Id |
Subject |
Object |
Predicate |
Lexical cue |
T1450 |
0-7 |
VBN |
denotes |
Labeled |
T1451 |
28-33 |
NNS |
denotes |
rNTPs |
T1452 |
8-11 |
CC |
denotes |
and |
T1453 |
12-21 |
JJ |
denotes |
unlabeled |
T1454 |
22-27 |
NNS |
denotes |
dNTPs |
T1455 |
27-28 |
HYPH |
denotes |
/ |
T1456 |
53-62 |
VBN |
denotes |
purchased |
T1457 |
34-37 |
CC |
denotes |
and |
T1458 |
38-47 |
NN |
denotes |
Sequenase |
T1459 |
48-52 |
VBD |
denotes |
were |
T1460 |
63-67 |
IN |
denotes |
from |
T1461 |
68-76 |
NNP |
denotes |
Amersham |
T1462 |
77-86 |
NNP |
denotes |
Pharmacia |
T1463 |
86-87 |
. |
denotes |
. |
T1464 |
87-314 |
sentence |
denotes |
M13mp18 and M13mp19 single-stranded circular DNA, T4 polynucleotide kinase, DNase I and the Klenow fragment were from TAKARA, Nuclease P1 was from Roche, and anti-FLAG M2 Ab (antibody)-agarose and FLAG peptide were from Sigma. |
T1465 |
88-95 |
NN |
denotes |
M13mp18 |
T1466 |
133-136 |
NN |
denotes |
DNA |
T1467 |
96-99 |
CC |
denotes |
and |
T1468 |
100-107 |
NN |
denotes |
M13mp19 |
T1469 |
108-114 |
JJ |
denotes |
single |
T1470 |
115-123 |
VBN |
denotes |
stranded |
T1471 |
114-115 |
HYPH |
denotes |
- |
T1472 |
124-132 |
JJ |
denotes |
circular |
T1473 |
196-200 |
VBD |
denotes |
were |
T1474 |
136-138 |
, |
denotes |
, |
T1475 |
138-140 |
NN |
denotes |
T4 |
T1476 |
156-162 |
NN |
denotes |
kinase |
T1477 |
141-155 |
NN |
denotes |
polynucleotide |
T1478 |
162-164 |
, |
denotes |
, |
T1479 |
164-169 |
NN |
denotes |
DNase |
T1480 |
170-171 |
CD |
denotes |
I |
T1481 |
172-175 |
CC |
denotes |
and |
T1482 |
176-179 |
DT |
denotes |
the |
T1483 |
187-195 |
NN |
denotes |
fragment |
T1484 |
180-186 |
NN |
denotes |
Klenow |
T1485 |
201-205 |
IN |
denotes |
from |
T1486 |
206-212 |
NN |
denotes |
TAKARA |
T1487 |
212-214 |
, |
denotes |
, |
T1488 |
214-222 |
NN |
denotes |
Nuclease |
T1489 |
223-225 |
NN |
denotes |
P1 |
T1490 |
226-229 |
VBD |
denotes |
was |
T1491 |
230-234 |
IN |
denotes |
from |
T1492 |
235-240 |
NNP |
denotes |
Roche |
T1493 |
240-242 |
, |
denotes |
, |
T1494 |
242-245 |
CC |
denotes |
and |
T1495 |
246-255 |
JJ |
denotes |
anti-FLAG |
T1496 |
273-280 |
NN |
denotes |
agarose |
T1497 |
256-258 |
NN |
denotes |
M2 |
T1498 |
259-261 |
NN |
denotes |
Ab |
T1499 |
263-271 |
NN |
denotes |
antibody |
T1500 |
262-263 |
-LRB- |
denotes |
( |
T1501 |
271-272 |
-RRB- |
denotes |
) |
T1502 |
272-273 |
HYPH |
denotes |
- |
T1503 |
298-302 |
VBD |
denotes |
were |
T1504 |
281-284 |
CC |
denotes |
and |
T1505 |
285-289 |
NN |
denotes |
FLAG |
T1506 |
290-297 |
NN |
denotes |
peptide |
T1507 |
303-307 |
IN |
denotes |
from |
T1508 |
308-313 |
NNP |
denotes |
Sigma |
T1509 |
313-314 |
. |
denotes |
. |
T1510 |
314-399 |
sentence |
denotes |
SV40 T-antigen was purified from baculovirus-infected insect cells as reported (22). |
T1511 |
315-319 |
NN |
denotes |
SV40 |
T1512 |
322-329 |
NN |
denotes |
antigen |
T1513 |
320-321 |
NN |
denotes |
T |
T1514 |
321-322 |
HYPH |
denotes |
- |
T1515 |
334-342 |
VBN |
denotes |
purified |
T1516 |
330-333 |
VBD |
denotes |
was |
T1517 |
343-347 |
IN |
denotes |
from |
T1518 |
348-359 |
NN |
denotes |
baculovirus |
T1519 |
360-368 |
VBN |
denotes |
infected |
T1520 |
359-360 |
HYPH |
denotes |
- |
T1521 |
376-381 |
NNS |
denotes |
cells |
T1522 |
369-375 |
NN |
denotes |
insect |
T1523 |
382-384 |
IN |
denotes |
as |
T1524 |
385-393 |
VBN |
denotes |
reported |
T1525 |
394-395 |
-LRB- |
denotes |
( |
T1526 |
395-397 |
CD |
denotes |
22 |
T1527 |
397-398 |
-RRB- |
denotes |
) |
T1528 |
398-399 |
. |
denotes |
. |
T1529 |
399-458 |
sentence |
denotes |
PriA helicase protein was provided by Dr Taku Tanaka (23). |
T1530 |
400-404 |
NN |
denotes |
PriA |
T1531 |
405-413 |
NN |
denotes |
helicase |
T1532 |
414-421 |
NN |
denotes |
protein |
T1533 |
426-434 |
VBN |
denotes |
provided |
T1534 |
422-425 |
VBD |
denotes |
was |
T1535 |
435-437 |
IN |
denotes |
by |
T1536 |
438-440 |
NNP |
denotes |
Dr |
T1537 |
446-452 |
NNP |
denotes |
Tanaka |
T1538 |
441-445 |
NNP |
denotes |
Taku |
T1539 |
453-454 |
-LRB- |
denotes |
( |
T1540 |
454-456 |
CD |
denotes |
23 |
T1541 |
456-457 |
-RRB- |
denotes |
) |
T1542 |
457-458 |
. |
denotes |
. |
T1543 |
458-660 |
sentence |
denotes |
Oligonucleotides were purchased from Hokkaido system science Co., Ltd (Hokkaido, Japan), and were purified on polyacrylamide gel, followed by purification by Sep-Pak Plus C18 cartridge column (Waters). |
T1544 |
459-475 |
NNS |
denotes |
Oligonucleotides |
T1545 |
481-490 |
VBN |
denotes |
purchased |
T1546 |
476-480 |
VBD |
denotes |
were |
T1547 |
491-495 |
IN |
denotes |
from |
T1548 |
496-504 |
NNP |
denotes |
Hokkaido |
T1549 |
525-528 |
NNP |
denotes |
Ltd |
T1550 |
505-511 |
NNP |
denotes |
system |
T1551 |
512-519 |
NNP |
denotes |
science |
T1552 |
520-523 |
NNP |
denotes |
Co. |
T1553 |
523-525 |
, |
denotes |
, |
T1554 |
529-530 |
-LRB- |
denotes |
( |
T1555 |
530-538 |
NNP |
denotes |
Hokkaido |
T1556 |
538-540 |
, |
denotes |
, |
T1557 |
540-545 |
NNP |
denotes |
Japan |
T1558 |
545-546 |
-RRB- |
denotes |
) |
T1559 |
546-548 |
, |
denotes |
, |
T1560 |
548-551 |
CC |
denotes |
and |
T1561 |
552-556 |
VBD |
denotes |
were |
T1562 |
557-565 |
VBN |
denotes |
purified |
T1563 |
566-568 |
IN |
denotes |
on |
T1564 |
569-583 |
NN |
denotes |
polyacrylamide |
T1565 |
584-587 |
NN |
denotes |
gel |
T1566 |
587-589 |
, |
denotes |
, |
T1567 |
589-597 |
VBN |
denotes |
followed |
T1568 |
598-600 |
IN |
denotes |
by |
T1569 |
601-613 |
NN |
denotes |
purification |
T1570 |
614-616 |
IN |
denotes |
by |
T1571 |
617-620 |
NN |
denotes |
Sep |
T1572 |
625-629 |
NN |
denotes |
Plus |
T1573 |
620-621 |
HYPH |
denotes |
- |
T1574 |
621-624 |
NN |
denotes |
Pak |
T1575 |
634-643 |
NN |
denotes |
cartridge |
T1576 |
630-633 |
NN |
denotes |
C18 |
T1577 |
644-650 |
NN |
denotes |
column |
T1578 |
651-652 |
-LRB- |
denotes |
( |
T1579 |
652-658 |
NNP |
denotes |
Waters |
T1580 |
658-659 |
-RRB- |
denotes |
) |
T1581 |
659-660 |
. |
denotes |
. |
R872 |
T1450 |
T1451 |
amod |
Labeled,rNTPs |
R873 |
T1451 |
T1456 |
nsubjpass |
rNTPs,purchased |
R874 |
T1452 |
T1450 |
cc |
and,Labeled |
R875 |
T1453 |
T1450 |
conj |
unlabeled,Labeled |
R876 |
T1454 |
T1451 |
compound |
dNTPs,rNTPs |
R877 |
T1455 |
T1451 |
punct |
/,rNTPs |
R878 |
T1457 |
T1451 |
cc |
and,rNTPs |
R879 |
T1458 |
T1451 |
conj |
Sequenase,rNTPs |
R880 |
T1459 |
T1456 |
auxpass |
were,purchased |
R881 |
T1460 |
T1456 |
prep |
from,purchased |
R882 |
T1461 |
T1462 |
compound |
Amersham,Pharmacia |
R883 |
T1462 |
T1460 |
pobj |
Pharmacia,from |
R884 |
T1463 |
T1456 |
punct |
.,purchased |
R885 |
T1465 |
T1466 |
nmod |
M13mp18,DNA |
R886 |
T1466 |
T1473 |
nsubj |
DNA,were |
R887 |
T1467 |
T1465 |
cc |
and,M13mp18 |
R888 |
T1468 |
T1465 |
conj |
M13mp19,M13mp18 |
R889 |
T1469 |
T1470 |
amod |
single,stranded |
R890 |
T1470 |
T1466 |
amod |
stranded,DNA |
R891 |
T1471 |
T1470 |
punct |
-,stranded |
R892 |
T1472 |
T1466 |
amod |
circular,DNA |
R893 |
T1474 |
T1466 |
punct |
", ",DNA |
R894 |
T1475 |
T1476 |
compound |
T4,kinase |
R895 |
T1476 |
T1466 |
conj |
kinase,DNA |
R896 |
T1477 |
T1476 |
compound |
polynucleotide,kinase |
R897 |
T1478 |
T1476 |
punct |
", ",kinase |
R898 |
T1479 |
T1476 |
conj |
DNase,kinase |
R899 |
T1480 |
T1479 |
nummod |
I,DNase |
R900 |
T1481 |
T1479 |
cc |
and,DNase |
R901 |
T1482 |
T1483 |
det |
the,fragment |
R902 |
T1483 |
T1479 |
conj |
fragment,DNase |
R903 |
T1484 |
T1483 |
compound |
Klenow,fragment |
R904 |
T1485 |
T1473 |
prep |
from,were |
R905 |
T1486 |
T1485 |
pobj |
TAKARA,from |
R906 |
T1487 |
T1473 |
punct |
", ",were |
R907 |
T1488 |
T1489 |
compound |
Nuclease,P1 |
R908 |
T1489 |
T1490 |
nsubj |
P1,was |
R909 |
T1490 |
T1473 |
conj |
was,were |
R910 |
T1491 |
T1490 |
prep |
from,was |
R911 |
T1492 |
T1491 |
pobj |
Roche,from |
R912 |
T1493 |
T1490 |
punct |
", ",was |
R913 |
T1494 |
T1490 |
cc |
and,was |
R914 |
T1495 |
T1496 |
amod |
anti-FLAG,agarose |
R915 |
T1496 |
T1503 |
nsubj |
agarose,were |
R916 |
T1497 |
T1496 |
nmod |
M2,agarose |
R917 |
T1498 |
T1499 |
nmod |
Ab,antibody |
R918 |
T1499 |
T1496 |
nmod |
antibody,agarose |
R919 |
T1500 |
T1499 |
punct |
(,antibody |
R920 |
T1501 |
T1496 |
punct |
),agarose |
R921 |
T1502 |
T1496 |
punct |
-,agarose |
R922 |
T1503 |
T1490 |
conj |
were,was |
R923 |
T1504 |
T1496 |
cc |
and,agarose |
R924 |
T1505 |
T1506 |
compound |
FLAG,peptide |
R925 |
T1506 |
T1496 |
conj |
peptide,agarose |
R926 |
T1507 |
T1503 |
prep |
from,were |
R927 |
T1508 |
T1507 |
pobj |
Sigma,from |
R928 |
T1509 |
T1503 |
punct |
.,were |
R929 |
T1511 |
T1512 |
compound |
SV40,antigen |
R930 |
T1512 |
T1515 |
nsubjpass |
antigen,purified |
R931 |
T1513 |
T1512 |
compound |
T,antigen |
R932 |
T1514 |
T1512 |
punct |
-,antigen |
R933 |
T1516 |
T1515 |
auxpass |
was,purified |
R934 |
T1517 |
T1515 |
prep |
from,purified |
R935 |
T1518 |
T1519 |
npadvmod |
baculovirus,infected |
R936 |
T1519 |
T1521 |
amod |
infected,cells |
R937 |
T1520 |
T1519 |
punct |
-,infected |
R938 |
T1521 |
T1517 |
pobj |
cells,from |
R939 |
T1522 |
T1521 |
compound |
insect,cells |
R940 |
T1523 |
T1524 |
mark |
as,reported |
R941 |
T1524 |
T1515 |
advcl |
reported,purified |
R942 |
T1525 |
T1526 |
punct |
(,22 |
R943 |
T1526 |
T1524 |
parataxis |
22,reported |
R944 |
T1527 |
T1526 |
punct |
),22 |
R945 |
T1528 |
T1515 |
punct |
.,purified |
R946 |
T1530 |
T1531 |
compound |
PriA,helicase |
R947 |
T1531 |
T1532 |
compound |
helicase,protein |
R948 |
T1532 |
T1533 |
nsubjpass |
protein,provided |
R949 |
T1534 |
T1533 |
auxpass |
was,provided |
R950 |
T1535 |
T1533 |
agent |
by,provided |
R951 |
T1536 |
T1537 |
compound |
Dr,Tanaka |
R952 |
T1537 |
T1535 |
pobj |
Tanaka,by |
R953 |
T1538 |
T1537 |
compound |
Taku,Tanaka |
R954 |
T1539 |
T1540 |
punct |
(,23 |
R955 |
T1540 |
T1533 |
parataxis |
23,provided |
R956 |
T1541 |
T1540 |
punct |
),23 |
R957 |
T1542 |
T1533 |
punct |
.,provided |
R958 |
T1544 |
T1545 |
nsubjpass |
Oligonucleotides,purchased |
R959 |
T1546 |
T1545 |
auxpass |
were,purchased |
R960 |
T1547 |
T1545 |
prep |
from,purchased |
R961 |
T1548 |
T1549 |
nmod |
Hokkaido,Ltd |
R962 |
T1549 |
T1547 |
pobj |
Ltd,from |
R963 |
T1550 |
T1549 |
nmod |
system,Ltd |
R964 |
T1551 |
T1549 |
nmod |
science,Ltd |
R965 |
T1552 |
T1549 |
nmod |
Co.,Ltd |
R966 |
T1553 |
T1549 |
punct |
", ",Ltd |
R967 |
T1554 |
T1555 |
punct |
(,Hokkaido |
R968 |
T1555 |
T1549 |
parataxis |
Hokkaido,Ltd |
R969 |
T1556 |
T1555 |
punct |
", ",Hokkaido |
R970 |
T1557 |
T1555 |
npadvmod |
Japan,Hokkaido |
R971 |
T1558 |
T1555 |
punct |
),Hokkaido |
R972 |
T1559 |
T1545 |
punct |
", ",purchased |
R973 |
T1560 |
T1545 |
cc |
and,purchased |
R974 |
T1561 |
T1562 |
auxpass |
were,purified |
R975 |
T1562 |
T1545 |
conj |
purified,purchased |
R976 |
T1563 |
T1562 |
prep |
on,purified |
R977 |
T1564 |
T1565 |
compound |
polyacrylamide,gel |
R978 |
T1565 |
T1563 |
pobj |
gel,on |
R979 |
T1566 |
T1562 |
punct |
", ",purified |
R980 |
T1567 |
T1562 |
advcl |
followed,purified |
R981 |
T1568 |
T1567 |
agent |
by,followed |
R982 |
T1569 |
T1568 |
pobj |
purification,by |
R983 |
T1570 |
T1569 |
prep |
by,purification |
R984 |
T1571 |
T1572 |
compound |
Sep,Plus |
R985 |
T1572 |
T1575 |
compound |
Plus,cartridge |
R986 |
T1573 |
T1572 |
punct |
-,Plus |
R987 |
T1574 |
T1572 |
compound |
Pak,Plus |
R988 |
T1575 |
T1577 |
compound |
cartridge,column |
R989 |
T1576 |
T1575 |
compound |
C18,cartridge |
R990 |
T1577 |
T1570 |
pobj |
column,by |
R991 |
T1578 |
T1579 |
punct |
(,Waters |
R992 |
T1579 |
T1577 |
parataxis |
Waters,column |
R993 |
T1580 |
T1579 |
punct |
),Waters |
R994 |
T1581 |
T1545 |
punct |
.,purchased |