Id |
Subject |
Object |
Predicate |
Lexical cue |
T2043 |
0-3 |
DT |
denotes |
The |
T2044 |
15-25 |
NNS |
denotes |
substrates |
T2045 |
4-7 |
NN |
denotes |
DNA |
T2046 |
8-14 |
NN |
denotes |
bubble |
T2047 |
31-40 |
VBN |
denotes |
assembled |
T2048 |
26-30 |
VBD |
denotes |
were |
T2049 |
41-45 |
IN |
denotes |
from |
T2050 |
46-49 |
CD |
denotes |
two |
T2051 |
74-90 |
NNS |
denotes |
oligonucleotides |
T2052 |
50-59 |
RB |
denotes |
partially |
T2053 |
60-73 |
JJ |
denotes |
complementary |
T2054 |
91-95 |
IN |
denotes |
with |
T2055 |
96-99 |
JJ |
denotes |
top |
T2056 |
118-127 |
NNS |
denotes |
sequences |
T2057 |
100-103 |
CC |
denotes |
and |
T2058 |
104-110 |
JJ |
denotes |
bottom |
T2059 |
111-117 |
NN |
denotes |
strand |
T2060 |
128-129 |
-LRB- |
denotes |
( |
T2061 |
129-134 |
NN |
denotes |
Table |
T2062 |
135-136 |
CD |
denotes |
1 |
T2063 |
136-137 |
-RRB- |
denotes |
) |
T2064 |
137-138 |
. |
denotes |
. |
T2065 |
138-237 |
sentence |
denotes |
T-tailed Y-fork substrates were prepared by annealing two oligonucleotides (dT30 and dT60 series). |
T2066 |
139-140 |
NN |
denotes |
T |
T2067 |
141-147 |
VBN |
denotes |
tailed |
T2068 |
140-141 |
HYPH |
denotes |
- |
T2069 |
155-165 |
NNS |
denotes |
substrates |
T2070 |
148-149 |
NN |
denotes |
Y |
T2071 |
150-154 |
NN |
denotes |
fork |
T2072 |
149-150 |
HYPH |
denotes |
- |
T2073 |
171-179 |
VBN |
denotes |
prepared |
T2074 |
166-170 |
VBD |
denotes |
were |
T2075 |
180-182 |
IN |
denotes |
by |
T2076 |
183-192 |
VBG |
denotes |
annealing |
T2077 |
193-196 |
CD |
denotes |
two |
T2078 |
197-213 |
NNS |
denotes |
oligonucleotides |
T2079 |
214-215 |
-LRB- |
denotes |
( |
T2080 |
229-235 |
NNS |
denotes |
series |
T2081 |
215-219 |
NN |
denotes |
dT30 |
T2082 |
220-223 |
CC |
denotes |
and |
T2083 |
224-228 |
NN |
denotes |
dT60 |
T2084 |
235-236 |
-RRB- |
denotes |
) |
T2085 |
236-237 |
. |
denotes |
. |
T2086 |
237-377 |
sentence |
denotes |
To construct the arrested fork and 3′- or 5′-extension substrates, various combinations of oligonucleotides shown in Table 1 were annealed. |
T2087 |
238-240 |
TO |
denotes |
To |
T2088 |
241-250 |
VB |
denotes |
construct |
T2089 |
368-376 |
VBN |
denotes |
annealed |
T2090 |
251-254 |
DT |
denotes |
the |
T2091 |
264-268 |
NN |
denotes |
fork |
T2092 |
255-263 |
VBN |
denotes |
arrested |
T2093 |
269-272 |
CC |
denotes |
and |
T2094 |
273-274 |
CD |
denotes |
3 |
T2095 |
293-303 |
NNS |
denotes |
substrates |
T2096 |
274-275 |
SYM |
denotes |
′ |
T2097 |
275-276 |
HYPH |
denotes |
- |
T2098 |
277-279 |
CC |
denotes |
or |
T2099 |
280-281 |
CD |
denotes |
5 |
T2100 |
281-282 |
SYM |
denotes |
′ |
T2101 |
282-283 |
HYPH |
denotes |
- |
T2102 |
283-292 |
NN |
denotes |
extension |
T2103 |
303-305 |
, |
denotes |
, |
T2104 |
305-312 |
JJ |
denotes |
various |
T2105 |
313-325 |
NNS |
denotes |
combinations |
T2106 |
326-328 |
IN |
denotes |
of |
T2107 |
329-345 |
NNS |
denotes |
oligonucleotides |
T2108 |
346-351 |
VBN |
denotes |
shown |
T2109 |
352-354 |
IN |
denotes |
in |
T2110 |
355-360 |
NN |
denotes |
Table |
T2111 |
361-362 |
CD |
denotes |
1 |
T2112 |
363-367 |
VBD |
denotes |
were |
T2113 |
376-377 |
. |
denotes |
. |
T2114 |
377-498 |
sentence |
denotes |
Bub-82/lamin B2 containing the T-rich strand of the lamin B2 origin region was constructed as previously described (13). |
T2115 |
378-381 |
NN |
denotes |
Bub |
T2116 |
391-393 |
NN |
denotes |
B2 |
T2117 |
381-382 |
HYPH |
denotes |
- |
T2118 |
382-384 |
CD |
denotes |
82 |
T2119 |
384-385 |
HYPH |
denotes |
/ |
T2120 |
385-390 |
NN |
denotes |
lamin |
T2121 |
457-468 |
VBN |
denotes |
constructed |
T2122 |
394-404 |
VBG |
denotes |
containing |
T2123 |
405-408 |
DT |
denotes |
the |
T2124 |
416-422 |
NN |
denotes |
strand |
T2125 |
409-410 |
NN |
denotes |
T |
T2126 |
411-415 |
JJ |
denotes |
rich |
T2127 |
410-411 |
HYPH |
denotes |
- |
T2128 |
423-425 |
IN |
denotes |
of |
T2129 |
426-429 |
DT |
denotes |
the |
T2130 |
446-452 |
NN |
denotes |
region |
T2131 |
430-435 |
NN |
denotes |
lamin |
T2132 |
436-438 |
NN |
denotes |
B2 |
T2133 |
439-445 |
NN |
denotes |
origin |
T2134 |
453-456 |
VBD |
denotes |
was |
T2135 |
469-471 |
IN |
denotes |
as |
T2136 |
483-492 |
VBN |
denotes |
described |
T2137 |
472-482 |
RB |
denotes |
previously |
T2138 |
493-494 |
-LRB- |
denotes |
( |
T2139 |
494-496 |
CD |
denotes |
13 |
T2140 |
496-497 |
-RRB- |
denotes |
) |
T2141 |
497-498 |
. |
denotes |
. |
T2142 |
498-720 |
sentence |
denotes |
The bubble substrate, c-myc/DUE or c-myc/DUE-C, contains sequences (GenBank accession number K01908) from nucleotides 722 to 853 or those from 828 to 747 (complementary strand), respectively, in the central melted region. |
T2143 |
499-502 |
DT |
denotes |
The |
T2144 |
510-519 |
NN |
denotes |
substrate |
T2145 |
503-509 |
NN |
denotes |
bubble |
T2146 |
547-555 |
VBZ |
denotes |
contains |
T2147 |
519-521 |
, |
denotes |
, |
T2148 |
521-522 |
NN |
denotes |
c |
T2149 |
523-526 |
NN |
denotes |
myc |
T2150 |
522-523 |
HYPH |
denotes |
- |
T2151 |
527-530 |
NN |
denotes |
DUE |
T2152 |
526-527 |
HYPH |
denotes |
/ |
T2153 |
531-533 |
CC |
denotes |
or |
T2154 |
534-535 |
NN |
denotes |
c |
T2155 |
536-539 |
NN |
denotes |
myc |
T2156 |
535-536 |
HYPH |
denotes |
- |
T2157 |
544-545 |
NN |
denotes |
C |
T2158 |
539-540 |
HYPH |
denotes |
/ |
T2159 |
540-543 |
NN |
denotes |
DUE |
T2160 |
543-544 |
HYPH |
denotes |
- |
T2161 |
545-547 |
, |
denotes |
, |
T2162 |
556-565 |
NNS |
denotes |
sequences |
T2163 |
566-567 |
-LRB- |
denotes |
( |
T2164 |
592-598 |
NN |
denotes |
K01908 |
T2165 |
567-574 |
NNP |
denotes |
GenBank |
T2166 |
585-591 |
NN |
denotes |
number |
T2167 |
575-584 |
NN |
denotes |
accession |
T2168 |
598-599 |
-RRB- |
denotes |
) |
T2169 |
600-604 |
IN |
denotes |
from |
T2170 |
605-616 |
NNS |
denotes |
nucleotides |
T2171 |
624-627 |
CD |
denotes |
853 |
T2172 |
617-620 |
CD |
denotes |
722 |
T2173 |
621-623 |
IN |
denotes |
to |
T2174 |
628-630 |
CC |
denotes |
or |
T2175 |
631-636 |
DT |
denotes |
those |
T2176 |
637-641 |
IN |
denotes |
from |
T2177 |
642-645 |
CD |
denotes |
828 |
T2178 |
646-648 |
IN |
denotes |
to |
T2179 |
649-652 |
CD |
denotes |
747 |
T2180 |
653-654 |
-LRB- |
denotes |
( |
T2181 |
668-674 |
NN |
denotes |
strand |
T2182 |
654-667 |
JJ |
denotes |
complementary |
T2183 |
674-675 |
-RRB- |
denotes |
) |
T2184 |
675-677 |
, |
denotes |
, |
T2185 |
677-689 |
RB |
denotes |
respectively |
T2186 |
689-691 |
, |
denotes |
, |
T2187 |
691-693 |
IN |
denotes |
in |
T2188 |
694-697 |
DT |
denotes |
the |
T2189 |
713-719 |
NN |
denotes |
region |
T2190 |
698-705 |
JJ |
denotes |
central |
T2191 |
706-712 |
VBN |
denotes |
melted |
T2192 |
719-720 |
. |
denotes |
. |
T2193 |
720-1019 |
sentence |
denotes |
For each substrate, labeled oligonucleotide (3 pmol) was annealed with the unlabeled oligonucleotide (6 pmol) in a reaction mixture (50 μl) containing 20 mM Tris–HCl (pH 7.5), 10 mM MgCl2 and 25 mM NaCl, which were heated to 95°C, kept at 67°C for 1 h, and then allowed to slowly cool down to 37°C. |
T2194 |
721-724 |
IN |
denotes |
For |
T2195 |
778-786 |
VBN |
denotes |
annealed |
T2196 |
725-729 |
DT |
denotes |
each |
T2197 |
730-739 |
NN |
denotes |
substrate |
T2198 |
739-741 |
, |
denotes |
, |
T2199 |
741-748 |
VBN |
denotes |
labeled |
T2200 |
749-764 |
NN |
denotes |
oligonucleotide |
T2201 |
765-766 |
-LRB- |
denotes |
( |
T2202 |
768-772 |
NN |
denotes |
pmol |
T2203 |
766-767 |
CD |
denotes |
3 |
T2204 |
772-773 |
-RRB- |
denotes |
) |
T2205 |
774-777 |
VBD |
denotes |
was |
T2206 |
787-791 |
IN |
denotes |
with |
T2207 |
792-795 |
DT |
denotes |
the |
T2208 |
806-821 |
NN |
denotes |
oligonucleotide |
T2209 |
796-805 |
JJ |
denotes |
unlabeled |
T2210 |
822-823 |
-LRB- |
denotes |
( |
T2211 |
825-829 |
NN |
denotes |
pmol |
T2212 |
823-824 |
CD |
denotes |
6 |
T2213 |
829-830 |
-RRB- |
denotes |
) |
T2214 |
831-833 |
IN |
denotes |
in |
T2215 |
834-835 |
DT |
denotes |
a |
T2216 |
845-852 |
NN |
denotes |
mixture |
T2217 |
836-844 |
NN |
denotes |
reaction |
T2218 |
853-854 |
-LRB- |
denotes |
( |
T2219 |
857-859 |
NN |
denotes |
μl |
T2220 |
854-856 |
CD |
denotes |
50 |
T2221 |
859-860 |
-RRB- |
denotes |
) |
T2222 |
861-871 |
VBG |
denotes |
containing |
T2223 |
872-874 |
CD |
denotes |
20 |
T2224 |
875-877 |
NN |
denotes |
mM |
T2225 |
883-886 |
NN |
denotes |
HCl |
T2226 |
878-882 |
NN |
denotes |
Tris |
T2227 |
882-883 |
HYPH |
denotes |
– |
T2228 |
887-888 |
-LRB- |
denotes |
( |
T2229 |
888-890 |
NN |
denotes |
pH |
T2230 |
891-894 |
CD |
denotes |
7.5 |
T2231 |
894-895 |
-RRB- |
denotes |
) |
T2232 |
895-897 |
, |
denotes |
, |
T2233 |
897-899 |
CD |
denotes |
10 |
T2234 |
900-902 |
NN |
denotes |
mM |
T2235 |
903-908 |
NN |
denotes |
MgCl2 |
T2236 |
909-912 |
CC |
denotes |
and |
T2237 |
913-915 |
CD |
denotes |
25 |
T2238 |
916-918 |
NN |
denotes |
mM |
T2239 |
919-923 |
NN |
denotes |
NaCl |
T2240 |
923-925 |
, |
denotes |
, |
T2241 |
925-930 |
WDT |
denotes |
which |
T2242 |
936-942 |
VBN |
denotes |
heated |
T2243 |
931-935 |
VBD |
denotes |
were |
T2244 |
943-945 |
IN |
denotes |
to |
T2245 |
946-948 |
CD |
denotes |
95 |
T2246 |
948-950 |
NN |
denotes |
°C |
T2247 |
950-952 |
, |
denotes |
, |
T2248 |
952-956 |
VBD |
denotes |
kept |
T2249 |
957-959 |
IN |
denotes |
at |
T2250 |
960-962 |
CD |
denotes |
67 |
T2251 |
962-964 |
NN |
denotes |
°C |
T2252 |
965-968 |
IN |
denotes |
for |
T2253 |
969-970 |
CD |
denotes |
1 |
T2254 |
971-972 |
NN |
denotes |
h |
T2255 |
972-974 |
, |
denotes |
, |
T2256 |
974-977 |
CC |
denotes |
and |
T2257 |
978-982 |
RB |
denotes |
then |
T2258 |
983-990 |
VBN |
denotes |
allowed |
T2259 |
991-993 |
TO |
denotes |
to |
T2260 |
1001-1005 |
VB |
denotes |
cool |
T2261 |
994-1000 |
RB |
denotes |
slowly |
T2262 |
1006-1010 |
RP |
denotes |
down |
T2263 |
1011-1013 |
IN |
denotes |
to |
T2264 |
1014-1016 |
CD |
denotes |
37 |
T2265 |
1016-1018 |
NN |
denotes |
°C |
T2266 |
1018-1019 |
. |
denotes |
. |
T2267 |
1019-1114 |
sentence |
denotes |
The assembled substrates were purified from polyacrylamide gel by elution into TE buffer (24). |
T2268 |
1020-1023 |
DT |
denotes |
The |
T2269 |
1034-1044 |
NNS |
denotes |
substrates |
T2270 |
1024-1033 |
VBN |
denotes |
assembled |
T2271 |
1050-1058 |
VBN |
denotes |
purified |
T2272 |
1045-1049 |
VBD |
denotes |
were |
T2273 |
1059-1063 |
IN |
denotes |
from |
T2274 |
1064-1078 |
NN |
denotes |
polyacrylamide |
T2275 |
1079-1082 |
NN |
denotes |
gel |
T2276 |
1083-1085 |
IN |
denotes |
by |
T2277 |
1086-1093 |
NN |
denotes |
elution |
T2278 |
1094-1098 |
IN |
denotes |
into |
T2279 |
1099-1101 |
NN |
denotes |
TE |
T2280 |
1102-1108 |
NN |
denotes |
buffer |
T2281 |
1109-1110 |
-LRB- |
denotes |
( |
T2282 |
1110-1112 |
CD |
denotes |
24 |
T2283 |
1112-1113 |
-RRB- |
denotes |
) |
T2284 |
1113-1114 |
. |
denotes |
. |
R1179 |
T2043 |
T2044 |
det |
The,substrates |
R1180 |
T2044 |
T2047 |
nsubjpass |
substrates,assembled |
R1181 |
T2045 |
T2046 |
compound |
DNA,bubble |
R1182 |
T2046 |
T2044 |
compound |
bubble,substrates |
R1183 |
T2048 |
T2047 |
auxpass |
were,assembled |
R1184 |
T2049 |
T2047 |
prep |
from,assembled |
R1185 |
T2050 |
T2051 |
nummod |
two,oligonucleotides |
R1186 |
T2051 |
T2049 |
pobj |
oligonucleotides,from |
R1187 |
T2052 |
T2053 |
advmod |
partially,complementary |
R1188 |
T2053 |
T2051 |
amod |
complementary,oligonucleotides |
R1189 |
T2054 |
T2051 |
prep |
with,oligonucleotides |
R1190 |
T2055 |
T2056 |
amod |
top,sequences |
R1191 |
T2056 |
T2054 |
pobj |
sequences,with |
R1192 |
T2057 |
T2055 |
cc |
and,top |
R1193 |
T2058 |
T2055 |
conj |
bottom,top |
R1194 |
T2059 |
T2056 |
compound |
strand,sequences |
R1195 |
T2060 |
T2061 |
punct |
(,Table |
R1196 |
T2061 |
T2051 |
parataxis |
Table,oligonucleotides |
R1197 |
T2062 |
T2061 |
nummod |
1,Table |
R1198 |
T2063 |
T2061 |
punct |
),Table |
R1199 |
T2064 |
T2047 |
punct |
.,assembled |
R1200 |
T2066 |
T2067 |
npadvmod |
T,tailed |
R1201 |
T2067 |
T2069 |
amod |
tailed,substrates |
R1202 |
T2068 |
T2067 |
punct |
-,tailed |
R1203 |
T2069 |
T2073 |
nsubjpass |
substrates,prepared |
R1204 |
T2070 |
T2071 |
compound |
Y,fork |
R1205 |
T2071 |
T2069 |
compound |
fork,substrates |
R1206 |
T2072 |
T2071 |
punct |
-,fork |
R1207 |
T2074 |
T2073 |
auxpass |
were,prepared |
R1208 |
T2075 |
T2073 |
prep |
by,prepared |
R1209 |
T2076 |
T2075 |
pcomp |
annealing,by |
R1210 |
T2077 |
T2078 |
nummod |
two,oligonucleotides |
R1211 |
T2078 |
T2076 |
dobj |
oligonucleotides,annealing |
R1212 |
T2079 |
T2080 |
punct |
(,series |
R1213 |
T2080 |
T2078 |
parataxis |
series,oligonucleotides |
R1214 |
T2081 |
T2080 |
nmod |
dT30,series |
R1215 |
T2082 |
T2081 |
cc |
and,dT30 |
R1216 |
T2083 |
T2081 |
conj |
dT60,dT30 |
R1217 |
T2084 |
T2080 |
punct |
),series |
R1218 |
T2085 |
T2073 |
punct |
.,prepared |
R1219 |
T2087 |
T2088 |
aux |
To,construct |
R1220 |
T2088 |
T2089 |
advcl |
construct,annealed |
R1221 |
T2090 |
T2091 |
det |
the,fork |
R1222 |
T2091 |
T2088 |
dobj |
fork,construct |
R1223 |
T2092 |
T2091 |
amod |
arrested,fork |
R1224 |
T2093 |
T2091 |
cc |
and,fork |
R1225 |
T2094 |
T2095 |
nummod |
3,substrates |
R1226 |
T2095 |
T2091 |
conj |
substrates,fork |
R1227 |
T2096 |
T2094 |
punct |
′,3 |
R1228 |
T2097 |
T2094 |
punct |
-,3 |
R1229 |
T2098 |
T2094 |
cc |
or,3 |
R1230 |
T2099 |
T2094 |
conj |
5,3 |
R1231 |
T2100 |
T2099 |
punct |
′,5 |
R1232 |
T2101 |
T2095 |
punct |
-,substrates |
R1233 |
T2102 |
T2095 |
compound |
extension,substrates |
R1234 |
T2103 |
T2089 |
punct |
", ",annealed |
R1235 |
T2104 |
T2105 |
amod |
various,combinations |
R1236 |
T2105 |
T2089 |
nsubjpass |
combinations,annealed |
R1237 |
T2106 |
T2105 |
prep |
of,combinations |
R1238 |
T2107 |
T2106 |
pobj |
oligonucleotides,of |
R1239 |
T2108 |
T2107 |
acl |
shown,oligonucleotides |
R1240 |
T2109 |
T2108 |
prep |
in,shown |
R1241 |
T2110 |
T2109 |
pobj |
Table,in |
R1242 |
T2111 |
T2110 |
nummod |
1,Table |
R1243 |
T2112 |
T2089 |
auxpass |
were,annealed |
R1244 |
T2113 |
T2089 |
punct |
.,annealed |
R1245 |
T2115 |
T2116 |
nmod |
Bub,B2 |
R1246 |
T2116 |
T2121 |
nsubjpass |
B2,constructed |
R1247 |
T2117 |
T2116 |
punct |
-,B2 |
R1248 |
T2118 |
T2116 |
nummod |
82,B2 |
R1249 |
T2119 |
T2116 |
punct |
/,B2 |
R1250 |
T2120 |
T2116 |
compound |
lamin,B2 |
R1251 |
T2122 |
T2116 |
acl |
containing,B2 |
R1252 |
T2123 |
T2124 |
det |
the,strand |
R1253 |
T2124 |
T2122 |
dobj |
strand,containing |
R1254 |
T2125 |
T2126 |
npadvmod |
T,rich |
R1255 |
T2126 |
T2124 |
amod |
rich,strand |
R1256 |
T2127 |
T2126 |
punct |
-,rich |
R1257 |
T2128 |
T2124 |
prep |
of,strand |
R1258 |
T2129 |
T2130 |
det |
the,region |
R1259 |
T2130 |
T2128 |
pobj |
region,of |
R1260 |
T2131 |
T2132 |
compound |
lamin,B2 |
R1261 |
T2132 |
T2130 |
compound |
B2,region |
R1262 |
T2133 |
T2130 |
compound |
origin,region |
R1263 |
T2134 |
T2121 |
auxpass |
was,constructed |
R1264 |
T2135 |
T2136 |
mark |
as,described |
R1265 |
T2136 |
T2121 |
advcl |
described,constructed |
R1266 |
T2137 |
T2136 |
advmod |
previously,described |
R1267 |
T2138 |
T2139 |
punct |
(,13 |
R1268 |
T2139 |
T2136 |
parataxis |
13,described |
R1269 |
T2140 |
T2139 |
punct |
),13 |
R1270 |
T2141 |
T2121 |
punct |
.,constructed |
R1271 |
T2143 |
T2144 |
det |
The,substrate |
R1272 |
T2144 |
T2146 |
nsubj |
substrate,contains |
R1273 |
T2145 |
T2144 |
compound |
bubble,substrate |
R1274 |
T2147 |
T2144 |
punct |
", ",substrate |
R1275 |
T2148 |
T2149 |
compound |
c,myc |
R1276 |
T2149 |
T2151 |
compound |
myc,DUE |
R1277 |
T2150 |
T2149 |
punct |
-,myc |
R1278 |
T2151 |
T2144 |
appos |
DUE,substrate |
R1279 |
T2152 |
T2151 |
punct |
/,DUE |
R1280 |
T2153 |
T2151 |
cc |
or,DUE |
R1281 |
T2154 |
T2155 |
compound |
c,myc |
R1282 |
T2155 |
T2157 |
compound |
myc,C |
R1283 |
T2156 |
T2155 |
punct |
-,myc |
R1284 |
T2157 |
T2151 |
conj |
C,DUE |
R1285 |
T2158 |
T2157 |
punct |
/,C |
R1286 |
T2159 |
T2157 |
compound |
DUE,C |
R1287 |
T2160 |
T2157 |
punct |
-,C |
R1288 |
T2161 |
T2146 |
punct |
", ",contains |
R1289 |
T2162 |
T2146 |
dobj |
sequences,contains |
R1290 |
T2163 |
T2164 |
punct |
(,K01908 |
R1291 |
T2164 |
T2162 |
parataxis |
K01908,sequences |
R1292 |
T2165 |
T2166 |
compound |
GenBank,number |
R1293 |
T2166 |
T2164 |
compound |
number,K01908 |
R1294 |
T2167 |
T2166 |
compound |
accession,number |
R1295 |
T2168 |
T2164 |
punct |
),K01908 |
R1296 |
T2169 |
T2162 |
prep |
from,sequences |
R1297 |
T2170 |
T2171 |
nmod |
nucleotides,853 |
R1298 |
T2171 |
T2169 |
pobj |
853,from |
R1299 |
T2172 |
T2171 |
nummod |
722,853 |
R1300 |
T2173 |
T2171 |
nmod |
to,853 |
R1301 |
T2174 |
T2162 |
cc |
or,sequences |
R1302 |
T2175 |
T2162 |
conj |
those,sequences |
R1303 |
T2176 |
T2175 |
prep |
from,those |
R1304 |
T2177 |
T2176 |
pobj |
828,from |
R1305 |
T2178 |
T2176 |
prep |
to,from |
R1306 |
T2179 |
T2178 |
pobj |
747,to |
R1307 |
T2180 |
T2181 |
punct |
(,strand |
R1308 |
T2181 |
T2175 |
parataxis |
strand,those |
R1309 |
T2182 |
T2181 |
amod |
complementary,strand |
R1310 |
T2183 |
T2181 |
punct |
),strand |
R1311 |
T2184 |
T2146 |
punct |
", ",contains |
R1312 |
T2185 |
T2146 |
advmod |
respectively,contains |
R1313 |
T2186 |
T2146 |
punct |
", ",contains |
R1314 |
T2187 |
T2146 |
prep |
in,contains |
R1315 |
T2188 |
T2189 |
det |
the,region |
R1316 |
T2189 |
T2187 |
pobj |
region,in |
R1317 |
T2190 |
T2189 |
amod |
central,region |
R1318 |
T2191 |
T2189 |
amod |
melted,region |
R1319 |
T2192 |
T2146 |
punct |
.,contains |
R1320 |
T2194 |
T2195 |
prep |
For,annealed |
R1321 |
T2196 |
T2197 |
det |
each,substrate |
R1322 |
T2197 |
T2194 |
pobj |
substrate,For |
R1323 |
T2198 |
T2195 |
punct |
", ",annealed |
R1324 |
T2199 |
T2200 |
amod |
labeled,oligonucleotide |
R1325 |
T2200 |
T2195 |
nsubjpass |
oligonucleotide,annealed |
R1326 |
T2201 |
T2202 |
punct |
(,pmol |
R1327 |
T2202 |
T2200 |
parataxis |
pmol,oligonucleotide |
R1328 |
T2203 |
T2202 |
nummod |
3,pmol |
R1329 |
T2204 |
T2202 |
punct |
),pmol |
R1330 |
T2205 |
T2195 |
auxpass |
was,annealed |
R1331 |
T2206 |
T2195 |
prep |
with,annealed |
R1332 |
T2207 |
T2208 |
det |
the,oligonucleotide |
R1333 |
T2208 |
T2206 |
pobj |
oligonucleotide,with |
R1334 |
T2209 |
T2208 |
amod |
unlabeled,oligonucleotide |
R1335 |
T2210 |
T2211 |
punct |
(,pmol |
R1336 |
T2211 |
T2208 |
parataxis |
pmol,oligonucleotide |
R1337 |
T2212 |
T2211 |
nummod |
6,pmol |
R1338 |
T2213 |
T2211 |
punct |
),pmol |
R1339 |
T2214 |
T2195 |
prep |
in,annealed |
R1340 |
T2215 |
T2216 |
det |
a,mixture |
R1341 |
T2216 |
T2214 |
pobj |
mixture,in |
R1342 |
T2217 |
T2216 |
compound |
reaction,mixture |
R1343 |
T2218 |
T2219 |
punct |
(,μl |
R1344 |
T2219 |
T2216 |
parataxis |
μl,mixture |
R1345 |
T2220 |
T2219 |
nummod |
50,μl |
R1346 |
T2221 |
T2219 |
punct |
),μl |
R1347 |
T2222 |
T2216 |
acl |
containing,mixture |
R1348 |
T2223 |
T2224 |
nummod |
20,mM |
R1349 |
T2224 |
T2225 |
compound |
mM,HCl |
R1350 |
T2225 |
T2222 |
dobj |
HCl,containing |
R1351 |
T2226 |
T2225 |
compound |
Tris,HCl |
R1352 |
T2227 |
T2225 |
punct |
–,HCl |
R1353 |
T2228 |
T2229 |
punct |
(,pH |
R1354 |
T2229 |
T2225 |
parataxis |
pH,HCl |
R1355 |
T2230 |
T2229 |
nummod |
7.5,pH |
R1356 |
T2231 |
T2229 |
punct |
),pH |
R1357 |
T2232 |
T2225 |
punct |
", ",HCl |
R1358 |
T2233 |
T2234 |
nummod |
10,mM |
R1359 |
T2234 |
T2235 |
compound |
mM,MgCl2 |
R1360 |
T2235 |
T2225 |
conj |
MgCl2,HCl |
R1361 |
T2236 |
T2235 |
cc |
and,MgCl2 |
R1362 |
T2237 |
T2238 |
nummod |
25,mM |
R1363 |
T2238 |
T2239 |
compound |
mM,NaCl |
R1364 |
T2239 |
T2235 |
conj |
NaCl,MgCl2 |
R1365 |
T2240 |
T2216 |
punct |
", ",mixture |
R1366 |
T2241 |
T2242 |
dep |
which,heated |
R1367 |
T2242 |
T2216 |
relcl |
heated,mixture |
R1368 |
T2243 |
T2242 |
auxpass |
were,heated |
R1369 |
T2244 |
T2242 |
prep |
to,heated |
R1370 |
T2245 |
T2246 |
nummod |
95,°C |
R1371 |
T2246 |
T2244 |
pobj |
°C,to |
R1372 |
T2247 |
T2242 |
punct |
", ",heated |
R1373 |
T2248 |
T2242 |
conj |
kept,heated |
R1374 |
T2249 |
T2248 |
prep |
at,kept |
R1375 |
T2250 |
T2251 |
nummod |
67,°C |
R1376 |
T2251 |
T2249 |
pobj |
°C,at |
R1377 |
T2252 |
T2248 |
prep |
for,kept |
R1378 |
T2253 |
T2254 |
nummod |
1,h |
R1379 |
T2254 |
T2252 |
pobj |
h,for |
R1380 |
T2255 |
T2248 |
punct |
", ",kept |
R1381 |
T2256 |
T2248 |
cc |
and,kept |
R1382 |
T2257 |
T2258 |
advmod |
then,allowed |
R1383 |
T2258 |
T2248 |
conj |
allowed,kept |
R1384 |
T2259 |
T2260 |
aux |
to,cool |
R1385 |
T2260 |
T2258 |
xcomp |
cool,allowed |
R1386 |
T2261 |
T2260 |
advmod |
slowly,cool |
R1387 |
T2262 |
T2260 |
prt |
down,cool |
R1388 |
T2263 |
T2260 |
prep |
to,cool |
R1389 |
T2264 |
T2265 |
nummod |
37,°C |
R1390 |
T2265 |
T2263 |
pobj |
°C,to |
R1391 |
T2266 |
T2195 |
punct |
.,annealed |
R1392 |
T2268 |
T2269 |
det |
The,substrates |
R1393 |
T2269 |
T2271 |
nsubjpass |
substrates,purified |
R1394 |
T2270 |
T2269 |
amod |
assembled,substrates |
R1395 |
T2272 |
T2271 |
auxpass |
were,purified |
R1396 |
T2273 |
T2271 |
prep |
from,purified |
R1397 |
T2274 |
T2275 |
compound |
polyacrylamide,gel |
R1398 |
T2275 |
T2273 |
pobj |
gel,from |
R1399 |
T2276 |
T2271 |
prep |
by,purified |
R1400 |
T2277 |
T2276 |
pobj |
elution,by |
R1401 |
T2278 |
T2277 |
prep |
into,elution |
R1402 |
T2279 |
T2280 |
compound |
TE,buffer |
R1403 |
T2280 |
T2278 |
pobj |
buffer,into |
R1404 |
T2281 |
T2282 |
punct |
(,24 |
R1405 |
T2282 |
T2271 |
parataxis |
24,purified |
R1406 |
T2283 |
T2282 |
punct |
),24 |
R1407 |
T2284 |
T2271 |
punct |
.,purified |