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PMC:1140370 / 8669-9610 JSONTXT

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craft-sa-dev

Id Subject Object Predicate Lexical cue
T2636 0-2 IN denotes In
T2637 44-53 VBN denotes incubated
T2638 3-6 NN denotes gel
T2639 7-12 NN denotes shift
T2640 13-19 NNS denotes assays
T2641 19-21 , denotes ,
T2642 21-25 NN denotes Mcm4
T2643 30-38 NN denotes proteins
T2644 25-26 HYPH denotes /
T2645 26-27 CD denotes 6
T2646 27-28 HYPH denotes /
T2647 28-29 CD denotes 7
T2648 39-43 VBD denotes were
T2649 54-56 IN denotes at
T2650 57-59 CD denotes 30
T2651 59-61 NN denotes °C
T2652 62-65 IN denotes for
T2653 66-68 CD denotes 30
T2654 69-72 NN denotes min
T2655 73-75 IN denotes in
T2656 76-84 NN denotes reaction
T2657 85-93 NNS denotes mixtures
T2658 94-95 -LRB- denotes (
T2659 98-100 NN denotes μl
T2660 95-97 CD denotes 15
T2661 100-101 -RRB- denotes )
T2662 102-112 VBG denotes containing
T2663 113-115 CD denotes 25
T2664 116-118 NN denotes mM
T2665 125-129 NN denotes NaOH
T2666 119-124 NN denotes HEPES
T2667 124-125 HYPH denotes –
T2668 130-131 -LRB- denotes (
T2669 131-133 NN denotes pH
T2670 134-137 CD denotes 7.5
T2671 137-138 -RRB- denotes )
T2672 138-140 , denotes ,
T2673 140-142 CD denotes 50
T2674 143-145 NN denotes mM
T2675 153-160 NN denotes acetate
T2676 146-152 NN denotes sodium
T2677 160-162 , denotes ,
T2678 162-164 CD denotes 10
T2679 165-167 NN denotes mM
T2680 171-177 NN denotes CH3COO
T2681 168-170 NN denotes Mg
T2682 170-171 -LRB- denotes (
T2683 177-178 -RRB- denotes )
T2684 178-179 CD denotes 2
T2685 179-181 , denotes ,
T2686 181-182 CD denotes 1
T2687 183-185 NN denotes mM
T2688 186-189 NN denotes DTT
T2689 189-191 , denotes ,
T2690 191-195 CD denotes 0.25
T2691 196-198 NN denotes μg
T2692 202-205 NN denotes BSA
T2693 198-199 SYM denotes /
T2694 199-201 NN denotes ml
T2695 205-207 , denotes ,
T2696 207-210 CD denotes 0.5
T2697 211-213 NN denotes mM
T2698 220-221 NN denotes S
T2699 214-217 NN denotes ATP
T2700 217-218 HYPH denotes -
T2701 218-219 SYM denotes γ
T2702 219-220 HYPH denotes -
T2703 222-225 CC denotes and
T2704 226-233 VBN denotes labeled
T2705 234-244 NNS denotes substrates
T2706 245-247 IN denotes of
T2707 248-251 DT denotes the
T2708 252-258 NN denotes amount
T2709 259-268 VBN denotes indicated
T2710 268-269 . denotes .
T2711 269-495 sentence denotes After addition of 2 μl of 50% glycerol, the reaction mixtures were directly applied on a polyacrylamide gel containing 6 mM magnesium acetate and 5% glycerol in 0.5× TBE, and electrophoresis was conducted at room temperature.
T2712 270-275 IN denotes After
T2713 346-353 VBN denotes applied
T2714 276-284 NN denotes addition
T2715 285-287 IN denotes of
T2716 288-289 CD denotes 2
T2717 290-292 NN denotes μl
T2718 293-295 IN denotes of
T2719 296-298 CD denotes 50
T2720 298-299 NN denotes %
T2721 300-308 NN denotes glycerol
T2722 308-310 , denotes ,
T2723 310-313 DT denotes the
T2724 323-331 NNS denotes mixtures
T2725 314-322 NN denotes reaction
T2726 332-336 VBD denotes were
T2727 337-345 RB denotes directly
T2728 354-356 IN denotes on
T2729 357-358 DT denotes a
T2730 374-377 NN denotes gel
T2731 359-373 NN denotes polyacrylamide
T2732 378-388 VBG denotes containing
T2733 389-390 CD denotes 6
T2734 391-393 NN denotes mM
T2735 404-411 NN denotes acetate
T2736 394-403 NN denotes magnesium
T2737 412-415 CC denotes and
T2738 416-417 CD denotes 5
T2739 417-418 NN denotes %
T2740 419-427 NN denotes glycerol
T2741 428-430 IN denotes in
T2742 431-434 CD denotes 0.5
T2743 436-439 NN denotes TBE
T2744 434-435 SYM denotes ×
T2745 439-441 , denotes ,
T2746 441-444 CC denotes and
T2747 445-460 NN denotes electrophoresis
T2748 465-474 VBN denotes conducted
T2749 461-464 VBD denotes was
T2750 475-477 IN denotes at
T2751 478-482 NN denotes room
T2752 483-494 NN denotes temperature
T2753 494-495 . denotes .
T2754 495-713 sentence denotes For DNA helicase assays, the reaction mixtures of the gel shift assays, as described above, were incubated at 30°C for 15 min, and then the ATP was added at 10 mM, followed by incubation at 37°C for additional 30 min.
T2755 496-499 IN denotes For
T2756 593-602 VBN denotes incubated
T2757 500-503 NN denotes DNA
T2758 513-519 NNS denotes assays
T2759 504-512 NN denotes helicase
T2760 519-521 , denotes ,
T2761 521-524 DT denotes the
T2762 534-542 NNS denotes mixtures
T2763 525-533 NN denotes reaction
T2764 543-545 IN denotes of
T2765 546-549 DT denotes the
T2766 560-566 NNS denotes assays
T2767 550-553 NN denotes gel
T2768 554-559 NN denotes shift
T2769 566-568 , denotes ,
T2770 568-570 IN denotes as
T2771 571-580 VBN denotes described
T2772 581-586 RB denotes above
T2773 586-588 , denotes ,
T2774 588-592 VBD denotes were
T2775 603-605 IN denotes at
T2776 606-608 CD denotes 30
T2777 608-610 NN denotes °C
T2778 611-614 IN denotes for
T2779 615-617 CD denotes 15
T2780 618-621 NN denotes min
T2781 621-623 , denotes ,
T2782 623-626 CC denotes and
T2783 627-631 RB denotes then
T2784 632-635 DT denotes the
T2785 636-639 NN denotes ATP
T2786 644-649 VBN denotes added
T2787 640-643 VBD denotes was
T2788 650-652 IN denotes at
T2789 653-655 CD denotes 10
T2790 656-658 NN denotes mM
T2791 658-660 , denotes ,
T2792 660-668 VBN denotes followed
T2793 669-671 IN denotes by
T2794 672-682 NN denotes incubation
T2795 683-685 IN denotes at
T2796 686-688 CD denotes 37
T2797 688-690 NN denotes °C
T2798 691-694 IN denotes for
T2799 695-705 JJ denotes additional
T2800 709-712 NN denotes min
T2801 706-708 CD denotes 30
T2802 712-713 . denotes .
T2803 713-848 sentence denotes The reactions were terminated by addition of EDTA (20 mM), SDS (0.1%) and 2 μg proteinase K, and were incubated for additional 15 min.
T2804 714-717 DT denotes The
T2805 718-727 NNS denotes reactions
T2806 733-743 VBN denotes terminated
T2807 728-732 VBD denotes were
T2808 744-746 IN denotes by
T2809 747-755 NN denotes addition
T2810 756-758 IN denotes of
T2811 759-763 NN denotes EDTA
T2812 764-765 -LRB- denotes (
T2813 768-770 NN denotes mM
T2814 765-767 CD denotes 20
T2815 770-771 -RRB- denotes )
T2816 771-773 , denotes ,
T2817 773-776 NN denotes SDS
T2818 777-778 -LRB- denotes (
T2819 781-782 NN denotes %
T2820 778-781 CD denotes 0.1
T2821 782-783 -RRB- denotes )
T2822 784-787 CC denotes and
T2823 788-789 CD denotes 2
T2824 790-792 NN denotes μg
T2825 804-805 NN denotes K
T2826 793-803 NN denotes proteinase
T2827 805-807 , denotes ,
T2828 807-810 CC denotes and
T2829 811-815 VBD denotes were
T2830 816-825 VBN denotes incubated
T2831 826-829 IN denotes for
T2832 830-840 JJ denotes additional
T2833 844-847 NN denotes min
T2834 841-843 CD denotes 15
T2835 847-848 . denotes .
T2836 848-941 sentence denotes The samples were separated by electrophoresis on nondenaturing polyacrylamide gel in 1× TBE.
T2837 849-852 DT denotes The
T2838 853-860 NNS denotes samples
T2839 866-875 VBN denotes separated
T2840 861-865 VBD denotes were
T2841 876-878 IN denotes by
T2842 879-894 NN denotes electrophoresis
T2843 895-897 IN denotes on
T2844 898-911 JJ denotes nondenaturing
T2845 927-930 NN denotes gel
T2846 912-926 NN denotes polyacrylamide
T2847 931-933 IN denotes in
T2848 934-935 CD denotes 1
T2849 937-940 NN denotes TBE
T2850 935-936 SYM denotes ×
T2851 940-941 . denotes .
R1673 T2636 T2637 prep In,incubated
R1674 T2638 T2639 compound gel,shift
R1675 T2639 T2640 compound shift,assays
R1676 T2640 T2636 pobj assays,In
R1677 T2641 T2637 punct ", ",incubated
R1678 T2642 T2643 nmod Mcm4,proteins
R1679 T2643 T2637 nsubjpass proteins,incubated
R1680 T2644 T2642 punct /,Mcm4
R1681 T2645 T2642 nummod 6,Mcm4
R1682 T2646 T2642 punct /,Mcm4
R1683 T2647 T2642 nummod 7,Mcm4
R1684 T2648 T2637 auxpass were,incubated
R1685 T2649 T2637 prep at,incubated
R1686 T2650 T2651 nummod 30,°C
R1687 T2651 T2649 pobj °C,at
R1688 T2652 T2637 prep for,incubated
R1689 T2653 T2654 nummod 30,min
R1690 T2654 T2652 pobj min,for
R1691 T2655 T2637 prep in,incubated
R1692 T2656 T2657 compound reaction,mixtures
R1693 T2657 T2655 pobj mixtures,in
R1694 T2658 T2659 punct (,μl
R1695 T2659 T2657 parataxis μl,mixtures
R1696 T2660 T2659 nummod 15,μl
R1697 T2661 T2659 punct ),μl
R1698 T2662 T2657 acl containing,mixtures
R1699 T2663 T2664 nummod 25,mM
R1700 T2664 T2665 compound mM,NaOH
R1701 T2665 T2662 dobj NaOH,containing
R1702 T2666 T2665 compound HEPES,NaOH
R1703 T2667 T2665 punct –,NaOH
R1704 T2668 T2669 punct (,pH
R1705 T2669 T2665 parataxis pH,NaOH
R1706 T2670 T2669 nummod 7.5,pH
R1707 T2671 T2669 punct ),pH
R1708 T2672 T2665 punct ", ",NaOH
R1709 T2673 T2674 nummod 50,mM
R1710 T2674 T2675 compound mM,acetate
R1711 T2675 T2665 conj acetate,NaOH
R1712 T2676 T2675 compound sodium,acetate
R1713 T2677 T2675 punct ", ",acetate
R1714 T2678 T2679 nummod 10,mM
R1715 T2679 T2680 nmod mM,CH3COO
R1716 T2680 T2675 conj CH3COO,acetate
R1717 T2681 T2680 nmod Mg,CH3COO
R1718 T2682 T2680 punct (,CH3COO
R1719 T2683 T2680 punct ),CH3COO
R1720 T2684 T2680 nummod 2,CH3COO
R1721 T2685 T2680 punct ", ",CH3COO
R1722 T2686 T2687 nummod 1,mM
R1723 T2687 T2688 compound mM,DTT
R1724 T2688 T2680 conj DTT,CH3COO
R1725 T2689 T2688 punct ", ",DTT
R1726 T2690 T2691 nummod 0.25,μg
R1727 T2691 T2692 nmod μg,BSA
R1728 T2692 T2688 conj BSA,DTT
R1729 T2693 T2694 punct /,ml
R1730 T2694 T2691 prep ml,μg
R1731 T2695 T2692 punct ", ",BSA
R1732 T2696 T2697 nummod 0.5,mM
R1733 T2697 T2698 nmod mM,S
R1734 T2698 T2692 conj S,BSA
R1735 T2699 T2698 nmod ATP,S
R1736 T2700 T2698 punct -,S
R1737 T2701 T2698 punct γ,S
R1738 T2702 T2698 punct -,S
R1739 T2703 T2698 cc and,S
R1740 T2704 T2705 amod labeled,substrates
R1741 T2705 T2698 conj substrates,S
R1742 T2706 T2705 prep of,substrates
R1743 T2707 T2708 det the,amount
R1744 T2708 T2706 pobj amount,of
R1745 T2709 T2708 acl indicated,amount
R1746 T2710 T2637 punct .,incubated
R1747 T2712 T2713 prep After,applied
R1748 T2714 T2712 pobj addition,After
R1749 T2715 T2714 prep of,addition
R1750 T2716 T2717 nummod 2,μl
R1751 T2717 T2715 pobj μl,of
R1752 T2718 T2717 prep of,μl
R1753 T2719 T2720 nummod 50,%
R1754 T2720 T2721 compound %,glycerol
R1755 T2721 T2718 pobj glycerol,of
R1756 T2722 T2713 punct ", ",applied
R1757 T2723 T2724 det the,mixtures
R1758 T2724 T2713 nsubjpass mixtures,applied
R1759 T2725 T2724 compound reaction,mixtures
R1760 T2726 T2713 auxpass were,applied
R1761 T2727 T2713 advmod directly,applied
R1762 T2728 T2713 prep on,applied
R1763 T2729 T2730 det a,gel
R1764 T2730 T2728 pobj gel,on
R1765 T2731 T2730 compound polyacrylamide,gel
R1766 T2732 T2730 acl containing,gel
R1767 T2733 T2734 nummod 6,mM
R1768 T2734 T2735 compound mM,acetate
R1769 T2735 T2732 dobj acetate,containing
R1770 T2736 T2735 compound magnesium,acetate
R1771 T2737 T2735 cc and,acetate
R1772 T2738 T2739 nummod 5,%
R1773 T2739 T2740 compound %,glycerol
R1774 T2740 T2735 conj glycerol,acetate
R1775 T2741 T2732 prep in,containing
R1776 T2742 T2743 nummod 0.5,TBE
R1777 T2743 T2741 pobj TBE,in
R1778 T2744 T2742 punct ×,0.5
R1779 T2745 T2713 punct ", ",applied
R1780 T2746 T2713 cc and,applied
R1781 T2747 T2748 nsubjpass electrophoresis,conducted
R1782 T2748 T2713 conj conducted,applied
R1783 T2749 T2748 auxpass was,conducted
R1784 T2750 T2748 prep at,conducted
R1785 T2751 T2752 compound room,temperature
R1786 T2752 T2750 pobj temperature,at
R1787 T2753 T2748 punct .,conducted
R1788 T2755 T2756 prep For,incubated
R1789 T2757 T2758 compound DNA,assays
R1790 T2758 T2755 pobj assays,For
R1791 T2759 T2758 compound helicase,assays
R1792 T2760 T2756 punct ", ",incubated
R1793 T2761 T2762 det the,mixtures
R1794 T2762 T2756 nsubjpass mixtures,incubated
R1795 T2763 T2762 compound reaction,mixtures
R1796 T2764 T2762 prep of,mixtures
R1797 T2765 T2766 det the,assays
R1798 T2766 T2764 pobj assays,of
R1799 T2767 T2768 compound gel,shift
R1800 T2768 T2766 compound shift,assays
R1801 T2769 T2756 punct ", ",incubated
R1802 T2770 T2771 mark as,described
R1803 T2771 T2756 advcl described,incubated
R1804 T2772 T2771 advmod above,described
R1805 T2773 T2756 punct ", ",incubated
R1806 T2774 T2756 auxpass were,incubated
R1807 T2775 T2756 prep at,incubated
R1808 T2776 T2777 nummod 30,°C
R1809 T2777 T2775 pobj °C,at
R1810 T2778 T2756 prep for,incubated
R1811 T2779 T2780 nummod 15,min
R1812 T2780 T2778 pobj min,for
R1813 T2781 T2756 punct ", ",incubated
R1814 T2782 T2756 cc and,incubated
R1815 T2783 T2782 advmod then,and
R1816 T2784 T2785 det the,ATP
R1817 T2785 T2786 nsubjpass ATP,added
R1818 T2786 T2756 conj added,incubated
R1819 T2787 T2786 auxpass was,added
R1820 T2788 T2786 prep at,added
R1821 T2789 T2790 nummod 10,mM
R1822 T2790 T2788 pobj mM,at
R1823 T2791 T2786 punct ", ",added
R1824 T2792 T2786 advcl followed,added
R1825 T2793 T2792 agent by,followed
R1826 T2794 T2793 pobj incubation,by
R1827 T2795 T2794 prep at,incubation
R1828 T2796 T2797 nummod 37,°C
R1829 T2797 T2795 pobj °C,at
R1830 T2798 T2794 prep for,incubation
R1831 T2799 T2800 amod additional,min
R1832 T2800 T2798 pobj min,for
R1833 T2801 T2800 nummod 30,min
R1834 T2802 T2786 punct .,added
R1835 T2804 T2805 det The,reactions
R1836 T2805 T2806 nsubjpass reactions,terminated
R1837 T2807 T2806 auxpass were,terminated
R1838 T2808 T2806 agent by,terminated
R1839 T2809 T2808 pobj addition,by
R1840 T2810 T2809 prep of,addition
R1841 T2811 T2810 pobj EDTA,of
R1842 T2812 T2813 punct (,mM
R1843 T2813 T2811 parataxis mM,EDTA
R1844 T2814 T2813 nummod 20,mM
R1845 T2815 T2813 punct ),mM
R1846 T2816 T2811 punct ", ",EDTA
R1847 T2817 T2811 conj SDS,EDTA
R1848 T2818 T2819 punct (,%
R1849 T2819 T2817 parataxis %,SDS
R1850 T2820 T2819 nummod 0.1,%
R1851 T2821 T2819 punct ),%
R1852 T2822 T2817 cc and,SDS
R1853 T2823 T2824 nummod 2,μg
R1854 T2824 T2825 compound μg,K
R1855 T2825 T2817 conj K,SDS
R1856 T2826 T2825 compound proteinase,K
R1857 T2827 T2806 punct ", ",terminated
R1858 T2828 T2806 cc and,terminated
R1859 T2829 T2830 auxpass were,incubated
R1860 T2830 T2806 conj incubated,terminated
R1861 T2831 T2830 prep for,incubated
R1862 T2832 T2833 amod additional,min
R1863 T2833 T2831 pobj min,for
R1864 T2834 T2833 nummod 15,min
R1865 T2835 T2806 punct .,terminated
R1866 T2837 T2838 det The,samples
R1867 T2838 T2839 nsubjpass samples,separated
R1868 T2840 T2839 auxpass were,separated
R1869 T2841 T2839 prep by,separated
R1870 T2842 T2841 pobj electrophoresis,by
R1871 T2843 T2839 prep on,separated
R1872 T2844 T2845 amod nondenaturing,gel
R1873 T2845 T2843 pobj gel,on
R1874 T2846 T2845 compound polyacrylamide,gel
R1875 T2847 T2845 prep in,gel
R1876 T2848 T2849 nummod 1,TBE
R1877 T2849 T2847 pobj TBE,in
R1878 T2850 T2848 punct ×,1
R1879 T2851 T2839 punct .,separated

craft-ca-core-ex-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T2604 21-25 PR_EXT:000010246 denotes Mcm4
T2605 21-29 GO:0097373 denotes Mcm4/6/7
T2606 30-38 CHEBI_PR_EXT:protein denotes proteins
T2607 85-93 CHEBI:60004 denotes mixtures
T2608 119-124 CHEBI:46756 denotes HEPES
T2609 125-129 CHEBI:32145 denotes NaOH
T2610 146-160 CHEBI:32954 denotes sodium acetate
T2611 168-179 CHEBI:62964 denotes Mg(CH3COO)2
T2612 186-189 CHEBI:18320 denotes DTT
T2613 214-221 CHEBI:27575 denotes ATP-γ-S
T2614 226-233 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes labeled
T2615 300-308 CHEBI:17754 denotes glycerol
T2616 323-331 CHEBI:60004 denotes mixtures
T2617 359-373 CHEBI_EXT:polyacrylamide denotes polyacrylamide
T2618 394-411 CHEBI:62964 denotes magnesium acetate
T2619 419-427 CHEBI:17754 denotes glycerol
T2620 500-503 CHEBI_SO_EXT:DNA denotes DNA
T2621 500-512 GO_EXT:0003678 denotes DNA helicase
T2622 534-542 CHEBI:60004 denotes mixtures
T2623 636-639 CHEBI_EXT:ATP denotes ATP
T2624 759-763 CHEBI_EXT:EDTA denotes EDTA
T2625 773-776 CHEBI:8984 denotes SDS
T2626 793-803 GO_EXT:0008233 denotes proteinase
T2627 901-911 GO_EXT:macromolecule_denaturation denotes denaturing
T2628 912-926 CHEBI_EXT:polyacrylamide denotes polyacrylamide

craft-ca-core-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T2587 21-25 PR:000010246 denotes Mcm4
T2588 21-29 GO:0097373 denotes Mcm4/6/7
T2589 85-93 CHEBI:60004 denotes mixtures
T2590 119-124 CHEBI:46756 denotes HEPES
T2591 125-129 CHEBI:32145 denotes NaOH
T2592 146-160 CHEBI:32954 denotes sodium acetate
T2593 168-179 CHEBI:62964 denotes Mg(CH3COO)2
T2594 186-189 CHEBI:18320 denotes DTT
T2595 214-221 CHEBI:27575 denotes ATP-γ-S
T2596 300-308 CHEBI:17754 denotes glycerol
T2597 323-331 CHEBI:60004 denotes mixtures
T2598 394-411 CHEBI:62964 denotes magnesium acetate
T2599 419-427 CHEBI:17754 denotes glycerol
T2600 534-542 CHEBI:60004 denotes mixtures
T2601 773-776 CHEBI:8984 denotes SDS