Id |
Subject |
Object |
Predicate |
Lexical cue |
T177 |
0-10 |
sentence |
denotes |
Background |
T178 |
0-10 |
NN |
denotes |
Background |
T179 |
10-122 |
sentence |
denotes |
Non-invasive autofluorescent reporters have revolutionized lineage labeling in an array of different organisms. |
T180 |
11-23 |
JJ |
denotes |
Non-invasive |
T181 |
40-49 |
NNS |
denotes |
reporters |
T182 |
24-39 |
JJ |
denotes |
autofluorescent |
T183 |
55-69 |
VBN |
denotes |
revolutionized |
T184 |
50-54 |
VBP |
denotes |
have |
T185 |
70-77 |
NN |
denotes |
lineage |
T186 |
78-86 |
NN |
denotes |
labeling |
T187 |
87-89 |
IN |
denotes |
in |
T188 |
90-92 |
DT |
denotes |
an |
T189 |
93-98 |
NN |
denotes |
array |
T190 |
99-101 |
IN |
denotes |
of |
T191 |
102-111 |
JJ |
denotes |
different |
T192 |
112-121 |
NNS |
denotes |
organisms |
T193 |
121-122 |
. |
denotes |
. |
T194 |
122-296 |
sentence |
denotes |
In recent years green fluorescent protein (GFP) from the bioluminescent jellyfish Aequoria Victoria has gained popularity in mouse transgenic and gene targeting regimes [1]. |
T195 |
123-125 |
IN |
denotes |
In |
T196 |
227-233 |
VBN |
denotes |
gained |
T197 |
126-132 |
JJ |
denotes |
recent |
T198 |
133-138 |
NNS |
denotes |
years |
T199 |
139-144 |
JJ |
denotes |
green |
T200 |
157-164 |
NN |
denotes |
protein |
T201 |
145-156 |
JJ |
denotes |
fluorescent |
T202 |
165-166 |
-LRB- |
denotes |
( |
T203 |
166-169 |
NN |
denotes |
GFP |
T204 |
169-170 |
-RRB- |
denotes |
) |
T205 |
171-175 |
IN |
denotes |
from |
T206 |
176-179 |
DT |
denotes |
the |
T207 |
195-204 |
NN |
denotes |
jellyfish |
T208 |
180-194 |
JJ |
denotes |
bioluminescent |
T209 |
205-213 |
NNP |
denotes |
Aequoria |
T210 |
214-222 |
NNP |
denotes |
Victoria |
T211 |
223-226 |
VBZ |
denotes |
has |
T212 |
234-244 |
NN |
denotes |
popularity |
T213 |
245-247 |
IN |
denotes |
in |
T214 |
248-253 |
NN |
denotes |
mouse |
T215 |
254-264 |
JJ |
denotes |
transgenic |
T216 |
284-291 |
NNS |
denotes |
regimes |
T217 |
265-268 |
CC |
denotes |
and |
T218 |
269-273 |
NN |
denotes |
gene |
T219 |
274-283 |
VBG |
denotes |
targeting |
T220 |
292-293 |
-LRB- |
denotes |
[ |
T221 |
293-294 |
CD |
denotes |
1 |
T222 |
294-295 |
-RRB- |
denotes |
] |
T223 |
295-296 |
. |
denotes |
. |
T224 |
296-500 |
sentence |
denotes |
It offers several advantages over conventional gene-based reporters, such as lacZ and alkaline phosphatase, in that its visualization does not require a chromogenic substrate and can be realized in vivo. |
T225 |
297-299 |
PRP |
denotes |
It |
T226 |
300-306 |
VBZ |
denotes |
offers |
T227 |
307-314 |
JJ |
denotes |
several |
T228 |
315-325 |
NNS |
denotes |
advantages |
T229 |
326-330 |
IN |
denotes |
over |
T230 |
331-343 |
JJ |
denotes |
conventional |
T231 |
355-364 |
NNS |
denotes |
reporters |
T232 |
344-348 |
NN |
denotes |
gene |
T233 |
349-354 |
VBN |
denotes |
based |
T234 |
348-349 |
HYPH |
denotes |
- |
T235 |
364-366 |
, |
denotes |
, |
T236 |
366-370 |
JJ |
denotes |
such |
T237 |
371-373 |
IN |
denotes |
as |
T238 |
374-378 |
NN |
denotes |
lacZ |
T239 |
379-382 |
CC |
denotes |
and |
T240 |
383-391 |
NN |
denotes |
alkaline |
T241 |
392-403 |
NN |
denotes |
phosphatase |
T242 |
403-405 |
, |
denotes |
, |
T243 |
405-407 |
IN |
denotes |
in |
T244 |
440-447 |
VB |
denotes |
require |
T245 |
408-412 |
IN |
denotes |
that |
T246 |
413-416 |
PRP$ |
denotes |
its |
T247 |
417-430 |
NN |
denotes |
visualization |
T248 |
431-435 |
VBZ |
denotes |
does |
T249 |
436-439 |
RB |
denotes |
not |
T250 |
448-449 |
DT |
denotes |
a |
T251 |
462-471 |
NN |
denotes |
substrate |
T252 |
450-461 |
JJ |
denotes |
chromogenic |
T253 |
472-475 |
CC |
denotes |
and |
T254 |
476-479 |
MD |
denotes |
can |
T255 |
483-491 |
VBN |
denotes |
realized |
T256 |
480-482 |
VB |
denotes |
be |
T257 |
492-494 |
FW |
denotes |
in |
T258 |
495-499 |
FW |
denotes |
vivo |
T259 |
499-500 |
. |
denotes |
. |
T260 |
500-661 |
sentence |
denotes |
We have previously demonstrated the utility and developmental neutrality of enhanced green fluorescent protein (EGFP) in embryonic stem (ES) cells and mice [2]. |
T261 |
501-503 |
PRP |
denotes |
We |
T262 |
520-532 |
VBN |
denotes |
demonstrated |
T263 |
504-508 |
VBP |
denotes |
have |
T264 |
509-519 |
RB |
denotes |
previously |
T265 |
533-536 |
DT |
denotes |
the |
T266 |
537-544 |
NN |
denotes |
utility |
T267 |
545-548 |
CC |
denotes |
and |
T268 |
549-562 |
JJ |
denotes |
developmental |
T269 |
563-573 |
NN |
denotes |
neutrality |
T270 |
574-576 |
IN |
denotes |
of |
T271 |
577-585 |
VBN |
denotes |
enhanced |
T272 |
604-611 |
NN |
denotes |
protein |
T273 |
586-591 |
JJ |
denotes |
green |
T274 |
592-603 |
JJ |
denotes |
fluorescent |
T275 |
612-613 |
-LRB- |
denotes |
( |
T276 |
613-617 |
NN |
denotes |
EGFP |
T277 |
617-618 |
-RRB- |
denotes |
) |
T278 |
619-621 |
IN |
denotes |
in |
T279 |
622-631 |
JJ |
denotes |
embryonic |
T280 |
632-636 |
NN |
denotes |
stem |
T281 |
642-647 |
NNS |
denotes |
cells |
T282 |
637-638 |
-LRB- |
denotes |
( |
T283 |
638-640 |
NN |
denotes |
ES |
T284 |
640-641 |
-RRB- |
denotes |
) |
T285 |
648-651 |
CC |
denotes |
and |
T286 |
652-656 |
NNS |
denotes |
mice |
T287 |
657-658 |
-LRB- |
denotes |
[ |
T288 |
658-659 |
CD |
denotes |
2 |
T289 |
659-660 |
-RRB- |
denotes |
] |
T290 |
660-661 |
. |
denotes |
. |
R25 |
T180 |
T181 |
amod |
Non-invasive,reporters |
R26 |
T181 |
T183 |
nsubj |
reporters,revolutionized |
R27 |
T182 |
T181 |
amod |
autofluorescent,reporters |
R28 |
T184 |
T183 |
aux |
have,revolutionized |
R29 |
T185 |
T186 |
compound |
lineage,labeling |
R30 |
T186 |
T183 |
dobj |
labeling,revolutionized |
R31 |
T187 |
T183 |
prep |
in,revolutionized |
R32 |
T188 |
T189 |
det |
an,array |
R33 |
T189 |
T187 |
pobj |
array,in |
R34 |
T190 |
T189 |
prep |
of,array |
R35 |
T191 |
T192 |
amod |
different,organisms |
R36 |
T192 |
T190 |
pobj |
organisms,of |
R37 |
T193 |
T183 |
punct |
.,revolutionized |
R38 |
T195 |
T196 |
prep |
In,gained |
R39 |
T197 |
T198 |
amod |
recent,years |
R40 |
T198 |
T195 |
pobj |
years,In |
R41 |
T199 |
T200 |
amod |
green,protein |
R42 |
T200 |
T196 |
nsubj |
protein,gained |
R43 |
T201 |
T200 |
amod |
fluorescent,protein |
R44 |
T202 |
T200 |
punct |
(,protein |
R45 |
T203 |
T200 |
appos |
GFP,protein |
R46 |
T204 |
T200 |
punct |
),protein |
R47 |
T205 |
T200 |
prep |
from,protein |
R48 |
T206 |
T207 |
det |
the,jellyfish |
R49 |
T207 |
T205 |
pobj |
jellyfish,from |
R50 |
T208 |
T207 |
amod |
bioluminescent,jellyfish |
R51 |
T209 |
T210 |
compound |
Aequoria,Victoria |
R52 |
T210 |
T207 |
appos |
Victoria,jellyfish |
R53 |
T211 |
T196 |
aux |
has,gained |
R54 |
T212 |
T196 |
dobj |
popularity,gained |
R55 |
T213 |
T196 |
prep |
in,gained |
R56 |
T214 |
T215 |
npadvmod |
mouse,transgenic |
R57 |
T215 |
T216 |
amod |
transgenic,regimes |
R58 |
T216 |
T213 |
pobj |
regimes,in |
R59 |
T217 |
T215 |
cc |
and,transgenic |
R60 |
T218 |
T219 |
npadvmod |
gene,targeting |
R61 |
T219 |
T215 |
conj |
targeting,transgenic |
R62 |
T220 |
T221 |
punct |
[,1 |
R63 |
T221 |
T196 |
parataxis |
1,gained |
R64 |
T222 |
T221 |
punct |
],1 |
R65 |
T223 |
T196 |
punct |
.,gained |
R66 |
T225 |
T226 |
nsubj |
It,offers |
R67 |
T227 |
T228 |
amod |
several,advantages |
R68 |
T228 |
T226 |
dobj |
advantages,offers |
R69 |
T229 |
T228 |
prep |
over,advantages |
R70 |
T230 |
T231 |
amod |
conventional,reporters |
R71 |
T231 |
T229 |
pobj |
reporters,over |
R72 |
T232 |
T233 |
npadvmod |
gene,based |
R73 |
T233 |
T231 |
amod |
based,reporters |
R74 |
T234 |
T233 |
punct |
-,based |
R75 |
T235 |
T228 |
punct |
", ",advantages |
R76 |
T236 |
T237 |
amod |
such,as |
R77 |
T237 |
T228 |
prep |
as,advantages |
R78 |
T238 |
T237 |
pobj |
lacZ,as |
R79 |
T239 |
T238 |
cc |
and,lacZ |
R80 |
T240 |
T241 |
compound |
alkaline,phosphatase |
R81 |
T241 |
T238 |
conj |
phosphatase,lacZ |
R82 |
T242 |
T226 |
punct |
", ",offers |
R83 |
T243 |
T244 |
mark |
in,require |
R84 |
T244 |
T226 |
advcl |
require,offers |
R85 |
T245 |
T244 |
mark |
that,require |
R86 |
T246 |
T247 |
poss |
its,visualization |
R87 |
T247 |
T244 |
nsubj |
visualization,require |
R88 |
T248 |
T244 |
aux |
does,require |
R89 |
T249 |
T244 |
neg |
not,require |
R90 |
T250 |
T251 |
det |
a,substrate |
R91 |
T251 |
T244 |
dobj |
substrate,require |
R92 |
T252 |
T251 |
amod |
chromogenic,substrate |
R93 |
T253 |
T244 |
cc |
and,require |
R94 |
T254 |
T255 |
aux |
can,realized |
R95 |
T255 |
T244 |
conj |
realized,require |
R96 |
T256 |
T255 |
auxpass |
be,realized |
R97 |
T257 |
T258 |
advmod |
in,vivo |
R98 |
T258 |
T255 |
advmod |
vivo,realized |
R99 |
T259 |
T226 |
punct |
.,offers |
R100 |
T261 |
T262 |
nsubj |
We,demonstrated |
R101 |
T263 |
T262 |
aux |
have,demonstrated |
R102 |
T264 |
T262 |
advmod |
previously,demonstrated |
R103 |
T265 |
T266 |
det |
the,utility |
R104 |
T266 |
T262 |
dobj |
utility,demonstrated |
R105 |
T267 |
T266 |
cc |
and,utility |
R106 |
T268 |
T269 |
amod |
developmental,neutrality |
R107 |
T269 |
T266 |
conj |
neutrality,utility |
R108 |
T270 |
T266 |
prep |
of,utility |
R109 |
T271 |
T272 |
amod |
enhanced,protein |
R110 |
T272 |
T270 |
pobj |
protein,of |
R111 |
T273 |
T272 |
amod |
green,protein |
R112 |
T274 |
T272 |
amod |
fluorescent,protein |
R113 |
T275 |
T272 |
punct |
(,protein |
R114 |
T276 |
T272 |
appos |
EGFP,protein |
R115 |
T277 |
T266 |
punct |
),utility |
R116 |
T278 |
T266 |
prep |
in,utility |
R117 |
T279 |
T280 |
amod |
embryonic,stem |
R118 |
T280 |
T281 |
nmod |
stem,cells |
R119 |
T281 |
T278 |
pobj |
cells,in |
R120 |
T282 |
T280 |
punct |
(,stem |
R121 |
T283 |
T280 |
appos |
ES,stem |
R122 |
T284 |
T281 |
punct |
),cells |
R123 |
T285 |
T281 |
cc |
and,cells |
R124 |
T286 |
T281 |
conj |
mice,cells |
R125 |
T287 |
T288 |
punct |
[,2 |
R126 |
T288 |
T262 |
parataxis |
2,demonstrated |
R127 |
T289 |
T288 |
punct |
],2 |
R128 |
T290 |
T262 |
punct |
.,demonstrated |