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PMC:1142324 / 23228-24003 JSONTXT

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Id Subject Object Predicate Lexical cue
T6757 292-294 IN denotes as
T6692 0-3 NN denotes RNA
T6693 12-25 NN denotes hybridisation
T6694 4-6 FW denotes in
T6695 7-11 FW denotes situ
T6696 25-114 sentence denotes In situ hybridisation on frozen sections were carried out using 35S-labelled RNA probes.
T6697 26-28 FW denotes In
T6698 29-33 FW denotes situ
T6699 34-47 NN denotes hybridisation
T6700 72-79 VBN denotes carried
T6701 48-50 IN denotes on
T6702 51-57 VBN denotes frozen
T6703 58-66 NNS denotes sections
T6704 67-71 VBD denotes were
T6705 80-83 RP denotes out
T6706 84-89 VBG denotes using
T6707 90-93 CD denotes 35S
T6708 94-102 VBN denotes labelled
T6709 93-94 HYPH denotes -
T6710 107-113 NNS denotes probes
T6711 103-106 NN denotes RNA
T6712 113-114 . denotes .
T6713 114-270 sentence denotes Briefly, 610 bp of mouse ADAM22 cDNA (position: 1351–1960 from initiating ATG) was cloned into the pBluescript II SK(+) or the pBluescript II KS(+) vector.
T6714 115-122 RB denotes Briefly
T6715 198-204 VBN denotes cloned
T6716 122-124 , denotes ,
T6717 124-127 CD denotes 610
T6718 128-130 NN denotes bp
T6719 131-133 IN denotes of
T6720 134-139 NN denotes mouse
T6721 147-151 NN denotes cDNA
T6722 140-146 NN denotes ADAM22
T6723 152-153 -LRB- denotes (
T6724 168-172 CD denotes 1960
T6725 153-161 NN denotes position
T6726 161-163 : denotes :
T6727 163-167 CD denotes 1351
T6728 167-168 SYM denotes –
T6729 173-177 IN denotes from
T6730 178-188 VBG denotes initiating
T6731 189-192 NN denotes ATG
T6732 192-193 -RRB- denotes )
T6733 194-197 VBD denotes was
T6734 205-209 IN denotes into
T6735 210-213 DT denotes the
T6736 229-231 NN denotes SK
T6737 214-225 NNP denotes pBluescript
T6738 226-228 CD denotes II
T6739 231-232 -LRB- denotes (
T6740 232-233 SYM denotes +
T6741 233-234 -RRB- denotes )
T6742 235-237 CC denotes or
T6743 238-241 DT denotes the
T6744 257-259 NN denotes KS
T6745 242-253 NNP denotes pBluescript
T6746 254-256 CD denotes II
T6747 263-269 NN denotes vector
T6748 259-260 -LRB- denotes (
T6749 260-261 SYM denotes +
T6750 261-262 -RRB- denotes )
T6751 269-270 . denotes .
T6752 270-396 sentence denotes Using these plasmids as templates, sense and antisense labelled RNA probes were generated by T7 RNA polymerase and [α35S]UTP.
T6753 271-276 VBG denotes Using
T6754 351-360 VBN denotes generated
T6755 277-282 DT denotes these
T6756 283-291 NNS denotes plasmids
T6758 295-304 NNS denotes templates
T6759 304-306 , denotes ,
T6760 306-311 NN denotes sense
T6761 326-334 VBN denotes labelled
T6762 312-315 CC denotes and
T6763 316-325 JJ denotes antisense
T6764 339-345 NNS denotes probes
T6765 335-338 NN denotes RNA
T6766 346-350 VBD denotes were
T6767 361-363 IN denotes by
T6768 364-366 NN denotes T7
T6769 371-381 NN denotes polymerase
T6770 367-370 NN denotes RNA
T6771 382-385 CC denotes and
T6772 386-387 -LRB- denotes [
T6773 392-395 NN denotes UTP
T6774 387-391 NN denotes α35S
T6775 391-392 -RRB- denotes ]
T6776 395-396 . denotes .
T6777 396-490 sentence denotes Frozen brain and spinal cord from 2 month-old C57BL/6 female mice were used in this analysis.
T6778 397-403 VBN denotes Frozen
T6779 404-409 NN denotes brain
T6780 468-472 VBN denotes used
T6781 410-413 CC denotes and
T6782 414-420 JJ denotes spinal
T6783 421-425 NN denotes cord
T6784 426-430 IN denotes from
T6785 431-432 CD denotes 2
T6786 433-438 NN denotes month
T6787 439-442 JJ denotes old
T6788 438-439 HYPH denotes -
T6789 458-462 NNS denotes mice
T6790 443-448 NN denotes C57BL
T6791 448-449 HYPH denotes /
T6792 449-450 CD denotes 6
T6793 451-457 JJ denotes female
T6794 463-467 VBD denotes were
T6795 473-475 IN denotes in
T6796 476-480 DT denotes this
T6797 481-489 NN denotes analysis
T6798 489-490 . denotes .
T6799 490-620 sentence denotes Pretreatment, hybridisation, RNase treatment and washing was carried out following the protocol described in the literature [33].
T6800 491-503 NN denotes Pretreatment
T6801 552-559 VBN denotes carried
T6802 503-505 , denotes ,
T6803 505-518 NN denotes hybridisation
T6804 518-520 , denotes ,
T6805 520-525 NN denotes RNase
T6806 526-535 NN denotes treatment
T6807 536-539 CC denotes and
T6808 540-547 NN denotes washing
T6809 548-551 VBD denotes was
T6810 560-563 RP denotes out
T6811 564-573 VBG denotes following
T6812 574-577 DT denotes the
T6813 578-586 NN denotes protocol
T6814 587-596 VBN denotes described
T6815 597-599 IN denotes in
T6816 600-603 DT denotes the
T6817 604-614 NN denotes literature
T6818 615-616 -LRB- denotes [
T6819 616-618 CD denotes 33
T6820 618-619 -RRB- denotes ]
T6821 619-620 . denotes .
T6822 620-775 sentence denotes Dehydrated slides were attached to imaging plates for 48 hours and the autoradiograms were analysed using a Bio-Image Analyser (BAS3000, Fuji Photo Film).
T6823 621-631 VBN denotes Dehydrated
T6824 632-638 NNS denotes slides
T6825 644-652 VBN denotes attached
T6826 639-643 VBD denotes were
T6827 653-655 IN denotes to
T6828 656-663 NN denotes imaging
T6829 664-670 NNS denotes plates
T6830 671-674 IN denotes for
T6831 675-677 CD denotes 48
T6832 678-683 NNS denotes hours
T6833 684-687 CC denotes and
T6834 688-691 DT denotes the
T6835 692-706 NNS denotes autoradiograms
T6836 712-720 VBN denotes analysed
T6837 707-711 VBD denotes were
T6838 721-726 VBG denotes using
T6839 727-728 DT denotes a
T6840 739-747 NNP denotes Analyser
T6841 729-738 NNP denotes Bio-Image
T6842 748-749 -LRB- denotes (
T6843 769-773 NNP denotes Film
T6844 749-756 NN denotes BAS3000
T6845 756-758 , denotes ,
T6846 758-762 NNP denotes Fuji
T6847 763-768 NNP denotes Photo
T6848 773-774 -RRB- denotes )
T6849 774-775 . denotes .
R4241 T6692 T6693 nmod RNA,hybridisation
R4242 T6694 T6695 advmod in,situ
R4243 T6695 T6693 amod situ,hybridisation
R4244 T6697 T6698 advmod In,situ
R4245 T6698 T6699 amod situ,hybridisation
R4246 T6699 T6700 nsubjpass hybridisation,carried
R4247 T6701 T6699 prep on,hybridisation
R4248 T6702 T6703 amod frozen,sections
R4249 T6703 T6701 pobj sections,on
R4250 T6704 T6700 auxpass were,carried
R4251 T6705 T6700 prt out,carried
R4252 T6706 T6700 advcl using,carried
R4253 T6707 T6708 advmod 35S,labelled
R4254 T6708 T6710 amod labelled,probes
R4255 T6709 T6708 punct -,labelled
R4256 T6710 T6706 dobj probes,using
R4257 T6711 T6710 compound RNA,probes
R4258 T6712 T6700 punct .,carried
R4259 T6714 T6715 advmod Briefly,cloned
R4260 T6716 T6715 punct ", ",cloned
R4261 T6717 T6718 nummod 610,bp
R4262 T6718 T6715 nsubjpass bp,cloned
R4263 T6719 T6718 prep of,bp
R4264 T6720 T6721 compound mouse,cDNA
R4265 T6721 T6719 pobj cDNA,of
R4266 T6722 T6721 compound ADAM22,cDNA
R4267 T6723 T6724 punct (,1960
R4268 T6724 T6721 parataxis 1960,cDNA
R4269 T6725 T6724 dep position,1960
R4270 T6726 T6724 punct : ,1960
R4271 T6727 T6724 nummod 1351,1960
R4272 T6728 T6724 punct –,1960
R4273 T6729 T6724 prep from,1960
R4274 T6730 T6731 amod initiating,ATG
R4275 T6731 T6729 pobj ATG,from
R4276 T6732 T6724 punct ),1960
R4277 T6733 T6715 auxpass was,cloned
R4278 T6734 T6715 prep into,cloned
R4279 T6735 T6736 det the,SK
R4280 T6736 T6734 pobj SK,into
R4281 T6737 T6736 nmod pBluescript,SK
R4282 T6738 T6736 nummod II,SK
R4283 T6739 T6740 punct (,+
R4284 T6740 T6736 punct +,SK
R4285 T6741 T6740 punct ),+
R4286 T6742 T6736 cc or,SK
R4287 T6743 T6744 det the,KS
R4288 T6744 T6747 nmod KS,vector
R4289 T6745 T6744 nmod pBluescript,KS
R4290 T6746 T6744 nummod II,KS
R4291 T6747 T6736 conj vector,SK
R4292 T6748 T6749 punct (,+
R4293 T6749 T6744 punct +,KS
R4294 T6750 T6749 punct ),+
R4295 T6751 T6715 punct .,cloned
R4296 T6753 T6754 advcl Using,generated
R4297 T6755 T6756 det these,plasmids
R4298 T6756 T6753 dobj plasmids,Using
R4299 T6757 T6753 prep as,Using
R4300 T6758 T6757 pobj templates,as
R4301 T6759 T6754 punct ", ",generated
R4302 T6760 T6761 npadvmod sense,labelled
R4303 T6761 T6764 amod labelled,probes
R4304 T6762 T6760 cc and,sense
R4305 T6763 T6760 conj antisense,sense
R4306 T6764 T6754 nsubjpass probes,generated
R4307 T6765 T6764 compound RNA,probes
R4308 T6766 T6754 auxpass were,generated
R4309 T6767 T6754 agent by,generated
R4310 T6768 T6769 compound T7,polymerase
R4311 T6769 T6767 pobj polymerase,by
R4312 T6770 T6769 compound RNA,polymerase
R4313 T6771 T6769 cc and,polymerase
R4314 T6772 T6773 punct [,UTP
R4315 T6773 T6769 conj UTP,polymerase
R4316 T6774 T6773 nmod α35S,UTP
R4317 T6775 T6773 punct ],UTP
R4318 T6776 T6754 punct .,generated
R4319 T6778 T6779 amod Frozen,brain
R4320 T6779 T6780 nsubjpass brain,used
R4321 T6781 T6779 cc and,brain
R4322 T6782 T6783 amod spinal,cord
R4323 T6783 T6779 conj cord,brain
R4324 T6784 T6779 prep from,brain
R4325 T6785 T6786 nummod 2,month
R4326 T6786 T6787 npadvmod month,old
R4327 T6787 T6789 amod old,mice
R4328 T6788 T6787 punct -,old
R4329 T6789 T6784 pobj mice,from
R4330 T6790 T6789 nmod C57BL,mice
R4331 T6791 T6790 punct /,C57BL
R4332 T6792 T6790 nummod 6,C57BL
R4333 T6793 T6789 amod female,mice
R4334 T6794 T6780 auxpass were,used
R4335 T6795 T6780 prep in,used
R4336 T6796 T6797 det this,analysis
R4337 T6797 T6795 pobj analysis,in
R4338 T6798 T6780 punct .,used
R4339 T6800 T6801 nsubjpass Pretreatment,carried
R4340 T6802 T6800 punct ", ",Pretreatment
R4341 T6803 T6800 conj hybridisation,Pretreatment
R4342 T6804 T6803 punct ", ",hybridisation
R4343 T6805 T6806 compound RNase,treatment
R4344 T6806 T6803 conj treatment,hybridisation
R4345 T6807 T6806 cc and,treatment
R4346 T6808 T6806 conj washing,treatment
R4347 T6809 T6801 auxpass was,carried
R4348 T6810 T6801 prt out,carried
R4349 T6811 T6801 advcl following,carried
R4350 T6812 T6813 det the,protocol
R4351 T6813 T6811 dobj protocol,following
R4352 T6814 T6813 acl described,protocol
R4353 T6815 T6814 prep in,described
R4354 T6816 T6817 det the,literature
R4355 T6817 T6815 pobj literature,in
R4356 T6818 T6819 punct [,33
R4357 T6819 T6801 parataxis 33,carried
R4358 T6820 T6819 punct ],33
R4359 T6821 T6801 punct .,carried
R4360 T6823 T6824 amod Dehydrated,slides
R4361 T6824 T6825 nsubjpass slides,attached
R4362 T6826 T6825 auxpass were,attached
R4363 T6827 T6825 prep to,attached
R4364 T6828 T6829 compound imaging,plates
R4365 T6829 T6827 pobj plates,to
R4366 T6830 T6825 prep for,attached
R4367 T6831 T6832 nummod 48,hours
R4368 T6832 T6830 pobj hours,for
R4369 T6833 T6825 cc and,attached
R4370 T6834 T6835 det the,autoradiograms
R4371 T6835 T6836 nsubjpass autoradiograms,analysed
R4372 T6836 T6825 conj analysed,attached
R4373 T6837 T6836 auxpass were,analysed
R4374 T6838 T6836 advcl using,analysed
R4375 T6839 T6840 det a,Analyser
R4376 T6840 T6838 dobj Analyser,using
R4377 T6841 T6840 compound Bio-Image,Analyser
R4378 T6842 T6843 punct (,Film
R4379 T6843 T6840 parataxis Film,Analyser
R4380 T6844 T6843 dep BAS3000,Film
R4381 T6845 T6843 punct ", ",Film
R4382 T6846 T6843 compound Fuji,Film
R4383 T6847 T6843 compound Photo,Film
R4384 T6848 T6843 punct ),Film
R4385 T6849 T6836 punct .,analysed

craft-ca-core-ex-dev

Below, discontinuous spans are shown in the bag model. You can change it to the chain model.

Id Subject Object Predicate Lexical cue
T6663 0-3 CHEBI_SO_EXT:RNA denotes RNA
T6664 12-25 GO:0097617 denotes hybridisation
T6665 34-47 GO:0097617 denotes hybridisation
T6666 90-93 CHEBI:37983 denotes 35S
T6667 94-102 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes labelled
T6668 103-113 CHEBI_SO_EXT:riboprobe denotes RNA probes
T6669 128-130 SO_EXT:0000028 denotes bp
T6670 134-139 NCBITaxon:10088 denotes mouse
T6671 140-146 PR_EXT:000003718 denotes ADAM22
T6672 147-151 SO_EXT:cDNA denotes cDNA
T6673 178-192 SO_EXT:0000318 denotes initiating ATG
T6674 198-204 SO_EXT:sequence_cloning_process denotes cloned
T6675 263-269 SO_EXT:0000440 denotes vector
T6676 283-291 SO_EXT:0000155 denotes plasmids
T6677 316-325 SO:0000077 denotes antisense
T6678 326-334 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes labelled
T6679 335-345 CHEBI_SO_EXT:riboprobe denotes RNA probes
T6680 364-366 NCBITaxon:10760 denotes T7
T6681 364-381 PR_EXT:P00573 denotes T7 RNA polymerase
T6682 367-370 CHEBI_SO_EXT:RNA denotes RNA
T6683 367-381 GO_EXT:0034062 denotes RNA polymerase
T6684 388-391 CHEBI:37983 denotes 35S
T6685 392-395 CHEBI_EXT:UTP denotes UTP
T6686 404-409 UBERON:0000955 denotes brain
T6687 414-425 UBERON:0002240 denotes spinal cord
T6688 451-457 PATO_UBERON_EXT:female_or_bearer_of_femaleness denotes female
T6689 458-462 NCBITaxon:10088 denotes mice
T6690 505-518 GO:0097617 denotes hybridisation
T6691 520-525 GO_EXT:0004540 denotes RNase

craft-ca-core-dev

Below, discontinuous spans are shown in the bag model. You can change it to the chain model.

Id Subject Object Predicate Lexical cue
T6646 12-25 GO:0097617 denotes hybridisation
T6647 34-47 GO:0097617 denotes hybridisation
T6648 90-93 CHEBI:37983 denotes 35S
T6649 128-130 SO:0000028 denotes bp
T6650 134-139 NCBITaxon:10088 denotes mouse
T6651 140-146 PR:000003718 denotes ADAM22
T6652 178-192 SO:0000318 denotes initiating ATG
T6653 263-269 SO:0000440 denotes vector
T6654 283-291 SO:0000155 denotes plasmids
T6655 316-325 SO:0000077 denotes antisense
T6656 364-366 NCBITaxon:10760 denotes T7
T6657 364-381 PR:P00573 denotes T7 RNA polymerase
T6658 388-391 CHEBI:37983 denotes 35S
T6659 404-409 UBERON:0000955 denotes brain
T6660 414-425 UBERON:0002240 denotes spinal cord
T6661 458-462 NCBITaxon:10088 denotes mice
T6662 505-518 GO:0097617 denotes hybridisation