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PMC:1140370 / 5921-8633 JSONTXT

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Id Subject Object Predicate Lexical cue
T1980 0-3 NN denotes DNA
T1981 4-14 NNS denotes substrates
T1982 14-128 sentence denotes The sequences for all the oligonucleotides used for constructions of DNA substrates are listed in Tables 1 and 2.
T1983 15-18 DT denotes The
T1984 19-28 NNS denotes sequences
T1985 103-109 VBN denotes listed
T1986 29-32 IN denotes for
T1987 33-36 PDT denotes all
T1988 41-57 NNS denotes oligonucleotides
T1989 37-40 DT denotes the
T1990 58-62 VBN denotes used
T1991 63-66 IN denotes for
T1992 67-80 NNS denotes constructions
T1993 81-83 IN denotes of
T1994 84-87 NN denotes DNA
T1995 88-98 NNS denotes substrates
T1996 99-102 VBP denotes are
T1997 110-112 IN denotes in
T1998 113-119 NNS denotes Tables
T1999 120-121 CD denotes 1
T2000 122-125 CC denotes and
T2001 126-127 CD denotes 2
T2002 127-128 . denotes .
T2003 128-280 sentence denotes The partial heteroduplex substrates were constructed by annealing labeled oligonucleotides with M13mp18 single-stranded circular DNA or its derivative.
T2004 129-132 DT denotes The
T2005 154-164 NNS denotes substrates
T2006 133-140 JJ denotes partial
T2007 141-153 NN denotes heteroduplex
T2008 170-181 VBN denotes constructed
T2009 165-169 VBD denotes were
T2010 182-184 IN denotes by
T2011 185-194 VBG denotes annealing
T2012 195-202 VBN denotes labeled
T2013 203-219 NNS denotes oligonucleotides
T2014 220-224 IN denotes with
T2015 225-232 NN denotes M13mp18
T2016 258-261 NN denotes DNA
T2017 233-239 JJ denotes single
T2018 240-248 VBN denotes stranded
T2019 239-240 HYPH denotes -
T2020 249-257 JJ denotes circular
T2021 262-264 CC denotes or
T2022 265-268 PRP$ denotes its
T2023 269-279 NN denotes derivative
T2024 279-280 . denotes .
T2025 280-387 sentence denotes The labeled substrates were purified by Sepharose CL4B column chromatography (Amersham Pharmacia Biotech).
T2026 281-284 DT denotes The
T2027 293-303 NNS denotes substrates
T2028 285-292 VBN denotes labeled
T2029 309-317 VBN denotes purified
T2030 304-308 VBD denotes were
T2031 318-320 IN denotes by
T2032 321-330 NN denotes Sepharose
T2033 331-335 NN denotes CL4B
T2034 343-357 NN denotes chromatography
T2035 336-342 NN denotes column
T2036 358-359 -LRB- denotes (
T2037 378-385 NNP denotes Biotech
T2038 359-367 NNP denotes Amersham
T2039 368-377 NNP denotes Pharmacia
T2040 385-386 -RRB- denotes )
T2041 386-387 . denotes .
T2042 387-526 sentence denotes The DNA bubble substrates were assembled from two partially complementary oligonucleotides with top and bottom strand sequences (Table 1).
T2043 388-391 DT denotes The
T2044 403-413 NNS denotes substrates
T2045 392-395 NN denotes DNA
T2046 396-402 NN denotes bubble
T2047 419-428 VBN denotes assembled
T2048 414-418 VBD denotes were
T2049 429-433 IN denotes from
T2050 434-437 CD denotes two
T2051 462-478 NNS denotes oligonucleotides
T2052 438-447 RB denotes partially
T2053 448-461 JJ denotes complementary
T2054 479-483 IN denotes with
T2055 484-487 JJ denotes top
T2056 506-515 NNS denotes sequences
T2057 488-491 CC denotes and
T2058 492-498 JJ denotes bottom
T2059 499-505 NN denotes strand
T2060 516-517 -LRB- denotes (
T2061 517-522 NN denotes Table
T2062 523-524 CD denotes 1
T2063 524-525 -RRB- denotes )
T2064 525-526 . denotes .
T2065 526-625 sentence denotes T-tailed Y-fork substrates were prepared by annealing two oligonucleotides (dT30 and dT60 series).
T2066 527-528 NN denotes T
T2067 529-535 VBN denotes tailed
T2068 528-529 HYPH denotes -
T2069 543-553 NNS denotes substrates
T2070 536-537 NN denotes Y
T2071 538-542 NN denotes fork
T2072 537-538 HYPH denotes -
T2073 559-567 VBN denotes prepared
T2074 554-558 VBD denotes were
T2075 568-570 IN denotes by
T2076 571-580 VBG denotes annealing
T2077 581-584 CD denotes two
T2078 585-601 NNS denotes oligonucleotides
T2079 602-603 -LRB- denotes (
T2080 617-623 NNS denotes series
T2081 603-607 NN denotes dT30
T2082 608-611 CC denotes and
T2083 612-616 NN denotes dT60
T2084 623-624 -RRB- denotes )
T2085 624-625 . denotes .
T2086 625-765 sentence denotes To construct the arrested fork and 3′- or 5′-extension substrates, various combinations of oligonucleotides shown in Table 1 were annealed.
T2087 626-628 TO denotes To
T2088 629-638 VB denotes construct
T2089 756-764 VBN denotes annealed
T2090 639-642 DT denotes the
T2091 652-656 NN denotes fork
T2092 643-651 VBN denotes arrested
T2093 657-660 CC denotes and
T2094 661-662 CD denotes 3
T2095 681-691 NNS denotes substrates
T2096 662-663 SYM denotes
T2097 663-664 HYPH denotes -
T2098 665-667 CC denotes or
T2099 668-669 CD denotes 5
T2100 669-670 SYM denotes
T2101 670-671 HYPH denotes -
T2102 671-680 NN denotes extension
T2103 691-693 , denotes ,
T2104 693-700 JJ denotes various
T2105 701-713 NNS denotes combinations
T2106 714-716 IN denotes of
T2107 717-733 NNS denotes oligonucleotides
T2108 734-739 VBN denotes shown
T2109 740-742 IN denotes in
T2110 743-748 NN denotes Table
T2111 749-750 CD denotes 1
T2112 751-755 VBD denotes were
T2113 764-765 . denotes .
T2114 765-886 sentence denotes Bub-82/lamin B2 containing the T-rich strand of the lamin B2 origin region was constructed as previously described (13).
T2115 766-769 NN denotes Bub
T2116 779-781 NN denotes B2
T2117 769-770 HYPH denotes -
T2118 770-772 CD denotes 82
T2119 772-773 HYPH denotes /
T2120 773-778 NN denotes lamin
T2121 845-856 VBN denotes constructed
T2122 782-792 VBG denotes containing
T2123 793-796 DT denotes the
T2124 804-810 NN denotes strand
T2125 797-798 NN denotes T
T2126 799-803 JJ denotes rich
T2127 798-799 HYPH denotes -
T2128 811-813 IN denotes of
T2129 814-817 DT denotes the
T2130 834-840 NN denotes region
T2131 818-823 NN denotes lamin
T2132 824-826 NN denotes B2
T2133 827-833 NN denotes origin
T2134 841-844 VBD denotes was
T2135 857-859 IN denotes as
T2136 871-880 VBN denotes described
T2137 860-870 RB denotes previously
T2138 881-882 -LRB- denotes (
T2139 882-884 CD denotes 13
T2140 884-885 -RRB- denotes )
T2141 885-886 . denotes .
T2142 886-1108 sentence denotes The bubble substrate, c-myc/DUE or c-myc/DUE-C, contains sequences (GenBank accession number K01908) from nucleotides 722 to 853 or those from 828 to 747 (complementary strand), respectively, in the central melted region.
T2143 887-890 DT denotes The
T2144 898-907 NN denotes substrate
T2145 891-897 NN denotes bubble
T2146 935-943 VBZ denotes contains
T2147 907-909 , denotes ,
T2148 909-910 NN denotes c
T2149 911-914 NN denotes myc
T2150 910-911 HYPH denotes -
T2151 915-918 NN denotes DUE
T2152 914-915 HYPH denotes /
T2153 919-921 CC denotes or
T2154 922-923 NN denotes c
T2155 924-927 NN denotes myc
T2156 923-924 HYPH denotes -
T2157 932-933 NN denotes C
T2158 927-928 HYPH denotes /
T2159 928-931 NN denotes DUE
T2160 931-932 HYPH denotes -
T2161 933-935 , denotes ,
T2162 944-953 NNS denotes sequences
T2163 954-955 -LRB- denotes (
T2164 980-986 NN denotes K01908
T2165 955-962 NNP denotes GenBank
T2166 973-979 NN denotes number
T2167 963-972 NN denotes accession
T2168 986-987 -RRB- denotes )
T2169 988-992 IN denotes from
T2170 993-1004 NNS denotes nucleotides
T2171 1012-1015 CD denotes 853
T2172 1005-1008 CD denotes 722
T2173 1009-1011 IN denotes to
T2174 1016-1018 CC denotes or
T2175 1019-1024 DT denotes those
T2176 1025-1029 IN denotes from
T2177 1030-1033 CD denotes 828
T2178 1034-1036 IN denotes to
T2179 1037-1040 CD denotes 747
T2180 1041-1042 -LRB- denotes (
T2181 1056-1062 NN denotes strand
T2182 1042-1055 JJ denotes complementary
T2183 1062-1063 -RRB- denotes )
T2184 1063-1065 , denotes ,
T2185 1065-1077 RB denotes respectively
T2186 1077-1079 , denotes ,
T2187 1079-1081 IN denotes in
T2188 1082-1085 DT denotes the
T2189 1101-1107 NN denotes region
T2190 1086-1093 JJ denotes central
T2191 1094-1100 VBN denotes melted
T2192 1107-1108 . denotes .
T2193 1108-1407 sentence denotes For each substrate, labeled oligonucleotide (3 pmol) was annealed with the unlabeled oligonucleotide (6 pmol) in a reaction mixture (50 μl) containing 20 mM Tris–HCl (pH 7.5), 10 mM MgCl2 and 25 mM NaCl, which were heated to 95°C, kept at 67°C for 1 h, and then allowed to slowly cool down to 37°C.
T2194 1109-1112 IN denotes For
T2195 1166-1174 VBN denotes annealed
T2196 1113-1117 DT denotes each
T2197 1118-1127 NN denotes substrate
T2198 1127-1129 , denotes ,
T2199 1129-1136 VBN denotes labeled
T2200 1137-1152 NN denotes oligonucleotide
T2201 1153-1154 -LRB- denotes (
T2202 1156-1160 NN denotes pmol
T2203 1154-1155 CD denotes 3
T2204 1160-1161 -RRB- denotes )
T2205 1162-1165 VBD denotes was
T2206 1175-1179 IN denotes with
T2207 1180-1183 DT denotes the
T2208 1194-1209 NN denotes oligonucleotide
T2209 1184-1193 JJ denotes unlabeled
T2210 1210-1211 -LRB- denotes (
T2211 1213-1217 NN denotes pmol
T2212 1211-1212 CD denotes 6
T2213 1217-1218 -RRB- denotes )
T2214 1219-1221 IN denotes in
T2215 1222-1223 DT denotes a
T2216 1233-1240 NN denotes mixture
T2217 1224-1232 NN denotes reaction
T2218 1241-1242 -LRB- denotes (
T2219 1245-1247 NN denotes μl
T2220 1242-1244 CD denotes 50
T2221 1247-1248 -RRB- denotes )
T2222 1249-1259 VBG denotes containing
T2223 1260-1262 CD denotes 20
T2224 1263-1265 NN denotes mM
T2225 1271-1274 NN denotes HCl
T2226 1266-1270 NN denotes Tris
T2227 1270-1271 HYPH denotes
T2228 1275-1276 -LRB- denotes (
T2229 1276-1278 NN denotes pH
T2230 1279-1282 CD denotes 7.5
T2231 1282-1283 -RRB- denotes )
T2232 1283-1285 , denotes ,
T2233 1285-1287 CD denotes 10
T2234 1288-1290 NN denotes mM
T2235 1291-1296 NN denotes MgCl2
T2236 1297-1300 CC denotes and
T2237 1301-1303 CD denotes 25
T2238 1304-1306 NN denotes mM
T2239 1307-1311 NN denotes NaCl
T2240 1311-1313 , denotes ,
T2241 1313-1318 WDT denotes which
T2242 1324-1330 VBN denotes heated
T2243 1319-1323 VBD denotes were
T2244 1331-1333 IN denotes to
T2245 1334-1336 CD denotes 95
T2246 1336-1338 NN denotes °C
T2247 1338-1340 , denotes ,
T2248 1340-1344 VBD denotes kept
T2249 1345-1347 IN denotes at
T2250 1348-1350 CD denotes 67
T2251 1350-1352 NN denotes °C
T2252 1353-1356 IN denotes for
T2253 1357-1358 CD denotes 1
T2254 1359-1360 NN denotes h
T2255 1360-1362 , denotes ,
T2256 1362-1365 CC denotes and
T2257 1366-1370 RB denotes then
T2258 1371-1378 VBN denotes allowed
T2259 1379-1381 TO denotes to
T2260 1389-1393 VB denotes cool
T2261 1382-1388 RB denotes slowly
T2262 1394-1398 RP denotes down
T2263 1399-1401 IN denotes to
T2264 1402-1404 CD denotes 37
T2265 1404-1406 NN denotes °C
T2266 1406-1407 . denotes .
T2267 1407-1502 sentence denotes The assembled substrates were purified from polyacrylamide gel by elution into TE buffer (24).
T2268 1408-1411 DT denotes The
T2269 1422-1432 NNS denotes substrates
T2270 1412-1421 VBN denotes assembled
T2271 1438-1446 VBN denotes purified
T2272 1433-1437 VBD denotes were
T2273 1447-1451 IN denotes from
T2274 1452-1466 NN denotes polyacrylamide
T2275 1467-1470 NN denotes gel
T2276 1471-1473 IN denotes by
T2277 1474-1481 NN denotes elution
T2278 1482-1486 IN denotes into
T2279 1487-1489 NN denotes TE
T2280 1490-1496 NN denotes buffer
T2281 1497-1498 -LRB- denotes (
T2282 1498-1500 CD denotes 24
T2283 1500-1501 -RRB- denotes )
T2284 1501-1502 . denotes .
T2285 1502-1796 sentence denotes The partial heteroduplex substrates on a single-stranded circular DNA with varied lengths of duplex regions were prepared by extending DNA chains from the 3′ end of the dT40-37mer (37mer region hybridizing at positions 6289–6326 of M13mp18 vector) annealed to the single-stranded circular DNA.
T2286 1503-1506 DT denotes The
T2287 1528-1538 NNS denotes substrates
T2288 1507-1514 JJ denotes partial
T2289 1515-1527 NN denotes heteroduplex
T2290 1616-1624 VBN denotes prepared
T2291 1539-1541 IN denotes on
T2292 1542-1543 DT denotes a
T2293 1569-1572 NN denotes DNA
T2294 1544-1550 JJ denotes single
T2295 1551-1559 VBN denotes stranded
T2296 1550-1551 HYPH denotes -
T2297 1560-1568 JJ denotes circular
T2298 1573-1577 IN denotes with
T2299 1578-1584 VBN denotes varied
T2300 1585-1592 NNS denotes lengths
T2301 1593-1595 IN denotes of
T2302 1596-1602 NN denotes duplex
T2303 1603-1610 NNS denotes regions
T2304 1611-1615 VBD denotes were
T2305 1625-1627 IN denotes by
T2306 1628-1637 VBG denotes extending
T2307 1638-1641 NN denotes DNA
T2308 1642-1648 NNS denotes chains
T2309 1649-1653 IN denotes from
T2310 1654-1657 DT denotes the
T2311 1661-1664 NN denotes end
T2312 1658-1659 CD denotes 3
T2313 1659-1660 SYM denotes
T2314 1665-1667 IN denotes of
T2315 1668-1671 DT denotes the
T2316 1677-1682 NN denotes 37mer
T2317 1672-1676 NN denotes dT40
T2318 1676-1677 HYPH denotes -
T2319 1683-1684 -LRB- denotes (
T2320 1684-1689 NN denotes 37mer
T2321 1690-1696 NN denotes region
T2322 1697-1708 VBG denotes hybridizing
T2323 1709-1711 IN denotes at
T2324 1712-1721 NNS denotes positions
T2325 1722-1726 CD denotes 6289
T2326 1726-1727 SYM denotes
T2327 1727-1731 CD denotes 6326
T2328 1732-1734 IN denotes of
T2329 1735-1742 NN denotes M13mp18
T2330 1743-1749 NN denotes vector
T2331 1749-1750 -RRB- denotes )
T2332 1751-1759 VBN denotes annealed
T2333 1760-1762 IN denotes to
T2334 1763-1766 DT denotes the
T2335 1792-1795 NN denotes DNA
T2336 1767-1773 JJ denotes single
T2337 1774-1782 VBN denotes stranded
T2338 1773-1774 HYPH denotes -
T2339 1783-1791 JJ denotes circular
T2340 1795-1796 . denotes .
T2341 1796-2017 sentence denotes DNA chains were elongated with Sequenase in the presence of [α-32P]dGTP, followed by extension after addition of ddGTP and all four dNTPs, resulting in substrates containing labeled duplex regions of varied lengths (13).
T2342 1797-1800 NN denotes DNA
T2343 1801-1807 NNS denotes chains
T2344 1813-1822 VBN denotes elongated
T2345 1808-1812 VBD denotes were
T2346 1823-1827 IN denotes with
T2347 1828-1837 NN denotes Sequenase
T2348 1838-1840 IN denotes in
T2349 1841-1844 DT denotes the
T2350 1845-1853 NN denotes presence
T2351 1854-1856 IN denotes of
T2352 1857-1858 -LRB- denotes [
T2353 1864-1868 NN denotes dGTP
T2354 1858-1859 NN denotes α
T2355 1860-1863 NN denotes 32P
T2356 1859-1860 HYPH denotes -
T2357 1863-1864 -RRB- denotes ]
T2358 1868-1870 , denotes ,
T2359 1870-1878 VBN denotes followed
T2360 1879-1881 IN denotes by
T2361 1882-1891 NN denotes extension
T2362 1892-1897 IN denotes after
T2363 1898-1906 NN denotes addition
T2364 1907-1909 IN denotes of
T2365 1910-1915 NN denotes ddGTP
T2366 1916-1919 CC denotes and
T2367 1920-1923 DT denotes all
T2368 1929-1934 NNS denotes dNTPs
T2369 1924-1928 CD denotes four
T2370 1934-1936 , denotes ,
T2371 1936-1945 VBG denotes resulting
T2372 1946-1948 IN denotes in
T2373 1949-1959 NNS denotes substrates
T2374 1960-1970 VBG denotes containing
T2375 1971-1978 VBN denotes labeled
T2376 1986-1993 NNS denotes regions
T2377 1979-1985 NN denotes duplex
T2378 1994-1996 IN denotes of
T2379 1997-2003 VBN denotes varied
T2380 2004-2011 NNS denotes lengths
T2381 2012-2013 -LRB- denotes (
T2382 2013-2015 CD denotes 13
T2383 2015-2016 -RRB- denotes )
T2384 2016-2017 . denotes .
T2385 2017-2291 sentence denotes The substrate (5 fmol) was first incubated with indicated amount of the Mcm4/6/7 complex at 37°C for 1 h in reaction mixture as described (11), and then reactions were stopped by addition of EDTA (20 mM), SDS (0.1%) and 2 μg proteinase K, followed by incubation for 20 min.
T2386 2018-2021 DT denotes The
T2387 2022-2031 NN denotes substrate
T2388 2051-2060 VBN denotes incubated
T2389 2032-2033 -LRB- denotes (
T2390 2035-2039 NN denotes fmol
T2391 2033-2034 CD denotes 5
T2392 2039-2040 -RRB- denotes )
T2393 2041-2044 VBD denotes was
T2394 2045-2050 RB denotes first
T2395 2061-2065 IN denotes with
T2396 2066-2075 VBN denotes indicated
T2397 2076-2082 NN denotes amount
T2398 2083-2085 IN denotes of
T2399 2086-2089 DT denotes the
T2400 2099-2106 NN denotes complex
T2401 2090-2094 NN denotes Mcm4
T2402 2094-2095 HYPH denotes /
T2403 2095-2096 CD denotes 6
T2404 2096-2097 HYPH denotes /
T2405 2097-2098 CD denotes 7
T2406 2107-2109 IN denotes at
T2407 2110-2112 CD denotes 37
T2408 2112-2114 NN denotes °C
T2409 2115-2118 IN denotes for
T2410 2119-2120 CD denotes 1
T2411 2121-2122 NN denotes h
T2412 2123-2125 IN denotes in
T2413 2126-2134 NN denotes reaction
T2414 2135-2142 NN denotes mixture
T2415 2143-2145 IN denotes as
T2416 2146-2155 VBN denotes described
T2417 2156-2157 -LRB- denotes (
T2418 2157-2159 CD denotes 11
T2419 2159-2160 -RRB- denotes )
T2420 2160-2162 , denotes ,
T2421 2162-2165 CC denotes and
T2422 2166-2170 RB denotes then
T2423 2171-2180 NNS denotes reactions
T2424 2186-2193 VBN denotes stopped
T2425 2181-2185 VBD denotes were
T2426 2194-2196 IN denotes by
T2427 2197-2205 NN denotes addition
T2428 2206-2208 IN denotes of
T2429 2209-2213 NN denotes EDTA
T2430 2214-2215 -LRB- denotes (
T2431 2218-2220 NN denotes mM
T2432 2215-2217 CD denotes 20
T2433 2220-2221 -RRB- denotes )
T2434 2221-2223 , denotes ,
T2435 2223-2226 NN denotes SDS
T2436 2227-2228 -LRB- denotes (
T2437 2231-2232 NN denotes %
T2438 2228-2231 CD denotes 0.1
T2439 2232-2233 -RRB- denotes )
T2440 2234-2237 CC denotes and
T2441 2238-2239 CD denotes 2
T2442 2240-2242 NN denotes μg
T2443 2254-2255 NN denotes K
T2444 2243-2253 NN denotes proteinase
T2445 2255-2257 , denotes ,
T2446 2257-2265 VBN denotes followed
T2447 2266-2268 IN denotes by
T2448 2269-2279 NN denotes incubation
T2449 2280-2283 IN denotes for
T2450 2284-2286 CD denotes 20
T2451 2287-2290 NN denotes min
T2452 2290-2291 . denotes .
T2453 2291-2380 sentence denotes Aliquots were electrophoresed on a 6.5% polyacrylamide gel in 1× TBE at 300 V for 2.5 h.
T2454 2292-2300 NNS denotes Aliquots
T2455 2306-2321 VBN denotes electrophoresed
T2456 2301-2305 VBD denotes were
T2457 2322-2324 IN denotes on
T2458 2325-2326 DT denotes a
T2459 2347-2350 NN denotes gel
T2460 2327-2330 CD denotes 6.5
T2461 2330-2331 NN denotes %
T2462 2332-2346 NN denotes polyacrylamide
T2463 2351-2353 IN denotes in
T2464 2354-2355 CD denotes 1
T2465 2357-2360 NN denotes TBE
T2466 2355-2356 SYM denotes ×
T2467 2361-2363 IN denotes at
T2468 2364-2367 CD denotes 300
T2469 2368-2369 NN denotes V
T2470 2370-2373 IN denotes for
T2471 2374-2377 CD denotes 2.5
T2472 2378-2379 NN denotes h
T2473 2379-2380 . denotes .
T2474 2380-2452 sentence denotes The single-stranded circular DNAs used were M13mp18 and M13mp19+G-rich.
T2475 2381-2384 DT denotes The
T2476 2410-2414 NNS denotes DNAs
T2477 2385-2391 JJ denotes single
T2478 2392-2400 VBN denotes stranded
T2479 2391-2392 HYPH denotes -
T2480 2401-2409 JJ denotes circular
T2481 2420-2424 VBD denotes were
T2482 2415-2419 VBN denotes used
T2483 2425-2432 NN denotes M13mp18
T2484 2433-2436 CC denotes and
T2485 2437-2444 NN denotes M13mp19
T2486 2444-2445 SYM denotes +
T2487 2445-2446 NN denotes G
T2488 2446-2447 HYPH denotes -
T2489 2447-2451 JJ denotes rich
T2490 2451-2452 . denotes .
T2491 2452-2712 sentence denotes The latter was constructed by inserting a G-rich repeat sequence (GGGGCGTGGGC)6, prepared by annealing of two oligonucleotides (5′-GATCC-[GGGGCGTGGGC]6-3′/5′-GATCC-[GCCCACGCCCC]6-3′) at the BamHI site of M13mp19 and the sequences of the insert were confirmed.
T2492 2453-2456 DT denotes The
T2493 2457-2463 JJ denotes latter
T2494 2468-2479 VBN denotes constructed
T2495 2464-2467 VBD denotes was
T2496 2480-2482 IN denotes by
T2497 2483-2492 VBG denotes inserting
T2498 2493-2494 DT denotes a
T2499 2509-2517 NN denotes sequence
T2500 2495-2496 NN denotes G
T2501 2496-2497 HYPH denotes -
T2502 2497-2501 JJ denotes rich
T2503 2502-2508 NN denotes repeat
T2504 2518-2519 -LRB- denotes (
T2505 2519-2530 NN denotes GGGGCGTGGGC
T2506 2530-2531 -RRB- denotes )
T2507 2531-2532 CD denotes 6
T2508 2532-2534 , denotes ,
T2509 2534-2542 VBN denotes prepared
T2510 2543-2545 IN denotes by
T2511 2546-2555 VBG denotes annealing
T2512 2556-2558 IN denotes of
T2513 2559-2562 CD denotes two
T2514 2563-2579 NNS denotes oligonucleotides
T2515 2580-2581 -LRB- denotes (
T2516 2581-2582 CD denotes 5
T2517 2582-2583 SYM denotes
T2518 2583-2584 HYPH denotes -
T2519 2584-2589 NN denotes GATCC
T2520 2591-2602 NN denotes GGGGCGTGGGC
T2521 2589-2590 HYPH denotes -
T2522 2590-2591 -LRB- denotes [
T2523 2602-2603 -RRB- denotes ]
T2524 2603-2604 CD denotes 6
T2525 2604-2605 HYPH denotes -
T2526 2605-2606 CD denotes 3
T2527 2606-2607 SYM denotes
T2528 2607-2608 HYPH denotes /
T2529 2608-2609 CD denotes 5
T2530 2609-2610 SYM denotes
T2531 2610-2611 HYPH denotes -
T2532 2611-2616 NN denotes GATCC
T2533 2618-2629 NN denotes GCCCACGCCCC
T2534 2616-2617 HYPH denotes -
T2535 2617-2618 -LRB- denotes [
T2536 2629-2630 -RRB- denotes ]
T2537 2630-2631 CD denotes 6
T2538 2631-2632 HYPH denotes -
T2539 2632-2633 CD denotes 3
T2540 2633-2634 SYM denotes
T2541 2634-2635 -RRB- denotes )
T2542 2636-2638 IN denotes at
T2543 2639-2642 DT denotes the
T2544 2649-2653 NN denotes site
T2545 2643-2648 NN denotes BamHI
T2546 2654-2656 IN denotes of
T2547 2657-2664 NN denotes M13mp19
T2548 2665-2668 CC denotes and
T2549 2669-2672 DT denotes the
T2550 2673-2682 NNS denotes sequences
T2551 2702-2711 VBN denotes confirmed
T2552 2683-2685 IN denotes of
T2553 2686-2689 DT denotes the
T2554 2690-2696 NN denotes insert
T2555 2697-2701 VBD denotes were
T2556 2711-2712 . denotes .
R1124 T1980 T1981 compound DNA,substrates
R1125 T1983 T1984 det The,sequences
R1126 T1984 T1985 nsubjpass sequences,listed
R1127 T1986 T1984 prep for,sequences
R1128 T1987 T1988 predet all,oligonucleotides
R1129 T1988 T1986 pobj oligonucleotides,for
R1130 T1989 T1988 det the,oligonucleotides
R1131 T1990 T1988 acl used,oligonucleotides
R1132 T1991 T1990 prep for,used
R1133 T1992 T1991 pobj constructions,for
R1134 T1993 T1992 prep of,constructions
R1135 T1994 T1995 compound DNA,substrates
R1136 T1995 T1993 pobj substrates,of
R1137 T1996 T1985 auxpass are,listed
R1138 T1997 T1985 prep in,listed
R1139 T1998 T1999 nmod Tables,1
R1140 T1999 T1997 pobj 1,in
R1141 T2000 T1999 cc and,1
R1142 T2001 T1999 conj 2,1
R1143 T2002 T1985 punct .,listed
R1144 T2004 T2005 det The,substrates
R1145 T2005 T2008 nsubjpass substrates,constructed
R1146 T2006 T2005 amod partial,substrates
R1147 T2007 T2005 compound heteroduplex,substrates
R1148 T2009 T2008 auxpass were,constructed
R1149 T2010 T2008 prep by,constructed
R1150 T2011 T2010 pcomp annealing,by
R1151 T2012 T2013 amod labeled,oligonucleotides
R1152 T2013 T2011 dobj oligonucleotides,annealing
R1153 T2014 T2011 prep with,annealing
R1154 T2015 T2016 nmod M13mp18,DNA
R1155 T2016 T2014 pobj DNA,with
R1156 T2017 T2018 amod single,stranded
R1157 T2018 T2016 amod stranded,DNA
R1158 T2019 T2018 punct -,stranded
R1159 T2020 T2016 amod circular,DNA
R1160 T2021 T2016 cc or,DNA
R1161 T2022 T2023 poss its,derivative
R1162 T2023 T2016 conj derivative,DNA
R1163 T2024 T2008 punct .,constructed
R1164 T2026 T2027 det The,substrates
R1165 T2027 T2029 nsubjpass substrates,purified
R1166 T2028 T2027 amod labeled,substrates
R1167 T2030 T2029 auxpass were,purified
R1168 T2031 T2029 agent by,purified
R1169 T2032 T2033 compound Sepharose,CL4B
R1170 T2033 T2034 compound CL4B,chromatography
R1171 T2034 T2031 pobj chromatography,by
R1172 T2035 T2034 compound column,chromatography
R1173 T2036 T2037 punct (,Biotech
R1174 T2037 T2029 parataxis Biotech,purified
R1175 T2038 T2037 compound Amersham,Biotech
R1176 T2039 T2037 compound Pharmacia,Biotech
R1177 T2040 T2037 punct ),Biotech
R1178 T2041 T2029 punct .,purified
R1179 T2043 T2044 det The,substrates
R1180 T2044 T2047 nsubjpass substrates,assembled
R1181 T2045 T2046 compound DNA,bubble
R1182 T2046 T2044 compound bubble,substrates
R1183 T2048 T2047 auxpass were,assembled
R1184 T2049 T2047 prep from,assembled
R1185 T2050 T2051 nummod two,oligonucleotides
R1186 T2051 T2049 pobj oligonucleotides,from
R1187 T2052 T2053 advmod partially,complementary
R1188 T2053 T2051 amod complementary,oligonucleotides
R1189 T2054 T2051 prep with,oligonucleotides
R1190 T2055 T2056 amod top,sequences
R1191 T2056 T2054 pobj sequences,with
R1192 T2057 T2055 cc and,top
R1193 T2058 T2055 conj bottom,top
R1194 T2059 T2056 compound strand,sequences
R1195 T2060 T2061 punct (,Table
R1196 T2061 T2051 parataxis Table,oligonucleotides
R1197 T2062 T2061 nummod 1,Table
R1198 T2063 T2061 punct ),Table
R1199 T2064 T2047 punct .,assembled
R1200 T2066 T2067 npadvmod T,tailed
R1201 T2067 T2069 amod tailed,substrates
R1202 T2068 T2067 punct -,tailed
R1203 T2069 T2073 nsubjpass substrates,prepared
R1204 T2070 T2071 compound Y,fork
R1205 T2071 T2069 compound fork,substrates
R1206 T2072 T2071 punct -,fork
R1207 T2074 T2073 auxpass were,prepared
R1208 T2075 T2073 prep by,prepared
R1209 T2076 T2075 pcomp annealing,by
R1210 T2077 T2078 nummod two,oligonucleotides
R1211 T2078 T2076 dobj oligonucleotides,annealing
R1212 T2079 T2080 punct (,series
R1213 T2080 T2078 parataxis series,oligonucleotides
R1214 T2081 T2080 nmod dT30,series
R1215 T2082 T2081 cc and,dT30
R1216 T2083 T2081 conj dT60,dT30
R1217 T2084 T2080 punct ),series
R1218 T2085 T2073 punct .,prepared
R1219 T2087 T2088 aux To,construct
R1220 T2088 T2089 advcl construct,annealed
R1221 T2090 T2091 det the,fork
R1222 T2091 T2088 dobj fork,construct
R1223 T2092 T2091 amod arrested,fork
R1224 T2093 T2091 cc and,fork
R1225 T2094 T2095 nummod 3,substrates
R1226 T2095 T2091 conj substrates,fork
R1227 T2096 T2094 punct ′,3
R1228 T2097 T2094 punct -,3
R1229 T2098 T2094 cc or,3
R1230 T2099 T2094 conj 5,3
R1231 T2100 T2099 punct ′,5
R1232 T2101 T2095 punct -,substrates
R1233 T2102 T2095 compound extension,substrates
R1234 T2103 T2089 punct ", ",annealed
R1235 T2104 T2105 amod various,combinations
R1236 T2105 T2089 nsubjpass combinations,annealed
R1237 T2106 T2105 prep of,combinations
R1238 T2107 T2106 pobj oligonucleotides,of
R1239 T2108 T2107 acl shown,oligonucleotides
R1240 T2109 T2108 prep in,shown
R1241 T2110 T2109 pobj Table,in
R1242 T2111 T2110 nummod 1,Table
R1243 T2112 T2089 auxpass were,annealed
R1244 T2113 T2089 punct .,annealed
R1245 T2115 T2116 nmod Bub,B2
R1246 T2116 T2121 nsubjpass B2,constructed
R1247 T2117 T2116 punct -,B2
R1248 T2118 T2116 nummod 82,B2
R1249 T2119 T2116 punct /,B2
R1250 T2120 T2116 compound lamin,B2
R1251 T2122 T2116 acl containing,B2
R1252 T2123 T2124 det the,strand
R1253 T2124 T2122 dobj strand,containing
R1254 T2125 T2126 npadvmod T,rich
R1255 T2126 T2124 amod rich,strand
R1256 T2127 T2126 punct -,rich
R1257 T2128 T2124 prep of,strand
R1258 T2129 T2130 det the,region
R1259 T2130 T2128 pobj region,of
R1260 T2131 T2132 compound lamin,B2
R1261 T2132 T2130 compound B2,region
R1262 T2133 T2130 compound origin,region
R1263 T2134 T2121 auxpass was,constructed
R1264 T2135 T2136 mark as,described
R1265 T2136 T2121 advcl described,constructed
R1266 T2137 T2136 advmod previously,described
R1267 T2138 T2139 punct (,13
R1268 T2139 T2136 parataxis 13,described
R1269 T2140 T2139 punct ),13
R1270 T2141 T2121 punct .,constructed
R1271 T2143 T2144 det The,substrate
R1272 T2144 T2146 nsubj substrate,contains
R1273 T2145 T2144 compound bubble,substrate
R1274 T2147 T2144 punct ", ",substrate
R1275 T2148 T2149 compound c,myc
R1276 T2149 T2151 compound myc,DUE
R1277 T2150 T2149 punct -,myc
R1278 T2151 T2144 appos DUE,substrate
R1279 T2152 T2151 punct /,DUE
R1280 T2153 T2151 cc or,DUE
R1281 T2154 T2155 compound c,myc
R1282 T2155 T2157 compound myc,C
R1283 T2156 T2155 punct -,myc
R1284 T2157 T2151 conj C,DUE
R1285 T2158 T2157 punct /,C
R1286 T2159 T2157 compound DUE,C
R1287 T2160 T2157 punct -,C
R1288 T2161 T2146 punct ", ",contains
R1289 T2162 T2146 dobj sequences,contains
R1290 T2163 T2164 punct (,K01908
R1291 T2164 T2162 parataxis K01908,sequences
R1292 T2165 T2166 compound GenBank,number
R1293 T2166 T2164 compound number,K01908
R1294 T2167 T2166 compound accession,number
R1295 T2168 T2164 punct ),K01908
R1296 T2169 T2162 prep from,sequences
R1297 T2170 T2171 nmod nucleotides,853
R1298 T2171 T2169 pobj 853,from
R1299 T2172 T2171 nummod 722,853
R1300 T2173 T2171 nmod to,853
R1301 T2174 T2162 cc or,sequences
R1302 T2175 T2162 conj those,sequences
R1303 T2176 T2175 prep from,those
R1304 T2177 T2176 pobj 828,from
R1305 T2178 T2176 prep to,from
R1306 T2179 T2178 pobj 747,to
R1307 T2180 T2181 punct (,strand
R1308 T2181 T2175 parataxis strand,those
R1309 T2182 T2181 amod complementary,strand
R1310 T2183 T2181 punct ),strand
R1311 T2184 T2146 punct ", ",contains
R1312 T2185 T2146 advmod respectively,contains
R1313 T2186 T2146 punct ", ",contains
R1314 T2187 T2146 prep in,contains
R1315 T2188 T2189 det the,region
R1316 T2189 T2187 pobj region,in
R1317 T2190 T2189 amod central,region
R1318 T2191 T2189 amod melted,region
R1319 T2192 T2146 punct .,contains
R1320 T2194 T2195 prep For,annealed
R1321 T2196 T2197 det each,substrate
R1322 T2197 T2194 pobj substrate,For
R1323 T2198 T2195 punct ", ",annealed
R1324 T2199 T2200 amod labeled,oligonucleotide
R1325 T2200 T2195 nsubjpass oligonucleotide,annealed
R1326 T2201 T2202 punct (,pmol
R1327 T2202 T2200 parataxis pmol,oligonucleotide
R1328 T2203 T2202 nummod 3,pmol
R1329 T2204 T2202 punct ),pmol
R1330 T2205 T2195 auxpass was,annealed
R1331 T2206 T2195 prep with,annealed
R1332 T2207 T2208 det the,oligonucleotide
R1333 T2208 T2206 pobj oligonucleotide,with
R1334 T2209 T2208 amod unlabeled,oligonucleotide
R1335 T2210 T2211 punct (,pmol
R1336 T2211 T2208 parataxis pmol,oligonucleotide
R1337 T2212 T2211 nummod 6,pmol
R1338 T2213 T2211 punct ),pmol
R1339 T2214 T2195 prep in,annealed
R1340 T2215 T2216 det a,mixture
R1341 T2216 T2214 pobj mixture,in
R1342 T2217 T2216 compound reaction,mixture
R1343 T2218 T2219 punct (,μl
R1344 T2219 T2216 parataxis μl,mixture
R1345 T2220 T2219 nummod 50,μl
R1346 T2221 T2219 punct ),μl
R1347 T2222 T2216 acl containing,mixture
R1348 T2223 T2224 nummod 20,mM
R1349 T2224 T2225 compound mM,HCl
R1350 T2225 T2222 dobj HCl,containing
R1351 T2226 T2225 compound Tris,HCl
R1352 T2227 T2225 punct –,HCl
R1353 T2228 T2229 punct (,pH
R1354 T2229 T2225 parataxis pH,HCl
R1355 T2230 T2229 nummod 7.5,pH
R1356 T2231 T2229 punct ),pH
R1357 T2232 T2225 punct ", ",HCl
R1358 T2233 T2234 nummod 10,mM
R1359 T2234 T2235 compound mM,MgCl2
R1360 T2235 T2225 conj MgCl2,HCl
R1361 T2236 T2235 cc and,MgCl2
R1362 T2237 T2238 nummod 25,mM
R1363 T2238 T2239 compound mM,NaCl
R1364 T2239 T2235 conj NaCl,MgCl2
R1365 T2240 T2216 punct ", ",mixture
R1366 T2241 T2242 dep which,heated
R1367 T2242 T2216 relcl heated,mixture
R1368 T2243 T2242 auxpass were,heated
R1369 T2244 T2242 prep to,heated
R1370 T2245 T2246 nummod 95,°C
R1371 T2246 T2244 pobj °C,to
R1372 T2247 T2242 punct ", ",heated
R1373 T2248 T2242 conj kept,heated
R1374 T2249 T2248 prep at,kept
R1375 T2250 T2251 nummod 67,°C
R1376 T2251 T2249 pobj °C,at
R1377 T2252 T2248 prep for,kept
R1378 T2253 T2254 nummod 1,h
R1379 T2254 T2252 pobj h,for
R1380 T2255 T2248 punct ", ",kept
R1381 T2256 T2248 cc and,kept
R1382 T2257 T2258 advmod then,allowed
R1383 T2258 T2248 conj allowed,kept
R1384 T2259 T2260 aux to,cool
R1385 T2260 T2258 xcomp cool,allowed
R1386 T2261 T2260 advmod slowly,cool
R1387 T2262 T2260 prt down,cool
R1388 T2263 T2260 prep to,cool
R1389 T2264 T2265 nummod 37,°C
R1390 T2265 T2263 pobj °C,to
R1391 T2266 T2195 punct .,annealed
R1392 T2268 T2269 det The,substrates
R1393 T2269 T2271 nsubjpass substrates,purified
R1394 T2270 T2269 amod assembled,substrates
R1395 T2272 T2271 auxpass were,purified
R1396 T2273 T2271 prep from,purified
R1397 T2274 T2275 compound polyacrylamide,gel
R1398 T2275 T2273 pobj gel,from
R1399 T2276 T2271 prep by,purified
R1400 T2277 T2276 pobj elution,by
R1401 T2278 T2277 prep into,elution
R1402 T2279 T2280 compound TE,buffer
R1403 T2280 T2278 pobj buffer,into
R1404 T2281 T2282 punct (,24
R1405 T2282 T2271 parataxis 24,purified
R1406 T2283 T2282 punct ),24
R1407 T2284 T2271 punct .,purified
R1408 T2286 T2287 det The,substrates
R1409 T2287 T2290 nsubjpass substrates,prepared
R1410 T2288 T2287 amod partial,substrates
R1411 T2289 T2287 compound heteroduplex,substrates
R1412 T2291 T2287 prep on,substrates
R1413 T2292 T2293 det a,DNA
R1414 T2293 T2291 pobj DNA,on
R1415 T2294 T2295 amod single,stranded
R1416 T2295 T2293 amod stranded,DNA
R1417 T2296 T2295 punct -,stranded
R1418 T2297 T2293 amod circular,DNA
R1419 T2298 T2293 prep with,DNA
R1420 T2299 T2300 amod varied,lengths
R1421 T2300 T2298 pobj lengths,with
R1422 T2301 T2300 prep of,lengths
R1423 T2302 T2303 compound duplex,regions
R1424 T2303 T2301 pobj regions,of
R1425 T2304 T2290 auxpass were,prepared
R1426 T2305 T2290 prep by,prepared
R1427 T2306 T2305 pcomp extending,by
R1428 T2307 T2308 compound DNA,chains
R1429 T2308 T2306 dobj chains,extending
R1430 T2309 T2306 prep from,extending
R1431 T2310 T2311 det the,end
R1432 T2311 T2309 pobj end,from
R1433 T2312 T2311 nummod 3,end
R1434 T2313 T2312 punct ′,3
R1435 T2314 T2311 prep of,end
R1436 T2315 T2316 det the,37mer
R1437 T2316 T2314 pobj 37mer,of
R1438 T2317 T2316 compound dT40,37mer
R1439 T2318 T2316 punct -,37mer
R1440 T2319 T2316 punct (,37mer
R1441 T2320 T2321 compound 37mer,region
R1442 T2321 T2316 appos region,37mer
R1443 T2322 T2321 acl hybridizing,region
R1444 T2323 T2322 prep at,hybridizing
R1445 T2324 T2325 nmod positions,6289
R1446 T2325 T2323 pobj 6289,at
R1447 T2326 T2327 punct –,6326
R1448 T2327 T2325 prep 6326,6289
R1449 T2328 T2325 prep of,6289
R1450 T2329 T2330 compound M13mp18,vector
R1451 T2330 T2328 pobj vector,of
R1452 T2331 T2316 punct ),37mer
R1453 T2332 T2316 acl annealed,37mer
R1454 T2333 T2332 prep to,annealed
R1455 T2334 T2335 det the,DNA
R1456 T2335 T2333 pobj DNA,to
R1457 T2336 T2337 amod single,stranded
R1458 T2337 T2335 amod stranded,DNA
R1459 T2338 T2337 punct -,stranded
R1460 T2339 T2335 amod circular,DNA
R1461 T2340 T2290 punct .,prepared
R1462 T2342 T2343 compound DNA,chains
R1463 T2343 T2344 nsubjpass chains,elongated
R1464 T2345 T2344 auxpass were,elongated
R1465 T2346 T2344 prep with,elongated
R1466 T2347 T2346 pobj Sequenase,with
R1467 T2348 T2344 prep in,elongated
R1468 T2349 T2350 det the,presence
R1469 T2350 T2348 pobj presence,in
R1470 T2351 T2350 prep of,presence
R1471 T2352 T2353 punct [,dGTP
R1472 T2353 T2351 pobj dGTP,of
R1473 T2354 T2355 nmod α,32P
R1474 T2355 T2353 nmod 32P,dGTP
R1475 T2356 T2355 punct -,32P
R1476 T2357 T2353 punct ],dGTP
R1477 T2358 T2353 punct ", ",dGTP
R1478 T2359 T2353 acl followed,dGTP
R1479 T2360 T2359 agent by,followed
R1480 T2361 T2360 pobj extension,by
R1481 T2362 T2359 prep after,followed
R1482 T2363 T2362 pobj addition,after
R1483 T2364 T2363 prep of,addition
R1484 T2365 T2364 pobj ddGTP,of
R1485 T2366 T2365 cc and,ddGTP
R1486 T2367 T2368 det all,dNTPs
R1487 T2368 T2365 conj dNTPs,ddGTP
R1488 T2369 T2368 nummod four,dNTPs
R1489 T2370 T2344 punct ", ",elongated
R1490 T2371 T2344 advcl resulting,elongated
R1491 T2372 T2371 prep in,resulting
R1492 T2373 T2372 pobj substrates,in
R1493 T2374 T2373 acl containing,substrates
R1494 T2375 T2376 amod labeled,regions
R1495 T2376 T2374 dobj regions,containing
R1496 T2377 T2376 compound duplex,regions
R1497 T2378 T2376 prep of,regions
R1498 T2379 T2380 amod varied,lengths
R1499 T2380 T2378 pobj lengths,of
R1500 T2381 T2382 punct (,13
R1501 T2382 T2344 parataxis 13,elongated
R1502 T2383 T2382 punct ),13
R1503 T2384 T2344 punct .,elongated
R1504 T2386 T2387 det The,substrate
R1505 T2387 T2388 nsubjpass substrate,incubated
R1506 T2389 T2390 punct (,fmol
R1507 T2390 T2387 parataxis fmol,substrate
R1508 T2391 T2390 nummod 5,fmol
R1509 T2392 T2390 punct ),fmol
R1510 T2393 T2388 auxpass was,incubated
R1511 T2394 T2388 advmod first,incubated
R1512 T2395 T2388 prep with,incubated
R1513 T2396 T2397 amod indicated,amount
R1514 T2397 T2395 pobj amount,with
R1515 T2398 T2397 prep of,amount
R1516 T2399 T2400 det the,complex
R1517 T2400 T2398 pobj complex,of
R1518 T2401 T2400 nmod Mcm4,complex
R1519 T2402 T2401 punct /,Mcm4
R1520 T2403 T2401 nummod 6,Mcm4
R1521 T2404 T2401 punct /,Mcm4
R1522 T2405 T2401 nummod 7,Mcm4
R1523 T2406 T2388 prep at,incubated
R1524 T2407 T2408 nummod 37,°C
R1525 T2408 T2406 pobj °C,at
R1526 T2409 T2388 prep for,incubated
R1527 T2410 T2411 nummod 1,h
R1528 T2411 T2409 pobj h,for
R1529 T2412 T2388 prep in,incubated
R1530 T2413 T2414 compound reaction,mixture
R1531 T2414 T2412 pobj mixture,in
R1532 T2415 T2416 mark as,described
R1533 T2416 T2388 advcl described,incubated
R1534 T2417 T2418 punct (,11
R1535 T2418 T2416 parataxis 11,described
R1536 T2419 T2418 punct ),11
R1537 T2420 T2388 punct ", ",incubated
R1538 T2421 T2388 cc and,incubated
R1539 T2422 T2421 advmod then,and
R1540 T2423 T2424 nsubjpass reactions,stopped
R1541 T2424 T2388 conj stopped,incubated
R1542 T2425 T2424 auxpass were,stopped
R1543 T2426 T2424 agent by,stopped
R1544 T2427 T2426 pobj addition,by
R1545 T2428 T2427 prep of,addition
R1546 T2429 T2428 pobj EDTA,of
R1547 T2430 T2431 punct (,mM
R1548 T2431 T2429 parataxis mM,EDTA
R1549 T2432 T2431 nummod 20,mM
R1550 T2433 T2431 punct ),mM
R1551 T2434 T2429 punct ", ",EDTA
R1552 T2435 T2429 conj SDS,EDTA
R1553 T2436 T2437 punct (,%
R1554 T2437 T2435 parataxis %,SDS
R1555 T2438 T2437 nummod 0.1,%
R1556 T2439 T2437 punct ),%
R1557 T2440 T2435 cc and,SDS
R1558 T2441 T2442 nummod 2,μg
R1559 T2442 T2443 compound μg,K
R1560 T2443 T2435 conj K,SDS
R1561 T2444 T2443 compound proteinase,K
R1562 T2445 T2427 punct ", ",addition
R1563 T2446 T2427 acl followed,addition
R1564 T2447 T2446 agent by,followed
R1565 T2448 T2447 pobj incubation,by
R1566 T2449 T2448 prep for,incubation
R1567 T2450 T2451 nummod 20,min
R1568 T2451 T2449 pobj min,for
R1569 T2452 T2424 punct .,stopped
R1570 T2454 T2455 nsubjpass Aliquots,electrophoresed
R1571 T2456 T2455 auxpass were,electrophoresed
R1572 T2457 T2455 prep on,electrophoresed
R1573 T2458 T2459 det a,gel
R1574 T2459 T2457 pobj gel,on
R1575 T2460 T2461 nummod 6.5,%
R1576 T2461 T2459 compound %,gel
R1577 T2462 T2459 compound polyacrylamide,gel
R1578 T2463 T2455 prep in,electrophoresed
R1579 T2464 T2465 nummod 1,TBE
R1580 T2465 T2463 pobj TBE,in
R1581 T2466 T2464 punct ×,1
R1582 T2467 T2455 prep at,electrophoresed
R1583 T2468 T2469 nummod 300,V
R1584 T2469 T2467 pobj V,at
R1585 T2470 T2455 prep for,electrophoresed
R1586 T2471 T2472 nummod 2.5,h
R1587 T2472 T2470 pobj h,for
R1588 T2473 T2455 punct .,electrophoresed
R1589 T2475 T2476 det The,DNAs
R1590 T2476 T2481 nsubj DNAs,were
R1591 T2477 T2478 amod single,stranded
R1592 T2478 T2476 amod stranded,DNAs
R1593 T2479 T2478 punct -,stranded
R1594 T2480 T2476 amod circular,DNAs
R1595 T2482 T2476 acl used,DNAs
R1596 T2483 T2481 attr M13mp18,were
R1597 T2484 T2483 cc and,M13mp18
R1598 T2485 T2483 conj M13mp19,M13mp18
R1599 T2486 T2483 punct +,M13mp18
R1600 T2487 T2483 appos G,M13mp18
R1601 T2488 T2487 punct -,G
R1602 T2489 T2487 amod rich,G
R1603 T2490 T2481 punct .,were
R1604 T2492 T2493 det The,latter
R1605 T2493 T2494 nsubjpass latter,constructed
R1606 T2495 T2494 auxpass was,constructed
R1607 T2496 T2494 prep by,constructed
R1608 T2497 T2496 pcomp inserting,by
R1609 T2498 T2499 det a,sequence
R1610 T2499 T2497 dobj sequence,inserting
R1611 T2500 T2499 nmod G,sequence
R1612 T2501 T2500 punct -,G
R1613 T2502 T2500 amod rich,G
R1614 T2503 T2499 compound repeat,sequence
R1615 T2504 T2499 punct (,sequence
R1616 T2505 T2499 appos GGGGCGTGGGC,sequence
R1617 T2506 T2505 punct ),GGGGCGTGGGC
R1618 T2507 T2505 nummod 6,GGGGCGTGGGC
R1619 T2508 T2499 punct ", ",sequence
R1620 T2509 T2499 acl prepared,sequence
R1621 T2510 T2509 prep by,prepared
R1622 T2511 T2510 pcomp annealing,by
R1623 T2512 T2511 prep of,annealing
R1624 T2513 T2514 nummod two,oligonucleotides
R1625 T2514 T2512 pobj oligonucleotides,of
R1626 T2515 T2514 punct (,oligonucleotides
R1627 T2516 T2514 appos 5,oligonucleotides
R1628 T2517 T2516 punct ′,5
R1629 T2518 T2516 punct -,5
R1630 T2519 T2520 nmod GATCC,GGGGCGTGGGC
R1631 T2520 T2516 appos GGGGCGTGGGC,5
R1632 T2521 T2520 punct -,GGGGCGTGGGC
R1633 T2522 T2520 punct [,GGGGCGTGGGC
R1634 T2523 T2520 punct ],GGGGCGTGGGC
R1635 T2524 T2520 nummod 6,GGGGCGTGGGC
R1636 T2525 T2516 punct -,5
R1637 T2526 T2516 nummod 3,5
R1638 T2527 T2516 punct ′,5
R1639 T2528 T2516 punct /,5
R1640 T2529 T2516 appos 5,5
R1641 T2530 T2529 punct ′,5
R1642 T2531 T2529 punct -,5
R1643 T2532 T2533 nmod GATCC,GCCCACGCCCC
R1644 T2533 T2529 appos GCCCACGCCCC,5
R1645 T2534 T2533 punct -,GCCCACGCCCC
R1646 T2535 T2533 punct [,GCCCACGCCCC
R1647 T2536 T2533 punct ],GCCCACGCCCC
R1648 T2537 T2533 nummod 6,GCCCACGCCCC
R1649 T2538 T2529 punct -,5
R1650 T2539 T2529 nummod 3,5
R1651 T2540 T2529 punct ′,5
R1652 T2541 T2514 punct ),oligonucleotides
R1653 T2542 T2497 prep at,inserting
R1654 T2543 T2544 det the,site
R1655 T2544 T2542 pobj site,at
R1656 T2545 T2544 compound BamHI,site
R1657 T2546 T2544 prep of,site
R1658 T2547 T2546 pobj M13mp19,of
R1659 T2548 T2494 cc and,constructed
R1660 T2549 T2550 det the,sequences
R1661 T2550 T2551 nsubjpass sequences,confirmed
R1662 T2551 T2494 conj confirmed,constructed
R1663 T2552 T2550 prep of,sequences
R1664 T2553 T2554 det the,insert
R1665 T2554 T2552 pobj insert,of
R1666 T2555 T2551 auxpass were,confirmed
R1667 T2556 T2551 punct .,confirmed

craft-ca-core-ex-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T1897 0-3 CHEBI_SO_EXT:DNA denotes DNA
T1898 19-28 SO_EXT:biological_sequence denotes sequences
T1899 41-57 CHEBI_SO_EXT:oligonucleotide denotes oligonucleotides
T1900 84-87 CHEBI_SO_EXT:DNA denotes DNA
T1901 147-153 SO:0000985 denotes duplex
T1902 185-194 GO:0097617 denotes annealing
T1903 195-202 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes labeled
T1904 203-219 CHEBI_SO_EXT:oligonucleotide denotes oligonucleotides
T1905 233-248 _FRAGMENT denotes single-stranded
T1906 258-261 CHEBI_SO_EXT:single_stranded_DNA denotes DNA
T1907 249-257 SO:0000988 denotes circular
T1908 285-292 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes labeled
T1909 392-395 CHEBI_SO_EXT:DNA denotes DNA
T1910 462-478 CHEBI_SO_EXT:oligonucleotide denotes oligonucleotides
T1911 506-515 SO_EXT:biological_sequence denotes sequences
T1912 538-542 GO:0005657 denotes fork
T1913 571-580 GO:0097617 denotes annealing
T1914 585-601 CHEBI_SO_EXT:oligonucleotide denotes oligonucleotides
T1915 652-656 GO:0005657 denotes fork
T1916 661-664 _FRAGMENT denotes 3′-
T1917 671-680 GO:0006272 denotes extension
T1918 668-680 GO:0006273 denotes 5′-extension
T1919 717-733 CHEBI_SO_EXT:oligonucleotide denotes oligonucleotides
T1920 756-764 GO:0097617 denotes annealed
T1921 773-781 PR_EXT:000009861 denotes lamin B2
T1922 797-798 CHEBI_EXT:thymine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group denotes T
T1923 818-826 PR_EXT:000009861 denotes lamin B2
T1924 827-833 SO_EXT:0000296 denotes origin
T1925 909-914 PR_EXT:000000084 denotes c-myc
T1926 922-927 PR_EXT:000000084 denotes c-myc
T1927 944-953 SO_EXT:biological_sequence denotes sequences
T1928 993-1004 CHEBI_SO_EXT:nucleotide denotes nucleotides
T1929 1094-1100 GO:0032508 denotes melted
T1930 1129-1136 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes labeled
T1931 1137-1152 CHEBI_SO_EXT:oligonucleotide denotes oligonucleotide
T1932 1166-1174 GO:0097617 denotes annealed
T1933 1186-1193 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes labeled
T1934 1194-1209 CHEBI_SO_EXT:oligonucleotide denotes oligonucleotide
T1935 1233-1240 CHEBI:60004 denotes mixture
T1936 1266-1270 CHEBI:9754 denotes Tris
T1937 1271-1274 CHEBI:17883 denotes HCl
T1938 1291-1296 CHEBI:6636 denotes MgCl2
T1939 1307-1311 CHEBI:26710 denotes NaCl
T1940 1452-1466 CHEBI_EXT:polyacrylamide denotes polyacrylamide
T1941 1490-1496 CHEBI_CHMO_EXT:buffer_solution denotes buffer
T1942 1521-1527 SO:0000985 denotes duplex
T1943 1544-1559 _FRAGMENT denotes single-stranded
T1944 1569-1572 CHEBI_SO_EXT:single_stranded_DNA denotes DNA
T1945 1560-1568 SO:0000988 denotes circular
T1946 1596-1602 SO:0000985 denotes duplex
T1947 1638-1641 CHEBI_SO_EXT:DNA denotes DNA
T1948 1697-1708 GO:0097617 denotes hybridizing
T1949 1743-1749 SO_EXT:0000440 denotes vector
T1950 1751-1759 GO:0097617 denotes annealed
T1951 1767-1782 _FRAGMENT denotes single-stranded
T1952 1792-1795 CHEBI_SO_EXT:single_stranded_DNA denotes DNA
T1953 1783-1791 SO:0000988 denotes circular
T1954 1797-1800 CHEBI_SO_EXT:DNA denotes DNA
T1955 1860-1863 CHEBI:37972 denotes 32P
T1956 1864-1868 CHEBI_EXT:dGTP denotes dGTP
T1957 1929-1934 CHEBI_EXT:dNTP denotes dNTPs
T1958 1971-1978 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes labeled
T1959 1979-1985 SO:0000985 denotes duplex
T1960 2090-2094 PR_EXT:000010246 denotes Mcm4
T1961 2090-2106 GO:0097373 denotes Mcm4/6/7 complex
T1962 2135-2142 CHEBI:60004 denotes mixture
T1963 2209-2213 CHEBI_EXT:EDTA denotes EDTA
T1964 2223-2226 CHEBI:8984 denotes SDS
T1965 2243-2253 GO_EXT:0008233 denotes proteinase
T1966 2332-2346 CHEBI_EXT:polyacrylamide denotes polyacrylamide
T1967 2385-2400 _FRAGMENT denotes single-stranded
T1968 2410-2414 CHEBI_SO_EXT:single_stranded_DNA denotes DNAs
T1969 2401-2409 SO:0000988 denotes circular
T1970 2445-2446 CHEBI_EXT:guanine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group denotes G
T1971 2483-2492 SO_EXT:sequence_insertion_process denotes inserting
T1972 2495-2496 CHEBI_EXT:guanine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group denotes G
T1973 2502-2508 SO_EXT:sequence_repeat_unit_or_region denotes repeat
T1974 2509-2517 SO_EXT:biological_sequence denotes sequence
T1975 2546-2555 GO:0097617 denotes annealing
T1976 2563-2579 CHEBI_SO_EXT:oligonucleotide denotes oligonucleotides
T1977 2643-2648 PR_EXT:P23940 denotes BamHI
T1978 2673-2682 SO_EXT:biological_sequence denotes sequences
T1979 2690-2696 SO_EXT:0000667 denotes insert
R1119 T1906 T1905 _lexicallyChainedTo DNA,single-stranded
R1120 T1917 T1916 _lexicallyChainedTo extension,3′-
R1121 T1944 T1943 _lexicallyChainedTo DNA,single-stranded
R1122 T1952 T1951 _lexicallyChainedTo DNA,single-stranded
R1123 T1968 T1967 _lexicallyChainedTo DNAs,single-stranded

craft-ca-core-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T1854 147-153 SO:0000985 denotes duplex
T1855 185-194 GO:0097617 denotes annealing
T1856 233-248 SO:0000984 denotes single-stranded
T1857 249-257 SO:0000988 denotes circular
T1858 538-542 GO:0005657 denotes fork
T1859 571-580 GO:0097617 denotes annealing
T1860 652-656 GO:0005657 denotes fork
T1861 661-664 _FRAGMENT denotes 3′-
T1862 671-680 GO:0006272 denotes extension
T1863 668-680 GO:0006273 denotes 5′-extension
T1864 756-764 GO:0097617 denotes annealed
T1865 773-781 PR:000009861 denotes lamin B2
T1866 818-826 PR:000009861 denotes lamin B2
T1867 827-833 SO:0000296 denotes origin
T1868 909-914 PR:000000084 denotes c-myc
T1869 922-927 PR:000000084 denotes c-myc
T1870 1094-1100 GO:0032508 denotes melted
T1871 1166-1174 GO:0097617 denotes annealed
T1872 1233-1240 CHEBI:60004 denotes mixture
T1873 1266-1270 CHEBI:9754 denotes Tris
T1874 1271-1274 CHEBI:17883 denotes HCl
T1875 1291-1296 CHEBI:6636 denotes MgCl2
T1876 1307-1311 CHEBI:26710 denotes NaCl
T1877 1521-1527 SO:0000985 denotes duplex
T1878 1544-1559 SO:0000984 denotes single-stranded
T1879 1560-1568 SO:0000988 denotes circular
T1880 1596-1602 SO:0000985 denotes duplex
T1881 1697-1708 GO:0097617 denotes hybridizing
T1882 1743-1749 SO:0000440 denotes vector
T1883 1751-1759 GO:0097617 denotes annealed
T1884 1767-1782 SO:0000984 denotes single-stranded
T1885 1783-1791 SO:0000988 denotes circular
T1886 1860-1863 CHEBI:37972 denotes 32P
T1887 1979-1985 SO:0000985 denotes duplex
T1888 2090-2094 PR:000010246 denotes Mcm4
T1889 2090-2106 GO:0097373 denotes Mcm4/6/7 complex
T1890 2135-2142 CHEBI:60004 denotes mixture
T1891 2223-2226 CHEBI:8984 denotes SDS
T1892 2385-2400 SO:0000984 denotes single-stranded
T1893 2401-2409 SO:0000988 denotes circular
T1894 2546-2555 GO:0097617 denotes annealing
T1895 2643-2648 PR:P23940 denotes BamHI
T1896 2690-2696 SO:0000667 denotes insert
R1118 T1862 T1861 _lexicallyChainedTo extension,3′-

2_test

Id Subject Object Predicate Lexical cue
15917436-14609960-76775505 882-884 14609960 denotes 13
15917436-14609960-76775506 2013-2015 14609960 denotes 13
15917436-10567526-76775507 2157-2159 10567526 denotes 11