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Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T27 11-27 UBERON:0001021 denotes peripheral nerve
T28 47-53 PR:000003718 denotes ADAM22
T29 64-68 NCBITaxon:10088 denotes mice
T30 90-96 PR:000003718 denotes ADAM22
T31 121-125 SO:0000704 denotes gene
T32 158-167 GO:0010467 denotes expressed
T33 180-195 UBERON:0001016 denotes nervous systems
T34 213-219 PR:000003718 denotes ADAM22
T35 339-345 GO:0031012 denotes matrix
T36 404-410 PR:000003718 denotes ADAM22
T37 424-428 SO:0000704 denotes gene
T38 451-457 PR:000003718 denotes ADAM22
T39 467-471 NCBITaxon:10088 denotes mice
T40 482-488 PR:000003718 denotes ADAM22
T41 499-503 NCBITaxon:10088 denotes mice
T42 587-592 GO:0007567 denotes birth
T43 658-662 GO:0016265 denotes died
T44 765-780 UBERON:0000956 denotes cerebral cortex
T45 784-794 UBERON:0002037 denotes cerebellum
T46 861-878 UBERON:0001021 denotes peripheral nerves
T47 947-953 PR:000003718 denotes ADAM22
T48 1008-1022 UBERON:0001016 denotes nervous system
T49 1044-1050 PR:000003718 denotes ADAM22
T50 1105-1110 NCBITaxon:9606 denotes human
T400 1242-1250 GO:0016020 denotes membrane
T401 1266-1272 SO:0000417 denotes domain
T402 1318-1324 SO:0000417 denotes domain
T403 1348-1354 SO:0000417 denotes domain
T404 1410-1417 NCBITaxon:40674 denotes mammals
T405 1466-1479 GO:0009566 denotes fertilization
T406 1496-1508 GO:0022008 denotes neurogenesis
T407 1512-1523 GO:0006508 denotes proteolysis
T408 1612-1625 GO:0005576 denotes extracellular
T409 1626-1633 SO:0000417 denotes domains
T410 1637-1645 GO:0016020 denotes membrane
T411 1700-1706 PR:000001279 denotes ADAM17
T412 1708-1712 PR:000001279 denotes TACE
T413 1732-1738 MOP:0000780 denotes cleave
T414 1769-1797 PR:000000134 denotes tumour necrosis factor alpha
T415 1804-1811 CHEBI:28304 denotes heparin
T416 1804-1862 PR:000008459 denotes heparin-binding epidermal growth factor-like growth factor
T417 1820-1829 UBERON:0007376 denotes epidermal
T418 1873-1905 PR:000016285 denotes transforming growth factor alpha
T419 1922-1928 PR:000001279 denotes ADAM17
T420 1934-1938 NCBITaxon:10088 denotes mice
T421 1958-1964 PR:000001279 denotes ADAM17
T422 1980-1993 GO:0009790 denotes embryogenesis
T423 2106-2112 GO:0031012 denotes matrix
T424 2295-2300 PR:000003715 denotes ADAM2
T425 2310-2315 PR:000003732 denotes ADAM3
T426 2358-2369 CL:0000019 denotes spermatozoa
T427 2375-2378 CL:0000025 denotes egg
T428 2411-2416 PR:000003715 denotes ADAM2
T429 2421-2426 PR:000003732 denotes ADAM3
T430 2520-2526 SO:0000417 denotes domain
T431 2664-2670 PR:000003710 denotes ADAM11
T432 2672-2678 PR:000003718 denotes ADAM22
T433 2683-2689 PR:000003719 denotes ADAM23
T434 2690-2695 SO:0000704 denotes genes
T435 2717-2727 GO:0010467 denotes expression
T436 2735-2740 NCBITaxon:9606 denotes human
T437 2745-2751 NCBITaxon:39107 denotes murine
T438 2752-2767 UBERON:0001016 denotes nervous systems
T439 2905-2911 PR:000003719 denotes ADAM23
T440 2940-2952 GO:0009986 denotes cell surface
T441 2974-2997 GO:0034683 denotes alpha-v beta-3 integrin
T442 2982-2988 PR:000002042 denotes beta-3
T443 3037-3043 PR:000003719 denotes ADAM23
T444 3044-3048 SO:0000704 denotes gene
T445 3056-3061 NCBITaxon:10088 denotes mouse
T446 3083-3088 GO:0016265 denotes death
T447 3151-3156 GO:0016265 denotes death
T448 3165-3170 NCBITaxon:10088 denotes mouse
T449 3232-3238 GO:0031012 denotes matrix
T450 3259-3273 UBERON:0001016 denotes nervous system
T451 3292-3298 PR:000003719 denotes ADAM23
T452 3319-3325 PR:000003718 denotes ADAM22
T453 3330-3336 PR:000003719 denotes ADAM23
T454 3350-3360 SO:0000857 denotes homologous
T455 3380-3393 GO:0005576 denotes extracellular
T456 3394-3401 SO:0000417 denotes domains
T457 3542-3548 SO:0000417 denotes domain
T458 3592-3598 PR:000003718 denotes ADAM22
T459 3656-3670 UBERON:0001016 denotes nervous system
T460 3680-3686 PR:000003719 denotes ADAM23
T461 3732-3738 PR:000003718 denotes ADAM22
T462 3766-3772 PR:000003718 denotes Adam22
T463 3773-3777 SO:0000704 denotes gene
T464 3787-3791 NCBITaxon:10088 denotes mice
T1145 3817-3823 PR:000003718 denotes ADAM22
T1146 3834-3838 NCBITaxon:10088 denotes mice
T1147 3839-3843 NCBITaxon:10088 denotes Mice
T1148 3882-3888 PR:000003718 denotes Adam22
T1149 3889-3893 SO:0000704 denotes gene
T1150 4035-4052 SO:0000319 denotes termination codon
T1151 4071-4075 SO:0000147 denotes exon
T1152 4085-4096 SO:0001062 denotes pro-protein
T1153 4097-4103 SO:0000417 denotes domain
T1154 4136-4142 PR:000003718 denotes ADAM22
T1155 4191-4197 SO:0001023 denotes allele
T1156 4209-4220 SO:0001062 denotes pro-protein
T1157 4221-4227 SO:0000417 denotes domain
T1158 4292-4297 PR:000003137 denotes Furin
T1159 4399-4405 PR:000003718 denotes ADAM22
T1160 4449-4455 PR:000003718 denotes ADAM22
T1161 4544-4552 GO:0042571 denotes antibody
T1162 4575-4586 GO:0005737 denotes cytoplasmic
T1163 4587-4596 _FRAGMENT denotes domain of
T1164 4608-4615 SO:0000417 denotes protein
T1165 4601-4607 PR:000003718 denotes ADAM22
T1166 4678-4683 GO:0007567 denotes birth
T1167 4784-4789 GO:0007567 denotes natal
T1168 4938-4943 UBERON:0002101 denotes limbs
T1169 5018-5021 GO:0016265 denotes die
T1170 5514-5520 PR:000003718 denotes ADAM22
T1171 5542-5555 GO:0009790 denotes embryogenesis
T1172 5711-5715 GO:0016265 denotes died
T1173 5758-5762 GO:0016265 denotes died
T1174 5865-5870 GO:0007567 denotes birth
T1755 6013-6019 PR:000003718 denotes ADAM22
T1756 6030-6034 NCBITaxon:10088 denotes mice
T1757 6068-6078 GO:0010467 denotes expression
T1758 6082-6088 PR:000003718 denotes ADAM22
T1759 6101-6106 NCBITaxon:9606 denotes human
T1760 6111-6116 NCBITaxon:10088 denotes mouse
T1761 6117-6122 UBERON:0000955 denotes brain
T1762 6191-6197 PR:000003718 denotes ADAM22
T1763 6198-6208 SO:0000673 denotes transcript
T1764 6216-6221 UBERON:0007023 denotes adult
T1765 6222-6227 NCBITaxon:10088 denotes mouse
T1766 6228-6231 UBERON:0001017 denotes CNS
T1767 6251-6264 GO:0097617 denotes hybridisation
T1768 6294-6297 CHEBI:37983 denotes 35S
T1769 6337-6343 PR:000003718 denotes ADAM22
T1770 6353-6362 GO:0010467 denotes expressed
T1771 6378-6383 UBERON:0007023 denotes adult
T1772 6384-6389 NCBITaxon:10088 denotes mouse
T1773 6390-6393 UBERON:0001017 denotes CNS
T1774 6429-6439 UBERON:0002037 denotes cerebellar
T1775 6429-6453 CL:0001031 denotes cerebellar granule cells
T1776 6458-6479 UBERON:0002421 denotes hippocampal formation
T1777 6488-6499 UBERON:0002240 denotes spinal cord
T1778 6501-6514 GO:0097617 denotes hybridisation
T1779 6544-6555 UBERON:0002020 denotes grey matter
T1780 6577-6583 PR:000003718 denotes ADAM22
T1781 6584-6595 SO:0000673 denotes transcripts
T1782 6603-6606 UBERON:0001017 denotes CNS
T1783 6638-6644 PR:000003710 denotes ADAM11
T1784 6652-6660 CL:0000540 denotes neuronal
T1785 6661-6671 GO:0010467 denotes expression
T1786 6727-6733 PR:000003718 denotes ADAM22
T1787 6739-6749 GO:0010467 denotes expression
T1788 6753-6761 CL:0000540 denotes neuronal
T1789 6769-6774 NCBITaxon:10088 denotes mouse
T1790 6775-6778 UBERON:0001017 denotes CNS
T1791 6873-6883 UBERON:0002037 denotes cerebellum
T1792 6939-6949 GO:0010467 denotes expression
T1793 6953-6959 PR:000003718 denotes ADAM22
T1794 6973-6983 UBERON:0002037 denotes cerebellar
T1795 6973-6997 CL:0001031 denotes cerebellar granule cells
T1796 6999-7011 CL:0000120 denotes granule cell
T1797 7042-7055 CL:0000121 denotes Purkinje cell
T1798 7068-7077 PR:000004967 denotes calbindin
T1799 7102-7107 NCBITaxon:10088 denotes mouse
T1800 7136-7157 UBERON:0002421 denotes Hippocampal formation
T1801 7224-7237 CL:0000540 denotes neuronal cell
T1802 7224-7247 GO:0001764 denotes neuronal cell migration
T1803 7279-7284 NCBITaxon:10088 denotes mouse
T1804 7315-7318 PR:000010228 denotes MBP
T1805 7320-7326 UBERON:0000345 denotes myelin
T1806 7320-7340 PR:000010228 denotes myelin basic protein
T1807 7327-7332 CHEBI:22695 denotes basic
T1808 7372-7382 UBERON:0002037 denotes cerebellum
T1809 7397-7408 UBERON:0002240 denotes spinal cord
T1810 7561-7566 NCBITaxon:10088 denotes mouse
T1811 7567-7572 UBERON:0000955 denotes brain
T1812 7621-7632 UBERON:0002240 denotes spinal cord
T1813 7637-7654 UBERON:0001021 denotes peripheral nerves
T1814 7725-7731 UBERON:0000345 denotes myelin
T1815 7765-7771 UBERON:0000345 denotes myelin
T1816 7780-7786 UBERON:0000345 denotes myelin
T1817 7807-7821 UBERON:0001322 denotes sciatic nerves
T1818 7836-7853 UBERON:0001645 denotes trigeminal nerves
T1819 7927-7938 UBERON:0002240 denotes spinal cord
T1820 7972-7984 CL:0002573 denotes Schwann cell
T1821 8040-8044 NCBITaxon:10088 denotes mice
T1822 8070-8095 CL:0000218 denotes myelinating Schwann cells
T1823 8100-8105 GO:0030424 denotes axons
T1824 8107-8115 CHEBI:10545 denotes electron
T1825 8149-8162 UBERON:0001322 denotes sciatic nerve
T1826 8187-8200 CL:0002573 denotes Schwann cells
T1827 8219-8225 UBERON:0000345 denotes myelin
T1828 8288-8292 NCBITaxon:10088 denotes mice
T1829 8350-8355 GO:0030424 denotes axons
T1830 8424-8437 UBERON:0001322 denotes sciatic nerve
T1831 8470-8476 GO:0005634 denotes nuclei
T1832 8511-8514 PR:000010228 denotes MBP
T1833 8571-8583 CL:0002573 denotes Schwann cell
T1834 8717-8733 _FRAGMENT denotes proliferation of
T1835 8738-8751 GO:0014010 denotes Schwann cells
T1836 8738-8751 CL:0002573 denotes Schwann cells
T1837 8816-8822 PR:000003718 denotes ADAM22
T1838 8833-8837 NCBITaxon:10088 denotes mice
T2556 8846-8852 PR:000003718 denotes ADAM22
T2557 8853-8863 SO:0000673 denotes transcript
T2558 8891-8916 UBERON:0000010 denotes peripheral nervous system
T2559 8932-8937 NCBITaxon:9606 denotes human
T2560 8938-8944 PR:000003718 denotes ADAM22
T2561 8964-8972 GO:0008380 denotes splicing
T2562 9015-9020 SO:0000147 denotes exons
T2563 9084-9088 SO:0000147 denotes exon
T2564 9090-9094 SO:0000147 denotes exon
T2565 9106-9111 NCBITaxon:10088 denotes mouse
T2566 9177-9182 NCBITaxon:10088 denotes mouse
T2567 9183-9189 PR:000003718 denotes ADAM22
T2568 9190-9201 SO:0000673 denotes transcripts
T2569 9227-9232 NCBITaxon:10088 denotes mouse
T2570 9233-9239 SO:0001026 denotes genome
T2571 9249-9257 SO:0000857 denotes homology
T2572 9297-9302 NCBITaxon:10088 denotes mouse
T2573 9303-9309 SO:0001026 denotes genome
T2574 9310-9316 SO:0000149 denotes contig
T2575 9349-9354 NCBITaxon:10088 denotes mouse
T2576 9355-9361 PR:000003718 denotes Adam22
T2577 9362-9366 SO:0000704 denotes gene
T2578 9376-9382 SO:0000149 denotes contig
T2579 9411-9416 SO:0000147 denotes exons
T2580 9466-9471 NCBITaxon:9606 denotes human
T2581 9472-9478 PR:000003718 denotes ADAM22
T2582 9479-9486 SO:0001060 denotes isoform
T2583 9536-9547 SO:0000673 denotes transcripts
T2584 9561-9568 SO:0000112 denotes primers
T2585 9585-9589 SO:0000147 denotes exon
T2586 9620-9625 NCBITaxon:10088 denotes mouse
T2587 9626-9636 UBERON:0002037 denotes cerebellum
T2588 9757-9764 SO:0001060 denotes isoform
T2589 9793-9800 SO:0001060 denotes isoform
T2590 9843-9847 SO:0000147 denotes exon
T2591 9856-9861 SO:0000147 denotes exons
T2592 9939-9944 UBERON:0007023 denotes adult
T2593 9945-9950 NCBITaxon:10088 denotes mouse
T2594 9951-9958 UBERON:0000479 denotes tissues
T2595 9972-9983 UBERON:0002240 denotes spinal cord
T2596 9985-10005 UBERON:0000044 denotes dorsal root ganglion
T2597 10007-10010 UBERON:0000044 denotes DRG
T2598 10013-10026 UBERON:0001322 denotes sciatic nerve
T2599 10031-10047 _FRAGMENT denotes cultured primary
T2600 10056-10061 CL:0000001 denotes cells
T2601 10048-10061 CL:0002573 denotes Schwann cells
T2602 10126-10132 UBERON:0000479 denotes tissue
T2603 10263-10276 UBERON:0001322 denotes sciatic nerve
T2604 10299-10307 _FRAGMENT denotes cultured
T2605 10316-10321 CL:0000010 denotes cells
T2606 10308-10321 CL:0002573 denotes Schwann cells
T2607 10393-10400 UBERON:0000479 denotes tissues
T2608 10440-10443 UBERON:0000044 denotes DRG
T2609 10528-10532 SO:0000147 denotes exon
T2610 10563-10574 SO:0000673 denotes transcripts
T2611 10706-10713 SO:0001026 denotes genomic
T2612 10757-10762 SO:0000147 denotes exons
T2613 10764-10769 SO:0000147 denotes exons
T2614 10828-10833 SO:0000147 denotes exons
T2615 10839-10846 SO:0000357 denotes flanked
T2616 10863-10870 SO:0000188 denotes introns
T2617 10897-10901 SO:0000147 denotes Exon
T2618 10977-10983 PR:000003718 denotes ADAM22
T2619 10984-10995 SO:0000673 denotes transcripts
T2620 11040-11045 SO:0000147 denotes exons
T2621 11084-11089 SO:0000147 denotes exons
T2622 11174-11179 SO:0000147 denotes exons
T2623 11211-11224 SO:0000717 denotes reading frame
T2624 11343-11349 PR:000003718 denotes ADAM22
T2625 11350-11361 SO:0000673 denotes transcripts
T2626 11399-11402 UBERON:0001017 denotes CNS
T2627 11420-11424 SO:0000147 denotes exon
T2628 11430-11433 UBERON:0000044 denotes DRG
T2629 11451-11455 SO:0000147 denotes exon
T2630 11465-11477 CL:0002573 denotes Schwann-cell
T2631 11493-11497 SO:0000147 denotes exon
T2632 11548-11554 PR:000003718 denotes ADAM22
T2633 11558-11567 GO:0010467 denotes expressed
T2634 11653-11656 UBERON:0000010 denotes PNS
T3453 11720-11726 PR:000003718 denotes ADAM22
T3454 11741-11745 NCBITaxon:10088 denotes mice
T3455 11762-11768 PR:000003718 denotes Adam22
T3456 11769-11773 SO:0000704 denotes gene
T3457 11775-11781 PR:000003718 denotes ADAM22
T3458 11792-11796 NCBITaxon:10088 denotes mice
T3459 11835-11840 GO:0016265 denotes death
T3460 11972-11977 GO:0016265 denotes death
T3461 12176-12180 NCBITaxon:10088 denotes mice
T3462 12220-12224 GO:0016265 denotes died
T3463 12259-12267 UBERON:0001851 denotes cortical
T3464 12302-12307 UBERON:0000955 denotes brain
T3465 12302-12319 GO:0007420 denotes brain development
T3466 12363-12367 NCBITaxon:10088 denotes mice
T3467 12380-12386 CL:0000540 denotes neuron
T3468 12396-12417 PR:000005259 denotes activator of cyclin 5
T3469 12419-12422 PR:000005259 denotes p35
T3470 12454-12462 CL:0000540 denotes neuronal
T3471 12454-12472 GO:0001764 denotes neuronal migration
T3472 12494-12500 PR:000003718 denotes ADAM22
T3473 12512-12521 GO:0010467 denotes expressed
T3474 12529-12541 GO:0009986 denotes cell surface
T3475 12592-12598 GO:0031012 denotes matrix
T3476 12647-12653 PR:000003718 denotes ADAM22
T3477 12678-12686 CL:0000540 denotes neuronal
T3478 12678-12696 GO:0001764 denotes neuronal migration
T3479 12823-12828 NCBITaxon:10088 denotes mouse
T3480 12829-12834 UBERON:0000955 denotes brain
T3481 12867-12873 PR:000003718 denotes ADAM22
T3482 12877-12885 CL:0000540 denotes neuronal
T3483 12877-12895 GO:0001764 denotes neuronal migration
T3484 12948-12953 GO:0016265 denotes death
T3485 12974-12978 UBERON:0000104 denotes life
T3486 13015-13039 UBERON:0002410 denotes autonomic nervous system
T3487 13053-13059 UBERON:0001021 denotes nerves
T3488 13066-13073 GO:0002087 denotes control
T3489 13125-13143 UBERON:0001004 denotes respiration system
T3490 13206-13211 GO:0007567 denotes birth
T3491 13230-13236 UBERON:0000345 denotes myelin
T3492 13252-13255 NCBITaxon:10114 denotes rat
T3493 13295-13314 PR:000012879 denotes proteolipid protein
T3494 13316-13319 PR:000012879 denotes PLP
T3495 13321-13325 SO:0000704 denotes gene
T3496 13371-13375 GO:0016265 denotes dies
T3497 13397-13402 GO:0007567 denotes natal
T3498 13417-13420 NCBITaxon:10114 denotes rat
T3499 13428-13433 GO:0016265 denotes death
T3500 13470-13480 UBERON:0002298 denotes brain stem
T3501 13504-13536 GO:0002087 denotes autonomic control of respiration
T3502 13605-13610 GO:0016265 denotes death
T3503 13614-13620 PR:000003718 denotes ADAM22
T3504 13631-13635 NCBITaxon:10088 denotes mice
T3505 13679-13685 PR:000003718 denotes ADAM22
T3506 13696-13700 NCBITaxon:10088 denotes mice
T3507 13791-13798 _FRAGMENT denotes sciatic
T3508 13814-13820 UBERON:0001322 denotes nerves
T3509 13803-13820 UBERON:0001645 denotes trigeminal nerves
T3510 13831-13837 UBERON:0000345 denotes myelin
T3511 13855-13866 UBERON:0002240 denotes spinal cord
T3512 13875-13880 UBERON:0000955 denotes brain
T3513 13971-13988 UBERON:0001021 denotes peripheral nerves
T3514 14005-14009 NCBITaxon:10088 denotes mice
T3515 14022-14034 CL:0002573 denotes Schwann cell
T3516 14062-14075 UBERON:0001322 denotes sciatic nerve
T3517 14092-14121 CL:0000218 denotes pro-myelinating Schwann cells
T3518 14138-14141 PR:000010228 denotes MBP
T3519 14212-14240 CL:0000218 denotes pro-myelinating Schwann cell
T3520 14269-14275 UBERON:0000345 denotes myelin
T3521 14300-14306 PR:000003718 denotes ADAM22
T3522 14334-14346 CL:0002573 denotes Schwann cell
T3523 14334-14346 _FRAGMENT denotes Schwann cell
T3524 14375-14388 GO:0014010 denotes proliferation
T3525 14342-14362 GO:0030154 denotes cell differentiation
T3526 14443-14449 PR:000003718 denotes ADAM22
T3527 14460-14464 NCBITaxon:10088 denotes mice
T3528 14574-14581 SO:0000417 denotes domains
T3529 14618-14625 PR:000009131 denotes alpha-6
T3530 14618-14641 GO:0034675 denotes alpha-6 beta-1 integrin
T3531 14626-14632 PR:000002040 denotes beta-1
T3532 14649-14661 CL:0002573 denotes Schwann cell
T3533 14689-14695 UBERON:0000345 denotes myelin
T3534 14725-14734 GO:0005607 denotes laminin-2
T3535 14765-14771 UBERON:0000345 denotes myelin
T3536 14791-14800 GO:0005607 denotes laminin-2
T3537 14837-14841 NCBITaxon:10088 denotes mice
T3538 14846-14852 NCBITaxon:9606 denotes humans
T3539 14873-14880 PR:000009131 denotes alpha-6
T3540 14873-14896 GO:0034675 denotes alpha-6 beta-1 integrin
T3541 14881-14887 PR:000002040 denotes beta-1
T3542 14901-14913 PR:000006267 denotes dystroglycan
T3543 14955-14961 UBERON:0000345 denotes myelin
T3544 15017-15032 PR:000002040 denotes beta-1 integrin
T3545 15037-15052 PR:000009658 denotes laminin gamma-1
T3546 15056-15068 CL:0002573 denotes Schwann cell
T3547 15089-15098 GO:0005607 denotes laminin-2
T3548 15163-15168 GO:0030424 denotes axons
T3549 15191-15197 UBERON:0000345 denotes myelin
T3550 15256-15262 PR:000003718 denotes ADAM22
T3551 15273-15277 NCBITaxon:10088 denotes mice
T3552 15332-15337 GO:0030424 denotes axons
T3553 15355-15361 UBERON:0001021 denotes nerves
T3554 15366-15371 UBERON:0002211 denotes roots
T3555 15388-15394 PR:000003718 denotes ADAM22
T3556 15405-15414 GO:0065007 denotes modulates
T3557 15476-15482 UBERON:0000345 denotes myelin
T4447 15846-15851 SO:0000147 denotes exons
T4448 15939-15951 UBERON:0001016 denotes neurological
T4449 16040-16046 PR:000003718 denotes ADAM22
T4450 16119-16124 NCBITaxon:9606 denotes human
T4451 16125-16137 UBERON:0001016 denotes neurological
T4597 16177-16183 PR:000003718 denotes Adam22
T4598 16184-16188 SO:0000704 denotes gene
T4599 16189-16205 SO:0001644 denotes targeting vector
T4600 16216-16223 SO:0001026 denotes genomic
T4601 16247-16252 SO:0000147 denotes exons
T4602 16277-16283 PR:000003718 denotes Adam22
T4603 16284-16288 SO:0000704 denotes gene
T4604 16317-16324 SO:0001026 denotes genomic
T4605 16368-16371 CHEBI:60004 denotes mix
T4606 16396-16403 SO:0000112 denotes primers
T4607 16422-16426 SO:0000147 denotes exon
T4608 16458-16463 NCBITaxon:10088 denotes mouse
T4609 16464-16470 PR:000003718 denotes Adam22
T4610 16471-16475 SO:0000704 denotes gene
T4611 16500-16508 SO:0005853 denotes cassette
T4612 16514-16518 SO:0000147 denotes exon
T4613 16528-16534 PR:000003718 denotes Adam22
T4614 16535-16539 SO:0000704 denotes gene
T4615 16594-16601 SO:0001026 denotes genomic
T4616 16667-16675 SO:0005853 denotes cassette
T4617 16699-16707 SO:0005853 denotes cassette
T4794 16786-16792 PR:000003718 denotes Adam22
T4795 16803-16807 NCBITaxon:10088 denotes mice
T4796 16823-16839 SO:0001644 denotes targeting vector
T4797 16868-16877 UBERON:0000922 denotes embryonic
T4798 16868-16882 _FRAGMENT denotes embryonic stem
T4799 16888-16893 CL:0002322 denotes cells
T4800 16884-16886 _FRAGMENT denotes ES
T4801 16960-16967 SO:0001026 denotes genomic
T4802 17012-17026 SO:0000121 denotes forward primer
T4803 17084-17092 SO:0005853 denotes cassette
T4804 17102-17116 SO:0000132 denotes reverse primer
T4805 17173-17189 SO:0001644 denotes targeting vector
T4806 17397-17399 CL:0002322 denotes ES
T4807 17426-17436 GO:0009566 denotes fertilized
T4808 17441-17446 NCBITaxon:10088 denotes mouse
T4809 17447-17451 CL:0000025 denotes eggs
T4810 17459-17475 UBERON:0007236 denotes eight-cell stage
T4811 17510-17515 GO:0007618 denotes mated
T4812 17572-17576 NCBITaxon:10088 denotes mice
T4813 17615-17619 NCBITaxon:10088 denotes mice
T4814 17657-17661 NCBITaxon:10088 denotes mice
T5032 17726-17731 NCBITaxon:10088 denotes Mouse
T5033 17732-17739 SO:0001026 denotes genomic
T5034 17799-17804 UBERON:0002107 denotes liver
T5035 17823-17828 PR:P23940 denotes BamHI
T5036 17838-17845 SO:0001026 denotes genomic
T5037 17871-17874 CHEBI:37972 denotes 32P
T5038 17897-17902 PR:P23940 denotes BamHI
T5039 17903-17910 SO:0001026 denotes genomic
T5040 17946-17951 SO:0000147 denotes exons
T5149 17962-17970 GO:0042571 denotes Antibody
T5150 17997-18000 PR:000010228 denotes MBP
T5151 18038-18049 GO:0005737 denotes cytoplasmic
T5152 18050-18056 SO:0000417 denotes domain
T5153 18065-18070 NCBITaxon:9606 denotes human
T5154 18071-18077 PR:000003718 denotes ADAM22
T5155 18078-18085 SO:0001060 denotes isoform
T5156 18172-18178 PR:000003718 denotes ADAM22
T5157 18235-18238 CHEBI:44557 denotes NTA
T5158 18352-18360 CHEBI:60809 denotes adjuvant
T5159 18385-18392 CHEBI:60004 denotes mixture
T5160 18422-18429 NCBITaxon:9986 denotes rabbits
T5161 18439-18444 UBERON:0001977 denotes serum
T5162 18475-18481 PR:000003718 denotes ADAM22
T5163 18512-18515 PR:000010228 denotes MBP
T5164 18522-18528 PR:000003718 denotes ADAM22
T5165 18540-18547 MOP:0000779 denotes coupled
T5166 18597-18607 GO:0042571 denotes antibodies
T5167 18642-18652 GO:0042571 denotes antibodies
T5168 18751-18759 GO:0042571 denotes antibody
T5169 18766-18771 NCBITaxon:9606 denotes human
T5170 18776-18781 NCBITaxon:10088 denotes mouse
T5171 18782-18788 PR:000003718 denotes ADAM22
T5172 18865-18873 GO:0042571 denotes antibody
T5173 18927-18932 NCBITaxon:10088 denotes mouse
T5174 18933-18940 UBERON:0000479 denotes tissues
T5449 18980-18986 PR:000003718 denotes ADAM22
T5450 19002-19008 PR:000003718 denotes Adam22
T5451 19013-19017 NCBITaxon:10088 denotes mice
T5452 19071-19081 UBERON:0002037 denotes cerebellum
T5453 19108-19113 NCBITaxon:10088 denotes mouse
T5454 19178-19188 GO:0019835 denotes cell lysis
T5455 19203-19207 CHEBI:9754 denotes Tris
T5456 19208-19211 CHEBI:17883 denotes HCl
T5457 19228-19232 CHEBI:26710 denotes NaCl
T5458 19237-19242 CHEBI:63016 denotes NP-40
T5459 19380-19383 CHEBI:8984 denotes SDS
T5460 19411-19425 CHEBI:53325 denotes nitrocellulose
T5461 19480-19488 GO:0042571 denotes antibody
T5462 19501-19512 GO:0005737 denotes cytoplasmic
T5463 19513-19519 SO:0000417 denotes domain
T5464 19523-19529 PR:000003718 denotes ADAM22
T5465 19550-19560 GO:0042571 denotes antibodies
T5466 19582-19593 NCBITaxon:3704 denotes horseradish
T5467 19621-19629 GO:0042571 denotes antibody
T5666 19711-19718 _FRAGMENT denotes Primary
T5667 19727-19731 CL:0000001 denotes cell
T5668 19719-19731 CL:0002573 denotes Schwann cell
T5669 19740-19747 _FRAGMENT denotes Primary
T5670 19756-19761 CL:0000001 denotes cells
T5671 19748-19761 CL:0002573 denotes Schwann cells
T5672 19801-19805 NCBITaxon:10088 denotes mice
T5673 19877-19891 UBERON:0001322 denotes sciatic nerves
T5674 19990-19997 CHEBI:17234 denotes glucose
T5675 20076-20085 CHEBI:2682 denotes fungizone
T5676 20111-20120 CHEBI:42471 denotes forskolin
T5677 20227-20241 UBERON:0001322 denotes sciatic nerves
T5678 20586-20594 _FRAGMENT denotes cultured
T5679 20603-20608 CL:0000010 denotes cells
T5680 20595-20608 CL:0002573 denotes Schwann cells
T5925 20699-20704 UBERON:0007023 denotes Adult
T5926 20718-20722 NCBITaxon:10088 denotes mice
T5927 20777-20787 UBERON:0002037 denotes cerebellum
T5928 20789-20800 UBERON:0002240 denotes spinal cord
T5929 20802-20815 UBERON:0001322 denotes sciatic nerve
T5930 20817-20820 UBERON:0000044 denotes DRG
T5931 20825-20833 _FRAGMENT denotes cultured
T5932 20842-20847 CL:0000010 denotes cells
T5933 20834-20847 CL:0002573 denotes Schwann cells
T5934 20965-20971 SO:0000112 denotes primer
T5935 21126-21132 PR:000003718 denotes ADAM22
T5936 21142-21149 SO:0000112 denotes primers
T5937 21161-21169 GO:0008380 denotes splicing
T5938 21186-21197 GO:0005737 denotes cytoplasmic
T5939 21198-21204 SO:0000417 denotes domain
T5940 21208-21222 SO:0000121 denotes forward primer
T5941 21260-21268 GO:0016020 denotes membrane
T5942 21269-21275 SO:0000417 denotes domain
T5943 21282-21296 SO:0000132 denotes reverse primer
T5944 21324-21328 SO:0000147 denotes exon
T5945 21334-21341 SO:0000112 denotes primers
T5946 21384-21391 SO:0001030 denotes forward
T5947 21434-21441 SO:0001031 denotes reverse
T6171 21491-21495 NCBITaxon:10088 denotes Mice
T6172 21519-21530 CHEBI:35702 denotes ethyl ether
T6173 21576-21590 CHEBI:64276 denotes glutaraldehyde
T6174 21591-21599 CHEBI:75958 denotes solution
T6175 21612-21619 CHEBI:28304 denotes heparin
T6176 21651-21665 UBERON:0001322 denotes Sciatic nerves
T6177 21667-21684 UBERON:0001645 denotes trigeminal nerves
T6178 21686-21691 UBERON:0000955 denotes brain
T6179 21696-21707 UBERON:0002240 denotes spinal cord
T6180 21755-21763 CHEBI:16842 denotes formalin
T6181 21769-21780 UBERON:0002240 denotes spinal cord
T6182 21791-21796 UBERON:0001021 denotes nerve
T6183 21879-21886 CHEBI:16236 denotes ethanol
T6184 22035-22043 CHEBI:10545 denotes electron
T6185 22153-22160 CHEBI:16236 denotes ethanol
T6186 22203-22211 CHEBI:10545 denotes electron
T6187 22277-22283 NCBITaxon:33208 denotes Animal
T6376 22371-22383 CHEBI:9750 denotes Triton X-100
T6377 22449-22457 CHEBI:75958 denotes solution
T6378 22486-22496 GO:0042571 denotes antibodies
T6379 22558-22563 NCBITaxon:10088 denotes mouse
T6380 22569-22583 PR:000004967 denotes calbindin 28 K
T6381 22611-22616 NCBITaxon:10088 denotes mouse
T6382 22622-22625 PR:000010228 denotes MBP
T6383 22683-22688 NCBITaxon:10088 denotes mouse
T6384 22694-22699 PR:000006252 denotes Neu N
T6385 22719-22725 NCBITaxon:9986 denotes rabbit
T6386 22762-22768 GO:0005634 denotes Nuclei
T6387 22802-22806 CHEBI:51231 denotes DAPI
T6388 22816-22819 PR:000010228 denotes MBP
T6389 22963-22973 GO:0042571 denotes antibodies
T6390 22975-22978 CHEBI:37987 denotes Cy3
T6391 22987-22993 NCBITaxon:9793 denotes donkey
T6392 22999-23005 NCBITaxon:9986 denotes rabbit
T6393 23006-23009 GO:0071735 denotes IgG
T6394 23044-23048 CHEBI:37926 denotes FITC
T6395 23058-23064 NCBITaxon:9793 denotes donkey
T6396 23070-23075 NCBITaxon:10088 denotes mouse
T6397 23076-23079 GO:0071735 denotes IgG
T6646 23240-23253 GO:0097617 denotes hybridisation
T6647 23262-23275 GO:0097617 denotes hybridisation
T6648 23318-23321 CHEBI:37983 denotes 35S
T6649 23356-23358 SO:0000028 denotes bp
T6650 23362-23367 NCBITaxon:10088 denotes mouse
T6651 23368-23374 PR:000003718 denotes ADAM22
T6652 23406-23420 SO:0000318 denotes initiating ATG
T6653 23491-23497 SO:0000440 denotes vector
T6654 23511-23519 SO:0000155 denotes plasmids
T6655 23544-23553 SO:0000077 denotes antisense
T6656 23592-23594 NCBITaxon:10760 denotes T7
T6657 23592-23609 PR:P00573 denotes T7 RNA polymerase
T6658 23616-23619 CHEBI:37983 denotes 35S
T6659 23632-23637 UBERON:0000955 denotes brain
T6660 23642-23653 UBERON:0002240 denotes spinal cord
T6661 23686-23690 NCBITaxon:10088 denotes mice
T6662 23733-23746 GO:0097617 denotes hybridisation
T4437 15531-15537 PR:000003718 denotes ADAM22
T4438 15548-15552 NCBITaxon:10088 denotes mice
T4439 15569-15575 PR:000003718 denotes ADAM22
T4440 15612-15615 UBERON:0001017 denotes CNS
T4441 15624-15627 UBERON:0000010 denotes PNS
T4442 15668-15674 PR:000003718 denotes ADAM22
T4443 15690-15695 NCBITaxon:9606 denotes human
T4444 15753-15758 NCBITaxon:9606 denotes human
T4445 15759-15765 PR:000003718 denotes ADAM22
T4446 15766-15770 SO:0000704 denotes gene
R3015 T4799 T4798 _lexicallyChainedTo cells,embryonic stem
R3016 T4799 T4800 _lexicallyChainedTo cells,ES
R3753 T5932 T5931 _lexicallyChainedTo cells,cultured
R1057 T1835 T1834 _lexicallyChainedTo Schwann cells,proliferation of
R1564 T2600 T2599 _lexicallyChainedTo cells,cultured primary
R1565 T2605 T2604 _lexicallyChainedTo cells,cultured
R2115 T3508 T3507 _lexicallyChainedTo nerves,sciatic
R2116 T3524 T3523 _lexicallyChainedTo proliferation,Schwann cell
R3551 T5667 T5666 _lexicallyChainedTo cell,Primary
R3552 T5670 T5669 _lexicallyChainedTo cells,Primary
R3553 T5679 T5678 _lexicallyChainedTo cells,cultured
R665 T1164 T1163 _lexicallyChainedTo protein,domain of

craft-ca-core-ex-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T51 11-27 UBERON:0001021 denotes peripheral nerve
T52 32-43 GO_EXT:myelin_assembly_or_myelination denotes myelination
T53 47-53 PR_EXT:000003718 denotes ADAM22
T54 64-68 NCBITaxon:10088 denotes mice
T55 90-96 PR_EXT:000003718 denotes ADAM22
T56 121-125 SO_EXT:0000704 denotes gene
T57 158-167 GO:0010467 denotes expressed
T58 180-195 UBERON:0001016 denotes nervous systems
T59 213-219 PR_EXT:000003718 denotes ADAM22
T60 222-230 SO_EXT:biological_sequence denotes sequence
T61 278-286 GO_PR_EXT:integrin denotes integrin
T62 287-293 CHEMINF_GO_EXT:bearer_of_chemical_binding_or_bond_formation denotes binder
T63 321-325 CL_GO_EXT:cell denotes cell
T64 326-330 CL_GO_EXT:cell denotes cell
T65 334-338 CL_GO_EXT:cell denotes cell
T66 339-345 GO:0031012 denotes matrix
T67 377-390 GO_PATO_EXT:physiological_process_or_quality denotes physiological
T68 404-410 PR_EXT:000003718 denotes ADAM22
T69 424-428 SO_EXT:0000704 denotes gene
T70 451-457 PR_EXT:000003718 denotes ADAM22
T71 467-471 NCBITaxon:10088 denotes mice
T72 482-488 PR_EXT:000003718 denotes ADAM22
T73 499-503 NCBITaxon:10088 denotes mice
T74 587-592 GO:0007567 denotes birth
T75 658-662 GO:0016265 denotes died
T76 765-780 UBERON:0000956 denotes cerebral cortex
T77 784-794 UBERON:0002037 denotes cerebellum
T78 813-820 SO_EXT:sequence_altered_entity denotes mutants
T79 842-853 GO_EXT:myelin_assembly_or_myelination denotes myelination
T80 861-878 UBERON:0001021 denotes peripheral nerves
T81 947-953 PR_EXT:000003718 denotes ADAM22
T82 1008-1022 UBERON:0001016 denotes nervous system
T83 1044-1050 PR_EXT:000003718 denotes ADAM22
T84 1105-1110 NCBITaxon:9606 denotes human
T465 1210-1225 GO_EXT:0008237 denotes Metalloprotease
T466 1242-1250 GO:0016020 denotes membrane
T467 1266-1272 SO_EXT:0000417 denotes domain
T468 1273-1281 CHEBI_PR_EXT:protein denotes proteins
T469 1295-1312 GO_EXT:0008237 denotes metalloproteinase
T470 1318-1324 SO_EXT:0000417 denotes domain
T471 1348-1354 SO_EXT:0000417 denotes domain
T472 1410-1417 NCBITaxon:40674 denotes mammals
T473 1425-1435 CHEBI_GO_EXT:biological_process_or_quality_or_role denotes biological
T474 1466-1479 GO:0009566 denotes fertilization
T475 1481-1491 GO_EXT:muscle_structure_or_tissue_development denotes myogenesis
T476 1496-1508 GO:0022008 denotes neurogenesis
T477 1512-1523 GO:0006508 denotes proteolysis
T478 1528-1536 GO_PATO_EXT:biological_adhesion_entity_or_process denotes adhesion
T479 1561-1574 GO_MOP_EXT:catalysis denotes catalytically
T480 1582-1598 GO_EXT:0008237 denotes metalloproteases
T481 1612-1625 GO:0005576 denotes extracellular
T482 1617-1625 CL_GO_EXT:cell denotes cellular
T483 1626-1633 SO_EXT:0000417 denotes domains
T484 1637-1645 GO:0016020 denotes membrane
T485 1646-1651 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes bound
T486 1652-1658 GO_EXT:biological_growth_entity_or_process denotes growth
T487 1652-1666 GO_EXT:0008083 denotes growth factors
T488 1670-1679 GO_EXT:0004872 denotes receptors
T489 1700-1706 PR_EXT:000001279 denotes ADAM17
T490 1708-1712 PR_EXT:000001279 denotes TACE
T491 1732-1738 MOP:0000780 denotes cleave
T492 1769-1797 PR_EXT:000000134 denotes tumour necrosis factor alpha
T493 1776-1784 GO_PATO_EXT:necrosis denotes necrosis
T494 1804-1811 CHEBI:28304 denotes heparin
T495 1804-1862 PR_EXT:000008459 denotes heparin-binding epidermal growth factor-like growth factor
T496 1812-1819 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes binding
T497 1820-1829 UBERON:0007376 denotes epidermal
T498 1830-1836 GO_EXT:biological_growth_entity_or_process denotes growth
T499 1830-1843 GO_EXT:0008083 denotes growth factor
T500 1849-1855 GO_EXT:biological_growth_entity_or_process denotes growth
T501 1849-1862 GO_EXT:0008083 denotes growth factor
T502 1873-1905 PR_EXT:000016285 denotes transforming growth factor alpha
T503 1886-1892 GO_EXT:biological_growth_entity_or_process denotes growth
T504 1886-1899 GO_EXT:0008083 denotes growth factor
T505 1922-1928 PR_EXT:000001279 denotes ADAM17
T506 1929-1933 SO_EXT:sequence_nullness denotes null
T507 1934-1938 NCBITaxon:10088 denotes mice
T508 1958-1964 PR_EXT:000001279 denotes ADAM17
T509 1980-1993 GO:0009790 denotes embryogenesis
T510 2039-2045 GO_EXT:biological_growth_entity_or_process denotes growth
T511 2039-2053 GO_EXT:0008083 denotes growth factors
T512 2088-2092 CL_GO_EXT:cell denotes cell
T513 2093-2097 CL_GO_EXT:cell denotes cell
T514 2101-2105 CL_GO_EXT:cell denotes cell
T515 2106-2112 GO:0031012 denotes matrix
T516 2113-2121 GO_PATO_EXT:biological_adhesion_entity_or_process denotes adhesion
T517 2153-2162 GO_PR_EXT:integrin denotes integrins
T518 2166-2175 PR_EXT:syndecan denotes syndecans
T519 2223-2231 GO_PR_EXT:integrin denotes integrin
T520 2241-2245 CL_GO_EXT:cell denotes cell
T521 2246-2254 GO_PATO_EXT:biological_adhesion_entity_or_process denotes adhesion
T522 2295-2300 PR_EXT:000003715 denotes ADAM2
T523 2301-2305 SO_EXT:sequence_nullness denotes null
T524 2310-2315 PR_EXT:000003732 denotes ADAM3
T525 2316-2320 SO_EXT:sequence_nullness denotes null
T526 2321-2325 PATO_UBERON_EXT:male_or_bearer_of_maleness denotes male
T527 2326-2333 SO_EXT:sequence_altered_entity denotes mutants
T528 2358-2369 CL:0000019 denotes spermatozoa
T529 2375-2378 CL:0000025 denotes egg
T530 2379-2386 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes binding
T531 2411-2416 PR_EXT:000003715 denotes ADAM2
T532 2421-2426 PR_EXT:000003732 denotes ADAM3
T533 2435-2451 GO_EXT:0008237 denotes metalloproteases
T534 2470-2479 GO_MOP_EXT:catalysis denotes catalytic
T535 2485-2494 SO_EXT:biological_sequence denotes sequences
T536 2504-2519 GO_EXT:0008237 denotes metalloprotease
T537 2520-2526 SO_EXT:0000417 denotes domain
T538 2566-2576 GO_EXT:0008233 denotes proteinase
T539 2664-2670 PR_EXT:000003710 denotes ADAM11
T540 2672-2678 PR_EXT:000003718 denotes ADAM22
T541 2683-2689 PR_EXT:000003719 denotes ADAM23
T542 2690-2695 SO_EXT:0000704 denotes genes
T543 2717-2727 GO:0010467 denotes expression
T544 2735-2740 NCBITaxon:9606 denotes human
T545 2745-2751 NCBITaxon:39107 denotes murine
T546 2752-2767 UBERON:0001016 denotes nervous systems
T547 2777-2785 SO_EXT:biological_sequence denotes Sequence
T548 2822-2838 GO_EXT:0008237 denotes metalloproteases
T549 2862-2871 GO_MOP_EXT:catalysis denotes catalytic
T550 2872-2877 SO_EXT:sequence_or_structure_motif denotes motif
T551 2905-2911 PR_EXT:000003719 denotes ADAM23
T552 2912-2919 CHEBI_PR_EXT:protein denotes protein
T553 2923-2932 GO_PATO_EXT:biological_localization_process_or_quality denotes localised
T554 2940-2944 CL_GO_EXT:cell denotes cell
T555 2940-2952 GO:0009986 denotes cell surface
T556 2974-2997 GO:0034683 denotes alpha-v beta-3 integrin
T557 2982-2988 PR_EXT:000002042 denotes beta-3
T558 2989-2997 GO_PR_EXT:integrin denotes integrin
T559 3004-3009 GO_EXT:dimer_macromolecular_complex denotes dimer
T560 3037-3043 PR_EXT:000003719 denotes ADAM23
T561 3044-3048 SO_EXT:0000704 denotes gene
T562 3056-3061 NCBITaxon:10088 denotes mouse
T563 3083-3088 GO:0016265 denotes death
T564 3151-3156 GO:0016265 denotes death
T565 3165-3170 NCBITaxon:10088 denotes mouse
T566 3214-3218 CL_GO_EXT:cell denotes cell
T567 3219-3223 CL_GO_EXT:cell denotes cell
T568 3227-3231 CL_GO_EXT:cell denotes cell
T569 3232-3238 GO:0031012 denotes matrix
T570 3259-3273 UBERON:0001016 denotes nervous system
T571 3292-3298 PR_EXT:000003719 denotes ADAM23
T572 3319-3325 PR_EXT:000003718 denotes ADAM22
T573 3330-3336 PR_EXT:000003719 denotes ADAM23
T574 3350-3360 SO:0000857 denotes homologous
T575 3361-3370 SO_EXT:biological_sequence denotes sequences
T576 3380-3393 GO:0005576 denotes extracellular
T577 3385-3393 CL_GO_EXT:cell denotes cellular
T578 3394-3401 SO_EXT:0000417 denotes domains
T579 3447-3455 GO_PR_EXT:integrin denotes integrin
T580 3456-3463 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes binding
T581 3469-3478 SO_EXT:biological_sequence denotes sequences
T582 3542-3548 SO_EXT:0000417 denotes domain
T583 3592-3598 PR_EXT:000003718 denotes ADAM22
T584 3605-3613 GO_PR_EXT:integrin denotes integrin
T585 3614-3620 CHEMINF_GO_EXT:bearer_of_chemical_binding_or_bond_formation denotes binder
T586 3656-3670 UBERON:0001016 denotes nervous system
T587 3680-3686 PR_EXT:000003719 denotes ADAM23
T588 3705-3718 GO_PATO_EXT:physiological_process_or_quality denotes physiological
T589 3732-3738 PR_EXT:000003718 denotes ADAM22
T590 3766-3772 PR_EXT:000003718 denotes Adam22
T591 3773-3777 SO_EXT:0000704 denotes gene
T592 3787-3791 NCBITaxon:10088 denotes mice
T1175 3817-3823 PR_EXT:000003718 denotes ADAM22
T1176 3834-3838 NCBITaxon:10088 denotes mice
T1177 3839-3843 NCBITaxon:10088 denotes Mice
T1178 3864-3872 SO_EXT:sequence_alteration_entity_or_process denotes mutation
T1179 3882-3888 PR_EXT:000003718 denotes Adam22
T1180 3889-3893 SO_EXT:0000704 denotes gene
T1181 3923-3936 GO_SO_EXT:sequence_rearrangement_process denotes recombination
T1182 4035-4052 SO_EXT:0000319 denotes termination codon
T1183 4071-4075 SO_EXT:0000147 denotes exon
T1184 4085-4096 SO_EXT:0001062 denotes pro-protein
T1185 4089-4096 CHEBI_PR_EXT:protein denotes protein
T1186 4097-4103 SO_EXT:0000417 denotes domain
T1187 4114-4123 SO_EXT:sequence_truncation_process denotes truncated
T1188 4136-4142 PR_EXT:000003718 denotes ADAM22
T1189 4143-4150 CHEBI_PR_EXT:protein denotes protein
T1190 4191-4197 SO_EXT:0001023 denotes allele
T1191 4209-4220 SO_EXT:0001062 denotes pro-protein
T1192 4213-4220 CHEBI_PR_EXT:protein denotes protein
T1193 4221-4227 SO_EXT:0000417 denotes domain
T1194 4276-4284 CHEBI_PR_EXT:protein denotes proteins
T1195 4292-4297 PR_EXT:000003137 denotes Furin
T1196 4303-4312 GO_EXT:0008233 denotes proteases
T1197 4381-4390 SO_EXT:sequence_truncation_process denotes truncated
T1198 4399-4405 PR_EXT:000003718 denotes ADAM22
T1199 4406-4413 CHEBI_PR_EXT:protein denotes protein
T1200 4449-4455 PR_EXT:000003718 denotes ADAM22
T1201 4456-4463 CHEBI_PR_EXT:protein denotes protein
T1202 4478-4485 SO_EXT:sequence_altered_entity denotes mutants
T1203 4544-4552 GO:0042571 denotes antibody
T1204 4575-4586 GO:0005737 denotes cytoplasmic
T1205 4587-4596 _FRAGMENT denotes domain of
T1206 4608-4615 SO_EXT:0000417 denotes protein
T1207 4601-4607 PR_EXT:000003718 denotes ADAM22
T1208 4608-4615 CHEBI_PR_EXT:protein denotes protein
T1209 4638-4645 SO_EXT:sequence_altered_entity denotes mutants
T1210 4678-4683 GO:0007567 denotes birth
T1211 4716-4725 SO_EXT:wild_type_entity_or_quality denotes wild-type
T1212 4784-4789 GO:0007567 denotes natal
T1213 4827-4834 SO_EXT:sequence_altered_entity denotes mutants
T1214 4889-4893 UBERON_EXT:body denotes body
T1215 4919-4928 GO_EXT:biological_movement_or_translocation_process denotes movements
T1216 4938-4943 UBERON:0002101 denotes limbs
T1217 5018-5021 GO:0016265 denotes die
T1218 5056-5060 UBERON_EXT:body denotes body
T1219 5076-5084 SO_EXT:genotype_or_entity_with_genotype denotes genotype
T1220 5124-5128 PATO_UBERON_EXT:male_or_bearer_of_maleness denotes male
T1221 5129-5136 SO_EXT:sequence_altered_entity denotes mutants
T1222 5148-5155 PATO_UBERON_EXT:female_or_bearer_of_femaleness denotes females
T1223 5208-5213 PATO_UBERON_EXT:male_or_bearer_of_maleness denotes males
T1224 5218-5225 PATO_UBERON_EXT:female_or_bearer_of_femaleness denotes females
T1225 5319-5327 SO_EXT:genotype_or_entity_with_genotype denotes genotype
T1226 5389-5397 SO_EXT:genotype_or_entity_with_genotype denotes genotype
T1227 5454-5455 SO_EXT:normal_or_wild_type_or_present denotes +
T1228 5456-5457 SO_EXT:normal_or_wild_type_or_present denotes +
T1229 5465-5466 SO_EXT:normal_or_wild_type_or_present denotes +
T1230 5467-5468 SO_EXT:sequence_nullness_or_absence denotes -
T1231 5476-5477 SO_EXT:sequence_nullness_or_absence denotes -
T1232 5478-5479 SO_EXT:sequence_nullness_or_absence denotes -
T1233 5514-5520 PR_EXT:000003718 denotes ADAM22
T1234 5542-5555 GO:0009790 denotes embryogenesis
T1235 5577-5581 UBERON_EXT:body denotes body
T1236 5603-5610 SO_EXT:sequence_altered_entity denotes mutants
T1237 5642-5651 SO_EXT:wild_type_entity_or_quality denotes wild-type
T1238 5703-5710 SO_EXT:sequence_altered_entity denotes mutants
T1239 5711-5715 GO:0016265 denotes died
T1240 5758-5762 GO:0016265 denotes died
T1241 5865-5870 GO:0007567 denotes birth
T1242 5896-5903 SO_EXT:sequence_altered_entity denotes mutants
T1839 6013-6019 PR_EXT:000003718 denotes ADAM22
T1840 6030-6034 NCBITaxon:10088 denotes mice
T1841 6068-6078 GO:0010467 denotes expression
T1842 6082-6088 PR_EXT:000003718 denotes ADAM22
T1843 6089-6093 CHEBI_SO_EXT:mRNA denotes mRNA
T1844 6101-6106 NCBITaxon:9606 denotes human
T1845 6111-6116 NCBITaxon:10088 denotes mouse
T1846 6117-6122 UBERON:0000955 denotes brain
T1847 6191-6197 PR_EXT:000003718 denotes ADAM22
T1848 6198-6208 SO_EXT:0000673 denotes transcript
T1849 6216-6221 UBERON:0007023 denotes adult
T1850 6222-6227 NCBITaxon:10088 denotes mouse
T1851 6228-6231 UBERON:0001017 denotes CNS
T1852 6251-6264 GO:0097617 denotes hybridisation
T1853 6294-6297 CHEBI:37983 denotes 35S
T1854 6298-6305 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes labeled
T1855 6306-6315 CHEBI_SO_EXT:riboprobe denotes RNA probe
T1856 6337-6343 PR_EXT:000003718 denotes ADAM22
T1857 6344-6348 CHEBI_SO_EXT:mRNA denotes mRNA
T1858 6353-6362 GO:0010467 denotes expressed
T1859 6378-6383 UBERON:0007023 denotes adult
T1860 6384-6389 NCBITaxon:10088 denotes mouse
T1861 6390-6393 UBERON:0001017 denotes CNS
T1862 6429-6439 UBERON:0002037 denotes cerebellar
T1863 6429-6453 CL:0001031 denotes cerebellar granule cells
T1864 6448-6453 CL_GO_EXT:cell denotes cells
T1865 6458-6479 UBERON:0002421 denotes hippocampal formation
T1866 6488-6499 UBERON:0002240 denotes spinal cord
T1867 6501-6514 GO:0097617 denotes hybridisation
T1868 6544-6555 UBERON:0002020 denotes grey matter
T1869 6577-6583 PR_EXT:000003718 denotes ADAM22
T1870 6584-6595 SO_EXT:0000673 denotes transcripts
T1871 6603-6606 UBERON:0001017 denotes CNS
T1872 6638-6644 PR_EXT:000003710 denotes ADAM11
T1873 6652-6660 CL:0000540 denotes neuronal
T1874 6661-6671 GO:0010467 denotes expression
T1875 6727-6733 PR_EXT:000003718 denotes ADAM22
T1876 6734-6738 CHEBI_SO_EXT:mRNA denotes mRNA
T1877 6739-6749 GO:0010467 denotes expression
T1878 6753-6761 CL:0000540 denotes neuronal
T1879 6769-6774 NCBITaxon:10088 denotes mouse
T1880 6775-6778 UBERON:0001017 denotes CNS
T1881 6793-6797 CHEBI_SO_EXT:mRNA denotes mRNA
T1882 6873-6883 UBERON:0002037 denotes cerebellum
T1883 6888-6899 UBERON_EXT:hippocampus_proper_or_hippocampal_formation denotes hippocampus
T1884 6939-6949 GO:0010467 denotes expression
T1885 6953-6959 PR_EXT:000003718 denotes ADAM22
T1886 6960-6965 CHEBI_SO_EXT:mRNA denotes mRNAs
T1887 6973-6983 UBERON:0002037 denotes cerebellar
T1888 6973-6997 CL:0001031 denotes cerebellar granule cells
T1889 6992-6997 CL_GO_EXT:cell denotes cells
T1890 6999-7011 CL:0000120 denotes granule cell
T1891 7007-7011 CL_GO_EXT:cell denotes cell
T1892 7042-7055 CL:0000121 denotes Purkinje cell
T1893 7051-7055 CL_GO_EXT:cell denotes cell
T1894 7068-7077 PR_EXT:000004967 denotes calbindin
T1895 7095-7101 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T1896 7102-7107 NCBITaxon:10088 denotes mouse
T1897 7136-7157 UBERON:0002421 denotes Hippocampal formation
T1898 7224-7237 CL:0000540 denotes neuronal cell
T1899 7224-7247 GO:0001764 denotes neuronal cell migration
T1900 7233-7237 CL_GO_EXT:cell denotes cell
T1901 7272-7278 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T1902 7279-7284 NCBITaxon:10088 denotes mouse
T1903 7286-7302 GO_EXT:myelin_assembly_or_myelination denotes Myelin-formation
T1904 7315-7318 PR_EXT:000010228 denotes MBP
T1905 7320-7326 UBERON:0000345 denotes myelin
T1906 7320-7340 PR_EXT:000010228 denotes myelin basic protein
T1907 7327-7332 CHEBI_EXT:22695 denotes basic
T1908 7333-7340 CHEBI_PR_EXT:protein denotes protein
T1909 7358-7364 SO_EXT:sequence_altered_entity denotes mutant
T1910 7372-7382 UBERON:0002037 denotes cerebellum
T1911 7397-7408 UBERON:0002240 denotes spinal cord
T1912 7455-7464 SO_EXT:wild_type_entity_or_quality denotes wild-type
T1913 7554-7560 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T1914 7561-7566 NCBITaxon:10088 denotes mouse
T1915 7567-7572 UBERON:0000955 denotes brain
T1916 7621-7632 UBERON:0002240 denotes spinal cord
T1917 7637-7654 UBERON:0001021 denotes peripheral nerves
T1918 7663-7671 SO_EXT:genotype_or_entity_with_genotype denotes genotype
T1919 7725-7731 UBERON:0000345 denotes myelin
T1920 7725-7741 GO_EXT:myelin_assembly_or_myelination denotes myelin formation
T1921 7765-7771 UBERON:0000345 denotes myelin
T1922 7780-7786 UBERON:0000345 denotes myelin
T1923 7807-7821 UBERON:0001322 denotes sciatic nerves
T1924 7836-7853 UBERON:0001645 denotes trigeminal nerves
T1925 7878-7885 SO_EXT:sequence_altered_entity denotes mutants
T1926 7927-7938 UBERON:0002240 denotes spinal cord
T1927 7972-7984 CL:0002573 denotes Schwann cell
T1928 7980-7984 CL_GO_EXT:cell denotes cell
T1929 7994-8005 GO_EXT:myelin_assembly_or_myelination denotes myelination
T1930 8040-8044 NCBITaxon:10088 denotes mice
T1931 8070-8081 GO_EXT:myelin_assembly_or_myelination denotes myelinating
T1932 8070-8095 CL:0000218 denotes myelinating Schwann cells
T1933 8090-8095 CL_GO_EXT:cell denotes cells
T1934 8100-8105 GO:0030424 denotes axons
T1935 8107-8115 CHEBI:10545 denotes electron
T1936 8149-8162 UBERON:0001322 denotes sciatic nerve
T1937 8187-8200 CL:0002573 denotes Schwann cells
T1938 8195-8200 CL_GO_EXT:cell denotes cells
T1939 8219-8225 UBERON:0000345 denotes myelin
T1940 8244-8250 SO_EXT:sequence_altered_entity denotes mutant
T1941 8288-8292 NCBITaxon:10088 denotes mice
T1942 8309-8320 GO_EXT:myelin_assembly_or_myelination denotes myelination
T1943 8350-8355 GO:0030424 denotes axons
T1944 8378-8386 SO_EXT:genotype_or_entity_with_genotype denotes genotype
T1945 8424-8437 UBERON:0001322 denotes sciatic nerve
T1946 8470-8476 GO:0005634 denotes nuclei
T1947 8511-8514 PR_EXT:000010228 denotes MBP
T1948 8559-8565 SO_EXT:sequence_altered_entity denotes mutant
T1949 8571-8583 CL:0002573 denotes Schwann cell
T1950 8579-8583 CL_GO_EXT:cell denotes cell
T1951 8584-8590 CHEBI_SO_EXT:molecular_indicator_or_label_or_marker_or_tag denotes marker
T1952 8665-8674 SO_EXT:wild_type_entity_or_quality denotes wild-type
T1953 8717-8733 _FRAGMENT denotes proliferation of
T1954 8738-8751 GO:0014010 denotes Schwann cells
T1955 8738-8751 CL:0002573 denotes Schwann cells
T1956 8746-8751 CL_GO_EXT:cell denotes cells
T1957 8773-8788 GO_RO_EXT:developmental_differentiation_process denotes differentiation
T1958 8816-8822 PR_EXT:000003718 denotes ADAM22
T1959 8833-8837 NCBITaxon:10088 denotes mice
T2635 8846-8852 PR_EXT:000003718 denotes ADAM22
T2636 8853-8863 SO_EXT:0000673 denotes transcript
T2637 8864-8872 SO_EXT:sequence_variant_entity denotes variants
T2638 8891-8916 UBERON:0000010 denotes peripheral nervous system
T2639 8932-8937 NCBITaxon:9606 denotes human
T2640 8938-8944 PR_EXT:000003718 denotes ADAM22
T2641 8964-8972 GO:0008380 denotes splicing
T2642 8964-8981 SO_EXT:alternative_splice_variant denotes splicing variants
T2643 9015-9020 SO_EXT:0000147 denotes exons
T2644 9084-9088 SO_EXT:0000147 denotes exon
T2645 9090-9094 SO_EXT:0000147 denotes exon
T2646 9106-9111 NCBITaxon:10088 denotes mouse
T2647 9177-9182 NCBITaxon:10088 denotes mouse
T2648 9183-9189 PR_EXT:000003718 denotes ADAM22
T2649 9190-9201 SO_EXT:0000673 denotes transcripts
T2650 9227-9232 NCBITaxon:10088 denotes mouse
T2651 9233-9239 SO_EXT:0001026 denotes genome
T2652 9249-9257 SO:0000857 denotes homology
T2653 9297-9302 NCBITaxon:10088 denotes mouse
T2654 9303-9309 SO_EXT:0001026 denotes genome
T2655 9310-9316 SO_EXT:0000149 denotes contig
T2656 9349-9354 NCBITaxon:10088 denotes mouse
T2657 9355-9361 PR_EXT:000003718 denotes Adam22
T2658 9362-9366 SO_EXT:0000704 denotes gene
T2659 9376-9382 SO_EXT:0000149 denotes contig
T2660 9383-9391 SO_EXT:biological_sequence denotes sequence
T2661 9411-9416 SO_EXT:0000147 denotes exons
T2662 9434-9443 SO_EXT:biological_sequence denotes sequences
T2663 9466-9471 NCBITaxon:9606 denotes human
T2664 9472-9478 PR_EXT:000003718 denotes ADAM22
T2665 9479-9486 SO_EXT:0001060 denotes isoform
T2666 9536-9547 SO_EXT:0000673 denotes transcripts
T2667 9561-9568 SO_EXT:0000112 denotes primers
T2668 9585-9589 SO_EXT:0000147 denotes exon
T2669 9607-9609 GO_EXT:reverse_transcription_or_reverse_transcriptase denotes RT
T2670 9620-9625 NCBITaxon:10088 denotes mouse
T2671 9626-9636 UBERON:0002037 denotes cerebellum
T2672 9637-9641 CHEBI_SO_EXT:mRNA denotes mRNA
T2673 9660-9667 SO_EXT:sequence_cloning_process denotes cloning
T2674 9738-9744 SO_EXT:sequence_cloned_entity denotes clones
T2675 9757-9764 SO_EXT:0001060 denotes isoform
T2676 9774-9780 SO_EXT:sequence_cloned_entity denotes clones
T2677 9793-9800 SO_EXT:0001060 denotes isoform
T2678 9813-9818 SO_EXT:sequence_cloned_entity denotes clone
T2679 9843-9847 SO_EXT:0000147 denotes exon
T2680 9856-9861 SO_EXT:0000147 denotes exons
T2681 9892-9894 GO_EXT:reverse_transcription_or_reverse_transcriptase denotes RT
T2682 9911-9916 CHEBI_SO_EXT:mRNA denotes mRNAs
T2683 9939-9944 UBERON:0007023 denotes adult
T2684 9945-9950 NCBITaxon:10088 denotes mouse
T2685 9951-9958 UBERON:0000479 denotes tissues
T2686 9972-9983 UBERON:0002240 denotes spinal cord
T2687 9985-10005 UBERON:0000044 denotes dorsal root ganglion
T2688 10007-10010 UBERON:0000044 denotes DRG
T2689 10013-10026 UBERON:0001322 denotes sciatic nerve
T2690 10031-10047 _FRAGMENT denotes cultured primary
T2691 10056-10061 CL:0000001 denotes cells
T2692 10048-10061 CL:0002573 denotes Schwann cells
T2693 10056-10061 CL_GO_EXT:cell denotes cells
T2694 10087-10090 CHEBI_SO_EXT:DNA denotes DNA
T2695 10126-10132 UBERON:0000479 denotes tissue
T2696 10263-10276 UBERON:0001322 denotes sciatic nerve
T2697 10299-10307 _FRAGMENT denotes cultured
T2698 10316-10321 CL:0000010 denotes cells
T2699 10308-10321 CL:0002573 denotes Schwann cells
T2700 10316-10321 CL_GO_EXT:cell denotes cells
T2701 10393-10400 UBERON:0000479 denotes tissues
T2702 10440-10443 UBERON:0000044 denotes DRG
T2703 10494-10501 SO_EXT:sequence_cloning_process denotes cloning
T2704 10528-10532 SO_EXT:0000147 denotes exon
T2705 10563-10574 SO_EXT:0000673 denotes transcripts
T2706 10598-10604 SO_EXT:sequence_cloned_entity denotes clones
T2707 10666-10672 SO_EXT:sequence_cloned_entity denotes clones
T2708 10706-10713 SO_EXT:0001026 denotes genomic
T2709 10714-10722 SO_EXT:biological_sequence denotes sequence
T2710 10757-10762 SO_EXT:0000147 denotes exons
T2711 10764-10769 SO_EXT:0000147 denotes exons
T2712 10828-10833 SO_EXT:0000147 denotes exons
T2713 10839-10846 SO:0000357 denotes flanked
T2714 10863-10870 SO_EXT:0000188 denotes introns
T2715 10897-10901 SO_EXT:0000147 denotes Exon
T2716 10923-10928 SO_EXT:sequence_cloned_entity denotes clone
T2717 10977-10983 PR_EXT:000003718 denotes ADAM22
T2718 10984-10995 SO_EXT:0000673 denotes transcripts
T2719 11015-11024 SO_EXT:sequence_insertion_entity_or_process denotes insertion
T2720 11040-11045 SO_EXT:0000147 denotes exons
T2721 11069-11080 CHEBI_SO_EXT:nucleotide denotes nucleotides
T2722 11084-11089 SO_EXT:0000147 denotes exons
T2723 11143-11152 SO_EXT:sequence_insertion_entity_or_process denotes insertion
T2724 11174-11179 SO_EXT:0000147 denotes exons
T2725 11189-11195 SO_EXT:sequence_alteration_process denotes change
T2726 11200-11210 SO_EXT:sequence_downstreamness denotes downstream
T2727 11211-11224 SO_EXT:0000717 denotes reading frame
T2728 11232-11239 CHEBI_SO_EXT:peptide_or_peptide_region denotes peptide
T2729 11240-11247 SO_EXT:sequence_or_structure_motif denotes motives
T2730 11285-11294 SO_EXT:biological_sequence denotes sequences
T2731 11301-11303 GO_EXT:reverse_transcription_or_reverse_transcriptase denotes RT
T2732 11343-11349 PR_EXT:000003718 denotes ADAM22
T2733 11350-11361 SO_EXT:0000673 denotes transcripts
T2734 11399-11402 UBERON:0001017 denotes CNS
T2735 11420-11424 SO_EXT:0000147 denotes exon
T2736 11430-11433 UBERON:0000044 denotes DRG
T2737 11451-11455 SO_EXT:0000147 denotes exon
T2738 11465-11477 CL:0002573 denotes Schwann-cell
T2739 11473-11477 CL_GO_EXT:cell denotes cell
T2740 11493-11497 SO_EXT:0000147 denotes exon
T2741 11548-11554 PR_EXT:000003718 denotes ADAM22
T2742 11558-11567 GO:0010467 denotes expressed
T2743 11573-11577 CL_GO_EXT:cell denotes cell
T2744 11631-11645 GO_EXT:myelin_assembly_or_myelination denotes myelinogenesis
T2745 11653-11656 UBERON:0000010 denotes PNS
T3558 15064-15068 CL_GO_EXT:cell denotes cell
T3559 11720-11726 PR_EXT:000003718 denotes ADAM22
T3560 11741-11745 NCBITaxon:10088 denotes mice
T3561 11762-11768 PR_EXT:000003718 denotes Adam22
T3562 11769-11773 SO_EXT:0000704 denotes gene
T3563 11775-11781 PR_EXT:000003718 denotes ADAM22
T3564 11792-11796 NCBITaxon:10088 denotes mice
T3565 11835-11840 GO:0016265 denotes death
T3566 11868-11875 SO_EXT:sequence_altered_entity denotes mutants
T3567 11972-11977 GO:0016265 denotes death
T3568 11996-12003 SO_EXT:sequence_altered_entity denotes mutants
T3569 12176-12180 NCBITaxon:10088 denotes mice
T3570 12220-12224 GO:0016265 denotes died
T3571 12259-12267 UBERON:0001851 denotes cortical
T3572 12302-12307 UBERON:0000955 denotes brain
T3573 12302-12319 GO:0007420 denotes brain development
T3574 12363-12367 NCBITaxon:10088 denotes mice
T3575 12380-12386 CL:0000540 denotes neuron
T3576 12396-12417 PR_EXT:000005259 denotes activator of cyclin 5
T3577 12419-12422 PR_EXT:000005259 denotes p35
T3578 12454-12462 CL:0000540 denotes neuronal
T3579 12454-12472 GO:0001764 denotes neuronal migration
T3580 12494-12500 PR_EXT:000003718 denotes ADAM22
T3581 12501-12508 CHEBI_PR_EXT:protein denotes protein
T3582 12512-12521 GO:0010467 denotes expressed
T3583 12529-12533 CL_GO_EXT:cell denotes cell
T3584 12529-12541 GO:0009986 denotes cell surface
T3585 12574-12578 CL_GO_EXT:cell denotes cell
T3586 12579-12583 CL_GO_EXT:cell denotes cell
T3587 12587-12591 CL_GO_EXT:cell denotes cell
T3588 12592-12598 GO:0031012 denotes matrix
T3589 12647-12653 PR_EXT:000003718 denotes ADAM22
T3590 12678-12686 CL:0000540 denotes neuronal
T3591 12678-12696 GO:0001764 denotes neuronal migration
T3592 12816-12822 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T3593 12823-12828 NCBITaxon:10088 denotes mouse
T3594 12829-12834 UBERON:0000955 denotes brain
T3595 12867-12873 PR_EXT:000003718 denotes ADAM22
T3596 12877-12885 CL:0000540 denotes neuronal
T3597 12877-12895 GO:0001764 denotes neuronal migration
T3598 12948-12953 GO:0016265 denotes death
T3599 12974-12978 UBERON:0000104 denotes life
T3600 13015-13039 UBERON:0002410 denotes autonomic nervous system
T3601 13053-13059 UBERON:0001021 denotes nerves
T3602 13066-13073 GO:0002087 denotes control
T3603 13125-13143 UBERON:0001004 denotes respiration system
T3604 13166-13176 GO_PATO_EXT:biological_maturation denotes maturation
T3605 13206-13211 GO:0007567 denotes birth
T3606 13230-13236 UBERON:0000345 denotes myelin
T3607 13252-13255 NCBITaxon:10114 denotes rat
T3608 13273-13287 SO_EXT:point_mutation_entity_or_process denotes point mutation
T3609 13295-13306 CHEBI_GO_EXT:lipoprotein_particle denotes proteolipid
T3610 13295-13314 PR_EXT:000012879 denotes proteolipid protein
T3611 13307-13314 CHEBI_PR_EXT:protein denotes protein
T3612 13316-13319 PR_EXT:000012879 denotes PLP
T3613 13321-13325 SO_EXT:0000704 denotes gene
T3614 13355-13366 GO_EXT:myelin_assembly_or_myelination denotes myelination
T3615 13371-13375 GO:0016265 denotes dies
T3616 13397-13402 GO:0007567 denotes natal
T3617 13417-13420 NCBITaxon:10114 denotes rat
T3618 13428-13433 GO:0016265 denotes death
T3619 13470-13480 UBERON:0002298 denotes brain stem
T3620 13504-13536 GO:0002087 denotes autonomic control of respiration
T3621 13605-13610 GO:0016265 denotes death
T3622 13614-13620 PR_EXT:000003718 denotes ADAM22
T3623 13631-13635 NCBITaxon:10088 denotes mice
T3624 13679-13685 PR_EXT:000003718 denotes ADAM22
T3625 13696-13700 NCBITaxon:10088 denotes mice
T3626 13709-13723 GO_EXT:myelin_assembly_or_myelination denotes myelinogenesis
T3627 13772-13783 GO_EXT:myelin_assembly_or_myelination denotes myelination
T3628 13791-13798 _FRAGMENT denotes sciatic
T3629 13814-13820 UBERON:0001322 denotes nerves
T3630 13803-13820 UBERON:0001645 denotes trigeminal nerves
T3631 13831-13837 UBERON:0000345 denotes myelin
T3632 13831-13847 GO_EXT:myelin_assembly_or_myelination denotes myelin formation
T3633 13855-13866 UBERON:0002240 denotes spinal cord
T3634 13875-13880 UBERON:0000955 denotes brain
T3635 13927-13941 GO_EXT:myelin_assembly_or_myelination denotes myelinogenesis
T3636 13971-13988 UBERON:0001021 denotes peripheral nerves
T3637 14005-14009 NCBITaxon:10088 denotes mice
T3638 14022-14034 CL:0002573 denotes Schwann cell
T3639 14030-14034 CL_GO_EXT:cell denotes cell
T3640 14055-14061 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T3641 14062-14075 UBERON:0001322 denotes sciatic nerve
T3642 14092-14121 CL:0000218 denotes pro-myelinating Schwann cells
T3643 14096-14107 GO_EXT:myelin_assembly_or_myelination denotes myelinating
T3644 14116-14121 CL_GO_EXT:cell denotes cells
T3645 14138-14141 PR_EXT:000010228 denotes MBP
T3646 14212-14240 CL:0000218 denotes pro-myelinating Schwann cell
T3647 14216-14227 GO_EXT:myelin_assembly_or_myelination denotes myelinating
T3648 14236-14240 CL_GO_EXT:cell denotes cell
T3649 14269-14275 UBERON:0000345 denotes myelin
T3650 14269-14285 GO_EXT:myelin_assembly_or_myelination denotes myelin formation
T3651 14300-14306 PR_EXT:000003718 denotes ADAM22
T3652 14334-14346 CL:0002573 denotes Schwann cell
T3653 14334-14346 _FRAGMENT denotes Schwann cell
T3654 14375-14388 GO:0014010 denotes proliferation
T3655 14342-14346 CL_GO_EXT:cell denotes cell
T3656 14342-14362 GO:0030154 denotes cell differentiation
T3657 14416-14430 GO_EXT:myelin_assembly_or_myelination denotes myelinogenesis
T3658 14443-14449 PR_EXT:000003718 denotes ADAM22
T3659 14460-14464 NCBITaxon:10088 denotes mice
T3660 14475-14483 GO_PR_EXT:integrin denotes integrin
T3661 14548-14557 GO_PR_EXT:integrin denotes integrins
T3662 14574-14581 SO_EXT:0000417 denotes domains
T3663 14618-14625 PR_EXT:000009131 denotes alpha-6
T3664 14618-14641 GO:0034675 denotes alpha-6 beta-1 integrin
T3665 14626-14632 PR_EXT:000002040 denotes beta-1
T3666 14633-14641 GO_PR_EXT:integrin denotes integrin
T3667 14649-14661 CL:0002573 denotes Schwann cell
T3668 14657-14661 CL_GO_EXT:cell denotes cell
T3669 14689-14695 UBERON:0000345 denotes myelin
T3670 14689-14705 GO_EXT:myelin_assembly_or_myelination denotes myelin formation
T3671 14725-14734 GO:0005607 denotes laminin-2
T3672 14765-14771 UBERON:0000345 denotes myelin
T3673 14765-14781 GO_EXT:myelin_assembly_or_myelination denotes myelin formation
T3674 14791-14800 GO:0005607 denotes laminin-2
T3675 14822-14833 GO_EXT:myelin_assembly_or_myelination denotes myelination
T3676 14837-14841 NCBITaxon:10088 denotes mice
T3677 14846-14852 NCBITaxon:9606 denotes humans
T3678 14862-14871 GO_EXT:0004872 denotes receptors
T3679 14873-14880 PR_EXT:000009131 denotes alpha-6
T3680 14873-14896 GO:0034675 denotes alpha-6 beta-1 integrin
T3681 14881-14887 PR_EXT:000002040 denotes beta-1
T3682 14888-14896 GO_PR_EXT:integrin denotes integrin
T3683 14901-14913 PR_EXT:000006267 denotes dystroglycan
T3684 14955-14961 UBERON:0000345 denotes myelin
T3685 14955-14971 GO_EXT:myelin_assembly_or_myelination denotes myelin formation
T3686 15017-15032 PR_EXT:000002040 denotes beta-1 integrin
T3687 15024-15032 GO_PR_EXT:integrin denotes integrin
T3688 15037-15052 PR_EXT:000009658 denotes laminin gamma-1
T3689 15056-15068 CL:0002573 denotes Schwann cell
T3690 15089-15098 GO:0005607 denotes laminin-2
T3691 15103-15112 GO_PR_EXT:integrin denotes integrins
T3692 15163-15168 GO:0030424 denotes axons
T3693 15191-15197 UBERON:0000345 denotes myelin
T3694 15191-15207 GO_EXT:myelin_assembly_or_myelination denotes myelin formation
T3695 15256-15262 PR_EXT:000003718 denotes ADAM22
T3696 15273-15277 NCBITaxon:10088 denotes mice
T3697 15332-15337 GO:0030424 denotes axons
T3698 15355-15361 UBERON:0001021 denotes nerves
T3699 15366-15371 UBERON:0002211 denotes roots
T3700 15388-15394 PR_EXT:000003718 denotes ADAM22
T3701 15405-15414 GO:0065007 denotes modulates
T3702 15415-15423 GO_PR_EXT:integrin denotes integrin
T3703 15476-15482 UBERON:0000345 denotes myelin
T3704 15476-15492 GO_EXT:myelin_assembly_or_myelination denotes myelin formation
T4452 15531-15537 PR_EXT:000003718 denotes ADAM22
T4453 15548-15552 NCBITaxon:10088 denotes mice
T4454 15569-15575 PR_EXT:000003718 denotes ADAM22
T4455 15612-15615 UBERON:0001017 denotes CNS
T4456 15624-15627 UBERON:0000010 denotes PNS
T4457 15668-15674 PR_EXT:000003718 denotes ADAM22
T4458 15690-15695 NCBITaxon:9606 denotes human
T4459 15753-15758 NCBITaxon:9606 denotes human
T4460 15759-15765 PR_EXT:000003718 denotes ADAM22
T4461 15766-15770 SO_EXT:0000704 denotes gene
T4462 15800-15801 CHEBI_SO_EXT:base denotes b
T4463 15846-15851 SO_EXT:0000147 denotes exons
T4464 15891-15902 GO_SO_EXT:chromosome denotes chromosomal
T4465 15939-15951 UBERON:0001016 denotes neurological
T4466 16009-16018 CHEBI_EXT:polyatomic_entity_or_group denotes molecular
T4467 16040-16046 PR_EXT:000003718 denotes ADAM22
T4468 16119-16124 NCBITaxon:9606 denotes human
T4469 16125-16137 UBERON:0001016 denotes neurological
T4618 16177-16183 PR_EXT:000003718 denotes Adam22
T4619 16184-16188 SO_EXT:0000704 denotes gene
T4620 16189-16205 SO_EXT:0001644 denotes targeting vector
T4621 16214-16215 CHEBI_SO_EXT:base denotes b
T4622 16216-16227 SO_EXT:genomic_DNA denotes genomic DNA
T4623 16224-16227 CHEBI_SO_EXT:DNA denotes DNA
T4624 16247-16252 SO_EXT:0000147 denotes exons
T4625 16277-16283 PR_EXT:000003718 denotes Adam22
T4626 16284-16288 SO_EXT:0000704 denotes gene
T4627 16317-16328 SO_EXT:genomic_DNA denotes genomic DNA
T4628 16325-16328 CHEBI_SO_EXT:DNA denotes DNA
T4629 16361-16367 CHEBI_GO_SO_EXT:enzyme denotes enzyme
T4630 16368-16371 CHEBI:60004 denotes mix
T4631 16396-16403 SO_EXT:0000112 denotes primers
T4632 16422-16426 SO_EXT:0000147 denotes exon
T4633 16442-16450 SO_EXT:sequence_alteration_entity_or_process denotes mutation
T4634 16458-16463 NCBITaxon:10088 denotes mouse
T4635 16464-16470 PR_EXT:000003718 denotes Adam22
T4636 16471-16475 SO_EXT:0000704 denotes gene
T4637 16480-16488 SO_EXT:sequence_insertion_process denotes inserted
T4638 16500-16508 SO_EXT:0005853 denotes cassette
T4639 16514-16518 SO_EXT:0000147 denotes exon
T4640 16528-16534 PR_EXT:000003718 denotes Adam22
T4641 16535-16539 SO_EXT:0000704 denotes gene
T4642 16561-16570 SO_EXT:engineered_biological_sequence denotes construct
T4643 16592-16593 CHEBI_SO_EXT:base denotes b
T4644 16594-16605 SO_EXT:genomic_DNA denotes genomic DNA
T4645 16602-16605 CHEBI_SO_EXT:DNA denotes DNA
T4646 16643-16652 SO_EXT:sequence_insertion_entity_or_process denotes insertion
T4647 16667-16675 SO_EXT:0005853 denotes cassette
T4648 16696-16698 GO_PR_EXT:thymidine_kinase denotes TK
T4649 16699-16707 SO_EXT:0005853 denotes cassette
T4650 16732-16738 CHEBI_SO_EXT:molecular_indicator_or_label_or_marker_or_tag denotes marker
T4815 16786-16792 PR_EXT:000003718 denotes Adam22
T4816 16803-16807 NCBITaxon:10088 denotes mice
T4817 16812-16822 SO_EXT:sequence_linearization denotes linearised
T4818 16823-16839 SO_EXT:0001644 denotes targeting vector
T4819 16868-16877 UBERON:0000922 denotes embryonic
T4820 16868-16882 _FRAGMENT denotes embryonic stem
T4821 16888-16893 CL:0002322 denotes cells
T4822 16884-16886 _FRAGMENT denotes ES
T4823 16888-16893 CL_GO_EXT:cell denotes cells
T4824 16911-16923 SO_EXT:sequence_recombinant_entity denotes recombinants
T4825 16960-16971 SO_EXT:genomic_DNA denotes genomic DNA
T4826 16968-16971 CHEBI_SO_EXT:DNA denotes DNA
T4827 16982-16987 SO_EXT:sequence_cloned_entity denotes clone
T4828 17012-17026 SO_EXT:0000121 denotes forward primer
T4829 17084-17092 SO_EXT:0005853 denotes cassette
T4830 17102-17116 SO_EXT:0000132 denotes reverse primer
T4831 17173-17189 SO_EXT:0001644 denotes targeting vector
T4832 17202-17212 GO_SO_EXT:sequence_rearrangement_process denotes recombined
T4833 17213-17216 CHEBI_SO_EXT:DNA denotes DNA
T4834 17354-17363 SO_EXT:engineered_biological_sequence denotes construct
T4835 17397-17399 CL:0002322 denotes ES
T4836 17400-17406 SO_EXT:sequence_cloned_entity denotes clones
T4837 17426-17436 GO:0009566 denotes fertilized
T4838 17441-17446 NCBITaxon:10088 denotes mouse
T4839 17447-17451 CL:0000025 denotes eggs
T4840 17459-17475 UBERON:0007236 denotes eight-cell stage
T4841 17465-17469 CL_GO_EXT:cell denotes cell
T4842 17491-17495 PATO_UBERON_EXT:male_or_bearer_of_maleness denotes male
T4843 17510-17515 GO:0007618 denotes mated
T4844 17530-17537 PATO_UBERON_EXT:female_or_bearer_of_femaleness denotes females
T4845 17556-17560 PATO_UBERON_EXT:male_or_bearer_of_maleness denotes male
T4846 17565-17571 PATO_UBERON_EXT:female_or_bearer_of_femaleness denotes female
T4847 17572-17576 NCBITaxon:10088 denotes mice
T4848 17587-17591 GO_EXT:breeding denotes bred
T4849 17615-17619 NCBITaxon:10088 denotes mice
T4850 17657-17661 NCBITaxon:10088 denotes mice
T5041 17726-17731 NCBITaxon:10088 denotes Mouse
T5042 17732-17743 SO_EXT:genomic_DNA denotes genomic DNA
T5043 17740-17743 CHEBI_SO_EXT:DNA denotes DNA
T5044 17799-17804 UBERON:0002107 denotes liver
T5045 17813-17821 SO_EXT:genotype_or_entity_with_genotype denotes genotype
T5046 17823-17828 PR_EXT:P23940 denotes BamHI
T5047 17838-17849 SO_EXT:genomic_DNA denotes genomic DNA
T5048 17846-17849 CHEBI_SO_EXT:DNA denotes DNA
T5049 17871-17874 CHEBI:37972 denotes 32P
T5050 17876-17884 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes labelled
T5051 17890-17891 CHEBI_SO_EXT:base denotes b
T5052 17897-17902 PR_EXT:P23940 denotes BamHI
T5053 17903-17910 SO_EXT:0001026 denotes genomic
T5054 17946-17951 SO_EXT:0000147 denotes exons
T5175 17962-17970 GO:0042571 denotes Antibody
T5176 17962-17981 GO_EXT:antibody_or_immunoglobulin_biosynthesis_or_production denotes Antibody production
T5177 17982-17985 CHEBI_SO_EXT:histidine denotes His
T5178 17986-17992 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes tagged
T5179 17997-18000 PR_EXT:000010228 denotes MBP
T5180 18001-18006 SO_EXT:sequence_fusion_process denotes fused
T5181 18007-18018 GO_SO_EXT:sequence_rearrangement_process denotes recombinant
T5182 18019-18026 CHEBI_PR_EXT:protein denotes protein
T5183 18038-18049 GO:0005737 denotes cytoplasmic
T5184 18050-18056 SO_EXT:0000417 denotes domain
T5185 18065-18070 NCBITaxon:9606 denotes human
T5186 18071-18077 PR_EXT:000003718 denotes ADAM22
T5187 18078-18085 SO_EXT:0001060 denotes isoform
T5188 18112-18123 CHEBI_SO_EXT:amino_acid denotes amino acids
T5189 18161-18164 CHEBI_SO_EXT:histidine denotes His
T5190 18165-18171 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes tagged
T5191 18172-18178 PR_EXT:000003718 denotes ADAM22
T5192 18182-18189 CHEBI_PR_EXT:protein denotes protein
T5193 18206-18215 GO_EXT:macromolecule_denaturation denotes denatured
T5194 18232-18234 CHEBI_EXT:nickel denotes Ni
T5195 18235-18238 CHEBI:44557 denotes NTA
T5196 18293-18300 CHEBI_PR_EXT:protein denotes protein
T5197 18352-18360 CHEBI_EXT:60809 denotes adjuvant
T5198 18385-18392 CHEBI:60004 denotes mixture
T5199 18422-18429 NCBITaxon:9986 denotes rabbits
T5200 18439-18444 UBERON:0001977 denotes serum
T5201 18464-18467 CHEBI_SO_EXT:histidine denotes His
T5202 18468-18474 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes tagged
T5203 18475-18481 PR_EXT:000003718 denotes ADAM22
T5204 18485-18492 CHEBI_PR_EXT:protein denotes protein
T5205 18512-18515 PR_EXT:000010228 denotes MBP
T5206 18516-18521 SO_EXT:sequence_fusion_process denotes fused
T5207 18522-18528 PR_EXT:000003718 denotes ADAM22
T5208 18532-18539 CHEBI_PR_EXT:protein denotes protein
T5209 18540-18547 MOP:0000779 denotes coupled
T5210 18591-18596 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes bound
T5211 18597-18607 GO:0042571 denotes antibodies
T5212 18636-18641 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes bound
T5213 18642-18652 GO:0042571 denotes antibodies
T5214 18677-18684 CHEBI_SO_EXT:glycine denotes glycine
T5215 18751-18759 GO:0042571 denotes antibody
T5216 18766-18771 NCBITaxon:9606 denotes human
T5217 18776-18781 NCBITaxon:10088 denotes mouse
T5218 18782-18788 PR_EXT:000003718 denotes ADAM22
T5219 18789-18797 CHEBI_PR_EXT:protein denotes proteins
T5220 18865-18873 GO:0042571 denotes antibody
T5221 18927-18932 NCBITaxon:10088 denotes mouse
T5222 18933-18940 UBERON:0000479 denotes tissues
T5468 18980-18986 PR_EXT:000003718 denotes ADAM22
T5469 18987-18994 CHEBI_PR_EXT:protein denotes protein
T5470 19002-19008 PR_EXT:000003718 denotes Adam22
T5471 19009-19010 SO_EXT:sequence_nullness_or_absence denotes -
T5472 19011-19012 SO_EXT:sequence_nullness_or_absence denotes -
T5473 19013-19017 NCBITaxon:10088 denotes mice
T5474 19071-19081 UBERON:0002037 denotes cerebellum
T5475 19108-19113 NCBITaxon:10088 denotes mouse
T5476 19122-19130 SO_EXT:genotype_or_entity_with_genotype denotes genotype
T5477 19178-19182 CL_GO_EXT:cell denotes cell
T5478 19178-19188 GO:0019835 denotes cell lysis
T5479 19189-19195 CHEBI_CHMO_EXT:buffer_solution denotes buffer
T5480 19203-19207 CHEBI:9754 denotes Tris
T5481 19208-19211 CHEBI:17883 denotes HCl
T5482 19228-19232 CHEBI:26710 denotes NaCl
T5483 19237-19242 CHEBI:63016 denotes NP-40
T5484 19253-19272 CHEBI_GO_EXT:peptidase_or_protease_or_proteinase_inhibitor denotes protease inhibitors
T5485 19312-19316 CL_GO_EXT:cell denotes cell
T5486 19380-19383 CHEBI:8984 denotes SDS
T5487 19394-19405 GO_EXT:biological_movement_or_translocation_process denotes transferred
T5488 19411-19425 CHEBI:53325 denotes nitrocellulose
T5489 19480-19488 GO:0042571 denotes antibody
T5490 19501-19512 GO:0005737 denotes cytoplasmic
T5491 19513-19519 SO_EXT:0000417 denotes domain
T5492 19523-19529 PR_EXT:000003718 denotes ADAM22
T5493 19544-19549 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes Bound
T5494 19550-19560 GO:0042571 denotes antibodies
T5495 19582-19593 NCBITaxon:3704 denotes horseradish
T5496 19594-19604 GO_EXT:0004601 denotes peroxidase
T5497 19605-19613 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes labelled
T5498 19621-19629 GO:0042571 denotes antibody
T5681 19711-19718 _FRAGMENT denotes Primary
T5682 19727-19731 CL:0000001 denotes cell
T5683 19719-19731 CL:0002573 denotes Schwann cell
T5684 19727-19731 CL_GO_EXT:cell denotes cell
T5685 19740-19747 _FRAGMENT denotes Primary
T5686 19756-19761 CL:0000001 denotes cells
T5687 19748-19761 CL:0002573 denotes Schwann cells
T5688 19756-19761 CL_GO_EXT:cell denotes cells
T5689 19801-19805 NCBITaxon:10088 denotes mice
T5690 19877-19891 UBERON:0001322 denotes sciatic nerves
T5691 19990-19997 CHEBI:17234 denotes glucose
T5692 20076-20085 CHEBI:2682 denotes fungizone
T5693 20111-20120 CHEBI:42471 denotes forskolin
T5694 20157-20168 GO_SO_EXT:sequence_rearrangement_process denotes recombinant
T5695 20227-20241 UBERON:0001322 denotes sciatic nerves
T5696 20450-20455 CL_GO_EXT:cell denotes cells
T5697 20520-20528 CHEBI_EXT:D_lysine denotes D-Lysine
T5698 20522-20528 CHEBI_SO_EXT:lysine denotes Lysine
T5699 20586-20594 _FRAGMENT denotes cultured
T5700 20603-20608 CL:0000010 denotes cells
T5701 20595-20608 CL:0002573 denotes Schwann cells
T5702 20603-20608 CL_GO_EXT:cell denotes cells
T5948 20683-20685 GO_EXT:reverse_transcription_or_reverse_transcriptase denotes RT
T5949 20699-20704 UBERON:0007023 denotes Adult
T5950 20713-20717 PATO_UBERON_EXT:male_or_bearer_of_maleness denotes male
T5951 20718-20722 NCBITaxon:10088 denotes mice
T5952 20754-20758 CHEBI_SO_EXT:RNA denotes RNAs
T5953 20777-20787 UBERON:0002037 denotes cerebellum
T5954 20789-20800 UBERON:0002240 denotes spinal cord
T5955 20802-20815 UBERON:0001322 denotes sciatic nerve
T5956 20817-20820 UBERON:0000044 denotes DRG
T5957 20825-20833 _FRAGMENT denotes cultured
T5958 20842-20847 CL:0000010 denotes cells
T5959 20834-20847 CL:0002573 denotes Schwann cells
T5960 20842-20847 CL_GO_EXT:cell denotes cells
T5961 20926-20928 GO_EXT:reverse_transcription_or_reverse_transcriptase denotes RT
T5962 20965-20971 SO_EXT:0000112 denotes primer
T5963 21087-21090 CHEBI_SO_EXT:DNA denotes DNA
T5964 21087-21101 GO_EXT:0034061 denotes DNA polymerase
T5965 21126-21132 PR_EXT:000003718 denotes ADAM22
T5966 21142-21149 SO_EXT:0000112 denotes primers
T5967 21161-21169 GO:0008380 denotes splicing
T5968 21161-21178 SO_EXT:alternative_splice_variant denotes splicing variants
T5969 21186-21197 GO:0005737 denotes cytoplasmic
T5970 21198-21204 SO_EXT:0000417 denotes domain
T5971 21208-21222 SO_EXT:0000121 denotes forward primer
T5972 21239-21247 SO_EXT:sequence_upstreamness denotes upstream
T5973 21260-21268 GO:0016020 denotes membrane
T5974 21269-21275 SO_EXT:0000417 denotes domain
T5975 21282-21296 SO_EXT:0000132 denotes reverse primer
T5976 21324-21328 SO_EXT:0000147 denotes exon
T5977 21334-21341 SO_EXT:0000112 denotes primers
T5978 21384-21391 SO:0001030 denotes forward
T5979 21434-21441 SO:0001031 denotes reverse
T6188 21491-21495 NCBITaxon:10088 denotes Mice
T6189 21501-21513 CHEBI_EXT:anaesthetic_process denotes anesthetized
T6190 21519-21530 CHEBI:35702 denotes ethyl ether
T6191 21538-21542 UBERON_EXT:body denotes body
T6192 21559-21575 CHEBI_EXT:paraformaldehye denotes paraformaldehyde
T6193 21576-21590 CHEBI:64276 denotes glutaraldehyde
T6194 21591-21599 CHEBI:75958 denotes solution
T6195 21612-21619 CHEBI:28304 denotes heparin
T6196 21629-21635 CHEBI_EXT:saline_solution denotes saline
T6197 21651-21665 UBERON:0001322 denotes Sciatic nerves
T6198 21667-21684 UBERON:0001645 denotes trigeminal nerves
T6199 21686-21691 UBERON:0000955 denotes brain
T6200 21696-21707 UBERON:0002240 denotes spinal cord
T6201 21746-21754 CHEBI_CHMO_EXT:buffer_process denotes buffered
T6202 21755-21763 CHEBI:16842 denotes formalin
T6203 21769-21780 UBERON:0002240 denotes spinal cord
T6204 21791-21796 UBERON:0001021 denotes nerve
T6205 21879-21886 CHEBI:16236 denotes ethanol
T6206 22035-22043 CHEBI:10545 denotes electron
T6207 22153-22160 CHEBI:16236 denotes ethanol
T6208 22170-22174 CHEBI_EXT:lead denotes lead
T6209 22175-22182 CHEBI_EXT:citrate denotes citrate
T6210 22203-22211 CHEBI:10545 denotes electron
T6211 22277-22283 NCBITaxon:33208 denotes Animal
T6398 22371-22383 CHEBI:9750 denotes Triton X-100
T6399 22449-22457 CHEBI:75958 denotes solution
T6400 22486-22496 GO:0042571 denotes antibodies
T6401 22558-22563 NCBITaxon:10088 denotes mouse
T6402 22569-22583 PR_EXT:000004967 denotes calbindin 28 K
T6403 22611-22616 NCBITaxon:10088 denotes mouse
T6404 22622-22625 PR_EXT:000010228 denotes MBP
T6405 22683-22688 NCBITaxon:10088 denotes mouse
T6406 22694-22699 PR_EXT:000006252 denotes Neu N
T6407 22719-22725 NCBITaxon:9986 denotes rabbit
T6408 22762-22768 GO:0005634 denotes Nuclei
T6409 22802-22806 CHEBI:51231 denotes DAPI
T6410 22816-22819 PR_EXT:000010228 denotes MBP
T6411 22859-22866 CHEBI_SO_EXT:epitope denotes epitope
T6412 22890-22897 CHEBI_EXT:citrate denotes citrate
T6413 22898-22904 CHEBI_CHMO_EXT:buffer_solution denotes buffer
T6414 22963-22973 GO:0042571 denotes antibodies
T6415 22975-22978 CHEBI:37987 denotes Cy3
T6416 22979-22986 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes labeled
T6417 22987-22993 NCBITaxon:9793 denotes donkey
T6418 22999-23005 NCBITaxon:9986 denotes rabbit
T6419 23006-23009 GO:0071735 denotes IgG
T6420 23044-23048 CHEBI:37926 denotes FITC
T6421 23049-23057 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes labelled
T6422 23058-23064 NCBITaxon:9793 denotes donkey
T6423 23070-23075 NCBITaxon:10088 denotes mouse
T6424 23076-23079 GO:0071735 denotes IgG
T6663 23228-23231 CHEBI_SO_EXT:RNA denotes RNA
T6664 23240-23253 GO:0097617 denotes hybridisation
T6665 23262-23275 GO:0097617 denotes hybridisation
T6666 23318-23321 CHEBI:37983 denotes 35S
T6667 23322-23330 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes labelled
T6668 23331-23341 CHEBI_SO_EXT:riboprobe denotes RNA probes
T6669 23356-23358 SO_EXT:0000028 denotes bp
T6670 23362-23367 NCBITaxon:10088 denotes mouse
T6671 23368-23374 PR_EXT:000003718 denotes ADAM22
T6672 23375-23379 SO_EXT:cDNA denotes cDNA
T6673 23406-23420 SO_EXT:0000318 denotes initiating ATG
T6674 23426-23432 SO_EXT:sequence_cloning_process denotes cloned
T6675 23491-23497 SO_EXT:0000440 denotes vector
T6676 23511-23519 SO_EXT:0000155 denotes plasmids
T6677 23544-23553 SO:0000077 denotes antisense
T6678 23554-23562 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes labelled
T6679 23563-23573 CHEBI_SO_EXT:riboprobe denotes RNA probes
T6680 23592-23594 NCBITaxon:10760 denotes T7
T6681 23592-23609 PR_EXT:P00573 denotes T7 RNA polymerase
T6682 23595-23598 CHEBI_SO_EXT:RNA denotes RNA
T6683 23595-23609 GO_EXT:0034062 denotes RNA polymerase
T6684 23616-23619 CHEBI:37983 denotes 35S
T6685 23620-23623 CHEBI_EXT:UTP denotes UTP
T6686 23632-23637 UBERON:0000955 denotes brain
T6687 23642-23653 UBERON:0002240 denotes spinal cord
T6688 23679-23685 PATO_UBERON_EXT:female_or_bearer_of_femaleness denotes female
T6689 23686-23690 NCBITaxon:10088 denotes mice
T6690 23733-23746 GO:0097617 denotes hybridisation
T6691 23748-23753 GO_EXT:0004540 denotes RNase
R666 T1206 T1205 _lexicallyChainedTo protein,domain of
R1058 T1954 T1953 _lexicallyChainedTo Schwann cells,proliferation of
R1566 T2691 T2690 _lexicallyChainedTo cells,cultured primary
R1567 T2698 T2697 _lexicallyChainedTo cells,cultured
R2117 T3629 T3628 _lexicallyChainedTo nerves,sciatic
R2118 T3654 T3653 _lexicallyChainedTo proliferation,Schwann cell
R3017 T4821 T4820 _lexicallyChainedTo cells,embryonic stem
R3018 T4821 T4822 _lexicallyChainedTo cells,ES
R3554 T5682 T5681 _lexicallyChainedTo cell,Primary
R3555 T5686 T5685 _lexicallyChainedTo cells,Primary
R3556 T5700 T5699 _lexicallyChainedTo cells,cultured
R3754 T5958 T5957 _lexicallyChainedTo cells,cultured

craft-sa-dev

Id Subject Object Predicate Lexical cue
T85 0-6 NN denotes Ataxia
T86 0-68 sentence denotes Ataxia and peripheral nerve hypomyelination in ADAM22-deficient mice
T87 7-10 CC denotes and
T88 11-21 JJ denotes peripheral
T90 22-27 NN denotes nerve
T91 44-46 IN denotes in
T92 47-53 NN denotes ADAM22
T93 54-63 JJ denotes deficient
T94 53-54 HYPH denotes -
T95 64-68 NNS denotes mice
T96 68-69 sentence denotes
T97 79-89 sentence denotes Background
T98 79-89 NN denotes Background
T99 89-212 sentence denotes ADAM22 is a member of the ADAM gene family, but the fact that it is expressed only in the nervous systems makes it unique.
T100 90-96 NN denotes ADAM22
T101 97-99 VBZ denotes is
T102 100-101 DT denotes a
T103 102-108 NN denotes member
T104 109-111 IN denotes of
T105 112-115 DT denotes the
T106 126-132 NN denotes family
T107 116-120 NN denotes ADAM
T108 121-125 NN denotes gene
T109 132-134 , denotes ,
T110 134-137 CC denotes but
T111 138-141 DT denotes the
T112 142-146 NN denotes fact
T113 196-201 VBZ denotes makes
T114 147-151 IN denotes that
T115 158-167 VBN denotes expressed
T116 152-154 PRP denotes it
T117 155-157 VBZ denotes is
T118 168-172 RB denotes only
T119 173-175 IN denotes in
T120 176-179 DT denotes the
T121 188-195 NNS denotes systems
T122 180-187 JJ denotes nervous
T123 202-204 PRP denotes it
T124 205-211 JJ denotes unique
T125 211-212 . denotes .
T126 212-359 sentence denotes ADAM22's sequence similarity to other ADAMs suggests it to be an integrin binder and thus to have a role in cell-cell or cell-matrix interactions.
T127 213-219 NN denotes ADAM22
T128 231-241 NN denotes similarity
T129 219-221 POS denotes 's
T130 222-230 NN denotes sequence
T131 257-265 VBZ denotes suggests
T132 242-244 IN denotes to
T133 245-250 JJ denotes other
T134 251-256 NNS denotes ADAMs
T135 266-268 PRP denotes it
T136 272-274 VB denotes be
T137 269-271 TO denotes to
T138 275-277 DT denotes an
T139 287-293 NN denotes binder
T140 278-286 NN denotes integrin
T141 294-297 CC denotes and
T142 298-302 RB denotes thus
T143 306-310 VB denotes have
T144 303-305 TO denotes to
T145 311-312 DT denotes a
T146 313-317 NN denotes role
T147 318-320 IN denotes in
T148 321-325 NN denotes cell
T149 326-330 NN denotes cell
T150 325-326 HYPH denotes -
T151 346-358 NNS denotes interactions
T152 331-333 CC denotes or
T153 334-338 NN denotes cell
T154 339-345 NN denotes matrix
T155 338-339 HYPH denotes -
T156 358-359 . denotes .
T157 359-472 sentence denotes To elucidate the physiological functions of ADAM22, we employed gene targeting to generate ADAM22 knockout mice.
T158 360-362 TO denotes To
T159 363-372 VB denotes elucidate
T160 415-423 VBD denotes employed
T161 373-376 DT denotes the
T162 391-400 NNS denotes functions
T163 377-390 JJ denotes physiological
T164 401-403 IN denotes of
T165 404-410 NN denotes ADAM22
T166 410-412 , denotes ,
T167 412-414 PRP denotes we
T168 424-428 NN denotes gene
T169 429-438 NN denotes targeting
T170 439-441 TO denotes to
T171 442-450 VB denotes generate
T172 451-457 NN denotes ADAM22
T173 458-466 NN denotes knockout
T174 467-471 NNS denotes mice
T175 471-472 . denotes .
T176 472-481 sentence denotes Results
T177 474-481 NNS denotes Results
T178 481-593 sentence denotes ADAM22-deficient mice were produced in a good accordance with the Mendelian ratio and appeared normal at birth.
T179 482-488 NN denotes ADAM22
T180 489-498 JJ denotes deficient
T181 488-489 HYPH denotes -
T182 499-503 NNS denotes mice
T183 509-517 VBN denotes produced
T184 504-508 VBD denotes were
T185 518-520 IN denotes in
T186 521-522 DT denotes a
T187 528-538 NN denotes accordance
T188 523-527 JJ denotes good
T189 539-543 IN denotes with
T190 544-547 DT denotes the
T191 558-563 NN denotes ratio
T192 548-557 JJ denotes Mendelian
T193 564-567 CC denotes and
T194 568-576 VBD denotes appeared
T195 577-583 JJ denotes normal
T196 584-586 IN denotes at
T197 587-592 NN denotes birth
T198 592-593 . denotes .
T199 593-707 sentence denotes After one week, severe ataxia was observed, and all homozygotes died before weaning, probably due to convulsions.
T200 594-599 IN denotes After
T201 628-636 VBN denotes observed
T202 600-603 CD denotes one
T203 604-608 NN denotes week
T204 608-610 , denotes ,
T205 610-616 JJ denotes severe
T206 617-623 NN denotes ataxia
T207 624-627 VBD denotes was
T208 636-638 , denotes ,
T209 638-641 CC denotes and
T210 642-645 DT denotes all
T211 646-657 NNS denotes homozygotes
T212 658-662 VBD denotes died
T213 663-669 IN denotes before
T214 670-677 NN denotes weaning
T215 677-679 , denotes ,
T216 679-687 RB denotes probably
T217 688-691 IN denotes due
T218 692-694 IN denotes to
T219 695-706 NNS denotes convulsions
T220 706-707 . denotes .
T221 707-892 sentence denotes No major histological abnormalities were detected in the cerebral cortex or cerebellum of the homozygous mutants; however, marked hypomyelination of the peripheral nerves was observed.
T222 708-710 DT denotes No
T223 730-743 NNS denotes abnormalities
T224 711-716 JJ denotes major
T225 717-729 JJ denotes histological
T226 749-757 VBN denotes detected
T227 744-748 VBD denotes were
T228 883-891 VBN denotes observed
T229 758-760 IN denotes in
T230 761-764 DT denotes the
T231 774-780 NN denotes cortex
T232 765-773 JJ denotes cerebral
T233 781-783 CC denotes or
T234 784-794 NN denotes cerebellum
T235 795-797 IN denotes of
T236 798-801 DT denotes the
T237 813-820 NNS denotes mutants
T238 802-812 JJ denotes homozygous
T239 820-821 : denotes ;
T240 822-829 RB denotes however
T241 829-831 , denotes ,
T242 831-837 JJ denotes marked
T243 838-853 NN denotes hypomyelination
T244 854-856 IN denotes of
T245 857-860 DT denotes the
T246 872-878 NNS denotes nerves
T247 861-871 JJ denotes peripheral
T248 879-882 VBD denotes was
T249 891-892 . denotes .
T250 892-904 sentence denotes Conclusion
T251 894-904 NN denotes Conclusion
T252 904-1023 sentence denotes The results of our study demonstrate that ADAM22 is closely involved in the correct functioning of the nervous system.
T253 905-908 DT denotes The
T254 909-916 NNS denotes results
T255 930-941 VBP denotes demonstrate
T256 917-919 IN denotes of
T257 920-923 PRP$ denotes our
T258 924-929 NN denotes study
T259 942-946 IN denotes that
T260 965-973 VBN denotes involved
T261 947-953 NN denotes ADAM22
T262 954-956 VBZ denotes is
T263 957-964 RB denotes closely
T264 974-976 IN denotes in
T265 977-980 DT denotes the
T266 989-1000 NN denotes functioning
T267 981-988 JJ denotes correct
T268 1001-1003 IN denotes of
T269 1004-1007 DT denotes the
T270 1016-1022 NN denotes system
T271 1008-1015 JJ denotes nervous
T272 1022-1023 . denotes .
T273 1023-1173 sentence denotes Further analysis of ADAM22 will provide clues to understanding the mechanisms of human diseases such as epileptic seizures and peripheral neuropathy.
T274 1024-1031 JJ denotes Further
T275 1032-1040 NN denotes analysis
T276 1056-1063 VB denotes provide
T277 1041-1043 IN denotes of
T278 1044-1050 NN denotes ADAM22
T279 1051-1055 MD denotes will
T280 1064-1069 NNS denotes clues
T281 1070-1072 IN denotes to
T282 1073-1086 VBG denotes understanding
T283 1087-1090 DT denotes the
T284 1091-1101 NNS denotes mechanisms
T285 1102-1104 IN denotes of
T286 1105-1110 JJ denotes human
T287 1111-1119 NNS denotes diseases
T288 1120-1124 JJ denotes such
T289 1125-1127 IN denotes as
T290 1128-1137 JJ denotes epileptic
T291 1138-1146 NNS denotes seizures
T292 1147-1150 CC denotes and
T293 1151-1161 JJ denotes peripheral
T294 1162-1172 NN denotes neuropathy
T295 1172-1173 . denotes .
T593 1186-1190 NN denotes ADAM
T594 1227-1229 VBZ denotes is
T595 1191-1192 -LRB- denotes (
T597 1194-1205 NN denotes Disintegrin
T598 1206-1209 CC denotes And
T599 1210-1225 NN denotes Metalloprotease
T600 1225-1226 -RRB- denotes )
T601 1230-1231 DT denotes a
T602 1232-1238 NN denotes family
T603 1239-1241 IN denotes of
T604 1242-1250 NN denotes membrane
T605 1251-1259 VBG denotes spanning
T606 1250-1251 HYPH denotes -
T607 1273-1281 NN denotes proteins
T608 1260-1272 JJ denotes multi-domain
T609 1282-1292 VBG denotes containing
T610 1293-1294 DT denotes a
T611 1318-1324 NN denotes domain
T612 1295-1312 NN denotes metalloproteinase
T613 1313-1317 JJ denotes like
T614 1312-1313 HYPH denotes -
T615 1325-1328 CC denotes and
T616 1329-1330 DT denotes a
T617 1348-1354 NN denotes domain
T618 1331-1342 NN denotes disintegrin
T619 1343-1347 JJ denotes like
T620 1342-1343 HYPH denotes -
T621 1354-1355 . denotes .
T622 1355-1418 sentence denotes Currently, more than 30 ADAMs have been identified in mammals.
T623 1356-1365 RB denotes Currently
T624 1396-1406 VBN denotes identified
T625 1365-1367 , denotes ,
T626 1367-1371 JJR denotes more
T627 1377-1379 CD denotes 30
T628 1372-1376 IN denotes than
T629 1380-1385 NNS denotes ADAMs
T630 1386-1390 VBP denotes have
T631 1391-1395 VBN denotes been
T632 1407-1409 IN denotes in
T633 1410-1417 NNS denotes mammals
T634 1417-1418 . denotes .
T635 1418-1537 sentence denotes Their biological activities implicate ADAMs in fertilization, myogenesis and neurogenesis by proteolysis and adhesion.
T636 1419-1424 PRP$ denotes Their
T637 1436-1446 NNS denotes activities
T638 1425-1435 JJ denotes biological
T639 1447-1456 VBP denotes implicate
T640 1457-1462 NNS denotes ADAMs
T641 1463-1465 IN denotes in
T642 1466-1479 NN denotes fertilization
T643 1479-1481 , denotes ,
T644 1481-1491 NN denotes myogenesis
T645 1492-1495 CC denotes and
T646 1496-1508 NN denotes neurogenesis
T647 1509-1511 IN denotes by
T648 1512-1523 NN denotes proteolysis
T649 1524-1527 CC denotes and
T650 1528-1536 NN denotes adhesion
T651 1536-1537 . denotes .
T652 1537-1686 sentence denotes Some types of ADAM are catalytically active metalloproteases and shed the extracellular domains of membrane-bound growth factors or receptors [1,2].
T653 1538-1542 DT denotes Some
T654 1543-1548 NNS denotes types
T655 1557-1560 VBP denotes are
T656 1549-1551 IN denotes of
T657 1552-1556 NN denotes ADAM
T658 1561-1574 RB denotes catalytically
T659 1575-1581 JJ denotes active
T660 1582-1598 NNS denotes metalloproteases
T661 1599-1602 CC denotes and
T662 1603-1607 VBP denotes shed
T663 1608-1611 DT denotes the
T664 1626-1633 NNS denotes domains
T665 1612-1625 JJ denotes extracellular
T666 1634-1636 IN denotes of
T667 1637-1645 NN denotes membrane
T668 1646-1651 VBN denotes bound
T669 1645-1646 HYPH denotes -
T670 1659-1666 NNS denotes factors
T671 1652-1658 NN denotes growth
T672 1667-1669 CC denotes or
T673 1670-1679 NNS denotes receptors
T674 1680-1681 -LRB- denotes [
T675 1683-1684 CD denotes 2
T676 1681-1682 CD denotes 1
T677 1682-1683 , denotes ,
T678 1684-1685 -RRB- denotes ]
T679 1685-1686 . denotes .
T680 1686-1910 sentence denotes For example, ADAM17 (TACE) has been shown to cleave several substrates, including tumour necrosis factor alpha[3,4], heparin-binding epidermal growth factor-like growth factor [6,7] and transforming growth factor alpha [8].
T681 1687-1690 IN denotes For
T682 1723-1728 VBN denotes shown
T683 1691-1698 NN denotes example
T684 1698-1700 , denotes ,
T685 1700-1706 NN denotes ADAM17
T686 1707-1708 -LRB- denotes (
T687 1708-1712 NN denotes TACE
T688 1712-1713 -RRB- denotes )
T689 1714-1717 VBZ denotes has
T690 1718-1722 VBN denotes been
T691 1729-1731 TO denotes to
T692 1732-1738 VB denotes cleave
T693 1739-1746 JJ denotes several
T694 1747-1757 NNS denotes substrates
T695 1757-1759 , denotes ,
T696 1759-1768 VBG denotes including
T697 1769-1775 NN denotes tumour
T698 1792-1797 NN denotes alpha
T699 1776-1784 NN denotes necrosis
T700 1785-1791 NN denotes factor
T701 1797-1798 -LRB- denotes [
T702 1800-1801 CD denotes 4
T703 1798-1799 CD denotes 3
T704 1799-1800 , denotes ,
T705 1801-1802 -RRB- denotes ]
T706 1802-1804 , denotes ,
T707 1804-1811 NN denotes heparin
T708 1812-1819 VBG denotes binding
T709 1811-1812 HYPH denotes -
T710 1856-1862 NN denotes factor
T711 1820-1829 JJ denotes epidermal
T712 1837-1843 NN denotes factor
T713 1830-1836 NN denotes growth
T714 1844-1848 JJ denotes like
T715 1843-1844 HYPH denotes -
T716 1849-1855 NN denotes growth
T717 1863-1864 -LRB- denotes [
T718 1866-1867 CD denotes 7
T719 1864-1865 CD denotes 6
T720 1865-1866 , denotes ,
T721 1867-1868 -RRB- denotes ]
T722 1869-1872 CC denotes and
T723 1873-1885 VBG denotes transforming
T724 1900-1905 NN denotes alpha
T725 1886-1892 NN denotes growth
T726 1893-1899 NN denotes factor
T727 1906-1907 -LRB- denotes [
T728 1907-1908 CD denotes 8
T729 1908-1909 -RRB- denotes ]
T730 1909-1910 . denotes .
T731 1910-2060 sentence denotes Studies of ADAM17-null mice have revealed that ADAM17 is critical in embryogenesis and plays an essential role in the supply of growth factors [6,8].
T732 1911-1918 NNS denotes Studies
T733 1944-1952 VBN denotes revealed
T734 1919-1921 IN denotes of
T735 1922-1928 NN denotes ADAM17
T736 1929-1933 JJ denotes null
T737 1928-1929 HYPH denotes -
T738 1934-1938 NNS denotes mice
T739 1939-1943 VBP denotes have
T740 1953-1957 IN denotes that
T741 1965-1967 VBZ denotes is
T742 1958-1964 NN denotes ADAM17
T743 1968-1976 JJ denotes critical
T744 1977-1979 IN denotes in
T745 1980-1993 NN denotes embryogenesis
T746 1994-1997 CC denotes and
T747 1998-2003 VBZ denotes plays
T748 2004-2006 DT denotes an
T749 2017-2021 NN denotes role
T750 2007-2016 JJ denotes essential
T751 2022-2024 IN denotes in
T752 2025-2028 DT denotes the
T753 2029-2035 NN denotes supply
T754 2036-2038 IN denotes of
T755 2039-2045 NN denotes growth
T756 2046-2053 NNS denotes factors
T757 2054-2055 -LRB- denotes [
T758 2057-2058 CD denotes 8
T759 2055-2056 CD denotes 6
T760 2056-2057 , denotes ,
T761 2058-2059 -RRB- denotes ]
T762 2059-2060 . denotes .
T763 2060-2176 sentence denotes ADAMs are also involved in cell-cell or cell-matrix adhesion through their interaction with integrins or syndecans.
T764 2061-2066 NNS denotes ADAMs
T765 2076-2084 VBN denotes involved
T766 2067-2070 VBP denotes are
T767 2071-2075 RB denotes also
T768 2085-2087 IN denotes in
T769 2088-2092 NN denotes cell
T770 2093-2097 NN denotes cell
T771 2092-2093 HYPH denotes -
T772 2113-2121 NN denotes adhesion
T773 2098-2100 CC denotes or
T774 2101-2105 NN denotes cell
T775 2106-2112 NN denotes matrix
T776 2105-2106 HYPH denotes -
T777 2122-2129 IN denotes through
T778 2130-2135 PRP$ denotes their
T779 2136-2147 NN denotes interaction
T780 2148-2152 IN denotes with
T781 2153-2162 NNS denotes integrins
T782 2163-2165 CC denotes or
T783 2166-2175 NNS denotes syndecans
T784 2175-2176 . denotes .
T785 2176-2268 sentence denotes More than 10 ADAMs have been shown to support integrin-mediated cell adhesion in vitro [9].
T786 2177-2181 JJR denotes More
T787 2187-2189 CD denotes 10
T788 2182-2186 IN denotes than
T789 2190-2195 NNS denotes ADAMs
T790 2206-2211 VBN denotes shown
T791 2196-2200 VBP denotes have
T792 2201-2205 VBN denotes been
T793 2212-2214 TO denotes to
T794 2215-2222 VB denotes support
T795 2223-2231 NN denotes integrin
T796 2232-2240 VBN denotes mediated
T797 2231-2232 HYPH denotes -
T798 2246-2254 NN denotes adhesion
T799 2241-2245 NN denotes cell
T800 2255-2257 FW denotes in
T801 2258-2263 FW denotes vitro
T802 2264-2265 -LRB- denotes [
T803 2265-2266 CD denotes 9
T804 2266-2267 -RRB- denotes ]
T805 2267-2268 . denotes .
T806 2268-2405 sentence denotes It has been reported that ADAM2-null and ADAM3-null male mutants are infertile and their spermatozoa lack egg-binding abilities [10-12].
T807 2269-2271 PRP denotes It
T808 2281-2289 VBN denotes reported
T809 2272-2275 VBZ denotes has
T810 2276-2280 VBN denotes been
T811 2290-2294 IN denotes that
T812 2334-2337 VBP denotes are
T813 2295-2300 NN denotes ADAM2
T814 2301-2305 JJ denotes null
T815 2300-2301 HYPH denotes -
T816 2326-2333 NNS denotes mutants
T817 2306-2309 CC denotes and
T818 2310-2315 NN denotes ADAM3
T819 2316-2320 JJ denotes null
T820 2315-2316 HYPH denotes -
T821 2321-2325 JJ denotes male
T822 2338-2347 JJ denotes infertile
T823 2348-2351 CC denotes and
T824 2352-2357 PRP$ denotes their
T825 2358-2369 NNS denotes spermatozoa
T826 2370-2374 VBP denotes lack
T827 2375-2378 NN denotes egg
T828 2379-2386 VBG denotes binding
T829 2378-2379 HYPH denotes -
T830 2387-2396 NNS denotes abilities
T831 2397-2398 -LRB- denotes [
T832 2398-2400 CD denotes 10
T833 2400-2401 SYM denotes -
T834 2401-2403 CD denotes 12
T835 2403-2404 -RRB- denotes ]
T836 2404-2405 . denotes .
T837 2405-2527 sentence denotes Both ADAM2 and ADAM3 are not metalloproteases because they lack catalytic site sequences in their metalloprotease domain.
T838 2406-2410 CC denotes Both
T839 2411-2416 NN denotes ADAM2
T840 2427-2430 VBP denotes are
T841 2417-2420 CC denotes and
T842 2421-2426 NN denotes ADAM3
T843 2431-2434 RB denotes not
T844 2435-2451 NNS denotes metalloproteases
T845 2452-2459 IN denotes because
T846 2465-2469 VBP denotes lack
T847 2460-2464 PRP denotes they
T848 2470-2479 JJ denotes catalytic
T849 2485-2494 NNS denotes sequences
T850 2480-2484 NN denotes site
T851 2495-2497 IN denotes in
T852 2498-2503 PRP$ denotes their
T853 2520-2526 NN denotes domain
T854 2504-2519 NN denotes metalloprotease
T855 2526-2527 . denotes .
T856 2527-2630 sentence denotes These studies clearly showed that non-proteinase members of ADAMs also have significant roles in vivo.
T857 2528-2533 DT denotes These
T858 2534-2541 NNS denotes studies
T859 2550-2556 VBD denotes showed
T860 2542-2549 RB denotes clearly
T861 2557-2561 IN denotes that
T862 2599-2603 VBP denotes have
T863 2562-2576 JJ denotes non-proteinase
T864 2577-2584 NNS denotes members
T865 2585-2587 IN denotes of
T866 2588-2593 NNS denotes ADAMs
T867 2594-2598 RB denotes also
T868 2604-2615 JJ denotes significant
T869 2616-2621 NNS denotes roles
T870 2622-2624 FW denotes in
T871 2625-2629 FW denotes vivo
T872 2629-2630 . denotes .
T873 2630-2776 sentence denotes We have reported the findings of ADAM11, ADAM22 and ADAM23 genes and their restricted expression in the human and murine nervous systems [13-15].
T874 2631-2633 PRP denotes We
T875 2639-2647 VBN denotes reported
T876 2634-2638 VBP denotes have
T877 2648-2651 DT denotes the
T878 2652-2660 NNS denotes findings
T879 2661-2663 IN denotes of
T880 2664-2670 NN denotes ADAM11
T881 2690-2695 NNS denotes genes
T882 2670-2672 , denotes ,
T883 2672-2678 NN denotes ADAM22
T884 2679-2682 CC denotes and
T885 2683-2689 NN denotes ADAM23
T886 2696-2699 CC denotes and
T887 2700-2705 PRP$ denotes their
T888 2717-2727 NN denotes expression
T889 2706-2716 JJ denotes restricted
T890 2728-2730 IN denotes in
T891 2731-2734 DT denotes the
T892 2760-2767 NNS denotes systems
T893 2735-2740 JJ denotes human
T894 2741-2744 CC denotes and
T895 2745-2751 JJ denotes murine
T896 2752-2759 JJ denotes nervous
T897 2768-2769 -LRB- denotes [
T898 2769-2771 CD denotes 13
T899 2771-2772 SYM denotes -
T900 2772-2774 CD denotes 15
T901 2774-2775 -RRB- denotes ]
T902 2775-2776 . denotes .
T903 2776-2878 sentence denotes Sequence analysis suggests that they are not metalloproteases, since they all lack a catalytic motif.
T904 2777-2785 NN denotes Sequence
T905 2786-2794 NN denotes analysis
T906 2795-2803 VBZ denotes suggests
T907 2804-2808 IN denotes that
T908 2814-2817 VBP denotes are
T909 2809-2813 PRP denotes they
T910 2818-2821 RB denotes not
T911 2822-2838 NNS denotes metalloproteases
T912 2838-2840 , denotes ,
T913 2840-2845 IN denotes since
T914 2855-2859 VBP denotes lack
T915 2846-2850 PRP denotes they
T916 2851-2854 DT denotes all
T917 2860-2861 DT denotes a
T918 2872-2877 NN denotes motif
T919 2862-2871 JJ denotes catalytic
T920 2877-2878 . denotes .
T921 2878-3128 sentence denotes It has been reported that ADAM23 protein is localised to the cell surface [16], interacts with alpha-v beta-3 integrin heterodimer [17] and the disruption of ADAM23 gene in the mouse results in premature death associated with ataxia and tremor [18].
T922 2879-2881 PRP denotes It
T923 2891-2899 VBN denotes reported
T924 2882-2885 VBZ denotes has
T925 2886-2890 VBN denotes been
T926 2900-2904 IN denotes that
T927 2923-2932 VBN denotes localised
T928 2905-2911 NN denotes ADAM23
T929 2912-2919 NN denotes protein
T930 2920-2922 VBZ denotes is
T931 2933-2935 IN denotes to
T932 2936-2939 DT denotes the
T933 2945-2952 NN denotes surface
T934 2940-2944 NN denotes cell
T935 2953-2954 -LRB- denotes [
T936 2954-2956 CD denotes 16
T937 2956-2957 -RRB- denotes ]
T938 2957-2959 , denotes ,
T939 2959-2968 VBZ denotes interacts
T940 2969-2973 IN denotes with
T941 2974-2979 NN denotes alpha
T942 2980-2981 NN denotes v
T943 2979-2980 HYPH denotes -
T944 2998-3009 NN denotes heterodimer
T945 2982-2986 NN denotes beta
T946 2986-2987 HYPH denotes -
T947 2987-2988 CD denotes 3
T948 2989-2997 NN denotes integrin
T949 3010-3011 -LRB- denotes [
T950 3011-3013 CD denotes 17
T951 3013-3014 -RRB- denotes ]
T952 3015-3018 CC denotes and
T953 3019-3022 DT denotes the
T954 3023-3033 NN denotes disruption
T955 3062-3069 VBZ denotes results
T956 3034-3036 IN denotes of
T957 3037-3043 NN denotes ADAM23
T958 3044-3048 NN denotes gene
T959 3049-3051 IN denotes in
T960 3052-3055 DT denotes the
T961 3056-3061 NN denotes mouse
T962 3070-3072 IN denotes in
T963 3073-3082 JJ denotes premature
T964 3083-3088 NN denotes death
T965 3089-3099 VBN denotes associated
T966 3100-3104 IN denotes with
T967 3105-3111 NN denotes ataxia
T968 3112-3115 CC denotes and
T969 3116-3122 NN denotes tremor
T970 3123-3124 -LRB- denotes [
T971 3124-3126 CD denotes 18
T972 3126-3127 -RRB- denotes ]
T973 3127-3128 . denotes .
T974 3128-3318 sentence denotes Although the cause of death in this mouse is unknown, for these phenotypes, impaired cell-cell or cell-matrix interactions in the nervous system caused by loss of ADAM23 may be responsible.
T975 3129-3137 IN denotes Although
T976 3171-3173 VBZ denotes is
T977 3138-3141 DT denotes the
T978 3142-3147 NN denotes cause
T979 3148-3150 IN denotes of
T980 3151-3156 NN denotes death
T981 3157-3159 IN denotes in
T982 3160-3164 DT denotes this
T983 3165-3170 NN denotes mouse
T984 3303-3305 VB denotes be
T985 3174-3181 JJ denotes unknown
T986 3181-3183 , denotes ,
T987 3183-3186 IN denotes for
T988 3187-3192 DT denotes these
T989 3193-3203 NNS denotes phenotypes
T990 3203-3205 , denotes ,
T991 3205-3213 VBN denotes impaired
T992 3239-3251 NNS denotes interactions
T993 3214-3218 NN denotes cell
T994 3219-3223 NN denotes cell
T995 3218-3219 HYPH denotes -
T996 3224-3226 CC denotes or
T997 3227-3231 NN denotes cell
T998 3232-3238 NN denotes matrix
T999 3231-3232 HYPH denotes -
T1000 3252-3254 IN denotes in
T1001 3255-3258 DT denotes the
T1002 3267-3273 NN denotes system
T1003 3259-3266 JJ denotes nervous
T1004 3274-3280 VBN denotes caused
T1005 3281-3283 IN denotes by
T1006 3284-3288 NN denotes loss
T1007 3289-3291 IN denotes of
T1008 3292-3298 NN denotes ADAM23
T1009 3299-3302 MD denotes may
T1010 3306-3317 JJ denotes responsible
T1011 3317-3318 . denotes .
T1012 3318-3402 sentence denotes ADAM22 and ADAM23 share highly homologous sequences in their extracellular domains.
T1013 3319-3325 NN denotes ADAM22
T1014 3337-3342 VBP denotes share
T1015 3326-3329 CC denotes and
T1016 3330-3336 NN denotes ADAM23
T1017 3343-3349 RB denotes highly
T1018 3350-3360 JJ denotes homologous
T1019 3361-3370 NNS denotes sequences
T1020 3371-3373 IN denotes in
T1021 3374-3379 PRP$ denotes their
T1022 3394-3401 NNS denotes domains
T1023 3380-3393 JJ denotes extracellular
T1024 3401-3402 . denotes .
T1025 3402-3549 sentence denotes Especially, it is evident in their putative integrin binding loop sequences, CR(E/D)AVN(E/D)CD, which is located centre of the disintegrin domain.
T1026 3403-3413 RB denotes Especially
T1027 3418-3420 VBZ denotes is
T1028 3413-3415 , denotes ,
T1029 3415-3417 PRP denotes it
T1030 3421-3428 JJ denotes evident
T1031 3429-3431 IN denotes in
T1032 3432-3437 PRP$ denotes their
T1033 3469-3478 NNS denotes sequences
T1034 3438-3446 JJ denotes putative
T1035 3447-3455 NN denotes integrin
T1036 3456-3463 VBG denotes binding
T1037 3464-3468 NN denotes loop
T1038 3478-3480 , denotes ,
T1039 3480-3482 NN denotes CR
T1040 3495-3497 NN denotes CD
T1041 3482-3483 -LRB- denotes (
T1042 3485-3486 NN denotes D
T1043 3483-3484 NN denotes E
T1044 3484-3485 HYPH denotes /
T1045 3486-3487 -RRB- denotes )
T1046 3487-3490 NN denotes AVN
T1047 3490-3491 -LRB- denotes (
T1048 3493-3494 NN denotes D
T1049 3491-3492 NN denotes E
T1050 3492-3493 HYPH denotes /
T1051 3494-3495 -RRB- denotes )
T1052 3497-3499 , denotes ,
T1053 3499-3504 WDT denotes which
T1054 3508-3515 VBN denotes located
T1055 3505-3507 VBZ denotes is
T1056 3516-3522 RB denotes centre
T1057 3523-3525 IN denotes of
T1058 3526-3529 DT denotes the
T1059 3542-3548 NN denotes domain
T1060 3530-3541 NN denotes disintegrin
T1061 3548-3549 . denotes .
T1062 3549-3687 sentence denotes These findings led us to hypothesize that ADAM22 is an integrin binder and plays an important role in the nervous system, as does ADAM23.
T1063 3550-3555 DT denotes These
T1064 3556-3564 NNS denotes findings
T1065 3565-3568 VBD denotes led
T1066 3569-3571 PRP denotes us
T1067 3572-3574 TO denotes to
T1068 3575-3586 VB denotes hypothesize
T1069 3587-3591 IN denotes that
T1070 3599-3601 VBZ denotes is
T1071 3592-3598 NN denotes ADAM22
T1072 3602-3604 DT denotes an
T1073 3614-3620 NN denotes binder
T1074 3605-3613 NN denotes integrin
T1075 3621-3624 CC denotes and
T1076 3625-3630 VBZ denotes plays
T1077 3631-3633 DT denotes an
T1078 3644-3648 NN denotes role
T1079 3634-3643 JJ denotes important
T1080 3649-3651 IN denotes in
T1081 3652-3655 DT denotes the
T1082 3664-3670 NN denotes system
T1083 3656-3663 JJ denotes nervous
T1084 3670-3672 , denotes ,
T1085 3672-3674 IN denotes as
T1086 3675-3679 VBZ denotes does
T1087 3680-3686 NN denotes ADAM23
T1088 3686-3687 . denotes .
T1089 3687-3792 sentence denotes To determine the physiological functions of ADAM22, we generated and analysed Adam22 gene-targeted mice.
T1090 3688-3690 TO denotes To
T1091 3691-3700 VB denotes determine
T1092 3743-3752 VBD denotes generated
T1093 3701-3704 DT denotes the
T1094 3719-3728 NNS denotes functions
T1095 3705-3718 JJ denotes physiological
T1096 3729-3731 IN denotes of
T1097 3732-3738 NN denotes ADAM22
T1098 3738-3740 , denotes ,
T1099 3740-3742 PRP denotes we
T1100 3753-3756 CC denotes and
T1101 3757-3765 VBD denotes analysed
T1102 3766-3772 NN denotes Adam22
T1103 3787-3791 NNS denotes mice
T1104 3773-3777 NN denotes gene
T1105 3778-3786 VBN denotes targeted
T1106 3777-3778 HYPH denotes -
T1107 3791-3792 . denotes .
T1243 3803-3813 NN denotes Generation
T1244 3814-3816 IN denotes of
T1245 3817-3823 NN denotes ADAM22
T1246 3824-3833 JJ denotes deficient
T1247 3823-3824 HYPH denotes -
T1248 3834-3838 NNS denotes mice
T1249 3838-3947 sentence denotes Mice carrying a targeted mutation in their Adam22 gene were generated by homologous recombination (Fig. 1A).
T1250 3839-3843 NNS denotes Mice
T1251 3899-3908 VBN denotes generated
T1252 3844-3852 VBG denotes carrying
T1253 3853-3854 DT denotes a
T1254 3864-3872 NN denotes mutation
T1255 3855-3863 VBN denotes targeted
T1256 3873-3875 IN denotes in
T1257 3876-3881 PRP$ denotes their
T1258 3889-3893 NN denotes gene
T1259 3882-3888 NN denotes Adam22
T1260 3894-3898 VBD denotes were
T1261 3909-3911 IN denotes by
T1262 3912-3922 JJ denotes homologous
T1263 3923-3936 NN denotes recombination
T1264 3937-3938 -LRB- denotes (
T1265 3943-3945 NN denotes 1A
T1266 3938-3942 NN denotes Fig.
T1267 3945-3946 -RRB- denotes )
T1268 3946-3947 . denotes .
T1269 3947-4024 sentence denotes Correct targeting events were confirmed by Southern blot analysis (Fig. 1B).
T1270 3948-3955 JJ denotes Correct
T1271 3966-3972 NNS denotes events
T1272 3956-3965 VBG denotes targeting
T1273 3978-3987 VBN denotes confirmed
T1274 3973-3977 VBD denotes were
T1275 3988-3990 IN denotes by
T1276 3991-3999 NNP denotes Southern
T1277 4000-4004 NN denotes blot
T1278 4005-4013 NN denotes analysis
T1279 4014-4015 -LRB- denotes (
T1280 4020-4022 NN denotes 1B
T1281 4015-4019 NN denotes Fig.
T1282 4022-4023 -RRB- denotes )
T1283 4023-4024 . denotes .
T1284 4024-4198 sentence denotes Since the termination codon was introduced in exon 8 in the pro-protein domain, only the truncated form of the ADAM22 protein would be synthesized from this targeted allele.
T1285 4025-4030 IN denotes Since
T1286 4057-4067 VBN denotes introduced
T1287 4031-4034 DT denotes the
T1288 4047-4052 NN denotes codon
T1289 4035-4046 NN denotes termination
T1290 4053-4056 VBD denotes was
T1291 4160-4171 VBN denotes synthesized
T1292 4068-4070 IN denotes in
T1293 4071-4075 NN denotes exon
T1294 4076-4077 CD denotes 8
T1295 4078-4080 IN denotes in
T1296 4081-4084 DT denotes the
T1297 4097-4103 NN denotes domain
T1298 4085-4096 JJ denotes pro-protein
T1299 4103-4105 , denotes ,
T1300 4105-4109 RB denotes only
T1301 4124-4128 NN denotes form
T1302 4110-4113 DT denotes the
T1303 4114-4123 VBN denotes truncated
T1304 4129-4131 IN denotes of
T1305 4132-4135 DT denotes the
T1306 4143-4150 NN denotes protein
T1307 4136-4142 NN denotes ADAM22
T1308 4151-4156 MD denotes would
T1309 4157-4159 VB denotes be
T1310 4172-4176 IN denotes from
T1311 4177-4181 DT denotes this
T1312 4191-4197 NN denotes allele
T1313 4182-4190 VBN denotes targeted
T1314 4197-4198 . denotes .
T1315 4198-4430 sentence denotes Because a pro-protein domain is always removed in the mature functional ADAM-proteins by the Furin-like proteases and is thought to be non-functional itself, we considered that this truncated form of ADAM22 protein has no function.
T1316 4199-4206 IN denotes Because
T1317 4238-4245 VBN denotes removed
T1318 4207-4208 DT denotes a
T1319 4221-4227 NN denotes domain
T1320 4209-4220 JJ denotes pro-protein
T1321 4228-4230 VBZ denotes is
T1322 4231-4237 RB denotes always
T1323 4360-4370 VBD denotes considered
T1324 4246-4248 IN denotes in
T1325 4249-4252 DT denotes the
T1326 4276-4284 NN denotes proteins
T1327 4253-4259 JJ denotes mature
T1328 4260-4270 JJ denotes functional
T1329 4271-4275 NN denotes ADAM
T1330 4275-4276 HYPH denotes -
T1331 4285-4287 IN denotes by
T1332 4288-4291 DT denotes the
T1333 4303-4312 NNS denotes proteases
T1334 4292-4297 NN denotes Furin
T1335 4298-4302 JJ denotes like
T1336 4297-4298 HYPH denotes -
T1337 4313-4316 CC denotes and
T1338 4317-4319 VBZ denotes is
T1339 4320-4327 VBN denotes thought
T1340 4328-4330 TO denotes to
T1341 4331-4333 VB denotes be
T1342 4334-4348 JJ denotes non-functional
T1343 4349-4355 PRP denotes itself
T1344 4355-4357 , denotes ,
T1345 4357-4359 PRP denotes we
T1346 4371-4375 IN denotes that
T1347 4414-4417 VBZ denotes has
T1348 4376-4380 DT denotes this
T1349 4391-4395 NN denotes form
T1350 4381-4390 VBN denotes truncated
T1351 4396-4398 IN denotes of
T1352 4399-4405 NN denotes ADAM22
T1353 4406-4413 NN denotes protein
T1354 4418-4420 DT denotes no
T1355 4421-4429 NN denotes function
T1356 4429-4430 . denotes .
T1357 4430-4626 sentence denotes Absence of mature ADAM22 protein in homozygous mutants was confirmed by Western blot analysis using the specific antibody, which recognizes the cytoplasmic domain of the ADAM22 protein (Fig. 1C).
T1358 4431-4438 NN denotes Absence
T1359 4490-4499 VBN denotes confirmed
T1360 4439-4441 IN denotes of
T1361 4442-4448 JJ denotes mature
T1362 4456-4463 NN denotes protein
T1363 4449-4455 NN denotes ADAM22
T1364 4464-4466 IN denotes in
T1365 4467-4477 JJ denotes homozygous
T1366 4478-4485 NNS denotes mutants
T1367 4486-4489 VBD denotes was
T1368 4500-4502 IN denotes by
T1369 4503-4510 NNP denotes Western
T1370 4511-4515 NN denotes blot
T1371 4516-4524 NN denotes analysis
T1372 4525-4530 VBG denotes using
T1373 4531-4534 DT denotes the
T1374 4544-4552 NN denotes antibody
T1375 4535-4543 JJ denotes specific
T1376 4552-4554 , denotes ,
T1377 4554-4559 WDT denotes which
T1378 4560-4570 VBZ denotes recognizes
T1379 4571-4574 DT denotes the
T1380 4587-4593 NN denotes domain
T1381 4575-4586 JJ denotes cytoplasmic
T1382 4594-4596 IN denotes of
T1383 4597-4600 DT denotes the
T1384 4608-4615 NN denotes protein
T1385 4601-4607 NN denotes ADAM22
T1386 4616-4617 -LRB- denotes (
T1387 4622-4624 NN denotes 1C
T1388 4617-4621 NN denotes Fig.
T1389 4624-4625 -RRB- denotes )
T1390 4625-4626 . denotes .
T1391 4626-4776 sentence denotes Homozygous mutants showed no noticeable defects at birth and were indistinguishable from wild-type or heterozygous littermates during the first week.
T1392 4627-4637 JJ denotes Homozygous
T1393 4638-4645 NNS denotes mutants
T1394 4646-4652 VBD denotes showed
T1395 4653-4655 DT denotes no
T1396 4667-4674 NNS denotes defects
T1397 4656-4666 JJ denotes noticeable
T1398 4675-4677 IN denotes at
T1399 4678-4683 NN denotes birth
T1400 4684-4687 CC denotes and
T1401 4688-4692 VBD denotes were
T1402 4693-4710 JJ denotes indistinguishable
T1403 4711-4715 IN denotes from
T1404 4716-4720 JJ denotes wild
T1405 4721-4725 NN denotes type
T1406 4720-4721 HYPH denotes -
T1407 4742-4753 NNS denotes littermates
T1408 4726-4728 CC denotes or
T1409 4729-4741 JJ denotes heterozygous
T1410 4754-4760 IN denotes during
T1411 4761-4764 DT denotes the
T1412 4771-4775 NN denotes week
T1413 4765-4770 JJ denotes first
T1414 4775-4776 . denotes .
T1415 4776-4944 sentence denotes At postnatal day 10 (P10), most of the homozygous mutants were distinguishable by abnormalities such as reduced body weight and uncoordinated movements of their limbs.
T1416 4777-4779 IN denotes At
T1417 4835-4839 VBD denotes were
T1418 4780-4789 JJ denotes postnatal
T1419 4790-4793 NN denotes day
T1420 4794-4796 CD denotes 10
T1421 4797-4798 -LRB- denotes (
T1422 4798-4801 NN denotes P10
T1423 4801-4802 -RRB- denotes )
T1424 4802-4804 , denotes ,
T1425 4804-4808 JJS denotes most
T1426 4809-4811 IN denotes of
T1427 4812-4815 DT denotes the
T1428 4827-4834 NNS denotes mutants
T1429 4816-4826 JJ denotes homozygous
T1430 4840-4855 JJ denotes distinguishable
T1431 4856-4858 IN denotes by
T1432 4859-4872 NNS denotes abnormalities
T1433 4873-4877 JJ denotes such
T1434 4878-4880 IN denotes as
T1435 4881-4888 VBN denotes reduced
T1436 4894-4900 NN denotes weight
T1437 4889-4893 NN denotes body
T1438 4901-4904 CC denotes and
T1439 4905-4918 JJ denotes uncoordinated
T1440 4919-4928 NNS denotes movements
T1441 4929-4931 IN denotes of
T1442 4932-4937 PRP$ denotes their
T1443 4938-4943 NNS denotes limbs
T1444 4943-4944 . denotes .
T1445 4944-5022 sentence denotes After P10, all homozygotes displayed severe ataxia (Fig. 2) and began to die.
T1446 4945-4950 IN denotes After
T1447 4972-4981 VBD denotes displayed
T1448 4951-4954 NN denotes P10
T1449 4954-4956 , denotes ,
T1450 4956-4959 DT denotes all
T1451 4960-4971 NNS denotes homozygotes
T1452 4982-4988 JJ denotes severe
T1453 4989-4995 NN denotes ataxia
T1454 4996-4997 -LRB- denotes (
T1455 4997-5001 NN denotes Fig.
T1456 5002-5003 CD denotes 2
T1457 5003-5004 -RRB- denotes )
T1458 5005-5008 CC denotes and
T1459 5009-5014 VBD denotes began
T1460 5015-5017 TO denotes to
T1461 5018-5021 VB denotes die
T1462 5021-5022 . denotes .
T1463 5022-5174 sentence denotes To measure the survival rate and body weight of each genotype precisely, we backcrossed heterozygous male mutants to C57BL/6 females more than 6 times.
T1464 5023-5025 TO denotes To
T1465 5026-5033 VB denotes measure
T1466 5099-5110 VBD denotes backcrossed
T1467 5034-5037 DT denotes the
T1468 5047-5051 NN denotes rate
T1469 5038-5046 NN denotes survival
T1470 5052-5055 CC denotes and
T1471 5056-5060 NN denotes body
T1472 5061-5067 NN denotes weight
T1473 5068-5070 IN denotes of
T1474 5071-5075 DT denotes each
T1475 5076-5084 NN denotes genotype
T1476 5085-5094 RB denotes precisely
T1477 5094-5096 , denotes ,
T1478 5096-5098 PRP denotes we
T1479 5111-5123 JJ denotes heterozygous
T1480 5129-5136 NNS denotes mutants
T1481 5124-5128 JJ denotes male
T1482 5137-5139 IN denotes to
T1483 5140-5145 NN denotes C57BL
T1484 5148-5155 NNS denotes females
T1485 5145-5146 HYPH denotes /
T1486 5146-5147 CD denotes 6
T1487 5156-5160 JJR denotes more
T1488 5166-5167 CD denotes 6
T1489 5161-5165 IN denotes than
T1490 5168-5173 NNS denotes times
T1491 5173-5174 . denotes .
T1492 5174-5285 sentence denotes The resulting (N 6) heterozygous males and females were intercrossed and the produced offspring were analysed.
T1493 5175-5178 DT denotes The
T1494 5208-5213 NNS denotes males
T1495 5179-5188 VBG denotes resulting
T1496 5189-5190 -LRB- denotes (
T1497 5190-5191 NN denotes N
T1498 5192-5193 CD denotes 6
T1499 5193-5194 -RRB- denotes )
T1500 5195-5207 JJ denotes heterozygous
T1501 5231-5243 VBN denotes intercrossed
T1502 5214-5217 CC denotes and
T1503 5218-5225 NNS denotes females
T1504 5226-5230 VBD denotes were
T1505 5244-5247 CC denotes and
T1506 5248-5251 DT denotes the
T1507 5261-5270 NN denotes offspring
T1508 5252-5260 VBN denotes produced
T1509 5276-5284 VBN denotes analysed
T1510 5271-5275 VBD denotes were
T1511 5284-5285 . denotes .
T1512 5285-5362 sentence denotes The numbers of survivors of each genotype every 5 days are shown in Table 1.
T1513 5286-5289 DT denotes The
T1514 5290-5297 NNS denotes numbers
T1515 5345-5350 VBN denotes shown
T1516 5298-5300 IN denotes of
T1517 5301-5310 NNS denotes survivors
T1518 5311-5313 IN denotes of
T1519 5314-5318 DT denotes each
T1520 5319-5327 NN denotes genotype
T1521 5328-5333 DT denotes every
T1522 5336-5340 NNS denotes days
T1523 5334-5335 CD denotes 5
T1524 5341-5344 VBP denotes are
T1525 5351-5353 IN denotes in
T1526 5354-5359 NN denotes Table
T1527 5360-5361 CD denotes 1
T1528 5361-5362 . denotes .
T1529 5362-5490 sentence denotes At P10, the ratio of each genotype was in close accordance with the Mendelian ratio (20.5% +/+, 55.1% +/-, 24.4% -/- ; n = 78).
T1530 5363-5365 IN denotes At
T1531 5398-5401 VBD denotes was
T1532 5366-5369 NN denotes P10
T1533 5369-5371 , denotes ,
T1534 5371-5374 DT denotes the
T1535 5375-5380 NN denotes ratio
T1536 5381-5383 IN denotes of
T1537 5384-5388 DT denotes each
T1538 5389-5397 NN denotes genotype
T1539 5402-5404 IN denotes in
T1540 5405-5410 JJ denotes close
T1541 5411-5421 NN denotes accordance
T1542 5422-5426 IN denotes with
T1543 5427-5430 DT denotes the
T1544 5441-5446 NN denotes ratio
T1545 5431-5440 JJ denotes Mendelian
T1546 5447-5448 -LRB- denotes (
T1547 5452-5453 NN denotes %
T1548 5448-5452 CD denotes 20.5
T1549 5454-5455 SYM denotes +
T1550 5455-5456 HYPH denotes /
T1551 5456-5457 SYM denotes +
T1552 5457-5459 , denotes ,
T1553 5459-5463 CD denotes 55.1
T1554 5463-5464 NN denotes %
T1555 5465-5466 SYM denotes +
T1556 5466-5467 HYPH denotes /
T1557 5467-5468 SYM denotes -
T1558 5468-5470 , denotes ,
T1559 5470-5474 CD denotes 24.4
T1560 5474-5475 NN denotes %
T1561 5476-5477 SYM denotes -
T1562 5477-5478 HYPH denotes /
T1563 5478-5479 SYM denotes -
T1564 5480-5481 : denotes ;
T1565 5482-5483 NN denotes n
T1566 5486-5488 CD denotes 78
T1567 5484-5485 SYM denotes =
T1568 5488-5489 -RRB- denotes )
T1569 5489-5490 . denotes .
T1570 5490-5556 sentence denotes This result shows that ADAM22 is not essential for embryogenesis.
T1571 5491-5495 DT denotes This
T1572 5496-5502 NN denotes result
T1573 5503-5508 VBZ denotes shows
T1574 5509-5513 IN denotes that
T1575 5521-5523 VBZ denotes is
T1576 5514-5520 NN denotes ADAM22
T1577 5524-5527 RB denotes not
T1578 5528-5537 JJ denotes essential
T1579 5538-5541 IN denotes for
T1580 5542-5555 NN denotes embryogenesis
T1581 5555-5556 . denotes .
T1582 5556-5691 sentence denotes At P10, the average body weight of homozygous mutants was approximately half that of wild-type and heterozygous littermates (Table 2).
T1583 5557-5559 IN denotes At
T1584 5611-5614 VBD denotes was
T1585 5560-5563 NN denotes P10
T1586 5563-5565 , denotes ,
T1587 5565-5568 DT denotes the
T1588 5582-5588 NN denotes weight
T1589 5569-5576 JJ denotes average
T1590 5577-5581 NN denotes body
T1591 5589-5591 IN denotes of
T1592 5592-5602 JJ denotes homozygous
T1593 5603-5610 NNS denotes mutants
T1594 5615-5628 RB denotes approximately
T1595 5629-5633 NN denotes half
T1596 5634-5638 DT denotes that
T1597 5639-5641 IN denotes of
T1598 5642-5646 JJ denotes wild
T1599 5647-5651 NN denotes type
T1600 5646-5647 HYPH denotes -
T1601 5669-5680 NNS denotes littermates
T1602 5652-5655 CC denotes and
T1603 5656-5668 JJ denotes heterozygous
T1604 5681-5682 -LRB- denotes (
T1605 5682-5687 NN denotes Table
T1606 5688-5689 CD denotes 2
T1607 5689-5690 -RRB- denotes )
T1608 5690-5691 . denotes .
T1609 5691-5774 sentence denotes Homozygous mutants died one by one after P10, and all homozygotes died before P20.
T1610 5692-5702 JJ denotes Homozygous
T1611 5703-5710 NNS denotes mutants
T1612 5711-5715 VBD denotes died
T1613 5716-5719 CD denotes one
T1614 5720-5722 IN denotes by
T1615 5723-5726 CD denotes one
T1616 5727-5732 IN denotes after
T1617 5733-5736 NN denotes P10
T1618 5736-5738 , denotes ,
T1619 5738-5741 CC denotes and
T1620 5742-5745 DT denotes all
T1621 5746-5757 NNS denotes homozygotes
T1622 5758-5762 VBD denotes died
T1623 5763-5769 IN denotes before
T1624 5770-5773 NN denotes P20
T1625 5773-5774 . denotes .
T1626 5774-5871 sentence denotes Of more than 100 homozygotes we have produced, none have survived more than 25 days after birth.
T1627 5775-5777 IN denotes Of
T1628 5832-5840 VBN denotes survived
T1629 5778-5782 JJR denotes more
T1630 5788-5791 CD denotes 100
T1631 5783-5787 IN denotes than
T1632 5792-5803 NNS denotes homozygotes
T1633 5804-5806 PRP denotes we
T1634 5812-5820 VBN denotes produced
T1635 5807-5811 VBP denotes have
T1636 5820-5822 , denotes ,
T1637 5822-5826 NN denotes none
T1638 5827-5831 VBP denotes have
T1639 5841-5845 JJR denotes more
T1640 5851-5853 CD denotes 25
T1641 5846-5850 IN denotes than
T1642 5854-5858 NNS denotes days
T1643 5859-5864 IN denotes after
T1644 5865-5870 NN denotes birth
T1645 5870-5871 . denotes .
T1646 5871-5993 sentence denotes Meanwhile, heterozygous mutants looked normal, were fertile, and survived for more than one year without obvious defects.
T1647 5872-5881 RB denotes Meanwhile
T1648 5904-5910 VBD denotes looked
T1649 5881-5883 , denotes ,
T1650 5883-5895 JJ denotes heterozygous
T1651 5896-5903 NNS denotes mutants
T1652 5911-5917 JJ denotes normal
T1653 5917-5919 , denotes ,
T1654 5919-5923 VBD denotes were
T1655 5924-5931 JJ denotes fertile
T1656 5931-5933 , denotes ,
T1657 5933-5936 CC denotes and
T1658 5937-5945 VBD denotes survived
T1659 5946-5949 IN denotes for
T1660 5950-5954 JJR denotes more
T1661 5960-5963 CD denotes one
T1662 5955-5959 IN denotes than
T1663 5964-5968 NN denotes year
T1664 5969-5976 IN denotes without
T1665 5977-5984 JJ denotes obvious
T1666 5985-5992 NNS denotes defects
T1667 5992-5993 . denotes .
T1960 5995-6009 NN denotes Histopathology
T1961 6010-6012 IN denotes of
T1962 6013-6019 NN denotes ADAM22
T1963 6020-6029 JJ denotes deficient
T1964 6019-6020 HYPH denotes -
T1965 6030-6034 NNS denotes mice
T1966 6034-6157 sentence denotes We have reported the predominant expression of ADAM22 mRNA in the human and mouse brain by Northern blot analysis [13,14].
T1967 6035-6037 PRP denotes We
T1968 6043-6051 VBN denotes reported
T1969 6038-6042 VBP denotes have
T1970 6052-6055 DT denotes the
T1971 6068-6078 NN denotes expression
T1972 6056-6067 JJ denotes predominant
T1973 6079-6081 IN denotes of
T1974 6082-6088 NN denotes ADAM22
T1975 6089-6093 NN denotes mRNA
T1976 6094-6096 IN denotes in
T1977 6097-6100 DT denotes the
T1978 6117-6122 NN denotes brain
T1979 6101-6106 JJ denotes human
T1980 6111-6116 NN denotes mouse
T1981 6107-6110 CC denotes and
T1982 6123-6125 IN denotes by
T1983 6126-6134 NNP denotes Northern
T1984 6135-6139 NN denotes blot
T1985 6140-6148 NN denotes analysis
T1986 6149-6150 -LRB- denotes [
T1987 6153-6155 CD denotes 14
T1988 6150-6152 CD denotes 13
T1989 6152-6153 , denotes ,
T1990 6155-6156 -RRB- denotes ]
T1991 6156-6157 . denotes .
T1992 6157-6316 sentence denotes To determine the distribution of ADAM22 transcript in the adult mouse CNS precisely, in situ hybridisation analysis was performed using 35S-labeled RNA probe.
T1993 6158-6160 TO denotes To
T1994 6161-6170 VB denotes determine
T1995 6278-6287 VBN denotes performed
T1996 6171-6174 DT denotes the
T1997 6175-6187 NN denotes distribution
T1998 6188-6190 IN denotes of
T1999 6191-6197 NN denotes ADAM22
T2000 6198-6208 NN denotes transcript
T2001 6209-6211 IN denotes in
T2002 6212-6215 DT denotes the
T2003 6228-6231 NN denotes CNS
T2004 6216-6221 JJ denotes adult
T2005 6222-6227 NN denotes mouse
T2006 6232-6241 RB denotes precisely
T2007 6241-6243 , denotes ,
T2008 6243-6245 FW denotes in
T2009 6246-6250 FW denotes situ
T2010 6265-6273 NN denotes analysis
T2011 6251-6264 NN denotes hybridisation
T2012 6274-6277 VBD denotes was
T2013 6288-6293 VBG denotes using
T2014 6294-6297 CD denotes 35S
T2015 6298-6305 VBN denotes labeled
T2016 6297-6298 HYPH denotes -
T2017 6310-6315 NN denotes probe
T2018 6306-6309 NN denotes RNA
T2019 6315-6316 . denotes .
T2020 6316-6394 sentence denotes As shown in Fig. 3, ADAM22 mRNA was expressed throughout the adult mouse CNS.
T2021 6317-6319 IN denotes As
T2022 6320-6325 VBN denotes shown
T2023 6353-6362 VBN denotes expressed
T2024 6326-6328 IN denotes in
T2025 6329-6333 NN denotes Fig.
T2026 6334-6335 CD denotes 3
T2027 6335-6337 , denotes ,
T2028 6337-6343 NN denotes ADAM22
T2029 6344-6348 NN denotes mRNA
T2030 6349-6352 VBD denotes was
T2031 6363-6373 IN denotes throughout
T2032 6374-6377 DT denotes the
T2033 6390-6393 NN denotes CNS
T2034 6378-6383 JJ denotes adult
T2035 6384-6389 NN denotes mouse
T2036 6393-6394 . denotes .
T2037 6394-6480 sentence denotes Strong signal was detected in the cerebellar granule cells and hippocampal formation.
T2038 6395-6401 JJ denotes Strong
T2039 6402-6408 NN denotes signal
T2040 6413-6421 VBN denotes detected
T2041 6409-6412 VBD denotes was
T2042 6422-6424 IN denotes in
T2043 6425-6428 DT denotes the
T2044 6448-6453 NNS denotes cells
T2045 6429-6439 JJ denotes cerebellar
T2046 6440-6447 NN denotes granule
T2047 6454-6457 CC denotes and
T2048 6458-6469 JJ denotes hippocampal
T2049 6470-6479 NN denotes formation
T2050 6479-6480 . denotes .
T2051 6480-6556 sentence denotes In the spinal cord, hybridisation signal was restricted to the grey matter.
T2052 6481-6483 IN denotes In
T2053 6526-6536 VBN denotes restricted
T2054 6484-6487 DT denotes the
T2055 6495-6499 NN denotes cord
T2056 6488-6494 JJ denotes spinal
T2057 6499-6501 , denotes ,
T2058 6501-6514 NN denotes hybridisation
T2059 6515-6521 NN denotes signal
T2060 6522-6525 VBD denotes was
T2061 6537-6539 IN denotes to
T2062 6540-6543 DT denotes the
T2063 6549-6555 NN denotes matter
T2064 6544-6548 JJ denotes grey
T2065 6555-6556 . denotes .
T2066 6556-6695 sentence denotes The distribution of ADAM22 transcripts in the CNS was quite similar with that of ADAM11, whose neuronal expression has been reported [19].
T2067 6557-6560 DT denotes The
T2068 6561-6573 NN denotes distribution
T2069 6607-6610 VBD denotes was
T2070 6574-6576 IN denotes of
T2071 6577-6583 NN denotes ADAM22
T2072 6584-6595 NNS denotes transcripts
T2073 6596-6598 IN denotes in
T2074 6599-6602 DT denotes the
T2075 6603-6606 NN denotes CNS
T2076 6611-6616 RB denotes quite
T2077 6617-6624 JJ denotes similar
T2078 6625-6629 IN denotes with
T2079 6630-6634 DT denotes that
T2080 6635-6637 IN denotes of
T2081 6638-6644 NN denotes ADAM11
T2082 6644-6646 , denotes ,
T2083 6646-6651 WP$ denotes whose
T2084 6661-6671 NN denotes expression
T2085 6652-6660 JJ denotes neuronal
T2086 6681-6689 VBN denotes reported
T2087 6672-6675 VBZ denotes has
T2088 6676-6680 VBN denotes been
T2089 6690-6691 -LRB- denotes [
T2090 6691-6693 CD denotes 19
T2091 6693-6694 -RRB- denotes ]
T2092 6694-6695 . denotes .
T2093 6695-6779 sentence denotes These results suggest that the ADAM22 mRNA expression is neuronal in the mouse CNS.
T2094 6696-6701 DT denotes These
T2095 6702-6709 NNS denotes results
T2096 6710-6717 VBP denotes suggest
T2097 6718-6722 IN denotes that
T2098 6750-6752 VBZ denotes is
T2099 6723-6726 DT denotes the
T2100 6739-6749 NN denotes expression
T2101 6727-6733 NN denotes ADAM22
T2102 6734-6738 NN denotes mRNA
T2103 6753-6761 JJ denotes neuronal
T2104 6762-6764 IN denotes in
T2105 6765-6768 DT denotes the
T2106 6775-6778 NN denotes CNS
T2107 6769-6774 NN denotes mouse
T2108 6778-6779 . denotes .
T2109 6779-6912 sentence denotes Based on the mRNA distribution pattern, we performed immunohistopathological analysis of the cerebellum and hippocampus extensively.
T2110 6780-6785 VBN denotes Based
T2111 6823-6832 VBD denotes performed
T2112 6786-6788 IN denotes on
T2113 6789-6792 DT denotes the
T2114 6811-6818 NN denotes pattern
T2115 6793-6797 NN denotes mRNA
T2116 6798-6810 NN denotes distribution
T2117 6818-6820 , denotes ,
T2118 6820-6822 PRP denotes we
T2119 6833-6856 JJ denotes immunohistopathological
T2120 6857-6865 NN denotes analysis
T2121 6866-6868 IN denotes of
T2122 6869-6872 DT denotes the
T2123 6873-6883 NN denotes cerebellum
T2124 6884-6887 CC denotes and
T2125 6888-6899 NN denotes hippocampus
T2126 6900-6911 RB denotes extensively
T2127 6911-6912 . denotes .
T2128 6912-7135 sentence denotes Despite the high level of expression of ADAM22 mRNAs in the cerebellar granule cells, granule cell layer was normally formed and Purkinje cell morphology (calbindin-staining) of the mutant mouse looked intact (Figs. 4A–D).
T2129 6913-6920 IN denotes Despite
T2130 7031-7037 VBN denotes formed
T2131 6921-6924 DT denotes the
T2132 6930-6935 NN denotes level
T2133 6925-6929 JJ denotes high
T2134 6936-6938 IN denotes of
T2135 6939-6949 NN denotes expression
T2136 6950-6952 IN denotes of
T2137 6953-6959 NN denotes ADAM22
T2138 6960-6965 NNS denotes mRNAs
T2139 6966-6968 IN denotes in
T2140 6969-6972 DT denotes the
T2141 6992-6997 NNS denotes cells
T2142 6973-6983 JJ denotes cerebellar
T2143 6984-6991 NN denotes granule
T2144 6997-6999 , denotes ,
T2145 6999-7006 NN denotes granule
T2146 7012-7017 NN denotes layer
T2147 7007-7011 NN denotes cell
T2148 7018-7021 VBD denotes was
T2149 7022-7030 RB denotes normally
T2150 7038-7041 CC denotes and
T2151 7042-7050 NNP denotes Purkinje
T2152 7051-7055 NN denotes cell
T2153 7056-7066 NN denotes morphology
T2154 7108-7114 VBD denotes looked
T2155 7067-7068 -LRB- denotes (
T2156 7078-7086 VBG denotes staining
T2157 7068-7077 NN denotes calbindin
T2158 7077-7078 HYPH denotes -
T2159 7086-7087 -RRB- denotes )
T2160 7088-7090 IN denotes of
T2161 7091-7094 DT denotes the
T2162 7102-7107 NN denotes mouse
T2163 7095-7101 NN denotes mutant
T2164 7115-7121 JJ denotes intact
T2165 7122-7123 -LRB- denotes (
T2166 7129-7131 NN denotes 4A
T2167 7123-7128 NNS denotes Figs.
T2168 7131-7132 SYM denotes –
T2169 7132-7133 NN denotes D
T2170 7133-7134 -RRB- denotes )
T2171 7134-7135 . denotes .
T2172 7135-7196 sentence denotes Hippocampal formation was also normally formed (Figs. 4I–J).
T2173 7136-7147 JJ denotes Hippocampal
T2174 7148-7157 NN denotes formation
T2175 7176-7182 VBN denotes formed
T2176 7158-7161 VBD denotes was
T2177 7162-7166 RB denotes also
T2178 7167-7175 RB denotes normally
T2179 7183-7184 -LRB- denotes (
T2180 7190-7192 NN denotes 4I
T2181 7184-7189 NNS denotes Figs.
T2182 7192-7193 SYM denotes –
T2183 7193-7194 NN denotes J
T2184 7194-7195 -RRB- denotes )
T2185 7195-7196 . denotes .
T2186 7196-7285 sentence denotes These results suggest that neuronal cell migration was not impaired in the mutant mouse.
T2187 7197-7202 DT denotes These
T2188 7203-7210 NNS denotes results
T2189 7211-7218 VBP denotes suggest
T2190 7219-7223 IN denotes that
T2191 7256-7264 VBN denotes impaired
T2192 7224-7232 JJ denotes neuronal
T2193 7233-7237 NN denotes cell
T2194 7238-7247 NN denotes migration
T2195 7248-7251 VBD denotes was
T2196 7252-7255 RB denotes not
T2197 7265-7267 IN denotes in
T2198 7268-7271 DT denotes the
T2199 7279-7284 NN denotes mouse
T2200 7272-7278 NN denotes mutant
T2201 7284-7285 . denotes .
T2202 7285-7489 sentence denotes Myelin-formation detected by MBP (myelin basic protein) staining of the mutant in the cerebellum (Fig. 4H) and spinal cord (Fig. 4L) was also indistinguishable with the wild-type littermate (Figs. 4G,K).
T2203 7286-7292 NN denotes Myelin
T2204 7293-7302 NN denotes formation
T2205 7292-7293 HYPH denotes -
T2206 7419-7422 VBD denotes was
T2207 7303-7311 VBN denotes detected
T2208 7312-7314 IN denotes by
T2209 7315-7318 NN denotes MBP
T2210 7342-7350 NN denotes staining
T2211 7319-7320 -LRB- denotes (
T2212 7320-7326 NN denotes myelin
T2213 7333-7340 NN denotes protein
T2214 7327-7332 JJ denotes basic
T2215 7340-7341 -RRB- denotes )
T2216 7351-7353 IN denotes of
T2217 7354-7357 DT denotes the
T2218 7358-7364 NN denotes mutant
T2219 7365-7367 IN denotes in
T2220 7368-7371 DT denotes the
T2221 7372-7382 NN denotes cerebellum
T2222 7383-7384 -LRB- denotes (
T2223 7389-7391 NN denotes 4H
T2224 7384-7388 NN denotes Fig.
T2225 7391-7392 -RRB- denotes )
T2226 7393-7396 CC denotes and
T2227 7397-7403 JJ denotes spinal
T2228 7404-7408 NN denotes cord
T2229 7409-7410 -LRB- denotes (
T2230 7415-7417 NN denotes 4L
T2231 7410-7414 NN denotes Fig.
T2232 7417-7418 -RRB- denotes )
T2233 7423-7427 RB denotes also
T2234 7428-7445 JJ denotes indistinguishable
T2235 7446-7450 IN denotes with
T2236 7451-7454 DT denotes the
T2237 7465-7475 NN denotes littermate
T2238 7455-7459 JJ denotes wild
T2239 7460-7464 NN denotes type
T2240 7459-7460 HYPH denotes -
T2241 7476-7477 -LRB- denotes (
T2242 7486-7487 NN denotes K
T2243 7477-7482 NNS denotes Figs.
T2244 7483-7485 NN denotes 4G
T2245 7485-7486 , denotes ,
T2246 7487-7488 -RRB- denotes )
T2247 7488-7489 . denotes .
T2248 7489-7610 sentence denotes In summary, we could not find any signs of abnormalities in the mutant mouse brain by the light microscopic examination.
T2249 7490-7492 IN denotes In
T2250 7515-7519 VB denotes find
T2251 7493-7500 NN denotes summary
T2252 7500-7502 , denotes ,
T2253 7502-7504 PRP denotes we
T2254 7505-7510 MD denotes could
T2255 7511-7514 RB denotes not
T2256 7520-7523 DT denotes any
T2257 7524-7529 NNS denotes signs
T2258 7530-7532 IN denotes of
T2259 7533-7546 NNS denotes abnormalities
T2260 7547-7549 IN denotes in
T2261 7550-7553 DT denotes the
T2262 7567-7572 NN denotes brain
T2263 7554-7560 NN denotes mutant
T2264 7561-7566 NN denotes mouse
T2265 7573-7575 IN denotes by
T2266 7576-7579 DT denotes the
T2267 7598-7609 NN denotes examination
T2268 7580-7585 NN denotes light
T2269 7586-7597 JJ denotes microscopic
T2270 7609-7610 . denotes .
T2271 7610-7742 sentence denotes Next, the spinal cord and peripheral nerves of each genotype were analysed by toluidine blue stain, which reveals myelin formation.
T2272 7611-7615 RB denotes Next
T2273 7677-7685 VBN denotes analysed
T2274 7615-7617 , denotes ,
T2275 7617-7620 DT denotes the
T2276 7628-7632 NN denotes cord
T2277 7621-7627 JJ denotes spinal
T2278 7633-7636 CC denotes and
T2279 7637-7647 JJ denotes peripheral
T2280 7648-7654 NNS denotes nerves
T2281 7655-7657 IN denotes of
T2282 7658-7662 DT denotes each
T2283 7663-7671 NN denotes genotype
T2284 7672-7676 VBD denotes were
T2285 7686-7688 IN denotes by
T2286 7689-7698 NN denotes toluidine
T2287 7704-7709 NN denotes stain
T2288 7699-7703 JJ denotes blue
T2289 7709-7711 , denotes ,
T2290 7711-7716 WDT denotes which
T2291 7717-7724 VBZ denotes reveals
T2292 7725-7731 NN denotes myelin
T2293 7732-7741 NN denotes formation
T2294 7741-7742 . denotes .
T2295 7742-7886 sentence denotes Surprisingly, lack of myelin or thin myelin was observed in the sciatic nerves (Fig. 5B) and trigeminal nerves (Fig. 5D) in homozygous mutants.
T2296 7743-7755 RB denotes Surprisingly
T2297 7791-7799 VBN denotes observed
T2298 7755-7757 , denotes ,
T2299 7757-7761 NN denotes lack
T2300 7762-7764 IN denotes of
T2301 7765-7771 NN denotes myelin
T2302 7772-7774 CC denotes or
T2303 7775-7779 JJ denotes thin
T2304 7780-7786 NN denotes myelin
T2305 7787-7790 VBD denotes was
T2306 7800-7802 IN denotes in
T2307 7803-7806 DT denotes the
T2308 7815-7821 NNS denotes nerves
T2309 7807-7814 JJ denotes sciatic
T2310 7822-7823 -LRB- denotes (
T2311 7828-7830 NN denotes 5B
T2312 7823-7827 NN denotes Fig.
T2313 7830-7831 -RRB- denotes )
T2314 7832-7835 CC denotes and
T2315 7836-7846 JJ denotes trigeminal
T2316 7847-7853 NNS denotes nerves
T2317 7854-7855 -LRB- denotes (
T2318 7860-7862 NN denotes 5D
T2319 7855-7859 NN denotes Fig.
T2320 7862-7863 -RRB- denotes )
T2321 7864-7866 IN denotes in
T2322 7867-7877 JJ denotes homozygous
T2323 7878-7885 NNS denotes mutants
T2324 7885-7886 . denotes .
T2325 7886-8045 sentence denotes Because no lesions were observed in the spinal cord (Fig. 5F), it was suggested that Schwann cell specific myelination defect occurred in the homozygous mice.
T2326 7887-7894 IN denotes Because
T2327 7911-7919 VBN denotes observed
T2328 7895-7897 DT denotes no
T2329 7898-7905 NNS denotes lesions
T2330 7906-7910 VBD denotes were
T2331 7957-7966 VBN denotes suggested
T2332 7920-7922 IN denotes in
T2333 7923-7926 DT denotes the
T2334 7934-7938 NN denotes cord
T2335 7927-7933 JJ denotes spinal
T2336 7939-7940 -LRB- denotes (
T2337 7945-7947 NN denotes 5F
T2338 7940-7944 NN denotes Fig.
T2339 7947-7948 -RRB- denotes )
T2340 7948-7950 , denotes ,
T2341 7950-7952 PRP denotes it
T2342 7953-7956 VBD denotes was
T2343 7967-7971 IN denotes that
T2344 8013-8021 VBD denotes occurred
T2345 7972-7979 NNP denotes Schwann
T2346 7980-7984 NN denotes cell
T2347 7985-7993 JJ denotes specific
T2348 8006-8012 NN denotes defect
T2349 7994-8005 NN denotes myelination
T2350 8022-8024 IN denotes in
T2351 8025-8028 DT denotes the
T2352 8040-8044 NNS denotes mice
T2353 8029-8039 JJ denotes homozygous
T2354 8044-8045 . denotes .
T2355 8045-8186 sentence denotes To analyse the state of myelinating Schwann cells and axons, electron microscopic (EM) analysis of the sciatic nerve was performed (Fig. 6).
T2356 8046-8048 TO denotes To
T2357 8049-8056 VB denotes analyse
T2358 8167-8176 VBN denotes performed
T2359 8057-8060 DT denotes the
T2360 8061-8066 NN denotes state
T2361 8067-8069 IN denotes of
T2362 8070-8081 VBG denotes myelinating
T2363 8090-8095 NNS denotes cells
T2364 8082-8089 NNP denotes Schwann
T2365 8096-8099 CC denotes and
T2366 8100-8105 NNS denotes axons
T2367 8105-8107 , denotes ,
T2368 8107-8115 NN denotes electron
T2369 8116-8127 JJ denotes microscopic
T2370 8133-8141 NN denotes analysis
T2371 8128-8129 -LRB- denotes (
T2372 8129-8131 JJ denotes EM
T2373 8131-8132 -RRB- denotes )
T2374 8142-8144 IN denotes of
T2375 8145-8148 DT denotes the
T2376 8157-8162 NN denotes nerve
T2377 8149-8156 JJ denotes sciatic
T2378 8163-8166 VBD denotes was
T2379 8177-8178 -LRB- denotes (
T2380 8178-8182 NN denotes Fig.
T2381 8183-8184 CD denotes 6
T2382 8184-8185 -RRB- denotes )
T2383 8185-8186 . denotes .
T2384 8186-8261 sentence denotes Schwann cells formed thin or no myelin in the homozygous mutant (Fig. 6B).
T2385 8187-8194 NNP denotes Schwann
T2386 8195-8200 NNS denotes cells
T2387 8201-8207 VBD denotes formed
T2388 8208-8212 JJ denotes thin
T2389 8213-8215 CC denotes or
T2390 8216-8218 DT denotes no
T2391 8219-8225 NN denotes myelin
T2392 8226-8228 IN denotes in
T2393 8229-8232 DT denotes the
T2394 8244-8250 NN denotes mutant
T2395 8233-8243 JJ denotes homozygous
T2396 8251-8252 -LRB- denotes (
T2397 8257-8259 NN denotes 6B
T2398 8252-8256 NN denotes Fig.
T2399 8259-8260 -RRB- denotes )
T2400 8260-8261 . denotes .
T2401 8261-8331 sentence denotes In contrast, heterozygous mice showed complete myelination (Fig. 6A).
T2402 8262-8264 IN denotes In
T2403 8293-8299 VBD denotes showed
T2404 8265-8273 NN denotes contrast
T2405 8273-8275 , denotes ,
T2406 8275-8287 JJ denotes heterozygous
T2407 8288-8292 NNS denotes mice
T2408 8300-8308 JJ denotes complete
T2409 8309-8320 NN denotes myelination
T2410 8321-8322 -LRB- denotes (
T2411 8327-8329 NN denotes 6A
T2412 8322-8326 NN denotes Fig.
T2413 8329-8330 -RRB- denotes )
T2414 8330-8331 . denotes .
T2415 8331-8387 sentence denotes Morphology of the axons looked normal in each genotype.
T2416 8332-8342 NN denotes Morphology
T2417 8356-8362 VBD denotes looked
T2418 8343-8345 IN denotes of
T2419 8346-8349 DT denotes the
T2420 8350-8355 NNS denotes axons
T2421 8363-8369 JJ denotes normal
T2422 8370-8372 IN denotes in
T2423 8373-8377 DT denotes each
T2424 8378-8386 NN denotes genotype
T2425 8386-8387 . denotes .
T2426 8387-8566 sentence denotes Immunohistochemical analysis of the sciatic nerve showed that increased number of nuclei (Fig. 7B) and reduced staining of MBP (Fig. 7D) were remarkable in the homozygous mutant.
T2427 8388-8407 JJ denotes Immunohistochemical
T2428 8408-8416 NN denotes analysis
T2429 8438-8444 VBD denotes showed
T2430 8417-8419 IN denotes of
T2431 8420-8423 DT denotes the
T2432 8432-8437 NN denotes nerve
T2433 8424-8431 JJ denotes sciatic
T2434 8445-8449 IN denotes that
T2435 8525-8529 VBD denotes were
T2436 8450-8459 VBN denotes increased
T2437 8460-8466 NN denotes number
T2438 8467-8469 IN denotes of
T2439 8470-8476 NNS denotes nuclei
T2440 8477-8478 -LRB- denotes (
T2441 8483-8485 NN denotes 7B
T2442 8478-8482 NN denotes Fig.
T2443 8485-8486 -RRB- denotes )
T2444 8487-8490 CC denotes and
T2445 8491-8498 VBN denotes reduced
T2446 8499-8507 NN denotes staining
T2447 8508-8510 IN denotes of
T2448 8511-8514 NN denotes MBP
T2449 8515-8516 -LRB- denotes (
T2450 8521-8523 NN denotes 7D
T2451 8516-8520 NN denotes Fig.
T2452 8523-8524 -RRB- denotes )
T2453 8530-8540 JJ denotes remarkable
T2454 8541-8543 IN denotes in
T2455 8544-8547 DT denotes the
T2456 8559-8565 NN denotes mutant
T2457 8548-8558 JJ denotes homozygous
T2458 8565-8566 . denotes .
T2459 8566-8689 sentence denotes The Schwann cell marker, S100 staining signal was intensely observed in the homozygote as well as wild-type (Figs. 7E, F).
T2460 8567-8570 DT denotes The
T2461 8584-8590 NN denotes marker
T2462 8571-8578 NNP denotes Schwann
T2463 8579-8583 NN denotes cell
T2464 8627-8635 VBN denotes observed
T2465 8590-8592 , denotes ,
T2466 8592-8596 NN denotes S100
T2467 8606-8612 NN denotes signal
T2468 8597-8605 NN denotes staining
T2469 8613-8616 VBD denotes was
T2470 8617-8626 RB denotes intensely
T2471 8636-8638 IN denotes in
T2472 8639-8642 DT denotes the
T2473 8643-8653 NN denotes homozygote
T2474 8654-8656 RB denotes as
T2475 8662-8664 IN denotes as
T2476 8657-8661 RB denotes well
T2477 8665-8669 JJ denotes wild
T2478 8670-8674 NN denotes type
T2479 8669-8670 HYPH denotes -
T2480 8675-8676 -LRB- denotes (
T2481 8686-8687 NN denotes F
T2482 8676-8681 NNS denotes Figs.
T2483 8682-8684 NN denotes 7E
T2484 8684-8686 , denotes ,
T2485 8687-8688 -RRB- denotes )
T2486 8688-8689 . denotes .
T2487 8689-8838 sentence denotes These results suggest that proliferation of the Schwann cells was not impaired but differentiation is severely delayed in the ADAM22-deficient mice.
T2488 8690-8695 DT denotes These
T2489 8696-8703 NNS denotes results
T2490 8704-8711 VBP denotes suggest
T2491 8712-8716 IN denotes that
T2492 8752-8755 VBD denotes was
T2493 8717-8730 NN denotes proliferation
T2494 8731-8733 IN denotes of
T2495 8734-8737 DT denotes the
T2496 8746-8751 NNS denotes cells
T2497 8738-8745 NNP denotes Schwann
T2498 8756-8759 RB denotes not
T2499 8760-8768 JJ denotes impaired
T2500 8769-8772 CC denotes but
T2501 8773-8788 NN denotes differentiation
T2502 8789-8791 VBZ denotes is
T2503 8792-8800 RB denotes severely
T2504 8801-8808 JJ denotes delayed
T2505 8809-8811 IN denotes in
T2506 8812-8815 DT denotes the
T2507 8833-8837 NNS denotes mice
T2508 8816-8822 NN denotes ADAM22
T2509 8823-8832 JJ denotes deficient
T2510 8822-8823 HYPH denotes -
T2511 8837-8838 . denotes .
T2746 8840-8845 JJ denotes Novel
T2747 8864-8872 NNS denotes variants
T2748 8846-8852 NN denotes ADAM22
T2749 8853-8863 NN denotes transcript
T2750 8873-8881 VBN denotes isolated
T2751 8882-8886 IN denotes from
T2752 8887-8890 DT denotes the
T2753 8910-8916 NN denotes system
T2754 8891-8901 JJ denotes peripheral
T2755 8902-8909 JJ denotes nervous
T2756 8916-9051 sentence denotes In the case of human ADAM22, isolation of five splicing variants and existence of two terminating exons have been reported [13,20,21].
T2757 8917-8919 IN denotes In
T2758 9031-9039 VBN denotes reported
T2759 8920-8923 DT denotes the
T2760 8924-8928 NN denotes case
T2761 8929-8931 IN denotes of
T2762 8932-8937 JJ denotes human
T2763 8938-8944 NN denotes ADAM22
T2764 8944-8946 , denotes ,
T2765 8946-8955 NN denotes isolation
T2766 8956-8958 IN denotes of
T2767 8959-8963 CD denotes five
T2768 8973-8981 NNS denotes variants
T2769 8964-8972 NN denotes splicing
T2770 8982-8985 CC denotes and
T2771 8986-8995 NN denotes existence
T2772 8996-8998 IN denotes of
T2773 8999-9002 CD denotes two
T2774 9015-9020 NNS denotes exons
T2775 9003-9014 VBG denotes terminating
T2776 9021-9025 VBP denotes have
T2777 9026-9030 VBN denotes been
T2778 9040-9041 -LRB- denotes [
T2779 9047-9049 CD denotes 21
T2780 9041-9043 CD denotes 13
T2781 9043-9044 , denotes ,
T2782 9044-9046 CD denotes 20
T2783 9046-9047 , denotes ,
T2784 9049-9050 -RRB- denotes ]
T2785 9050-9051 . denotes .
T2786 9051-9148 sentence denotes However, the latter terminating exon (exon 31) of the mouse species has not been identified yet.
T2787 9052-9059 RB denotes However
T2788 9133-9143 VBN denotes identified
T2789 9059-9061 , denotes ,
T2790 9061-9064 DT denotes the
T2791 9084-9088 NN denotes exon
T2792 9065-9071 JJ denotes latter
T2793 9072-9083 VBG denotes terminating
T2794 9089-9090 -LRB- denotes (
T2795 9090-9094 NN denotes exon
T2796 9095-9097 CD denotes 31
T2797 9097-9098 -RRB- denotes )
T2798 9099-9101 IN denotes of
T2799 9102-9105 DT denotes the
T2800 9112-9119 NNS denotes species
T2801 9106-9111 NN denotes mouse
T2802 9120-9123 VBZ denotes has
T2803 9124-9127 RB denotes not
T2804 9128-9132 VBN denotes been
T2805 9144-9147 RB denotes yet
T2806 9147-9148 . denotes .
T2807 9148-9265 sentence denotes To isolate the long form of mouse ADAM22 transcripts, at first, we sought the mouse genome by BLAST homology search.
T2808 9149-9151 TO denotes To
T2809 9152-9159 VB denotes isolate
T2810 9216-9222 VBD denotes sought
T2811 9160-9163 DT denotes the
T2812 9169-9173 NN denotes form
T2813 9164-9168 JJ denotes long
T2814 9174-9176 IN denotes of
T2815 9177-9182 NN denotes mouse
T2816 9183-9189 NN denotes ADAM22
T2817 9190-9201 NNS denotes transcripts
T2818 9201-9203 , denotes ,
T2819 9203-9205 IN denotes at
T2820 9206-9211 RB denotes first
T2821 9211-9213 , denotes ,
T2822 9213-9215 PRP denotes we
T2823 9223-9226 DT denotes the
T2824 9233-9239 NN denotes genome
T2825 9227-9232 NN denotes mouse
T2826 9240-9242 IN denotes by
T2827 9243-9248 NNP denotes BLAST
T2828 9249-9257 NN denotes homology
T2829 9258-9264 NN denotes search
T2830 9264-9265 . denotes .
T2831 9265-9367 sentence denotes Search results showed that the mouse genome contig NT_039297 contained most of the mouse Adam22 gene.
T2832 9266-9272 NN denotes Search
T2833 9273-9280 NNS denotes results
T2834 9281-9287 VBD denotes showed
T2835 9288-9292 IN denotes that
T2836 9327-9336 VBD denotes contained
T2837 9293-9296 DT denotes the
T2838 9303-9309 NN denotes genome
T2839 9297-9302 NN denotes mouse
T2840 9310-9316 NN denotes contig
T2841 9317-9326 NN denotes NT_039297
T2842 9337-9341 JJS denotes most
T2843 9342-9344 IN denotes of
T2844 9345-9348 DT denotes the
T2845 9362-9366 NN denotes gene
T2846 9349-9354 NN denotes mouse
T2847 9355-9361 NN denotes Adam22
T2848 9366-9367 . denotes .
T2849 9367-9511 sentence denotes In this contig sequence, we found putative exons 30 and 31, those sequences were quite similar to human ADAM22 isoform 1 (GenBank # NM_021723).
T2850 9368-9370 IN denotes In
T2851 9396-9401 VBD denotes found
T2852 9371-9375 DT denotes this
T2853 9383-9391 NN denotes sequence
T2854 9376-9382 NN denotes contig
T2855 9391-9393 , denotes ,
T2856 9393-9395 PRP denotes we
T2857 9444-9448 VBD denotes were
T2858 9402-9410 JJ denotes putative
T2859 9417-9419 CD denotes 30
T2860 9411-9416 NNS denotes exons
T2861 9420-9423 CC denotes and
T2862 9424-9426 CD denotes 31
T2863 9426-9428 , denotes ,
T2864 9428-9433 DT denotes those
T2865 9434-9443 NNS denotes sequences
T2866 9449-9454 RB denotes quite
T2867 9455-9462 JJ denotes similar
T2868 9463-9465 IN denotes to
T2869 9466-9471 JJ denotes human
T2870 9479-9486 NN denotes isoform
T2871 9472-9478 NN denotes ADAM22
T2872 9487-9488 CD denotes 1
T2873 9489-9490 -LRB- denotes (
T2874 9500-9509 NN denotes NM_021723
T2875 9490-9497 NNP denotes GenBank
T2876 9498-9499 SYM denotes #
T2877 9509-9510 -RRB- denotes )
T2878 9510-9511 . denotes .
T2879 9511-9656 sentence denotes To confirm existence of transcripts, we designed primers on the putative exon 31 and performed RT-PCR using mouse cerebellum mRNA as a template.
T2880 9512-9514 TO denotes To
T2881 9515-9522 VB denotes confirm
T2882 9552-9560 VBD denotes designed
T2883 9523-9532 NN denotes existence
T2884 9533-9535 IN denotes of
T2885 9536-9547 NNS denotes transcripts
T2886 9547-9549 , denotes ,
T2887 9549-9551 PRP denotes we
T2888 9561-9568 NNS denotes primers
T2889 9569-9571 IN denotes on
T2890 9572-9575 DT denotes the
T2891 9585-9589 NN denotes exon
T2892 9576-9584 JJ denotes putative
T2893 9590-9592 CD denotes 31
T2894 9593-9596 CC denotes and
T2895 9597-9606 VBN denotes performed
T2896 9607-9609 NN denotes RT
T2897 9610-9613 NN denotes PCR
T2898 9609-9610 HYPH denotes -
T2899 9614-9619 VBG denotes using
T2900 9620-9625 NN denotes mouse
T2901 9637-9641 NN denotes mRNA
T2902 9626-9636 NN denotes cerebellum
T2903 9642-9644 IN denotes as
T2904 9645-9646 DT denotes a
T2905 9647-9655 NN denotes template
T2906 9655-9656 . denotes .
T2907 9656-9872 sentence denotes TA-cloning of the amplified fragments and sequencing analysis showed that 5 of 8 clones were type 1 isoform (G01), 2 clones were type 3 isoform (G03) and 1 clone (G08) contained a novel exon between exons 29 and 30.
T2908 9657-9659 NN denotes TA
T2909 9660-9667 NN denotes cloning
T2910 9659-9660 HYPH denotes -
T2911 9719-9725 VBD denotes showed
T2912 9668-9670 IN denotes of
T2913 9671-9674 DT denotes the
T2914 9685-9694 NNS denotes fragments
T2915 9675-9684 VBN denotes amplified
T2916 9695-9698 CC denotes and
T2917 9699-9709 NN denotes sequencing
T2918 9710-9718 NN denotes analysis
T2919 9726-9730 IN denotes that
T2920 9745-9749 VBD denotes were
T2921 9731-9732 CD denotes 5
T2922 9736-9737 CD denotes 8
T2923 9733-9735 IN denotes of
T2924 9738-9744 NNS denotes clones
T2925 9750-9754 NN denotes type
T2926 9757-9764 NN denotes isoform
T2927 9755-9756 CD denotes 1
T2928 9765-9766 -LRB- denotes (
T2929 9766-9769 NN denotes G01
T2930 9769-9770 -RRB- denotes )
T2931 9770-9772 , denotes ,
T2932 9772-9773 CD denotes 2
T2933 9774-9780 NNS denotes clones
T2934 9781-9785 VBD denotes were
T2935 9786-9790 NN denotes type
T2936 9793-9800 NN denotes isoform
T2937 9791-9792 CD denotes 3
T2938 9801-9802 -LRB- denotes (
T2939 9802-9805 NN denotes G03
T2940 9805-9806 -RRB- denotes )
T2941 9807-9810 CC denotes and
T2942 9811-9812 CD denotes 1
T2943 9813-9818 NN denotes clone
T2944 9825-9834 VBD denotes contained
T2945 9819-9820 -LRB- denotes (
T2946 9820-9823 NN denotes G08
T2947 9823-9824 -RRB- denotes )
T2948 9835-9836 DT denotes a
T2949 9843-9847 NN denotes exon
T2950 9837-9842 JJ denotes novel
T2951 9848-9855 IN denotes between
T2952 9856-9861 NNS denotes exons
T2953 9862-9864 CD denotes 29
T2954 9865-9868 CC denotes and
T2955 9869-9871 CD denotes 30
T2956 9871-9872 . denotes .
T2957 9872-10062 sentence denotes Next, we performed RT-PCR analysis of mRNAs purified from several adult mouse tissues, such as the spinal cord, dorsal root ganglion (DRG), sciatic nerve and cultured primary Schwann cells.
T2958 9873-9877 RB denotes Next
T2959 9882-9891 VBD denotes performed
T2960 9877-9879 , denotes ,
T2961 9879-9881 PRP denotes we
T2962 9892-9894 NN denotes RT
T2963 9895-9898 NN denotes PCR
T2964 9894-9895 HYPH denotes -
T2965 9899-9907 NN denotes analysis
T2966 9908-9910 IN denotes of
T2967 9911-9916 NNS denotes mRNAs
T2968 9917-9925 VBN denotes purified
T2969 9926-9930 IN denotes from
T2970 9931-9938 JJ denotes several
T2971 9951-9958 NNS denotes tissues
T2972 9939-9944 JJ denotes adult
T2973 9945-9950 NN denotes mouse
T2974 9958-9960 , denotes ,
T2975 9960-9964 JJ denotes such
T2976 9965-9967 IN denotes as
T2977 9968-9971 DT denotes the
T2978 9979-9983 NN denotes cord
T2979 9972-9978 JJ denotes spinal
T2980 9983-9985 , denotes ,
T2981 9985-9991 JJ denotes dorsal
T2982 9992-9996 NN denotes root
T2983 9997-10005 NN denotes ganglion
T2984 10006-10007 -LRB- denotes (
T2985 10007-10010 NN denotes DRG
T2986 10010-10011 -RRB- denotes )
T2987 10011-10013 , denotes ,
T2988 10013-10020 JJ denotes sciatic
T2989 10021-10026 NN denotes nerve
T2990 10027-10030 CC denotes and
T2991 10031-10039 VBN denotes cultured
T2992 10056-10061 NNS denotes cells
T2993 10040-10047 JJ denotes primary
T2994 10048-10055 NNP denotes Schwann
T2995 10061-10062 . denotes .
T2996 10062-10200 sentence denotes Interestingly, multiple DNA fragments were amplified from each tissue (Fig. 8), and length of major fragments in each lane was different.
T2997 10063-10076 RB denotes Interestingly
T2998 10106-10115 VBN denotes amplified
T2999 10076-10078 , denotes ,
T3000 10078-10086 JJ denotes multiple
T3001 10091-10100 NNS denotes fragments
T3002 10087-10090 NN denotes DNA
T3003 10101-10105 VBD denotes were
T3004 10116-10120 IN denotes from
T3005 10121-10125 DT denotes each
T3006 10126-10132 NN denotes tissue
T3007 10133-10134 -LRB- denotes (
T3008 10134-10138 NN denotes Fig.
T3009 10139-10140 CD denotes 8
T3010 10140-10141 -RRB- denotes )
T3011 10141-10143 , denotes ,
T3012 10143-10146 CC denotes and
T3013 10147-10153 NN denotes length
T3014 10186-10189 VBD denotes was
T3015 10154-10156 IN denotes of
T3016 10157-10162 JJ denotes major
T3017 10163-10172 NNS denotes fragments
T3018 10173-10175 IN denotes in
T3019 10176-10180 DT denotes each
T3020 10181-10185 NN denotes lane
T3021 10190-10199 JJ denotes different
T3022 10199-10200 . denotes .
T3023 10200-10401 sentence denotes For example, length of major amplified PCR fragments from the sciatic nerve (Fig. 8A, lane 5) and cultured Schwann cells (lane 6) were almost identical, and were shorter than those from other tissues.
T3024 10201-10204 IN denotes For
T3025 10331-10335 VBD denotes were
T3026 10205-10212 NN denotes example
T3027 10212-10214 , denotes ,
T3028 10214-10220 NN denotes length
T3029 10221-10223 IN denotes of
T3030 10224-10229 JJ denotes major
T3031 10244-10253 NNS denotes fragments
T3032 10230-10239 VBN denotes amplified
T3033 10240-10243 NN denotes PCR
T3034 10254-10258 IN denotes from
T3035 10259-10262 DT denotes the
T3036 10271-10276 NN denotes nerve
T3037 10263-10270 JJ denotes sciatic
T3038 10277-10278 -LRB- denotes (
T3039 10287-10291 NN denotes lane
T3040 10278-10282 NN denotes Fig.
T3041 10283-10285 NN denotes 8A
T3042 10285-10287 , denotes ,
T3043 10292-10293 CD denotes 5
T3044 10293-10294 -RRB- denotes )
T3045 10295-10298 CC denotes and
T3046 10299-10307 VBN denotes cultured
T3047 10316-10321 NNS denotes cells
T3048 10308-10315 NNP denotes Schwann
T3049 10322-10323 -LRB- denotes (
T3050 10323-10327 NN denotes lane
T3051 10328-10329 CD denotes 6
T3052 10329-10330 -RRB- denotes )
T3053 10336-10342 RB denotes almost
T3054 10343-10352 JJ denotes identical
T3055 10352-10354 , denotes ,
T3056 10354-10357 CC denotes and
T3057 10358-10362 VBD denotes were
T3058 10363-10370 JJR denotes shorter
T3059 10371-10375 IN denotes than
T3060 10376-10381 DT denotes those
T3061 10382-10386 IN denotes from
T3062 10387-10392 JJ denotes other
T3063 10393-10400 NNS denotes tissues
T3064 10400-10401 . denotes .
T3065 10401-10477 sentence denotes In contrast, major fragments from the DRG (lane 4) were longer than others.
T3066 10402-10404 IN denotes In
T3067 10453-10457 VBD denotes were
T3068 10405-10413 NN denotes contrast
T3069 10413-10415 , denotes ,
T3070 10415-10420 JJ denotes major
T3071 10421-10430 NNS denotes fragments
T3072 10431-10435 IN denotes from
T3073 10436-10439 DT denotes the
T3074 10440-10443 NN denotes DRG
T3075 10444-10445 -LRB- denotes (
T3076 10445-10449 NN denotes lane
T3077 10450-10451 CD denotes 4
T3078 10451-10452 -RRB- denotes )
T3079 10458-10464 JJR denotes longer
T3080 10465-10469 IN denotes than
T3081 10470-10476 NNS denotes others
T3082 10476-10477 . denotes .
T3083 10477-10585 sentence denotes We performed TA-cloning and sequencing to analyse exon organization of the amplified transcripts (Fig. 8C).
T3084 10478-10480 PRP denotes We
T3085 10481-10490 VBD denotes performed
T3086 10491-10493 NN denotes TA
T3087 10493-10494 HYPH denotes -
T3088 10494-10501 VBG denotes cloning
T3089 10502-10505 CC denotes and
T3090 10506-10516 NN denotes sequencing
T3091 10517-10519 TO denotes to
T3092 10520-10527 VB denotes analyse
T3093 10528-10532 NN denotes exon
T3094 10533-10545 NN denotes organization
T3095 10546-10548 IN denotes of
T3096 10549-10552 DT denotes the
T3097 10563-10574 NNS denotes transcripts
T3098 10553-10562 VBN denotes amplified
T3099 10575-10576 -LRB- denotes (
T3100 10581-10583 NN denotes 8C
T3101 10576-10580 NN denotes Fig.
T3102 10583-10584 -RRB- denotes )
T3103 10584-10585 . denotes .
T3104 10585-10685 sentence denotes Forty seven clones were isolated in total and were classified in 16 independent clones (G01 – G23).
T3105 10586-10591 CD denotes Forty
T3106 10592-10597 CD denotes seven
T3107 10598-10604 NNS denotes clones
T3108 10610-10618 VBN denotes isolated
T3109 10605-10609 VBD denotes were
T3110 10619-10621 IN denotes in
T3111 10622-10627 JJ denotes total
T3112 10628-10631 CC denotes and
T3113 10632-10636 VBD denotes were
T3114 10637-10647 VBN denotes classified
T3115 10648-10650 IN denotes in
T3116 10651-10653 CD denotes 16
T3117 10666-10672 NNS denotes clones
T3118 10654-10665 JJ denotes independent
T3119 10673-10674 -LRB- denotes (
T3120 10674-10677 NN denotes G01
T3121 10678-10679 SYM denotes –
T3122 10680-10683 NN denotes G23
T3123 10683-10684 -RRB- denotes )
T3124 10684-10685 . denotes .
T3125 10685-10815 sentence denotes Comparison with the genomic sequence revealed the existence of 8 novel exons (exons 27S, 27L, 29.1, 29.3, 29.5, 29.7, 30 and 31).
T3126 10686-10696 NN denotes Comparison
T3127 10723-10731 VBD denotes revealed
T3128 10697-10701 IN denotes with
T3129 10702-10705 DT denotes the
T3130 10714-10722 NN denotes sequence
T3131 10706-10713 JJ denotes genomic
T3132 10732-10735 DT denotes the
T3133 10736-10745 NN denotes existence
T3134 10746-10748 IN denotes of
T3135 10749-10750 CD denotes 8
T3136 10757-10762 NNS denotes exons
T3137 10751-10756 JJ denotes novel
T3138 10763-10764 -LRB- denotes (
T3139 10764-10769 NNS denotes exons
T3140 10770-10773 NN denotes 27S
T3141 10773-10775 , denotes ,
T3142 10775-10778 NN denotes 27L
T3143 10778-10780 , denotes ,
T3144 10780-10784 CD denotes 29.1
T3145 10784-10786 , denotes ,
T3146 10786-10790 CD denotes 29.3
T3147 10790-10792 , denotes ,
T3148 10792-10796 CD denotes 29.5
T3149 10796-10798 , denotes ,
T3150 10798-10802 CD denotes 29.7
T3151 10802-10804 , denotes ,
T3152 10804-10806 CD denotes 30
T3153 10807-10810 CC denotes and
T3154 10811-10813 CD denotes 31
T3155 10813-10814 -RRB- denotes )
T3156 10814-10815 . denotes .
T3157 10815-10896 sentence denotes These novel exons were flanked by well-defined introns that obey the GT-AG rule.
T3158 10816-10821 DT denotes These
T3159 10828-10833 NNS denotes exons
T3160 10822-10827 JJ denotes novel
T3161 10839-10846 VBN denotes flanked
T3162 10834-10838 VBD denotes were
T3163 10847-10849 IN denotes by
T3164 10850-10854 RB denotes well
T3165 10855-10862 VBN denotes defined
T3166 10854-10855 HYPH denotes -
T3167 10863-10870 NNS denotes introns
T3168 10871-10875 WDT denotes that
T3169 10876-10880 VBP denotes obey
T3170 10881-10884 DT denotes the
T3171 10891-10895 NN denotes rule
T3172 10885-10887 NN denotes GT
T3173 10888-10890 NN denotes AG
T3174 10887-10888 HYPH denotes -
T3175 10895-10896 . denotes .
T3176 10896-10950 sentence denotes Exon organization of each clone was shown in Fig. 8C.
T3177 10897-10901 NN denotes Exon
T3178 10902-10914 NN denotes organization
T3179 10933-10938 VBN denotes shown
T3180 10915-10917 IN denotes of
T3181 10918-10922 DT denotes each
T3182 10923-10928 NN denotes clone
T3183 10929-10932 VBD denotes was
T3184 10939-10941 IN denotes in
T3185 10942-10946 NN denotes Fig.
T3186 10947-10949 NN denotes 8C
T3187 10949-10950 . denotes .
T3188 10950-11046 sentence denotes Characteristic feature of ADAM22 transcripts is tissue specific insertion or skipping of exons.
T3189 10951-10965 JJ denotes Characteristic
T3190 10966-10973 NN denotes feature
T3191 10996-10998 VBZ denotes is
T3192 10974-10976 IN denotes of
T3193 10977-10983 NN denotes ADAM22
T3194 10984-10995 NNS denotes transcripts
T3195 10999-11005 NN denotes tissue
T3196 11006-11014 JJ denotes specific
T3197 11015-11024 NN denotes insertion
T3198 11025-11027 CC denotes or
T3199 11028-11036 NN denotes skipping
T3200 11037-11039 IN denotes of
T3201 11040-11045 NNS denotes exons
T3202 11045-11046 . denotes .
T3203 11046-11225 sentence denotes Because the number of nucleotides of exons 26, 27, 27L, 29.3, 29.5 and 29.7 is a multiple of 3, insertion or skipping of these exons will not change the downstream reading frame.
T3204 11047-11054 IN denotes Because
T3205 11123-11125 VBZ denotes is
T3206 11055-11058 DT denotes the
T3207 11059-11065 NN denotes number
T3208 11066-11068 IN denotes of
T3209 11069-11080 NNS denotes nucleotides
T3210 11081-11083 IN denotes of
T3211 11084-11089 NNS denotes exons
T3212 11090-11092 CD denotes 26
T3213 11092-11094 , denotes ,
T3214 11094-11096 CD denotes 27
T3215 11096-11098 , denotes ,
T3216 11098-11101 CD denotes 27L
T3217 11101-11103 , denotes ,
T3218 11103-11107 CD denotes 29.3
T3219 11107-11109 , denotes ,
T3220 11109-11113 CD denotes 29.5
T3221 11114-11117 CC denotes and
T3222 11118-11122 CD denotes 29.7
T3223 11189-11195 VB denotes change
T3224 11126-11127 DT denotes a
T3225 11128-11136 NN denotes multiple
T3226 11137-11139 IN denotes of
T3227 11140-11141 CD denotes 3
T3228 11141-11143 , denotes ,
T3229 11143-11152 NN denotes insertion
T3230 11153-11155 CC denotes or
T3231 11156-11164 NN denotes skipping
T3232 11165-11167 IN denotes of
T3233 11168-11173 DT denotes these
T3234 11174-11179 NNS denotes exons
T3235 11180-11184 MD denotes will
T3236 11185-11188 RB denotes not
T3237 11196-11199 DT denotes the
T3238 11219-11224 NN denotes frame
T3239 11200-11210 JJ denotes downstream
T3240 11211-11218 NN denotes reading
T3241 11224-11225 . denotes .
T3242 11225-11295 sentence denotes Known peptide motives were not found in the newly isolated sequences.
T3243 11226-11231 JJ denotes Known
T3244 11240-11247 NNS denotes motives
T3245 11232-11239 NN denotes peptide
T3246 11257-11262 VBN denotes found
T3247 11248-11252 VBD denotes were
T3248 11253-11256 RB denotes not
T3249 11263-11265 IN denotes in
T3250 11266-11269 DT denotes the
T3251 11285-11294 NNS denotes sequences
T3252 11270-11275 RB denotes newly
T3253 11276-11284 VBN denotes isolated
T3254 11294-11295 . denotes .
T3255 11295-11509 sentence denotes This RT-PCR analysis is summarized as follows: ADAM22 transcripts were roughly subdivided in 3 groups, CNS-form (containing exon 27), DRG-form (containing exon 27L) and Schwann-cell-form (skipping exon 26 and 27).
T3256 11296-11300 DT denotes This
T3257 11308-11316 NN denotes analysis
T3258 11301-11303 NN denotes RT
T3259 11304-11307 NN denotes PCR
T3260 11303-11304 HYPH denotes -
T3261 11320-11330 VBN denotes summarized
T3262 11317-11319 VBZ denotes is
T3263 11375-11385 VBN denotes subdivided
T3264 11331-11333 IN denotes as
T3265 11334-11341 VBZ denotes follows
T3266 11341-11343 : denotes :
T3267 11343-11349 NN denotes ADAM22
T3268 11350-11361 NNS denotes transcripts
T3269 11362-11366 VBD denotes were
T3270 11367-11374 RB denotes roughly
T3271 11386-11388 IN denotes in
T3272 11389-11390 CD denotes 3
T3273 11391-11397 NNS denotes groups
T3274 11397-11399 , denotes ,
T3275 11399-11402 NN denotes CNS
T3276 11403-11407 NN denotes form
T3277 11402-11403 HYPH denotes -
T3278 11408-11409 -LRB- denotes (
T3279 11409-11419 VBG denotes containing
T3280 11420-11424 NN denotes exon
T3281 11425-11427 CD denotes 27
T3282 11427-11428 -RRB- denotes )
T3283 11428-11430 , denotes ,
T3284 11430-11433 NN denotes DRG
T3285 11434-11438 NN denotes form
T3286 11433-11434 HYPH denotes -
T3287 11439-11440 -LRB- denotes (
T3288 11440-11450 VBG denotes containing
T3289 11451-11455 NN denotes exon
T3290 11456-11459 NN denotes 27L
T3291 11459-11460 -RRB- denotes )
T3292 11461-11464 CC denotes and
T3293 11465-11472 NNP denotes Schwann
T3294 11473-11477 NN denotes cell
T3295 11472-11473 HYPH denotes -
T3296 11478-11482 NN denotes form
T3297 11477-11478 HYPH denotes -
T3298 11483-11484 -LRB- denotes (
T3299 11484-11492 VBG denotes skipping
T3300 11493-11497 NN denotes exon
T3301 11498-11500 CD denotes 26
T3302 11501-11504 CC denotes and
T3303 11505-11507 CD denotes 27
T3304 11507-11508 -RRB- denotes )
T3305 11508-11509 . denotes .
T3306 11509-11657 sentence denotes From these results, we concluded that ADAM22 is expressed in a cell-type specific manner, and plays an essential role in myelinogenesis in the PNS.
T3307 11510-11514 IN denotes From
T3308 11533-11542 VBD denotes concluded
T3309 11515-11520 DT denotes these
T3310 11521-11528 NNS denotes results
T3311 11528-11530 , denotes ,
T3312 11530-11532 PRP denotes we
T3313 11543-11547 IN denotes that
T3314 11558-11567 VBN denotes expressed
T3315 11548-11554 NN denotes ADAM22
T3316 11555-11557 VBZ denotes is
T3317 11568-11570 IN denotes in
T3318 11571-11572 DT denotes a
T3319 11592-11598 NN denotes manner
T3320 11573-11577 NN denotes cell
T3321 11578-11582 NN denotes type
T3322 11577-11578 HYPH denotes -
T3323 11583-11591 JJ denotes specific
T3324 11598-11600 , denotes ,
T3325 11600-11603 CC denotes and
T3326 11604-11609 VBZ denotes plays
T3327 11610-11612 DT denotes an
T3328 11623-11627 NN denotes role
T3329 11613-11622 JJ denotes essential
T3330 11628-11630 IN denotes in
T3331 11631-11645 NN denotes myelinogenesis
T3332 11646-11648 IN denotes in
T3333 11649-11652 DT denotes the
T3334 11653-11656 NN denotes PNS
T3335 11656-11657 . denotes .
T3705 11670-11672 IN denotes In
T3706 11695-11703 VBD denotes examined
T3707 11673-11676 DT denotes the
T3708 11685-11690 NN denotes study
T3709 11677-11684 JJ denotes present
T3710 11690-11692 , denotes ,
T3711 11692-11694 PRP denotes we
T3712 11704-11707 DT denotes the
T3713 11708-11716 NN denotes function
T3714 11717-11719 IN denotes of
T3715 11720-11726 NN denotes ADAM22
T3716 11727-11729 IN denotes by
T3717 11730-11740 VBG denotes generating
T3718 11741-11745 NNS denotes mice
T3719 11746-11750 WDT denotes that
T3720 11751-11757 VBD denotes lacked
T3721 11758-11761 DT denotes the
T3722 11769-11773 NN denotes gene
T3723 11762-11768 NN denotes Adam22
T3724 11773-11774 . denotes .
T3725 11774-11841 sentence denotes ADAM22-deficient mice exhibited severe ataxia and premature death.
T3726 11775-11781 NN denotes ADAM22
T3727 11782-11791 JJ denotes deficient
T3728 11781-11782 HYPH denotes -
T3729 11792-11796 NNS denotes mice
T3730 11797-11806 VBD denotes exhibited
T3731 11807-11813 JJ denotes severe
T3732 11814-11820 NN denotes ataxia
T3733 11821-11824 CC denotes and
T3734 11825-11834 JJ denotes premature
T3735 11835-11840 NN denotes death
T3736 11840-11841 . denotes .
T3737 11841-11958 sentence denotes In contrast, heterozygous mutants were healthy, fertile and survived more than 1 year without obvious abnormalities.
T3738 11842-11844 IN denotes In
T3739 11876-11880 VBD denotes were
T3740 11845-11853 NN denotes contrast
T3741 11853-11855 , denotes ,
T3742 11855-11867 JJ denotes heterozygous
T3743 11868-11875 NNS denotes mutants
T3744 11881-11888 JJ denotes healthy
T3745 11890-11897 JJ denotes fertile
T3746 11888-11890 , denotes ,
T3747 11898-11901 CC denotes and
T3748 11902-11910 VBD denotes survived
T3749 11911-11915 JJR denotes more
T3750 11921-11922 CD denotes 1
T3751 11916-11920 IN denotes than
T3752 11923-11927 NN denotes year
T3753 11928-11935 IN denotes without
T3754 11936-11943 JJ denotes obvious
T3755 11944-11957 NNS denotes abnormalities
T3756 11957-11958 . denotes .
T3757 11958-12071 sentence denotes The cause of death in the homozygous mutants is not clear; however, it is likely to be due to multiple seizures.
T3758 11959-11962 DT denotes The
T3759 11963-11968 NN denotes cause
T3760 12004-12006 VBZ denotes is
T3761 11969-11971 IN denotes of
T3762 11972-11977 NN denotes death
T3763 11978-11980 IN denotes in
T3764 11981-11984 DT denotes the
T3765 11996-12003 NNS denotes mutants
T3766 11985-11995 JJ denotes homozygous
T3767 12030-12032 VBZ denotes is
T3768 12007-12010 RB denotes not
T3769 12011-12016 JJ denotes clear
T3770 12016-12017 : denotes ;
T3771 12018-12025 RB denotes however
T3772 12025-12027 , denotes ,
T3773 12027-12029 PRP denotes it
T3774 12033-12039 JJ denotes likely
T3775 12040-12042 TO denotes to
T3776 12043-12045 VB denotes be
T3777 12046-12049 IN denotes due
T3778 12050-12052 IN denotes to
T3779 12053-12061 JJ denotes multiple
T3780 12062-12070 NNS denotes seizures
T3781 12070-12071 . denotes .
T3782 12071-12241 sentence denotes This is because convulsive seizures were occasionally observed in homozygotes, after which the suffered mice appeared exhausted and, in most cases, died on the next day.
T3783 12072-12076 DT denotes This
T3784 12077-12079 VBZ denotes is
T3785 12080-12087 IN denotes because
T3786 12126-12134 VBN denotes observed
T3787 12088-12098 JJ denotes convulsive
T3788 12099-12107 NNS denotes seizures
T3789 12108-12112 VBD denotes were
T3790 12113-12125 RB denotes occasionally
T3791 12135-12137 IN denotes in
T3792 12138-12149 NNS denotes homozygotes
T3793 12149-12151 , denotes ,
T3794 12151-12156 IN denotes after
T3795 12181-12189 VBD denotes appeared
T3796 12157-12162 WDT denotes which
T3797 12163-12166 DT denotes the
T3798 12176-12180 NNS denotes mice
T3799 12167-12175 VBN denotes suffered
T3800 12190-12199 JJ denotes exhausted
T3801 12200-12203 CC denotes and
T3802 12203-12205 , denotes ,
T3803 12205-12207 IN denotes in
T3804 12220-12224 VBD denotes died
T3805 12208-12212 JJS denotes most
T3806 12213-12218 NNS denotes cases
T3807 12218-12220 , denotes ,
T3808 12225-12227 IN denotes on
T3809 12228-12231 DT denotes the
T3810 12237-12240 NN denotes day
T3811 12232-12236 JJ denotes next
T3812 12240-12241 . denotes .
T3813 12241-12349 sentence denotes It is known that cortical dysplasia resulting from aberrant brain development causes seizure syndrome [22].
T3814 12242-12244 PRP denotes It
T3815 12248-12253 VBN denotes known
T3816 12245-12247 VBZ denotes is
T3817 12254-12258 IN denotes that
T3818 12320-12326 VBZ denotes causes
T3819 12259-12267 JJ denotes cortical
T3820 12268-12277 NN denotes dysplasia
T3821 12278-12287 VBG denotes resulting
T3822 12288-12292 IN denotes from
T3823 12293-12301 JJ denotes aberrant
T3824 12308-12319 NN denotes development
T3825 12302-12307 NN denotes brain
T3826 12327-12334 NN denotes seizure
T3827 12335-12343 NN denotes syndrome
T3828 12344-12345 -LRB- denotes [
T3829 12345-12347 CD denotes 22
T3830 12347-12348 -RRB- denotes ]
T3831 12348-12349 . denotes .
T3832 12349-12485 sentence denotes For example, mice lacking the neuron-specific activator of cyclin 5, p35, exhibit seizures and a severe neuronal migration defect [23].
T3833 12350-12353 IN denotes For
T3834 12424-12431 VBP denotes exhibit
T3835 12354-12361 NN denotes example
T3836 12361-12363 , denotes ,
T3837 12363-12367 NNS denotes mice
T3838 12368-12375 VBG denotes lacking
T3839 12376-12379 DT denotes the
T3840 12396-12405 NN denotes activator
T3841 12380-12386 NN denotes neuron
T3842 12387-12395 JJ denotes specific
T3843 12386-12387 HYPH denotes -
T3844 12406-12408 IN denotes of
T3845 12409-12415 NN denotes cyclin
T3846 12416-12417 CD denotes 5
T3847 12417-12419 , denotes ,
T3848 12419-12422 NN denotes p35
T3849 12422-12424 , denotes ,
T3850 12432-12440 NNS denotes seizures
T3851 12441-12444 CC denotes and
T3852 12445-12446 DT denotes a
T3853 12473-12479 NN denotes defect
T3854 12447-12453 JJ denotes severe
T3855 12454-12462 JJ denotes neuronal
T3856 12463-12472 NN denotes migration
T3857 12480-12481 -LRB- denotes [
T3858 12481-12483 CD denotes 23
T3859 12483-12484 -RRB- denotes ]
T3860 12484-12485 . denotes .
T3861 12485-12697 sentence denotes Because ADAM22 protein is expressed on the cell surface and is likely to be involved in cell-cell or cell-matrix interactions, we hypothesized that depletion of ADAM22 would generate aberrant neuronal migration.
T3862 12486-12493 IN denotes Because
T3863 12512-12521 VBN denotes expressed
T3864 12494-12500 NN denotes ADAM22
T3865 12501-12508 NN denotes protein
T3866 12509-12511 VBZ denotes is
T3867 12616-12628 VBD denotes hypothesized
T3868 12522-12524 IN denotes on
T3869 12525-12528 DT denotes the
T3870 12534-12541 NN denotes surface
T3871 12529-12533 NN denotes cell
T3872 12542-12545 CC denotes and
T3873 12546-12548 VBZ denotes is
T3874 12549-12555 JJ denotes likely
T3875 12556-12558 TO denotes to
T3876 12562-12570 VBN denotes involved
T3877 12559-12561 VB denotes be
T3878 12571-12573 IN denotes in
T3879 12574-12578 NN denotes cell
T3880 12579-12583 NN denotes cell
T3881 12578-12579 HYPH denotes -
T3882 12599-12611 NNS denotes interactions
T3883 12584-12586 CC denotes or
T3884 12587-12591 NN denotes cell
T3885 12592-12598 NN denotes matrix
T3886 12591-12592 HYPH denotes -
T3887 12611-12613 , denotes ,
T3888 12613-12615 PRP denotes we
T3889 12629-12633 IN denotes that
T3890 12660-12668 VB denotes generate
T3891 12634-12643 NN denotes depletion
T3892 12644-12646 IN denotes of
T3893 12647-12653 NN denotes ADAM22
T3894 12654-12659 MD denotes would
T3895 12669-12677 JJ denotes aberrant
T3896 12687-12696 NN denotes migration
T3897 12678-12686 JJ denotes neuronal
T3898 12696-12697 . denotes .
T3899 12697-12761 sentence denotes Histochemical analysis was performed to verify this hypothesis.
T3900 12698-12711 JJ denotes Histochemical
T3901 12712-12720 NN denotes analysis
T3902 12725-12734 VBN denotes performed
T3903 12721-12724 VBD denotes was
T3904 12735-12737 TO denotes to
T3905 12738-12744 VB denotes verify
T3906 12745-12749 DT denotes this
T3907 12750-12760 NN denotes hypothesis
T3908 12760-12761 . denotes .
T3909 12761-12896 sentence denotes However, no marked abnormalities were observed in the mutant mouse brain, ruling out a critical role for ADAM22 in neuronal migration.
T3910 12762-12769 RB denotes However
T3911 12800-12808 VBN denotes observed
T3912 12769-12771 , denotes ,
T3913 12771-12773 DT denotes no
T3914 12781-12794 NNS denotes abnormalities
T3915 12774-12780 JJ denotes marked
T3916 12795-12799 VBD denotes were
T3917 12809-12811 IN denotes in
T3918 12812-12815 DT denotes the
T3919 12829-12834 NN denotes brain
T3920 12816-12822 NN denotes mutant
T3921 12823-12828 NN denotes mouse
T3922 12834-12836 , denotes ,
T3923 12836-12842 VBG denotes ruling
T3924 12843-12846 RP denotes out
T3925 12847-12848 DT denotes a
T3926 12858-12862 NN denotes role
T3927 12849-12857 JJ denotes critical
T3928 12863-12866 IN denotes for
T3929 12867-12873 NN denotes ADAM22
T3930 12874-12876 IN denotes in
T3931 12877-12885 JJ denotes neuronal
T3932 12886-12895 NN denotes migration
T3933 12895-12896 . denotes .
T3934 12896-13040 sentence denotes Another possible explanation is that the premature death in first 2 weeks of life is caused by the dysfunction of the autonomic nervous system.
T3935 12897-12904 DT denotes Another
T3936 12914-12925 NN denotes explanation
T3937 12905-12913 JJ denotes possible
T3938 12926-12928 VBZ denotes is
T3939 12929-12933 IN denotes that
T3940 12982-12988 VBN denotes caused
T3941 12934-12937 DT denotes the
T3942 12948-12953 NN denotes death
T3943 12938-12947 JJ denotes premature
T3944 12954-12956 IN denotes in
T3945 12957-12962 JJ denotes first
T3946 12965-12970 NNS denotes weeks
T3947 12963-12964 CD denotes 2
T3948 12971-12973 IN denotes of
T3949 12974-12978 NN denotes life
T3950 12979-12981 VBZ denotes is
T3951 12989-12991 IN denotes by
T3952 12992-12995 DT denotes the
T3953 12996-13007 NN denotes dysfunction
T3954 13008-13010 IN denotes of
T3955 13011-13014 DT denotes the
T3956 13033-13039 NN denotes system
T3957 13015-13024 JJ denotes autonomic
T3958 13025-13032 JJ denotes nervous
T3959 13039-13040 . denotes .
T3960 13040-13212 sentence denotes Especially, nerves which control the breathing would be very important, because the respiration system undergoes significant maturation in the first 2–3 weeks after birth.
T3961 13041-13051 RB denotes Especially
T3962 13094-13096 VB denotes be
T3963 13051-13053 , denotes ,
T3964 13053-13059 NNS denotes nerves
T3965 13060-13065 WDT denotes which
T3966 13066-13073 VBP denotes control
T3967 13074-13077 DT denotes the
T3968 13078-13087 NN denotes breathing
T3969 13088-13093 MD denotes would
T3970 13097-13101 RB denotes very
T3971 13102-13111 JJ denotes important
T3972 13111-13113 , denotes ,
T3973 13113-13120 IN denotes because
T3974 13144-13153 VBZ denotes undergoes
T3975 13121-13124 DT denotes the
T3976 13137-13143 NN denotes system
T3977 13125-13136 NN denotes respiration
T3978 13154-13165 JJ denotes significant
T3979 13166-13176 NN denotes maturation
T3980 13177-13179 IN denotes in
T3981 13180-13183 DT denotes the
T3982 13194-13199 NNS denotes weeks
T3983 13184-13189 JJ denotes first
T3984 13190-13191 CD denotes 2
T3985 13192-13193 CD denotes 3
T3986 13191-13192 SYM denotes –
T3987 13200-13205 IN denotes after
T3988 13206-13211 NN denotes birth
T3989 13211-13212 . denotes .
T3990 13212-13410 sentence denotes For example, the myelin-deficient (MD) rat, which carries a point mutation in the proteolipid protein (PLP) gene, exhibits ataxia, tremor, dysmyelination and dies at approximately postnatal day 21.
T3991 13213-13216 IN denotes For
T3992 13327-13335 VBZ denotes exhibits
T3993 13217-13224 NN denotes example
T3994 13224-13226 , denotes ,
T3995 13226-13229 DT denotes the
T3996 13252-13255 NN denotes rat
T3997 13230-13236 NN denotes myelin
T3998 13237-13246 JJ denotes deficient
T3999 13236-13237 HYPH denotes -
T4000 13247-13248 -LRB- denotes (
T4001 13248-13250 JJ denotes MD
T4002 13250-13251 -RRB- denotes )
T4003 13255-13257 , denotes ,
T4004 13257-13262 WDT denotes which
T4005 13263-13270 VBZ denotes carries
T4006 13271-13272 DT denotes a
T4007 13279-13287 NN denotes mutation
T4008 13273-13278 NN denotes point
T4009 13288-13290 IN denotes in
T4010 13291-13294 DT denotes the
T4011 13321-13325 NN denotes gene
T4012 13295-13306 NN denotes proteolipid
T4013 13307-13314 NN denotes protein
T4014 13315-13316 -LRB- denotes (
T4015 13316-13319 NN denotes PLP
T4016 13319-13320 -RRB- denotes )
T4017 13325-13327 , denotes ,
T4018 13336-13342 NN denotes ataxia
T4019 13352-13366 NN denotes dysmyelination
T4020 13342-13344 , denotes ,
T4021 13344-13350 NN denotes tremor
T4022 13350-13352 , denotes ,
T4023 13367-13370 CC denotes and
T4024 13371-13375 VBZ denotes dies
T4025 13376-13378 IN denotes at
T4026 13379-13392 RB denotes approximately
T4027 13403-13406 NN denotes day
T4028 13393-13402 JJ denotes postnatal
T4029 13407-13409 CD denotes 21
T4030 13409-13410 . denotes .
T4031 13410-13557 sentence denotes In MD rat, early death is caused by the dysfunction of the brain stem which is essential for autonomic control of respiration during hypoxia [24].
T4032 13411-13413 IN denotes In
T4033 13437-13443 VBN denotes caused
T4034 13414-13416 NN denotes MD
T4035 13417-13420 NN denotes rat
T4036 13420-13422 , denotes ,
T4037 13422-13427 JJ denotes early
T4038 13428-13433 NN denotes death
T4039 13434-13436 VBZ denotes is
T4040 13444-13446 IN denotes by
T4041 13447-13450 DT denotes the
T4042 13451-13462 NN denotes dysfunction
T4043 13463-13465 IN denotes of
T4044 13466-13469 DT denotes the
T4045 13476-13480 NN denotes stem
T4046 13470-13475 NN denotes brain
T4047 13481-13486 WDT denotes which
T4048 13487-13489 VBZ denotes is
T4049 13490-13499 JJ denotes essential
T4050 13500-13503 IN denotes for
T4051 13504-13513 JJ denotes autonomic
T4052 13514-13521 NN denotes control
T4053 13522-13524 IN denotes of
T4054 13525-13536 NN denotes respiration
T4055 13537-13543 IN denotes during
T4056 13544-13551 NN denotes hypoxia
T4057 13552-13553 -LRB- denotes [
T4058 13553-13555 CD denotes 24
T4059 13555-13556 -RRB- denotes ]
T4060 13556-13557 . denotes .
T4061 13557-13636 sentence denotes Further study is needed to verify the cause of death in ADAM22-deficient mice.
T4062 13558-13565 JJ denotes Further
T4063 13566-13571 NN denotes study
T4064 13575-13581 VBN denotes needed
T4065 13572-13574 VBZ denotes is
T4066 13582-13584 TO denotes to
T4067 13585-13591 VB denotes verify
T4068 13592-13595 DT denotes the
T4069 13596-13601 NN denotes cause
T4070 13602-13604 IN denotes of
T4071 13605-13610 NN denotes death
T4072 13611-13613 IN denotes in
T4073 13614-13620 NN denotes ADAM22
T4074 13621-13630 JJ denotes deficient
T4075 13620-13621 HYPH denotes -
T4076 13631-13635 NNS denotes mice
T4077 13635-13636 . denotes .
T4078 13636-13724 sentence denotes Another interesting phenotype observed in ADAM22-deficient mice was hypomyelinogenesis.
T4079 13637-13644 DT denotes Another
T4080 13657-13666 NN denotes phenotype
T4081 13645-13656 JJ denotes interesting
T4082 13701-13704 VBD denotes was
T4083 13667-13675 VBN denotes observed
T4084 13676-13678 IN denotes in
T4085 13679-13685 NN denotes ADAM22
T4086 13686-13695 JJ denotes deficient
T4087 13685-13686 HYPH denotes -
T4088 13696-13700 NNS denotes mice
T4089 13705-13723 NN denotes hypomyelinogenesis
T4090 13723-13724 . denotes .
T4091 13724-13821 sentence denotes Histopathological analysis revealed marked hypomyelination in the sciatic and trigeminal nerves.
T4092 13725-13742 JJ denotes Histopathological
T4093 13743-13751 NN denotes analysis
T4094 13752-13760 VBD denotes revealed
T4095 13761-13767 JJ denotes marked
T4096 13768-13783 NN denotes hypomyelination
T4097 13784-13786 IN denotes in
T4098 13787-13790 DT denotes the
T4099 13814-13820 NNS denotes nerves
T4100 13791-13798 JJ denotes sciatic
T4101 13799-13802 CC denotes and
T4102 13803-13813 JJ denotes trigeminal
T4103 13820-13821 . denotes .
T4104 13821-13892 sentence denotes However, myelin formation in the spinal cord and the brain was normal.
T4105 13822-13829 RB denotes However
T4106 13881-13884 VBD denotes was
T4107 13829-13831 , denotes ,
T4108 13831-13837 NN denotes myelin
T4109 13838-13847 NN denotes formation
T4110 13848-13850 IN denotes in
T4111 13851-13854 DT denotes the
T4112 13862-13866 NN denotes cord
T4113 13855-13861 JJ denotes spinal
T4114 13867-13870 CC denotes and
T4115 13871-13874 DT denotes the
T4116 13875-13880 NN denotes brain
T4117 13885-13891 JJ denotes normal
T4118 13891-13892 . denotes .
T4119 13892-14047 sentence denotes It was therefore evident that hypomyelinogenesis occurred specifically in the peripheral nerves in the knockout mice, suggesting Schwann cell dysfunction.
T4120 13893-13895 PRP denotes It
T4121 13896-13899 VBD denotes was
T4122 13900-13909 RB denotes therefore
T4123 13910-13917 JJ denotes evident
T4124 13918-13922 IN denotes that
T4125 13942-13950 VBD denotes occurred
T4126 13923-13941 NN denotes hypomyelinogenesis
T4127 13951-13963 RB denotes specifically
T4128 13964-13966 IN denotes in
T4129 13967-13970 DT denotes the
T4130 13982-13988 NNS denotes nerves
T4131 13971-13981 JJ denotes peripheral
T4132 13989-13991 IN denotes in
T4133 13992-13995 DT denotes the
T4134 14005-14009 NNS denotes mice
T4135 13996-14004 NN denotes knockout
T4136 14009-14011 , denotes ,
T4137 14011-14021 VBG denotes suggesting
T4138 14022-14029 NNP denotes Schwann
T4139 14030-14034 NN denotes cell
T4140 14035-14046 NN denotes dysfunction
T4141 14046-14047 . denotes .
T4142 14047-14204 sentence denotes In the mutant sciatic nerve, the density of pro-myelinating Schwann cells (S100-positive, MBP-negative) was markedly higher than that seen in heterozygotes.
T4143 14048-14050 IN denotes In
T4144 14152-14155 VBD denotes was
T4145 14051-14054 DT denotes the
T4146 14070-14075 NN denotes nerve
T4147 14055-14061 NN denotes mutant
T4148 14062-14069 JJ denotes sciatic
T4149 14075-14077 , denotes ,
T4150 14077-14080 DT denotes the
T4151 14081-14088 NN denotes density
T4152 14089-14091 IN denotes of
T4153 14092-14107 VBG denotes pro-myelinating
T4154 14116-14121 NNS denotes cells
T4155 14108-14115 NNP denotes Schwann
T4156 14122-14123 -LRB- denotes (
T4157 14142-14150 JJ denotes negative
T4158 14123-14127 NN denotes S100
T4159 14128-14136 JJ denotes positive
T4160 14127-14128 HYPH denotes -
T4161 14136-14138 , denotes ,
T4162 14138-14141 NN denotes MBP
T4163 14141-14142 HYPH denotes -
T4164 14150-14151 -RRB- denotes )
T4165 14156-14164 RB denotes markedly
T4166 14165-14171 JJR denotes higher
T4167 14172-14176 IN denotes than
T4168 14177-14181 DT denotes that
T4169 14182-14186 VBN denotes seen
T4170 14187-14189 IN denotes in
T4171 14190-14203 NNS denotes heterozygotes
T4172 14203-14204 . denotes .
T4173 14204-14389 sentence denotes Higher pro-myelinating Schwann cell density and marked delay in myelin formation indicate that ADAM22 plays an important role in Schwann cell differentiation, but not in proliferation.
T4174 14205-14211 JJR denotes Higher
T4175 14241-14248 NN denotes density
T4176 14212-14227 VBG denotes pro-myelinating
T4177 14236-14240 NN denotes cell
T4178 14228-14235 NNP denotes Schwann
T4179 14286-14294 VBP denotes indicate
T4180 14249-14252 CC denotes and
T4181 14253-14259 JJ denotes marked
T4182 14260-14265 NN denotes delay
T4183 14266-14268 IN denotes in
T4184 14269-14275 NN denotes myelin
T4185 14276-14285 NN denotes formation
T4186 14295-14299 IN denotes that
T4187 14307-14312 VBZ denotes plays
T4188 14300-14306 NN denotes ADAM22
T4189 14313-14315 DT denotes an
T4190 14326-14330 NN denotes role
T4191 14316-14325 JJ denotes important
T4192 14331-14333 IN denotes in
T4193 14334-14341 NNP denotes Schwann
T4194 14342-14346 NN denotes cell
T4195 14347-14362 NN denotes differentiation
T4196 14362-14364 , denotes ,
T4197 14364-14367 CC denotes but
T4198 14368-14371 RB denotes not
T4199 14372-14374 IN denotes in
T4200 14375-14388 NN denotes proliferation
T4201 14388-14389 . denotes .
T4202 14389-14484 sentence denotes Investigations on why hypomyelinogenesis occurred in ADAM22-deficient mice centre on integrin.
T4203 14390-14404 NNS denotes Investigations
T4204 14465-14471 VBP denotes centre
T4205 14405-14407 IN denotes on
T4206 14408-14411 WRB denotes why
T4207 14431-14439 VBD denotes occurred
T4208 14412-14430 NN denotes hypomyelinogenesis
T4209 14440-14442 IN denotes in
T4210 14443-14449 NN denotes ADAM22
T4211 14450-14459 JJ denotes deficient
T4212 14449-14450 HYPH denotes -
T4213 14460-14464 NNS denotes mice
T4214 14472-14474 IN denotes on
T4215 14475-14483 NN denotes integrin
T4216 14483-14484 . denotes .
T4217 14484-14711 sentence denotes This is because several ADAMs have been shown to interact with integrins via disintegrin domains [9], and it has been suggested that alpha-6 beta-1 integrin on the Schwann cell plays an important role in myelin formation [25].
T4218 14485-14489 DT denotes This
T4219 14490-14492 VBZ denotes is
T4220 14493-14500 IN denotes because
T4221 14525-14530 VBN denotes shown
T4222 14501-14508 JJ denotes several
T4223 14509-14514 NNS denotes ADAMs
T4224 14515-14519 VBP denotes have
T4225 14520-14524 VBN denotes been
T4226 14531-14533 TO denotes to
T4227 14534-14542 VB denotes interact
T4228 14543-14547 IN denotes with
T4229 14548-14557 NNS denotes integrins
T4230 14558-14561 IN denotes via
T4231 14562-14573 NN denotes disintegrin
T4232 14574-14581 NNS denotes domains
T4233 14582-14583 -LRB- denotes [
T4234 14583-14584 CD denotes 9
T4235 14584-14585 -RRB- denotes ]
T4236 14585-14587 , denotes ,
T4237 14587-14590 CC denotes and
T4238 14591-14593 PRP denotes it
T4239 14603-14612 VBN denotes suggested
T4240 14594-14597 VBZ denotes has
T4241 14598-14602 VBN denotes been
T4242 14613-14617 IN denotes that
T4243 14662-14667 VBZ denotes plays
T4244 14618-14623 NN denotes alpha
T4245 14633-14641 NN denotes integrin
T4246 14623-14624 HYPH denotes -
T4247 14624-14625 CD denotes 6
T4248 14626-14630 NN denotes beta
T4249 14630-14631 HYPH denotes -
T4250 14631-14632 CD denotes 1
T4251 14642-14644 IN denotes on
T4252 14645-14648 DT denotes the
T4253 14657-14661 NN denotes cell
T4254 14649-14656 NNP denotes Schwann
T4255 14668-14670 DT denotes an
T4256 14681-14685 NN denotes role
T4257 14671-14680 JJ denotes important
T4258 14686-14688 IN denotes in
T4259 14689-14695 NN denotes myelin
T4260 14696-14705 NN denotes formation
T4261 14706-14707 -LRB- denotes [
T4262 14707-14709 CD denotes 25
T4263 14709-14710 -RRB- denotes ]
T4264 14710-14711 . denotes .
T4265 14711-14980 sentence denotes In addition, laminin-2 is a key player in peripheral myelin formation, because laminin-2 deficiency causes dysmyelination in mice and humans, and its receptors, alpha-6 beta-1 integrin and dystroglycan, are also shown to be deeply involved in myelin formation [26,27].
T4266 14712-14714 IN denotes In
T4267 14735-14737 VBZ denotes is
T4268 14715-14723 NN denotes addition
T4269 14723-14725 , denotes ,
T4270 14725-14732 NN denotes laminin
T4271 14732-14733 HYPH denotes -
T4272 14733-14734 CD denotes 2
T4273 14738-14739 DT denotes a
T4274 14744-14750 NN denotes player
T4275 14740-14743 JJ denotes key
T4276 14751-14753 IN denotes in
T4277 14754-14764 JJ denotes peripheral
T4278 14772-14781 NN denotes formation
T4279 14765-14771 NN denotes myelin
T4280 14781-14783 , denotes ,
T4281 14783-14790 IN denotes because
T4282 14812-14818 VBZ denotes causes
T4283 14791-14798 NN denotes laminin
T4284 14801-14811 NN denotes deficiency
T4285 14798-14799 HYPH denotes -
T4286 14799-14800 CD denotes 2
T4287 14819-14833 NN denotes dysmyelination
T4288 14834-14836 IN denotes in
T4289 14837-14841 NNS denotes mice
T4290 14842-14845 CC denotes and
T4291 14846-14852 NNS denotes humans
T4292 14852-14854 , denotes ,
T4293 14854-14857 CC denotes and
T4294 14858-14861 PRP$ denotes its
T4295 14862-14871 NNS denotes receptors
T4296 14924-14929 VBN denotes shown
T4297 14871-14873 , denotes ,
T4298 14873-14878 NN denotes alpha
T4299 14888-14896 NN denotes integrin
T4300 14878-14879 HYPH denotes -
T4301 14879-14880 CD denotes 6
T4302 14881-14885 NN denotes beta
T4303 14885-14886 HYPH denotes -
T4304 14886-14887 CD denotes 1
T4305 14897-14900 CC denotes and
T4306 14901-14913 NN denotes dystroglycan
T4307 14913-14915 , denotes ,
T4308 14915-14918 VBP denotes are
T4309 14919-14923 RB denotes also
T4310 14930-14932 TO denotes to
T4311 14943-14951 VBN denotes involved
T4312 14933-14935 VB denotes be
T4313 14936-14942 RB denotes deeply
T4314 14952-14954 IN denotes in
T4315 14955-14961 NN denotes myelin
T4316 14962-14971 NN denotes formation
T4317 14972-14973 -LRB- denotes [
T4318 14976-14978 CD denotes 27
T4319 14973-14975 CD denotes 26
T4320 14975-14976 , denotes ,
T4321 14978-14979 -RRB- denotes ]
T4322 14979-14980 . denotes .
T4323 14980-15216 sentence denotes The conditional knockout studies of beta-1 integrin and laminin gamma-1 in Schwann cell suggested that both laminin-2 and integrins are essential for segregation of large bundles of axons in the early stage of myelin formation [25,28].
T4324 14981-14984 DT denotes The
T4325 15006-15013 NNS denotes studies
T4326 14985-14996 JJ denotes conditional
T4327 14997-15005 NN denotes knockout
T4328 15069-15078 VBD denotes suggested
T4329 15014-15016 IN denotes of
T4330 15017-15021 NN denotes beta
T4331 15024-15032 NN denotes integrin
T4332 15021-15022 HYPH denotes -
T4333 15022-15023 CD denotes 1
T4334 15033-15036 CC denotes and
T4335 15037-15044 NN denotes laminin
T4336 15045-15050 NN denotes gamma
T4337 15050-15051 HYPH denotes -
T4338 15051-15052 CD denotes 1
T4339 15053-15055 IN denotes in
T4340 15056-15063 NNP denotes Schwann
T4341 15064-15068 NN denotes cell
T4342 15079-15083 IN denotes that
T4343 15113-15116 VBP denotes are
T4344 15084-15088 CC denotes both
T4345 15089-15096 NN denotes laminin
T4346 15096-15097 HYPH denotes -
T4347 15097-15098 CD denotes 2
T4348 15099-15102 CC denotes and
T4349 15103-15112 NNS denotes integrins
T4350 15117-15126 JJ denotes essential
T4351 15127-15130 IN denotes for
T4352 15131-15142 NN denotes segregation
T4353 15143-15145 IN denotes of
T4354 15146-15151 JJ denotes large
T4355 15152-15159 NNS denotes bundles
T4356 15160-15162 IN denotes of
T4357 15163-15168 NNS denotes axons
T4358 15169-15171 IN denotes in
T4359 15172-15175 DT denotes the
T4360 15182-15187 NN denotes stage
T4361 15176-15181 JJ denotes early
T4362 15188-15190 IN denotes of
T4363 15191-15197 NN denotes myelin
T4364 15198-15207 NN denotes formation
T4365 15208-15209 -LRB- denotes [
T4366 15212-15214 CD denotes 28
T4367 15209-15211 CD denotes 25
T4368 15211-15212 , denotes ,
T4369 15214-15215 -RRB- denotes ]
T4370 15215-15216 . denotes .
T4371 15216-15372 sentence denotes In contrast, the phenotype seen in the ADAM22-deficient mice was quite different: for example, unsorted bundles of axons were not seen in nerves and roots.
T4372 15217-15219 IN denotes In
T4373 15278-15281 VBD denotes was
T4374 15220-15228 NN denotes contrast
T4375 15228-15230 , denotes ,
T4376 15230-15233 DT denotes the
T4377 15234-15243 NN denotes phenotype
T4378 15244-15248 VBN denotes seen
T4379 15249-15251 IN denotes in
T4380 15252-15255 DT denotes the
T4381 15273-15277 NNS denotes mice
T4382 15256-15262 NN denotes ADAM22
T4383 15263-15272 JJ denotes deficient
T4384 15262-15263 HYPH denotes -
T4385 15347-15351 VBN denotes seen
T4386 15282-15287 RB denotes quite
T4387 15288-15297 JJ denotes different
T4388 15297-15299 : denotes :
T4389 15299-15302 IN denotes for
T4390 15303-15310 NN denotes example
T4391 15310-15312 , denotes ,
T4392 15312-15320 JJ denotes unsorted
T4393 15321-15328 NNS denotes bundles
T4394 15329-15331 IN denotes of
T4395 15332-15337 NNS denotes axons
T4396 15338-15342 VBD denotes were
T4397 15343-15346 RB denotes not
T4398 15352-15354 IN denotes in
T4399 15355-15361 NNS denotes nerves
T4400 15362-15365 CC denotes and
T4401 15366-15371 NNS denotes roots
T4402 15371-15372 . denotes .
T4403 15372-15493 sentence denotes We assume that ADAM22 partially modulates integrin-mediated signals or is involved at a later stage of myelin formation.
T4404 15373-15375 PRP denotes We
T4405 15376-15382 VBP denotes assume
T4406 15383-15387 IN denotes that
T4407 15405-15414 VBZ denotes modulates
T4408 15388-15394 NN denotes ADAM22
T4409 15395-15404 RB denotes partially
T4410 15415-15423 NN denotes integrin
T4411 15424-15432 VBN denotes mediated
T4412 15423-15424 HYPH denotes -
T4413 15433-15440 NNS denotes signals
T4414 15441-15443 CC denotes or
T4415 15444-15446 VBZ denotes is
T4416 15447-15455 VBN denotes involved
T4417 15456-15458 IN denotes at
T4418 15459-15460 DT denotes a
T4419 15467-15472 NN denotes stage
T4420 15461-15466 JJ denotes later
T4421 15473-15475 IN denotes of
T4422 15476-15482 NN denotes myelin
T4423 15483-15492 NN denotes formation
T4424 15492-15493 . denotes .
T4470 15506-15508 IN denotes In
T4471 15521-15530 VBD denotes generated
T4472 15509-15516 NN denotes summary
T4473 15516-15518 , denotes ,
T4474 15518-15520 PRP denotes we
T4475 15531-15537 NN denotes ADAM22
T4476 15538-15547 JJ denotes deficient
T4477 15537-15538 HYPH denotes -
T4478 15548-15552 NNS denotes mice
T4479 15553-15556 CC denotes and
T4480 15557-15563 VBD denotes proved
T4481 15564-15568 IN denotes that
T4482 15576-15581 VBZ denotes plays
T4483 15569-15575 NN denotes ADAM22
T4484 15582-15584 DT denotes an
T4485 15595-15599 NN denotes role
T4486 15585-15594 JJ denotes essential
T4487 15600-15602 IN denotes in
T4488 15603-15607 CC denotes both
T4489 15612-15615 NN denotes CNS
T4490 15608-15611 DT denotes the
T4491 15616-15619 CC denotes and
T4492 15620-15623 DT denotes the
T4493 15624-15627 NN denotes PNS
T4494 15627-15628 . denotes .
T4495 15628-15748 sentence denotes The results of this study suggest that ADAM22 is involved in human diseases such as epilepsy and peripheral neuropathy.
T4496 15629-15632 DT denotes The
T4497 15633-15640 NNS denotes results
T4498 15655-15662 VBP denotes suggest
T4499 15641-15643 IN denotes of
T4500 15644-15648 DT denotes this
T4501 15649-15654 NN denotes study
T4502 15663-15667 IN denotes that
T4503 15678-15686 VBN denotes involved
T4504 15668-15674 NN denotes ADAM22
T4505 15675-15677 VBZ denotes is
T4506 15687-15689 IN denotes in
T4507 15690-15695 JJ denotes human
T4508 15696-15704 NNS denotes diseases
T4509 15705-15709 JJ denotes such
T4510 15710-15712 IN denotes as
T4511 15713-15721 NN denotes epilepsy
T4512 15722-15725 CC denotes and
T4513 15726-15736 JJ denotes peripheral
T4514 15737-15747 NN denotes neuropathy
T4515 15747-15748 . denotes .
T4516 15748-15852 sentence denotes The human ADAM22 gene is relatively large, at 300 kb in length, and is comprised of more than 30 exons.
T4517 15749-15752 DT denotes The
T4518 15766-15770 NN denotes gene
T4519 15753-15758 JJ denotes human
T4520 15759-15765 NN denotes ADAM22
T4521 15771-15773 VBZ denotes is
T4522 15774-15784 RB denotes relatively
T4523 15785-15790 JJ denotes large
T4524 15790-15792 , denotes ,
T4525 15792-15794 IN denotes at
T4526 15795-15798 CD denotes 300
T4527 15799-15801 NN denotes kb
T4528 15802-15804 IN denotes in
T4529 15805-15811 NN denotes length
T4530 15811-15813 , denotes ,
T4531 15813-15816 CC denotes and
T4532 15817-15819 VBZ denotes is
T4533 15820-15829 VBN denotes comprised
T4534 15830-15832 IN denotes of
T4535 15833-15837 JJR denotes more
T4536 15843-15845 CD denotes 30
T4537 15838-15842 IN denotes than
T4538 15846-15851 NNS denotes exons
T4539 15851-15852 . denotes .
T4540 15852-15991 sentence denotes It is assigned on 7q21, where several chromosomal aberrations that are accompanied by neurological disorders have been identified [29,30].
T4541 15853-15855 PRP denotes It
T4542 15859-15867 VBN denotes assigned
T4543 15856-15858 VBZ denotes is
T4544 15868-15870 IN denotes on
T4545 15871-15875 NN denotes 7q21
T4546 15875-15877 , denotes ,
T4547 15877-15882 WRB denotes where
T4548 15972-15982 VBN denotes identified
T4549 15883-15890 JJ denotes several
T4550 15903-15914 NNS denotes aberrations
T4551 15891-15902 JJ denotes chromosomal
T4552 15915-15919 WDT denotes that
T4553 15924-15935 VBN denotes accompanied
T4554 15920-15923 VBP denotes are
T4555 15936-15938 IN denotes by
T4556 15939-15951 JJ denotes neurological
T4557 15952-15961 NNS denotes disorders
T4558 15962-15966 VBP denotes have
T4559 15967-15971 VBN denotes been
T4560 15983-15984 -LRB- denotes [
T4561 15987-15989 CD denotes 30
T4562 15984-15986 CD denotes 29
T4563 15986-15987 , denotes ,
T4564 15989-15990 -RRB- denotes ]
T4565 15990-15991 . denotes .
T4566 15991-16147 sentence denotes Further detailed molecular function analysis of ADAM22 may lead to progress in uncovering the mechanisms that underlie certain human neurological diseases.
T4567 15992-15999 JJ denotes Further
T4568 16028-16036 NN denotes analysis
T4569 16000-16008 JJ denotes detailed
T4570 16009-16018 JJ denotes molecular
T4571 16019-16027 NN denotes function
T4572 16051-16055 VB denotes lead
T4573 16037-16039 IN denotes of
T4574 16040-16046 NN denotes ADAM22
T4575 16047-16050 MD denotes may
T4576 16056-16058 IN denotes to
T4577 16059-16067 NN denotes progress
T4578 16068-16070 IN denotes in
T4579 16071-16081 VBG denotes uncovering
T4580 16082-16085 DT denotes the
T4581 16086-16096 NNS denotes mechanisms
T4582 16097-16101 WDT denotes that
T4583 16102-16110 VBP denotes underlie
T4584 16111-16118 JJ denotes certain
T4585 16138-16146 NNS denotes diseases
T4586 16119-16124 JJ denotes human
T4587 16125-16137 JJ denotes neurological
T4588 16146-16147 . denotes .
T4651 16158-16170 NN denotes Construction
T4652 16171-16173 IN denotes of
T4653 16174-16176 DT denotes an
T4654 16199-16205 NN denotes vector
T4655 16177-16183 NN denotes Adam22
T4656 16184-16188 NN denotes gene
T4657 16189-16198 VBG denotes targeting
T4658 16188-16189 HYPH denotes -
T4659 16205-16427 sentence denotes The 18-kb genomic DNA fragments covering exons 5, 6, 7, 8 and 9 of the Adam22 gene were amplified from C57BL/6 genomic DNA by PCR using Expand Hi-Fidelity enzyme mix (Roche Diagnostics) and primers designed for each exon.
T4660 16206-16209 DT denotes The
T4661 16228-16237 NNS denotes fragments
T4662 16210-16212 CD denotes 18
T4663 16213-16215 NN denotes kb
T4664 16212-16213 HYPH denotes -
T4665 16216-16223 JJ denotes genomic
T4666 16224-16227 NN denotes DNA
T4667 16294-16303 VBN denotes amplified
T4668 16238-16246 VBG denotes covering
T4669 16247-16252 NNS denotes exons
T4670 16253-16254 CD denotes 5
T4671 16254-16256 , denotes ,
T4672 16256-16257 CD denotes 6
T4673 16257-16259 , denotes ,
T4674 16259-16260 CD denotes 7
T4675 16260-16262 , denotes ,
T4676 16262-16263 CD denotes 8
T4677 16264-16267 CC denotes and
T4678 16268-16269 CD denotes 9
T4679 16270-16272 IN denotes of
T4680 16273-16276 DT denotes the
T4681 16284-16288 NN denotes gene
T4682 16277-16283 NN denotes Adam22
T4683 16289-16293 VBD denotes were
T4684 16304-16308 IN denotes from
T4685 16309-16314 NN denotes C57BL
T4686 16325-16328 NN denotes DNA
T4687 16314-16315 HYPH denotes /
T4688 16315-16316 CD denotes 6
T4689 16317-16324 JJ denotes genomic
T4690 16329-16331 IN denotes by
T4691 16332-16335 NN denotes PCR
T4692 16336-16341 VBG denotes using
T4693 16342-16348 NNP denotes Expand
T4694 16368-16371 NN denotes mix
T4695 16349-16351 NNP denotes Hi
T4696 16352-16360 NNP denotes Fidelity
T4697 16351-16352 HYPH denotes -
T4698 16361-16367 NN denotes enzyme
T4699 16372-16373 -LRB- denotes (
T4700 16379-16390 NNP denotes Diagnostics
T4701 16373-16378 NNP denotes Roche
T4702 16390-16391 -RRB- denotes )
T4703 16392-16395 CC denotes and
T4704 16396-16403 NNS denotes primers
T4705 16404-16412 VBN denotes designed
T4706 16413-16416 IN denotes for
T4707 16417-16421 DT denotes each
T4708 16422-16426 NN denotes exon
T4709 16426-16427 . denotes .
T4710 16427-16540 sentence denotes To generate a mutation in the mouse Adam22 gene, we inserted the PGKneo cassette into exon 8 of the Adam22 gene.
T4711 16428-16430 TO denotes To
T4712 16431-16439 VB denotes generate
T4713 16480-16488 VBD denotes inserted
T4714 16440-16441 DT denotes a
T4715 16442-16450 NN denotes mutation
T4716 16451-16453 IN denotes in
T4717 16454-16457 DT denotes the
T4718 16471-16475 NN denotes gene
T4719 16458-16463 NN denotes mouse
T4720 16464-16470 NN denotes Adam22
T4721 16475-16477 , denotes ,
T4722 16477-16479 PRP denotes we
T4723 16489-16492 DT denotes the
T4724 16500-16508 NN denotes cassette
T4725 16493-16499 NN denotes PGKneo
T4726 16509-16513 IN denotes into
T4727 16514-16518 NN denotes exon
T4728 16519-16520 CD denotes 8
T4729 16521-16523 IN denotes of
T4730 16524-16527 DT denotes the
T4731 16535-16539 NN denotes gene
T4732 16528-16534 NN denotes Adam22
T4733 16539-16540 . denotes .
T4734 16540-16770 sentence denotes The final targeting construct consisted of a 10.5-kb genomic DNA fragment that was interrupted by the insertion of the PGKneo cassette and contained a MC1/TK cassette as a negative selection marker against random integration [5].
T4735 16541-16544 DT denotes The
T4736 16561-16570 NN denotes construct
T4737 16545-16550 JJ denotes final
T4738 16551-16560 NN denotes targeting
T4739 16571-16580 VBD denotes consisted
T4740 16581-16583 IN denotes of
T4741 16584-16585 DT denotes a
T4742 16606-16614 NN denotes fragment
T4743 16586-16590 CD denotes 10.5
T4744 16591-16593 NN denotes kb
T4745 16590-16591 HYPH denotes -
T4746 16594-16601 JJ denotes genomic
T4747 16602-16605 NN denotes DNA
T4748 16615-16619 WDT denotes that
T4749 16624-16635 VBN denotes interrupted
T4750 16620-16623 VBD denotes was
T4751 16636-16638 IN denotes by
T4752 16639-16642 DT denotes the
T4753 16643-16652 NN denotes insertion
T4754 16653-16655 IN denotes of
T4755 16656-16659 DT denotes the
T4756 16667-16675 NN denotes cassette
T4757 16660-16666 NN denotes PGKneo
T4758 16676-16679 CC denotes and
T4759 16680-16689 VBD denotes contained
T4760 16690-16691 DT denotes a
T4761 16699-16707 NN denotes cassette
T4762 16692-16695 NN denotes MC1
T4763 16696-16698 NN denotes TK
T4764 16695-16696 HYPH denotes /
T4765 16708-16710 IN denotes as
T4766 16711-16712 DT denotes a
T4767 16732-16738 NN denotes marker
T4768 16713-16721 JJ denotes negative
T4769 16722-16731 NN denotes selection
T4770 16739-16746 IN denotes against
T4771 16747-16753 JJ denotes random
T4772 16754-16765 NN denotes integration
T4773 16766-16767 -LRB- denotes [
T4774 16767-16768 CD denotes 5
T4775 16768-16769 -RRB- denotes ]
T4776 16769-16770 . denotes .
T4851 16772-16782 NN denotes Generation
T4852 16783-16785 IN denotes of
T4853 16786-16792 NN denotes Adam22
T4854 16793-16802 JJ denotes deficient
T4855 16803-16807 NNS denotes mice
T4856 16807-16899 sentence denotes The linearised targeting vector was electroporated into TT2 embryonic stem (ES) cells [31].
T4857 16808-16811 DT denotes The
T4858 16833-16839 NN denotes vector
T4859 16812-16822 VBN denotes linearised
T4860 16823-16832 NN denotes targeting
T4861 16844-16858 VBN denotes electroporated
T4862 16840-16843 VBD denotes was
T4863 16859-16863 IN denotes into
T4864 16864-16867 NN denotes TT2
T4865 16888-16893 NNS denotes cells
T4866 16868-16877 JJ denotes embryonic
T4867 16878-16882 NN denotes stem
T4868 16883-16884 -LRB- denotes (
T4869 16884-16886 NN denotes ES
T4870 16886-16887 -RRB- denotes )
T4871 16894-16895 -LRB- denotes [
T4872 16895-16897 CD denotes 31
T4873 16897-16898 -RRB- denotes ]
T4874 16898-16899 . denotes .
T4875 16899-16945 sentence denotes Homologous recombinants were selected by PCR.
T4876 16900-16910 JJ denotes Homologous
T4877 16911-16923 NNS denotes recombinants
T4878 16929-16937 VBN denotes selected
T4879 16924-16928 VBD denotes were
T4880 16938-16940 IN denotes by
T4881 16941-16944 NN denotes PCR
T4882 16944-16945 . denotes .
T4883 16945-17190 sentence denotes The extracted genomic DNA from each clone was amplified using the forward primer AGN2: 5'-GCCTGCTTGCCGAATATCATGGTGGAAAAT-3' in the PGKneo cassette, and the reverse primer MFP065R: 5'-ACTATTTCTGTGATGAGGGCACAGCATC-3' outside the targeting vector.
T4884 16946-16949 DT denotes The
T4885 16968-16971 NN denotes DNA
T4886 16950-16959 VBN denotes extracted
T4887 16960-16967 JJ denotes genomic
T4888 16992-17001 VBN denotes amplified
T4889 16972-16976 IN denotes from
T4890 16977-16981 DT denotes each
T4891 16982-16987 NN denotes clone
T4892 16988-16991 VBD denotes was
T4893 17002-17007 VBG denotes using
T4894 17008-17011 DT denotes the
T4895 17020-17026 NN denotes primer
T4896 17012-17019 JJ denotes forward
T4897 17027-17031 NN denotes AGN2
T4898 17031-17033 : denotes :
T4899 17033-17034 CD denotes 5
T4900 17036-17066 NN denotes GCCTGCTTGCCGAATATCATGGTGGAAAAT
T4901 17034-17035 SYM denotes '
T4902 17035-17036 HYPH denotes -
T4903 17066-17067 HYPH denotes -
T4904 17067-17068 CD denotes 3
T4905 17068-17069 SYM denotes '
T4906 17070-17072 IN denotes in
T4907 17073-17076 DT denotes the
T4908 17084-17092 NN denotes cassette
T4909 17077-17083 NN denotes PGKneo
T4910 17092-17094 , denotes ,
T4911 17094-17097 CC denotes and
T4912 17098-17101 DT denotes the
T4913 17110-17116 NN denotes primer
T4914 17102-17109 JJ denotes reverse
T4915 17117-17124 NN denotes MFP065R
T4916 17124-17126 : denotes :
T4917 17126-17127 CD denotes 5
T4918 17129-17157 NN denotes ACTATTTCTGTGATGAGGGCACAGCATC
T4919 17127-17128 SYM denotes '
T4920 17128-17129 HYPH denotes -
T4921 17157-17158 HYPH denotes -
T4922 17158-17159 CD denotes 3
T4923 17159-17160 SYM denotes '
T4924 17161-17168 IN denotes outside
T4925 17169-17172 DT denotes the
T4926 17183-17189 NN denotes vector
T4927 17173-17182 NN denotes targeting
T4928 17189-17190 . denotes .
T4929 17190-17320 sentence denotes Homologous recombined DNA was efficiently amplified by 35 cycles of the following amplification steps (94°C-30 s and 68°C-5 min).
T4930 17191-17201 JJ denotes Homologous
T4931 17213-17216 NN denotes DNA
T4932 17202-17212 JJ denotes recombined
T4933 17233-17242 VBN denotes amplified
T4934 17217-17220 VBD denotes was
T4935 17221-17232 RB denotes efficiently
T4936 17243-17245 IN denotes by
T4937 17246-17248 CD denotes 35
T4938 17249-17255 NNS denotes cycles
T4939 17256-17258 IN denotes of
T4940 17259-17262 DT denotes the
T4941 17287-17292 NNS denotes steps
T4942 17263-17272 VBG denotes following
T4943 17273-17286 NN denotes amplification
T4944 17293-17294 -LRB- denotes (
T4945 17294-17296 CD denotes 94
T4946 17296-17298 NN denotes °C
T4947 17298-17299 HYPH denotes -
T4948 17299-17301 CD denotes 30
T4949 17302-17303 NN denotes s
T4950 17304-17307 CC denotes and
T4951 17308-17310 CD denotes 68
T4952 17310-17312 NN denotes °C
T4953 17312-17313 HYPH denotes -
T4954 17313-17314 CD denotes 5
T4955 17315-17318 NN denotes min
T4956 17318-17319 -RRB- denotes )
T4957 17319-17320 . denotes .
T4958 17320-17377 sentence denotes The targeting efficiency of this construct was about 4%.
T4959 17321-17324 DT denotes The
T4960 17335-17345 NN denotes efficiency
T4961 17325-17334 NN denotes targeting
T4962 17364-17367 VBD denotes was
T4963 17346-17348 IN denotes of
T4964 17349-17353 DT denotes this
T4965 17354-17363 NN denotes construct
T4966 17368-17373 RB denotes about
T4967 17374-17375 CD denotes 4
T4968 17375-17376 NN denotes %
T4969 17376-17377 . denotes .
T4970 17377-17476 sentence denotes Correctly targeted ES clones were injected into fertilized ICR mouse eggs at the eight-cell stage.
T4971 17378-17387 RB denotes Correctly
T4972 17388-17396 VBN denotes targeted
T4973 17400-17406 NNS denotes clones
T4974 17397-17399 NN denotes ES
T4975 17412-17420 VBN denotes injected
T4976 17407-17411 VBD denotes were
T4977 17421-17425 IN denotes into
T4978 17426-17436 VBN denotes fertilized
T4979 17447-17451 NNS denotes eggs
T4980 17437-17440 NN denotes ICR
T4981 17441-17446 NN denotes mouse
T4982 17452-17454 IN denotes at
T4983 17455-17458 DT denotes the
T4984 17470-17475 NN denotes stage
T4985 17459-17464 CD denotes eight
T4986 17465-17469 NN denotes cell
T4987 17464-17465 HYPH denotes -
T4988 17475-17476 . denotes .
T4989 17476-17620 sentence denotes The resulting male chimeras were mated with C57BL/6N females, and heterozygous male and female mice were interbred to generate homozygous mice.
T4990 17477-17480 DT denotes The
T4991 17496-17504 NNS denotes chimeras
T4992 17481-17490 VBG denotes resulting
T4993 17491-17495 JJ denotes male
T4994 17510-17515 VBN denotes mated
T4995 17505-17509 VBD denotes were
T4996 17516-17520 IN denotes with
T4997 17521-17526 NN denotes C57BL
T4998 17530-17537 NNS denotes females
T4999 17526-17527 HYPH denotes /
T5000 17527-17529 CD denotes 6N
T5001 17537-17539 , denotes ,
T5002 17539-17542 CC denotes and
T5003 17543-17555 JJ denotes heterozygous
T5004 17572-17576 NNS denotes mice
T5005 17556-17560 JJ denotes male
T5006 17561-17564 CC denotes and
T5007 17565-17571 JJ denotes female
T5008 17582-17591 VBN denotes interbred
T5009 17577-17581 VBD denotes were
T5010 17592-17594 TO denotes to
T5011 17595-17603 VB denotes generate
T5012 17604-17614 JJ denotes homozygous
T5013 17615-17619 NNS denotes mice
T5014 17619-17620 . denotes .
T5015 17620-17701 sentence denotes The ataxic phenotypes of homozygous mice were observed in two independent lines.
T5016 17621-17624 DT denotes The
T5017 17632-17642 NNS denotes phenotypes
T5018 17625-17631 JJ denotes ataxic
T5019 17667-17675 VBN denotes observed
T5020 17643-17645 IN denotes of
T5021 17646-17656 JJ denotes homozygous
T5022 17657-17661 NNS denotes mice
T5023 17662-17666 VBD denotes were
T5024 17676-17678 IN denotes in
T5025 17679-17682 CD denotes two
T5026 17695-17700 NNS denotes lines
T5027 17683-17694 JJ denotes independent
T5028 17700-17701 . denotes .
T5055 17703-17711 NNP denotes Southern
T5056 17712-17716 NN denotes blot
T5057 17717-17725 NN denotes analysis
T5058 17725-17822 sentence denotes Mouse genomic DNA used for Southern blot analysis was extracted from the liver of each genotype.
T5059 17726-17731 NN denotes Mouse
T5060 17740-17743 NN denotes DNA
T5061 17732-17739 JJ denotes genomic
T5062 17780-17789 VBN denotes extracted
T5063 17744-17748 VBN denotes used
T5064 17749-17752 IN denotes for
T5065 17753-17761 NNP denotes Southern
T5066 17762-17766 NN denotes blot
T5067 17767-17775 NN denotes analysis
T5068 17776-17779 VBD denotes was
T5069 17790-17794 IN denotes from
T5070 17795-17798 DT denotes the
T5071 17799-17804 NN denotes liver
T5072 17805-17807 IN denotes of
T5073 17808-17812 DT denotes each
T5074 17813-17821 NN denotes genotype
T5075 17821-17822 . denotes .
T5076 17822-17960 sentence denotes BamHI-digested genomic DNA was probed with the [32P]-labelled 0.4-kb SpeI-BamHI genomic fragment, which is located between exons 8 and 9.
T5077 17823-17828 NN denotes BamHI
T5078 17829-17837 VBN denotes digested
T5079 17828-17829 HYPH denotes -
T5080 17846-17849 NN denotes DNA
T5081 17838-17845 JJ denotes genomic
T5082 17854-17860 VBN denotes probed
T5083 17850-17853 VBD denotes was
T5084 17861-17865 IN denotes with
T5085 17866-17869 DT denotes the
T5086 17911-17919 NN denotes fragment
T5087 17870-17871 -LRB- denotes [
T5088 17871-17874 CD denotes 32P
T5089 17876-17884 VBN denotes labelled
T5090 17874-17875 -RRB- denotes ]
T5091 17875-17876 HYPH denotes -
T5092 17885-17888 CD denotes 0.4
T5093 17889-17891 NN denotes kb
T5094 17888-17889 HYPH denotes -
T5095 17892-17896 NN denotes SpeI
T5096 17897-17902 NN denotes BamHI
T5097 17896-17897 HYPH denotes -
T5098 17903-17910 JJ denotes genomic
T5099 17919-17921 , denotes ,
T5100 17921-17926 WDT denotes which
T5101 17930-17937 VBN denotes located
T5102 17927-17929 VBZ denotes is
T5103 17938-17945 IN denotes between
T5104 17946-17951 NNS denotes exons
T5105 17952-17953 CD denotes 8
T5106 17954-17957 CC denotes and
T5107 17958-17959 CD denotes 9
T5108 17959-17960 . denotes .
T5223 17962-17970 NN denotes Antibody
T5224 17971-17981 NN denotes production
T5225 17981-18160 sentence denotes His-tagged and MBP-fused recombinant protein containing cytoplasmic domain (cp) of human ADAM22 isoform 1 (position: 756–906, 151 amino acids in length) were produced in E. coli.
T5226 17982-17985 NN denotes His
T5227 17986-17992 VBN denotes tagged
T5228 17985-17986 HYPH denotes -
T5229 18019-18026 NN denotes protein
T5230 17993-17996 CC denotes and
T5231 17997-18000 NN denotes MBP
T5232 18001-18006 VBN denotes fused
T5233 18000-18001 HYPH denotes -
T5234 18007-18018 JJ denotes recombinant
T5235 18140-18148 VBN denotes produced
T5236 18027-18037 VBG denotes containing
T5237 18038-18049 JJ denotes cytoplasmic
T5238 18050-18056 NN denotes domain
T5239 18057-18058 -LRB- denotes (
T5240 18058-18060 NN denotes cp
T5241 18060-18061 -RRB- denotes )
T5242 18062-18064 IN denotes of
T5243 18065-18070 JJ denotes human
T5244 18078-18085 NN denotes isoform
T5245 18071-18077 NN denotes ADAM22
T5246 18086-18087 CD denotes 1
T5247 18088-18089 -LRB- denotes (
T5248 18118-18123 NNS denotes acids
T5249 18089-18097 NN denotes position
T5250 18097-18099 : denotes :
T5251 18099-18102 CD denotes 756
T5252 18103-18106 CD denotes 906
T5253 18102-18103 SYM denotes –
T5254 18106-18108 , denotes ,
T5255 18108-18111 CD denotes 151
T5256 18112-18117 NN denotes amino
T5257 18124-18126 IN denotes in
T5258 18127-18133 NN denotes length
T5259 18133-18134 -RRB- denotes )
T5260 18135-18139 VBD denotes were
T5261 18149-18151 IN denotes in
T5262 18152-18154 NNP denotes E.
T5263 18155-18159 NNP denotes coli
T5264 18159-18160 . denotes .
T5265 18160-18279 sentence denotes His-tagged ADAM22-cp protein was purified in denatured condition using Ni-NTA resin (Qiagen GmbH) and dialysed in PBS.
T5266 18161-18164 NN denotes His
T5267 18165-18171 VBN denotes tagged
T5268 18164-18165 HYPH denotes -
T5269 18182-18189 NN denotes protein
T5270 18172-18178 NN denotes ADAM22
T5271 18179-18181 NN denotes cp
T5272 18178-18179 HYPH denotes -
T5273 18194-18202 VBN denotes purified
T5274 18190-18193 VBD denotes was
T5275 18203-18205 IN denotes in
T5276 18206-18215 VBN denotes denatured
T5277 18216-18225 NN denotes condition
T5278 18226-18231 VBG denotes using
T5279 18232-18234 NN denotes Ni
T5280 18235-18238 NN denotes NTA
T5281 18234-18235 HYPH denotes -
T5282 18239-18244 NN denotes resin
T5283 18245-18246 -LRB- denotes (
T5284 18253-18257 NNP denotes GmbH
T5285 18246-18252 NNP denotes Qiagen
T5286 18257-18258 -RRB- denotes )
T5287 18259-18262 CC denotes and
T5288 18263-18271 VBN denotes dialysed
T5289 18272-18274 IN denotes in
T5290 18275-18278 NN denotes PBS
T5291 18278-18279 . denotes .
T5292 18279-18430 sentence denotes Precipitated protein was recovered and was mixed with Freund's complete adjuvant (Invitrogen), then, the mixture was used for immunization of rabbits.
T5293 18280-18292 VBN denotes Precipitated
T5294 18293-18300 NN denotes protein
T5295 18305-18314 VBN denotes recovered
T5296 18301-18304 VBD denotes was
T5297 18397-18401 VBN denotes used
T5298 18315-18318 CC denotes and
T5299 18319-18322 VBD denotes was
T5300 18323-18328 VBN denotes mixed
T5301 18329-18333 IN denotes with
T5302 18334-18340 NNP denotes Freund
T5303 18352-18360 NN denotes adjuvant
T5304 18340-18342 POS denotes 's
T5305 18343-18351 JJ denotes complete
T5306 18361-18362 -LRB- denotes (
T5307 18362-18372 NNP denotes Invitrogen
T5308 18372-18373 -RRB- denotes )
T5309 18373-18375 , denotes ,
T5310 18375-18379 RB denotes then
T5311 18379-18381 , denotes ,
T5312 18381-18384 DT denotes the
T5313 18385-18392 NN denotes mixture
T5314 18393-18396 VBD denotes was
T5315 18402-18405 IN denotes for
T5316 18406-18418 NN denotes immunization
T5317 18419-18421 IN denotes of
T5318 18422-18429 NNS denotes rabbits
T5319 18429-18430 . denotes .
T5320 18430-18579 sentence denotes The antiserum raised against the His-tagged ADAM22-cp protein was incubated with MBP-fused ADAM22-cp protein coupled to Affi-Gel 10 beads (Bio-Rad).
T5321 18431-18434 DT denotes The
T5322 18435-18444 NN denotes antiserum
T5323 18497-18506 VBN denotes incubated
T5324 18445-18451 VBN denotes raised
T5325 18452-18459 IN denotes against
T5326 18460-18463 DT denotes the
T5327 18485-18492 NN denotes protein
T5328 18464-18467 NN denotes His
T5329 18468-18474 VBN denotes tagged
T5330 18467-18468 HYPH denotes -
T5331 18475-18481 NN denotes ADAM22
T5332 18482-18484 NN denotes cp
T5333 18481-18482 HYPH denotes -
T5334 18493-18496 VBD denotes was
T5335 18507-18511 IN denotes with
T5336 18512-18515 NN denotes MBP
T5337 18516-18521 VBN denotes fused
T5338 18515-18516 HYPH denotes -
T5339 18532-18539 NN denotes protein
T5340 18522-18528 NN denotes ADAM22
T5341 18529-18531 NN denotes cp
T5342 18528-18529 HYPH denotes -
T5343 18540-18547 VBN denotes coupled
T5344 18548-18550 IN denotes to
T5345 18551-18555 NN denotes Affi
T5346 18556-18559 NN denotes Gel
T5347 18555-18556 HYPH denotes -
T5348 18563-18568 NNS denotes beads
T5349 18560-18562 CD denotes 10
T5350 18569-18570 -LRB- denotes (
T5351 18574-18577 NNP denotes Rad
T5352 18570-18573 NNP denotes Bio
T5353 18573-18574 HYPH denotes -
T5354 18577-18578 -RRB- denotes )
T5355 18578-18579 . denotes .
T5356 18579-18722 sentence denotes Beads with bound antibodies were washed in PBS, and the bound antibodies were eluted with 100 mM glycine, pH 3.0, and neutralized immediately.
T5357 18580-18585 NNS denotes Beads
T5358 18613-18619 VBN denotes washed
T5359 18586-18590 IN denotes with
T5360 18591-18596 VBN denotes bound
T5361 18597-18607 NNS denotes antibodies
T5362 18608-18612 VBD denotes were
T5363 18620-18622 IN denotes in
T5364 18623-18626 NN denotes PBS
T5365 18626-18628 , denotes ,
T5366 18628-18631 CC denotes and
T5367 18632-18635 DT denotes the
T5368 18642-18652 NNS denotes antibodies
T5369 18636-18641 VBN denotes bound
T5370 18658-18664 VBN denotes eluted
T5371 18653-18657 VBD denotes were
T5372 18665-18669 IN denotes with
T5373 18670-18673 CD denotes 100
T5374 18674-18676 NN denotes mM
T5375 18677-18684 NN denotes glycine
T5376 18684-18686 , denotes ,
T5377 18686-18688 NN denotes pH
T5378 18689-18692 CD denotes 3.0
T5379 18692-18694 , denotes ,
T5380 18694-18697 CC denotes and
T5381 18698-18709 VBN denotes neutralized
T5382 18710-18721 RB denotes immediately
T5383 18721-18722 . denotes .
T5384 18722-18837 sentence denotes Using the affinity purified antibody, both human and mouse ADAM22 proteins were detected by Western blot analysis.
T5385 18723-18728 VBG denotes Using
T5386 18803-18811 VBN denotes detected
T5387 18729-18732 DT denotes the
T5388 18751-18759 NN denotes antibody
T5389 18733-18741 NN denotes affinity
T5390 18742-18750 VBN denotes purified
T5391 18759-18761 , denotes ,
T5392 18761-18765 CC denotes both
T5393 18776-18781 NN denotes mouse
T5394 18766-18771 JJ denotes human
T5395 18772-18775 CC denotes and
T5396 18789-18797 NN denotes proteins
T5397 18782-18788 NN denotes ADAM22
T5398 18798-18802 VBD denotes were
T5399 18812-18814 IN denotes by
T5400 18815-18822 NNP denotes Western
T5401 18823-18827 NN denotes blot
T5402 18828-18836 NN denotes analysis
T5403 18836-18837 . denotes .
T5404 18837-18941 sentence denotes However, unfortunately the antibody was not suitable for immunohistochemical analysis of mouse tissues.
T5405 18838-18845 RB denotes However
T5406 18874-18877 VBD denotes was
T5407 18845-18847 , denotes ,
T5408 18847-18860 RB denotes unfortunately
T5409 18861-18864 DT denotes the
T5410 18865-18873 NN denotes antibody
T5411 18878-18881 RB denotes not
T5412 18882-18890 JJ denotes suitable
T5413 18891-18894 IN denotes for
T5414 18895-18914 JJ denotes immunohistochemical
T5415 18915-18923 NN denotes analysis
T5416 18924-18926 IN denotes of
T5417 18927-18932 NN denotes mouse
T5418 18933-18940 NNS denotes tissues
T5419 18940-18941 . denotes .
T5499 18943-18950 NNP denotes Western
T5500 18951-18955 NN denotes blot
T5501 18956-18964 NN denotes analysis
T5502 18964-19057 sentence denotes The absence of ADAM22 protein in the Adam22 -/- mice was confirmed by Western blot analysis.
T5503 18965-18968 DT denotes The
T5504 18969-18976 NN denotes absence
T5505 19022-19031 VBN denotes confirmed
T5506 18977-18979 IN denotes of
T5507 18980-18986 NN denotes ADAM22
T5508 18987-18994 NN denotes protein
T5509 18995-18997 IN denotes in
T5510 18998-19001 DT denotes the
T5511 19013-19017 NNS denotes mice
T5512 19002-19008 NN denotes Adam22
T5513 19009-19010 SYM denotes -
T5514 19010-19011 HYPH denotes /
T5515 19011-19012 SYM denotes -
T5516 19018-19021 VBD denotes was
T5517 19032-19034 IN denotes by
T5518 19035-19042 NNP denotes Western
T5519 19043-19047 NN denotes blot
T5520 19048-19056 NN denotes analysis
T5521 19056-19057 . denotes .
T5522 19057-19294 sentence denotes Briefly, the cerebellum was isolated from a P14.5 mouse of each genotype and homogenized with a Polytron homogenizer in cell lysis buffer (50 mM Tris-HCl, pH 7.5, 100 mM NaCl, 1% NP-40, Complete protease inhibitors [Roche Diagnostics]).
T5523 19058-19065 RB denotes Briefly
T5524 19086-19094 VBN denotes isolated
T5525 19065-19067 , denotes ,
T5526 19067-19070 DT denotes the
T5527 19071-19081 NN denotes cerebellum
T5528 19082-19085 VBD denotes was
T5529 19095-19099 IN denotes from
T5530 19100-19101 DT denotes a
T5531 19108-19113 NN denotes mouse
T5532 19102-19107 NN denotes P14.5
T5533 19114-19116 IN denotes of
T5534 19117-19121 DT denotes each
T5535 19122-19130 NN denotes genotype
T5536 19131-19134 CC denotes and
T5537 19135-19146 VBN denotes homogenized
T5538 19147-19151 IN denotes with
T5539 19152-19153 DT denotes a
T5540 19163-19174 NN denotes homogenizer
T5541 19154-19162 NN denotes Polytron
T5542 19175-19177 IN denotes in
T5543 19178-19182 NN denotes cell
T5544 19183-19188 NN denotes lysis
T5545 19189-19195 NN denotes buffer
T5546 19196-19197 -LRB- denotes (
T5547 19280-19291 NNP denotes Diagnostics
T5548 19197-19199 CD denotes 50
T5549 19200-19202 NN denotes mM
T5550 19208-19211 NN denotes HCl
T5551 19203-19207 NN denotes Tris
T5552 19207-19208 HYPH denotes -
T5553 19211-19213 , denotes ,
T5554 19213-19215 NN denotes pH
T5555 19216-19219 CD denotes 7.5
T5556 19219-19221 , denotes ,
T5557 19221-19224 CD denotes 100
T5558 19225-19227 NN denotes mM
T5559 19228-19232 NN denotes NaCl
T5560 19232-19234 , denotes ,
T5561 19234-19235 CD denotes 1
T5562 19235-19236 NN denotes %
T5563 19237-19239 NN denotes NP
T5564 19239-19240 HYPH denotes -
T5565 19240-19242 CD denotes 40
T5566 19242-19244 , denotes ,
T5567 19244-19252 JJ denotes Complete
T5568 19262-19272 NNS denotes inhibitors
T5569 19253-19261 NN denotes protease
T5570 19273-19274 -LRB- denotes [
T5571 19274-19279 NNP denotes Roche
T5572 19291-19292 -RRB- denotes ]
T5573 19292-19293 -RRB- denotes )
T5574 19293-19294 . denotes .
T5575 19294-19435 sentence denotes After removal of cell debris by centrifugation, the supernatant was separated on 10% SDS-PAGE, and transferred to a nitrocellulose membrane.
T5576 19295-19300 IN denotes After
T5577 19363-19372 VBN denotes separated
T5578 19301-19308 NN denotes removal
T5579 19309-19311 IN denotes of
T5580 19312-19316 NN denotes cell
T5581 19317-19323 NN denotes debris
T5582 19324-19326 IN denotes by
T5583 19327-19341 NN denotes centrifugation
T5584 19341-19343 , denotes ,
T5585 19343-19346 DT denotes the
T5586 19347-19358 NN denotes supernatant
T5587 19359-19362 VBD denotes was
T5588 19373-19375 IN denotes on
T5589 19376-19378 CD denotes 10
T5590 19378-19379 NN denotes %
T5591 19384-19388 NN denotes PAGE
T5592 19380-19383 NN denotes SDS
T5593 19383-19384 HYPH denotes -
T5594 19388-19390 , denotes ,
T5595 19390-19393 CC denotes and
T5596 19394-19405 VBN denotes transferred
T5597 19406-19408 IN denotes to
T5598 19409-19410 DT denotes a
T5599 19426-19434 NN denotes membrane
T5600 19411-19425 NN denotes nitrocellulose
T5601 19434-19435 . denotes .
T5602 19435-19543 sentence denotes The blot was then incubated with polyclonal antibody against the cytoplasmic domain of ADAM22 (at 1:1,000).
T5603 19436-19439 DT denotes The
T5604 19440-19444 NN denotes blot
T5605 19454-19463 VBN denotes incubated
T5606 19445-19448 VBD denotes was
T5607 19449-19453 RB denotes then
T5608 19464-19468 IN denotes with
T5609 19469-19479 JJ denotes polyclonal
T5610 19480-19488 NN denotes antibody
T5611 19489-19496 IN denotes against
T5612 19497-19500 DT denotes the
T5613 19513-19519 NN denotes domain
T5614 19501-19512 JJ denotes cytoplasmic
T5615 19520-19522 IN denotes of
T5616 19523-19529 NN denotes ADAM22
T5617 19530-19531 -LRB- denotes (
T5618 19531-19533 IN denotes at
T5619 19534-19535 CD denotes 1
T5620 19535-19536 SYM denotes :
T5621 19536-19541 CD denotes 1,000
T5622 19541-19542 -RRB- denotes )
T5623 19542-19543 . denotes .
T5624 19543-19709 sentence denotes Bound antibodies were visualized with horseradish peroxidase-labelled second antibody and a ECL-plus chemiluminescence detection system (Amersham Biosciences Corp.).
T5625 19544-19549 VBN denotes Bound
T5626 19550-19560 NNS denotes antibodies
T5627 19566-19576 VBN denotes visualized
T5628 19561-19565 VBD denotes were
T5629 19577-19581 IN denotes with
T5630 19582-19593 NN denotes horseradish
T5631 19621-19629 NN denotes antibody
T5632 19594-19604 NN denotes peroxidase
T5633 19605-19613 VBN denotes labelled
T5634 19604-19605 HYPH denotes -
T5635 19614-19620 JJ denotes second
T5636 19630-19633 CC denotes and
T5637 19634-19635 DT denotes a
T5638 19673-19679 NN denotes system
T5639 19636-19639 NN denotes ECL
T5640 19640-19644 NN denotes plus
T5641 19639-19640 HYPH denotes -
T5642 19645-19662 NN denotes chemiluminescence
T5643 19663-19672 NN denotes detection
T5644 19680-19681 -LRB- denotes (
T5645 19702-19707 NNP denotes Corp.
T5646 19681-19689 NNP denotes Amersham
T5647 19690-19701 NNP denotes Biosciences
T5648 19707-19708 -RRB- denotes )
T5649 19708-19709 . denotes .
T5703 19711-19718 JJ denotes Primary
T5704 19727-19731 NN denotes cell
T5705 19719-19726 NNP denotes Schwann
T5706 19732-19739 NN denotes culture
T5707 19739-19867 sentence denotes Primary Schwann cells were prepared from 4-month-old C57BL/6 mice according to Manent's protocol [32] with minor modifications.
T5708 19740-19747 JJ denotes Primary
T5709 19756-19761 NNS denotes cells
T5710 19748-19755 NNP denotes Schwann
T5711 19767-19775 VBN denotes prepared
T5712 19762-19766 VBD denotes were
T5713 19776-19780 IN denotes from
T5714 19781-19782 CD denotes 4
T5715 19783-19788 NN denotes month
T5716 19782-19783 HYPH denotes -
T5717 19789-19792 JJ denotes old
T5718 19788-19789 HYPH denotes -
T5719 19801-19805 NNS denotes mice
T5720 19793-19798 NN denotes C57BL
T5721 19798-19799 HYPH denotes /
T5722 19799-19800 CD denotes 6
T5723 19806-19815 VBG denotes according
T5724 19816-19818 IN denotes to
T5725 19819-19825 NNP denotes Manent
T5726 19828-19836 NN denotes protocol
T5727 19825-19827 POS denotes 's
T5728 19837-19838 -LRB- denotes [
T5729 19838-19840 CD denotes 32
T5730 19840-19841 -RRB- denotes ]
T5731 19842-19846 IN denotes with
T5732 19847-19852 JJ denotes minor
T5733 19853-19866 NNS denotes modifications
T5734 19866-19867 . denotes .
T5735 19867-20199 sentence denotes Briefly, sciatic nerves were removed and incubated for 7 days in the pre-treatment medium, which consisted of D-MEM (high glucose) supplemented with 10% FCS, 50 mg/ml gentamicin (Invitrogen Corp.), 2.5 μg/ml fungizone (Invitrogen Corp.), 2 μM forskolin (EMD Biosciences Inc.) and 10 ng/ml recombinant heregulin-beta1 (R&D Systems).
T5736 19868-19875 RB denotes Briefly
T5737 19897-19904 VBN denotes removed
T5738 19875-19877 , denotes ,
T5739 19877-19884 JJ denotes sciatic
T5740 19885-19891 NNS denotes nerves
T5741 19892-19896 VBD denotes were
T5742 19905-19908 CC denotes and
T5743 19909-19918 VBN denotes incubated
T5744 19919-19922 IN denotes for
T5745 19923-19924 CD denotes 7
T5746 19925-19929 NNS denotes days
T5747 19930-19932 IN denotes in
T5748 19933-19936 DT denotes the
T5749 19951-19957 NN denotes medium
T5750 19937-19950 JJ denotes pre-treatment
T5751 19957-19959 , denotes ,
T5752 19959-19964 WDT denotes which
T5753 19965-19974 VBD denotes consisted
T5754 19975-19977 IN denotes of
T5755 19978-19979 NN denotes D
T5756 19980-19983 NN denotes MEM
T5757 19979-19980 HYPH denotes -
T5758 19984-19985 -LRB- denotes (
T5759 19990-19997 NN denotes glucose
T5760 19985-19989 JJ denotes high
T5761 19997-19998 -RRB- denotes )
T5762 19999-20011 VBN denotes supplemented
T5763 20012-20016 IN denotes with
T5764 20017-20019 CD denotes 10
T5765 20019-20020 NN denotes %
T5766 20021-20024 NN denotes FCS
T5767 20024-20026 , denotes ,
T5768 20026-20028 CD denotes 50
T5769 20029-20031 NN denotes mg
T5770 20035-20045 NN denotes gentamicin
T5771 20031-20032 SYM denotes /
T5772 20032-20034 NN denotes ml
T5773 20046-20047 -LRB- denotes (
T5774 20058-20063 NNP denotes Corp.
T5775 20047-20057 NNP denotes Invitrogen
T5776 20063-20064 -RRB- denotes )
T5777 20064-20066 , denotes ,
T5778 20066-20069 CD denotes 2.5
T5779 20070-20072 NN denotes μg
T5780 20076-20085 NN denotes fungizone
T5781 20072-20073 SYM denotes /
T5782 20073-20075 NN denotes ml
T5783 20086-20087 -LRB- denotes (
T5784 20098-20103 NNP denotes Corp.
T5785 20087-20097 NNP denotes Invitrogen
T5786 20103-20104 -RRB- denotes )
T5787 20104-20106 , denotes ,
T5788 20106-20107 CD denotes 2
T5789 20108-20110 NN denotes μM
T5790 20111-20120 NN denotes forskolin
T5791 20121-20122 -LRB- denotes (
T5792 20138-20142 NNP denotes Inc.
T5793 20122-20125 NNP denotes EMD
T5794 20126-20137 NNP denotes Biosciences
T5795 20142-20143 -RRB- denotes )
T5796 20144-20147 CC denotes and
T5797 20148-20150 CD denotes 10
T5798 20151-20153 NN denotes ng
T5799 20179-20184 NN denotes beta1
T5800 20153-20154 SYM denotes /
T5801 20154-20156 NN denotes ml
T5802 20157-20168 JJ denotes recombinant
T5803 20169-20178 NN denotes heregulin
T5804 20178-20179 HYPH denotes -
T5805 20185-20186 -LRB- denotes (
T5806 20190-20197 NNP denotes Systems
T5807 20186-20187 NNP denotes R
T5808 20187-20188 CC denotes &
T5809 20188-20189 NNP denotes D
T5810 20197-20198 -RRB- denotes )
T5811 20198-20199 . denotes .
T5812 20199-20437 sentence denotes For dissociation, cultured sciatic nerves were cut into pieces and incubated at 37°C for 3 hours in Opti-MEM medium (Invitrogen Corp.) containing 130 U/ml collagenase type I (Invitrogen Corp.) and 0.4 U/ml dispase II (Roche Diagnostics).
T5813 20200-20203 IN denotes For
T5814 20247-20250 VBN denotes cut
T5815 20204-20216 NN denotes dissociation
T5816 20216-20218 , denotes ,
T5817 20218-20226 VBN denotes cultured
T5818 20235-20241 NNS denotes nerves
T5819 20227-20234 JJ denotes sciatic
T5820 20242-20246 VBD denotes were
T5821 20251-20255 IN denotes into
T5822 20256-20262 NNS denotes pieces
T5823 20263-20266 CC denotes and
T5824 20267-20276 VBN denotes incubated
T5825 20277-20279 IN denotes at
T5826 20280-20282 CD denotes 37
T5827 20282-20284 NN denotes °C
T5828 20285-20288 IN denotes for
T5829 20289-20290 CD denotes 3
T5830 20291-20296 NNS denotes hours
T5831 20297-20299 IN denotes in
T5832 20300-20304 NN denotes Opti
T5833 20305-20308 NN denotes MEM
T5834 20304-20305 HYPH denotes -
T5835 20309-20315 NN denotes medium
T5836 20316-20317 -LRB- denotes (
T5837 20328-20333 NNP denotes Corp.
T5838 20317-20327 NNP denotes Invitrogen
T5839 20333-20334 -RRB- denotes )
T5840 20335-20345 VBG denotes containing
T5841 20346-20349 CD denotes 130
T5842 20350-20351 NN denotes U
T5843 20367-20371 NN denotes type
T5844 20351-20352 SYM denotes /
T5845 20352-20354 NN denotes ml
T5846 20355-20366 NN denotes collagenase
T5847 20372-20373 CD denotes I
T5848 20374-20375 -LRB- denotes (
T5849 20386-20391 NNP denotes Corp.
T5850 20375-20385 NNP denotes Invitrogen
T5851 20391-20392 -RRB- denotes )
T5852 20393-20396 CC denotes and
T5853 20397-20400 CD denotes 0.4
T5854 20401-20402 NN denotes U
T5855 20406-20413 NN denotes dispase
T5856 20402-20403 SYM denotes /
T5857 20403-20405 NN denotes ml
T5858 20414-20416 CD denotes II
T5859 20417-20418 -LRB- denotes (
T5860 20424-20435 NNP denotes Diagnostics
T5861 20418-20423 NNP denotes Roche
T5862 20435-20436 -RRB- denotes )
T5863 20436-20437 . denotes .
T5864 20437-20567 sentence denotes Dissociated cells were resuspended in the pre-treatment medium and plated on Poly-D-Lysine/Laminin coated plate (BD Biosciences).
T5865 20438-20449 VBN denotes Dissociated
T5866 20450-20455 NNS denotes cells
T5867 20461-20472 VBN denotes resuspended
T5868 20456-20460 VBD denotes were
T5869 20473-20475 IN denotes in
T5870 20476-20479 DT denotes the
T5871 20494-20500 NN denotes medium
T5872 20480-20493 JJ denotes pre-treatment
T5873 20501-20504 CC denotes and
T5874 20505-20511 VBN denotes plated
T5875 20512-20514 IN denotes on
T5876 20515-20519 NN denotes Poly
T5877 20522-20528 NN denotes Lysine
T5878 20519-20520 HYPH denotes -
T5879 20520-20521 NN denotes D
T5880 20521-20522 HYPH denotes -
T5881 20537-20543 VBN denotes coated
T5882 20528-20529 HYPH denotes /
T5883 20529-20536 NN denotes Laminin
T5884 20544-20549 NN denotes plate
T5885 20550-20551 -LRB- denotes (
T5886 20554-20565 NNS denotes Biosciences
T5887 20551-20553 NN denotes BD
T5888 20565-20566 -RRB- denotes )
T5889 20566-20567 . denotes .
T5890 20567-20681 sentence denotes The purity of the cultured Schwann cells, as determined by indirect immunofluorescence analysis, approached 90 %.
T5891 20568-20571 DT denotes The
T5892 20572-20578 NN denotes purity
T5893 20665-20675 VBD denotes approached
T5894 20579-20581 IN denotes of
T5895 20582-20585 DT denotes the
T5896 20603-20608 NNS denotes cells
T5897 20586-20594 VBN denotes cultured
T5898 20595-20602 NNP denotes Schwann
T5899 20608-20610 , denotes ,
T5900 20610-20612 IN denotes as
T5901 20613-20623 VBN denotes determined
T5902 20624-20626 IN denotes by
T5903 20627-20635 JJ denotes indirect
T5904 20655-20663 NN denotes analysis
T5905 20636-20654 NN denotes immunofluorescence
T5906 20663-20665 , denotes ,
T5907 20676-20678 CD denotes 90
T5908 20679-20680 NN denotes %
T5909 20680-20681 . denotes .
T5980 20683-20685 NN denotes RT
T5981 20686-20689 NN denotes PCR
T5982 20685-20686 HYPH denotes -
T5983 20690-20698 NN denotes analysis
T5984 20698-20747 sentence denotes Adult C57BL/6 male mice were used in this study.
T5985 20699-20704 JJ denotes Adult
T5986 20718-20722 NNS denotes mice
T5987 20705-20710 NN denotes C57BL
T5988 20710-20711 HYPH denotes /
T5989 20711-20712 CD denotes 6
T5990 20713-20717 JJ denotes male
T5991 20728-20732 VBN denotes used
T5992 20723-20727 VBD denotes were
T5993 20733-20735 IN denotes in
T5994 20736-20740 DT denotes this
T5995 20741-20746 NN denotes study
T5996 20746-20747 . denotes .
T5997 20747-21150 sentence denotes Total RNAs purified from the cerebellum, spinal cord, sciatic nerve, DRG and cultured Schwann cells using TRIzol (Invitrogen Corp.) and RNeasy kit (Qiagen GmbH) were analysed by RT-PCR using SuperScript II and random primer (Invitrogen Corp.), and PCR amplification (40 cycles; 94°C-30 s, 60°C-30 s and 68°C-5 min) with Expand Hi-Fidelity DNA polymerase (Roche Diagnostics) and ADAM22-specific primers.
T5998 20748-20753 JJ denotes Total
T5999 20754-20758 NNS denotes RNAs
T6000 20914-20922 VBN denotes analysed
T6001 20759-20767 VBN denotes purified
T6002 20768-20772 IN denotes from
T6003 20773-20776 DT denotes the
T6004 20777-20787 NN denotes cerebellum
T6005 20787-20789 , denotes ,
T6006 20789-20795 JJ denotes spinal
T6007 20796-20800 NN denotes cord
T6008 20800-20802 , denotes ,
T6009 20802-20809 JJ denotes sciatic
T6010 20810-20815 NN denotes nerve
T6011 20815-20817 , denotes ,
T6012 20817-20820 NN denotes DRG
T6013 20821-20824 CC denotes and
T6014 20825-20833 VBN denotes cultured
T6015 20842-20847 NNS denotes cells
T6016 20834-20841 NNP denotes Schwann
T6017 20848-20853 VBG denotes using
T6018 20854-20860 NN denotes TRIzol
T6019 20861-20862 -LRB- denotes (
T6020 20873-20878 NNP denotes Corp.
T6021 20862-20872 NNP denotes Invitrogen
T6022 20878-20879 -RRB- denotes )
T6023 20880-20883 CC denotes and
T6024 20884-20890 NN denotes RNeasy
T6025 20891-20894 NN denotes kit
T6026 20895-20896 -LRB- denotes (
T6027 20903-20907 NNP denotes GmbH
T6028 20896-20902 NNP denotes Qiagen
T6029 20907-20908 -RRB- denotes )
T6030 20909-20913 VBD denotes were
T6031 20923-20925 IN denotes by
T6032 20926-20928 NN denotes RT
T6033 20929-20932 NN denotes PCR
T6034 20928-20929 HYPH denotes -
T6035 20933-20938 VBG denotes using
T6036 20939-20950 NNP denotes SuperScript
T6037 20951-20953 CD denotes II
T6038 20954-20957 CC denotes and
T6039 20958-20964 JJ denotes random
T6040 20965-20971 NN denotes primer
T6041 20972-20973 -LRB- denotes (
T6042 20984-20989 NNP denotes Corp.
T6043 20973-20983 NNP denotes Invitrogen
T6044 20989-20990 -RRB- denotes )
T6045 20990-20992 , denotes ,
T6046 20992-20995 CC denotes and
T6047 20996-20999 NN denotes PCR
T6048 21000-21013 NN denotes amplification
T6049 21014-21015 -LRB- denotes (
T6050 21028-21030 NN denotes °C
T6051 21015-21017 CD denotes 40
T6052 21018-21024 NNS denotes cycles
T6053 21024-21025 : denotes ;
T6054 21026-21028 CD denotes 94
T6055 21030-21031 HYPH denotes -
T6056 21031-21033 CD denotes 30
T6057 21034-21035 NN denotes s
T6058 21035-21037 , denotes ,
T6059 21037-21039 CD denotes 60
T6060 21039-21041 NN denotes °C
T6061 21041-21042 HYPH denotes -
T6062 21042-21044 CD denotes 30
T6063 21045-21046 NN denotes s
T6064 21047-21050 CC denotes and
T6065 21051-21053 CD denotes 68
T6066 21053-21055 NN denotes °C
T6067 21055-21056 HYPH denotes -
T6068 21056-21057 CD denotes 5
T6069 21058-21061 NN denotes min
T6070 21061-21062 -RRB- denotes )
T6071 21063-21067 IN denotes with
T6072 21068-21074 NNP denotes Expand
T6073 21091-21101 NN denotes polymerase
T6074 21075-21077 NNP denotes Hi
T6075 21078-21086 NNP denotes Fidelity
T6076 21077-21078 HYPH denotes -
T6077 21087-21090 NN denotes DNA
T6078 21102-21103 -LRB- denotes (
T6079 21109-21120 NNP denotes Diagnostics
T6080 21103-21108 NNP denotes Roche
T6081 21120-21121 -RRB- denotes )
T6082 21122-21125 CC denotes and
T6083 21126-21132 NN denotes ADAM22
T6084 21133-21141 JJ denotes specific
T6085 21132-21133 HYPH denotes -
T6086 21142-21149 NNS denotes primers
T6087 21149-21150 . denotes .
T6088 21150-21329 sentence denotes To detect splicing variants in the cytoplasmic domain, a forward primer was placed just upstream of the transmembrane domain and a reverse primer was set on the terminating exon.
T6089 21151-21153 TO denotes To
T6090 21154-21160 VB denotes detect
T6091 21227-21233 VBN denotes placed
T6092 21161-21169 NN denotes splicing
T6093 21170-21178 NNS denotes variants
T6094 21179-21181 IN denotes in
T6095 21182-21185 DT denotes the
T6096 21198-21204 NN denotes domain
T6097 21186-21197 JJ denotes cytoplasmic
T6098 21204-21206 , denotes ,
T6099 21206-21207 DT denotes a
T6100 21216-21222 NN denotes primer
T6101 21208-21215 JJ denotes forward
T6102 21223-21226 VBD denotes was
T6103 21234-21238 RB denotes just
T6104 21239-21247 RB denotes upstream
T6105 21248-21250 IN denotes of
T6106 21251-21254 DT denotes the
T6107 21269-21275 NN denotes domain
T6108 21255-21268 JJ denotes transmembrane
T6109 21276-21279 CC denotes and
T6110 21280-21281 DT denotes a
T6111 21290-21296 NN denotes primer
T6112 21282-21289 JJ denotes reverse
T6113 21301-21304 VBN denotes set
T6114 21297-21300 VBD denotes was
T6115 21305-21307 IN denotes on
T6116 21308-21311 DT denotes the
T6117 21324-21328 NN denotes exon
T6118 21312-21323 VBG denotes terminating
T6119 21328-21329 . denotes .
T6120 21329-21377 sentence denotes The primers used in this study were as follows.
T6121 21330-21333 DT denotes The
T6122 21334-21341 NNS denotes primers
T6123 21361-21365 VBD denotes were
T6124 21342-21346 VBN denotes used
T6125 21347-21349 IN denotes in
T6126 21350-21354 DT denotes this
T6127 21355-21360 NN denotes study
T6128 21366-21368 IN denotes as
T6129 21369-21376 VBZ denotes follows
T6130 21376-21377 . denotes .
T6131 21377-21474 sentence denotes ISH04-forward: 5'-AACAGGCACTGGACAGGGGCTGAC-3' and ISH04-reverse: 5'-AATGGATGTCTCCCATAGCCTGGC-3'.
T6132 21378-21383 NN denotes ISH04
T6133 21383-21384 HYPH denotes -
T6134 21384-21391 JJ denotes forward
T6135 21391-21393 : denotes :
T6136 21393-21394 CD denotes 5
T6137 21396-21420 NN denotes AACAGGCACTGGACAGGGGCTGAC
T6138 21394-21395 SYM denotes '
T6139 21395-21396 HYPH denotes -
T6140 21420-21421 HYPH denotes -
T6141 21421-21422 CD denotes 3
T6142 21422-21423 SYM denotes '
T6143 21424-21427 CC denotes and
T6144 21428-21433 NN denotes ISH04
T6145 21433-21434 HYPH denotes -
T6146 21434-21441 JJ denotes reverse
T6147 21441-21443 : denotes :
T6148 21443-21444 CD denotes 5
T6149 21446-21470 NN denotes AATGGATGTCTCCCATAGCCTGGC
T6150 21444-21445 SYM denotes '
T6151 21445-21446 HYPH denotes -
T6152 21470-21471 HYPH denotes -
T6153 21471-21472 CD denotes 3
T6154 21472-21473 SYM denotes '
T6155 21473-21474 . denotes .
T6212 21476-21490 NN denotes Histopathology
T6213 21490-21531 sentence denotes Mice were anesthetized with ethyl ether.
T6214 21491-21495 NNS denotes Mice
T6215 21501-21513 VBN denotes anesthetized
T6216 21496-21500 VBD denotes were
T6217 21514-21518 IN denotes with
T6218 21519-21524 NN denotes ethyl
T6219 21525-21530 NN denotes ether
T6220 21530-21531 . denotes .
T6221 21531-21650 sentence denotes Whole-body perfusion by 2% paraformaldehyde-glutaraldehyde solution followed by heparin-included saline was performed.
T6222 21532-21537 JJ denotes Whole
T6223 21538-21542 NN denotes body
T6224 21537-21538 HYPH denotes -
T6225 21543-21552 NN denotes perfusion
T6226 21640-21649 VBN denotes performed
T6227 21553-21555 IN denotes by
T6228 21556-21557 CD denotes 2
T6229 21557-21558 NN denotes %
T6230 21591-21599 NN denotes solution
T6231 21559-21575 NN denotes paraformaldehyde
T6232 21576-21590 NN denotes glutaraldehyde
T6233 21575-21576 HYPH denotes -
T6234 21600-21608 VBN denotes followed
T6235 21609-21611 IN denotes by
T6236 21612-21619 NN denotes heparin
T6237 21620-21628 VBN denotes included
T6238 21619-21620 HYPH denotes -
T6239 21629-21635 NN denotes saline
T6240 21636-21639 VBD denotes was
T6241 21649-21650 . denotes .
T6242 21650-21764 sentence denotes Sciatic nerves, trigeminal nerves, brain and spinal cord were removed and fixed in 10% neutral-buffered formalin.
T6243 21651-21658 JJ denotes Sciatic
T6244 21659-21665 NNS denotes nerves
T6245 21713-21720 VBN denotes removed
T6246 21665-21667 , denotes ,
T6247 21667-21677 JJ denotes trigeminal
T6248 21678-21684 NNS denotes nerves
T6249 21684-21686 , denotes ,
T6250 21686-21691 NN denotes brain
T6251 21692-21695 CC denotes and
T6252 21696-21702 JJ denotes spinal
T6253 21703-21707 NN denotes cord
T6254 21708-21712 VBD denotes were
T6255 21721-21724 CC denotes and
T6256 21725-21730 VBN denotes fixed
T6257 21731-21733 IN denotes in
T6258 21734-21736 CD denotes 10
T6259 21736-21737 NN denotes %
T6260 21755-21763 NN denotes formalin
T6261 21738-21745 JJ denotes neutral
T6262 21746-21754 VBN denotes buffered
T6263 21745-21746 HYPH denotes -
T6264 21763-21764 . denotes .
T6265 21764-21912 sentence denotes The spinal cord and other nerve blocks were washed and postfixed with 2% osmium tetroxide, and were dehydrated in ethanol and equilibrated in Epon.
T6266 21765-21768 DT denotes The
T6267 21776-21780 NN denotes cord
T6268 21769-21775 JJ denotes spinal
T6269 21809-21815 VBN denotes washed
T6270 21781-21784 CC denotes and
T6271 21785-21790 JJ denotes other
T6272 21797-21803 NNS denotes blocks
T6273 21791-21796 NN denotes nerve
T6274 21804-21808 VBD denotes were
T6275 21816-21819 CC denotes and
T6276 21820-21829 VBN denotes postfixed
T6277 21830-21834 IN denotes with
T6278 21835-21836 CD denotes 2
T6279 21836-21837 NN denotes %
T6280 21845-21854 NN denotes tetroxide
T6281 21838-21844 NN denotes osmium
T6282 21854-21856 , denotes ,
T6283 21856-21859 CC denotes and
T6284 21860-21864 VBD denotes were
T6285 21865-21875 VBN denotes dehydrated
T6286 21876-21878 IN denotes in
T6287 21879-21886 NN denotes ethanol
T6288 21887-21890 CC denotes and
T6289 21891-21903 VBN denotes equilibrated
T6290 21904-21906 IN denotes in
T6291 21907-21911 NN denotes Epon
T6292 21911-21912 . denotes .
T6293 21912-22030 sentence denotes Epon embedded semithin sections were stained with toluidine blue and were subjected to light microscopic examination.
T6294 21913-21917 NN denotes Epon
T6295 21918-21926 VBN denotes embedded
T6296 21936-21944 NNS denotes sections
T6297 21927-21935 JJ denotes semithin
T6298 21950-21957 VBN denotes stained
T6299 21945-21949 VBD denotes were
T6300 21958-21962 IN denotes with
T6301 21963-21972 NN denotes toluidine
T6302 21973-21977 NN denotes blue
T6303 21978-21981 CC denotes and
T6304 21982-21986 VBD denotes were
T6305 21987-21996 VBN denotes subjected
T6306 21997-21999 IN denotes to
T6307 22000-22005 NN denotes light
T6308 22006-22017 JJ denotes microscopic
T6309 22018-22029 NN denotes examination
T6310 22029-22030 . denotes .
T6311 22030-22223 sentence denotes For electron microscopic analysis, thin sections were cut using an ultramicrotome, stained with 1.5% uranylacetate in 50% ethanol and 0.8% lead citrate, and analysed using electron microscope.
T6312 22031-22034 IN denotes For
T6313 22085-22088 VBN denotes cut
T6314 22035-22043 NN denotes electron
T6315 22044-22055 JJ denotes microscopic
T6316 22056-22064 NN denotes analysis
T6317 22064-22066 , denotes ,
T6318 22066-22070 JJ denotes thin
T6319 22071-22079 NNS denotes sections
T6320 22080-22084 VBD denotes were
T6321 22089-22094 VBG denotes using
T6322 22095-22097 DT denotes an
T6323 22098-22112 NN denotes ultramicrotome
T6324 22112-22114 , denotes ,
T6325 22114-22121 VBN denotes stained
T6326 22122-22126 IN denotes with
T6327 22127-22130 CD denotes 1.5
T6328 22130-22131 NN denotes %
T6329 22132-22145 NN denotes uranylacetate
T6330 22146-22148 IN denotes in
T6331 22149-22151 CD denotes 50
T6332 22151-22152 NN denotes %
T6333 22153-22160 NN denotes ethanol
T6334 22161-22164 CC denotes and
T6335 22165-22168 CD denotes 0.8
T6336 22168-22169 NN denotes %
T6337 22175-22182 NN denotes citrate
T6338 22170-22174 NN denotes lead
T6339 22182-22184 , denotes ,
T6340 22184-22187 CC denotes and
T6341 22188-22196 VBN denotes analysed
T6342 22197-22202 VBG denotes using
T6343 22203-22211 NN denotes electron
T6344 22212-22222 NN denotes microscope
T6345 22222-22223 . denotes .
T6346 22223-22312 sentence denotes All procedures were conducted according to the Eisai Animal Care Committee's guidelines.
T6347 22224-22227 DT denotes All
T6348 22228-22238 NNS denotes procedures
T6349 22244-22253 VBN denotes conducted
T6350 22239-22243 VBD denotes were
T6351 22254-22263 VBG denotes according
T6352 22264-22266 IN denotes to
T6353 22267-22270 DT denotes the
T6354 22289-22298 NNP denotes Committee
T6355 22271-22276 NNP denotes Eisai
T6356 22277-22283 NNP denotes Animal
T6357 22284-22288 NNP denotes Care
T6358 22301-22311 NNS denotes guidelines
T6359 22298-22300 POS denotes 's
T6360 22311-22312 . denotes .
T6425 22314-22334 NN denotes Immunohistochemistry
T6426 22334-22471 sentence denotes Frozen sections were rinsed in 0.1% Triton X-100/PBS at room temperature for 1 hour, and were blocked in BLOCKACE solution (Dai-nippon).
T6427 22335-22341 JJ denotes Frozen
T6428 22342-22350 NNS denotes sections
T6429 22356-22362 VBN denotes rinsed
T6430 22351-22355 VBD denotes were
T6431 22363-22365 IN denotes in
T6432 22366-22369 CD denotes 0.1
T6433 22369-22370 NN denotes %
T6434 22371-22377 NN denotes Triton
T6435 22378-22379 NN denotes X
T6436 22379-22380 HYPH denotes -
T6437 22380-22383 CD denotes 100
T6438 22383-22384 HYPH denotes /
T6439 22384-22387 NN denotes PBS
T6440 22388-22390 IN denotes at
T6441 22391-22395 NN denotes room
T6442 22396-22407 NN denotes temperature
T6443 22408-22411 IN denotes for
T6444 22412-22413 CD denotes 1
T6445 22414-22418 NN denotes hour
T6446 22418-22420 , denotes ,
T6447 22420-22423 CC denotes and
T6448 22424-22428 VBD denotes were
T6449 22429-22436 VBN denotes blocked
T6450 22437-22439 IN denotes in
T6451 22440-22448 NN denotes BLOCKACE
T6452 22449-22457 NN denotes solution
T6453 22458-22459 -LRB- denotes (
T6454 22463-22469 NNP denotes nippon
T6455 22459-22462 NNP denotes Dai
T6456 22462-22463 HYPH denotes -
T6457 22469-22470 -RRB- denotes )
T6458 22470-22471 . denotes .
T6459 22471-22546 sentence denotes The following antibodies were incubated overnight in 0.1% BLOCKACE at 4°C:
T6460 22472-22475 DT denotes The
T6461 22486-22496 NNS denotes antibodies
T6462 22476-22485 VBG denotes following
T6463 22502-22511 VBN denotes incubated
T6464 22497-22501 VBD denotes were
T6465 22512-22521 RB denotes overnight
T6466 22522-22524 IN denotes in
T6467 22525-22528 CD denotes 0.1
T6468 22528-22529 NN denotes %
T6469 22530-22538 NN denotes BLOCKACE
T6470 22539-22541 IN denotes at
T6471 22542-22543 CD denotes 4
T6472 22543-22545 NN denotes °C
T6473 22545-22546 : denotes :
T6474 22546-22761 sentence denotes monoclonal mouse anti-calbindin 28 K (Sigma, 1:200); monoclonal mouse anti-MBP (SMI-99, Sternberger Monoclonals Inc., 1:50); monoclonal mouse anti-Neu N (CHEMICON, 1:100); rabbit anti-S100 polyclonal (DAKO, 1:200).
T6475 22547-22557 JJ denotes monoclonal
T6476 22582-22583 NN denotes K
T6477 22558-22563 NN denotes mouse
T6478 22564-22578 JJ denotes anti-calbindin
T6479 22579-22581 CD denotes 28
T6480 22584-22585 -LRB- denotes (
T6481 22594-22597 CD denotes 200
T6482 22585-22590 NNP denotes Sigma
T6483 22590-22592 , denotes ,
T6484 22592-22593 CD denotes 1
T6485 22593-22594 : denotes :
T6486 22597-22598 -RRB- denotes )
T6487 22598-22599 : denotes ;
T6488 22600-22610 JJ denotes monoclonal
T6489 22611-22616 NN denotes mouse
T6490 22617-22625 JJ denotes anti-MBP
T6491 22626-22627 -LRB- denotes (
T6492 22667-22669 CD denotes 50
T6493 22627-22630 NNP denotes SMI
T6494 22630-22631 HYPH denotes -
T6495 22631-22633 CD denotes 99
T6496 22633-22635 , denotes ,
T6497 22635-22646 NNP denotes Sternberger
T6498 22647-22658 NNP denotes Monoclonals
T6499 22659-22663 NNP denotes Inc.
T6500 22663-22665 , denotes ,
T6501 22665-22666 CD denotes 1
T6502 22666-22667 SYM denotes :
T6503 22669-22670 -RRB- denotes )
T6504 22670-22671 : denotes ;
T6505 22672-22682 JJ denotes monoclonal
T6506 22698-22699 NN denotes N
T6507 22683-22688 NN denotes mouse
T6508 22689-22697 JJ denotes anti-Neu
T6509 22700-22701 -LRB- denotes (
T6510 22713-22716 CD denotes 100
T6511 22701-22709 NNP denotes CHEMICON
T6512 22709-22711 , denotes ,
T6513 22711-22712 CD denotes 1
T6514 22712-22713 SYM denotes :
T6515 22716-22717 -RRB- denotes )
T6516 22717-22718 : denotes ;
T6517 22719-22725 NN denotes rabbit
T6518 22726-22735 JJ denotes anti-S100
T6519 22736-22746 JJ denotes polyclonal
T6520 22747-22748 -LRB- denotes (
T6521 22756-22759 CD denotes 200
T6522 22748-22752 NNP denotes DAKO
T6523 22752-22754 , denotes ,
T6524 22754-22755 CD denotes 1
T6525 22755-22756 SYM denotes :
T6526 22759-22760 -RRB- denotes )
T6527 22760-22761 . denotes .
T6528 22761-22815 sentence denotes Nuclei were counterstained with 1 μg/ml DAPI (Sigma).
T6529 22762-22768 NNS denotes Nuclei
T6530 22774-22788 VBN denotes counterstained
T6531 22769-22773 VBD denotes were
T6532 22789-22793 IN denotes with
T6533 22794-22795 CD denotes 1
T6534 22796-22798 NN denotes μg
T6535 22802-22806 NN denotes DAPI
T6536 22798-22799 SYM denotes /
T6537 22799-22801 NN denotes ml
T6538 22807-22808 -LRB- denotes (
T6539 22808-22813 NNP denotes Sigma
T6540 22813-22814 -RRB- denotes )
T6541 22814-22815 . denotes .
T6542 22815-22912 sentence denotes MBP staining was performed after microwave epitope retrieval in 10 mmol/L citrate buffer (pH 6).
T6543 22816-22819 NN denotes MBP
T6544 22820-22828 NN denotes staining
T6545 22833-22842 VBN denotes performed
T6546 22829-22832 VBD denotes was
T6547 22843-22848 IN denotes after
T6548 22849-22858 NN denotes microwave
T6549 22867-22876 NN denotes retrieval
T6550 22859-22866 NN denotes epitope
T6551 22877-22879 IN denotes in
T6552 22880-22882 CD denotes 10
T6553 22883-22887 NN denotes mmol
T6554 22898-22904 NN denotes buffer
T6555 22887-22888 SYM denotes /
T6556 22888-22889 NN denotes L
T6557 22890-22897 NN denotes citrate
T6558 22905-22906 -LRB- denotes (
T6559 22906-22908 NN denotes pH
T6560 22909-22910 CD denotes 6
T6561 22910-22911 -RRB- denotes )
T6562 22911-22912 . denotes .
T6563 22912-23113 sentence denotes Sections were incubated for 1 hour with secondary antibodies: Cy3-labeled donkey anti-rabbit IgG (Jackson Immuno Research, 1:200), FITC-labelled donkey anti-mouse IgG (Jackson Immuno Research, 1:200).
T6564 22913-22921 NNS denotes Sections
T6565 22927-22936 VBN denotes incubated
T6566 22922-22926 VBD denotes were
T6567 22937-22940 IN denotes for
T6568 22941-22942 CD denotes 1
T6569 22943-22947 NN denotes hour
T6570 22948-22952 IN denotes with
T6571 22953-22962 JJ denotes secondary
T6572 22963-22973 NNS denotes antibodies
T6573 22973-22975 : denotes :
T6574 22975-22978 NN denotes Cy3
T6575 22979-22986 VBN denotes labeled
T6576 22978-22979 HYPH denotes -
T6577 23006-23009 NN denotes IgG
T6578 22987-22993 NN denotes donkey
T6579 22994-23005 JJ denotes anti-rabbit
T6580 23010-23011 -LRB- denotes (
T6581 23038-23041 CD denotes 200
T6582 23011-23018 NNP denotes Jackson
T6583 23026-23034 NNP denotes Research
T6584 23019-23025 NNP denotes Immuno
T6585 23034-23036 , denotes ,
T6586 23036-23037 CD denotes 1
T6587 23037-23038 SYM denotes :
T6588 23041-23042 -RRB- denotes )
T6589 23042-23044 , denotes ,
T6590 23044-23048 NN denotes FITC
T6591 23049-23057 VBN denotes labelled
T6592 23048-23049 HYPH denotes -
T6593 23076-23079 NN denotes IgG
T6594 23058-23064 NN denotes donkey
T6595 23065-23075 JJ denotes anti-mouse
T6596 23080-23081 -LRB- denotes (
T6597 23108-23111 CD denotes 200
T6598 23081-23088 NNP denotes Jackson
T6599 23096-23104 NNP denotes Research
T6600 23089-23095 NNP denotes Immuno
T6601 23104-23106 , denotes ,
T6602 23106-23107 CD denotes 1
T6603 23107-23108 SYM denotes :
T6604 23111-23112 -RRB- denotes )
T6605 23112-23113 . denotes .
T6606 23113-23226 sentence denotes Sections were photographed with an Olympus microscope (Olympus IX71) equipped with a high-resolution CCD camera.
T6607 23114-23122 NNS denotes Sections
T6608 23128-23140 VBN denotes photographed
T6609 23123-23127 VBD denotes were
T6610 23141-23145 IN denotes with
T6611 23146-23148 DT denotes an
T6612 23157-23167 NN denotes microscope
T6613 23149-23156 NNP denotes Olympus
T6614 23168-23169 -LRB- denotes (
T6615 23177-23181 NN denotes IX71
T6616 23169-23176 NNP denotes Olympus
T6617 23181-23182 -RRB- denotes )
T6618 23183-23191 VBN denotes equipped
T6619 23192-23196 IN denotes with
T6620 23197-23198 DT denotes a
T6621 23219-23225 NN denotes camera
T6622 23199-23203 JJ denotes high
T6623 23204-23214 NN denotes resolution
T6624 23203-23204 HYPH denotes -
T6625 23215-23218 NN denotes CCD
T6626 23225-23226 . denotes .
T6757 23520-23522 IN denotes as
T6692 23228-23231 NN denotes RNA
T6693 23240-23253 NN denotes hybridisation
T6694 23232-23234 FW denotes in
T6695 23235-23239 FW denotes situ
T6696 23253-23342 sentence denotes In situ hybridisation on frozen sections were carried out using 35S-labelled RNA probes.
T6697 23254-23256 FW denotes In
T6698 23257-23261 FW denotes situ
T6699 23262-23275 NN denotes hybridisation
T6700 23300-23307 VBN denotes carried
T6701 23276-23278 IN denotes on
T6702 23279-23285 VBN denotes frozen
T6703 23286-23294 NNS denotes sections
T6704 23295-23299 VBD denotes were
T6705 23308-23311 RP denotes out
T6706 23312-23317 VBG denotes using
T6707 23318-23321 CD denotes 35S
T6708 23322-23330 VBN denotes labelled
T6709 23321-23322 HYPH denotes -
T6710 23335-23341 NNS denotes probes
T6711 23331-23334 NN denotes RNA
T6712 23341-23342 . denotes .
T6713 23342-23498 sentence denotes Briefly, 610 bp of mouse ADAM22 cDNA (position: 1351–1960 from initiating ATG) was cloned into the pBluescript II SK(+) or the pBluescript II KS(+) vector.
T6714 23343-23350 RB denotes Briefly
T6715 23426-23432 VBN denotes cloned
T6716 23350-23352 , denotes ,
T6717 23352-23355 CD denotes 610
T6718 23356-23358 NN denotes bp
T6719 23359-23361 IN denotes of
T6720 23362-23367 NN denotes mouse
T6721 23375-23379 NN denotes cDNA
T6722 23368-23374 NN denotes ADAM22
T6723 23380-23381 -LRB- denotes (
T6724 23396-23400 CD denotes 1960
T6725 23381-23389 NN denotes position
T6726 23389-23391 : denotes :
T6727 23391-23395 CD denotes 1351
T6728 23395-23396 SYM denotes –
T6729 23401-23405 IN denotes from
T6730 23406-23416 VBG denotes initiating
T6731 23417-23420 NN denotes ATG
T6732 23420-23421 -RRB- denotes )
T6733 23422-23425 VBD denotes was
T6734 23433-23437 IN denotes into
T6735 23438-23441 DT denotes the
T6736 23457-23459 NN denotes SK
T6737 23442-23453 NNP denotes pBluescript
T6738 23454-23456 CD denotes II
T6739 23459-23460 -LRB- denotes (
T6740 23460-23461 SYM denotes +
T6741 23461-23462 -RRB- denotes )
T6742 23463-23465 CC denotes or
T6743 23466-23469 DT denotes the
T6744 23485-23487 NN denotes KS
T6745 23470-23481 NNP denotes pBluescript
T6746 23482-23484 CD denotes II
T6747 23491-23497 NN denotes vector
T6748 23487-23488 -LRB- denotes (
T6749 23488-23489 SYM denotes +
T6750 23489-23490 -RRB- denotes )
T6751 23497-23498 . denotes .
T6752 23498-23624 sentence denotes Using these plasmids as templates, sense and antisense labelled RNA probes were generated by T7 RNA polymerase and [α35S]UTP.
T6753 23499-23504 VBG denotes Using
T6754 23579-23588 VBN denotes generated
T6755 23505-23510 DT denotes these
T6756 23511-23519 NNS denotes plasmids
T6758 23523-23532 NNS denotes templates
T6759 23532-23534 , denotes ,
T6760 23534-23539 NN denotes sense
T6761 23554-23562 VBN denotes labelled
T6762 23540-23543 CC denotes and
T6763 23544-23553 JJ denotes antisense
T6764 23567-23573 NNS denotes probes
T6765 23563-23566 NN denotes RNA
T6766 23574-23578 VBD denotes were
T6767 23589-23591 IN denotes by
T6768 23592-23594 NN denotes T7
T6769 23599-23609 NN denotes polymerase
T6770 23595-23598 NN denotes RNA
T6771 23610-23613 CC denotes and
T6772 23614-23615 -LRB- denotes [
T6773 23620-23623 NN denotes UTP
T6774 23615-23619 NN denotes α35S
T6775 23619-23620 -RRB- denotes ]
T6776 23623-23624 . denotes .
T6777 23624-23718 sentence denotes Frozen brain and spinal cord from 2 month-old C57BL/6 female mice were used in this analysis.
T6778 23625-23631 VBN denotes Frozen
T6779 23632-23637 NN denotes brain
T6780 23696-23700 VBN denotes used
T6781 23638-23641 CC denotes and
T6782 23642-23648 JJ denotes spinal
T6783 23649-23653 NN denotes cord
T6784 23654-23658 IN denotes from
T6785 23659-23660 CD denotes 2
T6786 23661-23666 NN denotes month
T6787 23667-23670 JJ denotes old
T6788 23666-23667 HYPH denotes -
T6789 23686-23690 NNS denotes mice
T6790 23671-23676 NN denotes C57BL
T6791 23676-23677 HYPH denotes /
T6792 23677-23678 CD denotes 6
T6793 23679-23685 JJ denotes female
T6794 23691-23695 VBD denotes were
T6795 23701-23703 IN denotes in
T6796 23704-23708 DT denotes this
T6797 23709-23717 NN denotes analysis
T6798 23717-23718 . denotes .
T6799 23718-23848 sentence denotes Pretreatment, hybridisation, RNase treatment and washing was carried out following the protocol described in the literature [33].
T6800 23719-23731 NN denotes Pretreatment
T6801 23780-23787 VBN denotes carried
T6802 23731-23733 , denotes ,
T6803 23733-23746 NN denotes hybridisation
T6804 23746-23748 , denotes ,
T6805 23748-23753 NN denotes RNase
T6806 23754-23763 NN denotes treatment
T6807 23764-23767 CC denotes and
T6808 23768-23775 NN denotes washing
T6809 23776-23779 VBD denotes was
T6810 23788-23791 RP denotes out
T6811 23792-23801 VBG denotes following
T6812 23802-23805 DT denotes the
T6813 23806-23814 NN denotes protocol
T6814 23815-23824 VBN denotes described
T6815 23825-23827 IN denotes in
T6816 23828-23831 DT denotes the
T6817 23832-23842 NN denotes literature
T6818 23843-23844 -LRB- denotes [
T6819 23844-23846 CD denotes 33
T6820 23846-23847 -RRB- denotes ]
T6821 23847-23848 . denotes .
T6822 23848-24003 sentence denotes Dehydrated slides were attached to imaging plates for 48 hours and the autoradiograms were analysed using a Bio-Image Analyser (BAS3000, Fuji Photo Film).
T6823 23849-23859 VBN denotes Dehydrated
T6824 23860-23866 NNS denotes slides
T6825 23872-23880 VBN denotes attached
T6826 23867-23871 VBD denotes were
T6827 23881-23883 IN denotes to
T6828 23884-23891 NN denotes imaging
T6829 23892-23898 NNS denotes plates
T6830 23899-23902 IN denotes for
T6831 23903-23905 CD denotes 48
T6832 23906-23911 NNS denotes hours
T6833 23912-23915 CC denotes and
T6834 23916-23919 DT denotes the
T6835 23920-23934 NNS denotes autoradiograms
T6836 23940-23948 VBN denotes analysed
T6837 23935-23939 VBD denotes were
T6838 23949-23954 VBG denotes using
T6839 23955-23956 DT denotes a
T6840 23967-23975 NNP denotes Analyser
T6841 23957-23966 NNP denotes Bio-Image
T6842 23976-23977 -LRB- denotes (
T6843 23997-24001 NNP denotes Film
T6844 23977-23984 NN denotes BAS3000
T6845 23984-23986 , denotes ,
T6846 23986-23990 NNP denotes Fuji
T6847 23991-23996 NNP denotes Photo
T6848 24001-24002 -RRB- denotes )
T6849 24002-24003 . denotes .
T89 28-43 NN denotes hypomyelination
T596 1192-1193 DT denotes A
R4 T91 T85 prep in,Ataxia
R5 T92 T93 npadvmod ADAM22,deficient
R6 T93 T95 amod deficient,mice
R8 T95 T91 pobj mice,in
R9 T100 T101 nsubj ADAM22,is
R10 T102 T103 det a,member
R11 T103 T101 attr member,is
R12 T104 T103 prep of,member
R13 T105 T106 det the,family
R14 T106 T104 pobj family,of
R17 T109 T101 punct ", ",is
R18 T110 T101 cc but,is
R19 T111 T112 det the,fact
R20 T112 T113 nsubj fact,makes
R21 T113 T101 conj makes,is
R23 T115 T112 acl expressed,fact
R24 T116 T115 nsubjpass it,expressed
R25 T117 T115 auxpass is,expressed
R29 T121 T119 pobj systems,in
R30 T122 T121 amod nervous,systems
R31 T123 T124 nsubj it,unique
R32 T124 T113 ccomp unique,makes
R33 T125 T113 punct .,makes
R34 T127 T128 poss ADAM22,similarity
R35 T128 T131 nsubj similarity,suggests
R38 T132 T128 prep to,similarity
R39 T133 T134 amod other,ADAMs
R40 T134 T132 pobj ADAMs,to
R41 T135 T136 nsubj it,be
R42 T136 T131 ccomp be,suggests
R44 T138 T139 det an,binder
R45 T139 T136 attr binder,be
R47 T141 T136 cc and,be
R48 T142 T143 advmod thus,have
R49 T143 T136 conj have,be
R51 T145 T146 det a,role
R52 T146 T143 dobj role,have
R53 T147 T146 prep in,role
R54 T148 T149 nmod cell,cell
R55 T149 T151 nmod cell,interactions
R57 T151 T147 pobj interactions,in
R60 T154 T149 conj matrix,cell
R61 T155 T154 punct -,matrix
R62 T156 T131 punct .,suggests
R63 T158 T159 aux To,elucidate
R64 T159 T160 advcl elucidate,employed
R65 T161 T162 det the,functions
R66 T162 T159 dobj functions,elucidate
R68 T164 T162 prep of,functions
R69 T165 T164 pobj ADAM22,of
R70 T166 T160 punct ", ",employed
R71 T167 T160 nsubj we,employed
R72 T168 T169 compound gene,targeting
R73 T169 T160 dobj targeting,employed
R74 T170 T171 aux to,generate
R75 T171 T160 advcl generate,employed
R76 T172 T173 compound ADAM22,knockout
R77 T173 T174 compound knockout,mice
R78 T174 T171 dobj mice,generate
R79 T175 T160 punct .,employed
R80 T179 T180 npadvmod ADAM22,deficient
R81 T180 T182 amod deficient,mice
R83 T182 T183 nsubjpass mice,produced
R84 T184 T183 auxpass were,produced
R85 T185 T183 prep in,produced
R86 T186 T187 det a,accordance
R87 T187 T185 pobj accordance,in
R89 T189 T187 prep with,accordance
R90 T190 T191 det the,ratio
R91 T191 T189 pobj ratio,with
R93 T193 T183 cc and,produced
R94 T194 T183 conj appeared,produced
R95 T195 T194 oprd normal,appeared
R96 T196 T194 prep at,appeared
R97 T197 T196 pobj birth,at
R98 T198 T183 punct .,produced
R99 T200 T201 prep After,observed
R100 T202 T203 nummod one,week
R101 T203 T200 pobj week,After
R102 T204 T201 punct ", ",observed
R103 T205 T206 amod severe,ataxia
R104 T206 T201 nsubjpass ataxia,observed
R105 T207 T201 auxpass was,observed
R106 T208 T201 punct ", ",observed
R107 T209 T201 cc and,observed
R108 T210 T211 det all,homozygotes
R109 T211 T212 nsubj homozygotes,died
R110 T212 T201 conj died,observed
R111 T213 T212 prep before,died
R112 T214 T213 pobj weaning,before
R113 T215 T212 punct ", ",died
R114 T216 T217 advmod probably,due
R115 T217 T212 prep due,died
R116 T218 T217 pcomp to,due
R117 T219 T217 pobj convulsions,due
R118 T220 T201 punct .,observed
R119 T222 T223 det No,abnormalities
R120 T223 T226 nsubjpass abnormalities,detected
R123 T226 T228 ccomp detected,observed
R125 T229 T226 prep in,detected
R126 T230 T231 det the,cortex
R127 T231 T229 pobj cortex,in
R129 T233 T231 cc or,cortex
R130 T234 T231 conj cerebellum,cortex
R131 T235 T231 prep of,cortex
R132 T236 T237 det the,mutants
R133 T237 T235 pobj mutants,of
R135 T239 T228 punct ;,observed
R136 T240 T228 advmod however,observed
R137 T241 T228 punct ", ",observed
R138 T242 T243 amod marked,hypomyelination
R139 T243 T228 nsubjpass hypomyelination,observed
R140 T244 T243 prep of,hypomyelination
R141 T245 T246 det the,nerves
R142 T246 T244 pobj nerves,of
R144 T248 T228 auxpass was,observed
R145 T249 T228 punct .,observed
R146 T253 T254 det The,results
R147 T254 T255 nsubj results,demonstrate
R148 T256 T254 prep of,results
R149 T257 T258 poss our,study
R150 T258 T256 pobj study,of
R151 T259 T260 mark that,involved
R152 T260 T255 ccomp involved,demonstrate
R156 T264 T260 prep in,involved
R157 T265 T266 det the,functioning
R158 T266 T264 pobj functioning,in
R160 T268 T266 prep of,functioning
R161 T269 T270 det the,system
R162 T270 T268 pobj system,of
R164 T272 T255 punct .,demonstrate
R165 T274 T275 amod Further,analysis
R166 T275 T276 nsubj analysis,provide
R167 T277 T275 prep of,analysis
R168 T278 T277 pobj ADAM22,of
R169 T279 T276 aux will,provide
R170 T280 T276 dobj clues,provide
R171 T281 T280 prep to,clues
R172 T282 T281 pcomp understanding,to
R173 T283 T284 det the,mechanisms
R174 T284 T282 dobj mechanisms,understanding
R175 T285 T284 prep of,mechanisms
R176 T286 T287 amod human,diseases
R177 T287 T285 pobj diseases,of
R178 T288 T289 amod such,as
R179 T289 T287 prep as,diseases
R180 T290 T291 amod epileptic,seizures
R181 T291 T289 pobj seizures,as
R182 T292 T291 cc and,seizures
R183 T293 T294 amod peripheral,neuropathy
R184 T294 T291 conj neuropathy,seizures
R185 T295 T276 punct .,provide
R186 T89 T85 conj hypomyelination,Ataxia
R198 T605 T607 amod spanning,proteins
R199 T606 T605 punct -,spanning
R200 T607 T603 pobj proteins,of
R201 T608 T607 amod multi-domain,proteins
R204 T611 T609 dobj domain,containing
R205 T612 T613 npadvmod metalloproteinase,like
R207 T614 T613 punct -,like
R210 T617 T611 conj domain,domain
R211 T618 T619 npadvmod disintegrin,like
R213 T620 T619 punct -,like
R218 T627 T629 nummod 30,ADAMs
R219 T628 T627 quantmod than,30
R227 T637 T639 nsubj activities,implicate
R228 T638 T637 amod biological,activities
R251 T664 T662 dobj domains,shed
R252 T665 T664 amod extracellular,domains
R255 T668 T670 amod bound,factors
R256 T669 T668 punct -,bound
R257 T670 T666 pobj factors,of
R258 T671 T670 compound growth,factors
R262 T675 T662 parataxis 2,shed
R263 T676 T675 nummod 1,2
R264 T677 T675 punct ",",2
R283 T698 T696 pobj alpha,including
R284 T699 T698 compound necrosis,alpha
R285 T700 T698 compound factor,alpha
R287 T702 T698 parataxis 4,alpha
R288 T703 T702 nummod 3,4
R289 T704 T702 punct ",",4
R293 T708 T710 amod binding,factor
R294 T709 T708 punct -,binding
R295 T710 T698 conj factor,alpha
R296 T711 T712 amod epidermal,factor
R298 T713 T712 compound growth,factor
R300 T715 T714 punct -,like
R301 T716 T710 compound growth,factor
R303 T718 T710 parataxis 7,factor
R304 T719 T718 nummod 6,7
R305 T720 T718 punct ",",7
R309 T724 T710 conj alpha,factor
R310 T725 T724 compound growth,alpha
R311 T726 T724 compound factor,alpha
R319 T736 T738 amod null,mice
R320 T737 T736 punct -,null
R324 T741 T733 ccomp is,revealed
R325 T742 T741 nsubj ADAM17,is
R332 T749 T747 dobj role,plays
R333 T750 T749 amod essential,role
R341 T758 T733 parataxis 8,revealed
R342 T759 T758 nummod 6,8
R343 T760 T758 punct ",",8
R351 T770 T772 nmod cell,adhesion
R352 T771 T770 punct -,cell
R353 T772 T768 pobj adhesion,in
R354 T773 T770 cc or,cell
R355 T774 T775 compound cell,matrix
R357 T776 T775 punct -,matrix
R367 T787 T789 nummod 10,ADAMs
R368 T788 T787 quantmod than,10
R375 T796 T798 amod mediated,adhesion
R376 T797 T796 punct -,mediated
R377 T798 T794 dobj adhesion,support
R378 T799 T798 compound cell,adhesion
R389 T812 T808 ccomp are,reported
R390 T813 T814 npadvmod ADAM2,null
R392 T815 T814 punct -,null
R393 T816 T812 nsubj mutants,are
R394 T817 T814 cc and,null
R395 T818 T819 npadvmod ADAM3,null
R396 T819 T814 conj null,null
R397 T820 T819 punct -,null
R398 T821 T816 amod male,mutants
R405 T828 T830 amod binding,abilities
R406 T829 T828 punct -,binding
R421 T846 T840 advcl lack,are
R422 T847 T846 nsubj they,lack
R424 T849 T846 dobj sequences,lack
R425 T850 T849 compound site,sequences
R428 T853 T851 pobj domain,in
R429 T854 T853 compound metalloprotease,domain
R435 T862 T859 ccomp have,showed
R436 T863 T864 amod non-proteinase,members
R437 T864 T862 nsubj members,have
R438 T865 T864 prep of,members
R439 T866 T865 pobj ADAMs,of
R440 T867 T862 advmod also,have
R452 T881 T879 pobj genes,of
R453 T882 T880 punct ", ",ADAM11
R454 T883 T880 conj ADAM22,ADAM11
R455 T884 T883 cc and,ADAM22
R456 T885 T883 conj ADAM23,ADAM22
R459 T888 T878 conj expression,findings
R460 T889 T888 amod restricted,expression
R463 T892 T890 pobj systems,in
R464 T893 T892 amod human,systems
R465 T894 T893 cc and,human
R466 T895 T893 conj murine,human
R467 T896 T892 amod nervous,systems
R477 T908 T906 ccomp are,suggests
R478 T909 T908 nsubj they,are
R483 T914 T906 advcl lack,suggests
R484 T915 T914 nsubj they,lack
R485 T916 T915 appos all,they
R487 T918 T914 dobj motif,lack
R488 T919 T918 amod catalytic,motif
R494 T927 T923 ccomp localised,reported
R495 T928 T929 compound ADAM23,protein
R496 T929 T927 nsubjpass protein,localised
R497 T930 T927 auxpass is,localised
R500 T933 T931 pobj surface,to
R501 T934 T933 compound cell,surface
R509 T942 T944 nmod v,heterodimer
R510 T943 T942 punct -,v
R511 T944 T940 pobj heterodimer,with
R512 T945 T944 nmod beta,heterodimer
R513 T946 T945 punct -,beta
R514 T947 T945 nummod 3,beta
R515 T948 T944 compound integrin,heterodimer
R522 T955 T927 conj results,localised
R523 T956 T954 prep of,disruption
R524 T957 T958 compound ADAM23,gene
R525 T958 T956 pobj gene,of
R526 T959 T954 prep in,disruption
R527 T960 T961 det the,mouse
R528 T961 T959 pobj mouse,in
R542 T976 T984 advcl is,be
R543 T977 T978 det the,cause
R544 T978 T976 nsubj cause,is
R545 T979 T978 prep of,cause
R546 T980 T979 pobj death,of
R547 T981 T978 prep in,cause
R548 T982 T983 det this,mouse
R549 T983 T981 pobj mouse,in
R557 T992 T984 nsubj interactions,be
R558 T993 T994 nmod cell,cell
R560 T995 T994 punct -,cell
R561 T996 T994 cc or,cell
R562 T997 T998 compound cell,matrix
R564 T999 T998 punct -,matrix
R567 T1002 T1000 pobj system,in
R568 T1003 T1002 amod nervous,system
R585 T1022 T1020 pobj domains,in
R586 T1023 T1022 amod extracellular,domains
R594 T1033 T1031 pobj sequences,in
R595 T1034 T1033 amod putative,sequences
R596 T1035 T1036 npadvmod integrin,binding
R597 T1036 T1033 amod binding,sequences
R598 T1037 T1033 compound loop,sequences
R601 T1040 T1033 appos CD,sequences
R602 T1041 T1042 punct (,D
R603 T1042 T1039 parataxis D,CR
R604 T1043 T1042 compound E,D
R605 T1044 T1042 punct /,D
R606 T1045 T1042 punct ),D
R607 T1046 T1040 nmod AVN,CD
R608 T1047 T1048 punct (,D
R609 T1048 T1046 parataxis D,AVN
R610 T1049 T1048 compound E,D
R611 T1050 T1048 punct /,D
R612 T1051 T1048 punct ),D
R615 T1054 T1040 relcl located,CD
R616 T1055 T1054 auxpass is,located
R620 T1059 T1057 pobj domain,of
R621 T1060 T1059 compound disintegrin,domain
R629 T1070 T1068 ccomp is,hypothesize
R630 T1071 T1070 nsubj ADAM22,is
R632 T1073 T1070 attr binder,is
R633 T1074 T1073 compound integrin,binder
R637 T1078 T1076 dobj role,plays
R638 T1079 T1078 amod important,role
R641 T1082 T1080 pobj system,in
R642 T1083 T1082 amod nervous,system
R651 T1094 T1091 dobj functions,determine
R652 T1095 T1094 amod physiological,functions
R660 T1103 T1101 dobj mice,analysed
R661 T1104 T1105 npadvmod gene,targeted
R663 T1106 T1105 punct -,targeted
R667 T1244 T1243 prep of,Generation
R668 T1245 T1246 npadvmod ADAM22,deficient
R669 T1246 T1248 amod deficient,mice
R670 T1247 T1246 punct -,deficient
R671 T1248 T1244 pobj mice,of
R672 T1250 T1251 nsubjpass Mice,generated
R673 T1252 T1250 acl carrying,Mice
R674 T1253 T1254 det a,mutation
R675 T1254 T1252 dobj mutation,carrying
R676 T1255 T1254 amod targeted,mutation
R677 T1256 T1252 prep in,carrying
R678 T1257 T1258 poss their,gene
R679 T1258 T1256 pobj gene,in
R680 T1259 T1258 compound Adam22,gene
R681 T1260 T1251 auxpass were,generated
R682 T1261 T1251 prep by,generated
R683 T1262 T1263 amod homologous,recombination
R684 T1263 T1261 pobj recombination,by
R685 T1264 T1265 punct (,1A
R686 T1265 T1251 parataxis 1A,generated
R687 T1266 T1265 compound Fig.,1A
R688 T1267 T1265 punct ),1A
R689 T1268 T1251 punct .,generated
R690 T1270 T1271 amod Correct,events
R691 T1271 T1273 nsubjpass events,confirmed
R692 T1272 T1271 amod targeting,events
R693 T1274 T1273 auxpass were,confirmed
R694 T1275 T1273 prep by,confirmed
R695 T1276 T1277 compound Southern,blot
R696 T1277 T1278 compound blot,analysis
R697 T1278 T1275 pobj analysis,by
R698 T1279 T1280 punct (,1B
R699 T1280 T1273 parataxis 1B,confirmed
R700 T1281 T1280 compound Fig.,1B
R701 T1282 T1280 punct ),1B
R702 T1283 T1273 punct .,confirmed
R703 T1285 T1286 mark Since,introduced
R704 T1286 T1291 advcl introduced,synthesized
R705 T1287 T1288 det the,codon
R706 T1288 T1286 nsubjpass codon,introduced
R707 T1289 T1288 compound termination,codon
R708 T1290 T1286 auxpass was,introduced
R709 T1292 T1286 prep in,introduced
R710 T1293 T1292 pobj exon,in
R711 T1294 T1293 nummod 8,exon
R712 T1295 T1286 prep in,introduced
R713 T1296 T1297 det the,domain
R714 T1297 T1295 pobj domain,in
R715 T1298 T1297 amod pro-protein,domain
R716 T1299 T1291 punct ", ",synthesized
R717 T1300 T1301 advmod only,form
R719 T1302 T1301 det the,form
R720 T1303 T1301 amod truncated,form
R721 T1304 T1301 prep of,form
R722 T1305 T1306 det the,protein
R723 T1306 T1304 pobj protein,of
R724 T1307 T1306 compound ADAM22,protein
R725 T1308 T1291 aux would,synthesized
R726 T1309 T1291 auxpass be,synthesized
R727 T1310 T1291 prep from,synthesized
R728 T1311 T1312 det this,allele
R729 T1312 T1310 pobj allele,from
R730 T1313 T1312 amod targeted,allele
R731 T1314 T1291 punct .,synthesized
R732 T1316 T1317 mark Because,removed
R733 T1317 T1323 advcl removed,considered
R734 T1318 T1319 det a,domain
R735 T1319 T1317 nsubjpass domain,removed
R736 T1320 T1319 amod pro-protein,domain
R737 T1321 T1317 auxpass is,removed
R738 T1322 T1317 advmod always,removed
R739 T1324 T1317 prep in,removed
R740 T1325 T1326 det the,proteins
R741 T1326 T1324 pobj proteins,in
R742 T1327 T1326 amod mature,proteins
R743 T1328 T1326 amod functional,proteins
R744 T1329 T1326 compound ADAM,proteins
R745 T1330 T1326 punct -,proteins
R746 T1331 T1317 agent by,removed
R747 T1332 T1333 det the,proteases
R748 T1333 T1331 pobj proteases,by
R749 T1334 T1335 npadvmod Furin,like
R750 T1335 T1333 amod like,proteases
R751 T1336 T1335 punct -,like
R752 T1337 T1317 cc and,removed
R753 T1338 T1339 auxpass is,thought
R754 T1339 T1317 conj thought,removed
R755 T1340 T1341 aux to,be
R756 T1341 T1339 xcomp be,thought
R757 T1342 T1341 acomp non-functional,be
R758 T1343 T1341 npadvmod itself,be
R759 T1344 T1323 punct ", ",considered
R760 T1345 T1323 nsubj we,considered
R761 T1346 T1347 mark that,has
R762 T1347 T1323 ccomp has,considered
R763 T1348 T1349 det this,form
R764 T1349 T1347 nsubj form,has
R765 T1350 T1349 amod truncated,form
R766 T1351 T1349 prep of,form
R767 T1352 T1353 compound ADAM22,protein
R768 T1353 T1351 pobj protein,of
R769 T1354 T1355 det no,function
R770 T1355 T1347 dobj function,has
R771 T1356 T1323 punct .,considered
R772 T1358 T1359 nsubjpass Absence,confirmed
R773 T1360 T1358 prep of,Absence
R774 T1361 T1362 amod mature,protein
R775 T1362 T1360 pobj protein,of
R776 T1363 T1362 compound ADAM22,protein
R777 T1364 T1358 prep in,Absence
R778 T1365 T1366 amod homozygous,mutants
R779 T1366 T1364 pobj mutants,in
R780 T1367 T1359 auxpass was,confirmed
R781 T1368 T1359 prep by,confirmed
R782 T1369 T1370 compound Western,blot
R783 T1370 T1371 compound blot,analysis
R784 T1371 T1368 pobj analysis,by
R785 T1372 T1371 acl using,analysis
R786 T1373 T1374 det the,antibody
R787 T1374 T1372 dobj antibody,using
R788 T1375 T1374 amod specific,antibody
R789 T1376 T1374 punct ", ",antibody
R790 T1377 T1378 dep which,recognizes
R791 T1378 T1374 relcl recognizes,antibody
R792 T1379 T1380 det the,domain
R793 T1380 T1378 dobj domain,recognizes
R794 T1381 T1380 amod cytoplasmic,domain
R795 T1382 T1380 prep of,domain
R796 T1383 T1384 det the,protein
R797 T1384 T1382 pobj protein,of
R798 T1385 T1384 compound ADAM22,protein
R799 T1386 T1387 punct (,1C
R800 T1387 T1359 parataxis 1C,confirmed
R801 T1388 T1387 compound Fig.,1C
R802 T1389 T1387 punct ),1C
R803 T1390 T1359 punct .,confirmed
R804 T1392 T1393 amod Homozygous,mutants
R805 T1393 T1394 nsubj mutants,showed
R806 T1395 T1396 det no,defects
R807 T1396 T1394 dobj defects,showed
R808 T1397 T1396 amod noticeable,defects
R809 T1398 T1394 prep at,showed
R810 T1399 T1398 pobj birth,at
R811 T1400 T1394 cc and,showed
R812 T1401 T1394 conj were,showed
R813 T1402 T1401 acomp indistinguishable,were
R814 T1403 T1402 prep from,indistinguishable
R815 T1404 T1405 amod wild,type
R816 T1405 T1407 nmod type,littermates
R817 T1406 T1405 punct -,type
R819 T1408 T1405 cc or,type
R820 T1409 T1405 conj heterozygous,type
R821 T1410 T1401 prep during,were
R822 T1411 T1412 det the,week
R823 T1412 T1410 pobj week,during
R824 T1413 T1412 amod first,week
R825 T1414 T1394 punct .,showed
R826 T1416 T1417 prep At,were
R827 T1418 T1419 amod postnatal,day
R828 T1419 T1416 pobj day,At
R829 T1420 T1419 nummod 10,day
R830 T1421 T1419 punct (,day
R831 T1422 T1419 appos P10,day
R832 T1423 T1417 punct ),were
R833 T1424 T1417 punct ", ",were
R834 T1425 T1417 nsubj most,were
R835 T1426 T1425 prep of,most
R836 T1427 T1428 det the,mutants
R837 T1428 T1426 pobj mutants,of
R838 T1429 T1428 amod homozygous,mutants
R839 T1430 T1417 acomp distinguishable,were
R840 T1431 T1430 prep by,distinguishable
R841 T1432 T1431 pobj abnormalities,by
R842 T1433 T1434 amod such,as
R843 T1434 T1432 prep as,abnormalities
R844 T1435 T1436 amod reduced,weight
R845 T1436 T1434 pobj weight,as
R846 T1437 T1436 compound body,weight
R847 T1438 T1436 cc and,weight
R848 T1439 T1440 amod uncoordinated,movements
R849 T1440 T1436 conj movements,weight
R850 T1441 T1440 prep of,movements
R851 T1442 T1443 poss their,limbs
R852 T1443 T1441 pobj limbs,of
R853 T1444 T1417 punct .,were
R854 T1446 T1447 prep After,displayed
R855 T1448 T1446 pobj P10,After
R856 T1449 T1447 punct ", ",displayed
R857 T1450 T1451 det all,homozygotes
R858 T1451 T1447 nsubj homozygotes,displayed
R859 T1452 T1453 amod severe,ataxia
R860 T1453 T1447 dobj ataxia,displayed
R861 T1454 T1455 punct (,Fig.
R862 T1455 T1447 parataxis Fig.,displayed
R863 T1456 T1455 nummod 2,Fig.
R864 T1457 T1455 punct ),Fig.
R865 T1458 T1447 cc and,displayed
R866 T1459 T1447 conj began,displayed
R867 T1460 T1461 aux to,die
R868 T1461 T1459 xcomp die,began
R869 T1462 T1447 punct .,displayed
R870 T1464 T1465 aux To,measure
R871 T1465 T1466 advcl measure,backcrossed
R872 T1467 T1468 det the,rate
R873 T1468 T1465 dobj rate,measure
R874 T1469 T1468 compound survival,rate
R875 T1470 T1468 cc and,rate
R876 T1471 T1472 compound body,weight
R877 T1472 T1468 conj weight,rate
R878 T1473 T1468 prep of,rate
R879 T1474 T1475 det each,genotype
R880 T1475 T1473 pobj genotype,of
R881 T1476 T1465 advmod precisely,measure
R882 T1477 T1466 punct ", ",backcrossed
R883 T1478 T1466 nsubj we,backcrossed
R884 T1479 T1480 amod heterozygous,mutants
R885 T1480 T1466 dobj mutants,backcrossed
R886 T1481 T1480 amod male,mutants
R887 T1482 T1466 prep to,backcrossed
R888 T1483 T1484 nmod C57BL,females
R890 T1485 T1483 punct /,C57BL
R891 T1486 T1483 nummod 6,C57BL
R892 T1487 T1488 amod more,6
R893 T1488 T1490 nummod 6,times
R894 T1489 T1488 quantmod than,6
R895 T1490 T1466 npadvmod times,backcrossed
R896 T1491 T1466 punct .,backcrossed
R897 T1493 T1494 det The,males
R898 T1494 T1501 nsubjpass males,intercrossed
R899 T1495 T1494 amod resulting,males
R900 T1496 T1497 punct (,N
R901 T1497 T1494 parataxis N,males
R902 T1498 T1497 nummod 6,N
R903 T1499 T1497 punct ),N
R904 T1500 T1494 amod heterozygous,males
R905 T1502 T1494 cc and,males
R906 T1503 T1494 conj females,males
R907 T1504 T1501 auxpass were,intercrossed
R908 T1505 T1501 cc and,intercrossed
R909 T1506 T1507 det the,offspring
R910 T1507 T1509 nsubjpass offspring,analysed
R911 T1508 T1507 amod produced,offspring
R912 T1509 T1501 conj analysed,intercrossed
R913 T1510 T1509 auxpass were,analysed
R914 T1511 T1509 punct .,analysed
R915 T1513 T1514 det The,numbers
R916 T1514 T1515 nsubjpass numbers,shown
R917 T1516 T1514 prep of,numbers
R918 T1517 T1516 pobj survivors,of
R919 T1518 T1517 prep of,survivors
R920 T1519 T1520 det each,genotype
R921 T1520 T1518 pobj genotype,of
R922 T1521 T1522 det every,days
R923 T1522 T1514 npadvmod days,numbers
R924 T1523 T1522 nummod 5,days
R925 T1524 T1515 auxpass are,shown
R926 T1525 T1515 prep in,shown
R927 T1526 T1525 pobj Table,in
R928 T1527 T1526 nummod 1,Table
R929 T1528 T1515 punct .,shown
R930 T1530 T1531 prep At,was
R931 T1532 T1530 pobj P10,At
R932 T1533 T1531 punct ", ",was
R933 T1534 T1535 det the,ratio
R934 T1535 T1531 nsubj ratio,was
R935 T1536 T1535 prep of,ratio
R936 T1537 T1538 det each,genotype
R937 T1538 T1536 pobj genotype,of
R938 T1539 T1531 prep in,was
R939 T1540 T1541 amod close,accordance
R940 T1541 T1539 pobj accordance,in
R941 T1542 T1541 prep with,accordance
R942 T1543 T1544 det the,ratio
R943 T1544 T1542 pobj ratio,with
R944 T1545 T1544 amod Mendelian,ratio
R945 T1546 T1547 punct (,%
R946 T1547 T1541 parataxis %,accordance
R947 T1548 T1547 nummod 20.5,%
R948 T1549 T1547 punct +,%
R949 T1550 T1547 punct /,%
R950 T1551 T1547 punct +,%
R951 T1552 T1547 punct ", ",%
R952 T1553 T1554 nummod 55.1,%
R953 T1554 T1547 appos %,%
R954 T1555 T1554 punct +,%
R955 T1556 T1554 punct /,%
R956 T1557 T1554 punct -,%
R957 T1558 T1547 punct ", ",%
R958 T1559 T1560 nummod 24.4,%
R959 T1560 T1547 appos %,%
R960 T1561 T1560 punct -,%
R961 T1562 T1560 punct /,%
R962 T1563 T1560 punct -,%
R963 T1564 T1547 punct ;,%
R964 T1565 T1566 nsubj n,78
R965 T1566 T1547 ccomp 78,%
R966 T1567 T1566 punct =,78
R967 T1568 T1547 punct ),%
R968 T1569 T1531 punct .,was
R969 T1571 T1572 det This,result
R970 T1572 T1573 nsubj result,shows
R971 T1574 T1575 mark that,is
R972 T1575 T1573 ccomp is,shows
R973 T1576 T1575 nsubj ADAM22,is
R974 T1577 T1575 neg not,is
R975 T1578 T1575 acomp essential,is
R976 T1579 T1578 prep for,essential
R977 T1580 T1579 pobj embryogenesis,for
R978 T1581 T1573 punct .,shows
R979 T1583 T1584 prep At,was
R980 T1585 T1583 pobj P10,At
R981 T1586 T1584 punct ", ",was
R982 T1587 T1588 det the,weight
R984 T1589 T1588 amod average,weight
R985 T1590 T1588 compound body,weight
R986 T1591 T1588 prep of,weight
R987 T1592 T1593 amod homozygous,mutants
R988 T1593 T1591 pobj mutants,of
R989 T1594 T1595 advmod approximately,half
R990 T1595 T1584 attr half,was
R991 T1596 T1595 det that,half
R992 T1597 T1595 prep of,half
R993 T1598 T1599 amod wild,type
R994 T1599 T1601 nmod type,littermates
R995 T1600 T1599 punct -,type
R997 T1602 T1599 cc and,type
R998 T1603 T1599 conj heterozygous,type
R999 T1604 T1605 punct (,Table
R1000 T1605 T1584 parataxis Table,was
R1001 T1606 T1605 nummod 2,Table
R1002 T1607 T1605 punct ),Table
R1003 T1608 T1584 punct .,was
R1004 T1610 T1611 amod Homozygous,mutants
R1005 T1611 T1612 nsubj mutants,died
R1006 T1613 T1612 npadvmod one,died
R1007 T1614 T1613 prep by,one
R1008 T1615 T1614 pobj one,by
R1009 T1616 T1612 prep after,died
R1010 T1617 T1616 pobj P10,after
R1011 T1618 T1612 punct ", ",died
R1012 T1619 T1612 cc and,died
R1013 T1620 T1621 det all,homozygotes
R1014 T1621 T1622 nsubj homozygotes,died
R1015 T1622 T1612 conj died,died
R1016 T1623 T1622 prep before,died
R1017 T1624 T1623 pobj P20,before
R1018 T1625 T1622 punct .,died
R1019 T1627 T1628 prep Of,survived
R1020 T1629 T1630 amod more,100
R1021 T1630 T1632 nummod 100,homozygotes
R1022 T1631 T1630 quantmod than,100
R1023 T1632 T1627 pobj homozygotes,Of
R1024 T1633 T1634 nsubj we,produced
R1025 T1634 T1632 advcl produced,homozygotes
R1026 T1635 T1634 aux have,produced
R1027 T1636 T1628 punct ", ",survived
R1028 T1637 T1628 nsubj none,survived
R1029 T1638 T1628 aux have,survived
R1030 T1639 T1640 amod more,25
R1031 T1640 T1642 nummod 25,days
R1032 T1641 T1640 quantmod than,25
R1033 T1642 T1628 npadvmod days,survived
R1034 T1643 T1642 prep after,days
R1035 T1644 T1643 pobj birth,after
R1036 T1645 T1628 punct .,survived
R1037 T1647 T1648 advmod Meanwhile,looked
R1038 T1649 T1648 punct ", ",looked
R1039 T1650 T1651 amod heterozygous,mutants
R1040 T1651 T1648 nsubj mutants,looked
R1041 T1652 T1648 acomp normal,looked
R1042 T1653 T1648 punct ", ",looked
R1043 T1654 T1648 conj were,looked
R1044 T1655 T1654 acomp fertile,were
R1045 T1656 T1654 punct ", ",were
R1046 T1657 T1654 cc and,were
R1047 T1658 T1654 conj survived,were
R1048 T1659 T1658 prep for,survived
R1049 T1660 T1661 amod more,one
R1050 T1661 T1663 nummod one,year
R1051 T1662 T1661 quantmod than,one
R1052 T1663 T1659 pobj year,for
R1053 T1664 T1658 prep without,survived
R1054 T1665 T1666 amod obvious,defects
R1055 T1666 T1664 pobj defects,without
R1056 T1667 T1648 punct .,looked
R1059 T1961 T1960 prep of,Histopathology
R1060 T1962 T1963 npadvmod ADAM22,deficient
R1061 T1963 T1965 amod deficient,mice
R1062 T1964 T1963 punct -,deficient
R1063 T1965 T1961 pobj mice,of
R1064 T1967 T1968 nsubj We,reported
R1065 T1969 T1968 aux have,reported
R1066 T1970 T1971 det the,expression
R1067 T1971 T1968 dobj expression,reported
R1068 T1972 T1971 amod predominant,expression
R1069 T1973 T1971 prep of,expression
R1070 T1974 T1975 compound ADAM22,mRNA
R1071 T1975 T1973 pobj mRNA,of
R1072 T1976 T1971 prep in,expression
R1073 T1977 T1978 det the,brain
R1074 T1978 T1976 pobj brain,in
R1075 T1979 T1980 amod human,mouse
R1076 T1980 T1978 compound mouse,brain
R1077 T1981 T1980 cc and,mouse
R1078 T1982 T1968 prep by,reported
R1079 T1983 T1984 compound Northern,blot
R1080 T1984 T1985 compound blot,analysis
R1081 T1985 T1982 pobj analysis,by
R1082 T1986 T1987 punct [,14
R1083 T1987 T1968 parataxis 14,reported
R1084 T1988 T1987 nummod 13,14
R1085 T1989 T1987 punct ",",14
R1086 T1990 T1987 punct ],14
R1087 T1991 T1968 punct .,reported
R1088 T1993 T1994 aux To,determine
R1089 T1994 T1995 advcl determine,performed
R1090 T1996 T1997 det the,distribution
R1091 T1997 T1994 dobj distribution,determine
R1092 T1998 T1997 prep of,distribution
R1093 T1999 T2000 compound ADAM22,transcript
R1094 T2000 T1998 pobj transcript,of
R1095 T2001 T1994 prep in,determine
R1096 T2002 T2003 det the,CNS
R1097 T2003 T2001 pobj CNS,in
R1098 T2004 T2003 amod adult,CNS
R1099 T2005 T2003 compound mouse,CNS
R1100 T2006 T1994 advmod precisely,determine
R1101 T2007 T1995 punct ", ",performed
R1102 T2008 T2009 advmod in,situ
R1103 T2009 T2010 amod situ,analysis
R1104 T2010 T1995 nsubjpass analysis,performed
R1105 T2011 T2010 compound hybridisation,analysis
R1106 T2012 T1995 auxpass was,performed
R1107 T2013 T1995 advcl using,performed
R1108 T2014 T2015 advmod 35S,labeled
R1109 T2015 T2017 amod labeled,probe
R1110 T2016 T2015 punct -,labeled
R1111 T2017 T2013 dobj probe,using
R1112 T2018 T2017 compound RNA,probe
R1113 T2019 T1995 punct .,performed
R1114 T2021 T2022 mark As,shown
R1115 T2022 T2023 advcl shown,expressed
R1116 T2024 T2022 prep in,shown
R1117 T2025 T2024 pobj Fig.,in
R1118 T2026 T2025 nummod 3,Fig.
R1119 T2027 T2023 punct ", ",expressed
R1120 T2028 T2029 compound ADAM22,mRNA
R1121 T2029 T2023 nsubjpass mRNA,expressed
R1122 T2030 T2023 auxpass was,expressed
R1123 T2031 T2023 prep throughout,expressed
R1124 T2032 T2033 det the,CNS
R1125 T2033 T2031 pobj CNS,throughout
R1126 T2034 T2033 amod adult,CNS
R1127 T2035 T2033 compound mouse,CNS
R1128 T2036 T2023 punct .,expressed
R1129 T2038 T2039 amod Strong,signal
R1130 T2039 T2040 nsubjpass signal,detected
R1131 T2041 T2040 auxpass was,detected
R1132 T2042 T2040 prep in,detected
R1133 T2043 T2044 det the,cells
R1134 T2044 T2042 pobj cells,in
R1135 T2045 T2044 amod cerebellar,cells
R1136 T2046 T2044 compound granule,cells
R1137 T2047 T2044 cc and,cells
R1138 T2048 T2049 amod hippocampal,formation
R1139 T2049 T2044 conj formation,cells
R1140 T2050 T2040 punct .,detected
R1141 T2052 T2053 prep In,restricted
R1142 T2054 T2055 det the,cord
R1143 T2055 T2052 pobj cord,In
R1144 T2056 T2055 amod spinal,cord
R1145 T2057 T2053 punct ", ",restricted
R1146 T2058 T2059 compound hybridisation,signal
R1147 T2059 T2053 nsubjpass signal,restricted
R1148 T2060 T2053 auxpass was,restricted
R1149 T2061 T2053 prep to,restricted
R1150 T2062 T2063 det the,matter
R1151 T2063 T2061 pobj matter,to
R1152 T2064 T2063 amod grey,matter
R1153 T2065 T2053 punct .,restricted
R1154 T2067 T2068 det The,distribution
R1155 T2068 T2069 nsubj distribution,was
R1156 T2070 T2068 prep of,distribution
R1157 T2071 T2072 compound ADAM22,transcripts
R1158 T2072 T2070 pobj transcripts,of
R1159 T2073 T2068 prep in,distribution
R1160 T2074 T2075 det the,CNS
R1161 T2075 T2073 pobj CNS,in
R1162 T2076 T2077 advmod quite,similar
R1163 T2077 T2069 acomp similar,was
R1164 T2078 T2077 prep with,similar
R1165 T2079 T2078 pobj that,with
R1166 T2080 T2079 prep of,that
R1167 T2081 T2080 pobj ADAM11,of
R1168 T2082 T2081 punct ", ",ADAM11
R1169 T2083 T2084 poss whose,expression
R1170 T2084 T2086 dep expression,reported
R1171 T2085 T2084 amod neuronal,expression
R1172 T2086 T2081 relcl reported,ADAM11
R1173 T2087 T2086 aux has,reported
R1174 T2088 T2086 auxpass been,reported
R1175 T2089 T2090 punct [,19
R1176 T2090 T2069 parataxis 19,was
R1177 T2091 T2090 punct ],19
R1178 T2092 T2069 punct .,was
R1179 T2094 T2095 det These,results
R1180 T2095 T2096 nsubj results,suggest
R1181 T2097 T2098 mark that,is
R1183 T2099 T2100 det the,expression
R1184 T2100 T2098 nsubj expression,is
R1185 T2101 T2100 compound ADAM22,expression
R1186 T2102 T2100 compound mRNA,expression
R1187 T2103 T2098 acomp neuronal,is
R1188 T2104 T2098 prep in,is
R1189 T2105 T2106 det the,CNS
R1190 T2106 T2104 pobj CNS,in
R1191 T2107 T2106 compound mouse,CNS
R1192 T2108 T2096 punct .,suggest
R1193 T2110 T2111 prep Based,performed
R1194 T2112 T2110 prep on,Based
R1195 T2113 T2114 det the,pattern
R1196 T2114 T2112 pobj pattern,on
R1197 T2115 T2114 compound mRNA,pattern
R1198 T2116 T2114 compound distribution,pattern
R1199 T2117 T2111 punct ", ",performed
R1200 T2118 T2111 nsubj we,performed
R1201 T2119 T2120 amod immunohistopathological,analysis
R1202 T2120 T2111 dobj analysis,performed
R1203 T2121 T2120 prep of,analysis
R1204 T2122 T2123 det the,cerebellum
R1205 T2123 T2121 pobj cerebellum,of
R1206 T2124 T2123 cc and,cerebellum
R1207 T2125 T2123 conj hippocampus,cerebellum
R1208 T2126 T2111 advmod extensively,performed
R1209 T2127 T2111 punct .,performed
R1210 T2129 T2130 prep Despite,formed
R1211 T2131 T2132 det the,level
R1212 T2132 T2129 pobj level,Despite
R1213 T2133 T2132 amod high,level
R1214 T2134 T2132 prep of,level
R1215 T2135 T2134 pobj expression,of
R1216 T2136 T2135 prep of,expression
R1217 T2137 T2138 compound ADAM22,mRNAs
R1218 T2138 T2136 pobj mRNAs,of
R1219 T2139 T2132 prep in,level
R1220 T2140 T2141 det the,cells
R1221 T2141 T2139 pobj cells,in
R1222 T2142 T2141 amod cerebellar,cells
R1223 T2143 T2141 compound granule,cells
R1224 T2144 T2130 punct ", ",formed
R1225 T2145 T2146 compound granule,layer
R1226 T2146 T2130 nsubjpass layer,formed
R1227 T2147 T2146 compound cell,layer
R1228 T2148 T2130 auxpass was,formed
R1229 T2149 T2130 advmod normally,formed
R1230 T2150 T2130 cc and,formed
R1231 T2151 T2152 compound Purkinje,cell
R1232 T2152 T2153 compound cell,morphology
R1233 T2153 T2154 nsubj morphology,looked
R1234 T2154 T2130 conj looked,formed
R1235 T2155 T2156 punct (,staining
R1236 T2156 T2153 parataxis staining,morphology
R1237 T2157 T2156 npadvmod calbindin,staining
R1238 T2158 T2156 punct -,staining
R1239 T2159 T2156 punct ),staining
R1240 T2160 T2153 prep of,morphology
R1241 T2161 T2162 det the,mouse
R1242 T2162 T2160 pobj mouse,of
R1243 T2163 T2162 compound mutant,mouse
R1244 T2164 T2154 acomp intact,looked
R1245 T2165 T2166 punct (,4A
R1246 T2166 T2154 parataxis 4A,looked
R1247 T2167 T2166 compound Figs.,4A
R1248 T2168 T2169 punct –,D
R1249 T2169 T2166 prep D,4A
R1250 T2170 T2166 punct ),4A
R1251 T2171 T2130 punct .,formed
R1252 T2173 T2174 amod Hippocampal,formation
R1253 T2174 T2175 nsubjpass formation,formed
R1254 T2176 T2175 auxpass was,formed
R1255 T2177 T2175 advmod also,formed
R1256 T2178 T2175 advmod normally,formed
R1257 T2179 T2180 punct (,4I
R1258 T2180 T2175 parataxis 4I,formed
R1259 T2181 T2180 compound Figs.,4I
R1260 T2182 T2183 punct –,J
R1261 T2183 T2180 prep J,4I
R1262 T2184 T2180 punct ),4I
R1263 T2185 T2175 punct .,formed
R1264 T2187 T2188 det These,results
R1265 T2188 T2189 nsubj results,suggest
R1266 T2190 T2191 mark that,impaired
R1267 T2191 T2189 ccomp impaired,suggest
R1268 T2192 T2193 amod neuronal,cell
R1269 T2193 T2194 compound cell,migration
R1270 T2194 T2191 nsubjpass migration,impaired
R1271 T2195 T2191 auxpass was,impaired
R1272 T2196 T2191 neg not,impaired
R1273 T2197 T2191 prep in,impaired
R1274 T2198 T2199 det the,mouse
R1275 T2199 T2197 pobj mouse,in
R1276 T2200 T2199 compound mutant,mouse
R1277 T2201 T2189 punct .,suggest
R1278 T2203 T2204 compound Myelin,formation
R1279 T2204 T2206 nsubj formation,was
R1280 T2205 T2204 punct -,formation
R1281 T2207 T2204 acl detected,formation
R1282 T2208 T2207 prep by,detected
R1283 T2209 T2210 nmod MBP,staining
R1284 T2210 T2208 pobj staining,by
R1285 T2211 T2209 punct (,MBP
R1286 T2212 T2213 nmod myelin,protein
R1287 T2213 T2209 appos protein,MBP
R1288 T2214 T2213 amod basic,protein
R1289 T2215 T2210 punct ),staining
R1290 T2216 T2210 prep of,staining
R1291 T2217 T2218 det the,mutant
R1292 T2218 T2216 pobj mutant,of
R1293 T2219 T2204 prep in,formation
R1294 T2220 T2221 det the,cerebellum
R1295 T2221 T2219 pobj cerebellum,in
R1296 T2222 T2223 punct (,4H
R1297 T2223 T2221 parataxis 4H,cerebellum
R1298 T2224 T2223 compound Fig.,4H
R1299 T2225 T2223 punct ),4H
R1300 T2226 T2221 cc and,cerebellum
R1301 T2227 T2228 amod spinal,cord
R1302 T2228 T2221 conj cord,cerebellum
R1303 T2229 T2230 punct (,4L
R1304 T2230 T2228 parataxis 4L,cord
R1305 T2231 T2230 compound Fig.,4L
R1306 T2232 T2230 punct ),4L
R1307 T2233 T2206 advmod also,was
R1308 T2234 T2206 acomp indistinguishable,was
R1309 T2235 T2234 prep with,indistinguishable
R1310 T2236 T2237 det the,littermate
R1311 T2237 T2235 pobj littermate,with
R1312 T2238 T2239 amod wild,type
R1313 T2239 T2237 compound type,littermate
R1314 T2240 T2239 punct -,type
R1315 T2241 T2242 punct (,K
R1316 T2242 T2206 parataxis K,was
R1317 T2243 T2242 nmod Figs.,K
R1318 T2244 T2242 nmod 4G,K
R1319 T2245 T2242 punct ",",K
R1320 T2246 T2242 punct ),K
R1321 T2247 T2206 punct .,was
R1322 T2249 T2250 prep In,find
R1323 T2251 T2249 pobj summary,In
R1324 T2252 T2250 punct ", ",find
R1325 T2253 T2250 nsubj we,find
R1326 T2254 T2250 aux could,find
R1327 T2255 T2250 neg not,find
R1328 T2256 T2257 det any,signs
R1329 T2257 T2250 dobj signs,find
R1330 T2258 T2257 prep of,signs
R1331 T2259 T2258 pobj abnormalities,of
R1332 T2260 T2250 prep in,find
R1333 T2261 T2262 det the,brain
R1334 T2262 T2260 pobj brain,in
R1335 T2263 T2262 compound mutant,brain
R1336 T2264 T2262 compound mouse,brain
R1337 T2265 T2250 prep by,find
R1338 T2266 T2267 det the,examination
R1339 T2267 T2265 pobj examination,by
R1340 T2268 T2269 npadvmod light,microscopic
R1341 T2269 T2267 amod microscopic,examination
R1342 T2270 T2250 punct .,find
R1343 T2272 T2273 advmod Next,analysed
R1344 T2274 T2273 punct ", ",analysed
R1345 T2275 T2276 det the,cord
R1346 T2276 T2273 nsubjpass cord,analysed
R1347 T2277 T2276 amod spinal,cord
R1348 T2278 T2276 cc and,cord
R1349 T2279 T2280 amod peripheral,nerves
R1350 T2280 T2276 conj nerves,cord
R1351 T2281 T2276 prep of,cord
R1352 T2282 T2283 det each,genotype
R1353 T2283 T2281 pobj genotype,of
R1354 T2284 T2273 auxpass were,analysed
R1355 T2285 T2273 prep by,analysed
R1356 T2286 T2287 nmod toluidine,stain
R1357 T2287 T2285 pobj stain,by
R1358 T2288 T2287 amod blue,stain
R1359 T2289 T2287 punct ", ",stain
R1360 T2290 T2291 dep which,reveals
R1361 T2291 T2287 relcl reveals,stain
R1362 T2292 T2293 compound myelin,formation
R1363 T2293 T2291 dobj formation,reveals
R1364 T2294 T2273 punct .,analysed
R1365 T2296 T2297 advmod Surprisingly,observed
R1366 T2298 T2297 punct ", ",observed
R1367 T2299 T2297 nsubjpass lack,observed
R1368 T2300 T2299 prep of,lack
R1369 T2301 T2300 pobj myelin,of
R1370 T2302 T2301 cc or,myelin
R1371 T2303 T2304 amod thin,myelin
R1372 T2304 T2301 conj myelin,myelin
R1373 T2305 T2297 auxpass was,observed
R1374 T2306 T2297 prep in,observed
R1375 T2307 T2308 det the,nerves
R1376 T2308 T2306 pobj nerves,in
R1377 T2309 T2308 amod sciatic,nerves
R1378 T2310 T2311 punct (,5B
R1379 T2311 T2308 parataxis 5B,nerves
R1380 T2312 T2311 compound Fig.,5B
R1381 T2313 T2311 punct ),5B
R1382 T2314 T2308 cc and,nerves
R1383 T2315 T2316 amod trigeminal,nerves
R1384 T2316 T2308 conj nerves,nerves
R1385 T2317 T2318 punct (,5D
R1386 T2318 T2316 parataxis 5D,nerves
R1387 T2319 T2318 compound Fig.,5D
R1388 T2320 T2318 punct ),5D
R1389 T2321 T2297 prep in,observed
R1390 T2322 T2323 amod homozygous,mutants
R1391 T2323 T2321 pobj mutants,in
R1392 T2324 T2297 punct .,observed
R1393 T2326 T2327 mark Because,observed
R1394 T2327 T2331 advcl observed,suggested
R1395 T2328 T2329 det no,lesions
R1396 T2329 T2327 nsubjpass lesions,observed
R1397 T2330 T2327 auxpass were,observed
R1398 T2332 T2327 prep in,observed
R1399 T2333 T2334 det the,cord
R1400 T2334 T2332 pobj cord,in
R1401 T2335 T2334 amod spinal,cord
R1402 T2336 T2337 punct (,5F
R1403 T2337 T2327 parataxis 5F,observed
R1404 T2338 T2337 compound Fig.,5F
R1405 T2339 T2337 punct ),5F
R1406 T2340 T2331 punct ", ",suggested
R1407 T2341 T2331 nsubjpass it,suggested
R1408 T2342 T2331 auxpass was,suggested
R1409 T2343 T2344 mark that,occurred
R1410 T2344 T2331 ccomp occurred,suggested
R1411 T2345 T2346 compound Schwann,cell
R1412 T2346 T2347 npadvmod cell,specific
R1413 T2347 T2348 amod specific,defect
R1414 T2348 T2344 nsubj defect,occurred
R1415 T2349 T2348 compound myelination,defect
R1416 T2350 T2344 prep in,occurred
R1417 T2351 T2352 det the,mice
R1418 T2352 T2350 pobj mice,in
R1419 T2353 T2352 amod homozygous,mice
R1420 T2354 T2331 punct .,suggested
R1421 T2356 T2357 aux To,analyse
R1422 T2357 T2358 advcl analyse,performed
R1423 T2359 T2360 det the,state
R1424 T2360 T2357 dobj state,analyse
R1425 T2361 T2360 prep of,state
R1426 T2362 T2363 amod myelinating,cells
R1427 T2363 T2361 pobj cells,of
R1428 T2364 T2363 compound Schwann,cells
R1429 T2365 T2363 cc and,cells
R1430 T2366 T2363 conj axons,cells
R1431 T2367 T2358 punct ", ",performed
R1432 T2368 T2369 npadvmod electron,microscopic
R1433 T2369 T2370 amod microscopic,analysis
R1434 T2370 T2358 nsubjpass analysis,performed
R1435 T2371 T2372 punct (,EM
R1436 T2372 T2369 parataxis EM,microscopic
R1437 T2373 T2372 punct ),EM
R1438 T2374 T2370 prep of,analysis
R1439 T2375 T2376 det the,nerve
R1440 T2376 T2374 pobj nerve,of
R1441 T2377 T2376 amod sciatic,nerve
R1442 T2378 T2358 auxpass was,performed
R1443 T2379 T2380 punct (,Fig.
R1444 T2380 T2358 parataxis Fig.,performed
R1445 T2381 T2380 nummod 6,Fig.
R1446 T2382 T2380 punct ),Fig.
R1447 T2383 T2358 punct .,performed
R1448 T2385 T2386 compound Schwann,cells
R1449 T2386 T2387 nsubj cells,formed
R1450 T2388 T2387 dobj thin,formed
R1451 T2389 T2388 cc or,thin
R1452 T2390 T2391 nmod no,myelin
R1453 T2391 T2388 conj myelin,thin
R1454 T2392 T2387 prep in,formed
R1455 T2393 T2394 det the,mutant
R1456 T2394 T2392 pobj mutant,in
R1457 T2395 T2394 amod homozygous,mutant
R1458 T2396 T2397 punct (,6B
R1459 T2397 T2387 parataxis 6B,formed
R1460 T2398 T2397 compound Fig.,6B
R1461 T2399 T2397 punct ),6B
R1462 T2400 T2387 punct .,formed
R1463 T2402 T2403 prep In,showed
R1464 T2404 T2402 pobj contrast,In
R1465 T2405 T2403 punct ", ",showed
R1466 T2406 T2407 amod heterozygous,mice
R1467 T2407 T2403 nsubj mice,showed
R1468 T2408 T2409 amod complete,myelination
R1469 T2409 T2403 dobj myelination,showed
R1470 T2410 T2411 punct (,6A
R1471 T2411 T2403 parataxis 6A,showed
R1472 T2412 T2411 compound Fig.,6A
R1473 T2413 T2411 punct ),6A
R1474 T2414 T2403 punct .,showed
R1475 T2416 T2417 nsubj Morphology,looked
R1476 T2418 T2416 prep of,Morphology
R1477 T2419 T2420 det the,axons
R1478 T2420 T2418 pobj axons,of
R1479 T2421 T2417 acomp normal,looked
R1480 T2422 T2417 prep in,looked
R1481 T2423 T2424 det each,genotype
R1482 T2424 T2422 pobj genotype,in
R1483 T2425 T2417 punct .,looked
R1484 T2427 T2428 amod Immunohistochemical,analysis
R1485 T2428 T2429 nsubj analysis,showed
R1486 T2430 T2428 prep of,analysis
R1487 T2431 T2432 det the,nerve
R1488 T2432 T2430 pobj nerve,of
R1489 T2433 T2432 amod sciatic,nerve
R1490 T2434 T2435 mark that,were
R1491 T2435 T2429 ccomp were,showed
R1492 T2436 T2437 amod increased,number
R1493 T2437 T2435 nsubj number,were
R1494 T2438 T2437 prep of,number
R1495 T2439 T2438 pobj nuclei,of
R1496 T2440 T2441 punct (,7B
R1497 T2441 T2437 parataxis 7B,number
R1498 T2442 T2441 compound Fig.,7B
R1499 T2443 T2441 punct ),7B
R1500 T2444 T2437 cc and,number
R1501 T2445 T2446 amod reduced,staining
R1502 T2446 T2437 conj staining,number
R1503 T2447 T2446 prep of,staining
R1504 T2448 T2447 pobj MBP,of
R1505 T2449 T2450 punct (,7D
R1506 T2450 T2446 parataxis 7D,staining
R1507 T2451 T2450 compound Fig.,7D
R1508 T2452 T2450 punct ),7D
R1509 T2453 T2435 acomp remarkable,were
R1510 T2454 T2435 prep in,were
R1511 T2455 T2456 det the,mutant
R1512 T2456 T2454 pobj mutant,in
R1513 T2457 T2456 amod homozygous,mutant
R1514 T2458 T2429 punct .,showed
R1515 T2460 T2461 det The,marker
R1516 T2461 T2464 nsubjpass marker,observed
R1517 T2462 T2463 compound Schwann,cell
R1518 T2463 T2461 compound cell,marker
R1519 T2465 T2461 punct ", ",marker
R1520 T2466 T2467 compound S100,signal
R1521 T2467 T2461 appos signal,marker
R1522 T2468 T2467 compound staining,signal
R1523 T2469 T2464 auxpass was,observed
R1524 T2470 T2464 advmod intensely,observed
R1525 T2471 T2464 prep in,observed
R1526 T2472 T2473 det the,homozygote
R1527 T2473 T2471 pobj homozygote,in
R1528 T2474 T2475 advmod as,as
R1529 T2475 T2473 cc as,homozygote
R1530 T2476 T2475 advmod well,as
R1531 T2477 T2478 amod wild,type
R1532 T2478 T2473 conj type,homozygote
R1533 T2479 T2478 punct -,type
R1534 T2480 T2481 punct (,F
R1535 T2481 T2464 parataxis F,observed
R1536 T2482 T2481 nmod Figs.,F
R1537 T2483 T2481 nmod 7E,F
R1538 T2484 T2481 punct ", ",F
R1539 T2485 T2481 punct ),F
R1540 T2486 T2464 punct .,observed
R1541 T2488 T2489 det These,results
R1542 T2489 T2490 nsubj results,suggest
R1543 T2491 T2492 mark that,was
R1544 T2492 T2490 ccomp was,suggest
R1545 T2493 T2492 nsubj proliferation,was
R1546 T2494 T2493 prep of,proliferation
R1547 T2495 T2496 det the,cells
R1548 T2496 T2494 pobj cells,of
R1549 T2497 T2496 compound Schwann,cells
R1550 T2498 T2492 neg not,was
R1551 T2499 T2492 acomp impaired,was
R1552 T2500 T2492 cc but,was
R1553 T2501 T2502 nsubj differentiation,is
R1554 T2502 T2492 conj is,was
R1555 T2503 T2504 advmod severely,delayed
R1556 T2504 T2502 acomp delayed,is
R1557 T2505 T2502 prep in,is
R1558 T2506 T2507 det the,mice
R1559 T2507 T2505 pobj mice,in
R1560 T2508 T2509 npadvmod ADAM22,deficient
R1561 T2509 T2507 amod deficient,mice
R1562 T2510 T2509 punct -,deficient
R1563 T2511 T2490 punct .,suggest
R1568 T2746 T2747 amod Novel,variants
R1569 T2747 T2750 nsubj variants,isolated
R1570 T2748 T2747 compound ADAM22,variants
R1571 T2749 T2747 compound transcript,variants
R1572 T2751 T2750 prep from,isolated
R1573 T2752 T2753 det the,system
R1574 T2753 T2751 pobj system,from
R1575 T2754 T2753 amod peripheral,system
R1576 T2755 T2753 amod nervous,system
R1577 T2757 T2758 prep In,reported
R1578 T2759 T2760 det the,case
R1579 T2760 T2757 pobj case,In
R1580 T2761 T2760 prep of,case
R1581 T2762 T2763 amod human,ADAM22
R1582 T2763 T2761 pobj ADAM22,of
R1583 T2764 T2758 punct ", ",reported
R1584 T2765 T2758 nsubjpass isolation,reported
R1585 T2766 T2765 prep of,isolation
R1586 T2767 T2768 nummod five,variants
R1587 T2768 T2766 pobj variants,of
R1588 T2769 T2768 compound splicing,variants
R1589 T2770 T2765 cc and,isolation
R1590 T2771 T2765 conj existence,isolation
R1591 T2772 T2771 prep of,existence
R1592 T2773 T2774 nummod two,exons
R1593 T2774 T2772 pobj exons,of
R1594 T2775 T2774 amod terminating,exons
R1595 T2776 T2758 aux have,reported
R1596 T2777 T2758 auxpass been,reported
R1597 T2778 T2779 punct [,21
R1598 T2779 T2758 parataxis 21,reported
R1599 T2780 T2779 nummod 13,21
R1600 T2781 T2779 punct ",",21
R1601 T2782 T2779 nummod 20,21
R1602 T2783 T2779 punct ",",21
R1603 T2784 T2779 punct ],21
R1604 T2785 T2758 punct .,reported
R1605 T2787 T2788 advmod However,identified
R1606 T2789 T2788 punct ", ",identified
R1607 T2790 T2791 det the,exon
R1608 T2791 T2788 nsubjpass exon,identified
R1609 T2792 T2791 amod latter,exon
R1610 T2793 T2791 amod terminating,exon
R1611 T2794 T2791 punct (,exon
R1612 T2795 T2791 appos exon,exon
R1613 T2796 T2795 nummod 31,exon
R1614 T2797 T2791 punct ),exon
R1615 T2798 T2791 prep of,exon
R1616 T2799 T2800 det the,species
R1617 T2800 T2798 pobj species,of
R1618 T2801 T2800 compound mouse,species
R1619 T2802 T2788 aux has,identified
R1620 T2803 T2788 neg not,identified
R1621 T2804 T2788 auxpass been,identified
R1622 T2805 T2788 advmod yet,identified
R1623 T2806 T2788 punct .,identified
R1624 T2808 T2809 aux To,isolate
R1625 T2809 T2810 advcl isolate,sought
R1626 T2811 T2812 det the,form
R1627 T2812 T2809 dobj form,isolate
R1628 T2813 T2812 amod long,form
R1629 T2814 T2812 prep of,form
R1630 T2815 T2816 compound mouse,ADAM22
R1631 T2816 T2817 compound ADAM22,transcripts
R1632 T2817 T2814 pobj transcripts,of
R1633 T2818 T2810 punct ", ",sought
R1634 T2819 T2810 prep at,sought
R1635 T2820 T2819 pcomp first,at
R1636 T2821 T2810 punct ", ",sought
R1637 T2822 T2810 nsubj we,sought
R1638 T2823 T2824 det the,genome
R1639 T2824 T2810 dobj genome,sought
R1640 T2825 T2824 compound mouse,genome
R1641 T2826 T2810 prep by,sought
R1642 T2827 T2828 compound BLAST,homology
R1643 T2828 T2829 compound homology,search
R1644 T2829 T2826 pobj search,by
R1645 T2830 T2810 punct .,sought
R1646 T2832 T2833 compound Search,results
R1647 T2833 T2834 nsubj results,showed
R1648 T2835 T2836 mark that,contained
R1649 T2836 T2834 ccomp contained,showed
R1650 T2837 T2838 det the,genome
R1651 T2838 T2836 nsubj genome,contained
R1652 T2839 T2838 compound mouse,genome
R1653 T2840 T2841 compound contig,NT_039297
R1654 T2841 T2838 appos NT_039297,genome
R1655 T2842 T2836 dobj most,contained
R1656 T2843 T2842 prep of,most
R1657 T2844 T2845 det the,gene
R1658 T2845 T2843 pobj gene,of
R1659 T2846 T2845 compound mouse,gene
R1660 T2847 T2845 compound Adam22,gene
R1661 T2848 T2834 punct .,showed
R1662 T2850 T2851 prep In,found
R1663 T2851 T2857 ccomp found,were
R1664 T2852 T2853 det this,sequence
R1665 T2853 T2850 pobj sequence,In
R1666 T2854 T2853 compound contig,sequence
R1667 T2855 T2851 punct ", ",found
R1668 T2856 T2851 nsubj we,found
R1669 T2858 T2859 amod putative,30
R1670 T2859 T2851 dobj 30,found
R1671 T2860 T2859 nmod exons,30
R1672 T2861 T2859 cc and,30
R1673 T2862 T2859 conj 31,30
R1674 T2863 T2857 punct ", ",were
R1675 T2864 T2865 det those,sequences
R1676 T2865 T2857 nsubj sequences,were
R1677 T2866 T2867 advmod quite,similar
R1678 T2867 T2857 acomp similar,were
R1679 T2868 T2867 prep to,similar
R1680 T2869 T2870 amod human,isoform
R1681 T2870 T2868 pobj isoform,to
R1682 T2871 T2870 compound ADAM22,isoform
R1683 T2872 T2870 nummod 1,isoform
R1684 T2873 T2874 punct (,NM_021723
R1685 T2874 T2870 parataxis NM_021723,isoform
R1686 T2875 T2874 nmod GenBank,NM_021723
R1687 T2876 T2874 punct #,NM_021723
R1688 T2877 T2874 punct ),NM_021723
R1689 T2878 T2857 punct .,were
R1690 T2880 T2881 aux To,confirm
R1691 T2881 T2882 advcl confirm,designed
R1692 T2883 T2881 dobj existence,confirm
R1693 T2884 T2883 prep of,existence
R1694 T2885 T2884 pobj transcripts,of
R1695 T2886 T2882 punct ", ",designed
R1696 T2887 T2882 nsubj we,designed
R1697 T2888 T2882 dobj primers,designed
R1698 T2889 T2888 prep on,primers
R1699 T2890 T2891 det the,exon
R1700 T2891 T2889 pobj exon,on
R1701 T2892 T2891 amod putative,exon
R1702 T2893 T2891 nummod 31,exon
R1703 T2894 T2882 cc and,designed
R1704 T2895 T2882 conj performed,designed
R1705 T2896 T2897 compound RT,PCR
R1706 T2897 T2895 dobj PCR,performed
R1707 T2898 T2897 punct -,PCR
R1708 T2899 T2895 advcl using,performed
R1709 T2900 T2901 compound mouse,mRNA
R1710 T2901 T2899 dobj mRNA,using
R1711 T2902 T2901 compound cerebellum,mRNA
R1712 T2903 T2899 prep as,using
R1713 T2904 T2905 det a,template
R1714 T2905 T2903 pobj template,as
R1715 T2906 T2882 punct .,designed
R1716 T2908 T2909 compound TA,cloning
R1717 T2909 T2911 nsubj cloning,showed
R1718 T2910 T2909 punct -,cloning
R1719 T2912 T2909 prep of,cloning
R1720 T2913 T2914 det the,fragments
R1721 T2914 T2912 pobj fragments,of
R1722 T2915 T2914 amod amplified,fragments
R1723 T2916 T2909 cc and,cloning
R1724 T2917 T2918 compound sequencing,analysis
R1725 T2918 T2909 conj analysis,cloning
R1726 T2919 T2920 mark that,were
R1727 T2920 T2911 ccomp were,showed
R1728 T2921 T2922 quantmod 5,8
R1729 T2922 T2924 nummod 8,clones
R1730 T2923 T2922 quantmod of,8
R1731 T2924 T2920 nsubj clones,were
R1732 T2925 T2926 nmod type,isoform
R1733 T2926 T2920 attr isoform,were
R1734 T2927 T2925 nummod 1,type
R1735 T2928 T2929 punct (,G01
R1736 T2929 T2920 parataxis G01,were
R1737 T2930 T2929 punct ),G01
R1738 T2931 T2920 punct ", ",were
R1739 T2932 T2933 nummod 2,clones
R1740 T2933 T2934 nsubj clones,were
R1741 T2934 T2920 conj were,were
R1742 T2935 T2936 nmod type,isoform
R1743 T2936 T2934 attr isoform,were
R1744 T2937 T2935 nummod 3,type
R1745 T2938 T2939 punct (,G03
R1746 T2939 T2934 parataxis G03,were
R1747 T2940 T2939 punct ),G03
R1748 T2941 T2934 cc and,were
R1749 T2942 T2943 nummod 1,clone
R1750 T2943 T2944 nsubj clone,contained
R1751 T2944 T2934 conj contained,were
R1752 T2945 T2946 punct (,G08
R1753 T2946 T2943 parataxis G08,clone
R1754 T2947 T2946 punct ),G08
R1755 T2948 T2949 det a,exon
R1756 T2949 T2944 dobj exon,contained
R1757 T2950 T2949 amod novel,exon
R1758 T2951 T2949 prep between,exon
R1759 T2952 T2953 nmod exons,29
R1760 T2953 T2951 pobj 29,between
R1761 T2954 T2953 cc and,29
R1762 T2955 T2953 conj 30,29
R1763 T2956 T2911 punct .,showed
R1764 T2958 T2959 advmod Next,performed
R1765 T2960 T2959 punct ", ",performed
R1766 T2961 T2959 nsubj we,performed
R1767 T2962 T2963 compound RT,PCR
R1768 T2963 T2965 compound PCR,analysis
R1769 T2964 T2963 punct -,PCR
R1770 T2965 T2959 dobj analysis,performed
R1771 T2966 T2965 prep of,analysis
R1772 T2967 T2966 pobj mRNAs,of
R1773 T2968 T2967 acl purified,mRNAs
R1774 T2969 T2968 prep from,purified
R1775 T2970 T2971 amod several,tissues
R1776 T2971 T2969 pobj tissues,from
R1777 T2972 T2971 amod adult,tissues
R1778 T2973 T2971 compound mouse,tissues
R1779 T2974 T2971 punct ", ",tissues
R1780 T2975 T2976 amod such,as
R1781 T2976 T2971 prep as,tissues
R1782 T2977 T2978 det the,cord
R1783 T2978 T2976 pobj cord,as
R1784 T2979 T2978 amod spinal,cord
R1785 T2980 T2978 punct ", ",cord
R1786 T2981 T2982 amod dorsal,root
R1787 T2982 T2983 compound root,ganglion
R1788 T2983 T2978 conj ganglion,cord
R1789 T2984 T2983 punct (,ganglion
R1790 T2985 T2983 appos DRG,ganglion
R1791 T2986 T2983 punct ),ganglion
R1792 T2987 T2983 punct ", ",ganglion
R1793 T2988 T2989 amod sciatic,nerve
R1794 T2989 T2983 conj nerve,ganglion
R1795 T2990 T2989 cc and,nerve
R1796 T2991 T2992 amod cultured,cells
R1797 T2992 T2989 conj cells,nerve
R1798 T2993 T2992 amod primary,cells
R1799 T2994 T2992 compound Schwann,cells
R1800 T2995 T2959 punct .,performed
R1801 T2997 T2998 advmod Interestingly,amplified
R1802 T2999 T2998 punct ", ",amplified
R1803 T3000 T3001 amod multiple,fragments
R1804 T3001 T2998 nsubjpass fragments,amplified
R1805 T3002 T3001 compound DNA,fragments
R1806 T3003 T2998 auxpass were,amplified
R1807 T3004 T2998 prep from,amplified
R1808 T3005 T3006 det each,tissue
R1809 T3006 T3004 pobj tissue,from
R1810 T3007 T3008 punct (,Fig.
R1811 T3008 T2998 parataxis Fig.,amplified
R1812 T3009 T3008 nummod 8,Fig.
R1813 T3010 T3008 punct ),Fig.
R1814 T3011 T2998 punct ", ",amplified
R1815 T3012 T2998 cc and,amplified
R1816 T3013 T3014 nsubj length,was
R1817 T3014 T2998 conj was,amplified
R1818 T3015 T3013 prep of,length
R1819 T3016 T3017 amod major,fragments
R1820 T3017 T3015 pobj fragments,of
R1821 T3018 T3013 prep in,length
R1822 T3019 T3020 det each,lane
R1823 T3020 T3018 pobj lane,in
R1824 T3021 T3014 acomp different,was
R1825 T3022 T3014 punct .,was
R1826 T3024 T3025 prep For,were
R1827 T3026 T3024 pobj example,For
R1828 T3027 T3025 punct ", ",were
R1829 T3028 T3025 nsubj length,were
R1830 T3029 T3028 prep of,length
R1831 T3030 T3031 amod major,fragments
R1832 T3031 T3029 pobj fragments,of
R1833 T3032 T3031 amod amplified,fragments
R1834 T3033 T3031 compound PCR,fragments
R1835 T3034 T3031 prep from,fragments
R1836 T3035 T3036 det the,nerve
R1837 T3036 T3034 pobj nerve,from
R1838 T3037 T3036 amod sciatic,nerve
R1839 T3038 T3039 punct (,lane
R1840 T3039 T3036 parataxis lane,nerve
R1841 T3040 T3041 compound Fig.,8A
R1842 T3041 T3039 dep 8A,lane
R1843 T3042 T3039 punct ", ",lane
R1844 T3043 T3039 nummod 5,lane
R1845 T3044 T3039 punct ),lane
R1846 T3045 T3036 cc and,nerve
R1847 T3046 T3047 amod cultured,cells
R1848 T3047 T3036 conj cells,nerve
R1849 T3048 T3047 compound Schwann,cells
R1850 T3049 T3050 punct (,lane
R1851 T3050 T3047 parataxis lane,cells
R1852 T3051 T3050 nummod 6,lane
R1853 T3052 T3050 punct ),lane
R1854 T3053 T3054 advmod almost,identical
R1855 T3054 T3025 acomp identical,were
R1856 T3055 T3025 punct ", ",were
R1857 T3056 T3025 cc and,were
R1858 T3057 T3025 conj were,were
R1859 T3058 T3057 acomp shorter,were
R1860 T3059 T3058 prep than,shorter
R1861 T3060 T3059 pobj those,than
R1862 T3061 T3060 prep from,those
R1863 T3062 T3063 amod other,tissues
R1864 T3063 T3061 pobj tissues,from
R1865 T3064 T3025 punct .,were
R1866 T3066 T3067 prep In,were
R1867 T3068 T3066 pobj contrast,In
R1868 T3069 T3067 punct ", ",were
R1869 T3070 T3071 amod major,fragments
R1870 T3071 T3067 nsubj fragments,were
R1871 T3072 T3071 prep from,fragments
R1872 T3073 T3074 det the,DRG
R1873 T3074 T3072 pobj DRG,from
R1874 T3075 T3076 punct (,lane
R1875 T3076 T3071 parataxis lane,fragments
R1876 T3077 T3076 nummod 4,lane
R1877 T3078 T3076 punct ),lane
R1878 T3079 T3067 acomp longer,were
R1879 T3080 T3079 prep than,longer
R1880 T3081 T3080 pobj others,than
R1881 T3082 T3067 punct .,were
R1882 T3084 T3085 nsubj We,performed
R1883 T3086 T3085 dobj TA,performed
R1884 T3087 T3086 punct -,TA
R1885 T3088 T3086 amod cloning,TA
R1886 T3089 T3086 cc and,TA
R1887 T3090 T3086 conj sequencing,TA
R1888 T3091 T3092 aux to,analyse
R1889 T3092 T3085 advcl analyse,performed
R1890 T3093 T3094 compound exon,organization
R1891 T3094 T3092 dobj organization,analyse
R1892 T3095 T3094 prep of,organization
R1893 T3096 T3097 det the,transcripts
R1894 T3097 T3095 pobj transcripts,of
R1895 T3098 T3097 amod amplified,transcripts
R1896 T3099 T3100 punct (,8C
R1897 T3100 T3092 parataxis 8C,analyse
R1898 T3101 T3100 compound Fig.,8C
R1899 T3102 T3100 punct ),8C
R1900 T3103 T3085 punct .,performed
R1901 T3105 T3106 compound Forty,seven
R1902 T3106 T3107 nummod seven,clones
R1903 T3107 T3108 nsubjpass clones,isolated
R1904 T3109 T3108 auxpass were,isolated
R1905 T3110 T3108 prep in,isolated
R1906 T3111 T3110 amod total,in
R1907 T3112 T3108 cc and,isolated
R1908 T3113 T3114 auxpass were,classified
R1909 T3114 T3108 conj classified,isolated
R1910 T3115 T3114 prep in,classified
R1911 T3116 T3117 nummod 16,clones
R1912 T3117 T3115 pobj clones,in
R1913 T3118 T3117 amod independent,clones
R1914 T3119 T3120 punct (,G01
R1915 T3120 T3114 parataxis G01,classified
R1916 T3121 T3122 punct –,G23
R1917 T3122 T3120 prep G23,G01
R1918 T3123 T3120 punct ),G01
R1919 T3124 T3108 punct .,isolated
R1920 T3126 T3127 nsubj Comparison,revealed
R1921 T3128 T3126 prep with,Comparison
R1922 T3129 T3130 det the,sequence
R1923 T3130 T3128 pobj sequence,with
R1924 T3131 T3130 amod genomic,sequence
R1925 T3132 T3133 det the,existence
R1926 T3133 T3127 dobj existence,revealed
R1927 T3134 T3133 prep of,existence
R1928 T3135 T3136 nummod 8,exons
R1929 T3136 T3134 pobj exons,of
R1930 T3137 T3136 amod novel,exons
R1931 T3138 T3136 punct (,exons
R1932 T3139 T3140 compound exons,27S
R1933 T3140 T3136 appos 27S,exons
R1934 T3141 T3140 punct ", ",27S
R1935 T3142 T3140 conj 27L,27S
R1936 T3143 T3142 punct ", ",27L
R1937 T3144 T3142 nummod 29.1,27L
R1938 T3145 T3142 punct ", ",27L
R1939 T3146 T3142 nummod 29.3,27L
R1940 T3147 T3142 punct ", ",27L
R1941 T3148 T3142 nummod 29.5,27L
R1942 T3149 T3142 punct ", ",27L
R1943 T3150 T3142 nummod 29.7,27L
R1944 T3151 T3142 punct ", ",27L
R1945 T3152 T3142 nummod 30,27L
R1946 T3153 T3142 cc and,27L
R1947 T3154 T3142 conj 31,27L
R1948 T3155 T3127 punct ),revealed
R1949 T3156 T3127 punct .,revealed
R1950 T3158 T3159 det These,exons
R1951 T3159 T3161 nsubjpass exons,flanked
R1952 T3160 T3159 amod novel,exons
R1953 T3162 T3161 auxpass were,flanked
R1954 T3163 T3161 agent by,flanked
R1955 T3164 T3165 advmod well,defined
R1956 T3165 T3167 amod defined,introns
R1957 T3166 T3165 punct -,defined
R1958 T3167 T3163 pobj introns,by
R1959 T3168 T3169 dep that,obey
R1960 T3169 T3167 relcl obey,introns
R1961 T3170 T3171 det the,rule
R1962 T3171 T3169 dobj rule,obey
R1963 T3172 T3173 compound GT,AG
R1964 T3173 T3171 compound AG,rule
R1965 T3174 T3173 punct -,AG
R1966 T3175 T3161 punct .,flanked
R1967 T3177 T3178 compound Exon,organization
R1968 T3178 T3179 nsubjpass organization,shown
R1969 T3180 T3178 prep of,organization
R1970 T3181 T3182 det each,clone
R1971 T3182 T3180 pobj clone,of
R1972 T3183 T3179 auxpass was,shown
R1973 T3184 T3179 prep in,shown
R1974 T3185 T3186 compound Fig.,8C
R1975 T3186 T3184 pobj 8C,in
R1976 T3187 T3179 punct .,shown
R1977 T3189 T3190 amod Characteristic,feature
R1978 T3190 T3191 nsubj feature,is
R1979 T3192 T3190 prep of,feature
R1980 T3193 T3194 compound ADAM22,transcripts
R1981 T3194 T3192 pobj transcripts,of
R1982 T3195 T3196 npadvmod tissue,specific
R1983 T3196 T3197 amod specific,insertion
R1984 T3197 T3191 attr insertion,is
R1985 T3198 T3197 cc or,insertion
R1986 T3199 T3197 conj skipping,insertion
R1987 T3200 T3199 prep of,skipping
R1988 T3201 T3200 pobj exons,of
R1989 T3202 T3191 punct .,is
R1990 T3204 T3205 mark Because,is
R1991 T3205 T3223 advcl is,change
R1992 T3206 T3207 det the,number
R1993 T3207 T3205 nsubj number,is
R1994 T3208 T3207 prep of,number
R1995 T3209 T3208 pobj nucleotides,of
R1996 T3210 T3209 prep of,nucleotides
R1997 T3211 T3212 nmod exons,26
R1998 T3212 T3210 pobj 26,of
R1999 T3213 T3212 punct ", ",26
R2000 T3214 T3212 conj 27,26
R2001 T3215 T3214 punct ", ",27
R2002 T3216 T3214 conj 27L,27
R2003 T3217 T3216 punct ", ",27L
R2004 T3218 T3216 conj 29.3,27L
R2005 T3219 T3218 punct ", ",29.3
R2006 T3220 T3218 conj 29.5,29.3
R2007 T3221 T3220 cc and,29.5
R2008 T3222 T3220 conj 29.7,29.5
R2009 T3224 T3225 det a,multiple
R2010 T3225 T3205 attr multiple,is
R2011 T3226 T3225 prep of,multiple
R2012 T3227 T3226 pobj 3,of
R2013 T3228 T3223 punct ", ",change
R2014 T3229 T3223 nsubj insertion,change
R2015 T3230 T3229 cc or,insertion
R2016 T3231 T3229 conj skipping,insertion
R2017 T3232 T3229 prep of,insertion
R2018 T3233 T3234 det these,exons
R2019 T3234 T3232 pobj exons,of
R2020 T3235 T3223 aux will,change
R2021 T3236 T3223 neg not,change
R2022 T3237 T3238 det the,frame
R2023 T3238 T3223 dobj frame,change
R2024 T3239 T3238 amod downstream,frame
R2025 T3240 T3238 compound reading,frame
R2026 T3241 T3223 punct .,change
R2027 T3243 T3244 amod Known,motives
R2028 T3244 T3246 nsubjpass motives,found
R2029 T3245 T3244 compound peptide,motives
R2030 T3247 T3246 auxpass were,found
R2031 T3248 T3246 neg not,found
R2032 T3249 T3246 prep in,found
R2033 T3250 T3251 det the,sequences
R2034 T3251 T3249 pobj sequences,in
R2035 T3252 T3253 advmod newly,isolated
R2036 T3253 T3251 amod isolated,sequences
R2037 T3254 T3246 punct .,found
R2038 T3256 T3257 det This,analysis
R2039 T3257 T3261 nsubjpass analysis,summarized
R2040 T3258 T3259 compound RT,PCR
R2041 T3259 T3257 compound PCR,analysis
R2042 T3260 T3259 punct -,PCR
R2043 T3261 T3263 ccomp summarized,subdivided
R2044 T3262 T3261 auxpass is,summarized
R2045 T3264 T3265 mark as,follows
R2046 T3265 T3261 advcl follows,summarized
R2047 T3266 T3263 punct : ,subdivided
R2048 T3267 T3268 compound ADAM22,transcripts
R2049 T3268 T3263 nsubjpass transcripts,subdivided
R2050 T3269 T3263 auxpass were,subdivided
R2051 T3270 T3263 advmod roughly,subdivided
R2052 T3271 T3263 prep in,subdivided
R2053 T3272 T3273 nummod 3,groups
R2054 T3273 T3271 pobj groups,in
R2055 T3274 T3273 punct ", ",groups
R2056 T3275 T3276 compound CNS,form
R2057 T3276 T3273 appos form,groups
R2058 T3277 T3276 punct -,form
R2059 T3278 T3276 punct (,form
R2060 T3279 T3276 acl containing,form
R2061 T3280 T3279 dobj exon,containing
R2062 T3281 T3280 nummod 27,exon
R2063 T3282 T3276 punct ),form
R2064 T3283 T3276 punct ", ",form
R2065 T3284 T3285 compound DRG,form
R2066 T3285 T3276 conj form,form
R2067 T3286 T3285 punct -,form
R2068 T3287 T3285 punct (,form
R2069 T3288 T3285 acl containing,form
R2070 T3289 T3290 compound exon,27L
R2071 T3290 T3288 dobj 27L,containing
R2072 T3291 T3285 punct ),form
R2073 T3292 T3285 cc and,form
R2074 T3293 T3294 compound Schwann,cell
R2075 T3294 T3296 compound cell,form
R2076 T3295 T3294 punct -,cell
R2077 T3296 T3285 conj form,form
R2078 T3297 T3296 punct -,form
R2079 T3298 T3296 punct (,form
R2080 T3299 T3296 acl skipping,form
R2081 T3300 T3301 nmod exon,26
R2082 T3301 T3299 dobj 26,skipping
R2083 T3302 T3301 cc and,26
R2084 T3303 T3301 conj 27,26
R2085 T3304 T3263 punct ),subdivided
R2086 T3305 T3263 punct .,subdivided
R2087 T3307 T3308 prep From,concluded
R2088 T3309 T3310 det these,results
R2089 T3310 T3307 pobj results,From
R2090 T3311 T3308 punct ", ",concluded
R2091 T3312 T3308 nsubj we,concluded
R2092 T3313 T3314 mark that,expressed
R2093 T3314 T3308 ccomp expressed,concluded
R2094 T3315 T3314 nsubjpass ADAM22,expressed
R2095 T3316 T3314 auxpass is,expressed
R2096 T3317 T3314 prep in,expressed
R2097 T3318 T3319 det a,manner
R2098 T3319 T3317 pobj manner,in
R2099 T3320 T3321 compound cell,type
R2100 T3321 T3323 npadvmod type,specific
R2101 T3322 T3321 punct -,type
R2102 T3323 T3319 amod specific,manner
R2103 T3324 T3314 punct ", ",expressed
R2104 T3325 T3314 cc and,expressed
R2105 T3326 T3314 conj plays,expressed
R2106 T3327 T3328 det an,role
R2107 T3328 T3326 dobj role,plays
R2108 T3329 T3328 amod essential,role
R2109 T3330 T3326 prep in,plays
R2110 T3331 T3330 pobj myelinogenesis,in
R2111 T3332 T3326 prep in,plays
R2112 T3333 T3334 det the,PNS
R2113 T3334 T3332 pobj PNS,in
R2114 T3335 T3308 punct .,concluded
R2119 T3705 T3706 prep In,examined
R2120 T3707 T3708 det the,study
R2121 T3708 T3705 pobj study,In
R2122 T3709 T3708 amod present,study
R2123 T3710 T3706 punct ", ",examined
R2124 T3711 T3706 nsubj we,examined
R2125 T3712 T3713 det the,function
R2126 T3713 T3706 dobj function,examined
R2127 T3714 T3713 prep of,function
R2128 T3715 T3714 pobj ADAM22,of
R2129 T3716 T3706 prep by,examined
R2130 T3717 T3716 pcomp generating,by
R2131 T3718 T3717 dobj mice,generating
R2132 T3719 T3720 dep that,lacked
R2133 T3720 T3718 relcl lacked,mice
R2134 T3721 T3722 det the,gene
R2135 T3722 T3720 dobj gene,lacked
R2136 T3723 T3722 compound Adam22,gene
R2137 T3724 T3706 punct .,examined
R2138 T3726 T3727 npadvmod ADAM22,deficient
R2139 T3727 T3729 amod deficient,mice
R2140 T3728 T3727 punct -,deficient
R2141 T3729 T3730 nsubj mice,exhibited
R2142 T3731 T3732 amod severe,ataxia
R2143 T3732 T3730 dobj ataxia,exhibited
R2144 T3733 T3732 cc and,ataxia
R2145 T3734 T3735 amod premature,death
R2146 T3735 T3732 conj death,ataxia
R2147 T3736 T3730 punct .,exhibited
R2148 T3738 T3739 prep In,were
R2149 T3740 T3738 pobj contrast,In
R2150 T3741 T3739 punct ", ",were
R2151 T3742 T3743 amod heterozygous,mutants
R2152 T3743 T3739 nsubj mutants,were
R2153 T3744 T3745 amod healthy,fertile
R2154 T3745 T3739 acomp fertile,were
R2155 T3746 T3745 punct ", ",fertile
R2156 T3747 T3739 cc and,were
R2157 T3748 T3739 conj survived,were
R2158 T3749 T3750 amod more,1
R2159 T3750 T3752 nummod 1,year
R2160 T3751 T3750 quantmod than,1
R2161 T3752 T3748 npadvmod year,survived
R2162 T3753 T3748 prep without,survived
R2163 T3754 T3755 amod obvious,abnormalities
R2164 T3755 T3753 pobj abnormalities,without
R2165 T3756 T3739 punct .,were
R2166 T3758 T3759 det The,cause
R2167 T3759 T3760 nsubj cause,is
R2168 T3760 T3767 ccomp is,is
R2169 T3761 T3759 prep of,cause
R2170 T3762 T3761 pobj death,of
R2171 T3763 T3759 prep in,cause
R2172 T3764 T3765 det the,mutants
R2173 T3765 T3763 pobj mutants,in
R2174 T3766 T3765 amod homozygous,mutants
R2175 T3768 T3760 neg not,is
R2176 T3769 T3760 acomp clear,is
R2177 T3770 T3767 punct ;,is
R2178 T3771 T3767 advmod however,is
R2179 T3772 T3767 punct ", ",is
R2180 T3773 T3767 nsubj it,is
R2181 T3774 T3767 acomp likely,is
R2182 T3775 T3776 aux to,be
R2183 T3776 T3774 xcomp be,likely
R2184 T3777 T3776 prep due,be
R2185 T3778 T3777 pcomp to,due
R2186 T3779 T3780 amod multiple,seizures
R2187 T3780 T3777 pobj seizures,due
R2188 T3781 T3767 punct .,is
R2189 T3783 T3784 nsubj This,is
R2190 T3785 T3786 mark because,observed
R2191 T3786 T3784 advcl observed,is
R2192 T3787 T3788 amod convulsive,seizures
R2193 T3788 T3786 nsubjpass seizures,observed
R2194 T3789 T3786 auxpass were,observed
R2195 T3790 T3786 advmod occasionally,observed
R2196 T3791 T3786 prep in,observed
R2197 T3792 T3791 pobj homozygotes,in
R2198 T3793 T3786 punct ", ",observed
R2199 T3794 T3795 prep after,appeared
R2200 T3795 T3786 advcl appeared,observed
R2201 T3796 T3794 pobj which,after
R2202 T3797 T3798 det the,mice
R2203 T3798 T3795 nsubj mice,appeared
R2204 T3799 T3798 amod suffered,mice
R2205 T3800 T3795 oprd exhausted,appeared
R2206 T3801 T3795 cc and,appeared
R2207 T3802 T3795 punct ", ",appeared
R2208 T3803 T3804 prep in,died
R2209 T3804 T3795 conj died,appeared
R2210 T3805 T3806 amod most,cases
R2211 T3806 T3803 pobj cases,in
R2212 T3807 T3804 punct ", ",died
R2213 T3808 T3804 prep on,died
R2214 T3809 T3810 det the,day
R2215 T3810 T3808 pobj day,on
R2216 T3811 T3810 amod next,day
R2217 T3812 T3784 punct .,is
R2218 T3814 T3815 nsubjpass It,known
R2219 T3816 T3815 auxpass is,known
R2220 T3817 T3818 mark that,causes
R2221 T3818 T3815 ccomp causes,known
R2222 T3819 T3820 amod cortical,dysplasia
R2223 T3820 T3818 nsubj dysplasia,causes
R2224 T3821 T3820 acl resulting,dysplasia
R2225 T3822 T3821 prep from,resulting
R2226 T3823 T3824 amod aberrant,development
R2227 T3824 T3822 pobj development,from
R2228 T3825 T3824 compound brain,development
R2229 T3826 T3827 compound seizure,syndrome
R2230 T3827 T3818 dobj syndrome,causes
R2231 T3828 T3829 punct [,22
R2232 T3829 T3815 parataxis 22,known
R2233 T3830 T3829 punct ],22
R2234 T3831 T3815 punct .,known
R2235 T3833 T3834 prep For,exhibit
R2236 T3835 T3833 pobj example,For
R2237 T3836 T3834 punct ", ",exhibit
R2238 T3837 T3834 nsubj mice,exhibit
R2239 T3838 T3837 acl lacking,mice
R2240 T3839 T3840 det the,activator
R2241 T3840 T3838 dobj activator,lacking
R2242 T3841 T3842 npadvmod neuron,specific
R2243 T3842 T3840 amod specific,activator
R2244 T3843 T3842 punct -,specific
R2245 T3844 T3840 prep of,activator
R2246 T3845 T3844 pobj cyclin,of
R2247 T3846 T3845 nummod 5,cyclin
R2248 T3847 T3840 punct ", ",activator
R2249 T3848 T3840 appos p35,activator
R2250 T3849 T3834 punct ", ",exhibit
R2251 T3850 T3834 dobj seizures,exhibit
R2252 T3851 T3850 cc and,seizures
R2253 T3852 T3853 det a,defect
R2254 T3853 T3850 conj defect,seizures
R2255 T3854 T3853 amod severe,defect
R2256 T3855 T3853 amod neuronal,defect
R2257 T3856 T3853 compound migration,defect
R2258 T3857 T3858 punct [,23
R2259 T3858 T3834 parataxis 23,exhibit
R2260 T3859 T3858 punct ],23
R2261 T3860 T3834 punct .,exhibit
R2262 T3862 T3863 mark Because,expressed
R2263 T3863 T3867 advcl expressed,hypothesized
R2264 T3864 T3865 compound ADAM22,protein
R2265 T3865 T3863 nsubjpass protein,expressed
R2266 T3866 T3863 auxpass is,expressed
R2267 T3868 T3863 prep on,expressed
R2268 T3869 T3870 det the,surface
R2269 T3870 T3868 pobj surface,on
R2270 T3871 T3870 compound cell,surface
R2271 T3872 T3863 cc and,expressed
R2272 T3873 T3863 conj is,expressed
R2273 T3874 T3873 acomp likely,is
R2274 T3875 T3876 aux to,involved
R2275 T3876 T3874 xcomp involved,likely
R2276 T3877 T3876 auxpass be,involved
R2277 T3878 T3876 prep in,involved
R2278 T3879 T3880 nmod cell,cell
R2279 T3880 T3882 nmod cell,interactions
R2280 T3881 T3880 punct -,cell
R2281 T3882 T3878 pobj interactions,in
R2282 T3883 T3880 cc or,cell
R2283 T3884 T3885 compound cell,matrix
R2284 T3885 T3880 conj matrix,cell
R2285 T3886 T3885 punct -,matrix
R2286 T3887 T3867 punct ", ",hypothesized
R2287 T3888 T3867 nsubj we,hypothesized
R2288 T3889 T3890 mark that,generate
R2289 T3890 T3867 ccomp generate,hypothesized
R2290 T3891 T3890 nsubj depletion,generate
R2291 T3892 T3891 prep of,depletion
R2292 T3893 T3892 pobj ADAM22,of
R2293 T3894 T3890 aux would,generate
R2294 T3895 T3896 amod aberrant,migration
R2295 T3896 T3890 dobj migration,generate
R2296 T3897 T3896 amod neuronal,migration
R2297 T3898 T3867 punct .,hypothesized
R2298 T3900 T3901 amod Histochemical,analysis
R2299 T3901 T3902 nsubjpass analysis,performed
R2300 T3903 T3902 auxpass was,performed
R2301 T3904 T3905 aux to,verify
R2302 T3905 T3902 advcl verify,performed
R2303 T3906 T3907 det this,hypothesis
R2304 T3907 T3905 dobj hypothesis,verify
R2305 T3908 T3902 punct .,performed
R2306 T3910 T3911 advmod However,observed
R2307 T3912 T3911 punct ", ",observed
R2308 T3913 T3914 det no,abnormalities
R2309 T3914 T3911 nsubjpass abnormalities,observed
R2310 T3915 T3914 amod marked,abnormalities
R2311 T3916 T3911 auxpass were,observed
R2312 T3917 T3911 prep in,observed
R2313 T3918 T3919 det the,brain
R2314 T3919 T3917 pobj brain,in
R2315 T3920 T3919 compound mutant,brain
R2316 T3921 T3919 compound mouse,brain
R2317 T3922 T3911 punct ", ",observed
R2318 T3923 T3911 advcl ruling,observed
R2319 T3924 T3923 prt out,ruling
R2320 T3925 T3926 det a,role
R2321 T3926 T3923 dobj role,ruling
R2322 T3927 T3926 amod critical,role
R2323 T3928 T3926 prep for,role
R2324 T3929 T3928 pobj ADAM22,for
R2325 T3930 T3926 prep in,role
R2326 T3931 T3932 amod neuronal,migration
R2327 T3932 T3930 pobj migration,in
R2328 T3933 T3911 punct .,observed
R2329 T3935 T3936 det Another,explanation
R2330 T3936 T3938 nsubj explanation,is
R2331 T3937 T3936 amod possible,explanation
R2332 T3939 T3940 mark that,caused
R2333 T3940 T3938 ccomp caused,is
R2334 T3941 T3942 det the,death
R2335 T3942 T3940 nsubjpass death,caused
R2336 T3943 T3942 amod premature,death
R2337 T3944 T3942 prep in,death
R2338 T3945 T3946 amod first,weeks
R2339 T3946 T3944 pobj weeks,in
R2340 T3947 T3946 nummod 2,weeks
R2341 T3948 T3946 prep of,weeks
R2342 T3949 T3948 pobj life,of
R2343 T3950 T3940 auxpass is,caused
R2344 T3951 T3940 agent by,caused
R2345 T3952 T3953 det the,dysfunction
R2346 T3953 T3951 pobj dysfunction,by
R2347 T3954 T3953 prep of,dysfunction
R2348 T3955 T3956 det the,system
R2349 T3956 T3954 pobj system,of
R2350 T3957 T3956 amod autonomic,system
R2351 T3958 T3956 amod nervous,system
R2352 T3959 T3938 punct .,is
R2353 T3961 T3962 advmod Especially,be
R2354 T3963 T3962 punct ", ",be
R2355 T3964 T3962 nsubj nerves,be
R2356 T3965 T3966 dep which,control
R2357 T3966 T3964 relcl control,nerves
R2358 T3967 T3968 det the,breathing
R2359 T3968 T3966 dobj breathing,control
R2360 T3969 T3962 aux would,be
R2361 T3970 T3971 advmod very,important
R2362 T3971 T3962 acomp important,be
R2363 T3972 T3962 punct ", ",be
R2364 T3973 T3974 mark because,undergoes
R2365 T3974 T3962 advcl undergoes,be
R2366 T3975 T3976 det the,system
R2367 T3976 T3974 nsubj system,undergoes
R2368 T3977 T3976 compound respiration,system
R2369 T3978 T3979 amod significant,maturation
R2370 T3979 T3974 dobj maturation,undergoes
R2371 T3980 T3974 prep in,undergoes
R2372 T3981 T3982 det the,weeks
R2373 T3982 T3980 pobj weeks,in
R2374 T3983 T3982 amod first,weeks
R2375 T3984 T3985 quantmod 2,3
R2376 T3985 T3982 nummod 3,weeks
R2377 T3986 T3985 punct –,3
R2378 T3987 T3982 prep after,weeks
R2379 T3988 T3987 pobj birth,after
R2380 T3989 T3962 punct .,be
R2381 T3991 T3992 prep For,exhibits
R2382 T3993 T3991 pobj example,For
R2383 T3994 T3992 punct ", ",exhibits
R2384 T3995 T3996 det the,rat
R2385 T3996 T3992 nsubj rat,exhibits
R2386 T3997 T3998 npadvmod myelin,deficient
R2387 T3998 T3996 amod deficient,rat
R2388 T3999 T3998 punct -,deficient
R2389 T4000 T4001 punct (,MD
R2390 T4001 T3998 parataxis MD,deficient
R2391 T4002 T4001 punct ),MD
R2392 T4003 T3996 punct ", ",rat
R2393 T4004 T4005 dep which,carries
R2394 T4005 T3996 relcl carries,rat
R2395 T4006 T4007 det a,mutation
R2396 T4007 T4005 dobj mutation,carries
R2397 T4008 T4007 compound point,mutation
R2398 T4009 T4005 prep in,carries
R2399 T4010 T4011 det the,gene
R2400 T4011 T4009 pobj gene,in
R2401 T4012 T4013 nmod proteolipid,protein
R2402 T4013 T4011 nmod protein,gene
R2403 T4014 T4013 punct (,protein
R2404 T4015 T4013 appos PLP,protein
R2405 T4016 T4011 punct ),gene
R2406 T4017 T3992 punct ", ",exhibits
R2407 T4018 T4019 nmod ataxia,dysmyelination
R2408 T4019 T3992 dobj dysmyelination,exhibits
R2409 T4020 T4019 punct ", ",dysmyelination
R2410 T4021 T4019 nmod tremor,dysmyelination
R2411 T4022 T4019 punct ", ",dysmyelination
R2412 T4023 T3992 cc and,exhibits
R2413 T4024 T3992 conj dies,exhibits
R2414 T4025 T4024 prep at,dies
R2415 T4026 T4027 advmod approximately,day
R2416 T4027 T4025 pobj day,at
R2417 T4028 T4027 amod postnatal,day
R2418 T4029 T4027 nummod 21,day
R2419 T4030 T3992 punct .,exhibits
R2420 T4032 T4033 prep In,caused
R2421 T4034 T4035 compound MD,rat
R2422 T4035 T4032 pobj rat,In
R2423 T4036 T4033 punct ", ",caused
R2424 T4037 T4038 amod early,death
R2425 T4038 T4033 nsubjpass death,caused
R2426 T4039 T4033 auxpass is,caused
R2427 T4040 T4033 agent by,caused
R2428 T4041 T4042 det the,dysfunction
R2429 T4042 T4040 pobj dysfunction,by
R2430 T4043 T4042 prep of,dysfunction
R2431 T4044 T4045 det the,stem
R2432 T4045 T4043 pobj stem,of
R2433 T4046 T4045 compound brain,stem
R2434 T4047 T4048 dep which,is
R2435 T4048 T4045 relcl is,stem
R2436 T4049 T4048 acomp essential,is
R2437 T4050 T4049 prep for,essential
R2438 T4051 T4052 amod autonomic,control
R2439 T4052 T4050 pobj control,for
R2440 T4053 T4052 prep of,control
R2441 T4054 T4053 pobj respiration,of
R2442 T4055 T4052 prep during,control
R2443 T4056 T4055 pobj hypoxia,during
R2444 T4057 T4058 punct [,24
R2445 T4058 T4033 parataxis 24,caused
R2446 T4059 T4058 punct ],24
R2447 T4060 T4033 punct .,caused
R2448 T4062 T4063 amod Further,study
R2449 T4063 T4064 nsubjpass study,needed
R2450 T4065 T4064 auxpass is,needed
R2451 T4066 T4067 aux to,verify
R2452 T4067 T4064 advcl verify,needed
R2453 T4068 T4069 det the,cause
R2454 T4069 T4067 dobj cause,verify
R2455 T4070 T4069 prep of,cause
R2456 T4071 T4070 pobj death,of
R2457 T4072 T4069 prep in,cause
R2458 T4073 T4074 npadvmod ADAM22,deficient
R2459 T4074 T4076 amod deficient,mice
R2460 T4075 T4074 punct -,deficient
R2461 T4076 T4072 pobj mice,in
R2462 T4077 T4064 punct .,needed
R2463 T4079 T4080 det Another,phenotype
R2464 T4080 T4082 nsubj phenotype,was
R2465 T4081 T4080 amod interesting,phenotype
R2466 T4083 T4080 acl observed,phenotype
R2467 T4084 T4083 prep in,observed
R2468 T4085 T4086 npadvmod ADAM22,deficient
R2469 T4086 T4088 amod deficient,mice
R2470 T4087 T4086 punct -,deficient
R2471 T4088 T4084 pobj mice,in
R2472 T4089 T4082 attr hypomyelinogenesis,was
R2473 T4090 T4082 punct .,was
R2474 T4092 T4093 amod Histopathological,analysis
R2475 T4093 T4094 nsubj analysis,revealed
R2476 T4095 T4096 amod marked,hypomyelination
R2477 T4096 T4094 dobj hypomyelination,revealed
R2478 T4097 T4094 prep in,revealed
R2479 T4098 T4099 det the,nerves
R2480 T4099 T4097 pobj nerves,in
R2481 T4100 T4099 amod sciatic,nerves
R2482 T4101 T4100 cc and,sciatic
R2483 T4102 T4100 conj trigeminal,sciatic
R2484 T4103 T4094 punct .,revealed
R2485 T4105 T4106 advmod However,was
R2486 T4107 T4106 punct ", ",was
R2487 T4108 T4109 compound myelin,formation
R2488 T4109 T4106 nsubj formation,was
R2489 T4110 T4109 prep in,formation
R2490 T4111 T4112 det the,cord
R2491 T4112 T4110 pobj cord,in
R2492 T4113 T4112 amod spinal,cord
R2493 T4114 T4112 cc and,cord
R2494 T4115 T4116 det the,brain
R2495 T4116 T4112 conj brain,cord
R2496 T4117 T4106 acomp normal,was
R2497 T4118 T4106 punct .,was
R2498 T4120 T4121 nsubj It,was
R2499 T4122 T4121 advmod therefore,was
R2500 T4123 T4121 acomp evident,was
R2501 T4124 T4125 mark that,occurred
R2502 T4125 T4121 ccomp occurred,was
R2503 T4126 T4125 nsubj hypomyelinogenesis,occurred
R2504 T4127 T4128 advmod specifically,in
R2505 T4128 T4125 prep in,occurred
R2506 T4129 T4130 det the,nerves
R2507 T4130 T4128 pobj nerves,in
R2508 T4131 T4130 amod peripheral,nerves
R2509 T4132 T4125 prep in,occurred
R2510 T4133 T4134 det the,mice
R2511 T4134 T4132 pobj mice,in
R2512 T4135 T4134 compound knockout,mice
R2513 T4136 T4125 punct ", ",occurred
R2514 T4137 T4125 advcl suggesting,occurred
R2515 T4138 T4139 compound Schwann,cell
R2516 T4139 T4140 compound cell,dysfunction
R2517 T4140 T4137 dobj dysfunction,suggesting
R2518 T4141 T4121 punct .,was
R2519 T4143 T4144 prep In,was
R2520 T4145 T4146 det the,nerve
R2521 T4146 T4143 pobj nerve,In
R2522 T4147 T4146 nmod mutant,nerve
R2523 T4148 T4146 amod sciatic,nerve
R2524 T4149 T4144 punct ", ",was
R2525 T4150 T4151 det the,density
R2526 T4151 T4144 nsubj density,was
R2527 T4152 T4151 prep of,density
R2528 T4153 T4154 amod pro-myelinating,cells
R2529 T4154 T4152 pobj cells,of
R2530 T4155 T4154 compound Schwann,cells
R2531 T4156 T4157 punct (,negative
R2532 T4157 T4154 parataxis negative,cells
R2533 T4158 T4159 npadvmod S100,positive
R2534 T4159 T4157 amod positive,negative
R2535 T4160 T4159 punct -,positive
R2536 T4161 T4157 punct ", ",negative
R2537 T4162 T4157 npadvmod MBP,negative
R2538 T4163 T4157 punct -,negative
R2539 T4164 T4157 punct ),negative
R2540 T4165 T4166 advmod markedly,higher
R2541 T4166 T4144 acomp higher,was
R2542 T4167 T4166 prep than,higher
R2543 T4168 T4167 pobj that,than
R2544 T4169 T4168 acl seen,that
R2545 T4170 T4169 prep in,seen
R2546 T4171 T4170 pobj heterozygotes,in
R2547 T4172 T4144 punct .,was
R2548 T4174 T4175 amod Higher,density
R2549 T4175 T4179 nsubj density,indicate
R2550 T4176 T4177 amod pro-myelinating,cell
R2551 T4177 T4175 compound cell,density
R2552 T4178 T4177 compound Schwann,cell
R2553 T4180 T4175 cc and,density
R2554 T4181 T4182 amod marked,delay
R2555 T4182 T4175 conj delay,density
R2556 T4183 T4182 prep in,delay
R2557 T4184 T4185 compound myelin,formation
R2558 T4185 T4183 pobj formation,in
R2559 T4186 T4187 mark that,plays
R2560 T4187 T4179 ccomp plays,indicate
R2561 T4188 T4187 nsubj ADAM22,plays
R2562 T4189 T4190 det an,role
R2563 T4190 T4187 dobj role,plays
R2564 T4191 T4190 amod important,role
R2565 T4192 T4187 prep in,plays
R2566 T4193 T4194 compound Schwann,cell
R2567 T4194 T4195 compound cell,differentiation
R2568 T4195 T4192 pobj differentiation,in
R2569 T4196 T4192 punct ", ",in
R2570 T4197 T4192 cc but,in
R2571 T4198 T4197 neg not,but
R2572 T4199 T4192 conj in,in
R2573 T4200 T4199 pobj proliferation,in
R2574 T4201 T4179 punct .,indicate
R2575 T4203 T4204 nsubj Investigations,centre
R2576 T4205 T4203 prep on,Investigations
R2577 T4206 T4207 advmod why,occurred
R2578 T4207 T4205 pcomp occurred,on
R2579 T4208 T4207 nsubj hypomyelinogenesis,occurred
R2580 T4209 T4207 prep in,occurred
R2581 T4210 T4211 npadvmod ADAM22,deficient
R2582 T4211 T4213 amod deficient,mice
R2583 T4212 T4211 punct -,deficient
R2584 T4213 T4209 pobj mice,in
R2585 T4214 T4204 prep on,centre
R2586 T4215 T4214 pobj integrin,on
R2587 T4216 T4204 punct .,centre
R2588 T4218 T4219 nsubj This,is
R2589 T4220 T4221 mark because,shown
R2590 T4221 T4219 advcl shown,is
R2591 T4222 T4223 amod several,ADAMs
R2592 T4223 T4221 nsubjpass ADAMs,shown
R2593 T4224 T4221 aux have,shown
R2594 T4225 T4221 auxpass been,shown
R2595 T4226 T4227 aux to,interact
R2596 T4227 T4221 xcomp interact,shown
R2597 T4228 T4227 prep with,interact
R2598 T4229 T4228 pobj integrins,with
R2599 T4230 T4227 prep via,interact
R2600 T4231 T4232 compound disintegrin,domains
R2601 T4232 T4230 pobj domains,via
R2602 T4233 T4234 punct [,9
R2603 T4234 T4219 parataxis 9,is
R2604 T4235 T4234 punct ],9
R2605 T4236 T4219 punct ", ",is
R2606 T4237 T4219 cc and,is
R2607 T4238 T4239 nsubjpass it,suggested
R2608 T4239 T4219 conj suggested,is
R2609 T4240 T4239 aux has,suggested
R2610 T4241 T4239 auxpass been,suggested
R2611 T4242 T4243 mark that,plays
R2612 T4243 T4239 ccomp plays,suggested
R2613 T4244 T4245 nmod alpha,integrin
R2614 T4245 T4243 nsubj integrin,plays
R2615 T4246 T4244 punct -,alpha
R2616 T4247 T4244 nummod 6,alpha
R2617 T4248 T4245 nmod beta,integrin
R2618 T4249 T4248 punct -,beta
R2619 T4250 T4248 nummod 1,beta
R2620 T4251 T4245 prep on,integrin
R2621 T4252 T4253 det the,cell
R2622 T4253 T4251 pobj cell,on
R2623 T4254 T4253 compound Schwann,cell
R2624 T4255 T4256 det an,role
R2625 T4256 T4243 dobj role,plays
R2626 T4257 T4256 amod important,role
R2627 T4258 T4243 prep in,plays
R2628 T4259 T4260 compound myelin,formation
R2629 T4260 T4258 pobj formation,in
R2630 T4261 T4262 punct [,25
R2631 T4262 T4239 parataxis 25,suggested
R2632 T4263 T4262 punct ],25
R2633 T4264 T4239 punct .,suggested
R2634 T4266 T4267 prep In,is
R2635 T4268 T4266 pobj addition,In
R2636 T4269 T4267 punct ", ",is
R2637 T4270 T4267 nsubj laminin,is
R2638 T4271 T4270 punct -,laminin
R2639 T4272 T4270 nummod 2,laminin
R2640 T4273 T4274 det a,player
R2641 T4274 T4267 attr player,is
R2642 T4275 T4274 amod key,player
R2643 T4276 T4274 prep in,player
R2644 T4277 T4278 amod peripheral,formation
R2645 T4278 T4276 pobj formation,in
R2646 T4279 T4278 compound myelin,formation
R2647 T4280 T4267 punct ", ",is
R2648 T4281 T4282 mark because,causes
R2649 T4282 T4267 advcl causes,is
R2650 T4283 T4284 nmod laminin,deficiency
R2651 T4284 T4282 nsubj deficiency,causes
R2652 T4285 T4283 punct -,laminin
R2653 T4286 T4283 nummod 2,laminin
R2654 T4287 T4282 dobj dysmyelination,causes
R2655 T4288 T4282 prep in,causes
R2656 T4289 T4288 pobj mice,in
R2657 T4290 T4289 cc and,mice
R2658 T4291 T4289 conj humans,mice
R2659 T4292 T4282 punct ", ",causes
R2660 T4293 T4282 cc and,causes
R2661 T4294 T4295 poss its,receptors
R2662 T4295 T4296 nsubjpass receptors,shown
R2663 T4296 T4282 conj shown,causes
R2664 T4297 T4295 punct ", ",receptors
R2665 T4298 T4299 nmod alpha,integrin
R2666 T4299 T4295 appos integrin,receptors
R2667 T4300 T4298 punct -,alpha
R2668 T4301 T4298 nummod 6,alpha
R2669 T4302 T4299 nmod beta,integrin
R2670 T4303 T4302 punct -,beta
R2671 T4304 T4302 nummod 1,beta
R2672 T4305 T4299 cc and,integrin
R2673 T4306 T4299 conj dystroglycan,integrin
R2674 T4307 T4296 punct ", ",shown
R2675 T4308 T4296 auxpass are,shown
R2676 T4309 T4296 advmod also,shown
R2677 T4310 T4311 aux to,involved
R2678 T4311 T4296 xcomp involved,shown
R2679 T4312 T4311 auxpass be,involved
R2680 T4313 T4311 advmod deeply,involved
R2681 T4314 T4311 prep in,involved
R2682 T4315 T4316 compound myelin,formation
R2683 T4316 T4314 pobj formation,in
R2684 T4317 T4318 punct [,27
R2685 T4318 T4267 parataxis 27,is
R2686 T4319 T4318 nummod 26,27
R2687 T4320 T4318 punct ",",27
R2688 T4321 T4318 punct ],27
R2689 T4322 T4267 punct .,is
R2690 T4324 T4325 det The,studies
R2691 T4325 T4328 nsubj studies,suggested
R2692 T4326 T4327 amod conditional,knockout
R2693 T4327 T4325 compound knockout,studies
R2694 T4329 T4325 prep of,studies
R2695 T4330 T4331 nmod beta,integrin
R2696 T4331 T4329 pobj integrin,of
R2697 T4332 T4330 punct -,beta
R2698 T4333 T4330 nummod 1,beta
R2699 T4334 T4331 cc and,integrin
R2700 T4335 T4336 compound laminin,gamma
R2701 T4336 T4331 conj gamma,integrin
R2702 T4337 T4336 punct -,gamma
R2703 T4338 T4336 nummod 1,gamma
R2704 T4339 T4325 prep in,studies
R2705 T4340 T4341 compound Schwann,cell
R2706 T4341 T4339 pobj cell,in
R2707 T4342 T4343 mark that,are
R2708 T4343 T4328 ccomp are,suggested
R2709 T4344 T4345 preconj both,laminin
R2710 T4345 T4343 nsubj laminin,are
R2711 T4346 T4345 punct -,laminin
R2712 T4347 T4345 nummod 2,laminin
R2713 T4348 T4345 cc and,laminin
R2714 T4349 T4345 conj integrins,laminin
R2715 T4350 T4343 acomp essential,are
R2716 T4351 T4350 prep for,essential
R2717 T4352 T4351 pobj segregation,for
R2718 T4353 T4352 prep of,segregation
R2719 T4354 T4355 amod large,bundles
R2720 T4355 T4353 pobj bundles,of
R2721 T4356 T4355 prep of,bundles
R2722 T4357 T4356 pobj axons,of
R2723 T4358 T4352 prep in,segregation
R2724 T4359 T4360 det the,stage
R2725 T4360 T4358 pobj stage,in
R2726 T4361 T4360 amod early,stage
R2727 T4362 T4360 prep of,stage
R2728 T4363 T4364 compound myelin,formation
R2729 T4364 T4362 pobj formation,of
R2730 T4365 T4366 punct [,28
R2731 T4366 T4328 parataxis 28,suggested
R2732 T4367 T4366 nummod 25,28
R2733 T4368 T4366 punct ",",28
R2734 T4369 T4366 punct ],28
R2735 T4370 T4328 punct .,suggested
R2736 T4372 T4373 prep In,was
R2737 T4373 T4385 ccomp was,seen
R2738 T4374 T4372 pobj contrast,In
R2739 T4375 T4373 punct ", ",was
R2740 T4376 T4377 det the,phenotype
R2741 T4377 T4373 nsubj phenotype,was
R2742 T4378 T4377 acl seen,phenotype
R2743 T4379 T4378 prep in,seen
R2744 T4380 T4381 det the,mice
R2745 T4381 T4379 pobj mice,in
R2746 T4382 T4383 npadvmod ADAM22,deficient
R2747 T4383 T4381 amod deficient,mice
R2748 T4384 T4383 punct -,deficient
R2749 T4386 T4387 advmod quite,different
R2750 T4387 T4373 acomp different,was
R2751 T4388 T4385 punct : ,seen
R2752 T4389 T4385 prep for,seen
R2753 T4390 T4389 pobj example,for
R2754 T4391 T4385 punct ", ",seen
R2755 T4392 T4393 amod unsorted,bundles
R2756 T4393 T4385 nsubjpass bundles,seen
R2757 T4394 T4393 prep of,bundles
R2758 T4395 T4394 pobj axons,of
R2759 T4396 T4385 auxpass were,seen
R2760 T4397 T4385 neg not,seen
R2761 T4398 T4385 prep in,seen
R2762 T4399 T4398 pobj nerves,in
R2763 T4400 T4399 cc and,nerves
R2764 T4401 T4399 conj roots,nerves
R2765 T4402 T4385 punct .,seen
R2766 T4404 T4405 nsubj We,assume
R2767 T4406 T4407 mark that,modulates
R2768 T4407 T4405 ccomp modulates,assume
R2769 T4408 T4407 nsubj ADAM22,modulates
R2770 T4409 T4407 advmod partially,modulates
R2771 T4410 T4411 npadvmod integrin,mediated
R2772 T4411 T4413 amod mediated,signals
R2773 T4412 T4411 punct -,mediated
R2774 T4413 T4407 dobj signals,modulates
R2775 T4414 T4407 cc or,modulates
R2776 T4415 T4416 auxpass is,involved
R2777 T4416 T4407 conj involved,modulates
R2778 T4417 T4416 prep at,involved
R2779 T4418 T4419 det a,stage
R2780 T4419 T4417 pobj stage,at
R2781 T4420 T4419 amod later,stage
R2782 T4421 T4419 prep of,stage
R2783 T4422 T4423 compound myelin,formation
R2784 T4423 T4421 pobj formation,of
R2785 T4424 T4405 punct .,assume
R2786 T4470 T4471 prep In,generated
R2787 T4472 T4470 pobj summary,In
R2788 T4473 T4471 punct ", ",generated
R2789 T4474 T4471 nsubj we,generated
R2790 T4475 T4476 npadvmod ADAM22,deficient
R2791 T4476 T4478 amod deficient,mice
R2792 T4477 T4476 punct -,deficient
R2793 T4478 T4471 dobj mice,generated
R2794 T4479 T4471 cc and,generated
R2795 T4480 T4471 conj proved,generated
R2796 T4481 T4482 mark that,plays
R2797 T4482 T4480 ccomp plays,proved
R2798 T4483 T4482 nsubj ADAM22,plays
R2799 T4484 T4485 det an,role
R2800 T4485 T4482 dobj role,plays
R2801 T4486 T4485 amod essential,role
R2802 T4487 T4482 prep in,plays
R2803 T4488 T4489 preconj both,CNS
R2804 T4489 T4487 pobj CNS,in
R2805 T4490 T4489 det the,CNS
R2806 T4491 T4489 cc and,CNS
R2807 T4492 T4493 det the,PNS
R2808 T4493 T4489 conj PNS,CNS
R2809 T4494 T4471 punct .,generated
R2810 T4496 T4497 det The,results
R2811 T4497 T4498 nsubj results,suggest
R2812 T4499 T4497 prep of,results
R2813 T4500 T4501 det this,study
R2814 T4501 T4499 pobj study,of
R2815 T4502 T4503 mark that,involved
R2816 T4503 T4498 ccomp involved,suggest
R2817 T4504 T4503 nsubjpass ADAM22,involved
R2818 T4505 T4503 auxpass is,involved
R2819 T4506 T4503 prep in,involved
R2820 T4507 T4508 amod human,diseases
R2821 T4508 T4506 pobj diseases,in
R2822 T4509 T4510 amod such,as
R2823 T4510 T4508 prep as,diseases
R2824 T4511 T4510 pobj epilepsy,as
R2825 T4512 T4511 cc and,epilepsy
R2826 T4513 T4514 amod peripheral,neuropathy
R2827 T4514 T4511 conj neuropathy,epilepsy
R2828 T4515 T4498 punct .,suggest
R2829 T4517 T4518 det The,gene
R2830 T4518 T4521 nsubj gene,is
R2831 T4519 T4518 amod human,gene
R2832 T4520 T4518 compound ADAM22,gene
R2833 T4522 T4523 advmod relatively,large
R2834 T4523 T4521 acomp large,is
R2835 T4524 T4521 punct ", ",is
R2836 T4525 T4521 prep at,is
R2837 T4526 T4527 nummod 300,kb
R2838 T4527 T4525 pobj kb,at
R2839 T4528 T4527 prep in,kb
R2840 T4529 T4528 pobj length,in
R2841 T4530 T4521 punct ", ",is
R2842 T4531 T4521 cc and,is
R2843 T4532 T4533 auxpass is,comprised
R2844 T4533 T4521 conj comprised,is
R2845 T4534 T4533 prep of,comprised
R2846 T4535 T4536 amod more,30
R2847 T4536 T4538 nummod 30,exons
R2848 T4537 T4536 quantmod than,30
R2849 T4538 T4534 pobj exons,of
R2850 T4539 T4521 punct .,is
R2851 T4541 T4542 nsubjpass It,assigned
R2852 T4543 T4542 auxpass is,assigned
R2853 T4544 T4542 prep on,assigned
R2854 T4545 T4544 pobj 7q21,on
R2855 T4546 T4545 punct ", ",7q21
R2856 T4547 T4548 advmod where,identified
R2857 T4548 T4545 relcl identified,7q21
R2858 T4549 T4550 amod several,aberrations
R2859 T4550 T4548 nsubjpass aberrations,identified
R2860 T4551 T4550 amod chromosomal,aberrations
R2861 T4552 T4553 dep that,accompanied
R2862 T4553 T4550 relcl accompanied,aberrations
R2863 T4554 T4553 auxpass are,accompanied
R2864 T4555 T4553 agent by,accompanied
R2865 T4556 T4557 amod neurological,disorders
R2866 T4557 T4555 pobj disorders,by
R2867 T4558 T4548 aux have,identified
R2868 T4559 T4548 auxpass been,identified
R2869 T4560 T4561 punct [,30
R2870 T4561 T4542 parataxis 30,assigned
R2871 T4562 T4561 nummod 29,30
R2872 T4563 T4561 punct ",",30
R2873 T4564 T4561 punct ],30
R2874 T4565 T4542 punct .,assigned
R2875 T4567 T4568 amod Further,analysis
R2876 T4568 T4572 nsubj analysis,lead
R2877 T4569 T4568 amod detailed,analysis
R2878 T4570 T4571 amod molecular,function
R2879 T4571 T4568 compound function,analysis
R2880 T4573 T4568 prep of,analysis
R2881 T4574 T4573 pobj ADAM22,of
R2882 T4575 T4572 aux may,lead
R2883 T4576 T4572 prep to,lead
R2884 T4577 T4576 pobj progress,to
R2885 T4578 T4577 prep in,progress
R2886 T4579 T4578 pcomp uncovering,in
R2887 T4580 T4581 det the,mechanisms
R2888 T4581 T4579 dobj mechanisms,uncovering
R2889 T4582 T4583 dep that,underlie
R2890 T4583 T4581 relcl underlie,mechanisms
R2891 T4584 T4585 amod certain,diseases
R2892 T4585 T4583 dobj diseases,underlie
R2893 T4586 T4585 amod human,diseases
R2894 T4587 T4585 amod neurological,diseases
R2895 T4588 T4572 punct .,lead
R2896 T4652 T4651 prep of,Construction
R2897 T4653 T4654 det an,vector
R2898 T4654 T4652 pobj vector,of
R2899 T4655 T4654 nmod Adam22,vector
R2900 T4656 T4657 npadvmod gene,targeting
R2901 T4657 T4654 amod targeting,vector
R2902 T4658 T4657 punct -,targeting
R2903 T4660 T4661 det The,fragments
R2904 T4661 T4667 nsubjpass fragments,amplified
R2905 T4662 T4663 nummod 18,kb
R2906 T4663 T4661 nmod kb,fragments
R2907 T4664 T4663 punct -,kb
R2908 T4665 T4661 amod genomic,fragments
R2909 T4666 T4661 compound DNA,fragments
R2910 T4668 T4661 acl covering,fragments
R2911 T4669 T4670 nmod exons,5
R2912 T4670 T4668 dobj 5,covering
R2913 T4671 T4670 punct ", ",5
R2914 T4672 T4670 conj 6,5
R2915 T4673 T4672 punct ", ",6
R2916 T4674 T4672 conj 7,6
R2917 T4675 T4674 punct ", ",7
R2918 T4676 T4674 conj 8,7
R2919 T4677 T4676 cc and,8
R2920 T4678 T4676 conj 9,8
R2921 T4679 T4670 prep of,5
R2922 T4680 T4681 det the,gene
R2923 T4681 T4679 pobj gene,of
R2924 T4682 T4681 compound Adam22,gene
R2925 T4683 T4667 auxpass were,amplified
R2926 T4684 T4667 prep from,amplified
R2927 T4685 T4686 nmod C57BL,DNA
R2928 T4686 T4684 pobj DNA,from
R2929 T4687 T4685 punct /,C57BL
R2930 T4688 T4685 nummod 6,C57BL
R2931 T4689 T4686 amod genomic,DNA
R2932 T4690 T4667 prep by,amplified
R2933 T4691 T4690 pobj PCR,by
R2934 T4692 T4667 advcl using,amplified
R2935 T4693 T4694 compound Expand,mix
R2936 T4694 T4692 dobj mix,using
R2937 T4695 T4696 compound Hi,Fidelity
R2938 T4696 T4694 compound Fidelity,mix
R2939 T4697 T4696 punct -,Fidelity
R2940 T4698 T4694 compound enzyme,mix
R2941 T4699 T4700 punct (,Diagnostics
R2942 T4700 T4694 parataxis Diagnostics,mix
R2943 T4701 T4700 compound Roche,Diagnostics
R2944 T4702 T4700 punct ),Diagnostics
R2945 T4703 T4694 cc and,mix
R2946 T4704 T4694 conj primers,mix
R2947 T4705 T4704 acl designed,primers
R2948 T4706 T4705 prep for,designed
R2949 T4707 T4708 det each,exon
R2950 T4708 T4706 pobj exon,for
R2951 T4709 T4667 punct .,amplified
R2952 T4711 T4712 aux To,generate
R2953 T4712 T4713 advcl generate,inserted
R2954 T4714 T4715 det a,mutation
R2955 T4715 T4712 dobj mutation,generate
R2956 T4716 T4712 prep in,generate
R2957 T4717 T4718 det the,gene
R2958 T4718 T4716 pobj gene,in
R2959 T4719 T4718 compound mouse,gene
R2960 T4720 T4718 compound Adam22,gene
R2961 T4721 T4713 punct ", ",inserted
R2962 T4722 T4713 nsubj we,inserted
R2963 T4723 T4724 det the,cassette
R2964 T4724 T4713 dobj cassette,inserted
R2965 T4725 T4724 compound PGKneo,cassette
R2966 T4726 T4713 prep into,inserted
R2967 T4727 T4726 pobj exon,into
R2968 T4728 T4727 nummod 8,exon
R2969 T4729 T4727 prep of,exon
R2970 T4730 T4731 det the,gene
R2971 T4731 T4729 pobj gene,of
R2972 T4732 T4731 compound Adam22,gene
R2973 T4733 T4713 punct .,inserted
R2974 T4735 T4736 det The,construct
R2975 T4736 T4739 nsubj construct,consisted
R2976 T4737 T4736 amod final,construct
R2977 T4738 T4736 compound targeting,construct
R2978 T4740 T4739 prep of,consisted
R2979 T4741 T4742 det a,fragment
R2980 T4742 T4740 pobj fragment,of
R2981 T4743 T4744 nummod 10.5,kb
R2982 T4744 T4742 nmod kb,fragment
R2983 T4745 T4744 punct -,kb
R2984 T4746 T4747 amod genomic,DNA
R2985 T4747 T4742 compound DNA,fragment
R2986 T4748 T4749 dep that,interrupted
R2987 T4749 T4742 relcl interrupted,fragment
R2988 T4750 T4749 auxpass was,interrupted
R2989 T4751 T4749 agent by,interrupted
R2990 T4752 T4753 det the,insertion
R2991 T4753 T4751 pobj insertion,by
R2992 T4754 T4753 prep of,insertion
R2993 T4755 T4756 det the,cassette
R2994 T4756 T4754 pobj cassette,of
R2995 T4757 T4756 compound PGKneo,cassette
R2996 T4758 T4749 cc and,interrupted
R2997 T4759 T4749 conj contained,interrupted
R2998 T4760 T4761 det a,cassette
R2999 T4761 T4759 dobj cassette,contained
R3000 T4762 T4763 compound MC1,TK
R3001 T4763 T4761 compound TK,cassette
R3002 T4764 T4763 punct /,TK
R3003 T4765 T4759 prep as,contained
R3004 T4766 T4767 det a,marker
R3005 T4767 T4765 pobj marker,as
R3006 T4768 T4767 amod negative,marker
R3007 T4769 T4767 compound selection,marker
R3008 T4770 T4767 prep against,marker
R3009 T4771 T4772 amod random,integration
R3010 T4772 T4770 pobj integration,against
R3011 T4773 T4774 punct [,5
R3012 T4774 T4739 parataxis 5,consisted
R3013 T4775 T4774 punct ],5
R3014 T4776 T4739 punct .,consisted
R3019 T4852 T4851 prep of,Generation
R3020 T4853 T4854 npadvmod Adam22,deficient
R3021 T4854 T4855 amod deficient,mice
R3022 T4855 T4852 pobj mice,of
R3023 T4857 T4858 det The,vector
R3024 T4858 T4861 nsubjpass vector,electroporated
R3025 T4859 T4858 amod linearised,vector
R3026 T4860 T4858 compound targeting,vector
R3027 T4862 T4861 auxpass was,electroporated
R3028 T4863 T4861 prep into,electroporated
R3029 T4864 T4865 nmod TT2,cells
R3030 T4865 T4863 pobj cells,into
R3031 T4866 T4867 amod embryonic,stem
R3032 T4867 T4865 nmod stem,cells
R3033 T4868 T4867 punct (,stem
R3034 T4869 T4867 appos ES,stem
R3035 T4870 T4865 punct ),cells
R3036 T4871 T4872 punct [,31
R3037 T4872 T4861 parataxis 31,electroporated
R3038 T4873 T4872 punct ],31
R3039 T4874 T4861 punct .,electroporated
R3040 T4876 T4877 amod Homologous,recombinants
R3041 T4877 T4878 nsubjpass recombinants,selected
R3042 T4879 T4878 auxpass were,selected
R3043 T4880 T4878 prep by,selected
R3044 T4881 T4880 pobj PCR,by
R3045 T4882 T4878 punct .,selected
R3046 T4884 T4885 det The,DNA
R3047 T4885 T4888 nsubjpass DNA,amplified
R3048 T4886 T4885 amod extracted,DNA
R3049 T4887 T4885 amod genomic,DNA
R3050 T4889 T4885 prep from,DNA
R3051 T4890 T4891 det each,clone
R3052 T4891 T4889 pobj clone,from
R3053 T4892 T4888 auxpass was,amplified
R3054 T4893 T4888 advcl using,amplified
R3055 T4894 T4895 det the,primer
R3056 T4895 T4893 dobj primer,using
R3057 T4896 T4895 amod forward,primer
R3058 T4897 T4895 appos AGN2,primer
R3059 T4898 T4895 punct : ,primer
R3060 T4899 T4900 nummod 5,GCCTGCTTGCCGAATATCATGGTGGAAAAT
R3061 T4900 T4895 appos GCCTGCTTGCCGAATATCATGGTGGAAAAT,primer
R3062 T4901 T4899 punct ',5
R3063 T4902 T4900 punct -,GCCTGCTTGCCGAATATCATGGTGGAAAAT
R3064 T4903 T4900 punct -,GCCTGCTTGCCGAATATCATGGTGGAAAAT
R3065 T4904 T4900 nummod 3,GCCTGCTTGCCGAATATCATGGTGGAAAAT
R3066 T4905 T4900 punct ',GCCTGCTTGCCGAATATCATGGTGGAAAAT
R3067 T4906 T4895 prep in,primer
R3068 T4907 T4908 det the,cassette
R3069 T4908 T4906 pobj cassette,in
R3070 T4909 T4908 compound PGKneo,cassette
R3071 T4910 T4895 punct ", ",primer
R3072 T4911 T4895 cc and,primer
R3073 T4912 T4913 det the,primer
R3074 T4913 T4895 conj primer,primer
R3075 T4914 T4913 amod reverse,primer
R3076 T4915 T4913 appos MFP065R,primer
R3077 T4916 T4913 punct : ,primer
R3078 T4917 T4918 nummod 5,ACTATTTCTGTGATGAGGGCACAGCATC
R3079 T4918 T4913 appos ACTATTTCTGTGATGAGGGCACAGCATC,primer
R3080 T4919 T4917 punct ',5
R3081 T4920 T4918 punct -,ACTATTTCTGTGATGAGGGCACAGCATC
R3082 T4921 T4918 punct -,ACTATTTCTGTGATGAGGGCACAGCATC
R3083 T4922 T4918 nummod 3,ACTATTTCTGTGATGAGGGCACAGCATC
R3084 T4923 T4918 punct ',ACTATTTCTGTGATGAGGGCACAGCATC
R3085 T4924 T4913 prep outside,primer
R3086 T4925 T4926 det the,vector
R3087 T4926 T4924 pobj vector,outside
R3088 T4927 T4926 compound targeting,vector
R3089 T4928 T4888 punct .,amplified
R3090 T4930 T4931 amod Homologous,DNA
R3091 T4931 T4933 nsubjpass DNA,amplified
R3092 T4932 T4931 amod recombined,DNA
R3093 T4934 T4933 auxpass was,amplified
R3094 T4935 T4933 advmod efficiently,amplified
R3095 T4936 T4933 prep by,amplified
R3096 T4937 T4938 nummod 35,cycles
R3097 T4938 T4936 pobj cycles,by
R3098 T4939 T4938 prep of,cycles
R3099 T4940 T4941 det the,steps
R3100 T4941 T4939 pobj steps,of
R3101 T4942 T4941 amod following,steps
R3102 T4943 T4941 compound amplification,steps
R3103 T4944 T4941 punct (,steps
R3104 T4945 T4946 nummod 94,°C
R3105 T4946 T4941 appos °C,steps
R3106 T4947 T4946 punct -,°C
R3107 T4948 T4949 nummod 30,s
R3108 T4949 T4946 npadvmod s,°C
R3109 T4950 T4946 cc and,°C
R3110 T4951 T4952 nummod 68,°C
R3111 T4952 T4946 conj °C,°C
R3112 T4953 T4952 punct -,°C
R3113 T4954 T4955 nummod 5,min
R3114 T4955 T4952 npadvmod min,°C
R3115 T4956 T4933 punct ),amplified
R3116 T4957 T4933 punct .,amplified
R3117 T4959 T4960 det The,efficiency
R3118 T4960 T4962 nsubj efficiency,was
R3119 T4961 T4960 compound targeting,efficiency
R3120 T4963 T4960 prep of,efficiency
R3121 T4964 T4965 det this,construct
R3122 T4965 T4963 pobj construct,of
R3123 T4966 T4967 advmod about,4
R3124 T4967 T4968 nummod 4,%
R3125 T4968 T4962 attr %,was
R3126 T4969 T4962 punct .,was
R3127 T4971 T4972 advmod Correctly,targeted
R3128 T4972 T4973 amod targeted,clones
R3129 T4973 T4975 nsubjpass clones,injected
R3130 T4974 T4973 compound ES,clones
R3131 T4976 T4975 auxpass were,injected
R3132 T4977 T4975 prep into,injected
R3133 T4978 T4979 amod fertilized,eggs
R3134 T4979 T4977 pobj eggs,into
R3135 T4980 T4979 compound ICR,eggs
R3136 T4981 T4979 compound mouse,eggs
R3137 T4982 T4975 prep at,injected
R3138 T4983 T4984 det the,stage
R3139 T4984 T4982 pobj stage,at
R3140 T4985 T4986 nummod eight,cell
R3141 T4986 T4984 compound cell,stage
R3142 T4987 T4986 punct -,cell
R3143 T4988 T4975 punct .,injected
R3144 T4990 T4991 det The,chimeras
R3145 T4991 T4994 nsubjpass chimeras,mated
R3146 T4992 T4991 amod resulting,chimeras
R3147 T4993 T4991 amod male,chimeras
R3148 T4995 T4994 auxpass were,mated
R3149 T4996 T4994 prep with,mated
R3150 T4997 T4998 nmod C57BL,females
R3151 T4998 T4996 pobj females,with
R3152 T4999 T4997 punct /,C57BL
R3153 T5000 T4997 nummod 6N,C57BL
R3154 T5001 T4994 punct ", ",mated
R3155 T5002 T4994 cc and,mated
R3156 T5003 T5004 amod heterozygous,mice
R3157 T5004 T5008 nsubjpass mice,interbred
R3158 T5005 T5004 amod male,mice
R3159 T5006 T5005 cc and,male
R3160 T5007 T5005 conj female,male
R3161 T5008 T4994 conj interbred,mated
R3162 T5009 T5008 auxpass were,interbred
R3163 T5010 T5011 aux to,generate
R3164 T5011 T5008 advcl generate,interbred
R3165 T5012 T5013 amod homozygous,mice
R3166 T5013 T5011 dobj mice,generate
R3167 T5014 T5008 punct .,interbred
R3168 T5016 T5017 det The,phenotypes
R3169 T5017 T5019 nsubjpass phenotypes,observed
R3170 T5018 T5017 amod ataxic,phenotypes
R3171 T5020 T5017 prep of,phenotypes
R3172 T5021 T5022 amod homozygous,mice
R3173 T5022 T5020 pobj mice,of
R3174 T5023 T5019 auxpass were,observed
R3175 T5024 T5019 prep in,observed
R3176 T5025 T5026 nummod two,lines
R3177 T5026 T5024 pobj lines,in
R3178 T5027 T5026 amod independent,lines
R3179 T5028 T5019 punct .,observed
R3180 T5055 T5056 compound Southern,blot
R3181 T5056 T5057 compound blot,analysis
R3182 T5059 T5060 nmod Mouse,DNA
R3183 T5060 T5062 nsubjpass DNA,extracted
R3184 T5061 T5060 amod genomic,DNA
R3185 T5063 T5060 acl used,DNA
R3186 T5064 T5063 prep for,used
R3187 T5065 T5066 compound Southern,blot
R3188 T5066 T5067 compound blot,analysis
R3189 T5067 T5064 pobj analysis,for
R3190 T5068 T5062 auxpass was,extracted
R3191 T5069 T5062 prep from,extracted
R3192 T5070 T5071 det the,liver
R3193 T5071 T5069 pobj liver,from
R3194 T5072 T5071 prep of,liver
R3195 T5073 T5074 det each,genotype
R3196 T5074 T5072 pobj genotype,of
R3197 T5075 T5062 punct .,extracted
R3198 T5077 T5078 npadvmod BamHI,digested
R3199 T5078 T5080 amod digested,DNA
R3200 T5079 T5078 punct -,digested
R3201 T5080 T5082 nsubjpass DNA,probed
R3202 T5081 T5080 amod genomic,DNA
R3203 T5083 T5082 auxpass was,probed
R3204 T5084 T5082 prep with,probed
R3205 T5085 T5086 det the,fragment
R3206 T5086 T5084 pobj fragment,with
R3207 T5087 T5086 punct [,fragment
R3208 T5088 T5089 advmod 32P,labelled
R3209 T5089 T5086 amod labelled,fragment
R3210 T5090 T5089 punct ],labelled
R3211 T5091 T5089 punct -,labelled
R3212 T5092 T5093 nummod 0.4,kb
R3213 T5093 T5086 nmod kb,fragment
R3214 T5094 T5093 punct -,kb
R3215 T5095 T5096 nmod SpeI,BamHI
R3216 T5096 T5086 nmod BamHI,fragment
R3217 T5097 T5096 punct -,BamHI
R3218 T5098 T5086 amod genomic,fragment
R3219 T5099 T5086 punct ", ",fragment
R3220 T5100 T5101 dep which,located
R3221 T5101 T5086 relcl located,fragment
R3222 T5102 T5101 auxpass is,located
R3223 T5103 T5101 prep between,located
R3224 T5104 T5105 nmod exons,8
R3225 T5105 T5103 pobj 8,between
R3226 T5106 T5105 cc and,8
R3227 T5107 T5105 conj 9,8
R3228 T5108 T5082 punct .,probed
R3229 T5223 T5224 compound Antibody,production
R3230 T5226 T5227 npadvmod His,tagged
R3231 T5227 T5229 amod tagged,protein
R3232 T5228 T5227 punct -,tagged
R3233 T5229 T5235 nsubjpass protein,produced
R3234 T5230 T5227 cc and,tagged
R3235 T5231 T5232 npadvmod MBP,fused
R3236 T5232 T5227 conj fused,tagged
R3237 T5233 T5232 punct -,fused
R3238 T5234 T5229 amod recombinant,protein
R3239 T5236 T5229 acl containing,protein
R3240 T5237 T5238 amod cytoplasmic,domain
R3241 T5238 T5236 dobj domain,containing
R3242 T5239 T5238 punct (,domain
R3243 T5240 T5238 appos cp,domain
R3244 T5241 T5238 punct ),domain
R3245 T5242 T5238 prep of,domain
R3246 T5243 T5244 amod human,isoform
R3247 T5244 T5242 pobj isoform,of
R3248 T5245 T5244 compound ADAM22,isoform
R3249 T5246 T5244 nummod 1,isoform
R3250 T5247 T5248 punct (,acids
R3251 T5248 T5244 parataxis acids,isoform
R3252 T5249 T5248 dep position,acids
R3253 T5250 T5249 punct : ,position
R3254 T5251 T5252 nummod 756,906
R3255 T5252 T5249 appos 906,position
R3256 T5253 T5252 punct –,906
R3257 T5254 T5248 punct ", ",acids
R3258 T5255 T5248 nummod 151,acids
R3259 T5256 T5248 compound amino,acids
R3260 T5257 T5248 prep in,acids
R3261 T5258 T5257 pobj length,in
R3262 T5259 T5248 punct ),acids
R3263 T5260 T5235 auxpass were,produced
R3264 T5261 T5235 prep in,produced
R3265 T5262 T5263 compound E.,coli
R3266 T5263 T5261 pobj coli,in
R3267 T5264 T5235 punct .,produced
R3268 T5266 T5267 npadvmod His,tagged
R3269 T5267 T5269 amod tagged,protein
R3270 T5268 T5267 punct -,tagged
R3271 T5269 T5273 nsubjpass protein,purified
R3272 T5270 T5271 compound ADAM22,cp
R3273 T5271 T5269 compound cp,protein
R3274 T5272 T5271 punct -,cp
R3275 T5274 T5273 auxpass was,purified
R3276 T5275 T5273 prep in,purified
R3277 T5276 T5277 amod denatured,condition
R3278 T5277 T5275 pobj condition,in
R3279 T5278 T5273 advcl using,purified
R3280 T5279 T5280 compound Ni,NTA
R3281 T5280 T5282 compound NTA,resin
R3282 T5281 T5280 punct -,NTA
R3283 T5282 T5278 dobj resin,using
R3284 T5283 T5284 punct (,GmbH
R3285 T5284 T5282 parataxis GmbH,resin
R3286 T5285 T5284 compound Qiagen,GmbH
R3287 T5286 T5284 punct ),GmbH
R3288 T5287 T5273 cc and,purified
R3289 T5288 T5273 conj dialysed,purified
R3290 T5289 T5288 prep in,dialysed
R3291 T5290 T5289 pobj PBS,in
R3292 T5291 T5273 punct .,purified
R3293 T5293 T5294 amod Precipitated,protein
R3294 T5294 T5295 nsubjpass protein,recovered
R3295 T5295 T5297 ccomp recovered,used
R3296 T5296 T5295 auxpass was,recovered
R3297 T5298 T5295 cc and,recovered
R3298 T5299 T5300 auxpass was,mixed
R3299 T5300 T5295 conj mixed,recovered
R3300 T5301 T5300 prep with,mixed
R3301 T5302 T5303 poss Freund,adjuvant
R3302 T5303 T5301 pobj adjuvant,with
R3303 T5304 T5302 case 's,Freund
R3304 T5305 T5303 amod complete,adjuvant
R3305 T5306 T5307 punct (,Invitrogen
R3306 T5307 T5303 parataxis Invitrogen,adjuvant
R3307 T5308 T5307 punct ),Invitrogen
R3308 T5309 T5297 punct ", ",used
R3309 T5310 T5297 advmod then,used
R3310 T5311 T5297 punct ", ",used
R3311 T5312 T5313 det the,mixture
R3312 T5313 T5297 nsubjpass mixture,used
R3313 T5314 T5297 auxpass was,used
R3314 T5315 T5297 prep for,used
R3315 T5316 T5315 pobj immunization,for
R3316 T5317 T5316 prep of,immunization
R3317 T5318 T5317 pobj rabbits,of
R3318 T5319 T5297 punct .,used
R3319 T5321 T5322 det The,antiserum
R3320 T5322 T5323 nsubjpass antiserum,incubated
R3321 T5324 T5322 acl raised,antiserum
R3322 T5325 T5324 prep against,raised
R3323 T5326 T5327 det the,protein
R3324 T5327 T5325 pobj protein,against
R3325 T5328 T5329 npadvmod His,tagged
R3326 T5329 T5327 amod tagged,protein
R3327 T5330 T5329 punct -,tagged
R3328 T5331 T5332 compound ADAM22,cp
R3329 T5332 T5327 compound cp,protein
R3330 T5333 T5332 punct -,cp
R3331 T5334 T5323 auxpass was,incubated
R3332 T5335 T5323 prep with,incubated
R3333 T5336 T5337 npadvmod MBP,fused
R3334 T5337 T5339 amod fused,protein
R3335 T5338 T5337 punct -,fused
R3336 T5339 T5335 pobj protein,with
R3337 T5340 T5341 compound ADAM22,cp
R3338 T5341 T5339 compound cp,protein
R3339 T5342 T5341 punct -,cp
R3340 T5343 T5339 acl coupled,protein
R3341 T5344 T5343 prep to,coupled
R3342 T5345 T5346 nmod Affi,Gel
R3343 T5346 T5348 nmod Gel,beads
R3344 T5347 T5346 punct -,Gel
R3345 T5348 T5344 pobj beads,to
R3346 T5349 T5346 nummod 10,Gel
R3347 T5350 T5351 punct (,Rad
R3348 T5351 T5348 parataxis Rad,beads
R3349 T5352 T5351 compound Bio,Rad
R3350 T5353 T5351 punct -,Rad
R3351 T5354 T5351 punct ),Rad
R3352 T5355 T5323 punct .,incubated
R3353 T5357 T5358 nsubjpass Beads,washed
R3354 T5359 T5357 prep with,Beads
R3355 T5360 T5361 amod bound,antibodies
R3356 T5361 T5359 pobj antibodies,with
R3357 T5362 T5358 auxpass were,washed
R3358 T5363 T5358 prep in,washed
R3359 T5364 T5363 pobj PBS,in
R3360 T5365 T5358 punct ", ",washed
R3361 T5366 T5358 cc and,washed
R3362 T5367 T5368 det the,antibodies
R3363 T5368 T5370 nsubjpass antibodies,eluted
R3364 T5369 T5368 amod bound,antibodies
R3365 T5370 T5358 conj eluted,washed
R3366 T5371 T5370 auxpass were,eluted
R3367 T5372 T5370 prep with,eluted
R3368 T5373 T5374 nummod 100,mM
R3369 T5374 T5375 compound mM,glycine
R3370 T5375 T5372 pobj glycine,with
R3371 T5376 T5375 punct ", ",glycine
R3372 T5377 T5375 npadvmod pH,glycine
R3373 T5378 T5377 nummod 3.0,pH
R3374 T5379 T5370 punct ", ",eluted
R3375 T5380 T5370 cc and,eluted
R3376 T5381 T5370 conj neutralized,eluted
R3377 T5382 T5381 advmod immediately,neutralized
R3378 T5383 T5370 punct .,eluted
R3379 T5385 T5386 advcl Using,detected
R3380 T5387 T5388 det the,antibody
R3381 T5388 T5385 dobj antibody,Using
R3382 T5389 T5390 npadvmod affinity,purified
R3383 T5390 T5388 amod purified,antibody
R3384 T5391 T5386 punct ", ",detected
R3385 T5392 T5393 preconj both,mouse
R3386 T5393 T5396 compound mouse,proteins
R3387 T5394 T5393 amod human,mouse
R3388 T5395 T5393 cc and,mouse
R3389 T5396 T5386 nsubjpass proteins,detected
R3390 T5397 T5396 compound ADAM22,proteins
R3391 T5398 T5386 auxpass were,detected
R3392 T5399 T5386 prep by,detected
R3393 T5400 T5401 compound Western,blot
R3394 T5401 T5402 compound blot,analysis
R3395 T5402 T5399 pobj analysis,by
R3396 T5403 T5386 punct .,detected
R3397 T5405 T5406 advmod However,was
R3398 T5407 T5406 punct ", ",was
R3399 T5408 T5406 advmod unfortunately,was
R3400 T5409 T5410 det the,antibody
R3401 T5410 T5406 nsubj antibody,was
R3402 T5411 T5406 neg not,was
R3403 T5412 T5406 acomp suitable,was
R3404 T5413 T5412 prep for,suitable
R3405 T5414 T5415 amod immunohistochemical,analysis
R3406 T5415 T5413 pobj analysis,for
R3407 T5416 T5415 prep of,analysis
R3408 T5417 T5418 compound mouse,tissues
R3409 T5418 T5416 pobj tissues,of
R3410 T5419 T5406 punct .,was
R3411 T5499 T5500 compound Western,blot
R3412 T5500 T5501 compound blot,analysis
R3413 T5503 T5504 det The,absence
R3414 T5504 T5505 nsubjpass absence,confirmed
R3415 T5506 T5504 prep of,absence
R3416 T5507 T5508 compound ADAM22,protein
R3417 T5508 T5506 pobj protein,of
R3418 T5509 T5504 prep in,absence
R3419 T5510 T5511 det the,mice
R3420 T5511 T5509 pobj mice,in
R3421 T5512 T5511 nmod Adam22,mice
R3422 T5513 T5512 punct -,Adam22
R3423 T5514 T5512 punct /,Adam22
R3424 T5515 T5512 punct -,Adam22
R3425 T5516 T5505 auxpass was,confirmed
R3426 T5517 T5505 prep by,confirmed
R3427 T5518 T5519 compound Western,blot
R3428 T5519 T5520 compound blot,analysis
R3429 T5520 T5517 pobj analysis,by
R3430 T5521 T5505 punct .,confirmed
R3431 T5523 T5524 advmod Briefly,isolated
R3432 T5525 T5524 punct ", ",isolated
R3433 T5526 T5527 det the,cerebellum
R3434 T5527 T5524 nsubjpass cerebellum,isolated
R3435 T5528 T5524 auxpass was,isolated
R3436 T5529 T5524 prep from,isolated
R3437 T5530 T5531 det a,mouse
R3438 T5531 T5529 pobj mouse,from
R3439 T5532 T5531 compound P14.5,mouse
R3440 T5533 T5531 prep of,mouse
R3441 T5534 T5535 det each,genotype
R3442 T5535 T5533 pobj genotype,of
R3443 T5536 T5524 cc and,isolated
R3444 T5537 T5524 conj homogenized,isolated
R3445 T5538 T5537 prep with,homogenized
R3446 T5539 T5540 det a,homogenizer
R3447 T5540 T5538 pobj homogenizer,with
R3448 T5541 T5540 compound Polytron,homogenizer
R3449 T5542 T5540 prep in,homogenizer
R3450 T5543 T5544 compound cell,lysis
R3451 T5544 T5545 compound lysis,buffer
R3452 T5545 T5542 pobj buffer,in
R3453 T5546 T5547 punct (,Diagnostics
R3454 T5547 T5545 parataxis Diagnostics,buffer
R3455 T5548 T5549 nummod 50,mM
R3456 T5549 T5550 compound mM,HCl
R3457 T5550 T5547 dep HCl,Diagnostics
R3458 T5551 T5550 compound Tris,HCl
R3459 T5552 T5550 punct -,HCl
R3460 T5553 T5550 punct ", ",HCl
R3461 T5554 T5550 npadvmod pH,HCl
R3462 T5555 T5554 nummod 7.5,pH
R3463 T5556 T5550 punct ", ",HCl
R3464 T5557 T5558 nummod 100,mM
R3465 T5558 T5559 compound mM,NaCl
R3466 T5559 T5550 appos NaCl,HCl
R3467 T5560 T5550 punct ", ",HCl
R3468 T5561 T5562 nummod 1,%
R3469 T5562 T5563 compound %,NP
R3470 T5563 T5550 appos NP,HCl
R3471 T5564 T5563 punct -,NP
R3472 T5565 T5563 nummod 40,NP
R3473 T5566 T5550 punct ", ",HCl
R3474 T5567 T5568 amod Complete,inhibitors
R3475 T5568 T5550 appos inhibitors,HCl
R3476 T5569 T5568 compound protease,inhibitors
R3477 T5570 T5547 punct [,Diagnostics
R3478 T5571 T5547 compound Roche,Diagnostics
R3479 T5572 T5547 punct ],Diagnostics
R3480 T5573 T5547 punct ),Diagnostics
R3481 T5574 T5524 punct .,isolated
R3482 T5576 T5577 prep After,separated
R3483 T5578 T5576 pobj removal,After
R3484 T5579 T5578 prep of,removal
R3485 T5580 T5581 compound cell,debris
R3486 T5581 T5579 pobj debris,of
R3487 T5582 T5578 prep by,removal
R3488 T5583 T5582 pobj centrifugation,by
R3489 T5584 T5577 punct ", ",separated
R3490 T5585 T5586 det the,supernatant
R3491 T5586 T5577 nsubjpass supernatant,separated
R3492 T5587 T5577 auxpass was,separated
R3493 T5588 T5577 prep on,separated
R3494 T5589 T5590 nummod 10,%
R3495 T5590 T5591 compound %,PAGE
R3496 T5591 T5588 pobj PAGE,on
R3497 T5592 T5591 compound SDS,PAGE
R3498 T5593 T5591 punct -,PAGE
R3499 T5594 T5577 punct ", ",separated
R3500 T5595 T5577 cc and,separated
R3501 T5596 T5577 conj transferred,separated
R3502 T5597 T5596 prep to,transferred
R3503 T5598 T5599 det a,membrane
R3504 T5599 T5597 pobj membrane,to
R3505 T5600 T5599 compound nitrocellulose,membrane
R3506 T5601 T5577 punct .,separated
R3507 T5603 T5604 det The,blot
R3508 T5604 T5605 nsubjpass blot,incubated
R3509 T5606 T5605 auxpass was,incubated
R3510 T5607 T5605 advmod then,incubated
R3511 T5608 T5605 prep with,incubated
R3512 T5609 T5610 amod polyclonal,antibody
R3513 T5610 T5608 pobj antibody,with
R3514 T5611 T5610 prep against,antibody
R3515 T5612 T5613 det the,domain
R3516 T5613 T5611 pobj domain,against
R3517 T5614 T5613 amod cytoplasmic,domain
R3518 T5615 T5613 prep of,domain
R3519 T5616 T5615 pobj ADAM22,of
R3520 T5617 T5610 punct (,antibody
R3521 T5618 T5610 prep at,antibody
R3522 T5619 T5618 pobj 1,at
R3523 T5620 T5621 punct :,"1,000"
R3524 T5621 T5619 prep "1,000",1
R3525 T5622 T5605 punct ),incubated
R3526 T5623 T5605 punct .,incubated
R3527 T5625 T5626 amod Bound,antibodies
R3528 T5626 T5627 nsubjpass antibodies,visualized
R3529 T5628 T5627 auxpass were,visualized
R3530 T5629 T5627 prep with,visualized
R3531 T5630 T5631 nmod horseradish,antibody
R3532 T5631 T5629 pobj antibody,with
R3533 T5632 T5633 npadvmod peroxidase,labelled
R3534 T5633 T5631 amod labelled,antibody
R3535 T5634 T5633 punct -,labelled
R3536 T5635 T5631 amod second,antibody
R3537 T5636 T5631 cc and,antibody
R3538 T5637 T5638 det a,system
R3539 T5638 T5631 conj system,antibody
R3540 T5639 T5640 compound ECL,plus
R3541 T5640 T5638 compound plus,system
R3542 T5641 T5640 punct -,plus
R3543 T5642 T5638 compound chemiluminescence,system
R3544 T5643 T5638 compound detection,system
R3545 T5644 T5645 punct (,Corp.
R3546 T5645 T5638 parataxis Corp.,system
R3547 T5646 T5645 compound Amersham,Corp.
R3548 T5647 T5645 compound Biosciences,Corp.
R3549 T5648 T5645 punct ),Corp.
R3550 T5649 T5627 punct .,visualized
R3557 T5703 T5704 amod Primary,cell
R3558 T5704 T5706 compound cell,culture
R3559 T5705 T5704 compound Schwann,cell
R3560 T5708 T5709 amod Primary,cells
R3561 T5709 T5711 nsubjpass cells,prepared
R3562 T5710 T5709 compound Schwann,cells
R3563 T5712 T5711 auxpass were,prepared
R3564 T5713 T5711 prep from,prepared
R3565 T5714 T5715 nummod 4,month
R3566 T5715 T5717 npadvmod month,old
R3567 T5716 T5715 punct -,month
R3568 T5717 T5719 amod old,mice
R3569 T5718 T5717 punct -,old
R3570 T5719 T5713 pobj mice,from
R3571 T5720 T5719 nmod C57BL,mice
R3572 T5721 T5720 punct /,C57BL
R3573 T5722 T5720 nummod 6,C57BL
R3574 T5723 T5711 prep according,prepared
R3575 T5724 T5723 prep to,according
R3576 T5725 T5726 poss Manent,protocol
R3577 T5726 T5724 pobj protocol,to
R3578 T5727 T5725 case 's,Manent
R3579 T5728 T5729 punct [,32
R3580 T5729 T5726 parataxis 32,protocol
R3581 T5730 T5729 punct ],32
R3582 T5731 T5726 prep with,protocol
R3583 T5732 T5733 amod minor,modifications
R3584 T5733 T5731 pobj modifications,with
R3585 T5734 T5711 punct .,prepared
R3586 T5736 T5737 advmod Briefly,removed
R3587 T5738 T5737 punct ", ",removed
R3588 T5739 T5740 amod sciatic,nerves
R3589 T5740 T5737 nsubjpass nerves,removed
R3590 T5741 T5737 auxpass were,removed
R3591 T5742 T5737 cc and,removed
R3592 T5743 T5737 conj incubated,removed
R3593 T5744 T5743 prep for,incubated
R3594 T5745 T5746 nummod 7,days
R3595 T5746 T5744 pobj days,for
R3596 T5747 T5743 prep in,incubated
R3597 T5748 T5749 det the,medium
R3598 T5749 T5747 pobj medium,in
R3599 T5750 T5749 amod pre-treatment,medium
R3600 T5751 T5749 punct ", ",medium
R3601 T5752 T5753 dep which,consisted
R3602 T5753 T5749 relcl consisted,medium
R3603 T5754 T5753 prep of,consisted
R3604 T5755 T5756 compound D,MEM
R3605 T5756 T5754 pobj MEM,of
R3606 T5757 T5756 punct -,MEM
R3607 T5758 T5759 punct (,glucose
R3608 T5759 T5756 parataxis glucose,MEM
R3609 T5760 T5759 amod high,glucose
R3610 T5761 T5759 punct ),glucose
R3611 T5762 T5756 acl supplemented,MEM
R3612 T5763 T5762 prep with,supplemented
R3613 T5764 T5765 nummod 10,%
R3614 T5765 T5766 compound %,FCS
R3615 T5766 T5763 pobj FCS,with
R3616 T5767 T5766 punct ", ",FCS
R3617 T5768 T5769 nummod 50,mg
R3618 T5769 T5770 nmod mg,gentamicin
R3619 T5770 T5766 conj gentamicin,FCS
R3620 T5771 T5772 punct /,ml
R3621 T5772 T5769 prep ml,mg
R3622 T5773 T5774 punct (,Corp.
R3623 T5774 T5770 parataxis Corp.,gentamicin
R3624 T5775 T5774 compound Invitrogen,Corp.
R3625 T5776 T5774 punct ),Corp.
R3626 T5777 T5770 punct ", ",gentamicin
R3627 T5778 T5779 nummod 2.5,μg
R3628 T5779 T5780 nmod μg,fungizone
R3629 T5780 T5770 conj fungizone,gentamicin
R3630 T5781 T5782 punct /,ml
R3631 T5782 T5779 prep ml,μg
R3632 T5783 T5784 punct (,Corp.
R3633 T5784 T5780 parataxis Corp.,fungizone
R3634 T5785 T5784 compound Invitrogen,Corp.
R3635 T5786 T5784 punct ),Corp.
R3636 T5787 T5780 punct ", ",fungizone
R3637 T5788 T5789 nummod 2,μM
R3638 T5789 T5790 compound μM,forskolin
R3639 T5790 T5780 conj forskolin,fungizone
R3640 T5791 T5792 punct (,Inc.
R3641 T5792 T5790 parataxis Inc.,forskolin
R3642 T5793 T5792 compound EMD,Inc.
R3643 T5794 T5792 compound Biosciences,Inc.
R3644 T5795 T5792 punct ),Inc.
R3645 T5796 T5790 cc and,forskolin
R3646 T5797 T5798 nummod 10,ng
R3647 T5798 T5799 nmod ng,beta1
R3648 T5799 T5790 conj beta1,forskolin
R3649 T5800 T5801 punct /,ml
R3650 T5801 T5798 prep ml,ng
R3651 T5802 T5799 amod recombinant,beta1
R3652 T5803 T5799 compound heregulin,beta1
R3653 T5804 T5799 punct -,beta1
R3654 T5805 T5806 punct (,Systems
R3655 T5806 T5799 parataxis Systems,beta1
R3656 T5807 T5806 nmod R,Systems
R3657 T5808 T5807 cc &,R
R3658 T5809 T5807 conj D,R
R3659 T5810 T5806 punct ),Systems
R3660 T5811 T5737 punct .,removed
R3661 T5813 T5814 prep For,cut
R3662 T5815 T5813 pobj dissociation,For
R3663 T5816 T5814 punct ", ",cut
R3664 T5817 T5818 amod cultured,nerves
R3665 T5818 T5814 nsubjpass nerves,cut
R3666 T5819 T5818 amod sciatic,nerves
R3667 T5820 T5814 auxpass were,cut
R3668 T5821 T5814 prep into,cut
R3669 T5822 T5821 pobj pieces,into
R3670 T5823 T5814 cc and,cut
R3671 T5824 T5814 conj incubated,cut
R3672 T5825 T5824 prep at,incubated
R3673 T5826 T5827 nummod 37,°C
R3674 T5827 T5825 pobj °C,at
R3675 T5828 T5824 prep for,incubated
R3676 T5829 T5830 nummod 3,hours
R3677 T5830 T5828 pobj hours,for
R3678 T5831 T5824 prep in,incubated
R3679 T5832 T5833 compound Opti,MEM
R3680 T5833 T5835 compound MEM,medium
R3681 T5834 T5833 punct -,MEM
R3682 T5835 T5831 pobj medium,in
R3683 T5836 T5837 punct (,Corp.
R3684 T5837 T5835 parataxis Corp.,medium
R3685 T5838 T5837 compound Invitrogen,Corp.
R3686 T5839 T5837 punct ),Corp.
R3687 T5840 T5835 acl containing,medium
R3688 T5841 T5842 nummod 130,U
R3689 T5842 T5843 nmod U,type
R3690 T5843 T5840 dobj type,containing
R3691 T5844 T5845 punct /,ml
R3692 T5845 T5842 prep ml,U
R3693 T5846 T5843 compound collagenase,type
R3694 T5847 T5843 nummod I,type
R3695 T5848 T5849 punct (,Corp.
R3696 T5849 T5843 parataxis Corp.,type
R3697 T5850 T5849 compound Invitrogen,Corp.
R3698 T5851 T5849 punct ),Corp.
R3699 T5852 T5843 cc and,type
R3700 T5853 T5854 nummod 0.4,U
R3701 T5854 T5855 nmod U,dispase
R3702 T5855 T5843 conj dispase,type
R3703 T5856 T5857 punct /,ml
R3704 T5857 T5854 prep ml,U
R3705 T5858 T5855 nummod II,dispase
R3706 T5859 T5860 punct (,Diagnostics
R3707 T5860 T5855 parataxis Diagnostics,dispase
R3708 T5861 T5860 compound Roche,Diagnostics
R3709 T5862 T5860 punct ),Diagnostics
R3710 T5863 T5814 punct .,cut
R3711 T5865 T5866 amod Dissociated,cells
R3712 T5866 T5867 nsubjpass cells,resuspended
R3713 T5868 T5867 auxpass were,resuspended
R3714 T5869 T5867 prep in,resuspended
R3715 T5870 T5871 det the,medium
R3716 T5871 T5869 pobj medium,in
R3717 T5872 T5871 amod pre-treatment,medium
R3718 T5873 T5867 cc and,resuspended
R3719 T5874 T5867 conj plated,resuspended
R3720 T5875 T5874 prep on,plated
R3721 T5876 T5877 compound Poly,Lysine
R3722 T5877 T5881 npadvmod Lysine,coated
R3723 T5878 T5877 punct -,Lysine
R3724 T5879 T5877 compound D,Lysine
R3725 T5880 T5877 punct -,Lysine
R3726 T5881 T5884 amod coated,plate
R3727 T5882 T5877 punct /,Lysine
R3728 T5883 T5877 appos Laminin,Lysine
R3729 T5884 T5875 pobj plate,on
R3730 T5885 T5886 punct (,Biosciences
R3731 T5886 T5884 parataxis Biosciences,plate
R3732 T5887 T5886 compound BD,Biosciences
R3733 T5888 T5886 punct ),Biosciences
R3734 T5889 T5867 punct .,resuspended
R3735 T5891 T5892 det The,purity
R3736 T5892 T5893 nsubj purity,approached
R3737 T5894 T5892 prep of,purity
R3738 T5895 T5896 det the,cells
R3739 T5896 T5894 pobj cells,of
R3740 T5897 T5896 amod cultured,cells
R3741 T5898 T5896 compound Schwann,cells
R3742 T5899 T5892 punct ", ",purity
R3743 T5900 T5901 mark as,determined
R3744 T5901 T5892 advcl determined,purity
R3745 T5902 T5901 prep by,determined
R3746 T5903 T5904 amod indirect,analysis
R3747 T5904 T5902 pobj analysis,by
R3748 T5905 T5904 compound immunofluorescence,analysis
R3749 T5906 T5893 punct ", ",approached
R3750 T5907 T5908 nummod 90,%
R3751 T5908 T5893 dobj %,approached
R3752 T5909 T5893 punct .,approached
R3755 T5980 T5981 compound RT,PCR
R3756 T5981 T5983 compound PCR,analysis
R3757 T5982 T5981 punct -,PCR
R3758 T5985 T5986 amod Adult,mice
R3759 T5986 T5991 nsubjpass mice,used
R3760 T5987 T5986 nmod C57BL,mice
R3761 T5988 T5987 punct /,C57BL
R3762 T5989 T5987 nummod 6,C57BL
R3763 T5990 T5986 amod male,mice
R3764 T5992 T5991 auxpass were,used
R3765 T5993 T5991 prep in,used
R3766 T5994 T5995 det this,study
R3767 T5995 T5993 pobj study,in
R3768 T5996 T5991 punct .,used
R3769 T5998 T5999 amod Total,RNAs
R3770 T5999 T6000 nsubjpass RNAs,analysed
R3771 T6001 T5999 acl purified,RNAs
R3772 T6002 T6001 prep from,purified
R3773 T6003 T6004 det the,cerebellum
R3774 T6004 T6002 pobj cerebellum,from
R3775 T6005 T6004 punct ", ",cerebellum
R3776 T6006 T6007 amod spinal,cord
R3777 T6007 T6004 conj cord,cerebellum
R3778 T6008 T6007 punct ", ",cord
R3779 T6009 T6010 amod sciatic,nerve
R3780 T6010 T6007 conj nerve,cord
R3781 T6011 T6010 punct ", ",nerve
R3782 T6012 T6010 conj DRG,nerve
R3783 T6013 T6012 cc and,DRG
R3784 T6014 T6015 amod cultured,cells
R3785 T6015 T6012 conj cells,DRG
R3786 T6016 T6015 compound Schwann,cells
R3787 T6017 T6001 advcl using,purified
R3788 T6018 T6017 dobj TRIzol,using
R3789 T6019 T6020 punct (,Corp.
R3790 T6020 T6018 parataxis Corp.,TRIzol
R3791 T6021 T6020 compound Invitrogen,Corp.
R3792 T6022 T6020 punct ),Corp.
R3793 T6023 T6018 cc and,TRIzol
R3794 T6024 T6025 compound RNeasy,kit
R3795 T6025 T6018 conj kit,TRIzol
R3796 T6026 T6027 punct (,GmbH
R3797 T6027 T6025 parataxis GmbH,kit
R3798 T6028 T6027 compound Qiagen,GmbH
R3799 T6029 T6027 punct ),GmbH
R3800 T6030 T6000 auxpass were,analysed
R3801 T6031 T6000 prep by,analysed
R3802 T6032 T6033 compound RT,PCR
R3803 T6033 T6031 pobj PCR,by
R3804 T6034 T6033 punct -,PCR
R3805 T6035 T6000 advcl using,analysed
R3806 T6036 T6035 dobj SuperScript,using
R3807 T6037 T6036 nummod II,SuperScript
R3808 T6038 T6036 cc and,SuperScript
R3809 T6039 T6040 amod random,primer
R3810 T6040 T6036 conj primer,SuperScript
R3811 T6041 T6042 punct (,Corp.
R3812 T6042 T6040 parataxis Corp.,primer
R3813 T6043 T6042 compound Invitrogen,Corp.
R3814 T6044 T6042 punct ),Corp.
R3815 T6045 T6036 punct ", ",SuperScript
R3816 T6046 T6036 cc and,SuperScript
R3817 T6047 T6048 compound PCR,amplification
R3818 T6048 T6036 conj amplification,SuperScript
R3819 T6049 T6050 punct (,°C
R3820 T6050 T6048 parataxis °C,amplification
R3821 T6051 T6052 nummod 40,cycles
R3822 T6052 T6050 dep cycles,°C
R3823 T6053 T6050 punct ;,°C
R3824 T6054 T6050 nummod 94,°C
R3825 T6055 T6050 punct -,°C
R3826 T6056 T6057 nummod 30,s
R3827 T6057 T6050 npadvmod s,°C
R3828 T6058 T6050 punct ", ",°C
R3829 T6059 T6060 nummod 60,°C
R3830 T6060 T6050 conj °C,°C
R3831 T6061 T6060 punct -,°C
R3832 T6062 T6063 nummod 30,s
R3833 T6063 T6060 npadvmod s,°C
R3834 T6064 T6060 cc and,°C
R3835 T6065 T6066 nummod 68,°C
R3836 T6066 T6060 conj °C,°C
R3837 T6067 T6066 punct -,°C
R3838 T6068 T6069 nummod 5,min
R3839 T6069 T6066 npadvmod min,°C
R3840 T6070 T6050 punct ),°C
R3841 T6071 T6048 prep with,amplification
R3842 T6072 T6073 compound Expand,polymerase
R3843 T6073 T6071 pobj polymerase,with
R3844 T6074 T6075 compound Hi,Fidelity
R3845 T6075 T6073 compound Fidelity,polymerase
R3846 T6076 T6075 punct -,Fidelity
R3847 T6077 T6073 compound DNA,polymerase
R3848 T6078 T6079 punct (,Diagnostics
R3849 T6079 T6073 parataxis Diagnostics,polymerase
R3850 T6080 T6079 compound Roche,Diagnostics
R3851 T6081 T6079 punct ),Diagnostics
R3852 T6082 T6073 cc and,polymerase
R3853 T6083 T6084 npadvmod ADAM22,specific
R3854 T6084 T6086 amod specific,primers
R3855 T6085 T6084 punct -,specific
R3856 T6086 T6073 conj primers,polymerase
R3857 T6087 T6000 punct .,analysed
R3858 T6089 T6090 aux To,detect
R3859 T6090 T6091 advcl detect,placed
R3860 T6092 T6093 compound splicing,variants
R3861 T6093 T6090 dobj variants,detect
R3862 T6094 T6090 prep in,detect
R3863 T6095 T6096 det the,domain
R3864 T6096 T6094 pobj domain,in
R3865 T6097 T6096 amod cytoplasmic,domain
R3866 T6098 T6091 punct ", ",placed
R3867 T6099 T6100 det a,primer
R3868 T6100 T6091 nsubjpass primer,placed
R3869 T6101 T6100 amod forward,primer
R3870 T6102 T6091 auxpass was,placed
R3871 T6103 T6104 advmod just,upstream
R3872 T6104 T6091 advmod upstream,placed
R3873 T6105 T6104 prep of,upstream
R3874 T6106 T6107 det the,domain
R3875 T6107 T6105 pobj domain,of
R3876 T6108 T6107 amod transmembrane,domain
R3877 T6109 T6091 cc and,placed
R3878 T6110 T6111 det a,primer
R3879 T6111 T6113 nsubjpass primer,set
R3880 T6112 T6111 amod reverse,primer
R3881 T6113 T6091 conj set,placed
R3882 T6114 T6113 auxpass was,set
R3883 T6115 T6113 prep on,set
R3884 T6116 T6117 det the,exon
R3885 T6117 T6115 pobj exon,on
R3886 T6118 T6117 amod terminating,exon
R3887 T6119 T6091 punct .,placed
R3888 T6121 T6122 det The,primers
R3889 T6122 T6123 nsubj primers,were
R3890 T6124 T6122 acl used,primers
R3891 T6125 T6124 prep in,used
R3892 T6126 T6127 det this,study
R3893 T6127 T6125 pobj study,in
R3894 T6128 T6129 mark as,follows
R3895 T6129 T6123 advcl follows,were
R3896 T6130 T6123 punct .,were
R3897 T6133 T6132 punct -,ISH04
R3898 T6134 T6132 amod forward,ISH04
R3899 T6135 T6132 punct : ,ISH04
R3900 T6136 T6137 nummod 5,AACAGGCACTGGACAGGGGCTGAC
R3901 T6137 T6132 appos AACAGGCACTGGACAGGGGCTGAC,ISH04
R3902 T6138 T6136 punct ',5
R3903 T6139 T6137 punct -,AACAGGCACTGGACAGGGGCTGAC
R3904 T6140 T6137 punct -,AACAGGCACTGGACAGGGGCTGAC
R3905 T6141 T6137 nummod 3,AACAGGCACTGGACAGGGGCTGAC
R3906 T6142 T6137 punct ',AACAGGCACTGGACAGGGGCTGAC
R3907 T6143 T6132 cc and,ISH04
R3908 T6144 T6132 conj ISH04,ISH04
R3909 T6145 T6144 punct -,ISH04
R3910 T6146 T6144 amod reverse,ISH04
R3911 T6147 T6144 punct : ,ISH04
R3912 T6148 T6149 nummod 5,AATGGATGTCTCCCATAGCCTGGC
R3913 T6149 T6144 appos AATGGATGTCTCCCATAGCCTGGC,ISH04
R3914 T6150 T6148 punct ',5
R3915 T6151 T6149 punct -,AATGGATGTCTCCCATAGCCTGGC
R3916 T6152 T6149 punct -,AATGGATGTCTCCCATAGCCTGGC
R3917 T6153 T6149 nummod 3,AATGGATGTCTCCCATAGCCTGGC
R3918 T6154 T6149 punct ',AATGGATGTCTCCCATAGCCTGGC
R3919 T6155 T6144 punct .,ISH04
R3920 T6214 T6215 nsubjpass Mice,anesthetized
R3921 T6216 T6215 auxpass were,anesthetized
R3922 T6217 T6215 prep with,anesthetized
R3923 T6218 T6219 compound ethyl,ether
R3924 T6219 T6217 pobj ether,with
R3925 T6220 T6215 punct .,anesthetized
R3926 T6222 T6223 amod Whole,body
R3927 T6223 T6225 compound body,perfusion
R3928 T6224 T6223 punct -,body
R3929 T6225 T6226 nsubjpass perfusion,performed
R3930 T6227 T6225 prep by,perfusion
R3931 T6228 T6229 nummod 2,%
R3932 T6229 T6230 compound %,solution
R3933 T6230 T6227 pobj solution,by
R3934 T6231 T6232 compound paraformaldehyde,glutaraldehyde
R3935 T6232 T6230 compound glutaraldehyde,solution
R3936 T6233 T6232 punct -,glutaraldehyde
R3937 T6234 T6225 acl followed,perfusion
R3938 T6235 T6234 agent by,followed
R3939 T6236 T6237 npadvmod heparin,included
R3940 T6237 T6239 amod included,saline
R3941 T6238 T6237 punct -,included
R3942 T6239 T6235 pobj saline,by
R3943 T6240 T6226 auxpass was,performed
R3944 T6241 T6226 punct .,performed
R3945 T6243 T6244 amod Sciatic,nerves
R3946 T6244 T6245 nsubjpass nerves,removed
R3947 T6246 T6244 punct ", ",nerves
R3948 T6247 T6248 amod trigeminal,nerves
R3949 T6248 T6244 conj nerves,nerves
R3950 T6249 T6248 punct ", ",nerves
R3951 T6250 T6248 conj brain,nerves
R3952 T6251 T6250 cc and,brain
R3953 T6252 T6253 amod spinal,cord
R3954 T6253 T6250 conj cord,brain
R3955 T6254 T6245 auxpass were,removed
R3956 T6255 T6245 cc and,removed
R3957 T6256 T6245 conj fixed,removed
R3958 T6257 T6256 prep in,fixed
R3959 T6258 T6259 nummod 10,%
R3960 T6259 T6260 nmod %,formalin
R3961 T6260 T6257 pobj formalin,in
R3962 T6261 T6262 amod neutral,buffered
R3963 T6262 T6260 amod buffered,formalin
R3964 T6263 T6262 punct -,buffered
R3965 T6264 T6245 punct .,removed
R3966 T6266 T6267 det The,cord
R3967 T6267 T6269 nsubjpass cord,washed
R3968 T6268 T6267 amod spinal,cord
R3969 T6270 T6267 cc and,cord
R3970 T6271 T6272 amod other,blocks
R3971 T6272 T6267 conj blocks,cord
R3972 T6273 T6272 compound nerve,blocks
R3973 T6274 T6269 auxpass were,washed
R3974 T6275 T6269 cc and,washed
R3975 T6276 T6269 conj postfixed,washed
R3976 T6277 T6276 prep with,postfixed
R3977 T6278 T6279 nummod 2,%
R3978 T6279 T6280 compound %,tetroxide
R3979 T6280 T6277 pobj tetroxide,with
R3980 T6281 T6280 compound osmium,tetroxide
R3981 T6282 T6269 punct ", ",washed
R3982 T6283 T6269 cc and,washed
R3983 T6284 T6285 auxpass were,dehydrated
R3984 T6285 T6269 conj dehydrated,washed
R3985 T6286 T6285 prep in,dehydrated
R3986 T6287 T6286 pobj ethanol,in
R3987 T6288 T6285 cc and,dehydrated
R3988 T6289 T6285 conj equilibrated,dehydrated
R3989 T6290 T6289 prep in,equilibrated
R3990 T6291 T6290 pobj Epon,in
R3991 T6292 T6269 punct .,washed
R3992 T6294 T6295 npadvmod Epon,embedded
R3993 T6295 T6296 amod embedded,sections
R3994 T6296 T6298 nsubjpass sections,stained
R3995 T6297 T6296 amod semithin,sections
R3996 T6299 T6298 auxpass were,stained
R3997 T6300 T6298 prep with,stained
R3998 T6301 T6302 compound toluidine,blue
R3999 T6302 T6300 pobj blue,with
R4000 T6303 T6298 cc and,stained
R4001 T6304 T6305 auxpass were,subjected
R4002 T6305 T6298 conj subjected,stained
R4003 T6306 T6305 prep to,subjected
R4004 T6307 T6308 npadvmod light,microscopic
R4005 T6308 T6309 amod microscopic,examination
R4006 T6309 T6306 pobj examination,to
R4007 T6310 T6298 punct .,stained
R4008 T6312 T6313 prep For,cut
R4009 T6314 T6315 npadvmod electron,microscopic
R4010 T6315 T6316 amod microscopic,analysis
R4011 T6316 T6312 pobj analysis,For
R4012 T6317 T6313 punct ", ",cut
R4013 T6318 T6319 amod thin,sections
R4014 T6319 T6313 nsubjpass sections,cut
R4015 T6320 T6313 auxpass were,cut
R4016 T6321 T6313 advcl using,cut
R4017 T6322 T6323 det an,ultramicrotome
R4018 T6323 T6321 dobj ultramicrotome,using
R4019 T6324 T6313 punct ", ",cut
R4020 T6325 T6313 conj stained,cut
R4021 T6326 T6325 prep with,stained
R4022 T6327 T6328 nummod 1.5,%
R4023 T6328 T6329 compound %,uranylacetate
R4024 T6329 T6326 pobj uranylacetate,with
R4025 T6330 T6325 prep in,stained
R4026 T6331 T6332 nummod 50,%
R4027 T6332 T6333 compound %,ethanol
R4028 T6333 T6330 pobj ethanol,in
R4029 T6334 T6333 cc and,ethanol
R4030 T6335 T6336 nummod 0.8,%
R4031 T6336 T6337 compound %,citrate
R4032 T6337 T6333 conj citrate,ethanol
R4033 T6338 T6337 compound lead,citrate
R4034 T6339 T6325 punct ", ",stained
R4035 T6340 T6325 cc and,stained
R4036 T6341 T6325 conj analysed,stained
R4037 T6342 T6341 advcl using,analysed
R4038 T6343 T6344 compound electron,microscope
R4039 T6344 T6342 dobj microscope,using
R4040 T6345 T6313 punct .,cut
R4041 T6347 T6348 det All,procedures
R4042 T6348 T6349 nsubjpass procedures,conducted
R4043 T6350 T6349 auxpass were,conducted
R4044 T6351 T6349 prep according,conducted
R4045 T6352 T6351 prep to,according
R4046 T6353 T6354 det the,Committee
R4047 T6354 T6358 poss Committee,guidelines
R4048 T6355 T6354 compound Eisai,Committee
R4049 T6356 T6357 compound Animal,Care
R4050 T6357 T6354 compound Care,Committee
R4051 T6358 T6352 pobj guidelines,to
R4052 T6359 T6354 case 's,Committee
R4053 T6360 T6349 punct .,conducted
R4054 T6427 T6428 amod Frozen,sections
R4055 T6428 T6429 nsubjpass sections,rinsed
R4056 T6430 T6429 auxpass were,rinsed
R4057 T6431 T6429 prep in,rinsed
R4058 T6432 T6433 nummod 0.1,%
R4059 T6433 T6431 pobj %,in
R4060 T6434 T6435 compound Triton,X
R4061 T6435 T6433 appos X,%
R4062 T6436 T6435 punct -,X
R4063 T6437 T6435 nummod 100,X
R4064 T6438 T6433 punct /,%
R4065 T6439 T6433 appos PBS,%
R4066 T6440 T6429 prep at,rinsed
R4067 T6441 T6442 compound room,temperature
R4068 T6442 T6440 pobj temperature,at
R4069 T6443 T6429 prep for,rinsed
R4070 T6444 T6445 nummod 1,hour
R4071 T6445 T6443 pobj hour,for
R4072 T6446 T6429 punct ", ",rinsed
R4073 T6447 T6429 cc and,rinsed
R4074 T6448 T6449 auxpass were,blocked
R4075 T6449 T6429 conj blocked,rinsed
R4076 T6450 T6449 prep in,blocked
R4077 T6451 T6452 compound BLOCKACE,solution
R4078 T6452 T6450 pobj solution,in
R4079 T6453 T6454 punct (,nippon
R4080 T6454 T6452 parataxis nippon,solution
R4081 T6455 T6454 compound Dai,nippon
R4082 T6456 T6454 punct -,nippon
R4083 T6457 T6454 punct ),nippon
R4084 T6458 T6429 punct .,rinsed
R4085 T6460 T6461 det The,antibodies
R4086 T6461 T6463 nsubjpass antibodies,incubated
R4087 T6462 T6461 amod following,antibodies
R4088 T6464 T6463 auxpass were,incubated
R4089 T6465 T6463 advmod overnight,incubated
R4090 T6466 T6463 prep in,incubated
R4091 T6467 T6468 nummod 0.1,%
R4092 T6468 T6469 compound %,BLOCKACE
R4093 T6469 T6466 pobj BLOCKACE,in
R4094 T6470 T6463 prep at,incubated
R4095 T6471 T6472 nummod 4,°C
R4096 T6472 T6470 pobj °C,at
R4097 T6473 T6463 punct :,incubated
R4098 T6475 T6476 amod monoclonal,K
R4099 T6477 T6476 nmod mouse,K
R4100 T6478 T6476 amod anti-calbindin,K
R4101 T6479 T6476 nummod 28,K
R4102 T6480 T6481 punct (,200
R4103 T6481 T6476 parataxis 200,K
R4104 T6482 T6481 dep Sigma,200
R4105 T6483 T6481 punct ", ",200
R4106 T6484 T6481 nummod 1,200
R4107 T6485 T6481 punct :,200
R4108 T6486 T6481 punct ),200
R4109 T6487 T6476 punct ;,K
R4110 T6488 T6489 amod monoclonal,mouse
R4111 T6489 T6476 appos mouse,K
R4112 T6490 T6489 amod anti-MBP,mouse
R4113 T6491 T6492 punct (,50
R4114 T6492 T6489 parataxis 50,mouse
R4115 T6493 T6492 dep SMI,50
R4116 T6494 T6493 punct -,SMI
R4117 T6495 T6493 nummod 99,SMI
R4118 T6496 T6492 punct ", ",50
R4119 T6497 T6498 compound Sternberger,Monoclonals
R4120 T6498 T6492 dep Monoclonals,50
R4121 T6499 T6498 amod Inc.,Monoclonals
R4122 T6500 T6492 punct ", ",50
R4123 T6501 T6492 quantmod 1,50
R4124 T6502 T6492 punct :,50
R4125 T6503 T6492 punct ),50
R4126 T6504 T6476 punct ;,K
R4127 T6505 T6506 amod monoclonal,N
R4128 T6506 T6476 appos N,K
R4129 T6507 T6506 nmod mouse,N
R4130 T6508 T6506 amod anti-Neu,N
R4131 T6509 T6510 punct (,100
R4132 T6510 T6506 parataxis 100,N
R4133 T6511 T6510 dep CHEMICON,100
R4134 T6512 T6510 punct ", ",100
R4135 T6513 T6510 quantmod 1,100
R4136 T6514 T6510 punct :,100
R4137 T6515 T6510 punct ),100
R4138 T6516 T6476 punct ;,K
R4139 T6517 T6476 appos rabbit,K
R4140 T6518 T6517 amod anti-S100,rabbit
R4141 T6519 T6517 amod polyclonal,rabbit
R4142 T6520 T6521 punct (,200
R4143 T6521 T6517 parataxis 200,rabbit
R4144 T6522 T6521 dep DAKO,200
R4145 T6523 T6521 punct ", ",200
R4146 T6524 T6521 quantmod 1,200
R4147 T6525 T6521 punct :,200
R4148 T6526 T6521 punct ),200
R4149 T6527 T6476 punct .,K
R4150 T6529 T6530 nsubjpass Nuclei,counterstained
R4151 T6531 T6530 auxpass were,counterstained
R4152 T6532 T6530 prep with,counterstained
R4153 T6533 T6534 nummod 1,μg
R4154 T6534 T6535 nmod μg,DAPI
R4155 T6535 T6532 pobj DAPI,with
R4156 T6536 T6537 punct /,ml
R4157 T6537 T6534 prep ml,μg
R4158 T6538 T6539 punct (,Sigma
R4159 T6539 T6535 parataxis Sigma,DAPI
R4160 T6540 T6539 punct ),Sigma
R4161 T6541 T6530 punct .,counterstained
R4162 T6543 T6544 compound MBP,staining
R4163 T6544 T6545 nsubjpass staining,performed
R4164 T6546 T6545 auxpass was,performed
R4165 T6547 T6545 prep after,performed
R4166 T6548 T6549 compound microwave,retrieval
R4167 T6549 T6547 pobj retrieval,after
R4168 T6550 T6549 compound epitope,retrieval
R4169 T6551 T6549 prep in,retrieval
R4170 T6552 T6553 nummod 10,mmol
R4171 T6553 T6554 nmod mmol,buffer
R4172 T6554 T6551 pobj buffer,in
R4173 T6555 T6556 punct /,L
R4174 T6556 T6553 prep L,mmol
R4175 T6557 T6554 compound citrate,buffer
R4176 T6558 T6554 punct (,buffer
R4177 T6559 T6554 npadvmod pH,buffer
R4178 T6560 T6559 nummod 6,pH
R4179 T6561 T6554 punct ),buffer
R4180 T6562 T6545 punct .,performed
R4181 T6564 T6565 nsubjpass Sections,incubated
R4182 T6566 T6565 auxpass were,incubated
R4183 T6567 T6565 prep for,incubated
R4184 T6568 T6569 nummod 1,hour
R4185 T6569 T6567 pobj hour,for
R4186 T6570 T6565 prep with,incubated
R4187 T6571 T6572 amod secondary,antibodies
R4188 T6572 T6570 pobj antibodies,with
R4189 T6573 T6572 punct : ,antibodies
R4190 T6574 T6575 npadvmod Cy3,labeled
R4191 T6575 T6577 amod labeled,IgG
R4192 T6576 T6575 punct -,labeled
R4193 T6577 T6572 appos IgG,antibodies
R4194 T6578 T6577 nmod donkey,IgG
R4195 T6579 T6577 amod anti-rabbit,IgG
R4196 T6580 T6581 punct (,200
R4197 T6581 T6577 parataxis 200,IgG
R4198 T6582 T6583 compound Jackson,Research
R4199 T6583 T6581 dep Research,200
R4200 T6584 T6583 compound Immuno,Research
R4201 T6585 T6581 punct ", ",200
R4202 T6586 T6581 quantmod 1,200
R4203 T6587 T6581 punct :,200
R4204 T6588 T6581 punct ),200
R4205 T6589 T6577 punct ", ",IgG
R4206 T6590 T6591 npadvmod FITC,labelled
R4207 T6591 T6593 amod labelled,IgG
R4208 T6592 T6591 punct -,labelled
R4209 T6593 T6577 appos IgG,IgG
R4210 T6594 T6593 nmod donkey,IgG
R4211 T6595 T6593 amod anti-mouse,IgG
R4212 T6596 T6597 punct (,200
R4213 T6597 T6593 parataxis 200,IgG
R4214 T6598 T6599 compound Jackson,Research
R4215 T6599 T6597 dep Research,200
R4216 T6600 T6599 compound Immuno,Research
R4217 T6601 T6597 punct ", ",200
R4218 T6602 T6597 quantmod 1,200
R4219 T6603 T6597 punct :,200
R4220 T6604 T6597 punct ),200
R4221 T6605 T6565 punct .,incubated
R4222 T6607 T6608 nsubjpass Sections,photographed
R4223 T6609 T6608 auxpass were,photographed
R4224 T6610 T6608 prep with,photographed
R4225 T6611 T6612 det an,microscope
R4226 T6612 T6610 pobj microscope,with
R4227 T6613 T6612 compound Olympus,microscope
R4228 T6614 T6615 punct (,IX71
R4229 T6615 T6612 parataxis IX71,microscope
R4230 T6616 T6615 compound Olympus,IX71
R4231 T6617 T6615 punct ),IX71
R4232 T6618 T6612 acl equipped,microscope
R4233 T6619 T6618 prep with,equipped
R4234 T6620 T6621 det a,camera
R4235 T6621 T6619 pobj camera,with
R4236 T6622 T6623 amod high,resolution
R4237 T6623 T6621 compound resolution,camera
R4238 T6624 T6623 punct -,resolution
R4239 T6625 T6621 compound CCD,camera
R4240 T6626 T6608 punct .,photographed
R4241 T6692 T6693 nmod RNA,hybridisation
R4242 T6694 T6695 advmod in,situ
R4243 T6695 T6693 amod situ,hybridisation
R4244 T6697 T6698 advmod In,situ
R4245 T6698 T6699 amod situ,hybridisation
R4246 T6699 T6700 nsubjpass hybridisation,carried
R4247 T6701 T6699 prep on,hybridisation
R4248 T6702 T6703 amod frozen,sections
R4249 T6703 T6701 pobj sections,on
R4250 T6704 T6700 auxpass were,carried
R4251 T6705 T6700 prt out,carried
R4252 T6706 T6700 advcl using,carried
R4253 T6707 T6708 advmod 35S,labelled
R4254 T6708 T6710 amod labelled,probes
R4255 T6709 T6708 punct -,labelled
R4256 T6710 T6706 dobj probes,using
R4257 T6711 T6710 compound RNA,probes
R4258 T6712 T6700 punct .,carried
R4259 T6714 T6715 advmod Briefly,cloned
R4260 T6716 T6715 punct ", ",cloned
R4261 T6717 T6718 nummod 610,bp
R4262 T6718 T6715 nsubjpass bp,cloned
R4263 T6719 T6718 prep of,bp
R4264 T6720 T6721 compound mouse,cDNA
R4265 T6721 T6719 pobj cDNA,of
R4266 T6722 T6721 compound ADAM22,cDNA
R4267 T6723 T6724 punct (,1960
R4268 T6724 T6721 parataxis 1960,cDNA
R4269 T6725 T6724 dep position,1960
R4270 T6726 T6724 punct : ,1960
R4271 T6727 T6724 nummod 1351,1960
R4272 T6728 T6724 punct –,1960
R4273 T6729 T6724 prep from,1960
R4274 T6730 T6731 amod initiating,ATG
R4275 T6731 T6729 pobj ATG,from
R4276 T6732 T6724 punct ),1960
R4277 T6733 T6715 auxpass was,cloned
R4278 T6734 T6715 prep into,cloned
R4279 T6735 T6736 det the,SK
R4280 T6736 T6734 pobj SK,into
R4281 T6737 T6736 nmod pBluescript,SK
R4282 T6738 T6736 nummod II,SK
R4283 T6739 T6740 punct (,+
R4284 T6740 T6736 punct +,SK
R4285 T6741 T6740 punct ),+
R4286 T6742 T6736 cc or,SK
R4287 T6743 T6744 det the,KS
R4288 T6744 T6747 nmod KS,vector
R4289 T6745 T6744 nmod pBluescript,KS
R4290 T6746 T6744 nummod II,KS
R4291 T6747 T6736 conj vector,SK
R4292 T6748 T6749 punct (,+
R4293 T6749 T6744 punct +,KS
R4294 T6750 T6749 punct ),+
R4295 T6751 T6715 punct .,cloned
R4296 T6753 T6754 advcl Using,generated
R4297 T6755 T6756 det these,plasmids
R4298 T6756 T6753 dobj plasmids,Using
R4299 T6757 T6753 prep as,Using
R4300 T6758 T6757 pobj templates,as
R4301 T6759 T6754 punct ", ",generated
R4302 T6760 T6761 npadvmod sense,labelled
R4303 T6761 T6764 amod labelled,probes
R4304 T6762 T6760 cc and,sense
R4305 T6763 T6760 conj antisense,sense
R4306 T6764 T6754 nsubjpass probes,generated
R4307 T6765 T6764 compound RNA,probes
R4308 T6766 T6754 auxpass were,generated
R4309 T6767 T6754 agent by,generated
R4310 T6768 T6769 compound T7,polymerase
R4311 T6769 T6767 pobj polymerase,by
R4312 T6770 T6769 compound RNA,polymerase
R4313 T6771 T6769 cc and,polymerase
R4314 T6772 T6773 punct [,UTP
R4315 T6773 T6769 conj UTP,polymerase
R4316 T6774 T6773 nmod α35S,UTP
R4317 T6775 T6773 punct ],UTP
R4318 T6776 T6754 punct .,generated
R4319 T6778 T6779 amod Frozen,brain
R4320 T6779 T6780 nsubjpass brain,used
R4321 T6781 T6779 cc and,brain
R4322 T6782 T6783 amod spinal,cord
R4323 T6783 T6779 conj cord,brain
R4324 T6784 T6779 prep from,brain
R4325 T6785 T6786 nummod 2,month
R4326 T6786 T6787 npadvmod month,old
R4327 T6787 T6789 amod old,mice
R4328 T6788 T6787 punct -,old
R4329 T6789 T6784 pobj mice,from
R4330 T6790 T6789 nmod C57BL,mice
R4331 T6791 T6790 punct /,C57BL
R4332 T6792 T6790 nummod 6,C57BL
R4333 T6793 T6789 amod female,mice
R4334 T6794 T6780 auxpass were,used
R4335 T6795 T6780 prep in,used
R4336 T6796 T6797 det this,analysis
R4337 T6797 T6795 pobj analysis,in
R4338 T6798 T6780 punct .,used
R4339 T6800 T6801 nsubjpass Pretreatment,carried
R4340 T6802 T6800 punct ", ",Pretreatment
R4341 T6803 T6800 conj hybridisation,Pretreatment
R4342 T6804 T6803 punct ", ",hybridisation
R4343 T6805 T6806 compound RNase,treatment
R4344 T6806 T6803 conj treatment,hybridisation
R4345 T6807 T6806 cc and,treatment
R4346 T6808 T6806 conj washing,treatment
R4347 T6809 T6801 auxpass was,carried
R4348 T6810 T6801 prt out,carried
R4349 T6811 T6801 advcl following,carried
R4350 T6812 T6813 det the,protocol
R4351 T6813 T6811 dobj protocol,following
R4352 T6814 T6813 acl described,protocol
R4353 T6815 T6814 prep in,described
R4354 T6816 T6817 det the,literature
R4355 T6817 T6815 pobj literature,in
R4356 T6818 T6819 punct [,33
R4357 T6819 T6801 parataxis 33,carried
R4358 T6820 T6819 punct ],33
R4359 T6821 T6801 punct .,carried
R4360 T6823 T6824 amod Dehydrated,slides
R4361 T6824 T6825 nsubjpass slides,attached
R4362 T6826 T6825 auxpass were,attached
R4363 T6827 T6825 prep to,attached
R4364 T6828 T6829 compound imaging,plates
R4365 T6829 T6827 pobj plates,to
R4366 T6830 T6825 prep for,attached
R4367 T6831 T6832 nummod 48,hours
R4368 T6832 T6830 pobj hours,for
R4369 T6833 T6825 cc and,attached
R4370 T6834 T6835 det the,autoradiograms
R4371 T6835 T6836 nsubjpass autoradiograms,analysed
R4372 T6836 T6825 conj analysed,attached
R4373 T6837 T6836 auxpass were,analysed
R4374 T6838 T6836 advcl using,analysed
R4375 T6839 T6840 det a,Analyser
R4376 T6840 T6838 dobj Analyser,using
R4377 T6841 T6840 compound Bio-Image,Analyser
R4378 T6842 T6843 punct (,Film
R4379 T6843 T6840 parataxis Film,Analyser
R4380 T6844 T6843 dep BAS3000,Film
R4381 T6845 T6843 punct ", ",Film
R4382 T6846 T6843 compound Fuji,Film
R4383 T6847 T6843 compound Photo,Film
R4384 T6848 T6843 punct ),Film
R4385 T6849 T6836 punct .,analysed
R1 T87 T85 cc and,Ataxia
R2 T88 T89 amod peripheral,hypomyelination
R3 T90 T89 compound nerve,hypomyelination
R7 T94 T93 punct -,deficient
R15 T107 T106 compound ADAM,family
R16 T108 T106 compound gene,family
R22 T114 T115 mark that,expressed
R26 T118 T119 advmod only,in
R27 T119 T115 prep in,expressed
R28 T120 T121 det the,systems
R36 T129 T127 case 's,ADAM22
R37 T130 T128 compound sequence,similarity
R43 T137 T136 aux to,be
R46 T140 T139 compound integrin,binder
R50 T144 T143 aux to,have
R56 T150 T149 punct -,cell
R58 T152 T149 cc or,cell
R59 T153 T154 compound cell,matrix
R67 T163 T162 amod physiological,functions
R82 T181 T180 punct -,deficient
R88 T188 T187 amod good,accordance
R92 T192 T191 amod Mendelian,ratio
R121 T224 T223 amod major,abnormalities
R122 T225 T223 amod histological,abnormalities
R124 T227 T226 auxpass were,detected
R128 T232 T231 amod cerebral,cortex
R134 T238 T237 amod homozygous,mutants
R143 T247 T246 amod peripheral,nerves
R153 T261 T260 nsubjpass ADAM22,involved
R154 T262 T260 auxpass is,involved
R155 T263 T260 advmod closely,involved
R159 T267 T266 amod correct,functioning
R163 T271 T270 amod nervous,system
R718 T1301 T1291 nsubjpass form,synthesized
R818 T1407 T1403 pobj littermates,from
R889 T1484 T1482 pobj females,to
R983 T1588 T1584 nsubj weight,was
R996 T1601 T1597 pobj littermates,of
R187 T593 T594 nsubj ADAM,is
R188 T595 T593 punct (,ADAM
R189 T596 T597 det A,Disintegrin
R190 T597 T593 appos Disintegrin,ADAM
R191 T598 T597 cc And,Disintegrin
R192 T599 T597 conj Metalloprotease,Disintegrin
R193 T600 T594 punct ),is
R194 T601 T602 det a,family
R195 T602 T594 attr family,is
R196 T603 T602 prep of,family
R197 T604 T605 npadvmod membrane,spanning
R202 T609 T607 acl containing,proteins
R203 T610 T611 det a,domain
R206 T613 T611 amod like,domain
R208 T615 T611 cc and,domain
R209 T616 T617 det a,domain
R212 T619 T617 amod like,domain
R214 T621 T594 punct .,is
R215 T623 T624 advmod Currently,identified
R216 T625 T624 punct ", ",identified
R217 T626 T627 amod more,30
R220 T629 T624 nsubjpass ADAMs,identified
R221 T630 T624 aux have,identified
R222 T631 T624 auxpass been,identified
R223 T632 T624 prep in,identified
R224 T633 T632 pobj mammals,in
R225 T634 T624 punct .,identified
R226 T636 T637 poss Their,activities
R229 T640 T639 dobj ADAMs,implicate
R230 T641 T639 prep in,implicate
R231 T642 T641 pobj fertilization,in
R232 T643 T642 punct ", ",fertilization
R233 T644 T642 conj myogenesis,fertilization
R234 T645 T644 cc and,myogenesis
R235 T646 T644 conj neurogenesis,myogenesis
R236 T647 T642 prep by,fertilization
R237 T648 T647 pobj proteolysis,by
R238 T649 T648 cc and,proteolysis
R239 T650 T648 conj adhesion,proteolysis
R240 T651 T639 punct .,implicate
R241 T653 T654 det Some,types
R242 T654 T655 nsubj types,are
R243 T656 T654 prep of,types
R244 T657 T656 pobj ADAM,of
R245 T658 T659 advmod catalytically,active
R246 T659 T660 amod active,metalloproteases
R247 T660 T655 attr metalloproteases,are
R248 T661 T655 cc and,are
R249 T662 T655 conj shed,are
R250 T663 T664 det the,domains
R253 T666 T664 prep of,domains
R254 T667 T668 npadvmod membrane,bound
R259 T672 T670 cc or,factors
R260 T673 T670 conj receptors,factors
R261 T674 T675 punct [,2
R265 T678 T675 punct ],2
R266 T679 T655 punct .,are
R267 T681 T682 prep For,shown
R268 T683 T681 pobj example,For
R269 T684 T682 punct ", ",shown
R270 T685 T682 nsubjpass ADAM17,shown
R271 T686 T687 punct (,TACE
R272 T687 T685 parataxis TACE,ADAM17
R273 T688 T687 punct ),TACE
R274 T689 T682 aux has,shown
R275 T690 T682 auxpass been,shown
R276 T691 T692 aux to,cleave
R277 T692 T682 xcomp cleave,shown
R278 T693 T694 amod several,substrates
R279 T694 T692 dobj substrates,cleave
R280 T695 T694 punct ", ",substrates
R281 T696 T694 prep including,substrates
R282 T697 T698 compound tumour,alpha
R286 T701 T702 punct [,4
R290 T705 T702 punct ],4
R291 T706 T698 punct ", ",alpha
R292 T707 T708 npadvmod heparin,binding
R297 T712 T714 npadvmod factor,like
R299 T714 T710 amod like,factor
R302 T717 T718 punct [,7
R306 T721 T718 punct ],7
R307 T722 T710 cc and,factor
R308 T723 T724 amod transforming,alpha
R312 T727 T728 punct [,8
R313 T728 T682 parataxis 8,shown
R314 T729 T728 punct ],8
R315 T730 T682 punct .,shown
R316 T732 T733 nsubj Studies,revealed
R317 T734 T732 prep of,Studies
R318 T735 T736 npadvmod ADAM17,null
R321 T738 T734 pobj mice,of
R322 T739 T733 aux have,revealed
R323 T740 T741 mark that,is
R326 T743 T741 acomp critical,is
R327 T744 T741 prep in,is
R328 T745 T744 pobj embryogenesis,in
R329 T746 T741 cc and,is
R330 T747 T741 conj plays,is
R331 T748 T749 det an,role
R334 T751 T747 prep in,plays
R335 T752 T753 det the,supply
R336 T753 T751 pobj supply,in
R337 T754 T753 prep of,supply
R338 T755 T756 compound growth,factors
R339 T756 T754 pobj factors,of
R340 T757 T758 punct [,8
R344 T761 T758 punct ],8
R345 T762 T733 punct .,revealed
R346 T764 T765 nsubjpass ADAMs,involved
R347 T766 T765 auxpass are,involved
R348 T767 T765 advmod also,involved
R349 T768 T765 prep in,involved
R350 T769 T770 nmod cell,cell
R356 T775 T770 conj matrix,cell
R358 T777 T765 prep through,involved
R359 T778 T779 poss their,interaction
R360 T779 T777 pobj interaction,through
R361 T780 T779 prep with,interaction
R362 T781 T780 pobj integrins,with
R363 T782 T781 cc or,integrins
R364 T783 T781 conj syndecans,integrins
R365 T784 T765 punct .,involved
R366 T786 T787 amod More,10
R369 T789 T790 nsubjpass ADAMs,shown
R370 T791 T790 aux have,shown
R371 T792 T790 auxpass been,shown
R372 T793 T794 aux to,support
R373 T794 T790 xcomp support,shown
R374 T795 T796 npadvmod integrin,mediated
R379 T800 T801 advmod in,vitro
R380 T801 T794 advmod vitro,support
R381 T802 T803 punct [,9
R382 T803 T790 parataxis 9,shown
R383 T804 T803 punct ],9
R384 T805 T790 punct .,shown
R385 T807 T808 nsubjpass It,reported
R386 T809 T808 aux has,reported
R387 T810 T808 auxpass been,reported
R388 T811 T812 mark that,are
R391 T814 T816 amod null,mutants
R399 T822 T812 acomp infertile,are
R400 T823 T812 cc and,are
R401 T824 T825 poss their,spermatozoa
R402 T825 T826 nsubj spermatozoa,lack
R403 T826 T812 conj lack,are
R404 T827 T828 npadvmod egg,binding
R407 T830 T826 dobj abilities,lack
R408 T831 T832 punct [,10
R409 T832 T808 parataxis 10,reported
R410 T833 T834 punct -,12
R411 T834 T832 prep 12,10
R412 T835 T832 punct ],10
R413 T836 T808 punct .,reported
R414 T838 T839 preconj Both,ADAM2
R415 T839 T840 nsubj ADAM2,are
R416 T841 T839 cc and,ADAM2
R417 T842 T839 conj ADAM3,ADAM2
R418 T843 T840 neg not,are
R419 T844 T840 attr metalloproteases,are
R420 T845 T846 mark because,lack
R423 T848 T849 amod catalytic,sequences
R426 T851 T846 prep in,lack
R427 T852 T853 poss their,domain
R430 T855 T840 punct .,are
R431 T857 T858 det These,studies
R432 T858 T859 nsubj studies,showed
R433 T860 T859 advmod clearly,showed
R434 T861 T862 mark that,have
R441 T868 T869 amod significant,roles
R442 T869 T862 dobj roles,have
R443 T870 T871 advmod in,vivo
R444 T871 T862 advmod vivo,have
R445 T872 T859 punct .,showed
R446 T874 T875 nsubj We,reported
R447 T876 T875 aux have,reported
R448 T877 T878 det the,findings
R449 T878 T875 dobj findings,reported
R450 T879 T878 prep of,findings
R451 T880 T881 nmod ADAM11,genes
R457 T886 T878 cc and,findings
R458 T887 T888 poss their,expression
R461 T890 T888 prep in,expression
R462 T891 T892 det the,systems
R468 T897 T898 punct [,13
R469 T898 T875 parataxis 13,reported
R470 T899 T900 punct -,15
R471 T900 T898 prep 15,13
R472 T901 T898 punct ],13
R473 T902 T875 punct .,reported
R474 T904 T905 compound Sequence,analysis
R475 T905 T906 nsubj analysis,suggests
R476 T907 T908 mark that,are
R479 T910 T908 neg not,are
R480 T911 T908 attr metalloproteases,are
R481 T912 T906 punct ", ",suggests
R482 T913 T914 mark since,lack
R486 T917 T918 det a,motif
R489 T920 T906 punct .,suggests
R490 T922 T923 nsubjpass It,reported
R491 T924 T923 aux has,reported
R492 T925 T923 auxpass been,reported
R493 T926 T927 mark that,localised
R498 T931 T927 prep to,localised
R499 T932 T933 det the,surface
R502 T935 T936 punct [,16
R503 T936 T927 parataxis 16,localised
R504 T937 T936 punct ],16
R505 T938 T927 punct ", ",localised
R506 T939 T927 dep interacts,localised
R507 T940 T939 prep with,interacts
R508 T941 T942 nmod alpha,v
R516 T949 T950 punct [,17
R517 T950 T939 parataxis 17,interacts
R518 T951 T950 punct ],17
R519 T952 T927 cc and,localised
R520 T953 T954 det the,disruption
R521 T954 T955 nsubj disruption,results
R529 T962 T955 prep in,results
R530 T963 T964 amod premature,death
R531 T964 T962 pobj death,in
R532 T965 T964 acl associated,death
R533 T966 T965 prep with,associated
R534 T967 T966 pobj ataxia,with
R535 T968 T967 cc and,ataxia
R536 T969 T967 conj tremor,ataxia
R537 T970 T971 punct [,18
R538 T971 T955 parataxis 18,results
R539 T972 T971 punct ],18
R540 T973 T923 punct .,reported
R541 T975 T976 mark Although,is
R550 T985 T976 acomp unknown,is
R551 T986 T984 punct ", ",be
R552 T987 T984 prep for,be
R553 T988 T989 det these,phenotypes
R554 T989 T987 pobj phenotypes,for
R555 T990 T984 punct ", ",be
R556 T991 T992 amod impaired,interactions
R559 T994 T992 nmod cell,interactions
R563 T998 T994 conj matrix,cell
R565 T1000 T992 prep in,interactions
R566 T1001 T1002 det the,system
R569 T1004 T992 acl caused,interactions
R570 T1005 T1004 agent by,caused
R571 T1006 T1005 pobj loss,by
R572 T1007 T1006 prep of,loss
R573 T1008 T1007 pobj ADAM23,of
R574 T1009 T984 aux may,be
R575 T1010 T984 acomp responsible,be
R576 T1011 T984 punct .,be
R577 T1013 T1014 nsubj ADAM22,share
R578 T1015 T1013 cc and,ADAM22
R579 T1016 T1013 conj ADAM23,ADAM22
R580 T1017 T1018 advmod highly,homologous
R581 T1018 T1019 amod homologous,sequences
R582 T1019 T1014 dobj sequences,share
R583 T1020 T1014 prep in,share
R584 T1021 T1022 poss their,domains
R587 T1024 T1014 punct .,share
R588 T1026 T1027 advmod Especially,is
R589 T1028 T1027 punct ", ",is
R590 T1029 T1027 nsubj it,is
R591 T1030 T1027 acomp evident,is
R592 T1031 T1027 prep in,is
R593 T1032 T1033 poss their,sequences
R599 T1038 T1033 punct ", ",sequences
R600 T1039 T1040 nmod CR,CD
R613 T1052 T1040 punct ", ",CD
R614 T1053 T1054 dep which,located
R617 T1056 T1054 advmod centre,located
R618 T1057 T1056 prep of,centre
R619 T1058 T1059 det the,domain
R622 T1061 T1027 punct .,is
R623 T1063 T1064 det These,findings
R624 T1064 T1065 nsubj findings,led
R625 T1066 T1065 dobj us,led
R626 T1067 T1068 aux to,hypothesize
R627 T1068 T1065 xcomp hypothesize,led
R628 T1069 T1070 mark that,is
R631 T1072 T1073 det an,binder
R634 T1075 T1070 cc and,is
R635 T1076 T1070 conj plays,is
R636 T1077 T1078 det an,role
R639 T1080 T1076 prep in,plays
R640 T1081 T1082 det the,system
R643 T1084 T1076 punct ", ",plays
R644 T1085 T1086 mark as,does
R645 T1086 T1076 advcl does,plays
R646 T1087 T1086 nsubj ADAM23,does
R647 T1088 T1065 punct .,led
R648 T1090 T1091 aux To,determine
R649 T1091 T1092 advcl determine,generated
R650 T1093 T1094 det the,functions
R653 T1096 T1094 prep of,functions
R654 T1097 T1096 pobj ADAM22,of
R655 T1098 T1092 punct ", ",generated
R656 T1099 T1092 nsubj we,generated
R657 T1100 T1092 cc and,generated
R658 T1101 T1092 conj analysed,generated
R659 T1102 T1103 nmod Adam22,mice
R662 T1105 T1103 amod targeted,mice
R664 T1107 T1092 punct .,generated
R1182 T2098 T2096 ccomp is,suggest