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PMC:1140370 / 7424-8633 JSONTXT

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craft-sa-dev

Id Subject Object Predicate Lexical cue
T2286 0-3 DT denotes The
T2287 25-35 NNS denotes substrates
T2288 4-11 JJ denotes partial
T2289 12-24 NN denotes heteroduplex
T2290 113-121 VBN denotes prepared
T2291 36-38 IN denotes on
T2292 39-40 DT denotes a
T2293 66-69 NN denotes DNA
T2294 41-47 JJ denotes single
T2295 48-56 VBN denotes stranded
T2296 47-48 HYPH denotes -
T2297 57-65 JJ denotes circular
T2298 70-74 IN denotes with
T2299 75-81 VBN denotes varied
T2300 82-89 NNS denotes lengths
T2301 90-92 IN denotes of
T2302 93-99 NN denotes duplex
T2303 100-107 NNS denotes regions
T2304 108-112 VBD denotes were
T2305 122-124 IN denotes by
T2306 125-134 VBG denotes extending
T2307 135-138 NN denotes DNA
T2308 139-145 NNS denotes chains
T2309 146-150 IN denotes from
T2310 151-154 DT denotes the
T2311 158-161 NN denotes end
T2312 155-156 CD denotes 3
T2313 156-157 SYM denotes ′
T2314 162-164 IN denotes of
T2315 165-168 DT denotes the
T2316 174-179 NN denotes 37mer
T2317 169-173 NN denotes dT40
T2318 173-174 HYPH denotes -
T2319 180-181 -LRB- denotes (
T2320 181-186 NN denotes 37mer
T2321 187-193 NN denotes region
T2322 194-205 VBG denotes hybridizing
T2323 206-208 IN denotes at
T2324 209-218 NNS denotes positions
T2325 219-223 CD denotes 6289
T2326 223-224 SYM denotes –
T2327 224-228 CD denotes 6326
T2328 229-231 IN denotes of
T2329 232-239 NN denotes M13mp18
T2330 240-246 NN denotes vector
T2331 246-247 -RRB- denotes )
T2332 248-256 VBN denotes annealed
T2333 257-259 IN denotes to
T2334 260-263 DT denotes the
T2335 289-292 NN denotes DNA
T2336 264-270 JJ denotes single
T2337 271-279 VBN denotes stranded
T2338 270-271 HYPH denotes -
T2339 280-288 JJ denotes circular
T2340 292-293 . denotes .
T2341 293-514 sentence denotes DNA chains were elongated with Sequenase in the presence of [α-32P]dGTP, followed by extension after addition of ddGTP and all four dNTPs, resulting in substrates containing labeled duplex regions of varied lengths (13).
T2342 294-297 NN denotes DNA
T2343 298-304 NNS denotes chains
T2344 310-319 VBN denotes elongated
T2345 305-309 VBD denotes were
T2346 320-324 IN denotes with
T2347 325-334 NN denotes Sequenase
T2348 335-337 IN denotes in
T2349 338-341 DT denotes the
T2350 342-350 NN denotes presence
T2351 351-353 IN denotes of
T2352 354-355 -LRB- denotes [
T2353 361-365 NN denotes dGTP
T2354 355-356 NN denotes α
T2355 357-360 NN denotes 32P
T2356 356-357 HYPH denotes -
T2357 360-361 -RRB- denotes ]
T2358 365-367 , denotes ,
T2359 367-375 VBN denotes followed
T2360 376-378 IN denotes by
T2361 379-388 NN denotes extension
T2362 389-394 IN denotes after
T2363 395-403 NN denotes addition
T2364 404-406 IN denotes of
T2365 407-412 NN denotes ddGTP
T2366 413-416 CC denotes and
T2367 417-420 DT denotes all
T2368 426-431 NNS denotes dNTPs
T2369 421-425 CD denotes four
T2370 431-433 , denotes ,
T2371 433-442 VBG denotes resulting
T2372 443-445 IN denotes in
T2373 446-456 NNS denotes substrates
T2374 457-467 VBG denotes containing
T2375 468-475 VBN denotes labeled
T2376 483-490 NNS denotes regions
T2377 476-482 NN denotes duplex
T2378 491-493 IN denotes of
T2379 494-500 VBN denotes varied
T2380 501-508 NNS denotes lengths
T2381 509-510 -LRB- denotes (
T2382 510-512 CD denotes 13
T2383 512-513 -RRB- denotes )
T2384 513-514 . denotes .
T2385 514-788 sentence denotes The substrate (5 fmol) was first incubated with indicated amount of the Mcm4/6/7 complex at 37°C for 1 h in reaction mixture as described (11), and then reactions were stopped by addition of EDTA (20 mM), SDS (0.1%) and 2 μg proteinase K, followed by incubation for 20 min.
T2386 515-518 DT denotes The
T2387 519-528 NN denotes substrate
T2388 548-557 VBN denotes incubated
T2389 529-530 -LRB- denotes (
T2390 532-536 NN denotes fmol
T2391 530-531 CD denotes 5
T2392 536-537 -RRB- denotes )
T2393 538-541 VBD denotes was
T2394 542-547 RB denotes first
T2395 558-562 IN denotes with
T2396 563-572 VBN denotes indicated
T2397 573-579 NN denotes amount
T2398 580-582 IN denotes of
T2399 583-586 DT denotes the
T2400 596-603 NN denotes complex
T2401 587-591 NN denotes Mcm4
T2402 591-592 HYPH denotes /
T2403 592-593 CD denotes 6
T2404 593-594 HYPH denotes /
T2405 594-595 CD denotes 7
T2406 604-606 IN denotes at
T2407 607-609 CD denotes 37
T2408 609-611 NN denotes °C
T2409 612-615 IN denotes for
T2410 616-617 CD denotes 1
T2411 618-619 NN denotes h
T2412 620-622 IN denotes in
T2413 623-631 NN denotes reaction
T2414 632-639 NN denotes mixture
T2415 640-642 IN denotes as
T2416 643-652 VBN denotes described
T2417 653-654 -LRB- denotes (
T2418 654-656 CD denotes 11
T2419 656-657 -RRB- denotes )
T2420 657-659 , denotes ,
T2421 659-662 CC denotes and
T2422 663-667 RB denotes then
T2423 668-677 NNS denotes reactions
T2424 683-690 VBN denotes stopped
T2425 678-682 VBD denotes were
T2426 691-693 IN denotes by
T2427 694-702 NN denotes addition
T2428 703-705 IN denotes of
T2429 706-710 NN denotes EDTA
T2430 711-712 -LRB- denotes (
T2431 715-717 NN denotes mM
T2432 712-714 CD denotes 20
T2433 717-718 -RRB- denotes )
T2434 718-720 , denotes ,
T2435 720-723 NN denotes SDS
T2436 724-725 -LRB- denotes (
T2437 728-729 NN denotes %
T2438 725-728 CD denotes 0.1
T2439 729-730 -RRB- denotes )
T2440 731-734 CC denotes and
T2441 735-736 CD denotes 2
T2442 737-739 NN denotes μg
T2443 751-752 NN denotes K
T2444 740-750 NN denotes proteinase
T2445 752-754 , denotes ,
T2446 754-762 VBN denotes followed
T2447 763-765 IN denotes by
T2448 766-776 NN denotes incubation
T2449 777-780 IN denotes for
T2450 781-783 CD denotes 20
T2451 784-787 NN denotes min
T2452 787-788 . denotes .
T2453 788-877 sentence denotes Aliquots were electrophoresed on a 6.5% polyacrylamide gel in 1× TBE at 300 V for 2.5 h.
T2454 789-797 NNS denotes Aliquots
T2455 803-818 VBN denotes electrophoresed
T2456 798-802 VBD denotes were
T2457 819-821 IN denotes on
T2458 822-823 DT denotes a
T2459 844-847 NN denotes gel
T2460 824-827 CD denotes 6.5
T2461 827-828 NN denotes %
T2462 829-843 NN denotes polyacrylamide
T2463 848-850 IN denotes in
T2464 851-852 CD denotes 1
T2465 854-857 NN denotes TBE
T2466 852-853 SYM denotes ×
T2467 858-860 IN denotes at
T2468 861-864 CD denotes 300
T2469 865-866 NN denotes V
T2470 867-870 IN denotes for
T2471 871-874 CD denotes 2.5
T2472 875-876 NN denotes h
T2473 876-877 . denotes .
T2474 877-949 sentence denotes The single-stranded circular DNAs used were M13mp18 and M13mp19+G-rich.
T2475 878-881 DT denotes The
T2476 907-911 NNS denotes DNAs
T2477 882-888 JJ denotes single
T2478 889-897 VBN denotes stranded
T2479 888-889 HYPH denotes -
T2480 898-906 JJ denotes circular
T2481 917-921 VBD denotes were
T2482 912-916 VBN denotes used
T2483 922-929 NN denotes M13mp18
T2484 930-933 CC denotes and
T2485 934-941 NN denotes M13mp19
T2486 941-942 SYM denotes +
T2487 942-943 NN denotes G
T2488 943-944 HYPH denotes -
T2489 944-948 JJ denotes rich
T2490 948-949 . denotes .
T2491 949-1209 sentence denotes The latter was constructed by inserting a G-rich repeat sequence (GGGGCGTGGGC)6, prepared by annealing of two oligonucleotides (5′-GATCC-[GGGGCGTGGGC]6-3′/5′-GATCC-[GCCCACGCCCC]6-3′) at the BamHI site of M13mp19 and the sequences of the insert were confirmed.
T2492 950-953 DT denotes The
T2493 954-960 JJ denotes latter
T2494 965-976 VBN denotes constructed
T2495 961-964 VBD denotes was
T2496 977-979 IN denotes by
T2497 980-989 VBG denotes inserting
T2498 990-991 DT denotes a
T2499 1006-1014 NN denotes sequence
T2500 992-993 NN denotes G
T2501 993-994 HYPH denotes -
T2502 994-998 JJ denotes rich
T2503 999-1005 NN denotes repeat
T2504 1015-1016 -LRB- denotes (
T2505 1016-1027 NN denotes GGGGCGTGGGC
T2506 1027-1028 -RRB- denotes )
T2507 1028-1029 CD denotes 6
T2508 1029-1031 , denotes ,
T2509 1031-1039 VBN denotes prepared
T2510 1040-1042 IN denotes by
T2511 1043-1052 VBG denotes annealing
T2512 1053-1055 IN denotes of
T2513 1056-1059 CD denotes two
T2514 1060-1076 NNS denotes oligonucleotides
T2515 1077-1078 -LRB- denotes (
T2516 1078-1079 CD denotes 5
T2517 1079-1080 SYM denotes ′
T2518 1080-1081 HYPH denotes -
T2519 1081-1086 NN denotes GATCC
T2520 1088-1099 NN denotes GGGGCGTGGGC
T2521 1086-1087 HYPH denotes -
T2522 1087-1088 -LRB- denotes [
T2523 1099-1100 -RRB- denotes ]
T2524 1100-1101 CD denotes 6
T2525 1101-1102 HYPH denotes -
T2526 1102-1103 CD denotes 3
T2527 1103-1104 SYM denotes ′
T2528 1104-1105 HYPH denotes /
T2529 1105-1106 CD denotes 5
T2530 1106-1107 SYM denotes ′
T2531 1107-1108 HYPH denotes -
T2532 1108-1113 NN denotes GATCC
T2533 1115-1126 NN denotes GCCCACGCCCC
T2534 1113-1114 HYPH denotes -
T2535 1114-1115 -LRB- denotes [
T2536 1126-1127 -RRB- denotes ]
T2537 1127-1128 CD denotes 6
T2538 1128-1129 HYPH denotes -
T2539 1129-1130 CD denotes 3
T2540 1130-1131 SYM denotes ′
T2541 1131-1132 -RRB- denotes )
T2542 1133-1135 IN denotes at
T2543 1136-1139 DT denotes the
T2544 1146-1150 NN denotes site
T2545 1140-1145 NN denotes BamHI
T2546 1151-1153 IN denotes of
T2547 1154-1161 NN denotes M13mp19
T2548 1162-1165 CC denotes and
T2549 1166-1169 DT denotes the
T2550 1170-1179 NNS denotes sequences
T2551 1199-1208 VBN denotes confirmed
T2552 1180-1182 IN denotes of
T2553 1183-1186 DT denotes the
T2554 1187-1193 NN denotes insert
T2555 1194-1198 VBD denotes were
T2556 1208-1209 . denotes .
R1408 T2286 T2287 det The,substrates
R1409 T2287 T2290 nsubjpass substrates,prepared
R1410 T2288 T2287 amod partial,substrates
R1411 T2289 T2287 compound heteroduplex,substrates
R1412 T2291 T2287 prep on,substrates
R1413 T2292 T2293 det a,DNA
R1414 T2293 T2291 pobj DNA,on
R1415 T2294 T2295 amod single,stranded
R1416 T2295 T2293 amod stranded,DNA
R1417 T2296 T2295 punct -,stranded
R1418 T2297 T2293 amod circular,DNA
R1419 T2298 T2293 prep with,DNA
R1420 T2299 T2300 amod varied,lengths
R1421 T2300 T2298 pobj lengths,with
R1422 T2301 T2300 prep of,lengths
R1423 T2302 T2303 compound duplex,regions
R1424 T2303 T2301 pobj regions,of
R1425 T2304 T2290 auxpass were,prepared
R1426 T2305 T2290 prep by,prepared
R1427 T2306 T2305 pcomp extending,by
R1428 T2307 T2308 compound DNA,chains
R1429 T2308 T2306 dobj chains,extending
R1430 T2309 T2306 prep from,extending
R1431 T2310 T2311 det the,end
R1432 T2311 T2309 pobj end,from
R1433 T2312 T2311 nummod 3,end
R1434 T2313 T2312 punct ′,3
R1435 T2314 T2311 prep of,end
R1436 T2315 T2316 det the,37mer
R1437 T2316 T2314 pobj 37mer,of
R1438 T2317 T2316 compound dT40,37mer
R1439 T2318 T2316 punct -,37mer
R1440 T2319 T2316 punct (,37mer
R1441 T2320 T2321 compound 37mer,region
R1442 T2321 T2316 appos region,37mer
R1443 T2322 T2321 acl hybridizing,region
R1444 T2323 T2322 prep at,hybridizing
R1445 T2324 T2325 nmod positions,6289
R1446 T2325 T2323 pobj 6289,at
R1447 T2326 T2327 punct –,6326
R1448 T2327 T2325 prep 6326,6289
R1449 T2328 T2325 prep of,6289
R1450 T2329 T2330 compound M13mp18,vector
R1451 T2330 T2328 pobj vector,of
R1452 T2331 T2316 punct ),37mer
R1453 T2332 T2316 acl annealed,37mer
R1454 T2333 T2332 prep to,annealed
R1455 T2334 T2335 det the,DNA
R1456 T2335 T2333 pobj DNA,to
R1457 T2336 T2337 amod single,stranded
R1458 T2337 T2335 amod stranded,DNA
R1459 T2338 T2337 punct -,stranded
R1460 T2339 T2335 amod circular,DNA
R1461 T2340 T2290 punct .,prepared
R1462 T2342 T2343 compound DNA,chains
R1463 T2343 T2344 nsubjpass chains,elongated
R1464 T2345 T2344 auxpass were,elongated
R1465 T2346 T2344 prep with,elongated
R1466 T2347 T2346 pobj Sequenase,with
R1467 T2348 T2344 prep in,elongated
R1468 T2349 T2350 det the,presence
R1469 T2350 T2348 pobj presence,in
R1470 T2351 T2350 prep of,presence
R1471 T2352 T2353 punct [,dGTP
R1472 T2353 T2351 pobj dGTP,of
R1473 T2354 T2355 nmod α,32P
R1474 T2355 T2353 nmod 32P,dGTP
R1475 T2356 T2355 punct -,32P
R1476 T2357 T2353 punct ],dGTP
R1477 T2358 T2353 punct ", ",dGTP
R1478 T2359 T2353 acl followed,dGTP
R1479 T2360 T2359 agent by,followed
R1480 T2361 T2360 pobj extension,by
R1481 T2362 T2359 prep after,followed
R1482 T2363 T2362 pobj addition,after
R1483 T2364 T2363 prep of,addition
R1484 T2365 T2364 pobj ddGTP,of
R1485 T2366 T2365 cc and,ddGTP
R1486 T2367 T2368 det all,dNTPs
R1487 T2368 T2365 conj dNTPs,ddGTP
R1488 T2369 T2368 nummod four,dNTPs
R1489 T2370 T2344 punct ", ",elongated
R1490 T2371 T2344 advcl resulting,elongated
R1491 T2372 T2371 prep in,resulting
R1492 T2373 T2372 pobj substrates,in
R1493 T2374 T2373 acl containing,substrates
R1494 T2375 T2376 amod labeled,regions
R1495 T2376 T2374 dobj regions,containing
R1496 T2377 T2376 compound duplex,regions
R1497 T2378 T2376 prep of,regions
R1498 T2379 T2380 amod varied,lengths
R1499 T2380 T2378 pobj lengths,of
R1500 T2381 T2382 punct (,13
R1501 T2382 T2344 parataxis 13,elongated
R1502 T2383 T2382 punct ),13
R1503 T2384 T2344 punct .,elongated
R1504 T2386 T2387 det The,substrate
R1505 T2387 T2388 nsubjpass substrate,incubated
R1506 T2389 T2390 punct (,fmol
R1507 T2390 T2387 parataxis fmol,substrate
R1508 T2391 T2390 nummod 5,fmol
R1509 T2392 T2390 punct ),fmol
R1510 T2393 T2388 auxpass was,incubated
R1511 T2394 T2388 advmod first,incubated
R1512 T2395 T2388 prep with,incubated
R1513 T2396 T2397 amod indicated,amount
R1514 T2397 T2395 pobj amount,with
R1515 T2398 T2397 prep of,amount
R1516 T2399 T2400 det the,complex
R1517 T2400 T2398 pobj complex,of
R1518 T2401 T2400 nmod Mcm4,complex
R1519 T2402 T2401 punct /,Mcm4
R1520 T2403 T2401 nummod 6,Mcm4
R1521 T2404 T2401 punct /,Mcm4
R1522 T2405 T2401 nummod 7,Mcm4
R1523 T2406 T2388 prep at,incubated
R1524 T2407 T2408 nummod 37,°C
R1525 T2408 T2406 pobj °C,at
R1526 T2409 T2388 prep for,incubated
R1527 T2410 T2411 nummod 1,h
R1528 T2411 T2409 pobj h,for
R1529 T2412 T2388 prep in,incubated
R1530 T2413 T2414 compound reaction,mixture
R1531 T2414 T2412 pobj mixture,in
R1532 T2415 T2416 mark as,described
R1533 T2416 T2388 advcl described,incubated
R1534 T2417 T2418 punct (,11
R1535 T2418 T2416 parataxis 11,described
R1536 T2419 T2418 punct ),11
R1537 T2420 T2388 punct ", ",incubated
R1538 T2421 T2388 cc and,incubated
R1539 T2422 T2421 advmod then,and
R1540 T2423 T2424 nsubjpass reactions,stopped
R1541 T2424 T2388 conj stopped,incubated
R1542 T2425 T2424 auxpass were,stopped
R1543 T2426 T2424 agent by,stopped
R1544 T2427 T2426 pobj addition,by
R1545 T2428 T2427 prep of,addition
R1546 T2429 T2428 pobj EDTA,of
R1547 T2430 T2431 punct (,mM
R1548 T2431 T2429 parataxis mM,EDTA
R1549 T2432 T2431 nummod 20,mM
R1550 T2433 T2431 punct ),mM
R1551 T2434 T2429 punct ", ",EDTA
R1552 T2435 T2429 conj SDS,EDTA
R1553 T2436 T2437 punct (,%
R1554 T2437 T2435 parataxis %,SDS
R1555 T2438 T2437 nummod 0.1,%
R1556 T2439 T2437 punct ),%
R1557 T2440 T2435 cc and,SDS
R1558 T2441 T2442 nummod 2,μg
R1559 T2442 T2443 compound μg,K
R1560 T2443 T2435 conj K,SDS
R1561 T2444 T2443 compound proteinase,K
R1562 T2445 T2427 punct ", ",addition
R1563 T2446 T2427 acl followed,addition
R1564 T2447 T2446 agent by,followed
R1565 T2448 T2447 pobj incubation,by
R1566 T2449 T2448 prep for,incubation
R1567 T2450 T2451 nummod 20,min
R1568 T2451 T2449 pobj min,for
R1569 T2452 T2424 punct .,stopped
R1570 T2454 T2455 nsubjpass Aliquots,electrophoresed
R1571 T2456 T2455 auxpass were,electrophoresed
R1572 T2457 T2455 prep on,electrophoresed
R1573 T2458 T2459 det a,gel
R1574 T2459 T2457 pobj gel,on
R1575 T2460 T2461 nummod 6.5,%
R1576 T2461 T2459 compound %,gel
R1577 T2462 T2459 compound polyacrylamide,gel
R1578 T2463 T2455 prep in,electrophoresed
R1579 T2464 T2465 nummod 1,TBE
R1580 T2465 T2463 pobj TBE,in
R1581 T2466 T2464 punct ×,1
R1582 T2467 T2455 prep at,electrophoresed
R1583 T2468 T2469 nummod 300,V
R1584 T2469 T2467 pobj V,at
R1585 T2470 T2455 prep for,electrophoresed
R1586 T2471 T2472 nummod 2.5,h
R1587 T2472 T2470 pobj h,for
R1588 T2473 T2455 punct .,electrophoresed
R1589 T2475 T2476 det The,DNAs
R1590 T2476 T2481 nsubj DNAs,were
R1591 T2477 T2478 amod single,stranded
R1592 T2478 T2476 amod stranded,DNAs
R1593 T2479 T2478 punct -,stranded
R1594 T2480 T2476 amod circular,DNAs
R1595 T2482 T2476 acl used,DNAs
R1596 T2483 T2481 attr M13mp18,were
R1597 T2484 T2483 cc and,M13mp18
R1598 T2485 T2483 conj M13mp19,M13mp18
R1599 T2486 T2483 punct +,M13mp18
R1600 T2487 T2483 appos G,M13mp18
R1601 T2488 T2487 punct -,G
R1602 T2489 T2487 amod rich,G
R1603 T2490 T2481 punct .,were
R1604 T2492 T2493 det The,latter
R1605 T2493 T2494 nsubjpass latter,constructed
R1606 T2495 T2494 auxpass was,constructed
R1607 T2496 T2494 prep by,constructed
R1608 T2497 T2496 pcomp inserting,by
R1609 T2498 T2499 det a,sequence
R1610 T2499 T2497 dobj sequence,inserting
R1611 T2500 T2499 nmod G,sequence
R1612 T2501 T2500 punct -,G
R1613 T2502 T2500 amod rich,G
R1614 T2503 T2499 compound repeat,sequence
R1615 T2504 T2499 punct (,sequence
R1616 T2505 T2499 appos GGGGCGTGGGC,sequence
R1617 T2506 T2505 punct ),GGGGCGTGGGC
R1618 T2507 T2505 nummod 6,GGGGCGTGGGC
R1619 T2508 T2499 punct ", ",sequence
R1620 T2509 T2499 acl prepared,sequence
R1621 T2510 T2509 prep by,prepared
R1622 T2511 T2510 pcomp annealing,by
R1623 T2512 T2511 prep of,annealing
R1624 T2513 T2514 nummod two,oligonucleotides
R1625 T2514 T2512 pobj oligonucleotides,of
R1626 T2515 T2514 punct (,oligonucleotides
R1627 T2516 T2514 appos 5,oligonucleotides
R1628 T2517 T2516 punct ′,5
R1629 T2518 T2516 punct -,5
R1630 T2519 T2520 nmod GATCC,GGGGCGTGGGC
R1631 T2520 T2516 appos GGGGCGTGGGC,5
R1632 T2521 T2520 punct -,GGGGCGTGGGC
R1633 T2522 T2520 punct [,GGGGCGTGGGC
R1634 T2523 T2520 punct ],GGGGCGTGGGC
R1635 T2524 T2520 nummod 6,GGGGCGTGGGC
R1636 T2525 T2516 punct -,5
R1637 T2526 T2516 nummod 3,5
R1638 T2527 T2516 punct ′,5
R1639 T2528 T2516 punct /,5
R1640 T2529 T2516 appos 5,5
R1641 T2530 T2529 punct ′,5
R1642 T2531 T2529 punct -,5
R1643 T2532 T2533 nmod GATCC,GCCCACGCCCC
R1644 T2533 T2529 appos GCCCACGCCCC,5
R1645 T2534 T2533 punct -,GCCCACGCCCC
R1646 T2535 T2533 punct [,GCCCACGCCCC
R1647 T2536 T2533 punct ],GCCCACGCCCC
R1648 T2537 T2533 nummod 6,GCCCACGCCCC
R1649 T2538 T2529 punct -,5
R1650 T2539 T2529 nummod 3,5
R1651 T2540 T2529 punct ′,5
R1652 T2541 T2514 punct ),oligonucleotides
R1653 T2542 T2497 prep at,inserting
R1654 T2543 T2544 det the,site
R1655 T2544 T2542 pobj site,at
R1656 T2545 T2544 compound BamHI,site
R1657 T2546 T2544 prep of,site
R1658 T2547 T2546 pobj M13mp19,of
R1659 T2548 T2494 cc and,constructed
R1660 T2549 T2550 det the,sequences
R1661 T2550 T2551 nsubjpass sequences,confirmed
R1662 T2551 T2494 conj confirmed,constructed
R1663 T2552 T2550 prep of,sequences
R1664 T2553 T2554 det the,insert
R1665 T2554 T2552 pobj insert,of
R1666 T2555 T2551 auxpass were,confirmed
R1667 T2556 T2551 punct .,confirmed

craft-ca-core-ex-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T1942 18-24 SO:0000985 denotes duplex
T1943 41-56 _FRAGMENT denotes single-stranded
T1944 66-69 CHEBI_SO_EXT:single_stranded_DNA denotes DNA
T1945 57-65 SO:0000988 denotes circular
T1946 93-99 SO:0000985 denotes duplex
T1947 135-138 CHEBI_SO_EXT:DNA denotes DNA
T1948 194-205 GO:0097617 denotes hybridizing
T1949 240-246 SO_EXT:0000440 denotes vector
T1950 248-256 GO:0097617 denotes annealed
T1951 264-279 _FRAGMENT denotes single-stranded
T1952 289-292 CHEBI_SO_EXT:single_stranded_DNA denotes DNA
T1953 280-288 SO:0000988 denotes circular
T1954 294-297 CHEBI_SO_EXT:DNA denotes DNA
T1955 357-360 CHEBI:37972 denotes 32P
T1956 361-365 CHEBI_EXT:dGTP denotes dGTP
T1957 426-431 CHEBI_EXT:dNTP denotes dNTPs
T1958 468-475 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes labeled
T1959 476-482 SO:0000985 denotes duplex
T1960 587-591 PR_EXT:000010246 denotes Mcm4
T1961 587-603 GO:0097373 denotes Mcm4/6/7 complex
T1962 632-639 CHEBI:60004 denotes mixture
T1963 706-710 CHEBI_EXT:EDTA denotes EDTA
T1964 720-723 CHEBI:8984 denotes SDS
T1965 740-750 GO_EXT:0008233 denotes proteinase
T1966 829-843 CHEBI_EXT:polyacrylamide denotes polyacrylamide
T1967 882-897 _FRAGMENT denotes single-stranded
T1968 907-911 CHEBI_SO_EXT:single_stranded_DNA denotes DNAs
T1969 898-906 SO:0000988 denotes circular
T1970 942-943 CHEBI_EXT:guanine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group denotes G
T1971 980-989 SO_EXT:sequence_insertion_process denotes inserting
T1972 992-993 CHEBI_EXT:guanine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group denotes G
T1973 999-1005 SO_EXT:sequence_repeat_unit_or_region denotes repeat
T1974 1006-1014 SO_EXT:biological_sequence denotes sequence
T1975 1043-1052 GO:0097617 denotes annealing
T1976 1060-1076 CHEBI_SO_EXT:oligonucleotide denotes oligonucleotides
T1977 1140-1145 PR_EXT:P23940 denotes BamHI
T1978 1170-1179 SO_EXT:biological_sequence denotes sequences
T1979 1187-1193 SO_EXT:0000667 denotes insert
R1121 T1944 T1943 _lexicallyChainedTo DNA,single-stranded
R1122 T1952 T1951 _lexicallyChainedTo DNA,single-stranded
R1123 T1968 T1967 _lexicallyChainedTo DNAs,single-stranded

craft-ca-core-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T1877 18-24 SO:0000985 denotes duplex
T1878 41-56 SO:0000984 denotes single-stranded
T1879 57-65 SO:0000988 denotes circular
T1880 93-99 SO:0000985 denotes duplex
T1881 194-205 GO:0097617 denotes hybridizing
T1882 240-246 SO:0000440 denotes vector
T1883 248-256 GO:0097617 denotes annealed
T1884 264-279 SO:0000984 denotes single-stranded
T1885 280-288 SO:0000988 denotes circular
T1886 357-360 CHEBI:37972 denotes 32P
T1887 476-482 SO:0000985 denotes duplex
T1888 587-591 PR:000010246 denotes Mcm4
T1889 587-603 GO:0097373 denotes Mcm4/6/7 complex
T1890 632-639 CHEBI:60004 denotes mixture
T1891 720-723 CHEBI:8984 denotes SDS
T1892 882-897 SO:0000984 denotes single-stranded
T1893 898-906 SO:0000988 denotes circular
T1894 1043-1052 GO:0097617 denotes annealing
T1895 1140-1145 PR:P23940 denotes BamHI
T1896 1187-1193 SO:0000667 denotes insert

2_test

Id Subject Object Predicate Lexical cue
15917436-14609960-76775506 510-512 14609960 denotes 13
15917436-10567526-76775507 654-656 10567526 denotes 11