Id |
Subject |
Object |
Predicate |
Lexical cue |
T157 |
0-77 |
sentence |
denotes |
Helicases play central roles in initiation and elongation of DNA replication. |
T158 |
0-9 |
NNS |
denotes |
Helicases |
T159 |
10-14 |
VBP |
denotes |
play |
T160 |
15-22 |
JJ |
denotes |
central |
T161 |
23-28 |
NNS |
denotes |
roles |
T162 |
29-31 |
IN |
denotes |
in |
T163 |
32-42 |
NN |
denotes |
initiation |
T164 |
43-46 |
CC |
denotes |
and |
T165 |
47-57 |
NN |
denotes |
elongation |
T166 |
58-60 |
IN |
denotes |
of |
T167 |
61-64 |
NN |
denotes |
DNA |
T168 |
65-76 |
NN |
denotes |
replication |
T169 |
76-77 |
. |
denotes |
. |
T170 |
77-234 |
sentence |
denotes |
We previously reported that helicase and ATPase activities of the mammalian Mcm4/6/7 complex are activated specifically by thymine-rich single-stranded DNA. |
T171 |
78-80 |
PRP |
denotes |
We |
T172 |
92-100 |
VBD |
denotes |
reported |
T173 |
81-91 |
RB |
denotes |
previously |
T174 |
101-105 |
IN |
denotes |
that |
T175 |
175-184 |
VBN |
denotes |
activated |
T176 |
106-114 |
NN |
denotes |
helicase |
T177 |
126-136 |
NNS |
denotes |
activities |
T178 |
115-118 |
CC |
denotes |
and |
T179 |
119-125 |
NN |
denotes |
ATPase |
T180 |
137-139 |
IN |
denotes |
of |
T181 |
140-143 |
DT |
denotes |
the |
T182 |
163-170 |
NN |
denotes |
complex |
T183 |
144-153 |
JJ |
denotes |
mammalian |
T184 |
154-158 |
NN |
denotes |
Mcm4 |
T185 |
158-159 |
HYPH |
denotes |
/ |
T186 |
159-160 |
CD |
denotes |
6 |
T187 |
160-161 |
HYPH |
denotes |
/ |
T188 |
161-162 |
CD |
denotes |
7 |
T189 |
171-174 |
VBP |
denotes |
are |
T190 |
185-197 |
RB |
denotes |
specifically |
T191 |
198-200 |
IN |
denotes |
by |
T192 |
201-208 |
NN |
denotes |
thymine |
T193 |
209-213 |
JJ |
denotes |
rich |
T194 |
208-209 |
HYPH |
denotes |
- |
T195 |
230-233 |
NN |
denotes |
DNA |
T196 |
214-220 |
JJ |
denotes |
single |
T197 |
221-229 |
VBN |
denotes |
stranded |
T198 |
220-221 |
HYPH |
denotes |
- |
T199 |
233-234 |
. |
denotes |
. |
T200 |
234-328 |
sentence |
denotes |
Here, we examined its substrate preference and helicase actions using various synthetic DNAs. |
T201 |
235-239 |
RB |
denotes |
Here |
T202 |
244-252 |
VBD |
denotes |
examined |
T203 |
239-241 |
, |
denotes |
, |
T204 |
241-243 |
PRP |
denotes |
we |
T205 |
253-256 |
PRP$ |
denotes |
its |
T206 |
267-277 |
NN |
denotes |
preference |
T207 |
257-266 |
NN |
denotes |
substrate |
T208 |
278-281 |
CC |
denotes |
and |
T209 |
282-290 |
NN |
denotes |
helicase |
T210 |
291-298 |
NNS |
denotes |
actions |
T211 |
299-304 |
VBG |
denotes |
using |
T212 |
305-312 |
JJ |
denotes |
various |
T213 |
323-327 |
NNS |
denotes |
DNAs |
T214 |
313-322 |
JJ |
denotes |
synthetic |
T215 |
327-328 |
. |
denotes |
. |
T216 |
328-512 |
sentence |
denotes |
On a bubble substrate, Mcm4/6/7 makes symmetric dual contacts with the 5′-proximal 25 nt single-stranded segments adjacent to the branch points, presumably generating double hexamers. |
T217 |
329-331 |
IN |
denotes |
On |
T218 |
361-366 |
VBZ |
denotes |
makes |
T219 |
332-333 |
DT |
denotes |
a |
T220 |
341-350 |
NN |
denotes |
substrate |
T221 |
334-340 |
NN |
denotes |
bubble |
T222 |
350-352 |
, |
denotes |
, |
T223 |
352-356 |
NN |
denotes |
Mcm4 |
T224 |
356-357 |
HYPH |
denotes |
/ |
T225 |
357-358 |
CD |
denotes |
6 |
T226 |
358-359 |
HYPH |
denotes |
/ |
T227 |
359-360 |
CD |
denotes |
7 |
T228 |
367-376 |
JJ |
denotes |
symmetric |
T229 |
382-390 |
NNS |
denotes |
contacts |
T230 |
377-381 |
JJ |
denotes |
dual |
T231 |
391-395 |
IN |
denotes |
with |
T232 |
396-399 |
DT |
denotes |
the |
T233 |
434-442 |
NNS |
denotes |
segments |
T234 |
400-401 |
CD |
denotes |
5 |
T235 |
403-411 |
JJ |
denotes |
proximal |
T236 |
401-402 |
SYM |
denotes |
′ |
T237 |
402-403 |
HYPH |
denotes |
- |
T238 |
412-414 |
CD |
denotes |
25 |
T239 |
415-417 |
NN |
denotes |
nt |
T240 |
418-424 |
JJ |
denotes |
single |
T241 |
425-433 |
VBN |
denotes |
stranded |
T242 |
424-425 |
HYPH |
denotes |
- |
T243 |
443-451 |
JJ |
denotes |
adjacent |
T244 |
452-454 |
IN |
denotes |
to |
T245 |
455-458 |
DT |
denotes |
the |
T246 |
466-472 |
NNS |
denotes |
points |
T247 |
459-465 |
NN |
denotes |
branch |
T248 |
472-474 |
, |
denotes |
, |
T249 |
474-484 |
RB |
denotes |
presumably |
T250 |
485-495 |
VBG |
denotes |
generating |
T251 |
496-502 |
JJ |
denotes |
double |
T252 |
503-511 |
NNS |
denotes |
hexamers |
T253 |
511-512 |
. |
denotes |
. |
T254 |
512-758 |
sentence |
denotes |
Loss of thymine residues from one single-strand results in significant decrease of unwinding efficacy, suggesting that concurrent bidirectional unwinding by a single double hexameric Mcm4/6/7 may play a role in efficient unwinding of the bubble. |
T255 |
513-517 |
NN |
denotes |
Loss |
T256 |
561-568 |
VBZ |
denotes |
results |
T257 |
518-520 |
IN |
denotes |
of |
T258 |
521-528 |
NN |
denotes |
thymine |
T259 |
529-537 |
NNS |
denotes |
residues |
T260 |
538-542 |
IN |
denotes |
from |
T261 |
543-546 |
CD |
denotes |
one |
T262 |
554-560 |
NN |
denotes |
strand |
T263 |
547-553 |
JJ |
denotes |
single |
T264 |
553-554 |
HYPH |
denotes |
- |
T265 |
569-571 |
IN |
denotes |
in |
T266 |
572-583 |
JJ |
denotes |
significant |
T267 |
584-592 |
NN |
denotes |
decrease |
T268 |
593-595 |
IN |
denotes |
of |
T269 |
596-605 |
VBG |
denotes |
unwinding |
T270 |
606-614 |
NN |
denotes |
efficacy |
T271 |
614-616 |
, |
denotes |
, |
T272 |
616-626 |
VBG |
denotes |
suggesting |
T273 |
627-631 |
IN |
denotes |
that |
T274 |
709-713 |
VB |
denotes |
play |
T275 |
632-642 |
JJ |
denotes |
concurrent |
T276 |
657-666 |
NN |
denotes |
unwinding |
T277 |
643-656 |
JJ |
denotes |
bidirectional |
T278 |
667-669 |
IN |
denotes |
by |
T279 |
670-671 |
DT |
denotes |
a |
T280 |
696-700 |
NN |
denotes |
Mcm4 |
T281 |
672-678 |
JJ |
denotes |
single |
T282 |
679-685 |
JJ |
denotes |
double |
T283 |
686-695 |
JJ |
denotes |
hexameric |
T284 |
700-701 |
HYPH |
denotes |
/ |
T285 |
701-702 |
CD |
denotes |
6 |
T286 |
702-703 |
HYPH |
denotes |
/ |
T287 |
703-704 |
CD |
denotes |
7 |
T288 |
705-708 |
MD |
denotes |
may |
T289 |
714-715 |
DT |
denotes |
a |
T290 |
716-720 |
NN |
denotes |
role |
T291 |
721-723 |
IN |
denotes |
in |
T292 |
724-733 |
JJ |
denotes |
efficient |
T293 |
734-743 |
NN |
denotes |
unwinding |
T294 |
744-746 |
IN |
denotes |
of |
T295 |
747-750 |
DT |
denotes |
the |
T296 |
751-757 |
NN |
denotes |
bubble |
T297 |
757-758 |
. |
denotes |
. |
T298 |
758-863 |
sentence |
denotes |
Mcm4/6/7 binds and unwinds various fork and extension structures carrying a single-stranded 3′-tail DNA. |
T299 |
759-763 |
NN |
denotes |
Mcm4 |
T300 |
768-773 |
VBZ |
denotes |
binds |
T301 |
763-764 |
HYPH |
denotes |
/ |
T302 |
764-765 |
CD |
denotes |
6 |
T303 |
765-766 |
HYPH |
denotes |
/ |
T304 |
766-767 |
CD |
denotes |
7 |
T305 |
774-777 |
CC |
denotes |
and |
T306 |
778-785 |
VBZ |
denotes |
unwinds |
T307 |
786-793 |
JJ |
denotes |
various |
T308 |
813-823 |
NNS |
denotes |
structures |
T309 |
794-798 |
NN |
denotes |
fork |
T310 |
799-802 |
CC |
denotes |
and |
T311 |
803-812 |
NN |
denotes |
extension |
T312 |
824-832 |
VBG |
denotes |
carrying |
T313 |
833-834 |
DT |
denotes |
a |
T314 |
859-862 |
NN |
denotes |
DNA |
T315 |
835-841 |
JJ |
denotes |
single |
T316 |
842-850 |
VBN |
denotes |
stranded |
T317 |
841-842 |
HYPH |
denotes |
- |
T318 |
851-852 |
CD |
denotes |
3 |
T319 |
854-858 |
NN |
denotes |
tail |
T320 |
852-853 |
SYM |
denotes |
′ |
T321 |
853-854 |
HYPH |
denotes |
- |
T322 |
862-863 |
. |
denotes |
. |
T323 |
863-1020 |
sentence |
denotes |
The extent of helicase activation depends on the sequence context of the 3′-tail, and the maximum level is achieved by DNA with 50% or more thymine content. |
T324 |
864-867 |
DT |
denotes |
The |
T325 |
868-874 |
NN |
denotes |
extent |
T326 |
898-905 |
VBZ |
denotes |
depends |
T327 |
875-877 |
IN |
denotes |
of |
T328 |
878-886 |
NN |
denotes |
helicase |
T329 |
887-897 |
NN |
denotes |
activation |
T330 |
906-908 |
IN |
denotes |
on |
T331 |
909-912 |
DT |
denotes |
the |
T332 |
922-929 |
NN |
denotes |
context |
T333 |
913-921 |
NN |
denotes |
sequence |
T334 |
930-932 |
IN |
denotes |
of |
T335 |
933-936 |
DT |
denotes |
the |
T336 |
940-944 |
NN |
denotes |
tail |
T337 |
937-938 |
CD |
denotes |
3 |
T338 |
938-939 |
SYM |
denotes |
′ |
T339 |
939-940 |
HYPH |
denotes |
- |
T340 |
944-946 |
, |
denotes |
, |
T341 |
946-949 |
CC |
denotes |
and |
T342 |
950-953 |
DT |
denotes |
the |
T343 |
962-967 |
NN |
denotes |
level |
T344 |
954-961 |
JJ |
denotes |
maximum |
T345 |
971-979 |
VBN |
denotes |
achieved |
T346 |
968-970 |
VBZ |
denotes |
is |
T347 |
980-982 |
IN |
denotes |
by |
T348 |
983-986 |
NN |
denotes |
DNA |
T349 |
987-991 |
IN |
denotes |
with |
T350 |
992-994 |
CD |
denotes |
50 |
T351 |
994-995 |
NN |
denotes |
% |
T352 |
1012-1019 |
NN |
denotes |
content |
T353 |
996-998 |
CC |
denotes |
or |
T354 |
999-1003 |
JJR |
denotes |
more |
T355 |
1004-1011 |
NN |
denotes |
thymine |
T356 |
1019-1020 |
. |
denotes |
. |
T357 |
1020-1116 |
sentence |
denotes |
Strand displacement by Mcm4/6/7 is inhibited, as the GC content of the duplex region increases. |
T358 |
1021-1027 |
NN |
denotes |
Strand |
T359 |
1028-1040 |
NN |
denotes |
displacement |
T360 |
1056-1065 |
VBN |
denotes |
inhibited |
T361 |
1041-1043 |
IN |
denotes |
by |
T362 |
1044-1048 |
NN |
denotes |
Mcm4 |
T363 |
1048-1049 |
HYPH |
denotes |
/ |
T364 |
1049-1050 |
CD |
denotes |
6 |
T365 |
1050-1051 |
HYPH |
denotes |
/ |
T366 |
1051-1052 |
CD |
denotes |
7 |
T367 |
1053-1055 |
VBZ |
denotes |
is |
T368 |
1065-1067 |
, |
denotes |
, |
T369 |
1067-1069 |
IN |
denotes |
as |
T370 |
1106-1115 |
VBZ |
denotes |
increases |
T371 |
1070-1073 |
DT |
denotes |
the |
T372 |
1077-1084 |
NN |
denotes |
content |
T373 |
1074-1076 |
NN |
denotes |
GC |
T374 |
1085-1087 |
IN |
denotes |
of |
T375 |
1088-1091 |
DT |
denotes |
the |
T376 |
1099-1105 |
NN |
denotes |
region |
T377 |
1092-1098 |
NN |
denotes |
duplex |
T378 |
1115-1116 |
. |
denotes |
. |
T379 |
1116-1317 |
sentence |
denotes |
Replacement of cytosine–guanine pairs with cytosine–inosine pairs in the duplex restored unwinding, suggesting that mammalian Mcm4/6/7 helicase has difficulties in unwinding stably base-paired duplex. |
T380 |
1117-1128 |
NN |
denotes |
Replacement |
T381 |
1197-1205 |
VBD |
denotes |
restored |
T382 |
1129-1131 |
IN |
denotes |
of |
T383 |
1132-1140 |
NN |
denotes |
cytosine |
T384 |
1141-1148 |
NN |
denotes |
guanine |
T385 |
1140-1141 |
HYPH |
denotes |
– |
T386 |
1149-1154 |
NNS |
denotes |
pairs |
T387 |
1155-1159 |
IN |
denotes |
with |
T388 |
1160-1168 |
NN |
denotes |
cytosine |
T389 |
1169-1176 |
NN |
denotes |
inosine |
T390 |
1168-1169 |
HYPH |
denotes |
– |
T391 |
1177-1182 |
NNS |
denotes |
pairs |
T392 |
1183-1185 |
IN |
denotes |
in |
T393 |
1186-1189 |
DT |
denotes |
the |
T394 |
1190-1196 |
NN |
denotes |
duplex |
T395 |
1206-1215 |
NN |
denotes |
unwinding |
T396 |
1215-1217 |
, |
denotes |
, |
T397 |
1217-1227 |
VBG |
denotes |
suggesting |
T398 |
1228-1232 |
IN |
denotes |
that |
T399 |
1261-1264 |
VBZ |
denotes |
has |
T400 |
1233-1242 |
JJ |
denotes |
mammalian |
T401 |
1252-1260 |
NN |
denotes |
helicase |
T402 |
1243-1247 |
NN |
denotes |
Mcm4 |
T403 |
1247-1248 |
HYPH |
denotes |
/ |
T404 |
1248-1249 |
CD |
denotes |
6 |
T405 |
1249-1250 |
HYPH |
denotes |
/ |
T406 |
1250-1251 |
CD |
denotes |
7 |
T407 |
1265-1277 |
NNS |
denotes |
difficulties |
T408 |
1278-1280 |
IN |
denotes |
in |
T409 |
1281-1290 |
VBG |
denotes |
unwinding |
T410 |
1291-1297 |
RB |
denotes |
stably |
T411 |
1303-1309 |
VBN |
denotes |
paired |
T412 |
1298-1302 |
NN |
denotes |
base |
T413 |
1302-1303 |
HYPH |
denotes |
- |
T414 |
1310-1316 |
NN |
denotes |
duplex |
T415 |
1316-1317 |
. |
denotes |
. |
T416 |
1317-1453 |
sentence |
denotes |
Taken together, these findings reveal important features on activation and substrate preference of the eukaryotic replicative helicase. |
T417 |
1318-1323 |
VBN |
denotes |
Taken |
T418 |
1349-1355 |
VBP |
denotes |
reveal |
T419 |
1324-1332 |
RB |
denotes |
together |
T420 |
1332-1334 |
, |
denotes |
, |
T421 |
1334-1339 |
DT |
denotes |
these |
T422 |
1340-1348 |
NNS |
denotes |
findings |
T423 |
1356-1365 |
JJ |
denotes |
important |
T424 |
1366-1374 |
NNS |
denotes |
features |
T425 |
1375-1377 |
IN |
denotes |
on |
T426 |
1378-1388 |
NN |
denotes |
activation |
T427 |
1403-1413 |
NN |
denotes |
preference |
T428 |
1389-1392 |
CC |
denotes |
and |
T429 |
1393-1402 |
NN |
denotes |
substrate |
T430 |
1414-1416 |
IN |
denotes |
of |
T431 |
1417-1420 |
DT |
denotes |
the |
T432 |
1444-1452 |
NN |
denotes |
helicase |
T433 |
1421-1431 |
JJ |
denotes |
eukaryotic |
T434 |
1432-1443 |
JJ |
denotes |
replicative |
T435 |
1452-1453 |
. |
denotes |
. |
R15 |
T158 |
T159 |
nsubj |
Helicases,play |
R16 |
T160 |
T161 |
amod |
central,roles |
R17 |
T161 |
T159 |
dobj |
roles,play |
R18 |
T162 |
T159 |
prep |
in,play |
R19 |
T163 |
T162 |
pobj |
initiation,in |
R20 |
T164 |
T163 |
cc |
and,initiation |
R21 |
T165 |
T163 |
conj |
elongation,initiation |
R22 |
T166 |
T163 |
prep |
of,initiation |
R23 |
T167 |
T168 |
compound |
DNA,replication |
R24 |
T168 |
T166 |
pobj |
replication,of |
R25 |
T169 |
T159 |
punct |
.,play |
R26 |
T171 |
T172 |
nsubj |
We,reported |
R27 |
T173 |
T172 |
advmod |
previously,reported |
R28 |
T174 |
T175 |
mark |
that,activated |
R29 |
T175 |
T172 |
ccomp |
activated,reported |
R30 |
T176 |
T177 |
nmod |
helicase,activities |
R31 |
T177 |
T175 |
nsubjpass |
activities,activated |
R32 |
T178 |
T176 |
cc |
and,helicase |
R33 |
T179 |
T176 |
conj |
ATPase,helicase |
R34 |
T180 |
T177 |
prep |
of,activities |
R35 |
T181 |
T182 |
det |
the,complex |
R36 |
T182 |
T180 |
pobj |
complex,of |
R37 |
T183 |
T182 |
amod |
mammalian,complex |
R38 |
T184 |
T182 |
nmod |
Mcm4,complex |
R39 |
T185 |
T184 |
punct |
/,Mcm4 |
R40 |
T291 |
T274 |
prep |
in,play |
R41 |
T292 |
T293 |
amod |
efficient,unwinding |
R42 |
T293 |
T291 |
pobj |
unwinding,in |
R43 |
T294 |
T293 |
prep |
of,unwinding |
R44 |
T295 |
T296 |
det |
the,bubble |
R45 |
T296 |
T294 |
pobj |
bubble,of |
R46 |
T297 |
T256 |
punct |
.,results |
R47 |
T299 |
T300 |
nsubj |
Mcm4,binds |
R48 |
T301 |
T299 |
punct |
/,Mcm4 |
R49 |
T302 |
T299 |
nummod |
6,Mcm4 |
R50 |
T303 |
T299 |
punct |
/,Mcm4 |
R51 |
T186 |
T184 |
nummod |
6,Mcm4 |
R52 |
T187 |
T184 |
punct |
/,Mcm4 |
R53 |
T188 |
T184 |
nummod |
7,Mcm4 |
R54 |
T304 |
T299 |
nummod |
7,Mcm4 |
R55 |
T189 |
T175 |
auxpass |
are,activated |
R56 |
T190 |
T175 |
advmod |
specifically,activated |
R57 |
T191 |
T175 |
agent |
by,activated |
R58 |
T305 |
T300 |
cc |
and,binds |
R59 |
T192 |
T193 |
npadvmod |
thymine,rich |
R60 |
T193 |
T195 |
amod |
rich,DNA |
R61 |
T306 |
T300 |
conj |
unwinds,binds |
R62 |
T194 |
T193 |
punct |
-,rich |
R63 |
T307 |
T308 |
amod |
various,structures |
R64 |
T308 |
T306 |
dobj |
structures,unwinds |
R65 |
T309 |
T308 |
nmod |
fork,structures |
R66 |
T310 |
T309 |
cc |
and,fork |
R67 |
T195 |
T191 |
pobj |
DNA,by |
R68 |
T311 |
T309 |
conj |
extension,fork |
R69 |
T196 |
T197 |
amod |
single,stranded |
R70 |
T197 |
T195 |
amod |
stranded,DNA |
R71 |
T312 |
T308 |
acl |
carrying,structures |
R72 |
T198 |
T197 |
punct |
-,stranded |
R73 |
T199 |
T172 |
punct |
.,reported |
R74 |
T313 |
T314 |
det |
a,DNA |
R75 |
T201 |
T202 |
advmod |
Here,examined |
R76 |
T314 |
T312 |
dobj |
DNA,carrying |
R77 |
T203 |
T202 |
punct |
", ",examined |
R78 |
T315 |
T316 |
amod |
single,stranded |
R79 |
T204 |
T202 |
nsubj |
we,examined |
R80 |
T205 |
T206 |
poss |
its,preference |
R81 |
T316 |
T314 |
amod |
stranded,DNA |
R82 |
T206 |
T202 |
dobj |
preference,examined |
R83 |
T207 |
T206 |
compound |
substrate,preference |
R84 |
T208 |
T206 |
cc |
and,preference |
R85 |
T317 |
T316 |
punct |
-,stranded |
R86 |
T209 |
T210 |
compound |
helicase,actions |
R87 |
T210 |
T206 |
conj |
actions,preference |
R88 |
T318 |
T319 |
nummod |
3,tail |
R89 |
T211 |
T202 |
advcl |
using,examined |
R90 |
T212 |
T213 |
amod |
various,DNAs |
R91 |
T319 |
T314 |
compound |
tail,DNA |
R92 |
T213 |
T211 |
dobj |
DNAs,using |
R93 |
T214 |
T213 |
amod |
synthetic,DNAs |
R94 |
T320 |
T318 |
punct |
′,3 |
R95 |
T215 |
T202 |
punct |
.,examined |
R96 |
T217 |
T218 |
prep |
On,makes |
R97 |
T321 |
T319 |
punct |
-,tail |
R98 |
T219 |
T220 |
det |
a,substrate |
R99 |
T322 |
T300 |
punct |
.,binds |
R100 |
T220 |
T217 |
pobj |
substrate,On |
R101 |
T324 |
T325 |
det |
The,extent |
R102 |
T221 |
T220 |
compound |
bubble,substrate |
R103 |
T222 |
T218 |
punct |
", ",makes |
R104 |
T223 |
T218 |
nsubj |
Mcm4,makes |
R105 |
T224 |
T223 |
punct |
/,Mcm4 |
R106 |
T225 |
T223 |
nummod |
6,Mcm4 |
R107 |
T226 |
T223 |
punct |
/,Mcm4 |
R108 |
T325 |
T326 |
nsubj |
extent,depends |
R109 |
T227 |
T223 |
nummod |
7,Mcm4 |
R110 |
T228 |
T229 |
amod |
symmetric,contacts |
R111 |
T327 |
T325 |
prep |
of,extent |
R112 |
T229 |
T218 |
dobj |
contacts,makes |
R113 |
T230 |
T229 |
amod |
dual,contacts |
R114 |
T231 |
T229 |
prep |
with,contacts |
R115 |
T328 |
T329 |
compound |
helicase,activation |
R116 |
T232 |
T233 |
det |
the,segments |
R117 |
T233 |
T231 |
pobj |
segments,with |
R118 |
T329 |
T327 |
pobj |
activation,of |
R119 |
T234 |
T235 |
npadvmod |
5,proximal |
R120 |
T235 |
T233 |
amod |
proximal,segments |
R121 |
T236 |
T234 |
punct |
′,5 |
R122 |
T237 |
T235 |
punct |
-,proximal |
R123 |
T238 |
T239 |
nummod |
25,nt |
R124 |
T330 |
T326 |
prep |
on,depends |
R125 |
T331 |
T332 |
det |
the,context |
R126 |
T239 |
T233 |
nmod |
nt,segments |
R127 |
T332 |
T330 |
pobj |
context,on |
R128 |
T240 |
T241 |
amod |
single,stranded |
R129 |
T241 |
T233 |
amod |
stranded,segments |
R130 |
T333 |
T332 |
compound |
sequence,context |
R131 |
T242 |
T241 |
punct |
-,stranded |
R132 |
T243 |
T233 |
amod |
adjacent,segments |
R133 |
T334 |
T332 |
prep |
of,context |
R134 |
T244 |
T243 |
prep |
to,adjacent |
R135 |
T245 |
T246 |
det |
the,points |
R136 |
T335 |
T336 |
det |
the,tail |
R137 |
T246 |
T244 |
pobj |
points,to |
R138 |
T247 |
T246 |
compound |
branch,points |
R139 |
T336 |
T334 |
pobj |
tail,of |
R140 |
T248 |
T218 |
punct |
", ",makes |
R141 |
T249 |
T250 |
advmod |
presumably,generating |
R142 |
T337 |
T336 |
nummod |
3,tail |
R143 |
T250 |
T218 |
advcl |
generating,makes |
R144 |
T251 |
T252 |
amod |
double,hexamers |
R145 |
T338 |
T337 |
punct |
′,3 |
R146 |
T252 |
T250 |
dobj |
hexamers,generating |
R147 |
T253 |
T218 |
punct |
.,makes |
R148 |
T339 |
T336 |
punct |
-,tail |
R149 |
T255 |
T256 |
nsubj |
Loss,results |
R150 |
T340 |
T326 |
punct |
", ",depends |
R151 |
T257 |
T255 |
prep |
of,Loss |
R152 |
T258 |
T259 |
compound |
thymine,residues |
R153 |
T259 |
T257 |
pobj |
residues,of |
R154 |
T341 |
T326 |
cc |
and,depends |
R155 |
T342 |
T343 |
det |
the,level |
R156 |
T260 |
T255 |
prep |
from,Loss |
R157 |
T261 |
T262 |
nummod |
one,strand |
R158 |
T262 |
T260 |
pobj |
strand,from |
R159 |
T343 |
T345 |
nsubjpass |
level,achieved |
R160 |
T263 |
T262 |
amod |
single,strand |
R161 |
T344 |
T343 |
amod |
maximum,level |
R162 |
T264 |
T262 |
punct |
-,strand |
R163 |
T265 |
T256 |
prep |
in,results |
R164 |
T345 |
T326 |
conj |
achieved,depends |
R165 |
T266 |
T267 |
amod |
significant,decrease |
R166 |
T267 |
T265 |
pobj |
decrease,in |
R167 |
T346 |
T345 |
auxpass |
is,achieved |
R168 |
T268 |
T267 |
prep |
of,decrease |
R169 |
T269 |
T270 |
amod |
unwinding,efficacy |
R170 |
T270 |
T268 |
pobj |
efficacy,of |
R171 |
T347 |
T345 |
agent |
by,achieved |
R172 |
T271 |
T256 |
punct |
", ",results |
R173 |
T272 |
T256 |
advcl |
suggesting,results |
R174 |
T348 |
T347 |
pobj |
DNA,by |
R175 |
T273 |
T274 |
mark |
that,play |
R176 |
T349 |
T348 |
prep |
with,DNA |
R177 |
T274 |
T272 |
ccomp |
play,suggesting |
R178 |
T350 |
T351 |
nummod |
50,% |
R179 |
T275 |
T276 |
amod |
concurrent,unwinding |
R180 |
T276 |
T274 |
nsubj |
unwinding,play |
R181 |
T351 |
T352 |
nmod |
%,content |
R182 |
T277 |
T276 |
amod |
bidirectional,unwinding |
R183 |
T278 |
T276 |
prep |
by,unwinding |
R184 |
T352 |
T349 |
pobj |
content,with |
R185 |
T279 |
T280 |
det |
a,Mcm4 |
R186 |
T280 |
T278 |
pobj |
Mcm4,by |
R187 |
T281 |
T280 |
amod |
single,Mcm4 |
R188 |
T353 |
T354 |
cc |
or,more |
R189 |
T282 |
T283 |
amod |
double,hexameric |
R190 |
T283 |
T280 |
amod |
hexameric,Mcm4 |
R191 |
T354 |
T351 |
nummod |
more,% |
R192 |
T284 |
T280 |
punct |
/,Mcm4 |
R193 |
T285 |
T280 |
nummod |
6,Mcm4 |
R194 |
T355 |
T352 |
compound |
thymine,content |
R195 |
T286 |
T280 |
punct |
/,Mcm4 |
R196 |
T287 |
T280 |
nummod |
7,Mcm4 |
R197 |
T356 |
T345 |
punct |
.,achieved |
R198 |
T288 |
T274 |
aux |
may,play |
R199 |
T289 |
T290 |
det |
a,role |
R200 |
T358 |
T359 |
compound |
Strand,displacement |
R201 |
T290 |
T274 |
dobj |
role,play |
R202 |
T359 |
T360 |
nsubjpass |
displacement,inhibited |
R203 |
T361 |
T359 |
prep |
by,displacement |
R204 |
T362 |
T361 |
pobj |
Mcm4,by |
R205 |
T363 |
T362 |
punct |
/,Mcm4 |
R206 |
T364 |
T362 |
nummod |
6,Mcm4 |
R207 |
T365 |
T362 |
punct |
/,Mcm4 |
R208 |
T366 |
T362 |
nummod |
7,Mcm4 |
R209 |
T397 |
T381 |
advcl |
suggesting,restored |
R210 |
T398 |
T399 |
mark |
that,has |
R211 |
T399 |
T397 |
ccomp |
has,suggesting |
R212 |
T367 |
T360 |
auxpass |
is,inhibited |
R213 |
T400 |
T401 |
amod |
mammalian,helicase |
R214 |
T401 |
T399 |
nsubj |
helicase,has |
R215 |
T368 |
T360 |
punct |
", ",inhibited |
R216 |
T402 |
T401 |
nmod |
Mcm4,helicase |
R217 |
T403 |
T402 |
punct |
/,Mcm4 |
R218 |
T369 |
T370 |
mark |
as,increases |
R219 |
T404 |
T402 |
nummod |
6,Mcm4 |
R220 |
T405 |
T402 |
punct |
/,Mcm4 |
R221 |
T406 |
T402 |
nummod |
7,Mcm4 |
R222 |
T370 |
T360 |
advcl |
increases,inhibited |
R223 |
T407 |
T399 |
dobj |
difficulties,has |
R224 |
T408 |
T407 |
prep |
in,difficulties |
R225 |
T371 |
T372 |
det |
the,content |
R226 |
T409 |
T408 |
pcomp |
unwinding,in |
R227 |
T372 |
T370 |
nsubj |
content,increases |
R228 |
T410 |
T411 |
advmod |
stably,paired |
R229 |
T411 |
T414 |
amod |
paired,duplex |
R230 |
T412 |
T411 |
npadvmod |
base,paired |
R231 |
T373 |
T372 |
compound |
GC,content |
R232 |
T413 |
T411 |
punct |
-,paired |
R233 |
T414 |
T409 |
dobj |
duplex,unwinding |
R234 |
T374 |
T372 |
prep |
of,content |
R235 |
T375 |
T376 |
det |
the,region |
R236 |
T415 |
T381 |
punct |
.,restored |
R237 |
T376 |
T374 |
pobj |
region,of |
R238 |
T417 |
T418 |
advcl |
Taken,reveal |
R239 |
T377 |
T376 |
compound |
duplex,region |
R240 |
T419 |
T417 |
advmod |
together,Taken |
R241 |
T378 |
T360 |
punct |
.,inhibited |
R242 |
T380 |
T381 |
nsubj |
Replacement,restored |
R243 |
T420 |
T418 |
punct |
", ",reveal |
R244 |
T421 |
T422 |
det |
these,findings |
R245 |
T382 |
T380 |
prep |
of,Replacement |
R246 |
T422 |
T418 |
nsubj |
findings,reveal |
R247 |
T423 |
T424 |
amod |
important,features |
R248 |
T424 |
T418 |
dobj |
features,reveal |
R249 |
T383 |
T384 |
compound |
cytosine,guanine |
R250 |
T425 |
T424 |
prep |
on,features |
R251 |
T384 |
T386 |
compound |
guanine,pairs |
R252 |
T426 |
T427 |
nmod |
activation,preference |
R253 |
T427 |
T425 |
pobj |
preference,on |
R254 |
T428 |
T426 |
cc |
and,activation |
R255 |
T385 |
T384 |
punct |
–,guanine |
R256 |
T429 |
T426 |
conj |
substrate,activation |
R257 |
T430 |
T427 |
prep |
of,preference |
R258 |
T386 |
T382 |
pobj |
pairs,of |
R259 |
T431 |
T432 |
det |
the,helicase |
R260 |
T432 |
T430 |
pobj |
helicase,of |
R261 |
T387 |
T380 |
prep |
with,Replacement |
R262 |
T433 |
T432 |
amod |
eukaryotic,helicase |
R263 |
T434 |
T432 |
amod |
replicative,helicase |
R264 |
T435 |
T418 |
punct |
.,reveal |
R265 |
T388 |
T389 |
compound |
cytosine,inosine |
R266 |
T389 |
T391 |
compound |
inosine,pairs |
R267 |
T390 |
T389 |
punct |
–,inosine |
R268 |
T391 |
T387 |
pobj |
pairs,with |
R269 |
T392 |
T380 |
prep |
in,Replacement |
R270 |
T393 |
T394 |
det |
the,duplex |
R271 |
T394 |
T392 |
pobj |
duplex,in |
R272 |
T395 |
T381 |
dobj |
unwinding,restored |
R273 |
T396 |
T381 |
punct |
", ",restored |