Id |
Subject |
Object |
Predicate |
Lexical cue |
T180 |
0-12 |
JJ |
denotes |
Non-invasive |
T181 |
29-38 |
NNS |
denotes |
reporters |
T182 |
13-28 |
JJ |
denotes |
autofluorescent |
T183 |
44-58 |
VBN |
denotes |
revolutionized |
T184 |
39-43 |
VBP |
denotes |
have |
T185 |
59-66 |
NN |
denotes |
lineage |
T186 |
67-75 |
NN |
denotes |
labeling |
T187 |
76-78 |
IN |
denotes |
in |
T188 |
79-81 |
DT |
denotes |
an |
T189 |
82-87 |
NN |
denotes |
array |
T190 |
88-90 |
IN |
denotes |
of |
T191 |
91-100 |
JJ |
denotes |
different |
T192 |
101-110 |
NNS |
denotes |
organisms |
T193 |
110-111 |
. |
denotes |
. |
T194 |
111-285 |
sentence |
denotes |
In recent years green fluorescent protein (GFP) from the bioluminescent jellyfish Aequoria Victoria has gained popularity in mouse transgenic and gene targeting regimes [1]. |
T195 |
112-114 |
IN |
denotes |
In |
T196 |
216-222 |
VBN |
denotes |
gained |
T197 |
115-121 |
JJ |
denotes |
recent |
T198 |
122-127 |
NNS |
denotes |
years |
T199 |
128-133 |
JJ |
denotes |
green |
T200 |
146-153 |
NN |
denotes |
protein |
T201 |
134-145 |
JJ |
denotes |
fluorescent |
T202 |
154-155 |
-LRB- |
denotes |
( |
T203 |
155-158 |
NN |
denotes |
GFP |
T204 |
158-159 |
-RRB- |
denotes |
) |
T205 |
160-164 |
IN |
denotes |
from |
T206 |
165-168 |
DT |
denotes |
the |
T207 |
184-193 |
NN |
denotes |
jellyfish |
T208 |
169-183 |
JJ |
denotes |
bioluminescent |
T209 |
194-202 |
NNP |
denotes |
Aequoria |
T210 |
203-211 |
NNP |
denotes |
Victoria |
T211 |
212-215 |
VBZ |
denotes |
has |
T212 |
223-233 |
NN |
denotes |
popularity |
T213 |
234-236 |
IN |
denotes |
in |
T214 |
237-242 |
NN |
denotes |
mouse |
T215 |
243-253 |
JJ |
denotes |
transgenic |
T216 |
273-280 |
NNS |
denotes |
regimes |
T217 |
254-257 |
CC |
denotes |
and |
T218 |
258-262 |
NN |
denotes |
gene |
T219 |
263-272 |
VBG |
denotes |
targeting |
T220 |
281-282 |
-LRB- |
denotes |
[ |
T221 |
282-283 |
CD |
denotes |
1 |
T222 |
283-284 |
-RRB- |
denotes |
] |
T223 |
284-285 |
. |
denotes |
. |
T224 |
285-489 |
sentence |
denotes |
It offers several advantages over conventional gene-based reporters, such as lacZ and alkaline phosphatase, in that its visualization does not require a chromogenic substrate and can be realized in vivo. |
T225 |
286-288 |
PRP |
denotes |
It |
T226 |
289-295 |
VBZ |
denotes |
offers |
T227 |
296-303 |
JJ |
denotes |
several |
T228 |
304-314 |
NNS |
denotes |
advantages |
T229 |
315-319 |
IN |
denotes |
over |
T230 |
320-332 |
JJ |
denotes |
conventional |
T231 |
344-353 |
NNS |
denotes |
reporters |
T232 |
333-337 |
NN |
denotes |
gene |
T233 |
338-343 |
VBN |
denotes |
based |
T234 |
337-338 |
HYPH |
denotes |
- |
T235 |
353-355 |
, |
denotes |
, |
T236 |
355-359 |
JJ |
denotes |
such |
T237 |
360-362 |
IN |
denotes |
as |
T238 |
363-367 |
NN |
denotes |
lacZ |
T239 |
368-371 |
CC |
denotes |
and |
T240 |
372-380 |
NN |
denotes |
alkaline |
T241 |
381-392 |
NN |
denotes |
phosphatase |
T242 |
392-394 |
, |
denotes |
, |
T243 |
394-396 |
IN |
denotes |
in |
T244 |
429-436 |
VB |
denotes |
require |
T245 |
397-401 |
IN |
denotes |
that |
T246 |
402-405 |
PRP$ |
denotes |
its |
T247 |
406-419 |
NN |
denotes |
visualization |
T248 |
420-424 |
VBZ |
denotes |
does |
T249 |
425-428 |
RB |
denotes |
not |
T250 |
437-438 |
DT |
denotes |
a |
T251 |
451-460 |
NN |
denotes |
substrate |
T252 |
439-450 |
JJ |
denotes |
chromogenic |
T253 |
461-464 |
CC |
denotes |
and |
T254 |
465-468 |
MD |
denotes |
can |
T255 |
472-480 |
VBN |
denotes |
realized |
T256 |
469-471 |
VB |
denotes |
be |
T257 |
481-483 |
FW |
denotes |
in |
T258 |
484-488 |
FW |
denotes |
vivo |
T259 |
488-489 |
. |
denotes |
. |
T260 |
489-650 |
sentence |
denotes |
We have previously demonstrated the utility and developmental neutrality of enhanced green fluorescent protein (EGFP) in embryonic stem (ES) cells and mice [2]. |
T261 |
490-492 |
PRP |
denotes |
We |
T262 |
509-521 |
VBN |
denotes |
demonstrated |
T263 |
493-497 |
VBP |
denotes |
have |
T264 |
498-508 |
RB |
denotes |
previously |
T265 |
522-525 |
DT |
denotes |
the |
T266 |
526-533 |
NN |
denotes |
utility |
T267 |
534-537 |
CC |
denotes |
and |
T268 |
538-551 |
JJ |
denotes |
developmental |
T269 |
552-562 |
NN |
denotes |
neutrality |
T270 |
563-565 |
IN |
denotes |
of |
T271 |
566-574 |
VBN |
denotes |
enhanced |
T272 |
593-600 |
NN |
denotes |
protein |
T273 |
575-580 |
JJ |
denotes |
green |
T274 |
581-592 |
JJ |
denotes |
fluorescent |
T275 |
601-602 |
-LRB- |
denotes |
( |
T276 |
602-606 |
NN |
denotes |
EGFP |
T277 |
606-607 |
-RRB- |
denotes |
) |
T278 |
608-610 |
IN |
denotes |
in |
T279 |
611-620 |
JJ |
denotes |
embryonic |
T280 |
621-625 |
NN |
denotes |
stem |
T281 |
631-636 |
NNS |
denotes |
cells |
T282 |
626-627 |
-LRB- |
denotes |
( |
T283 |
627-629 |
NN |
denotes |
ES |
T284 |
629-630 |
-RRB- |
denotes |
) |
T285 |
637-640 |
CC |
denotes |
and |
T286 |
641-645 |
NNS |
denotes |
mice |
T287 |
646-647 |
-LRB- |
denotes |
[ |
T288 |
647-648 |
CD |
denotes |
2 |
T289 |
648-649 |
-RRB- |
denotes |
] |
T290 |
649-650 |
. |
denotes |
. |
R25 |
T180 |
T181 |
amod |
Non-invasive,reporters |
R26 |
T181 |
T183 |
nsubj |
reporters,revolutionized |
R27 |
T182 |
T181 |
amod |
autofluorescent,reporters |
R28 |
T184 |
T183 |
aux |
have,revolutionized |
R29 |
T185 |
T186 |
compound |
lineage,labeling |
R30 |
T186 |
T183 |
dobj |
labeling,revolutionized |
R31 |
T187 |
T183 |
prep |
in,revolutionized |
R32 |
T188 |
T189 |
det |
an,array |
R33 |
T189 |
T187 |
pobj |
array,in |
R34 |
T190 |
T189 |
prep |
of,array |
R35 |
T191 |
T192 |
amod |
different,organisms |
R36 |
T192 |
T190 |
pobj |
organisms,of |
R37 |
T193 |
T183 |
punct |
.,revolutionized |
R38 |
T195 |
T196 |
prep |
In,gained |
R39 |
T197 |
T198 |
amod |
recent,years |
R40 |
T198 |
T195 |
pobj |
years,In |
R41 |
T199 |
T200 |
amod |
green,protein |
R42 |
T200 |
T196 |
nsubj |
protein,gained |
R43 |
T201 |
T200 |
amod |
fluorescent,protein |
R44 |
T202 |
T200 |
punct |
(,protein |
R45 |
T203 |
T200 |
appos |
GFP,protein |
R46 |
T204 |
T200 |
punct |
),protein |
R47 |
T205 |
T200 |
prep |
from,protein |
R48 |
T206 |
T207 |
det |
the,jellyfish |
R49 |
T207 |
T205 |
pobj |
jellyfish,from |
R50 |
T208 |
T207 |
amod |
bioluminescent,jellyfish |
R51 |
T209 |
T210 |
compound |
Aequoria,Victoria |
R52 |
T210 |
T207 |
appos |
Victoria,jellyfish |
R53 |
T211 |
T196 |
aux |
has,gained |
R54 |
T212 |
T196 |
dobj |
popularity,gained |
R55 |
T213 |
T196 |
prep |
in,gained |
R56 |
T214 |
T215 |
npadvmod |
mouse,transgenic |
R57 |
T215 |
T216 |
amod |
transgenic,regimes |
R58 |
T216 |
T213 |
pobj |
regimes,in |
R59 |
T217 |
T215 |
cc |
and,transgenic |
R60 |
T218 |
T219 |
npadvmod |
gene,targeting |
R61 |
T219 |
T215 |
conj |
targeting,transgenic |
R62 |
T220 |
T221 |
punct |
[,1 |
R63 |
T221 |
T196 |
parataxis |
1,gained |
R64 |
T222 |
T221 |
punct |
],1 |
R65 |
T223 |
T196 |
punct |
.,gained |
R66 |
T225 |
T226 |
nsubj |
It,offers |
R67 |
T227 |
T228 |
amod |
several,advantages |
R68 |
T228 |
T226 |
dobj |
advantages,offers |
R69 |
T229 |
T228 |
prep |
over,advantages |
R70 |
T230 |
T231 |
amod |
conventional,reporters |
R71 |
T231 |
T229 |
pobj |
reporters,over |
R72 |
T232 |
T233 |
npadvmod |
gene,based |
R73 |
T233 |
T231 |
amod |
based,reporters |
R74 |
T234 |
T233 |
punct |
-,based |
R75 |
T235 |
T228 |
punct |
", ",advantages |
R76 |
T236 |
T237 |
amod |
such,as |
R77 |
T237 |
T228 |
prep |
as,advantages |
R78 |
T238 |
T237 |
pobj |
lacZ,as |
R79 |
T239 |
T238 |
cc |
and,lacZ |
R80 |
T240 |
T241 |
compound |
alkaline,phosphatase |
R81 |
T241 |
T238 |
conj |
phosphatase,lacZ |
R82 |
T242 |
T226 |
punct |
", ",offers |
R83 |
T243 |
T244 |
mark |
in,require |
R84 |
T244 |
T226 |
advcl |
require,offers |
R85 |
T245 |
T244 |
mark |
that,require |
R86 |
T246 |
T247 |
poss |
its,visualization |
R87 |
T247 |
T244 |
nsubj |
visualization,require |
R88 |
T248 |
T244 |
aux |
does,require |
R89 |
T249 |
T244 |
neg |
not,require |
R90 |
T250 |
T251 |
det |
a,substrate |
R91 |
T251 |
T244 |
dobj |
substrate,require |
R92 |
T252 |
T251 |
amod |
chromogenic,substrate |
R93 |
T253 |
T244 |
cc |
and,require |
R94 |
T254 |
T255 |
aux |
can,realized |
R95 |
T255 |
T244 |
conj |
realized,require |
R96 |
T256 |
T255 |
auxpass |
be,realized |
R97 |
T257 |
T258 |
advmod |
in,vivo |
R98 |
T258 |
T255 |
advmod |
vivo,realized |
R99 |
T259 |
T226 |
punct |
.,offers |
R100 |
T261 |
T262 |
nsubj |
We,demonstrated |
R101 |
T263 |
T262 |
aux |
have,demonstrated |
R102 |
T264 |
T262 |
advmod |
previously,demonstrated |
R103 |
T265 |
T266 |
det |
the,utility |
R104 |
T266 |
T262 |
dobj |
utility,demonstrated |
R105 |
T267 |
T266 |
cc |
and,utility |
R106 |
T268 |
T269 |
amod |
developmental,neutrality |
R107 |
T269 |
T266 |
conj |
neutrality,utility |
R108 |
T270 |
T266 |
prep |
of,utility |
R109 |
T271 |
T272 |
amod |
enhanced,protein |
R110 |
T272 |
T270 |
pobj |
protein,of |
R111 |
T273 |
T272 |
amod |
green,protein |
R112 |
T274 |
T272 |
amod |
fluorescent,protein |
R113 |
T275 |
T272 |
punct |
(,protein |
R114 |
T276 |
T272 |
appos |
EGFP,protein |
R115 |
T277 |
T266 |
punct |
),utility |
R116 |
T278 |
T266 |
prep |
in,utility |
R117 |
T279 |
T280 |
amod |
embryonic,stem |
R118 |
T280 |
T281 |
nmod |
stem,cells |
R119 |
T281 |
T278 |
pobj |
cells,in |
R120 |
T282 |
T280 |
punct |
(,stem |
R121 |
T283 |
T280 |
appos |
ES,stem |
R122 |
T284 |
T281 |
punct |
),cells |
R123 |
T285 |
T281 |
cc |
and,cells |
R124 |
T286 |
T281 |
conj |
mice,cells |
R125 |
T287 |
T288 |
punct |
[,2 |
R126 |
T288 |
T262 |
parataxis |
2,demonstrated |
R127 |
T289 |
T288 |
punct |
],2 |
R128 |
T290 |
T262 |
punct |
.,demonstrated |