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PMC:1140370 / 9639-10893 JSONTXT

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Id Subject Object Predicate Lexical cue
T2971 0-3 DT denotes The
T2972 13-21 NNS denotes mixtures
T2973 4-12 NN denotes reaction
T2974 30-39 VBD denotes contained
T2975 22-23 -LRB- denotes (
T2976 26-28 NN denotes μl
T2977 23-25 CD denotes 25
T2978 28-29 -RRB- denotes )
T2979 40-42 CD denotes 25
T2980 43-45 NN denotes mM
T2981 52-56 NN denotes NaOH
T2982 46-51 NN denotes HEPES
T2983 51-52 HYPH denotes –
T2984 57-58 -LRB- denotes (
T2985 58-60 NN denotes pH
T2986 61-64 CD denotes 7.5
T2987 64-65 -RRB- denotes )
T2988 65-67 , denotes ,
T2989 67-69 CD denotes 50
T2990 70-72 NN denotes mM
T2991 80-87 NN denotes acetate
T2992 73-79 NN denotes sodium
T2993 87-89 , denotes ,
T2994 89-91 CD denotes 10
T2995 92-94 NN denotes mM
T2996 98-104 NN denotes CH3COO
T2997 95-97 NN denotes Mg
T2998 97-98 -LRB- denotes (
T2999 104-105 -RRB- denotes )
T3000 105-106 CD denotes 2
T3001 106-108 , denotes ,
T3002 108-109 CD denotes 1
T3003 110-112 NN denotes mM
T3004 113-116 NN denotes DTT
T3005 116-118 , denotes ,
T3006 118-122 CD denotes 0.25
T3007 123-125 NN denotes mg
T3008 129-132 NN denotes BSA
T3009 125-126 SYM denotes /
T3010 126-128 NN denotes ml
T3011 132-134 , denotes ,
T3012 134-137 CD denotes 0.5
T3013 138-140 NN denotes mM
T3014 147-148 NN denotes S
T3015 141-144 NN denotes ATP
T3016 144-145 HYPH denotes -
T3017 145-146 SYM denotes γ
T3018 146-147 HYPH denotes -
T3019 148-150 , denotes ,
T3020 150-153 CD denotes 0.5
T3021 154-156 NN denotes mM
T3022 157-162 NN denotes CaCl2
T3023 162-164 , denotes ,
T3024 164-166 CD denotes 10
T3025 167-171 NN denotes fmol
T3026 172-174 IN denotes of
T3027 175-178 NN denotes 32P
T3028 179-186 VBN denotes labeled
T3029 178-179 HYPH denotes -
T3030 194-203 NN denotes substrate
T3031 187-193 NN denotes bubble
T3032 203-205 , denotes ,
T3033 205-208 CC denotes and
T3034 209-212 DT denotes the
T3035 223-229 NN denotes amount
T3036 213-222 VBN denotes indicated
T3037 230-232 IN denotes of
T3038 233-237 NN denotes Mcm4
T3039 242-250 NN denotes proteins
T3040 237-238 HYPH denotes /
T3041 238-239 CD denotes 6
T3042 239-240 HYPH denotes /
T3043 240-241 CD denotes 7
T3044 250-251 . denotes .
T3045 251-421 sentence denotes After incubation at 30°C for 30 min, the indicated amount of DNase I (TAKARA Biomedical, Japan) was added, and the mixtures were incubated at room temperature for 1 min.
T3046 252-257 IN denotes After
T3047 352-357 VBN denotes added
T3048 258-268 NN denotes incubation
T3049 269-271 IN denotes at
T3050 272-274 CD denotes 30
T3051 274-276 NN denotes °C
T3052 277-280 IN denotes for
T3053 281-283 CD denotes 30
T3054 284-287 NN denotes min
T3055 287-289 , denotes ,
T3056 289-292 DT denotes the
T3057 303-309 NN denotes amount
T3058 293-302 VBN denotes indicated
T3059 310-312 IN denotes of
T3060 313-318 NN denotes DNase
T3061 319-320 CD denotes I
T3062 321-322 -LRB- denotes (
T3063 329-339 NNP denotes Biomedical
T3064 322-328 NNP denotes TAKARA
T3065 339-341 , denotes ,
T3066 341-346 NNP denotes Japan
T3067 346-347 -RRB- denotes )
T3068 348-351 VBD denotes was
T3069 357-359 , denotes ,
T3070 359-362 CC denotes and
T3071 363-366 DT denotes the
T3072 367-375 NNS denotes mixtures
T3073 381-390 VBN denotes incubated
T3074 376-380 VBD denotes were
T3075 391-393 IN denotes at
T3076 394-398 NN denotes room
T3077 399-410 NN denotes temperature
T3078 411-414 IN denotes for
T3079 415-416 CD denotes 1
T3080 417-420 NN denotes min
T3081 420-421 . denotes .
T3082 421-557 sentence denotes DNase I was inactivated by the addition of 250 μl of stop solution (20 mM EDTA, 0.2M sodium chloride, 1% SDS and 12.5 μg/ml yeast RNA).
T3083 422-427 NN denotes DNase
T3084 434-445 VBN denotes inactivated
T3085 428-429 CD denotes I
T3086 430-433 VBD denotes was
T3087 446-448 IN denotes by
T3088 449-452 DT denotes the
T3089 453-461 NN denotes addition
T3090 462-464 IN denotes of
T3091 465-468 CD denotes 250
T3092 469-471 NN denotes μl
T3093 472-474 IN denotes of
T3094 475-479 NN denotes stop
T3095 480-488 NN denotes solution
T3096 489-490 -LRB- denotes (
T3097 496-500 NN denotes EDTA
T3098 490-492 CD denotes 20
T3099 493-495 NN denotes mM
T3100 500-502 , denotes ,
T3101 502-506 NN denotes 0.2M
T3102 514-522 NN denotes chloride
T3103 507-513 NN denotes sodium
T3104 522-524 , denotes ,
T3105 524-525 CD denotes 1
T3106 525-526 NN denotes %
T3107 527-530 NN denotes SDS
T3108 531-534 CC denotes and
T3109 535-539 CD denotes 12.5
T3110 540-542 NN denotes μg
T3111 552-555 NN denotes RNA
T3112 542-543 SYM denotes /
T3113 543-545 NN denotes ml
T3114 546-551 NN denotes yeast
T3115 555-556 -RRB- denotes )
T3116 556-557 . denotes .
T3117 557-685 sentence denotes For nuclease P1 footprinting assay, binding reactions were carried out under the same conditions except that CaCl2 was omitted.
T3118 558-561 IN denotes For
T3119 617-624 VBN denotes carried
T3120 562-570 NN denotes nuclease
T3121 574-586 NN denotes footprinting
T3122 571-573 NN denotes P1
T3123 587-592 NN denotes assay
T3124 592-594 , denotes ,
T3125 594-601 NN denotes binding
T3126 602-611 NNS denotes reactions
T3127 612-616 VBD denotes were
T3128 625-628 RP denotes out
T3129 629-634 IN denotes under
T3130 635-638 DT denotes the
T3131 644-654 NNS denotes conditions
T3132 639-643 JJ denotes same
T3133 655-661 IN denotes except
T3134 662-666 IN denotes that
T3135 677-684 VBN denotes omitted
T3136 667-672 NN denotes CaCl2
T3137 673-676 VBD denotes was
T3138 684-685 . denotes .
T3139 685-770 sentence denotes P1 nuclease (0.3 U, Roche) was added and incubation was continued at 37°C for 1 min.
T3140 686-688 NN denotes P1
T3141 689-697 NN denotes nuclease
T3142 717-722 VBN denotes added
T3143 698-699 -LRB- denotes (
T3144 706-711 NNP denotes Roche
T3145 699-702 CD denotes 0.3
T3146 703-704 NNP denotes U
T3147 704-706 , denotes ,
T3148 711-712 -RRB- denotes )
T3149 713-716 VBD denotes was
T3150 723-726 CC denotes and
T3151 727-737 NN denotes incubation
T3152 742-751 VBN denotes continued
T3153 738-741 VBD denotes was
T3154 752-754 IN denotes at
T3155 755-757 CD denotes 37
T3156 757-759 NN denotes °C
T3157 760-763 IN denotes for
T3158 764-765 CD denotes 1
T3159 766-769 NN denotes min
T3160 769-770 . denotes .
T3161 770-965 sentence denotes Cleavage was stopped by addition of 50 μl of 25 mM EDTA and 0.4 M NaOH; the mixture was incubated at room temperature for 10 min followed by the addition of 12 μl of 3 M sodium acetate (pH 4.8).
T3162 771-779 NN denotes Cleavage
T3163 784-791 VBN denotes stopped
T3164 780-783 VBD denotes was
T3165 859-868 VBN denotes incubated
T3166 792-794 IN denotes by
T3167 795-803 NN denotes addition
T3168 804-806 IN denotes of
T3169 807-809 CD denotes 50
T3170 810-812 NN denotes μl
T3171 813-815 IN denotes of
T3172 816-818 CD denotes 25
T3173 819-821 NN denotes mM
T3174 822-826 NN denotes EDTA
T3175 827-830 CC denotes and
T3176 831-834 CD denotes 0.4
T3177 835-836 NN denotes M
T3178 837-841 NN denotes NaOH
T3179 841-842 : denotes ;
T3180 843-846 DT denotes the
T3181 847-854 NN denotes mixture
T3182 855-858 VBD denotes was
T3183 869-871 IN denotes at
T3184 872-876 NN denotes room
T3185 877-888 NN denotes temperature
T3186 889-892 IN denotes for
T3187 893-895 CD denotes 10
T3188 896-899 NN denotes min
T3189 900-908 VBN denotes followed
T3190 909-911 IN denotes by
T3191 912-915 DT denotes the
T3192 916-924 NN denotes addition
T3193 925-927 IN denotes of
T3194 928-930 CD denotes 12
T3195 931-933 NN denotes μl
T3196 934-936 IN denotes of
T3197 937-938 CD denotes 3
T3198 939-940 NN denotes M
T3199 948-955 NN denotes acetate
T3200 941-947 NN denotes sodium
T3201 956-957 -LRB- denotes (
T3202 957-959 NN denotes pH
T3203 960-963 CD denotes 4.8
T3204 963-964 -RRB- denotes )
T3205 964-965 . denotes .
T3206 965-1104 sentence denotes Proteins were removed by phenol–chloroform extraction, and DNAs were collected by ethanol precipitation followed by wash with 70% ethanol.
T3207 966-974 NNS denotes Proteins
T3208 980-987 VBN denotes removed
T3209 975-979 VBD denotes were
T3210 988-990 IN denotes by
T3211 991-997 NN denotes phenol
T3212 998-1008 NN denotes chloroform
T3213 997-998 HYPH denotes –
T3214 1009-1019 NN denotes extraction
T3215 1019-1021 , denotes ,
T3216 1021-1024 CC denotes and
T3217 1025-1029 NNS denotes DNAs
T3218 1035-1044 VBN denotes collected
T3219 1030-1034 VBD denotes were
T3220 1045-1047 IN denotes by
T3221 1048-1055 NN denotes ethanol
T3222 1056-1069 NN denotes precipitation
T3223 1070-1078 VBN denotes followed
T3224 1079-1081 IN denotes by
T3225 1082-1086 NN denotes wash
T3226 1087-1091 IN denotes with
T3227 1092-1094 CD denotes 70
T3228 1094-1095 NN denotes %
T3229 1096-1103 NN denotes ethanol
T3230 1103-1104 . denotes .
T3231 1104-1254 sentence denotes The digested DNA was subjected to electrophoresis through a 10 or 12% polyacrylamide sequencing gel containing 7 M urea, followed by autoradiography.
T3232 1105-1108 DT denotes The
T3233 1118-1121 NN denotes DNA
T3234 1109-1117 VBN denotes digested
T3235 1126-1135 VBN denotes subjected
T3236 1122-1125 VBD denotes was
T3237 1136-1138 IN denotes to
T3238 1139-1154 NN denotes electrophoresis
T3239 1155-1162 IN denotes through
T3240 1163-1164 DT denotes a
T3241 1201-1204 NN denotes gel
T3242 1165-1167 CD denotes 10
T3243 1173-1174 NN denotes %
T3244 1168-1170 CC denotes or
T3245 1171-1173 CD denotes 12
T3246 1175-1189 NN denotes polyacrylamide
T3247 1190-1200 NN denotes sequencing
T3248 1205-1215 VBG denotes containing
T3249 1216-1217 CD denotes 7
T3250 1218-1219 NN denotes M
T3251 1220-1224 NN denotes urea
T3252 1224-1226 , denotes ,
T3253 1226-1234 VBN denotes followed
T3254 1235-1237 IN denotes by
T3255 1238-1253 NN denotes autoradiography
T3256 1253-1254 . denotes .
R1882 T2971 T2972 det The,mixtures
R1883 T2972 T2974 nsubj mixtures,contained
R1884 T2973 T2972 compound reaction,mixtures
R1885 T2975 T2976 punct (,μl
R1886 T2976 T2972 parataxis μl,mixtures
R1887 T2977 T2976 nummod 25,μl
R1888 T2978 T2976 punct ),μl
R1889 T2979 T2980 nummod 25,mM
R1890 T2980 T2981 compound mM,NaOH
R1891 T2981 T2974 dobj NaOH,contained
R1892 T2982 T2981 compound HEPES,NaOH
R1893 T2983 T2981 punct –,NaOH
R1894 T2984 T2985 punct (,pH
R1895 T2985 T2981 parataxis pH,NaOH
R1896 T2986 T2985 nummod 7.5,pH
R1897 T2987 T2985 punct ),pH
R1898 T2988 T2981 punct ", ",NaOH
R1899 T2989 T2990 nummod 50,mM
R1900 T2990 T2991 compound mM,acetate
R1901 T2991 T2981 conj acetate,NaOH
R1902 T2992 T2991 compound sodium,acetate
R1903 T2993 T2991 punct ", ",acetate
R1904 T2994 T2995 nummod 10,mM
R1905 T2995 T2996 nmod mM,CH3COO
R1906 T2996 T2991 conj CH3COO,acetate
R1907 T2997 T2996 nmod Mg,CH3COO
R1908 T2998 T2996 punct (,CH3COO
R1909 T2999 T2996 punct ),CH3COO
R1910 T3000 T2996 nummod 2,CH3COO
R1911 T3001 T2996 punct ", ",CH3COO
R1912 T3002 T3003 nummod 1,mM
R1913 T3003 T3004 compound mM,DTT
R1914 T3004 T2996 conj DTT,CH3COO
R1915 T3005 T3004 punct ", ",DTT
R1916 T3006 T3007 nummod 0.25,mg
R1917 T3007 T3008 nmod mg,BSA
R1918 T3008 T3004 conj BSA,DTT
R1919 T3009 T3010 punct /,ml
R1920 T3010 T3007 prep ml,mg
R1921 T3011 T3008 punct ", ",BSA
R1922 T3012 T3013 nummod 0.5,mM
R1923 T3013 T3014 nmod mM,S
R1924 T3014 T3008 conj S,BSA
R1925 T3015 T3014 nmod ATP,S
R1926 T3016 T3014 punct -,S
R1927 T3017 T3014 punct γ,S
R1928 T3018 T3014 punct -,S
R1929 T3019 T3014 punct ", ",S
R1930 T3020 T3021 nummod 0.5,mM
R1931 T3021 T3022 compound mM,CaCl2
R1932 T3022 T3014 conj CaCl2,S
R1933 T3023 T3022 punct ", ",CaCl2
R1934 T3024 T3025 nummod 10,fmol
R1935 T3025 T3022 conj fmol,CaCl2
R1936 T3026 T3025 prep of,fmol
R1937 T3027 T3028 npadvmod 32P,labeled
R1938 T3028 T3030 amod labeled,substrate
R1939 T3029 T3028 punct -,labeled
R1940 T3030 T3026 pobj substrate,of
R1941 T3031 T3030 compound bubble,substrate
R1942 T3032 T3025 punct ", ",fmol
R1943 T3033 T3025 cc and,fmol
R1944 T3034 T3035 det the,amount
R1945 T3035 T3025 conj amount,fmol
R1946 T3036 T3035 amod indicated,amount
R1947 T3037 T3035 prep of,amount
R1948 T3038 T3039 nmod Mcm4,proteins
R1949 T3039 T3037 pobj proteins,of
R1950 T3040 T3038 punct /,Mcm4
R1951 T3041 T3038 nummod 6,Mcm4
R1952 T3042 T3038 punct /,Mcm4
R1953 T3043 T3038 nummod 7,Mcm4
R1954 T3044 T2974 punct .,contained
R1955 T3046 T3047 prep After,added
R1956 T3048 T3046 pobj incubation,After
R1957 T3049 T3048 prep at,incubation
R1958 T3050 T3051 nummod 30,°C
R1959 T3051 T3049 pobj °C,at
R1960 T3052 T3048 prep for,incubation
R1961 T3053 T3054 nummod 30,min
R1962 T3054 T3052 pobj min,for
R1963 T3055 T3047 punct ", ",added
R1964 T3056 T3057 det the,amount
R1965 T3057 T3047 nsubjpass amount,added
R1966 T3058 T3057 amod indicated,amount
R1967 T3059 T3057 prep of,amount
R1968 T3060 T3059 pobj DNase,of
R1969 T3061 T3060 nummod I,DNase
R1970 T3062 T3063 punct (,Biomedical
R1971 T3063 T3060 parataxis Biomedical,DNase
R1972 T3064 T3063 compound TAKARA,Biomedical
R1973 T3065 T3063 punct ", ",Biomedical
R1974 T3066 T3063 npadvmod Japan,Biomedical
R1975 T3067 T3063 punct ),Biomedical
R1976 T3068 T3047 auxpass was,added
R1977 T3069 T3047 punct ", ",added
R1978 T3070 T3047 cc and,added
R1979 T3071 T3072 det the,mixtures
R1980 T3072 T3073 nsubjpass mixtures,incubated
R1981 T3073 T3047 conj incubated,added
R1982 T3074 T3073 auxpass were,incubated
R1983 T3075 T3073 prep at,incubated
R1984 T3076 T3077 compound room,temperature
R1985 T3077 T3075 pobj temperature,at
R1986 T3078 T3073 prep for,incubated
R1987 T3079 T3080 nummod 1,min
R1988 T3080 T3078 pobj min,for
R1989 T3081 T3047 punct .,added
R1990 T3083 T3084 nsubjpass DNase,inactivated
R1991 T3085 T3083 nummod I,DNase
R1992 T3086 T3084 auxpass was,inactivated
R1993 T3087 T3084 agent by,inactivated
R1994 T3088 T3089 det the,addition
R1995 T3089 T3087 pobj addition,by
R1996 T3090 T3089 prep of,addition
R1997 T3091 T3092 nummod 250,μl
R1998 T3092 T3090 pobj μl,of
R1999 T3093 T3092 prep of,μl
R2000 T3094 T3095 compound stop,solution
R2001 T3095 T3093 pobj solution,of
R2002 T3096 T3097 punct (,EDTA
R2003 T3097 T3084 parataxis EDTA,inactivated
R2004 T3098 T3099 nummod 20,mM
R2005 T3099 T3097 compound mM,EDTA
R2006 T3100 T3097 punct ", ",EDTA
R2007 T3101 T3102 compound 0.2M,chloride
R2008 T3102 T3097 conj chloride,EDTA
R2009 T3103 T3102 compound sodium,chloride
R2010 T3104 T3102 punct ", ",chloride
R2011 T3105 T3106 nummod 1,%
R2012 T3106 T3107 compound %,SDS
R2013 T3107 T3102 conj SDS,chloride
R2014 T3108 T3107 cc and,SDS
R2015 T3109 T3110 nummod 12.5,μg
R2016 T3110 T3111 nmod μg,RNA
R2017 T3111 T3107 conj RNA,SDS
R2018 T3112 T3113 punct /,ml
R2019 T3113 T3110 prep ml,μg
R2020 T3114 T3111 compound yeast,RNA
R2021 T3115 T3111 punct ),RNA
R2022 T3116 T3084 punct .,inactivated
R2023 T3118 T3119 prep For,carried
R2024 T3120 T3121 compound nuclease,footprinting
R2025 T3121 T3123 compound footprinting,assay
R2026 T3122 T3121 compound P1,footprinting
R2027 T3123 T3118 pobj assay,For
R2028 T3124 T3119 punct ", ",carried
R2029 T3125 T3126 compound binding,reactions
R2030 T3126 T3119 nsubjpass reactions,carried
R2031 T3127 T3119 auxpass were,carried
R2032 T3128 T3119 prt out,carried
R2033 T3129 T3119 prep under,carried
R2034 T3130 T3131 det the,conditions
R2035 T3131 T3129 pobj conditions,under
R2036 T3132 T3131 amod same,conditions
R2037 T3133 T3131 prep except,conditions
R2038 T3134 T3135 mark that,omitted
R2039 T3135 T3133 pcomp omitted,except
R2040 T3136 T3135 nsubjpass CaCl2,omitted
R2041 T3137 T3135 auxpass was,omitted
R2042 T3138 T3119 punct .,carried
R2043 T3140 T3141 compound P1,nuclease
R2044 T3141 T3142 nsubjpass nuclease,added
R2045 T3143 T3144 punct (,Roche
R2046 T3144 T3141 parataxis Roche,nuclease
R2047 T3145 T3146 nummod 0.3,U
R2048 T3146 T3144 dep U,Roche
R2049 T3147 T3144 punct ", ",Roche
R2050 T3148 T3144 punct ),Roche
R2051 T3149 T3142 auxpass was,added
R2052 T3150 T3142 cc and,added
R2053 T3151 T3152 nsubjpass incubation,continued
R2054 T3152 T3142 conj continued,added
R2055 T3153 T3152 auxpass was,continued
R2056 T3154 T3152 prep at,continued
R2057 T3155 T3156 nummod 37,°C
R2058 T3156 T3154 pobj °C,at
R2059 T3157 T3152 prep for,continued
R2060 T3158 T3159 nummod 1,min
R2061 T3159 T3157 pobj min,for
R2062 T3160 T3152 punct .,continued
R2063 T3162 T3163 nsubjpass Cleavage,stopped
R2064 T3163 T3165 ccomp stopped,incubated
R2065 T3164 T3163 auxpass was,stopped
R2066 T3166 T3163 prep by,stopped
R2067 T3167 T3166 pobj addition,by
R2068 T3168 T3167 prep of,addition
R2069 T3169 T3170 nummod 50,μl
R2070 T3170 T3168 pobj μl,of
R2071 T3171 T3170 prep of,μl
R2072 T3172 T3173 nummod 25,mM
R2073 T3173 T3174 compound mM,EDTA
R2074 T3174 T3171 pobj EDTA,of
R2075 T3175 T3174 cc and,EDTA
R2076 T3176 T3177 nummod 0.4,M
R2077 T3177 T3178 compound M,NaOH
R2078 T3178 T3174 conj NaOH,EDTA
R2079 T3179 T3165 punct ;,incubated
R2080 T3180 T3181 det the,mixture
R2081 T3181 T3165 nsubjpass mixture,incubated
R2082 T3182 T3165 auxpass was,incubated
R2083 T3183 T3165 prep at,incubated
R2084 T3184 T3185 compound room,temperature
R2085 T3185 T3183 pobj temperature,at
R2086 T3186 T3165 prep for,incubated
R2087 T3187 T3188 nummod 10,min
R2088 T3188 T3186 pobj min,for
R2089 T3189 T3188 acl followed,min
R2090 T3190 T3189 agent by,followed
R2091 T3191 T3192 det the,addition
R2092 T3192 T3190 pobj addition,by
R2093 T3193 T3192 prep of,addition
R2094 T3194 T3195 nummod 12,μl
R2095 T3195 T3193 pobj μl,of
R2096 T3196 T3195 prep of,μl
R2097 T3197 T3198 nummod 3,M
R2098 T3198 T3199 compound M,acetate
R2099 T3199 T3196 pobj acetate,of
R2100 T3200 T3199 compound sodium,acetate
R2101 T3201 T3202 punct (,pH
R2102 T3202 T3199 parataxis pH,acetate
R2103 T3203 T3202 nummod 4.8,pH
R2104 T3204 T3202 punct ),pH
R2105 T3205 T3165 punct .,incubated
R2106 T3207 T3208 nsubjpass Proteins,removed
R2107 T3209 T3208 auxpass were,removed
R2108 T3210 T3208 prep by,removed
R2109 T3211 T3212 compound phenol,chloroform
R2110 T3212 T3214 compound chloroform,extraction
R2111 T3213 T3212 punct –,chloroform
R2112 T3214 T3210 pobj extraction,by
R2113 T3215 T3208 punct ", ",removed
R2114 T3216 T3208 cc and,removed
R2115 T3217 T3218 nsubjpass DNAs,collected
R2116 T3218 T3208 conj collected,removed
R2117 T3219 T3218 auxpass were,collected
R2118 T3220 T3218 prep by,collected
R2119 T3221 T3222 compound ethanol,precipitation
R2120 T3222 T3220 pobj precipitation,by
R2121 T3223 T3222 acl followed,precipitation
R2122 T3224 T3223 agent by,followed
R2123 T3225 T3224 pobj wash,by
R2124 T3226 T3225 prep with,wash
R2125 T3227 T3228 nummod 70,%
R2126 T3228 T3229 compound %,ethanol
R2127 T3229 T3226 pobj ethanol,with
R2128 T3230 T3218 punct .,collected
R2129 T3232 T3233 det The,DNA
R2130 T3233 T3235 nsubjpass DNA,subjected
R2131 T3234 T3233 amod digested,DNA
R2132 T3236 T3235 auxpass was,subjected
R2133 T3237 T3235 prep to,subjected
R2134 T3238 T3237 pobj electrophoresis,to
R2135 T3239 T3238 prep through,electrophoresis
R2136 T3240 T3241 det a,gel
R2137 T3241 T3239 pobj gel,through
R2138 T3242 T3243 nummod 10,%
R2139 T3243 T3241 compound %,gel
R2140 T3244 T3242 cc or,10
R2141 T3245 T3242 conj 12,10
R2142 T3246 T3241 compound polyacrylamide,gel
R2143 T3247 T3241 compound sequencing,gel
R2144 T3248 T3241 acl containing,gel
R2145 T3249 T3250 nummod 7,M
R2146 T3250 T3251 compound M,urea
R2147 T3251 T3248 dobj urea,containing
R2148 T3252 T3238 punct ", ",electrophoresis
R2149 T3253 T3238 acl followed,electrophoresis
R2150 T3254 T3253 agent by,followed
R2151 T3255 T3254 pobj autoradiography,by
R2152 T3256 T3235 punct .,subjected

craft-ca-core-ex-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T2927 13-21 CHEBI:60004 denotes mixtures
T2928 46-51 CHEBI:46756 denotes HEPES
T2929 52-56 CHEBI:32145 denotes NaOH
T2930 73-87 CHEBI:32954 denotes sodium acetate
T2931 95-106 CHEBI:62964 denotes Mg(CH3COO)2
T2932 113-116 CHEBI:18320 denotes DTT
T2933 141-148 CHEBI:27575 denotes ATP-γ-S
T2934 157-162 CHEBI:3312 denotes CaCl2
T2935 175-178 CHEBI:37972 denotes 32P
T2936 179-186 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes labeled
T2937 233-237 PR_EXT:000010246 denotes Mcm4
T2938 233-241 GO:0097373 denotes Mcm4/6/7
T2939 242-250 CHEBI_PR_EXT:protein denotes proteins
T2940 313-320 GO_PR_EXT:deoxyribonuclease_I denotes DNase I
T2941 367-375 CHEBI:60004 denotes mixtures
T2942 422-429 GO_PR_EXT:deoxyribonuclease_I denotes DNase I
T2943 480-488 CHEBI:75958 denotes solution
T2944 496-500 CHEBI_EXT:EDTA denotes EDTA
T2945 507-522 CHEBI:26710 denotes sodium chloride
T2946 527-530 CHEBI:8984 denotes SDS
T2947 546-551 NCBITaxon_EXT:yeast denotes yeast
T2948 552-555 CHEBI_SO_EXT:RNA denotes RNA
T2949 562-570 GO_EXT:0004518 denotes nuclease
T2950 594-601 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes binding
T2951 667-672 CHEBI:3312 denotes CaCl2
T2952 689-697 GO_EXT:0004518 denotes nuclease
T2953 771-779 MOP:0000780 denotes Cleavage
T2954 822-826 CHEBI_EXT:EDTA denotes EDTA
T2955 837-841 CHEBI:32145 denotes NaOH
T2956 847-854 CHEBI:60004 denotes mixture
T2957 941-955 CHEBI:32954 denotes sodium acetate
T2958 966-974 CHEBI_PR_EXT:protein denotes Proteins
T2959 991-997 CHEBI:15882 denotes phenol
T2960 998-1008 CHEBI:35255 denotes chloroform
T2961 1025-1029 CHEBI_SO_EXT:DNA denotes DNAs
T2962 1048-1055 CHEBI:16236 denotes ethanol
T2963 1096-1103 CHEBI:16236 denotes ethanol
T2964 1118-1121 CHEBI_SO_EXT:DNA denotes DNA
T2965 1175-1189 CHEBI_EXT:polyacrylamide denotes polyacrylamide
T2966 1220-1224 CHEBI:16199 denotes urea

craft-ca-core-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T2901 13-21 CHEBI:60004 denotes mixtures
T2902 46-51 CHEBI:46756 denotes HEPES
T2903 52-56 CHEBI:32145 denotes NaOH
T2904 73-87 CHEBI:32954 denotes sodium acetate
T2905 95-106 CHEBI:62964 denotes Mg(CH3COO)2
T2906 113-116 CHEBI:18320 denotes DTT
T2907 141-148 CHEBI:27575 denotes ATP-γ-S
T2908 157-162 CHEBI:3312 denotes CaCl2
T2909 175-178 CHEBI:37972 denotes 32P
T2910 233-237 PR:000010246 denotes Mcm4
T2911 233-241 GO:0097373 denotes Mcm4/6/7
T2912 367-375 CHEBI:60004 denotes mixtures
T2913 480-488 CHEBI:75958 denotes solution
T2914 507-522 CHEBI:26710 denotes sodium chloride
T2915 527-530 CHEBI:8984 denotes SDS
T2916 667-672 CHEBI:3312 denotes CaCl2
T2917 771-779 MOP:0000780 denotes Cleavage
T2918 837-841 CHEBI:32145 denotes NaOH
T2919 847-854 CHEBI:60004 denotes mixture
T2920 941-955 CHEBI:32954 denotes sodium acetate
T2921 991-997 CHEBI:15882 denotes phenol
T2922 998-1008 CHEBI:35255 denotes chloroform
T2923 1048-1055 CHEBI:16236 denotes ethanol
T2924 1096-1103 CHEBI:16236 denotes ethanol
T2925 1220-1224 CHEBI:16199 denotes urea