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PMC:1140370 / 13209-15808 JSONTXT

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Id Subject Object Predicate Lexical cue
T4077 0-9 NN denotes Formation
T4078 49-60 VBZ denotes facilitates
T4079 10-12 IN denotes of
T4080 13-19 JJ denotes double
T4081 20-29 JJ denotes hexameric
T4082 39-48 NNS denotes complexes
T4083 30-34 NN denotes Mcm4
T4084 34-35 HYPH denotes /
T4085 35-36 CD denotes 6
T4086 36-37 HYPH denotes /
T4087 37-38 CD denotes 7
T4088 61-69 NN denotes helicase
T4089 70-77 NNS denotes actions
T4090 78-80 IN denotes on
T4091 81-87 NN denotes bubble
T4092 88-98 NNS denotes substrates
T4093 98-288 sentence denotes The above results suggest that Mcm4/6/7 generates a double hexameric complex on a bubble, each hexamer of which makes symmetric contact with the 5′-proximal central single-stranded segment.
T4094 99-102 DT denotes The
T4095 109-116 NNS denotes results
T4096 103-108 JJ denotes above
T4097 117-124 VBP denotes suggest
T4098 125-129 IN denotes that
T4099 139-148 VBZ denotes generates
T4100 130-134 NN denotes Mcm4
T4101 134-135 HYPH denotes /
T4102 135-136 CD denotes 6
T4103 136-137 HYPH denotes /
T4104 137-138 CD denotes 7
T4105 149-150 DT denotes a
T4106 168-175 NN denotes complex
T4107 151-157 JJ denotes double
T4108 158-167 JJ denotes hexameric
T4109 176-178 IN denotes on
T4110 179-180 DT denotes a
T4111 181-187 NN denotes bubble
T4112 187-189 , denotes ,
T4113 189-193 DT denotes each
T4114 194-201 NN denotes hexamer
T4115 211-216 VBZ denotes makes
T4116 202-204 IN denotes of
T4117 205-210 WDT denotes which
T4118 217-226 JJ denotes symmetric
T4119 227-234 NN denotes contact
T4120 235-239 IN denotes with
T4121 240-243 DT denotes the
T4122 280-287 NN denotes segment
T4123 244-245 CD denotes 5
T4124 247-255 JJ denotes proximal
T4125 245-246 SYM denotes ′
T4126 246-247 HYPH denotes -
T4127 256-263 JJ denotes central
T4128 264-270 JJ denotes single
T4129 271-279 VBN denotes stranded
T4130 270-271 HYPH denotes -
T4131 287-288 . denotes .
T4132 288-411 sentence denotes This predicts that the single-stranded segment needs to be of a sufficient length in order to accommodate the Mcm complex.
T4133 289-293 DT denotes This
T4134 294-302 VBZ denotes predicts
T4135 303-307 IN denotes that
T4136 336-341 VBZ denotes needs
T4137 308-311 DT denotes the
T4138 328-335 NN denotes segment
T4139 312-318 JJ denotes single
T4140 319-327 VBN denotes stranded
T4141 318-319 HYPH denotes -
T4142 342-344 TO denotes to
T4143 345-347 VB denotes be
T4144 348-350 IN denotes of
T4145 351-352 DT denotes a
T4146 364-370 NN denotes length
T4147 353-363 JJ denotes sufficient
T4148 371-373 IN denotes in
T4149 374-379 NN denotes order
T4150 380-382 TO denotes to
T4151 383-394 VB denotes accommodate
T4152 395-398 DT denotes the
T4153 403-410 NN denotes complex
T4154 399-402 NN denotes Mcm
T4155 410-411 . denotes .
T4156 411-635 sentence denotes In fact, we previously showed that the Mcm4/6/7 mainly forms a single hexameric complex on bubble-20 (a bubble substrate with 20 nt long central single-stranded segment) and a double hexameric complex on bubble-60 DNA (13).
T4157 412-414 IN denotes In
T4158 435-441 VBD denotes showed
T4159 415-419 NN denotes fact
T4160 419-421 , denotes ,
T4161 421-423 PRP denotes we
T4162 424-434 RB denotes previously
T4163 442-446 IN denotes that
T4164 467-472 VBZ denotes forms
T4165 447-450 DT denotes the
T4166 451-455 NN denotes Mcm4
T4167 455-456 HYPH denotes /
T4168 456-457 CD denotes 6
T4169 457-458 HYPH denotes /
T4170 458-459 CD denotes 7
T4171 460-466 RB denotes mainly
T4172 473-474 DT denotes a
T4173 492-499 NN denotes complex
T4174 475-481 JJ denotes single
T4175 482-491 JJ denotes hexameric
T4176 500-502 IN denotes on
T4177 503-509 NN denotes bubble
T4178 509-510 HYPH denotes -
T4179 510-512 CD denotes 20
T4180 513-514 -LRB- denotes (
T4181 514-515 DT denotes a
T4182 523-532 NN denotes substrate
T4183 516-522 NN denotes bubble
T4184 533-537 IN denotes with
T4185 538-540 CD denotes 20
T4186 541-543 NN denotes nt
T4187 544-548 JJ denotes long
T4188 573-580 NN denotes segment
T4189 549-556 JJ denotes central
T4190 557-563 JJ denotes single
T4191 564-572 VBN denotes stranded
T4192 563-564 HYPH denotes -
T4193 580-581 -RRB- denotes )
T4194 582-585 CC denotes and
T4195 586-587 DT denotes a
T4196 605-612 NN denotes complex
T4197 588-594 JJ denotes double
T4198 595-604 JJ denotes hexameric
T4199 613-615 IN denotes on
T4200 616-622 NN denotes bubble
T4201 626-629 NN denotes DNA
T4202 622-623 HYPH denotes -
T4203 623-625 CD denotes 60
T4204 630-631 -LRB- denotes (
T4205 631-633 CD denotes 13
T4206 633-634 -RRB- denotes )
T4207 634-635 . denotes .
T4208 635-967 sentence denotes In order to determine the minimum requirement for double-hexamer formation, a set of synthetic bubble-like substrates (Bub-T10, Bub-T20, Bub-T30, Bub-T40 and Bub-T50) that differed in the length of the central unpaired segment were constructed, and the binding and helicase activities of Mcm4/6/7 on these substrates were examined.
T4209 636-638 IN denotes In
T4210 868-879 VBN denotes constructed
T4211 639-644 NN denotes order
T4212 645-647 TO denotes to
T4213 648-657 VB denotes determine
T4214 658-661 DT denotes the
T4215 670-681 NN denotes requirement
T4216 662-669 JJ denotes minimum
T4217 682-685 IN denotes for
T4218 686-692 JJ denotes double
T4219 693-700 NN denotes hexamer
T4220 692-693 HYPH denotes -
T4221 701-710 NN denotes formation
T4222 710-712 , denotes ,
T4223 712-713 DT denotes a
T4224 714-717 NN denotes set
T4225 718-720 IN denotes of
T4226 721-730 JJ denotes synthetic
T4227 743-753 NNS denotes substrates
T4228 731-737 NN denotes bubble
T4229 738-742 JJ denotes like
T4230 737-738 HYPH denotes -
T4231 754-755 -LRB- denotes (
T4232 755-758 NN denotes Bub
T4233 759-762 NN denotes T10
T4234 758-759 HYPH denotes -
T4235 762-764 , denotes ,
T4236 764-767 NN denotes Bub
T4237 768-771 NN denotes T20
T4238 767-768 HYPH denotes -
T4239 771-773 , denotes ,
T4240 773-776 NN denotes Bub
T4241 777-780 NN denotes T30
T4242 776-777 HYPH denotes -
T4243 780-782 , denotes ,
T4244 782-785 NN denotes Bub
T4245 786-789 NN denotes T40
T4246 785-786 HYPH denotes -
T4247 790-793 CC denotes and
T4248 794-797 NN denotes Bub
T4249 798-801 NN denotes T50
T4250 797-798 HYPH denotes -
T4251 801-802 -RRB- denotes )
T4252 803-807 WDT denotes that
T4253 808-816 VBD denotes differed
T4254 817-819 IN denotes in
T4255 820-823 DT denotes the
T4256 824-830 NN denotes length
T4257 831-833 IN denotes of
T4258 834-837 DT denotes the
T4259 855-862 NN denotes segment
T4260 838-845 JJ denotes central
T4261 846-854 JJ denotes unpaired
T4262 863-867 VBD denotes were
T4263 879-881 , denotes ,
T4264 881-884 CC denotes and
T4265 885-888 DT denotes the
T4266 910-920 NNS denotes activities
T4267 889-896 NN denotes binding
T4268 897-900 CC denotes and
T4269 901-909 NN denotes helicase
T4270 958-966 VBN denotes examined
T4271 921-923 IN denotes of
T4272 924-928 NN denotes Mcm4
T4273 928-929 HYPH denotes /
T4274 929-930 CD denotes 6
T4275 930-931 HYPH denotes /
T4276 931-932 CD denotes 7
T4277 933-935 IN denotes on
T4278 936-941 DT denotes these
T4279 942-952 NNS denotes substrates
T4280 953-957 VBD denotes were
T4281 966-967 . denotes .
T4282 967-1099 sentence denotes Mcm4/6/7 was incubated with a radiolabeled synthetic bubble substrate in the presence of 0.5 mM ATP-γ-S to allow complex formation.
T4283 968-972 NN denotes Mcm4
T4284 981-990 VBN denotes incubated
T4285 972-973 HYPH denotes /
T4286 973-974 CD denotes 6
T4287 974-975 HYPH denotes /
T4288 975-976 CD denotes 7
T4289 977-980 VBD denotes was
T4290 991-995 IN denotes with
T4291 996-997 DT denotes a
T4292 1028-1037 NN denotes substrate
T4293 998-1010 VBN denotes radiolabeled
T4294 1011-1020 JJ denotes synthetic
T4295 1021-1027 NN denotes bubble
T4296 1038-1040 IN denotes in
T4297 1041-1044 DT denotes the
T4298 1045-1053 NN denotes presence
T4299 1054-1056 IN denotes of
T4300 1057-1060 CD denotes 0.5
T4301 1061-1063 NN denotes mM
T4302 1070-1071 NN denotes S
T4303 1064-1067 NN denotes ATP
T4304 1067-1068 HYPH denotes -
T4305 1068-1069 SYM denotes γ
T4306 1069-1070 HYPH denotes -
T4307 1072-1074 TO denotes to
T4308 1075-1080 VB denotes allow
T4309 1081-1088 NN denotes complex
T4310 1089-1098 NN denotes formation
T4311 1098-1099 . denotes .
T4312 1099-1311 sentence denotes A half of these reaction mixtures were analyzed for DNA binding in gel shift assays (Figure 2A), and the remainder was further incubated in the presence of 10 mM ATP to measure DNA helicase activity (Figure 2B).
T4313 1100-1101 DT denotes A
T4314 1102-1106 NN denotes half
T4315 1139-1147 VBN denotes analyzed
T4316 1107-1109 IN denotes of
T4317 1110-1115 DT denotes these
T4318 1125-1133 NNS denotes mixtures
T4319 1116-1124 NN denotes reaction
T4320 1134-1138 VBD denotes were
T4321 1148-1151 IN denotes for
T4322 1152-1155 NN denotes DNA
T4323 1156-1163 NN denotes binding
T4324 1164-1166 IN denotes in
T4325 1167-1170 NN denotes gel
T4326 1171-1176 NN denotes shift
T4327 1177-1183 NNS denotes assays
T4328 1184-1185 -LRB- denotes (
T4329 1192-1194 NN denotes 2A
T4330 1185-1191 NN denotes Figure
T4331 1194-1195 -RRB- denotes )
T4332 1195-1197 , denotes ,
T4333 1197-1200 CC denotes and
T4334 1201-1204 DT denotes the
T4335 1205-1214 NN denotes remainder
T4336 1227-1236 VBN denotes incubated
T4337 1215-1218 VBD denotes was
T4338 1219-1226 RB denotes further
T4339 1237-1239 IN denotes in
T4340 1240-1243 DT denotes the
T4341 1244-1252 NN denotes presence
T4342 1253-1255 IN denotes of
T4343 1256-1258 CD denotes 10
T4344 1259-1261 NN denotes mM
T4345 1262-1265 NN denotes ATP
T4346 1266-1268 TO denotes to
T4347 1269-1276 VB denotes measure
T4348 1277-1280 NN denotes DNA
T4349 1281-1289 NN denotes helicase
T4350 1290-1298 NN denotes activity
T4351 1299-1300 -LRB- denotes (
T4352 1307-1309 NN denotes 2B
T4353 1300-1306 NN denotes Figure
T4354 1309-1310 -RRB- denotes )
T4355 1310-1311 . denotes .
T4356 1311-1484 sentence denotes Bub-10 generated only a low level of complexes with Mcm4/6/7, and Bub-20 generated primarily a single mobility-shifted form, which may contain a single hexamer of Mcm4/6/7.
T4357 1312-1315 NN denotes Bub
T4358 1319-1328 VBD denotes generated
T4359 1315-1316 HYPH denotes -
T4360 1316-1318 CD denotes 10
T4361 1329-1333 RB denotes only
T4362 1340-1345 NN denotes level
T4363 1334-1335 DT denotes a
T4364 1336-1339 JJ denotes low
T4365 1346-1348 IN denotes of
T4366 1349-1358 NNS denotes complexes
T4367 1359-1363 IN denotes with
T4368 1364-1368 NN denotes Mcm4
T4369 1368-1369 HYPH denotes /
T4370 1369-1370 CD denotes 6
T4371 1370-1371 HYPH denotes /
T4372 1371-1372 CD denotes 7
T4373 1372-1374 , denotes ,
T4374 1374-1377 CC denotes and
T4375 1378-1381 NN denotes Bub
T4376 1385-1394 VBD denotes generated
T4377 1381-1382 HYPH denotes -
T4378 1382-1384 CD denotes 20
T4379 1395-1404 RB denotes primarily
T4380 1405-1406 DT denotes a
T4381 1431-1435 NN denotes form
T4382 1407-1413 JJ denotes single
T4383 1414-1422 NN denotes mobility
T4384 1423-1430 VBN denotes shifted
T4385 1422-1423 HYPH denotes -
T4386 1435-1437 , denotes ,
T4387 1437-1442 WDT denotes which
T4388 1447-1454 VB denotes contain
T4389 1443-1446 MD denotes may
T4390 1455-1456 DT denotes a
T4391 1464-1471 NN denotes hexamer
T4392 1457-1463 JJ denotes single
T4393 1472-1474 IN denotes of
T4394 1475-1479 NN denotes Mcm4
T4395 1479-1480 HYPH denotes /
T4396 1480-1481 CD denotes 6
T4397 1481-1482 HYPH denotes /
T4398 1482-1483 CD denotes 7
T4399 1483-1484 . denotes .
T4400 1484-1640 sentence denotes On Bub-30, significant amount of complexes containing a double hexamer were generated in addition to those containing a single hexamer (Figure 2A) (13,21).
T4401 1485-1487 IN denotes On
T4402 1561-1570 VBN denotes generated
T4403 1488-1491 NN denotes Bub
T4404 1491-1492 HYPH denotes -
T4405 1492-1494 CD denotes 30
T4406 1494-1496 , denotes ,
T4407 1496-1507 JJ denotes significant
T4408 1508-1514 NN denotes amount
T4409 1515-1517 IN denotes of
T4410 1518-1527 NNS denotes complexes
T4411 1528-1538 VBG denotes containing
T4412 1539-1540 DT denotes a
T4413 1548-1555 NN denotes hexamer
T4414 1541-1547 JJ denotes double
T4415 1556-1560 VBD denotes were
T4416 1571-1573 IN denotes in
T4417 1574-1582 NN denotes addition
T4418 1583-1585 IN denotes to
T4419 1586-1591 DT denotes those
T4420 1592-1602 VBG denotes containing
T4421 1603-1604 DT denotes a
T4422 1612-1619 NN denotes hexamer
T4423 1605-1611 JJ denotes single
T4424 1620-1621 -LRB- denotes (
T4425 1628-1630 NN denotes 2A
T4426 1621-1627 NN denotes Figure
T4427 1630-1631 -RRB- denotes )
T4428 1632-1633 -LRB- denotes (
T4429 1636-1638 CD denotes 21
T4430 1633-1635 CD denotes 13
T4431 1635-1636 , denotes ,
T4432 1638-1639 -RRB- denotes )
T4433 1639-1640 . denotes .
T4434 1640-1780 sentence denotes The amounts of double hexamer complexes increased on Bub-T40 and Bub-T50 with concomitant decrease of single hexamer complexes (Figure 2A).
T4435 1641-1644 DT denotes The
T4436 1645-1652 NNS denotes amounts
T4437 1681-1690 VBD denotes increased
T4438 1653-1655 IN denotes of
T4439 1656-1662 JJ denotes double
T4440 1663-1670 NN denotes hexamer
T4441 1671-1680 NNS denotes complexes
T4442 1691-1693 IN denotes on
T4443 1694-1697 NN denotes Bub
T4444 1698-1701 NN denotes T40
T4445 1697-1698 HYPH denotes -
T4446 1702-1705 CC denotes and
T4447 1706-1709 NN denotes Bub
T4448 1710-1713 NN denotes T50
T4449 1709-1710 HYPH denotes -
T4450 1714-1718 IN denotes with
T4451 1719-1730 JJ denotes concomitant
T4452 1731-1739 NN denotes decrease
T4453 1740-1742 IN denotes of
T4454 1743-1749 JJ denotes single
T4455 1750-1757 NN denotes hexamer
T4456 1758-1767 NNS denotes complexes
T4457 1768-1769 -LRB- denotes (
T4458 1776-1778 NN denotes 2A
T4459 1769-1775 NN denotes Figure
T4460 1778-1779 -RRB- denotes )
T4461 1779-1780 . denotes .
T4462 1780-1949 sentence denotes The results indicate that a single-stranded segment of at least 40 nt long is required for efficient loading of the double-hexameric Mcm helicase on a bubble substrate.
T4463 1781-1784 DT denotes The
T4464 1785-1792 NNS denotes results
T4465 1793-1801 VBP denotes indicate
T4466 1802-1806 IN denotes that
T4467 1859-1867 VBN denotes required
T4468 1807-1808 DT denotes a
T4469 1825-1832 NN denotes segment
T4470 1809-1815 JJ denotes single
T4471 1816-1824 VBN denotes stranded
T4472 1815-1816 HYPH denotes -
T4473 1833-1835 IN denotes of
T4474 1836-1838 RB denotes at
T4475 1839-1844 RBS denotes least
T4476 1845-1847 CD denotes 40
T4477 1848-1850 NN denotes nt
T4478 1851-1855 JJ denotes long
T4479 1856-1858 VBZ denotes is
T4480 1868-1871 IN denotes for
T4481 1872-1881 JJ denotes efficient
T4482 1882-1889 NN denotes loading
T4483 1890-1892 IN denotes of
T4484 1893-1896 DT denotes the
T4485 1918-1926 NN denotes helicase
T4486 1897-1903 JJ denotes double
T4487 1904-1913 JJ denotes hexameric
T4488 1903-1904 HYPH denotes -
T4489 1914-1917 NN denotes Mcm
T4490 1927-1929 IN denotes on
T4491 1930-1931 DT denotes a
T4492 1939-1948 NN denotes substrate
T4493 1932-1938 NN denotes bubble
T4494 1948-1949 . denotes .
T4495 1949-2059 sentence denotes In DNA helicase assays, Mcm4/6/7 complex did not show helicase activity on either Bub-10 or Bub-20 substrate.
T4496 1950-1952 IN denotes In
T4497 1999-2003 VB denotes show
T4498 1953-1956 NN denotes DNA
T4499 1957-1965 NN denotes helicase
T4500 1966-1972 NNS denotes assays
T4501 1972-1974 , denotes ,
T4502 1974-1978 NN denotes Mcm4
T4503 1983-1990 NN denotes complex
T4504 1978-1979 HYPH denotes /
T4505 1979-1980 CD denotes 6
T4506 1980-1981 HYPH denotes /
T4507 1981-1982 CD denotes 7
T4508 1991-1994 VBD denotes did
T4509 1995-1998 RB denotes not
T4510 2004-2012 NN denotes helicase
T4511 2013-2021 NN denotes activity
T4512 2022-2024 IN denotes on
T4513 2025-2031 CC denotes either
T4514 2032-2035 NN denotes Bub
T4515 2049-2058 NN denotes substrate
T4516 2035-2036 HYPH denotes -
T4517 2036-2038 CD denotes 10
T4518 2039-2041 CC denotes or
T4519 2042-2045 NN denotes Bub
T4520 2045-2046 HYPH denotes -
T4521 2046-2048 CD denotes 20
T4522 2058-2059 . denotes .
T4523 2059-2176 sentence denotes The efficiency of displacement slightly improved as the length of the single-stranded segment increased up to 30 nt.
T4524 2060-2063 DT denotes The
T4525 2064-2074 NN denotes efficiency
T4526 2100-2108 VBD denotes improved
T4527 2075-2077 IN denotes of
T4528 2078-2090 NN denotes displacement
T4529 2091-2099 RB denotes slightly
T4530 2109-2111 IN denotes as
T4531 2154-2163 VBD denotes increased
T4532 2112-2115 DT denotes the
T4533 2116-2122 NN denotes length
T4534 2123-2125 IN denotes of
T4535 2126-2129 DT denotes the
T4536 2146-2153 NN denotes segment
T4537 2130-2136 JJ denotes single
T4538 2137-2145 VBN denotes stranded
T4539 2136-2137 HYPH denotes -
T4540 2164-2166 IN denotes up
T4541 2170-2172 CD denotes 30
T4542 2167-2169 IN denotes to
T4543 2173-2175 NN denotes nt
T4544 2175-2176 . denotes .
T4545 2176-2400 sentence denotes On Bub-T40 and Bub-T50, on the other hand, significant displacement was observed, indicating that a single-stranded segment of at least 40 nt long is required for efficient helicase actions on bubble substrates (Figure 2B).
T4546 2177-2179 IN denotes On
T4547 2249-2257 VBN denotes observed
T4548 2180-2183 NN denotes Bub
T4549 2184-2187 NN denotes T40
T4550 2183-2184 HYPH denotes -
T4551 2188-2191 CC denotes and
T4552 2192-2195 NN denotes Bub
T4553 2196-2199 NN denotes T50
T4554 2195-2196 HYPH denotes -
T4555 2199-2201 , denotes ,
T4556 2201-2203 IN denotes on
T4557 2204-2207 DT denotes the
T4558 2214-2218 NN denotes hand
T4559 2208-2213 JJ denotes other
T4560 2218-2220 , denotes ,
T4561 2220-2231 JJ denotes significant
T4562 2232-2244 NN denotes displacement
T4563 2245-2248 VBD denotes was
T4564 2257-2259 , denotes ,
T4565 2259-2269 VBG denotes indicating
T4566 2270-2274 IN denotes that
T4567 2327-2335 VBN denotes required
T4568 2275-2276 DT denotes a
T4569 2293-2300 NN denotes segment
T4570 2277-2283 JJ denotes single
T4571 2284-2292 VBN denotes stranded
T4572 2283-2284 HYPH denotes -
T4573 2301-2303 IN denotes of
T4574 2304-2306 RB denotes at
T4575 2307-2312 RBS denotes least
T4576 2313-2315 CD denotes 40
T4577 2316-2318 NN denotes nt
T4578 2319-2323 JJ denotes long
T4579 2324-2326 VBZ denotes is
T4580 2336-2339 IN denotes for
T4581 2340-2349 JJ denotes efficient
T4582 2359-2366 NNS denotes actions
T4583 2350-2358 NN denotes helicase
T4584 2367-2369 IN denotes on
T4585 2370-2376 NN denotes bubble
T4586 2377-2387 NNS denotes substrates
T4587 2388-2389 -LRB- denotes (
T4588 2396-2398 NN denotes 2B
T4589 2389-2395 NN denotes Figure
T4590 2398-2399 -RRB- denotes )
T4591 2399-2400 . denotes .
T4592 2400-2599 sentence denotes Taken together, the above results are consistent with the notion that the Mcm4/6/7 complex, loaded onto DNA as a double hexameric complex, efficiently unwinds the duplex region of the substrate DNA.
T4593 2401-2406 VBN denotes Taken
T4594 2435-2438 VBP denotes are
T4595 2407-2415 RB denotes together
T4596 2415-2417 , denotes ,
T4597 2417-2420 DT denotes the
T4598 2427-2434 NNS denotes results
T4599 2421-2426 JJ denotes above
T4600 2439-2449 JJ denotes consistent
T4601 2450-2454 IN denotes with
T4602 2455-2458 DT denotes the
T4603 2459-2465 NN denotes notion
T4604 2466-2470 IN denotes that
T4605 2552-2559 VBZ denotes unwinds
T4606 2471-2474 DT denotes the
T4607 2484-2491 NN denotes complex
T4608 2475-2479 NN denotes Mcm4
T4609 2479-2480 HYPH denotes /
T4610 2480-2481 CD denotes 6
T4611 2481-2482 HYPH denotes /
T4612 2482-2483 CD denotes 7
T4613 2491-2493 , denotes ,
T4614 2493-2499 VBN denotes loaded
T4615 2500-2504 IN denotes onto
T4616 2505-2508 NN denotes DNA
T4617 2509-2511 IN denotes as
T4618 2512-2513 DT denotes a
T4619 2531-2538 NN denotes complex
T4620 2514-2520 JJ denotes double
T4621 2521-2530 JJ denotes hexameric
T4622 2538-2540 , denotes ,
T4623 2540-2551 RB denotes efficiently
T4624 2560-2563 DT denotes the
T4625 2571-2577 NN denotes region
T4626 2564-2570 NN denotes duplex
T4627 2578-2580 IN denotes of
T4628 2581-2584 DT denotes the
T4629 2595-2598 NN denotes DNA
T4630 2585-2594 NN denotes substrate
T4631 2598-2599 . denotes .
R2617 T4077 T4078 nsubj Formation,facilitates
R2618 T4079 T4077 prep of,Formation
R2619 T4080 T4081 amod double,hexameric
R2620 T4081 T4082 amod hexameric,complexes
R2621 T4082 T4079 pobj complexes,of
R2622 T4083 T4082 nmod Mcm4,complexes
R2623 T4084 T4083 punct /,Mcm4
R2624 T4085 T4083 nummod 6,Mcm4
R2625 T4086 T4083 punct /,Mcm4
R2626 T4087 T4083 nummod 7,Mcm4
R2627 T4088 T4089 compound helicase,actions
R2628 T4089 T4078 dobj actions,facilitates
R2629 T4090 T4078 prep on,facilitates
R2630 T4091 T4092 compound bubble,substrates
R2631 T4092 T4090 pobj substrates,on
R2632 T4094 T4095 det The,results
R2633 T4095 T4097 nsubj results,suggest
R2634 T4096 T4095 amod above,results
R2635 T4098 T4099 mark that,generates
R2636 T4099 T4097 ccomp generates,suggest
R2637 T4100 T4099 nsubj Mcm4,generates
R2638 T4101 T4100 punct /,Mcm4
R2639 T4102 T4100 nummod 6,Mcm4
R2640 T4103 T4100 punct /,Mcm4
R2641 T4104 T4100 nummod 7,Mcm4
R2642 T4105 T4106 det a,complex
R2643 T4106 T4099 dobj complex,generates
R2644 T4107 T4108 amod double,hexameric
R2645 T4108 T4106 amod hexameric,complex
R2646 T4109 T4099 prep on,generates
R2647 T4110 T4111 det a,bubble
R2648 T4111 T4109 pobj bubble,on
R2649 T4112 T4099 punct ", ",generates
R2650 T4113 T4114 det each,hexamer
R2651 T4114 T4115 dep hexamer,makes
R2652 T4115 T4099 ccomp makes,generates
R2653 T4116 T4114 prep of,hexamer
R2654 T4117 T4116 pobj which,of
R2655 T4118 T4119 amod symmetric,contact
R2656 T4119 T4115 dobj contact,makes
R2657 T4120 T4119 prep with,contact
R2658 T4121 T4122 det the,segment
R2659 T4122 T4120 pobj segment,with
R2660 T4123 T4124 npadvmod 5,proximal
R2661 T4124 T4122 amod proximal,segment
R2662 T4125 T4123 punct ′,5
R2663 T4126 T4124 punct -,proximal
R2664 T4127 T4122 amod central,segment
R2665 T4128 T4129 amod single,stranded
R2666 T4129 T4122 amod stranded,segment
R2667 T4130 T4129 punct -,stranded
R2668 T4131 T4097 punct .,suggest
R2669 T4133 T4134 nsubj This,predicts
R2670 T4135 T4136 mark that,needs
R2671 T4136 T4134 ccomp needs,predicts
R2672 T4137 T4138 det the,segment
R2673 T4138 T4136 nsubj segment,needs
R2674 T4139 T4140 amod single,stranded
R2675 T4140 T4138 amod stranded,segment
R2676 T4141 T4140 punct -,stranded
R2677 T4142 T4143 aux to,be
R2678 T4143 T4136 xcomp be,needs
R2679 T4144 T4143 prep of,be
R2680 T4145 T4146 det a,length
R2681 T4146 T4144 pobj length,of
R2682 T4147 T4146 amod sufficient,length
R2683 T4148 T4143 prep in,be
R2684 T4149 T4148 pobj order,in
R2685 T4150 T4151 aux to,accommodate
R2686 T4151 T4149 acl accommodate,order
R2687 T4152 T4153 det the,complex
R2688 T4153 T4151 dobj complex,accommodate
R2689 T4154 T4153 compound Mcm,complex
R2690 T4155 T4134 punct .,predicts
R2691 T4157 T4158 prep In,showed
R2692 T4159 T4157 pobj fact,In
R2693 T4160 T4158 punct ", ",showed
R2694 T4161 T4158 nsubj we,showed
R2695 T4162 T4158 advmod previously,showed
R2696 T4163 T4164 mark that,forms
R2697 T4164 T4158 ccomp forms,showed
R2698 T4165 T4166 det the,Mcm4
R2699 T4166 T4164 nsubj Mcm4,forms
R2700 T4167 T4166 punct /,Mcm4
R2701 T4168 T4166 nummod 6,Mcm4
R2702 T4169 T4166 punct /,Mcm4
R2703 T4170 T4166 nummod 7,Mcm4
R2704 T4171 T4164 advmod mainly,forms
R2705 T4172 T4173 det a,complex
R2706 T4173 T4164 dobj complex,forms
R2707 T4174 T4175 amod single,hexameric
R2708 T4175 T4173 amod hexameric,complex
R2709 T4176 T4173 prep on,complex
R2710 T4177 T4176 pobj bubble,on
R2711 T4178 T4177 punct -,bubble
R2712 T4179 T4177 nummod 20,bubble
R2713 T4180 T4177 punct (,bubble
R2714 T4181 T4182 det a,substrate
R2715 T4182 T4177 appos substrate,bubble
R2716 T4183 T4182 compound bubble,substrate
R2717 T4184 T4182 prep with,substrate
R2718 T4185 T4186 nummod 20,nt
R2719 T4186 T4187 npadvmod nt,long
R2720 T4187 T4188 amod long,segment
R2721 T4188 T4184 pobj segment,with
R2722 T4189 T4188 amod central,segment
R2723 T4190 T4191 amod single,stranded
R2724 T4191 T4188 amod stranded,segment
R2725 T4192 T4191 punct -,stranded
R2726 T4193 T4173 punct ),complex
R2727 T4194 T4173 cc and,complex
R2728 T4195 T4196 det a,complex
R2729 T4196 T4173 conj complex,complex
R2730 T4197 T4198 amod double,hexameric
R2731 T4198 T4196 amod hexameric,complex
R2732 T4199 T4196 prep on,complex
R2733 T4200 T4201 nmod bubble,DNA
R2734 T4201 T4199 pobj DNA,on
R2735 T4202 T4200 punct -,bubble
R2736 T4203 T4200 nummod 60,bubble
R2737 T4204 T4205 punct (,13
R2738 T4205 T4196 parataxis 13,complex
R2739 T4206 T4205 punct ),13
R2740 T4207 T4158 punct .,showed
R2741 T4209 T4210 prep In,constructed
R2742 T4211 T4209 pobj order,In
R2743 T4212 T4213 aux to,determine
R2744 T4213 T4211 acl determine,order
R2745 T4214 T4215 det the,requirement
R2746 T4215 T4213 dobj requirement,determine
R2747 T4216 T4215 amod minimum,requirement
R2748 T4217 T4215 prep for,requirement
R2749 T4218 T4219 amod double,hexamer
R2750 T4219 T4221 compound hexamer,formation
R2751 T4220 T4219 punct -,hexamer
R2752 T4221 T4217 pobj formation,for
R2753 T4222 T4210 punct ", ",constructed
R2754 T4223 T4224 det a,set
R2755 T4224 T4210 nsubjpass set,constructed
R2756 T4225 T4224 prep of,set
R2757 T4226 T4227 amod synthetic,substrates
R2758 T4227 T4225 pobj substrates,of
R2759 T4228 T4229 npadvmod bubble,like
R2760 T4229 T4227 amod like,substrates
R2761 T4230 T4229 punct -,like
R2762 T4231 T4227 punct (,substrates
R2763 T4232 T4233 compound Bub,T10
R2764 T4233 T4227 appos T10,substrates
R2765 T4234 T4233 punct -,T10
R2766 T4235 T4233 punct ", ",T10
R2767 T4236 T4237 compound Bub,T20
R2768 T4237 T4233 conj T20,T10
R2769 T4238 T4237 punct -,T20
R2770 T4239 T4237 punct ", ",T20
R2771 T4240 T4241 compound Bub,T30
R2772 T4241 T4237 conj T30,T20
R2773 T4242 T4241 punct -,T30
R2774 T4243 T4241 punct ", ",T30
R2775 T4244 T4245 compound Bub,T40
R2776 T4245 T4241 conj T40,T30
R2777 T4246 T4245 punct -,T40
R2778 T4247 T4245 cc and,T40
R2779 T4248 T4249 compound Bub,T50
R2780 T4249 T4245 conj T50,T40
R2781 T4250 T4249 punct -,T50
R2782 T4251 T4227 punct ),substrates
R2783 T4252 T4253 dep that,differed
R2784 T4253 T4227 relcl differed,substrates
R2785 T4254 T4253 prep in,differed
R2786 T4255 T4256 det the,length
R2787 T4256 T4254 pobj length,in
R2788 T4257 T4256 prep of,length
R2789 T4258 T4259 det the,segment
R2790 T4259 T4257 pobj segment,of
R2791 T4260 T4259 amod central,segment
R2792 T4261 T4259 amod unpaired,segment
R2793 T4262 T4210 auxpass were,constructed
R2794 T4263 T4210 punct ", ",constructed
R2795 T4264 T4210 cc and,constructed
R2796 T4265 T4266 det the,activities
R2797 T4266 T4270 nsubjpass activities,examined
R2798 T4267 T4266 nmod binding,activities
R2799 T4268 T4267 cc and,binding
R2800 T4269 T4267 conj helicase,binding
R2801 T4270 T4210 conj examined,constructed
R2802 T4271 T4266 prep of,activities
R2803 T4272 T4271 pobj Mcm4,of
R2804 T4273 T4272 punct /,Mcm4
R2805 T4274 T4272 nummod 6,Mcm4
R2806 T4275 T4272 punct /,Mcm4
R2807 T4276 T4272 nummod 7,Mcm4
R2808 T4277 T4266 prep on,activities
R2809 T4278 T4279 det these,substrates
R2810 T4279 T4277 pobj substrates,on
R2811 T4280 T4270 auxpass were,examined
R2812 T4281 T4210 punct .,constructed
R2813 T4283 T4284 nsubjpass Mcm4,incubated
R2814 T4285 T4283 punct /,Mcm4
R2815 T4286 T4283 nummod 6,Mcm4
R2816 T4287 T4283 punct /,Mcm4
R2817 T4288 T4283 nummod 7,Mcm4
R2818 T4289 T4284 auxpass was,incubated
R2819 T4290 T4284 prep with,incubated
R2820 T4291 T4292 det a,substrate
R2821 T4292 T4290 pobj substrate,with
R2822 T4293 T4292 amod radiolabeled,substrate
R2823 T4294 T4292 amod synthetic,substrate
R2824 T4295 T4292 compound bubble,substrate
R2825 T4296 T4284 prep in,incubated
R2826 T4297 T4298 det the,presence
R2827 T4298 T4296 pobj presence,in
R2828 T4299 T4298 prep of,presence
R2829 T4300 T4301 nummod 0.5,mM
R2830 T4301 T4302 nmod mM,S
R2831 T4302 T4299 pobj S,of
R2832 T4303 T4302 nmod ATP,S
R2833 T4304 T4302 punct -,S
R2834 T4305 T4302 punct γ,S
R2835 T4306 T4302 punct -,S
R2836 T4307 T4308 aux to,allow
R2837 T4308 T4284 advcl allow,incubated
R2838 T4309 T4310 compound complex,formation
R2839 T4310 T4308 dobj formation,allow
R2840 T4311 T4284 punct .,incubated
R2841 T4313 T4314 det A,half
R2842 T4314 T4315 nsubjpass half,analyzed
R2843 T4316 T4314 prep of,half
R2844 T4317 T4318 det these,mixtures
R2845 T4318 T4316 pobj mixtures,of
R2846 T4319 T4318 compound reaction,mixtures
R2847 T4320 T4315 auxpass were,analyzed
R2848 T4321 T4315 prep for,analyzed
R2849 T4322 T4323 compound DNA,binding
R2850 T4323 T4321 pobj binding,for
R2851 T4324 T4315 prep in,analyzed
R2852 T4325 T4326 compound gel,shift
R2853 T4326 T4327 compound shift,assays
R2854 T4327 T4324 pobj assays,in
R2855 T4328 T4329 punct (,2A
R2856 T4329 T4315 parataxis 2A,analyzed
R2857 T4330 T4329 compound Figure,2A
R2858 T4331 T4329 punct ),2A
R2859 T4332 T4315 punct ", ",analyzed
R2860 T4333 T4315 cc and,analyzed
R2861 T4334 T4335 det the,remainder
R2862 T4335 T4336 nsubjpass remainder,incubated
R2863 T4336 T4315 conj incubated,analyzed
R2864 T4337 T4336 auxpass was,incubated
R2865 T4338 T4336 advmod further,incubated
R2866 T4339 T4336 prep in,incubated
R2867 T4340 T4341 det the,presence
R2868 T4341 T4339 pobj presence,in
R2869 T4342 T4341 prep of,presence
R2870 T4343 T4344 nummod 10,mM
R2871 T4344 T4345 compound mM,ATP
R2872 T4345 T4342 pobj ATP,of
R2873 T4346 T4347 aux to,measure
R2874 T4347 T4336 advcl measure,incubated
R2875 T4348 T4349 compound DNA,helicase
R2876 T4349 T4350 compound helicase,activity
R2877 T4350 T4347 dobj activity,measure
R2878 T4351 T4352 punct (,2B
R2879 T4352 T4347 parataxis 2B,measure
R2880 T4353 T4352 compound Figure,2B
R2881 T4354 T4352 punct ),2B
R2882 T4355 T4336 punct .,incubated
R2883 T4357 T4358 nsubj Bub,generated
R2884 T4359 T4357 punct -,Bub
R2885 T4360 T4357 nummod 10,Bub
R2886 T4361 T4362 advmod only,level
R2887 T4362 T4358 dobj level,generated
R2888 T4363 T4362 det a,level
R2889 T4364 T4362 amod low,level
R2890 T4365 T4362 prep of,level
R2891 T4366 T4365 pobj complexes,of
R2892 T4367 T4366 prep with,complexes
R2893 T4368 T4367 pobj Mcm4,with
R2894 T4369 T4368 punct /,Mcm4
R2895 T4370 T4368 nummod 6,Mcm4
R2896 T4371 T4368 punct /,Mcm4
R2897 T4372 T4368 nummod 7,Mcm4
R2898 T4373 T4358 punct ", ",generated
R2899 T4374 T4358 cc and,generated
R2900 T4375 T4376 nsubj Bub,generated
R2901 T4376 T4358 conj generated,generated
R2902 T4377 T4375 punct -,Bub
R2903 T4378 T4375 nummod 20,Bub
R2904 T4379 T4376 advmod primarily,generated
R2905 T4380 T4381 det a,form
R2906 T4381 T4376 dobj form,generated
R2907 T4382 T4381 amod single,form
R2908 T4383 T4384 npadvmod mobility,shifted
R2909 T4384 T4381 amod shifted,form
R2910 T4385 T4384 punct -,shifted
R2911 T4386 T4381 punct ", ",form
R2912 T4387 T4388 dep which,contain
R2913 T4388 T4381 relcl contain,form
R2914 T4389 T4388 aux may,contain
R2915 T4390 T4391 det a,hexamer
R2916 T4391 T4388 dobj hexamer,contain
R2917 T4392 T4391 amod single,hexamer
R2918 T4393 T4391 prep of,hexamer
R2919 T4394 T4393 pobj Mcm4,of
R2920 T4395 T4394 punct /,Mcm4
R2921 T4396 T4394 nummod 6,Mcm4
R2922 T4397 T4394 punct /,Mcm4
R2923 T4398 T4394 nummod 7,Mcm4
R2924 T4399 T4376 punct .,generated
R2925 T4401 T4402 prep On,generated
R2926 T4403 T4401 pobj Bub,On
R2927 T4404 T4403 punct -,Bub
R2928 T4405 T4403 nummod 30,Bub
R2929 T4406 T4402 punct ", ",generated
R2930 T4407 T4408 amod significant,amount
R2931 T4408 T4402 nsubjpass amount,generated
R2932 T4409 T4408 prep of,amount
R2933 T4410 T4409 pobj complexes,of
R2934 T4411 T4410 acl containing,complexes
R2935 T4412 T4413 det a,hexamer
R2936 T4413 T4411 dobj hexamer,containing
R2937 T4414 T4413 amod double,hexamer
R2938 T4415 T4402 auxpass were,generated
R2939 T4416 T4402 prep in,generated
R2940 T4417 T4416 pobj addition,in
R2941 T4418 T4417 prep to,addition
R2942 T4419 T4418 pobj those,to
R2943 T4420 T4419 acl containing,those
R2944 T4421 T4422 det a,hexamer
R2945 T4422 T4420 dobj hexamer,containing
R2946 T4423 T4422 amod single,hexamer
R2947 T4424 T4425 punct (,2A
R2948 T4425 T4402 parataxis 2A,generated
R2949 T4426 T4425 compound Figure,2A
R2950 T4427 T4425 punct ),2A
R2951 T4428 T4429 punct (,21
R2952 T4429 T4402 parataxis 21,generated
R2953 T4430 T4429 nummod 13,21
R2954 T4431 T4429 punct ",",21
R2955 T4432 T4429 punct ),21
R2956 T4433 T4402 punct .,generated
R2957 T4435 T4436 det The,amounts
R2958 T4436 T4437 nsubj amounts,increased
R2959 T4438 T4436 prep of,amounts
R2960 T4439 T4440 amod double,hexamer
R2961 T4440 T4441 compound hexamer,complexes
R2962 T4441 T4438 pobj complexes,of
R2963 T4442 T4437 prep on,increased
R2964 T4443 T4444 compound Bub,T40
R2965 T4444 T4442 pobj T40,on
R2966 T4445 T4444 punct -,T40
R2967 T4446 T4444 cc and,T40
R2968 T4447 T4448 compound Bub,T50
R2969 T4448 T4444 conj T50,T40
R2970 T4449 T4448 punct -,T50
R2971 T4450 T4437 prep with,increased
R2972 T4451 T4452 amod concomitant,decrease
R2973 T4452 T4450 pobj decrease,with
R2974 T4453 T4452 prep of,decrease
R2975 T4454 T4455 amod single,hexamer
R2976 T4455 T4456 compound hexamer,complexes
R2977 T4456 T4453 pobj complexes,of
R2978 T4457 T4458 punct (,2A
R2979 T4458 T4437 parataxis 2A,increased
R2980 T4459 T4458 compound Figure,2A
R2981 T4460 T4458 punct ),2A
R2982 T4461 T4437 punct .,increased
R2983 T4463 T4464 det The,results
R2984 T4464 T4465 nsubj results,indicate
R2985 T4466 T4467 mark that,required
R2986 T4467 T4465 ccomp required,indicate
R2987 T4468 T4469 det a,segment
R2988 T4469 T4467 nsubjpass segment,required
R2989 T4470 T4471 amod single,stranded
R2990 T4471 T4469 amod stranded,segment
R2991 T4472 T4471 punct -,stranded
R2992 T4473 T4469 prep of,segment
R2993 T4474 T4475 advmod at,least
R2994 T4475 T4476 advmod least,40
R2995 T4476 T4477 nummod 40,nt
R2996 T4477 T4473 pobj nt,of
R2997 T4478 T4477 amod long,nt
R2998 T4479 T4467 auxpass is,required
R2999 T4480 T4467 prep for,required
R3000 T4481 T4482 amod efficient,loading
R3001 T4482 T4480 pobj loading,for
R3002 T4483 T4482 prep of,loading
R3003 T4484 T4485 det the,helicase
R3004 T4485 T4483 pobj helicase,of
R3005 T4486 T4487 amod double,hexameric
R3006 T4487 T4485 amod hexameric,helicase
R3007 T4488 T4487 punct -,hexameric
R3008 T4489 T4485 compound Mcm,helicase
R3009 T4490 T4482 prep on,loading
R3010 T4491 T4492 det a,substrate
R3011 T4492 T4490 pobj substrate,on
R3012 T4493 T4492 compound bubble,substrate
R3013 T4494 T4465 punct .,indicate
R3014 T4496 T4497 prep In,show
R3015 T4498 T4499 compound DNA,helicase
R3016 T4499 T4500 compound helicase,assays
R3017 T4500 T4496 pobj assays,In
R3018 T4501 T4497 punct ", ",show
R3019 T4502 T4503 nmod Mcm4,complex
R3020 T4503 T4497 nsubj complex,show
R3021 T4504 T4502 punct /,Mcm4
R3022 T4505 T4502 nummod 6,Mcm4
R3023 T4506 T4502 punct /,Mcm4
R3024 T4507 T4502 nummod 7,Mcm4
R3025 T4508 T4497 aux did,show
R3026 T4509 T4497 neg not,show
R3027 T4510 T4511 compound helicase,activity
R3028 T4511 T4497 dobj activity,show
R3029 T4512 T4497 prep on,show
R3030 T4513 T4514 preconj either,Bub
R3031 T4514 T4515 nmod Bub,substrate
R3032 T4515 T4512 pobj substrate,on
R3033 T4516 T4514 punct -,Bub
R3034 T4517 T4514 nummod 10,Bub
R3035 T4518 T4514 cc or,Bub
R3036 T4519 T4514 conj Bub,Bub
R3037 T4520 T4519 punct -,Bub
R3038 T4521 T4519 nummod 20,Bub
R3039 T4522 T4497 punct .,show
R3040 T4524 T4525 det The,efficiency
R3041 T4525 T4526 nsubj efficiency,improved
R3042 T4527 T4525 prep of,efficiency
R3043 T4528 T4527 pobj displacement,of
R3044 T4529 T4526 advmod slightly,improved
R3045 T4530 T4531 mark as,increased
R3046 T4531 T4526 advcl increased,improved
R3047 T4532 T4533 det the,length
R3048 T4533 T4531 nsubj length,increased
R3049 T4534 T4533 prep of,length
R3050 T4535 T4536 det the,segment
R3051 T4536 T4534 pobj segment,of
R3052 T4537 T4538 amod single,stranded
R3053 T4538 T4536 amod stranded,segment
R3054 T4539 T4538 punct -,stranded
R3055 T4540 T4541 quantmod up,30
R3056 T4541 T4543 nummod 30,nt
R3057 T4542 T4541 quantmod to,30
R3058 T4543 T4531 npadvmod nt,increased
R3059 T4544 T4526 punct .,improved
R3060 T4546 T4547 prep On,observed
R3061 T4548 T4549 compound Bub,T40
R3062 T4549 T4546 pobj T40,On
R3063 T4550 T4549 punct -,T40
R3064 T4551 T4549 cc and,T40
R3065 T4552 T4553 compound Bub,T50
R3066 T4553 T4549 conj T50,T40
R3067 T4554 T4553 punct -,T50
R3068 T4555 T4547 punct ", ",observed
R3069 T4556 T4547 prep on,observed
R3070 T4557 T4558 det the,hand
R3071 T4558 T4556 pobj hand,on
R3072 T4559 T4558 amod other,hand
R3073 T4560 T4547 punct ", ",observed
R3074 T4561 T4562 amod significant,displacement
R3075 T4562 T4547 nsubjpass displacement,observed
R3076 T4563 T4547 auxpass was,observed
R3077 T4564 T4547 punct ", ",observed
R3078 T4565 T4547 advcl indicating,observed
R3079 T4566 T4567 mark that,required
R3080 T4567 T4565 ccomp required,indicating
R3081 T4568 T4569 det a,segment
R3082 T4569 T4567 nsubjpass segment,required
R3083 T4570 T4571 amod single,stranded
R3084 T4571 T4569 amod stranded,segment
R3085 T4572 T4571 punct -,stranded
R3086 T4573 T4569 prep of,segment
R3087 T4574 T4575 advmod at,least
R3088 T4575 T4576 advmod least,40
R3089 T4576 T4577 nummod 40,nt
R3090 T4577 T4573 pobj nt,of
R3091 T4578 T4577 amod long,nt
R3092 T4579 T4567 auxpass is,required
R3093 T4580 T4567 prep for,required
R3094 T4581 T4582 amod efficient,actions
R3095 T4582 T4580 pobj actions,for
R3096 T4583 T4582 compound helicase,actions
R3097 T4584 T4582 prep on,actions
R3098 T4585 T4586 compound bubble,substrates
R3099 T4586 T4584 pobj substrates,on
R3100 T4587 T4588 punct (,2B
R3101 T4588 T4567 parataxis 2B,required
R3102 T4589 T4588 compound Figure,2B
R3103 T4590 T4588 punct ),2B
R3104 T4591 T4547 punct .,observed
R3105 T4593 T4594 advcl Taken,are
R3106 T4595 T4593 advmod together,Taken
R3107 T4596 T4594 punct ", ",are
R3108 T4597 T4598 det the,results
R3109 T4598 T4594 nsubj results,are
R3110 T4599 T4598 amod above,results
R3111 T4600 T4594 acomp consistent,are
R3112 T4601 T4600 prep with,consistent
R3113 T4602 T4603 det the,notion
R3114 T4603 T4601 pobj notion,with
R3115 T4604 T4605 mark that,unwinds
R3116 T4605 T4603 acl unwinds,notion
R3117 T4606 T4607 det the,complex
R3118 T4607 T4605 nsubj complex,unwinds
R3119 T4608 T4607 nmod Mcm4,complex
R3120 T4609 T4608 punct /,Mcm4
R3121 T4610 T4608 nummod 6,Mcm4
R3122 T4611 T4608 punct /,Mcm4
R3123 T4612 T4608 nummod 7,Mcm4
R3124 T4613 T4607 punct ", ",complex
R3125 T4614 T4607 acl loaded,complex
R3126 T4615 T4614 prep onto,loaded
R3127 T4616 T4615 pobj DNA,onto
R3128 T4617 T4614 prep as,loaded
R3129 T4618 T4619 det a,complex
R3130 T4619 T4617 pobj complex,as
R3131 T4620 T4621 amod double,hexameric
R3132 T4621 T4619 amod hexameric,complex
R3133 T4622 T4605 punct ", ",unwinds
R3134 T4623 T4605 advmod efficiently,unwinds
R3135 T4624 T4625 det the,region
R3136 T4625 T4605 dobj region,unwinds
R3137 T4626 T4625 compound duplex,region
R3138 T4627 T4625 prep of,region
R3139 T4628 T4629 det the,DNA
R3140 T4629 T4627 pobj DNA,of
R3141 T4630 T4629 compound substrate,DNA
R3142 T4631 T4594 punct .,are

craft-ca-core-ex-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T4006 0-12 _FRAGMENT denotes Formation of
T4007 39-48 GO:0065003 denotes complexes
T4008 13-29 _FRAGMENT denotes double hexameric
T4009 39-48 GO_EXT:double_hexameric_macromolecular_complex denotes complexes
T4010 30-34 PR_EXT:000010246 denotes Mcm4
T4011 30-38 GO:0097373 denotes Mcm4/6/7
T4012 61-69 GO_EXT:0004386 denotes helicase
T4013 130-134 PR_EXT:000010246 denotes Mcm4
T4014 130-138 GO:0097373 denotes Mcm4/6/7
T4015 151-175 GO_EXT:double_hexameric_macromolecular_complex denotes double hexameric complex
T4016 194-201 GO_EXT:hexameric_macromolecular_complex denotes hexamer
T4017 264-279 SO:0000984 denotes single-stranded
T4018 312-327 SO:0000984 denotes single-stranded
T4019 399-410 GO:0042555 denotes Mcm complex
T4020 451-455 PR_EXT:000010246 denotes Mcm4
T4021 451-459 GO:0097373 denotes Mcm4/6/7
T4022 475-499 GO_EXT:hexameric_macromolecular_complex denotes single hexameric complex
T4023 541-543 CHEBI_SO_EXT:nucleotide denotes nt
T4024 557-572 SO:0000984 denotes single-stranded
T4025 588-612 GO_EXT:double_hexameric_macromolecular_complex denotes double hexameric complex
T4026 626-629 CHEBI_SO_EXT:DNA denotes DNA
T4027 686-700 GO_EXT:double_hexameric_macromolecular_complex denotes double-hexamer
T4028 846-854 SO:0000984 denotes unpaired
T4029 889-896 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes binding
T4030 901-909 GO_EXT:0004386 denotes helicase
T4031 924-928 PR_EXT:000010246 denotes Mcm4
T4032 924-932 GO:0097373 denotes Mcm4/6/7
T4033 968-972 PR_EXT:000010246 denotes Mcm4
T4034 968-976 GO:0097373 denotes Mcm4/6/7
T4035 998-1010 CHEBI_EXT:radiolabel_process denotes radiolabeled
T4036 1064-1071 CHEBI:27575 denotes ATP-γ-S
T4037 1081-1088 GO:0032991 denotes complex
T4038 1081-1098 GO:0065003 denotes complex formation
T4039 1125-1133 CHEBI:60004 denotes mixtures
T4040 1152-1155 CHEBI_SO_EXT:DNA denotes DNA
T4041 1156-1163 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes binding
T4042 1262-1265 CHEBI_EXT:ATP denotes ATP
T4043 1277-1280 CHEBI_SO_EXT:DNA denotes DNA
T4044 1277-1289 GO_EXT:0003678 denotes DNA helicase
T4045 1349-1358 GO:0032991 denotes complexes
T4046 1364-1368 PR_EXT:000010246 denotes Mcm4
T4047 1364-1372 GO:0097373 denotes Mcm4/6/7
T4048 1464-1471 GO_EXT:hexameric_macromolecular_complex denotes hexamer
T4049 1475-1479 PR_EXT:000010246 denotes Mcm4
T4050 1475-1483 GO:0097373 denotes Mcm4/6/7
T4051 1518-1527 GO:0032991 denotes complexes
T4052 1541-1555 GO_EXT:double_hexameric_macromolecular_complex denotes double hexamer
T4053 1612-1619 GO_EXT:hexameric_macromolecular_complex denotes hexamer
T4054 1656-1680 GO_EXT:double_hexameric_macromolecular_complex denotes double hexamer complexes
T4055 1743-1767 GO_EXT:hexameric_macromolecular_complex denotes single hexamer complexes
T4056 1809-1824 SO:0000984 denotes single-stranded
T4057 1848-1850 CHEBI_SO_EXT:nucleotide denotes nt
T4058 1897-1913 GO_EXT:double_hexameric_macromolecular_complex denotes double-hexameric
T4059 1914-1917 GO:0042555 denotes Mcm
T4060 1918-1926 GO_EXT:0004386 denotes helicase
T4061 1953-1956 CHEBI_SO_EXT:DNA denotes DNA
T4062 1953-1965 GO_EXT:0003678 denotes DNA helicase
T4063 1974-1978 PR_EXT:000010246 denotes Mcm4
T4064 1974-1990 GO:0097373 denotes Mcm4/6/7 complex
T4065 2004-2012 GO_EXT:0004386 denotes helicase
T4066 2130-2145 SO:0000984 denotes single-stranded
T4067 2173-2175 CHEBI_SO_EXT:nucleotide denotes nt
T4068 2277-2292 SO:0000984 denotes single-stranded
T4069 2316-2318 CHEBI_SO_EXT:nucleotide denotes nt
T4070 2350-2358 GO_EXT:0004386 denotes helicase
T4071 2475-2479 PR_EXT:000010246 denotes Mcm4
T4072 2475-2491 GO:0097373 denotes Mcm4/6/7 complex
T4073 2505-2508 CHEBI_SO_EXT:DNA denotes DNA
T4074 2514-2538 GO_EXT:double_hexameric_macromolecular_complex denotes double hexameric complex
T4075 2564-2570 SO:0000985 denotes duplex
T4076 2595-2598 CHEBI_SO_EXT:DNA denotes DNA
R2615 T4007 T4006 _lexicallyChainedTo complexes,Formation of
R2616 T4009 T4008 _lexicallyChainedTo complexes,double hexameric

craft-ca-core-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T3970 0-12 _FRAGMENT denotes Formation of
T3971 39-48 GO:0065003 denotes complexes
T3972 30-34 PR:000010246 denotes Mcm4
T3973 30-38 GO:0097373 denotes Mcm4/6/7
T3974 130-134 PR:000010246 denotes Mcm4
T3975 130-138 GO:0097373 denotes Mcm4/6/7
T3976 264-279 SO:0000984 denotes single-stranded
T3977 312-327 SO:0000984 denotes single-stranded
T3978 399-410 GO:0042555 denotes Mcm complex
T3979 451-455 PR:000010246 denotes Mcm4
T3980 451-459 GO:0097373 denotes Mcm4/6/7
T3981 557-572 SO:0000984 denotes single-stranded
T3982 846-854 SO:0000984 denotes unpaired
T3983 924-928 PR:000010246 denotes Mcm4
T3984 924-932 GO:0097373 denotes Mcm4/6/7
T3985 968-972 PR:000010246 denotes Mcm4
T3986 968-976 GO:0097373 denotes Mcm4/6/7
T3987 1064-1071 CHEBI:27575 denotes ATP-γ-S
T3988 1081-1088 GO:0032991 denotes complex
T3989 1081-1098 GO:0065003 denotes complex formation
T3990 1125-1133 CHEBI:60004 denotes mixtures
T3991 1349-1358 GO:0032991 denotes complexes
T3992 1364-1368 PR:000010246 denotes Mcm4
T3993 1364-1372 GO:0097373 denotes Mcm4/6/7
T3994 1475-1479 PR:000010246 denotes Mcm4
T3995 1475-1483 GO:0097373 denotes Mcm4/6/7
T3996 1518-1527 GO:0032991 denotes complexes
T3997 1809-1824 SO:0000984 denotes single-stranded
T3998 1914-1917 GO:0042555 denotes Mcm
T3999 1974-1978 PR:000010246 denotes Mcm4
T4000 1974-1990 GO:0097373 denotes Mcm4/6/7 complex
T4001 2130-2145 SO:0000984 denotes single-stranded
T4002 2277-2292 SO:0000984 denotes single-stranded
T4003 2475-2479 PR:000010246 denotes Mcm4
T4004 2475-2491 GO:0097373 denotes Mcm4/6/7 complex
T4005 2564-2570 SO:0000985 denotes duplex
R2614 T3971 T3970 _lexicallyChainedTo complexes,Formation of

2_test

Id Subject Object Predicate Lexical cue
15917436-14609960-76775510 631-633 14609960 denotes 13
15917436-14609960-76775511 1633-1635 14609960 denotes 13
15917436-9765408-76775512 1636-1638 9765408 denotes 21