T2831 acl comprised,chimeras
R1981 T2834 T2833 prep of,comprised
R1982 T2835 T2836 npadvmod ECFP,tagged
R1983 T2836 T2839 amod tagged,compartments
R1984 T2837 T2835 cc and,ECFP
R1985 T2838 T2835 conj EYFP,ECFP
R1986 T2839 T2834 pobj compartments,of
R1987 T2840 T2841 mark that,visualized
R1988 T2841 T2832 ccomp visualized,demonstrate
R1989 T2842 T2843 det the,reporters
R1990 T2843 T2841 nsubjpass reporters,visualized
R1991 T2844 T2843 nummod two,reporters
R1992 T2845 T2841 aux can,visualized
R1993 T2846 T2841 auxpass be,visualized
R1994 T2847 T2841 advmod simultaneously,visualized
R1995 T2848 T2841 advmod non-invasively,visualized
R1996 T2849 T2841 prep within,visualized
R1997 T2850 T2851 det the,embryo
R1998 T2851 T2849 pobj embryo,within
R1999 T2852 T2851 amod same,embryo
R2000 T2853 T2851 cc or,embryo
R2001 T2854 T2855 amod adult,organ
R2002 T2855 T2851 conj organ,embryo
R2003 T2856 T2832 punct .,demonstrate
R2004 T2858 T2859 advmod Therefore,be
R2005 T2860 T2859 nsubj it,be
R2006 T2861 T2859 aux should,be
R2007 T2862 T2859 acomp possible,be
R2008 T2863 T2864 aux to,use
R2009 T2864 T2859 xcomp use,be
R2010 T2865 T2866 compound flow,cytometry
R2011 T2866 T2864 dobj cytometry,use
R2012 T2867 T2868 aux to,isolate
R2013 T2868 T2864 advcl isolate,use
R2014 T2869 T2868 dobj cells,isolate
R2015 T2870 T2869 acl expressing,cells
R2016 T2871 T2870 dobj either,expressing
R2017 T2872 T2871 cc or,either
R2018 T2873 T2871 conj both,either
R2019 T2874 T2871 prep of,either
R2020 T2875 T2876 det the,reporters
R2021 T2876 T2874 pobj reporters,of
R2022 T2877 T2868 prep from,isolate
R2023 T2878 T2877 pobj embryos,from
R2024 T2879 T2878 cc or,embryos
R2025 T2880 T2881 amod adult,organs
R2026 T2881 T2878 conj organs,embryos
R2027 T2882 T2883 punct [,14
R2028 T2883 T2859 parataxis 14,be
R2029 T2884 T2883 punct ],14
R2030 T2885 T2859 punct .,be
R2031 T2887 T2888 det These,reporters
R2032 T2888 T2890 nsubjpass reporters,used
R2033 T2889 T2888 nummod two,reporters
R2034 T2891 T2890 aux can,used
R2035 T2892 T2890 advmod also,used
R2036 T2893 T2890 auxpass be,used
R2037 T2894 T2895 aux to,create
R2038 T2895 T2890 advcl create,used
R2039 T2896 T2895 dobj pairs,create
R2040 T2897 T2896 prep of,pairs
R2041 T2898 T2897 pobj donors,of
R2042 T2899 T2898 cc and,donors
R2043 T2900 T2898 conj acceptors,donors
R2044 T2901 T2895 prep for,create
R2045 T2902 T2903 advmod in,vivo
R2046 T2903 T2904 amod vivo,transfer
R2047 T2904 T2901 pobj transfer,for
R2048 T2905 T2904 compound fluorescence,transfer
R2049 T2906 T2904 compound energy,transfer
R2050 T2907 T2904 punct (,transfer
R2051 T2908 T2904 appos FRET,transfer
R2052 T2909 T2890 punct ),used
R2053 T2910 T2911 punct [,15
R2054 T2911 T2890 parataxis 15,used
R2055 T2912 T2911 punct ],15
R2056 T2913 T2890 punct .,used
R2057 T2915 T2916 mark Since,are
R2058 T2916 T2924 advcl are,represent
R2059 T2917 T2918 det the,profiles
R2060 T2918 T2916 nsubj profiles,are
R2061 T2919 T2918 compound emission,profiles
R2062 T2920 T2918 prep of,profiles
R2063 T2921 T2920 pobj ECFP,of
R2064 T2922 T2921 cc and,ECFP
R2065 T2923 T2921 conj EYFP,ECFP
R2066 T2925 T2926 det the,overlapping
R2067 T2926 T2916 attr overlapping,are
R2068 T2927 T2926 advmod least,overlapping
R2069 T2928 T2926 prep of,overlapping
R2070 T2929 T2930 predet all,variants
R2071 T2930 T2928 pobj variants,of
R2072 T2931 T2930 det the,variants
R2073 T2932 T2930 amod usable,variants
R2074 T2933 T2930 compound GFP,variants
R2075 T2934 T2924 punct ", ",represent
R2076 T2935 T2936 det these,ECFP
R2077 T2936 T2924 nsubj ECFP,represent
R2078 T2937 T2936 cc and,ECFP
R2079 T2938 T2936 conj EYFP,ECFP
R2080 T2939 T2924 advmod currently,represent
R2081 T2940 T2941 det the,combination
R2082 T2941 T2924 dobj combination,represent
R2083 T2942 T2941 amod best,combination
R2084 T2943 T2941 prep for,combination
R2085 T2944 T2943 pobj co-visualization,for
R2086 T2945 T2943 cc and,for
R2087 T2946 T2943 conj for,for
R2088 T2947 T2946 pobj use,for
R2089 T2948 T2947 prep in,use
R2090 T2949 T2950 amod multi-spectral,experiments
R2091 T2950 T2948 pobj experiments,in
R2092 T2951 T2952 amod double,tagged
R2093 T2952 T2950 amod tagged,experiments
R2094 T2953 T2952 punct -,tagged
R2095 T2954 T2950 amod transgenic,experiments
R2096 T2955 T2954 punct ", ",transgenic
R2097 T2956 T2954 conj chimeric,transgenic
R2098 T2957 T2956 cc and,chimeric
R2099 T2958 T2957 punct /,and
R2100 T2959 T2957 cc or,and
R2101 T2960 T2961 advmod in,vivo
R2102 T2961 T2956 conj vivo,chimeric
R2103 T2962 T2950 compound FRET,experiments
R2104 T2963 T2964 punct (,reviewed
R2105 T2964 T2924 parataxis reviewed,represent
R2106 T2965 T2964 prep in,reviewed
R2107 T2966 T2965 punct [,in
R2108 T2967 T2965 pobj 16,in
R2109 T2968 T2965 punct ],in
R2110 T2969 T2964 punct ),reviewed
R2111 T2970 T2924 punct .,represent
R2112 T2972 T2973 det These,experiments
R2113 T2973 T2974 nsubj experiments,demonstrated
R2114 T2975 T2974 aux have,demonstrated
R2115 T2976 T2977 det the,feasibility
R2116 T2977 T2974 dobj feasibility,demonstrated
R2117 T2978 T2977 prep of,feasibility
R2118 T2979 T2978 pcomp monitoring,of
R2119 T2980 T2981 amod multiple,reporters
R2120 T2981 T2979 dobj reporters,monitoring
R2121 T2982 T2981 compound FP,reporters
R2122 T2983 T2979 advmod non-invasively,monitoring
R2123 T2984 T2979 prep within,monitoring
R2124 T2985 T2986 det a,animal
R2125 T2986 T2984 pobj animal,within
R2126 T2987 T2986 amod single,animal
R2127 T2988 T2974 punct ", ",demonstrated
R2128 T2989 T2974 cc and,demonstrated
R2129 T2990 T2991 aux have,shown
R2130 T2991 T2974 conj shown,demonstrated
R2131 T2992 T2993 det the,neutrality
R2132 T2993 T2991 dobj neutrality,shown
R2133 T2994 T2993 amod developmental,neutrality
R2134 T2995 T2993 prep of,neutrality
R2135 T2996 T2997 det the,reporters
R2136 T2997 T2995 pobj reporters,of
R2137 T2998 T2997 compound FP,reporters
R2138 T2999 T2997 acl employed,reporters
R2139 T3000 T2974 punct .,demonstrated
R2140 T3002 T3003 nsubj This,pave
R2141 T3004 T3003 aux should,pave
R2142 T3005 T3006 det the,way
R2143 T3006 T3003 dobj way,pave
R2144 T3007 T3003 prep for,pave
R2145 T3008 T3009 amod novel,strategies
R2146 T3009 T3007 pobj strategies,for
R2147 T3010 T3009 acl designed,strategies
R2148 T3011 T3012 aux to,incorporate
R2149 T3012 T3010 advcl incorporate,designed
R2150 T3013 T3014 det the,use
R2151 T3014 T3012 dobj use,incorporate
R2152 T3015 T3014 prep of,use
R2153 T3016 T3017 compound compound,transgenics
R2154 T3017 T3015 pobj transgenics,of
R2155 T3018 T3017 cc or,transgenics
R2156 T3019 T3020 compound tissue,recombination
R2157 T3020 T3017 conj recombination,transgenics
R2158 T3021 T3020 cc or,recombination
R2159 T3022 T3023 amod chimeric,analyses
R2160 T3023 T3020 conj analyses,recombination
R2161 T3024 T3025 advmod where,are
R2162 T3025 T3014 relcl are,use
R2163 T3026 T3027 amod several,compartments
R2164 T3027 T3025 nsubj compartments,are
R2165 T3028 T3029 aux to,tagged
R2166 T3029 T3025 xcomp tagged,are
R2167 T3030 T3029 auxpass be,tagged
R2168 T3031 T3003 punct .,pave
R2169 T3033 T3034 det This,technology
R2170 T3034 T3035 nsubj technology,provide
R2171 T3036 T3035 aux should,provide
R2172 T3037 T3038 amod new,possibilities
R2173 T3038 T3035 dobj possibilities,provide
R2174 T3039 T3038 prep in,possibilities
R2175 T3040 T3039 pcomp manipulating,in
R2176 T3041 T3042 det the,genome
R2177 T3042 T3040 dobj genome,manipulating
R2178 T3043 T3042 compound mouse,genome
R2179 T3044 T3040 punct ", ",manipulating
R2180 T3045 T3040 cc and,manipulating
R2181 T3046 T3047 advmod non-invasively,studying
R2182 T3047 T3040 conj studying,manipulating
R2183 T3048 T3049 det the,consequences
R2184 T3049 T3047 dobj consequences,studying
R2185 T3050 T3035 punct .,provide

craft-ca-core-ex-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T2664 101-109 SO_EXT:sequence_variant_entity denotes variants
T2665 147-151 NCBITaxon:10088 denotes mice
T2666 212-216 NCBITaxon:10088 denotes mice
T2667 238-248 GO:0010467 denotes expression
T2668 365-373 CL:0002322 denotes ES cells
T2669 368-373 CL_GO_EXT:cell denotes cells
T2670 375-382 UBERON:0000922 denotes embryos
T2671 386-390 NCBITaxon:10088 denotes mice
T2672 407-417 GO:0010467 denotes expression
T2673 510-520 GO:0010467 denotes expression
T2674 619-623 SO_EXT:0000704 denotes gene
T2675 792-798 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes tagged
T2676 911-917 UBERON:0000922 denotes embryo
T2677 921-926 UBERON:0007023 denotes adult
T2678 927-932 UBERON:0000062 denotes organ
T2679 999-1004 CL_GO_EXT:cell denotes cells
T2680 1005-1015 GO:0010467 denotes expressing
T2681 1053-1060 UBERON:0000922 denotes embryos
T2682 1064-1069 UBERON:0007023 denotes adult
T2683 1070-1076 UBERON:0000062 denotes organs
T2684 1192-1200 GO_EXT:biological_movement_or_translocation_process denotes transfer
T2685 1307-1315 SO_EXT:sequence_variant_entity denotes variants
T2686 1436-1442 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes tagged
T2687 1443-1453 SO_EXT:transgenic_entity denotes transgenic
T2688 1635-1641 NCBITaxon:33208 denotes animal
T2689 1807-1818 SO_EXT:transgenic_entity denotes transgenics
T2690 1822-1828 UBERON:0000479 denotes tissue
T2691 1901-1907 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes tagged
T2692 1961-1973 SO_EXT:sequence_alteration_process denotes manipulating
T2693 1978-1983 NCBITaxon:10088 denotes mouse
T2694 1984-1990 SO_EXT:0001026 denotes genome

craft-ca-core-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T2644 147-151 NCBITaxon:10088 denotes mice
T2645 212-216 NCBITaxon:10088 denotes mice
T2646 238-248 GO:0010467 denotes expression
T2647 365-373 CL:0002322 denotes ES cells
T2648 375-382 UBERON:0000922 denotes embryos
T2649 386-390 NCBITaxon:10088 denotes mice
T2650 407-417 GO:0010467 denotes expression
T2651 510-520 GO:0010467 denotes expression
T2652 619-623 SO:0000704 denotes gene
T2653 911-917 UBERON:0000922 denotes embryo
T2654 921-926 UBERON:0007023 denotes adult
T2655 927-932 UBERON:0000062 denotes organ
T2656 1005-1015 GO:0010467 denotes expressing
T2657 1053-1060 UBERON:0000922 denotes embryos
T2658 1064-1069 UBERON:0007023 denotes adult
T2659 1070-1076 UBERON:0000062 denotes organs
T2660 1635-1641 NCBITaxon:33208 denotes animal
T2661 1822-1828 UBERON:0000479 denotes tissue
T2662 1978-1983 NCBITaxon:10088 denotes mouse
T2663 1984-1990 SO:0001026 denotes genome

2_test

Id Subject Object Predicate Lexical cue
12079497-10951501-8577609 1078-1080 10951501 denotes 14
12079497-10087619-8577610 1209-1211 10087619 denotes 15
12079497-11219608-8577611 1510-1512 11219608 denotes 16