Id |
Subject |
Object |
Predicate |
Lexical cue |
T3477 |
293-302 |
VBD |
denotes |
conducted |
T3428 |
0-4 |
NN |
denotes |
Mcm4 |
T3429 |
9-16 |
NN |
denotes |
complex |
T3430 |
4-5 |
HYPH |
denotes |
/ |
T3431 |
5-6 |
CD |
denotes |
6 |
T3432 |
6-7 |
HYPH |
denotes |
/ |
T3433 |
7-8 |
CD |
denotes |
7 |
T3434 |
27-36 |
VBZ |
denotes |
interacts |
T3435 |
17-26 |
RB |
denotes |
primarily |
T3436 |
37-41 |
IN |
denotes |
with |
T3437 |
42-45 |
DT |
denotes |
the |
T3438 |
66-73 |
NN |
denotes |
segment |
T3439 |
46-52 |
JJ |
denotes |
single |
T3440 |
53-61 |
VBN |
denotes |
stranded |
T3441 |
52-53 |
HYPH |
denotes |
- |
T3442 |
62-65 |
NN |
denotes |
DNA |
T3443 |
73-221 |
sentence |
denotes |
Mcm4/6/7 binds to ‘bubble’ DNA substrates and unwinds the duplex segments when the central single-stranded segment contains thymine stretches (13). |
T3444 |
74-78 |
NN |
denotes |
Mcm4 |
T3445 |
83-88 |
VBZ |
denotes |
binds |
T3446 |
78-79 |
HYPH |
denotes |
/ |
T3447 |
79-80 |
CD |
denotes |
6 |
T3448 |
80-81 |
HYPH |
denotes |
/ |
T3449 |
81-82 |
CD |
denotes |
7 |
T3450 |
89-91 |
IN |
denotes |
to |
T3451 |
92-93 |
`` |
denotes |
‘ |
T3452 |
105-115 |
NNS |
denotes |
substrates |
T3453 |
93-99 |
NN |
denotes |
bubble |
T3454 |
99-100 |
'' |
denotes |
’ |
T3455 |
101-104 |
NN |
denotes |
DNA |
T3456 |
116-119 |
CC |
denotes |
and |
T3457 |
120-127 |
VBZ |
denotes |
unwinds |
T3458 |
128-131 |
DT |
denotes |
the |
T3459 |
139-147 |
NNS |
denotes |
segments |
T3460 |
132-138 |
NN |
denotes |
duplex |
T3461 |
148-152 |
WRB |
denotes |
when |
T3462 |
189-197 |
VBZ |
denotes |
contains |
T3463 |
153-156 |
DT |
denotes |
the |
T3464 |
181-188 |
NN |
denotes |
segment |
T3465 |
157-164 |
JJ |
denotes |
central |
T3466 |
165-171 |
JJ |
denotes |
single |
T3467 |
172-180 |
VBN |
denotes |
stranded |
T3468 |
171-172 |
HYPH |
denotes |
- |
T3469 |
198-205 |
NN |
denotes |
thymine |
T3470 |
206-215 |
NNS |
denotes |
stretches |
T3471 |
216-217 |
-LRB- |
denotes |
( |
T3472 |
217-219 |
CD |
denotes |
13 |
T3473 |
219-220 |
-RRB- |
denotes |
) |
T3474 |
220-221 |
. |
denotes |
. |
T3475 |
221-480 |
sentence |
denotes |
In order to examine the mode of binding of Mcm4/6/7 on bubble DNAs, we conducted nuclease protection assays using labeled bubble substrate DNAs and Mcm4/6/7 in the presence of ATP-γ-S which permits binding to substrate DNA but does not mobilize the helicase. |
T3476 |
222-224 |
IN |
denotes |
In |
T3478 |
225-230 |
NN |
denotes |
order |
T3479 |
231-233 |
TO |
denotes |
to |
T3480 |
234-241 |
VB |
denotes |
examine |
T3481 |
242-245 |
DT |
denotes |
the |
T3482 |
246-250 |
NN |
denotes |
mode |
T3483 |
251-253 |
IN |
denotes |
of |
T3484 |
254-261 |
NN |
denotes |
binding |
T3485 |
262-264 |
IN |
denotes |
of |
T3486 |
265-269 |
NN |
denotes |
Mcm4 |
T3487 |
269-270 |
HYPH |
denotes |
/ |
T3488 |
270-271 |
CD |
denotes |
6 |
T3489 |
271-272 |
HYPH |
denotes |
/ |
T3490 |
272-273 |
CD |
denotes |
7 |
T3491 |
274-276 |
IN |
denotes |
on |
T3492 |
277-283 |
NN |
denotes |
bubble |
T3493 |
284-288 |
NNS |
denotes |
DNAs |
T3494 |
288-290 |
, |
denotes |
, |
T3495 |
290-292 |
PRP |
denotes |
we |
T3496 |
303-311 |
NN |
denotes |
nuclease |
T3497 |
323-329 |
NNS |
denotes |
assays |
T3498 |
312-322 |
NN |
denotes |
protection |
T3499 |
330-335 |
VBG |
denotes |
using |
T3500 |
336-343 |
VBN |
denotes |
labeled |
T3501 |
361-365 |
NNS |
denotes |
DNAs |
T3502 |
344-350 |
NN |
denotes |
bubble |
T3503 |
351-360 |
NN |
denotes |
substrate |
T3504 |
366-369 |
CC |
denotes |
and |
T3505 |
370-374 |
NN |
denotes |
Mcm4 |
T3506 |
374-375 |
HYPH |
denotes |
/ |
T3507 |
375-376 |
CD |
denotes |
6 |
T3508 |
376-377 |
HYPH |
denotes |
/ |
T3509 |
377-378 |
CD |
denotes |
7 |
T3510 |
379-381 |
IN |
denotes |
in |
T3511 |
382-385 |
DT |
denotes |
the |
T3512 |
386-394 |
NN |
denotes |
presence |
T3513 |
395-397 |
IN |
denotes |
of |
T3514 |
398-401 |
NN |
denotes |
ATP |
T3515 |
404-405 |
NN |
denotes |
S |
T3516 |
401-402 |
HYPH |
denotes |
- |
T3517 |
402-403 |
SYM |
denotes |
γ |
T3518 |
403-404 |
HYPH |
denotes |
- |
T3519 |
406-411 |
WDT |
denotes |
which |
T3520 |
412-419 |
VBZ |
denotes |
permits |
T3521 |
420-427 |
NN |
denotes |
binding |
T3522 |
428-430 |
IN |
denotes |
to |
T3523 |
431-440 |
NN |
denotes |
substrate |
T3524 |
441-444 |
NN |
denotes |
DNA |
T3525 |
445-448 |
CC |
denotes |
but |
T3526 |
449-453 |
VBZ |
denotes |
does |
T3527 |
458-466 |
VB |
denotes |
mobilize |
T3528 |
454-457 |
RB |
denotes |
not |
T3529 |
467-470 |
DT |
denotes |
the |
T3530 |
471-479 |
NN |
denotes |
helicase |
T3531 |
479-480 |
. |
denotes |
. |
T3532 |
480-751 |
sentence |
denotes |
At an optimum concentration of DNase I, strong cleavages were detected on the 21 bp duplex regions at both ends of the substrates, whereas weaker cleavages were detected on the central single-stranded region, consistent with preference of DNase I to double-stranded DNA. |
T3533 |
481-483 |
IN |
denotes |
At |
T3534 |
543-551 |
VBN |
denotes |
detected |
T3535 |
484-486 |
DT |
denotes |
an |
T3536 |
495-508 |
NN |
denotes |
concentration |
T3537 |
487-494 |
JJ |
denotes |
optimum |
T3538 |
509-511 |
IN |
denotes |
of |
T3539 |
512-517 |
NN |
denotes |
DNase |
T3540 |
518-519 |
CD |
denotes |
I |
T3541 |
519-521 |
, |
denotes |
, |
T3542 |
521-527 |
JJ |
denotes |
strong |
T3543 |
528-537 |
NNS |
denotes |
cleavages |
T3544 |
538-542 |
VBD |
denotes |
were |
T3545 |
552-554 |
IN |
denotes |
on |
T3546 |
555-558 |
DT |
denotes |
the |
T3547 |
572-579 |
NNS |
denotes |
regions |
T3548 |
559-561 |
CD |
denotes |
21 |
T3549 |
562-564 |
NN |
denotes |
bp |
T3550 |
565-571 |
NN |
denotes |
duplex |
T3551 |
580-582 |
IN |
denotes |
at |
T3552 |
583-587 |
DT |
denotes |
both |
T3553 |
588-592 |
NNS |
denotes |
ends |
T3554 |
593-595 |
IN |
denotes |
of |
T3555 |
596-599 |
DT |
denotes |
the |
T3556 |
600-610 |
NNS |
denotes |
substrates |
T3557 |
610-612 |
, |
denotes |
, |
T3558 |
612-619 |
IN |
denotes |
whereas |
T3559 |
642-650 |
VBN |
denotes |
detected |
T3560 |
620-626 |
JJR |
denotes |
weaker |
T3561 |
627-636 |
NNS |
denotes |
cleavages |
T3562 |
637-641 |
VBD |
denotes |
were |
T3563 |
651-653 |
IN |
denotes |
on |
T3564 |
654-657 |
DT |
denotes |
the |
T3565 |
682-688 |
NN |
denotes |
region |
T3566 |
658-665 |
JJ |
denotes |
central |
T3567 |
666-672 |
JJ |
denotes |
single |
T3568 |
673-681 |
VBN |
denotes |
stranded |
T3569 |
672-673 |
HYPH |
denotes |
- |
T3570 |
688-690 |
, |
denotes |
, |
T3571 |
690-700 |
JJ |
denotes |
consistent |
T3572 |
701-705 |
IN |
denotes |
with |
T3573 |
706-716 |
NN |
denotes |
preference |
T3574 |
717-719 |
IN |
denotes |
of |
T3575 |
720-725 |
NN |
denotes |
DNase |
T3576 |
726-727 |
CD |
denotes |
I |
T3577 |
728-730 |
IN |
denotes |
to |
T3578 |
731-737 |
JJ |
denotes |
double |
T3579 |
738-746 |
VBN |
denotes |
stranded |
T3580 |
737-738 |
HYPH |
denotes |
- |
T3581 |
747-750 |
NN |
denotes |
DNA |
T3582 |
750-751 |
. |
denotes |
. |
T3583 |
751-1009 |
sentence |
denotes |
With the Bub66/T-rich substrate, strong protection was detected on the entire single-stranded regions of both top and bottom strands with increasing concentration of Mcm4/6/7, consistent with high affinity of Mcm4/6/7 to T-rich bubble sequences (Figure 1A). |
T3584 |
752-756 |
IN |
denotes |
With |
T3585 |
807-815 |
VBN |
denotes |
detected |
T3586 |
757-760 |
DT |
denotes |
the |
T3587 |
774-783 |
NN |
denotes |
substrate |
T3588 |
761-766 |
NN |
denotes |
Bub66 |
T3589 |
766-767 |
HYPH |
denotes |
/ |
T3590 |
767-768 |
NN |
denotes |
T |
T3591 |
768-769 |
HYPH |
denotes |
- |
T3592 |
769-773 |
JJ |
denotes |
rich |
T3593 |
783-785 |
, |
denotes |
, |
T3594 |
785-791 |
JJ |
denotes |
strong |
T3595 |
792-802 |
NN |
denotes |
protection |
T3596 |
803-806 |
VBD |
denotes |
was |
T3597 |
816-818 |
IN |
denotes |
on |
T3598 |
819-822 |
DT |
denotes |
the |
T3599 |
846-853 |
NNS |
denotes |
regions |
T3600 |
823-829 |
JJ |
denotes |
entire |
T3601 |
830-836 |
JJ |
denotes |
single |
T3602 |
837-845 |
VBN |
denotes |
stranded |
T3603 |
836-837 |
HYPH |
denotes |
- |
T3604 |
854-856 |
IN |
denotes |
of |
T3605 |
857-861 |
CC |
denotes |
both |
T3606 |
862-865 |
JJ |
denotes |
top |
T3607 |
877-884 |
NNS |
denotes |
strands |
T3608 |
866-869 |
CC |
denotes |
and |
T3609 |
870-876 |
JJ |
denotes |
bottom |
T3610 |
885-889 |
IN |
denotes |
with |
T3611 |
890-900 |
VBG |
denotes |
increasing |
T3612 |
901-914 |
NN |
denotes |
concentration |
T3613 |
915-917 |
IN |
denotes |
of |
T3614 |
918-922 |
NN |
denotes |
Mcm4 |
T3615 |
922-923 |
HYPH |
denotes |
/ |
T3616 |
923-924 |
CD |
denotes |
6 |
T3617 |
924-925 |
HYPH |
denotes |
/ |
T3618 |
925-926 |
CD |
denotes |
7 |
T3619 |
926-928 |
, |
denotes |
, |
T3620 |
928-938 |
JJ |
denotes |
consistent |
T3621 |
939-943 |
IN |
denotes |
with |
T3622 |
944-948 |
JJ |
denotes |
high |
T3623 |
949-957 |
NN |
denotes |
affinity |
T3624 |
958-960 |
IN |
denotes |
of |
T3625 |
961-965 |
NN |
denotes |
Mcm4 |
T3626 |
965-966 |
HYPH |
denotes |
/ |
T3627 |
966-967 |
CD |
denotes |
6 |
T3628 |
967-968 |
HYPH |
denotes |
/ |
T3629 |
968-969 |
CD |
denotes |
7 |
T3630 |
970-972 |
IN |
denotes |
to |
T3631 |
973-974 |
NN |
denotes |
T |
T3632 |
987-996 |
NNS |
denotes |
sequences |
T3633 |
974-975 |
HYPH |
denotes |
- |
T3634 |
975-979 |
JJ |
denotes |
rich |
T3635 |
980-986 |
NN |
denotes |
bubble |
T3636 |
997-998 |
-LRB- |
denotes |
( |
T3637 |
1005-1007 |
NN |
denotes |
1A |
T3638 |
998-1004 |
NN |
denotes |
Figure |
T3639 |
1007-1008 |
-RRB- |
denotes |
) |
T3640 |
1008-1009 |
. |
denotes |
. |
T3641 |
1009-1171 |
sentence |
denotes |
Similar, but less significant protection was detected on the single-stranded DNA segment with P1 nucleases (Figure 1A), which is specific to single-stranded DNA. |
T3642 |
1010-1017 |
JJ |
denotes |
Similar |
T3643 |
1040-1050 |
NN |
denotes |
protection |
T3644 |
1017-1019 |
, |
denotes |
, |
T3645 |
1019-1022 |
CC |
denotes |
but |
T3646 |
1023-1027 |
RBR |
denotes |
less |
T3647 |
1028-1039 |
JJ |
denotes |
significant |
T3648 |
1055-1063 |
VBN |
denotes |
detected |
T3649 |
1051-1054 |
VBD |
denotes |
was |
T3650 |
1064-1066 |
IN |
denotes |
on |
T3651 |
1067-1070 |
DT |
denotes |
the |
T3652 |
1091-1098 |
NN |
denotes |
segment |
T3653 |
1071-1077 |
JJ |
denotes |
single |
T3654 |
1078-1086 |
VBN |
denotes |
stranded |
T3655 |
1077-1078 |
HYPH |
denotes |
- |
T3656 |
1087-1090 |
NN |
denotes |
DNA |
T3657 |
1099-1103 |
IN |
denotes |
with |
T3658 |
1104-1106 |
NN |
denotes |
P1 |
T3659 |
1107-1116 |
NNS |
denotes |
nucleases |
T3660 |
1117-1118 |
-LRB- |
denotes |
( |
T3661 |
1125-1127 |
NN |
denotes |
1A |
T3662 |
1118-1124 |
NN |
denotes |
Figure |
T3663 |
1127-1128 |
-RRB- |
denotes |
) |
T3664 |
1128-1130 |
, |
denotes |
, |
T3665 |
1130-1135 |
WDT |
denotes |
which |
T3666 |
1136-1138 |
VBZ |
denotes |
is |
T3667 |
1139-1147 |
JJ |
denotes |
specific |
T3668 |
1148-1150 |
IN |
denotes |
to |
T3669 |
1151-1157 |
JJ |
denotes |
single |
T3670 |
1158-1166 |
VBN |
denotes |
stranded |
T3671 |
1157-1158 |
HYPH |
denotes |
- |
T3672 |
1167-1170 |
NN |
denotes |
DNA |
T3673 |
1170-1171 |
. |
denotes |
. |
T3674 |
1171-1302 |
sentence |
denotes |
The results indicate that the Mcm complex is loaded onto bubble DNA through binding to both strands of the single-stranded region. |
T3675 |
1172-1175 |
DT |
denotes |
The |
T3676 |
1176-1183 |
NNS |
denotes |
results |
T3677 |
1184-1192 |
VBP |
denotes |
indicate |
T3678 |
1193-1197 |
IN |
denotes |
that |
T3679 |
1217-1223 |
VBN |
denotes |
loaded |
T3680 |
1198-1201 |
DT |
denotes |
the |
T3681 |
1206-1213 |
NN |
denotes |
complex |
T3682 |
1202-1205 |
NN |
denotes |
Mcm |
T3683 |
1214-1216 |
VBZ |
denotes |
is |
T3684 |
1224-1228 |
IN |
denotes |
onto |
T3685 |
1229-1235 |
NN |
denotes |
bubble |
T3686 |
1236-1239 |
NN |
denotes |
DNA |
T3687 |
1240-1247 |
IN |
denotes |
through |
T3688 |
1248-1255 |
NN |
denotes |
binding |
T3689 |
1256-1258 |
IN |
denotes |
to |
T3690 |
1259-1263 |
DT |
denotes |
both |
T3691 |
1264-1271 |
NNS |
denotes |
strands |
T3692 |
1272-1274 |
IN |
denotes |
of |
T3693 |
1275-1278 |
DT |
denotes |
the |
T3694 |
1295-1301 |
NN |
denotes |
region |
T3695 |
1279-1285 |
JJ |
denotes |
single |
T3696 |
1286-1294 |
VBN |
denotes |
stranded |
T3697 |
1285-1286 |
HYPH |
denotes |
- |
T3698 |
1301-1302 |
. |
denotes |
. |
T3699 |
1302-1576 |
sentence |
denotes |
Careful examination of Mcm4/6/7 footprints on a bubble indicated strong protection on the 5′-half of the top strand and moderate protection on the remaining 3′-half, as well as on several base-pair duplex segment adjacent to the branch point (Figure 1A and data not shown). |
T3700 |
1303-1310 |
JJ |
denotes |
Careful |
T3701 |
1311-1322 |
NN |
denotes |
examination |
T3702 |
1358-1367 |
VBD |
denotes |
indicated |
T3703 |
1323-1325 |
IN |
denotes |
of |
T3704 |
1326-1330 |
NN |
denotes |
Mcm4 |
T3705 |
1335-1345 |
NNS |
denotes |
footprints |
T3706 |
1330-1331 |
HYPH |
denotes |
/ |
T3707 |
1331-1332 |
CD |
denotes |
6 |
T3708 |
1332-1333 |
HYPH |
denotes |
/ |
T3709 |
1333-1334 |
CD |
denotes |
7 |
T3710 |
1346-1348 |
IN |
denotes |
on |
T3711 |
1349-1350 |
DT |
denotes |
a |
T3712 |
1351-1357 |
NN |
denotes |
bubble |
T3713 |
1368-1374 |
JJ |
denotes |
strong |
T3714 |
1375-1385 |
NN |
denotes |
protection |
T3715 |
1386-1388 |
IN |
denotes |
on |
T3716 |
1389-1392 |
DT |
denotes |
the |
T3717 |
1396-1400 |
NN |
denotes |
half |
T3718 |
1393-1394 |
CD |
denotes |
5 |
T3719 |
1394-1395 |
SYM |
denotes |
′ |
T3720 |
1395-1396 |
HYPH |
denotes |
- |
T3721 |
1401-1403 |
IN |
denotes |
of |
T3722 |
1404-1407 |
DT |
denotes |
the |
T3723 |
1412-1418 |
NN |
denotes |
strand |
T3724 |
1408-1411 |
JJ |
denotes |
top |
T3725 |
1419-1422 |
CC |
denotes |
and |
T3726 |
1423-1431 |
JJ |
denotes |
moderate |
T3727 |
1432-1442 |
NN |
denotes |
protection |
T3728 |
1443-1445 |
IN |
denotes |
on |
T3729 |
1446-1449 |
DT |
denotes |
the |
T3730 |
1463-1467 |
NN |
denotes |
half |
T3731 |
1450-1459 |
VBG |
denotes |
remaining |
T3732 |
1460-1461 |
CD |
denotes |
3 |
T3733 |
1461-1462 |
SYM |
denotes |
′ |
T3734 |
1462-1463 |
HYPH |
denotes |
- |
T3735 |
1467-1469 |
, |
denotes |
, |
T3736 |
1469-1471 |
RB |
denotes |
as |
T3737 |
1477-1479 |
IN |
denotes |
as |
T3738 |
1472-1476 |
RB |
denotes |
well |
T3739 |
1480-1482 |
IN |
denotes |
on |
T3740 |
1483-1490 |
JJ |
denotes |
several |
T3741 |
1508-1515 |
NN |
denotes |
segment |
T3742 |
1491-1495 |
NN |
denotes |
base |
T3743 |
1496-1500 |
NN |
denotes |
pair |
T3744 |
1495-1496 |
HYPH |
denotes |
- |
T3745 |
1501-1507 |
NN |
denotes |
duplex |
T3746 |
1516-1524 |
JJ |
denotes |
adjacent |
T3747 |
1525-1527 |
IN |
denotes |
to |
T3748 |
1528-1531 |
DT |
denotes |
the |
T3749 |
1539-1544 |
NN |
denotes |
point |
T3750 |
1532-1538 |
NN |
denotes |
branch |
T3751 |
1545-1546 |
-LRB- |
denotes |
( |
T3752 |
1553-1555 |
NN |
denotes |
1A |
T3753 |
1546-1552 |
NN |
denotes |
Figure |
T3754 |
1556-1559 |
CC |
denotes |
and |
T3755 |
1560-1564 |
NNS |
denotes |
data |
T3756 |
1565-1568 |
RB |
denotes |
not |
T3757 |
1569-1574 |
VBN |
denotes |
shown |
T3758 |
1574-1575 |
-RRB- |
denotes |
) |
T3759 |
1575-1576 |
. |
denotes |
. |
T3760 |
1576-1648 |
sentence |
denotes |
A similar pattern of protection was observed also on the bottom strand. |
T3761 |
1577-1578 |
DT |
denotes |
A |
T3762 |
1587-1594 |
NN |
denotes |
pattern |
T3763 |
1579-1586 |
JJ |
denotes |
similar |
T3764 |
1613-1621 |
VBN |
denotes |
observed |
T3765 |
1595-1597 |
IN |
denotes |
of |
T3766 |
1598-1608 |
NN |
denotes |
protection |
T3767 |
1609-1612 |
VBD |
denotes |
was |
T3768 |
1622-1626 |
RB |
denotes |
also |
T3769 |
1627-1629 |
IN |
denotes |
on |
T3770 |
1630-1633 |
DT |
denotes |
the |
T3771 |
1641-1647 |
NN |
denotes |
strand |
T3772 |
1634-1640 |
JJ |
denotes |
bottom |
T3773 |
1647-1648 |
. |
denotes |
. |
T3774 |
1648-1841 |
sentence |
denotes |
This suggests that Mcm4/6/7 may bind to a replication bubble substrate with a 2-fold symmetry as a double hexamer (Figure 1A, see the lower scheme), in a manner similar to SV40 T-antigen (21). |
T3775 |
1649-1653 |
DT |
denotes |
This |
T3776 |
1654-1662 |
VBZ |
denotes |
suggests |
T3777 |
1663-1667 |
IN |
denotes |
that |
T3778 |
1681-1685 |
VB |
denotes |
bind |
T3779 |
1668-1672 |
NN |
denotes |
Mcm4 |
T3780 |
1672-1673 |
HYPH |
denotes |
/ |
T3781 |
1673-1674 |
CD |
denotes |
6 |
T3782 |
1674-1675 |
HYPH |
denotes |
/ |
T3783 |
1675-1676 |
CD |
denotes |
7 |
T3784 |
1677-1680 |
MD |
denotes |
may |
T3785 |
1686-1688 |
IN |
denotes |
to |
T3786 |
1689-1690 |
DT |
denotes |
a |
T3787 |
1710-1719 |
NN |
denotes |
substrate |
T3788 |
1691-1702 |
NN |
denotes |
replication |
T3789 |
1703-1709 |
NN |
denotes |
bubble |
T3790 |
1720-1724 |
IN |
denotes |
with |
T3791 |
1725-1726 |
DT |
denotes |
a |
T3792 |
1734-1742 |
NN |
denotes |
symmetry |
T3793 |
1727-1733 |
CD |
denotes |
2-fold |
T3794 |
1743-1745 |
IN |
denotes |
as |
T3795 |
1746-1747 |
DT |
denotes |
a |
T3796 |
1755-1762 |
NN |
denotes |
hexamer |
T3797 |
1748-1754 |
JJ |
denotes |
double |
T3798 |
1763-1764 |
-LRB- |
denotes |
( |
T3799 |
1771-1773 |
NN |
denotes |
1A |
T3800 |
1764-1770 |
NN |
denotes |
Figure |
T3801 |
1773-1775 |
, |
denotes |
, |
T3802 |
1775-1778 |
VBP |
denotes |
see |
T3803 |
1779-1782 |
DT |
denotes |
the |
T3804 |
1789-1795 |
NN |
denotes |
scheme |
T3805 |
1783-1788 |
JJ |
denotes |
lower |
T3806 |
1795-1796 |
-RRB- |
denotes |
) |
T3807 |
1796-1798 |
, |
denotes |
, |
T3808 |
1798-1800 |
IN |
denotes |
in |
T3809 |
1801-1802 |
DT |
denotes |
a |
T3810 |
1803-1809 |
NN |
denotes |
manner |
T3811 |
1810-1817 |
JJ |
denotes |
similar |
T3812 |
1818-1820 |
IN |
denotes |
to |
T3813 |
1821-1825 |
NN |
denotes |
SV40 |
T3814 |
1828-1835 |
NN |
denotes |
antigen |
T3815 |
1826-1827 |
NN |
denotes |
T |
T3816 |
1827-1828 |
HYPH |
denotes |
- |
T3817 |
1836-1837 |
-LRB- |
denotes |
( |
T3818 |
1837-1839 |
CD |
denotes |
21 |
T3819 |
1839-1840 |
-RRB- |
denotes |
) |
T3820 |
1840-1841 |
. |
denotes |
. |
T3821 |
1841-1932 |
sentence |
denotes |
Nuclease protection assays were conducted also on a T-tailed Y-fork structure (Figure 1B). |
T3822 |
1842-1850 |
NN |
denotes |
Nuclease |
T3823 |
1851-1861 |
NN |
denotes |
protection |
T3824 |
1862-1868 |
NNS |
denotes |
assays |
T3825 |
1874-1883 |
VBN |
denotes |
conducted |
T3826 |
1869-1873 |
VBD |
denotes |
were |
T3827 |
1884-1888 |
RB |
denotes |
also |
T3828 |
1889-1891 |
IN |
denotes |
on |
T3829 |
1892-1893 |
DT |
denotes |
a |
T3830 |
1910-1919 |
NN |
denotes |
structure |
T3831 |
1894-1895 |
NN |
denotes |
T |
T3832 |
1896-1902 |
VBN |
denotes |
tailed |
T3833 |
1895-1896 |
HYPH |
denotes |
- |
T3834 |
1903-1904 |
NN |
denotes |
Y |
T3835 |
1905-1909 |
NN |
denotes |
fork |
T3836 |
1904-1905 |
HYPH |
denotes |
- |
T3837 |
1920-1921 |
-LRB- |
denotes |
( |
T3838 |
1928-1930 |
NN |
denotes |
1B |
T3839 |
1921-1927 |
NN |
denotes |
Figure |
T3840 |
1930-1931 |
-RRB- |
denotes |
) |
T3841 |
1931-1932 |
. |
denotes |
. |
T3842 |
1932-2104 |
sentence |
denotes |
Not only the single-stranded tail region but also the 7 bp duplex DNA adjacent to the duplex-to-single-strand junction were protected by Mcm binding from DNaseI digestion. |
T3843 |
1933-1936 |
RB |
denotes |
Not |
T3844 |
1967-1973 |
NN |
denotes |
region |
T3845 |
1937-1941 |
RB |
denotes |
only |
T3846 |
1942-1945 |
DT |
denotes |
the |
T3847 |
1946-1952 |
JJ |
denotes |
single |
T3848 |
1953-1961 |
VBN |
denotes |
stranded |
T3849 |
1952-1953 |
HYPH |
denotes |
- |
T3850 |
1962-1966 |
NN |
denotes |
tail |
T3851 |
2057-2066 |
VBN |
denotes |
protected |
T3852 |
1974-1977 |
CC |
denotes |
but |
T3853 |
1978-1982 |
RB |
denotes |
also |
T3854 |
1983-1986 |
DT |
denotes |
the |
T3855 |
1999-2002 |
NN |
denotes |
DNA |
T3856 |
1987-1988 |
CD |
denotes |
7 |
T3857 |
1989-1991 |
NN |
denotes |
bp |
T3858 |
1992-1998 |
NN |
denotes |
duplex |
T3859 |
2003-2011 |
JJ |
denotes |
adjacent |
T3860 |
2012-2014 |
IN |
denotes |
to |
T3861 |
2015-2018 |
DT |
denotes |
the |
T3862 |
2043-2051 |
NN |
denotes |
junction |
T3863 |
2019-2025 |
NN |
denotes |
duplex |
T3864 |
2025-2026 |
HYPH |
denotes |
- |
T3865 |
2026-2028 |
IN |
denotes |
to |
T3866 |
2028-2029 |
HYPH |
denotes |
- |
T3867 |
2029-2035 |
JJ |
denotes |
single |
T3868 |
2036-2042 |
NN |
denotes |
strand |
T3869 |
2035-2036 |
HYPH |
denotes |
- |
T3870 |
2052-2056 |
VBD |
denotes |
were |
T3871 |
2067-2069 |
IN |
denotes |
by |
T3872 |
2070-2073 |
NN |
denotes |
Mcm |
T3873 |
2074-2081 |
NN |
denotes |
binding |
T3874 |
2082-2086 |
IN |
denotes |
from |
T3875 |
2087-2093 |
NN |
denotes |
DNaseI |
T3876 |
2094-2103 |
NN |
denotes |
digestion |
T3877 |
2103-2104 |
. |
denotes |
. |
T3878 |
2104-2303 |
sentence |
denotes |
These results indicate that Mcm4/6/7 protein primarily interacts with single-stranded DNA region and that the interaction also spans the duplex segments near the branchpoint of the replication fork. |
T3879 |
2105-2110 |
DT |
denotes |
These |
T3880 |
2111-2118 |
NNS |
denotes |
results |
T3881 |
2119-2127 |
VBP |
denotes |
indicate |
T3882 |
2128-2132 |
IN |
denotes |
that |
T3883 |
2160-2169 |
VBZ |
denotes |
interacts |
T3884 |
2133-2137 |
NN |
denotes |
Mcm4 |
T3885 |
2142-2149 |
NN |
denotes |
protein |
T3886 |
2137-2138 |
HYPH |
denotes |
/ |
T3887 |
2138-2139 |
CD |
denotes |
6 |
T3888 |
2139-2140 |
HYPH |
denotes |
/ |
T3889 |
2140-2141 |
CD |
denotes |
7 |
T3890 |
2150-2159 |
RB |
denotes |
primarily |
T3891 |
2170-2174 |
IN |
denotes |
with |
T3892 |
2175-2181 |
JJ |
denotes |
single |
T3893 |
2182-2190 |
VBN |
denotes |
stranded |
T3894 |
2181-2182 |
HYPH |
denotes |
- |
T3895 |
2195-2201 |
NN |
denotes |
region |
T3896 |
2191-2194 |
NN |
denotes |
DNA |
T3897 |
2202-2205 |
CC |
denotes |
and |
T3898 |
2206-2210 |
IN |
denotes |
that |
T3899 |
2232-2237 |
VBZ |
denotes |
spans |
T3900 |
2211-2214 |
DT |
denotes |
the |
T3901 |
2215-2226 |
NN |
denotes |
interaction |
T3902 |
2227-2231 |
RB |
denotes |
also |
T3903 |
2238-2241 |
DT |
denotes |
the |
T3904 |
2249-2257 |
NNS |
denotes |
segments |
T3905 |
2242-2248 |
NN |
denotes |
duplex |
T3906 |
2258-2262 |
IN |
denotes |
near |
T3907 |
2263-2266 |
DT |
denotes |
the |
T3908 |
2267-2278 |
NN |
denotes |
branchpoint |
T3909 |
2279-2281 |
IN |
denotes |
of |
T3910 |
2282-2285 |
DT |
denotes |
the |
T3911 |
2298-2302 |
NN |
denotes |
fork |
T3912 |
2286-2297 |
NN |
denotes |
replication |
T3913 |
2302-2303 |
. |
denotes |
. |
R2153 |
T3428 |
T3429 |
nmod |
Mcm4,complex |
R2154 |
T3429 |
T3434 |
nsubj |
complex,interacts |
R2155 |
T3430 |
T3428 |
punct |
/,Mcm4 |
R2156 |
T3431 |
T3428 |
nummod |
6,Mcm4 |
R2157 |
T3432 |
T3428 |
punct |
/,Mcm4 |
R2158 |
T3433 |
T3428 |
nummod |
7,Mcm4 |
R2159 |
T3435 |
T3434 |
advmod |
primarily,interacts |
R2160 |
T3436 |
T3434 |
prep |
with,interacts |
R2161 |
T3437 |
T3438 |
det |
the,segment |
R2162 |
T3438 |
T3436 |
pobj |
segment,with |
R2163 |
T3439 |
T3440 |
amod |
single,stranded |
R2164 |
T3440 |
T3438 |
amod |
stranded,segment |
R2165 |
T3441 |
T3440 |
punct |
-,stranded |
R2166 |
T3442 |
T3438 |
compound |
DNA,segment |
R2167 |
T3444 |
T3445 |
nsubj |
Mcm4,binds |
R2168 |
T3446 |
T3444 |
punct |
/,Mcm4 |
R2169 |
T3447 |
T3444 |
nummod |
6,Mcm4 |
R2170 |
T3448 |
T3444 |
punct |
/,Mcm4 |
R2171 |
T3449 |
T3444 |
nummod |
7,Mcm4 |
R2172 |
T3450 |
T3445 |
prep |
to,binds |
R2173 |
T3451 |
T3452 |
punct |
‘,substrates |
R2174 |
T3452 |
T3450 |
pobj |
substrates,to |
R2175 |
T3453 |
T3452 |
nmod |
bubble,substrates |
R2176 |
T3454 |
T3452 |
punct |
’,substrates |
R2177 |
T3455 |
T3452 |
compound |
DNA,substrates |
R2178 |
T3456 |
T3445 |
cc |
and,binds |
R2179 |
T3457 |
T3445 |
conj |
unwinds,binds |
R2180 |
T3458 |
T3459 |
det |
the,segments |
R2181 |
T3459 |
T3457 |
dobj |
segments,unwinds |
R2182 |
T3460 |
T3459 |
compound |
duplex,segments |
R2183 |
T3461 |
T3462 |
advmod |
when,contains |
R2184 |
T3462 |
T3457 |
advcl |
contains,unwinds |
R2185 |
T3463 |
T3464 |
det |
the,segment |
R2186 |
T3464 |
T3462 |
nsubj |
segment,contains |
R2187 |
T3465 |
T3464 |
amod |
central,segment |
R2188 |
T3466 |
T3467 |
amod |
single,stranded |
R2189 |
T3467 |
T3464 |
amod |
stranded,segment |
R2190 |
T3468 |
T3467 |
punct |
-,stranded |
R2191 |
T3469 |
T3470 |
compound |
thymine,stretches |
R2192 |
T3470 |
T3462 |
dobj |
stretches,contains |
R2193 |
T3471 |
T3472 |
punct |
(,13 |
R2194 |
T3472 |
T3462 |
parataxis |
13,contains |
R2195 |
T3473 |
T3472 |
punct |
),13 |
R2196 |
T3474 |
T3445 |
punct |
.,binds |
R2197 |
T3476 |
T3477 |
prep |
In,conducted |
R2198 |
T3478 |
T3476 |
pobj |
order,In |
R2199 |
T3479 |
T3480 |
aux |
to,examine |
R2200 |
T3480 |
T3478 |
acl |
examine,order |
R2201 |
T3481 |
T3482 |
det |
the,mode |
R2202 |
T3482 |
T3480 |
dobj |
mode,examine |
R2203 |
T3483 |
T3482 |
prep |
of,mode |
R2204 |
T3484 |
T3483 |
pobj |
binding,of |
R2205 |
T3485 |
T3484 |
prep |
of,binding |
R2206 |
T3486 |
T3485 |
pobj |
Mcm4,of |
R2207 |
T3487 |
T3486 |
punct |
/,Mcm4 |
R2208 |
T3488 |
T3486 |
nummod |
6,Mcm4 |
R2209 |
T3489 |
T3486 |
punct |
/,Mcm4 |
R2210 |
T3490 |
T3486 |
nummod |
7,Mcm4 |
R2211 |
T3491 |
T3484 |
prep |
on,binding |
R2212 |
T3492 |
T3493 |
compound |
bubble,DNAs |
R2213 |
T3493 |
T3491 |
pobj |
DNAs,on |
R2214 |
T3494 |
T3477 |
punct |
", ",conducted |
R2215 |
T3495 |
T3477 |
nsubj |
we,conducted |
R2216 |
T3496 |
T3497 |
compound |
nuclease,assays |
R2217 |
T3497 |
T3477 |
dobj |
assays,conducted |
R2218 |
T3498 |
T3497 |
compound |
protection,assays |
R2219 |
T3499 |
T3477 |
advcl |
using,conducted |
R2220 |
T3500 |
T3501 |
amod |
labeled,DNAs |
R2221 |
T3501 |
T3499 |
dobj |
DNAs,using |
R2222 |
T3502 |
T3503 |
compound |
bubble,substrate |
R2223 |
T3503 |
T3501 |
compound |
substrate,DNAs |
R2224 |
T3504 |
T3501 |
cc |
and,DNAs |
R2225 |
T3505 |
T3501 |
conj |
Mcm4,DNAs |
R2226 |
T3506 |
T3505 |
punct |
/,Mcm4 |
R2227 |
T3507 |
T3505 |
nummod |
6,Mcm4 |
R2228 |
T3508 |
T3505 |
punct |
/,Mcm4 |
R2229 |
T3509 |
T3505 |
nummod |
7,Mcm4 |
R2230 |
T3510 |
T3477 |
prep |
in,conducted |
R2231 |
T3511 |
T3512 |
det |
the,presence |
R2232 |
T3512 |
T3510 |
pobj |
presence,in |
R2233 |
T3513 |
T3512 |
prep |
of,presence |
R2234 |
T3514 |
T3515 |
nmod |
ATP,S |
R2235 |
T3515 |
T3513 |
pobj |
S,of |
R2236 |
T3516 |
T3515 |
punct |
-,S |
R2237 |
T3517 |
T3515 |
punct |
γ,S |
R2238 |
T3518 |
T3515 |
punct |
-,S |
R2239 |
T3519 |
T3520 |
dep |
which,permits |
R2240 |
T3520 |
T3515 |
relcl |
permits,S |
R2241 |
T3521 |
T3520 |
dobj |
binding,permits |
R2242 |
T3522 |
T3521 |
prep |
to,binding |
R2243 |
T3523 |
T3524 |
compound |
substrate,DNA |
R2244 |
T3524 |
T3522 |
pobj |
DNA,to |
R2245 |
T3525 |
T3520 |
cc |
but,permits |
R2246 |
T3526 |
T3527 |
aux |
does,mobilize |
R2247 |
T3527 |
T3520 |
conj |
mobilize,permits |
R2248 |
T3528 |
T3527 |
neg |
not,mobilize |
R2249 |
T3529 |
T3530 |
det |
the,helicase |
R2250 |
T3530 |
T3527 |
dobj |
helicase,mobilize |
R2251 |
T3531 |
T3477 |
punct |
.,conducted |
R2252 |
T3533 |
T3534 |
prep |
At,detected |
R2253 |
T3535 |
T3536 |
det |
an,concentration |
R2254 |
T3536 |
T3533 |
pobj |
concentration,At |
R2255 |
T3537 |
T3536 |
amod |
optimum,concentration |
R2256 |
T3538 |
T3536 |
prep |
of,concentration |
R2257 |
T3539 |
T3538 |
pobj |
DNase,of |
R2258 |
T3540 |
T3539 |
nummod |
I,DNase |
R2259 |
T3541 |
T3534 |
punct |
", ",detected |
R2260 |
T3542 |
T3543 |
amod |
strong,cleavages |
R2261 |
T3543 |
T3534 |
nsubjpass |
cleavages,detected |
R2262 |
T3544 |
T3534 |
auxpass |
were,detected |
R2263 |
T3545 |
T3534 |
prep |
on,detected |
R2264 |
T3546 |
T3547 |
det |
the,regions |
R2265 |
T3547 |
T3545 |
pobj |
regions,on |
R2266 |
T3548 |
T3549 |
nummod |
21,bp |
R2267 |
T3549 |
T3547 |
compound |
bp,regions |
R2268 |
T3550 |
T3547 |
compound |
duplex,regions |
R2269 |
T3551 |
T3534 |
prep |
at,detected |
R2270 |
T3552 |
T3553 |
det |
both,ends |
R2271 |
T3553 |
T3551 |
pobj |
ends,at |
R2272 |
T3554 |
T3553 |
prep |
of,ends |
R2273 |
T3555 |
T3556 |
det |
the,substrates |
R2274 |
T3556 |
T3554 |
pobj |
substrates,of |
R2275 |
T3557 |
T3534 |
punct |
", ",detected |
R2276 |
T3558 |
T3559 |
mark |
whereas,detected |
R2277 |
T3559 |
T3534 |
advcl |
detected,detected |
R2278 |
T3560 |
T3561 |
amod |
weaker,cleavages |
R2279 |
T3561 |
T3559 |
nsubjpass |
cleavages,detected |
R2280 |
T3562 |
T3559 |
auxpass |
were,detected |
R2281 |
T3563 |
T3559 |
prep |
on,detected |
R2282 |
T3564 |
T3565 |
det |
the,region |
R2283 |
T3565 |
T3563 |
pobj |
region,on |
R2284 |
T3566 |
T3565 |
amod |
central,region |
R2285 |
T3567 |
T3568 |
amod |
single,stranded |
R2286 |
T3568 |
T3565 |
amod |
stranded,region |
R2287 |
T3569 |
T3568 |
punct |
-,stranded |
R2288 |
T3570 |
T3559 |
punct |
", ",detected |
R2289 |
T3571 |
T3559 |
advcl |
consistent,detected |
R2290 |
T3572 |
T3571 |
prep |
with,consistent |
R2291 |
T3573 |
T3572 |
pobj |
preference,with |
R2292 |
T3574 |
T3573 |
prep |
of,preference |
R2293 |
T3575 |
T3574 |
pobj |
DNase,of |
R2294 |
T3576 |
T3575 |
nummod |
I,DNase |
R2295 |
T3577 |
T3573 |
prep |
to,preference |
R2296 |
T3578 |
T3579 |
amod |
double,stranded |
R2297 |
T3579 |
T3581 |
amod |
stranded,DNA |
R2298 |
T3580 |
T3579 |
punct |
-,stranded |
R2299 |
T3581 |
T3577 |
pobj |
DNA,to |
R2300 |
T3582 |
T3534 |
punct |
.,detected |
R2301 |
T3584 |
T3585 |
prep |
With,detected |
R2302 |
T3586 |
T3587 |
det |
the,substrate |
R2303 |
T3587 |
T3584 |
pobj |
substrate,With |
R2304 |
T3588 |
T3587 |
nmod |
Bub66,substrate |
R2305 |
T3589 |
T3588 |
punct |
/,Bub66 |
R2306 |
T3590 |
T3588 |
appos |
T,Bub66 |
R2307 |
T3591 |
T3590 |
punct |
-,T |
R2308 |
T3592 |
T3590 |
amod |
rich,T |
R2309 |
T3593 |
T3585 |
punct |
", ",detected |
R2310 |
T3594 |
T3595 |
amod |
strong,protection |
R2311 |
T3595 |
T3585 |
nsubjpass |
protection,detected |
R2312 |
T3596 |
T3585 |
auxpass |
was,detected |
R2313 |
T3597 |
T3585 |
prep |
on,detected |
R2314 |
T3598 |
T3599 |
det |
the,regions |
R2315 |
T3599 |
T3597 |
pobj |
regions,on |
R2316 |
T3600 |
T3599 |
amod |
entire,regions |
R2317 |
T3601 |
T3602 |
amod |
single,stranded |
R2318 |
T3602 |
T3599 |
amod |
stranded,regions |
R2319 |
T3603 |
T3602 |
punct |
-,stranded |
R2320 |
T3604 |
T3599 |
prep |
of,regions |
R2321 |
T3605 |
T3606 |
preconj |
both,top |
R2322 |
T3606 |
T3607 |
amod |
top,strands |
R2323 |
T3607 |
T3604 |
pobj |
strands,of |
R2324 |
T3608 |
T3606 |
cc |
and,top |
R2325 |
T3609 |
T3606 |
conj |
bottom,top |
R2326 |
T3610 |
T3585 |
prep |
with,detected |
R2327 |
T3611 |
T3612 |
amod |
increasing,concentration |
R2328 |
T3612 |
T3610 |
pobj |
concentration,with |
R2329 |
T3613 |
T3612 |
prep |
of,concentration |
R2330 |
T3614 |
T3613 |
pobj |
Mcm4,of |
R2331 |
T3615 |
T3614 |
punct |
/,Mcm4 |
R2332 |
T3616 |
T3614 |
nummod |
6,Mcm4 |
R2333 |
T3617 |
T3614 |
punct |
/,Mcm4 |
R2334 |
T3618 |
T3614 |
nummod |
7,Mcm4 |
R2335 |
T3619 |
T3585 |
punct |
", ",detected |
R2336 |
T3620 |
T3585 |
advcl |
consistent,detected |
R2337 |
T3621 |
T3620 |
prep |
with,consistent |
R2338 |
T3622 |
T3623 |
amod |
high,affinity |
R2339 |
T3623 |
T3621 |
pobj |
affinity,with |
R2340 |
T3624 |
T3623 |
prep |
of,affinity |
R2341 |
T3625 |
T3624 |
pobj |
Mcm4,of |
R2342 |
T3626 |
T3625 |
punct |
/,Mcm4 |
R2343 |
T3627 |
T3625 |
nummod |
6,Mcm4 |
R2344 |
T3628 |
T3625 |
punct |
/,Mcm4 |
R2345 |
T3629 |
T3625 |
nummod |
7,Mcm4 |
R2346 |
T3630 |
T3623 |
prep |
to,affinity |
R2347 |
T3631 |
T3632 |
nmod |
T,sequences |
R2348 |
T3632 |
T3630 |
pobj |
sequences,to |
R2349 |
T3633 |
T3631 |
punct |
-,T |
R2350 |
T3634 |
T3631 |
amod |
rich,T |
R2351 |
T3635 |
T3632 |
compound |
bubble,sequences |
R2352 |
T3636 |
T3637 |
punct |
(,1A |
R2353 |
T3637 |
T3585 |
parataxis |
1A,detected |
R2354 |
T3638 |
T3637 |
compound |
Figure,1A |
R2355 |
T3639 |
T3637 |
punct |
),1A |
R2356 |
T3640 |
T3585 |
punct |
.,detected |
R2357 |
T3642 |
T3643 |
amod |
Similar,protection |
R2358 |
T3643 |
T3648 |
nsubjpass |
protection,detected |
R2359 |
T3644 |
T3642 |
punct |
", ",Similar |
R2360 |
T3645 |
T3642 |
cc |
but,Similar |
R2361 |
T3646 |
T3647 |
advmod |
less,significant |
R2362 |
T3647 |
T3642 |
conj |
significant,Similar |
R2363 |
T3649 |
T3648 |
auxpass |
was,detected |
R2364 |
T3650 |
T3648 |
prep |
on,detected |
R2365 |
T3651 |
T3652 |
det |
the,segment |
R2366 |
T3652 |
T3650 |
pobj |
segment,on |
R2367 |
T3653 |
T3654 |
amod |
single,stranded |
R2368 |
T3654 |
T3652 |
amod |
stranded,segment |
R2369 |
T3655 |
T3654 |
punct |
-,stranded |
R2370 |
T3656 |
T3652 |
compound |
DNA,segment |
R2371 |
T3657 |
T3652 |
prep |
with,segment |
R2372 |
T3658 |
T3659 |
compound |
P1,nucleases |
R2373 |
T3659 |
T3657 |
pobj |
nucleases,with |
R2374 |
T3660 |
T3661 |
punct |
(,1A |
R2375 |
T3661 |
T3652 |
parataxis |
1A,segment |
R2376 |
T3662 |
T3661 |
compound |
Figure,1A |
R2377 |
T3663 |
T3661 |
punct |
),1A |
R2378 |
T3664 |
T3652 |
punct |
", ",segment |
R2379 |
T3665 |
T3666 |
dep |
which,is |
R2380 |
T3666 |
T3652 |
relcl |
is,segment |
R2381 |
T3667 |
T3666 |
acomp |
specific,is |
R2382 |
T3668 |
T3667 |
prep |
to,specific |
R2383 |
T3669 |
T3670 |
amod |
single,stranded |
R2384 |
T3670 |
T3672 |
amod |
stranded,DNA |
R2385 |
T3671 |
T3670 |
punct |
-,stranded |
R2386 |
T3672 |
T3668 |
pobj |
DNA,to |
R2387 |
T3673 |
T3648 |
punct |
.,detected |
R2388 |
T3675 |
T3676 |
det |
The,results |
R2389 |
T3676 |
T3677 |
nsubj |
results,indicate |
R2390 |
T3678 |
T3679 |
mark |
that,loaded |
R2391 |
T3679 |
T3677 |
ccomp |
loaded,indicate |
R2392 |
T3680 |
T3681 |
det |
the,complex |
R2393 |
T3681 |
T3679 |
nsubjpass |
complex,loaded |
R2394 |
T3682 |
T3681 |
compound |
Mcm,complex |
R2395 |
T3683 |
T3679 |
auxpass |
is,loaded |
R2396 |
T3684 |
T3679 |
prep |
onto,loaded |
R2397 |
T3685 |
T3686 |
compound |
bubble,DNA |
R2398 |
T3686 |
T3684 |
pobj |
DNA,onto |
R2399 |
T3687 |
T3679 |
prep |
through,loaded |
R2400 |
T3688 |
T3687 |
pobj |
binding,through |
R2401 |
T3689 |
T3688 |
prep |
to,binding |
R2402 |
T3690 |
T3691 |
det |
both,strands |
R2403 |
T3691 |
T3689 |
pobj |
strands,to |
R2404 |
T3692 |
T3691 |
prep |
of,strands |
R2405 |
T3693 |
T3694 |
det |
the,region |
R2406 |
T3694 |
T3692 |
pobj |
region,of |
R2407 |
T3695 |
T3696 |
amod |
single,stranded |
R2408 |
T3696 |
T3694 |
amod |
stranded,region |
R2409 |
T3697 |
T3696 |
punct |
-,stranded |
R2410 |
T3698 |
T3677 |
punct |
.,indicate |
R2411 |
T3700 |
T3701 |
amod |
Careful,examination |
R2412 |
T3701 |
T3702 |
nsubj |
examination,indicated |
R2413 |
T3703 |
T3701 |
prep |
of,examination |
R2414 |
T3704 |
T3705 |
nmod |
Mcm4,footprints |
R2415 |
T3705 |
T3703 |
pobj |
footprints,of |
R2416 |
T3706 |
T3704 |
punct |
/,Mcm4 |
R2417 |
T3707 |
T3704 |
nummod |
6,Mcm4 |
R2418 |
T3708 |
T3704 |
punct |
/,Mcm4 |
R2419 |
T3709 |
T3704 |
nummod |
7,Mcm4 |
R2420 |
T3710 |
T3705 |
prep |
on,footprints |
R2421 |
T3711 |
T3712 |
det |
a,bubble |
R2422 |
T3712 |
T3710 |
pobj |
bubble,on |
R2423 |
T3713 |
T3714 |
amod |
strong,protection |
R2424 |
T3714 |
T3702 |
dobj |
protection,indicated |
R2425 |
T3715 |
T3714 |
prep |
on,protection |
R2426 |
T3716 |
T3717 |
det |
the,half |
R2427 |
T3717 |
T3715 |
pobj |
half,on |
R2428 |
T3718 |
T3717 |
nummod |
5,half |
R2429 |
T3719 |
T3718 |
punct |
′,5 |
R2430 |
T3720 |
T3717 |
punct |
-,half |
R2431 |
T3721 |
T3717 |
prep |
of,half |
R2432 |
T3722 |
T3723 |
det |
the,strand |
R2433 |
T3723 |
T3721 |
pobj |
strand,of |
R2434 |
T3724 |
T3723 |
amod |
top,strand |
R2435 |
T3725 |
T3714 |
cc |
and,protection |
R2436 |
T3726 |
T3727 |
amod |
moderate,protection |
R2437 |
T3727 |
T3714 |
conj |
protection,protection |
R2438 |
T3728 |
T3727 |
prep |
on,protection |
R2439 |
T3729 |
T3730 |
det |
the,half |
R2440 |
T3730 |
T3728 |
pobj |
half,on |
R2441 |
T3731 |
T3730 |
amod |
remaining,half |
R2442 |
T3732 |
T3730 |
nummod |
3,half |
R2443 |
T3733 |
T3732 |
punct |
′,3 |
R2444 |
T3734 |
T3730 |
punct |
-,half |
R2445 |
T3735 |
T3728 |
punct |
", ",on |
R2446 |
T3736 |
T3737 |
advmod |
as,as |
R2447 |
T3737 |
T3728 |
cc |
as,on |
R2448 |
T3738 |
T3737 |
advmod |
well,as |
R2449 |
T3739 |
T3728 |
conj |
on,on |
R2450 |
T3740 |
T3741 |
amod |
several,segment |
R2451 |
T3741 |
T3739 |
pobj |
segment,on |
R2452 |
T3742 |
T3743 |
compound |
base,pair |
R2453 |
T3743 |
T3741 |
compound |
pair,segment |
R2454 |
T3744 |
T3743 |
punct |
-,pair |
R2455 |
T3745 |
T3741 |
compound |
duplex,segment |
R2456 |
T3746 |
T3741 |
amod |
adjacent,segment |
R2457 |
T3747 |
T3746 |
prep |
to,adjacent |
R2458 |
T3748 |
T3749 |
det |
the,point |
R2459 |
T3749 |
T3747 |
pobj |
point,to |
R2460 |
T3750 |
T3749 |
compound |
branch,point |
R2461 |
T3751 |
T3752 |
punct |
(,1A |
R2462 |
T3752 |
T3739 |
parataxis |
1A,on |
R2463 |
T3753 |
T3752 |
compound |
Figure,1A |
R2464 |
T3754 |
T3752 |
cc |
and,1A |
R2465 |
T3755 |
T3752 |
conj |
data,1A |
R2466 |
T3756 |
T3757 |
neg |
not,shown |
R2467 |
T3757 |
T3755 |
acl |
shown,data |
R2468 |
T3758 |
T3752 |
punct |
),1A |
R2469 |
T3759 |
T3702 |
punct |
.,indicated |
R2470 |
T3761 |
T3762 |
det |
A,pattern |
R2471 |
T3762 |
T3764 |
nsubjpass |
pattern,observed |
R2472 |
T3763 |
T3762 |
amod |
similar,pattern |
R2473 |
T3765 |
T3762 |
prep |
of,pattern |
R2474 |
T3766 |
T3765 |
pobj |
protection,of |
R2475 |
T3767 |
T3764 |
auxpass |
was,observed |
R2476 |
T3768 |
T3764 |
advmod |
also,observed |
R2477 |
T3769 |
T3764 |
prep |
on,observed |
R2478 |
T3770 |
T3771 |
det |
the,strand |
R2479 |
T3771 |
T3769 |
pobj |
strand,on |
R2480 |
T3772 |
T3771 |
amod |
bottom,strand |
R2481 |
T3773 |
T3764 |
punct |
.,observed |
R2482 |
T3775 |
T3776 |
nsubj |
This,suggests |
R2483 |
T3777 |
T3778 |
mark |
that,bind |
R2484 |
T3778 |
T3776 |
ccomp |
bind,suggests |
R2485 |
T3779 |
T3778 |
nsubj |
Mcm4,bind |
R2486 |
T3780 |
T3779 |
punct |
/,Mcm4 |
R2487 |
T3781 |
T3779 |
nummod |
6,Mcm4 |
R2488 |
T3782 |
T3779 |
punct |
/,Mcm4 |
R2489 |
T3783 |
T3779 |
nummod |
7,Mcm4 |
R2490 |
T3784 |
T3778 |
aux |
may,bind |
R2491 |
T3785 |
T3778 |
prep |
to,bind |
R2492 |
T3786 |
T3787 |
det |
a,substrate |
R2493 |
T3787 |
T3785 |
pobj |
substrate,to |
R2494 |
T3788 |
T3787 |
compound |
replication,substrate |
R2495 |
T3789 |
T3787 |
compound |
bubble,substrate |
R2496 |
T3790 |
T3787 |
prep |
with,substrate |
R2497 |
T3791 |
T3792 |
det |
a,symmetry |
R2498 |
T3792 |
T3790 |
pobj |
symmetry,with |
R2499 |
T3793 |
T3792 |
nummod |
2-fold,symmetry |
R2500 |
T3794 |
T3778 |
prep |
as,bind |
R2501 |
T3795 |
T3796 |
det |
a,hexamer |
R2502 |
T3796 |
T3794 |
pobj |
hexamer,as |
R2503 |
T3797 |
T3796 |
amod |
double,hexamer |
R2504 |
T3798 |
T3799 |
punct |
(,1A |
R2505 |
T3799 |
T3796 |
parataxis |
1A,hexamer |
R2506 |
T3800 |
T3799 |
compound |
Figure,1A |
R2507 |
T3801 |
T3799 |
punct |
", ",1A |
R2508 |
T3802 |
T3799 |
advcl |
see,1A |
R2509 |
T3803 |
T3804 |
det |
the,scheme |
R2510 |
T3804 |
T3802 |
dobj |
scheme,see |
R2511 |
T3805 |
T3804 |
amod |
lower,scheme |
R2512 |
T3806 |
T3799 |
punct |
),1A |
R2513 |
T3807 |
T3778 |
punct |
", ",bind |
R2514 |
T3808 |
T3778 |
prep |
in,bind |
R2515 |
T3809 |
T3810 |
det |
a,manner |
R2516 |
T3810 |
T3808 |
pobj |
manner,in |
R2517 |
T3811 |
T3810 |
amod |
similar,manner |
R2518 |
T3812 |
T3811 |
prep |
to,similar |
R2519 |
T3813 |
T3814 |
compound |
SV40,antigen |
R2520 |
T3814 |
T3812 |
pobj |
antigen,to |
R2521 |
T3815 |
T3814 |
compound |
T,antigen |
R2522 |
T3816 |
T3814 |
punct |
-,antigen |
R2523 |
T3817 |
T3818 |
punct |
(,21 |
R2524 |
T3818 |
T3778 |
parataxis |
21,bind |
R2525 |
T3819 |
T3818 |
punct |
),21 |
R2526 |
T3820 |
T3776 |
punct |
.,suggests |
R2527 |
T3822 |
T3823 |
compound |
Nuclease,protection |
R2528 |
T3823 |
T3824 |
compound |
protection,assays |
R2529 |
T3824 |
T3825 |
nsubjpass |
assays,conducted |
R2530 |
T3826 |
T3825 |
auxpass |
were,conducted |
R2531 |
T3827 |
T3825 |
advmod |
also,conducted |
R2532 |
T3828 |
T3825 |
prep |
on,conducted |
R2533 |
T3829 |
T3830 |
det |
a,structure |
R2534 |
T3830 |
T3828 |
pobj |
structure,on |
R2535 |
T3831 |
T3832 |
npadvmod |
T,tailed |
R2536 |
T3832 |
T3830 |
amod |
tailed,structure |
R2537 |
T3833 |
T3832 |
punct |
-,tailed |
R2538 |
T3834 |
T3835 |
compound |
Y,fork |
R2539 |
T3835 |
T3830 |
compound |
fork,structure |
R2540 |
T3836 |
T3835 |
punct |
-,fork |
R2541 |
T3837 |
T3838 |
punct |
(,1B |
R2542 |
T3838 |
T3825 |
parataxis |
1B,conducted |
R2543 |
T3839 |
T3838 |
compound |
Figure,1B |
R2544 |
T3840 |
T3838 |
punct |
),1B |
R2545 |
T3841 |
T3825 |
punct |
.,conducted |
R2546 |
T3843 |
T3844 |
preconj |
Not,region |
R2547 |
T3844 |
T3851 |
nsubjpass |
region,protected |
R2548 |
T3845 |
T3843 |
advmod |
only,Not |
R2549 |
T3846 |
T3844 |
det |
the,region |
R2550 |
T3847 |
T3848 |
amod |
single,stranded |
R2551 |
T3848 |
T3844 |
amod |
stranded,region |
R2552 |
T3849 |
T3848 |
punct |
-,stranded |
R2553 |
T3850 |
T3844 |
compound |
tail,region |
R2554 |
T3852 |
T3844 |
cc |
but,region |
R2555 |
T3853 |
T3852 |
advmod |
also,but |
R2556 |
T3854 |
T3855 |
det |
the,DNA |
R2557 |
T3855 |
T3844 |
conj |
DNA,region |
R2558 |
T3856 |
T3857 |
nummod |
7,bp |
R2559 |
T3857 |
T3855 |
compound |
bp,DNA |
R2560 |
T3858 |
T3855 |
compound |
duplex,DNA |
R2561 |
T3859 |
T3855 |
amod |
adjacent,DNA |
R2562 |
T3860 |
T3859 |
prep |
to,adjacent |
R2563 |
T3861 |
T3862 |
det |
the,junction |
R2564 |
T3862 |
T3860 |
pobj |
junction,to |
R2565 |
T3863 |
T3862 |
nmod |
duplex,junction |
R2566 |
T3864 |
T3863 |
punct |
-,duplex |
R2567 |
T3865 |
T3863 |
prep |
to,duplex |
R2568 |
T3866 |
T3865 |
punct |
-,to |
R2569 |
T3867 |
T3868 |
amod |
single,strand |
R2570 |
T3868 |
T3865 |
pobj |
strand,to |
R2571 |
T3869 |
T3868 |
punct |
-,strand |
R2572 |
T3870 |
T3851 |
auxpass |
were,protected |
R2573 |
T3871 |
T3851 |
agent |
by,protected |
R2574 |
T3872 |
T3873 |
compound |
Mcm,binding |
R2575 |
T3873 |
T3871 |
pobj |
binding,by |
R2576 |
T3874 |
T3873 |
prep |
from,binding |
R2577 |
T3875 |
T3876 |
compound |
DNaseI,digestion |
R2578 |
T3876 |
T3874 |
pobj |
digestion,from |
R2579 |
T3877 |
T3851 |
punct |
.,protected |
R2580 |
T3879 |
T3880 |
det |
These,results |
R2581 |
T3880 |
T3881 |
nsubj |
results,indicate |
R2582 |
T3882 |
T3883 |
mark |
that,interacts |
R2583 |
T3883 |
T3881 |
advcl |
interacts,indicate |
R2584 |
T3884 |
T3885 |
nmod |
Mcm4,protein |
R2585 |
T3885 |
T3883 |
nsubj |
protein,interacts |
R2586 |
T3886 |
T3884 |
punct |
/,Mcm4 |
R2587 |
T3887 |
T3884 |
nummod |
6,Mcm4 |
R2588 |
T3888 |
T3884 |
punct |
/,Mcm4 |
R2589 |
T3889 |
T3884 |
nummod |
7,Mcm4 |
R2590 |
T3890 |
T3883 |
advmod |
primarily,interacts |
R2591 |
T3891 |
T3883 |
prep |
with,interacts |
R2592 |
T3892 |
T3893 |
amod |
single,stranded |
R2593 |
T3893 |
T3895 |
amod |
stranded,region |
R2594 |
T3894 |
T3893 |
punct |
-,stranded |
R2595 |
T3895 |
T3891 |
pobj |
region,with |
R2596 |
T3896 |
T3895 |
compound |
DNA,region |
R2597 |
T3897 |
T3883 |
cc |
and,interacts |
R2598 |
T3898 |
T3899 |
mark |
that,spans |
R2599 |
T3899 |
T3883 |
conj |
spans,interacts |
R2600 |
T3900 |
T3901 |
det |
the,interaction |
R2601 |
T3901 |
T3899 |
nsubj |
interaction,spans |
R2602 |
T3902 |
T3899 |
advmod |
also,spans |
R2603 |
T3903 |
T3904 |
det |
the,segments |
R2604 |
T3904 |
T3899 |
dobj |
segments,spans |
R2605 |
T3905 |
T3904 |
compound |
duplex,segments |
R2606 |
T3906 |
T3904 |
prep |
near,segments |
R2607 |
T3907 |
T3908 |
det |
the,branchpoint |
R2608 |
T3908 |
T3906 |
pobj |
branchpoint,near |
R2609 |
T3909 |
T3908 |
prep |
of,branchpoint |
R2610 |
T3910 |
T3911 |
det |
the,fork |
R2611 |
T3911 |
T3909 |
pobj |
fork,of |
R2612 |
T3912 |
T3911 |
compound |
replication,fork |
R2613 |
T3913 |
T3881 |
punct |
.,indicate |