Id |
Subject |
Object |
Predicate |
Lexical cue |
T177 |
0-10 |
sentence |
denotes |
Background |
T178 |
0-10 |
NN |
denotes |
Background |
T179 |
10-122 |
sentence |
denotes |
Non-invasive autofluorescent reporters have revolutionized lineage labeling in an array of different organisms. |
T180 |
11-23 |
JJ |
denotes |
Non-invasive |
T181 |
40-49 |
NNS |
denotes |
reporters |
T182 |
24-39 |
JJ |
denotes |
autofluorescent |
T183 |
55-69 |
VBN |
denotes |
revolutionized |
T184 |
50-54 |
VBP |
denotes |
have |
T185 |
70-77 |
NN |
denotes |
lineage |
T186 |
78-86 |
NN |
denotes |
labeling |
T187 |
87-89 |
IN |
denotes |
in |
T188 |
90-92 |
DT |
denotes |
an |
T189 |
93-98 |
NN |
denotes |
array |
T190 |
99-101 |
IN |
denotes |
of |
T191 |
102-111 |
JJ |
denotes |
different |
T192 |
112-121 |
NNS |
denotes |
organisms |
T193 |
121-122 |
. |
denotes |
. |
T194 |
122-296 |
sentence |
denotes |
In recent years green fluorescent protein (GFP) from the bioluminescent jellyfish Aequoria Victoria has gained popularity in mouse transgenic and gene targeting regimes [1]. |
T195 |
123-125 |
IN |
denotes |
In |
T196 |
227-233 |
VBN |
denotes |
gained |
T197 |
126-132 |
JJ |
denotes |
recent |
T198 |
133-138 |
NNS |
denotes |
years |
T199 |
139-144 |
JJ |
denotes |
green |
T200 |
157-164 |
NN |
denotes |
protein |
T201 |
145-156 |
JJ |
denotes |
fluorescent |
T202 |
165-166 |
-LRB- |
denotes |
( |
T203 |
166-169 |
NN |
denotes |
GFP |
T204 |
169-170 |
-RRB- |
denotes |
) |
T205 |
171-175 |
IN |
denotes |
from |
T206 |
176-179 |
DT |
denotes |
the |
T207 |
195-204 |
NN |
denotes |
jellyfish |
T208 |
180-194 |
JJ |
denotes |
bioluminescent |
T209 |
205-213 |
NNP |
denotes |
Aequoria |
T210 |
214-222 |
NNP |
denotes |
Victoria |
T211 |
223-226 |
VBZ |
denotes |
has |
T212 |
234-244 |
NN |
denotes |
popularity |
T213 |
245-247 |
IN |
denotes |
in |
T214 |
248-253 |
NN |
denotes |
mouse |
T215 |
254-264 |
JJ |
denotes |
transgenic |
T216 |
284-291 |
NNS |
denotes |
regimes |
T217 |
265-268 |
CC |
denotes |
and |
T218 |
269-273 |
NN |
denotes |
gene |
T219 |
274-283 |
VBG |
denotes |
targeting |
T220 |
292-293 |
-LRB- |
denotes |
[ |
T221 |
293-294 |
CD |
denotes |
1 |
T222 |
294-295 |
-RRB- |
denotes |
] |
T223 |
295-296 |
. |
denotes |
. |
T224 |
296-500 |
sentence |
denotes |
It offers several advantages over conventional gene-based reporters, such as lacZ and alkaline phosphatase, in that its visualization does not require a chromogenic substrate and can be realized in vivo. |
T225 |
297-299 |
PRP |
denotes |
It |
T226 |
300-306 |
VBZ |
denotes |
offers |
T227 |
307-314 |
JJ |
denotes |
several |
T228 |
315-325 |
NNS |
denotes |
advantages |
T229 |
326-330 |
IN |
denotes |
over |
T230 |
331-343 |
JJ |
denotes |
conventional |
T231 |
355-364 |
NNS |
denotes |
reporters |
T232 |
344-348 |
NN |
denotes |
gene |
T233 |
349-354 |
VBN |
denotes |
based |
T234 |
348-349 |
HYPH |
denotes |
- |
T235 |
364-366 |
, |
denotes |
, |
T236 |
366-370 |
JJ |
denotes |
such |
T237 |
371-373 |
IN |
denotes |
as |
T238 |
374-378 |
NN |
denotes |
lacZ |
T239 |
379-382 |
CC |
denotes |
and |
T240 |
383-391 |
NN |
denotes |
alkaline |
T241 |
392-403 |
NN |
denotes |
phosphatase |
T242 |
403-405 |
, |
denotes |
, |
T243 |
405-407 |
IN |
denotes |
in |
T244 |
440-447 |
VB |
denotes |
require |
T245 |
408-412 |
IN |
denotes |
that |
T246 |
413-416 |
PRP$ |
denotes |
its |
T247 |
417-430 |
NN |
denotes |
visualization |
T248 |
431-435 |
VBZ |
denotes |
does |
T249 |
436-439 |
RB |
denotes |
not |
T250 |
448-449 |
DT |
denotes |
a |
T251 |
462-471 |
NN |
denotes |
substrate |
T252 |
450-461 |
JJ |
denotes |
chromogenic |
T253 |
472-475 |
CC |
denotes |
and |
T254 |
476-479 |
MD |
denotes |
can |
T255 |
483-491 |
VBN |
denotes |
realized |
T256 |
480-482 |
VB |
denotes |
be |
T257 |
492-494 |
FW |
denotes |
in |
T258 |
495-499 |
FW |
denotes |
vivo |
T259 |
499-500 |
. |
denotes |
. |
T260 |
500-661 |
sentence |
denotes |
We have previously demonstrated the utility and developmental neutrality of enhanced green fluorescent protein (EGFP) in embryonic stem (ES) cells and mice [2]. |
T261 |
501-503 |
PRP |
denotes |
We |
T262 |
520-532 |
VBN |
denotes |
demonstrated |
T263 |
504-508 |
VBP |
denotes |
have |
T264 |
509-519 |
RB |
denotes |
previously |
T265 |
533-536 |
DT |
denotes |
the |
T266 |
537-544 |
NN |
denotes |
utility |
T267 |
545-548 |
CC |
denotes |
and |
T268 |
549-562 |
JJ |
denotes |
developmental |
T269 |
563-573 |
NN |
denotes |
neutrality |
T270 |
574-576 |
IN |
denotes |
of |
T271 |
577-585 |
VBN |
denotes |
enhanced |
T272 |
604-611 |
NN |
denotes |
protein |
T273 |
586-591 |
JJ |
denotes |
green |
T274 |
592-603 |
JJ |
denotes |
fluorescent |
T275 |
612-613 |
-LRB- |
denotes |
( |
T276 |
613-617 |
NN |
denotes |
EGFP |
T277 |
617-618 |
-RRB- |
denotes |
) |
T278 |
619-621 |
IN |
denotes |
in |
T279 |
622-631 |
JJ |
denotes |
embryonic |
T280 |
632-636 |
NN |
denotes |
stem |
T281 |
642-647 |
NNS |
denotes |
cells |
T282 |
637-638 |
-LRB- |
denotes |
( |
T283 |
638-640 |
NN |
denotes |
ES |
T284 |
640-641 |
-RRB- |
denotes |
) |
T285 |
648-651 |
CC |
denotes |
and |
T286 |
652-656 |
NNS |
denotes |
mice |
T287 |
657-658 |
-LRB- |
denotes |
[ |
T288 |
658-659 |
CD |
denotes |
2 |
T289 |
659-660 |
-RRB- |
denotes |
] |
T290 |
660-661 |
. |
denotes |
. |
T291 |
661-670 |
sentence |
denotes |
Results |
T292 |
663-670 |
NNS |
denotes |
Results |
T293 |
670-918 |
sentence |
denotes |
In this study we have used embryonic stem (ES) cell-mediated transgenesis to test the enhanced cyan fluorescent protein (ECFP) and enhanced yellow fluorescent protein (EYFP), two mutant and spectrally distinct color variants of wild type (wt) GFP. |
T294 |
671-673 |
IN |
denotes |
In |
T295 |
693-697 |
VBN |
denotes |
used |
T296 |
674-678 |
DT |
denotes |
this |
T297 |
679-684 |
NN |
denotes |
study |
T298 |
685-687 |
PRP |
denotes |
we |
T299 |
688-692 |
VBP |
denotes |
have |
T300 |
698-707 |
JJ |
denotes |
embryonic |
T301 |
708-712 |
NN |
denotes |
stem |
T302 |
718-722 |
NN |
denotes |
cell |
T303 |
713-714 |
-LRB- |
denotes |
( |
T304 |
714-716 |
NN |
denotes |
ES |
T305 |
716-717 |
-RRB- |
denotes |
) |
T306 |
723-731 |
VBN |
denotes |
mediated |
T307 |
722-723 |
HYPH |
denotes |
- |
T308 |
732-744 |
NN |
denotes |
transgenesis |
T309 |
745-747 |
TO |
denotes |
to |
T310 |
748-752 |
VB |
denotes |
test |
T311 |
753-756 |
DT |
denotes |
the |
T312 |
783-790 |
NN |
denotes |
protein |
T313 |
757-765 |
VBN |
denotes |
enhanced |
T314 |
766-770 |
NN |
denotes |
cyan |
T315 |
771-782 |
JJ |
denotes |
fluorescent |
T316 |
791-792 |
-LRB- |
denotes |
( |
T317 |
792-796 |
NN |
denotes |
ECFP |
T318 |
796-797 |
-RRB- |
denotes |
) |
T319 |
798-801 |
CC |
denotes |
and |
T320 |
802-810 |
VBN |
denotes |
enhanced |
T321 |
830-837 |
NN |
denotes |
protein |
T322 |
811-817 |
JJ |
denotes |
yellow |
T323 |
818-829 |
JJ |
denotes |
fluorescent |
T324 |
838-839 |
-LRB- |
denotes |
( |
T325 |
839-843 |
NN |
denotes |
EYFP |
T326 |
843-844 |
-RRB- |
denotes |
) |
T327 |
844-846 |
, |
denotes |
, |
T328 |
846-849 |
CD |
denotes |
two |
T329 |
887-895 |
NNS |
denotes |
variants |
T330 |
850-856 |
JJ |
denotes |
mutant |
T331 |
857-860 |
CC |
denotes |
and |
T332 |
861-871 |
RB |
denotes |
spectrally |
T333 |
872-880 |
JJ |
denotes |
distinct |
T334 |
881-886 |
NN |
denotes |
color |
T335 |
896-898 |
IN |
denotes |
of |
T336 |
899-903 |
JJ |
denotes |
wild |
T337 |
904-908 |
NN |
denotes |
type |
T338 |
914-917 |
NN |
denotes |
GFP |
T339 |
909-910 |
-LRB- |
denotes |
( |
T340 |
910-912 |
NN |
denotes |
wt |
T341 |
912-913 |
-RRB- |
denotes |
) |
T342 |
917-918 |
. |
denotes |
. |
T343 |
918-1065 |
sentence |
denotes |
We have also tested DsRed1, the novel red fluorescent protein reporter recently cloned from the Discostoma coral by virtue of its homology to GFP. |
T344 |
919-921 |
PRP |
denotes |
We |
T345 |
932-938 |
VBN |
denotes |
tested |
T346 |
922-926 |
VBP |
denotes |
have |
T347 |
927-931 |
RB |
denotes |
also |
T348 |
939-945 |
NN |
denotes |
DsRed1 |
T349 |
945-947 |
, |
denotes |
, |
T350 |
947-950 |
DT |
denotes |
the |
T351 |
981-989 |
NN |
denotes |
reporter |
T352 |
951-956 |
JJ |
denotes |
novel |
T353 |
957-960 |
JJ |
denotes |
red |
T354 |
973-980 |
NN |
denotes |
protein |
T355 |
961-972 |
JJ |
denotes |
fluorescent |
T356 |
990-998 |
RB |
denotes |
recently |
T357 |
999-1005 |
VBN |
denotes |
cloned |
T358 |
1006-1010 |
IN |
denotes |
from |
T359 |
1011-1014 |
DT |
denotes |
the |
T360 |
1026-1031 |
NN |
denotes |
coral |
T361 |
1015-1025 |
NN |
denotes |
Discostoma |
T362 |
1032-1034 |
IN |
denotes |
by |
T363 |
1035-1041 |
NN |
denotes |
virtue |
T364 |
1042-1044 |
IN |
denotes |
of |
T365 |
1045-1048 |
PRP$ |
denotes |
its |
T366 |
1049-1057 |
NN |
denotes |
homology |
T367 |
1058-1060 |
IN |
denotes |
to |
T368 |
1061-1064 |
NN |
denotes |
GFP |
T369 |
1064-1065 |
. |
denotes |
. |
T370 |
1065-1233 |
sentence |
denotes |
To this end, we have established lines of ES cells together with viable and fertile mice having widespread expression of either the ECFP or EYFP GFP-variant reporters. |
T371 |
1066-1068 |
IN |
denotes |
To |
T372 |
1087-1098 |
VBN |
denotes |
established |
T373 |
1069-1073 |
DT |
denotes |
this |
T374 |
1074-1077 |
NN |
denotes |
end |
T375 |
1077-1079 |
, |
denotes |
, |
T376 |
1079-1081 |
PRP |
denotes |
we |
T377 |
1082-1086 |
VBP |
denotes |
have |
T378 |
1099-1104 |
NNS |
denotes |
lines |
T379 |
1105-1107 |
IN |
denotes |
of |
T380 |
1108-1110 |
NN |
denotes |
ES |
T381 |
1111-1116 |
NNS |
denotes |
cells |
T382 |
1117-1125 |
RB |
denotes |
together |
T383 |
1126-1130 |
IN |
denotes |
with |
T384 |
1131-1137 |
JJ |
denotes |
viable |
T385 |
1150-1154 |
NNS |
denotes |
mice |
T386 |
1138-1141 |
CC |
denotes |
and |
T387 |
1142-1149 |
JJ |
denotes |
fertile |
T388 |
1155-1161 |
VBG |
denotes |
having |
T389 |
1162-1172 |
JJ |
denotes |
widespread |
T390 |
1173-1183 |
NN |
denotes |
expression |
T391 |
1184-1186 |
IN |
denotes |
of |
T392 |
1187-1193 |
CC |
denotes |
either |
T393 |
1223-1232 |
NNS |
denotes |
reporters |
T394 |
1194-1197 |
DT |
denotes |
the |
T395 |
1198-1202 |
NN |
denotes |
ECFP |
T396 |
1203-1205 |
CC |
denotes |
or |
T397 |
1206-1210 |
NN |
denotes |
EYFP |
T398 |
1211-1214 |
NN |
denotes |
GFP |
T399 |
1215-1222 |
NN |
denotes |
variant |
T400 |
1214-1215 |
HYPH |
denotes |
- |
T401 |
1232-1233 |
. |
denotes |
. |
T402 |
1233-1413 |
sentence |
denotes |
However, we were unable to generate equivalent DsRed1 lines, suggesting that DsRed1 is not developmentally neutral or that transgene expression cannot be sustained constitutively. |
T403 |
1234-1241 |
RB |
denotes |
However |
T404 |
1246-1250 |
VBD |
denotes |
were |
T405 |
1241-1243 |
, |
denotes |
, |
T406 |
1243-1245 |
PRP |
denotes |
we |
T407 |
1251-1257 |
JJ |
denotes |
unable |
T408 |
1258-1260 |
TO |
denotes |
to |
T409 |
1261-1269 |
VB |
denotes |
generate |
T410 |
1270-1280 |
JJ |
denotes |
equivalent |
T411 |
1288-1293 |
NNS |
denotes |
lines |
T412 |
1281-1287 |
NN |
denotes |
DsRed1 |
T413 |
1293-1295 |
, |
denotes |
, |
T414 |
1295-1305 |
VBG |
denotes |
suggesting |
T415 |
1306-1310 |
IN |
denotes |
that |
T416 |
1318-1320 |
VBZ |
denotes |
is |
T417 |
1311-1317 |
NN |
denotes |
DsRed1 |
T418 |
1321-1324 |
RB |
denotes |
not |
T419 |
1325-1340 |
RB |
denotes |
developmentally |
T420 |
1341-1348 |
JJ |
denotes |
neutral |
T421 |
1349-1351 |
CC |
denotes |
or |
T422 |
1352-1356 |
IN |
denotes |
that |
T423 |
1388-1397 |
VBN |
denotes |
sustained |
T424 |
1357-1366 |
NN |
denotes |
transgene |
T425 |
1367-1377 |
NN |
denotes |
expression |
T426 |
1378-1381 |
MD |
denotes |
can |
T427 |
1381-1384 |
RB |
denotes |
not |
T428 |
1385-1387 |
VB |
denotes |
be |
T429 |
1398-1412 |
RB |
denotes |
constitutively |
T430 |
1412-1413 |
. |
denotes |
. |
T431 |
1413-1681 |
sentence |
denotes |
Balanced (diploid <-> diploid) and polarized (tetraploid <-> diploid) chimeras comprising combinations of the ECFP and EYFP ES cells and/or embryos, demonstrate that populations of cells expressing each individual reporter can be distinguished within a single animal. |
T432 |
1414-1422 |
JJ |
denotes |
Balanced |
T433 |
1484-1492 |
NNS |
denotes |
chimeras |
T434 |
1423-1424 |
-LRB- |
denotes |
( |
T435 |
1436-1443 |
JJ |
denotes |
diploid |
T436 |
1424-1431 |
JJ |
denotes |
diploid |
T437 |
1432-1433 |
SYM |
denotes |
< |
T438 |
1433-1434 |
HYPH |
denotes |
- |
T439 |
1434-1435 |
SYM |
denotes |
> |
T440 |
1443-1444 |
-RRB- |
denotes |
) |
T441 |
1445-1448 |
CC |
denotes |
and |
T442 |
1449-1458 |
JJ |
denotes |
polarized |
T443 |
1459-1460 |
-LRB- |
denotes |
( |
T444 |
1475-1482 |
JJ |
denotes |
diploid |
T445 |
1460-1470 |
JJ |
denotes |
tetraploid |
T446 |
1471-1472 |
SYM |
denotes |
< |
T447 |
1472-1473 |
HYPH |
denotes |
- |
T448 |
1473-1474 |
SYM |
denotes |
> |
T449 |
1482-1483 |
-RRB- |
denotes |
) |
T450 |
1563-1574 |
VBP |
denotes |
demonstrate |
T451 |
1493-1503 |
VBG |
denotes |
comprising |
T452 |
1504-1516 |
NNS |
denotes |
combinations |
T453 |
1517-1519 |
IN |
denotes |
of |
T454 |
1520-1523 |
DT |
denotes |
the |
T455 |
1524-1528 |
NN |
denotes |
ECFP |
T456 |
1529-1532 |
CC |
denotes |
and |
T457 |
1533-1537 |
NN |
denotes |
EYFP |
T458 |
1538-1540 |
NN |
denotes |
ES |
T459 |
1541-1546 |
NNS |
denotes |
cells |
T460 |
1547-1550 |
CC |
denotes |
and |
T461 |
1550-1551 |
HYPH |
denotes |
/ |
T462 |
1551-1553 |
CC |
denotes |
or |
T463 |
1554-1561 |
NNS |
denotes |
embryos |
T464 |
1561-1563 |
, |
denotes |
, |
T465 |
1575-1579 |
IN |
denotes |
that |
T466 |
1644-1657 |
VBN |
denotes |
distinguished |
T467 |
1580-1591 |
NNS |
denotes |
populations |
T468 |
1592-1594 |
IN |
denotes |
of |
T469 |
1595-1600 |
NNS |
denotes |
cells |
T470 |
1601-1611 |
VBG |
denotes |
expressing |
T471 |
1612-1616 |
DT |
denotes |
each |
T472 |
1628-1636 |
NN |
denotes |
reporter |
T473 |
1617-1627 |
JJ |
denotes |
individual |
T474 |
1637-1640 |
MD |
denotes |
can |
T475 |
1641-1643 |
VB |
denotes |
be |
T476 |
1658-1664 |
IN |
denotes |
within |
T477 |
1665-1666 |
DT |
denotes |
a |
T478 |
1674-1680 |
NN |
denotes |
animal |
T479 |
1667-1673 |
JJ |
denotes |
single |
T480 |
1680-1681 |
. |
denotes |
. |
T481 |
1681-1694 |
sentence |
denotes |
Conclusions |
T482 |
1683-1694 |
NNS |
denotes |
Conclusions |
T483 |
1694-1798 |
sentence |
denotes |
GFP variant reporters are unique in allowing non-invasive multi-spectral visualization in live samples. |
T484 |
1695-1698 |
NN |
denotes |
GFP |
T485 |
1707-1716 |
NNS |
denotes |
reporters |
T486 |
1699-1706 |
JJ |
denotes |
variant |
T487 |
1717-1720 |
VBP |
denotes |
are |
T488 |
1721-1727 |
JJ |
denotes |
unique |
T489 |
1728-1730 |
IN |
denotes |
in |
T490 |
1731-1739 |
VBG |
denotes |
allowing |
T491 |
1740-1752 |
JJ |
denotes |
non-invasive |
T492 |
1768-1781 |
NN |
denotes |
visualization |
T493 |
1753-1767 |
JJ |
denotes |
multi-spectral |
T494 |
1782-1784 |
IN |
denotes |
in |
T495 |
1785-1789 |
JJ |
denotes |
live |
T496 |
1790-1797 |
NNS |
denotes |
samples |
T497 |
1797-1798 |
. |
denotes |
. |
T498 |
1798-2006 |
sentence |
denotes |
The ECFP and EYFP-expressing transgenic ES cells and mice that we have generated provide sources of cells and tissues for combinatorial, double-tagged recombination experiments, chimeras or transplantations. |
T499 |
1799-1802 |
DT |
denotes |
The |
T500 |
1842-1847 |
NNS |
denotes |
cells |
T501 |
1803-1807 |
NN |
denotes |
ECFP |
T502 |
1817-1827 |
VBG |
denotes |
expressing |
T503 |
1808-1811 |
CC |
denotes |
and |
T504 |
1812-1816 |
NN |
denotes |
EYFP |
T505 |
1816-1817 |
HYPH |
denotes |
- |
T506 |
1828-1838 |
JJ |
denotes |
transgenic |
T507 |
1839-1841 |
NN |
denotes |
ES |
T508 |
1880-1887 |
VBP |
denotes |
provide |
T509 |
1848-1851 |
CC |
denotes |
and |
T510 |
1852-1856 |
NNS |
denotes |
mice |
T511 |
1857-1861 |
WDT |
denotes |
that |
T512 |
1870-1879 |
VBN |
denotes |
generated |
T513 |
1862-1864 |
PRP |
denotes |
we |
T514 |
1865-1869 |
VBP |
denotes |
have |
T515 |
1888-1895 |
NNS |
denotes |
sources |
T516 |
1896-1898 |
IN |
denotes |
of |
T517 |
1899-1904 |
NNS |
denotes |
cells |
T518 |
1905-1908 |
CC |
denotes |
and |
T519 |
1909-1916 |
NNS |
denotes |
tissues |
T520 |
1917-1920 |
IN |
denotes |
for |
T521 |
1921-1934 |
JJ |
denotes |
combinatorial |
T522 |
1964-1975 |
NNS |
denotes |
experiments |
T523 |
1934-1936 |
, |
denotes |
, |
T524 |
1936-1942 |
JJ |
denotes |
double |
T525 |
1943-1949 |
JJ |
denotes |
tagged |
T526 |
1942-1943 |
HYPH |
denotes |
- |
T527 |
1950-1963 |
NN |
denotes |
recombination |
T528 |
1975-1977 |
, |
denotes |
, |
T529 |
1977-1985 |
NNS |
denotes |
chimeras |
T530 |
1986-1988 |
CC |
denotes |
or |
T531 |
1989-2005 |
NNS |
denotes |
transplantations |
T532 |
2005-2006 |
. |
denotes |
. |
R25 |
T180 |
T181 |
amod |
Non-invasive,reporters |
R26 |
T181 |
T183 |
nsubj |
reporters,revolutionized |
R27 |
T182 |
T181 |
amod |
autofluorescent,reporters |
R28 |
T184 |
T183 |
aux |
have,revolutionized |
R29 |
T185 |
T186 |
compound |
lineage,labeling |
R30 |
T186 |
T183 |
dobj |
labeling,revolutionized |
R31 |
T187 |
T183 |
prep |
in,revolutionized |
R32 |
T188 |
T189 |
det |
an,array |
R33 |
T189 |
T187 |
pobj |
array,in |
R34 |
T190 |
T189 |
prep |
of,array |
R35 |
T191 |
T192 |
amod |
different,organisms |
R36 |
T192 |
T190 |
pobj |
organisms,of |
R37 |
T193 |
T183 |
punct |
.,revolutionized |
R38 |
T195 |
T196 |
prep |
In,gained |
R39 |
T197 |
T198 |
amod |
recent,years |
R40 |
T198 |
T195 |
pobj |
years,In |
R41 |
T199 |
T200 |
amod |
green,protein |
R42 |
T200 |
T196 |
nsubj |
protein,gained |
R43 |
T201 |
T200 |
amod |
fluorescent,protein |
R44 |
T202 |
T200 |
punct |
(,protein |
R45 |
T203 |
T200 |
appos |
GFP,protein |
R46 |
T204 |
T200 |
punct |
),protein |
R47 |
T205 |
T200 |
prep |
from,protein |
R48 |
T206 |
T207 |
det |
the,jellyfish |
R49 |
T207 |
T205 |
pobj |
jellyfish,from |
R50 |
T208 |
T207 |
amod |
bioluminescent,jellyfish |
R51 |
T209 |
T210 |
compound |
Aequoria,Victoria |
R52 |
T210 |
T207 |
appos |
Victoria,jellyfish |
R53 |
T211 |
T196 |
aux |
has,gained |
R54 |
T212 |
T196 |
dobj |
popularity,gained |
R55 |
T213 |
T196 |
prep |
in,gained |
R56 |
T214 |
T215 |
npadvmod |
mouse,transgenic |
R57 |
T215 |
T216 |
amod |
transgenic,regimes |
R58 |
T216 |
T213 |
pobj |
regimes,in |
R59 |
T217 |
T215 |
cc |
and,transgenic |
R60 |
T218 |
T219 |
npadvmod |
gene,targeting |
R61 |
T219 |
T215 |
conj |
targeting,transgenic |
R62 |
T220 |
T221 |
punct |
[,1 |
R63 |
T221 |
T196 |
parataxis |
1,gained |
R64 |
T222 |
T221 |
punct |
],1 |
R65 |
T223 |
T196 |
punct |
.,gained |
R66 |
T225 |
T226 |
nsubj |
It,offers |
R67 |
T227 |
T228 |
amod |
several,advantages |
R68 |
T228 |
T226 |
dobj |
advantages,offers |
R69 |
T229 |
T228 |
prep |
over,advantages |
R70 |
T230 |
T231 |
amod |
conventional,reporters |
R71 |
T231 |
T229 |
pobj |
reporters,over |
R72 |
T232 |
T233 |
npadvmod |
gene,based |
R73 |
T233 |
T231 |
amod |
based,reporters |
R74 |
T234 |
T233 |
punct |
-,based |
R75 |
T235 |
T228 |
punct |
", ",advantages |
R76 |
T236 |
T237 |
amod |
such,as |
R77 |
T237 |
T228 |
prep |
as,advantages |
R78 |
T238 |
T237 |
pobj |
lacZ,as |
R79 |
T239 |
T238 |
cc |
and,lacZ |
R80 |
T240 |
T241 |
compound |
alkaline,phosphatase |
R81 |
T241 |
T238 |
conj |
phosphatase,lacZ |
R82 |
T242 |
T226 |
punct |
", ",offers |
R83 |
T243 |
T244 |
mark |
in,require |
R84 |
T244 |
T226 |
advcl |
require,offers |
R85 |
T245 |
T244 |
mark |
that,require |
R86 |
T246 |
T247 |
poss |
its,visualization |
R87 |
T247 |
T244 |
nsubj |
visualization,require |
R88 |
T248 |
T244 |
aux |
does,require |
R89 |
T249 |
T244 |
neg |
not,require |
R90 |
T250 |
T251 |
det |
a,substrate |
R91 |
T251 |
T244 |
dobj |
substrate,require |
R92 |
T252 |
T251 |
amod |
chromogenic,substrate |
R93 |
T253 |
T244 |
cc |
and,require |
R94 |
T254 |
T255 |
aux |
can,realized |
R95 |
T255 |
T244 |
conj |
realized,require |
R96 |
T256 |
T255 |
auxpass |
be,realized |
R97 |
T257 |
T258 |
advmod |
in,vivo |
R98 |
T258 |
T255 |
advmod |
vivo,realized |
R99 |
T259 |
T226 |
punct |
.,offers |
R100 |
T261 |
T262 |
nsubj |
We,demonstrated |
R101 |
T263 |
T262 |
aux |
have,demonstrated |
R102 |
T264 |
T262 |
advmod |
previously,demonstrated |
R103 |
T265 |
T266 |
det |
the,utility |
R104 |
T266 |
T262 |
dobj |
utility,demonstrated |
R105 |
T267 |
T266 |
cc |
and,utility |
R106 |
T268 |
T269 |
amod |
developmental,neutrality |
R107 |
T269 |
T266 |
conj |
neutrality,utility |
R108 |
T270 |
T266 |
prep |
of,utility |
R109 |
T271 |
T272 |
amod |
enhanced,protein |
R110 |
T272 |
T270 |
pobj |
protein,of |
R111 |
T273 |
T272 |
amod |
green,protein |
R112 |
T274 |
T272 |
amod |
fluorescent,protein |
R113 |
T275 |
T272 |
punct |
(,protein |
R114 |
T276 |
T272 |
appos |
EGFP,protein |
R115 |
T277 |
T266 |
punct |
),utility |
R116 |
T278 |
T266 |
prep |
in,utility |
R117 |
T279 |
T280 |
amod |
embryonic,stem |
R118 |
T280 |
T281 |
nmod |
stem,cells |
R119 |
T281 |
T278 |
pobj |
cells,in |
R120 |
T282 |
T280 |
punct |
(,stem |
R121 |
T283 |
T280 |
appos |
ES,stem |
R122 |
T284 |
T281 |
punct |
),cells |
R123 |
T285 |
T281 |
cc |
and,cells |
R124 |
T286 |
T281 |
conj |
mice,cells |
R125 |
T287 |
T288 |
punct |
[,2 |
R126 |
T288 |
T262 |
parataxis |
2,demonstrated |
R127 |
T289 |
T288 |
punct |
],2 |
R128 |
T290 |
T262 |
punct |
.,demonstrated |
R129 |
T294 |
T295 |
prep |
In,used |
R130 |
T296 |
T297 |
det |
this,study |
R131 |
T297 |
T294 |
pobj |
study,In |
R132 |
T298 |
T295 |
nsubj |
we,used |
R133 |
T299 |
T295 |
aux |
have,used |
R134 |
T300 |
T301 |
amod |
embryonic,stem |
R135 |
T301 |
T302 |
nmod |
stem,cell |
R136 |
T302 |
T306 |
npadvmod |
cell,mediated |
R137 |
T303 |
T301 |
punct |
(,stem |
R138 |
T304 |
T301 |
appos |
ES,stem |
R139 |
T305 |
T302 |
punct |
),cell |
R140 |
T306 |
T308 |
amod |
mediated,transgenesis |
R141 |
T307 |
T306 |
punct |
-,mediated |
R142 |
T308 |
T295 |
dobj |
transgenesis,used |
R143 |
T309 |
T310 |
aux |
to,test |
R144 |
T310 |
T295 |
advcl |
test,used |
R145 |
T311 |
T312 |
det |
the,protein |
R146 |
T312 |
T310 |
dobj |
protein,test |
R147 |
T313 |
T312 |
amod |
enhanced,protein |
R148 |
T314 |
T312 |
nmod |
cyan,protein |
R149 |
T315 |
T312 |
amod |
fluorescent,protein |
R150 |
T316 |
T312 |
punct |
(,protein |
R151 |
T317 |
T312 |
appos |
ECFP,protein |
R152 |
T318 |
T312 |
punct |
),protein |
R153 |
T319 |
T312 |
cc |
and,protein |
R154 |
T320 |
T321 |
amod |
enhanced,protein |
R155 |
T321 |
T312 |
conj |
protein,protein |
R156 |
T322 |
T321 |
amod |
yellow,protein |
R157 |
T323 |
T321 |
amod |
fluorescent,protein |
R158 |
T324 |
T321 |
punct |
(,protein |
R159 |
T325 |
T321 |
appos |
EYFP,protein |
R160 |
T326 |
T312 |
punct |
),protein |
R161 |
T327 |
T312 |
punct |
", ",protein |
R162 |
T328 |
T329 |
nummod |
two,variants |
R163 |
T329 |
T312 |
appos |
variants,protein |
R164 |
T330 |
T329 |
amod |
mutant,variants |
R165 |
T331 |
T330 |
cc |
and,mutant |
R166 |
T332 |
T333 |
advmod |
spectrally,distinct |
R167 |
T333 |
T330 |
conj |
distinct,mutant |
R168 |
T334 |
T329 |
compound |
color,variants |
R169 |
T335 |
T329 |
prep |
of,variants |
R170 |
T336 |
T337 |
amod |
wild,type |
R171 |
T337 |
T338 |
nmod |
type,GFP |
R172 |
T338 |
T335 |
pobj |
GFP,of |
R173 |
T339 |
T337 |
punct |
(,type |
R174 |
T340 |
T337 |
appos |
wt,type |
R175 |
T341 |
T338 |
punct |
),GFP |
R176 |
T342 |
T295 |
punct |
.,used |
R177 |
T344 |
T345 |
nsubj |
We,tested |
R178 |
T346 |
T345 |
aux |
have,tested |
R179 |
T347 |
T345 |
advmod |
also,tested |
R180 |
T348 |
T345 |
dobj |
DsRed1,tested |
R181 |
T349 |
T348 |
punct |
", ",DsRed1 |
R182 |
T350 |
T351 |
det |
the,reporter |
R183 |
T351 |
T348 |
appos |
reporter,DsRed1 |
R184 |
T352 |
T351 |
amod |
novel,reporter |
R185 |
T353 |
T354 |
amod |
red,protein |
R186 |
T354 |
T351 |
compound |
protein,reporter |
R187 |
T355 |
T354 |
amod |
fluorescent,protein |
R188 |
T356 |
T357 |
advmod |
recently,cloned |
R189 |
T357 |
T351 |
acl |
cloned,reporter |
R190 |
T358 |
T357 |
prep |
from,cloned |
R191 |
T359 |
T360 |
det |
the,coral |
R192 |
T360 |
T358 |
pobj |
coral,from |
R193 |
T361 |
T360 |
compound |
Discostoma,coral |
R194 |
T362 |
T357 |
prep |
by,cloned |
R195 |
T363 |
T362 |
pobj |
virtue,by |
R196 |
T364 |
T363 |
prep |
of,virtue |
R197 |
T365 |
T366 |
poss |
its,homology |
R198 |
T366 |
T364 |
pobj |
homology,of |
R199 |
T367 |
T366 |
prep |
to,homology |
R200 |
T368 |
T367 |
pobj |
GFP,to |
R201 |
T369 |
T345 |
punct |
.,tested |
R202 |
T371 |
T372 |
prep |
To,established |
R203 |
T373 |
T374 |
det |
this,end |
R204 |
T374 |
T371 |
pobj |
end,To |
R205 |
T375 |
T372 |
punct |
", ",established |
R206 |
T376 |
T372 |
nsubj |
we,established |
R207 |
T377 |
T372 |
aux |
have,established |
R208 |
T378 |
T372 |
dobj |
lines,established |
R209 |
T379 |
T378 |
prep |
of,lines |
R210 |
T380 |
T381 |
compound |
ES,cells |
R211 |
T381 |
T379 |
pobj |
cells,of |
R212 |
T382 |
T383 |
advmod |
together,with |
R213 |
T383 |
T372 |
prep |
with,established |
R214 |
T384 |
T385 |
amod |
viable,mice |
R215 |
T385 |
T383 |
pobj |
mice,with |
R216 |
T386 |
T384 |
cc |
and,viable |
R217 |
T387 |
T384 |
conj |
fertile,viable |
R218 |
T388 |
T385 |
acl |
having,mice |
R219 |
T389 |
T390 |
amod |
widespread,expression |
R220 |
T390 |
T388 |
dobj |
expression,having |
R221 |
T391 |
T390 |
prep |
of,expression |
R222 |
T392 |
T393 |
preconj |
either,reporters |
R224 |
T394 |
T393 |
det |
the,reporters |
R225 |
T395 |
T393 |
nmod |
ECFP,reporters |
R226 |
T396 |
T395 |
cc |
or,ECFP |
R227 |
T397 |
T395 |
conj |
EYFP,ECFP |
R228 |
T398 |
T399 |
compound |
GFP,variant |
R229 |
T399 |
T393 |
compound |
variant,reporters |
R230 |
T400 |
T399 |
punct |
-,variant |
R231 |
T401 |
T372 |
punct |
.,established |
R232 |
T403 |
T404 |
advmod |
However,were |
R233 |
T405 |
T404 |
punct |
", ",were |
R234 |
T406 |
T404 |
nsubj |
we,were |
R235 |
T407 |
T404 |
acomp |
unable,were |
R236 |
T408 |
T409 |
aux |
to,generate |
R237 |
T409 |
T407 |
xcomp |
generate,unable |
R238 |
T410 |
T411 |
amod |
equivalent,lines |
R239 |
T411 |
T409 |
dobj |
lines,generate |
R240 |
T412 |
T411 |
compound |
DsRed1,lines |
R241 |
T413 |
T404 |
punct |
", ",were |
R242 |
T414 |
T404 |
advcl |
suggesting,were |
R243 |
T415 |
T416 |
mark |
that,is |
R244 |
T416 |
T414 |
advcl |
is,suggesting |
R245 |
T417 |
T416 |
nsubj |
DsRed1,is |
R246 |
T418 |
T416 |
neg |
not,is |
R247 |
T419 |
T420 |
advmod |
developmentally,neutral |
R248 |
T420 |
T416 |
acomp |
neutral,is |
R249 |
T421 |
T416 |
cc |
or,is |
R250 |
T422 |
T423 |
mark |
that,sustained |
R251 |
T423 |
T416 |
conj |
sustained,is |
R252 |
T424 |
T425 |
compound |
transgene,expression |
R253 |
T425 |
T423 |
nsubjpass |
expression,sustained |
R254 |
T426 |
T423 |
aux |
can,sustained |
R255 |
T427 |
T423 |
neg |
not,sustained |
R256 |
T428 |
T423 |
auxpass |
be,sustained |
R257 |
T429 |
T423 |
advmod |
constitutively,sustained |
R258 |
T430 |
T404 |
punct |
.,were |
R259 |
T432 |
T433 |
amod |
Balanced,chimeras |
R260 |
T433 |
T450 |
nsubj |
chimeras,demonstrate |
R261 |
T434 |
T435 |
punct |
(,diploid |
R262 |
T435 |
T432 |
parataxis |
diploid,Balanced |
R263 |
T436 |
T435 |
nsubj |
diploid,diploid |
R264 |
T437 |
T435 |
punct |
<,diploid |
R265 |
T438 |
T435 |
punct |
-,diploid |
R266 |
T439 |
T435 |
punct |
>,diploid |
R267 |
T440 |
T435 |
punct |
),diploid |
R268 |
T441 |
T432 |
cc |
and,Balanced |
R269 |
T442 |
T432 |
conj |
polarized,Balanced |
R270 |
T443 |
T444 |
punct |
(,diploid |
R271 |
T444 |
T442 |
parataxis |
diploid,polarized |
R272 |
T445 |
T444 |
nsubj |
tetraploid,diploid |
R273 |
T446 |
T444 |
punct |
<,diploid |
R274 |
T447 |
T444 |
punct |
-,diploid |
R275 |
T448 |
T444 |
punct |
>,diploid |
R276 |
T449 |
T444 |
punct |
),diploid |
R277 |
T451 |
T433 |
acl |
comprising,chimeras |
R278 |
T452 |
T451 |
dobj |
combinations,comprising |
R279 |
T453 |
T452 |
prep |
of,combinations |
R280 |
T454 |
T455 |
det |
the,ECFP |
R281 |
T455 |
T453 |
pobj |
ECFP,of |
R282 |
T456 |
T455 |
cc |
and,ECFP |
R283 |
T457 |
T455 |
conj |
EYFP,ECFP |
R284 |
T458 |
T459 |
compound |
ES,cells |
R285 |
T459 |
T455 |
appos |
cells,ECFP |
R286 |
T460 |
T459 |
cc |
and,cells |
R287 |
T461 |
T460 |
punct |
/,and |
R288 |
T462 |
T460 |
cc |
or,and |
R289 |
T463 |
T459 |
conj |
embryos,cells |
R290 |
T464 |
T450 |
punct |
", ",demonstrate |
R291 |
T465 |
T466 |
mark |
that,distinguished |
R292 |
T466 |
T450 |
ccomp |
distinguished,demonstrate |
R293 |
T467 |
T466 |
nsubjpass |
populations,distinguished |
R294 |
T468 |
T467 |
prep |
of,populations |
R295 |
T469 |
T468 |
pobj |
cells,of |
R296 |
T470 |
T467 |
acl |
expressing,populations |
R297 |
T471 |
T472 |
det |
each,reporter |
R298 |
T472 |
T470 |
dobj |
reporter,expressing |
R299 |
T473 |
T472 |
amod |
individual,reporter |
R300 |
T474 |
T466 |
aux |
can,distinguished |
R301 |
T475 |
T466 |
auxpass |
be,distinguished |
R302 |
T476 |
T466 |
prep |
within,distinguished |
R303 |
T477 |
T478 |
det |
a,animal |
R304 |
T478 |
T476 |
pobj |
animal,within |
R305 |
T479 |
T478 |
amod |
single,animal |
R306 |
T480 |
T450 |
punct |
.,demonstrate |
R307 |
T484 |
T485 |
nmod |
GFP,reporters |
R308 |
T485 |
T487 |
nsubj |
reporters,are |
R309 |
T486 |
T484 |
amod |
variant,GFP |
R310 |
T488 |
T487 |
acomp |
unique,are |
R311 |
T489 |
T488 |
prep |
in,unique |
R312 |
T490 |
T489 |
pcomp |
allowing,in |
R313 |
T491 |
T492 |
amod |
non-invasive,visualization |
R314 |
T492 |
T490 |
dobj |
visualization,allowing |
R315 |
T493 |
T492 |
amod |
multi-spectral,visualization |
R316 |
T494 |
T490 |
prep |
in,allowing |
R317 |
T495 |
T496 |
amod |
live,samples |
R318 |
T496 |
T494 |
pobj |
samples,in |
R319 |
T497 |
T487 |
punct |
.,are |
R320 |
T499 |
T500 |
det |
The,cells |
R322 |
T501 |
T502 |
npadvmod |
ECFP,expressing |
R323 |
T502 |
T500 |
amod |
expressing,cells |
R324 |
T503 |
T501 |
cc |
and,ECFP |
R325 |
T504 |
T501 |
conj |
EYFP,ECFP |
R326 |
T505 |
T502 |
punct |
-,expressing |
R327 |
T506 |
T500 |
amod |
transgenic,cells |
R328 |
T507 |
T500 |
compound |
ES,cells |
R329 |
T509 |
T500 |
cc |
and,cells |
R330 |
T510 |
T500 |
conj |
mice,cells |
R331 |
T511 |
T512 |
dep |
that,generated |
R332 |
T512 |
T500 |
relcl |
generated,cells |
R333 |
T513 |
T512 |
nsubj |
we,generated |
R334 |
T514 |
T512 |
aux |
have,generated |
R335 |
T515 |
T508 |
dobj |
sources,provide |
R336 |
T516 |
T515 |
prep |
of,sources |
R337 |
T517 |
T516 |
pobj |
cells,of |
R338 |
T518 |
T517 |
cc |
and,cells |
R339 |
T519 |
T517 |
conj |
tissues,cells |
R340 |
T520 |
T508 |
prep |
for,provide |
R341 |
T521 |
T522 |
amod |
combinatorial,experiments |
R342 |
T522 |
T520 |
pobj |
experiments,for |
R343 |
T523 |
T522 |
punct |
", ",experiments |
R344 |
T524 |
T525 |
amod |
double,tagged |
R345 |
T525 |
T522 |
amod |
tagged,experiments |
R346 |
T526 |
T525 |
punct |
-,tagged |
R347 |
T527 |
T522 |
compound |
recombination,experiments |
R348 |
T528 |
T522 |
punct |
", ",experiments |
R349 |
T529 |
T522 |
conj |
chimeras,experiments |
R350 |
T530 |
T529 |
cc |
or,chimeras |
R351 |
T531 |
T529 |
conj |
transplantations,chimeras |
R352 |
T532 |
T508 |
punct |
.,provide |
R223 |
T393 |
T391 |
pobj |
reporters,of |
R321 |
T500 |
T508 |
nsubj |
cells,provide |