Id |
Subject |
Object |
Predicate |
Lexical cue |
T1980 |
0-3 |
NN |
denotes |
DNA |
T1981 |
4-14 |
NNS |
denotes |
substrates |
T1982 |
14-128 |
sentence |
denotes |
The sequences for all the oligonucleotides used for constructions of DNA substrates are listed in Tables 1 and 2. |
T1983 |
15-18 |
DT |
denotes |
The |
T1984 |
19-28 |
NNS |
denotes |
sequences |
T1985 |
103-109 |
VBN |
denotes |
listed |
T1986 |
29-32 |
IN |
denotes |
for |
T1987 |
33-36 |
PDT |
denotes |
all |
T1988 |
41-57 |
NNS |
denotes |
oligonucleotides |
T1989 |
37-40 |
DT |
denotes |
the |
T1990 |
58-62 |
VBN |
denotes |
used |
T1991 |
63-66 |
IN |
denotes |
for |
T1992 |
67-80 |
NNS |
denotes |
constructions |
T1993 |
81-83 |
IN |
denotes |
of |
T1994 |
84-87 |
NN |
denotes |
DNA |
T1995 |
88-98 |
NNS |
denotes |
substrates |
T1996 |
99-102 |
VBP |
denotes |
are |
T1997 |
110-112 |
IN |
denotes |
in |
T1998 |
113-119 |
NNS |
denotes |
Tables |
T1999 |
120-121 |
CD |
denotes |
1 |
T2000 |
122-125 |
CC |
denotes |
and |
T2001 |
126-127 |
CD |
denotes |
2 |
T2002 |
127-128 |
. |
denotes |
. |
T2003 |
128-280 |
sentence |
denotes |
The partial heteroduplex substrates were constructed by annealing labeled oligonucleotides with M13mp18 single-stranded circular DNA or its derivative. |
T2004 |
129-132 |
DT |
denotes |
The |
T2005 |
154-164 |
NNS |
denotes |
substrates |
T2006 |
133-140 |
JJ |
denotes |
partial |
T2007 |
141-153 |
NN |
denotes |
heteroduplex |
T2008 |
170-181 |
VBN |
denotes |
constructed |
T2009 |
165-169 |
VBD |
denotes |
were |
T2010 |
182-184 |
IN |
denotes |
by |
T2011 |
185-194 |
VBG |
denotes |
annealing |
T2012 |
195-202 |
VBN |
denotes |
labeled |
T2013 |
203-219 |
NNS |
denotes |
oligonucleotides |
T2014 |
220-224 |
IN |
denotes |
with |
T2015 |
225-232 |
NN |
denotes |
M13mp18 |
T2016 |
258-261 |
NN |
denotes |
DNA |
T2017 |
233-239 |
JJ |
denotes |
single |
T2018 |
240-248 |
VBN |
denotes |
stranded |
T2019 |
239-240 |
HYPH |
denotes |
- |
T2020 |
249-257 |
JJ |
denotes |
circular |
T2021 |
262-264 |
CC |
denotes |
or |
T2022 |
265-268 |
PRP$ |
denotes |
its |
T2023 |
269-279 |
NN |
denotes |
derivative |
T2024 |
279-280 |
. |
denotes |
. |
T2025 |
280-387 |
sentence |
denotes |
The labeled substrates were purified by Sepharose CL4B column chromatography (Amersham Pharmacia Biotech). |
T2026 |
281-284 |
DT |
denotes |
The |
T2027 |
293-303 |
NNS |
denotes |
substrates |
T2028 |
285-292 |
VBN |
denotes |
labeled |
T2029 |
309-317 |
VBN |
denotes |
purified |
T2030 |
304-308 |
VBD |
denotes |
were |
T2031 |
318-320 |
IN |
denotes |
by |
T2032 |
321-330 |
NN |
denotes |
Sepharose |
T2033 |
331-335 |
NN |
denotes |
CL4B |
T2034 |
343-357 |
NN |
denotes |
chromatography |
T2035 |
336-342 |
NN |
denotes |
column |
T2036 |
358-359 |
-LRB- |
denotes |
( |
T2037 |
378-385 |
NNP |
denotes |
Biotech |
T2038 |
359-367 |
NNP |
denotes |
Amersham |
T2039 |
368-377 |
NNP |
denotes |
Pharmacia |
T2040 |
385-386 |
-RRB- |
denotes |
) |
T2041 |
386-387 |
. |
denotes |
. |
T2042 |
387-526 |
sentence |
denotes |
The DNA bubble substrates were assembled from two partially complementary oligonucleotides with top and bottom strand sequences (Table 1). |
T2043 |
388-391 |
DT |
denotes |
The |
T2044 |
403-413 |
NNS |
denotes |
substrates |
T2045 |
392-395 |
NN |
denotes |
DNA |
T2046 |
396-402 |
NN |
denotes |
bubble |
T2047 |
419-428 |
VBN |
denotes |
assembled |
T2048 |
414-418 |
VBD |
denotes |
were |
T2049 |
429-433 |
IN |
denotes |
from |
T2050 |
434-437 |
CD |
denotes |
two |
T2051 |
462-478 |
NNS |
denotes |
oligonucleotides |
T2052 |
438-447 |
RB |
denotes |
partially |
T2053 |
448-461 |
JJ |
denotes |
complementary |
T2054 |
479-483 |
IN |
denotes |
with |
T2055 |
484-487 |
JJ |
denotes |
top |
T2056 |
506-515 |
NNS |
denotes |
sequences |
T2057 |
488-491 |
CC |
denotes |
and |
T2058 |
492-498 |
JJ |
denotes |
bottom |
T2059 |
499-505 |
NN |
denotes |
strand |
T2060 |
516-517 |
-LRB- |
denotes |
( |
T2061 |
517-522 |
NN |
denotes |
Table |
T2062 |
523-524 |
CD |
denotes |
1 |
T2063 |
524-525 |
-RRB- |
denotes |
) |
T2064 |
525-526 |
. |
denotes |
. |
T2065 |
526-625 |
sentence |
denotes |
T-tailed Y-fork substrates were prepared by annealing two oligonucleotides (dT30 and dT60 series). |
T2066 |
527-528 |
NN |
denotes |
T |
T2067 |
529-535 |
VBN |
denotes |
tailed |
T2068 |
528-529 |
HYPH |
denotes |
- |
T2069 |
543-553 |
NNS |
denotes |
substrates |
T2070 |
536-537 |
NN |
denotes |
Y |
T2071 |
538-542 |
NN |
denotes |
fork |
T2072 |
537-538 |
HYPH |
denotes |
- |
T2073 |
559-567 |
VBN |
denotes |
prepared |
T2074 |
554-558 |
VBD |
denotes |
were |
T2075 |
568-570 |
IN |
denotes |
by |
T2076 |
571-580 |
VBG |
denotes |
annealing |
T2077 |
581-584 |
CD |
denotes |
two |
T2078 |
585-601 |
NNS |
denotes |
oligonucleotides |
T2079 |
602-603 |
-LRB- |
denotes |
( |
T2080 |
617-623 |
NNS |
denotes |
series |
T2081 |
603-607 |
NN |
denotes |
dT30 |
T2082 |
608-611 |
CC |
denotes |
and |
T2083 |
612-616 |
NN |
denotes |
dT60 |
T2084 |
623-624 |
-RRB- |
denotes |
) |
T2085 |
624-625 |
. |
denotes |
. |
T2086 |
625-765 |
sentence |
denotes |
To construct the arrested fork and 3′- or 5′-extension substrates, various combinations of oligonucleotides shown in Table 1 were annealed. |
T2087 |
626-628 |
TO |
denotes |
To |
T2088 |
629-638 |
VB |
denotes |
construct |
T2089 |
756-764 |
VBN |
denotes |
annealed |
T2090 |
639-642 |
DT |
denotes |
the |
T2091 |
652-656 |
NN |
denotes |
fork |
T2092 |
643-651 |
VBN |
denotes |
arrested |
T2093 |
657-660 |
CC |
denotes |
and |
T2094 |
661-662 |
CD |
denotes |
3 |
T2095 |
681-691 |
NNS |
denotes |
substrates |
T2096 |
662-663 |
SYM |
denotes |
′ |
T2097 |
663-664 |
HYPH |
denotes |
- |
T2098 |
665-667 |
CC |
denotes |
or |
T2099 |
668-669 |
CD |
denotes |
5 |
T2100 |
669-670 |
SYM |
denotes |
′ |
T2101 |
670-671 |
HYPH |
denotes |
- |
T2102 |
671-680 |
NN |
denotes |
extension |
T2103 |
691-693 |
, |
denotes |
, |
T2104 |
693-700 |
JJ |
denotes |
various |
T2105 |
701-713 |
NNS |
denotes |
combinations |
T2106 |
714-716 |
IN |
denotes |
of |
T2107 |
717-733 |
NNS |
denotes |
oligonucleotides |
T2108 |
734-739 |
VBN |
denotes |
shown |
T2109 |
740-742 |
IN |
denotes |
in |
T2110 |
743-748 |
NN |
denotes |
Table |
T2111 |
749-750 |
CD |
denotes |
1 |
T2112 |
751-755 |
VBD |
denotes |
were |
T2113 |
764-765 |
. |
denotes |
. |
T2114 |
765-886 |
sentence |
denotes |
Bub-82/lamin B2 containing the T-rich strand of the lamin B2 origin region was constructed as previously described (13). |
T2115 |
766-769 |
NN |
denotes |
Bub |
T2116 |
779-781 |
NN |
denotes |
B2 |
T2117 |
769-770 |
HYPH |
denotes |
- |
T2118 |
770-772 |
CD |
denotes |
82 |
T2119 |
772-773 |
HYPH |
denotes |
/ |
T2120 |
773-778 |
NN |
denotes |
lamin |
T2121 |
845-856 |
VBN |
denotes |
constructed |
T2122 |
782-792 |
VBG |
denotes |
containing |
T2123 |
793-796 |
DT |
denotes |
the |
T2124 |
804-810 |
NN |
denotes |
strand |
T2125 |
797-798 |
NN |
denotes |
T |
T2126 |
799-803 |
JJ |
denotes |
rich |
T2127 |
798-799 |
HYPH |
denotes |
- |
T2128 |
811-813 |
IN |
denotes |
of |
T2129 |
814-817 |
DT |
denotes |
the |
T2130 |
834-840 |
NN |
denotes |
region |
T2131 |
818-823 |
NN |
denotes |
lamin |
T2132 |
824-826 |
NN |
denotes |
B2 |
T2133 |
827-833 |
NN |
denotes |
origin |
T2134 |
841-844 |
VBD |
denotes |
was |
T2135 |
857-859 |
IN |
denotes |
as |
T2136 |
871-880 |
VBN |
denotes |
described |
T2137 |
860-870 |
RB |
denotes |
previously |
T2138 |
881-882 |
-LRB- |
denotes |
( |
T2139 |
882-884 |
CD |
denotes |
13 |
T2140 |
884-885 |
-RRB- |
denotes |
) |
T2141 |
885-886 |
. |
denotes |
. |
T2142 |
886-1108 |
sentence |
denotes |
The bubble substrate, c-myc/DUE or c-myc/DUE-C, contains sequences (GenBank accession number K01908) from nucleotides 722 to 853 or those from 828 to 747 (complementary strand), respectively, in the central melted region. |
T2143 |
887-890 |
DT |
denotes |
The |
T2144 |
898-907 |
NN |
denotes |
substrate |
T2145 |
891-897 |
NN |
denotes |
bubble |
T2146 |
935-943 |
VBZ |
denotes |
contains |
T2147 |
907-909 |
, |
denotes |
, |
T2148 |
909-910 |
NN |
denotes |
c |
T2149 |
911-914 |
NN |
denotes |
myc |
T2150 |
910-911 |
HYPH |
denotes |
- |
T2151 |
915-918 |
NN |
denotes |
DUE |
T2152 |
914-915 |
HYPH |
denotes |
/ |
T2153 |
919-921 |
CC |
denotes |
or |
T2154 |
922-923 |
NN |
denotes |
c |
T2155 |
924-927 |
NN |
denotes |
myc |
T2156 |
923-924 |
HYPH |
denotes |
- |
T2157 |
932-933 |
NN |
denotes |
C |
T2158 |
927-928 |
HYPH |
denotes |
/ |
T2159 |
928-931 |
NN |
denotes |
DUE |
T2160 |
931-932 |
HYPH |
denotes |
- |
T2161 |
933-935 |
, |
denotes |
, |
T2162 |
944-953 |
NNS |
denotes |
sequences |
T2163 |
954-955 |
-LRB- |
denotes |
( |
T2164 |
980-986 |
NN |
denotes |
K01908 |
T2165 |
955-962 |
NNP |
denotes |
GenBank |
T2166 |
973-979 |
NN |
denotes |
number |
T2167 |
963-972 |
NN |
denotes |
accession |
T2168 |
986-987 |
-RRB- |
denotes |
) |
T2169 |
988-992 |
IN |
denotes |
from |
T2170 |
993-1004 |
NNS |
denotes |
nucleotides |
T2171 |
1012-1015 |
CD |
denotes |
853 |
T2172 |
1005-1008 |
CD |
denotes |
722 |
T2173 |
1009-1011 |
IN |
denotes |
to |
T2174 |
1016-1018 |
CC |
denotes |
or |
T2175 |
1019-1024 |
DT |
denotes |
those |
T2176 |
1025-1029 |
IN |
denotes |
from |
T2177 |
1030-1033 |
CD |
denotes |
828 |
T2178 |
1034-1036 |
IN |
denotes |
to |
T2179 |
1037-1040 |
CD |
denotes |
747 |
T2180 |
1041-1042 |
-LRB- |
denotes |
( |
T2181 |
1056-1062 |
NN |
denotes |
strand |
T2182 |
1042-1055 |
JJ |
denotes |
complementary |
T2183 |
1062-1063 |
-RRB- |
denotes |
) |
T2184 |
1063-1065 |
, |
denotes |
, |
T2185 |
1065-1077 |
RB |
denotes |
respectively |
T2186 |
1077-1079 |
, |
denotes |
, |
T2187 |
1079-1081 |
IN |
denotes |
in |
T2188 |
1082-1085 |
DT |
denotes |
the |
T2189 |
1101-1107 |
NN |
denotes |
region |
T2190 |
1086-1093 |
JJ |
denotes |
central |
T2191 |
1094-1100 |
VBN |
denotes |
melted |
T2192 |
1107-1108 |
. |
denotes |
. |
T2193 |
1108-1407 |
sentence |
denotes |
For each substrate, labeled oligonucleotide (3 pmol) was annealed with the unlabeled oligonucleotide (6 pmol) in a reaction mixture (50 μl) containing 20 mM Tris–HCl (pH 7.5), 10 mM MgCl2 and 25 mM NaCl, which were heated to 95°C, kept at 67°C for 1 h, and then allowed to slowly cool down to 37°C. |
T2194 |
1109-1112 |
IN |
denotes |
For |
T2195 |
1166-1174 |
VBN |
denotes |
annealed |
T2196 |
1113-1117 |
DT |
denotes |
each |
T2197 |
1118-1127 |
NN |
denotes |
substrate |
T2198 |
1127-1129 |
, |
denotes |
, |
T2199 |
1129-1136 |
VBN |
denotes |
labeled |
T2200 |
1137-1152 |
NN |
denotes |
oligonucleotide |
T2201 |
1153-1154 |
-LRB- |
denotes |
( |
T2202 |
1156-1160 |
NN |
denotes |
pmol |
T2203 |
1154-1155 |
CD |
denotes |
3 |
T2204 |
1160-1161 |
-RRB- |
denotes |
) |
T2205 |
1162-1165 |
VBD |
denotes |
was |
T2206 |
1175-1179 |
IN |
denotes |
with |
T2207 |
1180-1183 |
DT |
denotes |
the |
T2208 |
1194-1209 |
NN |
denotes |
oligonucleotide |
T2209 |
1184-1193 |
JJ |
denotes |
unlabeled |
T2210 |
1210-1211 |
-LRB- |
denotes |
( |
T2211 |
1213-1217 |
NN |
denotes |
pmol |
T2212 |
1211-1212 |
CD |
denotes |
6 |
T2213 |
1217-1218 |
-RRB- |
denotes |
) |
T2214 |
1219-1221 |
IN |
denotes |
in |
T2215 |
1222-1223 |
DT |
denotes |
a |
T2216 |
1233-1240 |
NN |
denotes |
mixture |
T2217 |
1224-1232 |
NN |
denotes |
reaction |
T2218 |
1241-1242 |
-LRB- |
denotes |
( |
T2219 |
1245-1247 |
NN |
denotes |
μl |
T2220 |
1242-1244 |
CD |
denotes |
50 |
T2221 |
1247-1248 |
-RRB- |
denotes |
) |
T2222 |
1249-1259 |
VBG |
denotes |
containing |
T2223 |
1260-1262 |
CD |
denotes |
20 |
T2224 |
1263-1265 |
NN |
denotes |
mM |
T2225 |
1271-1274 |
NN |
denotes |
HCl |
T2226 |
1266-1270 |
NN |
denotes |
Tris |
T2227 |
1270-1271 |
HYPH |
denotes |
– |
T2228 |
1275-1276 |
-LRB- |
denotes |
( |
T2229 |
1276-1278 |
NN |
denotes |
pH |
T2230 |
1279-1282 |
CD |
denotes |
7.5 |
T2231 |
1282-1283 |
-RRB- |
denotes |
) |
T2232 |
1283-1285 |
, |
denotes |
, |
T2233 |
1285-1287 |
CD |
denotes |
10 |
T2234 |
1288-1290 |
NN |
denotes |
mM |
T2235 |
1291-1296 |
NN |
denotes |
MgCl2 |
T2236 |
1297-1300 |
CC |
denotes |
and |
T2237 |
1301-1303 |
CD |
denotes |
25 |
T2238 |
1304-1306 |
NN |
denotes |
mM |
T2239 |
1307-1311 |
NN |
denotes |
NaCl |
T2240 |
1311-1313 |
, |
denotes |
, |
T2241 |
1313-1318 |
WDT |
denotes |
which |
T2242 |
1324-1330 |
VBN |
denotes |
heated |
T2243 |
1319-1323 |
VBD |
denotes |
were |
T2244 |
1331-1333 |
IN |
denotes |
to |
T2245 |
1334-1336 |
CD |
denotes |
95 |
T2246 |
1336-1338 |
NN |
denotes |
°C |
T2247 |
1338-1340 |
, |
denotes |
, |
T2248 |
1340-1344 |
VBD |
denotes |
kept |
T2249 |
1345-1347 |
IN |
denotes |
at |
T2250 |
1348-1350 |
CD |
denotes |
67 |
T2251 |
1350-1352 |
NN |
denotes |
°C |
T2252 |
1353-1356 |
IN |
denotes |
for |
T2253 |
1357-1358 |
CD |
denotes |
1 |
T2254 |
1359-1360 |
NN |
denotes |
h |
T2255 |
1360-1362 |
, |
denotes |
, |
T2256 |
1362-1365 |
CC |
denotes |
and |
T2257 |
1366-1370 |
RB |
denotes |
then |
T2258 |
1371-1378 |
VBN |
denotes |
allowed |
T2259 |
1379-1381 |
TO |
denotes |
to |
T2260 |
1389-1393 |
VB |
denotes |
cool |
T2261 |
1382-1388 |
RB |
denotes |
slowly |
T2262 |
1394-1398 |
RP |
denotes |
down |
T2263 |
1399-1401 |
IN |
denotes |
to |
T2264 |
1402-1404 |
CD |
denotes |
37 |
T2265 |
1404-1406 |
NN |
denotes |
°C |
T2266 |
1406-1407 |
. |
denotes |
. |
T2267 |
1407-1502 |
sentence |
denotes |
The assembled substrates were purified from polyacrylamide gel by elution into TE buffer (24). |
T2268 |
1408-1411 |
DT |
denotes |
The |
T2269 |
1422-1432 |
NNS |
denotes |
substrates |
T2270 |
1412-1421 |
VBN |
denotes |
assembled |
T2271 |
1438-1446 |
VBN |
denotes |
purified |
T2272 |
1433-1437 |
VBD |
denotes |
were |
T2273 |
1447-1451 |
IN |
denotes |
from |
T2274 |
1452-1466 |
NN |
denotes |
polyacrylamide |
T2275 |
1467-1470 |
NN |
denotes |
gel |
T2276 |
1471-1473 |
IN |
denotes |
by |
T2277 |
1474-1481 |
NN |
denotes |
elution |
T2278 |
1482-1486 |
IN |
denotes |
into |
T2279 |
1487-1489 |
NN |
denotes |
TE |
T2280 |
1490-1496 |
NN |
denotes |
buffer |
T2281 |
1497-1498 |
-LRB- |
denotes |
( |
T2282 |
1498-1500 |
CD |
denotes |
24 |
T2283 |
1500-1501 |
-RRB- |
denotes |
) |
T2284 |
1501-1502 |
. |
denotes |
. |
T2285 |
1502-1796 |
sentence |
denotes |
The partial heteroduplex substrates on a single-stranded circular DNA with varied lengths of duplex regions were prepared by extending DNA chains from the 3′ end of the dT40-37mer (37mer region hybridizing at positions 6289–6326 of M13mp18 vector) annealed to the single-stranded circular DNA. |
T2286 |
1503-1506 |
DT |
denotes |
The |
T2287 |
1528-1538 |
NNS |
denotes |
substrates |
T2288 |
1507-1514 |
JJ |
denotes |
partial |
T2289 |
1515-1527 |
NN |
denotes |
heteroduplex |
T2290 |
1616-1624 |
VBN |
denotes |
prepared |
T2291 |
1539-1541 |
IN |
denotes |
on |
T2292 |
1542-1543 |
DT |
denotes |
a |
T2293 |
1569-1572 |
NN |
denotes |
DNA |
T2294 |
1544-1550 |
JJ |
denotes |
single |
T2295 |
1551-1559 |
VBN |
denotes |
stranded |
T2296 |
1550-1551 |
HYPH |
denotes |
- |
T2297 |
1560-1568 |
JJ |
denotes |
circular |
T2298 |
1573-1577 |
IN |
denotes |
with |
T2299 |
1578-1584 |
VBN |
denotes |
varied |
T2300 |
1585-1592 |
NNS |
denotes |
lengths |
T2301 |
1593-1595 |
IN |
denotes |
of |
T2302 |
1596-1602 |
NN |
denotes |
duplex |
T2303 |
1603-1610 |
NNS |
denotes |
regions |
T2304 |
1611-1615 |
VBD |
denotes |
were |
T2305 |
1625-1627 |
IN |
denotes |
by |
T2306 |
1628-1637 |
VBG |
denotes |
extending |
T2307 |
1638-1641 |
NN |
denotes |
DNA |
T2308 |
1642-1648 |
NNS |
denotes |
chains |
T2309 |
1649-1653 |
IN |
denotes |
from |
T2310 |
1654-1657 |
DT |
denotes |
the |
T2311 |
1661-1664 |
NN |
denotes |
end |
T2312 |
1658-1659 |
CD |
denotes |
3 |
T2313 |
1659-1660 |
SYM |
denotes |
′ |
T2314 |
1665-1667 |
IN |
denotes |
of |
T2315 |
1668-1671 |
DT |
denotes |
the |
T2316 |
1677-1682 |
NN |
denotes |
37mer |
T2317 |
1672-1676 |
NN |
denotes |
dT40 |
T2318 |
1676-1677 |
HYPH |
denotes |
- |
T2319 |
1683-1684 |
-LRB- |
denotes |
( |
T2320 |
1684-1689 |
NN |
denotes |
37mer |
T2321 |
1690-1696 |
NN |
denotes |
region |
T2322 |
1697-1708 |
VBG |
denotes |
hybridizing |
T2323 |
1709-1711 |
IN |
denotes |
at |
T2324 |
1712-1721 |
NNS |
denotes |
positions |
T2325 |
1722-1726 |
CD |
denotes |
6289 |
T2326 |
1726-1727 |
SYM |
denotes |
– |
T2327 |
1727-1731 |
CD |
denotes |
6326 |
T2328 |
1732-1734 |
IN |
denotes |
of |
T2329 |
1735-1742 |
NN |
denotes |
M13mp18 |
T2330 |
1743-1749 |
NN |
denotes |
vector |
T2331 |
1749-1750 |
-RRB- |
denotes |
) |
T2332 |
1751-1759 |
VBN |
denotes |
annealed |
T2333 |
1760-1762 |
IN |
denotes |
to |
T2334 |
1763-1766 |
DT |
denotes |
the |
T2335 |
1792-1795 |
NN |
denotes |
DNA |
T2336 |
1767-1773 |
JJ |
denotes |
single |
T2337 |
1774-1782 |
VBN |
denotes |
stranded |
T2338 |
1773-1774 |
HYPH |
denotes |
- |
T2339 |
1783-1791 |
JJ |
denotes |
circular |
T2340 |
1795-1796 |
. |
denotes |
. |
T2341 |
1796-2017 |
sentence |
denotes |
DNA chains were elongated with Sequenase in the presence of [α-32P]dGTP, followed by extension after addition of ddGTP and all four dNTPs, resulting in substrates containing labeled duplex regions of varied lengths (13). |
T2342 |
1797-1800 |
NN |
denotes |
DNA |
T2343 |
1801-1807 |
NNS |
denotes |
chains |
T2344 |
1813-1822 |
VBN |
denotes |
elongated |
T2345 |
1808-1812 |
VBD |
denotes |
were |
T2346 |
1823-1827 |
IN |
denotes |
with |
T2347 |
1828-1837 |
NN |
denotes |
Sequenase |
T2348 |
1838-1840 |
IN |
denotes |
in |
T2349 |
1841-1844 |
DT |
denotes |
the |
T2350 |
1845-1853 |
NN |
denotes |
presence |
T2351 |
1854-1856 |
IN |
denotes |
of |
T2352 |
1857-1858 |
-LRB- |
denotes |
[ |
T2353 |
1864-1868 |
NN |
denotes |
dGTP |
T2354 |
1858-1859 |
NN |
denotes |
α |
T2355 |
1860-1863 |
NN |
denotes |
32P |
T2356 |
1859-1860 |
HYPH |
denotes |
- |
T2357 |
1863-1864 |
-RRB- |
denotes |
] |
T2358 |
1868-1870 |
, |
denotes |
, |
T2359 |
1870-1878 |
VBN |
denotes |
followed |
T2360 |
1879-1881 |
IN |
denotes |
by |
T2361 |
1882-1891 |
NN |
denotes |
extension |
T2362 |
1892-1897 |
IN |
denotes |
after |
T2363 |
1898-1906 |
NN |
denotes |
addition |
T2364 |
1907-1909 |
IN |
denotes |
of |
T2365 |
1910-1915 |
NN |
denotes |
ddGTP |
T2366 |
1916-1919 |
CC |
denotes |
and |
T2367 |
1920-1923 |
DT |
denotes |
all |
T2368 |
1929-1934 |
NNS |
denotes |
dNTPs |
T2369 |
1924-1928 |
CD |
denotes |
four |
T2370 |
1934-1936 |
, |
denotes |
, |
T2371 |
1936-1945 |
VBG |
denotes |
resulting |
T2372 |
1946-1948 |
IN |
denotes |
in |
T2373 |
1949-1959 |
NNS |
denotes |
substrates |
T2374 |
1960-1970 |
VBG |
denotes |
containing |
T2375 |
1971-1978 |
VBN |
denotes |
labeled |
T2376 |
1986-1993 |
NNS |
denotes |
regions |
T2377 |
1979-1985 |
NN |
denotes |
duplex |
T2378 |
1994-1996 |
IN |
denotes |
of |
T2379 |
1997-2003 |
VBN |
denotes |
varied |
T2380 |
2004-2011 |
NNS |
denotes |
lengths |
T2381 |
2012-2013 |
-LRB- |
denotes |
( |
T2382 |
2013-2015 |
CD |
denotes |
13 |
T2383 |
2015-2016 |
-RRB- |
denotes |
) |
T2384 |
2016-2017 |
. |
denotes |
. |
T2385 |
2017-2291 |
sentence |
denotes |
The substrate (5 fmol) was first incubated with indicated amount of the Mcm4/6/7 complex at 37°C for 1 h in reaction mixture as described (11), and then reactions were stopped by addition of EDTA (20 mM), SDS (0.1%) and 2 μg proteinase K, followed by incubation for 20 min. |
T2386 |
2018-2021 |
DT |
denotes |
The |
T2387 |
2022-2031 |
NN |
denotes |
substrate |
T2388 |
2051-2060 |
VBN |
denotes |
incubated |
T2389 |
2032-2033 |
-LRB- |
denotes |
( |
T2390 |
2035-2039 |
NN |
denotes |
fmol |
T2391 |
2033-2034 |
CD |
denotes |
5 |
T2392 |
2039-2040 |
-RRB- |
denotes |
) |
T2393 |
2041-2044 |
VBD |
denotes |
was |
T2394 |
2045-2050 |
RB |
denotes |
first |
T2395 |
2061-2065 |
IN |
denotes |
with |
T2396 |
2066-2075 |
VBN |
denotes |
indicated |
T2397 |
2076-2082 |
NN |
denotes |
amount |
T2398 |
2083-2085 |
IN |
denotes |
of |
T2399 |
2086-2089 |
DT |
denotes |
the |
T2400 |
2099-2106 |
NN |
denotes |
complex |
T2401 |
2090-2094 |
NN |
denotes |
Mcm4 |
T2402 |
2094-2095 |
HYPH |
denotes |
/ |
T2403 |
2095-2096 |
CD |
denotes |
6 |
T2404 |
2096-2097 |
HYPH |
denotes |
/ |
T2405 |
2097-2098 |
CD |
denotes |
7 |
T2406 |
2107-2109 |
IN |
denotes |
at |
T2407 |
2110-2112 |
CD |
denotes |
37 |
T2408 |
2112-2114 |
NN |
denotes |
°C |
T2409 |
2115-2118 |
IN |
denotes |
for |
T2410 |
2119-2120 |
CD |
denotes |
1 |
T2411 |
2121-2122 |
NN |
denotes |
h |
T2412 |
2123-2125 |
IN |
denotes |
in |
T2413 |
2126-2134 |
NN |
denotes |
reaction |
T2414 |
2135-2142 |
NN |
denotes |
mixture |
T2415 |
2143-2145 |
IN |
denotes |
as |
T2416 |
2146-2155 |
VBN |
denotes |
described |
T2417 |
2156-2157 |
-LRB- |
denotes |
( |
T2418 |
2157-2159 |
CD |
denotes |
11 |
T2419 |
2159-2160 |
-RRB- |
denotes |
) |
T2420 |
2160-2162 |
, |
denotes |
, |
T2421 |
2162-2165 |
CC |
denotes |
and |
T2422 |
2166-2170 |
RB |
denotes |
then |
T2423 |
2171-2180 |
NNS |
denotes |
reactions |
T2424 |
2186-2193 |
VBN |
denotes |
stopped |
T2425 |
2181-2185 |
VBD |
denotes |
were |
T2426 |
2194-2196 |
IN |
denotes |
by |
T2427 |
2197-2205 |
NN |
denotes |
addition |
T2428 |
2206-2208 |
IN |
denotes |
of |
T2429 |
2209-2213 |
NN |
denotes |
EDTA |
T2430 |
2214-2215 |
-LRB- |
denotes |
( |
T2431 |
2218-2220 |
NN |
denotes |
mM |
T2432 |
2215-2217 |
CD |
denotes |
20 |
T2433 |
2220-2221 |
-RRB- |
denotes |
) |
T2434 |
2221-2223 |
, |
denotes |
, |
T2435 |
2223-2226 |
NN |
denotes |
SDS |
T2436 |
2227-2228 |
-LRB- |
denotes |
( |
T2437 |
2231-2232 |
NN |
denotes |
% |
T2438 |
2228-2231 |
CD |
denotes |
0.1 |
T2439 |
2232-2233 |
-RRB- |
denotes |
) |
T2440 |
2234-2237 |
CC |
denotes |
and |
T2441 |
2238-2239 |
CD |
denotes |
2 |
T2442 |
2240-2242 |
NN |
denotes |
μg |
T2443 |
2254-2255 |
NN |
denotes |
K |
T2444 |
2243-2253 |
NN |
denotes |
proteinase |
T2445 |
2255-2257 |
, |
denotes |
, |
T2446 |
2257-2265 |
VBN |
denotes |
followed |
T2447 |
2266-2268 |
IN |
denotes |
by |
T2448 |
2269-2279 |
NN |
denotes |
incubation |
T2449 |
2280-2283 |
IN |
denotes |
for |
T2450 |
2284-2286 |
CD |
denotes |
20 |
T2451 |
2287-2290 |
NN |
denotes |
min |
T2452 |
2290-2291 |
. |
denotes |
. |
T2453 |
2291-2380 |
sentence |
denotes |
Aliquots were electrophoresed on a 6.5% polyacrylamide gel in 1× TBE at 300 V for 2.5 h. |
T2454 |
2292-2300 |
NNS |
denotes |
Aliquots |
T2455 |
2306-2321 |
VBN |
denotes |
electrophoresed |
T2456 |
2301-2305 |
VBD |
denotes |
were |
T2457 |
2322-2324 |
IN |
denotes |
on |
T2458 |
2325-2326 |
DT |
denotes |
a |
T2459 |
2347-2350 |
NN |
denotes |
gel |
T2460 |
2327-2330 |
CD |
denotes |
6.5 |
T2461 |
2330-2331 |
NN |
denotes |
% |
T2462 |
2332-2346 |
NN |
denotes |
polyacrylamide |
T2463 |
2351-2353 |
IN |
denotes |
in |
T2464 |
2354-2355 |
CD |
denotes |
1 |
T2465 |
2357-2360 |
NN |
denotes |
TBE |
T2466 |
2355-2356 |
SYM |
denotes |
× |
T2467 |
2361-2363 |
IN |
denotes |
at |
T2468 |
2364-2367 |
CD |
denotes |
300 |
T2469 |
2368-2369 |
NN |
denotes |
V |
T2470 |
2370-2373 |
IN |
denotes |
for |
T2471 |
2374-2377 |
CD |
denotes |
2.5 |
T2472 |
2378-2379 |
NN |
denotes |
h |
T2473 |
2379-2380 |
. |
denotes |
. |
T2474 |
2380-2452 |
sentence |
denotes |
The single-stranded circular DNAs used were M13mp18 and M13mp19+G-rich. |
T2475 |
2381-2384 |
DT |
denotes |
The |
T2476 |
2410-2414 |
NNS |
denotes |
DNAs |
T2477 |
2385-2391 |
JJ |
denotes |
single |
T2478 |
2392-2400 |
VBN |
denotes |
stranded |
T2479 |
2391-2392 |
HYPH |
denotes |
- |
T2480 |
2401-2409 |
JJ |
denotes |
circular |
T2481 |
2420-2424 |
VBD |
denotes |
were |
T2482 |
2415-2419 |
VBN |
denotes |
used |
T2483 |
2425-2432 |
NN |
denotes |
M13mp18 |
T2484 |
2433-2436 |
CC |
denotes |
and |
T2485 |
2437-2444 |
NN |
denotes |
M13mp19 |
T2486 |
2444-2445 |
SYM |
denotes |
+ |
T2487 |
2445-2446 |
NN |
denotes |
G |
T2488 |
2446-2447 |
HYPH |
denotes |
- |
T2489 |
2447-2451 |
JJ |
denotes |
rich |
T2490 |
2451-2452 |
. |
denotes |
. |
T2491 |
2452-2712 |
sentence |
denotes |
The latter was constructed by inserting a G-rich repeat sequence (GGGGCGTGGGC)6, prepared by annealing of two oligonucleotides (5′-GATCC-[GGGGCGTGGGC]6-3′/5′-GATCC-[GCCCACGCCCC]6-3′) at the BamHI site of M13mp19 and the sequences of the insert were confirmed. |
T2492 |
2453-2456 |
DT |
denotes |
The |
T2493 |
2457-2463 |
JJ |
denotes |
latter |
T2494 |
2468-2479 |
VBN |
denotes |
constructed |
T2495 |
2464-2467 |
VBD |
denotes |
was |
T2496 |
2480-2482 |
IN |
denotes |
by |
T2497 |
2483-2492 |
VBG |
denotes |
inserting |
T2498 |
2493-2494 |
DT |
denotes |
a |
T2499 |
2509-2517 |
NN |
denotes |
sequence |
T2500 |
2495-2496 |
NN |
denotes |
G |
T2501 |
2496-2497 |
HYPH |
denotes |
- |
T2502 |
2497-2501 |
JJ |
denotes |
rich |
T2503 |
2502-2508 |
NN |
denotes |
repeat |
T2504 |
2518-2519 |
-LRB- |
denotes |
( |
T2505 |
2519-2530 |
NN |
denotes |
GGGGCGTGGGC |
T2506 |
2530-2531 |
-RRB- |
denotes |
) |
T2507 |
2531-2532 |
CD |
denotes |
6 |
T2508 |
2532-2534 |
, |
denotes |
, |
T2509 |
2534-2542 |
VBN |
denotes |
prepared |
T2510 |
2543-2545 |
IN |
denotes |
by |
T2511 |
2546-2555 |
VBG |
denotes |
annealing |
T2512 |
2556-2558 |
IN |
denotes |
of |
T2513 |
2559-2562 |
CD |
denotes |
two |
T2514 |
2563-2579 |
NNS |
denotes |
oligonucleotides |
T2515 |
2580-2581 |
-LRB- |
denotes |
( |
T2516 |
2581-2582 |
CD |
denotes |
5 |
T2517 |
2582-2583 |
SYM |
denotes |
′ |
T2518 |
2583-2584 |
HYPH |
denotes |
- |
T2519 |
2584-2589 |
NN |
denotes |
GATCC |
T2520 |
2591-2602 |
NN |
denotes |
GGGGCGTGGGC |
T2521 |
2589-2590 |
HYPH |
denotes |
- |
T2522 |
2590-2591 |
-LRB- |
denotes |
[ |
T2523 |
2602-2603 |
-RRB- |
denotes |
] |
T2524 |
2603-2604 |
CD |
denotes |
6 |
T2525 |
2604-2605 |
HYPH |
denotes |
- |
T2526 |
2605-2606 |
CD |
denotes |
3 |
T2527 |
2606-2607 |
SYM |
denotes |
′ |
T2528 |
2607-2608 |
HYPH |
denotes |
/ |
T2529 |
2608-2609 |
CD |
denotes |
5 |
T2530 |
2609-2610 |
SYM |
denotes |
′ |
T2531 |
2610-2611 |
HYPH |
denotes |
- |
T2532 |
2611-2616 |
NN |
denotes |
GATCC |
T2533 |
2618-2629 |
NN |
denotes |
GCCCACGCCCC |
T2534 |
2616-2617 |
HYPH |
denotes |
- |
T2535 |
2617-2618 |
-LRB- |
denotes |
[ |
T2536 |
2629-2630 |
-RRB- |
denotes |
] |
T2537 |
2630-2631 |
CD |
denotes |
6 |
T2538 |
2631-2632 |
HYPH |
denotes |
- |
T2539 |
2632-2633 |
CD |
denotes |
3 |
T2540 |
2633-2634 |
SYM |
denotes |
′ |
T2541 |
2634-2635 |
-RRB- |
denotes |
) |
T2542 |
2636-2638 |
IN |
denotes |
at |
T2543 |
2639-2642 |
DT |
denotes |
the |
T2544 |
2649-2653 |
NN |
denotes |
site |
T2545 |
2643-2648 |
NN |
denotes |
BamHI |
T2546 |
2654-2656 |
IN |
denotes |
of |
T2547 |
2657-2664 |
NN |
denotes |
M13mp19 |
T2548 |
2665-2668 |
CC |
denotes |
and |
T2549 |
2669-2672 |
DT |
denotes |
the |
T2550 |
2673-2682 |
NNS |
denotes |
sequences |
T2551 |
2702-2711 |
VBN |
denotes |
confirmed |
T2552 |
2683-2685 |
IN |
denotes |
of |
T2553 |
2686-2689 |
DT |
denotes |
the |
T2554 |
2690-2696 |
NN |
denotes |
insert |
T2555 |
2697-2701 |
VBD |
denotes |
were |
T2556 |
2711-2712 |
. |
denotes |
. |
R1124 |
T1980 |
T1981 |
compound |
DNA,substrates |
R1125 |
T1983 |
T1984 |
det |
The,sequences |
R1126 |
T1984 |
T1985 |
nsubjpass |
sequences,listed |
R1127 |
T1986 |
T1984 |
prep |
for,sequences |
R1128 |
T1987 |
T1988 |
predet |
all,oligonucleotides |
R1129 |
T1988 |
T1986 |
pobj |
oligonucleotides,for |
R1130 |
T1989 |
T1988 |
det |
the,oligonucleotides |
R1131 |
T1990 |
T1988 |
acl |
used,oligonucleotides |
R1132 |
T1991 |
T1990 |
prep |
for,used |
R1133 |
T1992 |
T1991 |
pobj |
constructions,for |
R1134 |
T1993 |
T1992 |
prep |
of,constructions |
R1135 |
T1994 |
T1995 |
compound |
DNA,substrates |
R1136 |
T1995 |
T1993 |
pobj |
substrates,of |
R1137 |
T1996 |
T1985 |
auxpass |
are,listed |
R1138 |
T1997 |
T1985 |
prep |
in,listed |
R1139 |
T1998 |
T1999 |
nmod |
Tables,1 |
R1140 |
T1999 |
T1997 |
pobj |
1,in |
R1141 |
T2000 |
T1999 |
cc |
and,1 |
R1142 |
T2001 |
T1999 |
conj |
2,1 |
R1143 |
T2002 |
T1985 |
punct |
.,listed |
R1144 |
T2004 |
T2005 |
det |
The,substrates |
R1145 |
T2005 |
T2008 |
nsubjpass |
substrates,constructed |
R1146 |
T2006 |
T2005 |
amod |
partial,substrates |
R1147 |
T2007 |
T2005 |
compound |
heteroduplex,substrates |
R1148 |
T2009 |
T2008 |
auxpass |
were,constructed |
R1149 |
T2010 |
T2008 |
prep |
by,constructed |
R1150 |
T2011 |
T2010 |
pcomp |
annealing,by |
R1151 |
T2012 |
T2013 |
amod |
labeled,oligonucleotides |
R1152 |
T2013 |
T2011 |
dobj |
oligonucleotides,annealing |
R1153 |
T2014 |
T2011 |
prep |
with,annealing |
R1154 |
T2015 |
T2016 |
nmod |
M13mp18,DNA |
R1155 |
T2016 |
T2014 |
pobj |
DNA,with |
R1156 |
T2017 |
T2018 |
amod |
single,stranded |
R1157 |
T2018 |
T2016 |
amod |
stranded,DNA |
R1158 |
T2019 |
T2018 |
punct |
-,stranded |
R1159 |
T2020 |
T2016 |
amod |
circular,DNA |
R1160 |
T2021 |
T2016 |
cc |
or,DNA |
R1161 |
T2022 |
T2023 |
poss |
its,derivative |
R1162 |
T2023 |
T2016 |
conj |
derivative,DNA |
R1163 |
T2024 |
T2008 |
punct |
.,constructed |
R1164 |
T2026 |
T2027 |
det |
The,substrates |
R1165 |
T2027 |
T2029 |
nsubjpass |
substrates,purified |
R1166 |
T2028 |
T2027 |
amod |
labeled,substrates |
R1167 |
T2030 |
T2029 |
auxpass |
were,purified |
R1168 |
T2031 |
T2029 |
agent |
by,purified |
R1169 |
T2032 |
T2033 |
compound |
Sepharose,CL4B |
R1170 |
T2033 |
T2034 |
compound |
CL4B,chromatography |
R1171 |
T2034 |
T2031 |
pobj |
chromatography,by |
R1172 |
T2035 |
T2034 |
compound |
column,chromatography |
R1173 |
T2036 |
T2037 |
punct |
(,Biotech |
R1174 |
T2037 |
T2029 |
parataxis |
Biotech,purified |
R1175 |
T2038 |
T2037 |
compound |
Amersham,Biotech |
R1176 |
T2039 |
T2037 |
compound |
Pharmacia,Biotech |
R1177 |
T2040 |
T2037 |
punct |
),Biotech |
R1178 |
T2041 |
T2029 |
punct |
.,purified |
R1179 |
T2043 |
T2044 |
det |
The,substrates |
R1180 |
T2044 |
T2047 |
nsubjpass |
substrates,assembled |
R1181 |
T2045 |
T2046 |
compound |
DNA,bubble |
R1182 |
T2046 |
T2044 |
compound |
bubble,substrates |
R1183 |
T2048 |
T2047 |
auxpass |
were,assembled |
R1184 |
T2049 |
T2047 |
prep |
from,assembled |
R1185 |
T2050 |
T2051 |
nummod |
two,oligonucleotides |
R1186 |
T2051 |
T2049 |
pobj |
oligonucleotides,from |
R1187 |
T2052 |
T2053 |
advmod |
partially,complementary |
R1188 |
T2053 |
T2051 |
amod |
complementary,oligonucleotides |
R1189 |
T2054 |
T2051 |
prep |
with,oligonucleotides |
R1190 |
T2055 |
T2056 |
amod |
top,sequences |
R1191 |
T2056 |
T2054 |
pobj |
sequences,with |
R1192 |
T2057 |
T2055 |
cc |
and,top |
R1193 |
T2058 |
T2055 |
conj |
bottom,top |
R1194 |
T2059 |
T2056 |
compound |
strand,sequences |
R1195 |
T2060 |
T2061 |
punct |
(,Table |
R1196 |
T2061 |
T2051 |
parataxis |
Table,oligonucleotides |
R1197 |
T2062 |
T2061 |
nummod |
1,Table |
R1198 |
T2063 |
T2061 |
punct |
),Table |
R1199 |
T2064 |
T2047 |
punct |
.,assembled |
R1200 |
T2066 |
T2067 |
npadvmod |
T,tailed |
R1201 |
T2067 |
T2069 |
amod |
tailed,substrates |
R1202 |
T2068 |
T2067 |
punct |
-,tailed |
R1203 |
T2069 |
T2073 |
nsubjpass |
substrates,prepared |
R1204 |
T2070 |
T2071 |
compound |
Y,fork |
R1205 |
T2071 |
T2069 |
compound |
fork,substrates |
R1206 |
T2072 |
T2071 |
punct |
-,fork |
R1207 |
T2074 |
T2073 |
auxpass |
were,prepared |
R1208 |
T2075 |
T2073 |
prep |
by,prepared |
R1209 |
T2076 |
T2075 |
pcomp |
annealing,by |
R1210 |
T2077 |
T2078 |
nummod |
two,oligonucleotides |
R1211 |
T2078 |
T2076 |
dobj |
oligonucleotides,annealing |
R1212 |
T2079 |
T2080 |
punct |
(,series |
R1213 |
T2080 |
T2078 |
parataxis |
series,oligonucleotides |
R1214 |
T2081 |
T2080 |
nmod |
dT30,series |
R1215 |
T2082 |
T2081 |
cc |
and,dT30 |
R1216 |
T2083 |
T2081 |
conj |
dT60,dT30 |
R1217 |
T2084 |
T2080 |
punct |
),series |
R1218 |
T2085 |
T2073 |
punct |
.,prepared |
R1219 |
T2087 |
T2088 |
aux |
To,construct |
R1220 |
T2088 |
T2089 |
advcl |
construct,annealed |
R1221 |
T2090 |
T2091 |
det |
the,fork |
R1222 |
T2091 |
T2088 |
dobj |
fork,construct |
R1223 |
T2092 |
T2091 |
amod |
arrested,fork |
R1224 |
T2093 |
T2091 |
cc |
and,fork |
R1225 |
T2094 |
T2095 |
nummod |
3,substrates |
R1226 |
T2095 |
T2091 |
conj |
substrates,fork |
R1227 |
T2096 |
T2094 |
punct |
′,3 |
R1228 |
T2097 |
T2094 |
punct |
-,3 |
R1229 |
T2098 |
T2094 |
cc |
or,3 |
R1230 |
T2099 |
T2094 |
conj |
5,3 |
R1231 |
T2100 |
T2099 |
punct |
′,5 |
R1232 |
T2101 |
T2095 |
punct |
-,substrates |
R1233 |
T2102 |
T2095 |
compound |
extension,substrates |
R1234 |
T2103 |
T2089 |
punct |
", ",annealed |
R1235 |
T2104 |
T2105 |
amod |
various,combinations |
R1236 |
T2105 |
T2089 |
nsubjpass |
combinations,annealed |
R1237 |
T2106 |
T2105 |
prep |
of,combinations |
R1238 |
T2107 |
T2106 |
pobj |
oligonucleotides,of |
R1239 |
T2108 |
T2107 |
acl |
shown,oligonucleotides |
R1240 |
T2109 |
T2108 |
prep |
in,shown |
R1241 |
T2110 |
T2109 |
pobj |
Table,in |
R1242 |
T2111 |
T2110 |
nummod |
1,Table |
R1243 |
T2112 |
T2089 |
auxpass |
were,annealed |
R1244 |
T2113 |
T2089 |
punct |
.,annealed |
R1245 |
T2115 |
T2116 |
nmod |
Bub,B2 |
R1246 |
T2116 |
T2121 |
nsubjpass |
B2,constructed |
R1247 |
T2117 |
T2116 |
punct |
-,B2 |
R1248 |
T2118 |
T2116 |
nummod |
82,B2 |
R1249 |
T2119 |
T2116 |
punct |
/,B2 |
R1250 |
T2120 |
T2116 |
compound |
lamin,B2 |
R1251 |
T2122 |
T2116 |
acl |
containing,B2 |
R1252 |
T2123 |
T2124 |
det |
the,strand |
R1253 |
T2124 |
T2122 |
dobj |
strand,containing |
R1254 |
T2125 |
T2126 |
npadvmod |
T,rich |
R1255 |
T2126 |
T2124 |
amod |
rich,strand |
R1256 |
T2127 |
T2126 |
punct |
-,rich |
R1257 |
T2128 |
T2124 |
prep |
of,strand |
R1258 |
T2129 |
T2130 |
det |
the,region |
R1259 |
T2130 |
T2128 |
pobj |
region,of |
R1260 |
T2131 |
T2132 |
compound |
lamin,B2 |
R1261 |
T2132 |
T2130 |
compound |
B2,region |
R1262 |
T2133 |
T2130 |
compound |
origin,region |
R1263 |
T2134 |
T2121 |
auxpass |
was,constructed |
R1264 |
T2135 |
T2136 |
mark |
as,described |
R1265 |
T2136 |
T2121 |
advcl |
described,constructed |
R1266 |
T2137 |
T2136 |
advmod |
previously,described |
R1267 |
T2138 |
T2139 |
punct |
(,13 |
R1268 |
T2139 |
T2136 |
parataxis |
13,described |
R1269 |
T2140 |
T2139 |
punct |
),13 |
R1270 |
T2141 |
T2121 |
punct |
.,constructed |
R1271 |
T2143 |
T2144 |
det |
The,substrate |
R1272 |
T2144 |
T2146 |
nsubj |
substrate,contains |
R1273 |
T2145 |
T2144 |
compound |
bubble,substrate |
R1274 |
T2147 |
T2144 |
punct |
", ",substrate |
R1275 |
T2148 |
T2149 |
compound |
c,myc |
R1276 |
T2149 |
T2151 |
compound |
myc,DUE |
R1277 |
T2150 |
T2149 |
punct |
-,myc |
R1278 |
T2151 |
T2144 |
appos |
DUE,substrate |
R1279 |
T2152 |
T2151 |
punct |
/,DUE |
R1280 |
T2153 |
T2151 |
cc |
or,DUE |
R1281 |
T2154 |
T2155 |
compound |
c,myc |
R1282 |
T2155 |
T2157 |
compound |
myc,C |
R1283 |
T2156 |
T2155 |
punct |
-,myc |
R1284 |
T2157 |
T2151 |
conj |
C,DUE |
R1285 |
T2158 |
T2157 |
punct |
/,C |
R1286 |
T2159 |
T2157 |
compound |
DUE,C |
R1287 |
T2160 |
T2157 |
punct |
-,C |
R1288 |
T2161 |
T2146 |
punct |
", ",contains |
R1289 |
T2162 |
T2146 |
dobj |
sequences,contains |
R1290 |
T2163 |
T2164 |
punct |
(,K01908 |
R1291 |
T2164 |
T2162 |
parataxis |
K01908,sequences |
R1292 |
T2165 |
T2166 |
compound |
GenBank,number |
R1293 |
T2166 |
T2164 |
compound |
number,K01908 |
R1294 |
T2167 |
T2166 |
compound |
accession,number |
R1295 |
T2168 |
T2164 |
punct |
),K01908 |
R1296 |
T2169 |
T2162 |
prep |
from,sequences |
R1297 |
T2170 |
T2171 |
nmod |
nucleotides,853 |
R1298 |
T2171 |
T2169 |
pobj |
853,from |
R1299 |
T2172 |
T2171 |
nummod |
722,853 |
R1300 |
T2173 |
T2171 |
nmod |
to,853 |
R1301 |
T2174 |
T2162 |
cc |
or,sequences |
R1302 |
T2175 |
T2162 |
conj |
those,sequences |
R1303 |
T2176 |
T2175 |
prep |
from,those |
R1304 |
T2177 |
T2176 |
pobj |
828,from |
R1305 |
T2178 |
T2176 |
prep |
to,from |
R1306 |
T2179 |
T2178 |
pobj |
747,to |
R1307 |
T2180 |
T2181 |
punct |
(,strand |
R1308 |
T2181 |
T2175 |
parataxis |
strand,those |
R1309 |
T2182 |
T2181 |
amod |
complementary,strand |
R1310 |
T2183 |
T2181 |
punct |
),strand |
R1311 |
T2184 |
T2146 |
punct |
", ",contains |
R1312 |
T2185 |
T2146 |
advmod |
respectively,contains |
R1313 |
T2186 |
T2146 |
punct |
", ",contains |
R1314 |
T2187 |
T2146 |
prep |
in,contains |
R1315 |
T2188 |
T2189 |
det |
the,region |
R1316 |
T2189 |
T2187 |
pobj |
region,in |
R1317 |
T2190 |
T2189 |
amod |
central,region |
R1318 |
T2191 |
T2189 |
amod |
melted,region |
R1319 |
T2192 |
T2146 |
punct |
.,contains |
R1320 |
T2194 |
T2195 |
prep |
For,annealed |
R1321 |
T2196 |
T2197 |
det |
each,substrate |
R1322 |
T2197 |
T2194 |
pobj |
substrate,For |
R1323 |
T2198 |
T2195 |
punct |
", ",annealed |
R1324 |
T2199 |
T2200 |
amod |
labeled,oligonucleotide |
R1325 |
T2200 |
T2195 |
nsubjpass |
oligonucleotide,annealed |
R1326 |
T2201 |
T2202 |
punct |
(,pmol |
R1327 |
T2202 |
T2200 |
parataxis |
pmol,oligonucleotide |
R1328 |
T2203 |
T2202 |
nummod |
3,pmol |
R1329 |
T2204 |
T2202 |
punct |
),pmol |
R1330 |
T2205 |
T2195 |
auxpass |
was,annealed |
R1331 |
T2206 |
T2195 |
prep |
with,annealed |
R1332 |
T2207 |
T2208 |
det |
the,oligonucleotide |
R1333 |
T2208 |
T2206 |
pobj |
oligonucleotide,with |
R1334 |
T2209 |
T2208 |
amod |
unlabeled,oligonucleotide |
R1335 |
T2210 |
T2211 |
punct |
(,pmol |
R1336 |
T2211 |
T2208 |
parataxis |
pmol,oligonucleotide |
R1337 |
T2212 |
T2211 |
nummod |
6,pmol |
R1338 |
T2213 |
T2211 |
punct |
),pmol |
R1339 |
T2214 |
T2195 |
prep |
in,annealed |
R1340 |
T2215 |
T2216 |
det |
a,mixture |
R1341 |
T2216 |
T2214 |
pobj |
mixture,in |
R1342 |
T2217 |
T2216 |
compound |
reaction,mixture |
R1343 |
T2218 |
T2219 |
punct |
(,μl |
R1344 |
T2219 |
T2216 |
parataxis |
μl,mixture |
R1345 |
T2220 |
T2219 |
nummod |
50,μl |
R1346 |
T2221 |
T2219 |
punct |
),μl |
R1347 |
T2222 |
T2216 |
acl |
containing,mixture |
R1348 |
T2223 |
T2224 |
nummod |
20,mM |
R1349 |
T2224 |
T2225 |
compound |
mM,HCl |
R1350 |
T2225 |
T2222 |
dobj |
HCl,containing |
R1351 |
T2226 |
T2225 |
compound |
Tris,HCl |
R1352 |
T2227 |
T2225 |
punct |
–,HCl |
R1353 |
T2228 |
T2229 |
punct |
(,pH |
R1354 |
T2229 |
T2225 |
parataxis |
pH,HCl |
R1355 |
T2230 |
T2229 |
nummod |
7.5,pH |
R1356 |
T2231 |
T2229 |
punct |
),pH |
R1357 |
T2232 |
T2225 |
punct |
", ",HCl |
R1358 |
T2233 |
T2234 |
nummod |
10,mM |
R1359 |
T2234 |
T2235 |
compound |
mM,MgCl2 |
R1360 |
T2235 |
T2225 |
conj |
MgCl2,HCl |
R1361 |
T2236 |
T2235 |
cc |
and,MgCl2 |
R1362 |
T2237 |
T2238 |
nummod |
25,mM |
R1363 |
T2238 |
T2239 |
compound |
mM,NaCl |
R1364 |
T2239 |
T2235 |
conj |
NaCl,MgCl2 |
R1365 |
T2240 |
T2216 |
punct |
", ",mixture |
R1366 |
T2241 |
T2242 |
dep |
which,heated |
R1367 |
T2242 |
T2216 |
relcl |
heated,mixture |
R1368 |
T2243 |
T2242 |
auxpass |
were,heated |
R1369 |
T2244 |
T2242 |
prep |
to,heated |
R1370 |
T2245 |
T2246 |
nummod |
95,°C |
R1371 |
T2246 |
T2244 |
pobj |
°C,to |
R1372 |
T2247 |
T2242 |
punct |
", ",heated |
R1373 |
T2248 |
T2242 |
conj |
kept,heated |
R1374 |
T2249 |
T2248 |
prep |
at,kept |
R1375 |
T2250 |
T2251 |
nummod |
67,°C |
R1376 |
T2251 |
T2249 |
pobj |
°C,at |
R1377 |
T2252 |
T2248 |
prep |
for,kept |
R1378 |
T2253 |
T2254 |
nummod |
1,h |
R1379 |
T2254 |
T2252 |
pobj |
h,for |
R1380 |
T2255 |
T2248 |
punct |
", ",kept |
R1381 |
T2256 |
T2248 |
cc |
and,kept |
R1382 |
T2257 |
T2258 |
advmod |
then,allowed |
R1383 |
T2258 |
T2248 |
conj |
allowed,kept |
R1384 |
T2259 |
T2260 |
aux |
to,cool |
R1385 |
T2260 |
T2258 |
xcomp |
cool,allowed |
R1386 |
T2261 |
T2260 |
advmod |
slowly,cool |
R1387 |
T2262 |
T2260 |
prt |
down,cool |
R1388 |
T2263 |
T2260 |
prep |
to,cool |
R1389 |
T2264 |
T2265 |
nummod |
37,°C |
R1390 |
T2265 |
T2263 |
pobj |
°C,to |
R1391 |
T2266 |
T2195 |
punct |
.,annealed |
R1392 |
T2268 |
T2269 |
det |
The,substrates |
R1393 |
T2269 |
T2271 |
nsubjpass |
substrates,purified |
R1394 |
T2270 |
T2269 |
amod |
assembled,substrates |
R1395 |
T2272 |
T2271 |
auxpass |
were,purified |
R1396 |
T2273 |
T2271 |
prep |
from,purified |
R1397 |
T2274 |
T2275 |
compound |
polyacrylamide,gel |
R1398 |
T2275 |
T2273 |
pobj |
gel,from |
R1399 |
T2276 |
T2271 |
prep |
by,purified |
R1400 |
T2277 |
T2276 |
pobj |
elution,by |
R1401 |
T2278 |
T2277 |
prep |
into,elution |
R1402 |
T2279 |
T2280 |
compound |
TE,buffer |
R1403 |
T2280 |
T2278 |
pobj |
buffer,into |
R1404 |
T2281 |
T2282 |
punct |
(,24 |
R1405 |
T2282 |
T2271 |
parataxis |
24,purified |
R1406 |
T2283 |
T2282 |
punct |
),24 |
R1407 |
T2284 |
T2271 |
punct |
.,purified |
R1408 |
T2286 |
T2287 |
det |
The,substrates |
R1409 |
T2287 |
T2290 |
nsubjpass |
substrates,prepared |
R1410 |
T2288 |
T2287 |
amod |
partial,substrates |
R1411 |
T2289 |
T2287 |
compound |
heteroduplex,substrates |
R1412 |
T2291 |
T2287 |
prep |
on,substrates |
R1413 |
T2292 |
T2293 |
det |
a,DNA |
R1414 |
T2293 |
T2291 |
pobj |
DNA,on |
R1415 |
T2294 |
T2295 |
amod |
single,stranded |
R1416 |
T2295 |
T2293 |
amod |
stranded,DNA |
R1417 |
T2296 |
T2295 |
punct |
-,stranded |
R1418 |
T2297 |
T2293 |
amod |
circular,DNA |
R1419 |
T2298 |
T2293 |
prep |
with,DNA |
R1420 |
T2299 |
T2300 |
amod |
varied,lengths |
R1421 |
T2300 |
T2298 |
pobj |
lengths,with |
R1422 |
T2301 |
T2300 |
prep |
of,lengths |
R1423 |
T2302 |
T2303 |
compound |
duplex,regions |
R1424 |
T2303 |
T2301 |
pobj |
regions,of |
R1425 |
T2304 |
T2290 |
auxpass |
were,prepared |
R1426 |
T2305 |
T2290 |
prep |
by,prepared |
R1427 |
T2306 |
T2305 |
pcomp |
extending,by |
R1428 |
T2307 |
T2308 |
compound |
DNA,chains |
R1429 |
T2308 |
T2306 |
dobj |
chains,extending |
R1430 |
T2309 |
T2306 |
prep |
from,extending |
R1431 |
T2310 |
T2311 |
det |
the,end |
R1432 |
T2311 |
T2309 |
pobj |
end,from |
R1433 |
T2312 |
T2311 |
nummod |
3,end |
R1434 |
T2313 |
T2312 |
punct |
′,3 |
R1435 |
T2314 |
T2311 |
prep |
of,end |
R1436 |
T2315 |
T2316 |
det |
the,37mer |
R1437 |
T2316 |
T2314 |
pobj |
37mer,of |
R1438 |
T2317 |
T2316 |
compound |
dT40,37mer |
R1439 |
T2318 |
T2316 |
punct |
-,37mer |
R1440 |
T2319 |
T2316 |
punct |
(,37mer |
R1441 |
T2320 |
T2321 |
compound |
37mer,region |
R1442 |
T2321 |
T2316 |
appos |
region,37mer |
R1443 |
T2322 |
T2321 |
acl |
hybridizing,region |
R1444 |
T2323 |
T2322 |
prep |
at,hybridizing |
R1445 |
T2324 |
T2325 |
nmod |
positions,6289 |
R1446 |
T2325 |
T2323 |
pobj |
6289,at |
R1447 |
T2326 |
T2327 |
punct |
–,6326 |
R1448 |
T2327 |
T2325 |
prep |
6326,6289 |
R1449 |
T2328 |
T2325 |
prep |
of,6289 |
R1450 |
T2329 |
T2330 |
compound |
M13mp18,vector |
R1451 |
T2330 |
T2328 |
pobj |
vector,of |
R1452 |
T2331 |
T2316 |
punct |
),37mer |
R1453 |
T2332 |
T2316 |
acl |
annealed,37mer |
R1454 |
T2333 |
T2332 |
prep |
to,annealed |
R1455 |
T2334 |
T2335 |
det |
the,DNA |
R1456 |
T2335 |
T2333 |
pobj |
DNA,to |
R1457 |
T2336 |
T2337 |
amod |
single,stranded |
R1458 |
T2337 |
T2335 |
amod |
stranded,DNA |
R1459 |
T2338 |
T2337 |
punct |
-,stranded |
R1460 |
T2339 |
T2335 |
amod |
circular,DNA |
R1461 |
T2340 |
T2290 |
punct |
.,prepared |
R1462 |
T2342 |
T2343 |
compound |
DNA,chains |
R1463 |
T2343 |
T2344 |
nsubjpass |
chains,elongated |
R1464 |
T2345 |
T2344 |
auxpass |
were,elongated |
R1465 |
T2346 |
T2344 |
prep |
with,elongated |
R1466 |
T2347 |
T2346 |
pobj |
Sequenase,with |
R1467 |
T2348 |
T2344 |
prep |
in,elongated |
R1468 |
T2349 |
T2350 |
det |
the,presence |
R1469 |
T2350 |
T2348 |
pobj |
presence,in |
R1470 |
T2351 |
T2350 |
prep |
of,presence |
R1471 |
T2352 |
T2353 |
punct |
[,dGTP |
R1472 |
T2353 |
T2351 |
pobj |
dGTP,of |
R1473 |
T2354 |
T2355 |
nmod |
α,32P |
R1474 |
T2355 |
T2353 |
nmod |
32P,dGTP |
R1475 |
T2356 |
T2355 |
punct |
-,32P |
R1476 |
T2357 |
T2353 |
punct |
],dGTP |
R1477 |
T2358 |
T2353 |
punct |
", ",dGTP |
R1478 |
T2359 |
T2353 |
acl |
followed,dGTP |
R1479 |
T2360 |
T2359 |
agent |
by,followed |
R1480 |
T2361 |
T2360 |
pobj |
extension,by |
R1481 |
T2362 |
T2359 |
prep |
after,followed |
R1482 |
T2363 |
T2362 |
pobj |
addition,after |
R1483 |
T2364 |
T2363 |
prep |
of,addition |
R1484 |
T2365 |
T2364 |
pobj |
ddGTP,of |
R1485 |
T2366 |
T2365 |
cc |
and,ddGTP |
R1486 |
T2367 |
T2368 |
det |
all,dNTPs |
R1487 |
T2368 |
T2365 |
conj |
dNTPs,ddGTP |
R1488 |
T2369 |
T2368 |
nummod |
four,dNTPs |
R1489 |
T2370 |
T2344 |
punct |
", ",elongated |
R1490 |
T2371 |
T2344 |
advcl |
resulting,elongated |
R1491 |
T2372 |
T2371 |
prep |
in,resulting |
R1492 |
T2373 |
T2372 |
pobj |
substrates,in |
R1493 |
T2374 |
T2373 |
acl |
containing,substrates |
R1494 |
T2375 |
T2376 |
amod |
labeled,regions |
R1495 |
T2376 |
T2374 |
dobj |
regions,containing |
R1496 |
T2377 |
T2376 |
compound |
duplex,regions |
R1497 |
T2378 |
T2376 |
prep |
of,regions |
R1498 |
T2379 |
T2380 |
amod |
varied,lengths |
R1499 |
T2380 |
T2378 |
pobj |
lengths,of |
R1500 |
T2381 |
T2382 |
punct |
(,13 |
R1501 |
T2382 |
T2344 |
parataxis |
13,elongated |
R1502 |
T2383 |
T2382 |
punct |
),13 |
R1503 |
T2384 |
T2344 |
punct |
.,elongated |
R1504 |
T2386 |
T2387 |
det |
The,substrate |
R1505 |
T2387 |
T2388 |
nsubjpass |
substrate,incubated |
R1506 |
T2389 |
T2390 |
punct |
(,fmol |
R1507 |
T2390 |
T2387 |
parataxis |
fmol,substrate |
R1508 |
T2391 |
T2390 |
nummod |
5,fmol |
R1509 |
T2392 |
T2390 |
punct |
),fmol |
R1510 |
T2393 |
T2388 |
auxpass |
was,incubated |
R1511 |
T2394 |
T2388 |
advmod |
first,incubated |
R1512 |
T2395 |
T2388 |
prep |
with,incubated |
R1513 |
T2396 |
T2397 |
amod |
indicated,amount |
R1514 |
T2397 |
T2395 |
pobj |
amount,with |
R1515 |
T2398 |
T2397 |
prep |
of,amount |
R1516 |
T2399 |
T2400 |
det |
the,complex |
R1517 |
T2400 |
T2398 |
pobj |
complex,of |
R1518 |
T2401 |
T2400 |
nmod |
Mcm4,complex |
R1519 |
T2402 |
T2401 |
punct |
/,Mcm4 |
R1520 |
T2403 |
T2401 |
nummod |
6,Mcm4 |
R1521 |
T2404 |
T2401 |
punct |
/,Mcm4 |
R1522 |
T2405 |
T2401 |
nummod |
7,Mcm4 |
R1523 |
T2406 |
T2388 |
prep |
at,incubated |
R1524 |
T2407 |
T2408 |
nummod |
37,°C |
R1525 |
T2408 |
T2406 |
pobj |
°C,at |
R1526 |
T2409 |
T2388 |
prep |
for,incubated |
R1527 |
T2410 |
T2411 |
nummod |
1,h |
R1528 |
T2411 |
T2409 |
pobj |
h,for |
R1529 |
T2412 |
T2388 |
prep |
in,incubated |
R1530 |
T2413 |
T2414 |
compound |
reaction,mixture |
R1531 |
T2414 |
T2412 |
pobj |
mixture,in |
R1532 |
T2415 |
T2416 |
mark |
as,described |
R1533 |
T2416 |
T2388 |
advcl |
described,incubated |
R1534 |
T2417 |
T2418 |
punct |
(,11 |
R1535 |
T2418 |
T2416 |
parataxis |
11,described |
R1536 |
T2419 |
T2418 |
punct |
),11 |
R1537 |
T2420 |
T2388 |
punct |
", ",incubated |
R1538 |
T2421 |
T2388 |
cc |
and,incubated |
R1539 |
T2422 |
T2421 |
advmod |
then,and |
R1540 |
T2423 |
T2424 |
nsubjpass |
reactions,stopped |
R1541 |
T2424 |
T2388 |
conj |
stopped,incubated |
R1542 |
T2425 |
T2424 |
auxpass |
were,stopped |
R1543 |
T2426 |
T2424 |
agent |
by,stopped |
R1544 |
T2427 |
T2426 |
pobj |
addition,by |
R1545 |
T2428 |
T2427 |
prep |
of,addition |
R1546 |
T2429 |
T2428 |
pobj |
EDTA,of |
R1547 |
T2430 |
T2431 |
punct |
(,mM |
R1548 |
T2431 |
T2429 |
parataxis |
mM,EDTA |
R1549 |
T2432 |
T2431 |
nummod |
20,mM |
R1550 |
T2433 |
T2431 |
punct |
),mM |
R1551 |
T2434 |
T2429 |
punct |
", ",EDTA |
R1552 |
T2435 |
T2429 |
conj |
SDS,EDTA |
R1553 |
T2436 |
T2437 |
punct |
(,% |
R1554 |
T2437 |
T2435 |
parataxis |
%,SDS |
R1555 |
T2438 |
T2437 |
nummod |
0.1,% |
R1556 |
T2439 |
T2437 |
punct |
),% |
R1557 |
T2440 |
T2435 |
cc |
and,SDS |
R1558 |
T2441 |
T2442 |
nummod |
2,μg |
R1559 |
T2442 |
T2443 |
compound |
μg,K |
R1560 |
T2443 |
T2435 |
conj |
K,SDS |
R1561 |
T2444 |
T2443 |
compound |
proteinase,K |
R1562 |
T2445 |
T2427 |
punct |
", ",addition |
R1563 |
T2446 |
T2427 |
acl |
followed,addition |
R1564 |
T2447 |
T2446 |
agent |
by,followed |
R1565 |
T2448 |
T2447 |
pobj |
incubation,by |
R1566 |
T2449 |
T2448 |
prep |
for,incubation |
R1567 |
T2450 |
T2451 |
nummod |
20,min |
R1568 |
T2451 |
T2449 |
pobj |
min,for |
R1569 |
T2452 |
T2424 |
punct |
.,stopped |
R1570 |
T2454 |
T2455 |
nsubjpass |
Aliquots,electrophoresed |
R1571 |
T2456 |
T2455 |
auxpass |
were,electrophoresed |
R1572 |
T2457 |
T2455 |
prep |
on,electrophoresed |
R1573 |
T2458 |
T2459 |
det |
a,gel |
R1574 |
T2459 |
T2457 |
pobj |
gel,on |
R1575 |
T2460 |
T2461 |
nummod |
6.5,% |
R1576 |
T2461 |
T2459 |
compound |
%,gel |
R1577 |
T2462 |
T2459 |
compound |
polyacrylamide,gel |
R1578 |
T2463 |
T2455 |
prep |
in,electrophoresed |
R1579 |
T2464 |
T2465 |
nummod |
1,TBE |
R1580 |
T2465 |
T2463 |
pobj |
TBE,in |
R1581 |
T2466 |
T2464 |
punct |
×,1 |
R1582 |
T2467 |
T2455 |
prep |
at,electrophoresed |
R1583 |
T2468 |
T2469 |
nummod |
300,V |
R1584 |
T2469 |
T2467 |
pobj |
V,at |
R1585 |
T2470 |
T2455 |
prep |
for,electrophoresed |
R1586 |
T2471 |
T2472 |
nummod |
2.5,h |
R1587 |
T2472 |
T2470 |
pobj |
h,for |
R1588 |
T2473 |
T2455 |
punct |
.,electrophoresed |
R1589 |
T2475 |
T2476 |
det |
The,DNAs |
R1590 |
T2476 |
T2481 |
nsubj |
DNAs,were |
R1591 |
T2477 |
T2478 |
amod |
single,stranded |
R1592 |
T2478 |
T2476 |
amod |
stranded,DNAs |
R1593 |
T2479 |
T2478 |
punct |
-,stranded |
R1594 |
T2480 |
T2476 |
amod |
circular,DNAs |
R1595 |
T2482 |
T2476 |
acl |
used,DNAs |
R1596 |
T2483 |
T2481 |
attr |
M13mp18,were |
R1597 |
T2484 |
T2483 |
cc |
and,M13mp18 |
R1598 |
T2485 |
T2483 |
conj |
M13mp19,M13mp18 |
R1599 |
T2486 |
T2483 |
punct |
+,M13mp18 |
R1600 |
T2487 |
T2483 |
appos |
G,M13mp18 |
R1601 |
T2488 |
T2487 |
punct |
-,G |
R1602 |
T2489 |
T2487 |
amod |
rich,G |
R1603 |
T2490 |
T2481 |
punct |
.,were |
R1604 |
T2492 |
T2493 |
det |
The,latter |
R1605 |
T2493 |
T2494 |
nsubjpass |
latter,constructed |
R1606 |
T2495 |
T2494 |
auxpass |
was,constructed |
R1607 |
T2496 |
T2494 |
prep |
by,constructed |
R1608 |
T2497 |
T2496 |
pcomp |
inserting,by |
R1609 |
T2498 |
T2499 |
det |
a,sequence |
R1610 |
T2499 |
T2497 |
dobj |
sequence,inserting |
R1611 |
T2500 |
T2499 |
nmod |
G,sequence |
R1612 |
T2501 |
T2500 |
punct |
-,G |
R1613 |
T2502 |
T2500 |
amod |
rich,G |
R1614 |
T2503 |
T2499 |
compound |
repeat,sequence |
R1615 |
T2504 |
T2499 |
punct |
(,sequence |
R1616 |
T2505 |
T2499 |
appos |
GGGGCGTGGGC,sequence |
R1617 |
T2506 |
T2505 |
punct |
),GGGGCGTGGGC |
R1618 |
T2507 |
T2505 |
nummod |
6,GGGGCGTGGGC |
R1619 |
T2508 |
T2499 |
punct |
", ",sequence |
R1620 |
T2509 |
T2499 |
acl |
prepared,sequence |
R1621 |
T2510 |
T2509 |
prep |
by,prepared |
R1622 |
T2511 |
T2510 |
pcomp |
annealing,by |
R1623 |
T2512 |
T2511 |
prep |
of,annealing |
R1624 |
T2513 |
T2514 |
nummod |
two,oligonucleotides |
R1625 |
T2514 |
T2512 |
pobj |
oligonucleotides,of |
R1626 |
T2515 |
T2514 |
punct |
(,oligonucleotides |
R1627 |
T2516 |
T2514 |
appos |
5,oligonucleotides |
R1628 |
T2517 |
T2516 |
punct |
′,5 |
R1629 |
T2518 |
T2516 |
punct |
-,5 |
R1630 |
T2519 |
T2520 |
nmod |
GATCC,GGGGCGTGGGC |
R1631 |
T2520 |
T2516 |
appos |
GGGGCGTGGGC,5 |
R1632 |
T2521 |
T2520 |
punct |
-,GGGGCGTGGGC |
R1633 |
T2522 |
T2520 |
punct |
[,GGGGCGTGGGC |
R1634 |
T2523 |
T2520 |
punct |
],GGGGCGTGGGC |
R1635 |
T2524 |
T2520 |
nummod |
6,GGGGCGTGGGC |
R1636 |
T2525 |
T2516 |
punct |
-,5 |
R1637 |
T2526 |
T2516 |
nummod |
3,5 |
R1638 |
T2527 |
T2516 |
punct |
′,5 |
R1639 |
T2528 |
T2516 |
punct |
/,5 |
R1640 |
T2529 |
T2516 |
appos |
5,5 |
R1641 |
T2530 |
T2529 |
punct |
′,5 |
R1642 |
T2531 |
T2529 |
punct |
-,5 |
R1643 |
T2532 |
T2533 |
nmod |
GATCC,GCCCACGCCCC |
R1644 |
T2533 |
T2529 |
appos |
GCCCACGCCCC,5 |
R1645 |
T2534 |
T2533 |
punct |
-,GCCCACGCCCC |
R1646 |
T2535 |
T2533 |
punct |
[,GCCCACGCCCC |
R1647 |
T2536 |
T2533 |
punct |
],GCCCACGCCCC |
R1648 |
T2537 |
T2533 |
nummod |
6,GCCCACGCCCC |
R1649 |
T2538 |
T2529 |
punct |
-,5 |
R1650 |
T2539 |
T2529 |
nummod |
3,5 |
R1651 |
T2540 |
T2529 |
punct |
′,5 |
R1652 |
T2541 |
T2514 |
punct |
),oligonucleotides |
R1653 |
T2542 |
T2497 |
prep |
at,inserting |
R1654 |
T2543 |
T2544 |
det |
the,site |
R1655 |
T2544 |
T2542 |
pobj |
site,at |
R1656 |
T2545 |
T2544 |
compound |
BamHI,site |
R1657 |
T2546 |
T2544 |
prep |
of,site |
R1658 |
T2547 |
T2546 |
pobj |
M13mp19,of |
R1659 |
T2548 |
T2494 |
cc |
and,constructed |
R1660 |
T2549 |
T2550 |
det |
the,sequences |
R1661 |
T2550 |
T2551 |
nsubjpass |
sequences,confirmed |
R1662 |
T2551 |
T2494 |
conj |
confirmed,constructed |
R1663 |
T2552 |
T2550 |
prep |
of,sequences |
R1664 |
T2553 |
T2554 |
det |
the,insert |
R1665 |
T2554 |
T2552 |
pobj |
insert,of |
R1666 |
T2555 |
T2551 |
auxpass |
were,confirmed |
R1667 |
T2556 |
T2551 |
punct |
.,confirmed |