PMC:1140370 / 8769-9023 JSONTXT

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    craft-sa-dev

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containing 25 mM HEPES–NaOH (pH 7.5), 50 mM sodium acetate, 10 mM Mg(CH3COO)2, 1 mM DTT, 0.25 μg/ml BSA, 0.5 mM ATP-γ-S and labeled substrates of the amount indicated. After addition of 2 μl of 50% glycerol, the reaction mixtures were directly applied "}

    craft-ca-core-ex-dev

    {"project":"craft-ca-core-ex-dev","denotations":[{"id":"T2608","span":{"begin":19,"end":24},"obj":"CHEBI:46756"},{"id":"T2609","span":{"begin":25,"end":29},"obj":"CHEBI:32145"},{"id":"T2610","span":{"begin":46,"end":60},"obj":"CHEBI:32954"},{"id":"T2611","span":{"begin":68,"end":79},"obj":"CHEBI:62964"},{"id":"T2612","span":{"begin":86,"end":89},"obj":"CHEBI:18320"},{"id":"T2613","span":{"begin":114,"end":121},"obj":"CHEBI:27575"},{"id":"T2614","span":{"begin":126,"end":133},"obj":"CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process"},{"id":"T2615","span":{"begin":200,"end":208},"obj":"CHEBI:17754"},{"id":"T2616","span":{"begin":223,"end":231},"obj":"CHEBI:60004"}],"text":") containing 25 mM HEPES–NaOH (pH 7.5), 50 mM sodium acetate, 10 mM Mg(CH3COO)2, 1 mM DTT, 0.25 μg/ml BSA, 0.5 mM ATP-γ-S and labeled substrates of the amount indicated. After addition of 2 μl of 50% glycerol, the reaction mixtures were directly applied "}

    craft-ca-core-dev

    {"project":"craft-ca-core-dev","denotations":[{"id":"T2590","span":{"begin":19,"end":24},"obj":"CHEBI:46756"},{"id":"T2591","span":{"begin":25,"end":29},"obj":"CHEBI:32145"},{"id":"T2592","span":{"begin":46,"end":60},"obj":"CHEBI:32954"},{"id":"T2593","span":{"begin":68,"end":79},"obj":"CHEBI:62964"},{"id":"T2594","span":{"begin":86,"end":89},"obj":"CHEBI:18320"},{"id":"T2595","span":{"begin":114,"end":121},"obj":"CHEBI:27575"},{"id":"T2596","span":{"begin":200,"end":208},"obj":"CHEBI:17754"},{"id":"T2597","span":{"begin":223,"end":231},"obj":"CHEBI:60004"}],"text":") containing 25 mM HEPES–NaOH (pH 7.5), 50 mM sodium acetate, 10 mM Mg(CH3COO)2, 1 mM DTT, 0.25 μg/ml BSA, 0.5 mM ATP-γ-S and labeled substrates of the amount indicated. After addition of 2 μl of 50% glycerol, the reaction mixtures were directly applied "}