PMC:1140370 / 4609-10893 JSON TXT

Annnotations TAB JSON ListView MergeView

craft-sa-dev

Id	Subject	Object	Predicate	Lexical cue
T1448	23-31	NNS	denotes	Reagents
T1449	31-119	sentence	denotes	Labeled and unlabeled dNTPs/rNTPs and Sequenase were purchased from Amersham Pharmacia.
T1450	32-39	VBN	denotes	Labeled
T1451	60-65	NNS	denotes	rNTPs
T1452	40-43	CC	denotes	and
T1453	44-53	JJ	denotes	unlabeled
T1454	54-59	NNS	denotes	dNTPs
T1455	59-60	HYPH	denotes	/
T1456	85-94	VBN	denotes	purchased
T1457	66-69	CC	denotes	and
T1458	70-79	NN	denotes	Sequenase
T1459	80-84	VBD	denotes	were
T1460	95-99	IN	denotes	from
T1461	100-108	NNP	denotes	Amersham
T1462	109-118	NNP	denotes	Pharmacia
T1463	118-119	.	denotes	.
T1464	119-346	sentence	denotes	M13mp18 and M13mp19 single-stranded circular DNA, T4 polynucleotide kinase, DNase I and the Klenow fragment were from TAKARA, Nuclease P1 was from Roche, and anti-FLAG M2 Ab (antibody)-agarose and FLAG peptide were from Sigma.
T1465	120-127	NN	denotes	M13mp18
T1466	165-168	NN	denotes	DNA
T1467	128-131	CC	denotes	and
T1468	132-139	NN	denotes	M13mp19
T1469	140-146	JJ	denotes	single
T1470	147-155	VBN	denotes	stranded
T1471	146-147	HYPH	denotes	-
T1472	156-164	JJ	denotes	circular
T1473	228-232	VBD	denotes	were
T1474	168-170	,	denotes	,
T1475	170-172	NN	denotes	T4
T1476	188-194	NN	denotes	kinase
T1477	173-187	NN	denotes	polynucleotide
T1478	194-196	,	denotes	,
T1479	196-201	NN	denotes	DNase
T1480	202-203	CD	denotes	I
T1481	204-207	CC	denotes	and
T1482	208-211	DT	denotes	the
T1483	219-227	NN	denotes	fragment
T1484	212-218	NN	denotes	Klenow
T1485	233-237	IN	denotes	from
T1486	238-244	NN	denotes	TAKARA
T1487	244-246	,	denotes	,
T1488	246-254	NN	denotes	Nuclease
T1489	255-257	NN	denotes	P1
T1490	258-261	VBD	denotes	was
T1491	262-266	IN	denotes	from
T1492	267-272	NNP	denotes	Roche
T1493	272-274	,	denotes	,
T1494	274-277	CC	denotes	and
T1495	278-287	JJ	denotes	anti-FLAG
T1496	305-312	NN	denotes	agarose
T1497	288-290	NN	denotes	M2
T1498	291-293	NN	denotes	Ab
T1499	295-303	NN	denotes	antibody
T1500	294-295	-LRB-	denotes	(
T1501	303-304	-RRB-	denotes	)
T1502	304-305	HYPH	denotes	-
T1503	330-334	VBD	denotes	were
T1504	313-316	CC	denotes	and
T1505	317-321	NN	denotes	FLAG
T1506	322-329	NN	denotes	peptide
T1507	335-339	IN	denotes	from
T1508	340-345	NNP	denotes	Sigma
T1509	345-346	.	denotes	.
T1510	346-431	sentence	denotes	SV40 T-antigen was purified from baculovirus-infected insect cells as reported (22).
T1511	347-351	NN	denotes	SV40
T1512	354-361	NN	denotes	antigen
T1513	352-353	NN	denotes	T
T1514	353-354	HYPH	denotes	-
T1515	366-374	VBN	denotes	purified
T1516	362-365	VBD	denotes	was
T1517	375-379	IN	denotes	from
T1518	380-391	NN	denotes	baculovirus
T1519	392-400	VBN	denotes	infected
T1520	391-392	HYPH	denotes	-
T1521	408-413	NNS	denotes	cells
T1522	401-407	NN	denotes	insect
T1523	414-416	IN	denotes	as
T1524	417-425	VBN	denotes	reported
T1525	426-427	-LRB-	denotes	(
T1526	427-429	CD	denotes	22
T1527	429-430	-RRB-	denotes	)
T1528	430-431	.	denotes	.
T1529	431-490	sentence	denotes	PriA helicase protein was provided by Dr Taku Tanaka (23).
T1530	432-436	NN	denotes	PriA
T1531	437-445	NN	denotes	helicase
T1532	446-453	NN	denotes	protein
T1533	458-466	VBN	denotes	provided
T1534	454-457	VBD	denotes	was
T1535	467-469	IN	denotes	by
T1536	470-472	NNP	denotes	Dr
T1537	478-484	NNP	denotes	Tanaka
T1538	473-477	NNP	denotes	Taku
T1539	485-486	-LRB-	denotes	(
T1540	486-488	CD	denotes	23
T1541	488-489	-RRB-	denotes	)
T1542	489-490	.	denotes	.
T1543	490-692	sentence	denotes	Oligonucleotides were purchased from Hokkaido system science Co., Ltd (Hokkaido, Japan), and were purified on polyacrylamide gel, followed by purification by Sep-Pak Plus C18 cartridge column (Waters).
T1544	491-507	NNS	denotes	Oligonucleotides
T1545	513-522	VBN	denotes	purchased
T1546	508-512	VBD	denotes	were
T1547	523-527	IN	denotes	from
T1548	528-536	NNP	denotes	Hokkaido
T1549	557-560	NNP	denotes	Ltd
T1550	537-543	NNP	denotes	system
T1551	544-551	NNP	denotes	science
T1552	552-555	NNP	denotes	Co.
T1553	555-557	,	denotes	,
T1554	561-562	-LRB-	denotes	(
T1555	562-570	NNP	denotes	Hokkaido
T1556	570-572	,	denotes	,
T1557	572-577	NNP	denotes	Japan
T1558	577-578	-RRB-	denotes	)
T1559	578-580	,	denotes	,
T1560	580-583	CC	denotes	and
T1561	584-588	VBD	denotes	were
T1562	589-597	VBN	denotes	purified
T1563	598-600	IN	denotes	on
T1564	601-615	NN	denotes	polyacrylamide
T1565	616-619	NN	denotes	gel
T1566	619-621	,	denotes	,
T1567	621-629	VBN	denotes	followed
T1568	630-632	IN	denotes	by
T1569	633-645	NN	denotes	purification
T1570	646-648	IN	denotes	by
T1571	649-652	NN	denotes	Sep
T1572	657-661	NN	denotes	Plus
T1573	652-653	HYPH	denotes	-
T1574	653-656	NN	denotes	Pak
T1575	666-675	NN	denotes	cartridge
T1576	662-665	NN	denotes	C18
T1577	676-682	NN	denotes	column
T1578	683-684	-LRB-	denotes	(
T1579	684-690	NNP	denotes	Waters
T1580	690-691	-RRB-	denotes	)
T1581	691-692	.	denotes	.
T1667	694-704	NN	denotes	Expression
T1668	705-708	CC	denotes	and
T1669	709-721	NN	denotes	purification
T1670	722-724	IN	denotes	of
T1671	725-730	NN	denotes	mouse
T1672	740-747	NN	denotes	complex
T1673	731-735	NN	denotes	Mcm4
T1674	735-736	HYPH	denotes	/
T1675	736-737	CD	denotes	6
T1676	737-738	HYPH	denotes	/
T1677	738-739	CD	denotes	7
T1678	747-857	sentence	denotes	The recombinant baculoviruses expressing His6-Mcm4/Mcm6 or Mcm7-FLAG proteins were previously described (11).
T1679	748-751	DT	denotes	The
T1680	764-777	NNS	denotes	baculoviruses
T1681	752-763	JJ	denotes	recombinant
T1682	842-851	VBN	denotes	described
T1683	778-788	VBG	denotes	expressing
T1684	789-793	NN	denotes	His6
T1685	799-803	NN	denotes	Mcm6
T1686	793-794	HYPH	denotes	-
T1687	794-798	NN	denotes	Mcm4
T1688	798-799	HYPH	denotes	/
T1689	817-825	NN	denotes	proteins
T1690	804-806	CC	denotes	or
T1691	807-811	NN	denotes	Mcm7
T1692	812-816	NN	denotes	FLAG
T1693	811-812	HYPH	denotes	-
T1694	826-830	VBD	denotes	were
T1695	831-841	RB	denotes	previously
T1696	852-853	-LRB-	denotes	(
T1697	853-855	CD	denotes	11
T1698	855-856	-RRB-	denotes	)
T1699	856-857	.	denotes	.
T1700	857-1009	sentence	denotes	Sf9 and High five insect cells were cultured at 27°C in Sf-900 II SFM (Life Technologies, Inc.) and EX-CELL 405 medium (JRH Biosciences), respectively.
T1701	858-861	NN	denotes	Sf9
T1702	883-888	NNS	denotes	cells
T1703	862-865	CC	denotes	and
T1704	866-870	JJ	denotes	High
T1705	871-875	CD	denotes	five
T1706	876-882	NN	denotes	insect
T1707	894-902	VBN	denotes	cultured
T1708	889-893	VBD	denotes	were
T1709	903-905	IN	denotes	at
T1710	906-908	CD	denotes	27
T1711	908-910	NN	denotes	°C
T1712	911-913	IN	denotes	in
T1713	914-916	NN	denotes	Sf
T1714	924-927	NN	denotes	SFM
T1715	916-917	HYPH	denotes	-
T1716	917-920	CD	denotes	900
T1717	921-923	CD	denotes	II
T1718	928-929	-LRB-	denotes	(
T1719	948-952	NNP	denotes	Inc.
T1720	929-933	NNP	denotes	Life
T1721	934-946	NNP	denotes	Technologies
T1722	946-948	,	denotes	,
T1723	952-953	-RRB-	denotes	)
T1724	954-957	CC	denotes	and
T1725	958-960	NN	denotes	EX
T1726	961-965	NN	denotes	CELL
T1727	960-961	HYPH	denotes	-
T1728	970-976	NN	denotes	medium
T1729	966-969	CD	denotes	405
T1730	977-978	-LRB-	denotes	(
T1731	982-993	NNP	denotes	Biosciences
T1732	978-981	NNP	denotes	JRH
T1733	993-994	-RRB-	denotes	)
T1734	994-996	,	denotes	,
T1735	996-1008	RB	denotes	respectively
T1736	1008-1009	.	denotes	.
T1737	1009-1231	sentence	denotes	For expression of the Mcm4/6/7 proteins, High five cells were coinfected with recombinant baculoviruses expressing the His6-Mcm4/Mcm6 proteins and those expressing the Mcm7-FLAG, and were collected at 48 h post-infection.
T1738	1010-1013	IN	denotes	For
T1739	1072-1082	VBN	denotes	coinfected
T1740	1014-1024	NN	denotes	expression
T1741	1025-1027	IN	denotes	of
T1742	1028-1031	DT	denotes	the
T1743	1041-1049	NN	denotes	proteins
T1744	1032-1036	NN	denotes	Mcm4
T1745	1036-1037	HYPH	denotes	/
T1746	1037-1038	CD	denotes	6
T1747	1038-1039	HYPH	denotes	/
T1748	1039-1040	CD	denotes	7
T1749	1049-1051	,	denotes	,
T1750	1051-1055	JJ	denotes	High
T1751	1056-1060	CD	denotes	five
T1752	1061-1066	NNS	denotes	cells
T1753	1067-1071	VBD	denotes	were
T1754	1083-1087	IN	denotes	with
T1755	1088-1099	JJ	denotes	recombinant
T1756	1100-1113	NNS	denotes	baculoviruses
T1757	1114-1124	VBG	denotes	expressing
T1758	1125-1128	DT	denotes	the
T1759	1144-1152	NN	denotes	proteins
T1760	1129-1133	NN	denotes	His6
T1761	1139-1143	NN	denotes	Mcm6
T1762	1133-1134	HYPH	denotes	-
T1763	1134-1138	NN	denotes	Mcm4
T1764	1138-1139	HYPH	denotes	/
T1765	1153-1156	CC	denotes	and
T1766	1157-1162	DT	denotes	those
T1767	1163-1173	VBG	denotes	expressing
T1768	1174-1177	DT	denotes	the
T1769	1183-1187	NN	denotes	FLAG
T1770	1178-1182	NN	denotes	Mcm7
T1771	1182-1183	HYPH	denotes	-
T1772	1187-1189	,	denotes	,
T1773	1189-1192	CC	denotes	and
T1774	1193-1197	VBD	denotes	were
T1775	1198-1207	VBN	denotes	collected
T1776	1208-1210	IN	denotes	at
T1777	1211-1213	CD	denotes	48
T1778	1214-1215	NN	denotes	h
T1779	1216-1230	RB	denotes	post-infection
T1780	1230-1231	.	denotes	.
T1781	1231-1310	sentence	denotes	The recombinant Mcm4/6/7 complexes were purified as previously described (12).
T1782	1232-1235	DT	denotes	The
T1783	1257-1266	NNS	denotes	complexes
T1784	1236-1247	JJ	denotes	recombinant
T1785	1248-1252	NN	denotes	Mcm4
T1786	1252-1253	HYPH	denotes	/
T1787	1253-1254	CD	denotes	6
T1788	1254-1255	HYPH	denotes	/
T1789	1255-1256	CD	denotes	7
T1790	1272-1280	VBN	denotes	purified
T1791	1267-1271	VBD	denotes	were
T1792	1281-1283	IN	denotes	as
T1793	1295-1304	VBN	denotes	described
T1794	1284-1294	RB	denotes	previously
T1795	1305-1306	-LRB-	denotes	(
T1796	1306-1308	CD	denotes	12
T1797	1308-1309	-RRB-	denotes	)
T1798	1309-1310	.	denotes	.
T1980	1312-1315	NN	denotes	DNA
T1981	1316-1326	NNS	denotes	substrates
T1982	1326-1440	sentence	denotes	The sequences for all the oligonucleotides used for constructions of DNA substrates are listed in Tables 1 and 2.
T1983	1327-1330	DT	denotes	The
T1984	1331-1340	NNS	denotes	sequences
T1985	1415-1421	VBN	denotes	listed
T1986	1341-1344	IN	denotes	for
T1987	1345-1348	PDT	denotes	all
T1988	1353-1369	NNS	denotes	oligonucleotides
T1989	1349-1352	DT	denotes	the
T1990	1370-1374	VBN	denotes	used
T1991	1375-1378	IN	denotes	for
T1992	1379-1392	NNS	denotes	constructions
T1993	1393-1395	IN	denotes	of
T1994	1396-1399	NN	denotes	DNA
T1995	1400-1410	NNS	denotes	substrates
T1996	1411-1414	VBP	denotes	are
T1997	1422-1424	IN	denotes	in
T1998	1425-1431	NNS	denotes	Tables
T1999	1432-1433	CD	denotes	1
T2000	1434-1437	CC	denotes	and
T2001	1438-1439	CD	denotes	2
T2002	1439-1440	.	denotes	.
T2003	1440-1592	sentence	denotes	The partial heteroduplex substrates were constructed by annealing labeled oligonucleotides with M13mp18 single-stranded circular DNA or its derivative.
T2004	1441-1444	DT	denotes	The
T2005	1466-1476	NNS	denotes	substrates
T2006	1445-1452	JJ	denotes	partial
T2007	1453-1465	NN	denotes	heteroduplex
T2008	1482-1493	VBN	denotes	constructed
T2009	1477-1481	VBD	denotes	were
T2010	1494-1496	IN	denotes	by
T2011	1497-1506	VBG	denotes	annealing
T2012	1507-1514	VBN	denotes	labeled
T2013	1515-1531	NNS	denotes	oligonucleotides
T2014	1532-1536	IN	denotes	with
T2015	1537-1544	NN	denotes	M13mp18
T2016	1570-1573	NN	denotes	DNA
T2017	1545-1551	JJ	denotes	single
T2018	1552-1560	VBN	denotes	stranded
T2019	1551-1552	HYPH	denotes	-
T2020	1561-1569	JJ	denotes	circular
T2021	1574-1576	CC	denotes	or
T2022	1577-1580	PRP$	denotes	its
T2023	1581-1591	NN	denotes	derivative
T2024	1591-1592	.	denotes	.
T2025	1592-1699	sentence	denotes	The labeled substrates were purified by Sepharose CL4B column chromatography (Amersham Pharmacia Biotech).
T2026	1593-1596	DT	denotes	The
T2027	1605-1615	NNS	denotes	substrates
T2028	1597-1604	VBN	denotes	labeled
T2029	1621-1629	VBN	denotes	purified
T2030	1616-1620	VBD	denotes	were
T2031	1630-1632	IN	denotes	by
T2032	1633-1642	NN	denotes	Sepharose
T2033	1643-1647	NN	denotes	CL4B
T2034	1655-1669	NN	denotes	chromatography
T2035	1648-1654	NN	denotes	column
T2036	1670-1671	-LRB-	denotes	(
T2037	1690-1697	NNP	denotes	Biotech
T2038	1671-1679	NNP	denotes	Amersham
T2039	1680-1689	NNP	denotes	Pharmacia
T2040	1697-1698	-RRB-	denotes	)
T2041	1698-1699	.	denotes	.
T2042	1699-1838	sentence	denotes	The DNA bubble substrates were assembled from two partially complementary oligonucleotides with top and bottom strand sequences (Table 1).
T2043	1700-1703	DT	denotes	The
T2044	1715-1725	NNS	denotes	substrates
T2045	1704-1707	NN	denotes	DNA
T2046	1708-1714	NN	denotes	bubble
T2047	1731-1740	VBN	denotes	assembled
T2048	1726-1730	VBD	denotes	were
T2049	1741-1745	IN	denotes	from
T2050	1746-1749	CD	denotes	two
T2051	1774-1790	NNS	denotes	oligonucleotides
T2052	1750-1759	RB	denotes	partially
T2053	1760-1773	JJ	denotes	complementary
T2054	1791-1795	IN	denotes	with
T2055	1796-1799	JJ	denotes	top
T2056	1818-1827	NNS	denotes	sequences
T2057	1800-1803	CC	denotes	and
T2058	1804-1810	JJ	denotes	bottom
T2059	1811-1817	NN	denotes	strand
T2060	1828-1829	-LRB-	denotes	(
T2061	1829-1834	NN	denotes	Table
T2062	1835-1836	CD	denotes	1
T2063	1836-1837	-RRB-	denotes	)
T2064	1837-1838	.	denotes	.
T2065	1838-1937	sentence	denotes	T-tailed Y-fork substrates were prepared by annealing two oligonucleotides (dT30 and dT60 series).
T2066	1839-1840	NN	denotes	T
T2067	1841-1847	VBN	denotes	tailed
T2068	1840-1841	HYPH	denotes	-
T2069	1855-1865	NNS	denotes	substrates
T2070	1848-1849	NN	denotes	Y
T2071	1850-1854	NN	denotes	fork
T2072	1849-1850	HYPH	denotes	-
T2073	1871-1879	VBN	denotes	prepared
T2074	1866-1870	VBD	denotes	were
T2075	1880-1882	IN	denotes	by
T2076	1883-1892	VBG	denotes	annealing
T2077	1893-1896	CD	denotes	two
T2078	1897-1913	NNS	denotes	oligonucleotides
T2079	1914-1915	-LRB-	denotes	(
T2080	1929-1935	NNS	denotes	series
T2081	1915-1919	NN	denotes	dT30
T2082	1920-1923	CC	denotes	and
T2083	1924-1928	NN	denotes	dT60
T2084	1935-1936	-RRB-	denotes	)
T2085	1936-1937	.	denotes	.
T2086	1937-2077	sentence	denotes	To construct the arrested fork and 3′- or 5′-extension substrates, various combinations of oligonucleotides shown in Table 1 were annealed.
T2087	1938-1940	TO	denotes	To
T2088	1941-1950	VB	denotes	construct
T2089	2068-2076	VBN	denotes	annealed
T2090	1951-1954	DT	denotes	the
T2091	1964-1968	NN	denotes	fork
T2092	1955-1963	VBN	denotes	arrested
T2093	1969-1972	CC	denotes	and
T2094	1973-1974	CD	denotes	3
T2095	1993-2003	NNS	denotes	substrates
T2096	1974-1975	SYM	denotes	′
T2097	1975-1976	HYPH	denotes	-
T2098	1977-1979	CC	denotes	or
T2099	1980-1981	CD	denotes	5
T2100	1981-1982	SYM	denotes	′
T2101	1982-1983	HYPH	denotes	-
T2102	1983-1992	NN	denotes	extension
T2103	2003-2005	,	denotes	,
T2104	2005-2012	JJ	denotes	various
T2105	2013-2025	NNS	denotes	combinations
T2106	2026-2028	IN	denotes	of
T2107	2029-2045	NNS	denotes	oligonucleotides
T2108	2046-2051	VBN	denotes	shown
T2109	2052-2054	IN	denotes	in
T2110	2055-2060	NN	denotes	Table
T2111	2061-2062	CD	denotes	1
T2112	2063-2067	VBD	denotes	were
T2113	2076-2077	.	denotes	.
T2114	2077-2198	sentence	denotes	Bub-82/lamin B2 containing the T-rich strand of the lamin B2 origin region was constructed as previously described (13).
T2115	2078-2081	NN	denotes	Bub
T2116	2091-2093	NN	denotes	B2
T2117	2081-2082	HYPH	denotes	-
T2118	2082-2084	CD	denotes	82
T2119	2084-2085	HYPH	denotes	/
T2120	2085-2090	NN	denotes	lamin
T2121	2157-2168	VBN	denotes	constructed
T2122	2094-2104	VBG	denotes	containing
T2123	2105-2108	DT	denotes	the
T2124	2116-2122	NN	denotes	strand
T2125	2109-2110	NN	denotes	T
T2126	2111-2115	JJ	denotes	rich
T2127	2110-2111	HYPH	denotes	-
T2128	2123-2125	IN	denotes	of
T2129	2126-2129	DT	denotes	the
T2130	2146-2152	NN	denotes	region
T2131	2130-2135	NN	denotes	lamin
T2132	2136-2138	NN	denotes	B2
T2133	2139-2145	NN	denotes	origin
T2134	2153-2156	VBD	denotes	was
T2135	2169-2171	IN	denotes	as
T2136	2183-2192	VBN	denotes	described
T2137	2172-2182	RB	denotes	previously
T2138	2193-2194	-LRB-	denotes	(
T2139	2194-2196	CD	denotes	13
T2140	2196-2197	-RRB-	denotes	)
T2141	2197-2198	.	denotes	.
T2142	2198-2420	sentence	denotes	The bubble substrate, c-myc/DUE or c-myc/DUE-C, contains sequences (GenBank accession number K01908) from nucleotides 722 to 853 or those from 828 to 747 (complementary strand), respectively, in the central melted region.
T2143	2199-2202	DT	denotes	The
T2144	2210-2219	NN	denotes	substrate
T2145	2203-2209	NN	denotes	bubble
T2146	2247-2255	VBZ	denotes	contains
T2147	2219-2221	,	denotes	,
T2148	2221-2222	NN	denotes	c
T2149	2223-2226	NN	denotes	myc
T2150	2222-2223	HYPH	denotes	-
T2151	2227-2230	NN	denotes	DUE
T2152	2226-2227	HYPH	denotes	/
T2153	2231-2233	CC	denotes	or
T2154	2234-2235	NN	denotes	c
T2155	2236-2239	NN	denotes	myc
T2156	2235-2236	HYPH	denotes	-
T2157	2244-2245	NN	denotes	C
T2158	2239-2240	HYPH	denotes	/
T2159	2240-2243	NN	denotes	DUE
T2160	2243-2244	HYPH	denotes	-
T2161	2245-2247	,	denotes	,
T2162	2256-2265	NNS	denotes	sequences
T2163	2266-2267	-LRB-	denotes	(
T2164	2292-2298	NN	denotes	K01908
T2165	2267-2274	NNP	denotes	GenBank
T2166	2285-2291	NN	denotes	number
T2167	2275-2284	NN	denotes	accession
T2168	2298-2299	-RRB-	denotes	)
T2169	2300-2304	IN	denotes	from
T2170	2305-2316	NNS	denotes	nucleotides
T2171	2324-2327	CD	denotes	853
T2172	2317-2320	CD	denotes	722
T2173	2321-2323	IN	denotes	to
T2174	2328-2330	CC	denotes	or
T2175	2331-2336	DT	denotes	those
T2176	2337-2341	IN	denotes	from
T2177	2342-2345	CD	denotes	828
T2178	2346-2348	IN	denotes	to
T2179	2349-2352	CD	denotes	747
T2180	2353-2354	-LRB-	denotes	(
T2181	2368-2374	NN	denotes	strand
T2182	2354-2367	JJ	denotes	complementary
T2183	2374-2375	-RRB-	denotes	)
T2184	2375-2377	,	denotes	,
T2185	2377-2389	RB	denotes	respectively
T2186	2389-2391	,	denotes	,
T2187	2391-2393	IN	denotes	in
T2188	2394-2397	DT	denotes	the
T2189	2413-2419	NN	denotes	region
T2190	2398-2405	JJ	denotes	central
T2191	2406-2412	VBN	denotes	melted
T2192	2419-2420	.	denotes	.
T2193	2420-2719	sentence	denotes	For each substrate, labeled oligonucleotide (3 pmol) was annealed with the unlabeled oligonucleotide (6 pmol) in a reaction mixture (50 μl) containing 20 mM Tris–HCl (pH 7.5), 10 mM MgCl2 and 25 mM NaCl, which were heated to 95°C, kept at 67°C for 1 h, and then allowed to slowly cool down to 37°C.
T2194	2421-2424	IN	denotes	For
T2195	2478-2486	VBN	denotes	annealed
T2196	2425-2429	DT	denotes	each
T2197	2430-2439	NN	denotes	substrate
T2198	2439-2441	,	denotes	,
T2199	2441-2448	VBN	denotes	labeled
T2200	2449-2464	NN	denotes	oligonucleotide
T2201	2465-2466	-LRB-	denotes	(
T2202	2468-2472	NN	denotes	pmol
T2203	2466-2467	CD	denotes	3
T2204	2472-2473	-RRB-	denotes	)
T2205	2474-2477	VBD	denotes	was
T2206	2487-2491	IN	denotes	with
T2207	2492-2495	DT	denotes	the
T2208	2506-2521	NN	denotes	oligonucleotide
T2209	2496-2505	JJ	denotes	unlabeled
T2210	2522-2523	-LRB-	denotes	(
T2211	2525-2529	NN	denotes	pmol
T2212	2523-2524	CD	denotes	6
T2213	2529-2530	-RRB-	denotes	)
T2214	2531-2533	IN	denotes	in
T2215	2534-2535	DT	denotes	a
T2216	2545-2552	NN	denotes	mixture
T2217	2536-2544	NN	denotes	reaction
T2218	2553-2554	-LRB-	denotes	(
T2219	2557-2559	NN	denotes	μl
T2220	2554-2556	CD	denotes	50
T2221	2559-2560	-RRB-	denotes	)
T2222	2561-2571	VBG	denotes	containing
T2223	2572-2574	CD	denotes	20
T2224	2575-2577	NN	denotes	mM
T2225	2583-2586	NN	denotes	HCl
T2226	2578-2582	NN	denotes	Tris
T2227	2582-2583	HYPH	denotes	–
T2228	2587-2588	-LRB-	denotes	(
T2229	2588-2590	NN	denotes	pH
T2230	2591-2594	CD	denotes	7.5
T2231	2594-2595	-RRB-	denotes	)
T2232	2595-2597	,	denotes	,
T2233	2597-2599	CD	denotes	10
T2234	2600-2602	NN	denotes	mM
T2235	2603-2608	NN	denotes	MgCl2
T2236	2609-2612	CC	denotes	and
T2237	2613-2615	CD	denotes	25
T2238	2616-2618	NN	denotes	mM
T2239	2619-2623	NN	denotes	NaCl
T2240	2623-2625	,	denotes	,
T2241	2625-2630	WDT	denotes	which
T2242	2636-2642	VBN	denotes	heated
T2243	2631-2635	VBD	denotes	were
T2244	2643-2645	IN	denotes	to
T2245	2646-2648	CD	denotes	95
T2246	2648-2650	NN	denotes	°C
T2247	2650-2652	,	denotes	,
T2248	2652-2656	VBD	denotes	kept
T2249	2657-2659	IN	denotes	at
T2250	2660-2662	CD	denotes	67
T2251	2662-2664	NN	denotes	°C
T2252	2665-2668	IN	denotes	for
T2253	2669-2670	CD	denotes	1
T2254	2671-2672	NN	denotes	h
T2255	2672-2674	,	denotes	,
T2256	2674-2677	CC	denotes	and
T2257	2678-2682	RB	denotes	then
T2258	2683-2690	VBN	denotes	allowed
T2259	2691-2693	TO	denotes	to
T2260	2701-2705	VB	denotes	cool
T2261	2694-2700	RB	denotes	slowly
T2262	2706-2710	RP	denotes	down
T2263	2711-2713	IN	denotes	to
T2264	2714-2716	CD	denotes	37
T2265	2716-2718	NN	denotes	°C
T2266	2718-2719	.	denotes	.
T2267	2719-2814	sentence	denotes	The assembled substrates were purified from polyacrylamide gel by elution into TE buffer (24).
T2268	2720-2723	DT	denotes	The
T2269	2734-2744	NNS	denotes	substrates
T2270	2724-2733	VBN	denotes	assembled
T2271	2750-2758	VBN	denotes	purified
T2272	2745-2749	VBD	denotes	were
T2273	2759-2763	IN	denotes	from
T2274	2764-2778	NN	denotes	polyacrylamide
T2275	2779-2782	NN	denotes	gel
T2276	2783-2785	IN	denotes	by
T2277	2786-2793	NN	denotes	elution
T2278	2794-2798	IN	denotes	into
T2279	2799-2801	NN	denotes	TE
T2280	2802-2808	NN	denotes	buffer
T2281	2809-2810	-LRB-	denotes	(
T2282	2810-2812	CD	denotes	24
T2283	2812-2813	-RRB-	denotes	)
T2284	2813-2814	.	denotes	.
T2285	2814-3108	sentence	denotes	The partial heteroduplex substrates on a single-stranded circular DNA with varied lengths of duplex regions were prepared by extending DNA chains from the 3′ end of the dT40-37mer (37mer region hybridizing at positions 6289–6326 of M13mp18 vector) annealed to the single-stranded circular DNA.
T2286	2815-2818	DT	denotes	The
T2287	2840-2850	NNS	denotes	substrates
T2288	2819-2826	JJ	denotes	partial
T2289	2827-2839	NN	denotes	heteroduplex
T2290	2928-2936	VBN	denotes	prepared
T2291	2851-2853	IN	denotes	on
T2292	2854-2855	DT	denotes	a
T2293	2881-2884	NN	denotes	DNA
T2294	2856-2862	JJ	denotes	single
T2295	2863-2871	VBN	denotes	stranded
T2296	2862-2863	HYPH	denotes	-
T2297	2872-2880	JJ	denotes	circular
T2298	2885-2889	IN	denotes	with
T2299	2890-2896	VBN	denotes	varied
T2300	2897-2904	NNS	denotes	lengths
T2301	2905-2907	IN	denotes	of
T2302	2908-2914	NN	denotes	duplex
T2303	2915-2922	NNS	denotes	regions
T2304	2923-2927	VBD	denotes	were
T2305	2937-2939	IN	denotes	by
T2306	2940-2949	VBG	denotes	extending
T2307	2950-2953	NN	denotes	DNA
T2308	2954-2960	NNS	denotes	chains
T2309	2961-2965	IN	denotes	from
T2310	2966-2969	DT	denotes	the
T2311	2973-2976	NN	denotes	end
T2312	2970-2971	CD	denotes	3
T2313	2971-2972	SYM	denotes	′
T2314	2977-2979	IN	denotes	of
T2315	2980-2983	DT	denotes	the
T2316	2989-2994	NN	denotes	37mer
T2317	2984-2988	NN	denotes	dT40
T2318	2988-2989	HYPH	denotes	-
T2319	2995-2996	-LRB-	denotes	(
T2320	2996-3001	NN	denotes	37mer
T2321	3002-3008	NN	denotes	region
T2322	3009-3020	VBG	denotes	hybridizing
T2323	3021-3023	IN	denotes	at
T2324	3024-3033	NNS	denotes	positions
T2325	3034-3038	CD	denotes	6289
T2326	3038-3039	SYM	denotes	–
T2327	3039-3043	CD	denotes	6326
T2328	3044-3046	IN	denotes	of
T2329	3047-3054	NN	denotes	M13mp18
T2330	3055-3061	NN	denotes	vector
T2331	3061-3062	-RRB-	denotes	)
T2332	3063-3071	VBN	denotes	annealed
T2333	3072-3074	IN	denotes	to
T2334	3075-3078	DT	denotes	the
T2335	3104-3107	NN	denotes	DNA
T2336	3079-3085	JJ	denotes	single
T2337	3086-3094	VBN	denotes	stranded
T2338	3085-3086	HYPH	denotes	-
T2339	3095-3103	JJ	denotes	circular
T2340	3107-3108	.	denotes	.
T2341	3108-3329	sentence	denotes	DNA chains were elongated with Sequenase in the presence of [α-32P]dGTP, followed by extension after addition of ddGTP and all four dNTPs, resulting in substrates containing labeled duplex regions of varied lengths (13).
T2342	3109-3112	NN	denotes	DNA
T2343	3113-3119	NNS	denotes	chains
T2344	3125-3134	VBN	denotes	elongated
T2345	3120-3124	VBD	denotes	were
T2346	3135-3139	IN	denotes	with
T2347	3140-3149	NN	denotes	Sequenase
T2348	3150-3152	IN	denotes	in
T2349	3153-3156	DT	denotes	the
T2350	3157-3165	NN	denotes	presence
T2351	3166-3168	IN	denotes	of
T2352	3169-3170	-LRB-	denotes	[
T2353	3176-3180	NN	denotes	dGTP
T2354	3170-3171	NN	denotes	α
T2355	3172-3175	NN	denotes	32P
T2356	3171-3172	HYPH	denotes	-
T2357	3175-3176	-RRB-	denotes	]
T2358	3180-3182	,	denotes	,
T2359	3182-3190	VBN	denotes	followed
T2360	3191-3193	IN	denotes	by
T2361	3194-3203	NN	denotes	extension
T2362	3204-3209	IN	denotes	after
T2363	3210-3218	NN	denotes	addition
T2364	3219-3221	IN	denotes	of
T2365	3222-3227	NN	denotes	ddGTP
T2366	3228-3231	CC	denotes	and
T2367	3232-3235	DT	denotes	all
T2368	3241-3246	NNS	denotes	dNTPs
T2369	3236-3240	CD	denotes	four
T2370	3246-3248	,	denotes	,
T2371	3248-3257	VBG	denotes	resulting
T2372	3258-3260	IN	denotes	in
T2373	3261-3271	NNS	denotes	substrates
T2374	3272-3282	VBG	denotes	containing
T2375	3283-3290	VBN	denotes	labeled
T2376	3298-3305	NNS	denotes	regions
T2377	3291-3297	NN	denotes	duplex
T2378	3306-3308	IN	denotes	of
T2379	3309-3315	VBN	denotes	varied
T2380	3316-3323	NNS	denotes	lengths
T2381	3324-3325	-LRB-	denotes	(
T2382	3325-3327	CD	denotes	13
T2383	3327-3328	-RRB-	denotes	)
T2384	3328-3329	.	denotes	.
T2385	3329-3603	sentence	denotes	The substrate (5 fmol) was first incubated with indicated amount of the Mcm4/6/7 complex at 37°C for 1 h in reaction mixture as described (11), and then reactions were stopped by addition of EDTA (20 mM), SDS (0.1%) and 2 μg proteinase K, followed by incubation for 20 min.
T2386	3330-3333	DT	denotes	The
T2387	3334-3343	NN	denotes	substrate
T2388	3363-3372	VBN	denotes	incubated
T2389	3344-3345	-LRB-	denotes	(
T2390	3347-3351	NN	denotes	fmol
T2391	3345-3346	CD	denotes	5
T2392	3351-3352	-RRB-	denotes	)
T2393	3353-3356	VBD	denotes	was
T2394	3357-3362	RB	denotes	first
T2395	3373-3377	IN	denotes	with
T2396	3378-3387	VBN	denotes	indicated
T2397	3388-3394	NN	denotes	amount
T2398	3395-3397	IN	denotes	of
T2399	3398-3401	DT	denotes	the
T2400	3411-3418	NN	denotes	complex
T2401	3402-3406	NN	denotes	Mcm4
T2402	3406-3407	HYPH	denotes	/
T2403	3407-3408	CD	denotes	6
T2404	3408-3409	HYPH	denotes	/
T2405	3409-3410	CD	denotes	7
T2406	3419-3421	IN	denotes	at
T2407	3422-3424	CD	denotes	37
T2408	3424-3426	NN	denotes	°C
T2409	3427-3430	IN	denotes	for
T2410	3431-3432	CD	denotes	1
T2411	3433-3434	NN	denotes	h
T2412	3435-3437	IN	denotes	in
T2413	3438-3446	NN	denotes	reaction
T2414	3447-3454	NN	denotes	mixture
T2415	3455-3457	IN	denotes	as
T2416	3458-3467	VBN	denotes	described
T2417	3468-3469	-LRB-	denotes	(
T2418	3469-3471	CD	denotes	11
T2419	3471-3472	-RRB-	denotes	)
T2420	3472-3474	,	denotes	,
T2421	3474-3477	CC	denotes	and
T2422	3478-3482	RB	denotes	then
T2423	3483-3492	NNS	denotes	reactions
T2424	3498-3505	VBN	denotes	stopped
T2425	3493-3497	VBD	denotes	were
T2426	3506-3508	IN	denotes	by
T2427	3509-3517	NN	denotes	addition
T2428	3518-3520	IN	denotes	of
T2429	3521-3525	NN	denotes	EDTA
T2430	3526-3527	-LRB-	denotes	(
T2431	3530-3532	NN	denotes	mM
T2432	3527-3529	CD	denotes	20
T2433	3532-3533	-RRB-	denotes	)
T2434	3533-3535	,	denotes	,
T2435	3535-3538	NN	denotes	SDS
T2436	3539-3540	-LRB-	denotes	(
T2437	3543-3544	NN	denotes	%
T2438	3540-3543	CD	denotes	0.1
T2439	3544-3545	-RRB-	denotes	)
T2440	3546-3549	CC	denotes	and
T2441	3550-3551	CD	denotes	2
T2442	3552-3554	NN	denotes	μg
T2443	3566-3567	NN	denotes	K
T2444	3555-3565	NN	denotes	proteinase
T2445	3567-3569	,	denotes	,
T2446	3569-3577	VBN	denotes	followed
T2447	3578-3580	IN	denotes	by
T2448	3581-3591	NN	denotes	incubation
T2449	3592-3595	IN	denotes	for
T2450	3596-3598	CD	denotes	20
T2451	3599-3602	NN	denotes	min
T2452	3602-3603	.	denotes	.
T2453	3603-3692	sentence	denotes	Aliquots were electrophoresed on a 6.5% polyacrylamide gel in 1× TBE at 300 V for 2.5 h.
T2454	3604-3612	NNS	denotes	Aliquots
T2455	3618-3633	VBN	denotes	electrophoresed
T2456	3613-3617	VBD	denotes	were
T2457	3634-3636	IN	denotes	on
T2458	3637-3638	DT	denotes	a
T2459	3659-3662	NN	denotes	gel
T2460	3639-3642	CD	denotes	6.5
T2461	3642-3643	NN	denotes	%
T2462	3644-3658	NN	denotes	polyacrylamide
T2463	3663-3665	IN	denotes	in
T2464	3666-3667	CD	denotes	1
T2465	3669-3672	NN	denotes	TBE
T2466	3667-3668	SYM	denotes	×
T2467	3673-3675	IN	denotes	at
T2468	3676-3679	CD	denotes	300
T2469	3680-3681	NN	denotes	V
T2470	3682-3685	IN	denotes	for
T2471	3686-3689	CD	denotes	2.5
T2472	3690-3691	NN	denotes	h
T2473	3691-3692	.	denotes	.
T2474	3692-3764	sentence	denotes	The single-stranded circular DNAs used were M13mp18 and M13mp19+G-rich.
T2475	3693-3696	DT	denotes	The
T2476	3722-3726	NNS	denotes	DNAs
T2477	3697-3703	JJ	denotes	single
T2478	3704-3712	VBN	denotes	stranded
T2479	3703-3704	HYPH	denotes	-
T2480	3713-3721	JJ	denotes	circular
T2481	3732-3736	VBD	denotes	were
T2482	3727-3731	VBN	denotes	used
T2483	3737-3744	NN	denotes	M13mp18
T2484	3745-3748	CC	denotes	and
T2485	3749-3756	NN	denotes	M13mp19
T2486	3756-3757	SYM	denotes	+
T2487	3757-3758	NN	denotes	G
T2488	3758-3759	HYPH	denotes	-
T2489	3759-3763	JJ	denotes	rich
T2490	3763-3764	.	denotes	.
T2491	3764-4024	sentence	denotes	The latter was constructed by inserting a G-rich repeat sequence (GGGGCGTGGGC)6, prepared by annealing of two oligonucleotides (5′-GATCC-[GGGGCGTGGGC]6-3′/5′-GATCC-[GCCCACGCCCC]6-3′) at the BamHI site of M13mp19 and the sequences of the insert were confirmed.
T2492	3765-3768	DT	denotes	The
T2493	3769-3775	JJ	denotes	latter
T2494	3780-3791	VBN	denotes	constructed
T2495	3776-3779	VBD	denotes	was
T2496	3792-3794	IN	denotes	by
T2497	3795-3804	VBG	denotes	inserting
T2498	3805-3806	DT	denotes	a
T2499	3821-3829	NN	denotes	sequence
T2500	3807-3808	NN	denotes	G
T2501	3808-3809	HYPH	denotes	-
T2502	3809-3813	JJ	denotes	rich
T2503	3814-3820	NN	denotes	repeat
T2504	3830-3831	-LRB-	denotes	(
T2505	3831-3842	NN	denotes	GGGGCGTGGGC
T2506	3842-3843	-RRB-	denotes	)
T2507	3843-3844	CD	denotes	6
T2508	3844-3846	,	denotes	,
T2509	3846-3854	VBN	denotes	prepared
T2510	3855-3857	IN	denotes	by
T2511	3858-3867	VBG	denotes	annealing
T2512	3868-3870	IN	denotes	of
T2513	3871-3874	CD	denotes	two
T2514	3875-3891	NNS	denotes	oligonucleotides
T2515	3892-3893	-LRB-	denotes	(
T2516	3893-3894	CD	denotes	5
T2517	3894-3895	SYM	denotes	′
T2518	3895-3896	HYPH	denotes	-
T2519	3896-3901	NN	denotes	GATCC
T2520	3903-3914	NN	denotes	GGGGCGTGGGC
T2521	3901-3902	HYPH	denotes	-
T2522	3902-3903	-LRB-	denotes	[
T2523	3914-3915	-RRB-	denotes	]
T2524	3915-3916	CD	denotes	6
T2525	3916-3917	HYPH	denotes	-
T2526	3917-3918	CD	denotes	3
T2527	3918-3919	SYM	denotes	′
T2528	3919-3920	HYPH	denotes	/
T2529	3920-3921	CD	denotes	5
T2530	3921-3922	SYM	denotes	′
T2531	3922-3923	HYPH	denotes	-
T2532	3923-3928	NN	denotes	GATCC
T2533	3930-3941	NN	denotes	GCCCACGCCCC
T2534	3928-3929	HYPH	denotes	-
T2535	3929-3930	-LRB-	denotes	[
T2536	3941-3942	-RRB-	denotes	]
T2537	3942-3943	CD	denotes	6
T2538	3943-3944	HYPH	denotes	-
T2539	3944-3945	CD	denotes	3
T2540	3945-3946	SYM	denotes	′
T2541	3946-3947	-RRB-	denotes	)
T2542	3948-3950	IN	denotes	at
T2543	3951-3954	DT	denotes	the
T2544	3961-3965	NN	denotes	site
T2545	3955-3960	NN	denotes	BamHI
T2546	3966-3968	IN	denotes	of
T2547	3969-3976	NN	denotes	M13mp19
T2548	3977-3980	CC	denotes	and
T2549	3981-3984	DT	denotes	the
T2550	3985-3994	NNS	denotes	sequences
T2551	4014-4023	VBN	denotes	confirmed
T2552	3995-3997	IN	denotes	of
T2553	3998-4001	DT	denotes	the
T2554	4002-4008	NN	denotes	insert
T2555	4009-4013	VBD	denotes	were
T2556	4023-4024	.	denotes	.
T2629	4026-4029	NN	denotes	DNA
T2630	4030-4038	NN	denotes	helicase
T2631	4053-4059	NNS	denotes	assays
T2632	4039-4042	CC	denotes	and
T2633	4043-4046	NN	denotes	gel
T2634	4047-4052	NN	denotes	shift
T2635	4059-4329	sentence	denotes	In gel shift assays, Mcm4/6/7 proteins were incubated at 30°C for 30 min in reaction mixtures (15 μl) containing 25 mM HEPES–NaOH (pH 7.5), 50 mM sodium acetate, 10 mM Mg(CH3COO)2, 1 mM DTT, 0.25 μg/ml BSA, 0.5 mM ATP-γ-S and labeled substrates of the amount indicated.
T2636	4060-4062	IN	denotes	In
T2637	4104-4113	VBN	denotes	incubated
T2638	4063-4066	NN	denotes	gel
T2639	4067-4072	NN	denotes	shift
T2640	4073-4079	NNS	denotes	assays
T2641	4079-4081	,	denotes	,
T2642	4081-4085	NN	denotes	Mcm4
T2643	4090-4098	NN	denotes	proteins
T2644	4085-4086	HYPH	denotes	/
T2645	4086-4087	CD	denotes	6
T2646	4087-4088	HYPH	denotes	/
T2647	4088-4089	CD	denotes	7
T2648	4099-4103	VBD	denotes	were
T2649	4114-4116	IN	denotes	at
T2650	4117-4119	CD	denotes	30
T2651	4119-4121	NN	denotes	°C
T2652	4122-4125	IN	denotes	for
T2653	4126-4128	CD	denotes	30
T2654	4129-4132	NN	denotes	min
T2655	4133-4135	IN	denotes	in
T2656	4136-4144	NN	denotes	reaction
T2657	4145-4153	NNS	denotes	mixtures
T2658	4154-4155	-LRB-	denotes	(
T2659	4158-4160	NN	denotes	μl
T2660	4155-4157	CD	denotes	15
T2661	4160-4161	-RRB-	denotes	)
T2662	4162-4172	VBG	denotes	containing
T2663	4173-4175	CD	denotes	25
T2664	4176-4178	NN	denotes	mM
T2665	4185-4189	NN	denotes	NaOH
T2666	4179-4184	NN	denotes	HEPES
T2667	4184-4185	HYPH	denotes	–
T2668	4190-4191	-LRB-	denotes	(
T2669	4191-4193	NN	denotes	pH
T2670	4194-4197	CD	denotes	7.5
T2671	4197-4198	-RRB-	denotes	)
T2672	4198-4200	,	denotes	,
T2673	4200-4202	CD	denotes	50
T2674	4203-4205	NN	denotes	mM
T2675	4213-4220	NN	denotes	acetate
T2676	4206-4212	NN	denotes	sodium
T2677	4220-4222	,	denotes	,
T2678	4222-4224	CD	denotes	10
T2679	4225-4227	NN	denotes	mM
T2680	4231-4237	NN	denotes	CH3COO
T2681	4228-4230	NN	denotes	Mg
T2682	4230-4231	-LRB-	denotes	(
T2683	4237-4238	-RRB-	denotes	)
T2684	4238-4239	CD	denotes	2
T2685	4239-4241	,	denotes	,
T2686	4241-4242	CD	denotes	1
T2687	4243-4245	NN	denotes	mM
T2688	4246-4249	NN	denotes	DTT
T2689	4249-4251	,	denotes	,
T2690	4251-4255	CD	denotes	0.25
T2691	4256-4258	NN	denotes	μg
T2692	4262-4265	NN	denotes	BSA
T2693	4258-4259	SYM	denotes	/
T2694	4259-4261	NN	denotes	ml
T2695	4265-4267	,	denotes	,
T2696	4267-4270	CD	denotes	0.5
T2697	4271-4273	NN	denotes	mM
T2698	4280-4281	NN	denotes	S
T2699	4274-4277	NN	denotes	ATP
T2700	4277-4278	HYPH	denotes	-
T2701	4278-4279	SYM	denotes	γ
T2702	4279-4280	HYPH	denotes	-
T2703	4282-4285	CC	denotes	and
T2704	4286-4293	VBN	denotes	labeled
T2705	4294-4304	NNS	denotes	substrates
T2706	4305-4307	IN	denotes	of
T2707	4308-4311	DT	denotes	the
T2708	4312-4318	NN	denotes	amount
T2709	4319-4328	VBN	denotes	indicated
T2710	4328-4329	.	denotes	.
T2711	4329-4555	sentence	denotes	After addition of 2 μl of 50% glycerol, the reaction mixtures were directly applied on a polyacrylamide gel containing 6 mM magnesium acetate and 5% glycerol in 0.5× TBE, and electrophoresis was conducted at room temperature.
T2712	4330-4335	IN	denotes	After
T2713	4406-4413	VBN	denotes	applied
T2714	4336-4344	NN	denotes	addition
T2715	4345-4347	IN	denotes	of
T2716	4348-4349	CD	denotes	2
T2717	4350-4352	NN	denotes	μl
T2718	4353-4355	IN	denotes	of
T2719	4356-4358	CD	denotes	50
T2720	4358-4359	NN	denotes	%
T2721	4360-4368	NN	denotes	glycerol
T2722	4368-4370	,	denotes	,
T2723	4370-4373	DT	denotes	the
T2724	4383-4391	NNS	denotes	mixtures
T2725	4374-4382	NN	denotes	reaction
T2726	4392-4396	VBD	denotes	were
T2727	4397-4405	RB	denotes	directly
T2728	4414-4416	IN	denotes	on
T2729	4417-4418	DT	denotes	a
T2730	4434-4437	NN	denotes	gel
T2731	4419-4433	NN	denotes	polyacrylamide
T2732	4438-4448	VBG	denotes	containing
T2733	4449-4450	CD	denotes	6
T2734	4451-4453	NN	denotes	mM
T2735	4464-4471	NN	denotes	acetate
T2736	4454-4463	NN	denotes	magnesium
T2737	4472-4475	CC	denotes	and
T2738	4476-4477	CD	denotes	5
T2739	4477-4478	NN	denotes	%
T2740	4479-4487	NN	denotes	glycerol
T2741	4488-4490	IN	denotes	in
T2742	4491-4494	CD	denotes	0.5
T2743	4496-4499	NN	denotes	TBE
T2744	4494-4495	SYM	denotes	×
T2745	4499-4501	,	denotes	,
T2746	4501-4504	CC	denotes	and
T2747	4505-4520	NN	denotes	electrophoresis
T2748	4525-4534	VBN	denotes	conducted
T2749	4521-4524	VBD	denotes	was
T2750	4535-4537	IN	denotes	at
T2751	4538-4542	NN	denotes	room
T2752	4543-4554	NN	denotes	temperature
T2753	4554-4555	.	denotes	.
T2754	4555-4773	sentence	denotes	For DNA helicase assays, the reaction mixtures of the gel shift assays, as described above, were incubated at 30°C for 15 min, and then the ATP was added at 10 mM, followed by incubation at 37°C for additional 30 min.
T2755	4556-4559	IN	denotes	For
T2756	4653-4662	VBN	denotes	incubated
T2757	4560-4563	NN	denotes	DNA
T2758	4573-4579	NNS	denotes	assays
T2759	4564-4572	NN	denotes	helicase
T2760	4579-4581	,	denotes	,
T2761	4581-4584	DT	denotes	the
T2762	4594-4602	NNS	denotes	mixtures
T2763	4585-4593	NN	denotes	reaction
T2764	4603-4605	IN	denotes	of
T2765	4606-4609	DT	denotes	the
T2766	4620-4626	NNS	denotes	assays
T2767	4610-4613	NN	denotes	gel
T2768	4614-4619	NN	denotes	shift
T2769	4626-4628	,	denotes	,
T2770	4628-4630	IN	denotes	as
T2771	4631-4640	VBN	denotes	described
T2772	4641-4646	RB	denotes	above
T2773	4646-4648	,	denotes	,
T2774	4648-4652	VBD	denotes	were
T2775	4663-4665	IN	denotes	at
T2776	4666-4668	CD	denotes	30
T2777	4668-4670	NN	denotes	°C
T2778	4671-4674	IN	denotes	for
T2779	4675-4677	CD	denotes	15
T2780	4678-4681	NN	denotes	min
T2781	4681-4683	,	denotes	,
T2782	4683-4686	CC	denotes	and
T2783	4687-4691	RB	denotes	then
T2784	4692-4695	DT	denotes	the
T2785	4696-4699	NN	denotes	ATP
T2786	4704-4709	VBN	denotes	added
T2787	4700-4703	VBD	denotes	was
T2788	4710-4712	IN	denotes	at
T2789	4713-4715	CD	denotes	10
T2790	4716-4718	NN	denotes	mM
T2791	4718-4720	,	denotes	,
T2792	4720-4728	VBN	denotes	followed
T2793	4729-4731	IN	denotes	by
T2794	4732-4742	NN	denotes	incubation
T2795	4743-4745	IN	denotes	at
T2796	4746-4748	CD	denotes	37
T2797	4748-4750	NN	denotes	°C
T2798	4751-4754	IN	denotes	for
T2799	4755-4765	JJ	denotes	additional
T2800	4769-4772	NN	denotes	min
T2801	4766-4768	CD	denotes	30
T2802	4772-4773	.	denotes	.
T2803	4773-4908	sentence	denotes	The reactions were terminated by addition of EDTA (20 mM), SDS (0.1%) and 2 μg proteinase K, and were incubated for additional 15 min.
T2804	4774-4777	DT	denotes	The
T2805	4778-4787	NNS	denotes	reactions
T2806	4793-4803	VBN	denotes	terminated
T2807	4788-4792	VBD	denotes	were
T2808	4804-4806	IN	denotes	by
T2809	4807-4815	NN	denotes	addition
T2810	4816-4818	IN	denotes	of
T2811	4819-4823	NN	denotes	EDTA
T2812	4824-4825	-LRB-	denotes	(
T2813	4828-4830	NN	denotes	mM
T2814	4825-4827	CD	denotes	20
T2815	4830-4831	-RRB-	denotes	)
T2816	4831-4833	,	denotes	,
T2817	4833-4836	NN	denotes	SDS
T2818	4837-4838	-LRB-	denotes	(
T2819	4841-4842	NN	denotes	%
T2820	4838-4841	CD	denotes	0.1
T2821	4842-4843	-RRB-	denotes	)
T2822	4844-4847	CC	denotes	and
T2823	4848-4849	CD	denotes	2
T2824	4850-4852	NN	denotes	μg
T2825	4864-4865	NN	denotes	K
T2826	4853-4863	NN	denotes	proteinase
T2827	4865-4867	,	denotes	,
T2828	4867-4870	CC	denotes	and
T2829	4871-4875	VBD	denotes	were
T2830	4876-4885	VBN	denotes	incubated
T2831	4886-4889	IN	denotes	for
T2832	4890-4900	JJ	denotes	additional
T2833	4904-4907	NN	denotes	min
T2834	4901-4903	CD	denotes	15
T2835	4907-4908	.	denotes	.
T2836	4908-5001	sentence	denotes	The samples were separated by electrophoresis on nondenaturing polyacrylamide gel in 1× TBE.
T2837	4909-4912	DT	denotes	The
T2838	4913-4920	NNS	denotes	samples
T2839	4926-4935	VBN	denotes	separated
T2840	4921-4925	VBD	denotes	were
T2841	4936-4938	IN	denotes	by
T2842	4939-4954	NN	denotes	electrophoresis
T2843	4955-4957	IN	denotes	on
T2844	4958-4971	JJ	denotes	nondenaturing
T2845	4987-4990	NN	denotes	gel
T2846	4972-4986	NN	denotes	polyacrylamide
T2847	4991-4993	IN	denotes	in
T2848	4994-4995	CD	denotes	1
T2849	4997-5000	NN	denotes	TBE
T2850	4995-4996	SYM	denotes	×
T2851	5000-5001	.	denotes	.
T2967	5003-5011	NN	denotes	Nuclease
T2968	5012-5022	NN	denotes	protection
T2969	5023-5029	NNS	denotes	assays
T2970	5029-5281	sentence	denotes	The reaction mixtures (25 μl) contained 25 mM HEPES–NaOH (pH 7.5), 50 mM sodium acetate, 10 mM Mg(CH3COO)2, 1 mM DTT, 0.25 mg/ml BSA, 0.5 mM ATP-γ-S, 0.5 mM CaCl2, 10 fmol of 32P-labeled bubble substrate, and the indicated amount of Mcm4/6/7 proteins.
T2971	5030-5033	DT	denotes	The
T2972	5043-5051	NNS	denotes	mixtures
T2973	5034-5042	NN	denotes	reaction
T2974	5060-5069	VBD	denotes	contained
T2975	5052-5053	-LRB-	denotes	(
T2976	5056-5058	NN	denotes	μl
T2977	5053-5055	CD	denotes	25
T2978	5058-5059	-RRB-	denotes	)
T2979	5070-5072	CD	denotes	25
T2980	5073-5075	NN	denotes	mM
T2981	5082-5086	NN	denotes	NaOH
T2982	5076-5081	NN	denotes	HEPES
T2983	5081-5082	HYPH	denotes	–
T2984	5087-5088	-LRB-	denotes	(
T2985	5088-5090	NN	denotes	pH
T2986	5091-5094	CD	denotes	7.5
T2987	5094-5095	-RRB-	denotes	)
T2988	5095-5097	,	denotes	,
T2989	5097-5099	CD	denotes	50
T2990	5100-5102	NN	denotes	mM
T2991	5110-5117	NN	denotes	acetate
T2992	5103-5109	NN	denotes	sodium
T2993	5117-5119	,	denotes	,
T2994	5119-5121	CD	denotes	10
T2995	5122-5124	NN	denotes	mM
T2996	5128-5134	NN	denotes	CH3COO
T2997	5125-5127	NN	denotes	Mg
T2998	5127-5128	-LRB-	denotes	(
T2999	5134-5135	-RRB-	denotes	)
T3000	5135-5136	CD	denotes	2
T3001	5136-5138	,	denotes	,
T3002	5138-5139	CD	denotes	1
T3003	5140-5142	NN	denotes	mM
T3004	5143-5146	NN	denotes	DTT
T3005	5146-5148	,	denotes	,
T3006	5148-5152	CD	denotes	0.25
T3007	5153-5155	NN	denotes	mg
T3008	5159-5162	NN	denotes	BSA
T3009	5155-5156	SYM	denotes	/
T3010	5156-5158	NN	denotes	ml
T3011	5162-5164	,	denotes	,
T3012	5164-5167	CD	denotes	0.5
T3013	5168-5170	NN	denotes	mM
T3014	5177-5178	NN	denotes	S
T3015	5171-5174	NN	denotes	ATP
T3016	5174-5175	HYPH	denotes	-
T3017	5175-5176	SYM	denotes	γ
T3018	5176-5177	HYPH	denotes	-
T3019	5178-5180	,	denotes	,
T3020	5180-5183	CD	denotes	0.5
T3021	5184-5186	NN	denotes	mM
T3022	5187-5192	NN	denotes	CaCl2
T3023	5192-5194	,	denotes	,
T3024	5194-5196	CD	denotes	10
T3025	5197-5201	NN	denotes	fmol
T3026	5202-5204	IN	denotes	of
T3027	5205-5208	NN	denotes	32P
T3028	5209-5216	VBN	denotes	labeled
T3029	5208-5209	HYPH	denotes	-
T3030	5224-5233	NN	denotes	substrate
T3031	5217-5223	NN	denotes	bubble
T3032	5233-5235	,	denotes	,
T3033	5235-5238	CC	denotes	and
T3034	5239-5242	DT	denotes	the
T3035	5253-5259	NN	denotes	amount
T3036	5243-5252	VBN	denotes	indicated
T3037	5260-5262	IN	denotes	of
T3038	5263-5267	NN	denotes	Mcm4
T3039	5272-5280	NN	denotes	proteins
T3040	5267-5268	HYPH	denotes	/
T3041	5268-5269	CD	denotes	6
T3042	5269-5270	HYPH	denotes	/
T3043	5270-5271	CD	denotes	7
T3044	5280-5281	.	denotes	.
T3045	5281-5451	sentence	denotes	After incubation at 30°C for 30 min, the indicated amount of DNase I (TAKARA Biomedical, Japan) was added, and the mixtures were incubated at room temperature for 1 min.
T3046	5282-5287	IN	denotes	After
T3047	5382-5387	VBN	denotes	added
T3048	5288-5298	NN	denotes	incubation
T3049	5299-5301	IN	denotes	at
T3050	5302-5304	CD	denotes	30
T3051	5304-5306	NN	denotes	°C
T3052	5307-5310	IN	denotes	for
T3053	5311-5313	CD	denotes	30
T3054	5314-5317	NN	denotes	min
T3055	5317-5319	,	denotes	,
T3056	5319-5322	DT	denotes	the
T3057	5333-5339	NN	denotes	amount
T3058	5323-5332	VBN	denotes	indicated
T3059	5340-5342	IN	denotes	of
T3060	5343-5348	NN	denotes	DNase
T3061	5349-5350	CD	denotes	I
T3062	5351-5352	-LRB-	denotes	(
T3063	5359-5369	NNP	denotes	Biomedical
T3064	5352-5358	NNP	denotes	TAKARA
T3065	5369-5371	,	denotes	,
T3066	5371-5376	NNP	denotes	Japan
T3067	5376-5377	-RRB-	denotes	)
T3068	5378-5381	VBD	denotes	was
T3069	5387-5389	,	denotes	,
T3070	5389-5392	CC	denotes	and
T3071	5393-5396	DT	denotes	the
T3072	5397-5405	NNS	denotes	mixtures
T3073	5411-5420	VBN	denotes	incubated
T3074	5406-5410	VBD	denotes	were
T3075	5421-5423	IN	denotes	at
T3076	5424-5428	NN	denotes	room
T3077	5429-5440	NN	denotes	temperature
T3078	5441-5444	IN	denotes	for
T3079	5445-5446	CD	denotes	1
T3080	5447-5450	NN	denotes	min
T3081	5450-5451	.	denotes	.
T3082	5451-5587	sentence	denotes	DNase I was inactivated by the addition of 250 μl of stop solution (20 mM EDTA, 0.2M sodium chloride, 1% SDS and 12.5 μg/ml yeast RNA).
T3083	5452-5457	NN	denotes	DNase
T3084	5464-5475	VBN	denotes	inactivated
T3085	5458-5459	CD	denotes	I
T3086	5460-5463	VBD	denotes	was
T3087	5476-5478	IN	denotes	by
T3088	5479-5482	DT	denotes	the
T3089	5483-5491	NN	denotes	addition
T3090	5492-5494	IN	denotes	of
T3091	5495-5498	CD	denotes	250
T3092	5499-5501	NN	denotes	μl
T3093	5502-5504	IN	denotes	of
T3094	5505-5509	NN	denotes	stop
T3095	5510-5518	NN	denotes	solution
T3096	5519-5520	-LRB-	denotes	(
T3097	5526-5530	NN	denotes	EDTA
T3098	5520-5522	CD	denotes	20
T3099	5523-5525	NN	denotes	mM
T3100	5530-5532	,	denotes	,
T3101	5532-5536	NN	denotes	0.2M
T3102	5544-5552	NN	denotes	chloride
T3103	5537-5543	NN	denotes	sodium
T3104	5552-5554	,	denotes	,
T3105	5554-5555	CD	denotes	1
T3106	5555-5556	NN	denotes	%
T3107	5557-5560	NN	denotes	SDS
T3108	5561-5564	CC	denotes	and
T3109	5565-5569	CD	denotes	12.5
T3110	5570-5572	NN	denotes	μg
T3111	5582-5585	NN	denotes	RNA
T3112	5572-5573	SYM	denotes	/
T3113	5573-5575	NN	denotes	ml
T3114	5576-5581	NN	denotes	yeast
T3115	5585-5586	-RRB-	denotes	)
T3116	5586-5587	.	denotes	.
T3117	5587-5715	sentence	denotes	For nuclease P1 footprinting assay, binding reactions were carried out under the same conditions except that CaCl2 was omitted.
T3118	5588-5591	IN	denotes	For
T3119	5647-5654	VBN	denotes	carried
T3120	5592-5600	NN	denotes	nuclease
T3121	5604-5616	NN	denotes	footprinting
T3122	5601-5603	NN	denotes	P1
T3123	5617-5622	NN	denotes	assay
T3124	5622-5624	,	denotes	,
T3125	5624-5631	NN	denotes	binding
T3126	5632-5641	NNS	denotes	reactions
T3127	5642-5646	VBD	denotes	were
T3128	5655-5658	RP	denotes	out
T3129	5659-5664	IN	denotes	under
T3130	5665-5668	DT	denotes	the
T3131	5674-5684	NNS	denotes	conditions
T3132	5669-5673	JJ	denotes	same
T3133	5685-5691	IN	denotes	except
T3134	5692-5696	IN	denotes	that
T3135	5707-5714	VBN	denotes	omitted
T3136	5697-5702	NN	denotes	CaCl2
T3137	5703-5706	VBD	denotes	was
T3138	5714-5715	.	denotes	.
T3139	5715-5800	sentence	denotes	P1 nuclease (0.3 U, Roche) was added and incubation was continued at 37°C for 1 min.
T3140	5716-5718	NN	denotes	P1
T3141	5719-5727	NN	denotes	nuclease
T3142	5747-5752	VBN	denotes	added
T3143	5728-5729	-LRB-	denotes	(
T3144	5736-5741	NNP	denotes	Roche
T3145	5729-5732	CD	denotes	0.3
T3146	5733-5734	NNP	denotes	U
T3147	5734-5736	,	denotes	,
T3148	5741-5742	-RRB-	denotes	)
T3149	5743-5746	VBD	denotes	was
T3150	5753-5756	CC	denotes	and
T3151	5757-5767	NN	denotes	incubation
T3152	5772-5781	VBN	denotes	continued
T3153	5768-5771	VBD	denotes	was
T3154	5782-5784	IN	denotes	at
T3155	5785-5787	CD	denotes	37
T3156	5787-5789	NN	denotes	°C
T3157	5790-5793	IN	denotes	for
T3158	5794-5795	CD	denotes	1
T3159	5796-5799	NN	denotes	min
T3160	5799-5800	.	denotes	.
T3161	5800-5995	sentence	denotes	Cleavage was stopped by addition of 50 μl of 25 mM EDTA and 0.4 M NaOH; the mixture was incubated at room temperature for 10 min followed by the addition of 12 μl of 3 M sodium acetate (pH 4.8).
T3162	5801-5809	NN	denotes	Cleavage
T3163	5814-5821	VBN	denotes	stopped
T3164	5810-5813	VBD	denotes	was
T3165	5889-5898	VBN	denotes	incubated
T3166	5822-5824	IN	denotes	by
T3167	5825-5833	NN	denotes	addition
T3168	5834-5836	IN	denotes	of
T3169	5837-5839	CD	denotes	50
T3170	5840-5842	NN	denotes	μl
T3171	5843-5845	IN	denotes	of
T3172	5846-5848	CD	denotes	25
T3173	5849-5851	NN	denotes	mM
T3174	5852-5856	NN	denotes	EDTA
T3175	5857-5860	CC	denotes	and
T3176	5861-5864	CD	denotes	0.4
T3177	5865-5866	NN	denotes	M
T3178	5867-5871	NN	denotes	NaOH
T3179	5871-5872	:	denotes	;
T3180	5873-5876	DT	denotes	the
T3181	5877-5884	NN	denotes	mixture
T3182	5885-5888	VBD	denotes	was
T3183	5899-5901	IN	denotes	at
T3184	5902-5906	NN	denotes	room
T3185	5907-5918	NN	denotes	temperature
T3186	5919-5922	IN	denotes	for
T3187	5923-5925	CD	denotes	10
T3188	5926-5929	NN	denotes	min
T3189	5930-5938	VBN	denotes	followed
T3190	5939-5941	IN	denotes	by
T3191	5942-5945	DT	denotes	the
T3192	5946-5954	NN	denotes	addition
T3193	5955-5957	IN	denotes	of
T3194	5958-5960	CD	denotes	12
T3195	5961-5963	NN	denotes	μl
T3196	5964-5966	IN	denotes	of
T3197	5967-5968	CD	denotes	3
T3198	5969-5970	NN	denotes	M
T3199	5978-5985	NN	denotes	acetate
T3200	5971-5977	NN	denotes	sodium
T3201	5986-5987	-LRB-	denotes	(
T3202	5987-5989	NN	denotes	pH
T3203	5990-5993	CD	denotes	4.8
T3204	5993-5994	-RRB-	denotes	)
T3205	5994-5995	.	denotes	.
T3206	5995-6134	sentence	denotes	Proteins were removed by phenol–chloroform extraction, and DNAs were collected by ethanol precipitation followed by wash with 70% ethanol.
T3207	5996-6004	NNS	denotes	Proteins
T3208	6010-6017	VBN	denotes	removed
T3209	6005-6009	VBD	denotes	were
T3210	6018-6020	IN	denotes	by
T3211	6021-6027	NN	denotes	phenol
T3212	6028-6038	NN	denotes	chloroform
T3213	6027-6028	HYPH	denotes	–
T3214	6039-6049	NN	denotes	extraction
T3215	6049-6051	,	denotes	,
T3216	6051-6054	CC	denotes	and
T3217	6055-6059	NNS	denotes	DNAs
T3218	6065-6074	VBN	denotes	collected
T3219	6060-6064	VBD	denotes	were
T3220	6075-6077	IN	denotes	by
T3221	6078-6085	NN	denotes	ethanol
T3222	6086-6099	NN	denotes	precipitation
T3223	6100-6108	VBN	denotes	followed
T3224	6109-6111	IN	denotes	by
T3225	6112-6116	NN	denotes	wash
T3226	6117-6121	IN	denotes	with
T3227	6122-6124	CD	denotes	70
T3228	6124-6125	NN	denotes	%
T3229	6126-6133	NN	denotes	ethanol
T3230	6133-6134	.	denotes	.
T3231	6134-6284	sentence	denotes	The digested DNA was subjected to electrophoresis through a 10 or 12% polyacrylamide sequencing gel containing 7 M urea, followed by autoradiography.
T3232	6135-6138	DT	denotes	The
T3233	6148-6151	NN	denotes	DNA
T3234	6139-6147	VBN	denotes	digested
T3235	6156-6165	VBN	denotes	subjected
T3236	6152-6155	VBD	denotes	was
T3237	6166-6168	IN	denotes	to
T3238	6169-6184	NN	denotes	electrophoresis
T3239	6185-6192	IN	denotes	through
T3240	6193-6194	DT	denotes	a
T3241	6231-6234	NN	denotes	gel
T3242	6195-6197	CD	denotes	10
T3243	6203-6204	NN	denotes	%
T3244	6198-6200	CC	denotes	or
T3245	6201-6203	CD	denotes	12
T3246	6205-6219	NN	denotes	polyacrylamide
T3247	6220-6230	NN	denotes	sequencing
T3248	6235-6245	VBG	denotes	containing
T3249	6246-6247	CD	denotes	7
T3250	6248-6249	NN	denotes	M
T3251	6250-6254	NN	denotes	urea
T3252	6254-6256	,	denotes	,
T3253	6256-6264	VBN	denotes	followed
T3254	6265-6267	IN	denotes	by
T3255	6268-6283	NN	denotes	autoradiography
T3256	6283-6284	.	denotes	.
R872	T1450	T1451	amod	Labeled,rNTPs
R873	T1451	T1456	nsubjpass	rNTPs,purchased
R874	T1452	T1450	cc	and,Labeled
R875	T1453	T1450	conj	unlabeled,Labeled
R876	T1454	T1451	compound	dNTPs,rNTPs
R877	T1455	T1451	punct	/,rNTPs
R878	T1457	T1451	cc	and,rNTPs
R879	T1458	T1451	conj	Sequenase,rNTPs
R880	T1459	T1456	auxpass	were,purchased
R881	T1460	T1456	prep	from,purchased
R882	T1461	T1462	compound	Amersham,Pharmacia
R883	T1462	T1460	pobj	Pharmacia,from
R884	T1463	T1456	punct	.,purchased
R885	T1465	T1466	nmod	M13mp18,DNA
R886	T1466	T1473	nsubj	DNA,were
R887	T1467	T1465	cc	and,M13mp18
R888	T1468	T1465	conj	M13mp19,M13mp18
R889	T1469	T1470	amod	single,stranded
R890	T1470	T1466	amod	stranded,DNA
R891	T1471	T1470	punct	-,stranded
R892	T1472	T1466	amod	circular,DNA
R893	T1474	T1466	punct	", ",DNA
R894	T1475	T1476	compound	T4,kinase
R895	T1476	T1466	conj	kinase,DNA
R896	T1477	T1476	compound	polynucleotide,kinase
R897	T1478	T1476	punct	", ",kinase
R898	T1479	T1476	conj	DNase,kinase
R899	T1480	T1479	nummod	I,DNase
R900	T1481	T1479	cc	and,DNase
R901	T1482	T1483	det	the,fragment
R902	T1483	T1479	conj	fragment,DNase
R903	T1484	T1483	compound	Klenow,fragment
R904	T1485	T1473	prep	from,were
R905	T1486	T1485	pobj	TAKARA,from
R906	T1487	T1473	punct	", ",were
R907	T1488	T1489	compound	Nuclease,P1
R908	T1489	T1490	nsubj	P1,was
R909	T1490	T1473	conj	was,were
R910	T1491	T1490	prep	from,was
R911	T1492	T1491	pobj	Roche,from
R912	T1493	T1490	punct	", ",was
R913	T1494	T1490	cc	and,was
R914	T1495	T1496	amod	anti-FLAG,agarose
R915	T1496	T1503	nsubj	agarose,were
R916	T1497	T1496	nmod	M2,agarose
R917	T1498	T1499	nmod	Ab,antibody
R918	T1499	T1496	nmod	antibody,agarose
R919	T1500	T1499	punct	(,antibody
R920	T1501	T1496	punct	),agarose
R921	T1502	T1496	punct	-,agarose
R922	T1503	T1490	conj	were,was
R923	T1504	T1496	cc	and,agarose
R924	T1505	T1506	compound	FLAG,peptide
R925	T1506	T1496	conj	peptide,agarose
R926	T1507	T1503	prep	from,were
R927	T1508	T1507	pobj	Sigma,from
R928	T1509	T1503	punct	.,were
R929	T1511	T1512	compound	SV40,antigen
R930	T1512	T1515	nsubjpass	antigen,purified
R931	T1513	T1512	compound	T,antigen
R932	T1514	T1512	punct	-,antigen
R933	T1516	T1515	auxpass	was,purified
R934	T1517	T1515	prep	from,purified
R935	T1518	T1519	npadvmod	baculovirus,infected
R936	T1519	T1521	amod	infected,cells
R937	T1520	T1519	punct	-,infected
R938	T1521	T1517	pobj	cells,from
R939	T1522	T1521	compound	insect,cells
R940	T1523	T1524	mark	as,reported
R941	T1524	T1515	advcl	reported,purified
R942	T1525	T1526	punct	(,22
R943	T1526	T1524	parataxis	22,reported
R944	T1527	T1526	punct	),22
R945	T1528	T1515	punct	.,purified
R946	T1530	T1531	compound	PriA,helicase
R947	T1531	T1532	compound	helicase,protein
R948	T1532	T1533	nsubjpass	protein,provided
R949	T1534	T1533	auxpass	was,provided
R950	T1535	T1533	agent	by,provided
R951	T1536	T1537	compound	Dr,Tanaka
R952	T1537	T1535	pobj	Tanaka,by
R953	T1538	T1537	compound	Taku,Tanaka
R954	T1539	T1540	punct	(,23
R955	T1540	T1533	parataxis	23,provided
R956	T1541	T1540	punct	),23
R957	T1542	T1533	punct	.,provided
R958	T1544	T1545	nsubjpass	Oligonucleotides,purchased
R959	T1546	T1545	auxpass	were,purchased
R960	T1547	T1545	prep	from,purchased
R961	T1548	T1549	nmod	Hokkaido,Ltd
R962	T1549	T1547	pobj	Ltd,from
R963	T1550	T1549	nmod	system,Ltd
R964	T1551	T1549	nmod	science,Ltd
R965	T1552	T1549	nmod	Co.,Ltd
R966	T1553	T1549	punct	", ",Ltd
R967	T1554	T1555	punct	(,Hokkaido
R968	T1555	T1549	parataxis	Hokkaido,Ltd
R969	T1556	T1555	punct	", ",Hokkaido
R970	T1557	T1555	npadvmod	Japan,Hokkaido
R971	T1558	T1555	punct	),Hokkaido
R972	T1559	T1545	punct	", ",purchased
R973	T1560	T1545	cc	and,purchased
R974	T1561	T1562	auxpass	were,purified
R975	T1562	T1545	conj	purified,purchased
R976	T1563	T1562	prep	on,purified
R977	T1564	T1565	compound	polyacrylamide,gel
R978	T1565	T1563	pobj	gel,on
R979	T1566	T1562	punct	", ",purified
R980	T1567	T1562	advcl	followed,purified
R981	T1568	T1567	agent	by,followed
R982	T1569	T1568	pobj	purification,by
R983	T1570	T1569	prep	by,purification
R984	T1571	T1572	compound	Sep,Plus
R985	T1572	T1575	compound	Plus,cartridge
R986	T1573	T1572	punct	-,Plus
R987	T1574	T1572	compound	Pak,Plus
R988	T1575	T1577	compound	cartridge,column
R989	T1576	T1575	compound	C18,cartridge
R990	T1577	T1570	pobj	column,by
R991	T1578	T1579	punct	(,Waters
R992	T1579	T1577	parataxis	Waters,column
R993	T1580	T1579	punct	),Waters
R994	T1581	T1545	punct	.,purchased
R995	T1668	T1667	cc	and,Expression
R996	T1669	T1667	conj	purification,Expression
R997	T1670	T1667	prep	of,Expression
R998	T1671	T1672	nmod	mouse,complex
R1000	T1673	T1672	nmod	Mcm4,complex
R1001	T1674	T1673	punct	/,Mcm4
R1002	T1675	T1673	nummod	6,Mcm4
R1003	T1676	T1673	punct	/,Mcm4
R1004	T1677	T1673	nummod	7,Mcm4
R1005	T1679	T1680	det	The,baculoviruses
R1006	T1680	T1682	nsubjpass	baculoviruses,described
R1007	T1681	T1680	amod	recombinant,baculoviruses
R1008	T1683	T1680	acl	expressing,baculoviruses
R1009	T1684	T1685	nmod	His6,Mcm6
R1010	T1685	T1689	nmod	Mcm6,proteins
R1011	T1686	T1685	punct	-,Mcm6
R1012	T1687	T1685	nmod	Mcm4,Mcm6
R1013	T1688	T1685	punct	/,Mcm6
R1014	T1689	T1683	dobj	proteins,expressing
R1015	T1690	T1685	cc	or,Mcm6
R1016	T1691	T1692	compound	Mcm7,FLAG
R1017	T1692	T1685	conj	FLAG,Mcm6
R1018	T1693	T1692	punct	-,FLAG
R1019	T1694	T1682	auxpass	were,described
R1020	T1695	T1682	advmod	previously,described
R1021	T1696	T1697	punct	(,11
R1022	T1697	T1682	parataxis	11,described
R1023	T1698	T1697	punct	),11
R1024	T1699	T1682	punct	.,described
R1025	T1701	T1702	nmod	Sf9,cells
R1026	T1702	T1707	nsubjpass	cells,cultured
R1027	T1703	T1701	cc	and,Sf9
R1028	T1704	T1705	amod	High,five
R1029	T1705	T1701	conj	five,Sf9
R1030	T1706	T1702	compound	insect,cells
R1031	T1708	T1707	auxpass	were,cultured
R1032	T1709	T1707	prep	at,cultured
R1033	T1710	T1711	nummod	27,°C
R1034	T1711	T1709	pobj	°C,at
R1035	T1712	T1707	prep	in,cultured
R1036	T1713	T1714	nmod	Sf,SFM
R1037	T1714	T1712	pobj	SFM,in
R1038	T1715	T1713	punct	-,Sf
R1039	T1716	T1713	nummod	900,Sf
R1040	T1717	T1714	nummod	II,SFM
R1041	T1718	T1719	punct	(,Inc.
R1042	T1719	T1714	parataxis	Inc.,SFM
R1043	T1720	T1719	nmod	Life,Inc.
R1044	T1721	T1719	nmod	Technologies,Inc.
R1045	T1722	T1719	punct	", ",Inc.
R1046	T1723	T1719	punct	),Inc.
R1047	T1724	T1714	cc	and,SFM
R1048	T1725	T1726	nmod	EX,CELL
R1049	T1726	T1728	nmod	CELL,medium
R1050	T1727	T1726	punct	-,CELL
R1051	T1728	T1714	conj	medium,SFM
R1052	T1729	T1726	nummod	405,CELL
R1053	T1730	T1731	punct	(,Biosciences
R1054	T1731	T1728	parataxis	Biosciences,medium
R1055	T1732	T1731	compound	JRH,Biosciences
R1056	T1733	T1731	punct	),Biosciences
R1057	T1734	T1707	punct	", ",cultured
R1058	T1735	T1707	advmod	respectively,cultured
R1059	T1736	T1707	punct	.,cultured
R1060	T1738	T1739	prep	For,coinfected
R1061	T1740	T1738	pobj	expression,For
R1062	T1741	T1740	prep	of,expression
R1063	T1742	T1743	det	the,proteins
R1065	T1744	T1743	nmod	Mcm4,proteins
R1066	T1745	T1744	punct	/,Mcm4
R1067	T1746	T1744	nummod	6,Mcm4
R1068	T1747	T1744	punct	/,Mcm4
R1069	T1748	T1744	nummod	7,Mcm4
R1070	T1749	T1739	punct	", ",coinfected
R1071	T1750	T1751	amod	High,five
R1072	T1751	T1752	nummod	five,cells
R1073	T1752	T1739	nsubjpass	cells,coinfected
R1074	T1753	T1739	auxpass	were,coinfected
R1075	T1754	T1739	prep	with,coinfected
R1076	T1755	T1756	amod	recombinant,baculoviruses
R1077	T1756	T1754	pobj	baculoviruses,with
R1078	T1757	T1756	acl	expressing,baculoviruses
R1079	T1758	T1759	det	the,proteins
R1081	T1760	T1761	compound	His6,Mcm6
R1082	T1761	T1759	compound	Mcm6,proteins
R1083	T1762	T1761	punct	-,Mcm6
R1084	T1763	T1761	compound	Mcm4,Mcm6
R1085	T1764	T1761	punct	/,Mcm6
R1086	T1765	T1756	cc	and,baculoviruses
R1087	T1766	T1756	conj	those,baculoviruses
R1088	T1767	T1766	acl	expressing,those
R1089	T1768	T1769	det	the,FLAG
R1090	T1769	T1767	dobj	FLAG,expressing
R1091	T1770	T1769	compound	Mcm7,FLAG
R1092	T1771	T1769	punct	-,FLAG
R1093	T1772	T1739	punct	", ",coinfected
R1094	T1773	T1739	cc	and,coinfected
R1095	T1774	T1775	auxpass	were,collected
R1096	T1775	T1739	conj	collected,coinfected
R1097	T1776	T1775	prep	at,collected
R1098	T1777	T1778	nummod	48,h
R1099	T1778	T1776	pobj	h,at
R1100	T1779	T1778	advmod	post-infection,h
R1101	T1780	T1739	punct	.,coinfected
R1102	T1782	T1783	det	The,complexes
R1103	T1783	T1790	nsubjpass	complexes,purified
R1104	T1784	T1783	amod	recombinant,complexes
R1105	T1785	T1783	nmod	Mcm4,complexes
R1106	T1786	T1785	punct	/,Mcm4
R1107	T1787	T1785	nummod	6,Mcm4
R1108	T1788	T1785	punct	/,Mcm4
R1109	T1789	T1785	nummod	7,Mcm4
R1110	T1791	T1790	auxpass	were,purified
R1111	T1792	T1793	mark	as,described
R1112	T1793	T1790	advcl	described,purified
R1113	T1794	T1793	advmod	previously,described
R1114	T1795	T1796	punct	(,12
R1115	T1796	T1793	parataxis	12,described
R1116	T1797	T1796	punct	),12
R1117	T1798	T1790	punct	.,purified
R1124	T1980	T1981	compound	DNA,substrates
R1125	T1983	T1984	det	The,sequences
R1126	T1984	T1985	nsubjpass	sequences,listed
R1127	T1986	T1984	prep	for,sequences
R1128	T1987	T1988	predet	all,oligonucleotides
R1129	T1988	T1986	pobj	oligonucleotides,for
R1130	T1989	T1988	det	the,oligonucleotides
R1131	T1990	T1988	acl	used,oligonucleotides
R1132	T1991	T1990	prep	for,used
R1133	T1992	T1991	pobj	constructions,for
R1134	T1993	T1992	prep	of,constructions
R1135	T1994	T1995	compound	DNA,substrates
R1136	T1995	T1993	pobj	substrates,of
R1137	T1996	T1985	auxpass	are,listed
R1138	T1997	T1985	prep	in,listed
R1139	T1998	T1999	nmod	Tables,1
R1140	T1999	T1997	pobj	1,in
R1141	T2000	T1999	cc	and,1
R1142	T2001	T1999	conj	2,1
R1143	T2002	T1985	punct	.,listed
R1144	T2004	T2005	det	The,substrates
R1145	T2005	T2008	nsubjpass	substrates,constructed
R1146	T2006	T2005	amod	partial,substrates
R1147	T2007	T2005	compound	heteroduplex,substrates
R1148	T2009	T2008	auxpass	were,constructed
R1149	T2010	T2008	prep	by,constructed
R1150	T2011	T2010	pcomp	annealing,by
R1151	T2012	T2013	amod	labeled,oligonucleotides
R1152	T2013	T2011	dobj	oligonucleotides,annealing
R1153	T2014	T2011	prep	with,annealing
R1154	T2015	T2016	nmod	M13mp18,DNA
R1155	T2016	T2014	pobj	DNA,with
R1156	T2017	T2018	amod	single,stranded
R1157	T2018	T2016	amod	stranded,DNA
R1158	T2019	T2018	punct	-,stranded
R1159	T2020	T2016	amod	circular,DNA
R1160	T2021	T2016	cc	or,DNA
R1161	T2022	T2023	poss	its,derivative
R1162	T2023	T2016	conj	derivative,DNA
R1163	T2024	T2008	punct	.,constructed
R1164	T2026	T2027	det	The,substrates
R1165	T2027	T2029	nsubjpass	substrates,purified
R1166	T2028	T2027	amod	labeled,substrates
R1167	T2030	T2029	auxpass	were,purified
R1168	T2031	T2029	agent	by,purified
R1169	T2032	T2033	compound	Sepharose,CL4B
R1170	T2033	T2034	compound	CL4B,chromatography
R1171	T2034	T2031	pobj	chromatography,by
R1172	T2035	T2034	compound	column,chromatography
R1173	T2036	T2037	punct	(,Biotech
R1174	T2037	T2029	parataxis	Biotech,purified
R1175	T2038	T2037	compound	Amersham,Biotech
R1176	T2039	T2037	compound	Pharmacia,Biotech
R1177	T2040	T2037	punct	),Biotech
R1178	T2041	T2029	punct	.,purified
R1179	T2043	T2044	det	The,substrates
R1180	T2044	T2047	nsubjpass	substrates,assembled
R1181	T2045	T2046	compound	DNA,bubble
R1182	T2046	T2044	compound	bubble,substrates
R1183	T2048	T2047	auxpass	were,assembled
R1184	T2049	T2047	prep	from,assembled
R1185	T2050	T2051	nummod	two,oligonucleotides
R1186	T2051	T2049	pobj	oligonucleotides,from
R1187	T2052	T2053	advmod	partially,complementary
R1188	T2053	T2051	amod	complementary,oligonucleotides
R1189	T2054	T2051	prep	with,oligonucleotides
R1190	T2055	T2056	amod	top,sequences
R1191	T2056	T2054	pobj	sequences,with
R1192	T2057	T2055	cc	and,top
R1193	T2058	T2055	conj	bottom,top
R1194	T2059	T2056	compound	strand,sequences
R1195	T2060	T2061	punct	(,Table
R1196	T2061	T2051	parataxis	Table,oligonucleotides
R1197	T2062	T2061	nummod	1,Table
R1198	T2063	T2061	punct	),Table
R1199	T2064	T2047	punct	.,assembled
R1200	T2066	T2067	npadvmod	T,tailed
R1201	T2067	T2069	amod	tailed,substrates
R1202	T2068	T2067	punct	-,tailed
R1203	T2069	T2073	nsubjpass	substrates,prepared
R1204	T2070	T2071	compound	Y,fork
R1205	T2071	T2069	compound	fork,substrates
R1206	T2072	T2071	punct	-,fork
R1207	T2074	T2073	auxpass	were,prepared
R1208	T2075	T2073	prep	by,prepared
R1209	T2076	T2075	pcomp	annealing,by
R1210	T2077	T2078	nummod	two,oligonucleotides
R1211	T2078	T2076	dobj	oligonucleotides,annealing
R1212	T2079	T2080	punct	(,series
R1213	T2080	T2078	parataxis	series,oligonucleotides
R1214	T2081	T2080	nmod	dT30,series
R1215	T2082	T2081	cc	and,dT30
R1216	T2083	T2081	conj	dT60,dT30
R1217	T2084	T2080	punct	),series
R1218	T2085	T2073	punct	.,prepared
R1219	T2087	T2088	aux	To,construct
R1220	T2088	T2089	advcl	construct,annealed
R1221	T2090	T2091	det	the,fork
R1222	T2091	T2088	dobj	fork,construct
R1223	T2092	T2091	amod	arrested,fork
R1224	T2093	T2091	cc	and,fork
R1225	T2094	T2095	nummod	3,substrates
R1226	T2095	T2091	conj	substrates,fork
R1227	T2096	T2094	punct	′,3
R1228	T2097	T2094	punct	-,3
R1229	T2098	T2094	cc	or,3
R1230	T2099	T2094	conj	5,3
R1231	T2100	T2099	punct	′,5
R1232	T2101	T2095	punct	-,substrates
R1233	T2102	T2095	compound	extension,substrates
R1234	T2103	T2089	punct	", ",annealed
R1235	T2104	T2105	amod	various,combinations
R1236	T2105	T2089	nsubjpass	combinations,annealed
R1237	T2106	T2105	prep	of,combinations
R1238	T2107	T2106	pobj	oligonucleotides,of
R1239	T2108	T2107	acl	shown,oligonucleotides
R1240	T2109	T2108	prep	in,shown
R1241	T2110	T2109	pobj	Table,in
R1242	T2111	T2110	nummod	1,Table
R1243	T2112	T2089	auxpass	were,annealed
R1244	T2113	T2089	punct	.,annealed
R1245	T2115	T2116	nmod	Bub,B2
R1246	T2116	T2121	nsubjpass	B2,constructed
R1247	T2117	T2116	punct	-,B2
R1248	T2118	T2116	nummod	82,B2
R1249	T2119	T2116	punct	/,B2
R1250	T2120	T2116	compound	lamin,B2
R1251	T2122	T2116	acl	containing,B2
R1252	T2123	T2124	det	the,strand
R1253	T2124	T2122	dobj	strand,containing
R1254	T2125	T2126	npadvmod	T,rich
R1255	T2126	T2124	amod	rich,strand
R1256	T2127	T2126	punct	-,rich
R1257	T2128	T2124	prep	of,strand
R1258	T2129	T2130	det	the,region
R1259	T2130	T2128	pobj	region,of
R1260	T2131	T2132	compound	lamin,B2
R1261	T2132	T2130	compound	B2,region
R1262	T2133	T2130	compound	origin,region
R1263	T2134	T2121	auxpass	was,constructed
R1264	T2135	T2136	mark	as,described
R1265	T2136	T2121	advcl	described,constructed
R1266	T2137	T2136	advmod	previously,described
R1267	T2138	T2139	punct	(,13
R1268	T2139	T2136	parataxis	13,described
R1269	T2140	T2139	punct	),13
R1270	T2141	T2121	punct	.,constructed
R1271	T2143	T2144	det	The,substrate
R1272	T2144	T2146	nsubj	substrate,contains
R1273	T2145	T2144	compound	bubble,substrate
R1274	T2147	T2144	punct	", ",substrate
R1275	T2148	T2149	compound	c,myc
R1276	T2149	T2151	compound	myc,DUE
R1277	T2150	T2149	punct	-,myc
R1278	T2151	T2144	appos	DUE,substrate
R1279	T2152	T2151	punct	/,DUE
R1280	T2153	T2151	cc	or,DUE
R1281	T2154	T2155	compound	c,myc
R1282	T2155	T2157	compound	myc,C
R1283	T2156	T2155	punct	-,myc
R1284	T2157	T2151	conj	C,DUE
R1285	T2158	T2157	punct	/,C
R1286	T2159	T2157	compound	DUE,C
R1287	T2160	T2157	punct	-,C
R1288	T2161	T2146	punct	", ",contains
R1289	T2162	T2146	dobj	sequences,contains
R1290	T2163	T2164	punct	(,K01908
R1291	T2164	T2162	parataxis	K01908,sequences
R1292	T2165	T2166	compound	GenBank,number
R1293	T2166	T2164	compound	number,K01908
R1294	T2167	T2166	compound	accession,number
R1295	T2168	T2164	punct	),K01908
R1296	T2169	T2162	prep	from,sequences
R1297	T2170	T2171	nmod	nucleotides,853
R1298	T2171	T2169	pobj	853,from
R1299	T2172	T2171	nummod	722,853
R1300	T2173	T2171	nmod	to,853
R1301	T2174	T2162	cc	or,sequences
R1302	T2175	T2162	conj	those,sequences
R1303	T2176	T2175	prep	from,those
R1304	T2177	T2176	pobj	828,from
R1305	T2178	T2176	prep	to,from
R1306	T2179	T2178	pobj	747,to
R1307	T2180	T2181	punct	(,strand
R1308	T2181	T2175	parataxis	strand,those
R1309	T2182	T2181	amod	complementary,strand
R1310	T2183	T2181	punct	),strand
R1311	T2184	T2146	punct	", ",contains
R1312	T2185	T2146	advmod	respectively,contains
R1313	T2186	T2146	punct	", ",contains
R1314	T2187	T2146	prep	in,contains
R1315	T2188	T2189	det	the,region
R1316	T2189	T2187	pobj	region,in
R1317	T2190	T2189	amod	central,region
R1318	T2191	T2189	amod	melted,region
R1319	T2192	T2146	punct	.,contains
R1320	T2194	T2195	prep	For,annealed
R1321	T2196	T2197	det	each,substrate
R1322	T2197	T2194	pobj	substrate,For
R1323	T2198	T2195	punct	", ",annealed
R1324	T2199	T2200	amod	labeled,oligonucleotide
R1325	T2200	T2195	nsubjpass	oligonucleotide,annealed
R1326	T2201	T2202	punct	(,pmol
R1327	T2202	T2200	parataxis	pmol,oligonucleotide
R1328	T2203	T2202	nummod	3,pmol
R1329	T2204	T2202	punct	),pmol
R1330	T2205	T2195	auxpass	was,annealed
R1331	T2206	T2195	prep	with,annealed
R1332	T2207	T2208	det	the,oligonucleotide
R1333	T2208	T2206	pobj	oligonucleotide,with
R1334	T2209	T2208	amod	unlabeled,oligonucleotide
R1335	T2210	T2211	punct	(,pmol
R1336	T2211	T2208	parataxis	pmol,oligonucleotide
R1337	T2212	T2211	nummod	6,pmol
R1338	T2213	T2211	punct	),pmol
R1339	T2214	T2195	prep	in,annealed
R1340	T2215	T2216	det	a,mixture
R1341	T2216	T2214	pobj	mixture,in
R1342	T2217	T2216	compound	reaction,mixture
R1343	T2218	T2219	punct	(,μl
R1344	T2219	T2216	parataxis	μl,mixture
R1345	T2220	T2219	nummod	50,μl
R1346	T2221	T2219	punct	),μl
R1347	T2222	T2216	acl	containing,mixture
R1348	T2223	T2224	nummod	20,mM
R1349	T2224	T2225	compound	mM,HCl
R1350	T2225	T2222	dobj	HCl,containing
R1351	T2226	T2225	compound	Tris,HCl
R1352	T2227	T2225	punct	–,HCl
R1353	T2228	T2229	punct	(,pH
R1354	T2229	T2225	parataxis	pH,HCl
R1355	T2230	T2229	nummod	7.5,pH
R1356	T2231	T2229	punct	),pH
R1357	T2232	T2225	punct	", ",HCl
R1358	T2233	T2234	nummod	10,mM
R1359	T2234	T2235	compound	mM,MgCl2
R1360	T2235	T2225	conj	MgCl2,HCl
R1361	T2236	T2235	cc	and,MgCl2
R1362	T2237	T2238	nummod	25,mM
R1363	T2238	T2239	compound	mM,NaCl
R1364	T2239	T2235	conj	NaCl,MgCl2
R1365	T2240	T2216	punct	", ",mixture
R1366	T2241	T2242	dep	which,heated
R1367	T2242	T2216	relcl	heated,mixture
R1368	T2243	T2242	auxpass	were,heated
R1369	T2244	T2242	prep	to,heated
R1370	T2245	T2246	nummod	95,°C
R1371	T2246	T2244	pobj	°C,to
R1372	T2247	T2242	punct	", ",heated
R1373	T2248	T2242	conj	kept,heated
R1374	T2249	T2248	prep	at,kept
R1375	T2250	T2251	nummod	67,°C
R1376	T2251	T2249	pobj	°C,at
R1377	T2252	T2248	prep	for,kept
R1378	T2253	T2254	nummod	1,h
R1379	T2254	T2252	pobj	h,for
R1380	T2255	T2248	punct	", ",kept
R1381	T2256	T2248	cc	and,kept
R1382	T2257	T2258	advmod	then,allowed
R1383	T2258	T2248	conj	allowed,kept
R1384	T2259	T2260	aux	to,cool
R1385	T2260	T2258	xcomp	cool,allowed
R1386	T2261	T2260	advmod	slowly,cool
R1387	T2262	T2260	prt	down,cool
R1388	T2263	T2260	prep	to,cool
R1389	T2264	T2265	nummod	37,°C
R1390	T2265	T2263	pobj	°C,to
R1391	T2266	T2195	punct	.,annealed
R1392	T2268	T2269	det	The,substrates
R1393	T2269	T2271	nsubjpass	substrates,purified
R1394	T2270	T2269	amod	assembled,substrates
R1395	T2272	T2271	auxpass	were,purified
R1396	T2273	T2271	prep	from,purified
R1397	T2274	T2275	compound	polyacrylamide,gel
R1398	T2275	T2273	pobj	gel,from
R1399	T2276	T2271	prep	by,purified
R1400	T2277	T2276	pobj	elution,by
R1401	T2278	T2277	prep	into,elution
R1402	T2279	T2280	compound	TE,buffer
R1403	T2280	T2278	pobj	buffer,into
R1404	T2281	T2282	punct	(,24
R1405	T2282	T2271	parataxis	24,purified
R1406	T2283	T2282	punct	),24
R1407	T2284	T2271	punct	.,purified
R1408	T2286	T2287	det	The,substrates
R1409	T2287	T2290	nsubjpass	substrates,prepared
R1410	T2288	T2287	amod	partial,substrates
R1411	T2289	T2287	compound	heteroduplex,substrates
R1412	T2291	T2287	prep	on,substrates
R1413	T2292	T2293	det	a,DNA
R1414	T2293	T2291	pobj	DNA,on
R1415	T2294	T2295	amod	single,stranded
R1416	T2295	T2293	amod	stranded,DNA
R1417	T2296	T2295	punct	-,stranded
R1418	T2297	T2293	amod	circular,DNA
R1419	T2298	T2293	prep	with,DNA
R1420	T2299	T2300	amod	varied,lengths
R1421	T2300	T2298	pobj	lengths,with
R1422	T2301	T2300	prep	of,lengths
R1423	T2302	T2303	compound	duplex,regions
R1424	T2303	T2301	pobj	regions,of
R1425	T2304	T2290	auxpass	were,prepared
R1426	T2305	T2290	prep	by,prepared
R1427	T2306	T2305	pcomp	extending,by
R1428	T2307	T2308	compound	DNA,chains
R1429	T2308	T2306	dobj	chains,extending
R1430	T2309	T2306	prep	from,extending
R1431	T2310	T2311	det	the,end
R1432	T2311	T2309	pobj	end,from
R1433	T2312	T2311	nummod	3,end
R1434	T2313	T2312	punct	′,3
R1435	T2314	T2311	prep	of,end
R1436	T2315	T2316	det	the,37mer
R1437	T2316	T2314	pobj	37mer,of
R1438	T2317	T2316	compound	dT40,37mer
R1439	T2318	T2316	punct	-,37mer
R1440	T2319	T2316	punct	(,37mer
R1441	T2320	T2321	compound	37mer,region
R1442	T2321	T2316	appos	region,37mer
R1443	T2322	T2321	acl	hybridizing,region
R1444	T2323	T2322	prep	at,hybridizing
R1445	T2324	T2325	nmod	positions,6289
R1446	T2325	T2323	pobj	6289,at
R1447	T2326	T2327	punct	–,6326
R1448	T2327	T2325	prep	6326,6289
R1449	T2328	T2325	prep	of,6289
R1450	T2329	T2330	compound	M13mp18,vector
R1451	T2330	T2328	pobj	vector,of
R1452	T2331	T2316	punct	),37mer
R1453	T2332	T2316	acl	annealed,37mer
R1454	T2333	T2332	prep	to,annealed
R1455	T2334	T2335	det	the,DNA
R1456	T2335	T2333	pobj	DNA,to
R1457	T2336	T2337	amod	single,stranded
R1458	T2337	T2335	amod	stranded,DNA
R1459	T2338	T2337	punct	-,stranded
R1460	T2339	T2335	amod	circular,DNA
R1461	T2340	T2290	punct	.,prepared
R1462	T2342	T2343	compound	DNA,chains
R1463	T2343	T2344	nsubjpass	chains,elongated
R1464	T2345	T2344	auxpass	were,elongated
R1465	T2346	T2344	prep	with,elongated
R1466	T2347	T2346	pobj	Sequenase,with
R1467	T2348	T2344	prep	in,elongated
R1468	T2349	T2350	det	the,presence
R1469	T2350	T2348	pobj	presence,in
R1470	T2351	T2350	prep	of,presence
R1471	T2352	T2353	punct	[,dGTP
R1472	T2353	T2351	pobj	dGTP,of
R1473	T2354	T2355	nmod	α,32P
R1474	T2355	T2353	nmod	32P,dGTP
R1475	T2356	T2355	punct	-,32P
R1476	T2357	T2353	punct	],dGTP
R1477	T2358	T2353	punct	", ",dGTP
R1478	T2359	T2353	acl	followed,dGTP
R1479	T2360	T2359	agent	by,followed
R1480	T2361	T2360	pobj	extension,by
R1481	T2362	T2359	prep	after,followed
R1482	T2363	T2362	pobj	addition,after
R1483	T2364	T2363	prep	of,addition
R1484	T2365	T2364	pobj	ddGTP,of
R1485	T2366	T2365	cc	and,ddGTP
R1486	T2367	T2368	det	all,dNTPs
R1487	T2368	T2365	conj	dNTPs,ddGTP
R1488	T2369	T2368	nummod	four,dNTPs
R1489	T2370	T2344	punct	", ",elongated
R1490	T2371	T2344	advcl	resulting,elongated
R1491	T2372	T2371	prep	in,resulting
R1492	T2373	T2372	pobj	substrates,in
R1493	T2374	T2373	acl	containing,substrates
R1494	T2375	T2376	amod	labeled,regions
R1495	T2376	T2374	dobj	regions,containing
R1496	T2377	T2376	compound	duplex,regions
R1497	T2378	T2376	prep	of,regions
R1498	T2379	T2380	amod	varied,lengths
R1499	T2380	T2378	pobj	lengths,of
R1500	T2381	T2382	punct	(,13
R1501	T2382	T2344	parataxis	13,elongated
R1502	T2383	T2382	punct	),13
R1503	T2384	T2344	punct	.,elongated
R1504	T2386	T2387	det	The,substrate
R1505	T2387	T2388	nsubjpass	substrate,incubated
R1506	T2389	T2390	punct	(,fmol
R1507	T2390	T2387	parataxis	fmol,substrate
R1508	T2391	T2390	nummod	5,fmol
R1509	T2392	T2390	punct	),fmol
R1510	T2393	T2388	auxpass	was,incubated
R1511	T2394	T2388	advmod	first,incubated
R1512	T2395	T2388	prep	with,incubated
R1513	T2396	T2397	amod	indicated,amount
R1514	T2397	T2395	pobj	amount,with
R1515	T2398	T2397	prep	of,amount
R1516	T2399	T2400	det	the,complex
R1517	T2400	T2398	pobj	complex,of
R1518	T2401	T2400	nmod	Mcm4,complex
R1519	T2402	T2401	punct	/,Mcm4
R1520	T2403	T2401	nummod	6,Mcm4
R1521	T2404	T2401	punct	/,Mcm4
R1522	T2405	T2401	nummod	7,Mcm4
R1523	T2406	T2388	prep	at,incubated
R1524	T2407	T2408	nummod	37,°C
R1525	T2408	T2406	pobj	°C,at
R1526	T2409	T2388	prep	for,incubated
R1527	T2410	T2411	nummod	1,h
R1528	T2411	T2409	pobj	h,for
R1529	T2412	T2388	prep	in,incubated
R1530	T2413	T2414	compound	reaction,mixture
R1531	T2414	T2412	pobj	mixture,in
R1532	T2415	T2416	mark	as,described
R1533	T2416	T2388	advcl	described,incubated
R1534	T2417	T2418	punct	(,11
R1535	T2418	T2416	parataxis	11,described
R1536	T2419	T2418	punct	),11
R1537	T2420	T2388	punct	", ",incubated
R1538	T2421	T2388	cc	and,incubated
R1539	T2422	T2421	advmod	then,and
R1540	T2423	T2424	nsubjpass	reactions,stopped
R1541	T2424	T2388	conj	stopped,incubated
R1542	T2425	T2424	auxpass	were,stopped
R1543	T2426	T2424	agent	by,stopped
R1544	T2427	T2426	pobj	addition,by
R1545	T2428	T2427	prep	of,addition
R1546	T2429	T2428	pobj	EDTA,of
R1547	T2430	T2431	punct	(,mM
R1548	T2431	T2429	parataxis	mM,EDTA
R1549	T2432	T2431	nummod	20,mM
R1550	T2433	T2431	punct	),mM
R1551	T2434	T2429	punct	", ",EDTA
R1552	T2435	T2429	conj	SDS,EDTA
R1553	T2436	T2437	punct	(,%
R1554	T2437	T2435	parataxis	%,SDS
R1555	T2438	T2437	nummod	0.1,%
R1556	T2439	T2437	punct	),%
R1557	T2440	T2435	cc	and,SDS
R1558	T2441	T2442	nummod	2,μg
R1559	T2442	T2443	compound	μg,K
R1560	T2443	T2435	conj	K,SDS
R1561	T2444	T2443	compound	proteinase,K
R1562	T2445	T2427	punct	", ",addition
R1563	T2446	T2427	acl	followed,addition
R1564	T2447	T2446	agent	by,followed
R1565	T2448	T2447	pobj	incubation,by
R1566	T2449	T2448	prep	for,incubation
R1567	T2450	T2451	nummod	20,min
R1568	T2451	T2449	pobj	min,for
R1569	T2452	T2424	punct	.,stopped
R1570	T2454	T2455	nsubjpass	Aliquots,electrophoresed
R1571	T2456	T2455	auxpass	were,electrophoresed
R1572	T2457	T2455	prep	on,electrophoresed
R1573	T2458	T2459	det	a,gel
R1574	T2459	T2457	pobj	gel,on
R1575	T2460	T2461	nummod	6.5,%
R1576	T2461	T2459	compound	%,gel
R1577	T2462	T2459	compound	polyacrylamide,gel
R1578	T2463	T2455	prep	in,electrophoresed
R1579	T2464	T2465	nummod	1,TBE
R1580	T2465	T2463	pobj	TBE,in
R1581	T2466	T2464	punct	×,1
R1582	T2467	T2455	prep	at,electrophoresed
R1583	T2468	T2469	nummod	300,V
R1584	T2469	T2467	pobj	V,at
R1585	T2470	T2455	prep	for,electrophoresed
R1586	T2471	T2472	nummod	2.5,h
R1587	T2472	T2470	pobj	h,for
R1588	T2473	T2455	punct	.,electrophoresed
R1589	T2475	T2476	det	The,DNAs
R1590	T2476	T2481	nsubj	DNAs,were
R1591	T2477	T2478	amod	single,stranded
R1592	T2478	T2476	amod	stranded,DNAs
R1593	T2479	T2478	punct	-,stranded
R1594	T2480	T2476	amod	circular,DNAs
R1595	T2482	T2476	acl	used,DNAs
R1596	T2483	T2481	attr	M13mp18,were
R1597	T2484	T2483	cc	and,M13mp18
R1598	T2485	T2483	conj	M13mp19,M13mp18
R1599	T2486	T2483	punct	+,M13mp18
R1600	T2487	T2483	appos	G,M13mp18
R1601	T2488	T2487	punct	-,G
R1602	T2489	T2487	amod	rich,G
R1603	T2490	T2481	punct	.,were
R1604	T2492	T2493	det	The,latter
R1605	T2493	T2494	nsubjpass	latter,constructed
R1606	T2495	T2494	auxpass	was,constructed
R1607	T2496	T2494	prep	by,constructed
R1608	T2497	T2496	pcomp	inserting,by
R1609	T2498	T2499	det	a,sequence
R1610	T2499	T2497	dobj	sequence,inserting
R1611	T2500	T2499	nmod	G,sequence
R1612	T2501	T2500	punct	-,G
R1613	T2502	T2500	amod	rich,G
R1614	T2503	T2499	compound	repeat,sequence
R1615	T2504	T2499	punct	(,sequence
R1616	T2505	T2499	appos	GGGGCGTGGGC,sequence
R1617	T2506	T2505	punct	),GGGGCGTGGGC
R1618	T2507	T2505	nummod	6,GGGGCGTGGGC
R1619	T2508	T2499	punct	", ",sequence
R1620	T2509	T2499	acl	prepared,sequence
R1621	T2510	T2509	prep	by,prepared
R1622	T2511	T2510	pcomp	annealing,by
R1623	T2512	T2511	prep	of,annealing
R1624	T2513	T2514	nummod	two,oligonucleotides
R1625	T2514	T2512	pobj	oligonucleotides,of
R1626	T2515	T2514	punct	(,oligonucleotides
R1627	T2516	T2514	appos	5,oligonucleotides
R1628	T2517	T2516	punct	′,5
R1629	T2518	T2516	punct	-,5
R1630	T2519	T2520	nmod	GATCC,GGGGCGTGGGC
R1631	T2520	T2516	appos	GGGGCGTGGGC,5
R1632	T2521	T2520	punct	-,GGGGCGTGGGC
R1633	T2522	T2520	punct	[,GGGGCGTGGGC
R1634	T2523	T2520	punct	],GGGGCGTGGGC
R1635	T2524	T2520	nummod	6,GGGGCGTGGGC
R1636	T2525	T2516	punct	-,5
R1637	T2526	T2516	nummod	3,5
R1638	T2527	T2516	punct	′,5
R1639	T2528	T2516	punct	/,5
R1640	T2529	T2516	appos	5,5
R1641	T2530	T2529	punct	′,5
R1642	T2531	T2529	punct	-,5
R1643	T2532	T2533	nmod	GATCC,GCCCACGCCCC
R1644	T2533	T2529	appos	GCCCACGCCCC,5
R1645	T2534	T2533	punct	-,GCCCACGCCCC
R1646	T2535	T2533	punct	[,GCCCACGCCCC
R1647	T2536	T2533	punct	],GCCCACGCCCC
R1648	T2537	T2533	nummod	6,GCCCACGCCCC
R1649	T2538	T2529	punct	-,5
R1650	T2539	T2529	nummod	3,5
R1651	T2540	T2529	punct	′,5
R1652	T2541	T2514	punct	),oligonucleotides
R1653	T2542	T2497	prep	at,inserting
R1654	T2543	T2544	det	the,site
R1655	T2544	T2542	pobj	site,at
R1656	T2545	T2544	compound	BamHI,site
R1657	T2546	T2544	prep	of,site
R1658	T2547	T2546	pobj	M13mp19,of
R1659	T2548	T2494	cc	and,constructed
R1660	T2549	T2550	det	the,sequences
R1661	T2550	T2551	nsubjpass	sequences,confirmed
R1662	T2551	T2494	conj	confirmed,constructed
R1663	T2552	T2550	prep	of,sequences
R1664	T2553	T2554	det	the,insert
R1665	T2554	T2552	pobj	insert,of
R1666	T2555	T2551	auxpass	were,confirmed
R1667	T2556	T2551	punct	.,confirmed
R1668	T2629	T2630	nmod	DNA,helicase
R1669	T2630	T2631	nmod	helicase,assays
R1670	T2632	T2630	cc	and,helicase
R1671	T2633	T2634	compound	gel,shift
R1672	T2634	T2630	conj	shift,helicase
R1673	T2636	T2637	prep	In,incubated
R1674	T2638	T2639	compound	gel,shift
R1675	T2639	T2640	compound	shift,assays
R1676	T2640	T2636	pobj	assays,In
R1677	T2641	T2637	punct	", ",incubated
R1678	T2642	T2643	nmod	Mcm4,proteins
R1679	T2643	T2637	nsubjpass	proteins,incubated
R1680	T2644	T2642	punct	/,Mcm4
R1681	T2645	T2642	nummod	6,Mcm4
R1682	T2646	T2642	punct	/,Mcm4
R1683	T2647	T2642	nummod	7,Mcm4
R1684	T2648	T2637	auxpass	were,incubated
R1685	T2649	T2637	prep	at,incubated
R1686	T2650	T2651	nummod	30,°C
R1687	T2651	T2649	pobj	°C,at
R1688	T2652	T2637	prep	for,incubated
R1689	T2653	T2654	nummod	30,min
R1690	T2654	T2652	pobj	min,for
R1691	T2655	T2637	prep	in,incubated
R1692	T2656	T2657	compound	reaction,mixtures
R1693	T2657	T2655	pobj	mixtures,in
R1694	T2658	T2659	punct	(,μl
R1695	T2659	T2657	parataxis	μl,mixtures
R1696	T2660	T2659	nummod	15,μl
R1697	T2661	T2659	punct	),μl
R1698	T2662	T2657	acl	containing,mixtures
R1699	T2663	T2664	nummod	25,mM
R1700	T2664	T2665	compound	mM,NaOH
R1701	T2665	T2662	dobj	NaOH,containing
R1702	T2666	T2665	compound	HEPES,NaOH
R1703	T2667	T2665	punct	–,NaOH
R1704	T2668	T2669	punct	(,pH
R1705	T2669	T2665	parataxis	pH,NaOH
R1706	T2670	T2669	nummod	7.5,pH
R1707	T2671	T2669	punct	),pH
R1708	T2672	T2665	punct	", ",NaOH
R1709	T2673	T2674	nummod	50,mM
R1710	T2674	T2675	compound	mM,acetate
R1711	T2675	T2665	conj	acetate,NaOH
R1712	T2676	T2675	compound	sodium,acetate
R1713	T2677	T2675	punct	", ",acetate
R1714	T2678	T2679	nummod	10,mM
R1715	T2679	T2680	nmod	mM,CH3COO
R1716	T2680	T2675	conj	CH3COO,acetate
R1717	T2681	T2680	nmod	Mg,CH3COO
R1718	T2682	T2680	punct	(,CH3COO
R1719	T2683	T2680	punct	),CH3COO
R1720	T2684	T2680	nummod	2,CH3COO
R1721	T2685	T2680	punct	", ",CH3COO
R1722	T2686	T2687	nummod	1,mM
R1723	T2687	T2688	compound	mM,DTT
R1724	T2688	T2680	conj	DTT,CH3COO
R1725	T2689	T2688	punct	", ",DTT
R1726	T2690	T2691	nummod	0.25,μg
R1727	T2691	T2692	nmod	μg,BSA
R1728	T2692	T2688	conj	BSA,DTT
R1729	T2693	T2694	punct	/,ml
R1730	T2694	T2691	prep	ml,μg
R1731	T2695	T2692	punct	", ",BSA
R1732	T2696	T2697	nummod	0.5,mM
R1733	T2697	T2698	nmod	mM,S
R1734	T2698	T2692	conj	S,BSA
R1735	T2699	T2698	nmod	ATP,S
R1736	T2700	T2698	punct	-,S
R1737	T2701	T2698	punct	γ,S
R1738	T2702	T2698	punct	-,S
R1739	T2703	T2698	cc	and,S
R1740	T2704	T2705	amod	labeled,substrates
R1741	T2705	T2698	conj	substrates,S
R1742	T2706	T2705	prep	of,substrates
R1743	T2707	T2708	det	the,amount
R1744	T2708	T2706	pobj	amount,of
R1745	T2709	T2708	acl	indicated,amount
R1746	T2710	T2637	punct	.,incubated
R1747	T2712	T2713	prep	After,applied
R1748	T2714	T2712	pobj	addition,After
R1749	T2715	T2714	prep	of,addition
R1750	T2716	T2717	nummod	2,μl
R1751	T2717	T2715	pobj	μl,of
R1752	T2718	T2717	prep	of,μl
R1753	T2719	T2720	nummod	50,%
R1754	T2720	T2721	compound	%,glycerol
R1755	T2721	T2718	pobj	glycerol,of
R1756	T2722	T2713	punct	", ",applied
R1757	T2723	T2724	det	the,mixtures
R1758	T2724	T2713	nsubjpass	mixtures,applied
R1759	T2725	T2724	compound	reaction,mixtures
R1760	T2726	T2713	auxpass	were,applied
R1761	T2727	T2713	advmod	directly,applied
R1762	T2728	T2713	prep	on,applied
R1763	T2729	T2730	det	a,gel
R1764	T2730	T2728	pobj	gel,on
R1765	T2731	T2730	compound	polyacrylamide,gel
R1766	T2732	T2730	acl	containing,gel
R1767	T2733	T2734	nummod	6,mM
R1768	T2734	T2735	compound	mM,acetate
R1769	T2735	T2732	dobj	acetate,containing
R1770	T2736	T2735	compound	magnesium,acetate
R1771	T2737	T2735	cc	and,acetate
R1772	T2738	T2739	nummod	5,%
R1773	T2739	T2740	compound	%,glycerol
R1774	T2740	T2735	conj	glycerol,acetate
R1775	T2741	T2732	prep	in,containing
R1776	T2742	T2743	nummod	0.5,TBE
R1777	T2743	T2741	pobj	TBE,in
R1778	T2744	T2742	punct	×,0.5
R1779	T2745	T2713	punct	", ",applied
R1780	T2746	T2713	cc	and,applied
R1781	T2747	T2748	nsubjpass	electrophoresis,conducted
R1782	T2748	T2713	conj	conducted,applied
R1783	T2749	T2748	auxpass	was,conducted
R1784	T2750	T2748	prep	at,conducted
R1785	T2751	T2752	compound	room,temperature
R1786	T2752	T2750	pobj	temperature,at
R1787	T2753	T2748	punct	.,conducted
R1788	T2755	T2756	prep	For,incubated
R1789	T2757	T2758	compound	DNA,assays
R1790	T2758	T2755	pobj	assays,For
R1791	T2759	T2758	compound	helicase,assays
R1792	T2760	T2756	punct	", ",incubated
R1793	T2761	T2762	det	the,mixtures
R1794	T2762	T2756	nsubjpass	mixtures,incubated
R1795	T2763	T2762	compound	reaction,mixtures
R1796	T2764	T2762	prep	of,mixtures
R1797	T2765	T2766	det	the,assays
R1798	T2766	T2764	pobj	assays,of
R1799	T2767	T2768	compound	gel,shift
R1800	T2768	T2766	compound	shift,assays
R1801	T2769	T2756	punct	", ",incubated
R1802	T2770	T2771	mark	as,described
R1803	T2771	T2756	advcl	described,incubated
R1804	T2772	T2771	advmod	above,described
R1805	T2773	T2756	punct	", ",incubated
R1806	T2774	T2756	auxpass	were,incubated
R1807	T2775	T2756	prep	at,incubated
R1808	T2776	T2777	nummod	30,°C
R1809	T2777	T2775	pobj	°C,at
R1810	T2778	T2756	prep	for,incubated
R1811	T2779	T2780	nummod	15,min
R1812	T2780	T2778	pobj	min,for
R1813	T2781	T2756	punct	", ",incubated
R1814	T2782	T2756	cc	and,incubated
R1815	T2783	T2782	advmod	then,and
R1816	T2784	T2785	det	the,ATP
R1817	T2785	T2786	nsubjpass	ATP,added
R1818	T2786	T2756	conj	added,incubated
R1819	T2787	T2786	auxpass	was,added
R1820	T2788	T2786	prep	at,added
R1821	T2789	T2790	nummod	10,mM
R1822	T2790	T2788	pobj	mM,at
R1823	T2791	T2786	punct	", ",added
R1824	T2792	T2786	advcl	followed,added
R1825	T2793	T2792	agent	by,followed
R1826	T2794	T2793	pobj	incubation,by
R1827	T2795	T2794	prep	at,incubation
R1828	T2796	T2797	nummod	37,°C
R1829	T2797	T2795	pobj	°C,at
R1830	T2798	T2794	prep	for,incubation
R1831	T2799	T2800	amod	additional,min
R1832	T2800	T2798	pobj	min,for
R1833	T2801	T2800	nummod	30,min
R1834	T2802	T2786	punct	.,added
R1835	T2804	T2805	det	The,reactions
R1836	T2805	T2806	nsubjpass	reactions,terminated
R1837	T2807	T2806	auxpass	were,terminated
R1838	T2808	T2806	agent	by,terminated
R1839	T2809	T2808	pobj	addition,by
R1840	T2810	T2809	prep	of,addition
R1841	T2811	T2810	pobj	EDTA,of
R1842	T2812	T2813	punct	(,mM
R1843	T2813	T2811	parataxis	mM,EDTA
R1844	T2814	T2813	nummod	20,mM
R1845	T2815	T2813	punct	),mM
R1846	T2816	T2811	punct	", ",EDTA
R1847	T2817	T2811	conj	SDS,EDTA
R1848	T2818	T2819	punct	(,%
R1849	T2819	T2817	parataxis	%,SDS
R1850	T2820	T2819	nummod	0.1,%
R1851	T2821	T2819	punct	),%
R1852	T2822	T2817	cc	and,SDS
R1853	T2823	T2824	nummod	2,μg
R1854	T2824	T2825	compound	μg,K
R1855	T2825	T2817	conj	K,SDS
R1856	T2826	T2825	compound	proteinase,K
R1857	T2827	T2806	punct	", ",terminated
R1858	T2828	T2806	cc	and,terminated
R1859	T2829	T2830	auxpass	were,incubated
R1860	T2830	T2806	conj	incubated,terminated
R1861	T2831	T2830	prep	for,incubated
R1862	T2832	T2833	amod	additional,min
R1863	T2833	T2831	pobj	min,for
R1864	T2834	T2833	nummod	15,min
R1865	T2835	T2806	punct	.,terminated
R1866	T2837	T2838	det	The,samples
R1867	T2838	T2839	nsubjpass	samples,separated
R1868	T2840	T2839	auxpass	were,separated
R1869	T2841	T2839	prep	by,separated
R1870	T2842	T2841	pobj	electrophoresis,by
R1871	T2843	T2839	prep	on,separated
R1872	T2844	T2845	amod	nondenaturing,gel
R1873	T2845	T2843	pobj	gel,on
R1874	T2846	T2845	compound	polyacrylamide,gel
R1875	T2847	T2845	prep	in,gel
R1876	T2848	T2849	nummod	1,TBE
R1877	T2849	T2847	pobj	TBE,in
R1878	T2850	T2848	punct	×,1
R1879	T2851	T2839	punct	.,separated
R1880	T2967	T2968	compound	Nuclease,protection
R1881	T2968	T2969	compound	protection,assays
R1882	T2971	T2972	det	The,mixtures
R1883	T2972	T2974	nsubj	mixtures,contained
R1884	T2973	T2972	compound	reaction,mixtures
R1885	T2975	T2976	punct	(,μl
R1886	T2976	T2972	parataxis	μl,mixtures
R1887	T2977	T2976	nummod	25,μl
R1888	T2978	T2976	punct	),μl
R1889	T2979	T2980	nummod	25,mM
R1890	T2980	T2981	compound	mM,NaOH
R1891	T2981	T2974	dobj	NaOH,contained
R1892	T2982	T2981	compound	HEPES,NaOH
R1893	T2983	T2981	punct	–,NaOH
R1894	T2984	T2985	punct	(,pH
R1895	T2985	T2981	parataxis	pH,NaOH
R1896	T2986	T2985	nummod	7.5,pH
R1897	T2987	T2985	punct	),pH
R1898	T2988	T2981	punct	", ",NaOH
R1899	T2989	T2990	nummod	50,mM
R1900	T2990	T2991	compound	mM,acetate
R1901	T2991	T2981	conj	acetate,NaOH
R1902	T2992	T2991	compound	sodium,acetate
R1903	T2993	T2991	punct	", ",acetate
R1904	T2994	T2995	nummod	10,mM
R1905	T2995	T2996	nmod	mM,CH3COO
R1906	T2996	T2991	conj	CH3COO,acetate
R1907	T2997	T2996	nmod	Mg,CH3COO
R1908	T2998	T2996	punct	(,CH3COO
R1909	T2999	T2996	punct	),CH3COO
R1910	T3000	T2996	nummod	2,CH3COO
R1911	T3001	T2996	punct	", ",CH3COO
R1912	T3002	T3003	nummod	1,mM
R1913	T3003	T3004	compound	mM,DTT
R1914	T3004	T2996	conj	DTT,CH3COO
R1915	T3005	T3004	punct	", ",DTT
R1916	T3006	T3007	nummod	0.25,mg
R1917	T3007	T3008	nmod	mg,BSA
R1918	T3008	T3004	conj	BSA,DTT
R1919	T3009	T3010	punct	/,ml
R1920	T3010	T3007	prep	ml,mg
R1921	T3011	T3008	punct	", ",BSA
R1922	T3012	T3013	nummod	0.5,mM
R1923	T3013	T3014	nmod	mM,S
R1924	T3014	T3008	conj	S,BSA
R1925	T3015	T3014	nmod	ATP,S
R1926	T3016	T3014	punct	-,S
R1927	T3017	T3014	punct	γ,S
R1928	T3018	T3014	punct	-,S
R1929	T3019	T3014	punct	", ",S
R1930	T3020	T3021	nummod	0.5,mM
R1931	T3021	T3022	compound	mM,CaCl2
R1932	T3022	T3014	conj	CaCl2,S
R1933	T3023	T3022	punct	", ",CaCl2
R1934	T3024	T3025	nummod	10,fmol
R1935	T3025	T3022	conj	fmol,CaCl2
R1936	T3026	T3025	prep	of,fmol
R1937	T3027	T3028	npadvmod	32P,labeled
R1938	T3028	T3030	amod	labeled,substrate
R1939	T3029	T3028	punct	-,labeled
R1940	T3030	T3026	pobj	substrate,of
R1941	T3031	T3030	compound	bubble,substrate
R1942	T3032	T3025	punct	", ",fmol
R1943	T3033	T3025	cc	and,fmol
R1944	T3034	T3035	det	the,amount
R1945	T3035	T3025	conj	amount,fmol
R1946	T3036	T3035	amod	indicated,amount
R1947	T3037	T3035	prep	of,amount
R1948	T3038	T3039	nmod	Mcm4,proteins
R1949	T3039	T3037	pobj	proteins,of
R1950	T3040	T3038	punct	/,Mcm4
R1951	T3041	T3038	nummod	6,Mcm4
R1952	T3042	T3038	punct	/,Mcm4
R1953	T3043	T3038	nummod	7,Mcm4
R1954	T3044	T2974	punct	.,contained
R1955	T3046	T3047	prep	After,added
R1956	T3048	T3046	pobj	incubation,After
R1957	T3049	T3048	prep	at,incubation
R1958	T3050	T3051	nummod	30,°C
R1959	T3051	T3049	pobj	°C,at
R1960	T3052	T3048	prep	for,incubation
R1961	T3053	T3054	nummod	30,min
R1962	T3054	T3052	pobj	min,for
R1963	T3055	T3047	punct	", ",added
R1964	T3056	T3057	det	the,amount
R1965	T3057	T3047	nsubjpass	amount,added
R1966	T3058	T3057	amod	indicated,amount
R1967	T3059	T3057	prep	of,amount
R1968	T3060	T3059	pobj	DNase,of
R1969	T3061	T3060	nummod	I,DNase
R1970	T3062	T3063	punct	(,Biomedical
R1971	T3063	T3060	parataxis	Biomedical,DNase
R1972	T3064	T3063	compound	TAKARA,Biomedical
R1973	T3065	T3063	punct	", ",Biomedical
R1974	T3066	T3063	npadvmod	Japan,Biomedical
R1975	T3067	T3063	punct	),Biomedical
R1976	T3068	T3047	auxpass	was,added
R1977	T3069	T3047	punct	", ",added
R1978	T3070	T3047	cc	and,added
R1979	T3071	T3072	det	the,mixtures
R1980	T3072	T3073	nsubjpass	mixtures,incubated
R1981	T3073	T3047	conj	incubated,added
R1982	T3074	T3073	auxpass	were,incubated
R1983	T3075	T3073	prep	at,incubated
R1984	T3076	T3077	compound	room,temperature
R1985	T3077	T3075	pobj	temperature,at
R1986	T3078	T3073	prep	for,incubated
R1987	T3079	T3080	nummod	1,min
R1988	T3080	T3078	pobj	min,for
R1989	T3081	T3047	punct	.,added
R1990	T3083	T3084	nsubjpass	DNase,inactivated
R1991	T3085	T3083	nummod	I,DNase
R1992	T3086	T3084	auxpass	was,inactivated
R1993	T3087	T3084	agent	by,inactivated
R1994	T3088	T3089	det	the,addition
R1995	T3089	T3087	pobj	addition,by
R1996	T3090	T3089	prep	of,addition
R1997	T3091	T3092	nummod	250,μl
R1998	T3092	T3090	pobj	μl,of
R1999	T3093	T3092	prep	of,μl
R2000	T3094	T3095	compound	stop,solution
R2001	T3095	T3093	pobj	solution,of
R2002	T3096	T3097	punct	(,EDTA
R2003	T3097	T3084	parataxis	EDTA,inactivated
R2004	T3098	T3099	nummod	20,mM
R2005	T3099	T3097	compound	mM,EDTA
R2006	T3100	T3097	punct	", ",EDTA
R2007	T3101	T3102	compound	0.2M,chloride
R2008	T3102	T3097	conj	chloride,EDTA
R2009	T3103	T3102	compound	sodium,chloride
R2010	T3104	T3102	punct	", ",chloride
R2011	T3105	T3106	nummod	1,%
R2012	T3106	T3107	compound	%,SDS
R2013	T3107	T3102	conj	SDS,chloride
R2014	T3108	T3107	cc	and,SDS
R2015	T3109	T3110	nummod	12.5,μg
R2016	T3110	T3111	nmod	μg,RNA
R2017	T3111	T3107	conj	RNA,SDS
R2018	T3112	T3113	punct	/,ml
R2019	T3113	T3110	prep	ml,μg
R2020	T3114	T3111	compound	yeast,RNA
R2021	T3115	T3111	punct	),RNA
R2022	T3116	T3084	punct	.,inactivated
R2023	T3118	T3119	prep	For,carried
R2024	T3120	T3121	compound	nuclease,footprinting
R2025	T3121	T3123	compound	footprinting,assay
R2026	T3122	T3121	compound	P1,footprinting
R2027	T3123	T3118	pobj	assay,For
R2028	T3124	T3119	punct	", ",carried
R2029	T3125	T3126	compound	binding,reactions
R2030	T3126	T3119	nsubjpass	reactions,carried
R2031	T3127	T3119	auxpass	were,carried
R2032	T3128	T3119	prt	out,carried
R2033	T3129	T3119	prep	under,carried
R2034	T3130	T3131	det	the,conditions
R2035	T3131	T3129	pobj	conditions,under
R2036	T3132	T3131	amod	same,conditions
R2037	T3133	T3131	prep	except,conditions
R2038	T3134	T3135	mark	that,omitted
R2039	T3135	T3133	pcomp	omitted,except
R2040	T3136	T3135	nsubjpass	CaCl2,omitted
R2041	T3137	T3135	auxpass	was,omitted
R2042	T3138	T3119	punct	.,carried
R2043	T3140	T3141	compound	P1,nuclease
R2044	T3141	T3142	nsubjpass	nuclease,added
R2045	T3143	T3144	punct	(,Roche
R2046	T3144	T3141	parataxis	Roche,nuclease
R2047	T3145	T3146	nummod	0.3,U
R2048	T3146	T3144	dep	U,Roche
R2049	T3147	T3144	punct	", ",Roche
R2050	T3148	T3144	punct	),Roche
R2051	T3149	T3142	auxpass	was,added
R2052	T3150	T3142	cc	and,added
R2053	T3151	T3152	nsubjpass	incubation,continued
R2054	T3152	T3142	conj	continued,added
R2055	T3153	T3152	auxpass	was,continued
R2056	T3154	T3152	prep	at,continued
R2057	T3155	T3156	nummod	37,°C
R2058	T3156	T3154	pobj	°C,at
R2059	T3157	T3152	prep	for,continued
R2060	T3158	T3159	nummod	1,min
R2061	T3159	T3157	pobj	min,for
R2062	T3160	T3152	punct	.,continued
R2063	T3162	T3163	nsubjpass	Cleavage,stopped
R2064	T3163	T3165	ccomp	stopped,incubated
R2065	T3164	T3163	auxpass	was,stopped
R2066	T3166	T3163	prep	by,stopped
R2067	T3167	T3166	pobj	addition,by
R2068	T3168	T3167	prep	of,addition
R2069	T3169	T3170	nummod	50,μl
R2070	T3170	T3168	pobj	μl,of
R2071	T3171	T3170	prep	of,μl
R2072	T3172	T3173	nummod	25,mM
R2073	T3173	T3174	compound	mM,EDTA
R2074	T3174	T3171	pobj	EDTA,of
R2075	T3175	T3174	cc	and,EDTA
R2076	T3176	T3177	nummod	0.4,M
R2077	T3177	T3178	compound	M,NaOH
R2078	T3178	T3174	conj	NaOH,EDTA
R2079	T3179	T3165	punct	;,incubated
R2080	T3180	T3181	det	the,mixture
R2081	T3181	T3165	nsubjpass	mixture,incubated
R2082	T3182	T3165	auxpass	was,incubated
R2083	T3183	T3165	prep	at,incubated
R2084	T3184	T3185	compound	room,temperature
R2085	T3185	T3183	pobj	temperature,at
R2086	T3186	T3165	prep	for,incubated
R2087	T3187	T3188	nummod	10,min
R2088	T3188	T3186	pobj	min,for
R2089	T3189	T3188	acl	followed,min
R2090	T3190	T3189	agent	by,followed
R2091	T3191	T3192	det	the,addition
R2092	T3192	T3190	pobj	addition,by
R2093	T3193	T3192	prep	of,addition
R2094	T3194	T3195	nummod	12,μl
R2095	T3195	T3193	pobj	μl,of
R2096	T3196	T3195	prep	of,μl
R2097	T3197	T3198	nummod	3,M
R2098	T3198	T3199	compound	M,acetate
R2099	T3199	T3196	pobj	acetate,of
R2100	T3200	T3199	compound	sodium,acetate
R2101	T3201	T3202	punct	(,pH
R2102	T3202	T3199	parataxis	pH,acetate
R2103	T3203	T3202	nummod	4.8,pH
R2104	T3204	T3202	punct	),pH
R2105	T3205	T3165	punct	.,incubated
R2106	T3207	T3208	nsubjpass	Proteins,removed
R2107	T3209	T3208	auxpass	were,removed
R2108	T3210	T3208	prep	by,removed
R2109	T3211	T3212	compound	phenol,chloroform
R2110	T3212	T3214	compound	chloroform,extraction
R2111	T3213	T3212	punct	–,chloroform
R2112	T3214	T3210	pobj	extraction,by
R2113	T3215	T3208	punct	", ",removed
R2114	T3216	T3208	cc	and,removed
R2115	T3217	T3218	nsubjpass	DNAs,collected
R2116	T3218	T3208	conj	collected,removed
R2117	T3219	T3218	auxpass	were,collected
R2118	T3220	T3218	prep	by,collected
R2119	T3221	T3222	compound	ethanol,precipitation
R2120	T3222	T3220	pobj	precipitation,by
R2121	T3223	T3222	acl	followed,precipitation
R2122	T3224	T3223	agent	by,followed
R2123	T3225	T3224	pobj	wash,by
R2124	T3226	T3225	prep	with,wash
R2125	T3227	T3228	nummod	70,%
R2126	T3228	T3229	compound	%,ethanol
R2127	T3229	T3226	pobj	ethanol,with
R2128	T3230	T3218	punct	.,collected
R2129	T3232	T3233	det	The,DNA
R2130	T3233	T3235	nsubjpass	DNA,subjected
R2131	T3234	T3233	amod	digested,DNA
R2132	T3236	T3235	auxpass	was,subjected
R2133	T3237	T3235	prep	to,subjected
R2134	T3238	T3237	pobj	electrophoresis,to
R2135	T3239	T3238	prep	through,electrophoresis
R2136	T3240	T3241	det	a,gel
R2137	T3241	T3239	pobj	gel,through
R2138	T3242	T3243	nummod	10,%
R2139	T3243	T3241	compound	%,gel
R2140	T3244	T3242	cc	or,10
R2141	T3245	T3242	conj	12,10
R2142	T3246	T3241	compound	polyacrylamide,gel
R2143	T3247	T3241	compound	sequencing,gel
R2144	T3248	T3241	acl	containing,gel
R2145	T3249	T3250	nummod	7,M
R2146	T3250	T3251	compound	M,urea
R2147	T3251	T3248	dobj	urea,containing
R2148	T3252	T3238	punct	", ",electrophoresis
R2149	T3253	T3238	acl	followed,electrophoresis
R2150	T3254	T3253	agent	by,followed
R2151	T3255	T3254	pobj	autoradiography,by
R2152	T3256	T3235	punct	.,subjected
R999	T1672	T1670	pobj	complex,of
R1064	T1743	T1741	pobj	proteins,of
R1080	T1759	T1757	dobj	proteins,expressing

craft-ca-core-ex-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id	Subject	Object	Predicate	Lexical cue
T1431	177-187	CHEBI_SO_EXT:nucleotide	denotes	nucleotide
T1432	196-203	GO_PR_EXT:deoxyribonuclease_I	denotes	DNase I
T1433	246-254	GO_EXT:0004518	denotes	Nuclease
T1435	295-303	GO:0042571	denotes	antibody
T1436	305-312	CHEBI:2511	denotes	agarose
T1437	322-329	CHEBI_SO_EXT:peptide_or_peptide_region	denotes	peptide
T1438	347-351	NCBITaxon:10633	denotes	SV40
T1439	354-361	CHEBI_EXT:59132	denotes	antigen
T1440	380-391	NCBITaxon:10442	denotes	baculovirus
T1441	401-407	NCBITaxon:6960	denotes	insect
T1442	408-413	CL_GO_EXT:cell	denotes	cells
T1443	432-436	PR_EXT:000023591	denotes	PriA
T1444	437-445	GO_EXT:0004386	denotes	helicase
T1445	446-453	CHEBI_PR_EXT:protein	denotes	protein
T1446	491-507	CHEBI_SO_EXT:oligonucleotide	denotes	Oligonucleotides
T1447	601-615	CHEBI_EXT:polyacrylamide	denotes	polyacrylamide
T1635	694-704	GO:0010467	denotes	Expression
T1636	725-730	NCBITaxon:10088	denotes	mouse
T1637	731-735	PR_EXT:000010246	denotes	Mcm4
T1638	731-747	GO:0097373	denotes	Mcm4/6/7 complex
T1639	752-763	GO_SO_EXT:sequence_rearrangement_process	denotes	recombinant
T1640	764-777	NCBITaxon:10442	denotes	baculoviruses
T1641	778-788	GO:0010467	denotes	expressing
T1642	789-792	CHEBI_SO_EXT:histidine	denotes	His
T1643	794-798	PR_EXT:000010246	denotes	Mcm4
T1644	799-803	PR_EXT:000010248	denotes	Mcm6
T1645	807-811	PR_EXT:000010249	denotes	Mcm7
T1646	817-825	CHEBI_PR_EXT:protein	denotes	proteins
T1647	876-882	NCBITaxon:6960	denotes	insect
T1648	883-888	CL_GO_EXT:cell	denotes	cells
T1649	961-965	CL_GO_EXT:cell	denotes	CELL
T1650	1014-1024	GO:0010467	denotes	expression
T1651	1032-1036	PR_EXT:000010246	denotes	Mcm4
T1652	1032-1040	GO:0097373	denotes	Mcm4/6/7
T1653	1041-1049	CHEBI_PR_EXT:protein	denotes	proteins
T1654	1061-1066	CL_GO_EXT:cell	denotes	cells
T1655	1088-1099	GO_SO_EXT:sequence_rearrangement_process	denotes	recombinant
T1656	1100-1113	NCBITaxon:10442	denotes	baculoviruses
T1657	1114-1124	GO:0010467	denotes	expressing
T1658	1129-1132	CHEBI_SO_EXT:histidine	denotes	His
T1659	1134-1138	PR_EXT:000010246	denotes	Mcm4
T1660	1139-1143	PR_EXT:000010248	denotes	Mcm6
T1661	1144-1152	CHEBI_PR_EXT:protein	denotes	proteins
T1662	1163-1173	GO:0010467	denotes	expressing
T1663	1178-1182	PR_EXT:000010249	denotes	Mcm7
T1664	1236-1247	GO_SO_EXT:sequence_rearrangement_process	denotes	recombinant
T1665	1248-1252	PR_EXT:000010246	denotes	Mcm4
T1666	1248-1266	GO:0097373	denotes	Mcm4/6/7 complexes
T1897	1312-1315	CHEBI_SO_EXT:DNA	denotes	DNA
T1898	1331-1340	SO_EXT:biological_sequence	denotes	sequences
T1899	1353-1369	CHEBI_SO_EXT:oligonucleotide	denotes	oligonucleotides
T1900	1396-1399	CHEBI_SO_EXT:DNA	denotes	DNA
T1901	1459-1465	SO:0000985	denotes	duplex
T1902	1497-1506	GO:0097617	denotes	annealing
T1903	1507-1514	CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process	denotes	labeled
T1904	1515-1531	CHEBI_SO_EXT:oligonucleotide	denotes	oligonucleotides
T1905	1545-1560	_FRAGMENT	denotes	single-stranded
T1906	1570-1573	CHEBI_SO_EXT:single_stranded_DNA	denotes	DNA
T1907	1561-1569	SO:0000988	denotes	circular
T1908	1597-1604	CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process	denotes	labeled
T1909	1704-1707	CHEBI_SO_EXT:DNA	denotes	DNA
T1910	1774-1790	CHEBI_SO_EXT:oligonucleotide	denotes	oligonucleotides
T1911	1818-1827	SO_EXT:biological_sequence	denotes	sequences
T1912	1850-1854	GO:0005657	denotes	fork
T1913	1883-1892	GO:0097617	denotes	annealing
T1914	1897-1913	CHEBI_SO_EXT:oligonucleotide	denotes	oligonucleotides
T1915	1964-1968	GO:0005657	denotes	fork
T1916	1973-1976	_FRAGMENT	denotes	3′-
T1917	1983-1992	GO:0006272	denotes	extension
T1918	1980-1992	GO:0006273	denotes	5′-extension
T1919	2029-2045	CHEBI_SO_EXT:oligonucleotide	denotes	oligonucleotides
T1920	2068-2076	GO:0097617	denotes	annealed
T1921	2085-2093	PR_EXT:000009861	denotes	lamin B2
T1922	2109-2110	CHEBI_EXT:thymine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	T
T1923	2130-2138	PR_EXT:000009861	denotes	lamin B2
T1924	2139-2145	SO_EXT:0000296	denotes	origin
T1925	2221-2226	PR_EXT:000000084	denotes	c-myc
T1926	2234-2239	PR_EXT:000000084	denotes	c-myc
T1927	2256-2265	SO_EXT:biological_sequence	denotes	sequences
T1928	2305-2316	CHEBI_SO_EXT:nucleotide	denotes	nucleotides
T1929	2406-2412	GO:0032508	denotes	melted
T1930	2441-2448	CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process	denotes	labeled
T1931	2449-2464	CHEBI_SO_EXT:oligonucleotide	denotes	oligonucleotide
T1932	2478-2486	GO:0097617	denotes	annealed
T1933	2498-2505	CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process	denotes	labeled
T1934	2506-2521	CHEBI_SO_EXT:oligonucleotide	denotes	oligonucleotide
T1935	2545-2552	CHEBI:60004	denotes	mixture
T1936	2578-2582	CHEBI:9754	denotes	Tris
T1937	2583-2586	CHEBI:17883	denotes	HCl
T1938	2603-2608	CHEBI:6636	denotes	MgCl2
T1939	2619-2623	CHEBI:26710	denotes	NaCl
T1940	2764-2778	CHEBI_EXT:polyacrylamide	denotes	polyacrylamide
T1941	2802-2808	CHEBI_CHMO_EXT:buffer_solution	denotes	buffer
T1942	2833-2839	SO:0000985	denotes	duplex
T1943	2856-2871	_FRAGMENT	denotes	single-stranded
T1944	2881-2884	CHEBI_SO_EXT:single_stranded_DNA	denotes	DNA
T1945	2872-2880	SO:0000988	denotes	circular
T1946	2908-2914	SO:0000985	denotes	duplex
T1947	2950-2953	CHEBI_SO_EXT:DNA	denotes	DNA
T1948	3009-3020	GO:0097617	denotes	hybridizing
T1949	3055-3061	SO_EXT:0000440	denotes	vector
T1950	3063-3071	GO:0097617	denotes	annealed
T1951	3079-3094	_FRAGMENT	denotes	single-stranded
T1952	3104-3107	CHEBI_SO_EXT:single_stranded_DNA	denotes	DNA
T1953	3095-3103	SO:0000988	denotes	circular
T1954	3109-3112	CHEBI_SO_EXT:DNA	denotes	DNA
T1955	3172-3175	CHEBI:37972	denotes	32P
T1956	3176-3180	CHEBI_EXT:dGTP	denotes	dGTP
T1957	3241-3246	CHEBI_EXT:dNTP	denotes	dNTPs
T1958	3283-3290	CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process	denotes	labeled
T1959	3291-3297	SO:0000985	denotes	duplex
T1960	3402-3406	PR_EXT:000010246	denotes	Mcm4
T1961	3402-3418	GO:0097373	denotes	Mcm4/6/7 complex
T1962	3447-3454	CHEBI:60004	denotes	mixture
T1963	3521-3525	CHEBI_EXT:EDTA	denotes	EDTA
T1964	3535-3538	CHEBI:8984	denotes	SDS
T1965	3555-3565	GO_EXT:0008233	denotes	proteinase
T1966	3644-3658	CHEBI_EXT:polyacrylamide	denotes	polyacrylamide
T1967	3697-3712	_FRAGMENT	denotes	single-stranded
T1968	3722-3726	CHEBI_SO_EXT:single_stranded_DNA	denotes	DNAs
T1969	3713-3721	SO:0000988	denotes	circular
T1970	3757-3758	CHEBI_EXT:guanine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	G
T1971	3795-3804	SO_EXT:sequence_insertion_process	denotes	inserting
T1972	3807-3808	CHEBI_EXT:guanine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	G
T1973	3814-3820	SO_EXT:sequence_repeat_unit_or_region	denotes	repeat
T1974	3821-3829	SO_EXT:biological_sequence	denotes	sequence
T1975	3858-3867	GO:0097617	denotes	annealing
T1976	3875-3891	CHEBI_SO_EXT:oligonucleotide	denotes	oligonucleotides
T1977	3955-3960	PR_EXT:P23940	denotes	BamHI
T1978	3985-3994	SO_EXT:biological_sequence	denotes	sequences
T1979	4002-4008	SO_EXT:0000667	denotes	insert
T2602	4026-4029	CHEBI_SO_EXT:DNA	denotes	DNA
T2603	4026-4038	GO_EXT:0003678	denotes	DNA helicase
T2604	4081-4085	PR_EXT:000010246	denotes	Mcm4
T2605	4081-4089	GO:0097373	denotes	Mcm4/6/7
T2606	4090-4098	CHEBI_PR_EXT:protein	denotes	proteins
T2607	4145-4153	CHEBI:60004	denotes	mixtures
T2608	4179-4184	CHEBI:46756	denotes	HEPES
T2609	4185-4189	CHEBI:32145	denotes	NaOH
T2610	4206-4220	CHEBI:32954	denotes	sodium acetate
T2611	4228-4239	CHEBI:62964	denotes	Mg(CH3COO)2
T2612	4246-4249	CHEBI:18320	denotes	DTT
T2613	4274-4281	CHEBI:27575	denotes	ATP-γ-S
T2614	4286-4293	CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process	denotes	labeled
T2615	4360-4368	CHEBI:17754	denotes	glycerol
T2616	4383-4391	CHEBI:60004	denotes	mixtures
T2617	4419-4433	CHEBI_EXT:polyacrylamide	denotes	polyacrylamide
T2618	4454-4471	CHEBI:62964	denotes	magnesium acetate
T2619	4479-4487	CHEBI:17754	denotes	glycerol
T2620	4560-4563	CHEBI_SO_EXT:DNA	denotes	DNA
T2621	4560-4572	GO_EXT:0003678	denotes	DNA helicase
T2622	4594-4602	CHEBI:60004	denotes	mixtures
T2623	4696-4699	CHEBI_EXT:ATP	denotes	ATP
T2624	4819-4823	CHEBI_EXT:EDTA	denotes	EDTA
T2625	4833-4836	CHEBI:8984	denotes	SDS
T2626	4853-4863	GO_EXT:0008233	denotes	proteinase
T2627	4961-4971	GO_EXT:macromolecule_denaturation	denotes	denaturing
T2628	4972-4986	CHEBI_EXT:polyacrylamide	denotes	polyacrylamide
T2926	5003-5011	GO_EXT:0004518	denotes	Nuclease
T2927	5043-5051	CHEBI:60004	denotes	mixtures
T2928	5076-5081	CHEBI:46756	denotes	HEPES
T2929	5082-5086	CHEBI:32145	denotes	NaOH
T2930	5103-5117	CHEBI:32954	denotes	sodium acetate
T2931	5125-5136	CHEBI:62964	denotes	Mg(CH3COO)2
T2932	5143-5146	CHEBI:18320	denotes	DTT
T2933	5171-5178	CHEBI:27575	denotes	ATP-γ-S
T2934	5187-5192	CHEBI:3312	denotes	CaCl2
T2935	5205-5208	CHEBI:37972	denotes	32P
T2936	5209-5216	CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process	denotes	labeled
T2937	5263-5267	PR_EXT:000010246	denotes	Mcm4
T2938	5263-5271	GO:0097373	denotes	Mcm4/6/7
T2939	5272-5280	CHEBI_PR_EXT:protein	denotes	proteins
T2940	5343-5350	GO_PR_EXT:deoxyribonuclease_I	denotes	DNase I
T2941	5397-5405	CHEBI:60004	denotes	mixtures
T2942	5452-5459	GO_PR_EXT:deoxyribonuclease_I	denotes	DNase I
T2943	5510-5518	CHEBI:75958	denotes	solution
T2944	5526-5530	CHEBI_EXT:EDTA	denotes	EDTA
T2945	5537-5552	CHEBI:26710	denotes	sodium chloride
T2946	5557-5560	CHEBI:8984	denotes	SDS
T2947	5576-5581	NCBITaxon_EXT:yeast	denotes	yeast
T2948	5582-5585	CHEBI_SO_EXT:RNA	denotes	RNA
T2949	5592-5600	GO_EXT:0004518	denotes	nuclease
T2950	5624-5631	CHEMINF_GO_EXT:chemical_binding_or_bond_formation	denotes	binding
T2951	5697-5702	CHEBI:3312	denotes	CaCl2
T2952	5719-5727	GO_EXT:0004518	denotes	nuclease
T2953	5801-5809	MOP:0000780	denotes	Cleavage
T2954	5852-5856	CHEBI_EXT:EDTA	denotes	EDTA
T2955	5867-5871	CHEBI:32145	denotes	NaOH
T2956	5877-5884	CHEBI:60004	denotes	mixture
T2957	5971-5985	CHEBI:32954	denotes	sodium acetate
T2958	5996-6004	CHEBI_PR_EXT:protein	denotes	Proteins
T2959	6021-6027	CHEBI:15882	denotes	phenol
T2960	6028-6038	CHEBI:35255	denotes	chloroform
T2961	6055-6059	CHEBI_SO_EXT:DNA	denotes	DNAs
T2962	6078-6085	CHEBI:16236	denotes	ethanol
T2963	6126-6133	CHEBI:16236	denotes	ethanol
T2964	6148-6151	CHEBI_SO_EXT:DNA	denotes	DNA
T2965	6205-6219	CHEBI_EXT:polyacrylamide	denotes	polyacrylamide
T2966	6250-6254	CHEBI:16199	denotes	urea
T1419	23-31	CHEBI_EXT:33893	denotes	Reagents
T1420	32-39	CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process	denotes	Labeled
T1421	46-53	CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process	denotes	labeled
T1422	54-59	CHEBI_EXT:dNTP	denotes	dNTPs
T1423	60-65	CHEBI_EXT:ribonucleoside_triphosphate	denotes	rNTPs
T1424	140-155	_FRAGMENT	denotes	single-stranded
T1425	165-168	CHEBI_SO_EXT:single_stranded_DNA	denotes	DNA
T1426	156-164	SO:0000988	denotes	circular
T1427	170-172	NCBITaxon:10665	denotes	T4
T1428	173-187	CHEBI:15986	denotes	polynucleotide
T1429	173-194	PR_EXT:000012924	denotes	polynucleotide kinase
T1430	173-194	GO_EXT:0051731	denotes	polynucleotide kinase
T1434	291-293	GO:0042571	denotes	Ab
R871	T1425	T1424	_lexicallyChainedTo	DNA,single-stranded
R1119	T1906	T1905	_lexicallyChainedTo	DNA,single-stranded
R1120	T1917	T1916	_lexicallyChainedTo	extension,3′-
R1121	T1944	T1943	_lexicallyChainedTo	DNA,single-stranded
R1122	T1952	T1951	_lexicallyChainedTo	DNA,single-stranded
R1123	T1968	T1967	_lexicallyChainedTo	DNAs,single-stranded

craft-ca-core-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id	Subject	Object	Predicate	Lexical cue
T1415	354-361	CHEBI:59132	denotes	antigen
T1416	380-391	NCBITaxon:10442	denotes	baculovirus
T1417	401-407	NCBITaxon:6960	denotes	insect
T1418	432-436	PR:000023591	denotes	PriA
T1854	1459-1465	SO:0000985	denotes	duplex
T1855	1497-1506	GO:0097617	denotes	annealing
T1856	1545-1560	SO:0000984	denotes	single-stranded
T1857	1561-1569	SO:0000988	denotes	circular
T1858	1850-1854	GO:0005657	denotes	fork
T1859	1883-1892	GO:0097617	denotes	annealing
T1860	1964-1968	GO:0005657	denotes	fork
T1861	1973-1976	_FRAGMENT	denotes	3′-
T1862	1983-1992	GO:0006272	denotes	extension
T1863	1980-1992	GO:0006273	denotes	5′-extension
T1864	2068-2076	GO:0097617	denotes	annealed
T1865	2085-2093	PR:000009861	denotes	lamin B2
T1866	2130-2138	PR:000009861	denotes	lamin B2
T1867	2139-2145	SO:0000296	denotes	origin
T1868	2221-2226	PR:000000084	denotes	c-myc
T1869	2234-2239	PR:000000084	denotes	c-myc
T1870	2406-2412	GO:0032508	denotes	melted
T1871	2478-2486	GO:0097617	denotes	annealed
T1872	2545-2552	CHEBI:60004	denotes	mixture
T1873	2578-2582	CHEBI:9754	denotes	Tris
T1874	2583-2586	CHEBI:17883	denotes	HCl
T1875	2603-2608	CHEBI:6636	denotes	MgCl2
T1876	2619-2623	CHEBI:26710	denotes	NaCl
T1877	2833-2839	SO:0000985	denotes	duplex
T1878	2856-2871	SO:0000984	denotes	single-stranded
T1879	2872-2880	SO:0000988	denotes	circular
T1880	2908-2914	SO:0000985	denotes	duplex
T1881	3009-3020	GO:0097617	denotes	hybridizing
T1882	3055-3061	SO:0000440	denotes	vector
T1883	3063-3071	GO:0097617	denotes	annealed
T1884	3079-3094	SO:0000984	denotes	single-stranded
T1885	3095-3103	SO:0000988	denotes	circular
T1886	3172-3175	CHEBI:37972	denotes	32P
T1887	3291-3297	SO:0000985	denotes	duplex
T1888	3402-3406	PR:000010246	denotes	Mcm4
T1889	3402-3418	GO:0097373	denotes	Mcm4/6/7 complex
T1890	3447-3454	CHEBI:60004	denotes	mixture
T1891	3535-3538	CHEBI:8984	denotes	SDS
T1892	3697-3712	SO:0000984	denotes	single-stranded
T1893	3713-3721	SO:0000988	denotes	circular
T1894	3858-3867	GO:0097617	denotes	annealing
T1895	3955-3960	PR:P23940	denotes	BamHI
T1896	4002-4008	SO:0000667	denotes	insert
T2587	4081-4085	PR:000010246	denotes	Mcm4
T2588	4081-4089	GO:0097373	denotes	Mcm4/6/7
T2589	4145-4153	CHEBI:60004	denotes	mixtures
T2590	4179-4184	CHEBI:46756	denotes	HEPES
T2591	4185-4189	CHEBI:32145	denotes	NaOH
T2592	4206-4220	CHEBI:32954	denotes	sodium acetate
T2593	4228-4239	CHEBI:62964	denotes	Mg(CH3COO)2
T2594	4246-4249	CHEBI:18320	denotes	DTT
T2595	4274-4281	CHEBI:27575	denotes	ATP-γ-S
T2596	4360-4368	CHEBI:17754	denotes	glycerol
T2597	4383-4391	CHEBI:60004	denotes	mixtures
T2598	4454-4471	CHEBI:62964	denotes	magnesium acetate
T2599	4479-4487	CHEBI:17754	denotes	glycerol
T2600	4594-4602	CHEBI:60004	denotes	mixtures
T2601	4833-4836	CHEBI:8984	denotes	SDS
T2901	5043-5051	CHEBI:60004	denotes	mixtures
T2902	5076-5081	CHEBI:46756	denotes	HEPES
T2903	5082-5086	CHEBI:32145	denotes	NaOH
T2904	5103-5117	CHEBI:32954	denotes	sodium acetate
T2905	5125-5136	CHEBI:62964	denotes	Mg(CH3COO)2
T2906	5143-5146	CHEBI:18320	denotes	DTT
T2907	5171-5178	CHEBI:27575	denotes	ATP-γ-S
T2908	5187-5192	CHEBI:3312	denotes	CaCl2
T2909	5205-5208	CHEBI:37972	denotes	32P
T2910	5263-5267	PR:000010246	denotes	Mcm4
T2911	5263-5271	GO:0097373	denotes	Mcm4/6/7
T2912	5397-5405	CHEBI:60004	denotes	mixtures
T2913	5510-5518	CHEBI:75958	denotes	solution
T2914	5537-5552	CHEBI:26710	denotes	sodium chloride
T2915	5557-5560	CHEBI:8984	denotes	SDS
T2916	5697-5702	CHEBI:3312	denotes	CaCl2
T2917	5801-5809	MOP:0000780	denotes	Cleavage
T2918	5867-5871	CHEBI:32145	denotes	NaOH
T2919	5877-5884	CHEBI:60004	denotes	mixture
T2920	5971-5985	CHEBI:32954	denotes	sodium acetate
T2921	6021-6027	CHEBI:15882	denotes	phenol
T2922	6028-6038	CHEBI:35255	denotes	chloroform
T2923	6078-6085	CHEBI:16236	denotes	ethanol
T2924	6126-6133	CHEBI:16236	denotes	ethanol
T2925	6250-6254	CHEBI:16199	denotes	urea
T1405	23-31	CHEBI:33893	denotes	Reagents
T1406	140-155	SO:0000984	denotes	single-stranded
T1407	156-164	SO:0000988	denotes	circular
T1408	170-172	NCBITaxon:10665	denotes	T4
T1409	173-187	CHEBI:15986	denotes	polynucleotide
T1410	173-194	PR:000012924	denotes	polynucleotide kinase
T1411	291-293	GO:0042571	denotes	Ab
T1412	295-303	GO:0042571	denotes	antibody
T1413	305-312	CHEBI:2511	denotes	agarose
T1414	347-351	NCBITaxon:10633	denotes	SV40
T1614	694-704	GO:0010467	denotes	Expression
T1615	725-730	NCBITaxon:10088	denotes	mouse
T1616	731-735	PR:000010246	denotes	Mcm4
T1617	731-747	GO:0097373	denotes	Mcm4/6/7 complex
T1618	764-777	NCBITaxon:10442	denotes	baculoviruses
T1619	778-788	GO:0010467	denotes	expressing
T1620	794-798	PR:000010246	denotes	Mcm4
T1621	799-803	PR:000010248	denotes	Mcm6
T1622	807-811	PR:000010249	denotes	Mcm7
T1623	876-882	NCBITaxon:6960	denotes	insect
T1624	1014-1024	GO:0010467	denotes	expression
T1625	1032-1036	PR:000010246	denotes	Mcm4
T1626	1032-1040	GO:0097373	denotes	Mcm4/6/7
T1627	1100-1113	NCBITaxon:10442	denotes	baculoviruses
T1628	1114-1124	GO:0010467	denotes	expressing
T1629	1134-1138	PR:000010246	denotes	Mcm4
T1630	1139-1143	PR:000010248	denotes	Mcm6
T1631	1163-1173	GO:0010467	denotes	expressing
T1632	1178-1182	PR:000010249	denotes	Mcm7
T1633	1248-1252	PR:000010246	denotes	Mcm4
T1634	1248-1266	GO:0097373	denotes	Mcm4/6/7 complexes
R1118	T1862	T1861	_lexicallyChainedTo	extension,3′-

2_test

Id	Subject	Object	Predicate	Lexical cue
15917436-8390661-76775501	427-429	8390661	denotes	22
15917436-12151393-76775502	486-488	12151393	denotes	23
15917436-10567526-76775503	853-855	10567526	denotes	11
15917436-12207017-76775504	1306-1308	12207017	denotes	12
15917436-14609960-76775505	2194-2196	14609960	denotes	13
15917436-14609960-76775506	3325-3327	14609960	denotes	13
15917436-10567526-76775507	3469-3471	10567526	denotes	11

PMC:1140370 / 4609-10893 JSONTXT

Annnotations TAB JSON ListView MergeView

craft-sa-dev

craft-ca-core-ex-dev

craft-ca-core-dev

2_test

PMC:1140370 / 4609-10893 JSON TXT