PMC:1140370 JSON TXT

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Id	Subject	Object	Predicate	Lexical cue
T143	4-11	NN	denotes	binding
T144	0-59	sentence	denotes	DNA binding and helicase actions of mouse MCM4/6/7 helicase
T145	25-32	NNS	denotes	actions
T146	12-15	CC	denotes	and
T147	16-24	NN	denotes	helicase
T148	33-35	IN	denotes	of
T149	36-41	NN	denotes	mouse
T150	51-59	NN	denotes	helicase
T151	42-46	NN	denotes	MCM4
T152	46-47	HYPH	denotes	/
T153	47-48	CD	denotes	6
T154	48-49	HYPH	denotes	/
T155	49-50	CD	denotes	7
T156	59-60	sentence	denotes
T157	70-147	sentence	denotes	Helicases play central roles in initiation and elongation of DNA replication.
T158	70-79	NNS	denotes	Helicases
T159	80-84	VBP	denotes	play
T160	85-92	JJ	denotes	central
T161	93-98	NNS	denotes	roles
T162	99-101	IN	denotes	in
T163	102-112	NN	denotes	initiation
T164	113-116	CC	denotes	and
T165	117-127	NN	denotes	elongation
T166	128-130	IN	denotes	of
T167	131-134	NN	denotes	DNA
T168	135-146	NN	denotes	replication
T169	146-147	.	denotes	.
T170	147-304	sentence	denotes	We previously reported that helicase and ATPase activities of the mammalian Mcm4/6/7 complex are activated specifically by thymine-rich single-stranded DNA.
T171	148-150	PRP	denotes	We
T172	162-170	VBD	denotes	reported
T173	151-161	RB	denotes	previously
T174	171-175	IN	denotes	that
T175	245-254	VBN	denotes	activated
T176	176-184	NN	denotes	helicase
T177	196-206	NNS	denotes	activities
T178	185-188	CC	denotes	and
T179	189-195	NN	denotes	ATPase
T180	207-209	IN	denotes	of
T181	210-213	DT	denotes	the
T182	233-240	NN	denotes	complex
T183	214-223	JJ	denotes	mammalian
T184	224-228	NN	denotes	Mcm4
T185	228-229	HYPH	denotes	/
T186	229-230	CD	denotes	6
T187	230-231	HYPH	denotes	/
T188	231-232	CD	denotes	7
T189	241-244	VBP	denotes	are
T190	255-267	RB	denotes	specifically
T191	268-270	IN	denotes	by
T192	271-278	NN	denotes	thymine
T193	279-283	JJ	denotes	rich
T194	278-279	HYPH	denotes	-
T195	300-303	NN	denotes	DNA
T196	284-290	JJ	denotes	single
T197	291-299	VBN	denotes	stranded
T198	290-291	HYPH	denotes	-
T199	303-304	.	denotes	.
T200	304-398	sentence	denotes	Here, we examined its substrate preference and helicase actions using various synthetic DNAs.
T201	305-309	RB	denotes	Here
T202	314-322	VBD	denotes	examined
T203	309-311	,	denotes	,
T204	311-313	PRP	denotes	we
T205	323-326	PRP$	denotes	its
T206	337-347	NN	denotes	preference
T207	327-336	NN	denotes	substrate
T208	348-351	CC	denotes	and
T209	352-360	NN	denotes	helicase
T210	361-368	NNS	denotes	actions
T211	369-374	VBG	denotes	using
T212	375-382	JJ	denotes	various
T213	393-397	NNS	denotes	DNAs
T214	383-392	JJ	denotes	synthetic
T215	397-398	.	denotes	.
T216	398-582	sentence	denotes	On a bubble substrate, Mcm4/6/7 makes symmetric dual contacts with the 5′-proximal 25 nt single-stranded segments adjacent to the branch points, presumably generating double hexamers.
T217	399-401	IN	denotes	On
T218	431-436	VBZ	denotes	makes
T219	402-403	DT	denotes	a
T220	411-420	NN	denotes	substrate
T221	404-410	NN	denotes	bubble
T222	420-422	,	denotes	,
T223	422-426	NN	denotes	Mcm4
T224	426-427	HYPH	denotes	/
T225	427-428	CD	denotes	6
T226	428-429	HYPH	denotes	/
T227	429-430	CD	denotes	7
T228	437-446	JJ	denotes	symmetric
T229	452-460	NNS	denotes	contacts
T230	447-451	JJ	denotes	dual
T231	461-465	IN	denotes	with
T232	466-469	DT	denotes	the
T233	504-512	NNS	denotes	segments
T234	470-471	CD	denotes	5
T235	473-481	JJ	denotes	proximal
T236	471-472	SYM	denotes	′
T237	472-473	HYPH	denotes	-
T238	482-484	CD	denotes	25
T239	485-487	NN	denotes	nt
T240	488-494	JJ	denotes	single
T241	495-503	VBN	denotes	stranded
T242	494-495	HYPH	denotes	-
T243	513-521	JJ	denotes	adjacent
T244	522-524	IN	denotes	to
T245	525-528	DT	denotes	the
T246	536-542	NNS	denotes	points
T247	529-535	NN	denotes	branch
T248	542-544	,	denotes	,
T249	544-554	RB	denotes	presumably
T250	555-565	VBG	denotes	generating
T251	566-572	JJ	denotes	double
T252	573-581	NNS	denotes	hexamers
T253	581-582	.	denotes	.
T254	582-828	sentence	denotes	Loss of thymine residues from one single-strand results in significant decrease of unwinding efficacy, suggesting that concurrent bidirectional unwinding by a single double hexameric Mcm4/6/7 may play a role in efficient unwinding of the bubble.
T255	583-587	NN	denotes	Loss
T256	631-638	VBZ	denotes	results
T257	588-590	IN	denotes	of
T258	591-598	NN	denotes	thymine
T259	599-607	NNS	denotes	residues
T260	608-612	IN	denotes	from
T261	613-616	CD	denotes	one
T262	624-630	NN	denotes	strand
T263	617-623	JJ	denotes	single
T264	623-624	HYPH	denotes	-
T265	639-641	IN	denotes	in
T266	642-653	JJ	denotes	significant
T267	654-662	NN	denotes	decrease
T268	663-665	IN	denotes	of
T269	666-675	VBG	denotes	unwinding
T270	676-684	NN	denotes	efficacy
T271	684-686	,	denotes	,
T272	686-696	VBG	denotes	suggesting
T273	697-701	IN	denotes	that
T274	779-783	VB	denotes	play
T275	702-712	JJ	denotes	concurrent
T276	727-736	NN	denotes	unwinding
T277	713-726	JJ	denotes	bidirectional
T278	737-739	IN	denotes	by
T279	740-741	DT	denotes	a
T280	766-770	NN	denotes	Mcm4
T281	742-748	JJ	denotes	single
T282	749-755	JJ	denotes	double
T283	756-765	JJ	denotes	hexameric
T284	770-771	HYPH	denotes	/
T285	771-772	CD	denotes	6
T286	772-773	HYPH	denotes	/
T287	773-774	CD	denotes	7
T288	775-778	MD	denotes	may
T289	784-785	DT	denotes	a
T290	786-790	NN	denotes	role
T291	791-793	IN	denotes	in
T292	794-803	JJ	denotes	efficient
T293	804-813	NN	denotes	unwinding
T294	814-816	IN	denotes	of
T295	817-820	DT	denotes	the
T296	821-827	NN	denotes	bubble
T297	827-828	.	denotes	.
T298	828-933	sentence	denotes	Mcm4/6/7 binds and unwinds various fork and extension structures carrying a single-stranded 3′-tail DNA.
T299	829-833	NN	denotes	Mcm4
T300	838-843	VBZ	denotes	binds
T301	833-834	HYPH	denotes	/
T302	834-835	CD	denotes	6
T303	835-836	HYPH	denotes	/
T304	836-837	CD	denotes	7
T305	844-847	CC	denotes	and
T306	848-855	VBZ	denotes	unwinds
T307	856-863	JJ	denotes	various
T308	883-893	NNS	denotes	structures
T309	864-868	NN	denotes	fork
T310	869-872	CC	denotes	and
T311	873-882	NN	denotes	extension
T312	894-902	VBG	denotes	carrying
T313	903-904	DT	denotes	a
T314	929-932	NN	denotes	DNA
T315	905-911	JJ	denotes	single
T316	912-920	VBN	denotes	stranded
T317	911-912	HYPH	denotes	-
T318	921-922	CD	denotes	3
T319	924-928	NN	denotes	tail
T320	922-923	SYM	denotes	′
T321	923-924	HYPH	denotes	-
T322	932-933	.	denotes	.
T323	933-1090	sentence	denotes	The extent of helicase activation depends on the sequence context of the 3′-tail, and the maximum level is achieved by DNA with 50% or more thymine content.
T324	934-937	DT	denotes	The
T325	938-944	NN	denotes	extent
T326	968-975	VBZ	denotes	depends
T327	945-947	IN	denotes	of
T328	948-956	NN	denotes	helicase
T329	957-967	NN	denotes	activation
T330	976-978	IN	denotes	on
T331	979-982	DT	denotes	the
T332	992-999	NN	denotes	context
T333	983-991	NN	denotes	sequence
T334	1000-1002	IN	denotes	of
T335	1003-1006	DT	denotes	the
T336	1010-1014	NN	denotes	tail
T337	1007-1008	CD	denotes	3
T338	1008-1009	SYM	denotes	′
T339	1009-1010	HYPH	denotes	-
T340	1014-1016	,	denotes	,
T341	1016-1019	CC	denotes	and
T342	1020-1023	DT	denotes	the
T343	1032-1037	NN	denotes	level
T344	1024-1031	JJ	denotes	maximum
T345	1041-1049	VBN	denotes	achieved
T346	1038-1040	VBZ	denotes	is
T347	1050-1052	IN	denotes	by
T348	1053-1056	NN	denotes	DNA
T349	1057-1061	IN	denotes	with
T350	1062-1064	CD	denotes	50
T351	1064-1065	NN	denotes	%
T352	1082-1089	NN	denotes	content
T353	1066-1068	CC	denotes	or
T354	1069-1073	JJR	denotes	more
T355	1074-1081	NN	denotes	thymine
T356	1089-1090	.	denotes	.
T357	1090-1186	sentence	denotes	Strand displacement by Mcm4/6/7 is inhibited, as the GC content of the duplex region increases.
T358	1091-1097	NN	denotes	Strand
T359	1098-1110	NN	denotes	displacement
T360	1126-1135	VBN	denotes	inhibited
T361	1111-1113	IN	denotes	by
T362	1114-1118	NN	denotes	Mcm4
T363	1118-1119	HYPH	denotes	/
T364	1119-1120	CD	denotes	6
T365	1120-1121	HYPH	denotes	/
T366	1121-1122	CD	denotes	7
T367	1123-1125	VBZ	denotes	is
T368	1135-1137	,	denotes	,
T369	1137-1139	IN	denotes	as
T370	1176-1185	VBZ	denotes	increases
T371	1140-1143	DT	denotes	the
T372	1147-1154	NN	denotes	content
T373	1144-1146	NN	denotes	GC
T374	1155-1157	IN	denotes	of
T375	1158-1161	DT	denotes	the
T376	1169-1175	NN	denotes	region
T377	1162-1168	NN	denotes	duplex
T378	1185-1186	.	denotes	.
T379	1186-1387	sentence	denotes	Replacement of cytosine–guanine pairs with cytosine–inosine pairs in the duplex restored unwinding, suggesting that mammalian Mcm4/6/7 helicase has difficulties in unwinding stably base-paired duplex.
T380	1187-1198	NN	denotes	Replacement
T381	1267-1275	VBD	denotes	restored
T382	1199-1201	IN	denotes	of
T383	1202-1210	NN	denotes	cytosine
T384	1211-1218	NN	denotes	guanine
T385	1210-1211	HYPH	denotes	–
T386	1219-1224	NNS	denotes	pairs
T387	1225-1229	IN	denotes	with
T388	1230-1238	NN	denotes	cytosine
T389	1239-1246	NN	denotes	inosine
T390	1238-1239	HYPH	denotes	–
T391	1247-1252	NNS	denotes	pairs
T392	1253-1255	IN	denotes	in
T393	1256-1259	DT	denotes	the
T394	1260-1266	NN	denotes	duplex
T395	1276-1285	NN	denotes	unwinding
T396	1285-1287	,	denotes	,
T397	1287-1297	VBG	denotes	suggesting
T398	1298-1302	IN	denotes	that
T399	1331-1334	VBZ	denotes	has
T400	1303-1312	JJ	denotes	mammalian
T401	1322-1330	NN	denotes	helicase
T402	1313-1317	NN	denotes	Mcm4
T403	1317-1318	HYPH	denotes	/
T404	1318-1319	CD	denotes	6
T405	1319-1320	HYPH	denotes	/
T406	1320-1321	CD	denotes	7
T407	1335-1347	NNS	denotes	difficulties
T408	1348-1350	IN	denotes	in
T409	1351-1360	VBG	denotes	unwinding
T410	1361-1367	RB	denotes	stably
T411	1373-1379	VBN	denotes	paired
T412	1368-1372	NN	denotes	base
T413	1372-1373	HYPH	denotes	-
T414	1380-1386	NN	denotes	duplex
T415	1386-1387	.	denotes	.
T416	1387-1523	sentence	denotes	Taken together, these findings reveal important features on activation and substrate preference of the eukaryotic replicative helicase.
T417	1388-1393	VBN	denotes	Taken
T418	1419-1425	VBP	denotes	reveal
T419	1394-1402	RB	denotes	together
T420	1402-1404	,	denotes	,
T421	1404-1409	DT	denotes	these
T422	1410-1418	NNS	denotes	findings
T423	1426-1435	JJ	denotes	important
T424	1436-1444	NNS	denotes	features
T425	1445-1447	IN	denotes	on
T426	1448-1458	NN	denotes	activation
T427	1473-1483	NN	denotes	preference
T428	1459-1462	CC	denotes	and
T429	1463-1472	NN	denotes	substrate
T430	1484-1486	IN	denotes	of
T431	1487-1490	DT	denotes	the
T432	1514-1522	NN	denotes	helicase
T433	1491-1501	JJ	denotes	eukaryotic
T434	1502-1513	JJ	denotes	replicative
T435	1522-1523	.	denotes	.
T757	1538-1541	NN	denotes	DNA
T758	1542-1551	NNS	denotes	helicases
T759	1552-1558	VBP	denotes	couple
T760	1559-1562	DT	denotes	the
T761	1563-1573	NN	denotes	hydrolysis
T762	1574-1576	IN	denotes	of
T763	1577-1587	NN	denotes	nucleotide
T764	1607-1619	NN	denotes	triphosphate
T765	1588-1590	CC	denotes	or
T766	1591-1606	NN	denotes	deoxynucleotide
T767	1620-1622	IN	denotes	to
T768	1623-1629	NN	denotes	duplex
T769	1630-1639	NN	denotes	unwinding
T770	1640-1641	-LRB-	denotes	(
T771	1641-1642	CD	denotes	1
T772	1642-1643	-RRB-	denotes	)
T773	1643-1644	.	denotes	.
T774	1644-1791	sentence	denotes	Coordination of a set of subactivities including nucleotide binding, DNA binding and ATP hydrolysis is required for unwinding of duplex DNA (1,2).
T775	1645-1657	NN	denotes	Coordination
T776	1748-1756	VBN	denotes	required
T777	1658-1660	IN	denotes	of
T778	1661-1662	DT	denotes	a
T779	1663-1666	NN	denotes	set
T780	1667-1669	IN	denotes	of
T781	1670-1683	NNS	denotes	subactivities
T782	1684-1693	VBG	denotes	including
T783	1694-1704	NN	denotes	nucleotide
T784	1705-1712	NN	denotes	binding
T785	1712-1714	,	denotes	,
T786	1714-1717	NN	denotes	DNA
T787	1718-1725	NN	denotes	binding
T788	1726-1729	CC	denotes	and
T789	1730-1733	NN	denotes	ATP
T790	1734-1744	NN	denotes	hydrolysis
T791	1745-1747	VBZ	denotes	is
T792	1757-1760	IN	denotes	for
T793	1761-1770	NN	denotes	unwinding
T794	1771-1773	IN	denotes	of
T795	1774-1780	NN	denotes	duplex
T796	1781-1784	NN	denotes	DNA
T797	1785-1786	-LRB-	denotes	(
T798	1788-1789	CD	denotes	2
T799	1786-1787	CD	denotes	1
T800	1787-1788	,	denotes	,
T801	1789-1790	-RRB-	denotes	)
T802	1790-1791	.	denotes	.
T803	1791-1927	sentence	denotes	The hexameric helicases, to which minichromosome maintenance (Mcm) helicase belongs, are known to assemble a ring-like structure (3–5).
T804	1792-1795	DT	denotes	The
T805	1806-1815	NNS	denotes	helicases
T806	1796-1805	JJ	denotes	hexameric
T807	1881-1886	VBN	denotes	known
T808	1815-1817	,	denotes	,
T809	1817-1819	IN	denotes	to
T810	1868-1875	VBZ	denotes	belongs
T811	1820-1825	WDT	denotes	which
T812	1826-1840	NN	denotes	minichromosome
T813	1841-1852	NN	denotes	maintenance
T814	1859-1867	NN	denotes	helicase
T815	1853-1854	-LRB-	denotes	(
T816	1854-1857	NN	denotes	Mcm
T817	1857-1858	-RRB-	denotes	)
T818	1875-1877	,	denotes	,
T819	1877-1880	VBP	denotes	are
T820	1887-1889	TO	denotes	to
T821	1890-1898	VB	denotes	assemble
T822	1899-1900	DT	denotes	a
T823	1911-1920	NN	denotes	structure
T824	1901-1905	NN	denotes	ring
T825	1906-1910	JJ	denotes	like
T826	1905-1906	HYPH	denotes	-
T827	1921-1922	-LRB-	denotes	(
T828	1922-1923	CD	denotes	3
T829	1923-1924	SYM	denotes	–
T830	1924-1925	CD	denotes	5
T831	1925-1926	-RRB-	denotes	)
T832	1926-1927	.	denotes	.
T833	1927-2197	sentence	denotes	Whereas most of DNA helicases of this group, including simian virus 40 large T-antigen (6), Escherichia coli DnaB (7) and Archaeal Mcm (4,8,9), are composed of a single subunit, the active eukaryotic Mcm helicases are composed of two trimeric Mcm4/6/7 proteins (10–14).
T834	1928-1935	IN	denotes	Whereas
T835	2076-2084	VBN	denotes	composed
T836	1936-1940	JJS	denotes	most
T837	1941-1943	IN	denotes	of
T838	1944-1947	NN	denotes	DNA
T839	1948-1957	NNS	denotes	helicases
T840	1958-1960	IN	denotes	of
T841	1961-1965	DT	denotes	this
T842	1966-1971	NN	denotes	group
T843	1971-1973	,	denotes	,
T844	1973-1982	VBG	denotes	including
T845	1983-1989	JJ	denotes	simian
T846	1990-1995	NN	denotes	virus
T847	2007-2014	NN	denotes	antigen
T848	1996-1998	CD	denotes	40
T849	1999-2004	JJ	denotes	large
T850	2005-2006	NN	denotes	T
T851	2006-2007	HYPH	denotes	-
T852	2015-2016	-LRB-	denotes	(
T853	2016-2017	CD	denotes	6
T854	2017-2018	-RRB-	denotes	)
T855	2018-2020	,	denotes	,
T856	2020-2031	NNP	denotes	Escherichia
T857	2032-2036	NNP	denotes	coli
T858	2037-2041	NN	denotes	DnaB
T859	2042-2043	-LRB-	denotes	(
T860	2043-2044	CD	denotes	7
T861	2044-2045	-RRB-	denotes	)
T862	2046-2049	CC	denotes	and
T863	2050-2058	JJ	denotes	Archaeal
T864	2059-2062	NN	denotes	Mcm
T865	2063-2064	-LRB-	denotes	(
T866	2068-2069	CD	denotes	9
T867	2064-2065	CD	denotes	4
T868	2065-2066	,	denotes	,
T869	2066-2067	CD	denotes	8
T870	2067-2068	,	denotes	,
T871	2069-2070	-RRB-	denotes	)
T872	2070-2072	,	denotes	,
T873	2072-2075	VBP	denotes	are
T874	2146-2154	VBN	denotes	composed
T875	2085-2087	IN	denotes	of
T876	2088-2089	DT	denotes	a
T877	2097-2104	NN	denotes	subunit
T878	2090-2096	JJ	denotes	single
T879	2104-2106	,	denotes	,
T880	2106-2109	DT	denotes	the
T881	2132-2141	NNS	denotes	helicases
T882	2110-2116	JJ	denotes	active
T883	2117-2127	JJ	denotes	eukaryotic
T884	2128-2131	NN	denotes	Mcm
T885	2142-2145	VBP	denotes	are
T886	2155-2157	IN	denotes	of
T887	2158-2161	CD	denotes	two
T888	2180-2188	NN	denotes	proteins
T889	2162-2170	JJ	denotes	trimeric
T890	2171-2175	NN	denotes	Mcm4
T891	2175-2176	HYPH	denotes	/
T892	2176-2177	CD	denotes	6
T893	2177-2178	HYPH	denotes	/
T894	2178-2179	CD	denotes	7
T895	2189-2190	-LRB-	denotes	(
T896	2190-2192	CD	denotes	10
T897	2192-2193	SYM	denotes	–
T898	2193-2195	CD	denotes	14
T899	2195-2196	-RRB-	denotes	)
T900	2196-2197	.	denotes	.
T901	2197-2313	sentence	denotes	All the six Mcm 2–7 proteins contain highly conserved DNA-dependent ATPase motifs in their central regions (15,16).
T902	2198-2201	PDT	denotes	All
T903	2218-2226	NN	denotes	proteins
T904	2202-2205	DT	denotes	the
T905	2206-2209	CD	denotes	six
T906	2210-2213	NN	denotes	Mcm
T907	2214-2215	CD	denotes	2
T908	2215-2216	SYM	denotes	–
T909	2216-2217	CD	denotes	7
T910	2227-2234	VBP	denotes	contain
T911	2235-2241	RB	denotes	highly
T912	2242-2251	VBN	denotes	conserved
T913	2273-2279	NNS	denotes	motifs
T914	2252-2255	NN	denotes	DNA
T915	2256-2265	JJ	denotes	dependent
T916	2255-2256	HYPH	denotes	-
T917	2266-2272	NN	denotes	ATPase
T918	2280-2282	IN	denotes	in
T919	2283-2288	PRP$	denotes	their
T920	2297-2304	NNS	denotes	regions
T921	2289-2296	JJ	denotes	central
T922	2305-2306	-LRB-	denotes	(
T923	2309-2311	CD	denotes	16
T924	2306-2308	CD	denotes	15
T925	2308-2309	,	denotes	,
T926	2311-2312	-RRB-	denotes	)
T927	2312-2313	.	denotes	.
T928	2313-2486	sentence	denotes	Among the several stable subcomplexes which MCM proteins can generate, only the Mcm4/6/7 complex has been shown to possess an intrinsic DNA helicase activity (10–14,17–18).
T929	2314-2319	IN	denotes	Among
T930	2420-2425	VBN	denotes	shown
T931	2320-2323	DT	denotes	the
T932	2339-2351	NNS	denotes	subcomplexes
T933	2324-2331	JJ	denotes	several
T934	2332-2338	JJ	denotes	stable
T935	2352-2357	WDT	denotes	which
T936	2375-2383	VB	denotes	generate
T937	2358-2361	NN	denotes	MCM
T938	2362-2370	NN	denotes	proteins
T939	2371-2374	MD	denotes	can
T940	2383-2385	,	denotes	,
T941	2385-2389	RB	denotes	only
T942	2403-2410	NN	denotes	complex
T943	2390-2393	DT	denotes	the
T944	2394-2398	NN	denotes	Mcm4
T945	2398-2399	HYPH	denotes	/
T946	2399-2400	CD	denotes	6
T947	2400-2401	HYPH	denotes	/
T948	2401-2402	CD	denotes	7
T949	2411-2414	VBZ	denotes	has
T950	2415-2419	VBN	denotes	been
T951	2426-2428	TO	denotes	to
T952	2429-2436	VB	denotes	possess
T953	2437-2439	DT	denotes	an
T954	2463-2471	NN	denotes	activity
T955	2440-2449	JJ	denotes	intrinsic
T956	2450-2453	NN	denotes	DNA
T957	2454-2462	NN	denotes	helicase
T958	2472-2473	-LRB-	denotes	(
T959	2473-2475	CD	denotes	10
T960	2475-2476	SYM	denotes	–
T961	2476-2478	CD	denotes	14
T962	2478-2479	,	denotes	,
T963	2479-2481	CD	denotes	17
T964	2481-2482	SYM	denotes	–
T965	2482-2484	CD	denotes	18
T966	2484-2485	-RRB-	denotes	)
T967	2485-2486	.	denotes	.
T968	2486-2755	sentence	denotes	While Mcm4, Mcm6 and Mcm7 proteins make distinct contribution to its helicase activity (11–13), Mcm2 or Mcm3/5 inhibit the helicase activity of the Mcm4/6/7 complex by converting its double trimer structure into a heterotetramer or heteropentamer, respectively (3,11).
T969	2487-2492	IN	denotes	While
T970	2522-2526	VBP	denotes	make
T971	2493-2497	NN	denotes	Mcm4
T972	2513-2521	NN	denotes	proteins
T973	2497-2499	,	denotes	,
T974	2499-2503	NN	denotes	Mcm6
T975	2504-2507	CC	denotes	and
T976	2508-2512	NN	denotes	Mcm7
T977	2598-2605	VBP	denotes	inhibit
T978	2527-2535	JJ	denotes	distinct
T979	2536-2548	NN	denotes	contribution
T980	2549-2551	IN	denotes	to
T981	2552-2555	PRP$	denotes	its
T982	2565-2573	NN	denotes	activity
T983	2556-2564	NN	denotes	helicase
T984	2574-2575	-LRB-	denotes	(
T985	2575-2577	CD	denotes	11
T986	2577-2578	SYM	denotes	–
T987	2578-2580	CD	denotes	13
T988	2580-2581	-RRB-	denotes	)
T989	2581-2583	,	denotes	,
T990	2583-2587	NN	denotes	Mcm2
T991	2588-2590	CC	denotes	or
T992	2591-2595	NN	denotes	Mcm3
T993	2595-2596	HYPH	denotes	/
T994	2596-2597	CD	denotes	5
T995	2606-2609	DT	denotes	the
T996	2619-2627	NN	denotes	activity
T997	2610-2618	NN	denotes	helicase
T998	2628-2630	IN	denotes	of
T999	2631-2634	DT	denotes	the
T1000	2644-2651	NN	denotes	complex
T1001	2635-2639	NN	denotes	Mcm4
T1002	2639-2640	HYPH	denotes	/
T1003	2640-2641	CD	denotes	6
T1004	2641-2642	HYPH	denotes	/
T1005	2642-2643	CD	denotes	7
T1006	2652-2654	IN	denotes	by
T1007	2655-2665	VBG	denotes	converting
T1008	2666-2669	PRP$	denotes	its
T1009	2684-2693	NN	denotes	structure
T1010	2670-2676	JJ	denotes	double
T1011	2677-2683	NN	denotes	trimer
T1012	2694-2698	IN	denotes	into
T1013	2699-2700	DT	denotes	a
T1014	2701-2715	NN	denotes	heterotetramer
T1015	2716-2718	CC	denotes	or
T1016	2719-2733	NN	denotes	heteropentamer
T1017	2733-2735	,	denotes	,
T1018	2735-2747	RB	denotes	respectively
T1019	2748-2749	-LRB-	denotes	(
T1020	2751-2753	CD	denotes	11
T1021	2749-2750	CD	denotes	3
T1022	2750-2751	,	denotes	,
T1023	2753-2754	-RRB-	denotes	)
T1024	2754-2755	.	denotes	.
T1025	2755-2990	sentence	denotes	Chromatin immunoprecipitation assays and genetic characterization in Saccharomyces cerevisiae strongly suggested that Mcm is involved not only in initiation but also in the DNA chain elongation stage as a replicative helicase (19,20).
T1026	2756-2765	NN	denotes	Chromatin
T1027	2786-2792	NNS	denotes	assays
T1028	2766-2785	NN	denotes	immunoprecipitation
T1029	2859-2868	VBD	denotes	suggested
T1030	2793-2796	CC	denotes	and
T1031	2797-2804	JJ	denotes	genetic
T1032	2805-2821	NN	denotes	characterization
T1033	2822-2824	IN	denotes	in
T1034	2825-2838	NNP	denotes	Saccharomyces
T1035	2839-2849	NNP	denotes	cerevisiae
T1036	2850-2858	RB	denotes	strongly
T1037	2869-2873	IN	denotes	that
T1038	2881-2889	VBN	denotes	involved
T1039	2874-2877	NN	denotes	Mcm
T1040	2878-2880	VBZ	denotes	is
T1041	2890-2893	RB	denotes	not
T1042	2899-2901	IN	denotes	in
T1043	2894-2898	RB	denotes	only
T1044	2902-2912	NN	denotes	initiation
T1045	2913-2916	CC	denotes	but
T1046	2917-2921	RB	denotes	also
T1047	2922-2924	IN	denotes	in
T1048	2925-2928	DT	denotes	the
T1049	2950-2955	NN	denotes	stage
T1050	2929-2932	NN	denotes	DNA
T1051	2939-2949	NN	denotes	elongation
T1052	2933-2938	NN	denotes	chain
T1053	2956-2958	IN	denotes	as
T1054	2959-2960	DT	denotes	a
T1055	2973-2981	NN	denotes	helicase
T1056	2961-2972	JJ	denotes	replicative
T1057	2982-2983	-LRB-	denotes	(
T1058	2986-2988	CD	denotes	20
T1059	2983-2985	CD	denotes	19
T1060	2985-2986	,	denotes	,
T1061	2988-2989	-RRB-	denotes	)
T1062	2989-2990	.	denotes	.
T1063	2990-3203	sentence	denotes	Consistent with this notion, the processivity of the Schizosaccharomyces pombe and mouse Mcm4/6/7 complexes is significantly stimulated on forked DNA structures and it can unwind duplex DNA of 400–500 bp (13,14).
T1064	2991-3001	JJ	denotes	Consistent
T1065	3116-3126	VBN	denotes	stimulated
T1066	3002-3006	IN	denotes	with
T1067	3007-3011	DT	denotes	this
T1068	3012-3018	NN	denotes	notion
T1069	3018-3020	,	denotes	,
T1070	3020-3023	DT	denotes	the
T1071	3024-3036	NN	denotes	processivity
T1072	3037-3039	IN	denotes	of
T1073	3040-3043	DT	denotes	the
T1074	3089-3098	NNS	denotes	complexes
T1075	3044-3063	NNP	denotes	Schizosaccharomyces
T1076	3064-3069	NNP	denotes	pombe
T1077	3070-3073	CC	denotes	and
T1078	3074-3079	NN	denotes	mouse
T1079	3080-3084	NN	denotes	Mcm4
T1080	3084-3085	HYPH	denotes	/
T1081	3085-3086	CD	denotes	6
T1082	3086-3087	HYPH	denotes	/
T1083	3087-3088	CD	denotes	7
T1084	3099-3101	VBZ	denotes	is
T1085	3102-3115	RB	denotes	significantly
T1086	3127-3129	IN	denotes	on
T1087	3130-3136	VBN	denotes	forked
T1088	3137-3140	NN	denotes	DNA
T1089	3141-3151	NNS	denotes	structures
T1090	3152-3155	CC	denotes	and
T1091	3156-3158	PRP	denotes	it
T1092	3163-3169	VB	denotes	unwind
T1093	3159-3162	MD	denotes	can
T1094	3170-3176	NN	denotes	duplex
T1095	3177-3180	NN	denotes	DNA
T1096	3181-3183	IN	denotes	of
T1097	3184-3187	CD	denotes	400
T1098	3188-3191	CD	denotes	500
T1099	3187-3188	SYM	denotes	–
T1100	3192-3194	NN	denotes	bp
T1101	3195-3196	-LRB-	denotes	(
T1102	3199-3201	CD	denotes	14
T1103	3196-3198	CD	denotes	13
T1104	3198-3199	,	denotes	,
T1105	3201-3202	-RRB-	denotes	)
T1106	3202-3203	.	denotes	.
T1107	3203-3357	sentence	denotes	Mcm4/6/7 binds to fork and bubble structures in an ATP-dependent manner, and generates a double-hexameric complex, as was shown for T-antigen (13,14,21).
T1108	3204-3208	NN	denotes	Mcm4
T1109	3213-3218	VBZ	denotes	binds
T1110	3208-3209	HYPH	denotes	/
T1111	3209-3210	CD	denotes	6
T1112	3210-3211	HYPH	denotes	/
T1113	3211-3212	CD	denotes	7
T1114	3219-3221	IN	denotes	to
T1115	3222-3226	NN	denotes	fork
T1116	3238-3248	NNS	denotes	structures
T1117	3227-3230	CC	denotes	and
T1118	3231-3237	NN	denotes	bubble
T1119	3249-3251	IN	denotes	in
T1120	3252-3254	DT	denotes	an
T1121	3269-3275	NN	denotes	manner
T1122	3255-3258	NN	denotes	ATP
T1123	3259-3268	JJ	denotes	dependent
T1124	3258-3259	HYPH	denotes	-
T1125	3275-3277	,	denotes	,
T1126	3277-3280	CC	denotes	and
T1127	3281-3290	VBZ	denotes	generates
T1128	3291-3292	DT	denotes	a
T1129	3310-3317	NN	denotes	complex
T1130	3293-3299	JJ	denotes	double
T1131	3300-3309	JJ	denotes	hexameric
T1132	3299-3300	HYPH	denotes	-
T1133	3317-3319	,	denotes	,
T1134	3319-3321	IN	denotes	as
T1135	3326-3331	VBN	denotes	shown
T1136	3322-3325	VBD	denotes	was
T1137	3332-3335	IN	denotes	for
T1138	3336-3337	NN	denotes	T
T1139	3338-3345	NN	denotes	antigen
T1140	3337-3338	HYPH	denotes	-
T1141	3346-3347	-LRB-	denotes	(
T1142	3353-3355	CD	denotes	21
T1143	3347-3349	CD	denotes	13
T1144	3349-3350	,	denotes	,
T1145	3350-3352	CD	denotes	14
T1146	3352-3353	,	denotes	,
T1147	3355-3356	-RRB-	denotes	)
T1148	3356-3357	.	denotes	.
T1149	3357-3663	sentence	denotes	Recently, we reported that the helicase and ATP hydrolysis activities of mammalian Mcm4/6/7 are specifically activated by single-stranded DNA containing stretches of thymine residues and proposed a novel model that Mcm may play a crucial role in selection of replication origins in higher eukaryotes (13).
T1150	3358-3366	RB	denotes	Recently
T1151	3371-3379	VBD	denotes	reported
T1152	3366-3368	,	denotes	,
T1153	3368-3370	PRP	denotes	we
T1154	3380-3384	IN	denotes	that
T1155	3467-3476	VBN	denotes	activated
T1156	3385-3388	DT	denotes	the
T1157	3417-3427	NNS	denotes	activities
T1158	3389-3397	NN	denotes	helicase
T1159	3398-3401	CC	denotes	and
T1160	3402-3405	NN	denotes	ATP
T1161	3406-3416	NN	denotes	hydrolysis
T1162	3428-3430	IN	denotes	of
T1163	3431-3440	JJ	denotes	mammalian
T1164	3441-3445	NN	denotes	Mcm4
T1165	3445-3446	HYPH	denotes	/
T1166	3446-3447	CD	denotes	6
T1167	3447-3448	HYPH	denotes	/
T1168	3448-3449	CD	denotes	7
T1169	3450-3453	VBP	denotes	are
T1170	3454-3466	RB	denotes	specifically
T1171	3477-3479	IN	denotes	by
T1172	3480-3486	JJ	denotes	single
T1173	3487-3495	VBN	denotes	stranded
T1174	3486-3487	HYPH	denotes	-
T1175	3496-3499	NN	denotes	DNA
T1176	3500-3510	VBG	denotes	containing
T1177	3511-3520	NNS	denotes	stretches
T1178	3521-3523	IN	denotes	of
T1179	3524-3531	NN	denotes	thymine
T1180	3532-3540	NNS	denotes	residues
T1181	3541-3544	CC	denotes	and
T1182	3545-3553	VBD	denotes	proposed
T1183	3554-3555	DT	denotes	a
T1184	3562-3567	NN	denotes	model
T1185	3556-3561	JJ	denotes	novel
T1186	3568-3572	IN	denotes	that
T1187	3581-3585	VB	denotes	play
T1188	3573-3576	NN	denotes	Mcm
T1189	3577-3580	MD	denotes	may
T1190	3586-3587	DT	denotes	a
T1191	3596-3600	NN	denotes	role
T1192	3588-3595	JJ	denotes	crucial
T1193	3601-3603	IN	denotes	in
T1194	3604-3613	NN	denotes	selection
T1195	3614-3616	IN	denotes	of
T1196	3617-3628	NN	denotes	replication
T1197	3629-3636	NNS	denotes	origins
T1198	3637-3639	IN	denotes	in
T1199	3640-3646	JJR	denotes	higher
T1200	3647-3657	NNS	denotes	eukaryotes
T1201	3658-3659	-LRB-	denotes	(
T1202	3659-3661	CD	denotes	13
T1203	3661-3662	-RRB-	denotes	)
T1204	3662-3663	.	denotes	.
T1205	3663-3962	sentence	denotes	In this report, in order to clarify the mode of action of the Mcm helicase and obtain insight into how it may function at the replication forks in vivo, we have conducted detailed analyses of helicase action and DNA binding of mouse Mcm4/6/7 helicase using various forked and bubble substrate DNAs.
T1206	3664-3666	IN	denotes	In
T1207	3825-3834	VBN	denotes	conducted
T1208	3667-3671	DT	denotes	this
T1209	3672-3678	NN	denotes	report
T1210	3678-3680	,	denotes	,
T1211	3680-3682	IN	denotes	in
T1212	3683-3688	NN	denotes	order
T1213	3689-3691	TO	denotes	to
T1214	3692-3699	VB	denotes	clarify
T1215	3700-3703	DT	denotes	the
T1216	3704-3708	NN	denotes	mode
T1217	3709-3711	IN	denotes	of
T1218	3712-3718	NN	denotes	action
T1219	3719-3721	IN	denotes	of
T1220	3722-3725	DT	denotes	the
T1221	3730-3738	NN	denotes	helicase
T1222	3726-3729	NN	denotes	Mcm
T1223	3739-3742	CC	denotes	and
T1224	3743-3749	VB	denotes	obtain
T1225	3750-3757	NN	denotes	insight
T1226	3758-3762	IN	denotes	into
T1227	3763-3766	WRB	denotes	how
T1228	3774-3782	VB	denotes	function
T1229	3767-3769	PRP	denotes	it
T1230	3770-3773	MD	denotes	may
T1231	3783-3785	IN	denotes	at
T1232	3786-3789	DT	denotes	the
T1233	3802-3807	NNS	denotes	forks
T1234	3790-3801	NN	denotes	replication
T1235	3808-3810	FW	denotes	in
T1236	3811-3815	FW	denotes	vivo
T1237	3815-3817	,	denotes	,
T1238	3817-3819	PRP	denotes	we
T1239	3820-3824	VBP	denotes	have
T1240	3835-3843	JJ	denotes	detailed
T1241	3844-3852	NNS	denotes	analyses
T1242	3853-3855	IN	denotes	of
T1243	3856-3864	NN	denotes	helicase
T1244	3865-3871	NN	denotes	action
T1245	3872-3875	CC	denotes	and
T1246	3876-3879	NN	denotes	DNA
T1247	3880-3887	NN	denotes	binding
T1248	3888-3890	IN	denotes	of
T1249	3891-3896	NN	denotes	mouse
T1250	3906-3914	NN	denotes	helicase
T1251	3897-3901	NN	denotes	Mcm4
T1252	3901-3902	HYPH	denotes	/
T1253	3902-3903	CD	denotes	6
T1254	3903-3904	HYPH	denotes	/
T1255	3904-3905	CD	denotes	7
T1256	3915-3920	VBG	denotes	using
T1257	3921-3928	JJ	denotes	various
T1258	3957-3961	NNS	denotes	DNAs
T1259	3929-3935	VBN	denotes	forked
T1260	3936-3939	CC	denotes	and
T1261	3940-3946	NN	denotes	bubble
T1262	3947-3956	NN	denotes	substrate
T1263	3961-3962	.	denotes	.
T1264	3962-4201	sentence	denotes	We specifically addressed sequence requirement for the Mcm helicase activation, mode of interaction with DNA substrates and whether the thymine sequences are required for continuous activation of Mcm helicase during the unwinding process.
T1265	3963-3965	PRP	denotes	We
T1266	3979-3988	VBD	denotes	addressed
T1267	3966-3978	RB	denotes	specifically
T1268	3989-3997	NN	denotes	sequence
T1269	3998-4009	NN	denotes	requirement
T1270	4010-4013	IN	denotes	for
T1271	4014-4017	DT	denotes	the
T1272	4031-4041	NN	denotes	activation
T1273	4018-4021	NN	denotes	Mcm
T1274	4022-4030	NN	denotes	helicase
T1275	4041-4043	,	denotes	,
T1276	4043-4047	NN	denotes	mode
T1277	4048-4050	IN	denotes	of
T1278	4051-4062	NN	denotes	interaction
T1279	4063-4067	IN	denotes	with
T1280	4068-4071	NN	denotes	DNA
T1281	4072-4082	NNS	denotes	substrates
T1282	4083-4086	CC	denotes	and
T1283	4087-4094	IN	denotes	whether
T1284	4121-4129	VBN	denotes	required
T1285	4095-4098	DT	denotes	the
T1286	4107-4116	NNS	denotes	sequences
T1287	4099-4106	NN	denotes	thymine
T1288	4117-4120	VBP	denotes	are
T1289	4130-4133	IN	denotes	for
T1290	4134-4144	JJ	denotes	continuous
T1291	4145-4155	NN	denotes	activation
T1292	4156-4158	IN	denotes	of
T1293	4159-4162	NN	denotes	Mcm
T1294	4163-4171	NN	denotes	helicase
T1295	4172-4178	IN	denotes	during
T1296	4179-4182	DT	denotes	the
T1297	4193-4200	NN	denotes	process
T1298	4183-4192	JJ	denotes	unwinding
T1299	4200-4201	.	denotes	.
T1300	4201-4402	sentence	denotes	The results indicate that mammalian Mcm4/6/7 primarily binds to single-stranded DNA region, and that the extent of helicase activation is related to the thymine content of the single-stranded segment.
T1301	4202-4205	DT	denotes	The
T1302	4206-4213	NNS	denotes	results
T1303	4214-4222	VBP	denotes	indicate
T1304	4223-4227	IN	denotes	that
T1305	4257-4262	VBZ	denotes	binds
T1306	4228-4237	JJ	denotes	mammalian
T1307	4238-4242	NN	denotes	Mcm4
T1308	4242-4243	HYPH	denotes	/
T1309	4243-4244	CD	denotes	6
T1310	4244-4245	HYPH	denotes	/
T1311	4245-4246	CD	denotes	7
T1312	4247-4256	RB	denotes	primarily
T1313	4263-4265	IN	denotes	to
T1314	4266-4272	JJ	denotes	single
T1315	4273-4281	VBN	denotes	stranded
T1316	4272-4273	HYPH	denotes	-
T1317	4286-4292	NN	denotes	region
T1318	4282-4285	NN	denotes	DNA
T1319	4292-4294	,	denotes	,
T1320	4294-4297	CC	denotes	and
T1321	4298-4302	IN	denotes	that
T1322	4340-4347	VBN	denotes	related
T1323	4303-4306	DT	denotes	the
T1324	4307-4313	NN	denotes	extent
T1325	4314-4316	IN	denotes	of
T1326	4317-4325	NN	denotes	helicase
T1327	4326-4336	NN	denotes	activation
T1328	4337-4339	VBZ	denotes	is
T1329	4348-4350	IN	denotes	to
T1330	4351-4354	DT	denotes	the
T1331	4363-4370	NN	denotes	content
T1332	4355-4362	NN	denotes	thymine
T1333	4371-4373	IN	denotes	of
T1334	4374-4377	DT	denotes	the
T1335	4394-4401	NN	denotes	segment
T1336	4378-4384	JJ	denotes	single
T1337	4385-4393	VBN	denotes	stranded
T1338	4384-4385	HYPH	denotes	-
T1339	4401-4402	.	denotes	.
T1340	4402-4607	sentence	denotes	Unexpectedly, Mcm4/6/7 helicase is not capable of efficiently unwinding the GC-rich duplex segment, suggesting that some other mechanism may be required for completion of replication of the entire genome.
T1341	4403-4415	RB	denotes	Unexpectedly
T1342	4435-4437	VBZ	denotes	is
T1343	4415-4417	,	denotes	,
T1344	4417-4421	NN	denotes	Mcm4
T1345	4426-4434	NN	denotes	helicase
T1346	4421-4422	HYPH	denotes	/
T1347	4422-4423	CD	denotes	6
T1348	4423-4424	HYPH	denotes	/
T1349	4424-4425	CD	denotes	7
T1350	4438-4441	RB	denotes	not
T1351	4442-4449	JJ	denotes	capable
T1352	4450-4452	IN	denotes	of
T1353	4453-4464	RB	denotes	efficiently
T1354	4465-4474	VBG	denotes	unwinding
T1355	4475-4478	DT	denotes	the
T1356	4494-4501	NN	denotes	segment
T1357	4479-4481	NN	denotes	GC
T1358	4482-4486	JJ	denotes	rich
T1359	4481-4482	HYPH	denotes	-
T1360	4487-4493	NN	denotes	duplex
T1361	4501-4503	,	denotes	,
T1362	4503-4513	VBG	denotes	suggesting
T1363	4514-4518	IN	denotes	that
T1364	4547-4555	VBN	denotes	required
T1365	4519-4523	DT	denotes	some
T1366	4530-4539	NN	denotes	mechanism
T1367	4524-4529	JJ	denotes	other
T1368	4540-4543	MD	denotes	may
T1369	4544-4546	VB	denotes	be
T1370	4556-4559	IN	denotes	for
T1371	4560-4570	NN	denotes	completion
T1372	4571-4573	IN	denotes	of
T1373	4574-4585	NN	denotes	replication
T1374	4586-4588	IN	denotes	of
T1375	4589-4592	DT	denotes	the
T1376	4600-4606	NN	denotes	genome
T1377	4593-4599	JJ	denotes	entire
T1378	4606-4607	.	denotes	.
T1448	4632-4640	NNS	denotes	Reagents
T1449	4640-4728	sentence	denotes	Labeled and unlabeled dNTPs/rNTPs and Sequenase were purchased from Amersham Pharmacia.
T1450	4641-4648	VBN	denotes	Labeled
T1451	4669-4674	NNS	denotes	rNTPs
T1452	4649-4652	CC	denotes	and
T1453	4653-4662	JJ	denotes	unlabeled
T1454	4663-4668	NNS	denotes	dNTPs
T1455	4668-4669	HYPH	denotes	/
T1456	4694-4703	VBN	denotes	purchased
T1457	4675-4678	CC	denotes	and
T1458	4679-4688	NN	denotes	Sequenase
T1459	4689-4693	VBD	denotes	were
T1460	4704-4708	IN	denotes	from
T1461	4709-4717	NNP	denotes	Amersham
T1462	4718-4727	NNP	denotes	Pharmacia
T1463	4727-4728	.	denotes	.
T1464	4728-4955	sentence	denotes	M13mp18 and M13mp19 single-stranded circular DNA, T4 polynucleotide kinase, DNase I and the Klenow fragment were from TAKARA, Nuclease P1 was from Roche, and anti-FLAG M2 Ab (antibody)-agarose and FLAG peptide were from Sigma.
T1465	4729-4736	NN	denotes	M13mp18
T1466	4774-4777	NN	denotes	DNA
T1467	4737-4740	CC	denotes	and
T1468	4741-4748	NN	denotes	M13mp19
T1469	4749-4755	JJ	denotes	single
T1470	4756-4764	VBN	denotes	stranded
T1471	4755-4756	HYPH	denotes	-
T1472	4765-4773	JJ	denotes	circular
T1473	4837-4841	VBD	denotes	were
T1474	4777-4779	,	denotes	,
T1475	4779-4781	NN	denotes	T4
T1476	4797-4803	NN	denotes	kinase
T1477	4782-4796	NN	denotes	polynucleotide
T1478	4803-4805	,	denotes	,
T1479	4805-4810	NN	denotes	DNase
T1480	4811-4812	CD	denotes	I
T1481	4813-4816	CC	denotes	and
T1482	4817-4820	DT	denotes	the
T1483	4828-4836	NN	denotes	fragment
T1484	4821-4827	NN	denotes	Klenow
T1485	4842-4846	IN	denotes	from
T1486	4847-4853	NN	denotes	TAKARA
T1487	4853-4855	,	denotes	,
T1488	4855-4863	NN	denotes	Nuclease
T1489	4864-4866	NN	denotes	P1
T1490	4867-4870	VBD	denotes	was
T1491	4871-4875	IN	denotes	from
T1492	4876-4881	NNP	denotes	Roche
T1493	4881-4883	,	denotes	,
T1494	4883-4886	CC	denotes	and
T1495	4887-4896	JJ	denotes	anti-FLAG
T1496	4914-4921	NN	denotes	agarose
T1497	4897-4899	NN	denotes	M2
T1498	4900-4902	NN	denotes	Ab
T1499	4904-4912	NN	denotes	antibody
T1500	4903-4904	-LRB-	denotes	(
T1501	4912-4913	-RRB-	denotes	)
T1502	4913-4914	HYPH	denotes	-
T1503	4939-4943	VBD	denotes	were
T1504	4922-4925	CC	denotes	and
T1505	4926-4930	NN	denotes	FLAG
T1506	4931-4938	NN	denotes	peptide
T1507	4944-4948	IN	denotes	from
T1508	4949-4954	NNP	denotes	Sigma
T1509	4954-4955	.	denotes	.
T1510	4955-5040	sentence	denotes	SV40 T-antigen was purified from baculovirus-infected insect cells as reported (22).
T1511	4956-4960	NN	denotes	SV40
T1512	4963-4970	NN	denotes	antigen
T1513	4961-4962	NN	denotes	T
T1514	4962-4963	HYPH	denotes	-
T1515	4975-4983	VBN	denotes	purified
T1516	4971-4974	VBD	denotes	was
T1517	4984-4988	IN	denotes	from
T1518	4989-5000	NN	denotes	baculovirus
T1519	5001-5009	VBN	denotes	infected
T1520	5000-5001	HYPH	denotes	-
T1521	5017-5022	NNS	denotes	cells
T1522	5010-5016	NN	denotes	insect
T1523	5023-5025	IN	denotes	as
T1524	5026-5034	VBN	denotes	reported
T1525	5035-5036	-LRB-	denotes	(
T1526	5036-5038	CD	denotes	22
T1527	5038-5039	-RRB-	denotes	)
T1528	5039-5040	.	denotes	.
T1529	5040-5099	sentence	denotes	PriA helicase protein was provided by Dr Taku Tanaka (23).
T1530	5041-5045	NN	denotes	PriA
T1531	5046-5054	NN	denotes	helicase
T1532	5055-5062	NN	denotes	protein
T1533	5067-5075	VBN	denotes	provided
T1534	5063-5066	VBD	denotes	was
T1535	5076-5078	IN	denotes	by
T1536	5079-5081	NNP	denotes	Dr
T1537	5087-5093	NNP	denotes	Tanaka
T1538	5082-5086	NNP	denotes	Taku
T1539	5094-5095	-LRB-	denotes	(
T1540	5095-5097	CD	denotes	23
T1541	5097-5098	-RRB-	denotes	)
T1542	5098-5099	.	denotes	.
T1543	5099-5301	sentence	denotes	Oligonucleotides were purchased from Hokkaido system science Co., Ltd (Hokkaido, Japan), and were purified on polyacrylamide gel, followed by purification by Sep-Pak Plus C18 cartridge column (Waters).
T1544	5100-5116	NNS	denotes	Oligonucleotides
T1545	5122-5131	VBN	denotes	purchased
T1546	5117-5121	VBD	denotes	were
T1547	5132-5136	IN	denotes	from
T1548	5137-5145	NNP	denotes	Hokkaido
T1549	5166-5169	NNP	denotes	Ltd
T1550	5146-5152	NNP	denotes	system
T1551	5153-5160	NNP	denotes	science
T1552	5161-5164	NNP	denotes	Co.
T1553	5164-5166	,	denotes	,
T1554	5170-5171	-LRB-	denotes	(
T1555	5171-5179	NNP	denotes	Hokkaido
T1556	5179-5181	,	denotes	,
T1557	5181-5186	NNP	denotes	Japan
T1558	5186-5187	-RRB-	denotes	)
T1559	5187-5189	,	denotes	,
T1560	5189-5192	CC	denotes	and
T1561	5193-5197	VBD	denotes	were
T1562	5198-5206	VBN	denotes	purified
T1563	5207-5209	IN	denotes	on
T1564	5210-5224	NN	denotes	polyacrylamide
T1565	5225-5228	NN	denotes	gel
T1566	5228-5230	,	denotes	,
T1567	5230-5238	VBN	denotes	followed
T1568	5239-5241	IN	denotes	by
T1569	5242-5254	NN	denotes	purification
T1570	5255-5257	IN	denotes	by
T1571	5258-5261	NN	denotes	Sep
T1572	5266-5270	NN	denotes	Plus
T1573	5261-5262	HYPH	denotes	-
T1574	5262-5265	NN	denotes	Pak
T1575	5275-5284	NN	denotes	cartridge
T1576	5271-5274	NN	denotes	C18
T1577	5285-5291	NN	denotes	column
T1578	5292-5293	-LRB-	denotes	(
T1579	5293-5299	NNP	denotes	Waters
T1580	5299-5300	-RRB-	denotes	)
T1581	5300-5301	.	denotes	.
T1667	5303-5313	NN	denotes	Expression
T1668	5314-5317	CC	denotes	and
T1669	5318-5330	NN	denotes	purification
T1670	5331-5333	IN	denotes	of
T1671	5334-5339	NN	denotes	mouse
T1672	5349-5356	NN	denotes	complex
T1673	5340-5344	NN	denotes	Mcm4
T1674	5344-5345	HYPH	denotes	/
T1675	5345-5346	CD	denotes	6
T1676	5346-5347	HYPH	denotes	/
T1677	5347-5348	CD	denotes	7
T1678	5356-5466	sentence	denotes	The recombinant baculoviruses expressing His6-Mcm4/Mcm6 or Mcm7-FLAG proteins were previously described (11).
T1679	5357-5360	DT	denotes	The
T1680	5373-5386	NNS	denotes	baculoviruses
T1681	5361-5372	JJ	denotes	recombinant
T1682	5451-5460	VBN	denotes	described
T1683	5387-5397	VBG	denotes	expressing
T1684	5398-5402	NN	denotes	His6
T1685	5408-5412	NN	denotes	Mcm6
T1686	5402-5403	HYPH	denotes	-
T1687	5403-5407	NN	denotes	Mcm4
T1688	5407-5408	HYPH	denotes	/
T1689	5426-5434	NN	denotes	proteins
T1690	5413-5415	CC	denotes	or
T1691	5416-5420	NN	denotes	Mcm7
T1692	5421-5425	NN	denotes	FLAG
T1693	5420-5421	HYPH	denotes	-
T1694	5435-5439	VBD	denotes	were
T1695	5440-5450	RB	denotes	previously
T1696	5461-5462	-LRB-	denotes	(
T1697	5462-5464	CD	denotes	11
T1698	5464-5465	-RRB-	denotes	)
T1699	5465-5466	.	denotes	.
T1700	5466-5618	sentence	denotes	Sf9 and High five insect cells were cultured at 27°C in Sf-900 II SFM (Life Technologies, Inc.) and EX-CELL 405 medium (JRH Biosciences), respectively.
T1701	5467-5470	NN	denotes	Sf9
T1702	5492-5497	NNS	denotes	cells
T1703	5471-5474	CC	denotes	and
T1704	5475-5479	JJ	denotes	High
T1705	5480-5484	CD	denotes	five
T1706	5485-5491	NN	denotes	insect
T1707	5503-5511	VBN	denotes	cultured
T1708	5498-5502	VBD	denotes	were
T1709	5512-5514	IN	denotes	at
T1710	5515-5517	CD	denotes	27
T1711	5517-5519	NN	denotes	°C
T1712	5520-5522	IN	denotes	in
T1713	5523-5525	NN	denotes	Sf
T1714	5533-5536	NN	denotes	SFM
T1715	5525-5526	HYPH	denotes	-
T1716	5526-5529	CD	denotes	900
T1717	5530-5532	CD	denotes	II
T1718	5537-5538	-LRB-	denotes	(
T1719	5557-5561	NNP	denotes	Inc.
T1720	5538-5542	NNP	denotes	Life
T1721	5543-5555	NNP	denotes	Technologies
T1722	5555-5557	,	denotes	,
T1723	5561-5562	-RRB-	denotes	)
T1724	5563-5566	CC	denotes	and
T1725	5567-5569	NN	denotes	EX
T1726	5570-5574	NN	denotes	CELL
T1727	5569-5570	HYPH	denotes	-
T1728	5579-5585	NN	denotes	medium
T1729	5575-5578	CD	denotes	405
T1730	5586-5587	-LRB-	denotes	(
T1731	5591-5602	NNP	denotes	Biosciences
T1732	5587-5590	NNP	denotes	JRH
T1733	5602-5603	-RRB-	denotes	)
T1734	5603-5605	,	denotes	,
T1735	5605-5617	RB	denotes	respectively
T1736	5617-5618	.	denotes	.
T1737	5618-5840	sentence	denotes	For expression of the Mcm4/6/7 proteins, High five cells were coinfected with recombinant baculoviruses expressing the His6-Mcm4/Mcm6 proteins and those expressing the Mcm7-FLAG, and were collected at 48 h post-infection.
T1738	5619-5622	IN	denotes	For
T1739	5681-5691	VBN	denotes	coinfected
T1740	5623-5633	NN	denotes	expression
T1741	5634-5636	IN	denotes	of
T1742	5637-5640	DT	denotes	the
T1743	5650-5658	NN	denotes	proteins
T1744	5641-5645	NN	denotes	Mcm4
T1745	5645-5646	HYPH	denotes	/
T1746	5646-5647	CD	denotes	6
T1747	5647-5648	HYPH	denotes	/
T1748	5648-5649	CD	denotes	7
T1749	5658-5660	,	denotes	,
T1750	5660-5664	JJ	denotes	High
T1751	5665-5669	CD	denotes	five
T1752	5670-5675	NNS	denotes	cells
T1753	5676-5680	VBD	denotes	were
T1754	5692-5696	IN	denotes	with
T1755	5697-5708	JJ	denotes	recombinant
T1756	5709-5722	NNS	denotes	baculoviruses
T1757	5723-5733	VBG	denotes	expressing
T1758	5734-5737	DT	denotes	the
T1759	5753-5761	NN	denotes	proteins
T1760	5738-5742	NN	denotes	His6
T1761	5748-5752	NN	denotes	Mcm6
T1762	5742-5743	HYPH	denotes	-
T1763	5743-5747	NN	denotes	Mcm4
T1764	5747-5748	HYPH	denotes	/
T1765	5762-5765	CC	denotes	and
T1766	5766-5771	DT	denotes	those
T1767	5772-5782	VBG	denotes	expressing
T1768	5783-5786	DT	denotes	the
T1769	5792-5796	NN	denotes	FLAG
T1770	5787-5791	NN	denotes	Mcm7
T1771	5791-5792	HYPH	denotes	-
T1772	5796-5798	,	denotes	,
T1773	5798-5801	CC	denotes	and
T1774	5802-5806	VBD	denotes	were
T1775	5807-5816	VBN	denotes	collected
T1776	5817-5819	IN	denotes	at
T1777	5820-5822	CD	denotes	48
T1778	5823-5824	NN	denotes	h
T1779	5825-5839	RB	denotes	post-infection
T1780	5839-5840	.	denotes	.
T1781	5840-5919	sentence	denotes	The recombinant Mcm4/6/7 complexes were purified as previously described (12).
T1782	5841-5844	DT	denotes	The
T1783	5866-5875	NNS	denotes	complexes
T1784	5845-5856	JJ	denotes	recombinant
T1785	5857-5861	NN	denotes	Mcm4
T1786	5861-5862	HYPH	denotes	/
T1787	5862-5863	CD	denotes	6
T1788	5863-5864	HYPH	denotes	/
T1789	5864-5865	CD	denotes	7
T1790	5881-5889	VBN	denotes	purified
T1791	5876-5880	VBD	denotes	were
T1792	5890-5892	IN	denotes	as
T1793	5904-5913	VBN	denotes	described
T1794	5893-5903	RB	denotes	previously
T1795	5914-5915	-LRB-	denotes	(
T1796	5915-5917	CD	denotes	12
T1797	5917-5918	-RRB-	denotes	)
T1798	5918-5919	.	denotes	.
T1980	5921-5924	NN	denotes	DNA
T1981	5925-5935	NNS	denotes	substrates
T1982	5935-6049	sentence	denotes	The sequences for all the oligonucleotides used for constructions of DNA substrates are listed in Tables 1 and 2.
T1983	5936-5939	DT	denotes	The
T1984	5940-5949	NNS	denotes	sequences
T1985	6024-6030	VBN	denotes	listed
T1986	5950-5953	IN	denotes	for
T1987	5954-5957	PDT	denotes	all
T1988	5962-5978	NNS	denotes	oligonucleotides
T1989	5958-5961	DT	denotes	the
T1990	5979-5983	VBN	denotes	used
T1991	5984-5987	IN	denotes	for
T1992	5988-6001	NNS	denotes	constructions
T1993	6002-6004	IN	denotes	of
T1994	6005-6008	NN	denotes	DNA
T1995	6009-6019	NNS	denotes	substrates
T1996	6020-6023	VBP	denotes	are
T1997	6031-6033	IN	denotes	in
T1998	6034-6040	NNS	denotes	Tables
T1999	6041-6042	CD	denotes	1
T2000	6043-6046	CC	denotes	and
T2001	6047-6048	CD	denotes	2
T2002	6048-6049	.	denotes	.
T2003	6049-6201	sentence	denotes	The partial heteroduplex substrates were constructed by annealing labeled oligonucleotides with M13mp18 single-stranded circular DNA or its derivative.
T2004	6050-6053	DT	denotes	The
T2005	6075-6085	NNS	denotes	substrates
T2006	6054-6061	JJ	denotes	partial
T2007	6062-6074	NN	denotes	heteroduplex
T2008	6091-6102	VBN	denotes	constructed
T2009	6086-6090	VBD	denotes	were
T2010	6103-6105	IN	denotes	by
T2011	6106-6115	VBG	denotes	annealing
T2012	6116-6123	VBN	denotes	labeled
T2013	6124-6140	NNS	denotes	oligonucleotides
T2014	6141-6145	IN	denotes	with
T2015	6146-6153	NN	denotes	M13mp18
T2016	6179-6182	NN	denotes	DNA
T2017	6154-6160	JJ	denotes	single
T2018	6161-6169	VBN	denotes	stranded
T2019	6160-6161	HYPH	denotes	-
T2020	6170-6178	JJ	denotes	circular
T2021	6183-6185	CC	denotes	or
T2022	6186-6189	PRP$	denotes	its
T2023	6190-6200	NN	denotes	derivative
T2024	6200-6201	.	denotes	.
T2025	6201-6308	sentence	denotes	The labeled substrates were purified by Sepharose CL4B column chromatography (Amersham Pharmacia Biotech).
T2026	6202-6205	DT	denotes	The
T2027	6214-6224	NNS	denotes	substrates
T2028	6206-6213	VBN	denotes	labeled
T2029	6230-6238	VBN	denotes	purified
T2030	6225-6229	VBD	denotes	were
T2031	6239-6241	IN	denotes	by
T2032	6242-6251	NN	denotes	Sepharose
T2033	6252-6256	NN	denotes	CL4B
T2034	6264-6278	NN	denotes	chromatography
T2035	6257-6263	NN	denotes	column
T2036	6279-6280	-LRB-	denotes	(
T2037	6299-6306	NNP	denotes	Biotech
T2038	6280-6288	NNP	denotes	Amersham
T2039	6289-6298	NNP	denotes	Pharmacia
T2040	6306-6307	-RRB-	denotes	)
T2041	6307-6308	.	denotes	.
T2042	6308-6447	sentence	denotes	The DNA bubble substrates were assembled from two partially complementary oligonucleotides with top and bottom strand sequences (Table 1).
T2043	6309-6312	DT	denotes	The
T2044	6324-6334	NNS	denotes	substrates
T2045	6313-6316	NN	denotes	DNA
T2046	6317-6323	NN	denotes	bubble
T2047	6340-6349	VBN	denotes	assembled
T2048	6335-6339	VBD	denotes	were
T2049	6350-6354	IN	denotes	from
T2050	6355-6358	CD	denotes	two
T2051	6383-6399	NNS	denotes	oligonucleotides
T2052	6359-6368	RB	denotes	partially
T2053	6369-6382	JJ	denotes	complementary
T2054	6400-6404	IN	denotes	with
T2055	6405-6408	JJ	denotes	top
T2056	6427-6436	NNS	denotes	sequences
T2057	6409-6412	CC	denotes	and
T2058	6413-6419	JJ	denotes	bottom
T2059	6420-6426	NN	denotes	strand
T2060	6437-6438	-LRB-	denotes	(
T2061	6438-6443	NN	denotes	Table
T2062	6444-6445	CD	denotes	1
T2063	6445-6446	-RRB-	denotes	)
T2064	6446-6447	.	denotes	.
T2065	6447-6546	sentence	denotes	T-tailed Y-fork substrates were prepared by annealing two oligonucleotides (dT30 and dT60 series).
T2066	6448-6449	NN	denotes	T
T2067	6450-6456	VBN	denotes	tailed
T2068	6449-6450	HYPH	denotes	-
T2069	6464-6474	NNS	denotes	substrates
T2070	6457-6458	NN	denotes	Y
T2071	6459-6463	NN	denotes	fork
T2072	6458-6459	HYPH	denotes	-
T2073	6480-6488	VBN	denotes	prepared
T2074	6475-6479	VBD	denotes	were
T2075	6489-6491	IN	denotes	by
T2076	6492-6501	VBG	denotes	annealing
T2077	6502-6505	CD	denotes	two
T2078	6506-6522	NNS	denotes	oligonucleotides
T2079	6523-6524	-LRB-	denotes	(
T2080	6538-6544	NNS	denotes	series
T2081	6524-6528	NN	denotes	dT30
T2082	6529-6532	CC	denotes	and
T2083	6533-6537	NN	denotes	dT60
T2084	6544-6545	-RRB-	denotes	)
T2085	6545-6546	.	denotes	.
T2086	6546-6686	sentence	denotes	To construct the arrested fork and 3′- or 5′-extension substrates, various combinations of oligonucleotides shown in Table 1 were annealed.
T2087	6547-6549	TO	denotes	To
T2088	6550-6559	VB	denotes	construct
T2089	6677-6685	VBN	denotes	annealed
T2090	6560-6563	DT	denotes	the
T2091	6573-6577	NN	denotes	fork
T2092	6564-6572	VBN	denotes	arrested
T2093	6578-6581	CC	denotes	and
T2094	6582-6583	CD	denotes	3
T2095	6602-6612	NNS	denotes	substrates
T2096	6583-6584	SYM	denotes	′
T2097	6584-6585	HYPH	denotes	-
T2098	6586-6588	CC	denotes	or
T2099	6589-6590	CD	denotes	5
T2100	6590-6591	SYM	denotes	′
T2101	6591-6592	HYPH	denotes	-
T2102	6592-6601	NN	denotes	extension
T2103	6612-6614	,	denotes	,
T2104	6614-6621	JJ	denotes	various
T2105	6622-6634	NNS	denotes	combinations
T2106	6635-6637	IN	denotes	of
T2107	6638-6654	NNS	denotes	oligonucleotides
T2108	6655-6660	VBN	denotes	shown
T2109	6661-6663	IN	denotes	in
T2110	6664-6669	NN	denotes	Table
T2111	6670-6671	CD	denotes	1
T2112	6672-6676	VBD	denotes	were
T2113	6685-6686	.	denotes	.
T2114	6686-6807	sentence	denotes	Bub-82/lamin B2 containing the T-rich strand of the lamin B2 origin region was constructed as previously described (13).
T2115	6687-6690	NN	denotes	Bub
T2116	6700-6702	NN	denotes	B2
T2117	6690-6691	HYPH	denotes	-
T2118	6691-6693	CD	denotes	82
T2119	6693-6694	HYPH	denotes	/
T2120	6694-6699	NN	denotes	lamin
T2121	6766-6777	VBN	denotes	constructed
T2122	6703-6713	VBG	denotes	containing
T2123	6714-6717	DT	denotes	the
T2124	6725-6731	NN	denotes	strand
T2125	6718-6719	NN	denotes	T
T2126	6720-6724	JJ	denotes	rich
T2127	6719-6720	HYPH	denotes	-
T2128	6732-6734	IN	denotes	of
T2129	6735-6738	DT	denotes	the
T2130	6755-6761	NN	denotes	region
T2131	6739-6744	NN	denotes	lamin
T2132	6745-6747	NN	denotes	B2
T2133	6748-6754	NN	denotes	origin
T2134	6762-6765	VBD	denotes	was
T2135	6778-6780	IN	denotes	as
T2136	6792-6801	VBN	denotes	described
T2137	6781-6791	RB	denotes	previously
T2138	6802-6803	-LRB-	denotes	(
T2139	6803-6805	CD	denotes	13
T2140	6805-6806	-RRB-	denotes	)
T2141	6806-6807	.	denotes	.
T2142	6807-7029	sentence	denotes	The bubble substrate, c-myc/DUE or c-myc/DUE-C, contains sequences (GenBank accession number K01908) from nucleotides 722 to 853 or those from 828 to 747 (complementary strand), respectively, in the central melted region.
T2143	6808-6811	DT	denotes	The
T2144	6819-6828	NN	denotes	substrate
T2145	6812-6818	NN	denotes	bubble
T2146	6856-6864	VBZ	denotes	contains
T2147	6828-6830	,	denotes	,
T2148	6830-6831	NN	denotes	c
T2149	6832-6835	NN	denotes	myc
T2150	6831-6832	HYPH	denotes	-
T2151	6836-6839	NN	denotes	DUE
T2152	6835-6836	HYPH	denotes	/
T2153	6840-6842	CC	denotes	or
T2154	6843-6844	NN	denotes	c
T2155	6845-6848	NN	denotes	myc
T2156	6844-6845	HYPH	denotes	-
T2157	6853-6854	NN	denotes	C
T2158	6848-6849	HYPH	denotes	/
T2159	6849-6852	NN	denotes	DUE
T2160	6852-6853	HYPH	denotes	-
T2161	6854-6856	,	denotes	,
T2162	6865-6874	NNS	denotes	sequences
T2163	6875-6876	-LRB-	denotes	(
T2164	6901-6907	NN	denotes	K01908
T2165	6876-6883	NNP	denotes	GenBank
T2166	6894-6900	NN	denotes	number
T2167	6884-6893	NN	denotes	accession
T2168	6907-6908	-RRB-	denotes	)
T2169	6909-6913	IN	denotes	from
T2170	6914-6925	NNS	denotes	nucleotides
T2171	6933-6936	CD	denotes	853
T2172	6926-6929	CD	denotes	722
T2173	6930-6932	IN	denotes	to
T2174	6937-6939	CC	denotes	or
T2175	6940-6945	DT	denotes	those
T2176	6946-6950	IN	denotes	from
T2177	6951-6954	CD	denotes	828
T2178	6955-6957	IN	denotes	to
T2179	6958-6961	CD	denotes	747
T2180	6962-6963	-LRB-	denotes	(
T2181	6977-6983	NN	denotes	strand
T2182	6963-6976	JJ	denotes	complementary
T2183	6983-6984	-RRB-	denotes	)
T2184	6984-6986	,	denotes	,
T2185	6986-6998	RB	denotes	respectively
T2186	6998-7000	,	denotes	,
T2187	7000-7002	IN	denotes	in
T2188	7003-7006	DT	denotes	the
T2189	7022-7028	NN	denotes	region
T2190	7007-7014	JJ	denotes	central
T2191	7015-7021	VBN	denotes	melted
T2192	7028-7029	.	denotes	.
T2193	7029-7328	sentence	denotes	For each substrate, labeled oligonucleotide (3 pmol) was annealed with the unlabeled oligonucleotide (6 pmol) in a reaction mixture (50 μl) containing 20 mM Tris–HCl (pH 7.5), 10 mM MgCl2 and 25 mM NaCl, which were heated to 95°C, kept at 67°C for 1 h, and then allowed to slowly cool down to 37°C.
T2194	7030-7033	IN	denotes	For
T2195	7087-7095	VBN	denotes	annealed
T2196	7034-7038	DT	denotes	each
T2197	7039-7048	NN	denotes	substrate
T2198	7048-7050	,	denotes	,
T2199	7050-7057	VBN	denotes	labeled
T2200	7058-7073	NN	denotes	oligonucleotide
T2201	7074-7075	-LRB-	denotes	(
T2202	7077-7081	NN	denotes	pmol
T2203	7075-7076	CD	denotes	3
T2204	7081-7082	-RRB-	denotes	)
T2205	7083-7086	VBD	denotes	was
T2206	7096-7100	IN	denotes	with
T2207	7101-7104	DT	denotes	the
T2208	7115-7130	NN	denotes	oligonucleotide
T2209	7105-7114	JJ	denotes	unlabeled
T2210	7131-7132	-LRB-	denotes	(
T2211	7134-7138	NN	denotes	pmol
T2212	7132-7133	CD	denotes	6
T2213	7138-7139	-RRB-	denotes	)
T2214	7140-7142	IN	denotes	in
T2215	7143-7144	DT	denotes	a
T2216	7154-7161	NN	denotes	mixture
T2217	7145-7153	NN	denotes	reaction
T2218	7162-7163	-LRB-	denotes	(
T2219	7166-7168	NN	denotes	μl
T2220	7163-7165	CD	denotes	50
T2221	7168-7169	-RRB-	denotes	)
T2222	7170-7180	VBG	denotes	containing
T2223	7181-7183	CD	denotes	20
T2224	7184-7186	NN	denotes	mM
T2225	7192-7195	NN	denotes	HCl
T2226	7187-7191	NN	denotes	Tris
T2227	7191-7192	HYPH	denotes	–
T2228	7196-7197	-LRB-	denotes	(
T2229	7197-7199	NN	denotes	pH
T2230	7200-7203	CD	denotes	7.5
T2231	7203-7204	-RRB-	denotes	)
T2232	7204-7206	,	denotes	,
T2233	7206-7208	CD	denotes	10
T2234	7209-7211	NN	denotes	mM
T2235	7212-7217	NN	denotes	MgCl2
T2236	7218-7221	CC	denotes	and
T2237	7222-7224	CD	denotes	25
T2238	7225-7227	NN	denotes	mM
T2239	7228-7232	NN	denotes	NaCl
T2240	7232-7234	,	denotes	,
T2241	7234-7239	WDT	denotes	which
T2242	7245-7251	VBN	denotes	heated
T2243	7240-7244	VBD	denotes	were
T2244	7252-7254	IN	denotes	to
T2245	7255-7257	CD	denotes	95
T2246	7257-7259	NN	denotes	°C
T2247	7259-7261	,	denotes	,
T2248	7261-7265	VBD	denotes	kept
T2249	7266-7268	IN	denotes	at
T2250	7269-7271	CD	denotes	67
T2251	7271-7273	NN	denotes	°C
T2252	7274-7277	IN	denotes	for
T2253	7278-7279	CD	denotes	1
T2254	7280-7281	NN	denotes	h
T2255	7281-7283	,	denotes	,
T2256	7283-7286	CC	denotes	and
T2257	7287-7291	RB	denotes	then
T2258	7292-7299	VBN	denotes	allowed
T2259	7300-7302	TO	denotes	to
T2260	7310-7314	VB	denotes	cool
T2261	7303-7309	RB	denotes	slowly
T2262	7315-7319	RP	denotes	down
T2263	7320-7322	IN	denotes	to
T2264	7323-7325	CD	denotes	37
T2265	7325-7327	NN	denotes	°C
T2266	7327-7328	.	denotes	.
T2267	7328-7423	sentence	denotes	The assembled substrates were purified from polyacrylamide gel by elution into TE buffer (24).
T2268	7329-7332	DT	denotes	The
T2269	7343-7353	NNS	denotes	substrates
T2270	7333-7342	VBN	denotes	assembled
T2271	7359-7367	VBN	denotes	purified
T2272	7354-7358	VBD	denotes	were
T2273	7368-7372	IN	denotes	from
T2274	7373-7387	NN	denotes	polyacrylamide
T2275	7388-7391	NN	denotes	gel
T2276	7392-7394	IN	denotes	by
T2277	7395-7402	NN	denotes	elution
T2278	7403-7407	IN	denotes	into
T2279	7408-7410	NN	denotes	TE
T2280	7411-7417	NN	denotes	buffer
T2281	7418-7419	-LRB-	denotes	(
T2282	7419-7421	CD	denotes	24
T2283	7421-7422	-RRB-	denotes	)
T2284	7422-7423	.	denotes	.
T2285	7423-7717	sentence	denotes	The partial heteroduplex substrates on a single-stranded circular DNA with varied lengths of duplex regions were prepared by extending DNA chains from the 3′ end of the dT40-37mer (37mer region hybridizing at positions 6289–6326 of M13mp18 vector) annealed to the single-stranded circular DNA.
T2286	7424-7427	DT	denotes	The
T2287	7449-7459	NNS	denotes	substrates
T2288	7428-7435	JJ	denotes	partial
T2289	7436-7448	NN	denotes	heteroduplex
T2290	7537-7545	VBN	denotes	prepared
T2291	7460-7462	IN	denotes	on
T2292	7463-7464	DT	denotes	a
T2293	7490-7493	NN	denotes	DNA
T2294	7465-7471	JJ	denotes	single
T2295	7472-7480	VBN	denotes	stranded
T2296	7471-7472	HYPH	denotes	-
T2297	7481-7489	JJ	denotes	circular
T2298	7494-7498	IN	denotes	with
T2299	7499-7505	VBN	denotes	varied
T2300	7506-7513	NNS	denotes	lengths
T2301	7514-7516	IN	denotes	of
T2302	7517-7523	NN	denotes	duplex
T2303	7524-7531	NNS	denotes	regions
T2304	7532-7536	VBD	denotes	were
T2305	7546-7548	IN	denotes	by
T2306	7549-7558	VBG	denotes	extending
T2307	7559-7562	NN	denotes	DNA
T2308	7563-7569	NNS	denotes	chains
T2309	7570-7574	IN	denotes	from
T2310	7575-7578	DT	denotes	the
T2311	7582-7585	NN	denotes	end
T2312	7579-7580	CD	denotes	3
T2313	7580-7581	SYM	denotes	′
T2314	7586-7588	IN	denotes	of
T2315	7589-7592	DT	denotes	the
T2316	7598-7603	NN	denotes	37mer
T2317	7593-7597	NN	denotes	dT40
T2318	7597-7598	HYPH	denotes	-
T2319	7604-7605	-LRB-	denotes	(
T2320	7605-7610	NN	denotes	37mer
T2321	7611-7617	NN	denotes	region
T2322	7618-7629	VBG	denotes	hybridizing
T2323	7630-7632	IN	denotes	at
T2324	7633-7642	NNS	denotes	positions
T2325	7643-7647	CD	denotes	6289
T2326	7647-7648	SYM	denotes	–
T2327	7648-7652	CD	denotes	6326
T2328	7653-7655	IN	denotes	of
T2329	7656-7663	NN	denotes	M13mp18
T2330	7664-7670	NN	denotes	vector
T2331	7670-7671	-RRB-	denotes	)
T2332	7672-7680	VBN	denotes	annealed
T2333	7681-7683	IN	denotes	to
T2334	7684-7687	DT	denotes	the
T2335	7713-7716	NN	denotes	DNA
T2336	7688-7694	JJ	denotes	single
T2337	7695-7703	VBN	denotes	stranded
T2338	7694-7695	HYPH	denotes	-
T2339	7704-7712	JJ	denotes	circular
T2340	7716-7717	.	denotes	.
T2341	7717-7938	sentence	denotes	DNA chains were elongated with Sequenase in the presence of [α-32P]dGTP, followed by extension after addition of ddGTP and all four dNTPs, resulting in substrates containing labeled duplex regions of varied lengths (13).
T2342	7718-7721	NN	denotes	DNA
T2343	7722-7728	NNS	denotes	chains
T2344	7734-7743	VBN	denotes	elongated
T2345	7729-7733	VBD	denotes	were
T2346	7744-7748	IN	denotes	with
T2347	7749-7758	NN	denotes	Sequenase
T2348	7759-7761	IN	denotes	in
T2349	7762-7765	DT	denotes	the
T2350	7766-7774	NN	denotes	presence
T2351	7775-7777	IN	denotes	of
T2352	7778-7779	-LRB-	denotes	[
T2353	7785-7789	NN	denotes	dGTP
T2354	7779-7780	NN	denotes	α
T2355	7781-7784	NN	denotes	32P
T2356	7780-7781	HYPH	denotes	-
T2357	7784-7785	-RRB-	denotes	]
T2358	7789-7791	,	denotes	,
T2359	7791-7799	VBN	denotes	followed
T2360	7800-7802	IN	denotes	by
T2361	7803-7812	NN	denotes	extension
T2362	7813-7818	IN	denotes	after
T2363	7819-7827	NN	denotes	addition
T2364	7828-7830	IN	denotes	of
T2365	7831-7836	NN	denotes	ddGTP
T2366	7837-7840	CC	denotes	and
T2367	7841-7844	DT	denotes	all
T2368	7850-7855	NNS	denotes	dNTPs
T2369	7845-7849	CD	denotes	four
T2370	7855-7857	,	denotes	,
T2371	7857-7866	VBG	denotes	resulting
T2372	7867-7869	IN	denotes	in
T2373	7870-7880	NNS	denotes	substrates
T2374	7881-7891	VBG	denotes	containing
T2375	7892-7899	VBN	denotes	labeled
T2376	7907-7914	NNS	denotes	regions
T2377	7900-7906	NN	denotes	duplex
T2378	7915-7917	IN	denotes	of
T2379	7918-7924	VBN	denotes	varied
T2380	7925-7932	NNS	denotes	lengths
T2381	7933-7934	-LRB-	denotes	(
T2382	7934-7936	CD	denotes	13
T2383	7936-7937	-RRB-	denotes	)
T2384	7937-7938	.	denotes	.
T2385	7938-8212	sentence	denotes	The substrate (5 fmol) was first incubated with indicated amount of the Mcm4/6/7 complex at 37°C for 1 h in reaction mixture as described (11), and then reactions were stopped by addition of EDTA (20 mM), SDS (0.1%) and 2 μg proteinase K, followed by incubation for 20 min.
T2386	7939-7942	DT	denotes	The
T2387	7943-7952	NN	denotes	substrate
T2388	7972-7981	VBN	denotes	incubated
T2389	7953-7954	-LRB-	denotes	(
T2390	7956-7960	NN	denotes	fmol
T2391	7954-7955	CD	denotes	5
T2392	7960-7961	-RRB-	denotes	)
T2393	7962-7965	VBD	denotes	was
T2394	7966-7971	RB	denotes	first
T2395	7982-7986	IN	denotes	with
T2396	7987-7996	VBN	denotes	indicated
T2397	7997-8003	NN	denotes	amount
T2398	8004-8006	IN	denotes	of
T2399	8007-8010	DT	denotes	the
T2400	8020-8027	NN	denotes	complex
T2401	8011-8015	NN	denotes	Mcm4
T2402	8015-8016	HYPH	denotes	/
T2403	8016-8017	CD	denotes	6
T2404	8017-8018	HYPH	denotes	/
T2405	8018-8019	CD	denotes	7
T2406	8028-8030	IN	denotes	at
T2407	8031-8033	CD	denotes	37
T2408	8033-8035	NN	denotes	°C
T2409	8036-8039	IN	denotes	for
T2410	8040-8041	CD	denotes	1
T2411	8042-8043	NN	denotes	h
T2412	8044-8046	IN	denotes	in
T2413	8047-8055	NN	denotes	reaction
T2414	8056-8063	NN	denotes	mixture
T2415	8064-8066	IN	denotes	as
T2416	8067-8076	VBN	denotes	described
T2417	8077-8078	-LRB-	denotes	(
T2418	8078-8080	CD	denotes	11
T2419	8080-8081	-RRB-	denotes	)
T2420	8081-8083	,	denotes	,
T2421	8083-8086	CC	denotes	and
T2422	8087-8091	RB	denotes	then
T2423	8092-8101	NNS	denotes	reactions
T2424	8107-8114	VBN	denotes	stopped
T2425	8102-8106	VBD	denotes	were
T2426	8115-8117	IN	denotes	by
T2427	8118-8126	NN	denotes	addition
T2428	8127-8129	IN	denotes	of
T2429	8130-8134	NN	denotes	EDTA
T2430	8135-8136	-LRB-	denotes	(
T2431	8139-8141	NN	denotes	mM
T2432	8136-8138	CD	denotes	20
T2433	8141-8142	-RRB-	denotes	)
T2434	8142-8144	,	denotes	,
T2435	8144-8147	NN	denotes	SDS
T2436	8148-8149	-LRB-	denotes	(
T2437	8152-8153	NN	denotes	%
T2438	8149-8152	CD	denotes	0.1
T2439	8153-8154	-RRB-	denotes	)
T2440	8155-8158	CC	denotes	and
T2441	8159-8160	CD	denotes	2
T2442	8161-8163	NN	denotes	μg
T2443	8175-8176	NN	denotes	K
T2444	8164-8174	NN	denotes	proteinase
T2445	8176-8178	,	denotes	,
T2446	8178-8186	VBN	denotes	followed
T2447	8187-8189	IN	denotes	by
T2448	8190-8200	NN	denotes	incubation
T2449	8201-8204	IN	denotes	for
T2450	8205-8207	CD	denotes	20
T2451	8208-8211	NN	denotes	min
T2452	8211-8212	.	denotes	.
T2453	8212-8301	sentence	denotes	Aliquots were electrophoresed on a 6.5% polyacrylamide gel in 1× TBE at 300 V for 2.5 h.
T2454	8213-8221	NNS	denotes	Aliquots
T2455	8227-8242	VBN	denotes	electrophoresed
T2456	8222-8226	VBD	denotes	were
T2457	8243-8245	IN	denotes	on
T2458	8246-8247	DT	denotes	a
T2459	8268-8271	NN	denotes	gel
T2460	8248-8251	CD	denotes	6.5
T2461	8251-8252	NN	denotes	%
T2462	8253-8267	NN	denotes	polyacrylamide
T2463	8272-8274	IN	denotes	in
T2464	8275-8276	CD	denotes	1
T2465	8278-8281	NN	denotes	TBE
T2466	8276-8277	SYM	denotes	×
T2467	8282-8284	IN	denotes	at
T2468	8285-8288	CD	denotes	300
T2469	8289-8290	NN	denotes	V
T2470	8291-8294	IN	denotes	for
T2471	8295-8298	CD	denotes	2.5
T2472	8299-8300	NN	denotes	h
T2473	8300-8301	.	denotes	.
T2474	8301-8373	sentence	denotes	The single-stranded circular DNAs used were M13mp18 and M13mp19+G-rich.
T2475	8302-8305	DT	denotes	The
T2476	8331-8335	NNS	denotes	DNAs
T2477	8306-8312	JJ	denotes	single
T2478	8313-8321	VBN	denotes	stranded
T2479	8312-8313	HYPH	denotes	-
T2480	8322-8330	JJ	denotes	circular
T2481	8341-8345	VBD	denotes	were
T2482	8336-8340	VBN	denotes	used
T2483	8346-8353	NN	denotes	M13mp18
T2484	8354-8357	CC	denotes	and
T2485	8358-8365	NN	denotes	M13mp19
T2486	8365-8366	SYM	denotes	+
T2487	8366-8367	NN	denotes	G
T2488	8367-8368	HYPH	denotes	-
T2489	8368-8372	JJ	denotes	rich
T2490	8372-8373	.	denotes	.
T2491	8373-8633	sentence	denotes	The latter was constructed by inserting a G-rich repeat sequence (GGGGCGTGGGC)6, prepared by annealing of two oligonucleotides (5′-GATCC-[GGGGCGTGGGC]6-3′/5′-GATCC-[GCCCACGCCCC]6-3′) at the BamHI site of M13mp19 and the sequences of the insert were confirmed.
T2492	8374-8377	DT	denotes	The
T2493	8378-8384	JJ	denotes	latter
T2494	8389-8400	VBN	denotes	constructed
T2495	8385-8388	VBD	denotes	was
T2496	8401-8403	IN	denotes	by
T2497	8404-8413	VBG	denotes	inserting
T2498	8414-8415	DT	denotes	a
T2499	8430-8438	NN	denotes	sequence
T2500	8416-8417	NN	denotes	G
T2501	8417-8418	HYPH	denotes	-
T2502	8418-8422	JJ	denotes	rich
T2503	8423-8429	NN	denotes	repeat
T2504	8439-8440	-LRB-	denotes	(
T2505	8440-8451	NN	denotes	GGGGCGTGGGC
T2506	8451-8452	-RRB-	denotes	)
T2507	8452-8453	CD	denotes	6
T2508	8453-8455	,	denotes	,
T2509	8455-8463	VBN	denotes	prepared
T2510	8464-8466	IN	denotes	by
T2511	8467-8476	VBG	denotes	annealing
T2512	8477-8479	IN	denotes	of
T2513	8480-8483	CD	denotes	two
T2514	8484-8500	NNS	denotes	oligonucleotides
T2515	8501-8502	-LRB-	denotes	(
T2516	8502-8503	CD	denotes	5
T2517	8503-8504	SYM	denotes	′
T2518	8504-8505	HYPH	denotes	-
T2519	8505-8510	NN	denotes	GATCC
T2520	8512-8523	NN	denotes	GGGGCGTGGGC
T2521	8510-8511	HYPH	denotes	-
T2522	8511-8512	-LRB-	denotes	[
T2523	8523-8524	-RRB-	denotes	]
T2524	8524-8525	CD	denotes	6
T2525	8525-8526	HYPH	denotes	-
T2526	8526-8527	CD	denotes	3
T2527	8527-8528	SYM	denotes	′
T2528	8528-8529	HYPH	denotes	/
T2529	8529-8530	CD	denotes	5
T2530	8530-8531	SYM	denotes	′
T2531	8531-8532	HYPH	denotes	-
T2532	8532-8537	NN	denotes	GATCC
T2533	8539-8550	NN	denotes	GCCCACGCCCC
T2534	8537-8538	HYPH	denotes	-
T2535	8538-8539	-LRB-	denotes	[
T2536	8550-8551	-RRB-	denotes	]
T2537	8551-8552	CD	denotes	6
T2538	8552-8553	HYPH	denotes	-
T2539	8553-8554	CD	denotes	3
T2540	8554-8555	SYM	denotes	′
T2541	8555-8556	-RRB-	denotes	)
T2542	8557-8559	IN	denotes	at
T2543	8560-8563	DT	denotes	the
T2544	8570-8574	NN	denotes	site
T2545	8564-8569	NN	denotes	BamHI
T2546	8575-8577	IN	denotes	of
T2547	8578-8585	NN	denotes	M13mp19
T2548	8586-8589	CC	denotes	and
T2549	8590-8593	DT	denotes	the
T2550	8594-8603	NNS	denotes	sequences
T2551	8623-8632	VBN	denotes	confirmed
T2552	8604-8606	IN	denotes	of
T2553	8607-8610	DT	denotes	the
T2554	8611-8617	NN	denotes	insert
T2555	8618-8622	VBD	denotes	were
T2556	8632-8633	.	denotes	.
T2629	8635-8638	NN	denotes	DNA
T2630	8639-8647	NN	denotes	helicase
T2631	8662-8668	NNS	denotes	assays
T2632	8648-8651	CC	denotes	and
T2633	8652-8655	NN	denotes	gel
T2634	8656-8661	NN	denotes	shift
T2635	8668-8938	sentence	denotes	In gel shift assays, Mcm4/6/7 proteins were incubated at 30°C for 30 min in reaction mixtures (15 μl) containing 25 mM HEPES–NaOH (pH 7.5), 50 mM sodium acetate, 10 mM Mg(CH3COO)2, 1 mM DTT, 0.25 μg/ml BSA, 0.5 mM ATP-γ-S and labeled substrates of the amount indicated.
T2636	8669-8671	IN	denotes	In
T2637	8713-8722	VBN	denotes	incubated
T2638	8672-8675	NN	denotes	gel
T2639	8676-8681	NN	denotes	shift
T2640	8682-8688	NNS	denotes	assays
T2641	8688-8690	,	denotes	,
T2642	8690-8694	NN	denotes	Mcm4
T2643	8699-8707	NN	denotes	proteins
T2644	8694-8695	HYPH	denotes	/
T2645	8695-8696	CD	denotes	6
T2646	8696-8697	HYPH	denotes	/
T2647	8697-8698	CD	denotes	7
T2648	8708-8712	VBD	denotes	were
T2649	8723-8725	IN	denotes	at
T2650	8726-8728	CD	denotes	30
T2651	8728-8730	NN	denotes	°C
T2652	8731-8734	IN	denotes	for
T2653	8735-8737	CD	denotes	30
T2654	8738-8741	NN	denotes	min
T2655	8742-8744	IN	denotes	in
T2656	8745-8753	NN	denotes	reaction
T2657	8754-8762	NNS	denotes	mixtures
T2658	8763-8764	-LRB-	denotes	(
T2659	8767-8769	NN	denotes	μl
T2660	8764-8766	CD	denotes	15
T2661	8769-8770	-RRB-	denotes	)
T2662	8771-8781	VBG	denotes	containing
T2663	8782-8784	CD	denotes	25
T2664	8785-8787	NN	denotes	mM
T2665	8794-8798	NN	denotes	NaOH
T2666	8788-8793	NN	denotes	HEPES
T2667	8793-8794	HYPH	denotes	–
T2668	8799-8800	-LRB-	denotes	(
T2669	8800-8802	NN	denotes	pH
T2670	8803-8806	CD	denotes	7.5
T2671	8806-8807	-RRB-	denotes	)
T2672	8807-8809	,	denotes	,
T2673	8809-8811	CD	denotes	50
T2674	8812-8814	NN	denotes	mM
T2675	8822-8829	NN	denotes	acetate
T2676	8815-8821	NN	denotes	sodium
T2677	8829-8831	,	denotes	,
T2678	8831-8833	CD	denotes	10
T2679	8834-8836	NN	denotes	mM
T2680	8840-8846	NN	denotes	CH3COO
T2681	8837-8839	NN	denotes	Mg
T2682	8839-8840	-LRB-	denotes	(
T2683	8846-8847	-RRB-	denotes	)
T2684	8847-8848	CD	denotes	2
T2685	8848-8850	,	denotes	,
T2686	8850-8851	CD	denotes	1
T2687	8852-8854	NN	denotes	mM
T2688	8855-8858	NN	denotes	DTT
T2689	8858-8860	,	denotes	,
T2690	8860-8864	CD	denotes	0.25
T2691	8865-8867	NN	denotes	μg
T2692	8871-8874	NN	denotes	BSA
T2693	8867-8868	SYM	denotes	/
T2694	8868-8870	NN	denotes	ml
T2695	8874-8876	,	denotes	,
T2696	8876-8879	CD	denotes	0.5
T2697	8880-8882	NN	denotes	mM
T2698	8889-8890	NN	denotes	S
T2699	8883-8886	NN	denotes	ATP
T2700	8886-8887	HYPH	denotes	-
T2701	8887-8888	SYM	denotes	γ
T2702	8888-8889	HYPH	denotes	-
T2703	8891-8894	CC	denotes	and
T2704	8895-8902	VBN	denotes	labeled
T2705	8903-8913	NNS	denotes	substrates
T2706	8914-8916	IN	denotes	of
T2707	8917-8920	DT	denotes	the
T2708	8921-8927	NN	denotes	amount
T2709	8928-8937	VBN	denotes	indicated
T2710	8937-8938	.	denotes	.
T2711	8938-9164	sentence	denotes	After addition of 2 μl of 50% glycerol, the reaction mixtures were directly applied on a polyacrylamide gel containing 6 mM magnesium acetate and 5% glycerol in 0.5× TBE, and electrophoresis was conducted at room temperature.
T2712	8939-8944	IN	denotes	After
T2713	9015-9022	VBN	denotes	applied
T2714	8945-8953	NN	denotes	addition
T2715	8954-8956	IN	denotes	of
T2716	8957-8958	CD	denotes	2
T2717	8959-8961	NN	denotes	μl
T2718	8962-8964	IN	denotes	of
T2719	8965-8967	CD	denotes	50
T2720	8967-8968	NN	denotes	%
T2721	8969-8977	NN	denotes	glycerol
T2722	8977-8979	,	denotes	,
T2723	8979-8982	DT	denotes	the
T2724	8992-9000	NNS	denotes	mixtures
T2725	8983-8991	NN	denotes	reaction
T2726	9001-9005	VBD	denotes	were
T2727	9006-9014	RB	denotes	directly
T2728	9023-9025	IN	denotes	on
T2729	9026-9027	DT	denotes	a
T2730	9043-9046	NN	denotes	gel
T2731	9028-9042	NN	denotes	polyacrylamide
T2732	9047-9057	VBG	denotes	containing
T2733	9058-9059	CD	denotes	6
T2734	9060-9062	NN	denotes	mM
T2735	9073-9080	NN	denotes	acetate
T2736	9063-9072	NN	denotes	magnesium
T2737	9081-9084	CC	denotes	and
T2738	9085-9086	CD	denotes	5
T2739	9086-9087	NN	denotes	%
T2740	9088-9096	NN	denotes	glycerol
T2741	9097-9099	IN	denotes	in
T2742	9100-9103	CD	denotes	0.5
T2743	9105-9108	NN	denotes	TBE
T2744	9103-9104	SYM	denotes	×
T2745	9108-9110	,	denotes	,
T2746	9110-9113	CC	denotes	and
T2747	9114-9129	NN	denotes	electrophoresis
T2748	9134-9143	VBN	denotes	conducted
T2749	9130-9133	VBD	denotes	was
T2750	9144-9146	IN	denotes	at
T2751	9147-9151	NN	denotes	room
T2752	9152-9163	NN	denotes	temperature
T2753	9163-9164	.	denotes	.
T2754	9164-9382	sentence	denotes	For DNA helicase assays, the reaction mixtures of the gel shift assays, as described above, were incubated at 30°C for 15 min, and then the ATP was added at 10 mM, followed by incubation at 37°C for additional 30 min.
T2755	9165-9168	IN	denotes	For
T2756	9262-9271	VBN	denotes	incubated
T2757	9169-9172	NN	denotes	DNA
T2758	9182-9188	NNS	denotes	assays
T2759	9173-9181	NN	denotes	helicase
T2760	9188-9190	,	denotes	,
T2761	9190-9193	DT	denotes	the
T2762	9203-9211	NNS	denotes	mixtures
T2763	9194-9202	NN	denotes	reaction
T2764	9212-9214	IN	denotes	of
T2765	9215-9218	DT	denotes	the
T2766	9229-9235	NNS	denotes	assays
T2767	9219-9222	NN	denotes	gel
T2768	9223-9228	NN	denotes	shift
T2769	9235-9237	,	denotes	,
T2770	9237-9239	IN	denotes	as
T2771	9240-9249	VBN	denotes	described
T2772	9250-9255	RB	denotes	above
T2773	9255-9257	,	denotes	,
T2774	9257-9261	VBD	denotes	were
T2775	9272-9274	IN	denotes	at
T2776	9275-9277	CD	denotes	30
T2777	9277-9279	NN	denotes	°C
T2778	9280-9283	IN	denotes	for
T2779	9284-9286	CD	denotes	15
T2780	9287-9290	NN	denotes	min
T2781	9290-9292	,	denotes	,
T2782	9292-9295	CC	denotes	and
T2783	9296-9300	RB	denotes	then
T2784	9301-9304	DT	denotes	the
T2785	9305-9308	NN	denotes	ATP
T2786	9313-9318	VBN	denotes	added
T2787	9309-9312	VBD	denotes	was
T2788	9319-9321	IN	denotes	at
T2789	9322-9324	CD	denotes	10
T2790	9325-9327	NN	denotes	mM
T2791	9327-9329	,	denotes	,
T2792	9329-9337	VBN	denotes	followed
T2793	9338-9340	IN	denotes	by
T2794	9341-9351	NN	denotes	incubation
T2795	9352-9354	IN	denotes	at
T2796	9355-9357	CD	denotes	37
T2797	9357-9359	NN	denotes	°C
T2798	9360-9363	IN	denotes	for
T2799	9364-9374	JJ	denotes	additional
T2800	9378-9381	NN	denotes	min
T2801	9375-9377	CD	denotes	30
T2802	9381-9382	.	denotes	.
T2803	9382-9517	sentence	denotes	The reactions were terminated by addition of EDTA (20 mM), SDS (0.1%) and 2 μg proteinase K, and were incubated for additional 15 min.
T2804	9383-9386	DT	denotes	The
T2805	9387-9396	NNS	denotes	reactions
T2806	9402-9412	VBN	denotes	terminated
T2807	9397-9401	VBD	denotes	were
T2808	9413-9415	IN	denotes	by
T2809	9416-9424	NN	denotes	addition
T2810	9425-9427	IN	denotes	of
T2811	9428-9432	NN	denotes	EDTA
T2812	9433-9434	-LRB-	denotes	(
T2813	9437-9439	NN	denotes	mM
T2814	9434-9436	CD	denotes	20
T2815	9439-9440	-RRB-	denotes	)
T2816	9440-9442	,	denotes	,
T2817	9442-9445	NN	denotes	SDS
T2818	9446-9447	-LRB-	denotes	(
T2819	9450-9451	NN	denotes	%
T2820	9447-9450	CD	denotes	0.1
T2821	9451-9452	-RRB-	denotes	)
T2822	9453-9456	CC	denotes	and
T2823	9457-9458	CD	denotes	2
T2824	9459-9461	NN	denotes	μg
T2825	9473-9474	NN	denotes	K
T2826	9462-9472	NN	denotes	proteinase
T2827	9474-9476	,	denotes	,
T2828	9476-9479	CC	denotes	and
T2829	9480-9484	VBD	denotes	were
T2830	9485-9494	VBN	denotes	incubated
T2831	9495-9498	IN	denotes	for
T2832	9499-9509	JJ	denotes	additional
T2833	9513-9516	NN	denotes	min
T2834	9510-9512	CD	denotes	15
T2835	9516-9517	.	denotes	.
T2836	9517-9610	sentence	denotes	The samples were separated by electrophoresis on nondenaturing polyacrylamide gel in 1× TBE.
T2837	9518-9521	DT	denotes	The
T2838	9522-9529	NNS	denotes	samples
T2839	9535-9544	VBN	denotes	separated
T2840	9530-9534	VBD	denotes	were
T2841	9545-9547	IN	denotes	by
T2842	9548-9563	NN	denotes	electrophoresis
T2843	9564-9566	IN	denotes	on
T2844	9567-9580	JJ	denotes	nondenaturing
T2845	9596-9599	NN	denotes	gel
T2846	9581-9595	NN	denotes	polyacrylamide
T2847	9600-9602	IN	denotes	in
T2848	9603-9604	CD	denotes	1
T2849	9606-9609	NN	denotes	TBE
T2850	9604-9605	SYM	denotes	×
T2851	9609-9610	.	denotes	.
T2967	9612-9620	NN	denotes	Nuclease
T2968	9621-9631	NN	denotes	protection
T2969	9632-9638	NNS	denotes	assays
T2970	9638-9890	sentence	denotes	The reaction mixtures (25 μl) contained 25 mM HEPES–NaOH (pH 7.5), 50 mM sodium acetate, 10 mM Mg(CH3COO)2, 1 mM DTT, 0.25 mg/ml BSA, 0.5 mM ATP-γ-S, 0.5 mM CaCl2, 10 fmol of 32P-labeled bubble substrate, and the indicated amount of Mcm4/6/7 proteins.
T2971	9639-9642	DT	denotes	The
T2972	9652-9660	NNS	denotes	mixtures
T2973	9643-9651	NN	denotes	reaction
T2974	9669-9678	VBD	denotes	contained
T2975	9661-9662	-LRB-	denotes	(
T2976	9665-9667	NN	denotes	μl
T2977	9662-9664	CD	denotes	25
T2978	9667-9668	-RRB-	denotes	)
T2979	9679-9681	CD	denotes	25
T2980	9682-9684	NN	denotes	mM
T2981	9691-9695	NN	denotes	NaOH
T2982	9685-9690	NN	denotes	HEPES
T2983	9690-9691	HYPH	denotes	–
T2984	9696-9697	-LRB-	denotes	(
T2985	9697-9699	NN	denotes	pH
T2986	9700-9703	CD	denotes	7.5
T2987	9703-9704	-RRB-	denotes	)
T2988	9704-9706	,	denotes	,
T2989	9706-9708	CD	denotes	50
T2990	9709-9711	NN	denotes	mM
T2991	9719-9726	NN	denotes	acetate
T2992	9712-9718	NN	denotes	sodium
T2993	9726-9728	,	denotes	,
T2994	9728-9730	CD	denotes	10
T2995	9731-9733	NN	denotes	mM
T2996	9737-9743	NN	denotes	CH3COO
T2997	9734-9736	NN	denotes	Mg
T2998	9736-9737	-LRB-	denotes	(
T2999	9743-9744	-RRB-	denotes	)
T3000	9744-9745	CD	denotes	2
T3001	9745-9747	,	denotes	,
T3002	9747-9748	CD	denotes	1
T3003	9749-9751	NN	denotes	mM
T3004	9752-9755	NN	denotes	DTT
T3005	9755-9757	,	denotes	,
T3006	9757-9761	CD	denotes	0.25
T3007	9762-9764	NN	denotes	mg
T3008	9768-9771	NN	denotes	BSA
T3009	9764-9765	SYM	denotes	/
T3010	9765-9767	NN	denotes	ml
T3011	9771-9773	,	denotes	,
T3012	9773-9776	CD	denotes	0.5
T3013	9777-9779	NN	denotes	mM
T3014	9786-9787	NN	denotes	S
T3015	9780-9783	NN	denotes	ATP
T3016	9783-9784	HYPH	denotes	-
T3017	9784-9785	SYM	denotes	γ
T3018	9785-9786	HYPH	denotes	-
T3019	9787-9789	,	denotes	,
T3020	9789-9792	CD	denotes	0.5
T3021	9793-9795	NN	denotes	mM
T3022	9796-9801	NN	denotes	CaCl2
T3023	9801-9803	,	denotes	,
T3024	9803-9805	CD	denotes	10
T3025	9806-9810	NN	denotes	fmol
T3026	9811-9813	IN	denotes	of
T3027	9814-9817	NN	denotes	32P
T3028	9818-9825	VBN	denotes	labeled
T3029	9817-9818	HYPH	denotes	-
T3030	9833-9842	NN	denotes	substrate
T3031	9826-9832	NN	denotes	bubble
T3032	9842-9844	,	denotes	,
T3033	9844-9847	CC	denotes	and
T3034	9848-9851	DT	denotes	the
T3035	9862-9868	NN	denotes	amount
T3036	9852-9861	VBN	denotes	indicated
T3037	9869-9871	IN	denotes	of
T3038	9872-9876	NN	denotes	Mcm4
T3039	9881-9889	NN	denotes	proteins
T3040	9876-9877	HYPH	denotes	/
T3041	9877-9878	CD	denotes	6
T3042	9878-9879	HYPH	denotes	/
T3043	9879-9880	CD	denotes	7
T3044	9889-9890	.	denotes	.
T3045	9890-10060	sentence	denotes	After incubation at 30°C for 30 min, the indicated amount of DNase I (TAKARA Biomedical, Japan) was added, and the mixtures were incubated at room temperature for 1 min.
T3046	9891-9896	IN	denotes	After
T3047	9991-9996	VBN	denotes	added
T3048	9897-9907	NN	denotes	incubation
T3049	9908-9910	IN	denotes	at
T3050	9911-9913	CD	denotes	30
T3051	9913-9915	NN	denotes	°C
T3052	9916-9919	IN	denotes	for
T3053	9920-9922	CD	denotes	30
T3054	9923-9926	NN	denotes	min
T3055	9926-9928	,	denotes	,
T3056	9928-9931	DT	denotes	the
T3057	9942-9948	NN	denotes	amount
T3058	9932-9941	VBN	denotes	indicated
T3059	9949-9951	IN	denotes	of
T3060	9952-9957	NN	denotes	DNase
T3061	9958-9959	CD	denotes	I
T3062	9960-9961	-LRB-	denotes	(
T3063	9968-9978	NNP	denotes	Biomedical
T3064	9961-9967	NNP	denotes	TAKARA
T3065	9978-9980	,	denotes	,
T3066	9980-9985	NNP	denotes	Japan
T3067	9985-9986	-RRB-	denotes	)
T3068	9987-9990	VBD	denotes	was
T3069	9996-9998	,	denotes	,
T3070	9998-10001	CC	denotes	and
T3071	10002-10005	DT	denotes	the
T3072	10006-10014	NNS	denotes	mixtures
T3073	10020-10029	VBN	denotes	incubated
T3074	10015-10019	VBD	denotes	were
T3075	10030-10032	IN	denotes	at
T3076	10033-10037	NN	denotes	room
T3077	10038-10049	NN	denotes	temperature
T3078	10050-10053	IN	denotes	for
T3079	10054-10055	CD	denotes	1
T3080	10056-10059	NN	denotes	min
T3081	10059-10060	.	denotes	.
T3082	10060-10196	sentence	denotes	DNase I was inactivated by the addition of 250 μl of stop solution (20 mM EDTA, 0.2M sodium chloride, 1% SDS and 12.5 μg/ml yeast RNA).
T3083	10061-10066	NN	denotes	DNase
T3084	10073-10084	VBN	denotes	inactivated
T3085	10067-10068	CD	denotes	I
T3086	10069-10072	VBD	denotes	was
T3087	10085-10087	IN	denotes	by
T3088	10088-10091	DT	denotes	the
T3089	10092-10100	NN	denotes	addition
T3090	10101-10103	IN	denotes	of
T3091	10104-10107	CD	denotes	250
T3092	10108-10110	NN	denotes	μl
T3093	10111-10113	IN	denotes	of
T3094	10114-10118	NN	denotes	stop
T3095	10119-10127	NN	denotes	solution
T3096	10128-10129	-LRB-	denotes	(
T3097	10135-10139	NN	denotes	EDTA
T3098	10129-10131	CD	denotes	20
T3099	10132-10134	NN	denotes	mM
T3100	10139-10141	,	denotes	,
T3101	10141-10145	NN	denotes	0.2M
T3102	10153-10161	NN	denotes	chloride
T3103	10146-10152	NN	denotes	sodium
T3104	10161-10163	,	denotes	,
T3105	10163-10164	CD	denotes	1
T3106	10164-10165	NN	denotes	%
T3107	10166-10169	NN	denotes	SDS
T3108	10170-10173	CC	denotes	and
T3109	10174-10178	CD	denotes	12.5
T3110	10179-10181	NN	denotes	μg
T3111	10191-10194	NN	denotes	RNA
T3112	10181-10182	SYM	denotes	/
T3113	10182-10184	NN	denotes	ml
T3114	10185-10190	NN	denotes	yeast
T3115	10194-10195	-RRB-	denotes	)
T3116	10195-10196	.	denotes	.
T3117	10196-10324	sentence	denotes	For nuclease P1 footprinting assay, binding reactions were carried out under the same conditions except that CaCl2 was omitted.
T3118	10197-10200	IN	denotes	For
T3119	10256-10263	VBN	denotes	carried
T3120	10201-10209	NN	denotes	nuclease
T3121	10213-10225	NN	denotes	footprinting
T3122	10210-10212	NN	denotes	P1
T3123	10226-10231	NN	denotes	assay
T3124	10231-10233	,	denotes	,
T3125	10233-10240	NN	denotes	binding
T3126	10241-10250	NNS	denotes	reactions
T3127	10251-10255	VBD	denotes	were
T3128	10264-10267	RP	denotes	out
T3129	10268-10273	IN	denotes	under
T3130	10274-10277	DT	denotes	the
T3131	10283-10293	NNS	denotes	conditions
T3132	10278-10282	JJ	denotes	same
T3133	10294-10300	IN	denotes	except
T3134	10301-10305	IN	denotes	that
T3135	10316-10323	VBN	denotes	omitted
T3136	10306-10311	NN	denotes	CaCl2
T3137	10312-10315	VBD	denotes	was
T3138	10323-10324	.	denotes	.
T3139	10324-10409	sentence	denotes	P1 nuclease (0.3 U, Roche) was added and incubation was continued at 37°C for 1 min.
T3140	10325-10327	NN	denotes	P1
T3141	10328-10336	NN	denotes	nuclease
T3142	10356-10361	VBN	denotes	added
T3143	10337-10338	-LRB-	denotes	(
T3144	10345-10350	NNP	denotes	Roche
T3145	10338-10341	CD	denotes	0.3
T3146	10342-10343	NNP	denotes	U
T3147	10343-10345	,	denotes	,
T3148	10350-10351	-RRB-	denotes	)
T3149	10352-10355	VBD	denotes	was
T3150	10362-10365	CC	denotes	and
T3151	10366-10376	NN	denotes	incubation
T3152	10381-10390	VBN	denotes	continued
T3153	10377-10380	VBD	denotes	was
T3154	10391-10393	IN	denotes	at
T3155	10394-10396	CD	denotes	37
T3156	10396-10398	NN	denotes	°C
T3157	10399-10402	IN	denotes	for
T3158	10403-10404	CD	denotes	1
T3159	10405-10408	NN	denotes	min
T3160	10408-10409	.	denotes	.
T3161	10409-10604	sentence	denotes	Cleavage was stopped by addition of 50 μl of 25 mM EDTA and 0.4 M NaOH; the mixture was incubated at room temperature for 10 min followed by the addition of 12 μl of 3 M sodium acetate (pH 4.8).
T3162	10410-10418	NN	denotes	Cleavage
T3163	10423-10430	VBN	denotes	stopped
T3164	10419-10422	VBD	denotes	was
T3165	10498-10507	VBN	denotes	incubated
T3166	10431-10433	IN	denotes	by
T3167	10434-10442	NN	denotes	addition
T3168	10443-10445	IN	denotes	of
T3169	10446-10448	CD	denotes	50
T3170	10449-10451	NN	denotes	μl
T3171	10452-10454	IN	denotes	of
T3172	10455-10457	CD	denotes	25
T3173	10458-10460	NN	denotes	mM
T3174	10461-10465	NN	denotes	EDTA
T3175	10466-10469	CC	denotes	and
T3176	10470-10473	CD	denotes	0.4
T3177	10474-10475	NN	denotes	M
T3178	10476-10480	NN	denotes	NaOH
T3179	10480-10481	:	denotes	;
T3180	10482-10485	DT	denotes	the
T3181	10486-10493	NN	denotes	mixture
T3182	10494-10497	VBD	denotes	was
T3183	10508-10510	IN	denotes	at
T3184	10511-10515	NN	denotes	room
T3185	10516-10527	NN	denotes	temperature
T3186	10528-10531	IN	denotes	for
T3187	10532-10534	CD	denotes	10
T3188	10535-10538	NN	denotes	min
T3189	10539-10547	VBN	denotes	followed
T3190	10548-10550	IN	denotes	by
T3191	10551-10554	DT	denotes	the
T3192	10555-10563	NN	denotes	addition
T3193	10564-10566	IN	denotes	of
T3194	10567-10569	CD	denotes	12
T3195	10570-10572	NN	denotes	μl
T3196	10573-10575	IN	denotes	of
T3197	10576-10577	CD	denotes	3
T3198	10578-10579	NN	denotes	M
T3199	10587-10594	NN	denotes	acetate
T3200	10580-10586	NN	denotes	sodium
T3201	10595-10596	-LRB-	denotes	(
T3202	10596-10598	NN	denotes	pH
T3203	10599-10602	CD	denotes	4.8
T3204	10602-10603	-RRB-	denotes	)
T3205	10603-10604	.	denotes	.
T3206	10604-10743	sentence	denotes	Proteins were removed by phenol–chloroform extraction, and DNAs were collected by ethanol precipitation followed by wash with 70% ethanol.
T3207	10605-10613	NNS	denotes	Proteins
T3208	10619-10626	VBN	denotes	removed
T3209	10614-10618	VBD	denotes	were
T3210	10627-10629	IN	denotes	by
T3211	10630-10636	NN	denotes	phenol
T3212	10637-10647	NN	denotes	chloroform
T3213	10636-10637	HYPH	denotes	–
T3214	10648-10658	NN	denotes	extraction
T3215	10658-10660	,	denotes	,
T3216	10660-10663	CC	denotes	and
T3217	10664-10668	NNS	denotes	DNAs
T3218	10674-10683	VBN	denotes	collected
T3219	10669-10673	VBD	denotes	were
T3220	10684-10686	IN	denotes	by
T3221	10687-10694	NN	denotes	ethanol
T3222	10695-10708	NN	denotes	precipitation
T3223	10709-10717	VBN	denotes	followed
T3224	10718-10720	IN	denotes	by
T3225	10721-10725	NN	denotes	wash
T3226	10726-10730	IN	denotes	with
T3227	10731-10733	CD	denotes	70
T3228	10733-10734	NN	denotes	%
T3229	10735-10742	NN	denotes	ethanol
T3230	10742-10743	.	denotes	.
T3231	10743-10893	sentence	denotes	The digested DNA was subjected to electrophoresis through a 10 or 12% polyacrylamide sequencing gel containing 7 M urea, followed by autoradiography.
T3232	10744-10747	DT	denotes	The
T3233	10757-10760	NN	denotes	DNA
T3234	10748-10756	VBN	denotes	digested
T3235	10765-10774	VBN	denotes	subjected
T3236	10761-10764	VBD	denotes	was
T3237	10775-10777	IN	denotes	to
T3238	10778-10793	NN	denotes	electrophoresis
T3239	10794-10801	IN	denotes	through
T3240	10802-10803	DT	denotes	a
T3241	10840-10843	NN	denotes	gel
T3242	10804-10806	CD	denotes	10
T3243	10812-10813	NN	denotes	%
T3244	10807-10809	CC	denotes	or
T3245	10810-10812	CD	denotes	12
T3246	10814-10828	NN	denotes	polyacrylamide
T3247	10829-10839	NN	denotes	sequencing
T3248	10844-10854	VBG	denotes	containing
T3249	10855-10856	CD	denotes	7
T3250	10857-10858	NN	denotes	M
T3251	10859-10863	NN	denotes	urea
T3252	10863-10865	,	denotes	,
T3253	10865-10873	VBN	denotes	followed
T3254	10874-10876	IN	denotes	by
T3255	10877-10892	NN	denotes	autoradiography
T3256	10892-10893	.	denotes	.
T3477	11197-11206	VBD	denotes	conducted
T3428	10904-10908	NN	denotes	Mcm4
T3429	10913-10920	NN	denotes	complex
T3430	10908-10909	HYPH	denotes	/
T3431	10909-10910	CD	denotes	6
T3432	10910-10911	HYPH	denotes	/
T3433	10911-10912	CD	denotes	7
T3434	10931-10940	VBZ	denotes	interacts
T3435	10921-10930	RB	denotes	primarily
T3436	10941-10945	IN	denotes	with
T3437	10946-10949	DT	denotes	the
T3438	10970-10977	NN	denotes	segment
T3439	10950-10956	JJ	denotes	single
T3440	10957-10965	VBN	denotes	stranded
T3441	10956-10957	HYPH	denotes	-
T3442	10966-10969	NN	denotes	DNA
T3443	10977-11125	sentence	denotes	Mcm4/6/7 binds to ‘bubble’ DNA substrates and unwinds the duplex segments when the central single-stranded segment contains thymine stretches (13).
T3444	10978-10982	NN	denotes	Mcm4
T3445	10987-10992	VBZ	denotes	binds
T3446	10982-10983	HYPH	denotes	/
T3447	10983-10984	CD	denotes	6
T3448	10984-10985	HYPH	denotes	/
T3449	10985-10986	CD	denotes	7
T3450	10993-10995	IN	denotes	to
T3451	10996-10997	``	denotes	‘
T3452	11009-11019	NNS	denotes	substrates
T3453	10997-11003	NN	denotes	bubble
T3454	11003-11004	''	denotes	’
T3455	11005-11008	NN	denotes	DNA
T3456	11020-11023	CC	denotes	and
T3457	11024-11031	VBZ	denotes	unwinds
T3458	11032-11035	DT	denotes	the
T3459	11043-11051	NNS	denotes	segments
T3460	11036-11042	NN	denotes	duplex
T3461	11052-11056	WRB	denotes	when
T3462	11093-11101	VBZ	denotes	contains
T3463	11057-11060	DT	denotes	the
T3464	11085-11092	NN	denotes	segment
T3465	11061-11068	JJ	denotes	central
T3466	11069-11075	JJ	denotes	single
T3467	11076-11084	VBN	denotes	stranded
T3468	11075-11076	HYPH	denotes	-
T3469	11102-11109	NN	denotes	thymine
T3470	11110-11119	NNS	denotes	stretches
T3471	11120-11121	-LRB-	denotes	(
T3472	11121-11123	CD	denotes	13
T3473	11123-11124	-RRB-	denotes	)
T3474	11124-11125	.	denotes	.
T3475	11125-11384	sentence	denotes	In order to examine the mode of binding of Mcm4/6/7 on bubble DNAs, we conducted nuclease protection assays using labeled bubble substrate DNAs and Mcm4/6/7 in the presence of ATP-γ-S which permits binding to substrate DNA but does not mobilize the helicase.
T3476	11126-11128	IN	denotes	In
T3478	11129-11134	NN	denotes	order
T3479	11135-11137	TO	denotes	to
T3480	11138-11145	VB	denotes	examine
T3481	11146-11149	DT	denotes	the
T3482	11150-11154	NN	denotes	mode
T3483	11155-11157	IN	denotes	of
T3484	11158-11165	NN	denotes	binding
T3485	11166-11168	IN	denotes	of
T3486	11169-11173	NN	denotes	Mcm4
T3487	11173-11174	HYPH	denotes	/
T3488	11174-11175	CD	denotes	6
T3489	11175-11176	HYPH	denotes	/
T3490	11176-11177	CD	denotes	7
T3491	11178-11180	IN	denotes	on
T3492	11181-11187	NN	denotes	bubble
T3493	11188-11192	NNS	denotes	DNAs
T3494	11192-11194	,	denotes	,
T3495	11194-11196	PRP	denotes	we
T3496	11207-11215	NN	denotes	nuclease
T3497	11227-11233	NNS	denotes	assays
T3498	11216-11226	NN	denotes	protection
T3499	11234-11239	VBG	denotes	using
T3500	11240-11247	VBN	denotes	labeled
T3501	11265-11269	NNS	denotes	DNAs
T3502	11248-11254	NN	denotes	bubble
T3503	11255-11264	NN	denotes	substrate
T3504	11270-11273	CC	denotes	and
T3505	11274-11278	NN	denotes	Mcm4
T3506	11278-11279	HYPH	denotes	/
T3507	11279-11280	CD	denotes	6
T3508	11280-11281	HYPH	denotes	/
T3509	11281-11282	CD	denotes	7
T3510	11283-11285	IN	denotes	in
T3511	11286-11289	DT	denotes	the
T3512	11290-11298	NN	denotes	presence
T3513	11299-11301	IN	denotes	of
T3514	11302-11305	NN	denotes	ATP
T3515	11308-11309	NN	denotes	S
T3516	11305-11306	HYPH	denotes	-
T3517	11306-11307	SYM	denotes	γ
T3518	11307-11308	HYPH	denotes	-
T3519	11310-11315	WDT	denotes	which
T3520	11316-11323	VBZ	denotes	permits
T3521	11324-11331	NN	denotes	binding
T3522	11332-11334	IN	denotes	to
T3523	11335-11344	NN	denotes	substrate
T3524	11345-11348	NN	denotes	DNA
T3525	11349-11352	CC	denotes	but
T3526	11353-11357	VBZ	denotes	does
T3527	11362-11370	VB	denotes	mobilize
T3528	11358-11361	RB	denotes	not
T3529	11371-11374	DT	denotes	the
T3530	11375-11383	NN	denotes	helicase
T3531	11383-11384	.	denotes	.
T3532	11384-11655	sentence	denotes	At an optimum concentration of DNase I, strong cleavages were detected on the 21 bp duplex regions at both ends of the substrates, whereas weaker cleavages were detected on the central single-stranded region, consistent with preference of DNase I to double-stranded DNA.
T3533	11385-11387	IN	denotes	At
T3534	11447-11455	VBN	denotes	detected
T3535	11388-11390	DT	denotes	an
T3536	11399-11412	NN	denotes	concentration
T3537	11391-11398	JJ	denotes	optimum
T3538	11413-11415	IN	denotes	of
T3539	11416-11421	NN	denotes	DNase
T3540	11422-11423	CD	denotes	I
T3541	11423-11425	,	denotes	,
T3542	11425-11431	JJ	denotes	strong
T3543	11432-11441	NNS	denotes	cleavages
T3544	11442-11446	VBD	denotes	were
T3545	11456-11458	IN	denotes	on
T3546	11459-11462	DT	denotes	the
T3547	11476-11483	NNS	denotes	regions
T3548	11463-11465	CD	denotes	21
T3549	11466-11468	NN	denotes	bp
T3550	11469-11475	NN	denotes	duplex
T3551	11484-11486	IN	denotes	at
T3552	11487-11491	DT	denotes	both
T3553	11492-11496	NNS	denotes	ends
T3554	11497-11499	IN	denotes	of
T3555	11500-11503	DT	denotes	the
T3556	11504-11514	NNS	denotes	substrates
T3557	11514-11516	,	denotes	,
T3558	11516-11523	IN	denotes	whereas
T3559	11546-11554	VBN	denotes	detected
T3560	11524-11530	JJR	denotes	weaker
T3561	11531-11540	NNS	denotes	cleavages
T3562	11541-11545	VBD	denotes	were
T3563	11555-11557	IN	denotes	on
T3564	11558-11561	DT	denotes	the
T3565	11586-11592	NN	denotes	region
T3566	11562-11569	JJ	denotes	central
T3567	11570-11576	JJ	denotes	single
T3568	11577-11585	VBN	denotes	stranded
T3569	11576-11577	HYPH	denotes	-
T3570	11592-11594	,	denotes	,
T3571	11594-11604	JJ	denotes	consistent
T3572	11605-11609	IN	denotes	with
T3573	11610-11620	NN	denotes	preference
T3574	11621-11623	IN	denotes	of
T3575	11624-11629	NN	denotes	DNase
T3576	11630-11631	CD	denotes	I
T3577	11632-11634	IN	denotes	to
T3578	11635-11641	JJ	denotes	double
T3579	11642-11650	VBN	denotes	stranded
T3580	11641-11642	HYPH	denotes	-
T3581	11651-11654	NN	denotes	DNA
T3582	11654-11655	.	denotes	.
T3583	11655-11913	sentence	denotes	With the Bub66/T-rich substrate, strong protection was detected on the entire single-stranded regions of both top and bottom strands with increasing concentration of Mcm4/6/7, consistent with high affinity of Mcm4/6/7 to T-rich bubble sequences (Figure 1A).
T3584	11656-11660	IN	denotes	With
T3585	11711-11719	VBN	denotes	detected
T3586	11661-11664	DT	denotes	the
T3587	11678-11687	NN	denotes	substrate
T3588	11665-11670	NN	denotes	Bub66
T3589	11670-11671	HYPH	denotes	/
T3590	11671-11672	NN	denotes	T
T3591	11672-11673	HYPH	denotes	-
T3592	11673-11677	JJ	denotes	rich
T3593	11687-11689	,	denotes	,
T3594	11689-11695	JJ	denotes	strong
T3595	11696-11706	NN	denotes	protection
T3596	11707-11710	VBD	denotes	was
T3597	11720-11722	IN	denotes	on
T3598	11723-11726	DT	denotes	the
T3599	11750-11757	NNS	denotes	regions
T3600	11727-11733	JJ	denotes	entire
T3601	11734-11740	JJ	denotes	single
T3602	11741-11749	VBN	denotes	stranded
T3603	11740-11741	HYPH	denotes	-
T3604	11758-11760	IN	denotes	of
T3605	11761-11765	CC	denotes	both
T3606	11766-11769	JJ	denotes	top
T3607	11781-11788	NNS	denotes	strands
T3608	11770-11773	CC	denotes	and
T3609	11774-11780	JJ	denotes	bottom
T3610	11789-11793	IN	denotes	with
T3611	11794-11804	VBG	denotes	increasing
T3612	11805-11818	NN	denotes	concentration
T3613	11819-11821	IN	denotes	of
T3614	11822-11826	NN	denotes	Mcm4
T3615	11826-11827	HYPH	denotes	/
T3616	11827-11828	CD	denotes	6
T3617	11828-11829	HYPH	denotes	/
T3618	11829-11830	CD	denotes	7
T3619	11830-11832	,	denotes	,
T3620	11832-11842	JJ	denotes	consistent
T3621	11843-11847	IN	denotes	with
T3622	11848-11852	JJ	denotes	high
T3623	11853-11861	NN	denotes	affinity
T3624	11862-11864	IN	denotes	of
T3625	11865-11869	NN	denotes	Mcm4
T3626	11869-11870	HYPH	denotes	/
T3627	11870-11871	CD	denotes	6
T3628	11871-11872	HYPH	denotes	/
T3629	11872-11873	CD	denotes	7
T3630	11874-11876	IN	denotes	to
T3631	11877-11878	NN	denotes	T
T3632	11891-11900	NNS	denotes	sequences
T3633	11878-11879	HYPH	denotes	-
T3634	11879-11883	JJ	denotes	rich
T3635	11884-11890	NN	denotes	bubble
T3636	11901-11902	-LRB-	denotes	(
T3637	11909-11911	NN	denotes	1A
T3638	11902-11908	NN	denotes	Figure
T3639	11911-11912	-RRB-	denotes	)
T3640	11912-11913	.	denotes	.
T3641	11913-12075	sentence	denotes	Similar, but less significant protection was detected on the single-stranded DNA segment with P1 nucleases (Figure 1A), which is specific to single-stranded DNA.
T3642	11914-11921	JJ	denotes	Similar
T3643	11944-11954	NN	denotes	protection
T3644	11921-11923	,	denotes	,
T3645	11923-11926	CC	denotes	but
T3646	11927-11931	RBR	denotes	less
T3647	11932-11943	JJ	denotes	significant
T3648	11959-11967	VBN	denotes	detected
T3649	11955-11958	VBD	denotes	was
T3650	11968-11970	IN	denotes	on
T3651	11971-11974	DT	denotes	the
T3652	11995-12002	NN	denotes	segment
T3653	11975-11981	JJ	denotes	single
T3654	11982-11990	VBN	denotes	stranded
T3655	11981-11982	HYPH	denotes	-
T3656	11991-11994	NN	denotes	DNA
T3657	12003-12007	IN	denotes	with
T3658	12008-12010	NN	denotes	P1
T3659	12011-12020	NNS	denotes	nucleases
T3660	12021-12022	-LRB-	denotes	(
T3661	12029-12031	NN	denotes	1A
T3662	12022-12028	NN	denotes	Figure
T3663	12031-12032	-RRB-	denotes	)
T3664	12032-12034	,	denotes	,
T3665	12034-12039	WDT	denotes	which
T3666	12040-12042	VBZ	denotes	is
T3667	12043-12051	JJ	denotes	specific
T3668	12052-12054	IN	denotes	to
T3669	12055-12061	JJ	denotes	single
T3670	12062-12070	VBN	denotes	stranded
T3671	12061-12062	HYPH	denotes	-
T3672	12071-12074	NN	denotes	DNA
T3673	12074-12075	.	denotes	.
T3674	12075-12206	sentence	denotes	The results indicate that the Mcm complex is loaded onto bubble DNA through binding to both strands of the single-stranded region.
T3675	12076-12079	DT	denotes	The
T3676	12080-12087	NNS	denotes	results
T3677	12088-12096	VBP	denotes	indicate
T3678	12097-12101	IN	denotes	that
T3679	12121-12127	VBN	denotes	loaded
T3680	12102-12105	DT	denotes	the
T3681	12110-12117	NN	denotes	complex
T3682	12106-12109	NN	denotes	Mcm
T3683	12118-12120	VBZ	denotes	is
T3684	12128-12132	IN	denotes	onto
T3685	12133-12139	NN	denotes	bubble
T3686	12140-12143	NN	denotes	DNA
T3687	12144-12151	IN	denotes	through
T3688	12152-12159	NN	denotes	binding
T3689	12160-12162	IN	denotes	to
T3690	12163-12167	DT	denotes	both
T3691	12168-12175	NNS	denotes	strands
T3692	12176-12178	IN	denotes	of
T3693	12179-12182	DT	denotes	the
T3694	12199-12205	NN	denotes	region
T3695	12183-12189	JJ	denotes	single
T3696	12190-12198	VBN	denotes	stranded
T3697	12189-12190	HYPH	denotes	-
T3698	12205-12206	.	denotes	.
T3699	12206-12480	sentence	denotes	Careful examination of Mcm4/6/7 footprints on a bubble indicated strong protection on the 5′-half of the top strand and moderate protection on the remaining 3′-half, as well as on several base-pair duplex segment adjacent to the branch point (Figure 1A and data not shown).
T3700	12207-12214	JJ	denotes	Careful
T3701	12215-12226	NN	denotes	examination
T3702	12262-12271	VBD	denotes	indicated
T3703	12227-12229	IN	denotes	of
T3704	12230-12234	NN	denotes	Mcm4
T3705	12239-12249	NNS	denotes	footprints
T3706	12234-12235	HYPH	denotes	/
T3707	12235-12236	CD	denotes	6
T3708	12236-12237	HYPH	denotes	/
T3709	12237-12238	CD	denotes	7
T3710	12250-12252	IN	denotes	on
T3711	12253-12254	DT	denotes	a
T3712	12255-12261	NN	denotes	bubble
T3713	12272-12278	JJ	denotes	strong
T3714	12279-12289	NN	denotes	protection
T3715	12290-12292	IN	denotes	on
T3716	12293-12296	DT	denotes	the
T3717	12300-12304	NN	denotes	half
T3718	12297-12298	CD	denotes	5
T3719	12298-12299	SYM	denotes	′
T3720	12299-12300	HYPH	denotes	-
T3721	12305-12307	IN	denotes	of
T3722	12308-12311	DT	denotes	the
T3723	12316-12322	NN	denotes	strand
T3724	12312-12315	JJ	denotes	top
T3725	12323-12326	CC	denotes	and
T3726	12327-12335	JJ	denotes	moderate
T3727	12336-12346	NN	denotes	protection
T3728	12347-12349	IN	denotes	on
T3729	12350-12353	DT	denotes	the
T3730	12367-12371	NN	denotes	half
T3731	12354-12363	VBG	denotes	remaining
T3732	12364-12365	CD	denotes	3
T3733	12365-12366	SYM	denotes	′
T3734	12366-12367	HYPH	denotes	-
T3735	12371-12373	,	denotes	,
T3736	12373-12375	RB	denotes	as
T3737	12381-12383	IN	denotes	as
T3738	12376-12380	RB	denotes	well
T3739	12384-12386	IN	denotes	on
T3740	12387-12394	JJ	denotes	several
T3741	12412-12419	NN	denotes	segment
T3742	12395-12399	NN	denotes	base
T3743	12400-12404	NN	denotes	pair
T3744	12399-12400	HYPH	denotes	-
T3745	12405-12411	NN	denotes	duplex
T3746	12420-12428	JJ	denotes	adjacent
T3747	12429-12431	IN	denotes	to
T3748	12432-12435	DT	denotes	the
T3749	12443-12448	NN	denotes	point
T3750	12436-12442	NN	denotes	branch
T3751	12449-12450	-LRB-	denotes	(
T3752	12457-12459	NN	denotes	1A
T3753	12450-12456	NN	denotes	Figure
T3754	12460-12463	CC	denotes	and
T3755	12464-12468	NNS	denotes	data
T3756	12469-12472	RB	denotes	not
T3757	12473-12478	VBN	denotes	shown
T3758	12478-12479	-RRB-	denotes	)
T3759	12479-12480	.	denotes	.
T3760	12480-12552	sentence	denotes	A similar pattern of protection was observed also on the bottom strand.
T3761	12481-12482	DT	denotes	A
T3762	12491-12498	NN	denotes	pattern
T3763	12483-12490	JJ	denotes	similar
T3764	12517-12525	VBN	denotes	observed
T3765	12499-12501	IN	denotes	of
T3766	12502-12512	NN	denotes	protection
T3767	12513-12516	VBD	denotes	was
T3768	12526-12530	RB	denotes	also
T3769	12531-12533	IN	denotes	on
T3770	12534-12537	DT	denotes	the
T3771	12545-12551	NN	denotes	strand
T3772	12538-12544	JJ	denotes	bottom
T3773	12551-12552	.	denotes	.
T3774	12552-12745	sentence	denotes	This suggests that Mcm4/6/7 may bind to a replication bubble substrate with a 2-fold symmetry as a double hexamer (Figure 1A, see the lower scheme), in a manner similar to SV40 T-antigen (21).
T3775	12553-12557	DT	denotes	This
T3776	12558-12566	VBZ	denotes	suggests
T3777	12567-12571	IN	denotes	that
T3778	12585-12589	VB	denotes	bind
T3779	12572-12576	NN	denotes	Mcm4
T3780	12576-12577	HYPH	denotes	/
T3781	12577-12578	CD	denotes	6
T3782	12578-12579	HYPH	denotes	/
T3783	12579-12580	CD	denotes	7
T3784	12581-12584	MD	denotes	may
T3785	12590-12592	IN	denotes	to
T3786	12593-12594	DT	denotes	a
T3787	12614-12623	NN	denotes	substrate
T3788	12595-12606	NN	denotes	replication
T3789	12607-12613	NN	denotes	bubble
T3790	12624-12628	IN	denotes	with
T3791	12629-12630	DT	denotes	a
T3792	12638-12646	NN	denotes	symmetry
T3793	12631-12637	CD	denotes	2-fold
T3794	12647-12649	IN	denotes	as
T3795	12650-12651	DT	denotes	a
T3796	12659-12666	NN	denotes	hexamer
T3797	12652-12658	JJ	denotes	double
T3798	12667-12668	-LRB-	denotes	(
T3799	12675-12677	NN	denotes	1A
T3800	12668-12674	NN	denotes	Figure
T3801	12677-12679	,	denotes	,
T3802	12679-12682	VBP	denotes	see
T3803	12683-12686	DT	denotes	the
T3804	12693-12699	NN	denotes	scheme
T3805	12687-12692	JJ	denotes	lower
T3806	12699-12700	-RRB-	denotes	)
T3807	12700-12702	,	denotes	,
T3808	12702-12704	IN	denotes	in
T3809	12705-12706	DT	denotes	a
T3810	12707-12713	NN	denotes	manner
T3811	12714-12721	JJ	denotes	similar
T3812	12722-12724	IN	denotes	to
T3813	12725-12729	NN	denotes	SV40
T3814	12732-12739	NN	denotes	antigen
T3815	12730-12731	NN	denotes	T
T3816	12731-12732	HYPH	denotes	-
T3817	12740-12741	-LRB-	denotes	(
T3818	12741-12743	CD	denotes	21
T3819	12743-12744	-RRB-	denotes	)
T3820	12744-12745	.	denotes	.
T3821	12745-12836	sentence	denotes	Nuclease protection assays were conducted also on a T-tailed Y-fork structure (Figure 1B).
T3822	12746-12754	NN	denotes	Nuclease
T3823	12755-12765	NN	denotes	protection
T3824	12766-12772	NNS	denotes	assays
T3825	12778-12787	VBN	denotes	conducted
T3826	12773-12777	VBD	denotes	were
T3827	12788-12792	RB	denotes	also
T3828	12793-12795	IN	denotes	on
T3829	12796-12797	DT	denotes	a
T3830	12814-12823	NN	denotes	structure
T3831	12798-12799	NN	denotes	T
T3832	12800-12806	VBN	denotes	tailed
T3833	12799-12800	HYPH	denotes	-
T3834	12807-12808	NN	denotes	Y
T3835	12809-12813	NN	denotes	fork
T3836	12808-12809	HYPH	denotes	-
T3837	12824-12825	-LRB-	denotes	(
T3838	12832-12834	NN	denotes	1B
T3839	12825-12831	NN	denotes	Figure
T3840	12834-12835	-RRB-	denotes	)
T3841	12835-12836	.	denotes	.
T3842	12836-13008	sentence	denotes	Not only the single-stranded tail region but also the 7 bp duplex DNA adjacent to the duplex-to-single-strand junction were protected by Mcm binding from DNaseI digestion.
T3843	12837-12840	RB	denotes	Not
T3844	12871-12877	NN	denotes	region
T3845	12841-12845	RB	denotes	only
T3846	12846-12849	DT	denotes	the
T3847	12850-12856	JJ	denotes	single
T3848	12857-12865	VBN	denotes	stranded
T3849	12856-12857	HYPH	denotes	-
T3850	12866-12870	NN	denotes	tail
T3851	12961-12970	VBN	denotes	protected
T3852	12878-12881	CC	denotes	but
T3853	12882-12886	RB	denotes	also
T3854	12887-12890	DT	denotes	the
T3855	12903-12906	NN	denotes	DNA
T3856	12891-12892	CD	denotes	7
T3857	12893-12895	NN	denotes	bp
T3858	12896-12902	NN	denotes	duplex
T3859	12907-12915	JJ	denotes	adjacent
T3860	12916-12918	IN	denotes	to
T3861	12919-12922	DT	denotes	the
T3862	12947-12955	NN	denotes	junction
T3863	12923-12929	NN	denotes	duplex
T3864	12929-12930	HYPH	denotes	-
T3865	12930-12932	IN	denotes	to
T3866	12932-12933	HYPH	denotes	-
T3867	12933-12939	JJ	denotes	single
T3868	12940-12946	NN	denotes	strand
T3869	12939-12940	HYPH	denotes	-
T3870	12956-12960	VBD	denotes	were
T3871	12971-12973	IN	denotes	by
T3872	12974-12977	NN	denotes	Mcm
T3873	12978-12985	NN	denotes	binding
T3874	12986-12990	IN	denotes	from
T3875	12991-12997	NN	denotes	DNaseI
T3876	12998-13007	NN	denotes	digestion
T3877	13007-13008	.	denotes	.
T3878	13008-13207	sentence	denotes	These results indicate that Mcm4/6/7 protein primarily interacts with single-stranded DNA region and that the interaction also spans the duplex segments near the branchpoint of the replication fork.
T3879	13009-13014	DT	denotes	These
T3880	13015-13022	NNS	denotes	results
T3881	13023-13031	VBP	denotes	indicate
T3882	13032-13036	IN	denotes	that
T3883	13064-13073	VBZ	denotes	interacts
T3884	13037-13041	NN	denotes	Mcm4
T3885	13046-13053	NN	denotes	protein
T3886	13041-13042	HYPH	denotes	/
T3887	13042-13043	CD	denotes	6
T3888	13043-13044	HYPH	denotes	/
T3889	13044-13045	CD	denotes	7
T3890	13054-13063	RB	denotes	primarily
T3891	13074-13078	IN	denotes	with
T3892	13079-13085	JJ	denotes	single
T3893	13086-13094	VBN	denotes	stranded
T3894	13085-13086	HYPH	denotes	-
T3895	13099-13105	NN	denotes	region
T3896	13095-13098	NN	denotes	DNA
T3897	13106-13109	CC	denotes	and
T3898	13110-13114	IN	denotes	that
T3899	13136-13141	VBZ	denotes	spans
T3900	13115-13118	DT	denotes	the
T3901	13119-13130	NN	denotes	interaction
T3902	13131-13135	RB	denotes	also
T3903	13142-13145	DT	denotes	the
T3904	13153-13161	NNS	denotes	segments
T3905	13146-13152	NN	denotes	duplex
T3906	13162-13166	IN	denotes	near
T3907	13167-13170	DT	denotes	the
T3908	13171-13182	NN	denotes	branchpoint
T3909	13183-13185	IN	denotes	of
T3910	13186-13189	DT	denotes	the
T3911	13202-13206	NN	denotes	fork
T3912	13190-13201	NN	denotes	replication
T3913	13206-13207	.	denotes	.
T4077	13209-13218	NN	denotes	Formation
T4078	13258-13269	VBZ	denotes	facilitates
T4079	13219-13221	IN	denotes	of
T4080	13222-13228	JJ	denotes	double
T4081	13229-13238	JJ	denotes	hexameric
T4082	13248-13257	NNS	denotes	complexes
T4083	13239-13243	NN	denotes	Mcm4
T4084	13243-13244	HYPH	denotes	/
T4085	13244-13245	CD	denotes	6
T4086	13245-13246	HYPH	denotes	/
T4087	13246-13247	CD	denotes	7
T4088	13270-13278	NN	denotes	helicase
T4089	13279-13286	NNS	denotes	actions
T4090	13287-13289	IN	denotes	on
T4091	13290-13296	NN	denotes	bubble
T4092	13297-13307	NNS	denotes	substrates
T4093	13307-13497	sentence	denotes	The above results suggest that Mcm4/6/7 generates a double hexameric complex on a bubble, each hexamer of which makes symmetric contact with the 5′-proximal central single-stranded segment.
T4094	13308-13311	DT	denotes	The
T4095	13318-13325	NNS	denotes	results
T4096	13312-13317	JJ	denotes	above
T4097	13326-13333	VBP	denotes	suggest
T4098	13334-13338	IN	denotes	that
T4099	13348-13357	VBZ	denotes	generates
T4100	13339-13343	NN	denotes	Mcm4
T4101	13343-13344	HYPH	denotes	/
T4102	13344-13345	CD	denotes	6
T4103	13345-13346	HYPH	denotes	/
T4104	13346-13347	CD	denotes	7
T4105	13358-13359	DT	denotes	a
T4106	13377-13384	NN	denotes	complex
T4107	13360-13366	JJ	denotes	double
T4108	13367-13376	JJ	denotes	hexameric
T4109	13385-13387	IN	denotes	on
T4110	13388-13389	DT	denotes	a
T4111	13390-13396	NN	denotes	bubble
T4112	13396-13398	,	denotes	,
T4113	13398-13402	DT	denotes	each
T4114	13403-13410	NN	denotes	hexamer
T4115	13420-13425	VBZ	denotes	makes
T4116	13411-13413	IN	denotes	of
T4117	13414-13419	WDT	denotes	which
T4118	13426-13435	JJ	denotes	symmetric
T4119	13436-13443	NN	denotes	contact
T4120	13444-13448	IN	denotes	with
T4121	13449-13452	DT	denotes	the
T4122	13489-13496	NN	denotes	segment
T4123	13453-13454	CD	denotes	5
T4124	13456-13464	JJ	denotes	proximal
T4125	13454-13455	SYM	denotes	′
T4126	13455-13456	HYPH	denotes	-
T4127	13465-13472	JJ	denotes	central
T4128	13473-13479	JJ	denotes	single
T4129	13480-13488	VBN	denotes	stranded
T4130	13479-13480	HYPH	denotes	-
T4131	13496-13497	.	denotes	.
T4132	13497-13620	sentence	denotes	This predicts that the single-stranded segment needs to be of a sufficient length in order to accommodate the Mcm complex.
T4133	13498-13502	DT	denotes	This
T4134	13503-13511	VBZ	denotes	predicts
T4135	13512-13516	IN	denotes	that
T4136	13545-13550	VBZ	denotes	needs
T4137	13517-13520	DT	denotes	the
T4138	13537-13544	NN	denotes	segment
T4139	13521-13527	JJ	denotes	single
T4140	13528-13536	VBN	denotes	stranded
T4141	13527-13528	HYPH	denotes	-
T4142	13551-13553	TO	denotes	to
T4143	13554-13556	VB	denotes	be
T4144	13557-13559	IN	denotes	of
T4145	13560-13561	DT	denotes	a
T4146	13573-13579	NN	denotes	length
T4147	13562-13572	JJ	denotes	sufficient
T4148	13580-13582	IN	denotes	in
T4149	13583-13588	NN	denotes	order
T4150	13589-13591	TO	denotes	to
T4151	13592-13603	VB	denotes	accommodate
T4152	13604-13607	DT	denotes	the
T4153	13612-13619	NN	denotes	complex
T4154	13608-13611	NN	denotes	Mcm
T4155	13619-13620	.	denotes	.
T4156	13620-13844	sentence	denotes	In fact, we previously showed that the Mcm4/6/7 mainly forms a single hexameric complex on bubble-20 (a bubble substrate with 20 nt long central single-stranded segment) and a double hexameric complex on bubble-60 DNA (13).
T4157	13621-13623	IN	denotes	In
T4158	13644-13650	VBD	denotes	showed
T4159	13624-13628	NN	denotes	fact
T4160	13628-13630	,	denotes	,
T4161	13630-13632	PRP	denotes	we
T4162	13633-13643	RB	denotes	previously
T4163	13651-13655	IN	denotes	that
T4164	13676-13681	VBZ	denotes	forms
T4165	13656-13659	DT	denotes	the
T4166	13660-13664	NN	denotes	Mcm4
T4167	13664-13665	HYPH	denotes	/
T4168	13665-13666	CD	denotes	6
T4169	13666-13667	HYPH	denotes	/
T4170	13667-13668	CD	denotes	7
T4171	13669-13675	RB	denotes	mainly
T4172	13682-13683	DT	denotes	a
T4173	13701-13708	NN	denotes	complex
T4174	13684-13690	JJ	denotes	single
T4175	13691-13700	JJ	denotes	hexameric
T4176	13709-13711	IN	denotes	on
T4177	13712-13718	NN	denotes	bubble
T4178	13718-13719	HYPH	denotes	-
T4179	13719-13721	CD	denotes	20
T4180	13722-13723	-LRB-	denotes	(
T4181	13723-13724	DT	denotes	a
T4182	13732-13741	NN	denotes	substrate
T4183	13725-13731	NN	denotes	bubble
T4184	13742-13746	IN	denotes	with
T4185	13747-13749	CD	denotes	20
T4186	13750-13752	NN	denotes	nt
T4187	13753-13757	JJ	denotes	long
T4188	13782-13789	NN	denotes	segment
T4189	13758-13765	JJ	denotes	central
T4190	13766-13772	JJ	denotes	single
T4191	13773-13781	VBN	denotes	stranded
T4192	13772-13773	HYPH	denotes	-
T4193	13789-13790	-RRB-	denotes	)
T4194	13791-13794	CC	denotes	and
T4195	13795-13796	DT	denotes	a
T4196	13814-13821	NN	denotes	complex
T4197	13797-13803	JJ	denotes	double
T4198	13804-13813	JJ	denotes	hexameric
T4199	13822-13824	IN	denotes	on
T4200	13825-13831	NN	denotes	bubble
T4201	13835-13838	NN	denotes	DNA
T4202	13831-13832	HYPH	denotes	-
T4203	13832-13834	CD	denotes	60
T4204	13839-13840	-LRB-	denotes	(
T4205	13840-13842	CD	denotes	13
T4206	13842-13843	-RRB-	denotes	)
T4207	13843-13844	.	denotes	.
T4208	13844-14176	sentence	denotes	In order to determine the minimum requirement for double-hexamer formation, a set of synthetic bubble-like substrates (Bub-T10, Bub-T20, Bub-T30, Bub-T40 and Bub-T50) that differed in the length of the central unpaired segment were constructed, and the binding and helicase activities of Mcm4/6/7 on these substrates were examined.
T4209	13845-13847	IN	denotes	In
T4210	14077-14088	VBN	denotes	constructed
T4211	13848-13853	NN	denotes	order
T4212	13854-13856	TO	denotes	to
T4213	13857-13866	VB	denotes	determine
T4214	13867-13870	DT	denotes	the
T4215	13879-13890	NN	denotes	requirement
T4216	13871-13878	JJ	denotes	minimum
T4217	13891-13894	IN	denotes	for
T4218	13895-13901	JJ	denotes	double
T4219	13902-13909	NN	denotes	hexamer
T4220	13901-13902	HYPH	denotes	-
T4221	13910-13919	NN	denotes	formation
T4222	13919-13921	,	denotes	,
T4223	13921-13922	DT	denotes	a
T4224	13923-13926	NN	denotes	set
T4225	13927-13929	IN	denotes	of
T4226	13930-13939	JJ	denotes	synthetic
T4227	13952-13962	NNS	denotes	substrates
T4228	13940-13946	NN	denotes	bubble
T4229	13947-13951	JJ	denotes	like
T4230	13946-13947	HYPH	denotes	-
T4231	13963-13964	-LRB-	denotes	(
T4232	13964-13967	NN	denotes	Bub
T4233	13968-13971	NN	denotes	T10
T4234	13967-13968	HYPH	denotes	-
T4235	13971-13973	,	denotes	,
T4236	13973-13976	NN	denotes	Bub
T4237	13977-13980	NN	denotes	T20
T4238	13976-13977	HYPH	denotes	-
T4239	13980-13982	,	denotes	,
T4240	13982-13985	NN	denotes	Bub
T4241	13986-13989	NN	denotes	T30
T4242	13985-13986	HYPH	denotes	-
T4243	13989-13991	,	denotes	,
T4244	13991-13994	NN	denotes	Bub
T4245	13995-13998	NN	denotes	T40
T4246	13994-13995	HYPH	denotes	-
T4247	13999-14002	CC	denotes	and
T4248	14003-14006	NN	denotes	Bub
T4249	14007-14010	NN	denotes	T50
T4250	14006-14007	HYPH	denotes	-
T4251	14010-14011	-RRB-	denotes	)
T4252	14012-14016	WDT	denotes	that
T4253	14017-14025	VBD	denotes	differed
T4254	14026-14028	IN	denotes	in
T4255	14029-14032	DT	denotes	the
T4256	14033-14039	NN	denotes	length
T4257	14040-14042	IN	denotes	of
T4258	14043-14046	DT	denotes	the
T4259	14064-14071	NN	denotes	segment
T4260	14047-14054	JJ	denotes	central
T4261	14055-14063	JJ	denotes	unpaired
T4262	14072-14076	VBD	denotes	were
T4263	14088-14090	,	denotes	,
T4264	14090-14093	CC	denotes	and
T4265	14094-14097	DT	denotes	the
T4266	14119-14129	NNS	denotes	activities
T4267	14098-14105	NN	denotes	binding
T4268	14106-14109	CC	denotes	and
T4269	14110-14118	NN	denotes	helicase
T4270	14167-14175	VBN	denotes	examined
T4271	14130-14132	IN	denotes	of
T4272	14133-14137	NN	denotes	Mcm4
T4273	14137-14138	HYPH	denotes	/
T4274	14138-14139	CD	denotes	6
T4275	14139-14140	HYPH	denotes	/
T4276	14140-14141	CD	denotes	7
T4277	14142-14144	IN	denotes	on
T4278	14145-14150	DT	denotes	these
T4279	14151-14161	NNS	denotes	substrates
T4280	14162-14166	VBD	denotes	were
T4281	14175-14176	.	denotes	.
T4282	14176-14308	sentence	denotes	Mcm4/6/7 was incubated with a radiolabeled synthetic bubble substrate in the presence of 0.5 mM ATP-γ-S to allow complex formation.
T4283	14177-14181	NN	denotes	Mcm4
T4284	14190-14199	VBN	denotes	incubated
T4285	14181-14182	HYPH	denotes	/
T4286	14182-14183	CD	denotes	6
T4287	14183-14184	HYPH	denotes	/
T4288	14184-14185	CD	denotes	7
T4289	14186-14189	VBD	denotes	was
T4290	14200-14204	IN	denotes	with
T4291	14205-14206	DT	denotes	a
T4292	14237-14246	NN	denotes	substrate
T4293	14207-14219	VBN	denotes	radiolabeled
T4294	14220-14229	JJ	denotes	synthetic
T4295	14230-14236	NN	denotes	bubble
T4296	14247-14249	IN	denotes	in
T4297	14250-14253	DT	denotes	the
T4298	14254-14262	NN	denotes	presence
T4299	14263-14265	IN	denotes	of
T4300	14266-14269	CD	denotes	0.5
T4301	14270-14272	NN	denotes	mM
T4302	14279-14280	NN	denotes	S
T4303	14273-14276	NN	denotes	ATP
T4304	14276-14277	HYPH	denotes	-
T4305	14277-14278	SYM	denotes	γ
T4306	14278-14279	HYPH	denotes	-
T4307	14281-14283	TO	denotes	to
T4308	14284-14289	VB	denotes	allow
T4309	14290-14297	NN	denotes	complex
T4310	14298-14307	NN	denotes	formation
T4311	14307-14308	.	denotes	.
T4312	14308-14520	sentence	denotes	A half of these reaction mixtures were analyzed for DNA binding in gel shift assays (Figure 2A), and the remainder was further incubated in the presence of 10 mM ATP to measure DNA helicase activity (Figure 2B).
T4313	14309-14310	DT	denotes	A
T4314	14311-14315	NN	denotes	half
T4315	14348-14356	VBN	denotes	analyzed
T4316	14316-14318	IN	denotes	of
T4317	14319-14324	DT	denotes	these
T4318	14334-14342	NNS	denotes	mixtures
T4319	14325-14333	NN	denotes	reaction
T4320	14343-14347	VBD	denotes	were
T4321	14357-14360	IN	denotes	for
T4322	14361-14364	NN	denotes	DNA
T4323	14365-14372	NN	denotes	binding
T4324	14373-14375	IN	denotes	in
T4325	14376-14379	NN	denotes	gel
T4326	14380-14385	NN	denotes	shift
T4327	14386-14392	NNS	denotes	assays
T4328	14393-14394	-LRB-	denotes	(
T4329	14401-14403	NN	denotes	2A
T4330	14394-14400	NN	denotes	Figure
T4331	14403-14404	-RRB-	denotes	)
T4332	14404-14406	,	denotes	,
T4333	14406-14409	CC	denotes	and
T4334	14410-14413	DT	denotes	the
T4335	14414-14423	NN	denotes	remainder
T4336	14436-14445	VBN	denotes	incubated
T4337	14424-14427	VBD	denotes	was
T4338	14428-14435	RB	denotes	further
T4339	14446-14448	IN	denotes	in
T4340	14449-14452	DT	denotes	the
T4341	14453-14461	NN	denotes	presence
T4342	14462-14464	IN	denotes	of
T4343	14465-14467	CD	denotes	10
T4344	14468-14470	NN	denotes	mM
T4345	14471-14474	NN	denotes	ATP
T4346	14475-14477	TO	denotes	to
T4347	14478-14485	VB	denotes	measure
T4348	14486-14489	NN	denotes	DNA
T4349	14490-14498	NN	denotes	helicase
T4350	14499-14507	NN	denotes	activity
T4351	14508-14509	-LRB-	denotes	(
T4352	14516-14518	NN	denotes	2B
T4353	14509-14515	NN	denotes	Figure
T4354	14518-14519	-RRB-	denotes	)
T4355	14519-14520	.	denotes	.
T4356	14520-14693	sentence	denotes	Bub-10 generated only a low level of complexes with Mcm4/6/7, and Bub-20 generated primarily a single mobility-shifted form, which may contain a single hexamer of Mcm4/6/7.
T4357	14521-14524	NN	denotes	Bub
T4358	14528-14537	VBD	denotes	generated
T4359	14524-14525	HYPH	denotes	-
T4360	14525-14527	CD	denotes	10
T4361	14538-14542	RB	denotes	only
T4362	14549-14554	NN	denotes	level
T4363	14543-14544	DT	denotes	a
T4364	14545-14548	JJ	denotes	low
T4365	14555-14557	IN	denotes	of
T4366	14558-14567	NNS	denotes	complexes
T4367	14568-14572	IN	denotes	with
T4368	14573-14577	NN	denotes	Mcm4
T4369	14577-14578	HYPH	denotes	/
T4370	14578-14579	CD	denotes	6
T4371	14579-14580	HYPH	denotes	/
T4372	14580-14581	CD	denotes	7
T4373	14581-14583	,	denotes	,
T4374	14583-14586	CC	denotes	and
T4375	14587-14590	NN	denotes	Bub
T4376	14594-14603	VBD	denotes	generated
T4377	14590-14591	HYPH	denotes	-
T4378	14591-14593	CD	denotes	20
T4379	14604-14613	RB	denotes	primarily
T4380	14614-14615	DT	denotes	a
T4381	14640-14644	NN	denotes	form
T4382	14616-14622	JJ	denotes	single
T4383	14623-14631	NN	denotes	mobility
T4384	14632-14639	VBN	denotes	shifted
T4385	14631-14632	HYPH	denotes	-
T4386	14644-14646	,	denotes	,
T4387	14646-14651	WDT	denotes	which
T4388	14656-14663	VB	denotes	contain
T4389	14652-14655	MD	denotes	may
T4390	14664-14665	DT	denotes	a
T4391	14673-14680	NN	denotes	hexamer
T4392	14666-14672	JJ	denotes	single
T4393	14681-14683	IN	denotes	of
T4394	14684-14688	NN	denotes	Mcm4
T4395	14688-14689	HYPH	denotes	/
T4396	14689-14690	CD	denotes	6
T4397	14690-14691	HYPH	denotes	/
T4398	14691-14692	CD	denotes	7
T4399	14692-14693	.	denotes	.
T4400	14693-14849	sentence	denotes	On Bub-30, significant amount of complexes containing a double hexamer were generated in addition to those containing a single hexamer (Figure 2A) (13,21).
T4401	14694-14696	IN	denotes	On
T4402	14770-14779	VBN	denotes	generated
T4403	14697-14700	NN	denotes	Bub
T4404	14700-14701	HYPH	denotes	-
T4405	14701-14703	CD	denotes	30
T4406	14703-14705	,	denotes	,
T4407	14705-14716	JJ	denotes	significant
T4408	14717-14723	NN	denotes	amount
T4409	14724-14726	IN	denotes	of
T4410	14727-14736	NNS	denotes	complexes
T4411	14737-14747	VBG	denotes	containing
T4412	14748-14749	DT	denotes	a
T4413	14757-14764	NN	denotes	hexamer
T4414	14750-14756	JJ	denotes	double
T4415	14765-14769	VBD	denotes	were
T4416	14780-14782	IN	denotes	in
T4417	14783-14791	NN	denotes	addition
T4418	14792-14794	IN	denotes	to
T4419	14795-14800	DT	denotes	those
T4420	14801-14811	VBG	denotes	containing
T4421	14812-14813	DT	denotes	a
T4422	14821-14828	NN	denotes	hexamer
T4423	14814-14820	JJ	denotes	single
T4424	14829-14830	-LRB-	denotes	(
T4425	14837-14839	NN	denotes	2A
T4426	14830-14836	NN	denotes	Figure
T4427	14839-14840	-RRB-	denotes	)
T4428	14841-14842	-LRB-	denotes	(
T4429	14845-14847	CD	denotes	21
T4430	14842-14844	CD	denotes	13
T4431	14844-14845	,	denotes	,
T4432	14847-14848	-RRB-	denotes	)
T4433	14848-14849	.	denotes	.
T4434	14849-14989	sentence	denotes	The amounts of double hexamer complexes increased on Bub-T40 and Bub-T50 with concomitant decrease of single hexamer complexes (Figure 2A).
T4435	14850-14853	DT	denotes	The
T4436	14854-14861	NNS	denotes	amounts
T4437	14890-14899	VBD	denotes	increased
T4438	14862-14864	IN	denotes	of
T4439	14865-14871	JJ	denotes	double
T4440	14872-14879	NN	denotes	hexamer
T4441	14880-14889	NNS	denotes	complexes
T4442	14900-14902	IN	denotes	on
T4443	14903-14906	NN	denotes	Bub
T4444	14907-14910	NN	denotes	T40
T4445	14906-14907	HYPH	denotes	-
T4446	14911-14914	CC	denotes	and
T4447	14915-14918	NN	denotes	Bub
T4448	14919-14922	NN	denotes	T50
T4449	14918-14919	HYPH	denotes	-
T4450	14923-14927	IN	denotes	with
T4451	14928-14939	JJ	denotes	concomitant
T4452	14940-14948	NN	denotes	decrease
T4453	14949-14951	IN	denotes	of
T4454	14952-14958	JJ	denotes	single
T4455	14959-14966	NN	denotes	hexamer
T4456	14967-14976	NNS	denotes	complexes
T4457	14977-14978	-LRB-	denotes	(
T4458	14985-14987	NN	denotes	2A
T4459	14978-14984	NN	denotes	Figure
T4460	14987-14988	-RRB-	denotes	)
T4461	14988-14989	.	denotes	.
T4462	14989-15158	sentence	denotes	The results indicate that a single-stranded segment of at least 40 nt long is required for efficient loading of the double-hexameric Mcm helicase on a bubble substrate.
T4463	14990-14993	DT	denotes	The
T4464	14994-15001	NNS	denotes	results
T4465	15002-15010	VBP	denotes	indicate
T4466	15011-15015	IN	denotes	that
T4467	15068-15076	VBN	denotes	required
T4468	15016-15017	DT	denotes	a
T4469	15034-15041	NN	denotes	segment
T4470	15018-15024	JJ	denotes	single
T4471	15025-15033	VBN	denotes	stranded
T4472	15024-15025	HYPH	denotes	-
T4473	15042-15044	IN	denotes	of
T4474	15045-15047	RB	denotes	at
T4475	15048-15053	RBS	denotes	least
T4476	15054-15056	CD	denotes	40
T4477	15057-15059	NN	denotes	nt
T4478	15060-15064	JJ	denotes	long
T4479	15065-15067	VBZ	denotes	is
T4480	15077-15080	IN	denotes	for
T4481	15081-15090	JJ	denotes	efficient
T4482	15091-15098	NN	denotes	loading
T4483	15099-15101	IN	denotes	of
T4484	15102-15105	DT	denotes	the
T4485	15127-15135	NN	denotes	helicase
T4486	15106-15112	JJ	denotes	double
T4487	15113-15122	JJ	denotes	hexameric
T4488	15112-15113	HYPH	denotes	-
T4489	15123-15126	NN	denotes	Mcm
T4490	15136-15138	IN	denotes	on
T4491	15139-15140	DT	denotes	a
T4492	15148-15157	NN	denotes	substrate
T4493	15141-15147	NN	denotes	bubble
T4494	15157-15158	.	denotes	.
T4495	15158-15268	sentence	denotes	In DNA helicase assays, Mcm4/6/7 complex did not show helicase activity on either Bub-10 or Bub-20 substrate.
T4496	15159-15161	IN	denotes	In
T4497	15208-15212	VB	denotes	show
T4498	15162-15165	NN	denotes	DNA
T4499	15166-15174	NN	denotes	helicase
T4500	15175-15181	NNS	denotes	assays
T4501	15181-15183	,	denotes	,
T4502	15183-15187	NN	denotes	Mcm4
T4503	15192-15199	NN	denotes	complex
T4504	15187-15188	HYPH	denotes	/
T4505	15188-15189	CD	denotes	6
T4506	15189-15190	HYPH	denotes	/
T4507	15190-15191	CD	denotes	7
T4508	15200-15203	VBD	denotes	did
T4509	15204-15207	RB	denotes	not
T4510	15213-15221	NN	denotes	helicase
T4511	15222-15230	NN	denotes	activity
T4512	15231-15233	IN	denotes	on
T4513	15234-15240	CC	denotes	either
T4514	15241-15244	NN	denotes	Bub
T4515	15258-15267	NN	denotes	substrate
T4516	15244-15245	HYPH	denotes	-
T4517	15245-15247	CD	denotes	10
T4518	15248-15250	CC	denotes	or
T4519	15251-15254	NN	denotes	Bub
T4520	15254-15255	HYPH	denotes	-
T4521	15255-15257	CD	denotes	20
T4522	15267-15268	.	denotes	.
T4523	15268-15385	sentence	denotes	The efficiency of displacement slightly improved as the length of the single-stranded segment increased up to 30 nt.
T4524	15269-15272	DT	denotes	The
T4525	15273-15283	NN	denotes	efficiency
T4526	15309-15317	VBD	denotes	improved
T4527	15284-15286	IN	denotes	of
T4528	15287-15299	NN	denotes	displacement
T4529	15300-15308	RB	denotes	slightly
T4530	15318-15320	IN	denotes	as
T4531	15363-15372	VBD	denotes	increased
T4532	15321-15324	DT	denotes	the
T4533	15325-15331	NN	denotes	length
T4534	15332-15334	IN	denotes	of
T4535	15335-15338	DT	denotes	the
T4536	15355-15362	NN	denotes	segment
T4537	15339-15345	JJ	denotes	single
T4538	15346-15354	VBN	denotes	stranded
T4539	15345-15346	HYPH	denotes	-
T4540	15373-15375	IN	denotes	up
T4541	15379-15381	CD	denotes	30
T4542	15376-15378	IN	denotes	to
T4543	15382-15384	NN	denotes	nt
T4544	15384-15385	.	denotes	.
T4545	15385-15609	sentence	denotes	On Bub-T40 and Bub-T50, on the other hand, significant displacement was observed, indicating that a single-stranded segment of at least 40 nt long is required for efficient helicase actions on bubble substrates (Figure 2B).
T4546	15386-15388	IN	denotes	On
T4547	15458-15466	VBN	denotes	observed
T4548	15389-15392	NN	denotes	Bub
T4549	15393-15396	NN	denotes	T40
T4550	15392-15393	HYPH	denotes	-
T4551	15397-15400	CC	denotes	and
T4552	15401-15404	NN	denotes	Bub
T4553	15405-15408	NN	denotes	T50
T4554	15404-15405	HYPH	denotes	-
T4555	15408-15410	,	denotes	,
T4556	15410-15412	IN	denotes	on
T4557	15413-15416	DT	denotes	the
T4558	15423-15427	NN	denotes	hand
T4559	15417-15422	JJ	denotes	other
T4560	15427-15429	,	denotes	,
T4561	15429-15440	JJ	denotes	significant
T4562	15441-15453	NN	denotes	displacement
T4563	15454-15457	VBD	denotes	was
T4564	15466-15468	,	denotes	,
T4565	15468-15478	VBG	denotes	indicating
T4566	15479-15483	IN	denotes	that
T4567	15536-15544	VBN	denotes	required
T4568	15484-15485	DT	denotes	a
T4569	15502-15509	NN	denotes	segment
T4570	15486-15492	JJ	denotes	single
T4571	15493-15501	VBN	denotes	stranded
T4572	15492-15493	HYPH	denotes	-
T4573	15510-15512	IN	denotes	of
T4574	15513-15515	RB	denotes	at
T4575	15516-15521	RBS	denotes	least
T4576	15522-15524	CD	denotes	40
T4577	15525-15527	NN	denotes	nt
T4578	15528-15532	JJ	denotes	long
T4579	15533-15535	VBZ	denotes	is
T4580	15545-15548	IN	denotes	for
T4581	15549-15558	JJ	denotes	efficient
T4582	15568-15575	NNS	denotes	actions
T4583	15559-15567	NN	denotes	helicase
T4584	15576-15578	IN	denotes	on
T4585	15579-15585	NN	denotes	bubble
T4586	15586-15596	NNS	denotes	substrates
T4587	15597-15598	-LRB-	denotes	(
T4588	15605-15607	NN	denotes	2B
T4589	15598-15604	NN	denotes	Figure
T4590	15607-15608	-RRB-	denotes	)
T4591	15608-15609	.	denotes	.
T4592	15609-15808	sentence	denotes	Taken together, the above results are consistent with the notion that the Mcm4/6/7 complex, loaded onto DNA as a double hexameric complex, efficiently unwinds the duplex region of the substrate DNA.
T4593	15610-15615	VBN	denotes	Taken
T4594	15644-15647	VBP	denotes	are
T4595	15616-15624	RB	denotes	together
T4596	15624-15626	,	denotes	,
T4597	15626-15629	DT	denotes	the
T4598	15636-15643	NNS	denotes	results
T4599	15630-15635	JJ	denotes	above
T4600	15648-15658	JJ	denotes	consistent
T4601	15659-15663	IN	denotes	with
T4602	15664-15667	DT	denotes	the
T4603	15668-15674	NN	denotes	notion
T4604	15675-15679	IN	denotes	that
T4605	15761-15768	VBZ	denotes	unwinds
T4606	15680-15683	DT	denotes	the
T4607	15693-15700	NN	denotes	complex
T4608	15684-15688	NN	denotes	Mcm4
T4609	15688-15689	HYPH	denotes	/
T4610	15689-15690	CD	denotes	6
T4611	15690-15691	HYPH	denotes	/
T4612	15691-15692	CD	denotes	7
T4613	15700-15702	,	denotes	,
T4614	15702-15708	VBN	denotes	loaded
T4615	15709-15713	IN	denotes	onto
T4616	15714-15717	NN	denotes	DNA
T4617	15718-15720	IN	denotes	as
T4618	15721-15722	DT	denotes	a
T4619	15740-15747	NN	denotes	complex
T4620	15723-15729	JJ	denotes	double
T4621	15730-15739	JJ	denotes	hexameric
T4622	15747-15749	,	denotes	,
T4623	15749-15760	RB	denotes	efficiently
T4624	15769-15772	DT	denotes	the
T4625	15780-15786	NN	denotes	region
T4626	15773-15779	NN	denotes	duplex
T4627	15787-15789	IN	denotes	of
T4628	15790-15793	DT	denotes	the
T4629	15804-15807	NN	denotes	DNA
T4630	15794-15803	NN	denotes	substrate
T4631	15807-15808	.	denotes	.
T4737	15810-15814	DT	denotes	Both
T4738	15815-15822	NNS	denotes	strands
T4739	15868-15876	VBN	denotes	required
T4740	15823-15825	IN	denotes	of
T4741	15826-15833	NN	denotes	thymine
T4742	15834-15838	JJ	denotes	rich
T4743	15833-15834	HYPH	denotes	-
T4744	15855-15863	NNS	denotes	segments
T4745	15839-15845	JJ	denotes	single
T4746	15846-15854	VBN	denotes	stranded
T4747	15845-15846	HYPH	denotes	-
T4748	15864-15867	VBP	denotes	are
T4749	15877-15880	IN	denotes	for
T4750	15881-15890	JJ	denotes	efficient
T4751	15891-15900	NN	denotes	unwinding
T4752	15901-15903	IN	denotes	of
T4753	15904-15910	NN	denotes	bubble
T4754	15911-15914	NN	denotes	DNA
T4755	15915-15917	IN	denotes	by
T4756	15918-15922	NN	denotes	Mcm4
T4757	15922-15923	HYPH	denotes	/
T4758	15923-15924	CD	denotes	6
T4759	15924-15925	HYPH	denotes	/
T4760	15925-15926	CD	denotes	7
T4761	15926-16067	sentence	denotes	Symmetric binding of the Mcm4/6/7 double hexamer to bubble suggests that each hexamer catalyzes unwinding of one of the two duplex segments.
T4762	15927-15936	JJ	denotes	Symmetric
T4763	15937-15944	NN	denotes	binding
T4764	15986-15994	VBZ	denotes	suggests
T4765	15945-15947	IN	denotes	of
T4766	15948-15951	DT	denotes	the
T4767	15968-15975	NN	denotes	hexamer
T4768	15952-15956	NN	denotes	Mcm4
T4769	15956-15957	HYPH	denotes	/
T4770	15957-15958	CD	denotes	6
T4771	15958-15959	HYPH	denotes	/
T4772	15959-15960	CD	denotes	7
T4773	15961-15967	JJ	denotes	double
T4774	15976-15978	IN	denotes	to
T4775	15979-15985	NN	denotes	bubble
T4776	15995-15999	IN	denotes	that
T4777	16013-16022	VBZ	denotes	catalyzes
T4778	16000-16004	DT	denotes	each
T4779	16005-16012	NN	denotes	hexamer
T4780	16023-16032	NN	denotes	unwinding
T4781	16033-16035	IN	denotes	of
T4782	16036-16039	CD	denotes	one
T4783	16040-16042	IN	denotes	of
T4784	16043-16046	DT	denotes	the
T4785	16058-16066	NNS	denotes	segments
T4786	16047-16050	CD	denotes	two
T4787	16051-16057	NN	denotes	duplex
T4788	16066-16067	.	denotes	.
T4789	16067-16163	sentence	denotes	We asked whether activation of both hexamers is required for unwinding of the bubble substrate.
T4790	16068-16070	PRP	denotes	We
T4791	16071-16076	VBD	denotes	asked
T4792	16077-16084	IN	denotes	whether
T4793	16116-16124	VBN	denotes	required
T4794	16085-16095	NN	denotes	activation
T4795	16096-16098	IN	denotes	of
T4796	16099-16103	DT	denotes	both
T4797	16104-16112	NNS	denotes	hexamers
T4798	16113-16115	VBZ	denotes	is
T4799	16125-16128	IN	denotes	for
T4800	16129-16138	NN	denotes	unwinding
T4801	16139-16141	IN	denotes	of
T4802	16142-16145	DT	denotes	the
T4803	16153-16162	NN	denotes	substrate
T4804	16146-16152	NN	denotes	bubble
T4805	16162-16163	.	denotes	.
T4806	16163-16310	sentence	denotes	The preferred bubble substrate which is efficiently unwound by Mcm4/6/7 contains thymine stretches on both strands of the single-stranded segment.
T4807	16164-16167	DT	denotes	The
T4808	16185-16194	NN	denotes	substrate
T4809	16168-16177	JJ	denotes	preferred
T4810	16178-16184	NN	denotes	bubble
T4811	16236-16244	VBZ	denotes	contains
T4812	16195-16200	WDT	denotes	which
T4813	16216-16223	VBN	denotes	unwound
T4814	16201-16203	VBZ	denotes	is
T4815	16204-16215	RB	denotes	efficiently
T4816	16224-16226	IN	denotes	by
T4817	16227-16231	NN	denotes	Mcm4
T4818	16231-16232	HYPH	denotes	/
T4819	16232-16233	CD	denotes	6
T4820	16233-16234	HYPH	denotes	/
T4821	16234-16235	CD	denotes	7
T4822	16245-16252	NN	denotes	thymine
T4823	16253-16262	NNS	denotes	stretches
T4824	16263-16265	IN	denotes	on
T4825	16266-16270	DT	denotes	both
T4826	16271-16278	NNS	denotes	strands
T4827	16279-16281	IN	denotes	of
T4828	16282-16285	DT	denotes	the
T4829	16302-16309	NN	denotes	segment
T4830	16286-16292	JJ	denotes	single
T4831	16293-16301	VBN	denotes	stranded
T4832	16292-16293	HYPH	denotes	-
T4833	16309-16310	.	denotes	.
T4834	16310-16542	sentence	denotes	We constructed a hetero-bubble (Bub66/T–G-rich) in which one strand is T-rich (same as Bub66/T-rich) and the other strand is G-rich (same as Bub66/G-rich) (13), and helicase assays were conducted with increasing amount of Mcm4/6/7.
T4835	16311-16313	PRP	denotes	We
T4836	16314-16325	VBD	denotes	constructed
T4837	16326-16327	DT	denotes	a
T4838	16328-16341	JJ	denotes	hetero-bubble
T4839	16342-16343	-LRB-	denotes	(
T4840	16343-16348	NN	denotes	Bub66
T4841	16351-16352	NN	denotes	G
T4842	16348-16349	HYPH	denotes	/
T4843	16349-16350	NN	denotes	T
T4844	16350-16351	HYPH	denotes	–
T4845	16352-16353	HYPH	denotes	-
T4846	16353-16357	JJ	denotes	rich
T4847	16357-16358	-RRB-	denotes	)
T4848	16359-16361	IN	denotes	in
T4849	16379-16381	VBZ	denotes	is
T4850	16362-16367	WDT	denotes	which
T4851	16368-16371	CD	denotes	one
T4852	16372-16378	NN	denotes	strand
T4853	16382-16383	NN	denotes	T
T4854	16384-16388	JJ	denotes	rich
T4855	16383-16384	HYPH	denotes	-
T4856	16389-16390	-LRB-	denotes	(
T4857	16390-16394	JJ	denotes	same
T4858	16395-16397	IN	denotes	as
T4859	16398-16403	NN	denotes	Bub66
T4860	16404-16405	NN	denotes	T
T4861	16403-16404	HYPH	denotes	/
T4862	16405-16406	HYPH	denotes	-
T4863	16406-16410	JJ	denotes	rich
T4864	16410-16411	-RRB-	denotes	)
T4865	16412-16415	CC	denotes	and
T4866	16416-16419	DT	denotes	the
T4867	16426-16432	NN	denotes	strand
T4868	16420-16425	JJ	denotes	other
T4869	16433-16435	VBZ	denotes	is
T4870	16436-16437	NN	denotes	G
T4871	16438-16442	JJ	denotes	rich
T4872	16437-16438	HYPH	denotes	-
T4873	16443-16444	-LRB-	denotes	(
T4874	16444-16448	JJ	denotes	same
T4875	16449-16451	IN	denotes	as
T4876	16452-16457	NN	denotes	Bub66
T4877	16458-16459	NN	denotes	G
T4878	16457-16458	HYPH	denotes	/
T4879	16459-16460	HYPH	denotes	-
T4880	16460-16464	JJ	denotes	rich
T4881	16464-16465	-RRB-	denotes	)
T4882	16466-16467	-LRB-	denotes	(
T4883	16467-16469	CD	denotes	13
T4884	16469-16470	-RRB-	denotes	)
T4885	16470-16472	,	denotes	,
T4886	16472-16475	CC	denotes	and
T4887	16476-16484	NN	denotes	helicase
T4888	16485-16491	NNS	denotes	assays
T4889	16497-16506	VBN	denotes	conducted
T4890	16492-16496	VBD	denotes	were
T4891	16507-16511	IN	denotes	with
T4892	16512-16522	VBG	denotes	increasing
T4893	16523-16529	NN	denotes	amount
T4894	16530-16532	IN	denotes	of
T4895	16533-16537	NN	denotes	Mcm4
T4896	16537-16538	HYPH	denotes	/
T4897	16538-16539	CD	denotes	6
T4898	16539-16540	HYPH	denotes	/
T4899	16540-16541	CD	denotes	7
T4900	16541-16542	.	denotes	.
T4901	16542-16631	sentence	denotes	Only weak unwinding was detected on this substrate compared to Bub66/T-rich (Figure 3A).
T4902	16543-16547	RB	denotes	Only
T4903	16553-16562	NN	denotes	unwinding
T4904	16548-16552	JJ	denotes	weak
T4905	16567-16575	VBN	denotes	detected
T4906	16563-16566	VBD	denotes	was
T4907	16576-16578	IN	denotes	on
T4908	16579-16583	DT	denotes	this
T4909	16584-16593	NN	denotes	substrate
T4910	16594-16602	VBN	denotes	compared
T4911	16603-16605	IN	denotes	to
T4912	16606-16611	NN	denotes	Bub66
T4913	16612-16613	NN	denotes	T
T4914	16611-16612	HYPH	denotes	/
T4915	16613-16614	HYPH	denotes	-
T4916	16614-16618	JJ	denotes	rich
T4917	16619-16620	-LRB-	denotes	(
T4918	16627-16629	NN	denotes	3A
T4919	16620-16626	NN	denotes	Figure
T4920	16629-16630	-RRB-	denotes	)
T4921	16630-16631	.	denotes	.
T4922	16631-16822	sentence	denotes	Since Mcm4/6/7 can bind to Bub66/T–G-rich and forms a double hexameric complex (data not shown), the defective unwinding is not due to inefficient loading of the helicase onto the substrate.
T4923	16632-16637	IN	denotes	Since
T4924	16651-16655	VB	denotes	bind
T4925	16638-16642	NN	denotes	Mcm4
T4926	16642-16643	HYPH	denotes	/
T4927	16643-16644	CD	denotes	6
T4928	16644-16645	HYPH	denotes	/
T4929	16645-16646	CD	denotes	7
T4930	16647-16650	MD	denotes	can
T4931	16753-16755	VBZ	denotes	is
T4932	16656-16658	IN	denotes	to
T4933	16659-16664	NN	denotes	Bub66
T4934	16667-16668	NN	denotes	G
T4935	16664-16665	HYPH	denotes	/
T4936	16665-16666	NN	denotes	T
T4937	16666-16667	HYPH	denotes	–
T4938	16668-16669	HYPH	denotes	-
T4939	16669-16673	JJ	denotes	rich
T4940	16674-16677	CC	denotes	and
T4941	16678-16683	VBZ	denotes	forms
T4942	16684-16685	DT	denotes	a
T4943	16703-16710	NN	denotes	complex
T4944	16686-16692	JJ	denotes	double
T4945	16693-16702	JJ	denotes	hexameric
T4946	16711-16712	-LRB-	denotes	(
T4947	16721-16726	VBN	denotes	shown
T4948	16712-16716	NNS	denotes	data
T4949	16717-16720	RB	denotes	not
T4950	16726-16727	-RRB-	denotes	)
T4951	16727-16729	,	denotes	,
T4952	16729-16732	DT	denotes	the
T4953	16743-16752	NN	denotes	unwinding
T4954	16733-16742	JJ	denotes	defective
T4955	16756-16759	RB	denotes	not
T4956	16760-16763	IN	denotes	due
T4957	16764-16766	IN	denotes	to
T4958	16767-16778	JJ	denotes	inefficient
T4959	16779-16786	NN	denotes	loading
T4960	16787-16789	IN	denotes	of
T4961	16790-16793	DT	denotes	the
T4962	16794-16802	NN	denotes	helicase
T4963	16803-16807	IN	denotes	onto
T4964	16808-16811	DT	denotes	the
T4965	16812-16821	NN	denotes	substrate
T4966	16821-16822	.	denotes	.
T4967	16822-17120	sentence	denotes	If one hexamer interacting with the thymine-rich strand dissociates from the other hexamer and displaces only one of the duplex segments, then it would result in generation of Y-fork structures which could not be further displaced due to the presence of G-rich 3′-tail and would appear on the gel.
T4968	16823-16825	IN	denotes	If
T4969	16879-16890	VBZ	denotes	dissociates
T4970	16826-16829	CD	denotes	one
T4971	16830-16837	NN	denotes	hexamer
T4972	16838-16849	VBG	denotes	interacting
T4973	16850-16854	IN	denotes	with
T4974	16855-16858	DT	denotes	the
T4975	16872-16878	NN	denotes	strand
T4976	16859-16866	NN	denotes	thymine
T4977	16867-16871	JJ	denotes	rich
T4978	16866-16867	HYPH	denotes	-
T4979	16975-16981	VB	denotes	result
T4980	16891-16895	IN	denotes	from
T4981	16896-16899	DT	denotes	the
T4982	16906-16913	NN	denotes	hexamer
T4983	16900-16905	JJ	denotes	other
T4984	16914-16917	CC	denotes	and
T4985	16918-16927	VBZ	denotes	displaces
T4986	16928-16932	RB	denotes	only
T4987	16933-16936	CD	denotes	one
T4988	16937-16939	IN	denotes	of
T4989	16940-16943	DT	denotes	the
T4990	16951-16959	NNS	denotes	segments
T4991	16944-16950	NN	denotes	duplex
T4992	16959-16961	,	denotes	,
T4993	16961-16965	RB	denotes	then
T4994	16966-16968	PRP	denotes	it
T4995	16969-16974	MD	denotes	would
T4996	16982-16984	IN	denotes	in
T4997	16985-16995	NN	denotes	generation
T4998	16996-16998	IN	denotes	of
T4999	16999-17000	NN	denotes	Y
T5000	17001-17005	NN	denotes	fork
T5001	17000-17001	HYPH	denotes	-
T5002	17006-17016	NNS	denotes	structures
T5003	17017-17022	WDT	denotes	which
T5004	17044-17053	VBN	denotes	displaced
T5005	17023-17028	MD	denotes	could
T5006	17029-17032	RB	denotes	not
T5007	17033-17035	VB	denotes	be
T5008	17036-17043	RB	denotes	further
T5009	17054-17057	IN	denotes	due
T5010	17058-17060	IN	denotes	to
T5011	17061-17064	DT	denotes	the
T5012	17065-17073	NN	denotes	presence
T5013	17074-17076	IN	denotes	of
T5014	17077-17078	NN	denotes	G
T5015	17079-17083	JJ	denotes	rich
T5016	17078-17079	HYPH	denotes	-
T5017	17087-17091	NN	denotes	tail
T5018	17084-17085	CD	denotes	3
T5019	17085-17086	SYM	denotes	′
T5020	17086-17087	HYPH	denotes	-
T5021	17092-17095	CC	denotes	and
T5022	17096-17101	MD	denotes	would
T5023	17102-17108	VB	denotes	appear
T5024	17109-17111	IN	denotes	on
T5025	17112-17115	DT	denotes	the
T5026	17116-17119	NN	denotes	gel
T5027	17119-17120	.	denotes	.
T5028	17120-17346	sentence	denotes	However, we did not detect such intermediates during the course of the experiment (Figure 3B), suggesting that bubble can be displaced efficiently only when two molecules of the Mcm4/6/7 hexamers are simultaneously activated.
T5029	17121-17128	RB	denotes	However
T5030	17141-17147	VB	denotes	detect
T5031	17128-17130	,	denotes	,
T5032	17130-17132	PRP	denotes	we
T5033	17133-17136	VBD	denotes	did
T5034	17137-17140	RB	denotes	not
T5035	17148-17152	JJ	denotes	such
T5036	17153-17166	NNS	denotes	intermediates
T5037	17167-17173	IN	denotes	during
T5038	17174-17177	DT	denotes	the
T5039	17178-17184	NN	denotes	course
T5040	17185-17187	IN	denotes	of
T5041	17188-17191	DT	denotes	the
T5042	17192-17202	NN	denotes	experiment
T5043	17203-17204	-LRB-	denotes	(
T5044	17211-17213	NN	denotes	3B
T5045	17204-17210	NN	denotes	Figure
T5046	17213-17214	-RRB-	denotes	)
T5047	17214-17216	,	denotes	,
T5048	17216-17226	VBG	denotes	suggesting
T5049	17227-17231	IN	denotes	that
T5050	17246-17255	VBN	denotes	displaced
T5051	17232-17238	NN	denotes	bubble
T5052	17239-17242	MD	denotes	can
T5053	17243-17245	VB	denotes	be
T5054	17256-17267	RB	denotes	efficiently
T5055	17268-17272	RB	denotes	only
T5056	17336-17345	VBN	denotes	activated
T5057	17273-17277	WRB	denotes	when
T5058	17278-17281	CD	denotes	two
T5059	17282-17291	NNS	denotes	molecules
T5060	17292-17294	IN	denotes	of
T5061	17295-17298	DT	denotes	the
T5062	17308-17316	NNS	denotes	hexamers
T5063	17299-17303	NN	denotes	Mcm4
T5064	17303-17304	HYPH	denotes	/
T5065	17304-17305	CD	denotes	6
T5066	17305-17306	HYPH	denotes	/
T5067	17306-17307	CD	denotes	7
T5068	17317-17320	VBP	denotes	are
T5069	17321-17335	RB	denotes	simultaneously
T5070	17345-17346	.	denotes	.
T5260	17348-17355	NN	denotes	Binding
T5261	17369-17376	NNS	denotes	actions
T5262	17356-17359	CC	denotes	and
T5263	17360-17368	NN	denotes	helicase
T5264	17377-17379	IN	denotes	of
T5265	17380-17384	NN	denotes	Mcm4
T5266	17384-17385	HYPH	denotes	/
T5267	17385-17386	CD	denotes	6
T5268	17386-17387	HYPH	denotes	/
T5269	17387-17388	CD	denotes	7
T5270	17389-17391	IN	denotes	on
T5271	17392-17398	VBN	denotes	forked
T5272	17423-17433	NNS	denotes	substrates
T5273	17399-17402	CC	denotes	and
T5274	17403-17404	CD	denotes	3
T5275	17413-17422	NN	denotes	extension
T5276	17404-17405	SYM	denotes	′
T5277	17405-17406	HYPH	denotes	-
T5278	17407-17409	CC	denotes	or
T5279	17410-17411	CD	denotes	5
T5280	17411-17412	SYM	denotes	′
T5281	17412-17413	HYPH	denotes	-
T5282	17433-17736	sentence	denotes	In order to examine more precisely the interaction and helicase actions with forked substrates, Y-fork substrates containing a nascent leading strand (A-fork[3′]), nascent lagging strand (A-fork[5′]), or both of them (A-fork[3′,5′]), as well as 5′-extension or 3′-extension structures were constructed.
T5283	17434-17436	IN	denotes	In
T5284	17724-17735	VBN	denotes	constructed
T5285	17437-17442	NN	denotes	order
T5286	17443-17445	TO	denotes	to
T5287	17446-17453	VB	denotes	examine
T5288	17454-17458	RBR	denotes	more
T5289	17459-17468	RB	denotes	precisely
T5290	17469-17472	DT	denotes	the
T5291	17473-17484	NN	denotes	interaction
T5292	17485-17488	CC	denotes	and
T5293	17489-17497	NN	denotes	helicase
T5294	17498-17505	NNS	denotes	actions
T5295	17506-17510	IN	denotes	with
T5296	17511-17517	VBN	denotes	forked
T5297	17518-17528	NNS	denotes	substrates
T5298	17528-17530	,	denotes	,
T5299	17530-17531	NN	denotes	Y
T5300	17532-17536	NN	denotes	fork
T5301	17531-17532	HYPH	denotes	-
T5302	17537-17547	NNS	denotes	substrates
T5303	17548-17558	VBG	denotes	containing
T5304	17559-17560	DT	denotes	a
T5305	17577-17583	NN	denotes	strand
T5306	17561-17568	JJ	denotes	nascent
T5307	17569-17576	VBG	denotes	leading
T5308	17584-17585	-LRB-	denotes	(
T5309	17585-17586	NN	denotes	A
T5310	17587-17591	NN	denotes	fork
T5311	17586-17587	HYPH	denotes	-
T5312	17591-17592	-LRB-	denotes	[
T5313	17592-17593	CD	denotes	3
T5314	17593-17594	SYM	denotes	′
T5315	17594-17595	-RRB-	denotes	]
T5316	17595-17596	-RRB-	denotes	)
T5317	17596-17598	,	denotes	,
T5318	17598-17605	JJ	denotes	nascent
T5319	17614-17620	NN	denotes	strand
T5320	17606-17613	VBG	denotes	lagging
T5321	17621-17622	-LRB-	denotes	(
T5322	17622-17623	NN	denotes	A
T5323	17624-17628	NN	denotes	fork
T5324	17623-17624	HYPH	denotes	-
T5325	17628-17629	-LRB-	denotes	[
T5326	17629-17630	CD	denotes	5
T5327	17630-17631	SYM	denotes	′
T5328	17631-17632	-RRB-	denotes	]
T5329	17632-17633	-RRB-	denotes	)
T5330	17633-17635	,	denotes	,
T5331	17635-17637	CC	denotes	or
T5332	17638-17642	DT	denotes	both
T5333	17643-17645	IN	denotes	of
T5334	17646-17650	PRP	denotes	them
T5335	17651-17652	-LRB-	denotes	(
T5336	17652-17653	NN	denotes	A
T5337	17654-17658	NN	denotes	fork
T5338	17653-17654	HYPH	denotes	-
T5339	17708-17718	NNS	denotes	structures
T5340	17658-17659	-LRB-	denotes	[
T5341	17659-17660	CD	denotes	3
T5342	17660-17661	SYM	denotes	′
T5343	17661-17662	,	denotes	,
T5344	17662-17663	CD	denotes	5
T5345	17663-17664	SYM	denotes	′
T5346	17664-17665	-RRB-	denotes	]
T5347	17665-17666	-RRB-	denotes	)
T5348	17666-17668	,	denotes	,
T5349	17668-17670	RB	denotes	as
T5350	17676-17678	IN	denotes	as
T5351	17671-17675	RB	denotes	well
T5352	17679-17680	CD	denotes	5
T5353	17682-17691	NN	denotes	extension
T5354	17680-17681	SYM	denotes	′
T5355	17681-17682	HYPH	denotes	-
T5356	17692-17694	CC	denotes	or
T5357	17695-17696	CD	denotes	3
T5358	17698-17707	NN	denotes	extension
T5359	17696-17697	SYM	denotes	′
T5360	17697-17698	HYPH	denotes	-
T5361	17719-17723	VBD	denotes	were
T5362	17735-17736	.	denotes	.
T5363	17736-17860	sentence	denotes	Mcm4/6/7 bound to A-fork[3′] or A-fork[5′] with affinity similar to that of a simple Y-fork (Figure 4A and data not shown).
T5364	17737-17741	NN	denotes	Mcm4
T5365	17746-17751	VBD	denotes	bound
T5366	17741-17742	HYPH	denotes	/
T5367	17742-17743	CD	denotes	6
T5368	17743-17744	HYPH	denotes	/
T5369	17744-17745	CD	denotes	7
T5370	17752-17754	IN	denotes	to
T5371	17755-17756	NN	denotes	A
T5372	17757-17761	NN	denotes	fork
T5373	17756-17757	HYPH	denotes	-
T5374	17761-17762	-LRB-	denotes	[
T5375	17762-17763	CD	denotes	3
T5376	17763-17764	SYM	denotes	′
T5377	17764-17765	-RRB-	denotes	]
T5378	17766-17768	CC	denotes	or
T5379	17769-17770	NN	denotes	A
T5380	17771-17775	NN	denotes	fork
T5381	17770-17771	HYPH	denotes	-
T5382	17775-17776	-LRB-	denotes	[
T5383	17776-17777	CD	denotes	5
T5384	17777-17778	SYM	denotes	′
T5385	17778-17779	-RRB-	denotes	]
T5386	17780-17784	IN	denotes	with
T5387	17785-17793	NN	denotes	affinity
T5388	17794-17801	JJ	denotes	similar
T5389	17802-17804	IN	denotes	to
T5390	17805-17809	DT	denotes	that
T5391	17810-17812	IN	denotes	of
T5392	17813-17814	DT	denotes	a
T5393	17824-17828	NN	denotes	fork
T5394	17815-17821	JJ	denotes	simple
T5395	17822-17823	NN	denotes	Y
T5396	17823-17824	HYPH	denotes	-
T5397	17829-17830	-LRB-	denotes	(
T5398	17837-17839	NN	denotes	4A
T5399	17830-17836	NN	denotes	Figure
T5400	17840-17843	CC	denotes	and
T5401	17844-17848	NNS	denotes	data
T5402	17853-17858	VBN	denotes	shown
T5403	17849-17852	RB	denotes	not
T5404	17858-17859	-RRB-	denotes	)
T5405	17859-17860	.	denotes	.
T5406	17860-17996	sentence	denotes	In contrast, PriA helicase, used as a control, bound to A-fork[3′] more efficiently than to A-fork[5′], as reported previously (23,25).
T5407	17861-17863	IN	denotes	In
T5408	17908-17913	VBD	denotes	bound
T5409	17864-17872	NN	denotes	contrast
T5410	17872-17874	,	denotes	,
T5411	17874-17878	NN	denotes	PriA
T5412	17879-17887	NN	denotes	helicase
T5413	17887-17889	,	denotes	,
T5414	17889-17893	VBN	denotes	used
T5415	17894-17896	IN	denotes	as
T5416	17897-17898	DT	denotes	a
T5417	17899-17906	NN	denotes	control
T5418	17906-17908	,	denotes	,
T5419	17914-17916	IN	denotes	to
T5420	17917-17918	NN	denotes	A
T5421	17919-17923	NN	denotes	fork
T5422	17918-17919	HYPH	denotes	-
T5423	17923-17924	-LRB-	denotes	[
T5424	17924-17925	CD	denotes	3
T5425	17925-17926	SYM	denotes	′
T5426	17926-17927	-RRB-	denotes	]
T5427	17928-17932	RBR	denotes	more
T5428	17933-17944	RB	denotes	efficiently
T5429	17945-17949	IN	denotes	than
T5430	17950-17952	IN	denotes	to
T5431	17953-17954	NN	denotes	A
T5432	17955-17959	NN	denotes	fork
T5433	17954-17955	HYPH	denotes	-
T5434	17959-17960	-LRB-	denotes	[
T5435	17960-17961	CD	denotes	5
T5436	17961-17962	SYM	denotes	′
T5437	17962-17963	-RRB-	denotes	]
T5438	17963-17965	,	denotes	,
T5439	17965-17967	IN	denotes	as
T5440	17968-17976	VBN	denotes	reported
T5441	17977-17987	RB	denotes	previously
T5442	17988-17989	-LRB-	denotes	(
T5443	17992-17994	CD	denotes	25
T5444	17989-17991	CD	denotes	23
T5445	17991-17992	,	denotes	,
T5446	17994-17995	-RRB-	denotes	)
T5447	17995-17996	.	denotes	.
T5448	17996-18139	sentence	denotes	Mcm4/6/7 bound also to 5′-extension and 3′-extension, albeit with slightly reduced affinity compared to A-fork[3′] and A-fork[5′] (Figure 4A).
T5449	17997-18001	NN	denotes	Mcm4
T5450	18006-18011	VBD	denotes	bound
T5451	18001-18002	HYPH	denotes	/
T5452	18002-18003	CD	denotes	6
T5453	18003-18004	HYPH	denotes	/
T5454	18004-18005	CD	denotes	7
T5455	18012-18016	RB	denotes	also
T5456	18017-18019	IN	denotes	to
T5457	18020-18021	CD	denotes	5
T5458	18023-18032	NN	denotes	extension
T5459	18021-18022	SYM	denotes	′
T5460	18022-18023	HYPH	denotes	-
T5461	18033-18036	CC	denotes	and
T5462	18037-18038	CD	denotes	3
T5463	18040-18049	NN	denotes	extension
T5464	18038-18039	SYM	denotes	′
T5465	18039-18040	HYPH	denotes	-
T5466	18049-18051	,	denotes	,
T5467	18051-18057	IN	denotes	albeit
T5468	18058-18062	IN	denotes	with
T5469	18063-18071	RB	denotes	slightly
T5470	18072-18079	VBN	denotes	reduced
T5471	18080-18088	NN	denotes	affinity
T5472	18089-18097	VBN	denotes	compared
T5473	18098-18100	IN	denotes	to
T5474	18101-18102	NN	denotes	A
T5475	18103-18107	NN	denotes	fork
T5476	18102-18103	HYPH	denotes	-
T5477	18107-18108	-LRB-	denotes	[
T5478	18108-18109	CD	denotes	3
T5479	18109-18110	SYM	denotes	′
T5480	18110-18111	-RRB-	denotes	]
T5481	18112-18115	CC	denotes	and
T5482	18116-18117	NN	denotes	A
T5483	18118-18122	NN	denotes	fork
T5484	18117-18118	HYPH	denotes	-
T5485	18122-18123	-LRB-	denotes	[
T5486	18123-18124	CD	denotes	5
T5487	18124-18125	SYM	denotes	′
T5488	18125-18126	-RRB-	denotes	]
T5489	18127-18128	-LRB-	denotes	(
T5490	18135-18137	NN	denotes	4A
T5491	18128-18134	NN	denotes	Figure
T5492	18137-18138	-RRB-	denotes	)
T5493	18138-18139	.	denotes	.
T5494	18139-18241	sentence	denotes	Mcm4/6/7 bound to A-fork[3′,5′] with much lower affinity, and the complex gave rise to a single band.
T5495	18140-18144	NN	denotes	Mcm4
T5496	18149-18154	VBD	denotes	bound
T5497	18144-18145	HYPH	denotes	/
T5498	18145-18146	CD	denotes	6
T5499	18146-18147	HYPH	denotes	/
T5500	18147-18148	CD	denotes	7
T5501	18155-18157	IN	denotes	to
T5502	18158-18159	NN	denotes	A
T5503	18160-18164	NN	denotes	fork
T5504	18159-18160	HYPH	denotes	-
T5505	18164-18165	-LRB-	denotes	[
T5506	18165-18166	CD	denotes	3
T5507	18166-18167	SYM	denotes	′
T5508	18167-18168	,	denotes	,
T5509	18168-18169	CD	denotes	5
T5510	18169-18170	SYM	denotes	′
T5511	18170-18171	-RRB-	denotes	]
T5512	18172-18176	IN	denotes	with
T5513	18177-18181	RB	denotes	much
T5514	18182-18187	JJR	denotes	lower
T5515	18188-18196	NN	denotes	affinity
T5516	18196-18198	,	denotes	,
T5517	18198-18201	CC	denotes	and
T5518	18202-18205	DT	denotes	the
T5519	18206-18213	NN	denotes	complex
T5520	18214-18218	VBD	denotes	gave
T5521	18219-18223	NN	denotes	rise
T5522	18224-18226	IN	denotes	to
T5523	18227-18228	DT	denotes	a
T5524	18236-18240	NN	denotes	band
T5525	18229-18235	JJ	denotes	single
T5526	18240-18241	.	denotes	.
T5527	18241-18423	sentence	denotes	These findings indicate that the Mcm4/6/7 complex is loaded onto DNA preferentially and efficiently through single-stranded region, regardless of the presence of 3′- or 5′-terminus.
T5528	18242-18247	DT	denotes	These
T5529	18248-18256	NNS	denotes	findings
T5530	18257-18265	VBP	denotes	indicate
T5531	18266-18270	IN	denotes	that
T5532	18295-18301	VBN	denotes	loaded
T5533	18271-18274	DT	denotes	the
T5534	18284-18291	NN	denotes	complex
T5535	18275-18279	NN	denotes	Mcm4
T5536	18279-18280	HYPH	denotes	/
T5537	18280-18281	CD	denotes	6
T5538	18281-18282	HYPH	denotes	/
T5539	18282-18283	CD	denotes	7
T5540	18292-18294	VBZ	denotes	is
T5541	18302-18306	IN	denotes	onto
T5542	18307-18310	NN	denotes	DNA
T5543	18311-18325	RB	denotes	preferentially
T5544	18326-18329	CC	denotes	and
T5545	18330-18341	RB	denotes	efficiently
T5546	18342-18349	IN	denotes	through
T5547	18350-18356	JJ	denotes	single
T5548	18357-18365	VBN	denotes	stranded
T5549	18356-18357	HYPH	denotes	-
T5550	18366-18372	NN	denotes	region
T5551	18372-18374	,	denotes	,
T5552	18374-18384	RB	denotes	regardless
T5553	18385-18387	IN	denotes	of
T5554	18388-18391	DT	denotes	the
T5555	18392-18400	NN	denotes	presence
T5556	18401-18403	IN	denotes	of
T5557	18404-18405	CD	denotes	3
T5558	18414-18422	NN	denotes	terminus
T5559	18405-18406	SYM	denotes	′
T5560	18406-18407	HYPH	denotes	-
T5561	18408-18410	CC	denotes	or
T5562	18411-18412	CD	denotes	5
T5563	18412-18413	SYM	denotes	′
T5564	18413-18414	HYPH	denotes	-
T5565	18422-18423	.	denotes	.
T5566	18423-18607	sentence	denotes	In contrast, only the substrate containing a 3′ single-stranded DNA tail (A-fork[5′]) was efficiently unwound and A-fork[3′] or A-fork[3′,5′], was not unwound by Mcm4/6/7 (Figure 4B).
T5567	18424-18426	IN	denotes	In
T5568	18526-18533	VBN	denotes	unwound
T5569	18427-18435	NN	denotes	contrast
T5570	18435-18437	,	denotes	,
T5571	18437-18441	RB	denotes	only
T5572	18446-18455	NN	denotes	substrate
T5573	18442-18445	DT	denotes	the
T5574	18456-18466	VBG	denotes	containing
T5575	18467-18468	DT	denotes	a
T5576	18492-18496	NN	denotes	tail
T5577	18469-18470	CD	denotes	3
T5578	18470-18471	SYM	denotes	′
T5579	18472-18478	JJ	denotes	single
T5580	18479-18487	VBN	denotes	stranded
T5581	18478-18479	HYPH	denotes	-
T5582	18488-18491	NN	denotes	DNA
T5583	18497-18498	-LRB-	denotes	(
T5584	18500-18504	NN	denotes	fork
T5585	18498-18499	NN	denotes	A
T5586	18499-18500	HYPH	denotes	-
T5587	18504-18505	-LRB-	denotes	[
T5588	18505-18506	CD	denotes	5
T5589	18506-18507	SYM	denotes	′
T5590	18507-18508	-RRB-	denotes	]
T5591	18508-18509	-RRB-	denotes	)
T5592	18510-18513	VBD	denotes	was
T5593	18514-18525	RB	denotes	efficiently
T5594	18534-18537	CC	denotes	and
T5595	18538-18539	NN	denotes	A
T5596	18540-18544	NN	denotes	fork
T5597	18539-18540	HYPH	denotes	-
T5598	18575-18582	VBN	denotes	unwound
T5599	18544-18545	-LRB-	denotes	[
T5600	18545-18546	CD	denotes	3
T5601	18546-18547	SYM	denotes	′
T5602	18547-18548	-RRB-	denotes	]
T5603	18549-18551	CC	denotes	or
T5604	18552-18553	NN	denotes	A
T5605	18554-18558	NN	denotes	fork
T5606	18553-18554	HYPH	denotes	-
T5607	18558-18559	-LRB-	denotes	[
T5608	18559-18560	CD	denotes	3
T5609	18560-18561	SYM	denotes	′
T5610	18561-18562	,	denotes	,
T5611	18562-18563	CD	denotes	5
T5612	18563-18564	SYM	denotes	′
T5613	18564-18565	-RRB-	denotes	]
T5614	18565-18567	,	denotes	,
T5615	18567-18570	VBD	denotes	was
T5616	18571-18574	RB	denotes	not
T5617	18583-18585	IN	denotes	by
T5618	18586-18590	NN	denotes	Mcm4
T5619	18590-18591	HYPH	denotes	/
T5620	18591-18592	CD	denotes	6
T5621	18592-18593	HYPH	denotes	/
T5622	18593-18594	CD	denotes	7
T5623	18595-18596	-LRB-	denotes	(
T5624	18603-18605	NN	denotes	4B
T5625	18596-18602	NN	denotes	Figure
T5626	18605-18606	-RRB-	denotes	)
T5627	18606-18607	.	denotes	.
T5628	18607-18784	sentence	denotes	Absence of helicase actions on A-fork[3′,5′] composed only of duplex regions is expected since double-stranded DNA is not able to activate the ATPase activity of Mcm4/6/7 (10).
T5629	18608-18615	NN	denotes	Absence
T5630	18688-18696	VBN	denotes	expected
T5631	18616-18618	IN	denotes	of
T5632	18619-18627	NN	denotes	helicase
T5633	18628-18635	NNS	denotes	actions
T5634	18636-18638	IN	denotes	on
T5635	18639-18640	NN	denotes	A
T5636	18641-18645	NN	denotes	fork
T5637	18640-18641	HYPH	denotes	-
T5638	18645-18646	-LRB-	denotes	[
T5639	18646-18647	CD	denotes	3
T5640	18647-18648	SYM	denotes	′
T5641	18648-18649	,	denotes	,
T5642	18649-18650	CD	denotes	5
T5643	18650-18651	SYM	denotes	′
T5644	18651-18652	-RRB-	denotes	]
T5645	18653-18661	VBN	denotes	composed
T5646	18662-18666	RB	denotes	only
T5647	18667-18669	IN	denotes	of
T5648	18670-18676	NN	denotes	duplex
T5649	18677-18684	NNS	denotes	regions
T5650	18685-18687	VBZ	denotes	is
T5651	18697-18702	IN	denotes	since
T5652	18723-18725	VBZ	denotes	is
T5653	18703-18709	JJ	denotes	double
T5654	18710-18718	VBN	denotes	stranded
T5655	18709-18710	HYPH	denotes	-
T5656	18719-18722	NN	denotes	DNA
T5657	18726-18729	RB	denotes	not
T5658	18730-18734	JJ	denotes	able
T5659	18735-18737	TO	denotes	to
T5660	18738-18746	VB	denotes	activate
T5661	18747-18750	DT	denotes	the
T5662	18758-18766	NN	denotes	activity
T5663	18751-18757	NN	denotes	ATPase
T5664	18767-18769	IN	denotes	of
T5665	18770-18774	NN	denotes	Mcm4
T5666	18774-18775	HYPH	denotes	/
T5667	18775-18776	CD	denotes	6
T5668	18776-18777	HYPH	denotes	/
T5669	18777-18778	CD	denotes	7
T5670	18779-18780	-LRB-	denotes	(
T5671	18780-18782	CD	denotes	10
T5672	18782-18783	-RRB-	denotes	)
T5673	18783-18784	.	denotes	.
T5674	18784-19035	sentence	denotes	In contrast, the PriA, a structure-specific helicase used as a control, binds at the fork junction and unwinds the fork and the nascent lagging strand DNA on A-fork[5′], or unwinds the nascent lagging strand on A-fork[3′,5′], as reported before (25).
T5675	18785-18787	IN	denotes	In
T5676	18857-18862	VBZ	denotes	binds
T5677	18788-18796	NN	denotes	contrast
T5678	18796-18798	,	denotes	,
T5679	18798-18801	DT	denotes	the
T5680	18802-18806	NN	denotes	PriA
T5681	18806-18808	,	denotes	,
T5682	18808-18809	DT	denotes	a
T5683	18829-18837	NN	denotes	helicase
T5684	18810-18819	NN	denotes	structure
T5685	18820-18828	JJ	denotes	specific
T5686	18819-18820	HYPH	denotes	-
T5687	18838-18842	VBN	denotes	used
T5688	18843-18845	IN	denotes	as
T5689	18846-18847	DT	denotes	a
T5690	18848-18855	NN	denotes	control
T5691	18855-18857	,	denotes	,
T5692	18863-18865	IN	denotes	at
T5693	18866-18869	DT	denotes	the
T5694	18875-18883	NN	denotes	junction
T5695	18870-18874	NN	denotes	fork
T5696	18884-18887	CC	denotes	and
T5697	18888-18895	VBZ	denotes	unwinds
T5698	18896-18899	DT	denotes	the
T5699	18900-18904	NN	denotes	fork
T5700	18905-18908	CC	denotes	and
T5701	18909-18912	DT	denotes	the
T5702	18936-18939	NN	denotes	DNA
T5703	18913-18920	JJ	denotes	nascent
T5704	18921-18928	VBG	denotes	lagging
T5705	18929-18935	NN	denotes	strand
T5706	18940-18942	IN	denotes	on
T5707	18943-18944	NN	denotes	A
T5708	18945-18949	NN	denotes	fork
T5709	18944-18945	HYPH	denotes	-
T5710	18949-18950	-LRB-	denotes	[
T5711	18950-18951	CD	denotes	5
T5712	18951-18952	SYM	denotes	′
T5713	18952-18953	-RRB-	denotes	]
T5714	18953-18955	,	denotes	,
T5715	18955-18957	CC	denotes	or
T5716	18958-18965	VBZ	denotes	unwinds
T5717	18966-18969	DT	denotes	the
T5718	18986-18992	NN	denotes	strand
T5719	18970-18977	JJ	denotes	nascent
T5720	18978-18985	VBG	denotes	lagging
T5721	18993-18995	IN	denotes	on
T5722	18996-18997	NN	denotes	A
T5723	18998-19002	NN	denotes	fork
T5724	18997-18998	HYPH	denotes	-
T5725	19002-19003	-LRB-	denotes	[
T5726	19003-19004	CD	denotes	3
T5727	19004-19005	SYM	denotes	′
T5728	19005-19006	,	denotes	,
T5729	19006-19007	CD	denotes	5
T5730	19007-19008	SYM	denotes	′
T5731	19008-19009	-RRB-	denotes	]
T5732	19009-19011	,	denotes	,
T5733	19011-19013	IN	denotes	as
T5734	19014-19022	VBN	denotes	reported
T5735	19023-19029	RB	denotes	before
T5736	19030-19031	-LRB-	denotes	(
T5737	19031-19033	CD	denotes	25
T5738	19033-19034	-RRB-	denotes	)
T5739	19034-19035	.	denotes	.
T5740	19035-19199	sentence	denotes	These results indicate that the Mcm4/6/7 complex binds to single-stranded DNA and that its helicase is activated only in the presence of a single-stranded 3′-tail.
T5741	19036-19041	DT	denotes	These
T5742	19042-19049	NNS	denotes	results
T5743	19050-19058	VBP	denotes	indicate
T5744	19059-19063	IN	denotes	that
T5745	19085-19090	VBZ	denotes	binds
T5746	19064-19067	DT	denotes	the
T5747	19077-19084	NN	denotes	complex
T5748	19068-19072	NN	denotes	Mcm4
T5749	19072-19073	HYPH	denotes	/
T5750	19073-19074	CD	denotes	6
T5751	19074-19075	HYPH	denotes	/
T5752	19075-19076	CD	denotes	7
T5753	19091-19093	IN	denotes	to
T5754	19094-19100	JJ	denotes	single
T5755	19101-19109	VBN	denotes	stranded
T5756	19100-19101	HYPH	denotes	-
T5757	19110-19113	NN	denotes	DNA
T5758	19114-19117	CC	denotes	and
T5759	19118-19122	IN	denotes	that
T5760	19139-19148	VBN	denotes	activated
T5761	19123-19126	PRP$	denotes	its
T5762	19127-19135	NN	denotes	helicase
T5763	19136-19138	VBZ	denotes	is
T5764	19149-19153	RB	denotes	only
T5765	19154-19156	IN	denotes	in
T5766	19157-19160	DT	denotes	the
T5767	19161-19169	NN	denotes	presence
T5768	19170-19172	IN	denotes	of
T5769	19173-19174	DT	denotes	a
T5770	19194-19198	NN	denotes	tail
T5771	19175-19181	JJ	denotes	single
T5772	19182-19190	VBN	denotes	stranded
T5773	19181-19182	HYPH	denotes	-
T5774	19191-19192	CD	denotes	3
T5775	19192-19193	SYM	denotes	′
T5776	19193-19194	HYPH	denotes	-
T5777	19198-19199	.	denotes	.
T5933	19201-19202	CD	denotes	3
T5934	19204-19213	NN	denotes	extension
T5935	19202-19203	SYM	denotes	′
T5936	19203-19204	HYPH	denotes	-
T5937	19236-19245	VBN	denotes	displaced
T5938	19214-19218	IN	denotes	with
T5939	19219-19223	NN	denotes	poly
T5940	19224-19226	NN	denotes	dT
T5941	19223-19224	-LRB-	denotes	(
T5942	19228-19232	NN	denotes	tail
T5943	19226-19227	-RRB-	denotes	)
T5944	19233-19235	VBZ	denotes	is
T5945	19246-19248	IN	denotes	by
T5946	19249-19253	NN	denotes	Mcm4
T5947	19253-19254	HYPH	denotes	/
T5948	19254-19255	CD	denotes	6
T5949	19255-19256	HYPH	denotes	/
T5950	19256-19257	CD	denotes	7
T5951	19257-19410	sentence	denotes	It has been reported that the 3′-extension can be unwound by the archaeal Mcm homohexamer but not by fission and budding yeast Mcm complexes (14,26,27).
T5952	19258-19260	PRP	denotes	It
T5953	19270-19278	VBN	denotes	reported
T5954	19261-19264	VBZ	denotes	has
T5955	19265-19269	VBN	denotes	been
T5956	19279-19283	IN	denotes	that
T5957	19308-19315	JJ	denotes	unwound
T5958	19284-19287	DT	denotes	the
T5959	19291-19300	NN	denotes	extension
T5960	19288-19289	CD	denotes	3
T5961	19289-19290	SYM	denotes	′
T5962	19290-19291	HYPH	denotes	-
T5963	19301-19304	MD	denotes	can
T5964	19305-19307	VB	denotes	be
T5965	19316-19318	IN	denotes	by
T5966	19319-19322	DT	denotes	the
T5967	19336-19347	NN	denotes	homohexamer
T5968	19323-19331	JJ	denotes	archaeal
T5969	19332-19335	NN	denotes	Mcm
T5970	19348-19351	CC	denotes	but
T5971	19352-19355	RB	denotes	not
T5972	19356-19358	IN	denotes	by
T5973	19359-19366	NN	denotes	fission
T5974	19389-19398	NNS	denotes	complexes
T5975	19367-19370	CC	denotes	and
T5976	19371-19378	NN	denotes	budding
T5977	19379-19384	NN	denotes	yeast
T5978	19385-19388	NN	denotes	Mcm
T5979	19399-19400	-LRB-	denotes	(
T5980	19406-19408	CD	denotes	27
T5981	19400-19402	CD	denotes	14
T5982	19402-19403	,	denotes	,
T5983	19403-19405	CD	denotes	26
T5984	19405-19406	,	denotes	,
T5985	19408-19409	-RRB-	denotes	)
T5986	19409-19410	.	denotes	.
T5987	19410-19590	sentence	denotes	We have constructed 3′-extension substrates containing poly(dT), poly(dA), poly(dC) and poly(dG) 3′-tails in order to examine whether mouse Mcm4/6/7 can displace these substrates.
T5988	19411-19413	PRP	denotes	We
T5989	19419-19430	VBN	denotes	constructed
T5990	19414-19418	VBP	denotes	have
T5991	19431-19432	CD	denotes	3
T5992	19434-19443	NN	denotes	extension
T5993	19432-19433	SYM	denotes	′
T5994	19433-19434	HYPH	denotes	-
T5995	19444-19454	NNS	denotes	substrates
T5996	19455-19465	VBG	denotes	containing
T5997	19466-19470	NN	denotes	poly
T5998	19471-19473	NN	denotes	dT
T5999	19470-19471	-LRB-	denotes	(
T6000	19511-19516	NNS	denotes	tails
T6001	19473-19474	-RRB-	denotes	)
T6002	19474-19476	,	denotes	,
T6003	19476-19480	NN	denotes	poly
T6004	19481-19483	NN	denotes	dA
T6005	19480-19481	-LRB-	denotes	(
T6006	19483-19484	-RRB-	denotes	)
T6007	19484-19486	,	denotes	,
T6008	19486-19490	NN	denotes	poly
T6009	19491-19493	NN	denotes	dC
T6010	19490-19491	-LRB-	denotes	(
T6011	19493-19494	-RRB-	denotes	)
T6012	19495-19498	CC	denotes	and
T6013	19499-19503	NN	denotes	poly
T6014	19504-19506	NN	denotes	dG
T6015	19503-19504	-LRB-	denotes	(
T6016	19506-19507	-RRB-	denotes	)
T6017	19508-19509	CD	denotes	3
T6018	19509-19510	SYM	denotes	′
T6019	19510-19511	HYPH	denotes	-
T6020	19517-19519	IN	denotes	in
T6021	19520-19525	NN	denotes	order
T6022	19526-19528	TO	denotes	to
T6023	19529-19536	VB	denotes	examine
T6024	19537-19544	IN	denotes	whether
T6025	19564-19572	VB	denotes	displace
T6026	19545-19550	NN	denotes	mouse
T6027	19551-19555	NN	denotes	Mcm4
T6028	19555-19556	HYPH	denotes	/
T6029	19556-19557	CD	denotes	6
T6030	19557-19558	HYPH	denotes	/
T6031	19558-19559	CD	denotes	7
T6032	19560-19563	MD	denotes	can
T6033	19573-19578	DT	denotes	these
T6034	19579-19589	NNS	denotes	substrates
T6035	19589-19590	.	denotes	.
T6036	19590-19741	sentence	denotes	We first found that Mcm4/6/7 binds to dT-, dC- and dG-3′-extension with similar affinity, and to dA-3′-extension with much lower affinity (Figure 5A).
T6037	19591-19593	PRP	denotes	We
T6038	19600-19605	VBD	denotes	found
T6039	19594-19599	RB	denotes	first
T6040	19606-19610	IN	denotes	that
T6041	19620-19625	VBZ	denotes	binds
T6042	19611-19615	NN	denotes	Mcm4
T6043	19615-19616	HYPH	denotes	/
T6044	19616-19617	CD	denotes	6
T6045	19617-19618	HYPH	denotes	/
T6046	19618-19619	CD	denotes	7
T6047	19626-19628	IN	denotes	to
T6048	19629-19631	NN	denotes	dT
T6049	19648-19657	NN	denotes	extension
T6050	19631-19632	HYPH	denotes	-
T6051	19632-19634	,	denotes	,
T6052	19634-19636	NN	denotes	dC
T6053	19636-19637	HYPH	denotes	-
T6054	19638-19641	CC	denotes	and
T6055	19642-19644	NN	denotes	dG
T6056	19644-19645	HYPH	denotes	-
T6057	19645-19646	CD	denotes	3
T6058	19646-19647	SYM	denotes	′
T6059	19647-19648	HYPH	denotes	-
T6060	19658-19662	IN	denotes	with
T6061	19663-19670	JJ	denotes	similar
T6062	19671-19679	NN	denotes	affinity
T6063	19679-19681	,	denotes	,
T6064	19681-19684	CC	denotes	and
T6065	19685-19687	IN	denotes	to
T6066	19688-19690	NN	denotes	dA
T6067	19694-19703	NN	denotes	extension
T6068	19690-19691	HYPH	denotes	-
T6069	19691-19692	CD	denotes	3
T6070	19692-19693	SYM	denotes	′
T6071	19693-19694	HYPH	denotes	-
T6072	19704-19708	IN	denotes	with
T6073	19709-19713	RB	denotes	much
T6074	19714-19719	JJR	denotes	lower
T6075	19720-19728	NN	denotes	affinity
T6076	19729-19730	-LRB-	denotes	(
T6077	19737-19739	NN	denotes	5A
T6078	19730-19736	NN	denotes	Figure
T6079	19739-19740	-RRB-	denotes	)
T6080	19740-19741	.	denotes	.
T6081	19741-19872	sentence	denotes	This is consistent with the previous results that Mcm4/6/7 binds to 50mer oligo-dT, -dC and -dG, but not to a 50mer oligo-dA (13).
T6082	19742-19746	DT	denotes	This
T6083	19747-19749	VBZ	denotes	is
T6084	19750-19760	JJ	denotes	consistent
T6085	19761-19765	IN	denotes	with
T6086	19766-19769	DT	denotes	the
T6087	19779-19786	NNS	denotes	results
T6088	19770-19778	JJ	denotes	previous
T6089	19787-19791	IN	denotes	that
T6090	19801-19806	VBZ	denotes	binds
T6091	19792-19796	NN	denotes	Mcm4
T6092	19796-19797	HYPH	denotes	/
T6093	19797-19798	CD	denotes	6
T6094	19798-19799	HYPH	denotes	/
T6095	19799-19800	CD	denotes	7
T6096	19807-19809	IN	denotes	to
T6097	19810-19815	NN	denotes	50mer
T6098	19822-19824	NN	denotes	dT
T6099	19816-19821	NN	denotes	oligo
T6100	19821-19822	HYPH	denotes	-
T6101	19824-19826	,	denotes	,
T6102	19826-19827	HYPH	denotes	-
T6103	19827-19829	NN	denotes	dC
T6104	19830-19833	CC	denotes	and
T6105	19834-19835	HYPH	denotes	-
T6106	19835-19837	NN	denotes	dG
T6107	19837-19839	,	denotes	,
T6108	19839-19842	CC	denotes	but
T6109	19843-19846	RB	denotes	not
T6110	19847-19849	IN	denotes	to
T6111	19850-19851	DT	denotes	a
T6112	19864-19866	NN	denotes	dA
T6113	19852-19857	NN	denotes	50mer
T6114	19858-19863	NN	denotes	oligo
T6115	19863-19864	HYPH	denotes	-
T6116	19867-19868	-LRB-	denotes	(
T6117	19868-19870	CD	denotes	13
T6118	19870-19871	-RRB-	denotes	)
T6119	19871-19872	.	denotes	.
T6120	19872-20040	sentence	denotes	We found that dT-tailed 3′-extension was displaced efficiently by Mcm 4/6/7 while almost no or very little activity was detected on other substrates (Figure 5B and C).
T6121	19873-19875	PRP	denotes	We
T6122	19876-19881	VBD	denotes	found
T6123	19882-19886	IN	denotes	that
T6124	19914-19923	VBN	denotes	displaced
T6125	19887-19889	NN	denotes	dT
T6126	19890-19896	VBN	denotes	tailed
T6127	19889-19890	HYPH	denotes	-
T6128	19900-19909	NN	denotes	extension
T6129	19897-19898	CD	denotes	3
T6130	19898-19899	SYM	denotes	′
T6131	19899-19900	HYPH	denotes	-
T6132	19910-19913	VBD	denotes	was
T6133	19924-19935	RB	denotes	efficiently
T6134	19936-19938	IN	denotes	by
T6135	19939-19942	NN	denotes	Mcm
T6136	19943-19944	CD	denotes	4
T6137	19944-19945	HYPH	denotes	/
T6138	19945-19946	CD	denotes	6
T6139	19946-19947	HYPH	denotes	/
T6140	19947-19948	CD	denotes	7
T6141	19949-19954	IN	denotes	while
T6142	19993-20001	VBN	denotes	detected
T6143	19955-19961	RB	denotes	almost
T6144	19962-19964	DT	denotes	no
T6145	19965-19967	CC	denotes	or
T6146	19968-19972	RB	denotes	very
T6147	19973-19979	JJ	denotes	little
T6148	19980-19988	NN	denotes	activity
T6149	19989-19992	VBD	denotes	was
T6150	20002-20004	IN	denotes	on
T6151	20005-20010	JJ	denotes	other
T6152	20011-20021	NNS	denotes	substrates
T6153	20022-20023	-LRB-	denotes	(
T6154	20030-20032	NN	denotes	5B
T6155	20023-20029	NN	denotes	Figure
T6156	20033-20036	CC	denotes	and
T6157	20037-20038	NN	denotes	C
T6158	20038-20039	-RRB-	denotes	)
T6159	20039-20040	.	denotes	.
T6160	20040-20154	sentence	denotes	This is in sharp contrast to yeast Mcm4/6/7 helicases which are not capable of unwinding 3′-extension substrates.
T6161	20041-20045	DT	denotes	This
T6162	20046-20048	VBZ	denotes	is
T6163	20049-20051	IN	denotes	in
T6164	20052-20057	JJ	denotes	sharp
T6165	20058-20066	NN	denotes	contrast
T6166	20067-20069	IN	denotes	to
T6167	20070-20075	NN	denotes	yeast
T6168	20085-20094	NNS	denotes	helicases
T6169	20076-20080	NN	denotes	Mcm4
T6170	20080-20081	HYPH	denotes	/
T6171	20081-20082	CD	denotes	6
T6172	20082-20083	HYPH	denotes	/
T6173	20083-20084	CD	denotes	7
T6174	20095-20100	WDT	denotes	which
T6175	20101-20104	VBP	denotes	are
T6176	20105-20108	RB	denotes	not
T6177	20109-20116	JJ	denotes	capable
T6178	20117-20119	IN	denotes	of
T6179	20120-20129	VBG	denotes	unwinding
T6180	20130-20131	CD	denotes	3
T6181	20133-20142	NN	denotes	extension
T6182	20131-20132	SYM	denotes	′
T6183	20132-20133	HYPH	denotes	-
T6184	20143-20153	NNS	denotes	substrates
T6185	20153-20154	.	denotes	.
T6186	20154-20260	sentence	denotes	Furthermore, dT-tailed 5′-extension, to which Mcm4/6/7 could bind, was not displaced by the Mcm helicase.
T6187	20155-20166	RB	denotes	Furthermore
T6188	20230-20239	VBN	denotes	displaced
T6189	20166-20168	,	denotes	,
T6190	20168-20170	NN	denotes	dT
T6191	20171-20177	VBN	denotes	tailed
T6192	20170-20171	HYPH	denotes	-
T6193	20181-20190	NN	denotes	extension
T6194	20178-20179	CD	denotes	5
T6195	20179-20180	SYM	denotes	′
T6196	20180-20181	HYPH	denotes	-
T6197	20190-20192	,	denotes	,
T6198	20192-20194	IN	denotes	to
T6199	20216-20220	VB	denotes	bind
T6200	20195-20200	WDT	denotes	which
T6201	20201-20205	NN	denotes	Mcm4
T6202	20205-20206	HYPH	denotes	/
T6203	20206-20207	CD	denotes	6
T6204	20207-20208	HYPH	denotes	/
T6205	20208-20209	CD	denotes	7
T6206	20210-20215	MD	denotes	could
T6207	20220-20222	,	denotes	,
T6208	20222-20225	VBD	denotes	was
T6209	20226-20229	RB	denotes	not
T6210	20240-20242	IN	denotes	by
T6211	20243-20246	DT	denotes	the
T6212	20251-20259	NN	denotes	helicase
T6213	20247-20250	NN	denotes	Mcm
T6214	20259-20260	.	denotes	.
T6215	20260-20451	sentence	denotes	The addition of the 5′-tail to dT-tailed 3′-extension further stimulated the displacement (Figure 5D), confirming the previous report that the fork structures facilitate the helicase action.
T6216	20261-20264	DT	denotes	The
T6217	20265-20273	NN	denotes	addition
T6218	20323-20333	VBD	denotes	stimulated
T6219	20274-20276	IN	denotes	of
T6220	20277-20280	DT	denotes	the
T6221	20284-20288	NN	denotes	tail
T6222	20281-20282	CD	denotes	5
T6223	20282-20283	SYM	denotes	′
T6224	20283-20284	HYPH	denotes	-
T6225	20289-20291	IN	denotes	to
T6226	20292-20294	NN	denotes	dT
T6227	20295-20301	VBN	denotes	tailed
T6228	20294-20295	HYPH	denotes	-
T6229	20305-20314	NN	denotes	extension
T6230	20302-20303	CD	denotes	3
T6231	20303-20304	SYM	denotes	′
T6232	20304-20305	HYPH	denotes	-
T6233	20315-20322	RB	denotes	further
T6234	20334-20337	DT	denotes	the
T6235	20338-20350	NN	denotes	displacement
T6236	20351-20352	-LRB-	denotes	(
T6237	20359-20361	NN	denotes	5D
T6238	20352-20358	NN	denotes	Figure
T6239	20361-20362	-RRB-	denotes	)
T6240	20362-20364	,	denotes	,
T6241	20364-20374	VBG	denotes	confirming
T6242	20375-20378	DT	denotes	the
T6243	20388-20394	NN	denotes	report
T6244	20379-20387	JJ	denotes	previous
T6245	20395-20399	IN	denotes	that
T6246	20420-20430	VBP	denotes	facilitate
T6247	20400-20403	DT	denotes	the
T6248	20409-20419	NNS	denotes	structures
T6249	20404-20408	NN	denotes	fork
T6250	20431-20434	DT	denotes	the
T6251	20444-20450	NN	denotes	action
T6252	20435-20443	NN	denotes	helicase
T6253	20450-20451	.	denotes	.
T6254	20451-20615	sentence	denotes	Thus, these results further strengthen our conclusion that the mammalian Mcm helicase is generally activated by the thymine-stretch on the 3′-single-stranded tail.
T6255	20452-20456	RB	denotes	Thus
T6256	20480-20490	VB	denotes	strengthen
T6257	20456-20458	,	denotes	,
T6258	20458-20463	DT	denotes	these
T6259	20464-20471	NNS	denotes	results
T6260	20472-20479	RB	denotes	further
T6261	20491-20494	PRP$	denotes	our
T6262	20495-20505	NN	denotes	conclusion
T6263	20506-20510	IN	denotes	that
T6264	20551-20560	VBN	denotes	activated
T6265	20511-20514	DT	denotes	the
T6266	20529-20537	NN	denotes	helicase
T6267	20515-20524	JJ	denotes	mammalian
T6268	20525-20528	NN	denotes	Mcm
T6269	20538-20540	VBZ	denotes	is
T6270	20541-20550	RB	denotes	generally
T6271	20561-20563	IN	denotes	by
T6272	20564-20567	DT	denotes	the
T6273	20576-20583	NN	denotes	stretch
T6274	20568-20575	NN	denotes	thymine
T6275	20575-20576	HYPH	denotes	-
T6276	20584-20586	IN	denotes	on
T6277	20587-20590	DT	denotes	the
T6278	20610-20614	NN	denotes	tail
T6279	20591-20592	CD	denotes	3
T6280	20592-20593	SYM	denotes	′
T6281	20593-20594	HYPH	denotes	-
T6282	20594-20600	JJ	denotes	single
T6283	20601-20609	VBN	denotes	stranded
T6284	20600-20601	HYPH	denotes	-
T6285	20614-20615	.	denotes	.
T6404	20617-20620	DT	denotes	The
T6405	20621-20628	NNS	denotes	effects
T6406	20629-20631	IN	denotes	of
T6407	20632-20635	DT	denotes	the
T6408	20644-20651	NN	denotes	content
T6409	20636-20643	NN	denotes	thymine
T6410	20652-20654	IN	denotes	of
T6411	20655-20658	DT	denotes	the
T6412	20662-20666	NN	denotes	tail
T6413	20659-20660	CD	denotes	3
T6414	20660-20661	SYM	denotes	′
T6415	20661-20662	HYPH	denotes	-
T6416	20667-20669	IN	denotes	on
T6417	20670-20673	NN	denotes	Mcm
T6418	20674-20682	NN	denotes	helicase
T6419	20683-20691	NN	denotes	activity
T6420	20691-20828	sentence	denotes	We next examined more systematically the effect of the compositions of the thymine residues of the 3′-tail on the Mcm helicase activity.
T6421	20692-20694	PRP	denotes	We
T6422	20700-20708	VBD	denotes	examined
T6423	20695-20699	RB	denotes	next
T6424	20709-20713	RBR	denotes	more
T6425	20714-20728	RB	denotes	systematically
T6426	20729-20732	DT	denotes	the
T6427	20733-20739	NN	denotes	effect
T6428	20740-20742	IN	denotes	of
T6429	20743-20746	DT	denotes	the
T6430	20747-20759	NNS	denotes	compositions
T6431	20760-20762	IN	denotes	of
T6432	20763-20766	DT	denotes	the
T6433	20775-20783	NNS	denotes	residues
T6434	20767-20774	NN	denotes	thymine
T6435	20784-20786	IN	denotes	of
T6436	20787-20790	DT	denotes	the
T6437	20794-20798	NN	denotes	tail
T6438	20791-20792	CD	denotes	3
T6439	20792-20793	SYM	denotes	′
T6440	20793-20794	HYPH	denotes	-
T6441	20799-20801	IN	denotes	on
T6442	20802-20805	DT	denotes	the
T6443	20819-20827	NN	denotes	activity
T6444	20806-20809	NN	denotes	Mcm
T6445	20810-20818	NN	denotes	helicase
T6446	20827-20828	.	denotes	.
T6447	20828-21075	sentence	denotes	A partial heteroduplex M13 single-stranded DNA containing a 37 bp duplex region in the presence or absence of the 50 nt long 3′-tail of various nucleotide compositions were constructed and the unwinding by mouse Mcm4/6/7 was examined (Figure 6A).
T6448	20829-20830	DT	denotes	A
T6449	20872-20875	NN	denotes	DNA
T6450	20831-20838	JJ	denotes	partial
T6451	20839-20851	NN	denotes	heteroduplex
T6452	20852-20855	NN	denotes	M13
T6453	20856-20862	JJ	denotes	single
T6454	20863-20871	VBN	denotes	stranded
T6455	20862-20863	HYPH	denotes	-
T6456	21002-21013	VBN	denotes	constructed
T6457	20876-20886	VBG	denotes	containing
T6458	20887-20888	DT	denotes	a
T6459	20902-20908	NN	denotes	region
T6460	20889-20891	CD	denotes	37
T6461	20892-20894	NN	denotes	bp
T6462	20895-20901	NN	denotes	duplex
T6463	20909-20911	IN	denotes	in
T6464	20912-20915	DT	denotes	the
T6465	20916-20924	NN	denotes	presence
T6466	20925-20927	CC	denotes	or
T6467	20928-20935	NN	denotes	absence
T6468	20936-20938	IN	denotes	of
T6469	20939-20942	DT	denotes	the
T6470	20957-20961	NN	denotes	tail
T6471	20943-20945	CD	denotes	50
T6472	20946-20948	NN	denotes	nt
T6473	20949-20953	JJ	denotes	long
T6474	20954-20955	CD	denotes	3
T6475	20955-20956	SYM	denotes	′
T6476	20956-20957	HYPH	denotes	-
T6477	20962-20964	IN	denotes	of
T6478	20965-20972	JJ	denotes	various
T6479	20984-20996	NNS	denotes	compositions
T6480	20973-20983	NN	denotes	nucleotide
T6481	20997-21001	VBD	denotes	were
T6482	21014-21017	CC	denotes	and
T6483	21018-21021	DT	denotes	the
T6484	21022-21031	NN	denotes	unwinding
T6485	21054-21062	VBN	denotes	examined
T6486	21032-21034	IN	denotes	by
T6487	21035-21040	NN	denotes	mouse
T6488	21041-21045	NN	denotes	Mcm4
T6489	21045-21046	HYPH	denotes	/
T6490	21046-21047	CD	denotes	6
T6491	21047-21048	HYPH	denotes	/
T6492	21048-21049	CD	denotes	7
T6493	21050-21053	VBD	denotes	was
T6494	21063-21064	-LRB-	denotes	(
T6495	21071-21073	NN	denotes	6A
T6496	21064-21070	NN	denotes	Figure
T6497	21073-21074	-RRB-	denotes	)
T6498	21074-21075	.	denotes	.
T6499	21075-21203	sentence	denotes	Due to its low processivity, Mcm4/6/7 barely displaced the annealed 37mer oligonucleotide without a 3′-tail (37mer; Figure 6A).
T6500	21076-21079	IN	denotes	Due
T6501	21121-21130	VBD	denotes	displaced
T6502	21080-21082	IN	denotes	to
T6503	21083-21086	PRP$	denotes	its
T6504	21091-21103	NN	denotes	processivity
T6505	21087-21090	JJ	denotes	low
T6506	21103-21105	,	denotes	,
T6507	21105-21109	NN	denotes	Mcm4
T6508	21109-21110	HYPH	denotes	/
T6509	21110-21111	CD	denotes	6
T6510	21111-21112	HYPH	denotes	/
T6511	21112-21113	CD	denotes	7
T6512	21114-21120	RB	denotes	barely
T6513	21131-21134	DT	denotes	the
T6514	21150-21165	NN	denotes	oligonucleotide
T6515	21135-21143	JJ	denotes	annealed
T6516	21144-21149	NN	denotes	37mer
T6517	21166-21173	IN	denotes	without
T6518	21174-21175	DT	denotes	a
T6519	21179-21183	NN	denotes	tail
T6520	21176-21177	CD	denotes	3
T6521	21177-21178	SYM	denotes	′
T6522	21178-21179	HYPH	denotes	-
T6523	21184-21185	-LRB-	denotes	(
T6524	21199-21201	NN	denotes	6A
T6525	21185-21190	NN	denotes	37mer
T6526	21190-21191	:	denotes	;
T6527	21192-21198	NN	denotes	Figure
T6528	21201-21202	-RRB-	denotes	)
T6529	21202-21203	.	denotes	.
T6530	21203-21396	sentence	denotes	Only weak helicase activity was observed on the substrate carrying the 3′-tail of 25 repeats of TA dinucleotide (37-TA25; Figure 6A), whereas strong helicase activity was observed on 37-TTA17.
T6531	21204-21208	RB	denotes	Only
T6532	21223-21231	NN	denotes	activity
T6533	21209-21213	JJ	denotes	weak
T6534	21214-21222	NN	denotes	helicase
T6535	21236-21244	VBN	denotes	observed
T6536	21232-21235	VBD	denotes	was
T6537	21245-21247	IN	denotes	on
T6538	21248-21251	DT	denotes	the
T6539	21252-21261	NN	denotes	substrate
T6540	21262-21270	VBG	denotes	carrying
T6541	21271-21274	DT	denotes	the
T6542	21278-21282	NN	denotes	tail
T6543	21275-21276	CD	denotes	3
T6544	21276-21277	SYM	denotes	′
T6545	21277-21278	HYPH	denotes	-
T6546	21283-21285	IN	denotes	of
T6547	21286-21288	CD	denotes	25
T6548	21289-21296	NNS	denotes	repeats
T6549	21297-21299	IN	denotes	of
T6550	21300-21302	NN	denotes	TA
T6551	21303-21315	NN	denotes	dinucleotide
T6552	21316-21317	-LRB-	denotes	(
T6553	21333-21335	NN	denotes	6A
T6554	21317-21319	CD	denotes	37
T6555	21320-21324	NN	denotes	TA25
T6556	21319-21320	HYPH	denotes	-
T6557	21324-21325	:	denotes	;
T6558	21326-21332	NN	denotes	Figure
T6559	21335-21336	-RRB-	denotes	)
T6560	21336-21338	,	denotes	,
T6561	21338-21345	IN	denotes	whereas
T6562	21375-21383	VBN	denotes	observed
T6563	21346-21352	JJ	denotes	strong
T6564	21362-21370	NN	denotes	activity
T6565	21353-21361	NN	denotes	helicase
T6566	21371-21374	VBD	denotes	was
T6567	21384-21386	IN	denotes	on
T6568	21387-21389	CD	denotes	37
T6569	21390-21395	NN	denotes	TTA17
T6570	21389-21390	HYPH	denotes	-
T6571	21395-21396	.	denotes	.
T6572	21396-21646	sentence	denotes	Increase of thymine content on the 3′-tail did not further increase the extent of unwinding (37-TTTA13, 37-TTTTA10 and 37-T50; Figure 6A), suggesting that 17 repeats of TT dinucleotides (67% thymine) may be sufficient for activation of Mcm helicase.
T6573	21397-21405	NN	denotes	Increase
T6574	21456-21464	VB	denotes	increase
T6575	21406-21408	IN	denotes	of
T6576	21409-21416	NN	denotes	thymine
T6577	21417-21424	NN	denotes	content
T6578	21425-21427	IN	denotes	on
T6579	21428-21431	DT	denotes	the
T6580	21435-21439	NN	denotes	tail
T6581	21432-21433	CD	denotes	3
T6582	21433-21434	SYM	denotes	′
T6583	21434-21435	HYPH	denotes	-
T6584	21440-21443	VBD	denotes	did
T6585	21444-21447	RB	denotes	not
T6586	21448-21455	RB	denotes	further
T6587	21465-21468	DT	denotes	the
T6588	21469-21475	NN	denotes	extent
T6589	21476-21478	IN	denotes	of
T6590	21479-21488	NN	denotes	unwinding
T6591	21489-21490	-LRB-	denotes	(
T6592	21531-21533	NN	denotes	6A
T6593	21490-21492	CD	denotes	37
T6594	21493-21499	NN	denotes	TTTA13
T6595	21492-21493	HYPH	denotes	-
T6596	21499-21501	,	denotes	,
T6597	21501-21503	CD	denotes	37
T6598	21504-21511	NN	denotes	TTTTA10
T6599	21503-21504	HYPH	denotes	-
T6600	21512-21515	CC	denotes	and
T6601	21516-21518	CD	denotes	37
T6602	21519-21522	NN	denotes	T50
T6603	21518-21519	HYPH	denotes	-
T6604	21522-21523	:	denotes	;
T6605	21524-21530	NN	denotes	Figure
T6606	21533-21534	-RRB-	denotes	)
T6607	21534-21536	,	denotes	,
T6608	21536-21546	VBG	denotes	suggesting
T6609	21547-21551	IN	denotes	that
T6610	21601-21603	VB	denotes	be
T6611	21552-21554	CD	denotes	17
T6612	21555-21562	NNS	denotes	repeats
T6613	21563-21565	IN	denotes	of
T6614	21566-21568	NN	denotes	TT
T6615	21569-21582	NNS	denotes	dinucleotides
T6616	21583-21584	-LRB-	denotes	(
T6617	21588-21595	NN	denotes	thymine
T6618	21584-21586	CD	denotes	67
T6619	21586-21587	NN	denotes	%
T6620	21595-21596	-RRB-	denotes	)
T6621	21597-21600	MD	denotes	may
T6622	21604-21614	JJ	denotes	sufficient
T6623	21615-21618	IN	denotes	for
T6624	21619-21629	NN	denotes	activation
T6625	21630-21632	IN	denotes	of
T6626	21633-21636	NN	denotes	Mcm
T6627	21637-21645	NN	denotes	helicase
T6628	21645-21646	.	denotes	.
T6629	21646-21847	sentence	denotes	It should be noted that the TA25 tail would self-anneal and this would prevent the loading of Mcm, since the 3′-tail containing TTAA13 or TTTAAA8 did not enhance the unwinding either (data not shown).
T6630	21647-21649	PRP	denotes	It
T6631	21660-21665	VBN	denotes	noted
T6632	21650-21656	MD	denotes	should
T6633	21657-21659	VB	denotes	be
T6634	21666-21670	IN	denotes	that
T6635	21696-21702	VB	denotes	anneal
T6636	21671-21674	DT	denotes	the
T6637	21680-21684	NN	denotes	tail
T6638	21675-21679	NN	denotes	TA25
T6639	21685-21690	MD	denotes	would
T6640	21691-21695	NN	denotes	self
T6641	21695-21696	HYPH	denotes	-
T6642	21703-21706	CC	denotes	and
T6643	21707-21711	DT	denotes	this
T6644	21718-21725	VB	denotes	prevent
T6645	21712-21717	MD	denotes	would
T6646	21726-21729	DT	denotes	the
T6647	21730-21737	NN	denotes	loading
T6648	21738-21740	IN	denotes	of
T6649	21741-21744	NN	denotes	Mcm
T6650	21744-21746	,	denotes	,
T6651	21746-21751	IN	denotes	since
T6652	21801-21808	VB	denotes	enhance
T6653	21752-21755	DT	denotes	the
T6654	21759-21763	NN	denotes	tail
T6655	21756-21757	CD	denotes	3
T6656	21757-21758	SYM	denotes	′
T6657	21758-21759	HYPH	denotes	-
T6658	21764-21774	VBG	denotes	containing
T6659	21775-21781	NN	denotes	TTAA13
T6660	21782-21784	CC	denotes	or
T6661	21785-21792	NN	denotes	TTTAAA8
T6662	21793-21796	VBD	denotes	did
T6663	21797-21800	RB	denotes	not
T6664	21809-21812	DT	denotes	the
T6665	21813-21822	NN	denotes	unwinding
T6666	21823-21829	RB	denotes	either
T6667	21830-21831	-LRB-	denotes	(
T6668	21840-21845	VBN	denotes	shown
T6669	21831-21835	NNS	denotes	data
T6670	21836-21839	RB	denotes	not
T6671	21845-21846	-RRB-	denotes	)
T6672	21846-21847	.	denotes	.
T6673	21847-21947	sentence	denotes	Therefore, we next examined the effects of repeating sequences of thymine and cytosine (Figure 6B).
T6674	21848-21857	RB	denotes	Therefore
T6675	21867-21875	VBD	denotes	examined
T6676	21857-21859	,	denotes	,
T6677	21859-21861	PRP	denotes	we
T6678	21862-21866	RB	denotes	next
T6679	21876-21879	DT	denotes	the
T6680	21880-21887	NNS	denotes	effects
T6681	21888-21890	IN	denotes	of
T6682	21891-21900	VBG	denotes	repeating
T6683	21901-21910	NNS	denotes	sequences
T6684	21911-21913	IN	denotes	of
T6685	21914-21921	NN	denotes	thymine
T6686	21922-21925	CC	denotes	and
T6687	21926-21934	NN	denotes	cytosine
T6688	21935-21936	-LRB-	denotes	(
T6689	21943-21945	NN	denotes	6B
T6690	21936-21942	NN	denotes	Figure
T6691	21945-21946	-RRB-	denotes	)
T6692	21946-21947	.	denotes	.
T6693	21947-22221	sentence	denotes	Partial heteroduplex substrates containing the 3′-tail of TC25, TTCC13, TTTCCC8, TTTTCCCC6, TTCC17 were displaced by the Mcm4/6/7 complex to the extent similar to that achieved by TTA17, suggesting that 50% thymine content is sufficient for full activation of Mcm helicase.
T6694	21948-21955	JJ	denotes	Partial
T6695	21969-21979	NNS	denotes	substrates
T6696	21956-21968	NN	denotes	heteroduplex
T6697	22052-22061	VBN	denotes	displaced
T6698	21980-21990	VBG	denotes	containing
T6699	21991-21994	DT	denotes	the
T6700	21998-22002	NN	denotes	tail
T6701	21995-21996	CD	denotes	3
T6702	21996-21997	SYM	denotes	′
T6703	21997-21998	HYPH	denotes	-
T6704	22003-22005	IN	denotes	of
T6705	22006-22010	NN	denotes	TC25
T6706	22040-22046	NN	denotes	TTCC17
T6707	22010-22012	,	denotes	,
T6708	22012-22018	NN	denotes	TTCC13
T6709	22018-22020	,	denotes	,
T6710	22020-22027	NN	denotes	TTTCCC8
T6711	22027-22029	,	denotes	,
T6712	22029-22038	NN	denotes	TTTTCCCC6
T6713	22038-22040	,	denotes	,
T6714	22047-22051	VBD	denotes	were
T6715	22062-22064	IN	denotes	by
T6716	22065-22068	DT	denotes	the
T6717	22078-22085	NN	denotes	complex
T6718	22069-22073	NN	denotes	Mcm4
T6719	22073-22074	HYPH	denotes	/
T6720	22074-22075	CD	denotes	6
T6721	22075-22076	HYPH	denotes	/
T6722	22076-22077	CD	denotes	7
T6723	22086-22088	IN	denotes	to
T6724	22089-22092	DT	denotes	the
T6725	22093-22099	NN	denotes	extent
T6726	22100-22107	JJ	denotes	similar
T6727	22108-22110	IN	denotes	to
T6728	22111-22115	DT	denotes	that
T6729	22116-22124	VBN	denotes	achieved
T6730	22125-22127	IN	denotes	by
T6731	22128-22133	NN	denotes	TTA17
T6732	22133-22135	,	denotes	,
T6733	22135-22145	VBG	denotes	suggesting
T6734	22146-22150	IN	denotes	that
T6735	22171-22173	VBZ	denotes	is
T6736	22151-22153	CD	denotes	50
T6737	22153-22154	NN	denotes	%
T6738	22163-22170	NN	denotes	content
T6739	22155-22162	NN	denotes	thymine
T6740	22174-22184	JJ	denotes	sufficient
T6741	22185-22188	IN	denotes	for
T6742	22189-22193	JJ	denotes	full
T6743	22194-22204	NN	denotes	activation
T6744	22205-22207	IN	denotes	of
T6745	22208-22211	NN	denotes	Mcm
T6746	22212-22220	NN	denotes	helicase
T6747	22220-22221	.	denotes	.
T6748	22221-22363	sentence	denotes	TTCC13 and TTCC17 were displaced to a similar extent, consistent with the earlier result that a 40 nt tail is sufficient for activation (13).
T6749	22222-22228	NN	denotes	TTCC13
T6750	22245-22254	VBN	denotes	displaced
T6751	22229-22232	CC	denotes	and
T6752	22233-22239	NN	denotes	TTCC17
T6753	22240-22244	VBD	denotes	were
T6754	22255-22257	IN	denotes	to
T6755	22258-22259	DT	denotes	a
T6756	22268-22274	NN	denotes	extent
T6757	22260-22267	JJ	denotes	similar
T6758	22274-22276	,	denotes	,
T6759	22276-22286	JJ	denotes	consistent
T6760	22287-22291	IN	denotes	with
T6761	22292-22295	DT	denotes	the
T6762	22304-22310	NN	denotes	result
T6763	22296-22303	JJR	denotes	earlier
T6764	22311-22315	IN	denotes	that
T6765	22329-22331	VBZ	denotes	is
T6766	22316-22317	DT	denotes	a
T6767	22324-22328	NN	denotes	tail
T6768	22318-22320	CD	denotes	40
T6769	22321-22323	NN	denotes	nt
T6770	22332-22342	JJ	denotes	sufficient
T6771	22343-22346	IN	denotes	for
T6772	22347-22357	NN	denotes	activation
T6773	22358-22359	-LRB-	denotes	(
T6774	22359-22361	CD	denotes	13
T6775	22361-22362	-RRB-	denotes	)
T6776	22362-22363	.	denotes	.
T6777	22363-22525	sentence	denotes	However, the efficiency of the displacement significantly decreased as the thymine content of the 3′-tail dropped to 33%, as in TAA17 or TCC17 (Figure 6B and C).
T6778	22364-22371	RB	denotes	However
T6779	22422-22431	VBD	denotes	decreased
T6780	22371-22373	,	denotes	,
T6781	22373-22376	DT	denotes	the
T6782	22377-22387	NN	denotes	efficiency
T6783	22388-22390	IN	denotes	of
T6784	22391-22394	DT	denotes	the
T6785	22395-22407	NN	denotes	displacement
T6786	22408-22421	RB	denotes	significantly
T6787	22432-22434	IN	denotes	as
T6788	22470-22477	VBD	denotes	dropped
T6789	22435-22438	DT	denotes	the
T6790	22447-22454	NN	denotes	content
T6791	22439-22446	NN	denotes	thymine
T6792	22455-22457	IN	denotes	of
T6793	22458-22461	DT	denotes	the
T6794	22465-22469	NN	denotes	tail
T6795	22462-22463	CD	denotes	3
T6796	22463-22464	SYM	denotes	′
T6797	22464-22465	HYPH	denotes	-
T6798	22478-22480	IN	denotes	to
T6799	22481-22483	CD	denotes	33
T6800	22483-22484	NN	denotes	%
T6801	22484-22486	,	denotes	,
T6802	22486-22488	IN	denotes	as
T6803	22489-22491	IN	denotes	in
T6804	22492-22497	NN	denotes	TAA17
T6805	22498-22500	CC	denotes	or
T6806	22501-22506	NN	denotes	TCC17
T6807	22507-22508	-LRB-	denotes	(
T6808	22515-22517	NN	denotes	6B
T6809	22508-22514	NN	denotes	Figure
T6810	22518-22521	CC	denotes	and
T6811	22522-22523	NN	denotes	C
T6812	22523-22524	-RRB-	denotes	)
T6813	22524-22525	.	denotes	.
T6814	22525-22640	sentence	denotes	Thus, the minimal thymine content required for activation of the Mcm helicase may be somewhere between 33 and 50%.
T6815	22526-22530	RB	denotes	Thus
T6816	22608-22610	VB	denotes	be
T6817	22530-22532	,	denotes	,
T6818	22532-22535	DT	denotes	the
T6819	22552-22559	NN	denotes	content
T6820	22536-22543	JJ	denotes	minimal
T6821	22544-22551	NN	denotes	thymine
T6822	22560-22568	VBN	denotes	required
T6823	22569-22572	IN	denotes	for
T6824	22573-22583	NN	denotes	activation
T6825	22584-22586	IN	denotes	of
T6826	22587-22590	DT	denotes	the
T6827	22595-22603	NN	denotes	helicase
T6828	22591-22594	NN	denotes	Mcm
T6829	22604-22607	MD	denotes	may
T6830	22611-22620	RB	denotes	somewhere
T6831	22621-22628	IN	denotes	between
T6832	22629-22631	CD	denotes	33
T6833	22638-22639	NN	denotes	%
T6834	22632-22635	CC	denotes	and
T6835	22636-22638	CD	denotes	50
T6836	22639-22640	.	denotes	.
T7008	22642-22645	NN	denotes	DUE
T7009	22646-22655	NNS	denotes	sequences
T7010	22678-22686	VBP	denotes	activate
T7011	22656-22660	IN	denotes	from
T7012	22661-22664	DT	denotes	the
T7013	22671-22677	NN	denotes	origin
T7014	22665-22666	NN	denotes	c
T7015	22667-22670	NN	denotes	myc
T7016	22666-22667	HYPH	denotes	-
T7017	22687-22690	NN	denotes	Mcm
T7018	22691-22699	NN	denotes	helicase
T7019	22699-22999	sentence	denotes	Our previous studies have shown that the sequences containing periodic (dT)n tracts derived from the human lamin B2 origin (48% thymine content) can serve as an efficient activator for the Mcm helicase on a bubble, and replacement of thymines with guanines abolished the helicase activation (13,28).
T7020	22700-22703	PRP$	denotes	Our
T7021	22713-22720	NNS	denotes	studies
T7022	22704-22712	JJ	denotes	previous
T7023	22726-22731	VBN	denotes	shown
T7024	22721-22725	VBP	denotes	have
T7025	22732-22736	IN	denotes	that
T7026	22849-22854	VB	denotes	serve
T7027	22737-22740	DT	denotes	the
T7028	22741-22750	NNS	denotes	sequences
T7029	22751-22761	VBG	denotes	containing
T7030	22762-22770	JJ	denotes	periodic
T7031	22777-22783	NNS	denotes	tracts
T7032	22771-22772	-LRB-	denotes	(
T7033	22775-22776	NN	denotes	n
T7034	22772-22774	NN	denotes	dT
T7035	22774-22775	-RRB-	denotes	)
T7036	22784-22791	VBN	denotes	derived
T7037	22792-22796	IN	denotes	from
T7038	22797-22800	DT	denotes	the
T7039	22816-22822	NN	denotes	origin
T7040	22801-22806	JJ	denotes	human
T7041	22807-22812	NN	denotes	lamin
T7042	22813-22815	NN	denotes	B2
T7043	22823-22824	-LRB-	denotes	(
T7044	22836-22843	NN	denotes	content
T7045	22824-22826	CD	denotes	48
T7046	22826-22827	NN	denotes	%
T7047	22828-22835	NN	denotes	thymine
T7048	22843-22844	-RRB-	denotes	)
T7049	22845-22848	MD	denotes	can
T7050	22855-22857	IN	denotes	as
T7051	22858-22860	DT	denotes	an
T7052	22871-22880	NN	denotes	activator
T7053	22861-22870	JJ	denotes	efficient
T7054	22881-22884	IN	denotes	for
T7055	22885-22888	DT	denotes	the
T7056	22893-22901	NN	denotes	helicase
T7057	22889-22892	NN	denotes	Mcm
T7058	22902-22904	IN	denotes	on
T7059	22905-22906	DT	denotes	a
T7060	22907-22913	NN	denotes	bubble
T7061	22913-22915	,	denotes	,
T7062	22915-22918	CC	denotes	and
T7063	22919-22930	NN	denotes	replacement
T7064	22957-22966	VBD	denotes	abolished
T7065	22931-22933	IN	denotes	of
T7066	22934-22942	NNS	denotes	thymines
T7067	22943-22947	IN	denotes	with
T7068	22948-22956	NNS	denotes	guanines
T7069	22967-22970	DT	denotes	the
T7070	22980-22990	NN	denotes	activation
T7071	22971-22979	NN	denotes	helicase
T7072	22991-22992	-LRB-	denotes	(
T7073	22995-22997	CD	denotes	28
T7074	22992-22994	CD	denotes	13
T7075	22994-22995	,	denotes	,
T7076	22997-22998	-RRB-	denotes	)
T7077	22998-22999	.	denotes	.
T7078	22999-23357	sentence	denotes	In order to examine other natural replication origins with different thymine contents for the ability to activate Mcm helicase on a bubble substrate, we constructed new bubble substrates, Bub82/c-myc, containing sequences derived from the DUE (DNA unwinding element) region of the human c-myc origin which is essential for c-myc replicator activity (29,30).
T7079	23000-23002	IN	denotes	In
T7080	23153-23164	VBD	denotes	constructed
T7081	23003-23008	NN	denotes	order
T7082	23009-23011	TO	denotes	to
T7083	23012-23019	VB	denotes	examine
T7084	23020-23025	JJ	denotes	other
T7085	23046-23053	NNS	denotes	origins
T7086	23026-23033	JJ	denotes	natural
T7087	23034-23045	NN	denotes	replication
T7088	23054-23058	IN	denotes	with
T7089	23059-23068	JJ	denotes	different
T7090	23077-23085	NNS	denotes	contents
T7091	23069-23076	NN	denotes	thymine
T7092	23086-23089	IN	denotes	for
T7093	23090-23093	DT	denotes	the
T7094	23094-23101	NN	denotes	ability
T7095	23102-23104	TO	denotes	to
T7096	23105-23113	VB	denotes	activate
T7097	23114-23117	NN	denotes	Mcm
T7098	23118-23126	NN	denotes	helicase
T7099	23127-23129	IN	denotes	on
T7100	23130-23131	DT	denotes	a
T7101	23139-23148	NN	denotes	substrate
T7102	23132-23138	NN	denotes	bubble
T7103	23148-23150	,	denotes	,
T7104	23150-23152	PRP	denotes	we
T7105	23165-23168	JJ	denotes	new
T7106	23176-23186	NNS	denotes	substrates
T7107	23169-23175	NN	denotes	bubble
T7108	23186-23188	,	denotes	,
T7109	23188-23193	NN	denotes	Bub82
T7110	23196-23199	NN	denotes	myc
T7111	23193-23194	HYPH	denotes	/
T7112	23194-23195	NN	denotes	c
T7113	23195-23196	HYPH	denotes	-
T7114	23199-23201	,	denotes	,
T7115	23201-23211	VBG	denotes	containing
T7116	23212-23221	NNS	denotes	sequences
T7117	23222-23229	VBN	denotes	derived
T7118	23230-23234	IN	denotes	from
T7119	23235-23238	DT	denotes	the
T7120	23267-23273	NN	denotes	region
T7121	23239-23242	NN	denotes	DUE
T7122	23243-23244	-LRB-	denotes	(
T7123	23244-23247	NN	denotes	DNA
T7124	23258-23265	NN	denotes	element
T7125	23248-23257	NN	denotes	unwinding
T7126	23265-23266	-RRB-	denotes	)
T7127	23274-23276	IN	denotes	of
T7128	23277-23280	DT	denotes	the
T7129	23293-23299	NN	denotes	origin
T7130	23281-23286	JJ	denotes	human
T7131	23287-23288	NN	denotes	c
T7132	23289-23292	NN	denotes	myc
T7133	23288-23289	HYPH	denotes	-
T7134	23300-23305	WDT	denotes	which
T7135	23306-23308	VBZ	denotes	is
T7136	23309-23318	JJ	denotes	essential
T7137	23319-23322	IN	denotes	for
T7138	23323-23324	NN	denotes	c
T7139	23325-23328	NN	denotes	myc
T7140	23324-23325	HYPH	denotes	-
T7141	23340-23348	NN	denotes	activity
T7142	23329-23339	NN	denotes	replicator
T7143	23349-23350	-LRB-	denotes	(
T7144	23353-23355	CD	denotes	30
T7145	23350-23352	CD	denotes	29
T7146	23352-23353	,	denotes	,
T7147	23355-23356	-RRB-	denotes	)
T7148	23356-23357	.	denotes	.
T7149	23357-23479	sentence	denotes	The unpaired segment in Bub82/c-myc/DUE contains one strand of DUE and that in Bub82/c-myc/DUE-C contains another strand.
T7150	23358-23361	DT	denotes	The
T7151	23371-23378	NN	denotes	segment
T7152	23362-23370	JJ	denotes	unpaired
T7153	23398-23406	VBZ	denotes	contains
T7154	23379-23381	IN	denotes	in
T7155	23382-23387	NN	denotes	Bub82
T7156	23394-23397	NN	denotes	DUE
T7157	23387-23388	HYPH	denotes	/
T7158	23388-23389	NN	denotes	c
T7159	23390-23393	NN	denotes	myc
T7160	23389-23390	HYPH	denotes	-
T7161	23393-23394	HYPH	denotes	/
T7162	23407-23410	CD	denotes	one
T7163	23411-23417	NN	denotes	strand
T7164	23418-23420	IN	denotes	of
T7165	23421-23424	NN	denotes	DUE
T7166	23425-23428	CC	denotes	and
T7167	23429-23433	DT	denotes	that
T7168	23455-23463	VBZ	denotes	contains
T7169	23434-23436	IN	denotes	in
T7170	23437-23442	NN	denotes	Bub82
T7171	23453-23454	NN	denotes	C
T7172	23442-23443	HYPH	denotes	/
T7173	23443-23444	NN	denotes	c
T7174	23445-23448	NN	denotes	myc
T7175	23444-23445	HYPH	denotes	-
T7176	23448-23449	HYPH	denotes	/
T7177	23449-23452	NN	denotes	DUE
T7178	23452-23453	HYPH	denotes	-
T7179	23464-23471	DT	denotes	another
T7180	23472-23478	NN	denotes	strand
T7181	23478-23479	.	denotes	.
T7182	23479-23641	sentence	denotes	This DUE is believed to coincide with the initially unwound region of the c-myc origin (29), and its deletion substantially reduced the replicator activity (30).
T7183	23480-23484	DT	denotes	This
T7184	23485-23488	NN	denotes	DUE
T7185	23492-23500	VBN	denotes	believed
T7186	23489-23491	VBZ	denotes	is
T7187	23501-23503	TO	denotes	to
T7188	23504-23512	VB	denotes	coincide
T7189	23513-23517	IN	denotes	with
T7190	23518-23521	DT	denotes	the
T7191	23540-23546	NN	denotes	region
T7192	23522-23531	RB	denotes	initially
T7193	23532-23539	JJ	denotes	unwound
T7194	23547-23549	IN	denotes	of
T7195	23550-23553	DT	denotes	the
T7196	23560-23566	NN	denotes	origin
T7197	23554-23555	NN	denotes	c
T7198	23556-23559	NN	denotes	myc
T7199	23555-23556	HYPH	denotes	-
T7200	23567-23568	-LRB-	denotes	(
T7201	23568-23570	CD	denotes	29
T7202	23570-23571	-RRB-	denotes	)
T7203	23571-23573	,	denotes	,
T7204	23573-23576	CC	denotes	and
T7205	23577-23580	PRP$	denotes	its
T7206	23581-23589	NN	denotes	deletion
T7207	23604-23611	VBD	denotes	reduced
T7208	23590-23603	RB	denotes	substantially
T7209	23612-23615	DT	denotes	the
T7210	23627-23635	NN	denotes	activity
T7211	23616-23626	NN	denotes	replicator
T7212	23636-23637	-LRB-	denotes	(
T7213	23637-23639	CD	denotes	30
T7214	23639-23640	-RRB-	denotes	)
T7215	23640-23641	.	denotes	.
T7216	23641-23887	sentence	denotes	Mcm4/6/7 unwound Bub82/lamin B2 (thymine 48%), Bub82/c-myc/DUE (37%) and Bub82/c-myc/DUE-C (39%) with similar efficiency (Figure 7A), indicating that the sequences from the c-myc origin also can serve as efficient activators of the Mcm helicase.
T7217	23642-23646	NN	denotes	Mcm4
T7218	23651-23658	VBN	denotes	unwound
T7219	23646-23647	HYPH	denotes	/
T7220	23647-23648	CD	denotes	6
T7221	23648-23649	HYPH	denotes	/
T7222	23649-23650	CD	denotes	7
T7223	23659-23664	NN	denotes	Bub82
T7224	23671-23673	NN	denotes	B2
T7225	23664-23665	HYPH	denotes	/
T7226	23665-23670	NN	denotes	lamin
T7227	23674-23675	-LRB-	denotes	(
T7228	23685-23686	NN	denotes	%
T7229	23675-23682	NN	denotes	thymine
T7230	23683-23685	CD	denotes	48
T7231	23686-23687	-RRB-	denotes	)
T7232	23687-23689	,	denotes	,
T7233	23689-23694	NN	denotes	Bub82
T7234	23701-23704	NN	denotes	DUE
T7235	23694-23695	HYPH	denotes	/
T7236	23695-23696	NN	denotes	c
T7237	23697-23700	NN	denotes	myc
T7238	23696-23697	HYPH	denotes	-
T7239	23700-23701	HYPH	denotes	/
T7240	23705-23706	-LRB-	denotes	(
T7241	23708-23709	NN	denotes	%
T7242	23706-23708	CD	denotes	37
T7243	23709-23710	-RRB-	denotes	)
T7244	23711-23714	CC	denotes	and
T7245	23715-23720	NN	denotes	Bub82
T7246	23731-23732	NN	denotes	C
T7247	23720-23721	HYPH	denotes	/
T7248	23721-23722	NN	denotes	c
T7249	23723-23726	NN	denotes	myc
T7250	23722-23723	HYPH	denotes	-
T7251	23726-23727	HYPH	denotes	/
T7252	23727-23730	NN	denotes	DUE
T7253	23730-23731	HYPH	denotes	-
T7254	23733-23734	-LRB-	denotes	(
T7255	23736-23737	NN	denotes	%
T7256	23734-23736	CD	denotes	39
T7257	23737-23738	-RRB-	denotes	)
T7258	23739-23743	IN	denotes	with
T7259	23744-23751	JJ	denotes	similar
T7260	23752-23762	NN	denotes	efficiency
T7261	23763-23764	-LRB-	denotes	(
T7262	23771-23773	NN	denotes	7A
T7263	23764-23770	NN	denotes	Figure
T7264	23773-23774	-RRB-	denotes	)
T7265	23774-23776	,	denotes	,
T7266	23776-23786	VBG	denotes	indicating
T7267	23787-23791	IN	denotes	that
T7268	23837-23842	VB	denotes	serve
T7269	23792-23795	DT	denotes	the
T7270	23796-23805	NNS	denotes	sequences
T7271	23806-23810	IN	denotes	from
T7272	23811-23814	DT	denotes	the
T7273	23821-23827	NN	denotes	origin
T7274	23815-23816	NN	denotes	c
T7275	23817-23820	NN	denotes	myc
T7276	23816-23817	HYPH	denotes	-
T7277	23828-23832	RB	denotes	also
T7278	23833-23836	MD	denotes	can
T7279	23843-23845	IN	denotes	as
T7280	23846-23855	JJ	denotes	efficient
T7281	23856-23866	NNS	denotes	activators
T7282	23867-23869	IN	denotes	of
T7283	23870-23873	DT	denotes	the
T7284	23878-23886	NN	denotes	helicase
T7285	23874-23877	NN	denotes	Mcm
T7286	23886-23887	.	denotes	.
T7287	23887-24153	sentence	denotes	This result also indicates that sequences with 37% thymine content can activate Mcm4/6/7 to displace 24 nt duplex on both sides in a bubble substrate, suggesting that the not only thymine content but also the sequence context may affect the Mcm helicase activation.
T7288	23888-23892	DT	denotes	This
T7289	23893-23899	NN	denotes	result
T7290	23905-23914	VBZ	denotes	indicates
T7291	23900-23904	RB	denotes	also
T7292	23915-23919	IN	denotes	that
T7293	23959-23967	VB	denotes	activate
T7294	23920-23929	NNS	denotes	sequences
T7295	23930-23934	IN	denotes	with
T7296	23935-23937	CD	denotes	37
T7297	23937-23938	NN	denotes	%
T7298	23947-23954	NN	denotes	content
T7299	23939-23946	NN	denotes	thymine
T7300	23955-23958	MD	denotes	can
T7301	23968-23972	NN	denotes	Mcm4
T7302	23972-23973	HYPH	denotes	/
T7303	23973-23974	CD	denotes	6
T7304	23974-23975	HYPH	denotes	/
T7305	23975-23976	CD	denotes	7
T7306	23977-23979	TO	denotes	to
T7307	23980-23988	VB	denotes	displace
T7308	23989-23991	CD	denotes	24
T7309	23992-23994	NN	denotes	nt
T7310	23995-24001	NN	denotes	duplex
T7311	24002-24004	IN	denotes	on
T7312	24005-24009	DT	denotes	both
T7313	24010-24015	NNS	denotes	sides
T7314	24016-24018	IN	denotes	in
T7315	24019-24020	DT	denotes	a
T7316	24028-24037	NN	denotes	substrate
T7317	24021-24027	NN	denotes	bubble
T7318	24037-24039	,	denotes	,
T7319	24039-24049	VBG	denotes	suggesting
T7320	24050-24054	IN	denotes	that
T7321	24118-24124	VB	denotes	affect
T7322	24055-24058	DT	denotes	the
T7323	24076-24083	NN	denotes	content
T7324	24059-24062	RB	denotes	not
T7325	24063-24067	RB	denotes	only
T7326	24068-24075	NN	denotes	thymine
T7327	24084-24087	CC	denotes	but
T7328	24088-24092	RB	denotes	also
T7329	24093-24096	DT	denotes	the
T7330	24106-24113	NN	denotes	context
T7331	24097-24105	NN	denotes	sequence
T7332	24114-24117	MD	denotes	may
T7333	24125-24128	DT	denotes	the
T7334	24142-24152	NN	denotes	activation
T7335	24129-24132	NN	denotes	Mcm
T7336	24133-24141	NN	denotes	helicase
T7337	24152-24153	.	denotes	.
T7338	24153-24341	sentence	denotes	In gel shift assays, MCM4/6/7 bound to all the three bubbles, although the affinity to Bub82/c-myc/DUE and to Bub82/c-myc/DUE-C is slightly lower than that to Bub-82/lamin B2 (Figure 7B).
T7339	24154-24156	IN	denotes	In
T7340	24184-24189	VBD	denotes	bound
T7341	24157-24160	NN	denotes	gel
T7342	24161-24166	NN	denotes	shift
T7343	24167-24173	NNS	denotes	assays
T7344	24173-24175	,	denotes	,
T7345	24175-24179	NN	denotes	MCM4
T7346	24179-24180	HYPH	denotes	/
T7347	24180-24181	CD	denotes	6
T7348	24181-24182	HYPH	denotes	/
T7349	24182-24183	CD	denotes	7
T7350	24190-24192	IN	denotes	to
T7351	24193-24196	PDT	denotes	all
T7352	24207-24214	NNS	denotes	bubbles
T7353	24197-24200	DT	denotes	the
T7354	24201-24206	CD	denotes	three
T7355	24214-24216	,	denotes	,
T7356	24216-24224	IN	denotes	although
T7357	24282-24284	VBZ	denotes	is
T7358	24225-24228	DT	denotes	the
T7359	24229-24237	NN	denotes	affinity
T7360	24238-24240	IN	denotes	to
T7361	24241-24246	NN	denotes	Bub82
T7362	24253-24256	NN	denotes	DUE
T7363	24246-24247	HYPH	denotes	/
T7364	24247-24248	NN	denotes	c
T7365	24249-24252	NN	denotes	myc
T7366	24248-24249	HYPH	denotes	-
T7367	24252-24253	HYPH	denotes	/
T7368	24257-24260	CC	denotes	and
T7369	24261-24263	IN	denotes	to
T7370	24264-24269	NN	denotes	Bub82
T7371	24280-24281	NN	denotes	C
T7372	24269-24270	HYPH	denotes	/
T7373	24270-24271	NN	denotes	c
T7374	24272-24275	NN	denotes	myc
T7375	24271-24272	HYPH	denotes	-
T7376	24275-24276	HYPH	denotes	/
T7377	24276-24279	NN	denotes	DUE
T7378	24279-24280	HYPH	denotes	-
T7379	24285-24293	RB	denotes	slightly
T7380	24294-24299	JJR	denotes	lower
T7381	24300-24304	IN	denotes	than
T7382	24305-24309	DT	denotes	that
T7383	24310-24312	IN	denotes	to
T7384	24313-24316	NN	denotes	Bub
T7385	24326-24328	NN	denotes	B2
T7386	24316-24317	HYPH	denotes	-
T7387	24317-24319	CD	denotes	82
T7388	24319-24320	HYPH	denotes	/
T7389	24320-24325	NN	denotes	lamin
T7390	24329-24330	-LRB-	denotes	(
T7391	24337-24339	NN	denotes	7B
T7392	24330-24336	NN	denotes	Figure
T7393	24339-24340	-RRB-	denotes	)
T7394	24340-24341	.	denotes	.
T7395	24341-24434	sentence	denotes	Therefore, helicase and DNA binding activities do not necessarily correlate with each other.
T7396	24342-24351	RB	denotes	Therefore
T7397	24408-24417	VB	denotes	correlate
T7398	24351-24353	,	denotes	,
T7399	24353-24361	NN	denotes	helicase
T7400	24378-24388	NNS	denotes	activities
T7401	24362-24365	CC	denotes	and
T7402	24366-24369	NN	denotes	DNA
T7403	24370-24377	NN	denotes	binding
T7404	24389-24391	VBP	denotes	do
T7405	24392-24395	RB	denotes	not
T7406	24396-24407	RB	denotes	necessarily
T7407	24418-24422	IN	denotes	with
T7408	24423-24427	DT	denotes	each
T7409	24428-24433	JJ	denotes	other
T7410	24433-24434	.	denotes	.
T7411	24434-24573	sentence	denotes	This was also indicated by our previous results that guanine and cytosine stretches can bind to Mcm but cannot activate the helicase (13).
T7412	24435-24439	DT	denotes	This
T7413	24449-24458	VBN	denotes	indicated
T7414	24440-24443	VBD	denotes	was
T7415	24444-24448	RB	denotes	also
T7416	24459-24461	IN	denotes	by
T7417	24462-24465	PRP$	denotes	our
T7418	24475-24482	NNS	denotes	results
T7419	24466-24474	JJ	denotes	previous
T7420	24483-24487	IN	denotes	that
T7421	24523-24527	VB	denotes	bind
T7422	24488-24495	NN	denotes	guanine
T7423	24509-24518	NNS	denotes	stretches
T7424	24496-24499	CC	denotes	and
T7425	24500-24508	NN	denotes	cytosine
T7426	24519-24522	MD	denotes	can
T7427	24528-24530	IN	denotes	to
T7428	24531-24534	NN	denotes	Mcm
T7429	24535-24538	CC	denotes	but
T7430	24539-24542	MD	denotes	can
T7431	24546-24554	VB	denotes	activate
T7432	24542-24545	RB	denotes	not
T7433	24555-24558	DT	denotes	the
T7434	24559-24567	NN	denotes	helicase
T7435	24568-24569	-LRB-	denotes	(
T7436	24569-24571	CD	denotes	13
T7437	24571-24572	-RRB-	denotes	)
T7438	24572-24573	.	denotes	.
T7722	24575-24578	NN	denotes	DNA
T7723	24579-24588	NN	denotes	unwinding
T7724	24604-24613	VBN	denotes	inhibited
T7725	24589-24591	IN	denotes	by
T7726	24592-24596	NN	denotes	Mcm4
T7727	24596-24597	HYPH	denotes	/
T7728	24597-24598	CD	denotes	6
T7729	24598-24599	HYPH	denotes	/
T7730	24599-24600	CD	denotes	7
T7731	24601-24603	VBZ	denotes	is
T7732	24614-24616	IN	denotes	by
T7733	24617-24620	DT	denotes	the
T7734	24629-24638	NNS	denotes	sequences
T7735	24621-24623	NN	denotes	GC
T7736	24624-24628	JJ	denotes	rich
T7737	24623-24624	HYPH	denotes	-
T7738	24639-24641	IN	denotes	on
T7739	24642-24645	DT	denotes	the
T7740	24653-24660	NN	denotes	segment
T7741	24646-24652	NN	denotes	duplex
T7742	24660-24787	sentence	denotes	Our results indicate that thymine-rich single-stranded DNA is required for initial loading and activation of the Mcm helicase.
T7743	24661-24664	PRP$	denotes	Our
T7744	24665-24672	NNS	denotes	results
T7745	24673-24681	VBP	denotes	indicate
T7746	24682-24686	IN	denotes	that
T7747	24723-24731	VBN	denotes	required
T7748	24687-24694	NN	denotes	thymine
T7749	24695-24699	JJ	denotes	rich
T7750	24694-24695	HYPH	denotes	-
T7751	24716-24719	NN	denotes	DNA
T7752	24700-24706	JJ	denotes	single
T7753	24707-24715	VBN	denotes	stranded
T7754	24706-24707	HYPH	denotes	-
T7755	24720-24722	VBZ	denotes	is
T7756	24732-24735	IN	denotes	for
T7757	24736-24743	JJ	denotes	initial
T7758	24744-24751	NN	denotes	loading
T7759	24752-24755	CC	denotes	and
T7760	24756-24766	NN	denotes	activation
T7761	24767-24769	IN	denotes	of
T7762	24770-24773	DT	denotes	the
T7763	24778-24786	NN	denotes	helicase
T7764	24774-24777	NN	denotes	Mcm
T7765	24786-24787	.	denotes	.
T7766	24787-24891	sentence	denotes	However, it is not known whether thymine sequences are required for processive unwinding of duplex DNA.
T7767	24788-24795	RB	denotes	However
T7768	24807-24812	VBN	denotes	known
T7769	24795-24797	,	denotes	,
T7770	24797-24799	PRP	denotes	it
T7771	24800-24802	VBZ	denotes	is
T7772	24803-24806	RB	denotes	not
T7773	24813-24820	IN	denotes	whether
T7774	24843-24851	VBN	denotes	required
T7775	24821-24828	NN	denotes	thymine
T7776	24829-24838	NNS	denotes	sequences
T7777	24839-24842	VBP	denotes	are
T7778	24852-24855	IN	denotes	for
T7779	24856-24866	JJ	denotes	processive
T7780	24867-24876	NN	denotes	unwinding
T7781	24877-24879	IN	denotes	of
T7782	24880-24886	NN	denotes	duplex
T7783	24887-24890	NN	denotes	DNA
T7784	24890-24891	.	denotes	.
T7785	24891-25007	sentence	denotes	Therefore, we have examined whether the nucleotide composition of the duplex region affects its unwinding activity.
T7786	24892-24901	RB	denotes	Therefore
T7787	24911-24919	VBN	denotes	examined
T7788	24901-24903	,	denotes	,
T7789	24903-24905	PRP	denotes	we
T7790	24906-24910	VBP	denotes	have
T7791	24920-24927	IN	denotes	whether
T7792	24976-24983	VBZ	denotes	affects
T7793	24928-24931	DT	denotes	the
T7794	24943-24954	NN	denotes	composition
T7795	24932-24942	NN	denotes	nucleotide
T7796	24955-24957	IN	denotes	of
T7797	24958-24961	DT	denotes	the
T7798	24969-24975	NN	denotes	region
T7799	24962-24968	NN	denotes	duplex
T7800	24984-24987	PRP$	denotes	its
T7801	24998-25006	NN	denotes	activity
T7802	24988-24997	NN	denotes	unwinding
T7803	25006-25007	.	denotes	.
T7804	25007-25145	sentence	denotes	To address this issue, we constructed a series of T-tailed Y-fork structures (T-fork) containing various sequences in the duplex segment.
T7805	25008-25010	TO	denotes	To
T7806	25011-25018	VB	denotes	address
T7807	25034-25045	VBD	denotes	constructed
T7808	25019-25023	DT	denotes	this
T7809	25024-25029	NN	denotes	issue
T7810	25029-25031	,	denotes	,
T7811	25031-25033	PRP	denotes	we
T7812	25046-25047	DT	denotes	a
T7813	25048-25054	NN	denotes	series
T7814	25055-25057	IN	denotes	of
T7815	25058-25059	NN	denotes	T
T7816	25060-25066	VBN	denotes	tailed
T7817	25059-25060	HYPH	denotes	-
T7818	25074-25084	NNS	denotes	structures
T7819	25067-25068	NN	denotes	Y
T7820	25069-25073	NN	denotes	fork
T7821	25068-25069	HYPH	denotes	-
T7822	25085-25086	-LRB-	denotes	(
T7823	25086-25087	NN	denotes	T
T7824	25088-25092	NN	denotes	fork
T7825	25087-25088	HYPH	denotes	-
T7826	25092-25093	-RRB-	denotes	)
T7827	25094-25104	VBG	denotes	containing
T7828	25105-25112	JJ	denotes	various
T7829	25113-25122	NNS	denotes	sequences
T7830	25123-25125	IN	denotes	in
T7831	25126-25129	DT	denotes	the
T7832	25137-25144	NN	denotes	segment
T7833	25130-25136	NN	denotes	duplex
T7834	25144-25145	.	denotes	.
T7835	25145-25218	sentence	denotes	They carried varied contents of cytosine residues on the 3′-tail strand.
T7836	25146-25150	PRP	denotes	They
T7837	25151-25158	VBD	denotes	carried
T7838	25159-25165	VBN	denotes	varied
T7839	25166-25174	NNS	denotes	contents
T7840	25175-25177	IN	denotes	of
T7841	25178-25186	NN	denotes	cytosine
T7842	25187-25195	NNS	denotes	residues
T7843	25196-25198	IN	denotes	on
T7844	25199-25202	DT	denotes	the
T7845	25211-25217	NN	denotes	strand
T7846	25203-25204	CD	denotes	3
T7847	25206-25210	NN	denotes	tail
T7848	25204-25205	SYM	denotes	′
T7849	25205-25206	HYPH	denotes	-
T7850	25217-25218	.	denotes	.
T7851	25218-25395	sentence	denotes	In gel shift assays, Mcm4/6/7 bound to these Y-fork substrates with identical affinity (Figure 8A), consistent with the notion that the Mcm4/6/7 binds to single-stranded tails.
T7852	25219-25221	IN	denotes	In
T7853	25249-25254	VBD	denotes	bound
T7854	25222-25225	NN	denotes	gel
T7855	25226-25231	NN	denotes	shift
T7856	25232-25238	NNS	denotes	assays
T7857	25238-25240	,	denotes	,
T7858	25240-25244	NN	denotes	Mcm4
T7859	25244-25245	HYPH	denotes	/
T7860	25245-25246	CD	denotes	6
T7861	25246-25247	HYPH	denotes	/
T7862	25247-25248	CD	denotes	7
T7863	25255-25257	IN	denotes	to
T7864	25258-25263	DT	denotes	these
T7865	25271-25281	NNS	denotes	substrates
T7866	25264-25265	NN	denotes	Y
T7867	25266-25270	NN	denotes	fork
T7868	25265-25266	HYPH	denotes	-
T7869	25282-25286	IN	denotes	with
T7870	25287-25296	JJ	denotes	identical
T7871	25297-25305	NN	denotes	affinity
T7872	25306-25307	-LRB-	denotes	(
T7873	25314-25316	NN	denotes	8A
T7874	25307-25313	NN	denotes	Figure
T7875	25316-25317	-RRB-	denotes	)
T7876	25317-25319	,	denotes	,
T7877	25319-25329	JJ	denotes	consistent
T7878	25330-25334	IN	denotes	with
T7879	25335-25338	DT	denotes	the
T7880	25339-25345	NN	denotes	notion
T7881	25346-25350	IN	denotes	that
T7882	25364-25369	VBZ	denotes	binds
T7883	25351-25354	DT	denotes	the
T7884	25355-25359	NN	denotes	Mcm4
T7885	25359-25360	HYPH	denotes	/
T7886	25360-25361	CD	denotes	6
T7887	25361-25362	HYPH	denotes	/
T7888	25362-25363	CD	denotes	7
T7889	25370-25372	IN	denotes	to
T7890	25373-25379	JJ	denotes	single
T7891	25380-25388	VBN	denotes	stranded
T7892	25379-25380	HYPH	denotes	-
T7893	25389-25394	NNS	denotes	tails
T7894	25394-25395	.	denotes	.
T7895	25395-25547	sentence	denotes	However, in DNA helicase assays, T-fork/(C:G)49 was hardly displaced by Mcm4/6/7, but was readily displaced by SV40 T-antigen DNA helicase (Figure 8B).
T7896	25396-25403	RB	denotes	However
T7897	25455-25464	VBN	denotes	displaced
T7898	25403-25405	,	denotes	,
T7899	25405-25407	IN	denotes	in
T7900	25408-25411	NN	denotes	DNA
T7901	25412-25420	NN	denotes	helicase
T7902	25421-25427	NNS	denotes	assays
T7903	25427-25429	,	denotes	,
T7904	25429-25430	NN	denotes	T
T7905	25431-25435	NN	denotes	fork
T7906	25430-25431	HYPH	denotes	-
T7907	25435-25436	HYPH	denotes	/
T7908	25436-25437	-LRB-	denotes	(
T7909	25439-25440	NN	denotes	G
T7910	25437-25438	NN	denotes	C
T7911	25438-25439	:	denotes	:
T7912	25440-25441	-RRB-	denotes	)
T7913	25441-25443	CD	denotes	49
T7914	25444-25447	VBD	denotes	was
T7915	25448-25454	RB	denotes	hardly
T7916	25465-25467	IN	denotes	by
T7917	25468-25472	NN	denotes	Mcm4
T7918	25472-25473	HYPH	denotes	/
T7919	25473-25474	CD	denotes	6
T7920	25474-25475	HYPH	denotes	/
T7921	25475-25476	CD	denotes	7
T7922	25476-25478	,	denotes	,
T7923	25478-25481	CC	denotes	but
T7924	25482-25485	VBD	denotes	was
T7925	25494-25503	VBN	denotes	displaced
T7926	25486-25493	RB	denotes	readily
T7927	25504-25506	IN	denotes	by
T7928	25507-25511	NN	denotes	SV40
T7929	25526-25534	NN	denotes	helicase
T7930	25512-25513	NN	denotes	T
T7931	25514-25521	NN	denotes	antigen
T7932	25513-25514	HYPH	denotes	-
T7933	25522-25525	NN	denotes	DNA
T7934	25535-25536	-LRB-	denotes	(
T7935	25543-25545	NN	denotes	8B
T7936	25536-25542	NN	denotes	Figure
T7937	25545-25546	-RRB-	denotes	)
T7938	25546-25547	.	denotes	.
T7939	25547-25713	sentence	denotes	When thymine or adenine is inserted as every third nucleotide (repeats of CCT or CCA), the extent of unwinding increased (T-fork/(CCT:GGA)16 and T-fork/(CCA:GGT)16).
T7940	25548-25552	WRB	denotes	When
T7941	25575-25583	VBN	denotes	inserted
T7942	25553-25560	NN	denotes	thymine
T7943	25561-25563	CC	denotes	or
T7944	25564-25571	NN	denotes	adenine
T7945	25572-25574	VBZ	denotes	is
T7946	25659-25668	VBD	denotes	increased
T7947	25584-25586	IN	denotes	as
T7948	25587-25592	DT	denotes	every
T7949	25599-25609	NN	denotes	nucleotide
T7950	25593-25598	JJ	denotes	third
T7951	25610-25611	-LRB-	denotes	(
T7952	25611-25618	NNS	denotes	repeats
T7953	25619-25621	IN	denotes	of
T7954	25622-25625	NN	denotes	CCT
T7955	25626-25628	CC	denotes	or
T7956	25629-25632	NN	denotes	CCA
T7957	25632-25633	-RRB-	denotes	)
T7958	25633-25635	,	denotes	,
T7959	25635-25638	DT	denotes	the
T7960	25639-25645	NN	denotes	extent
T7961	25646-25648	IN	denotes	of
T7962	25649-25658	NN	denotes	unwinding
T7963	25669-25670	-LRB-	denotes	(
T7964	25682-25685	NN	denotes	GGA
T7965	25670-25671	NN	denotes	T
T7966	25672-25676	NN	denotes	fork
T7967	25671-25672	HYPH	denotes	-
T7968	25676-25677	HYPH	denotes	/
T7969	25677-25678	-LRB-	denotes	(
T7970	25678-25681	NN	denotes	CCT
T7971	25681-25682	:	denotes	:
T7972	25685-25686	-RRB-	denotes	)
T7973	25686-25688	CD	denotes	16
T7974	25689-25692	CC	denotes	and
T7975	25693-25694	NN	denotes	T
T7976	25695-25699	NN	denotes	fork
T7977	25694-25695	HYPH	denotes	-
T7978	25705-25708	NN	denotes	GGT
T7979	25699-25700	HYPH	denotes	/
T7980	25700-25701	-LRB-	denotes	(
T7981	25701-25704	NN	denotes	CCA
T7982	25704-25705	:	denotes	:
T7983	25708-25709	-RRB-	denotes	)
T7984	25709-25711	CD	denotes	16
T7985	25711-25712	-RRB-	denotes	)
T7986	25712-25713	.	denotes	.
T7987	25713-25918	sentence	denotes	The efficiency of unwinding is roughly correlated with the content of GC pairs in the duplex segment [T-fork/(CCAA:GGTT)12, T-fork/(CCTT:GGAA)12, T-fork/(CAAA:GTTT)12 and T-fork/(CTTT:GAAA)12; Figure 8B].
T7988	25714-25717	DT	denotes	The
T7989	25718-25728	NN	denotes	efficiency
T7990	25753-25763	VBN	denotes	correlated
T7991	25729-25731	IN	denotes	of
T7992	25732-25741	NN	denotes	unwinding
T7993	25742-25744	VBZ	denotes	is
T7994	25745-25752	RB	denotes	roughly
T7995	25764-25768	IN	denotes	with
T7996	25769-25772	DT	denotes	the
T7997	25773-25780	NN	denotes	content
T7998	25781-25783	IN	denotes	of
T7999	25784-25786	NN	denotes	GC
T8000	25787-25792	NNS	denotes	pairs
T8001	25793-25795	IN	denotes	in
T8002	25796-25799	DT	denotes	the
T8003	25807-25814	NN	denotes	segment
T8004	25800-25806	NN	denotes	duplex
T8005	25815-25816	-LRB-	denotes	[
T8006	25829-25833	NN	denotes	GGTT
T8007	25816-25817	NN	denotes	T
T8008	25818-25822	NN	denotes	fork
T8009	25817-25818	HYPH	denotes	-
T8010	25822-25823	HYPH	denotes	/
T8011	25823-25824	-LRB-	denotes	(
T8012	25824-25828	NN	denotes	CCAA
T8013	25828-25829	:	denotes	:
T8014	25833-25834	-RRB-	denotes	)
T8015	25834-25836	CD	denotes	12
T8016	25836-25838	,	denotes	,
T8017	25838-25839	NN	denotes	T
T8018	25840-25844	NN	denotes	fork
T8019	25839-25840	HYPH	denotes	-
T8020	25851-25855	NN	denotes	GGAA
T8021	25844-25845	HYPH	denotes	/
T8022	25845-25846	-LRB-	denotes	(
T8023	25846-25850	NN	denotes	CCTT
T8024	25850-25851	:	denotes	:
T8025	25855-25856	-RRB-	denotes	)
T8026	25856-25858	CD	denotes	12
T8027	25858-25860	,	denotes	,
T8028	25860-25861	NN	denotes	T
T8029	25862-25866	NN	denotes	fork
T8030	25861-25862	HYPH	denotes	-
T8031	25873-25877	NN	denotes	GTTT
T8032	25866-25867	HYPH	denotes	/
T8033	25867-25868	-LRB-	denotes	(
T8034	25868-25872	NN	denotes	CAAA
T8035	25872-25873	:	denotes	:
T8036	25877-25878	-RRB-	denotes	)
T8037	25878-25880	CD	denotes	12
T8038	25881-25884	CC	denotes	and
T8039	25885-25886	NN	denotes	T
T8040	25887-25891	NN	denotes	fork
T8041	25886-25887	HYPH	denotes	-
T8042	25898-25902	NN	denotes	GAAA
T8043	25891-25892	HYPH	denotes	/
T8044	25892-25893	-LRB-	denotes	(
T8045	25893-25897	NN	denotes	CTTT
T8046	25897-25898	:	denotes	:
T8047	25902-25903	-RRB-	denotes	)
T8048	25903-25905	CD	denotes	12
T8049	25905-25906	:	denotes	;
T8050	25914-25916	NN	denotes	8B
T8051	25907-25913	NN	denotes	Figure
T8052	25916-25917	-RRB-	denotes	]
T8053	25917-25918	.	denotes	.
T8054	25918-26014	sentence	denotes	It appears that the duplex segment containing <50% GC pairs is efficiently unwound (Figure 8C).
T8055	25919-25921	PRP	denotes	It
T8056	25922-25929	VBZ	denotes	appears
T8057	25930-25934	IN	denotes	that
T8058	25994-26001	VBN	denotes	unwound
T8059	25935-25938	DT	denotes	the
T8060	25946-25953	NN	denotes	segment
T8061	25939-25945	NN	denotes	duplex
T8062	25954-25964	VBG	denotes	containing
T8063	25965-25966	SYM	denotes	<
T8064	25966-25968	CD	denotes	50
T8065	25968-25969	NN	denotes	%
T8066	25973-25978	NNS	denotes	pairs
T8067	25970-25972	NN	denotes	GC
T8068	25979-25981	VBZ	denotes	is
T8069	25982-25993	RB	denotes	efficiently
T8070	26002-26003	-LRB-	denotes	(
T8071	26010-26012	NN	denotes	8C
T8072	26003-26009	NN	denotes	Figure
T8073	26012-26013	-RRB-	denotes	)
T8074	26013-26014	.	denotes	.
T8075	26014-26127	sentence	denotes	We next examined the effect of GC-rich segments on a partial heteroduplex DNA on a single-stranded circular DNA.
T8076	26015-26017	PRP	denotes	We
T8077	26023-26031	VBD	denotes	examined
T8078	26018-26022	RB	denotes	next
T8079	26032-26035	DT	denotes	the
T8080	26036-26042	NN	denotes	effect
T8081	26043-26045	IN	denotes	of
T8082	26046-26048	NN	denotes	GC
T8083	26049-26053	JJ	denotes	rich
T8084	26048-26049	HYPH	denotes	-
T8085	26054-26062	NNS	denotes	segments
T8086	26063-26065	IN	denotes	on
T8087	26066-26067	DT	denotes	a
T8088	26089-26092	NN	denotes	DNA
T8089	26068-26075	JJ	denotes	partial
T8090	26076-26088	NN	denotes	heteroduplex
T8091	26093-26095	IN	denotes	on
T8092	26096-26097	DT	denotes	a
T8093	26123-26126	NN	denotes	DNA
T8094	26098-26104	JJ	denotes	single
T8095	26105-26113	VBN	denotes	stranded
T8096	26104-26105	HYPH	denotes	-
T8097	26114-26122	JJ	denotes	circular
T8098	26126-26127	.	denotes	.
T8099	26127-26385	sentence	denotes	We have constructed two sets of 5′-tailed partial heteroduplex DNA substrates containing duplex regions of variable lengths; one on M13mp18 vector and the other on M13mp19 containing a 66 nt long G-rich segment downstream of the hybridizing oligonucleotide.
T8100	26128-26130	PRP	denotes	We
T8101	26136-26147	VBN	denotes	constructed
T8102	26131-26135	VBP	denotes	have
T8103	26148-26151	CD	denotes	two
T8104	26152-26156	NNS	denotes	sets
T8105	26157-26159	IN	denotes	of
T8106	26160-26161	CD	denotes	5
T8107	26163-26169	VBN	denotes	tailed
T8108	26161-26162	SYM	denotes	′
T8109	26162-26163	HYPH	denotes	-
T8110	26195-26205	NNS	denotes	substrates
T8111	26170-26177	JJ	denotes	partial
T8112	26178-26190	NN	denotes	heteroduplex
T8113	26191-26194	NN	denotes	DNA
T8114	26206-26216	VBG	denotes	containing
T8115	26217-26223	NN	denotes	duplex
T8116	26224-26231	NNS	denotes	regions
T8117	26232-26234	IN	denotes	of
T8118	26235-26243	JJ	denotes	variable
T8119	26244-26251	NNS	denotes	lengths
T8120	26251-26252	:	denotes	;
T8121	26253-26256	CD	denotes	one
T8122	26257-26259	IN	denotes	on
T8123	26260-26267	NN	denotes	M13mp18
T8124	26268-26274	NN	denotes	vector
T8125	26275-26278	CC	denotes	and
T8126	26279-26282	DT	denotes	the
T8127	26283-26288	JJ	denotes	other
T8128	26289-26291	IN	denotes	on
T8129	26292-26299	NN	denotes	M13mp19
T8130	26300-26310	VBG	denotes	containing
T8131	26311-26312	DT	denotes	a
T8132	26331-26338	NN	denotes	segment
T8133	26313-26315	CD	denotes	66
T8134	26316-26318	NN	denotes	nt
T8135	26319-26323	JJ	denotes	long
T8136	26324-26325	NN	denotes	G
T8137	26326-26330	JJ	denotes	rich
T8138	26325-26326	HYPH	denotes	-
T8139	26339-26349	RB	denotes	downstream
T8140	26350-26352	IN	denotes	of
T8141	26353-26356	DT	denotes	the
T8142	26369-26384	NN	denotes	oligonucleotide
T8143	26357-26368	VBG	denotes	hybridizing
T8144	26384-26385	.	denotes	.
T8145	26385-26556	sentence	denotes	We found that the Mcm4/6/7 helicase can displace duplex DNA up to 350 nt long on its own on the former substrate (Figure 8D, left), consistent with previous results (13).
T8146	26386-26388	PRP	denotes	We
T8147	26389-26394	VBD	denotes	found
T8148	26395-26399	IN	denotes	that
T8149	26426-26434	VB	denotes	displace
T8150	26400-26403	DT	denotes	the
T8151	26413-26421	NN	denotes	helicase
T8152	26404-26408	NN	denotes	Mcm4
T8153	26408-26409	HYPH	denotes	/
T8154	26409-26410	CD	denotes	6
T8155	26410-26411	HYPH	denotes	/
T8156	26411-26412	CD	denotes	7
T8157	26422-26425	MD	denotes	can
T8158	26435-26441	NN	denotes	duplex
T8159	26442-26445	NN	denotes	DNA
T8160	26446-26448	IN	denotes	up
T8161	26452-26455	CD	denotes	350
T8162	26449-26451	IN	denotes	to
T8163	26456-26458	NN	denotes	nt
T8164	26459-26463	JJ	denotes	long
T8165	26464-26466	IN	denotes	on
T8166	26467-26470	PRP$	denotes	its
T8167	26471-26474	JJ	denotes	own
T8168	26475-26477	IN	denotes	on
T8169	26478-26481	DT	denotes	the
T8170	26489-26498	NN	denotes	substrate
T8171	26482-26488	JJ	denotes	former
T8172	26499-26500	-LRB-	denotes	(
T8173	26507-26509	NN	denotes	8D
T8174	26500-26506	NN	denotes	Figure
T8175	26509-26511	,	denotes	,
T8176	26511-26515	JJ	denotes	left
T8177	26515-26516	-RRB-	denotes	)
T8178	26516-26518	,	denotes	,
T8179	26518-26528	JJ	denotes	consistent
T8180	26529-26533	IN	denotes	with
T8181	26534-26542	JJ	denotes	previous
T8182	26543-26550	NNS	denotes	results
T8183	26551-26552	-LRB-	denotes	(
T8184	26552-26554	CD	denotes	13
T8185	26554-26555	-RRB-	denotes	)
T8186	26555-26556	.	denotes	.
T8187	26556-26803	sentence	denotes	In contrast, displacement was inhibited over the GC-rich segments on the latter substrate (Figure 8D, right panel), although displacement up to 250 nt in length, albeit at a reduced level, was observed at a high concentration of Mcm4/6/7 complex.
T8188	26557-26559	IN	denotes	In
T8189	26587-26596	VBN	denotes	inhibited
T8190	26560-26568	NN	denotes	contrast
T8191	26568-26570	,	denotes	,
T8192	26570-26582	NN	denotes	displacement
T8193	26583-26586	VBD	denotes	was
T8194	26597-26601	IN	denotes	over
T8195	26602-26605	DT	denotes	the
T8196	26614-26622	NNS	denotes	segments
T8197	26606-26608	NN	denotes	GC
T8198	26609-26613	JJ	denotes	rich
T8199	26608-26609	HYPH	denotes	-
T8200	26623-26625	IN	denotes	on
T8201	26626-26629	DT	denotes	the
T8202	26637-26646	NN	denotes	substrate
T8203	26630-26636	JJ	denotes	latter
T8204	26647-26648	-LRB-	denotes	(
T8205	26665-26670	NN	denotes	panel
T8206	26648-26654	NN	denotes	Figure
T8207	26655-26657	NN	denotes	8D
T8208	26657-26659	,	denotes	,
T8209	26659-26664	JJ	denotes	right
T8210	26670-26671	-RRB-	denotes	)
T8211	26671-26673	,	denotes	,
T8212	26673-26681	IN	denotes	although
T8213	26750-26758	VBN	denotes	observed
T8214	26682-26694	NN	denotes	displacement
T8215	26695-26697	IN	denotes	up
T8216	26701-26704	CD	denotes	250
T8217	26698-26700	IN	denotes	to
T8218	26705-26707	NN	denotes	nt
T8219	26708-26710	IN	denotes	in
T8220	26711-26717	NN	denotes	length
T8221	26717-26719	,	denotes	,
T8222	26719-26725	IN	denotes	albeit
T8223	26726-26728	IN	denotes	at
T8224	26729-26730	DT	denotes	a
T8225	26739-26744	NN	denotes	level
T8226	26731-26738	VBN	denotes	reduced
T8227	26744-26746	,	denotes	,
T8228	26746-26749	VBD	denotes	was
T8229	26759-26761	IN	denotes	at
T8230	26762-26763	DT	denotes	a
T8231	26769-26782	NN	denotes	concentration
T8232	26764-26768	JJ	denotes	high
T8233	26783-26785	IN	denotes	of
T8234	26786-26790	NN	denotes	Mcm4
T8235	26795-26802	NN	denotes	complex
T8236	26790-26791	HYPH	denotes	/
T8237	26791-26792	CD	denotes	6
T8238	26792-26793	HYPH	denotes	/
T8239	26793-26794	CD	denotes	7
T8240	26802-26803	.	denotes	.
T8241	26803-26940	sentence	denotes	This result is consistent with that on Y-fork and indicates that GC-rich duplex segment is inhibitory for unwinding by the Mcm helicase.
T8242	26804-26808	DT	denotes	This
T8243	26809-26815	NN	denotes	result
T8244	26816-26818	VBZ	denotes	is
T8245	26819-26829	JJ	denotes	consistent
T8246	26830-26834	IN	denotes	with
T8247	26835-26839	DT	denotes	that
T8248	26840-26842	IN	denotes	on
T8249	26843-26844	NN	denotes	Y
T8250	26845-26849	NN	denotes	fork
T8251	26844-26845	HYPH	denotes	-
T8252	26850-26853	CC	denotes	and
T8253	26854-26863	VBZ	denotes	indicates
T8254	26864-26868	IN	denotes	that
T8255	26892-26894	VBZ	denotes	is
T8256	26869-26871	NN	denotes	GC
T8257	26872-26876	JJ	denotes	rich
T8258	26871-26872	HYPH	denotes	-
T8259	26884-26891	NN	denotes	segment
T8260	26877-26883	NN	denotes	duplex
T8261	26895-26905	JJ	denotes	inhibitory
T8262	26906-26909	IN	denotes	for
T8263	26910-26919	NN	denotes	unwinding
T8264	26920-26922	IN	denotes	by
T8265	26923-26926	DT	denotes	the
T8266	26931-26939	NN	denotes	helicase
T8267	26927-26930	NN	denotes	Mcm
T8268	26939-26940	.	denotes	.
T8269	26940-27105	sentence	denotes	These results may indicate that the presence of thymine–adenine pairs with a certain frequency in the duplex region may facilitate continuous unwinding by Mcm4/6/7.
T8270	26941-26946	DT	denotes	These
T8271	26947-26954	NNS	denotes	results
T8272	26959-26967	VB	denotes	indicate
T8273	26955-26958	MD	denotes	may
T8274	26968-26972	IN	denotes	that
T8275	27061-27071	VB	denotes	facilitate
T8276	26973-26976	DT	denotes	the
T8277	26977-26985	NN	denotes	presence
T8278	26986-26988	IN	denotes	of
T8279	26989-26996	NN	denotes	thymine
T8280	26997-27004	NN	denotes	adenine
T8281	26996-26997	HYPH	denotes	–
T8282	27005-27010	NNS	denotes	pairs
T8283	27011-27015	IN	denotes	with
T8284	27016-27017	DT	denotes	a
T8285	27026-27035	NN	denotes	frequency
T8286	27018-27025	JJ	denotes	certain
T8287	27036-27038	IN	denotes	in
T8288	27039-27042	DT	denotes	the
T8289	27050-27056	NN	denotes	region
T8290	27043-27049	NN	denotes	duplex
T8291	27057-27060	MD	denotes	may
T8292	27072-27082	JJ	denotes	continuous
T8293	27083-27092	NN	denotes	unwinding
T8294	27093-27095	IN	denotes	by
T8295	27096-27100	NN	denotes	Mcm4
T8296	27100-27101	HYPH	denotes	/
T8297	27101-27102	CD	denotes	6
T8298	27102-27103	HYPH	denotes	/
T8299	27103-27104	CD	denotes	7
T8300	27104-27105	.	denotes	.
T8301	27105-27214	sentence	denotes	Alternatively, Mcm4/6/7 is not efficient enough to displace thermodynamically stable GC-base-paired segment.
T8302	27106-27119	RB	denotes	Alternatively
T8303	27130-27132	VBZ	denotes	is
T8304	27119-27121	,	denotes	,
T8305	27121-27125	NN	denotes	Mcm4
T8306	27125-27126	HYPH	denotes	/
T8307	27126-27127	CD	denotes	6
T8308	27127-27128	HYPH	denotes	/
T8309	27128-27129	CD	denotes	7
T8310	27133-27136	RB	denotes	not
T8311	27137-27146	JJ	denotes	efficient
T8312	27147-27153	RB	denotes	enough
T8313	27154-27156	TO	denotes	to
T8314	27157-27165	VB	denotes	displace
T8315	27166-27183	RB	denotes	thermodynamically
T8316	27184-27190	JJ	denotes	stable
T8317	27206-27213	NN	denotes	segment
T8318	27191-27193	NN	denotes	GC
T8319	27199-27205	VBN	denotes	paired
T8320	27193-27194	HYPH	denotes	-
T8321	27194-27198	NN	denotes	base
T8322	27198-27199	HYPH	denotes	-
T8323	27213-27214	.	denotes	.
T8324	27214-27359	sentence	denotes	In order to distinguish these two possibilities, we designed a new T-fork substrate containing inosine (I) residue instead of guanosine residue.
T8325	27215-27217	IN	denotes	In
T8326	27267-27275	VBD	denotes	designed
T8327	27218-27223	NN	denotes	order
T8328	27224-27226	TO	denotes	to
T8329	27227-27238	VB	denotes	distinguish
T8330	27239-27244	DT	denotes	these
T8331	27249-27262	NNS	denotes	possibilities
T8332	27245-27248	CD	denotes	two
T8333	27262-27264	,	denotes	,
T8334	27264-27266	PRP	denotes	we
T8335	27276-27277	DT	denotes	a
T8336	27289-27298	NN	denotes	substrate
T8337	27278-27281	JJ	denotes	new
T8338	27282-27283	NN	denotes	T
T8339	27284-27288	NN	denotes	fork
T8340	27283-27284	HYPH	denotes	-
T8341	27299-27309	VBG	denotes	containing
T8342	27310-27317	NN	denotes	inosine
T8343	27322-27329	NN	denotes	residue
T8344	27318-27319	-LRB-	denotes	(
T8345	27319-27320	NN	denotes	I
T8346	27320-27321	-RRB-	denotes	)
T8347	27330-27337	RB	denotes	instead
T8348	27338-27340	IN	denotes	of
T8349	27341-27350	NN	denotes	guanosine
T8350	27351-27358	NN	denotes	residue
T8351	27358-27359	.	denotes	.
T8352	27359-27532	sentence	denotes	The thermal stability of the I:C base pair is lower than that of the G:C base pair in duplex DNA due to loss of one hydrogen bond and is even lower than A:T base pair (31).
T8353	27360-27363	DT	denotes	The
T8354	27372-27381	NN	denotes	stability
T8355	27364-27371	JJ	denotes	thermal
T8356	27403-27405	VBZ	denotes	is
T8357	27382-27384	IN	denotes	of
T8358	27385-27388	DT	denotes	the
T8359	27398-27402	NN	denotes	pair
T8360	27389-27390	NN	denotes	I
T8361	27391-27392	NN	denotes	C
T8362	27390-27391	:	denotes	:
T8363	27393-27397	NN	denotes	base
T8364	27406-27411	JJR	denotes	lower
T8365	27412-27416	IN	denotes	than
T8366	27417-27421	DT	denotes	that
T8367	27422-27424	IN	denotes	of
T8368	27425-27428	DT	denotes	the
T8369	27438-27442	NN	denotes	pair
T8370	27429-27430	NN	denotes	G
T8371	27431-27432	NN	denotes	C
T8372	27430-27431	:	denotes	:
T8373	27433-27437	NN	denotes	base
T8374	27443-27445	IN	denotes	in
T8375	27446-27452	NN	denotes	duplex
T8376	27453-27456	NN	denotes	DNA
T8377	27457-27460	IN	denotes	due
T8378	27461-27463	IN	denotes	to
T8379	27464-27468	NN	denotes	loss
T8380	27469-27471	IN	denotes	of
T8381	27472-27475	CD	denotes	one
T8382	27485-27489	NN	denotes	bond
T8383	27476-27484	NN	denotes	hydrogen
T8384	27490-27493	CC	denotes	and
T8385	27494-27496	VBZ	denotes	is
T8386	27497-27501	RB	denotes	even
T8387	27502-27507	JJR	denotes	lower
T8388	27508-27512	IN	denotes	than
T8389	27513-27514	NN	denotes	A
T8390	27515-27516	NN	denotes	T
T8391	27514-27515	:	denotes	:
T8392	27522-27526	NN	denotes	pair
T8393	27517-27521	NN	denotes	base
T8394	27527-27528	-LRB-	denotes	(
T8395	27528-27530	CD	denotes	31
T8396	27530-27531	-RRB-	denotes	)
T8397	27531-27532	.	denotes	.
T8398	27532-27663	sentence	denotes	We constructed the T-tailed fork substrates containing the (GCC:CGG)10, (GCC:CIG)10 or (GAA:CTT)10 duplex DNA segment (Figure 8E).
T8399	27533-27535	PRP	denotes	We
T8400	27536-27547	VBD	denotes	constructed
T8401	27548-27551	DT	denotes	the
T8402	27566-27576	NNS	denotes	substrates
T8403	27552-27553	NN	denotes	T
T8404	27554-27560	VBN	denotes	tailed
T8405	27553-27554	HYPH	denotes	-
T8406	27561-27565	NN	denotes	fork
T8407	27577-27587	VBG	denotes	containing
T8408	27588-27591	DT	denotes	the
T8409	27643-27650	NN	denotes	segment
T8410	27592-27593	-LRB-	denotes	(
T8411	27597-27600	NN	denotes	CGG
T8412	27593-27596	NN	denotes	GCC
T8413	27596-27597	:	denotes	:
T8414	27600-27601	-RRB-	denotes	)
T8415	27601-27603	CD	denotes	10
T8416	27603-27605	,	denotes	,
T8417	27605-27606	-LRB-	denotes	(
T8418	27610-27613	NN	denotes	CIG
T8419	27606-27609	NN	denotes	GCC
T8420	27609-27610	:	denotes	:
T8421	27613-27614	-RRB-	denotes	)
T8422	27614-27616	CD	denotes	10
T8423	27617-27619	CC	denotes	or
T8424	27620-27621	-LRB-	denotes	(
T8425	27625-27628	NN	denotes	CTT
T8426	27621-27624	NN	denotes	GAA
T8427	27624-27625	:	denotes	:
T8428	27628-27629	-RRB-	denotes	)
T8429	27629-27631	CD	denotes	10
T8430	27632-27638	NN	denotes	duplex
T8431	27639-27642	NN	denotes	DNA
T8432	27651-27652	-LRB-	denotes	(
T8433	27659-27661	NN	denotes	8E
T8434	27652-27658	NN	denotes	Figure
T8435	27661-27662	-RRB-	denotes	)
T8436	27662-27663	.	denotes	.
T8437	27663-27809	sentence	denotes	Mcm4/6/7 hardly displaced the 31 nt long duplex of GCC:CGG repeats, whereas the T-fork/(GAA:CTT)10 was displaced efficiently, as described above.
T8438	27664-27668	NN	denotes	Mcm4
T8439	27680-27689	VBD	denotes	displaced
T8440	27668-27669	HYPH	denotes	/
T8441	27669-27670	CD	denotes	6
T8442	27670-27671	HYPH	denotes	/
T8443	27671-27672	CD	denotes	7
T8444	27673-27679	RB	denotes	hardly
T8445	27690-27693	DT	denotes	the
T8446	27705-27711	NN	denotes	duplex
T8447	27694-27696	CD	denotes	31
T8448	27697-27699	NN	denotes	nt
T8449	27700-27704	JJ	denotes	long
T8450	27712-27714	IN	denotes	of
T8451	27715-27718	NN	denotes	GCC
T8452	27719-27722	NN	denotes	CGG
T8453	27718-27719	:	denotes	:
T8454	27723-27730	NNS	denotes	repeats
T8455	27730-27732	,	denotes	,
T8456	27732-27739	IN	denotes	whereas
T8457	27767-27776	VBN	denotes	displaced
T8458	27740-27743	DT	denotes	the
T8459	27756-27759	NN	denotes	CTT
T8460	27744-27745	NN	denotes	T
T8461	27746-27750	NN	denotes	fork
T8462	27745-27746	HYPH	denotes	-
T8463	27750-27751	HYPH	denotes	/
T8464	27751-27752	-LRB-	denotes	(
T8465	27752-27755	NN	denotes	GAA
T8466	27755-27756	:	denotes	:
T8467	27759-27760	-RRB-	denotes	)
T8468	27760-27762	CD	denotes	10
T8469	27763-27766	VBD	denotes	was
T8470	27777-27788	RB	denotes	efficiently
T8471	27788-27790	,	denotes	,
T8472	27790-27792	IN	denotes	as
T8473	27793-27802	VBN	denotes	described
T8474	27803-27808	RB	denotes	above
T8475	27808-27809	.	denotes	.
T8476	27809-28058	sentence	denotes	T-fork/(GCC:CIG)10 was displaced with efficiency much greater than that of T-fork/(GCC:CGG)10, indicating that the thermostability of the duplex segment, not the lack of AT base pair, is responsible for inability of Mcm4/6/7 to displace the duplex.
T8477	27810-27811	NN	denotes	T
T8478	27812-27816	NN	denotes	fork
T8479	27811-27812	HYPH	denotes	-
T8480	27822-27825	NN	denotes	CIG
T8481	27816-27817	HYPH	denotes	/
T8482	27817-27818	-LRB-	denotes	(
T8483	27818-27821	NN	denotes	GCC
T8484	27821-27822	:	denotes	:
T8485	27833-27842	VBN	denotes	displaced
T8486	27825-27826	-RRB-	denotes	)
T8487	27826-27828	CD	denotes	10
T8488	27829-27832	VBD	denotes	was
T8489	27843-27847	IN	denotes	with
T8490	27848-27858	NN	denotes	efficiency
T8491	27859-27863	RB	denotes	much
T8492	27864-27871	JJR	denotes	greater
T8493	27872-27876	IN	denotes	than
T8494	27877-27881	DT	denotes	that
T8495	27882-27884	IN	denotes	of
T8496	27885-27886	NN	denotes	T
T8497	27887-27891	NN	denotes	fork
T8498	27886-27887	HYPH	denotes	-
T8499	27897-27900	NN	denotes	CGG
T8500	27891-27892	HYPH	denotes	/
T8501	27892-27893	-LRB-	denotes	(
T8502	27893-27896	NN	denotes	GCC
T8503	27896-27897	:	denotes	:
T8504	27900-27901	-RRB-	denotes	)
T8505	27901-27903	CD	denotes	10
T8506	27903-27905	,	denotes	,
T8507	27905-27915	VBG	denotes	indicating
T8508	27916-27920	IN	denotes	that
T8509	27994-27996	VBZ	denotes	is
T8510	27921-27924	DT	denotes	the
T8511	27925-27940	NN	denotes	thermostability
T8512	27941-27943	IN	denotes	of
T8513	27944-27947	DT	denotes	the
T8514	27955-27962	NN	denotes	segment
T8515	27948-27954	NN	denotes	duplex
T8516	27962-27964	,	denotes	,
T8517	27964-27967	CC	denotes	not
T8518	27968-27971	DT	denotes	the
T8519	27972-27976	NN	denotes	lack
T8520	27977-27979	IN	denotes	of
T8521	27980-27982	NN	denotes	AT
T8522	27988-27992	NN	denotes	pair
T8523	27983-27987	NN	denotes	base
T8524	27992-27994	,	denotes	,
T8525	27997-28008	JJ	denotes	responsible
T8526	28009-28012	IN	denotes	for
T8527	28013-28022	NN	denotes	inability
T8528	28023-28025	IN	denotes	of
T8529	28026-28030	NN	denotes	Mcm4
T8530	28030-28031	HYPH	denotes	/
T8531	28031-28032	CD	denotes	6
T8532	28032-28033	HYPH	denotes	/
T8533	28033-28034	CD	denotes	7
T8534	28035-28037	TO	denotes	to
T8535	28038-28046	VB	denotes	displace
T8536	28047-28050	DT	denotes	the
T8537	28051-28057	NN	denotes	duplex
T8538	28057-28058	.	denotes	.
T8838	28338-28345	JJ	denotes	crucial
T8839	28346-28349	IN	denotes	for
T8840	28350-28363	VBG	denotes	understanding
T8841	28364-28367	DT	denotes	the
T8842	28378-28383	NN	denotes	basis
T8843	28368-28377	JJ	denotes	molecular
T8844	28384-28386	IN	denotes	of
T8845	28387-28397	NN	denotes	initiation
T8846	28398-28400	IN	denotes	of
T8847	28401-28404	NN	denotes	DNA
T8848	28405-28416	NN	denotes	replication
T8849	28416-28417	.	denotes	.
T8850	28417-28506	sentence	denotes	It is now believed that Mcm is a major component of the eukaryotic replicative helicase.
T8851	28418-28420	PRP	denotes	It
T8852	28428-28436	VBN	denotes	believed
T8853	28421-28423	VBZ	denotes	is
T8854	28424-28427	RB	denotes	now
T8855	28437-28441	IN	denotes	that
T8856	28446-28448	VBZ	denotes	is
T8857	28442-28445	NN	denotes	Mcm
T8858	28449-28450	DT	denotes	a
T8859	28457-28466	NN	denotes	component
T8860	28451-28456	JJ	denotes	major
T8861	28467-28469	IN	denotes	of
T8862	28470-28473	DT	denotes	the
T8863	28497-28505	NN	denotes	helicase
T8864	28474-28484	JJ	denotes	eukaryotic
T8865	28485-28496	JJ	denotes	replicative
T8866	28505-28506	.	denotes	.
T8867	28506-28671	sentence	denotes	In this report, we have conducted detailed analyses on binding and helicase actions of mouse Mcm4/6/7 using various substrates including fork and bubble structures.
T8868	28507-28509	IN	denotes	In
T8869	28531-28540	VBN	denotes	conducted
T8870	28510-28514	DT	denotes	this
T8871	28515-28521	NN	denotes	report
T8872	28521-28523	,	denotes	,
T8873	28523-28525	PRP	denotes	we
T8874	28526-28530	VBP	denotes	have
T8875	28541-28549	JJ	denotes	detailed
T8876	28550-28558	NNS	denotes	analyses
T8877	28559-28561	IN	denotes	on
T8878	28562-28569	NN	denotes	binding
T8879	28583-28590	NNS	denotes	actions
T8880	28570-28573	CC	denotes	and
T8881	28574-28582	NN	denotes	helicase
T8882	28591-28593	IN	denotes	of
T8883	28594-28599	NN	denotes	mouse
T8884	28600-28604	NN	denotes	Mcm4
T8885	28604-28605	HYPH	denotes	/
T8886	28605-28606	CD	denotes	6
T8887	28606-28607	HYPH	denotes	/
T8888	28607-28608	CD	denotes	7
T8889	28609-28614	VBG	denotes	using
T8890	28615-28622	JJ	denotes	various
T8891	28623-28633	NNS	denotes	substrates
T8892	28634-28643	VBG	denotes	including
T8893	28644-28648	NN	denotes	fork
T8894	28660-28670	NNS	denotes	structures
T8895	28649-28652	CC	denotes	and
T8896	28653-28659	NN	denotes	bubble
T8897	28670-28671	.	denotes	.
T9088	28673-28678	NNS	denotes	Modes
T9089	28679-28681	IN	denotes	of
T9090	28682-28689	NN	denotes	binding
T9091	28690-28693	CC	denotes	and
T9092	28694-28704	NN	denotes	activation
T9093	28705-28707	IN	denotes	of
T9094	28708-28712	NN	denotes	Mcm4
T9095	28717-28725	NN	denotes	helicase
T9096	28712-28713	HYPH	denotes	/
T9097	28713-28714	CD	denotes	6
T9098	28714-28715	HYPH	denotes	/
T9099	28715-28716	CD	denotes	7
T9100	28726-28728	IN	denotes	on
T9101	28729-28730	DT	denotes	a
T9102	28738-28747	NN	denotes	structure
T9103	28731-28737	NN	denotes	bubble
T9104	28747-28854	sentence	denotes	Initiation of DNA replication is associated with localized melting of duplex DNA near replication origins.
T9105	28748-28758	NN	denotes	Initiation
T9106	28781-28791	VBN	denotes	associated
T9107	28759-28761	IN	denotes	of
T9108	28762-28765	NN	denotes	DNA
T9109	28766-28777	NN	denotes	replication
T9110	28778-28780	VBZ	denotes	is
T9111	28792-28796	IN	denotes	with
T9112	28797-28806	VBN	denotes	localized
T9113	28807-28814	NN	denotes	melting
T9114	28815-28817	IN	denotes	of
T9115	28818-28824	NN	denotes	duplex
T9116	28825-28828	NN	denotes	DNA
T9117	28829-28833	JJ	denotes	near
T9118	28834-28845	NN	denotes	replication
T9119	28846-28853	NNS	denotes	origins
T9120	28853-28854	.	denotes	.
T9121	28854-28973	sentence	denotes	Helicases are loaded onto replication forks through the melted region, induced by initiator binding, in bacteria (32).
T9122	28855-28864	NNS	denotes	Helicases
T9123	28869-28875	VBN	denotes	loaded
T9124	28865-28868	VBP	denotes	are
T9125	28876-28880	IN	denotes	onto
T9126	28881-28892	NN	denotes	replication
T9127	28893-28898	NNS	denotes	forks
T9128	28899-28906	IN	denotes	through
T9129	28907-28910	DT	denotes	the
T9130	28918-28924	NN	denotes	region
T9131	28911-28917	VBN	denotes	melted
T9132	28924-28926	,	denotes	,
T9133	28926-28933	VBN	denotes	induced
T9134	28934-28936	IN	denotes	by
T9135	28937-28946	NN	denotes	initiator
T9136	28947-28954	NN	denotes	binding
T9137	28954-28956	,	denotes	,
T9138	28956-28958	IN	denotes	in
T9139	28959-28967	NNS	denotes	bacteria
T9140	28968-28969	-LRB-	denotes	(
T9141	28969-28971	CD	denotes	32
T9142	28971-28972	-RRB-	denotes	)
T9143	28972-28973	.	denotes	.
T9144	28973-29104	sentence	denotes	We previously reported that Mcm4/6/7 can be loaded onto a bubble-like structure and can serve as a DNA helicase at the forks (13).
T9145	28974-28976	PRP	denotes	We
T9146	28988-28996	VBD	denotes	reported
T9147	28977-28987	RB	denotes	previously
T9148	28997-29001	IN	denotes	that
T9149	29018-29024	VBN	denotes	loaded
T9150	29002-29006	NN	denotes	Mcm4
T9151	29006-29007	HYPH	denotes	/
T9152	29007-29008	CD	denotes	6
T9153	29008-29009	HYPH	denotes	/
T9154	29009-29010	CD	denotes	7
T9155	29011-29014	MD	denotes	can
T9156	29015-29017	VB	denotes	be
T9157	29025-29029	IN	denotes	onto
T9158	29030-29031	DT	denotes	a
T9159	29044-29053	NN	denotes	structure
T9160	29032-29038	NN	denotes	bubble
T9161	29039-29043	JJ	denotes	like
T9162	29038-29039	HYPH	denotes	-
T9163	29054-29057	CC	denotes	and
T9164	29058-29061	MD	denotes	can
T9165	29062-29067	VB	denotes	serve
T9166	29068-29070	IN	denotes	as
T9167	29071-29072	DT	denotes	a
T9168	29077-29085	NN	denotes	helicase
T9169	29073-29076	NN	denotes	DNA
T9170	29086-29088	IN	denotes	at
T9171	29089-29092	DT	denotes	the
T9172	29093-29098	NNS	denotes	forks
T9173	29099-29100	-LRB-	denotes	(
T9174	29100-29102	CD	denotes	13
T9175	29102-29103	-RRB-	denotes	)
T9176	29103-29104	.	denotes	.
T9177	29104-29298	sentence	denotes	The ability of Mcm4/6/7 to unwind the bubble substrate but not the conventional duplex DNA (Figures 2 and 13) indicates that Mcm can be loaded through the single-stranded segment of the bubble.
T9178	29105-29108	DT	denotes	The
T9179	29109-29116	NN	denotes	ability
T9180	29215-29224	VBZ	denotes	indicates
T9181	29117-29119	IN	denotes	of
T9182	29120-29124	NN	denotes	Mcm4
T9183	29124-29125	HYPH	denotes	/
T9184	29125-29126	CD	denotes	6
T9185	29126-29127	HYPH	denotes	/
T9186	29127-29128	CD	denotes	7
T9187	29129-29131	TO	denotes	to
T9188	29132-29138	VB	denotes	unwind
T9189	29139-29142	DT	denotes	the
T9190	29150-29159	NN	denotes	substrate
T9191	29143-29149	NN	denotes	bubble
T9192	29160-29163	CC	denotes	but
T9193	29164-29167	RB	denotes	not
T9194	29168-29171	DT	denotes	the
T9195	29192-29195	NN	denotes	DNA
T9196	29172-29184	JJ	denotes	conventional
T9197	29185-29191	NN	denotes	duplex
T9198	29196-29197	-LRB-	denotes	(
T9199	29205-29206	CD	denotes	2
T9200	29197-29204	NNS	denotes	Figures
T9201	29207-29210	CC	denotes	and
T9202	29211-29213	CD	denotes	13
T9203	29213-29214	-RRB-	denotes	)
T9204	29225-29229	IN	denotes	that
T9205	29241-29247	VBN	denotes	loaded
T9206	29230-29233	NN	denotes	Mcm
T9207	29234-29237	MD	denotes	can
T9208	29238-29240	VB	denotes	be
T9209	29248-29255	IN	denotes	through
T9210	29256-29259	DT	denotes	the
T9211	29276-29283	NN	denotes	segment
T9212	29260-29266	JJ	denotes	single
T9213	29267-29275	VBN	denotes	stranded
T9214	29266-29267	HYPH	denotes	-
T9215	29284-29286	IN	denotes	of
T9216	29287-29290	DT	denotes	the
T9217	29291-29297	NN	denotes	bubble
T9218	29297-29298	.	denotes	.
T9219	29298-29451	sentence	denotes	Furthermore, the Mcm4/6/7 complex displays marked preference for thymine-rich sequences within the single-stranded segment for helicase activation (13).
T9220	29299-29310	RB	denotes	Furthermore
T9221	29333-29341	VBZ	denotes	displays
T9222	29310-29312	,	denotes	,
T9223	29312-29315	DT	denotes	the
T9224	29325-29332	NN	denotes	complex
T9225	29316-29320	NN	denotes	Mcm4
T9226	29320-29321	HYPH	denotes	/
T9227	29321-29322	CD	denotes	6
T9228	29322-29323	HYPH	denotes	/
T9229	29323-29324	CD	denotes	7
T9230	29342-29348	JJ	denotes	marked
T9231	29349-29359	NN	denotes	preference
T9232	29360-29363	IN	denotes	for
T9233	29364-29371	NN	denotes	thymine
T9234	29372-29376	JJ	denotes	rich
T9235	29371-29372	HYPH	denotes	-
T9236	29377-29386	NNS	denotes	sequences
T9237	29387-29393	IN	denotes	within
T9238	29394-29397	DT	denotes	the
T9239	29414-29421	NN	denotes	segment
T9240	29398-29404	JJ	denotes	single
T9241	29405-29413	VBN	denotes	stranded
T9242	29404-29405	HYPH	denotes	-
T9243	29422-29425	IN	denotes	for
T9244	29426-29434	NN	denotes	helicase
T9245	29435-29445	NN	denotes	activation
T9246	29446-29447	-LRB-	denotes	(
T9247	29447-29449	CD	denotes	13
T9248	29449-29450	-RRB-	denotes	)
T9249	29450-29451	.	denotes	.
T9250	29451-29759	sentence	denotes	The results in this report indicate that helicase action of Mcm4/6/7 on synthetic bubbles may depend on the presence of an unpaired region of sufficient length (at least 40 nt), which may permit assembly of a double-hexameric complex on the substrate DNA, similar to SV40 T-antigen protein (Figure 2A) (21).
T9251	29452-29455	DT	denotes	The
T9252	29456-29463	NNS	denotes	results
T9253	29479-29487	VBP	denotes	indicate
T9254	29464-29466	IN	denotes	in
T9255	29467-29471	DT	denotes	this
T9256	29472-29478	NN	denotes	report
T9257	29488-29492	IN	denotes	that
T9258	29546-29552	VB	denotes	depend
T9259	29493-29501	NN	denotes	helicase
T9260	29502-29508	NN	denotes	action
T9261	29509-29511	IN	denotes	of
T9262	29512-29516	NN	denotes	Mcm4
T9263	29516-29517	HYPH	denotes	/
T9264	29517-29518	CD	denotes	6
T9265	29518-29519	HYPH	denotes	/
T9266	29519-29520	CD	denotes	7
T9267	29521-29523	IN	denotes	on
T9268	29524-29533	JJ	denotes	synthetic
T9269	29534-29541	NNS	denotes	bubbles
T9270	29542-29545	MD	denotes	may
T9271	29553-29555	IN	denotes	on
T9272	29556-29559	DT	denotes	the
T9273	29560-29568	NN	denotes	presence
T9274	29569-29571	IN	denotes	of
T9275	29572-29574	DT	denotes	an
T9276	29584-29590	NN	denotes	region
T9277	29575-29583	JJ	denotes	unpaired
T9278	29591-29593	IN	denotes	of
T9279	29594-29604	JJ	denotes	sufficient
T9280	29605-29611	NN	denotes	length
T9281	29612-29613	-LRB-	denotes	(
T9282	29625-29627	NN	denotes	nt
T9283	29613-29615	RB	denotes	at
T9284	29616-29621	RBS	denotes	least
T9285	29622-29624	CD	denotes	40
T9286	29627-29628	-RRB-	denotes	)
T9287	29628-29630	,	denotes	,
T9288	29630-29635	WDT	denotes	which
T9289	29640-29646	VB	denotes	permit
T9290	29636-29639	MD	denotes	may
T9291	29647-29655	NN	denotes	assembly
T9292	29656-29658	IN	denotes	of
T9293	29659-29660	DT	denotes	a
T9294	29678-29685	NN	denotes	complex
T9295	29661-29667	JJ	denotes	double
T9296	29668-29677	JJ	denotes	hexameric
T9297	29667-29668	HYPH	denotes	-
T9298	29686-29688	IN	denotes	on
T9299	29689-29692	DT	denotes	the
T9300	29703-29706	NN	denotes	DNA
T9301	29693-29702	NN	denotes	substrate
T9302	29706-29708	,	denotes	,
T9303	29708-29715	JJ	denotes	similar
T9304	29716-29718	IN	denotes	to
T9305	29719-29723	NN	denotes	SV40
T9306	29734-29741	NN	denotes	protein
T9307	29724-29725	NN	denotes	T
T9308	29726-29733	NN	denotes	antigen
T9309	29725-29726	HYPH	denotes	-
T9310	29742-29743	-LRB-	denotes	(
T9311	29750-29752	NN	denotes	2A
T9312	29743-29749	NN	denotes	Figure
T9313	29752-29753	-RRB-	denotes	)
T9314	29754-29755	-LRB-	denotes	(
T9315	29755-29757	CD	denotes	21
T9316	29757-29758	-RRB-	denotes	)
T9317	29758-29759	.	denotes	.
T9318	29759-29918	sentence	denotes	When one strand of the single-stranded segment in T-rich bubble was replaced by guanine-rich sequences, the efficiency of unwinding was significantly reduced.
T9319	29760-29764	WRB	denotes	When
T9320	29828-29836	VBN	denotes	replaced
T9321	29765-29768	CD	denotes	one
T9322	29769-29775	NN	denotes	strand
T9323	29776-29778	IN	denotes	of
T9324	29779-29782	DT	denotes	the
T9325	29799-29806	NN	denotes	segment
T9326	29783-29789	JJ	denotes	single
T9327	29790-29798	VBN	denotes	stranded
T9328	29789-29790	HYPH	denotes	-
T9329	29807-29809	IN	denotes	in
T9330	29810-29811	NN	denotes	T
T9331	29812-29816	JJ	denotes	rich
T9332	29811-29812	HYPH	denotes	-
T9333	29817-29823	NN	denotes	bubble
T9334	29824-29827	VBD	denotes	was
T9335	29910-29917	VBN	denotes	reduced
T9336	29837-29839	IN	denotes	by
T9337	29840-29847	NN	denotes	guanine
T9338	29848-29852	JJ	denotes	rich
T9339	29847-29848	HYPH	denotes	-
T9340	29853-29862	NNS	denotes	sequences
T9341	29862-29864	,	denotes	,
T9342	29864-29867	DT	denotes	the
T9343	29868-29878	NN	denotes	efficiency
T9344	29879-29881	IN	denotes	of
T9345	29882-29891	NN	denotes	unwinding
T9346	29892-29895	VBD	denotes	was
T9347	29896-29909	RB	denotes	significantly
T9348	29917-29918	.	denotes	.
T9349	29918-30102	sentence	denotes	Our footprinting analyses showed that Mcm4/6/7 strongly protects 25 nt single-stranded DNA segment adjacent to each branch point and proximal to 5′ ends of both strands of the bubble.
T9350	29919-29922	PRP$	denotes	Our
T9351	29936-29944	NNS	denotes	analyses
T9352	29923-29935	NN	denotes	footprinting
T9353	29945-29951	VBD	denotes	showed
T9354	29952-29956	IN	denotes	that
T9355	29975-29983	VBZ	denotes	protects
T9356	29957-29961	NN	denotes	Mcm4
T9357	29961-29962	HYPH	denotes	/
T9358	29962-29963	CD	denotes	6
T9359	29963-29964	HYPH	denotes	/
T9360	29964-29965	CD	denotes	7
T9361	29966-29974	RB	denotes	strongly
T9362	29984-29986	CD	denotes	25
T9363	29987-29989	NN	denotes	nt
T9364	30010-30017	NN	denotes	segment
T9365	29990-29996	JJ	denotes	single
T9366	29997-30005	VBN	denotes	stranded
T9367	29996-29997	HYPH	denotes	-
T9368	30006-30009	NN	denotes	DNA
T9369	30018-30026	JJ	denotes	adjacent
T9370	30027-30029	IN	denotes	to
T9371	30030-30034	DT	denotes	each
T9372	30042-30047	NN	denotes	point
T9373	30035-30041	NN	denotes	branch
T9374	30048-30051	CC	denotes	and
T9375	30052-30060	JJ	denotes	proximal
T9376	30061-30063	IN	denotes	to
T9377	30064-30065	CD	denotes	5
T9378	30067-30071	NNS	denotes	ends
T9379	30065-30066	SYM	denotes	′
T9380	30072-30074	IN	denotes	of
T9381	30075-30079	DT	denotes	both
T9382	30080-30087	NNS	denotes	strands
T9383	30088-30090	IN	denotes	of
T9384	30091-30094	DT	denotes	the
T9385	30095-30101	NN	denotes	bubble
T9386	30101-30102	.	denotes	.
T9387	30102-30344	sentence	denotes	At each fork, one Mcm4/6/7 complex is likely to encircle the single-stranded DNA strand and two hexamers may bind symmetrically to the bubble substrate, forming a double hexameric structure on the bubble (see schematic drawing of Figure 1A).
T9388	30103-30105	IN	denotes	At
T9389	30138-30140	VBZ	denotes	is
T9390	30106-30110	DT	denotes	each
T9391	30111-30115	NN	denotes	fork
T9392	30115-30117	,	denotes	,
T9393	30117-30120	CD	denotes	one
T9394	30130-30137	NN	denotes	complex
T9395	30121-30125	NN	denotes	Mcm4
T9396	30125-30126	HYPH	denotes	/
T9397	30126-30127	CD	denotes	6
T9398	30127-30128	HYPH	denotes	/
T9399	30128-30129	CD	denotes	7
T9400	30141-30147	JJ	denotes	likely
T9401	30148-30150	TO	denotes	to
T9402	30151-30159	VB	denotes	encircle
T9403	30160-30163	DT	denotes	the
T9404	30184-30190	NN	denotes	strand
T9405	30164-30170	JJ	denotes	single
T9406	30171-30179	VBN	denotes	stranded
T9407	30170-30171	HYPH	denotes	-
T9408	30180-30183	NN	denotes	DNA
T9409	30191-30194	CC	denotes	and
T9410	30195-30198	CD	denotes	two
T9411	30199-30207	NNS	denotes	hexamers
T9412	30212-30216	VB	denotes	bind
T9413	30208-30211	MD	denotes	may
T9414	30217-30230	RB	denotes	symmetrically
T9415	30231-30233	IN	denotes	to
T9416	30234-30237	DT	denotes	the
T9417	30245-30254	NN	denotes	substrate
T9418	30238-30244	NN	denotes	bubble
T9419	30254-30256	,	denotes	,
T9420	30256-30263	VBG	denotes	forming
T9421	30264-30265	DT	denotes	a
T9422	30283-30292	NN	denotes	structure
T9423	30266-30272	JJ	denotes	double
T9424	30273-30282	JJ	denotes	hexameric
T9425	30293-30295	IN	denotes	on
T9426	30296-30299	DT	denotes	the
T9427	30300-30306	NN	denotes	bubble
T9428	30307-30308	-LRB-	denotes	(
T9429	30308-30311	VB	denotes	see
T9430	30312-30321	JJ	denotes	schematic
T9431	30322-30329	NN	denotes	drawing
T9432	30330-30332	IN	denotes	of
T9433	30333-30339	NN	denotes	Figure
T9434	30340-30342	NN	denotes	1A
T9435	30342-30343	-RRB-	denotes	)
T9436	30343-30344	.	denotes	.
T9437	30344-30533	sentence	denotes	Efficient unwinding into both directions may require simultaneous activation of both hexamers which may sit at the center while extruding the unwound single-stranded DNA through the rings.
T9438	30345-30354	JJ	denotes	Efficient
T9439	30355-30364	NN	denotes	unwinding
T9440	30390-30397	VB	denotes	require
T9441	30365-30369	IN	denotes	into
T9442	30370-30374	DT	denotes	both
T9443	30375-30385	NNS	denotes	directions
T9444	30386-30389	MD	denotes	may
T9445	30398-30410	JJ	denotes	simultaneous
T9446	30411-30421	NN	denotes	activation
T9447	30422-30424	IN	denotes	of
T9448	30425-30429	DT	denotes	both
T9449	30430-30438	NNS	denotes	hexamers
T9450	30439-30444	WDT	denotes	which
T9451	30449-30452	VB	denotes	sit
T9452	30445-30448	MD	denotes	may
T9453	30453-30455	IN	denotes	at
T9454	30456-30459	DT	denotes	the
T9455	30460-30466	NN	denotes	center
T9456	30467-30472	IN	denotes	while
T9457	30473-30482	VBG	denotes	extruding
T9458	30483-30486	DT	denotes	the
T9459	30511-30514	NN	denotes	DNA
T9460	30487-30494	JJ	denotes	unwound
T9461	30495-30501	JJ	denotes	single
T9462	30502-30510	VBN	denotes	stranded
T9463	30501-30502	HYPH	denotes	-
T9464	30515-30522	IN	denotes	through
T9465	30523-30526	DT	denotes	the
T9466	30527-30532	NNS	denotes	rings
T9467	30532-30533	.	denotes	.
T9468	30533-30629	sentence	denotes	This may closely resemble the proposed modes of binding and helicase actions of T-antigen (21).
T9469	30534-30538	DT	denotes	This
T9470	30551-30559	VB	denotes	resemble
T9471	30539-30542	MD	denotes	may
T9472	30543-30550	RB	denotes	closely
T9473	30560-30563	DT	denotes	the
T9474	30573-30578	NNS	denotes	modes
T9475	30564-30572	VBN	denotes	proposed
T9476	30579-30581	IN	denotes	of
T9477	30582-30589	NN	denotes	binding
T9478	30603-30610	NNS	denotes	actions
T9479	30590-30593	CC	denotes	and
T9480	30594-30602	NN	denotes	helicase
T9481	30611-30613	IN	denotes	of
T9482	30614-30615	NN	denotes	T
T9483	30616-30623	NN	denotes	antigen
T9484	30615-30616	HYPH	denotes	-
T9485	30624-30625	-LRB-	denotes	(
T9486	30625-30627	CD	denotes	21
T9487	30627-30628	-RRB-	denotes	)
T9488	30628-30629	.	denotes	.
T9489	30629-31007	sentence	denotes	Although we cannot totally exclude the possibility that one Mcm4/6/7 hexamer at each fork unwinds the duplex independently, ring-shaped structures of mouse and archea MCM, as revealed by electron microscopy, bear much similarity to the recently solved structure of the SV40 large T-antigen (4,6), and are in favor of the double-hexameric structure of mouse Mcm on a bubble DNA.
T9490	30630-30638	IN	denotes	Although
T9491	30657-30664	VB	denotes	exclude
T9492	30639-30641	PRP	denotes	we
T9493	30642-30645	MD	denotes	can
T9494	30645-30648	RB	denotes	not
T9495	30649-30656	RB	denotes	totally
T9496	30838-30842	VBP	denotes	bear
T9497	30665-30668	DT	denotes	the
T9498	30669-30680	NN	denotes	possibility
T9499	30681-30685	IN	denotes	that
T9500	30720-30727	VBZ	denotes	unwinds
T9501	30686-30689	CD	denotes	one
T9502	30699-30706	NN	denotes	hexamer
T9503	30690-30694	NN	denotes	Mcm4
T9504	30694-30695	HYPH	denotes	/
T9505	30695-30696	CD	denotes	6
T9506	30696-30697	HYPH	denotes	/
T9507	30697-30698	CD	denotes	7
T9508	30707-30709	IN	denotes	at
T9509	30710-30714	DT	denotes	each
T9510	30715-30719	NN	denotes	fork
T9511	30728-30731	DT	denotes	the
T9512	30732-30738	NN	denotes	duplex
T9513	30739-30752	RB	denotes	independently
T9514	30752-30754	,	denotes	,
T9515	30754-30758	NN	denotes	ring
T9516	30759-30765	VBN	denotes	shaped
T9517	30758-30759	HYPH	denotes	-
T9518	30766-30776	NNS	denotes	structures
T9519	30777-30779	IN	denotes	of
T9520	30780-30785	NN	denotes	mouse
T9521	30797-30800	NN	denotes	MCM
T9522	30786-30789	CC	denotes	and
T9523	30790-30796	NN	denotes	archea
T9524	30800-30802	,	denotes	,
T9525	30802-30804	IN	denotes	as
T9526	30805-30813	VBN	denotes	revealed
T9527	30814-30816	IN	denotes	by
T9528	30817-30825	NN	denotes	electron
T9529	30826-30836	NN	denotes	microscopy
T9530	30836-30838	,	denotes	,
T9531	30843-30847	JJ	denotes	much
T9532	30848-30858	NN	denotes	similarity
T9533	30859-30861	IN	denotes	to
T9534	30862-30865	DT	denotes	the
T9535	30882-30891	NN	denotes	structure
T9536	30866-30874	RB	denotes	recently
T9537	30875-30881	VBN	denotes	solved
T9538	30892-30894	IN	denotes	of
T9539	30895-30898	DT	denotes	the
T9540	30912-30919	NN	denotes	antigen
T9541	30899-30903	NN	denotes	SV40
T9542	30904-30909	JJ	denotes	large
T9543	30910-30911	NN	denotes	T
T9544	30911-30912	HYPH	denotes	-
T9545	30920-30921	-LRB-	denotes	(
T9546	30923-30924	CD	denotes	6
T9547	30921-30922	CD	denotes	4
T9548	30922-30923	,	denotes	,
T9549	30924-30925	-RRB-	denotes	)
T9550	30925-30927	,	denotes	,
T9551	30927-30930	CC	denotes	and
T9552	30931-30934	VBP	denotes	are
T9553	30935-30937	IN	denotes	in
T9554	30938-30943	NN	denotes	favor
T9555	30944-30946	IN	denotes	of
T9556	30947-30950	DT	denotes	the
T9557	30968-30977	NN	denotes	structure
T9558	30951-30957	JJ	denotes	double
T9559	30958-30967	JJ	denotes	hexameric
T9560	30957-30958	HYPH	denotes	-
T9561	30978-30980	IN	denotes	of
T9562	30981-30986	NN	denotes	mouse
T9563	30987-30990	NN	denotes	Mcm
T9564	30991-30993	IN	denotes	on
T9565	30994-30995	DT	denotes	a
T9566	31003-31006	NN	denotes	DNA
T9567	30996-31002	NN	denotes	bubble
T9568	31006-31007	.	denotes	.
T9856	31009-31018	NN	denotes	Substrate
T9857	31032-31043	NN	denotes	requirement
T9858	31019-31022	CC	denotes	and
T9859	31023-31031	NN	denotes	sequence
T9860	31044-31047	IN	denotes	for
T9861	31048-31052	NN	denotes	Mcm4
T9862	31066-31076	NN	denotes	activation
T9863	31052-31053	HYPH	denotes	/
T9864	31053-31054	CD	denotes	6
T9865	31054-31055	HYPH	denotes	/
T9866	31055-31056	CD	denotes	7
T9867	31057-31065	NN	denotes	helicase
T9868	31076-31254	sentence	denotes	DNA binding assays indicate that Mcm4/6/7 binds to those substrates containing single-stranded DNA regions regardless of the presence or absence of single-stranded 3′ or 5′ end.
T9869	31077-31080	NN	denotes	DNA
T9870	31081-31088	NN	denotes	binding
T9871	31089-31095	NNS	denotes	assays
T9872	31096-31104	VBP	denotes	indicate
T9873	31105-31109	IN	denotes	that
T9874	31119-31124	VBZ	denotes	binds
T9875	31110-31114	NN	denotes	Mcm4
T9876	31114-31115	HYPH	denotes	/
T9877	31115-31116	CD	denotes	6
T9878	31116-31117	HYPH	denotes	/
T9879	31117-31118	CD	denotes	7
T9880	31125-31127	IN	denotes	to
T9881	31128-31133	DT	denotes	those
T9882	31134-31144	NNS	denotes	substrates
T9883	31145-31155	VBG	denotes	containing
T9884	31156-31162	JJ	denotes	single
T9885	31163-31171	VBN	denotes	stranded
T9886	31162-31163	HYPH	denotes	-
T9887	31176-31183	NNS	denotes	regions
T9888	31172-31175	NN	denotes	DNA
T9889	31184-31194	RB	denotes	regardless
T9890	31195-31197	IN	denotes	of
T9891	31198-31201	DT	denotes	the
T9892	31202-31210	NN	denotes	presence
T9893	31211-31213	CC	denotes	or
T9894	31214-31221	NN	denotes	absence
T9895	31222-31224	IN	denotes	of
T9896	31225-31231	JJ	denotes	single
T9897	31232-31240	VBN	denotes	stranded
T9898	31231-31232	HYPH	denotes	-
T9899	31250-31253	NN	denotes	end
T9900	31241-31242	CD	denotes	3
T9901	31242-31243	SYM	denotes	′
T9902	31244-31246	CC	denotes	or
T9903	31247-31248	CD	denotes	5
T9904	31248-31249	SYM	denotes	′
T9905	31253-31254	.	denotes	.
T9906	31254-31355	sentence	denotes	Unwinding of the duplex DNA depends on translocation of single-stranded DNA from 3′ to 5′ direction.
T9907	31255-31264	NN	denotes	Unwinding
T9908	31283-31290	VBZ	denotes	depends
T9909	31265-31267	IN	denotes	of
T9910	31268-31271	DT	denotes	the
T9911	31279-31282	NN	denotes	DNA
T9912	31272-31278	NN	denotes	duplex
T9913	31291-31293	IN	denotes	on
T9914	31294-31307	NN	denotes	translocation
T9915	31308-31310	IN	denotes	of
T9916	31311-31317	JJ	denotes	single
T9917	31318-31326	VBN	denotes	stranded
T9918	31317-31318	HYPH	denotes	-
T9919	31327-31330	NN	denotes	DNA
T9920	31331-31335	IN	denotes	from
T9921	31336-31337	CD	denotes	3
T9922	31337-31338	SYM	denotes	′
T9923	31339-31341	IN	denotes	to
T9924	31342-31343	CD	denotes	5
T9925	31343-31344	SYM	denotes	′
T9926	31345-31354	NN	denotes	direction
T9927	31354-31355	.	denotes	.
T9928	31355-31491	sentence	denotes	This is most clearly shown by its helicase action on A-fork[5′] and 3′-extension but not on A-fork[3′], A-fork[3′,5′] nor 5′-extension.
T9929	31356-31360	DT	denotes	This
T9930	31377-31382	VBN	denotes	shown
T9931	31361-31363	VBZ	denotes	is
T9932	31364-31368	RBS	denotes	most
T9933	31369-31376	RB	denotes	clearly
T9934	31383-31385	IN	denotes	by
T9935	31386-31389	PRP$	denotes	its
T9936	31399-31405	NN	denotes	action
T9937	31390-31398	NN	denotes	helicase
T9938	31406-31408	IN	denotes	on
T9939	31409-31410	NN	denotes	A
T9940	31411-31415	NN	denotes	fork
T9941	31410-31411	HYPH	denotes	-
T9942	31427-31436	NN	denotes	extension
T9943	31415-31416	-LRB-	denotes	[
T9944	31416-31417	CD	denotes	5
T9945	31417-31418	SYM	denotes	′
T9946	31418-31419	-RRB-	denotes	]
T9947	31420-31423	CC	denotes	and
T9948	31424-31425	CD	denotes	3
T9949	31425-31426	SYM	denotes	′
T9950	31426-31427	HYPH	denotes	-
T9951	31437-31440	CC	denotes	but
T9952	31441-31444	RB	denotes	not
T9953	31445-31447	IN	denotes	on
T9954	31448-31449	NN	denotes	A
T9955	31450-31454	NN	denotes	fork
T9956	31449-31450	HYPH	denotes	-
T9957	31481-31490	NN	denotes	extension
T9958	31454-31455	-LRB-	denotes	[
T9959	31455-31456	CD	denotes	3
T9960	31456-31457	SYM	denotes	′
T9961	31457-31458	-RRB-	denotes	]
T9962	31458-31460	,	denotes	,
T9963	31460-31461	NN	denotes	A
T9964	31462-31466	NN	denotes	fork
T9965	31461-31462	HYPH	denotes	-
T9966	31466-31467	-LRB-	denotes	[
T9967	31467-31468	CD	denotes	3
T9968	31468-31469	SYM	denotes	′
T9969	31469-31470	,	denotes	,
T9970	31470-31471	CD	denotes	5
T9971	31471-31472	SYM	denotes	′
T9972	31472-31473	-RRB-	denotes	]
T9973	31474-31477	CC	denotes	nor
T9974	31478-31479	CD	denotes	5
T9975	31479-31480	SYM	denotes	′
T9976	31480-31481	HYPH	denotes	-
T9977	31490-31491	.	denotes	.
T9978	31491-31652	sentence	denotes	However, it does not necessarily require 3′ end of single-stranded DNA, since Mcm4/6/7 can displace annealing oligonucleotide on a circular single-stranded DNA.
T9979	31492-31499	RB	denotes	However
T9980	31525-31532	VB	denotes	require
T9981	31499-31501	,	denotes	,
T9982	31501-31503	PRP	denotes	it
T9983	31504-31508	VBZ	denotes	does
T9984	31509-31512	RB	denotes	not
T9985	31513-31524	RB	denotes	necessarily
T9986	31533-31534	CD	denotes	3
T9987	31536-31539	NN	denotes	end
T9988	31534-31535	SYM	denotes	′
T9989	31540-31542	IN	denotes	of
T9990	31543-31549	JJ	denotes	single
T9991	31550-31558	VBN	denotes	stranded
T9992	31549-31550	HYPH	denotes	-
T9993	31559-31562	NN	denotes	DNA
T9994	31562-31564	,	denotes	,
T9995	31564-31569	IN	denotes	since
T9996	31583-31591	VB	denotes	displace
T9997	31570-31574	NN	denotes	Mcm4
T9998	31574-31575	HYPH	denotes	/
T9999	31575-31576	CD	denotes	6
T10000	31576-31577	HYPH	denotes	/
T10001	31577-31578	CD	denotes	7
T10002	31579-31582	MD	denotes	can
T10003	31592-31601	VBG	denotes	annealing
T10004	31602-31617	NN	denotes	oligonucleotide
T10005	31618-31620	IN	denotes	on
T10006	31621-31622	DT	denotes	a
T10007	31648-31651	NN	denotes	DNA
T10008	31623-31631	JJ	denotes	circular
T10009	31632-31638	JJ	denotes	single
T10010	31639-31647	VBN	denotes	stranded
T10011	31638-31639	HYPH	denotes	-
T10012	31651-31652	.	denotes	.
T10013	31652-31810	sentence	denotes	The ability of the mouse Mcm4/6/7 to unwind 3′-extension is shared by the archaeal Mcm helicase but not by Mcm4/6/7 from S.pombe and S.cerevisiae (14,26,27).
T10014	31653-31656	DT	denotes	The
T10015	31657-31664	NN	denotes	ability
T10016	31713-31719	VBN	denotes	shared
T10017	31665-31667	IN	denotes	of
T10018	31668-31671	DT	denotes	the
T10019	31678-31682	NN	denotes	Mcm4
T10020	31672-31677	NN	denotes	mouse
T10021	31682-31683	HYPH	denotes	/
T10022	31683-31684	CD	denotes	6
T10023	31684-31685	HYPH	denotes	/
T10024	31685-31686	CD	denotes	7
T10025	31687-31689	TO	denotes	to
T10026	31690-31696	VB	denotes	unwind
T10027	31697-31698	CD	denotes	3
T10028	31700-31709	NN	denotes	extension
T10029	31698-31699	SYM	denotes	′
T10030	31699-31700	HYPH	denotes	-
T10031	31710-31712	VBZ	denotes	is
T10032	31720-31722	IN	denotes	by
T10033	31723-31726	DT	denotes	the
T10034	31740-31748	NN	denotes	helicase
T10035	31727-31735	JJ	denotes	archaeal
T10036	31736-31739	NN	denotes	Mcm
T10037	31749-31752	CC	denotes	but
T10038	31753-31756	RB	denotes	not
T10039	31757-31759	IN	denotes	by
T10040	31760-31764	NN	denotes	Mcm4
T10041	31764-31765	HYPH	denotes	/
T10042	31765-31766	CD	denotes	6
T10043	31766-31767	HYPH	denotes	/
T10044	31767-31768	CD	denotes	7
T10045	31769-31773	IN	denotes	from
T10046	31774-31781	NNP	denotes	S.pombe
T10047	31782-31785	CC	denotes	and
T10048	31786-31798	NNP	denotes	S.cerevisiae
T10049	31799-31800	-LRB-	denotes	(
T10050	31806-31808	CD	denotes	27
T10051	31800-31802	CD	denotes	14
T10052	31802-31803	,	denotes	,
T10053	31803-31805	CD	denotes	26
T10054	31805-31806	,	denotes	,
T10055	31808-31809	-RRB-	denotes	)
T10056	31809-31810	.	denotes	.
T10057	31810-31959	sentence	denotes	The archaeal Mcm can unwind A-fork[3′] but eukaryotic Mcm4/6/7 cannot, since the former binds to double-stranded DNA but the latter does not (5,27).
T10058	31811-31814	DT	denotes	The
T10059	31824-31827	NN	denotes	Mcm
T10060	31815-31823	JJ	denotes	archaeal
T10061	31832-31838	VB	denotes	unwind
T10062	31828-31831	MD	denotes	can
T10063	31839-31840	NN	denotes	A
T10064	31841-31845	NN	denotes	fork
T10065	31840-31841	HYPH	denotes	-
T10066	31845-31846	-LRB-	denotes	[
T10067	31846-31847	CD	denotes	3
T10068	31847-31848	SYM	denotes	′
T10069	31848-31849	-RRB-	denotes	]
T10070	31850-31853	CC	denotes	but
T10071	31854-31864	JJ	denotes	eukaryotic
T10072	31865-31869	NN	denotes	Mcm4
T10073	31874-31877	MD	denotes	can
T10074	31869-31870	HYPH	denotes	/
T10075	31870-31871	CD	denotes	6
T10076	31871-31872	HYPH	denotes	/
T10077	31872-31873	CD	denotes	7
T10078	31877-31880	RB	denotes	not
T10079	31880-31882	,	denotes	,
T10080	31882-31887	IN	denotes	since
T10081	31899-31904	VBZ	denotes	binds
T10082	31888-31891	DT	denotes	the
T10083	31892-31898	NN	denotes	former
T10084	31905-31907	IN	denotes	to
T10085	31908-31914	JJ	denotes	double
T10086	31915-31923	VBN	denotes	stranded
T10087	31914-31915	HYPH	denotes	-
T10088	31924-31927	NN	denotes	DNA
T10089	31928-31931	CC	denotes	but
T10090	31932-31935	DT	denotes	the
T10091	31936-31942	JJ	denotes	latter
T10092	31955-31957	CD	denotes	27
T10093	31943-31947	VBZ	denotes	does
T10094	31948-31951	RB	denotes	not
T10095	31952-31953	-LRB-	denotes	(
T10096	31953-31954	CD	denotes	5
T10097	31954-31955	,	denotes	,
T10098	31957-31958	-RRB-	denotes	)
T10099	31958-31959	.	denotes	.
T10100	31959-32089	sentence	denotes	While yeast Mcm helicases can translocate on duplex DNA, such activity was not observed with mammalian Mcm4/6/7 (data not shown).
T10101	31960-31965	IN	denotes	While
T10102	31990-32001	VB	denotes	translocate
T10103	31966-31971	NN	denotes	yeast
T10104	31972-31975	NN	denotes	Mcm
T10105	31976-31985	NNS	denotes	helicases
T10106	31986-31989	MD	denotes	can
T10107	32039-32047	VBN	denotes	observed
T10108	32002-32004	IN	denotes	on
T10109	32005-32011	NN	denotes	duplex
T10110	32012-32015	NN	denotes	DNA
T10111	32015-32017	,	denotes	,
T10112	32017-32021	JJ	denotes	such
T10113	32022-32030	NN	denotes	activity
T10114	32031-32034	VBD	denotes	was
T10115	32035-32038	RB	denotes	not
T10116	32048-32052	IN	denotes	with
T10117	32053-32062	JJ	denotes	mammalian
T10118	32063-32067	NN	denotes	Mcm4
T10119	32067-32068	HYPH	denotes	/
T10120	32068-32069	CD	denotes	6
T10121	32069-32070	HYPH	denotes	/
T10122	32070-32071	CD	denotes	7
T10123	32072-32073	-LRB-	denotes	(
T10124	32082-32087	VBN	denotes	shown
T10125	32073-32077	NNS	denotes	data
T10126	32078-32081	RB	denotes	not
T10127	32087-32088	-RRB-	denotes	)
T10128	32088-32089	.	denotes	.
T10129	32089-32426	sentence	denotes	Occurrence of AT-rich sequences, with asymmetric distribution of adenine and thymine, near the replication origins, lead us to propose that Mcm may play a role in selection of initiation sites of mammalian DNA replication, and prompted us to examine the ability of sequences from human replication origins to activate Mcm4/6/7 helicase.
T10130	32090-32100	NN	denotes	Occurrence
T10131	32206-32210	VBP	denotes	lead
T10132	32101-32103	IN	denotes	of
T10133	32104-32106	NN	denotes	AT
T10134	32107-32111	JJ	denotes	rich
T10135	32106-32107	HYPH	denotes	-
T10136	32112-32121	NNS	denotes	sequences
T10137	32121-32123	,	denotes	,
T10138	32123-32127	IN	denotes	with
T10139	32128-32138	JJ	denotes	asymmetric
T10140	32139-32151	NN	denotes	distribution
T10141	32152-32154	IN	denotes	of
T10142	32155-32162	NN	denotes	adenine
T10143	32163-32166	CC	denotes	and
T10144	32167-32174	NN	denotes	thymine
T10145	32174-32176	,	denotes	,
T10146	32176-32180	IN	denotes	near
T10147	32181-32184	DT	denotes	the
T10148	32197-32204	NNS	denotes	origins
T10149	32185-32196	NN	denotes	replication
T10150	32204-32206	,	denotes	,
T10151	32211-32213	PRP	denotes	us
T10152	32214-32216	TO	denotes	to
T10153	32217-32224	VB	denotes	propose
T10154	32225-32229	IN	denotes	that
T10155	32238-32242	VB	denotes	play
T10156	32230-32233	NNP	denotes	Mcm
T10157	32234-32237	MD	denotes	may
T10158	32243-32244	DT	denotes	a
T10159	32245-32249	NN	denotes	role
T10160	32250-32252	IN	denotes	in
T10161	32253-32262	NN	denotes	selection
T10162	32263-32265	IN	denotes	of
T10163	32266-32276	NN	denotes	initiation
T10164	32277-32282	NNS	denotes	sites
T10165	32283-32285	IN	denotes	of
T10166	32286-32295	JJ	denotes	mammalian
T10167	32296-32299	NN	denotes	DNA
T10168	32300-32311	NN	denotes	replication
T10169	32311-32313	,	denotes	,
T10170	32313-32316	CC	denotes	and
T10171	32317-32325	VBD	denotes	prompted
T10172	32326-32328	PRP	denotes	us
T10173	32329-32331	TO	denotes	to
T10174	32332-32339	VB	denotes	examine
T10175	32340-32343	DT	denotes	the
T10176	32344-32351	NN	denotes	ability
T10177	32352-32354	IN	denotes	of
T10178	32355-32364	NNS	denotes	sequences
T10179	32365-32369	IN	denotes	from
T10180	32370-32375	JJ	denotes	human
T10181	32388-32395	NNS	denotes	origins
T10182	32376-32387	NN	denotes	replication
T10183	32396-32398	TO	denotes	to
T10184	32399-32407	VB	denotes	activate
T10185	32408-32412	NN	denotes	Mcm4
T10186	32417-32425	NN	denotes	helicase
T10187	32412-32413	HYPH	denotes	/
T10188	32413-32414	CD	denotes	6
T10189	32414-32415	HYPH	denotes	/
T10190	32415-32416	CD	denotes	7
T10191	32425-32426	.	denotes	.
T10192	32426-32515	sentence	denotes	Both lamin-B2 and c-myc origins served as efficient activator for Mcm helicase in vitro.
T10193	32427-32431	CC	denotes	Both
T10194	32438-32440	NN	denotes	B2
T10195	32432-32437	NN	denotes	lamin
T10196	32437-32438	HYPH	denotes	-
T10197	32451-32458	NNS	denotes	origins
T10198	32441-32444	CC	denotes	and
T10199	32445-32446	NN	denotes	c
T10200	32447-32450	NN	denotes	myc
T10201	32446-32447	HYPH	denotes	-
T10202	32459-32465	VBD	denotes	served
T10203	32466-32468	IN	denotes	as
T10204	32469-32478	JJ	denotes	efficient
T10205	32479-32488	NN	denotes	activator
T10206	32489-32492	IN	denotes	for
T10207	32493-32496	NN	denotes	Mcm
T10208	32497-32505	NN	denotes	helicase
T10209	32506-32508	FW	denotes	in
T10210	32509-32514	FW	denotes	vitro
T10211	32514-32515	.	denotes	.
T10212	32515-32644	sentence	denotes	Consistent with it, site-specific loading of Mcm in the DNA replication initiation zone of the c-myc was recently reported (33).
T10213	32516-32526	JJ	denotes	Consistent
T10214	32630-32638	VBN	denotes	reported
T10215	32527-32531	IN	denotes	with
T10216	32532-32534	PRP	denotes	it
T10217	32534-32536	,	denotes	,
T10218	32536-32540	NN	denotes	site
T10219	32541-32549	JJ	denotes	specific
T10220	32540-32541	HYPH	denotes	-
T10221	32550-32557	NN	denotes	loading
T10222	32558-32560	IN	denotes	of
T10223	32561-32564	NN	denotes	Mcm
T10224	32565-32567	IN	denotes	in
T10225	32568-32571	DT	denotes	the
T10226	32599-32603	NN	denotes	zone
T10227	32572-32575	NN	denotes	DNA
T10228	32588-32598	NN	denotes	initiation
T10229	32576-32587	NN	denotes	replication
T10230	32604-32606	IN	denotes	of
T10231	32607-32610	DT	denotes	the
T10232	32613-32616	NN	denotes	myc
T10233	32611-32612	NN	denotes	c
T10234	32612-32613	HYPH	denotes	-
T10235	32617-32620	VBD	denotes	was
T10236	32621-32629	RB	denotes	recently
T10237	32639-32640	-LRB-	denotes	(
T10238	32640-32642	CD	denotes	33
T10239	32642-32643	-RRB-	denotes	)
T10240	32643-32644	.	denotes	.
T10241	32644-32762	sentence	denotes	We have examined in detail the effect of sequence context of the single-stranded DNA on the helicase activity of Mcm.
T10242	32645-32647	PRP	denotes	We
T10243	32653-32661	VBN	denotes	examined
T10244	32648-32652	VBP	denotes	have
T10245	32662-32664	IN	denotes	in
T10246	32665-32671	NN	denotes	detail
T10247	32672-32675	DT	denotes	the
T10248	32676-32682	NN	denotes	effect
T10249	32683-32685	IN	denotes	of
T10250	32686-32694	NN	denotes	sequence
T10251	32695-32702	NN	denotes	context
T10252	32703-32705	IN	denotes	of
T10253	32706-32709	DT	denotes	the
T10254	32726-32729	NN	denotes	DNA
T10255	32710-32716	JJ	denotes	single
T10256	32717-32725	VBN	denotes	stranded
T10257	32716-32717	HYPH	denotes	-
T10258	32730-32732	IN	denotes	on
T10259	32733-32736	DT	denotes	the
T10260	32746-32754	NN	denotes	activity
T10261	32737-32745	NN	denotes	helicase
T10262	32755-32757	IN	denotes	of
T10263	32758-32761	NN	denotes	Mcm
T10264	32761-32762	.	denotes	.
T10265	32762-32860	sentence	denotes	The results indicate that thymine content of 50% is sufficient for the maximum helicase activity.
T10266	32763-32766	DT	denotes	The
T10267	32767-32774	NNS	denotes	results
T10268	32775-32783	VBP	denotes	indicate
T10269	32784-32788	IN	denotes	that
T10270	32812-32814	VBZ	denotes	is
T10271	32789-32796	NN	denotes	thymine
T10272	32797-32804	NN	denotes	content
T10273	32805-32807	IN	denotes	of
T10274	32808-32810	CD	denotes	50
T10275	32810-32811	NN	denotes	%
T10276	32815-32825	JJ	denotes	sufficient
T10277	32826-32829	IN	denotes	for
T10278	32830-32833	DT	denotes	the
T10279	32851-32859	NN	denotes	activity
T10280	32834-32841	JJ	denotes	maximum
T10281	32842-32850	NN	denotes	helicase
T10282	32859-32860	.	denotes	.
T10283	32860-32966	sentence	denotes	The efficiency of displacement decreased as the thymine content of the 3′-tail dropped to 33% (Figure 6).
T10284	32861-32864	DT	denotes	The
T10285	32865-32875	NN	denotes	efficiency
T10286	32892-32901	VBD	denotes	decreased
T10287	32876-32878	IN	denotes	of
T10288	32879-32891	NN	denotes	displacement
T10289	32902-32904	IN	denotes	as
T10290	32940-32947	VBD	denotes	dropped
T10291	32905-32908	DT	denotes	the
T10292	32917-32924	NN	denotes	content
T10293	32909-32916	NN	denotes	thymine
T10294	32925-32927	IN	denotes	of
T10295	32928-32931	DT	denotes	the
T10296	32935-32939	NN	denotes	tail
T10297	32932-32933	CD	denotes	3
T10298	32933-32934	SYM	denotes	′
T10299	32934-32935	HYPH	denotes	-
T10300	32948-32950	IN	denotes	to
T10301	32951-32953	CD	denotes	33
T10302	32953-32954	NN	denotes	%
T10303	32955-32956	-LRB-	denotes	(
T10304	32956-32962	NN	denotes	Figure
T10305	32963-32964	CD	denotes	6
T10306	32964-32965	-RRB-	denotes	)
T10307	32965-32966	.	denotes	.
T10308	32966-33110	sentence	denotes	The stretches of thymine residues may not be necessarily required, since repeats of TC dinucleotides served as a potent activator for Mcm4/6/7.
T10309	32967-32970	DT	denotes	The
T10310	32971-32980	NNS	denotes	stretches
T10311	33024-33032	VBN	denotes	required
T10312	32981-32983	IN	denotes	of
T10313	32984-32991	NN	denotes	thymine
T10314	32992-33000	NNS	denotes	residues
T10315	33001-33004	MD	denotes	may
T10316	33005-33008	RB	denotes	not
T10317	33009-33011	VB	denotes	be
T10318	33012-33023	RB	denotes	necessarily
T10319	33032-33034	,	denotes	,
T10320	33034-33039	IN	denotes	since
T10321	33068-33074	VBD	denotes	served
T10322	33040-33047	NNS	denotes	repeats
T10323	33048-33050	IN	denotes	of
T10324	33051-33053	NN	denotes	TC
T10325	33054-33067	NNS	denotes	dinucleotides
T10326	33075-33077	IN	denotes	as
T10327	33078-33079	DT	denotes	a
T10328	33087-33096	NN	denotes	activator
T10329	33080-33086	JJ	denotes	potent
T10330	33097-33100	IN	denotes	for
T10331	33101-33105	NN	denotes	Mcm4
T10332	33105-33106	HYPH	denotes	/
T10333	33106-33107	CD	denotes	6
T10334	33107-33108	HYPH	denotes	/
T10335	33108-33109	CD	denotes	7
T10336	33109-33110	.	denotes	.
T10337	33110-33235	sentence	denotes	We also noticed that the presence of a secondary structure within the single-stranded DNA is inhibitory for helicase action.
T10338	33111-33113	PRP	denotes	We
T10339	33119-33126	VBD	denotes	noticed
T10340	33114-33118	RB	denotes	also
T10341	33127-33131	IN	denotes	that
T10342	33201-33203	VBZ	denotes	is
T10343	33132-33135	DT	denotes	the
T10344	33136-33144	NN	denotes	presence
T10345	33145-33147	IN	denotes	of
T10346	33148-33149	DT	denotes	a
T10347	33160-33169	NN	denotes	structure
T10348	33150-33159	JJ	denotes	secondary
T10349	33170-33176	IN	denotes	within
T10350	33177-33180	DT	denotes	the
T10351	33197-33200	NN	denotes	DNA
T10352	33181-33187	JJ	denotes	single
T10353	33188-33196	VBN	denotes	stranded
T10354	33187-33188	HYPH	denotes	-
T10355	33204-33214	JJ	denotes	inhibitory
T10356	33215-33218	IN	denotes	for
T10357	33219-33227	NN	denotes	helicase
T10358	33228-33234	NN	denotes	action
T10359	33234-33235	.	denotes	.
T10360	33235-33347	sentence	denotes	Nuclease footprinting assays indicated that binding was interfered by the secondary structure (data not shown).
T10361	33236-33244	NN	denotes	Nuclease
T10362	33245-33257	NN	denotes	footprinting
T10363	33258-33264	NNS	denotes	assays
T10364	33265-33274	VBD	denotes	indicated
T10365	33275-33279	IN	denotes	that
T10366	33292-33302	VBN	denotes	interfered
T10367	33280-33287	NN	denotes	binding
T10368	33288-33291	VBD	denotes	was
T10369	33303-33305	IN	denotes	by
T10370	33306-33309	DT	denotes	the
T10371	33320-33329	NN	denotes	structure
T10372	33310-33319	JJ	denotes	secondary
T10373	33330-33331	-LRB-	denotes	(
T10374	33340-33345	VBN	denotes	shown
T10375	33331-33335	NNS	denotes	data
T10376	33336-33339	RB	denotes	not
T10377	33345-33346	-RRB-	denotes	)
T10378	33346-33347	.	denotes	.
T10379	33347-33755	sentence	denotes	Thus, we have concluded that Mcm4/6/7 helicase is most efficiently activated by non-structured single-stranded DNA with thymine content of 50% or more, although significant stimulation is observed also by DNA with less thymine content (Figure 7), suggesting that the sequence specificity for Mcm helicase activation is rather relaxed and that the extent of the activation may depend on the sequence context.
T10380	33348-33352	RB	denotes	Thus
T10381	33362-33371	VBN	denotes	concluded
T10382	33352-33354	,	denotes	,
T10383	33354-33356	PRP	denotes	we
T10384	33357-33361	VBP	denotes	have
T10385	33372-33376	IN	denotes	that
T10386	33415-33424	VBN	denotes	activated
T10387	33377-33381	NN	denotes	Mcm4
T10388	33386-33394	NN	denotes	helicase
T10389	33381-33382	HYPH	denotes	/
T10390	33382-33383	CD	denotes	6
T10391	33383-33384	HYPH	denotes	/
T10392	33384-33385	CD	denotes	7
T10393	33395-33397	VBZ	denotes	is
T10394	33398-33402	RBS	denotes	most
T10395	33403-33414	RB	denotes	efficiently
T10396	33425-33427	IN	denotes	by
T10397	33428-33442	JJ	denotes	non-structured
T10398	33459-33462	NN	denotes	DNA
T10399	33443-33449	JJ	denotes	single
T10400	33450-33458	VBN	denotes	stranded
T10401	33449-33450	HYPH	denotes	-
T10402	33463-33467	IN	denotes	with
T10403	33468-33475	NN	denotes	thymine
T10404	33476-33483	NN	denotes	content
T10405	33484-33486	IN	denotes	of
T10406	33487-33489	CD	denotes	50
T10407	33489-33490	NN	denotes	%
T10408	33491-33493	CC	denotes	or
T10409	33494-33498	JJR	denotes	more
T10410	33498-33500	,	denotes	,
T10411	33500-33508	IN	denotes	although
T10412	33536-33544	VBN	denotes	observed
T10413	33509-33520	JJ	denotes	significant
T10414	33521-33532	NN	denotes	stimulation
T10415	33533-33535	VBZ	denotes	is
T10416	33545-33549	RB	denotes	also
T10417	33550-33552	IN	denotes	by
T10418	33553-33556	NN	denotes	DNA
T10419	33557-33561	IN	denotes	with
T10420	33562-33566	JJR	denotes	less
T10421	33575-33582	NN	denotes	content
T10422	33567-33574	NN	denotes	thymine
T10423	33583-33584	-LRB-	denotes	(
T10424	33584-33590	NN	denotes	Figure
T10425	33591-33592	CD	denotes	7
T10426	33592-33593	-RRB-	denotes	)
T10427	33593-33595	,	denotes	,
T10428	33595-33605	VBG	denotes	suggesting
T10429	33606-33610	IN	denotes	that
T10430	33664-33666	VBZ	denotes	is
T10431	33611-33614	DT	denotes	the
T10432	33624-33635	NN	denotes	specificity
T10433	33615-33623	NN	denotes	sequence
T10434	33636-33639	IN	denotes	for
T10435	33640-33643	NN	denotes	Mcm
T10436	33644-33652	NN	denotes	helicase
T10437	33653-33663	NN	denotes	activation
T10438	33667-33673	RB	denotes	rather
T10439	33674-33681	JJ	denotes	relaxed
T10440	33682-33685	CC	denotes	and
T10441	33686-33690	IN	denotes	that
T10442	33724-33730	VB	denotes	depend
T10443	33691-33694	DT	denotes	the
T10444	33695-33701	NN	denotes	extent
T10445	33702-33704	IN	denotes	of
T10446	33705-33708	DT	denotes	the
T10447	33709-33719	NN	denotes	activation
T10448	33720-33723	MD	denotes	may
T10449	33731-33733	IN	denotes	on
T10450	33734-33737	DT	denotes	the
T10451	33747-33754	NN	denotes	context
T10452	33738-33746	NN	denotes	sequence
T10453	33754-33755	.	denotes	.
T10454	33755-34053	sentence	denotes	This would be reasonable given the flexibility and differential regulation of site selection for initiation of DNA replication during development or in various cell types, as well as the variability in initiation potential of each replication origin on the genome even within the single cell type.
T10455	33756-33760	DT	denotes	This
T10456	33767-33769	VB	denotes	be
T10457	33761-33766	MD	denotes	would
T10458	33770-33780	JJ	denotes	reasonable
T10459	33781-33786	VBN	denotes	given
T10460	33787-33790	DT	denotes	the
T10461	33791-33802	NN	denotes	flexibility
T10462	33803-33806	CC	denotes	and
T10463	33807-33819	JJ	denotes	differential
T10464	33820-33830	NN	denotes	regulation
T10465	33831-33833	IN	denotes	of
T10466	33834-33838	NN	denotes	site
T10467	33839-33848	NN	denotes	selection
T10468	33849-33852	IN	denotes	for
T10469	33853-33863	NN	denotes	initiation
T10470	33864-33866	IN	denotes	of
T10471	33867-33870	NN	denotes	DNA
T10472	33871-33882	NN	denotes	replication
T10473	33883-33889	IN	denotes	during
T10474	33890-33901	NN	denotes	development
T10475	33902-33904	CC	denotes	or
T10476	33905-33907	IN	denotes	in
T10477	33908-33915	JJ	denotes	various
T10478	33921-33926	NNS	denotes	types
T10479	33916-33920	NN	denotes	cell
T10480	33926-33928	,	denotes	,
T10481	33928-33930	RB	denotes	as
T10482	33936-33938	IN	denotes	as
T10483	33931-33935	RB	denotes	well
T10484	33939-33942	DT	denotes	the
T10485	33943-33954	NN	denotes	variability
T10486	33955-33957	IN	denotes	in
T10487	33958-33968	NN	denotes	initiation
T10488	33969-33978	NN	denotes	potential
T10489	33979-33981	IN	denotes	of
T10490	33982-33986	DT	denotes	each
T10491	33999-34005	NN	denotes	origin
T10492	33987-33998	NN	denotes	replication
T10493	34006-34008	IN	denotes	on
T10494	34009-34012	DT	denotes	the
T10495	34013-34019	NN	denotes	genome
T10496	34020-34024	RB	denotes	even
T10497	34025-34031	IN	denotes	within
T10498	34032-34035	DT	denotes	the
T10499	34048-34052	NN	denotes	type
T10500	34036-34042	JJ	denotes	single
T10501	34043-34047	NN	denotes	cell
T10502	34052-34053	.	denotes	.
T10855	34055-34059	NN	denotes	Mcm4
T10856	34064-34072	NN	denotes	helicase
T10857	34059-34060	HYPH	denotes	/
T10858	34060-34061	CD	denotes	6
T10859	34061-34062	HYPH	denotes	/
T10860	34062-34063	CD	denotes	7
T10861	34073-34079	IN	denotes	during
T10862	34080-34090	JJ	denotes	processive
T10863	34091-34100	NN	denotes	unwinding
T10864	34101-34103	IN	denotes	at
T10865	34104-34107	DT	denotes	the
T10866	34108-34112	NN	denotes	fork
T10867	34112-34298	sentence	denotes	The specific requirement of single-stranded thymine residues for activation of Mcm helicase prompted us to examine whether they are required also for processive unwinding of duplex DNA.
T10868	34113-34116	DT	denotes	The
T10869	34126-34137	NN	denotes	requirement
T10870	34117-34125	JJ	denotes	specific
T10871	34205-34213	VBD	denotes	prompted
T10872	34138-34140	IN	denotes	of
T10873	34141-34147	JJ	denotes	single
T10874	34148-34156	VBN	denotes	stranded
T10875	34147-34148	HYPH	denotes	-
T10876	34165-34173	NNS	denotes	residues
T10877	34157-34164	NN	denotes	thymine
T10878	34174-34177	IN	denotes	for
T10879	34178-34188	NN	denotes	activation
T10880	34189-34191	IN	denotes	of
T10881	34192-34195	NN	denotes	Mcm
T10882	34196-34204	NN	denotes	helicase
T10883	34214-34216	PRP	denotes	us
T10884	34217-34219	TO	denotes	to
T10885	34220-34227	VB	denotes	examine
T10886	34228-34235	IN	denotes	whether
T10887	34245-34253	VBN	denotes	required
T10888	34236-34240	PRP	denotes	they
T10889	34241-34244	VBP	denotes	are
T10890	34254-34258	RB	denotes	also
T10891	34259-34262	IN	denotes	for
T10892	34263-34273	JJ	denotes	processive
T10893	34274-34283	NN	denotes	unwinding
T10894	34284-34286	IN	denotes	of
T10895	34287-34293	NN	denotes	duplex
T10896	34294-34297	NN	denotes	DNA
T10897	34297-34298	.	denotes	.
T10898	34298-34418	sentence	denotes	Our results indicated that increase of GC pair in the duplex segment significantly inhibited the Mcm helicase activity.
T10899	34299-34302	PRP$	denotes	Our
T10900	34303-34310	NNS	denotes	results
T10901	34311-34320	VBD	denotes	indicated
T10902	34321-34325	IN	denotes	that
T10903	34382-34391	VBD	denotes	inhibited
T10904	34326-34334	NN	denotes	increase
T10905	34335-34337	IN	denotes	of
T10906	34338-34340	NN	denotes	GC
T10907	34341-34345	NN	denotes	pair
T10908	34346-34348	IN	denotes	in
T10909	34349-34352	DT	denotes	the
T10910	34360-34367	NN	denotes	segment
T10911	34353-34359	NN	denotes	duplex
T10912	34368-34381	RB	denotes	significantly
T10913	34392-34395	DT	denotes	the
T10914	34409-34417	NN	denotes	activity
T10915	34396-34399	NN	denotes	Mcm
T10916	34400-34408	NN	denotes	helicase
T10917	34417-34418	.	denotes	.
T10918	34418-34590	sentence	denotes	Duplex DNA composed only of GC pairs (10 repeats of CGG) on a Y-fork was not displaced at all, while the same template was readily displaced by SV40 T-antigen (Figure 8B).
T10919	34419-34425	NN	denotes	Duplex
T10920	34426-34429	NN	denotes	DNA
T10921	34496-34505	VBN	denotes	displaced
T10922	34430-34438	VBN	denotes	composed
T10923	34439-34443	RB	denotes	only
T10924	34444-34446	IN	denotes	of
T10925	34447-34449	NN	denotes	GC
T10926	34450-34455	NNS	denotes	pairs
T10927	34456-34457	-LRB-	denotes	(
T10928	34460-34467	NNS	denotes	repeats
T10929	34457-34459	CD	denotes	10
T10930	34468-34470	IN	denotes	of
T10931	34471-34474	NN	denotes	CGG
T10932	34474-34475	-RRB-	denotes	)
T10933	34476-34478	IN	denotes	on
T10934	34479-34480	DT	denotes	a
T10935	34483-34487	NN	denotes	fork
T10936	34481-34482	NN	denotes	Y
T10937	34482-34483	HYPH	denotes	-
T10938	34488-34491	VBD	denotes	was
T10939	34492-34495	RB	denotes	not
T10940	34506-34508	RB	denotes	at
T10941	34509-34512	RB	denotes	all
T10942	34512-34514	,	denotes	,
T10943	34514-34519	IN	denotes	while
T10944	34550-34559	VBN	denotes	displaced
T10945	34520-34523	DT	denotes	the
T10946	34529-34537	NN	denotes	template
T10947	34524-34528	JJ	denotes	same
T10948	34538-34541	VBD	denotes	was
T10949	34542-34549	RB	denotes	readily
T10950	34560-34562	IN	denotes	by
T10951	34563-34567	NN	denotes	SV40
T10952	34570-34577	NN	denotes	antigen
T10953	34568-34569	NN	denotes	T
T10954	34569-34570	HYPH	denotes	-
T10955	34578-34579	-LRB-	denotes	(
T10956	34586-34588	NN	denotes	8B
T10957	34579-34585	NN	denotes	Figure
T10958	34588-34589	-RRB-	denotes	)
T10959	34589-34590	.	denotes	.
T10960	34590-34726	sentence	denotes	Mcm helicase was inhibited by the presence of GC-rich duplex segment also on a circular single-stranded partial heteroduplex substrate.
T10961	34591-34594	NN	denotes	Mcm
T10962	34595-34603	NN	denotes	helicase
T10963	34608-34617	VBN	denotes	inhibited
T10964	34604-34607	VBD	denotes	was
T10965	34618-34620	IN	denotes	by
T10966	34621-34624	DT	denotes	the
T10967	34625-34633	NN	denotes	presence
T10968	34634-34636	IN	denotes	of
T10969	34637-34639	NN	denotes	GC
T10970	34640-34644	JJ	denotes	rich
T10971	34639-34640	HYPH	denotes	-
T10972	34652-34659	NN	denotes	segment
T10973	34645-34651	NN	denotes	duplex
T10974	34660-34664	RB	denotes	also
T10975	34665-34667	IN	denotes	on
T10976	34668-34669	DT	denotes	a
T10977	34716-34725	NN	denotes	substrate
T10978	34670-34678	JJ	denotes	circular
T10979	34679-34685	JJ	denotes	single
T10980	34686-34694	VBN	denotes	stranded
T10981	34685-34686	HYPH	denotes	-
T10982	34695-34702	JJ	denotes	partial
T10983	34703-34715	NN	denotes	heteroduplex
T10984	34725-34726	.	denotes	.
T10985	34726-34884	sentence	denotes	However, on this substrate, Mcm4/6/7 was able to displace DNA past the GC-rich region, albeit to a limited extent, when it was added at a high concentration.
T10986	34727-34734	RB	denotes	However
T10987	34764-34767	VBD	denotes	was
T10988	34734-34736	,	denotes	,
T10989	34736-34738	IN	denotes	on
T10990	34739-34743	DT	denotes	this
T10991	34744-34753	NN	denotes	substrate
T10992	34753-34755	,	denotes	,
T10993	34755-34759	NN	denotes	Mcm4
T10994	34759-34760	HYPH	denotes	/
T10995	34760-34761	CD	denotes	6
T10996	34761-34762	HYPH	denotes	/
T10997	34762-34763	CD	denotes	7
T10998	34768-34772	JJ	denotes	able
T10999	34773-34775	TO	denotes	to
T11000	34776-34784	VB	denotes	displace
T11001	34785-34788	NN	denotes	DNA
T11002	34789-34793	IN	denotes	past
T11003	34794-34797	DT	denotes	the
T11004	34806-34812	NN	denotes	region
T11005	34798-34800	NN	denotes	GC
T11006	34801-34805	JJ	denotes	rich
T11007	34800-34801	HYPH	denotes	-
T11008	34812-34814	,	denotes	,
T11009	34814-34820	IN	denotes	albeit
T11010	34821-34823	IN	denotes	to
T11011	34824-34825	DT	denotes	a
T11012	34834-34840	NN	denotes	extent
T11013	34826-34833	VBN	denotes	limited
T11014	34840-34842	,	denotes	,
T11015	34842-34846	WRB	denotes	when
T11016	34854-34859	VBN	denotes	added
T11017	34847-34849	PRP	denotes	it
T11018	34850-34853	VBD	denotes	was
T11019	34860-34862	IN	denotes	at
T11020	34863-34864	DT	denotes	a
T11021	34870-34883	NN	denotes	concentration
T11022	34865-34869	JJ	denotes	high
T11023	34883-34884	.	denotes	.
T11024	34884-35051	sentence	denotes	On the partial heteroduplex template, Mcm is loaded onto the circular single-stranded DNA of 6.4 kb, while it is loaded onto the 50 nt long 3′-tail DNA on the Y-fork.
T11025	34885-34887	IN	denotes	On
T11026	34930-34936	VBN	denotes	loaded
T11027	34888-34891	DT	denotes	the
T11028	34913-34921	NN	denotes	template
T11029	34892-34899	JJ	denotes	partial
T11030	34900-34912	NN	denotes	heteroduplex
T11031	34921-34923	,	denotes	,
T11032	34923-34926	NN	denotes	Mcm
T11033	34927-34929	VBZ	denotes	is
T11034	34937-34941	IN	denotes	onto
T11035	34942-34945	DT	denotes	the
T11036	34971-34974	NN	denotes	DNA
T11037	34946-34954	JJ	denotes	circular
T11038	34955-34961	JJ	denotes	single
T11039	34962-34970	VBN	denotes	stranded
T11040	34961-34962	HYPH	denotes	-
T11041	34975-34977	IN	denotes	of
T11042	34978-34981	CD	denotes	6.4
T11043	34982-34984	NN	denotes	kb
T11044	34984-34986	,	denotes	,
T11045	34986-34991	IN	denotes	while
T11046	34998-35004	VBN	denotes	loaded
T11047	34992-34994	PRP	denotes	it
T11048	34995-34997	VBZ	denotes	is
T11049	35005-35009	IN	denotes	onto
T11050	35010-35013	DT	denotes	the
T11051	35033-35036	NN	denotes	DNA
T11052	35014-35016	CD	denotes	50
T11053	35017-35019	NN	denotes	nt
T11054	35020-35024	JJ	denotes	long
T11055	35025-35026	CD	denotes	3
T11056	35028-35032	NN	denotes	tail
T11057	35026-35027	SYM	denotes	′
T11058	35027-35028	HYPH	denotes	-
T11059	35037-35039	IN	denotes	on
T11060	35040-35043	DT	denotes	the
T11061	35046-35050	NN	denotes	fork
T11062	35044-35045	NN	denotes	Y
T11063	35045-35046	HYPH	denotes	-
T11064	35050-35051	.	denotes	.
T11065	35051-35135	sentence	denotes	Thus, the difference of helicase actions may reflect the efficiency of Mcm loading.
T11066	35052-35056	RB	denotes	Thus
T11067	35097-35104	VB	denotes	reflect
T11068	35056-35058	,	denotes	,
T11069	35058-35061	DT	denotes	the
T11070	35062-35072	NN	denotes	difference
T11071	35073-35075	IN	denotes	of
T11072	35076-35084	NN	denotes	helicase
T11073	35085-35092	NNS	denotes	actions
T11074	35093-35096	MD	denotes	may
T11075	35105-35108	DT	denotes	the
T11076	35109-35119	NN	denotes	efficiency
T11077	35120-35122	IN	denotes	of
T11078	35123-35126	NN	denotes	Mcm
T11079	35127-35134	NN	denotes	loading
T11080	35134-35135	.	denotes	.
T11081	35135-35352	sentence	denotes	Alternatively, the presence of ‘random’ sequence at the initially unwound duplex segment in the former template may engage the Mcm helicase in a more active conformation which can displace the GC-rich duplex segment.
T11082	35136-35149	RB	denotes	Alternatively
T11083	35252-35258	VB	denotes	engage
T11084	35149-35151	,	denotes	,
T11085	35151-35154	DT	denotes	the
T11086	35155-35163	NN	denotes	presence
T11087	35164-35166	IN	denotes	of
T11088	35167-35168	``	denotes	‘
T11089	35168-35174	JJ	denotes	random
T11090	35176-35184	NN	denotes	sequence
T11091	35174-35175	''	denotes	’
T11092	35185-35187	IN	denotes	at
T11093	35188-35191	DT	denotes	the
T11094	35217-35224	NN	denotes	segment
T11095	35192-35201	RB	denotes	initially
T11096	35202-35209	JJ	denotes	unwound
T11097	35210-35216	NN	denotes	duplex
T11098	35225-35227	IN	denotes	in
T11099	35228-35231	DT	denotes	the
T11100	35239-35247	NN	denotes	template
T11101	35232-35238	JJ	denotes	former
T11102	35248-35251	MD	denotes	may
T11103	35259-35262	DT	denotes	the
T11104	35267-35275	NN	denotes	helicase
T11105	35263-35266	NN	denotes	Mcm
T11106	35276-35278	IN	denotes	in
T11107	35279-35280	DT	denotes	a
T11108	35293-35305	NN	denotes	conformation
T11109	35281-35285	RBR	denotes	more
T11110	35286-35292	JJ	denotes	active
T11111	35306-35311	WDT	denotes	which
T11112	35316-35324	VB	denotes	displace
T11113	35312-35315	MD	denotes	can
T11114	35325-35328	DT	denotes	the
T11115	35344-35351	NN	denotes	segment
T11116	35329-35331	NN	denotes	GC
T11117	35332-35336	JJ	denotes	rich
T11118	35331-35332	HYPH	denotes	-
T11119	35337-35343	NN	denotes	duplex
T11120	35351-35352	.	denotes	.
T11121	35352-35458	sentence	denotes	On Y-fork substrates, increase of AT base pairs in the duplex (10 repeats of CTT) restored the unwinding.
T11122	35353-35355	IN	denotes	On
T11123	35435-35443	VBD	denotes	restored
T11124	35356-35357	NN	denotes	Y
T11125	35358-35362	NN	denotes	fork
T11126	35357-35358	HYPH	denotes	-
T11127	35363-35373	NNS	denotes	substrates
T11128	35373-35375	,	denotes	,
T11129	35375-35383	NN	denotes	increase
T11130	35384-35386	IN	denotes	of
T11131	35387-35389	NN	denotes	AT
T11132	35395-35400	NNS	denotes	pairs
T11133	35390-35394	NN	denotes	base
T11134	35401-35403	IN	denotes	in
T11135	35404-35407	DT	denotes	the
T11136	35408-35414	NN	denotes	duplex
T11137	35415-35416	-LRB-	denotes	(
T11138	35419-35426	NNS	denotes	repeats
T11139	35416-35418	CD	denotes	10
T11140	35427-35429	IN	denotes	of
T11141	35430-35433	NN	denotes	CTT
T11142	35433-35434	-RRB-	denotes	)
T11143	35444-35447	DT	denotes	the
T11144	35448-35457	NN	denotes	unwinding
T11145	35457-35458	.	denotes	.
T11146	35458-35646	sentence	denotes	These results indicate either that thymine residues are required on the duplex DNA for continuous unwinding, or that Mcm4/6/7 is simply not efficient enough to unwind the stable GC pairs.
T11147	35459-35464	DT	denotes	These
T11148	35465-35472	NNS	denotes	results
T11149	35473-35481	VBP	denotes	indicate
T11150	35482-35488	CC	denotes	either
T11151	35515-35523	VBN	denotes	required
T11152	35489-35493	IN	denotes	that
T11153	35494-35501	NN	denotes	thymine
T11154	35502-35510	NNS	denotes	residues
T11155	35511-35514	VBP	denotes	are
T11156	35524-35526	IN	denotes	on
T11157	35527-35530	DT	denotes	the
T11158	35538-35541	NN	denotes	DNA
T11159	35531-35537	NN	denotes	duplex
T11160	35542-35545	IN	denotes	for
T11161	35546-35556	JJ	denotes	continuous
T11162	35557-35566	NN	denotes	unwinding
T11163	35566-35568	,	denotes	,
T11164	35568-35570	CC	denotes	or
T11165	35571-35575	IN	denotes	that
T11166	35585-35587	VBZ	denotes	is
T11167	35576-35580	NN	denotes	Mcm4
T11168	35580-35581	HYPH	denotes	/
T11169	35581-35582	CD	denotes	6
T11170	35582-35583	HYPH	denotes	/
T11171	35583-35584	CD	denotes	7
T11172	35588-35594	RB	denotes	simply
T11173	35595-35598	RB	denotes	not
T11174	35599-35608	JJ	denotes	efficient
T11175	35609-35615	RB	denotes	enough
T11176	35616-35618	TO	denotes	to
T11177	35619-35625	VB	denotes	unwind
T11178	35626-35629	DT	denotes	the
T11179	35640-35645	NNS	denotes	pairs
T11180	35630-35636	JJ	denotes	stable
T11181	35637-35639	NN	denotes	GC
T11182	35645-35646	.	denotes	.
T11183	35646-35968	sentence	denotes	Replacement of the central guanosine with inosine in the CGG repeat duplex DNA resulted in displacement (Figure 8E) (31), suggesting that the continuous presence of AT base pair may not be essential for the Mcm helicase function and that the thermostability of GC base pairs is inhibitory for helicase action of Mcm4/6/7.
T11184	35647-35658	NN	denotes	Replacement
T11185	35726-35734	VBD	denotes	resulted
T11186	35659-35661	IN	denotes	of
T11187	35662-35665	DT	denotes	the
T11188	35674-35683	NN	denotes	guanosine
T11189	35666-35673	JJ	denotes	central
T11190	35684-35688	IN	denotes	with
T11191	35689-35696	NN	denotes	inosine
T11192	35697-35699	IN	denotes	in
T11193	35700-35703	DT	denotes	the
T11194	35722-35725	NN	denotes	DNA
T11195	35704-35707	NN	denotes	CGG
T11196	35708-35714	NN	denotes	repeat
T11197	35715-35721	NN	denotes	duplex
T11198	35735-35737	IN	denotes	in
T11199	35738-35750	NN	denotes	displacement
T11200	35751-35752	-LRB-	denotes	(
T11201	35759-35761	NN	denotes	8E
T11202	35752-35758	NN	denotes	Figure
T11203	35761-35762	-RRB-	denotes	)
T11204	35763-35764	-LRB-	denotes	(
T11205	35764-35766	CD	denotes	31
T11206	35766-35767	-RRB-	denotes	)
T11207	35767-35769	,	denotes	,
T11208	35769-35779	VBG	denotes	suggesting
T11209	35780-35784	IN	denotes	that
T11210	35833-35835	VB	denotes	be
T11211	35785-35788	DT	denotes	the
T11212	35800-35808	NN	denotes	presence
T11213	35789-35799	JJ	denotes	continuous
T11214	35809-35811	IN	denotes	of
T11215	35812-35814	NN	denotes	AT
T11216	35820-35824	NN	denotes	pair
T11217	35815-35819	NN	denotes	base
T11218	35825-35828	MD	denotes	may
T11219	35829-35832	RB	denotes	not
T11220	35836-35845	JJ	denotes	essential
T11221	35846-35849	IN	denotes	for
T11222	35850-35853	DT	denotes	the
T11223	35867-35875	NN	denotes	function
T11224	35854-35857	NN	denotes	Mcm
T11225	35858-35866	NN	denotes	helicase
T11226	35876-35879	CC	denotes	and
T11227	35880-35884	IN	denotes	that
T11228	35922-35924	VBZ	denotes	is
T11229	35885-35888	DT	denotes	the
T11230	35889-35904	NN	denotes	thermostability
T11231	35905-35907	IN	denotes	of
T11232	35908-35910	NN	denotes	GC
T11233	35916-35921	NNS	denotes	pairs
T11234	35911-35915	NN	denotes	base
T11235	35925-35935	JJ	denotes	inhibitory
T11236	35936-35939	IN	denotes	for
T11237	35940-35948	NN	denotes	helicase
T11238	35949-35955	NN	denotes	action
T11239	35956-35958	IN	denotes	of
T11240	35959-35963	NN	denotes	Mcm4
T11241	35963-35964	HYPH	denotes	/
T11242	35964-35965	CD	denotes	6
T11243	35965-35966	HYPH	denotes	/
T11244	35966-35967	CD	denotes	7
T11245	35967-35968	.	denotes	.
T11246	35968-36157	sentence	denotes	The results described in this manuscript reveal potentially important features of mammalian Mcm4/6/7 helicase, which is likely to be a key component of the eukaryotic replicative helicase.
T11247	35969-35972	DT	denotes	The
T11248	35973-35980	NNS	denotes	results
T11249	36010-36016	VBP	denotes	reveal
T11250	35981-35990	VBN	denotes	described
T11251	35991-35993	IN	denotes	in
T11252	35994-35998	DT	denotes	this
T11253	35999-36009	NN	denotes	manuscript
T11254	36017-36028	RB	denotes	potentially
T11255	36029-36038	JJ	denotes	important
T11256	36039-36047	NNS	denotes	features
T11257	36048-36050	IN	denotes	of
T11258	36051-36060	JJ	denotes	mammalian
T11259	36070-36078	NN	denotes	helicase
T11260	36061-36065	NN	denotes	Mcm4
T11261	36065-36066	HYPH	denotes	/
T11262	36066-36067	CD	denotes	6
T11263	36067-36068	HYPH	denotes	/
T11264	36068-36069	CD	denotes	7
T11265	36078-36080	,	denotes	,
T11266	36080-36085	WDT	denotes	which
T11267	36086-36088	VBZ	denotes	is
T11268	36089-36095	JJ	denotes	likely
T11269	36096-36098	TO	denotes	to
T11270	36099-36101	VB	denotes	be
T11271	36102-36103	DT	denotes	a
T11272	36108-36117	NN	denotes	component
T11273	36104-36107	JJ	denotes	key
T11274	36118-36120	IN	denotes	of
T11275	36121-36124	DT	denotes	the
T11276	36148-36156	NN	denotes	helicase
T11277	36125-36135	JJ	denotes	eukaryotic
T11278	36136-36147	JJ	denotes	replicative
T11279	36156-36157	.	denotes	.
T11280	36157-36421	sentence	denotes	Prior to initiation of DNA replication, Mcm helicase may adopt a double hexameric complex at the partially melted origin region, and may catalyze concurrent unwinding of duplex DNA into both directions, while stably associated with the origins of DNA replication.
T11281	36158-36163	JJ	denotes	Prior
T11282	36164-36166	IN	denotes	to
T11283	36215-36220	VB	denotes	adopt
T11284	36167-36177	NN	denotes	initiation
T11285	36178-36180	IN	denotes	of
T11286	36181-36184	NN	denotes	DNA
T11287	36185-36196	NN	denotes	replication
T11288	36196-36198	,	denotes	,
T11289	36198-36201	NN	denotes	Mcm
T11290	36202-36210	NN	denotes	helicase
T11291	36211-36214	MD	denotes	may
T11292	36221-36222	DT	denotes	a
T11293	36240-36247	NN	denotes	complex
T11294	36223-36229	JJ	denotes	double
T11295	36230-36239	JJ	denotes	hexameric
T11296	36248-36250	IN	denotes	at
T11297	36251-36254	DT	denotes	the
T11298	36279-36285	NN	denotes	region
T11299	36255-36264	RB	denotes	partially
T11300	36265-36271	VBN	denotes	melted
T11301	36272-36278	NN	denotes	origin
T11302	36285-36287	,	denotes	,
T11303	36287-36290	CC	denotes	and
T11304	36291-36294	MD	denotes	may
T11305	36295-36303	VB	denotes	catalyze
T11306	36304-36314	JJ	denotes	concurrent
T11307	36315-36324	NN	denotes	unwinding
T11308	36325-36327	IN	denotes	of
T11309	36328-36334	NN	denotes	duplex
T11310	36335-36338	NN	denotes	DNA
T11311	36339-36343	IN	denotes	into
T11312	36344-36348	DT	denotes	both
T11313	36349-36359	NNS	denotes	directions
T11314	36359-36361	,	denotes	,
T11315	36361-36366	IN	denotes	while
T11316	36374-36384	VBN	denotes	associated
T11317	36367-36373	RB	denotes	stably
T11318	36385-36389	IN	denotes	with
T11319	36390-36393	DT	denotes	the
T11320	36394-36401	NNS	denotes	origins
T11321	36402-36404	IN	denotes	of
T11322	36405-36408	NN	denotes	DNA
T11323	36409-36420	NN	denotes	replication
T11324	36420-36421	.	denotes	.
T11325	36421-36511	sentence	denotes	This is similar to the T-antigen model originally proposed by Smelkova and Borowiec (21).
T11326	36422-36426	DT	denotes	This
T11327	36427-36429	VBZ	denotes	is
T11328	36430-36437	JJ	denotes	similar
T11329	36438-36440	IN	denotes	to
T11330	36441-36444	DT	denotes	the
T11331	36455-36460	NN	denotes	model
T11332	36445-36446	NN	denotes	T
T11333	36447-36454	NN	denotes	antigen
T11334	36446-36447	HYPH	denotes	-
T11335	36461-36471	RB	denotes	originally
T11336	36472-36480	VBN	denotes	proposed
T11337	36481-36483	IN	denotes	by
T11338	36484-36492	NNP	denotes	Smelkova
T11339	36493-36496	CC	denotes	and
T11340	36497-36505	NNP	denotes	Borowiec
T11341	36506-36507	-LRB-	denotes	(
T11342	36507-36509	CD	denotes	21
T11343	36509-36510	-RRB-	denotes	)
T11344	36510-36511	.	denotes	.
T11345	36511-36700	sentence	denotes	We propose that, only when both hexamers are activated by the interacting thymine-rich sequences present within the melted region, the Mcm helicase is mobilized and initiation takes place.
T11346	36512-36514	PRP	denotes	We
T11347	36515-36522	VBP	denotes	propose
T11348	36523-36527	IN	denotes	that
T11349	36663-36672	VBN	denotes	mobilized
T11350	36527-36529	,	denotes	,
T11351	36529-36533	RB	denotes	only
T11352	36557-36566	VBN	denotes	activated
T11353	36534-36538	WRB	denotes	when
T11354	36539-36543	DT	denotes	both
T11355	36544-36552	NNS	denotes	hexamers
T11356	36553-36556	VBP	denotes	are
T11357	36567-36569	IN	denotes	by
T11358	36570-36573	DT	denotes	the
T11359	36599-36608	NNS	denotes	sequences
T11360	36574-36585	VBG	denotes	interacting
T11361	36586-36593	NN	denotes	thymine
T11362	36594-36598	JJ	denotes	rich
T11363	36593-36594	HYPH	denotes	-
T11364	36609-36616	JJ	denotes	present
T11365	36617-36623	IN	denotes	within
T11366	36624-36627	DT	denotes	the
T11367	36635-36641	NN	denotes	region
T11368	36628-36634	VBN	denotes	melted
T11369	36641-36643	,	denotes	,
T11370	36643-36646	DT	denotes	the
T11371	36651-36659	NN	denotes	helicase
T11372	36647-36650	NN	denotes	Mcm
T11373	36660-36662	VBZ	denotes	is
T11374	36673-36676	CC	denotes	and
T11375	36677-36687	NN	denotes	initiation
T11376	36688-36693	VBZ	denotes	takes
T11377	36694-36699	NN	denotes	place
T11378	36699-36700	.	denotes	.
T11379	36700-36829	sentence	denotes	A crucial question is how these double hexameric helicase complexes are generated and turn into active helicases at the origins.
T11380	36701-36702	DT	denotes	A
T11381	36711-36719	NN	denotes	question
T11382	36703-36710	JJ	denotes	crucial
T11383	36720-36722	VBZ	denotes	is
T11384	36723-36726	WRB	denotes	how
T11385	36773-36782	VBN	denotes	generated
T11386	36727-36732	DT	denotes	these
T11387	36759-36768	NNS	denotes	complexes
T11388	36733-36739	RB	denotes	double
T11389	36740-36749	JJ	denotes	hexameric
T11390	36750-36758	NN	denotes	helicase
T11391	36769-36772	VBP	denotes	are
T11392	36783-36786	CC	denotes	and
T11393	36787-36791	VB	denotes	turn
T11394	36792-36796	IN	denotes	into
T11395	36797-36803	JJ	denotes	active
T11396	36804-36813	NNS	denotes	helicases
T11397	36814-36816	IN	denotes	at
T11398	36817-36820	DT	denotes	the
T11399	36821-36828	NNS	denotes	origins
T11400	36828-36829	.	denotes	.
T11401	36829-37083	sentence	denotes	This process would involve melting of duplex DNA, which may be facilitated by binding of preRC components in the context of proper chromatin structures, or by other unwinding factors including a topoisomerase or a newly identified Mcm8 helicase (34–36).
T11402	36830-36834	DT	denotes	This
T11403	36835-36842	NN	denotes	process
T11404	36849-36856	VB	denotes	involve
T11405	36843-36848	MD	denotes	would
T11406	36857-36864	NN	denotes	melting
T11407	36865-36867	IN	denotes	of
T11408	36868-36874	NN	denotes	duplex
T11409	36875-36878	NN	denotes	DNA
T11410	36878-36880	,	denotes	,
T11411	36880-36885	WDT	denotes	which
T11412	36893-36904	VBN	denotes	facilitated
T11413	36886-36889	MD	denotes	may
T11414	36890-36892	VB	denotes	be
T11415	36905-36907	IN	denotes	by
T11416	36908-36915	NN	denotes	binding
T11417	36916-36918	IN	denotes	of
T11418	36919-36924	NN	denotes	preRC
T11419	36925-36935	NNS	denotes	components
T11420	36936-36938	IN	denotes	in
T11421	36939-36942	DT	denotes	the
T11422	36943-36950	NN	denotes	context
T11423	36951-36953	IN	denotes	of
T11424	36954-36960	JJ	denotes	proper
T11425	36971-36981	NNS	denotes	structures
T11426	36961-36970	NN	denotes	chromatin
T11427	36981-36983	,	denotes	,
T11428	36983-36985	CC	denotes	or
T11429	36986-36988	IN	denotes	by
T11430	36989-36994	JJ	denotes	other
T11431	37005-37012	NNS	denotes	factors
T11432	36995-37004	JJ	denotes	unwinding
T11433	37013-37022	VBG	denotes	including
T11434	37023-37024	DT	denotes	a
T11435	37025-37038	NN	denotes	topoisomerase
T11436	37039-37041	CC	denotes	or
T11437	37042-37043	DT	denotes	a
T11438	37066-37074	NN	denotes	helicase
T11439	37044-37049	RB	denotes	newly
T11440	37050-37060	VBN	denotes	identified
T11441	37061-37065	NN	denotes	Mcm8
T11442	37075-37076	-LRB-	denotes	(
T11443	37076-37078	CD	denotes	34
T11444	37078-37079	SYM	denotes	–
T11445	37079-37081	CD	denotes	36
T11446	37081-37082	-RRB-	denotes	)
T11447	37082-37083	.	denotes	.
T11448	37083-37231	sentence	denotes	It may also require association of fork-assisting proteins such as Cdc45 (37,38) as well as phosphorylation events by Cdk and Cdc7 kinases (39,40).
T11449	37084-37086	PRP	denotes	It
T11450	37096-37103	VB	denotes	require
T11451	37087-37090	MD	denotes	may
T11452	37091-37095	RB	denotes	also
T11453	37104-37115	NN	denotes	association
T11454	37116-37118	IN	denotes	of
T11455	37119-37123	NN	denotes	fork
T11456	37124-37133	VBG	denotes	assisting
T11457	37123-37124	HYPH	denotes	-
T11458	37134-37142	NN	denotes	proteins
T11459	37143-37147	JJ	denotes	such
T11460	37148-37150	IN	denotes	as
T11461	37151-37156	NN	denotes	Cdc45
T11462	37157-37158	-LRB-	denotes	(
T11463	37161-37163	CD	denotes	38
T11464	37158-37160	CD	denotes	37
T11465	37160-37161	,	denotes	,
T11466	37163-37164	-RRB-	denotes	)
T11467	37165-37167	RB	denotes	as
T11468	37173-37175	IN	denotes	as
T11469	37168-37172	RB	denotes	well
T11470	37176-37191	NN	denotes	phosphorylation
T11471	37192-37198	NNS	denotes	events
T11472	37199-37201	IN	denotes	by
T11473	37202-37205	NN	denotes	Cdk
T11474	37215-37222	NNS	denotes	kinases
T11475	37206-37209	CC	denotes	and
T11476	37210-37214	NN	denotes	Cdc7
T11477	37223-37224	-LRB-	denotes	(
T11478	37227-37229	CD	denotes	40
T11479	37224-37226	CD	denotes	39
T11480	37226-37227	,	denotes	,
T11481	37229-37230	-RRB-	denotes	)
T11482	37230-37231	.	denotes	.
T11483	37231-37328	sentence	denotes	One unexpected finding of this study is low helicase activity of Mcm4/6/7 on GC-rich duplex DNA.
T11484	37232-37235	CD	denotes	One
T11485	37247-37254	NN	denotes	finding
T11486	37236-37246	JJ	denotes	unexpected
T11487	37269-37271	VBZ	denotes	is
T11488	37255-37257	IN	denotes	of
T11489	37258-37262	DT	denotes	this
T11490	37263-37268	NN	denotes	study
T11491	37272-37275	JJ	denotes	low
T11492	37285-37293	NN	denotes	activity
T11493	37276-37284	NN	denotes	helicase
T11494	37294-37296	IN	denotes	of
T11495	37297-37301	NN	denotes	Mcm4
T11496	37301-37302	HYPH	denotes	/
T11497	37302-37303	CD	denotes	6
T11498	37303-37304	HYPH	denotes	/
T11499	37304-37305	CD	denotes	7
T11500	37306-37308	IN	denotes	on
T11501	37309-37311	NN	denotes	GC
T11502	37312-37316	JJ	denotes	rich
T11503	37311-37312	HYPH	denotes	-
T11504	37324-37327	NN	denotes	DNA
T11505	37317-37323	NN	denotes	duplex
T11506	37327-37328	.	denotes	.
T11507	37328-37513	sentence	denotes	This, in conjunction with the low processivity of Mcm4/6/7 helicase, strongly indicates that the replicative helicase at the replication forks would require more than Mcm4/6/7 complex.
T11508	37329-37333	DT	denotes	This
T11509	37407-37416	VBZ	denotes	indicates
T11510	37333-37335	,	denotes	,
T11511	37335-37337	IN	denotes	in
T11512	37338-37349	NN	denotes	conjunction
T11513	37350-37354	IN	denotes	with
T11514	37355-37358	DT	denotes	the
T11515	37363-37375	NN	denotes	processivity
T11516	37359-37362	JJ	denotes	low
T11517	37376-37378	IN	denotes	of
T11518	37379-37383	NN	denotes	Mcm4
T11519	37388-37396	NN	denotes	helicase
T11520	37383-37384	HYPH	denotes	/
T11521	37384-37385	CD	denotes	6
T11522	37385-37386	HYPH	denotes	/
T11523	37386-37387	CD	denotes	7
T11524	37396-37398	,	denotes	,
T11525	37398-37406	RB	denotes	strongly
T11526	37417-37421	IN	denotes	that
T11527	37478-37485	VB	denotes	require
T11528	37422-37425	DT	denotes	the
T11529	37438-37446	NN	denotes	helicase
T11530	37426-37437	JJ	denotes	replicative
T11531	37447-37449	IN	denotes	at
T11532	37450-37453	DT	denotes	the
T11533	37466-37471	NNS	denotes	forks
T11534	37454-37465	NN	denotes	replication
T11535	37472-37477	MD	denotes	would
T11536	37486-37490	JJR	denotes	more
T11537	37491-37495	IN	denotes	than
T11538	37496-37500	NN	denotes	Mcm4
T11539	37505-37512	NN	denotes	complex
T11540	37500-37501	HYPH	denotes	/
T11541	37501-37502	CD	denotes	6
T11542	37502-37503	HYPH	denotes	/
T11543	37503-37504	CD	denotes	7
T11544	37512-37513	.	denotes	.
T11545	37513-37852	sentence	denotes	During the processive unwinding of duplex DNA replication forks, Mcm4/6/7 may be further stimulated by interaction with other proteins, including remaining Mcm subunits, Cdc45 (37,38), GINS (41,42), DNA polymerase subunits (43), Mcm8 (35,36), etc. to become a truly processive and potent helicase capable of replicating the entire genome.
T11546	37514-37520	IN	denotes	During
T11547	37603-37613	VBN	denotes	stimulated
T11548	37521-37524	DT	denotes	the
T11549	37536-37545	NN	denotes	unwinding
T11550	37525-37535	JJ	denotes	processive
T11551	37546-37548	IN	denotes	of
T11552	37549-37555	NN	denotes	duplex
T11553	37572-37577	NNS	denotes	forks
T11554	37556-37559	NN	denotes	DNA
T11555	37560-37571	NN	denotes	replication
T11556	37577-37579	,	denotes	,
T11557	37579-37583	NN	denotes	Mcm4
T11558	37583-37584	HYPH	denotes	/
T11559	37584-37585	CD	denotes	6
T11560	37585-37586	HYPH	denotes	/
T11561	37586-37587	CD	denotes	7
T11562	37588-37591	MD	denotes	may
T11563	37592-37594	VB	denotes	be
T11564	37595-37602	RB	denotes	further
T11565	37614-37616	IN	denotes	by
T11566	37617-37628	NN	denotes	interaction
T11567	37629-37633	IN	denotes	with
T11568	37634-37639	JJ	denotes	other
T11569	37640-37648	NN	denotes	proteins
T11570	37648-37650	,	denotes	,
T11571	37650-37659	VBG	denotes	including
T11572	37660-37669	VBG	denotes	remaining
T11573	37674-37682	NNS	denotes	subunits
T11574	37670-37673	NN	denotes	Mcm
T11575	37682-37684	,	denotes	,
T11576	37684-37689	NN	denotes	Cdc45
T11577	37690-37691	-LRB-	denotes	(
T11578	37694-37696	CD	denotes	38
T11579	37691-37693	CD	denotes	37
T11580	37693-37694	,	denotes	,
T11581	37696-37697	-RRB-	denotes	)
T11582	37697-37699	,	denotes	,
T11583	37699-37703	NN	denotes	GINS
T11584	37704-37705	-LRB-	denotes	(
T11585	37708-37710	CD	denotes	42
T11586	37705-37707	CD	denotes	41
T11587	37707-37708	,	denotes	,
T11588	37710-37711	-RRB-	denotes	)
T11589	37711-37713	,	denotes	,
T11590	37713-37716	NN	denotes	DNA
T8823	28235-28246	NN	denotes	elucidation
T8824	28247-28249	IN	denotes	of
T8825	28250-28253	WRB	denotes	how
T8826	28280-28288	VBP	denotes	interact
T8827	28254-28257	DT	denotes	the
T8828	28270-28279	NNS	denotes	helicases
T8829	28258-28269	JJ	denotes	replicative
T8830	28289-28293	IN	denotes	with
T8831	28294-28297	NN	denotes	DNA
T8832	28298-28307	NN	denotes	substrate
T8833	28308-28311	CC	denotes	and
T8834	28312-28315	WRB	denotes	how
T8835	28325-28334	VBN	denotes	activated
T8836	28316-28320	PRP	denotes	they
T11597	37743-37747	NN	denotes	Mcm8
T11598	37748-37749	-LRB-	denotes	(
T11599	37752-37754	CD	denotes	36
T11600	37749-37751	CD	denotes	35
T11601	37751-37752	,	denotes	,
T11602	37754-37755	-RRB-	denotes	)
T11603	37755-37757	,	denotes	,
T11604	37757-37761	FW	denotes	etc.
T11605	37762-37764	TO	denotes	to
T11606	37765-37771	VB	denotes	become
T11607	37772-37773	DT	denotes	a
T11608	37802-37810	NN	denotes	helicase
T11609	37774-37779	RB	denotes	truly
T11610	37780-37790	JJ	denotes	processive
T11611	37791-37794	CC	denotes	and
T11612	37795-37801	JJ	denotes	potent
T11613	37811-37818	JJ	denotes	capable
T11614	37819-37821	IN	denotes	of
T11615	37822-37833	VBG	denotes	replicating
T11616	37834-37837	DT	denotes	the
T11617	37845-37851	NN	denotes	genome
T11618	37838-37844	JJ	denotes	entire
T11619	37851-37852	.	denotes	.
T8790	28071-28074	NN	denotes	DNA
T8791	28075-28083	NN	denotes	helicase
T8792	28084-28086	VBZ	denotes	is
T8793	28087-28088	DT	denotes	a
T8794	28097-28103	NN	denotes	factor
T8795	28089-28096	JJ	denotes	central
T8796	28104-28107	IN	denotes	for
T8797	28108-28117	NN	denotes	operation
T8798	28118-28120	IN	denotes	of
T8799	28121-28132	NN	denotes	replication
T8800	28133-28138	NNS	denotes	forks
T8801	28138-28139	.	denotes	.
T8802	28139-28219	sentence	denotes	It also plays a crucial role in the initiation step at the replication origins.
T8803	28140-28142	PRP	denotes	It
T8804	28148-28153	VBZ	denotes	plays
T8805	28143-28147	RB	denotes	also
T8806	28154-28155	DT	denotes	a
T8807	28164-28168	NN	denotes	role
T8808	28156-28163	JJ	denotes	crucial
T8809	28169-28171	IN	denotes	in
T8810	28172-28175	DT	denotes	the
T8811	28187-28191	NN	denotes	step
T8812	28176-28186	NN	denotes	initiation
T8813	28192-28194	IN	denotes	at
T8814	28195-28198	DT	denotes	the
T8815	28211-28218	NNS	denotes	origins
T8816	28199-28210	NN	denotes	replication
T8817	28218-28219	.	denotes	.
T8818	28219-28417	sentence	denotes	Therefore, the elucidation of how the replicative helicases interact with DNA substrate and how they are activated is crucial for understanding the molecular basis of initiation of DNA replication.
T8819	28220-28229	RB	denotes	Therefore
T8820	28335-28337	VBZ	denotes	is
T8821	28229-28231	,	denotes	,
T8822	28231-28234	DT	denotes	the
T8837	28321-28324	VBP	denotes	are
T142	0-3	NN	denotes	DNA
T11591	37728-37736	NNS	denotes	subunits
T11592	37717-37727	NN	denotes	polymerase
T11593	37737-37738	-LRB-	denotes	(
T11594	37738-37740	CD	denotes	43
T11595	37740-37741	-RRB-	denotes	)
T11596	37741-37743	,	denotes	,
R4	T143	T145	nmod	binding,actions
R5	T146	T143	cc	and,binding
R6	T147	T143	conj	helicase,binding
R7	T148	T145	prep	of,actions
R8	T149	T150	nmod	mouse,helicase
R9	T150	T148	pobj	helicase,of
R10	T151	T150	nmod	MCM4,helicase
R11	T152	T151	punct	/,MCM4
R12	T153	T151	nummod	6,MCM4
R13	T154	T151	punct	/,MCM4
R14	T155	T151	nummod	7,MCM4
R15	T158	T159	nsubj	Helicases,play
R16	T160	T161	amod	central,roles
R17	T161	T159	dobj	roles,play
R18	T162	T159	prep	in,play
R19	T163	T162	pobj	initiation,in
R20	T164	T163	cc	and,initiation
R21	T165	T163	conj	elongation,initiation
R22	T166	T163	prep	of,initiation
R23	T167	T168	compound	DNA,replication
R24	T168	T166	pobj	replication,of
R25	T169	T159	punct	.,play
R26	T171	T172	nsubj	We,reported
R27	T173	T172	advmod	previously,reported
R28	T174	T175	mark	that,activated
R29	T175	T172	ccomp	activated,reported
R30	T176	T177	nmod	helicase,activities
R31	T177	T175	nsubjpass	activities,activated
R32	T178	T176	cc	and,helicase
R33	T179	T176	conj	ATPase,helicase
R34	T180	T177	prep	of,activities
R35	T181	T182	det	the,complex
R36	T182	T180	pobj	complex,of
R37	T183	T182	amod	mammalian,complex
R38	T184	T182	nmod	Mcm4,complex
R39	T185	T184	punct	/,Mcm4
R40	T291	T274	prep	in,play
R41	T292	T293	amod	efficient,unwinding
R42	T293	T291	pobj	unwinding,in
R43	T294	T293	prep	of,unwinding
R44	T295	T296	det	the,bubble
R45	T296	T294	pobj	bubble,of
R46	T297	T256	punct	.,results
R47	T299	T300	nsubj	Mcm4,binds
R48	T301	T299	punct	/,Mcm4
R49	T302	T299	nummod	6,Mcm4
R50	T303	T299	punct	/,Mcm4
R51	T186	T184	nummod	6,Mcm4
R52	T187	T184	punct	/,Mcm4
R53	T188	T184	nummod	7,Mcm4
R54	T304	T299	nummod	7,Mcm4
R55	T189	T175	auxpass	are,activated
R56	T190	T175	advmod	specifically,activated
R57	T191	T175	agent	by,activated
R58	T305	T300	cc	and,binds
R59	T192	T193	npadvmod	thymine,rich
R60	T193	T195	amod	rich,DNA
R61	T306	T300	conj	unwinds,binds
R62	T194	T193	punct	-,rich
R63	T307	T308	amod	various,structures
R64	T308	T306	dobj	structures,unwinds
R65	T309	T308	nmod	fork,structures
R66	T310	T309	cc	and,fork
R67	T195	T191	pobj	DNA,by
R68	T311	T309	conj	extension,fork
R69	T196	T197	amod	single,stranded
R70	T197	T195	amod	stranded,DNA
R71	T312	T308	acl	carrying,structures
R72	T198	T197	punct	-,stranded
R73	T199	T172	punct	.,reported
R74	T313	T314	det	a,DNA
R75	T201	T202	advmod	Here,examined
R76	T314	T312	dobj	DNA,carrying
R77	T203	T202	punct	", ",examined
R78	T315	T316	amod	single,stranded
R79	T204	T202	nsubj	we,examined
R80	T205	T206	poss	its,preference
R81	T316	T314	amod	stranded,DNA
R82	T206	T202	dobj	preference,examined
R83	T207	T206	compound	substrate,preference
R84	T208	T206	cc	and,preference
R85	T317	T316	punct	-,stranded
R86	T209	T210	compound	helicase,actions
R87	T210	T206	conj	actions,preference
R88	T318	T319	nummod	3,tail
R89	T211	T202	advcl	using,examined
R90	T212	T213	amod	various,DNAs
R91	T319	T314	compound	tail,DNA
R92	T213	T211	dobj	DNAs,using
R93	T214	T213	amod	synthetic,DNAs
R94	T320	T318	punct	′,3
R95	T215	T202	punct	.,examined
R96	T217	T218	prep	On,makes
R97	T321	T319	punct	-,tail
R98	T219	T220	det	a,substrate
R99	T322	T300	punct	.,binds
R100	T220	T217	pobj	substrate,On
R101	T324	T325	det	The,extent
R102	T221	T220	compound	bubble,substrate
R103	T222	T218	punct	", ",makes
R104	T223	T218	nsubj	Mcm4,makes
R105	T224	T223	punct	/,Mcm4
R106	T225	T223	nummod	6,Mcm4
R107	T226	T223	punct	/,Mcm4
R108	T325	T326	nsubj	extent,depends
R109	T227	T223	nummod	7,Mcm4
R110	T228	T229	amod	symmetric,contacts
R111	T327	T325	prep	of,extent
R112	T229	T218	dobj	contacts,makes
R113	T230	T229	amod	dual,contacts
R114	T231	T229	prep	with,contacts
R115	T328	T329	compound	helicase,activation
R116	T232	T233	det	the,segments
R117	T233	T231	pobj	segments,with
R118	T329	T327	pobj	activation,of
R119	T234	T235	npadvmod	5,proximal
R120	T235	T233	amod	proximal,segments
R121	T236	T234	punct	′,5
R122	T237	T235	punct	-,proximal
R123	T238	T239	nummod	25,nt
R124	T330	T326	prep	on,depends
R125	T331	T332	det	the,context
R126	T239	T233	nmod	nt,segments
R127	T332	T330	pobj	context,on
R128	T240	T241	amod	single,stranded
R129	T241	T233	amod	stranded,segments
R130	T333	T332	compound	sequence,context
R131	T242	T241	punct	-,stranded
R132	T243	T233	amod	adjacent,segments
R133	T334	T332	prep	of,context
R134	T244	T243	prep	to,adjacent
R135	T245	T246	det	the,points
R136	T335	T336	det	the,tail
R137	T246	T244	pobj	points,to
R138	T247	T246	compound	branch,points
R139	T336	T334	pobj	tail,of
R140	T248	T218	punct	", ",makes
R141	T249	T250	advmod	presumably,generating
R142	T337	T336	nummod	3,tail
R143	T250	T218	advcl	generating,makes
R144	T251	T252	amod	double,hexamers
R145	T338	T337	punct	′,3
R146	T252	T250	dobj	hexamers,generating
R147	T253	T218	punct	.,makes
R148	T339	T336	punct	-,tail
R149	T255	T256	nsubj	Loss,results
R150	T340	T326	punct	", ",depends
R151	T257	T255	prep	of,Loss
R152	T258	T259	compound	thymine,residues
R153	T259	T257	pobj	residues,of
R154	T341	T326	cc	and,depends
R155	T342	T343	det	the,level
R156	T260	T255	prep	from,Loss
R157	T261	T262	nummod	one,strand
R158	T262	T260	pobj	strand,from
R159	T343	T345	nsubjpass	level,achieved
R160	T263	T262	amod	single,strand
R161	T344	T343	amod	maximum,level
R162	T264	T262	punct	-,strand
R163	T265	T256	prep	in,results
R164	T345	T326	conj	achieved,depends
R165	T266	T267	amod	significant,decrease
R166	T267	T265	pobj	decrease,in
R167	T346	T345	auxpass	is,achieved
R168	T268	T267	prep	of,decrease
R169	T269	T270	amod	unwinding,efficacy
R170	T270	T268	pobj	efficacy,of
R171	T347	T345	agent	by,achieved
R172	T271	T256	punct	", ",results
R173	T272	T256	advcl	suggesting,results
R174	T348	T347	pobj	DNA,by
R175	T273	T274	mark	that,play
R176	T349	T348	prep	with,DNA
R177	T274	T272	ccomp	play,suggesting
R178	T350	T351	nummod	50,%
R179	T275	T276	amod	concurrent,unwinding
R180	T276	T274	nsubj	unwinding,play
R181	T351	T352	nmod	%,content
R182	T277	T276	amod	bidirectional,unwinding
R183	T278	T276	prep	by,unwinding
R184	T352	T349	pobj	content,with
R185	T279	T280	det	a,Mcm4
R186	T280	T278	pobj	Mcm4,by
R187	T281	T280	amod	single,Mcm4
R188	T353	T354	cc	or,more
R189	T282	T283	amod	double,hexameric
R190	T283	T280	amod	hexameric,Mcm4
R191	T354	T351	nummod	more,%
R192	T284	T280	punct	/,Mcm4
R193	T285	T280	nummod	6,Mcm4
R194	T355	T352	compound	thymine,content
R195	T286	T280	punct	/,Mcm4
R196	T287	T280	nummod	7,Mcm4
R197	T356	T345	punct	.,achieved
R198	T288	T274	aux	may,play
R199	T289	T290	det	a,role
R200	T358	T359	compound	Strand,displacement
R201	T290	T274	dobj	role,play
R202	T359	T360	nsubjpass	displacement,inhibited
R203	T361	T359	prep	by,displacement
R204	T362	T361	pobj	Mcm4,by
R205	T363	T362	punct	/,Mcm4
R206	T364	T362	nummod	6,Mcm4
R207	T365	T362	punct	/,Mcm4
R208	T366	T362	nummod	7,Mcm4
R209	T397	T381	advcl	suggesting,restored
R210	T398	T399	mark	that,has
R211	T399	T397	ccomp	has,suggesting
R212	T367	T360	auxpass	is,inhibited
R213	T400	T401	amod	mammalian,helicase
R214	T401	T399	nsubj	helicase,has
R215	T368	T360	punct	", ",inhibited
R216	T402	T401	nmod	Mcm4,helicase
R217	T403	T402	punct	/,Mcm4
R218	T369	T370	mark	as,increases
R219	T404	T402	nummod	6,Mcm4
R220	T405	T402	punct	/,Mcm4
R221	T406	T402	nummod	7,Mcm4
R222	T370	T360	advcl	increases,inhibited
R223	T407	T399	dobj	difficulties,has
R224	T408	T407	prep	in,difficulties
R225	T371	T372	det	the,content
R226	T409	T408	pcomp	unwinding,in
R227	T372	T370	nsubj	content,increases
R228	T410	T411	advmod	stably,paired
R229	T411	T414	amod	paired,duplex
R230	T412	T411	npadvmod	base,paired
R231	T373	T372	compound	GC,content
R232	T413	T411	punct	-,paired
R233	T414	T409	dobj	duplex,unwinding
R234	T374	T372	prep	of,content
R235	T375	T376	det	the,region
R236	T415	T381	punct	.,restored
R237	T376	T374	pobj	region,of
R238	T417	T418	advcl	Taken,reveal
R239	T377	T376	compound	duplex,region
R240	T419	T417	advmod	together,Taken
R241	T378	T360	punct	.,inhibited
R242	T380	T381	nsubj	Replacement,restored
R243	T420	T418	punct	", ",reveal
R244	T421	T422	det	these,findings
R245	T382	T380	prep	of,Replacement
R246	T422	T418	nsubj	findings,reveal
R247	T423	T424	amod	important,features
R248	T424	T418	dobj	features,reveal
R249	T383	T384	compound	cytosine,guanine
R250	T425	T424	prep	on,features
R251	T384	T386	compound	guanine,pairs
R252	T426	T427	nmod	activation,preference
R253	T427	T425	pobj	preference,on
R254	T428	T426	cc	and,activation
R255	T385	T384	punct	–,guanine
R256	T429	T426	conj	substrate,activation
R257	T430	T427	prep	of,preference
R258	T386	T382	pobj	pairs,of
R259	T431	T432	det	the,helicase
R260	T432	T430	pobj	helicase,of
R261	T387	T380	prep	with,Replacement
R262	T433	T432	amod	eukaryotic,helicase
R263	T434	T432	amod	replicative,helicase
R264	T435	T418	punct	.,reveal
R265	T388	T389	compound	cytosine,inosine
R266	T389	T391	compound	inosine,pairs
R267	T390	T389	punct	–,inosine
R268	T391	T387	pobj	pairs,with
R269	T392	T380	prep	in,Replacement
R270	T393	T394	det	the,duplex
R271	T394	T392	pobj	duplex,in
R272	T395	T381	dobj	unwinding,restored
R273	T396	T381	punct	", ",restored
R274	T142	T143	nmod	DNA,binding
R278	T757	T758	compound	DNA,helicases
R279	T758	T759	nsubj	helicases,couple
R280	T760	T761	det	the,hydrolysis
R281	T761	T759	dobj	hydrolysis,couple
R282	T762	T761	prep	of,hydrolysis
R283	T763	T764	nmod	nucleotide,triphosphate
R284	T764	T762	pobj	triphosphate,of
R285	T765	T763	cc	or,nucleotide
R286	T766	T763	conj	deoxynucleotide,nucleotide
R287	T767	T759	prep	to,couple
R288	T768	T769	compound	duplex,unwinding
R289	T769	T767	pobj	unwinding,to
R290	T770	T771	punct	(,1
R291	T771	T759	parataxis	1,couple
R292	T772	T771	punct	),1
R293	T773	T759	punct	.,couple
R294	T775	T776	nsubjpass	Coordination,required
R295	T777	T775	prep	of,Coordination
R296	T778	T779	det	a,set
R297	T779	T777	pobj	set,of
R298	T780	T779	prep	of,set
R299	T781	T780	pobj	subactivities,of
R300	T782	T781	prep	including,subactivities
R301	T783	T784	compound	nucleotide,binding
R302	T784	T782	pobj	binding,including
R303	T785	T784	punct	", ",binding
R304	T786	T787	compound	DNA,binding
R305	T787	T784	conj	binding,binding
R306	T788	T787	cc	and,binding
R307	T789	T790	compound	ATP,hydrolysis
R308	T790	T787	conj	hydrolysis,binding
R309	T791	T776	auxpass	is,required
R310	T792	T776	prep	for,required
R311	T793	T792	pobj	unwinding,for
R312	T794	T793	prep	of,unwinding
R313	T795	T796	compound	duplex,DNA
R314	T796	T794	pobj	DNA,of
R315	T797	T798	punct	(,2
R316	T798	T776	parataxis	2,required
R317	T799	T798	nummod	1,2
R318	T800	T798	punct	",",2
R319	T801	T798	punct	),2
R320	T802	T776	punct	.,required
R321	T804	T805	det	The,helicases
R322	T805	T807	nsubjpass	helicases,known
R323	T806	T805	amod	hexameric,helicases
R324	T808	T805	punct	", ",helicases
R325	T809	T810	prep	to,belongs
R326	T810	T805	relcl	belongs,helicases
R327	T811	T809	pobj	which,to
R328	T812	T813	nmod	minichromosome,maintenance
R329	T813	T814	nmod	maintenance,helicase
R330	T814	T810	nsubj	helicase,belongs
R331	T815	T813	punct	(,maintenance
R332	T816	T813	appos	Mcm,maintenance
R333	T817	T813	punct	),maintenance
R334	T818	T807	punct	", ",known
R335	T819	T807	auxpass	are,known
R336	T820	T821	aux	to,assemble
R337	T821	T807	xcomp	assemble,known
R338	T822	T823	det	a,structure
R339	T823	T821	dobj	structure,assemble
R340	T824	T825	npadvmod	ring,like
R341	T825	T823	amod	like,structure
R342	T826	T825	punct	-,like
R343	T827	T828	punct	(,3
R344	T828	T807	parataxis	3,known
R345	T829	T830	punct	–,5
R346	T830	T828	prep	5,3
R347	T831	T828	punct	),3
R348	T832	T807	punct	.,known
R349	T834	T835	mark	Whereas,composed
R350	T835	T874	advcl	composed,composed
R351	T836	T835	nsubjpass	most,composed
R352	T837	T836	prep	of,most
R353	T838	T839	compound	DNA,helicases
R354	T839	T837	pobj	helicases,of
R355	T840	T839	prep	of,helicases
R356	T841	T842	det	this,group
R357	T842	T840	pobj	group,of
R358	T843	T836	punct	", ",most
R359	T844	T836	prep	including,most
R360	T845	T846	amod	simian,virus
R361	T846	T847	nmod	virus,antigen
R362	T847	T844	pobj	antigen,including
R363	T848	T846	nummod	40,virus
R364	T849	T847	amod	large,antigen
R365	T850	T847	compound	T,antigen
R366	T851	T847	punct	-,antigen
R367	T852	T853	punct	(,6
R368	T853	T847	parataxis	6,antigen
R369	T854	T853	punct	),6
R370	T855	T847	punct	", ",antigen
R371	T856	T857	compound	Escherichia,coli
R372	T857	T858	compound	coli,DnaB
R373	T858	T847	conj	DnaB,antigen
R374	T859	T860	punct	(,7
R375	T860	T858	parataxis	7,DnaB
R376	T861	T860	punct	),7
R377	T862	T858	cc	and,DnaB
R378	T863	T864	amod	Archaeal,Mcm
R379	T864	T858	conj	Mcm,DnaB
R380	T865	T866	punct	(,9
R381	T866	T864	parataxis	9,Mcm
R382	T867	T866	nummod	4,9
R383	T868	T866	punct	",",9
R384	T869	T866	nummod	8,9
R385	T870	T866	punct	",",9
R386	T871	T866	punct	),9
R387	T872	T835	punct	", ",composed
R388	T873	T835	auxpass	are,composed
R389	T875	T835	prep	of,composed
R390	T876	T877	det	a,subunit
R391	T877	T875	pobj	subunit,of
R392	T878	T877	amod	single,subunit
R393	T879	T874	punct	", ",composed
R394	T880	T881	det	the,helicases
R395	T881	T874	nsubjpass	helicases,composed
R396	T882	T881	amod	active,helicases
R397	T883	T881	amod	eukaryotic,helicases
R398	T884	T881	compound	Mcm,helicases
R399	T885	T874	auxpass	are,composed
R400	T886	T874	prep	of,composed
R401	T887	T888	nummod	two,proteins
R403	T889	T888	amod	trimeric,proteins
R404	T890	T888	nmod	Mcm4,proteins
R405	T891	T890	punct	/,Mcm4
R406	T892	T890	nummod	6,Mcm4
R407	T893	T890	punct	/,Mcm4
R408	T894	T890	nummod	7,Mcm4
R409	T895	T896	punct	(,10
R410	T896	T874	parataxis	10,composed
R411	T897	T898	punct	–,14
R412	T898	T896	prep	14,10
R413	T899	T896	punct	),10
R414	T900	T874	punct	.,composed
R415	T902	T903	predet	All,proteins
R417	T904	T903	det	the,proteins
R418	T905	T903	nummod	six,proteins
R419	T906	T907	nmod	Mcm,2
R420	T907	T903	nummod	2,proteins
R421	T908	T909	punct	–,7
R422	T909	T907	prep	7,2
R423	T911	T912	advmod	highly,conserved
R424	T912	T913	amod	conserved,motifs
R425	T913	T910	dobj	motifs,contain
R426	T914	T915	npadvmod	DNA,dependent
R427	T915	T913	amod	dependent,motifs
R428	T916	T915	punct	-,dependent
R429	T917	T913	compound	ATPase,motifs
R430	T918	T910	prep	in,contain
R431	T919	T920	poss	their,regions
R432	T920	T918	pobj	regions,in
R433	T921	T920	amod	central,regions
R434	T922	T923	punct	(,16
R435	T923	T910	parataxis	16,contain
R436	T924	T923	nummod	15,16
R437	T925	T923	punct	",",16
R438	T926	T923	punct	),16
R439	T927	T910	punct	.,contain
R440	T929	T930	prep	Among,shown
R441	T931	T932	det	the,subcomplexes
R442	T932	T929	pobj	subcomplexes,Among
R443	T933	T932	amod	several,subcomplexes
R444	T934	T932	amod	stable,subcomplexes
R445	T935	T936	dep	which,generate
R446	T936	T932	relcl	generate,subcomplexes
R447	T937	T938	compound	MCM,proteins
R448	T938	T936	nsubj	proteins,generate
R449	T939	T936	aux	can,generate
R450	T940	T930	punct	", ",shown
R451	T941	T942	advmod	only,complex
R453	T943	T942	det	the,complex
R454	T944	T942	nmod	Mcm4,complex
R455	T945	T944	punct	/,Mcm4
R456	T946	T944	nummod	6,Mcm4
R457	T947	T944	punct	/,Mcm4
R458	T948	T944	nummod	7,Mcm4
R459	T949	T930	aux	has,shown
R460	T950	T930	auxpass	been,shown
R461	T951	T952	aux	to,possess
R462	T952	T930	xcomp	possess,shown
R463	T953	T954	det	an,activity
R464	T954	T952	dobj	activity,possess
R465	T955	T954	amod	intrinsic,activity
R466	T956	T957	compound	DNA,helicase
R467	T957	T954	compound	helicase,activity
R468	T958	T959	punct	(,10
R469	T959	T930	parataxis	10,shown
R470	T960	T961	punct	–,14
R471	T961	T959	prep	14,10
R472	T962	T959	punct	",",10
R473	T963	T959	appos	17,10
R474	T964	T965	punct	–,18
R475	T965	T963	prep	18,17
R476	T966	T959	punct	),10
R477	T967	T930	punct	.,shown
R478	T969	T970	mark	While,make
R480	T971	T972	nmod	Mcm4,proteins
R481	T972	T970	nsubj	proteins,make
R482	T973	T971	punct	", ",Mcm4
R483	T974	T971	conj	Mcm6,Mcm4
R484	T975	T974	cc	and,Mcm6
R485	T976	T974	conj	Mcm7,Mcm6
R486	T978	T979	amod	distinct,contribution
R487	T979	T970	dobj	contribution,make
R488	T980	T979	prep	to,contribution
R489	T981	T982	poss	its,activity
R490	T982	T980	pobj	activity,to
R491	T983	T982	compound	helicase,activity
R492	T984	T985	punct	(,11
R493	T985	T970	parataxis	11,make
R494	T986	T987	punct	–,13
R495	T987	T985	prep	13,11
R496	T988	T985	punct	),11
R497	T989	T977	punct	", ",inhibit
R498	T990	T977	nsubj	Mcm2,inhibit
R499	T991	T990	cc	or,Mcm2
R500	T992	T990	conj	Mcm3,Mcm2
R501	T993	T992	punct	/,Mcm3
R502	T994	T992	nummod	5,Mcm3
R503	T995	T996	det	the,activity
R504	T996	T977	dobj	activity,inhibit
R505	T997	T996	compound	helicase,activity
R506	T998	T996	prep	of,activity
R507	T999	T1000	det	the,complex
R508	T1000	T998	pobj	complex,of
R509	T1001	T1000	nmod	Mcm4,complex
R510	T1002	T1001	punct	/,Mcm4
R511	T1003	T1001	nummod	6,Mcm4
R512	T1004	T1001	punct	/,Mcm4
R513	T1005	T1001	nummod	7,Mcm4
R514	T1006	T977	prep	by,inhibit
R515	T1007	T1006	pcomp	converting,by
R516	T1008	T1009	poss	its,structure
R517	T1009	T1007	dobj	structure,converting
R518	T1010	T1009	amod	double,structure
R519	T1011	T1009	compound	trimer,structure
R520	T1012	T1007	prep	into,converting
R521	T1013	T1014	det	a,heterotetramer
R522	T1014	T1012	pobj	heterotetramer,into
R523	T1015	T1014	cc	or,heterotetramer
R524	T1016	T1014	conj	heteropentamer,heterotetramer
R525	T1017	T1007	punct	", ",converting
R526	T1018	T1007	advmod	respectively,converting
R527	T1019	T1020	punct	(,11
R528	T1020	T1007	parataxis	11,converting
R529	T1021	T1020	nummod	3,11
R530	T1022	T1020	punct	",",11
R531	T1023	T1020	punct	),11
R532	T1024	T977	punct	.,inhibit
R533	T1026	T1027	compound	Chromatin,assays
R534	T1027	T1029	nsubj	assays,suggested
R535	T1028	T1027	compound	immunoprecipitation,assays
R536	T1030	T1027	cc	and,assays
R537	T1031	T1032	amod	genetic,characterization
R538	T1032	T1027	conj	characterization,assays
R539	T1033	T1027	prep	in,assays
R540	T1034	T1035	compound	Saccharomyces,cerevisiae
R541	T1035	T1033	pobj	cerevisiae,in
R542	T1036	T1029	advmod	strongly,suggested
R543	T1037	T1038	mark	that,involved
R544	T1038	T1029	ccomp	involved,suggested
R545	T1039	T1038	nsubjpass	Mcm,involved
R546	T1040	T1038	auxpass	is,involved
R547	T1041	T1042	preconj	not,in
R548	T1042	T1038	prep	in,involved
R549	T1043	T1041	advmod	only,not
R550	T1044	T1042	pobj	initiation,in
R551	T1045	T1042	cc	but,in
R552	T1046	T1045	advmod	also,but
R553	T1047	T1042	conj	in,in
R554	T1048	T1049	det	the,stage
R555	T1049	T1047	pobj	stage,in
R556	T1050	T1051	compound	DNA,elongation
R557	T1051	T1049	compound	elongation,stage
R558	T1052	T1051	compound	chain,elongation
R559	T1053	T1038	prep	as,involved
R560	T1054	T1055	det	a,helicase
R561	T1055	T1053	pobj	helicase,as
R562	T1056	T1055	amod	replicative,helicase
R563	T1057	T1058	punct	(,20
R564	T1058	T1029	parataxis	20,suggested
R565	T1059	T1058	nummod	19,20
R566	T1060	T1058	punct	",",20
R567	T1061	T1058	punct	),20
R568	T1062	T1029	punct	.,suggested
R569	T1064	T1065	advcl	Consistent,stimulated
R570	T1066	T1064	prep	with,Consistent
R571	T1067	T1068	det	this,notion
R572	T1068	T1066	pobj	notion,with
R573	T1069	T1065	punct	", ",stimulated
R574	T1070	T1071	det	the,processivity
R575	T1071	T1065	nsubjpass	processivity,stimulated
R576	T1072	T1071	prep	of,processivity
R577	T1073	T1074	det	the,complexes
R578	T1074	T1072	pobj	complexes,of
R579	T1075	T1076	nmod	Schizosaccharomyces,pombe
R580	T1076	T1074	nmod	pombe,complexes
R581	T1077	T1076	cc	and,pombe
R582	T1078	T1079	compound	mouse,Mcm4
R583	T1079	T1076	conj	Mcm4,pombe
R584	T1080	T1079	punct	/,Mcm4
R585	T1081	T1079	nummod	6,Mcm4
R586	T1082	T1079	punct	/,Mcm4
R587	T1083	T1079	nummod	7,Mcm4
R588	T1084	T1065	auxpass	is,stimulated
R589	T1085	T1065	advmod	significantly,stimulated
R590	T1086	T1065	prep	on,stimulated
R591	T1087	T1088	amod	forked,DNA
R592	T1088	T1089	compound	DNA,structures
R593	T1089	T1086	pobj	structures,on
R594	T1090	T1065	cc	and,stimulated
R595	T1091	T1092	nsubj	it,unwind
R596	T1092	T1065	conj	unwind,stimulated
R597	T1093	T1092	aux	can,unwind
R598	T1094	T1095	compound	duplex,DNA
R599	T1095	T1092	dobj	DNA,unwind
R600	T1096	T1095	prep	of,DNA
R601	T1097	T1098	quantmod	400,500
R602	T1098	T1100	nummod	500,bp
R603	T1099	T1098	punct	–,500
R604	T1100	T1096	pobj	bp,of
R605	T1101	T1102	punct	(,14
R606	T1102	T1092	parataxis	14,unwind
R607	T1103	T1102	nummod	13,14
R608	T1104	T1102	punct	",",14
R609	T1105	T1102	punct	),14
R610	T1106	T1092	punct	.,unwind
R611	T1108	T1109	nsubj	Mcm4,binds
R612	T1110	T1108	punct	/,Mcm4
R613	T1111	T1108	nummod	6,Mcm4
R614	T1112	T1108	punct	/,Mcm4
R615	T1113	T1108	nummod	7,Mcm4
R616	T1114	T1109	prep	to,binds
R617	T1115	T1116	nmod	fork,structures
R618	T1116	T1114	pobj	structures,to
R619	T1117	T1115	cc	and,fork
R620	T1118	T1115	conj	bubble,fork
R621	T1119	T1109	prep	in,binds
R622	T1120	T1121	det	an,manner
R623	T1121	T1119	pobj	manner,in
R624	T1122	T1123	npadvmod	ATP,dependent
R625	T1123	T1121	amod	dependent,manner
R626	T1124	T1123	punct	-,dependent
R627	T1125	T1109	punct	", ",binds
R628	T1126	T1109	cc	and,binds
R629	T1127	T1109	conj	generates,binds
R630	T1128	T1129	det	a,complex
R631	T1129	T1127	dobj	complex,generates
R632	T1130	T1131	amod	double,hexameric
R633	T1131	T1129	amod	hexameric,complex
R634	T1132	T1131	punct	-,hexameric
R635	T1133	T1127	punct	", ",generates
R636	T1134	T1135	mark	as,shown
R637	T1135	T1127	advcl	shown,generates
R638	T1136	T1135	auxpass	was,shown
R639	T1137	T1135	prep	for,shown
R640	T1138	T1139	compound	T,antigen
R641	T1139	T1137	pobj	antigen,for
R642	T1140	T1139	punct	-,antigen
R643	T1141	T1142	punct	(,21
R644	T1142	T1135	parataxis	21,shown
R645	T1143	T1142	nummod	13,21
R646	T1144	T1142	punct	",",21
R647	T1145	T1142	nummod	14,21
R648	T1146	T1142	punct	",",21
R649	T1147	T1142	punct	),21
R650	T1148	T1109	punct	.,binds
R651	T1150	T1151	advmod	Recently,reported
R652	T1152	T1151	punct	", ",reported
R653	T1153	T1151	nsubj	we,reported
R654	T1154	T1155	mark	that,activated
R655	T1155	T1151	ccomp	activated,reported
R656	T1156	T1157	det	the,activities
R657	T1157	T1155	nsubjpass	activities,activated
R658	T1158	T1157	nmod	helicase,activities
R659	T1159	T1158	cc	and,helicase
R660	T1160	T1161	compound	ATP,hydrolysis
R661	T1161	T1158	conj	hydrolysis,helicase
R662	T1162	T1157	prep	of,activities
R663	T1163	T1164	amod	mammalian,Mcm4
R664	T1164	T1162	pobj	Mcm4,of
R665	T1165	T1164	punct	/,Mcm4
R666	T1166	T1164	nummod	6,Mcm4
R667	T1167	T1164	punct	/,Mcm4
R668	T1168	T1164	nummod	7,Mcm4
R669	T1169	T1155	auxpass	are,activated
R670	T1170	T1155	advmod	specifically,activated
R671	T1171	T1155	agent	by,activated
R672	T1172	T1173	amod	single,stranded
R673	T1173	T1175	amod	stranded,DNA
R674	T1174	T1173	punct	-,stranded
R675	T1175	T1171	pobj	DNA,by
R676	T1176	T1175	acl	containing,DNA
R677	T1177	T1176	dobj	stretches,containing
R678	T1178	T1177	prep	of,stretches
R679	T1179	T1180	compound	thymine,residues
R680	T1180	T1178	pobj	residues,of
R681	T1181	T1151	cc	and,reported
R682	T1182	T1151	conj	proposed,reported
R683	T1183	T1184	det	a,model
R684	T1184	T1182	dobj	model,proposed
R685	T1185	T1184	amod	novel,model
R686	T1186	T1187	mark	that,play
R687	T1187	T1184	acl	play,model
R688	T1188	T1187	nsubj	Mcm,play
R689	T1189	T1187	aux	may,play
R690	T1190	T1191	det	a,role
R691	T1191	T1187	dobj	role,play
R692	T1192	T1191	amod	crucial,role
R693	T1193	T1187	prep	in,play
R694	T1194	T1193	pobj	selection,in
R695	T1195	T1194	prep	of,selection
R696	T1196	T1197	compound	replication,origins
R697	T1197	T1195	pobj	origins,of
R698	T1198	T1187	prep	in,play
R699	T1199	T1200	amod	higher,eukaryotes
R700	T1200	T1198	pobj	eukaryotes,in
R701	T1201	T1202	punct	(,13
R702	T1202	T1182	parataxis	13,proposed
R703	T1203	T1202	punct	),13
R704	T1204	T1151	punct	.,reported
R705	T1206	T1207	prep	In,conducted
R706	T1208	T1209	det	this,report
R707	T1209	T1206	pobj	report,In
R708	T1210	T1207	punct	", ",conducted
R709	T1211	T1207	prep	in,conducted
R710	T1212	T1211	pobj	order,in
R711	T1213	T1214	aux	to,clarify
R712	T1214	T1212	acl	clarify,order
R713	T1215	T1216	det	the,mode
R714	T1216	T1214	dobj	mode,clarify
R715	T1217	T1216	prep	of,mode
R716	T1218	T1217	pobj	action,of
R717	T1219	T1216	prep	of,mode
R718	T1220	T1221	det	the,helicase
R719	T1221	T1219	pobj	helicase,of
R720	T1222	T1221	compound	Mcm,helicase
R721	T1223	T1214	cc	and,clarify
R722	T1224	T1214	conj	obtain,clarify
R723	T1225	T1224	dobj	insight,obtain
R724	T1226	T1225	prep	into,insight
R725	T1227	T1228	advmod	how,function
R726	T1228	T1226	pcomp	function,into
R727	T1229	T1228	nsubj	it,function
R728	T1230	T1228	aux	may,function
R729	T1231	T1228	prep	at,function
R730	T1232	T1233	det	the,forks
R731	T1233	T1231	pobj	forks,at
R732	T1234	T1233	compound	replication,forks
R733	T1235	T1236	advmod	in,vivo
R734	T1236	T1228	advmod	vivo,function
R735	T1237	T1207	punct	", ",conducted
R736	T1238	T1207	nsubj	we,conducted
R737	T1239	T1207	aux	have,conducted
R738	T1240	T1241	amod	detailed,analyses
R739	T1241	T1207	dobj	analyses,conducted
R740	T1242	T1241	prep	of,analyses
R741	T1243	T1244	compound	helicase,action
R742	T1244	T1242	pobj	action,of
R743	T1245	T1244	cc	and,action
R744	T1246	T1247	compound	DNA,binding
R745	T1247	T1244	conj	binding,action
R746	T1248	T1244	prep	of,action
R747	T1249	T1250	nmod	mouse,helicase
R748	T1250	T1248	pobj	helicase,of
R749	T1251	T1250	nmod	Mcm4,helicase
R750	T1252	T1251	punct	/,Mcm4
R751	T1253	T1251	nummod	6,Mcm4
R752	T1254	T1251	punct	/,Mcm4
R753	T1255	T1251	nummod	7,Mcm4
R754	T1256	T1207	advcl	using,conducted
R755	T1257	T1258	amod	various,DNAs
R756	T1258	T1256	dobj	DNAs,using
R757	T1259	T1258	amod	forked,DNAs
R758	T1260	T1259	cc	and,forked
R759	T1261	T1262	compound	bubble,substrate
R760	T1262	T1259	conj	substrate,forked
R761	T1263	T1207	punct	.,conducted
R762	T1265	T1266	nsubj	We,addressed
R763	T1267	T1266	advmod	specifically,addressed
R764	T1268	T1269	compound	sequence,requirement
R765	T1269	T1266	dobj	requirement,addressed
R766	T1270	T1269	prep	for,requirement
R767	T1271	T1272	det	the,activation
R768	T1272	T1270	pobj	activation,for
R769	T1273	T1274	compound	Mcm,helicase
R770	T1274	T1272	compound	helicase,activation
R771	T1275	T1272	punct	", ",activation
R772	T1276	T1272	conj	mode,activation
R773	T1277	T1276	prep	of,mode
R774	T1278	T1277	pobj	interaction,of
R775	T1279	T1278	prep	with,interaction
R776	T1280	T1281	compound	DNA,substrates
R777	T1281	T1279	pobj	substrates,with
R778	T1282	T1276	cc	and,mode
R779	T1283	T1284	mark	whether,required
R780	T1284	T1276	conj	required,mode
R781	T1285	T1286	det	the,sequences
R782	T1286	T1284	nsubjpass	sequences,required
R783	T1287	T1286	compound	thymine,sequences
R784	T1288	T1284	auxpass	are,required
R785	T1289	T1284	prep	for,required
R786	T1290	T1291	amod	continuous,activation
R787	T1291	T1289	pobj	activation,for
R788	T1292	T1291	prep	of,activation
R789	T1293	T1294	compound	Mcm,helicase
R790	T1294	T1292	pobj	helicase,of
R791	T1295	T1284	prep	during,required
R792	T1296	T1297	det	the,process
R793	T1297	T1295	pobj	process,during
R794	T1298	T1297	amod	unwinding,process
R795	T1299	T1266	punct	.,addressed
R796	T1301	T1302	det	The,results
R797	T1302	T1303	nsubj	results,indicate
R798	T1304	T1305	mark	that,binds
R799	T1305	T1303	advcl	binds,indicate
R800	T1306	T1307	amod	mammalian,Mcm4
R801	T1307	T1305	nsubj	Mcm4,binds
R802	T1308	T1307	punct	/,Mcm4
R803	T1309	T1307	nummod	6,Mcm4
R804	T1310	T1307	punct	/,Mcm4
R805	T1311	T1307	nummod	7,Mcm4
R806	T1312	T1305	advmod	primarily,binds
R807	T1313	T1305	prep	to,binds
R808	T1314	T1315	amod	single,stranded
R809	T1315	T1317	amod	stranded,region
R810	T1316	T1315	punct	-,stranded
R811	T1317	T1313	pobj	region,to
R812	T1318	T1317	compound	DNA,region
R813	T1319	T1305	punct	", ",binds
R814	T1320	T1305	cc	and,binds
R815	T1321	T1322	mark	that,related
R817	T1323	T1324	det	the,extent
R818	T1324	T1322	nsubjpass	extent,related
R819	T1325	T1324	prep	of,extent
R820	T1326	T1327	compound	helicase,activation
R821	T1327	T1325	pobj	activation,of
R822	T1328	T1322	auxpass	is,related
R823	T1329	T1322	prep	to,related
R824	T1330	T1331	det	the,content
R825	T1331	T1329	pobj	content,to
R826	T1332	T1331	compound	thymine,content
R827	T1333	T1331	prep	of,content
R828	T1334	T1335	det	the,segment
R829	T1335	T1333	pobj	segment,of
R830	T1336	T1337	amod	single,stranded
R831	T1337	T1335	amod	stranded,segment
R832	T1338	T1337	punct	-,stranded
R833	T1339	T1303	punct	.,indicate
R834	T1341	T1342	advmod	Unexpectedly,is
R835	T1343	T1342	punct	", ",is
R836	T1344	T1345	nmod	Mcm4,helicase
R837	T1345	T1342	nsubj	helicase,is
R838	T1346	T1344	punct	/,Mcm4
R839	T1347	T1344	nummod	6,Mcm4
R840	T1348	T1344	punct	/,Mcm4
R841	T1349	T1344	nummod	7,Mcm4
R842	T1350	T1342	neg	not,is
R843	T1351	T1342	acomp	capable,is
R844	T1352	T1351	prep	of,capable
R845	T1353	T1354	advmod	efficiently,unwinding
R846	T1354	T1352	pcomp	unwinding,of
R847	T1355	T1356	det	the,segment
R848	T1356	T1354	dobj	segment,unwinding
R849	T1357	T1358	npadvmod	GC,rich
R850	T1358	T1356	amod	rich,segment
R851	T1359	T1358	punct	-,rich
R852	T1360	T1356	compound	duplex,segment
R853	T1361	T1342	punct	", ",is
R854	T1362	T1342	advcl	suggesting,is
R855	T1363	T1364	mark	that,required
R856	T1364	T1362	ccomp	required,suggesting
R857	T1365	T1366	det	some,mechanism
R858	T1366	T1364	nsubjpass	mechanism,required
R859	T1367	T1366	amod	other,mechanism
R860	T1368	T1364	aux	may,required
R861	T1369	T1364	auxpass	be,required
R862	T1370	T1364	prep	for,required
R863	T1371	T1370	pobj	completion,for
R864	T1372	T1371	prep	of,completion
R865	T1373	T1372	pobj	replication,of
R866	T1374	T1373	prep	of,replication
R867	T1375	T1376	det	the,genome
R868	T1376	T1374	pobj	genome,of
R869	T1377	T1376	amod	entire,genome
R870	T1378	T1342	punct	.,is
R872	T1450	T1451	amod	Labeled,rNTPs
R873	T1451	T1456	nsubjpass	rNTPs,purchased
R874	T1452	T1450	cc	and,Labeled
R875	T1453	T1450	conj	unlabeled,Labeled
R876	T1454	T1451	compound	dNTPs,rNTPs
R877	T1455	T1451	punct	/,rNTPs
R878	T1457	T1451	cc	and,rNTPs
R879	T1458	T1451	conj	Sequenase,rNTPs
R880	T1459	T1456	auxpass	were,purchased
R881	T1460	T1456	prep	from,purchased
R882	T1461	T1462	compound	Amersham,Pharmacia
R883	T1462	T1460	pobj	Pharmacia,from
R884	T1463	T1456	punct	.,purchased
R885	T1465	T1466	nmod	M13mp18,DNA
R886	T1466	T1473	nsubj	DNA,were
R887	T1467	T1465	cc	and,M13mp18
R888	T1468	T1465	conj	M13mp19,M13mp18
R889	T1469	T1470	amod	single,stranded
R890	T1470	T1466	amod	stranded,DNA
R891	T1471	T1470	punct	-,stranded
R892	T1472	T1466	amod	circular,DNA
R893	T1474	T1466	punct	", ",DNA
R894	T1475	T1476	compound	T4,kinase
R895	T1476	T1466	conj	kinase,DNA
R896	T1477	T1476	compound	polynucleotide,kinase
R897	T1478	T1476	punct	", ",kinase
R898	T1479	T1476	conj	DNase,kinase
R899	T1480	T1479	nummod	I,DNase
R900	T1481	T1479	cc	and,DNase
R901	T1482	T1483	det	the,fragment
R902	T1483	T1479	conj	fragment,DNase
R903	T1484	T1483	compound	Klenow,fragment
R904	T1485	T1473	prep	from,were
R905	T1486	T1485	pobj	TAKARA,from
R906	T1487	T1473	punct	", ",were
R907	T1488	T1489	compound	Nuclease,P1
R908	T1489	T1490	nsubj	P1,was
R909	T1490	T1473	conj	was,were
R910	T1491	T1490	prep	from,was
R911	T1492	T1491	pobj	Roche,from
R912	T1493	T1490	punct	", ",was
R913	T1494	T1490	cc	and,was
R914	T1495	T1496	amod	anti-FLAG,agarose
R915	T1496	T1503	nsubj	agarose,were
R916	T1497	T1496	nmod	M2,agarose
R917	T1498	T1499	nmod	Ab,antibody
R918	T1499	T1496	nmod	antibody,agarose
R919	T1500	T1499	punct	(,antibody
R920	T1501	T1496	punct	),agarose
R921	T1502	T1496	punct	-,agarose
R922	T1503	T1490	conj	were,was
R923	T1504	T1496	cc	and,agarose
R924	T1505	T1506	compound	FLAG,peptide
R925	T1506	T1496	conj	peptide,agarose
R926	T1507	T1503	prep	from,were
R927	T1508	T1507	pobj	Sigma,from
R928	T1509	T1503	punct	.,were
R929	T1511	T1512	compound	SV40,antigen
R930	T1512	T1515	nsubjpass	antigen,purified
R931	T1513	T1512	compound	T,antigen
R932	T1514	T1512	punct	-,antigen
R933	T1516	T1515	auxpass	was,purified
R934	T1517	T1515	prep	from,purified
R935	T1518	T1519	npadvmod	baculovirus,infected
R936	T1519	T1521	amod	infected,cells
R937	T1520	T1519	punct	-,infected
R938	T1521	T1517	pobj	cells,from
R939	T1522	T1521	compound	insect,cells
R940	T1523	T1524	mark	as,reported
R941	T1524	T1515	advcl	reported,purified
R942	T1525	T1526	punct	(,22
R943	T1526	T1524	parataxis	22,reported
R944	T1527	T1526	punct	),22
R945	T1528	T1515	punct	.,purified
R946	T1530	T1531	compound	PriA,helicase
R947	T1531	T1532	compound	helicase,protein
R948	T1532	T1533	nsubjpass	protein,provided
R949	T1534	T1533	auxpass	was,provided
R950	T1535	T1533	agent	by,provided
R951	T1536	T1537	compound	Dr,Tanaka
R952	T1537	T1535	pobj	Tanaka,by
R953	T1538	T1537	compound	Taku,Tanaka
R954	T1539	T1540	punct	(,23
R955	T1540	T1533	parataxis	23,provided
R956	T1541	T1540	punct	),23
R957	T1542	T1533	punct	.,provided
R958	T1544	T1545	nsubjpass	Oligonucleotides,purchased
R959	T1546	T1545	auxpass	were,purchased
R960	T1547	T1545	prep	from,purchased
R961	T1548	T1549	nmod	Hokkaido,Ltd
R962	T1549	T1547	pobj	Ltd,from
R963	T1550	T1549	nmod	system,Ltd
R964	T1551	T1549	nmod	science,Ltd
R965	T1552	T1549	nmod	Co.,Ltd
R966	T1553	T1549	punct	", ",Ltd
R967	T1554	T1555	punct	(,Hokkaido
R968	T1555	T1549	parataxis	Hokkaido,Ltd
R969	T1556	T1555	punct	", ",Hokkaido
R970	T1557	T1555	npadvmod	Japan,Hokkaido
R971	T1558	T1555	punct	),Hokkaido
R972	T1559	T1545	punct	", ",purchased
R973	T1560	T1545	cc	and,purchased
R974	T1561	T1562	auxpass	were,purified
R975	T1562	T1545	conj	purified,purchased
R976	T1563	T1562	prep	on,purified
R977	T1564	T1565	compound	polyacrylamide,gel
R978	T1565	T1563	pobj	gel,on
R979	T1566	T1562	punct	", ",purified
R980	T1567	T1562	advcl	followed,purified
R981	T1568	T1567	agent	by,followed
R982	T1569	T1568	pobj	purification,by
R983	T1570	T1569	prep	by,purification
R984	T1571	T1572	compound	Sep,Plus
R985	T1572	T1575	compound	Plus,cartridge
R986	T1573	T1572	punct	-,Plus
R987	T1574	T1572	compound	Pak,Plus
R988	T1575	T1577	compound	cartridge,column
R989	T1576	T1575	compound	C18,cartridge
R990	T1577	T1570	pobj	column,by
R991	T1578	T1579	punct	(,Waters
R992	T1579	T1577	parataxis	Waters,column
R993	T1580	T1579	punct	),Waters
R994	T1581	T1545	punct	.,purchased
R995	T1668	T1667	cc	and,Expression
R996	T1669	T1667	conj	purification,Expression
R997	T1670	T1667	prep	of,Expression
R998	T1671	T1672	nmod	mouse,complex
R1000	T1673	T1672	nmod	Mcm4,complex
R1001	T1674	T1673	punct	/,Mcm4
R1002	T1675	T1673	nummod	6,Mcm4
R1003	T1676	T1673	punct	/,Mcm4
R1004	T1677	T1673	nummod	7,Mcm4
R1005	T1679	T1680	det	The,baculoviruses
R1006	T1680	T1682	nsubjpass	baculoviruses,described
R1007	T1681	T1680	amod	recombinant,baculoviruses
R1008	T1683	T1680	acl	expressing,baculoviruses
R1009	T1684	T1685	nmod	His6,Mcm6
R1010	T1685	T1689	nmod	Mcm6,proteins
R1011	T1686	T1685	punct	-,Mcm6
R1012	T1687	T1685	nmod	Mcm4,Mcm6
R1013	T1688	T1685	punct	/,Mcm6
R1014	T1689	T1683	dobj	proteins,expressing
R1015	T1690	T1685	cc	or,Mcm6
R1016	T1691	T1692	compound	Mcm7,FLAG
R1017	T1692	T1685	conj	FLAG,Mcm6
R1018	T1693	T1692	punct	-,FLAG
R1019	T1694	T1682	auxpass	were,described
R1020	T1695	T1682	advmod	previously,described
R1021	T1696	T1697	punct	(,11
R1022	T1697	T1682	parataxis	11,described
R1023	T1698	T1697	punct	),11
R1024	T1699	T1682	punct	.,described
R1025	T1701	T1702	nmod	Sf9,cells
R1026	T1702	T1707	nsubjpass	cells,cultured
R1027	T1703	T1701	cc	and,Sf9
R1028	T1704	T1705	amod	High,five
R1029	T1705	T1701	conj	five,Sf9
R1030	T1706	T1702	compound	insect,cells
R1031	T1708	T1707	auxpass	were,cultured
R1032	T1709	T1707	prep	at,cultured
R1033	T1710	T1711	nummod	27,°C
R1034	T1711	T1709	pobj	°C,at
R1035	T1712	T1707	prep	in,cultured
R1036	T1713	T1714	nmod	Sf,SFM
R1037	T1714	T1712	pobj	SFM,in
R1038	T1715	T1713	punct	-,Sf
R1039	T1716	T1713	nummod	900,Sf
R1040	T1717	T1714	nummod	II,SFM
R1041	T1718	T1719	punct	(,Inc.
R1042	T1719	T1714	parataxis	Inc.,SFM
R1043	T1720	T1719	nmod	Life,Inc.
R1044	T1721	T1719	nmod	Technologies,Inc.
R1045	T1722	T1719	punct	", ",Inc.
R1046	T1723	T1719	punct	),Inc.
R1047	T1724	T1714	cc	and,SFM
R1048	T1725	T1726	nmod	EX,CELL
R1049	T1726	T1728	nmod	CELL,medium
R1050	T1727	T1726	punct	-,CELL
R1051	T1728	T1714	conj	medium,SFM
R1052	T1729	T1726	nummod	405,CELL
R1053	T1730	T1731	punct	(,Biosciences
R1054	T1731	T1728	parataxis	Biosciences,medium
R1055	T1732	T1731	compound	JRH,Biosciences
R1056	T1733	T1731	punct	),Biosciences
R1057	T1734	T1707	punct	", ",cultured
R1058	T1735	T1707	advmod	respectively,cultured
R1059	T1736	T1707	punct	.,cultured
R1060	T1738	T1739	prep	For,coinfected
R1061	T1740	T1738	pobj	expression,For
R1062	T1741	T1740	prep	of,expression
R1063	T1742	T1743	det	the,proteins
R1065	T1744	T1743	nmod	Mcm4,proteins
R1066	T1745	T1744	punct	/,Mcm4
R1067	T1746	T1744	nummod	6,Mcm4
R1068	T1747	T1744	punct	/,Mcm4
R1069	T1748	T1744	nummod	7,Mcm4
R1070	T1749	T1739	punct	", ",coinfected
R1071	T1750	T1751	amod	High,five
R1072	T1751	T1752	nummod	five,cells
R1073	T1752	T1739	nsubjpass	cells,coinfected
R1074	T1753	T1739	auxpass	were,coinfected
R1075	T1754	T1739	prep	with,coinfected
R1076	T1755	T1756	amod	recombinant,baculoviruses
R1077	T1756	T1754	pobj	baculoviruses,with
R1078	T1757	T1756	acl	expressing,baculoviruses
R1079	T1758	T1759	det	the,proteins
R1081	T1760	T1761	compound	His6,Mcm6
R1082	T1761	T1759	compound	Mcm6,proteins
R1083	T1762	T1761	punct	-,Mcm6
R1084	T1763	T1761	compound	Mcm4,Mcm6
R1085	T1764	T1761	punct	/,Mcm6
R1086	T1765	T1756	cc	and,baculoviruses
R1087	T1766	T1756	conj	those,baculoviruses
R1088	T1767	T1766	acl	expressing,those
R1089	T1768	T1769	det	the,FLAG
R1090	T1769	T1767	dobj	FLAG,expressing
R1091	T1770	T1769	compound	Mcm7,FLAG
R1092	T1771	T1769	punct	-,FLAG
R1093	T1772	T1739	punct	", ",coinfected
R1094	T1773	T1739	cc	and,coinfected
R1095	T1774	T1775	auxpass	were,collected
R1096	T1775	T1739	conj	collected,coinfected
R1097	T1776	T1775	prep	at,collected
R1098	T1777	T1778	nummod	48,h
R1099	T1778	T1776	pobj	h,at
R1100	T1779	T1778	advmod	post-infection,h
R1101	T1780	T1739	punct	.,coinfected
R1102	T1782	T1783	det	The,complexes
R1103	T1783	T1790	nsubjpass	complexes,purified
R1104	T1784	T1783	amod	recombinant,complexes
R1105	T1785	T1783	nmod	Mcm4,complexes
R1106	T1786	T1785	punct	/,Mcm4
R1107	T1787	T1785	nummod	6,Mcm4
R1108	T1788	T1785	punct	/,Mcm4
R1109	T1789	T1785	nummod	7,Mcm4
R1110	T1791	T1790	auxpass	were,purified
R1111	T1792	T1793	mark	as,described
R1112	T1793	T1790	advcl	described,purified
R1113	T1794	T1793	advmod	previously,described
R1114	T1795	T1796	punct	(,12
R1115	T1796	T1793	parataxis	12,described
R1116	T1797	T1796	punct	),12
R1117	T1798	T1790	punct	.,purified
R1124	T1980	T1981	compound	DNA,substrates
R1125	T1983	T1984	det	The,sequences
R1126	T1984	T1985	nsubjpass	sequences,listed
R1127	T1986	T1984	prep	for,sequences
R1128	T1987	T1988	predet	all,oligonucleotides
R1129	T1988	T1986	pobj	oligonucleotides,for
R1130	T1989	T1988	det	the,oligonucleotides
R1131	T1990	T1988	acl	used,oligonucleotides
R1132	T1991	T1990	prep	for,used
R1133	T1992	T1991	pobj	constructions,for
R1134	T1993	T1992	prep	of,constructions
R1135	T1994	T1995	compound	DNA,substrates
R1136	T1995	T1993	pobj	substrates,of
R1137	T1996	T1985	auxpass	are,listed
R1138	T1997	T1985	prep	in,listed
R1139	T1998	T1999	nmod	Tables,1
R1140	T1999	T1997	pobj	1,in
R1141	T2000	T1999	cc	and,1
R1142	T2001	T1999	conj	2,1
R1143	T2002	T1985	punct	.,listed
R1144	T2004	T2005	det	The,substrates
R1145	T2005	T2008	nsubjpass	substrates,constructed
R1146	T2006	T2005	amod	partial,substrates
R1147	T2007	T2005	compound	heteroduplex,substrates
R1148	T2009	T2008	auxpass	were,constructed
R1149	T2010	T2008	prep	by,constructed
R1150	T2011	T2010	pcomp	annealing,by
R1151	T2012	T2013	amod	labeled,oligonucleotides
R1152	T2013	T2011	dobj	oligonucleotides,annealing
R1153	T2014	T2011	prep	with,annealing
R1154	T2015	T2016	nmod	M13mp18,DNA
R1155	T2016	T2014	pobj	DNA,with
R1156	T2017	T2018	amod	single,stranded
R1157	T2018	T2016	amod	stranded,DNA
R1158	T2019	T2018	punct	-,stranded
R1159	T2020	T2016	amod	circular,DNA
R1160	T2021	T2016	cc	or,DNA
R1161	T2022	T2023	poss	its,derivative
R1162	T2023	T2016	conj	derivative,DNA
R1163	T2024	T2008	punct	.,constructed
R1164	T2026	T2027	det	The,substrates
R1165	T2027	T2029	nsubjpass	substrates,purified
R1166	T2028	T2027	amod	labeled,substrates
R1167	T2030	T2029	auxpass	were,purified
R1168	T2031	T2029	agent	by,purified
R1169	T2032	T2033	compound	Sepharose,CL4B
R1170	T2033	T2034	compound	CL4B,chromatography
R1171	T2034	T2031	pobj	chromatography,by
R1172	T2035	T2034	compound	column,chromatography
R1173	T2036	T2037	punct	(,Biotech
R1174	T2037	T2029	parataxis	Biotech,purified
R1175	T2038	T2037	compound	Amersham,Biotech
R1176	T2039	T2037	compound	Pharmacia,Biotech
R1177	T2040	T2037	punct	),Biotech
R1178	T2041	T2029	punct	.,purified
R1179	T2043	T2044	det	The,substrates
R1180	T2044	T2047	nsubjpass	substrates,assembled
R1181	T2045	T2046	compound	DNA,bubble
R1182	T2046	T2044	compound	bubble,substrates
R1183	T2048	T2047	auxpass	were,assembled
R1184	T2049	T2047	prep	from,assembled
R1185	T2050	T2051	nummod	two,oligonucleotides
R1186	T2051	T2049	pobj	oligonucleotides,from
R1187	T2052	T2053	advmod	partially,complementary
R1188	T2053	T2051	amod	complementary,oligonucleotides
R1189	T2054	T2051	prep	with,oligonucleotides
R1190	T2055	T2056	amod	top,sequences
R1191	T2056	T2054	pobj	sequences,with
R1192	T2057	T2055	cc	and,top
R1193	T2058	T2055	conj	bottom,top
R1194	T2059	T2056	compound	strand,sequences
R1195	T2060	T2061	punct	(,Table
R1196	T2061	T2051	parataxis	Table,oligonucleotides
R1197	T2062	T2061	nummod	1,Table
R1198	T2063	T2061	punct	),Table
R1199	T2064	T2047	punct	.,assembled
R1200	T2066	T2067	npadvmod	T,tailed
R1201	T2067	T2069	amod	tailed,substrates
R1202	T2068	T2067	punct	-,tailed
R1203	T2069	T2073	nsubjpass	substrates,prepared
R1204	T2070	T2071	compound	Y,fork
R1205	T2071	T2069	compound	fork,substrates
R1206	T2072	T2071	punct	-,fork
R1207	T2074	T2073	auxpass	were,prepared
R1208	T2075	T2073	prep	by,prepared
R1209	T2076	T2075	pcomp	annealing,by
R1210	T2077	T2078	nummod	two,oligonucleotides
R1211	T2078	T2076	dobj	oligonucleotides,annealing
R1212	T2079	T2080	punct	(,series
R1213	T2080	T2078	parataxis	series,oligonucleotides
R1214	T2081	T2080	nmod	dT30,series
R1215	T2082	T2081	cc	and,dT30
R1216	T2083	T2081	conj	dT60,dT30
R1217	T2084	T2080	punct	),series
R1218	T2085	T2073	punct	.,prepared
R1219	T2087	T2088	aux	To,construct
R1220	T2088	T2089	advcl	construct,annealed
R1221	T2090	T2091	det	the,fork
R1222	T2091	T2088	dobj	fork,construct
R1223	T2092	T2091	amod	arrested,fork
R1224	T2093	T2091	cc	and,fork
R1225	T2094	T2095	nummod	3,substrates
R1226	T2095	T2091	conj	substrates,fork
R1227	T2096	T2094	punct	′,3
R1228	T2097	T2094	punct	-,3
R1229	T2098	T2094	cc	or,3
R1230	T2099	T2094	conj	5,3
R1231	T2100	T2099	punct	′,5
R1232	T2101	T2095	punct	-,substrates
R1233	T2102	T2095	compound	extension,substrates
R1234	T2103	T2089	punct	", ",annealed
R1235	T2104	T2105	amod	various,combinations
R1236	T2105	T2089	nsubjpass	combinations,annealed
R1237	T2106	T2105	prep	of,combinations
R1238	T2107	T2106	pobj	oligonucleotides,of
R1239	T2108	T2107	acl	shown,oligonucleotides
R1240	T2109	T2108	prep	in,shown
R1241	T2110	T2109	pobj	Table,in
R1242	T2111	T2110	nummod	1,Table
R1243	T2112	T2089	auxpass	were,annealed
R1244	T2113	T2089	punct	.,annealed
R1245	T2115	T2116	nmod	Bub,B2
R1246	T2116	T2121	nsubjpass	B2,constructed
R1247	T2117	T2116	punct	-,B2
R1248	T2118	T2116	nummod	82,B2
R1249	T2119	T2116	punct	/,B2
R1250	T2120	T2116	compound	lamin,B2
R1251	T2122	T2116	acl	containing,B2
R1252	T2123	T2124	det	the,strand
R1253	T2124	T2122	dobj	strand,containing
R1254	T2125	T2126	npadvmod	T,rich
R1255	T2126	T2124	amod	rich,strand
R1256	T2127	T2126	punct	-,rich
R1257	T2128	T2124	prep	of,strand
R1258	T2129	T2130	det	the,region
R1259	T2130	T2128	pobj	region,of
R1260	T2131	T2132	compound	lamin,B2
R1261	T2132	T2130	compound	B2,region
R1262	T2133	T2130	compound	origin,region
R1263	T2134	T2121	auxpass	was,constructed
R1264	T2135	T2136	mark	as,described
R1265	T2136	T2121	advcl	described,constructed
R1266	T2137	T2136	advmod	previously,described
R1267	T2138	T2139	punct	(,13
R1268	T2139	T2136	parataxis	13,described
R1269	T2140	T2139	punct	),13
R1270	T2141	T2121	punct	.,constructed
R1271	T2143	T2144	det	The,substrate
R1272	T2144	T2146	nsubj	substrate,contains
R1273	T2145	T2144	compound	bubble,substrate
R1274	T2147	T2144	punct	", ",substrate
R1275	T2148	T2149	compound	c,myc
R1276	T2149	T2151	compound	myc,DUE
R1277	T2150	T2149	punct	-,myc
R1278	T2151	T2144	appos	DUE,substrate
R1279	T2152	T2151	punct	/,DUE
R1280	T2153	T2151	cc	or,DUE
R1281	T2154	T2155	compound	c,myc
R1282	T2155	T2157	compound	myc,C
R1283	T2156	T2155	punct	-,myc
R1284	T2157	T2151	conj	C,DUE
R1285	T2158	T2157	punct	/,C
R1286	T2159	T2157	compound	DUE,C
R1287	T2160	T2157	punct	-,C
R1288	T2161	T2146	punct	", ",contains
R1289	T2162	T2146	dobj	sequences,contains
R1290	T2163	T2164	punct	(,K01908
R1291	T2164	T2162	parataxis	K01908,sequences
R1292	T2165	T2166	compound	GenBank,number
R1293	T2166	T2164	compound	number,K01908
R1294	T2167	T2166	compound	accession,number
R1295	T2168	T2164	punct	),K01908
R1296	T2169	T2162	prep	from,sequences
R1297	T2170	T2171	nmod	nucleotides,853
R1298	T2171	T2169	pobj	853,from
R1299	T2172	T2171	nummod	722,853
R1300	T2173	T2171	nmod	to,853
R1301	T2174	T2162	cc	or,sequences
R1302	T2175	T2162	conj	those,sequences
R1303	T2176	T2175	prep	from,those
R1304	T2177	T2176	pobj	828,from
R1305	T2178	T2176	prep	to,from
R1306	T2179	T2178	pobj	747,to
R1307	T2180	T2181	punct	(,strand
R1308	T2181	T2175	parataxis	strand,those
R1309	T2182	T2181	amod	complementary,strand
R1310	T2183	T2181	punct	),strand
R1311	T2184	T2146	punct	", ",contains
R1312	T2185	T2146	advmod	respectively,contains
R1313	T2186	T2146	punct	", ",contains
R1314	T2187	T2146	prep	in,contains
R1315	T2188	T2189	det	the,region
R1316	T2189	T2187	pobj	region,in
R1317	T2190	T2189	amod	central,region
R1318	T2191	T2189	amod	melted,region
R1319	T2192	T2146	punct	.,contains
R1320	T2194	T2195	prep	For,annealed
R1321	T2196	T2197	det	each,substrate
R1322	T2197	T2194	pobj	substrate,For
R1323	T2198	T2195	punct	", ",annealed
R1324	T2199	T2200	amod	labeled,oligonucleotide
R1325	T2200	T2195	nsubjpass	oligonucleotide,annealed
R1326	T2201	T2202	punct	(,pmol
R1327	T2202	T2200	parataxis	pmol,oligonucleotide
R1328	T2203	T2202	nummod	3,pmol
R1329	T2204	T2202	punct	),pmol
R1330	T2205	T2195	auxpass	was,annealed
R1331	T2206	T2195	prep	with,annealed
R1332	T2207	T2208	det	the,oligonucleotide
R1333	T2208	T2206	pobj	oligonucleotide,with
R1334	T2209	T2208	amod	unlabeled,oligonucleotide
R1335	T2210	T2211	punct	(,pmol
R1336	T2211	T2208	parataxis	pmol,oligonucleotide
R1337	T2212	T2211	nummod	6,pmol
R1338	T2213	T2211	punct	),pmol
R1339	T2214	T2195	prep	in,annealed
R1340	T2215	T2216	det	a,mixture
R1341	T2216	T2214	pobj	mixture,in
R1342	T2217	T2216	compound	reaction,mixture
R1343	T2218	T2219	punct	(,μl
R1344	T2219	T2216	parataxis	μl,mixture
R1345	T2220	T2219	nummod	50,μl
R1346	T2221	T2219	punct	),μl
R1347	T2222	T2216	acl	containing,mixture
R1348	T2223	T2224	nummod	20,mM
R1349	T2224	T2225	compound	mM,HCl
R1350	T2225	T2222	dobj	HCl,containing
R1351	T2226	T2225	compound	Tris,HCl
R1352	T2227	T2225	punct	–,HCl
R1353	T2228	T2229	punct	(,pH
R1354	T2229	T2225	parataxis	pH,HCl
R1355	T2230	T2229	nummod	7.5,pH
R1356	T2231	T2229	punct	),pH
R1357	T2232	T2225	punct	", ",HCl
R1358	T2233	T2234	nummod	10,mM
R1359	T2234	T2235	compound	mM,MgCl2
R1360	T2235	T2225	conj	MgCl2,HCl
R1361	T2236	T2235	cc	and,MgCl2
R1362	T2237	T2238	nummod	25,mM
R1363	T2238	T2239	compound	mM,NaCl
R1364	T2239	T2235	conj	NaCl,MgCl2
R1365	T2240	T2216	punct	", ",mixture
R1366	T2241	T2242	dep	which,heated
R1367	T2242	T2216	relcl	heated,mixture
R1368	T2243	T2242	auxpass	were,heated
R1369	T2244	T2242	prep	to,heated
R1370	T2245	T2246	nummod	95,°C
R1371	T2246	T2244	pobj	°C,to
R1372	T2247	T2242	punct	", ",heated
R1373	T2248	T2242	conj	kept,heated
R1374	T2249	T2248	prep	at,kept
R1375	T2250	T2251	nummod	67,°C
R1376	T2251	T2249	pobj	°C,at
R1377	T2252	T2248	prep	for,kept
R1378	T2253	T2254	nummod	1,h
R1379	T2254	T2252	pobj	h,for
R1380	T2255	T2248	punct	", ",kept
R1381	T2256	T2248	cc	and,kept
R1382	T2257	T2258	advmod	then,allowed
R1383	T2258	T2248	conj	allowed,kept
R1384	T2259	T2260	aux	to,cool
R1385	T2260	T2258	xcomp	cool,allowed
R1386	T2261	T2260	advmod	slowly,cool
R1387	T2262	T2260	prt	down,cool
R1388	T2263	T2260	prep	to,cool
R1389	T2264	T2265	nummod	37,°C
R1390	T2265	T2263	pobj	°C,to
R1391	T2266	T2195	punct	.,annealed
R1392	T2268	T2269	det	The,substrates
R1393	T2269	T2271	nsubjpass	substrates,purified
R1394	T2270	T2269	amod	assembled,substrates
R1395	T2272	T2271	auxpass	were,purified
R1396	T2273	T2271	prep	from,purified
R1397	T2274	T2275	compound	polyacrylamide,gel
R1398	T2275	T2273	pobj	gel,from
R1399	T2276	T2271	prep	by,purified
R1400	T2277	T2276	pobj	elution,by
R1401	T2278	T2277	prep	into,elution
R1402	T2279	T2280	compound	TE,buffer
R1403	T2280	T2278	pobj	buffer,into
R1404	T2281	T2282	punct	(,24
R1405	T2282	T2271	parataxis	24,purified
R1406	T2283	T2282	punct	),24
R1407	T2284	T2271	punct	.,purified
R1408	T2286	T2287	det	The,substrates
R1409	T2287	T2290	nsubjpass	substrates,prepared
R1410	T2288	T2287	amod	partial,substrates
R1411	T2289	T2287	compound	heteroduplex,substrates
R1412	T2291	T2287	prep	on,substrates
R1413	T2292	T2293	det	a,DNA
R1414	T2293	T2291	pobj	DNA,on
R1415	T2294	T2295	amod	single,stranded
R1416	T2295	T2293	amod	stranded,DNA
R1417	T2296	T2295	punct	-,stranded
R1418	T2297	T2293	amod	circular,DNA
R1419	T2298	T2293	prep	with,DNA
R1420	T2299	T2300	amod	varied,lengths
R1421	T2300	T2298	pobj	lengths,with
R1422	T2301	T2300	prep	of,lengths
R1423	T2302	T2303	compound	duplex,regions
R1424	T2303	T2301	pobj	regions,of
R1425	T2304	T2290	auxpass	were,prepared
R1426	T2305	T2290	prep	by,prepared
R1427	T2306	T2305	pcomp	extending,by
R1428	T2307	T2308	compound	DNA,chains
R1429	T2308	T2306	dobj	chains,extending
R1430	T2309	T2306	prep	from,extending
R1431	T2310	T2311	det	the,end
R1432	T2311	T2309	pobj	end,from
R1433	T2312	T2311	nummod	3,end
R1434	T2313	T2312	punct	′,3
R1435	T2314	T2311	prep	of,end
R1436	T2315	T2316	det	the,37mer
R1437	T2316	T2314	pobj	37mer,of
R1438	T2317	T2316	compound	dT40,37mer
R1439	T2318	T2316	punct	-,37mer
R1440	T2319	T2316	punct	(,37mer
R1441	T2320	T2321	compound	37mer,region
R1442	T2321	T2316	appos	region,37mer
R1443	T2322	T2321	acl	hybridizing,region
R1444	T2323	T2322	prep	at,hybridizing
R1445	T2324	T2325	nmod	positions,6289
R1446	T2325	T2323	pobj	6289,at
R1447	T2326	T2327	punct	–,6326
R1448	T2327	T2325	prep	6326,6289
R1449	T2328	T2325	prep	of,6289
R1450	T2329	T2330	compound	M13mp18,vector
R1451	T2330	T2328	pobj	vector,of
R1452	T2331	T2316	punct	),37mer
R1453	T2332	T2316	acl	annealed,37mer
R1454	T2333	T2332	prep	to,annealed
R1455	T2334	T2335	det	the,DNA
R1456	T2335	T2333	pobj	DNA,to
R1457	T2336	T2337	amod	single,stranded
R1458	T2337	T2335	amod	stranded,DNA
R1459	T2338	T2337	punct	-,stranded
R1460	T2339	T2335	amod	circular,DNA
R1461	T2340	T2290	punct	.,prepared
R1462	T2342	T2343	compound	DNA,chains
R1463	T2343	T2344	nsubjpass	chains,elongated
R1464	T2345	T2344	auxpass	were,elongated
R1465	T2346	T2344	prep	with,elongated
R1466	T2347	T2346	pobj	Sequenase,with
R1467	T2348	T2344	prep	in,elongated
R1468	T2349	T2350	det	the,presence
R1469	T2350	T2348	pobj	presence,in
R1470	T2351	T2350	prep	of,presence
R1471	T2352	T2353	punct	[,dGTP
R1472	T2353	T2351	pobj	dGTP,of
R1473	T2354	T2355	nmod	α,32P
R1474	T2355	T2353	nmod	32P,dGTP
R1475	T2356	T2355	punct	-,32P
R1476	T2357	T2353	punct	],dGTP
R1477	T2358	T2353	punct	", ",dGTP
R1478	T2359	T2353	acl	followed,dGTP
R1479	T2360	T2359	agent	by,followed
R1480	T2361	T2360	pobj	extension,by
R1481	T2362	T2359	prep	after,followed
R1482	T2363	T2362	pobj	addition,after
R1483	T2364	T2363	prep	of,addition
R1484	T2365	T2364	pobj	ddGTP,of
R1485	T2366	T2365	cc	and,ddGTP
R1486	T2367	T2368	det	all,dNTPs
R1487	T2368	T2365	conj	dNTPs,ddGTP
R1488	T2369	T2368	nummod	four,dNTPs
R1489	T2370	T2344	punct	", ",elongated
R1490	T2371	T2344	advcl	resulting,elongated
R1491	T2372	T2371	prep	in,resulting
R1492	T2373	T2372	pobj	substrates,in
R1493	T2374	T2373	acl	containing,substrates
R1494	T2375	T2376	amod	labeled,regions
R1495	T2376	T2374	dobj	regions,containing
R1496	T2377	T2376	compound	duplex,regions
R1497	T2378	T2376	prep	of,regions
R1498	T2379	T2380	amod	varied,lengths
R1499	T2380	T2378	pobj	lengths,of
R1500	T2381	T2382	punct	(,13
R1501	T2382	T2344	parataxis	13,elongated
R1502	T2383	T2382	punct	),13
R1503	T2384	T2344	punct	.,elongated
R1504	T2386	T2387	det	The,substrate
R1505	T2387	T2388	nsubjpass	substrate,incubated
R1506	T2389	T2390	punct	(,fmol
R1507	T2390	T2387	parataxis	fmol,substrate
R1508	T2391	T2390	nummod	5,fmol
R1509	T2392	T2390	punct	),fmol
R1510	T2393	T2388	auxpass	was,incubated
R1511	T2394	T2388	advmod	first,incubated
R1512	T2395	T2388	prep	with,incubated
R1513	T2396	T2397	amod	indicated,amount
R1514	T2397	T2395	pobj	amount,with
R1515	T2398	T2397	prep	of,amount
R1516	T2399	T2400	det	the,complex
R1517	T2400	T2398	pobj	complex,of
R1518	T2401	T2400	nmod	Mcm4,complex
R1519	T2402	T2401	punct	/,Mcm4
R1520	T2403	T2401	nummod	6,Mcm4
R1521	T2404	T2401	punct	/,Mcm4
R1522	T2405	T2401	nummod	7,Mcm4
R1523	T2406	T2388	prep	at,incubated
R1524	T2407	T2408	nummod	37,°C
R1525	T2408	T2406	pobj	°C,at
R1526	T2409	T2388	prep	for,incubated
R1527	T2410	T2411	nummod	1,h
R1528	T2411	T2409	pobj	h,for
R1529	T2412	T2388	prep	in,incubated
R1530	T2413	T2414	compound	reaction,mixture
R1531	T2414	T2412	pobj	mixture,in
R1532	T2415	T2416	mark	as,described
R1533	T2416	T2388	advcl	described,incubated
R1534	T2417	T2418	punct	(,11
R1535	T2418	T2416	parataxis	11,described
R1536	T2419	T2418	punct	),11
R1537	T2420	T2388	punct	", ",incubated
R1538	T2421	T2388	cc	and,incubated
R1539	T2422	T2421	advmod	then,and
R1540	T2423	T2424	nsubjpass	reactions,stopped
R1541	T2424	T2388	conj	stopped,incubated
R1542	T2425	T2424	auxpass	were,stopped
R1543	T2426	T2424	agent	by,stopped
R1544	T2427	T2426	pobj	addition,by
R1545	T2428	T2427	prep	of,addition
R1546	T2429	T2428	pobj	EDTA,of
R1547	T2430	T2431	punct	(,mM
R1548	T2431	T2429	parataxis	mM,EDTA
R1549	T2432	T2431	nummod	20,mM
R1550	T2433	T2431	punct	),mM
R1551	T2434	T2429	punct	", ",EDTA
R1552	T2435	T2429	conj	SDS,EDTA
R1553	T2436	T2437	punct	(,%
R1554	T2437	T2435	parataxis	%,SDS
R1555	T2438	T2437	nummod	0.1,%
R1556	T2439	T2437	punct	),%
R1557	T2440	T2435	cc	and,SDS
R1558	T2441	T2442	nummod	2,μg
R1559	T2442	T2443	compound	μg,K
R1560	T2443	T2435	conj	K,SDS
R1561	T2444	T2443	compound	proteinase,K
R1562	T2445	T2427	punct	", ",addition
R1563	T2446	T2427	acl	followed,addition
R1564	T2447	T2446	agent	by,followed
R1565	T2448	T2447	pobj	incubation,by
R1566	T2449	T2448	prep	for,incubation
R1567	T2450	T2451	nummod	20,min
R1568	T2451	T2449	pobj	min,for
R1569	T2452	T2424	punct	.,stopped
R1570	T2454	T2455	nsubjpass	Aliquots,electrophoresed
R1571	T2456	T2455	auxpass	were,electrophoresed
R1572	T2457	T2455	prep	on,electrophoresed
R1573	T2458	T2459	det	a,gel
R1574	T2459	T2457	pobj	gel,on
R1575	T2460	T2461	nummod	6.5,%
R1576	T2461	T2459	compound	%,gel
R1577	T2462	T2459	compound	polyacrylamide,gel
R1578	T2463	T2455	prep	in,electrophoresed
R1579	T2464	T2465	nummod	1,TBE
R1580	T2465	T2463	pobj	TBE,in
R1581	T2466	T2464	punct	×,1
R1582	T2467	T2455	prep	at,electrophoresed
R1583	T2468	T2469	nummod	300,V
R1584	T2469	T2467	pobj	V,at
R1585	T2470	T2455	prep	for,electrophoresed
R1586	T2471	T2472	nummod	2.5,h
R1587	T2472	T2470	pobj	h,for
R1588	T2473	T2455	punct	.,electrophoresed
R1589	T2475	T2476	det	The,DNAs
R1590	T2476	T2481	nsubj	DNAs,were
R1591	T2477	T2478	amod	single,stranded
R1592	T2478	T2476	amod	stranded,DNAs
R1593	T2479	T2478	punct	-,stranded
R1594	T2480	T2476	amod	circular,DNAs
R1595	T2482	T2476	acl	used,DNAs
R1596	T2483	T2481	attr	M13mp18,were
R1597	T2484	T2483	cc	and,M13mp18
R1598	T2485	T2483	conj	M13mp19,M13mp18
R1599	T2486	T2483	punct	+,M13mp18
R1600	T2487	T2483	appos	G,M13mp18
R1601	T2488	T2487	punct	-,G
R1602	T2489	T2487	amod	rich,G
R1603	T2490	T2481	punct	.,were
R1604	T2492	T2493	det	The,latter
R1605	T2493	T2494	nsubjpass	latter,constructed
R1606	T2495	T2494	auxpass	was,constructed
R1607	T2496	T2494	prep	by,constructed
R1608	T2497	T2496	pcomp	inserting,by
R1609	T2498	T2499	det	a,sequence
R1610	T2499	T2497	dobj	sequence,inserting
R1611	T2500	T2499	nmod	G,sequence
R1612	T2501	T2500	punct	-,G
R1613	T2502	T2500	amod	rich,G
R1614	T2503	T2499	compound	repeat,sequence
R1615	T2504	T2499	punct	(,sequence
R1616	T2505	T2499	appos	GGGGCGTGGGC,sequence
R1617	T2506	T2505	punct	),GGGGCGTGGGC
R1618	T2507	T2505	nummod	6,GGGGCGTGGGC
R1619	T2508	T2499	punct	", ",sequence
R1620	T2509	T2499	acl	prepared,sequence
R1621	T2510	T2509	prep	by,prepared
R1622	T2511	T2510	pcomp	annealing,by
R1623	T2512	T2511	prep	of,annealing
R1624	T2513	T2514	nummod	two,oligonucleotides
R1625	T2514	T2512	pobj	oligonucleotides,of
R1626	T2515	T2514	punct	(,oligonucleotides
R1627	T2516	T2514	appos	5,oligonucleotides
R1628	T2517	T2516	punct	′,5
R1629	T2518	T2516	punct	-,5
R1630	T2519	T2520	nmod	GATCC,GGGGCGTGGGC
R1631	T2520	T2516	appos	GGGGCGTGGGC,5
R1632	T2521	T2520	punct	-,GGGGCGTGGGC
R1633	T2522	T2520	punct	[,GGGGCGTGGGC
R1634	T2523	T2520	punct	],GGGGCGTGGGC
R1635	T2524	T2520	nummod	6,GGGGCGTGGGC
R1636	T2525	T2516	punct	-,5
R1637	T2526	T2516	nummod	3,5
R1638	T2527	T2516	punct	′,5
R1639	T2528	T2516	punct	/,5
R1640	T2529	T2516	appos	5,5
R1641	T2530	T2529	punct	′,5
R1642	T2531	T2529	punct	-,5
R1643	T2532	T2533	nmod	GATCC,GCCCACGCCCC
R1644	T2533	T2529	appos	GCCCACGCCCC,5
R1645	T2534	T2533	punct	-,GCCCACGCCCC
R1646	T2535	T2533	punct	[,GCCCACGCCCC
R1647	T2536	T2533	punct	],GCCCACGCCCC
R1648	T2537	T2533	nummod	6,GCCCACGCCCC
R1649	T2538	T2529	punct	-,5
R1650	T2539	T2529	nummod	3,5
R1651	T2540	T2529	punct	′,5
R1652	T2541	T2514	punct	),oligonucleotides
R1653	T2542	T2497	prep	at,inserting
R1654	T2543	T2544	det	the,site
R1655	T2544	T2542	pobj	site,at
R1656	T2545	T2544	compound	BamHI,site
R1657	T2546	T2544	prep	of,site
R1658	T2547	T2546	pobj	M13mp19,of
R1659	T2548	T2494	cc	and,constructed
R1660	T2549	T2550	det	the,sequences
R1661	T2550	T2551	nsubjpass	sequences,confirmed
R1662	T2551	T2494	conj	confirmed,constructed
R1663	T2552	T2550	prep	of,sequences
R1664	T2553	T2554	det	the,insert
R1665	T2554	T2552	pobj	insert,of
R1666	T2555	T2551	auxpass	were,confirmed
R1667	T2556	T2551	punct	.,confirmed
R1668	T2629	T2630	nmod	DNA,helicase
R1669	T2630	T2631	nmod	helicase,assays
R1670	T2632	T2630	cc	and,helicase
R1671	T2633	T2634	compound	gel,shift
R1672	T2634	T2630	conj	shift,helicase
R1673	T2636	T2637	prep	In,incubated
R1674	T2638	T2639	compound	gel,shift
R1675	T2639	T2640	compound	shift,assays
R1676	T2640	T2636	pobj	assays,In
R1677	T2641	T2637	punct	", ",incubated
R1678	T2642	T2643	nmod	Mcm4,proteins
R1679	T2643	T2637	nsubjpass	proteins,incubated
R1680	T2644	T2642	punct	/,Mcm4
R1681	T2645	T2642	nummod	6,Mcm4
R1682	T2646	T2642	punct	/,Mcm4
R1683	T2647	T2642	nummod	7,Mcm4
R1684	T2648	T2637	auxpass	were,incubated
R1685	T2649	T2637	prep	at,incubated
R1686	T2650	T2651	nummod	30,°C
R1687	T2651	T2649	pobj	°C,at
R1688	T2652	T2637	prep	for,incubated
R1689	T2653	T2654	nummod	30,min
R1690	T2654	T2652	pobj	min,for
R1691	T2655	T2637	prep	in,incubated
R1692	T2656	T2657	compound	reaction,mixtures
R1693	T2657	T2655	pobj	mixtures,in
R1694	T2658	T2659	punct	(,μl
R1695	T2659	T2657	parataxis	μl,mixtures
R1696	T2660	T2659	nummod	15,μl
R1697	T2661	T2659	punct	),μl
R1698	T2662	T2657	acl	containing,mixtures
R1699	T2663	T2664	nummod	25,mM
R1700	T2664	T2665	compound	mM,NaOH
R1701	T2665	T2662	dobj	NaOH,containing
R1702	T2666	T2665	compound	HEPES,NaOH
R1703	T2667	T2665	punct	–,NaOH
R1704	T2668	T2669	punct	(,pH
R1705	T2669	T2665	parataxis	pH,NaOH
R1706	T2670	T2669	nummod	7.5,pH
R1707	T2671	T2669	punct	),pH
R1708	T2672	T2665	punct	", ",NaOH
R1709	T2673	T2674	nummod	50,mM
R1710	T2674	T2675	compound	mM,acetate
R1711	T2675	T2665	conj	acetate,NaOH
R1712	T2676	T2675	compound	sodium,acetate
R1713	T2677	T2675	punct	", ",acetate
R1714	T2678	T2679	nummod	10,mM
R1715	T2679	T2680	nmod	mM,CH3COO
R1716	T2680	T2675	conj	CH3COO,acetate
R1717	T2681	T2680	nmod	Mg,CH3COO
R1718	T2682	T2680	punct	(,CH3COO
R1719	T2683	T2680	punct	),CH3COO
R1720	T2684	T2680	nummod	2,CH3COO
R1721	T2685	T2680	punct	", ",CH3COO
R1722	T2686	T2687	nummod	1,mM
R1723	T2687	T2688	compound	mM,DTT
R1724	T2688	T2680	conj	DTT,CH3COO
R1725	T2689	T2688	punct	", ",DTT
R1726	T2690	T2691	nummod	0.25,μg
R1727	T2691	T2692	nmod	μg,BSA
R1728	T2692	T2688	conj	BSA,DTT
R1729	T2693	T2694	punct	/,ml
R1730	T2694	T2691	prep	ml,μg
R1731	T2695	T2692	punct	", ",BSA
R1732	T2696	T2697	nummod	0.5,mM
R1733	T2697	T2698	nmod	mM,S
R1734	T2698	T2692	conj	S,BSA
R1735	T2699	T2698	nmod	ATP,S
R1736	T2700	T2698	punct	-,S
R1737	T2701	T2698	punct	γ,S
R1738	T2702	T2698	punct	-,S
R1739	T2703	T2698	cc	and,S
R1740	T2704	T2705	amod	labeled,substrates
R1741	T2705	T2698	conj	substrates,S
R1742	T2706	T2705	prep	of,substrates
R1743	T2707	T2708	det	the,amount
R1744	T2708	T2706	pobj	amount,of
R1745	T2709	T2708	acl	indicated,amount
R1746	T2710	T2637	punct	.,incubated
R1747	T2712	T2713	prep	After,applied
R1748	T2714	T2712	pobj	addition,After
R1749	T2715	T2714	prep	of,addition
R1750	T2716	T2717	nummod	2,μl
R1751	T2717	T2715	pobj	μl,of
R1752	T2718	T2717	prep	of,μl
R1753	T2719	T2720	nummod	50,%
R1754	T2720	T2721	compound	%,glycerol
R1755	T2721	T2718	pobj	glycerol,of
R1756	T2722	T2713	punct	", ",applied
R1757	T2723	T2724	det	the,mixtures
R1758	T2724	T2713	nsubjpass	mixtures,applied
R1759	T2725	T2724	compound	reaction,mixtures
R1760	T2726	T2713	auxpass	were,applied
R1761	T2727	T2713	advmod	directly,applied
R1762	T2728	T2713	prep	on,applied
R1763	T2729	T2730	det	a,gel
R1764	T2730	T2728	pobj	gel,on
R1765	T2731	T2730	compound	polyacrylamide,gel
R1766	T2732	T2730	acl	containing,gel
R1767	T2733	T2734	nummod	6,mM
R1768	T2734	T2735	compound	mM,acetate
R1769	T2735	T2732	dobj	acetate,containing
R1770	T2736	T2735	compound	magnesium,acetate
R1771	T2737	T2735	cc	and,acetate
R1772	T2738	T2739	nummod	5,%
R1773	T2739	T2740	compound	%,glycerol
R1774	T2740	T2735	conj	glycerol,acetate
R1775	T2741	T2732	prep	in,containing
R1776	T2742	T2743	nummod	0.5,TBE
R1777	T2743	T2741	pobj	TBE,in
R1778	T2744	T2742	punct	×,0.5
R1779	T2745	T2713	punct	", ",applied
R1780	T2746	T2713	cc	and,applied
R1781	T2747	T2748	nsubjpass	electrophoresis,conducted
R1782	T2748	T2713	conj	conducted,applied
R1783	T2749	T2748	auxpass	was,conducted
R1784	T2750	T2748	prep	at,conducted
R1785	T2751	T2752	compound	room,temperature
R1786	T2752	T2750	pobj	temperature,at
R1787	T2753	T2748	punct	.,conducted
R1788	T2755	T2756	prep	For,incubated
R1789	T2757	T2758	compound	DNA,assays
R1790	T2758	T2755	pobj	assays,For
R1791	T2759	T2758	compound	helicase,assays
R1792	T2760	T2756	punct	", ",incubated
R1793	T2761	T2762	det	the,mixtures
R1794	T2762	T2756	nsubjpass	mixtures,incubated
R1795	T2763	T2762	compound	reaction,mixtures
R1796	T2764	T2762	prep	of,mixtures
R1797	T2765	T2766	det	the,assays
R1798	T2766	T2764	pobj	assays,of
R1799	T2767	T2768	compound	gel,shift
R1800	T2768	T2766	compound	shift,assays
R1801	T2769	T2756	punct	", ",incubated
R1802	T2770	T2771	mark	as,described
R1803	T2771	T2756	advcl	described,incubated
R1804	T2772	T2771	advmod	above,described
R1805	T2773	T2756	punct	", ",incubated
R1806	T2774	T2756	auxpass	were,incubated
R1807	T2775	T2756	prep	at,incubated
R1808	T2776	T2777	nummod	30,°C
R1809	T2777	T2775	pobj	°C,at
R1810	T2778	T2756	prep	for,incubated
R1811	T2779	T2780	nummod	15,min
R1812	T2780	T2778	pobj	min,for
R1813	T2781	T2756	punct	", ",incubated
R1814	T2782	T2756	cc	and,incubated
R1815	T2783	T2782	advmod	then,and
R1816	T2784	T2785	det	the,ATP
R1817	T2785	T2786	nsubjpass	ATP,added
R1818	T2786	T2756	conj	added,incubated
R1819	T2787	T2786	auxpass	was,added
R1820	T2788	T2786	prep	at,added
R1821	T2789	T2790	nummod	10,mM
R1822	T2790	T2788	pobj	mM,at
R1823	T2791	T2786	punct	", ",added
R1824	T2792	T2786	advcl	followed,added
R1825	T2793	T2792	agent	by,followed
R1826	T2794	T2793	pobj	incubation,by
R1827	T2795	T2794	prep	at,incubation
R1828	T2796	T2797	nummod	37,°C
R1829	T2797	T2795	pobj	°C,at
R1830	T2798	T2794	prep	for,incubation
R1831	T2799	T2800	amod	additional,min
R1832	T2800	T2798	pobj	min,for
R1833	T2801	T2800	nummod	30,min
R1834	T2802	T2786	punct	.,added
R1835	T2804	T2805	det	The,reactions
R1836	T2805	T2806	nsubjpass	reactions,terminated
R1837	T2807	T2806	auxpass	were,terminated
R1838	T2808	T2806	agent	by,terminated
R1839	T2809	T2808	pobj	addition,by
R1840	T2810	T2809	prep	of,addition
R1841	T2811	T2810	pobj	EDTA,of
R1842	T2812	T2813	punct	(,mM
R1843	T2813	T2811	parataxis	mM,EDTA
R1844	T2814	T2813	nummod	20,mM
R1845	T2815	T2813	punct	),mM
R1846	T2816	T2811	punct	", ",EDTA
R1847	T2817	T2811	conj	SDS,EDTA
R1848	T2818	T2819	punct	(,%
R1849	T2819	T2817	parataxis	%,SDS
R1850	T2820	T2819	nummod	0.1,%
R1851	T2821	T2819	punct	),%
R1852	T2822	T2817	cc	and,SDS
R1853	T2823	T2824	nummod	2,μg
R1854	T2824	T2825	compound	μg,K
R1855	T2825	T2817	conj	K,SDS
R1856	T2826	T2825	compound	proteinase,K
R1857	T2827	T2806	punct	", ",terminated
R1858	T2828	T2806	cc	and,terminated
R1859	T2829	T2830	auxpass	were,incubated
R1860	T2830	T2806	conj	incubated,terminated
R1861	T2831	T2830	prep	for,incubated
R1862	T2832	T2833	amod	additional,min
R1863	T2833	T2831	pobj	min,for
R1864	T2834	T2833	nummod	15,min
R1865	T2835	T2806	punct	.,terminated
R1866	T2837	T2838	det	The,samples
R1867	T2838	T2839	nsubjpass	samples,separated
R1868	T2840	T2839	auxpass	were,separated
R1869	T2841	T2839	prep	by,separated
R1870	T2842	T2841	pobj	electrophoresis,by
R1871	T2843	T2839	prep	on,separated
R1872	T2844	T2845	amod	nondenaturing,gel
R1873	T2845	T2843	pobj	gel,on
R1874	T2846	T2845	compound	polyacrylamide,gel
R1875	T2847	T2845	prep	in,gel
R1876	T2848	T2849	nummod	1,TBE
R1877	T2849	T2847	pobj	TBE,in
R1878	T2850	T2848	punct	×,1
R1879	T2851	T2839	punct	.,separated
R1880	T2967	T2968	compound	Nuclease,protection
R1881	T2968	T2969	compound	protection,assays
R1882	T2971	T2972	det	The,mixtures
R1883	T2972	T2974	nsubj	mixtures,contained
R1884	T2973	T2972	compound	reaction,mixtures
R1885	T2975	T2976	punct	(,μl
R1886	T2976	T2972	parataxis	μl,mixtures
R1887	T2977	T2976	nummod	25,μl
R1888	T2978	T2976	punct	),μl
R1889	T2979	T2980	nummod	25,mM
R1890	T2980	T2981	compound	mM,NaOH
R1891	T2981	T2974	dobj	NaOH,contained
R1892	T2982	T2981	compound	HEPES,NaOH
R1893	T2983	T2981	punct	–,NaOH
R1894	T2984	T2985	punct	(,pH
R1895	T2985	T2981	parataxis	pH,NaOH
R1896	T2986	T2985	nummod	7.5,pH
R1897	T2987	T2985	punct	),pH
R1898	T2988	T2981	punct	", ",NaOH
R1899	T2989	T2990	nummod	50,mM
R1900	T2990	T2991	compound	mM,acetate
R1901	T2991	T2981	conj	acetate,NaOH
R1902	T2992	T2991	compound	sodium,acetate
R1903	T2993	T2991	punct	", ",acetate
R1904	T2994	T2995	nummod	10,mM
R1905	T2995	T2996	nmod	mM,CH3COO
R1906	T2996	T2991	conj	CH3COO,acetate
R1907	T2997	T2996	nmod	Mg,CH3COO
R1908	T2998	T2996	punct	(,CH3COO
R1909	T2999	T2996	punct	),CH3COO
R1910	T3000	T2996	nummod	2,CH3COO
R1911	T3001	T2996	punct	", ",CH3COO
R1912	T3002	T3003	nummod	1,mM
R1913	T3003	T3004	compound	mM,DTT
R1914	T3004	T2996	conj	DTT,CH3COO
R1915	T3005	T3004	punct	", ",DTT
R1916	T3006	T3007	nummod	0.25,mg
R1917	T3007	T3008	nmod	mg,BSA
R1918	T3008	T3004	conj	BSA,DTT
R1919	T3009	T3010	punct	/,ml
R1920	T3010	T3007	prep	ml,mg
R1921	T3011	T3008	punct	", ",BSA
R1922	T3012	T3013	nummod	0.5,mM
R1923	T3013	T3014	nmod	mM,S
R1924	T3014	T3008	conj	S,BSA
R1925	T3015	T3014	nmod	ATP,S
R1926	T3016	T3014	punct	-,S
R1927	T3017	T3014	punct	γ,S
R1928	T3018	T3014	punct	-,S
R1929	T3019	T3014	punct	", ",S
R1930	T3020	T3021	nummod	0.5,mM
R1931	T3021	T3022	compound	mM,CaCl2
R1932	T3022	T3014	conj	CaCl2,S
R1933	T3023	T3022	punct	", ",CaCl2
R1934	T3024	T3025	nummod	10,fmol
R1935	T3025	T3022	conj	fmol,CaCl2
R1936	T3026	T3025	prep	of,fmol
R1937	T3027	T3028	npadvmod	32P,labeled
R1938	T3028	T3030	amod	labeled,substrate
R1939	T3029	T3028	punct	-,labeled
R1940	T3030	T3026	pobj	substrate,of
R1941	T3031	T3030	compound	bubble,substrate
R1942	T3032	T3025	punct	", ",fmol
R1943	T3033	T3025	cc	and,fmol
R1944	T3034	T3035	det	the,amount
R1945	T3035	T3025	conj	amount,fmol
R1946	T3036	T3035	amod	indicated,amount
R1947	T3037	T3035	prep	of,amount
R1948	T3038	T3039	nmod	Mcm4,proteins
R1949	T3039	T3037	pobj	proteins,of
R1950	T3040	T3038	punct	/,Mcm4
R1951	T3041	T3038	nummod	6,Mcm4
R1952	T3042	T3038	punct	/,Mcm4
R1953	T3043	T3038	nummod	7,Mcm4
R1954	T3044	T2974	punct	.,contained
R1955	T3046	T3047	prep	After,added
R1956	T3048	T3046	pobj	incubation,After
R1957	T3049	T3048	prep	at,incubation
R1958	T3050	T3051	nummod	30,°C
R1959	T3051	T3049	pobj	°C,at
R1960	T3052	T3048	prep	for,incubation
R1961	T3053	T3054	nummod	30,min
R1962	T3054	T3052	pobj	min,for
R1963	T3055	T3047	punct	", ",added
R1964	T3056	T3057	det	the,amount
R1965	T3057	T3047	nsubjpass	amount,added
R1966	T3058	T3057	amod	indicated,amount
R1967	T3059	T3057	prep	of,amount
R1968	T3060	T3059	pobj	DNase,of
R1969	T3061	T3060	nummod	I,DNase
R1970	T3062	T3063	punct	(,Biomedical
R1971	T3063	T3060	parataxis	Biomedical,DNase
R1972	T3064	T3063	compound	TAKARA,Biomedical
R1973	T3065	T3063	punct	", ",Biomedical
R1974	T3066	T3063	npadvmod	Japan,Biomedical
R1975	T3067	T3063	punct	),Biomedical
R1976	T3068	T3047	auxpass	was,added
R1977	T3069	T3047	punct	", ",added
R1978	T3070	T3047	cc	and,added
R1979	T3071	T3072	det	the,mixtures
R1980	T3072	T3073	nsubjpass	mixtures,incubated
R1981	T3073	T3047	conj	incubated,added
R1982	T3074	T3073	auxpass	were,incubated
R1983	T3075	T3073	prep	at,incubated
R1984	T3076	T3077	compound	room,temperature
R1985	T3077	T3075	pobj	temperature,at
R1986	T3078	T3073	prep	for,incubated
R1987	T3079	T3080	nummod	1,min
R1988	T3080	T3078	pobj	min,for
R1989	T3081	T3047	punct	.,added
R1990	T3083	T3084	nsubjpass	DNase,inactivated
R1991	T3085	T3083	nummod	I,DNase
R1992	T3086	T3084	auxpass	was,inactivated
R1993	T3087	T3084	agent	by,inactivated
R1994	T3088	T3089	det	the,addition
R1995	T3089	T3087	pobj	addition,by
R1996	T3090	T3089	prep	of,addition
R1997	T3091	T3092	nummod	250,μl
R1998	T3092	T3090	pobj	μl,of
R1999	T3093	T3092	prep	of,μl
R2000	T3094	T3095	compound	stop,solution
R2001	T3095	T3093	pobj	solution,of
R2002	T3096	T3097	punct	(,EDTA
R2003	T3097	T3084	parataxis	EDTA,inactivated
R2004	T3098	T3099	nummod	20,mM
R2005	T3099	T3097	compound	mM,EDTA
R2006	T3100	T3097	punct	", ",EDTA
R2007	T3101	T3102	compound	0.2M,chloride
R2008	T3102	T3097	conj	chloride,EDTA
R2009	T3103	T3102	compound	sodium,chloride
R2010	T3104	T3102	punct	", ",chloride
R2011	T3105	T3106	nummod	1,%
R2012	T3106	T3107	compound	%,SDS
R2013	T3107	T3102	conj	SDS,chloride
R2014	T3108	T3107	cc	and,SDS
R2015	T3109	T3110	nummod	12.5,μg
R2016	T3110	T3111	nmod	μg,RNA
R2017	T3111	T3107	conj	RNA,SDS
R2018	T3112	T3113	punct	/,ml
R2019	T3113	T3110	prep	ml,μg
R2020	T3114	T3111	compound	yeast,RNA
R2021	T3115	T3111	punct	),RNA
R2022	T3116	T3084	punct	.,inactivated
R2023	T3118	T3119	prep	For,carried
R2024	T3120	T3121	compound	nuclease,footprinting
R2025	T3121	T3123	compound	footprinting,assay
R2026	T3122	T3121	compound	P1,footprinting
R2027	T3123	T3118	pobj	assay,For
R2028	T3124	T3119	punct	", ",carried
R2029	T3125	T3126	compound	binding,reactions
R2030	T3126	T3119	nsubjpass	reactions,carried
R2031	T3127	T3119	auxpass	were,carried
R2032	T3128	T3119	prt	out,carried
R2033	T3129	T3119	prep	under,carried
R2034	T3130	T3131	det	the,conditions
R2035	T3131	T3129	pobj	conditions,under
R2036	T3132	T3131	amod	same,conditions
R2037	T3133	T3131	prep	except,conditions
R2038	T3134	T3135	mark	that,omitted
R2039	T3135	T3133	pcomp	omitted,except
R2040	T3136	T3135	nsubjpass	CaCl2,omitted
R2041	T3137	T3135	auxpass	was,omitted
R2042	T3138	T3119	punct	.,carried
R2043	T3140	T3141	compound	P1,nuclease
R2044	T3141	T3142	nsubjpass	nuclease,added
R2045	T3143	T3144	punct	(,Roche
R2046	T3144	T3141	parataxis	Roche,nuclease
R2047	T3145	T3146	nummod	0.3,U
R2048	T3146	T3144	dep	U,Roche
R2049	T3147	T3144	punct	", ",Roche
R2050	T3148	T3144	punct	),Roche
R2051	T3149	T3142	auxpass	was,added
R2052	T3150	T3142	cc	and,added
R2053	T3151	T3152	nsubjpass	incubation,continued
R2054	T3152	T3142	conj	continued,added
R2055	T3153	T3152	auxpass	was,continued
R2056	T3154	T3152	prep	at,continued
R2057	T3155	T3156	nummod	37,°C
R2058	T3156	T3154	pobj	°C,at
R2059	T3157	T3152	prep	for,continued
R2060	T3158	T3159	nummod	1,min
R2061	T3159	T3157	pobj	min,for
R2062	T3160	T3152	punct	.,continued
R2063	T3162	T3163	nsubjpass	Cleavage,stopped
R2064	T3163	T3165	ccomp	stopped,incubated
R2065	T3164	T3163	auxpass	was,stopped
R2066	T3166	T3163	prep	by,stopped
R2067	T3167	T3166	pobj	addition,by
R2068	T3168	T3167	prep	of,addition
R2069	T3169	T3170	nummod	50,μl
R2070	T3170	T3168	pobj	μl,of
R2071	T3171	T3170	prep	of,μl
R2072	T3172	T3173	nummod	25,mM
R2073	T3173	T3174	compound	mM,EDTA
R2074	T3174	T3171	pobj	EDTA,of
R2075	T3175	T3174	cc	and,EDTA
R2076	T3176	T3177	nummod	0.4,M
R2077	T3177	T3178	compound	M,NaOH
R2078	T3178	T3174	conj	NaOH,EDTA
R2079	T3179	T3165	punct	;,incubated
R2080	T3180	T3181	det	the,mixture
R2081	T3181	T3165	nsubjpass	mixture,incubated
R2082	T3182	T3165	auxpass	was,incubated
R2083	T3183	T3165	prep	at,incubated
R2084	T3184	T3185	compound	room,temperature
R2085	T3185	T3183	pobj	temperature,at
R2086	T3186	T3165	prep	for,incubated
R2087	T3187	T3188	nummod	10,min
R2088	T3188	T3186	pobj	min,for
R2089	T3189	T3188	acl	followed,min
R2090	T3190	T3189	agent	by,followed
R2091	T3191	T3192	det	the,addition
R2092	T3192	T3190	pobj	addition,by
R2093	T3193	T3192	prep	of,addition
R2094	T3194	T3195	nummod	12,μl
R2095	T3195	T3193	pobj	μl,of
R2096	T3196	T3195	prep	of,μl
R2097	T3197	T3198	nummod	3,M
R2098	T3198	T3199	compound	M,acetate
R2099	T3199	T3196	pobj	acetate,of
R2100	T3200	T3199	compound	sodium,acetate
R2101	T3201	T3202	punct	(,pH
R2102	T3202	T3199	parataxis	pH,acetate
R2103	T3203	T3202	nummod	4.8,pH
R2104	T3204	T3202	punct	),pH
R2105	T3205	T3165	punct	.,incubated
R2106	T3207	T3208	nsubjpass	Proteins,removed
R2107	T3209	T3208	auxpass	were,removed
R2108	T3210	T3208	prep	by,removed
R2109	T3211	T3212	compound	phenol,chloroform
R2110	T3212	T3214	compound	chloroform,extraction
R2111	T3213	T3212	punct	–,chloroform
R2112	T3214	T3210	pobj	extraction,by
R2113	T3215	T3208	punct	", ",removed
R2114	T3216	T3208	cc	and,removed
R2115	T3217	T3218	nsubjpass	DNAs,collected
R2116	T3218	T3208	conj	collected,removed
R2117	T3219	T3218	auxpass	were,collected
R2118	T3220	T3218	prep	by,collected
R2119	T3221	T3222	compound	ethanol,precipitation
R2120	T3222	T3220	pobj	precipitation,by
R2121	T3223	T3222	acl	followed,precipitation
R2122	T3224	T3223	agent	by,followed
R2123	T3225	T3224	pobj	wash,by
R2124	T3226	T3225	prep	with,wash
R2125	T3227	T3228	nummod	70,%
R2126	T3228	T3229	compound	%,ethanol
R2127	T3229	T3226	pobj	ethanol,with
R2128	T3230	T3218	punct	.,collected
R2129	T3232	T3233	det	The,DNA
R2130	T3233	T3235	nsubjpass	DNA,subjected
R2131	T3234	T3233	amod	digested,DNA
R2132	T3236	T3235	auxpass	was,subjected
R2133	T3237	T3235	prep	to,subjected
R2134	T3238	T3237	pobj	electrophoresis,to
R2135	T3239	T3238	prep	through,electrophoresis
R2136	T3240	T3241	det	a,gel
R2137	T3241	T3239	pobj	gel,through
R2138	T3242	T3243	nummod	10,%
R2139	T3243	T3241	compound	%,gel
R2140	T3244	T3242	cc	or,10
R2141	T3245	T3242	conj	12,10
R2142	T3246	T3241	compound	polyacrylamide,gel
R2143	T3247	T3241	compound	sequencing,gel
R2144	T3248	T3241	acl	containing,gel
R2145	T3249	T3250	nummod	7,M
R2146	T3250	T3251	compound	M,urea
R2147	T3251	T3248	dobj	urea,containing
R2148	T3252	T3238	punct	", ",electrophoresis
R2149	T3253	T3238	acl	followed,electrophoresis
R2150	T3254	T3253	agent	by,followed
R2151	T3255	T3254	pobj	autoradiography,by
R2152	T3256	T3235	punct	.,subjected
R2153	T3428	T3429	nmod	Mcm4,complex
R2154	T3429	T3434	nsubj	complex,interacts
R2155	T3430	T3428	punct	/,Mcm4
R2156	T3431	T3428	nummod	6,Mcm4
R2157	T3432	T3428	punct	/,Mcm4
R2158	T3433	T3428	nummod	7,Mcm4
R2159	T3435	T3434	advmod	primarily,interacts
R2160	T3436	T3434	prep	with,interacts
R2161	T3437	T3438	det	the,segment
R2162	T3438	T3436	pobj	segment,with
R2163	T3439	T3440	amod	single,stranded
R2164	T3440	T3438	amod	stranded,segment
R2165	T3441	T3440	punct	-,stranded
R2166	T3442	T3438	compound	DNA,segment
R2167	T3444	T3445	nsubj	Mcm4,binds
R2168	T3446	T3444	punct	/,Mcm4
R2169	T3447	T3444	nummod	6,Mcm4
R2170	T3448	T3444	punct	/,Mcm4
R2171	T3449	T3444	nummod	7,Mcm4
R2172	T3450	T3445	prep	to,binds
R2173	T3451	T3452	punct	‘,substrates
R2174	T3452	T3450	pobj	substrates,to
R2175	T3453	T3452	nmod	bubble,substrates
R2176	T3454	T3452	punct	’,substrates
R2177	T3455	T3452	compound	DNA,substrates
R2178	T3456	T3445	cc	and,binds
R2179	T3457	T3445	conj	unwinds,binds
R2180	T3458	T3459	det	the,segments
R2181	T3459	T3457	dobj	segments,unwinds
R2182	T3460	T3459	compound	duplex,segments
R2183	T3461	T3462	advmod	when,contains
R2184	T3462	T3457	advcl	contains,unwinds
R2185	T3463	T3464	det	the,segment
R2186	T3464	T3462	nsubj	segment,contains
R2187	T3465	T3464	amod	central,segment
R2188	T3466	T3467	amod	single,stranded
R2189	T3467	T3464	amod	stranded,segment
R2190	T3468	T3467	punct	-,stranded
R2191	T3469	T3470	compound	thymine,stretches
R2192	T3470	T3462	dobj	stretches,contains
R2193	T3471	T3472	punct	(,13
R2194	T3472	T3462	parataxis	13,contains
R2195	T3473	T3472	punct	),13
R2196	T3474	T3445	punct	.,binds
R2197	T3476	T3477	prep	In,conducted
R2198	T3478	T3476	pobj	order,In
R2199	T3479	T3480	aux	to,examine
R2200	T3480	T3478	acl	examine,order
R2201	T3481	T3482	det	the,mode
R2202	T3482	T3480	dobj	mode,examine
R2203	T3483	T3482	prep	of,mode
R2204	T3484	T3483	pobj	binding,of
R2205	T3485	T3484	prep	of,binding
R2206	T3486	T3485	pobj	Mcm4,of
R2207	T3487	T3486	punct	/,Mcm4
R2208	T3488	T3486	nummod	6,Mcm4
R2209	T3489	T3486	punct	/,Mcm4
R2210	T3490	T3486	nummod	7,Mcm4
R2211	T3491	T3484	prep	on,binding
R2212	T3492	T3493	compound	bubble,DNAs
R2213	T3493	T3491	pobj	DNAs,on
R2214	T3494	T3477	punct	", ",conducted
R2215	T3495	T3477	nsubj	we,conducted
R2216	T3496	T3497	compound	nuclease,assays
R2217	T3497	T3477	dobj	assays,conducted
R2218	T3498	T3497	compound	protection,assays
R2219	T3499	T3477	advcl	using,conducted
R2220	T3500	T3501	amod	labeled,DNAs
R2221	T3501	T3499	dobj	DNAs,using
R2222	T3502	T3503	compound	bubble,substrate
R2223	T3503	T3501	compound	substrate,DNAs
R2224	T3504	T3501	cc	and,DNAs
R2225	T3505	T3501	conj	Mcm4,DNAs
R2226	T3506	T3505	punct	/,Mcm4
R2227	T3507	T3505	nummod	6,Mcm4
R2228	T3508	T3505	punct	/,Mcm4
R2229	T3509	T3505	nummod	7,Mcm4
R2230	T3510	T3477	prep	in,conducted
R2231	T3511	T3512	det	the,presence
R2232	T3512	T3510	pobj	presence,in
R2233	T3513	T3512	prep	of,presence
R2234	T3514	T3515	nmod	ATP,S
R2235	T3515	T3513	pobj	S,of
R2236	T3516	T3515	punct	-,S
R2237	T3517	T3515	punct	γ,S
R2238	T3518	T3515	punct	-,S
R2239	T3519	T3520	dep	which,permits
R2240	T3520	T3515	relcl	permits,S
R2241	T3521	T3520	dobj	binding,permits
R2242	T3522	T3521	prep	to,binding
R2243	T3523	T3524	compound	substrate,DNA
R2244	T3524	T3522	pobj	DNA,to
R2245	T3525	T3520	cc	but,permits
R2246	T3526	T3527	aux	does,mobilize
R2247	T3527	T3520	conj	mobilize,permits
R2248	T3528	T3527	neg	not,mobilize
R2249	T3529	T3530	det	the,helicase
R2250	T3530	T3527	dobj	helicase,mobilize
R2251	T3531	T3477	punct	.,conducted
R2252	T3533	T3534	prep	At,detected
R2253	T3535	T3536	det	an,concentration
R2254	T3536	T3533	pobj	concentration,At
R2255	T3537	T3536	amod	optimum,concentration
R2256	T3538	T3536	prep	of,concentration
R2257	T3539	T3538	pobj	DNase,of
R2258	T3540	T3539	nummod	I,DNase
R2259	T3541	T3534	punct	", ",detected
R2260	T3542	T3543	amod	strong,cleavages
R2261	T3543	T3534	nsubjpass	cleavages,detected
R2262	T3544	T3534	auxpass	were,detected
R2263	T3545	T3534	prep	on,detected
R2264	T3546	T3547	det	the,regions
R2265	T3547	T3545	pobj	regions,on
R2266	T3548	T3549	nummod	21,bp
R2267	T3549	T3547	compound	bp,regions
R2268	T3550	T3547	compound	duplex,regions
R2269	T3551	T3534	prep	at,detected
R2270	T3552	T3553	det	both,ends
R2271	T3553	T3551	pobj	ends,at
R2272	T3554	T3553	prep	of,ends
R2273	T3555	T3556	det	the,substrates
R2274	T3556	T3554	pobj	substrates,of
R2275	T3557	T3534	punct	", ",detected
R2276	T3558	T3559	mark	whereas,detected
R2277	T3559	T3534	advcl	detected,detected
R2278	T3560	T3561	amod	weaker,cleavages
R2279	T3561	T3559	nsubjpass	cleavages,detected
R2280	T3562	T3559	auxpass	were,detected
R2281	T3563	T3559	prep	on,detected
R2282	T3564	T3565	det	the,region
R2283	T3565	T3563	pobj	region,on
R2284	T3566	T3565	amod	central,region
R2285	T3567	T3568	amod	single,stranded
R2286	T3568	T3565	amod	stranded,region
R2287	T3569	T3568	punct	-,stranded
R2288	T3570	T3559	punct	", ",detected
R2289	T3571	T3559	advcl	consistent,detected
R2290	T3572	T3571	prep	with,consistent
R2291	T3573	T3572	pobj	preference,with
R2292	T3574	T3573	prep	of,preference
R2293	T3575	T3574	pobj	DNase,of
R2294	T3576	T3575	nummod	I,DNase
R2295	T3577	T3573	prep	to,preference
R2296	T3578	T3579	amod	double,stranded
R2297	T3579	T3581	amod	stranded,DNA
R2298	T3580	T3579	punct	-,stranded
R2299	T3581	T3577	pobj	DNA,to
R2300	T3582	T3534	punct	.,detected
R2301	T3584	T3585	prep	With,detected
R2302	T3586	T3587	det	the,substrate
R2303	T3587	T3584	pobj	substrate,With
R2304	T3588	T3587	nmod	Bub66,substrate
R2305	T3589	T3588	punct	/,Bub66
R2306	T3590	T3588	appos	T,Bub66
R2307	T3591	T3590	punct	-,T
R2308	T3592	T3590	amod	rich,T
R2309	T3593	T3585	punct	", ",detected
R2310	T3594	T3595	amod	strong,protection
R2311	T3595	T3585	nsubjpass	protection,detected
R2312	T3596	T3585	auxpass	was,detected
R2313	T3597	T3585	prep	on,detected
R2314	T3598	T3599	det	the,regions
R2315	T3599	T3597	pobj	regions,on
R2316	T3600	T3599	amod	entire,regions
R2317	T3601	T3602	amod	single,stranded
R2318	T3602	T3599	amod	stranded,regions
R2319	T3603	T3602	punct	-,stranded
R2320	T3604	T3599	prep	of,regions
R2321	T3605	T3606	preconj	both,top
R2322	T3606	T3607	amod	top,strands
R2323	T3607	T3604	pobj	strands,of
R2324	T3608	T3606	cc	and,top
R2325	T3609	T3606	conj	bottom,top
R2326	T3610	T3585	prep	with,detected
R2327	T3611	T3612	amod	increasing,concentration
R2328	T3612	T3610	pobj	concentration,with
R2329	T3613	T3612	prep	of,concentration
R2330	T3614	T3613	pobj	Mcm4,of
R2331	T3615	T3614	punct	/,Mcm4
R2332	T3616	T3614	nummod	6,Mcm4
R2333	T3617	T3614	punct	/,Mcm4
R2334	T3618	T3614	nummod	7,Mcm4
R2335	T3619	T3585	punct	", ",detected
R2336	T3620	T3585	advcl	consistent,detected
R2337	T3621	T3620	prep	with,consistent
R2338	T3622	T3623	amod	high,affinity
R2339	T3623	T3621	pobj	affinity,with
R2340	T3624	T3623	prep	of,affinity
R2341	T3625	T3624	pobj	Mcm4,of
R2342	T3626	T3625	punct	/,Mcm4
R2343	T3627	T3625	nummod	6,Mcm4
R2344	T3628	T3625	punct	/,Mcm4
R2345	T3629	T3625	nummod	7,Mcm4
R2346	T3630	T3623	prep	to,affinity
R2347	T3631	T3632	nmod	T,sequences
R2348	T3632	T3630	pobj	sequences,to
R2349	T3633	T3631	punct	-,T
R2350	T3634	T3631	amod	rich,T
R2351	T3635	T3632	compound	bubble,sequences
R2352	T3636	T3637	punct	(,1A
R2353	T3637	T3585	parataxis	1A,detected
R2354	T3638	T3637	compound	Figure,1A
R2355	T3639	T3637	punct	),1A
R2356	T3640	T3585	punct	.,detected
R2357	T3642	T3643	amod	Similar,protection
R2358	T3643	T3648	nsubjpass	protection,detected
R2359	T3644	T3642	punct	", ",Similar
R2360	T3645	T3642	cc	but,Similar
R2361	T3646	T3647	advmod	less,significant
R2362	T3647	T3642	conj	significant,Similar
R2363	T3649	T3648	auxpass	was,detected
R2364	T3650	T3648	prep	on,detected
R2365	T3651	T3652	det	the,segment
R2366	T3652	T3650	pobj	segment,on
R2367	T3653	T3654	amod	single,stranded
R2368	T3654	T3652	amod	stranded,segment
R2369	T3655	T3654	punct	-,stranded
R2370	T3656	T3652	compound	DNA,segment
R2371	T3657	T3652	prep	with,segment
R2372	T3658	T3659	compound	P1,nucleases
R2373	T3659	T3657	pobj	nucleases,with
R2374	T3660	T3661	punct	(,1A
R2375	T3661	T3652	parataxis	1A,segment
R2376	T3662	T3661	compound	Figure,1A
R2377	T3663	T3661	punct	),1A
R2378	T3664	T3652	punct	", ",segment
R2379	T3665	T3666	dep	which,is
R2380	T3666	T3652	relcl	is,segment
R2381	T3667	T3666	acomp	specific,is
R2382	T3668	T3667	prep	to,specific
R2383	T3669	T3670	amod	single,stranded
R2384	T3670	T3672	amod	stranded,DNA
R2385	T3671	T3670	punct	-,stranded
R2386	T3672	T3668	pobj	DNA,to
R2387	T3673	T3648	punct	.,detected
R2388	T3675	T3676	det	The,results
R2389	T3676	T3677	nsubj	results,indicate
R2390	T3678	T3679	mark	that,loaded
R2391	T3679	T3677	ccomp	loaded,indicate
R2392	T3680	T3681	det	the,complex
R2393	T3681	T3679	nsubjpass	complex,loaded
R2394	T3682	T3681	compound	Mcm,complex
R2395	T3683	T3679	auxpass	is,loaded
R2396	T3684	T3679	prep	onto,loaded
R2397	T3685	T3686	compound	bubble,DNA
R2398	T3686	T3684	pobj	DNA,onto
R2399	T3687	T3679	prep	through,loaded
R2400	T3688	T3687	pobj	binding,through
R2401	T3689	T3688	prep	to,binding
R2402	T3690	T3691	det	both,strands
R2403	T3691	T3689	pobj	strands,to
R2404	T3692	T3691	prep	of,strands
R2405	T3693	T3694	det	the,region
R2406	T3694	T3692	pobj	region,of
R2407	T3695	T3696	amod	single,stranded
R2408	T3696	T3694	amod	stranded,region
R2409	T3697	T3696	punct	-,stranded
R2410	T3698	T3677	punct	.,indicate
R2411	T3700	T3701	amod	Careful,examination
R2412	T3701	T3702	nsubj	examination,indicated
R2413	T3703	T3701	prep	of,examination
R2414	T3704	T3705	nmod	Mcm4,footprints
R2415	T3705	T3703	pobj	footprints,of
R2416	T3706	T3704	punct	/,Mcm4
R2417	T3707	T3704	nummod	6,Mcm4
R2418	T3708	T3704	punct	/,Mcm4
R2419	T3709	T3704	nummod	7,Mcm4
R2420	T3710	T3705	prep	on,footprints
R2421	T3711	T3712	det	a,bubble
R2422	T3712	T3710	pobj	bubble,on
R2423	T3713	T3714	amod	strong,protection
R2424	T3714	T3702	dobj	protection,indicated
R2425	T3715	T3714	prep	on,protection
R2426	T3716	T3717	det	the,half
R2427	T3717	T3715	pobj	half,on
R2428	T3718	T3717	nummod	5,half
R2429	T3719	T3718	punct	′,5
R2430	T3720	T3717	punct	-,half
R2431	T3721	T3717	prep	of,half
R2432	T3722	T3723	det	the,strand
R2433	T3723	T3721	pobj	strand,of
R2434	T3724	T3723	amod	top,strand
R2435	T3725	T3714	cc	and,protection
R2436	T3726	T3727	amod	moderate,protection
R2437	T3727	T3714	conj	protection,protection
R2438	T3728	T3727	prep	on,protection
R2439	T3729	T3730	det	the,half
R2440	T3730	T3728	pobj	half,on
R2441	T3731	T3730	amod	remaining,half
R2442	T3732	T3730	nummod	3,half
R2443	T3733	T3732	punct	′,3
R2444	T3734	T3730	punct	-,half
R2445	T3735	T3728	punct	", ",on
R2446	T3736	T3737	advmod	as,as
R2447	T3737	T3728	cc	as,on
R2448	T3738	T3737	advmod	well,as
R2449	T3739	T3728	conj	on,on
R2450	T3740	T3741	amod	several,segment
R2451	T3741	T3739	pobj	segment,on
R2452	T3742	T3743	compound	base,pair
R2453	T3743	T3741	compound	pair,segment
R2454	T3744	T3743	punct	-,pair
R2455	T3745	T3741	compound	duplex,segment
R2456	T3746	T3741	amod	adjacent,segment
R2457	T3747	T3746	prep	to,adjacent
R2458	T3748	T3749	det	the,point
R2459	T3749	T3747	pobj	point,to
R2460	T3750	T3749	compound	branch,point
R2461	T3751	T3752	punct	(,1A
R2462	T3752	T3739	parataxis	1A,on
R2463	T3753	T3752	compound	Figure,1A
R2464	T3754	T3752	cc	and,1A
R2465	T3755	T3752	conj	data,1A
R2466	T3756	T3757	neg	not,shown
R2467	T3757	T3755	acl	shown,data
R2468	T3758	T3752	punct	),1A
R2469	T3759	T3702	punct	.,indicated
R2470	T3761	T3762	det	A,pattern
R2471	T3762	T3764	nsubjpass	pattern,observed
R2472	T3763	T3762	amod	similar,pattern
R2473	T3765	T3762	prep	of,pattern
R2474	T3766	T3765	pobj	protection,of
R2475	T3767	T3764	auxpass	was,observed
R2476	T3768	T3764	advmod	also,observed
R2477	T3769	T3764	prep	on,observed
R2478	T3770	T3771	det	the,strand
R2479	T3771	T3769	pobj	strand,on
R2480	T3772	T3771	amod	bottom,strand
R2481	T3773	T3764	punct	.,observed
R2482	T3775	T3776	nsubj	This,suggests
R2483	T3777	T3778	mark	that,bind
R2484	T3778	T3776	ccomp	bind,suggests
R2485	T3779	T3778	nsubj	Mcm4,bind
R2486	T3780	T3779	punct	/,Mcm4
R2487	T3781	T3779	nummod	6,Mcm4
R2488	T3782	T3779	punct	/,Mcm4
R2489	T3783	T3779	nummod	7,Mcm4
R2490	T3784	T3778	aux	may,bind
R2491	T3785	T3778	prep	to,bind
R2492	T3786	T3787	det	a,substrate
R2493	T3787	T3785	pobj	substrate,to
R2494	T3788	T3787	compound	replication,substrate
R2495	T3789	T3787	compound	bubble,substrate
R2496	T3790	T3787	prep	with,substrate
R2497	T3791	T3792	det	a,symmetry
R2498	T3792	T3790	pobj	symmetry,with
R2499	T3793	T3792	nummod	2-fold,symmetry
R2500	T3794	T3778	prep	as,bind
R2501	T3795	T3796	det	a,hexamer
R2502	T3796	T3794	pobj	hexamer,as
R2503	T3797	T3796	amod	double,hexamer
R2504	T3798	T3799	punct	(,1A
R2505	T3799	T3796	parataxis	1A,hexamer
R2506	T3800	T3799	compound	Figure,1A
R2507	T3801	T3799	punct	", ",1A
R2508	T3802	T3799	advcl	see,1A
R2509	T3803	T3804	det	the,scheme
R2510	T3804	T3802	dobj	scheme,see
R2511	T3805	T3804	amod	lower,scheme
R2512	T3806	T3799	punct	),1A
R2513	T3807	T3778	punct	", ",bind
R2514	T3808	T3778	prep	in,bind
R2515	T3809	T3810	det	a,manner
R2516	T3810	T3808	pobj	manner,in
R2517	T3811	T3810	amod	similar,manner
R2518	T3812	T3811	prep	to,similar
R2519	T3813	T3814	compound	SV40,antigen
R2520	T3814	T3812	pobj	antigen,to
R2521	T3815	T3814	compound	T,antigen
R2522	T3816	T3814	punct	-,antigen
R2523	T3817	T3818	punct	(,21
R2524	T3818	T3778	parataxis	21,bind
R2525	T3819	T3818	punct	),21
R2526	T3820	T3776	punct	.,suggests
R2527	T3822	T3823	compound	Nuclease,protection
R2528	T3823	T3824	compound	protection,assays
R2529	T3824	T3825	nsubjpass	assays,conducted
R2530	T3826	T3825	auxpass	were,conducted
R2531	T3827	T3825	advmod	also,conducted
R2532	T3828	T3825	prep	on,conducted
R2533	T3829	T3830	det	a,structure
R2534	T3830	T3828	pobj	structure,on
R2535	T3831	T3832	npadvmod	T,tailed
R2536	T3832	T3830	amod	tailed,structure
R2537	T3833	T3832	punct	-,tailed
R2538	T3834	T3835	compound	Y,fork
R2539	T3835	T3830	compound	fork,structure
R2540	T3836	T3835	punct	-,fork
R2541	T3837	T3838	punct	(,1B
R2542	T3838	T3825	parataxis	1B,conducted
R2543	T3839	T3838	compound	Figure,1B
R2544	T3840	T3838	punct	),1B
R2545	T3841	T3825	punct	.,conducted
R2546	T3843	T3844	preconj	Not,region
R2547	T3844	T3851	nsubjpass	region,protected
R2548	T3845	T3843	advmod	only,Not
R2549	T3846	T3844	det	the,region
R2550	T3847	T3848	amod	single,stranded
R2551	T3848	T3844	amod	stranded,region
R2552	T3849	T3848	punct	-,stranded
R2553	T3850	T3844	compound	tail,region
R2554	T3852	T3844	cc	but,region
R2555	T3853	T3852	advmod	also,but
R2556	T3854	T3855	det	the,DNA
R2557	T3855	T3844	conj	DNA,region
R2558	T3856	T3857	nummod	7,bp
R2559	T3857	T3855	compound	bp,DNA
R2560	T3858	T3855	compound	duplex,DNA
R2561	T3859	T3855	amod	adjacent,DNA
R2562	T3860	T3859	prep	to,adjacent
R2563	T3861	T3862	det	the,junction
R2564	T3862	T3860	pobj	junction,to
R2565	T3863	T3862	nmod	duplex,junction
R2566	T3864	T3863	punct	-,duplex
R2567	T3865	T3863	prep	to,duplex
R2568	T3866	T3865	punct	-,to
R2569	T3867	T3868	amod	single,strand
R2570	T3868	T3865	pobj	strand,to
R2571	T3869	T3868	punct	-,strand
R2572	T3870	T3851	auxpass	were,protected
R2573	T3871	T3851	agent	by,protected
R2574	T3872	T3873	compound	Mcm,binding
R2575	T3873	T3871	pobj	binding,by
R2576	T3874	T3873	prep	from,binding
R2577	T3875	T3876	compound	DNaseI,digestion
R2578	T3876	T3874	pobj	digestion,from
R2579	T3877	T3851	punct	.,protected
R2580	T3879	T3880	det	These,results
R2581	T3880	T3881	nsubj	results,indicate
R2582	T3882	T3883	mark	that,interacts
R2583	T3883	T3881	advcl	interacts,indicate
R2584	T3884	T3885	nmod	Mcm4,protein
R2585	T3885	T3883	nsubj	protein,interacts
R2586	T3886	T3884	punct	/,Mcm4
R2587	T3887	T3884	nummod	6,Mcm4
R2588	T3888	T3884	punct	/,Mcm4
R2589	T3889	T3884	nummod	7,Mcm4
R2590	T3890	T3883	advmod	primarily,interacts
R2591	T3891	T3883	prep	with,interacts
R2592	T3892	T3893	amod	single,stranded
R2593	T3893	T3895	amod	stranded,region
R2594	T3894	T3893	punct	-,stranded
R2595	T3895	T3891	pobj	region,with
R2596	T3896	T3895	compound	DNA,region
R2597	T3897	T3883	cc	and,interacts
R2598	T3898	T3899	mark	that,spans
R2599	T3899	T3883	conj	spans,interacts
R2600	T3900	T3901	det	the,interaction
R2601	T3901	T3899	nsubj	interaction,spans
R2602	T3902	T3899	advmod	also,spans
R2603	T3903	T3904	det	the,segments
R2604	T3904	T3899	dobj	segments,spans
R2605	T3905	T3904	compound	duplex,segments
R2606	T3906	T3904	prep	near,segments
R2607	T3907	T3908	det	the,branchpoint
R2608	T3908	T3906	pobj	branchpoint,near
R2609	T3909	T3908	prep	of,branchpoint
R2610	T3910	T3911	det	the,fork
R2611	T3911	T3909	pobj	fork,of
R2612	T3912	T3911	compound	replication,fork
R2613	T3913	T3881	punct	.,indicate
R2617	T4077	T4078	nsubj	Formation,facilitates
R2618	T4079	T4077	prep	of,Formation
R2619	T4080	T4081	amod	double,hexameric
R2620	T4081	T4082	amod	hexameric,complexes
R2621	T4082	T4079	pobj	complexes,of
R2622	T4083	T4082	nmod	Mcm4,complexes
R2623	T4084	T4083	punct	/,Mcm4
R2624	T4085	T4083	nummod	6,Mcm4
R2625	T4086	T4083	punct	/,Mcm4
R2626	T4087	T4083	nummod	7,Mcm4
R2627	T4088	T4089	compound	helicase,actions
R2628	T4089	T4078	dobj	actions,facilitates
R2629	T4090	T4078	prep	on,facilitates
R2630	T4091	T4092	compound	bubble,substrates
R2631	T4092	T4090	pobj	substrates,on
R2632	T4094	T4095	det	The,results
R2633	T4095	T4097	nsubj	results,suggest
R2634	T4096	T4095	amod	above,results
R2635	T4098	T4099	mark	that,generates
R2636	T4099	T4097	ccomp	generates,suggest
R2637	T4100	T4099	nsubj	Mcm4,generates
R2638	T4101	T4100	punct	/,Mcm4
R2639	T4102	T4100	nummod	6,Mcm4
R2640	T4103	T4100	punct	/,Mcm4
R2641	T4104	T4100	nummod	7,Mcm4
R2642	T4105	T4106	det	a,complex
R2643	T4106	T4099	dobj	complex,generates
R2644	T4107	T4108	amod	double,hexameric
R2645	T4108	T4106	amod	hexameric,complex
R2646	T4109	T4099	prep	on,generates
R2647	T4110	T4111	det	a,bubble
R2648	T4111	T4109	pobj	bubble,on
R2649	T4112	T4099	punct	", ",generates
R2650	T4113	T4114	det	each,hexamer
R2651	T4114	T4115	dep	hexamer,makes
R2652	T4115	T4099	ccomp	makes,generates
R2653	T4116	T4114	prep	of,hexamer
R2654	T4117	T4116	pobj	which,of
R2655	T4118	T4119	amod	symmetric,contact
R2656	T4119	T4115	dobj	contact,makes
R2657	T4120	T4119	prep	with,contact
R2658	T4121	T4122	det	the,segment
R2659	T4122	T4120	pobj	segment,with
R2660	T4123	T4124	npadvmod	5,proximal
R2661	T4124	T4122	amod	proximal,segment
R2662	T4125	T4123	punct	′,5
R2663	T4126	T4124	punct	-,proximal
R2664	T4127	T4122	amod	central,segment
R2665	T4128	T4129	amod	single,stranded
R2666	T4129	T4122	amod	stranded,segment
R2667	T4130	T4129	punct	-,stranded
R2668	T4131	T4097	punct	.,suggest
R2669	T4133	T4134	nsubj	This,predicts
R2670	T4135	T4136	mark	that,needs
R2671	T4136	T4134	ccomp	needs,predicts
R2672	T4137	T4138	det	the,segment
R2673	T4138	T4136	nsubj	segment,needs
R2674	T4139	T4140	amod	single,stranded
R2675	T4140	T4138	amod	stranded,segment
R2676	T4141	T4140	punct	-,stranded
R2677	T4142	T4143	aux	to,be
R2678	T4143	T4136	xcomp	be,needs
R2679	T4144	T4143	prep	of,be
R2680	T4145	T4146	det	a,length
R2681	T4146	T4144	pobj	length,of
R2682	T4147	T4146	amod	sufficient,length
R2683	T4148	T4143	prep	in,be
R2684	T4149	T4148	pobj	order,in
R2685	T4150	T4151	aux	to,accommodate
R2686	T4151	T4149	acl	accommodate,order
R2687	T4152	T4153	det	the,complex
R2688	T4153	T4151	dobj	complex,accommodate
R2689	T4154	T4153	compound	Mcm,complex
R2690	T4155	T4134	punct	.,predicts
R2691	T4157	T4158	prep	In,showed
R2692	T4159	T4157	pobj	fact,In
R2693	T4160	T4158	punct	", ",showed
R2694	T4161	T4158	nsubj	we,showed
R2695	T4162	T4158	advmod	previously,showed
R2696	T4163	T4164	mark	that,forms
R2697	T4164	T4158	ccomp	forms,showed
R2698	T4165	T4166	det	the,Mcm4
R2699	T4166	T4164	nsubj	Mcm4,forms
R2700	T4167	T4166	punct	/,Mcm4
R2701	T4168	T4166	nummod	6,Mcm4
R2702	T4169	T4166	punct	/,Mcm4
R2703	T4170	T4166	nummod	7,Mcm4
R2704	T4171	T4164	advmod	mainly,forms
R2705	T4172	T4173	det	a,complex
R2706	T4173	T4164	dobj	complex,forms
R2707	T4174	T4175	amod	single,hexameric
R2708	T4175	T4173	amod	hexameric,complex
R2709	T4176	T4173	prep	on,complex
R2710	T4177	T4176	pobj	bubble,on
R2711	T4178	T4177	punct	-,bubble
R2712	T4179	T4177	nummod	20,bubble
R2713	T4180	T4177	punct	(,bubble
R2714	T4181	T4182	det	a,substrate
R2715	T4182	T4177	appos	substrate,bubble
R2716	T4183	T4182	compound	bubble,substrate
R2717	T4184	T4182	prep	with,substrate
R2718	T4185	T4186	nummod	20,nt
R2719	T4186	T4187	npadvmod	nt,long
R2720	T4187	T4188	amod	long,segment
R2721	T4188	T4184	pobj	segment,with
R2722	T4189	T4188	amod	central,segment
R2723	T4190	T4191	amod	single,stranded
R2724	T4191	T4188	amod	stranded,segment
R2725	T4192	T4191	punct	-,stranded
R2726	T4193	T4173	punct	),complex
R2727	T4194	T4173	cc	and,complex
R2728	T4195	T4196	det	a,complex
R2729	T4196	T4173	conj	complex,complex
R2730	T4197	T4198	amod	double,hexameric
R2731	T4198	T4196	amod	hexameric,complex
R2732	T4199	T4196	prep	on,complex
R2733	T4200	T4201	nmod	bubble,DNA
R2734	T4201	T4199	pobj	DNA,on
R2735	T4202	T4200	punct	-,bubble
R2736	T4203	T4200	nummod	60,bubble
R2737	T4204	T4205	punct	(,13
R2738	T4205	T4196	parataxis	13,complex
R2739	T4206	T4205	punct	),13
R2740	T4207	T4158	punct	.,showed
R2741	T4209	T4210	prep	In,constructed
R2742	T4211	T4209	pobj	order,In
R2743	T4212	T4213	aux	to,determine
R2744	T4213	T4211	acl	determine,order
R2745	T4214	T4215	det	the,requirement
R2746	T4215	T4213	dobj	requirement,determine
R2747	T4216	T4215	amod	minimum,requirement
R2748	T4217	T4215	prep	for,requirement
R2749	T4218	T4219	amod	double,hexamer
R2750	T4219	T4221	compound	hexamer,formation
R2751	T4220	T4219	punct	-,hexamer
R2752	T4221	T4217	pobj	formation,for
R2753	T4222	T4210	punct	", ",constructed
R2754	T4223	T4224	det	a,set
R2755	T4224	T4210	nsubjpass	set,constructed
R2756	T4225	T4224	prep	of,set
R2757	T4226	T4227	amod	synthetic,substrates
R2758	T4227	T4225	pobj	substrates,of
R2759	T4228	T4229	npadvmod	bubble,like
R2760	T4229	T4227	amod	like,substrates
R2761	T4230	T4229	punct	-,like
R2762	T4231	T4227	punct	(,substrates
R2763	T4232	T4233	compound	Bub,T10
R2764	T4233	T4227	appos	T10,substrates
R2765	T4234	T4233	punct	-,T10
R2766	T4235	T4233	punct	", ",T10
R2767	T4236	T4237	compound	Bub,T20
R2768	T4237	T4233	conj	T20,T10
R2769	T4238	T4237	punct	-,T20
R2770	T4239	T4237	punct	", ",T20
R2771	T4240	T4241	compound	Bub,T30
R2772	T4241	T4237	conj	T30,T20
R2773	T4242	T4241	punct	-,T30
R2774	T4243	T4241	punct	", ",T30
R2775	T4244	T4245	compound	Bub,T40
R2776	T4245	T4241	conj	T40,T30
R2777	T4246	T4245	punct	-,T40
R2778	T4247	T4245	cc	and,T40
R2779	T4248	T4249	compound	Bub,T50
R2780	T4249	T4245	conj	T50,T40
R2781	T4250	T4249	punct	-,T50
R2782	T4251	T4227	punct	),substrates
R2783	T4252	T4253	dep	that,differed
R2784	T4253	T4227	relcl	differed,substrates
R2785	T4254	T4253	prep	in,differed
R2786	T4255	T4256	det	the,length
R2787	T4256	T4254	pobj	length,in
R2788	T4257	T4256	prep	of,length
R2789	T4258	T4259	det	the,segment
R2790	T4259	T4257	pobj	segment,of
R2791	T4260	T4259	amod	central,segment
R2792	T4261	T4259	amod	unpaired,segment
R2793	T4262	T4210	auxpass	were,constructed
R2794	T4263	T4210	punct	", ",constructed
R2795	T4264	T4210	cc	and,constructed
R2796	T4265	T4266	det	the,activities
R2797	T4266	T4270	nsubjpass	activities,examined
R2798	T4267	T4266	nmod	binding,activities
R2799	T4268	T4267	cc	and,binding
R2800	T4269	T4267	conj	helicase,binding
R2801	T4270	T4210	conj	examined,constructed
R2802	T4271	T4266	prep	of,activities
R2803	T4272	T4271	pobj	Mcm4,of
R2804	T4273	T4272	punct	/,Mcm4
R2805	T4274	T4272	nummod	6,Mcm4
R2806	T4275	T4272	punct	/,Mcm4
R2807	T4276	T4272	nummod	7,Mcm4
R2808	T4277	T4266	prep	on,activities
R2809	T4278	T4279	det	these,substrates
R2810	T4279	T4277	pobj	substrates,on
R2811	T4280	T4270	auxpass	were,examined
R2812	T4281	T4210	punct	.,constructed
R2813	T4283	T4284	nsubjpass	Mcm4,incubated
R2814	T4285	T4283	punct	/,Mcm4
R2815	T4286	T4283	nummod	6,Mcm4
R2816	T4287	T4283	punct	/,Mcm4
R2817	T4288	T4283	nummod	7,Mcm4
R2818	T4289	T4284	auxpass	was,incubated
R2819	T4290	T4284	prep	with,incubated
R2820	T4291	T4292	det	a,substrate
R2821	T4292	T4290	pobj	substrate,with
R2822	T4293	T4292	amod	radiolabeled,substrate
R2823	T4294	T4292	amod	synthetic,substrate
R2824	T4295	T4292	compound	bubble,substrate
R2825	T4296	T4284	prep	in,incubated
R2826	T4297	T4298	det	the,presence
R2827	T4298	T4296	pobj	presence,in
R2828	T4299	T4298	prep	of,presence
R2829	T4300	T4301	nummod	0.5,mM
R2830	T4301	T4302	nmod	mM,S
R2831	T4302	T4299	pobj	S,of
R2832	T4303	T4302	nmod	ATP,S
R2833	T4304	T4302	punct	-,S
R2834	T4305	T4302	punct	γ,S
R2835	T4306	T4302	punct	-,S
R2836	T4307	T4308	aux	to,allow
R2837	T4308	T4284	advcl	allow,incubated
R2838	T4309	T4310	compound	complex,formation
R2839	T4310	T4308	dobj	formation,allow
R2840	T4311	T4284	punct	.,incubated
R2841	T4313	T4314	det	A,half
R2842	T4314	T4315	nsubjpass	half,analyzed
R2843	T4316	T4314	prep	of,half
R2844	T4317	T4318	det	these,mixtures
R2845	T4318	T4316	pobj	mixtures,of
R2846	T4319	T4318	compound	reaction,mixtures
R2847	T4320	T4315	auxpass	were,analyzed
R2848	T4321	T4315	prep	for,analyzed
R2849	T4322	T4323	compound	DNA,binding
R2850	T4323	T4321	pobj	binding,for
R2851	T4324	T4315	prep	in,analyzed
R2852	T4325	T4326	compound	gel,shift
R2853	T4326	T4327	compound	shift,assays
R2854	T4327	T4324	pobj	assays,in
R2855	T4328	T4329	punct	(,2A
R2856	T4329	T4315	parataxis	2A,analyzed
R2857	T4330	T4329	compound	Figure,2A
R2858	T4331	T4329	punct	),2A
R2859	T4332	T4315	punct	", ",analyzed
R2860	T4333	T4315	cc	and,analyzed
R2861	T4334	T4335	det	the,remainder
R2862	T4335	T4336	nsubjpass	remainder,incubated
R2863	T4336	T4315	conj	incubated,analyzed
R2864	T4337	T4336	auxpass	was,incubated
R2865	T4338	T4336	advmod	further,incubated
R2866	T4339	T4336	prep	in,incubated
R2867	T4340	T4341	det	the,presence
R2868	T4341	T4339	pobj	presence,in
R2869	T4342	T4341	prep	of,presence
R2870	T4343	T4344	nummod	10,mM
R2871	T4344	T4345	compound	mM,ATP
R2872	T4345	T4342	pobj	ATP,of
R2873	T4346	T4347	aux	to,measure
R2874	T4347	T4336	advcl	measure,incubated
R2875	T4348	T4349	compound	DNA,helicase
R2876	T4349	T4350	compound	helicase,activity
R2877	T4350	T4347	dobj	activity,measure
R2878	T4351	T4352	punct	(,2B
R2879	T4352	T4347	parataxis	2B,measure
R2880	T4353	T4352	compound	Figure,2B
R2881	T4354	T4352	punct	),2B
R2882	T4355	T4336	punct	.,incubated
R2883	T4357	T4358	nsubj	Bub,generated
R2884	T4359	T4357	punct	-,Bub
R2885	T4360	T4357	nummod	10,Bub
R2886	T4361	T4362	advmod	only,level
R2887	T4362	T4358	dobj	level,generated
R2888	T4363	T4362	det	a,level
R2889	T4364	T4362	amod	low,level
R2890	T4365	T4362	prep	of,level
R2891	T4366	T4365	pobj	complexes,of
R2892	T4367	T4366	prep	with,complexes
R2893	T4368	T4367	pobj	Mcm4,with
R2894	T4369	T4368	punct	/,Mcm4
R2895	T4370	T4368	nummod	6,Mcm4
R2896	T4371	T4368	punct	/,Mcm4
R2897	T4372	T4368	nummod	7,Mcm4
R2898	T4373	T4358	punct	", ",generated
R2899	T4374	T4358	cc	and,generated
R2900	T4375	T4376	nsubj	Bub,generated
R2901	T4376	T4358	conj	generated,generated
R2902	T4377	T4375	punct	-,Bub
R2903	T4378	T4375	nummod	20,Bub
R2904	T4379	T4376	advmod	primarily,generated
R2905	T4380	T4381	det	a,form
R2906	T4381	T4376	dobj	form,generated
R2907	T4382	T4381	amod	single,form
R2908	T4383	T4384	npadvmod	mobility,shifted
R2909	T4384	T4381	amod	shifted,form
R2910	T4385	T4384	punct	-,shifted
R2911	T4386	T4381	punct	", ",form
R2912	T4387	T4388	dep	which,contain
R2913	T4388	T4381	relcl	contain,form
R2914	T4389	T4388	aux	may,contain
R2915	T4390	T4391	det	a,hexamer
R2916	T4391	T4388	dobj	hexamer,contain
R2917	T4392	T4391	amod	single,hexamer
R2918	T4393	T4391	prep	of,hexamer
R2919	T4394	T4393	pobj	Mcm4,of
R2920	T4395	T4394	punct	/,Mcm4
R2921	T4396	T4394	nummod	6,Mcm4
R2922	T4397	T4394	punct	/,Mcm4
R2923	T4398	T4394	nummod	7,Mcm4
R2924	T4399	T4376	punct	.,generated
R2925	T4401	T4402	prep	On,generated
R2926	T4403	T4401	pobj	Bub,On
R2927	T4404	T4403	punct	-,Bub
R2928	T4405	T4403	nummod	30,Bub
R2929	T4406	T4402	punct	", ",generated
R2930	T4407	T4408	amod	significant,amount
R2931	T4408	T4402	nsubjpass	amount,generated
R2932	T4409	T4408	prep	of,amount
R2933	T4410	T4409	pobj	complexes,of
R2934	T4411	T4410	acl	containing,complexes
R2935	T4412	T4413	det	a,hexamer
R2936	T4413	T4411	dobj	hexamer,containing
R2937	T4414	T4413	amod	double,hexamer
R2938	T4415	T4402	auxpass	were,generated
R2939	T4416	T4402	prep	in,generated
R2940	T4417	T4416	pobj	addition,in
R2941	T4418	T4417	prep	to,addition
R2942	T4419	T4418	pobj	those,to
R2943	T4420	T4419	acl	containing,those
R2944	T4421	T4422	det	a,hexamer
R2945	T4422	T4420	dobj	hexamer,containing
R2946	T4423	T4422	amod	single,hexamer
R2947	T4424	T4425	punct	(,2A
R2948	T4425	T4402	parataxis	2A,generated
R2949	T4426	T4425	compound	Figure,2A
R2950	T4427	T4425	punct	),2A
R2951	T4428	T4429	punct	(,21
R2952	T4429	T4402	parataxis	21,generated
R2953	T4430	T4429	nummod	13,21
R2954	T4431	T4429	punct	",",21
R2955	T4432	T4429	punct	),21
R2956	T4433	T4402	punct	.,generated
R2957	T4435	T4436	det	The,amounts
R2958	T4436	T4437	nsubj	amounts,increased
R2959	T4438	T4436	prep	of,amounts
R2960	T4439	T4440	amod	double,hexamer
R2961	T4440	T4441	compound	hexamer,complexes
R2962	T4441	T4438	pobj	complexes,of
R2963	T4442	T4437	prep	on,increased
R2964	T4443	T4444	compound	Bub,T40
R2965	T4444	T4442	pobj	T40,on
R2966	T4445	T4444	punct	-,T40
R2967	T4446	T4444	cc	and,T40
R2968	T4447	T4448	compound	Bub,T50
R2969	T4448	T4444	conj	T50,T40
R2970	T4449	T4448	punct	-,T50
R2971	T4450	T4437	prep	with,increased
R2972	T4451	T4452	amod	concomitant,decrease
R2973	T4452	T4450	pobj	decrease,with
R2974	T4453	T4452	prep	of,decrease
R2975	T4454	T4455	amod	single,hexamer
R2976	T4455	T4456	compound	hexamer,complexes
R2977	T4456	T4453	pobj	complexes,of
R2978	T4457	T4458	punct	(,2A
R2979	T4458	T4437	parataxis	2A,increased
R2980	T4459	T4458	compound	Figure,2A
R2981	T4460	T4458	punct	),2A
R2982	T4461	T4437	punct	.,increased
R2983	T4463	T4464	det	The,results
R2984	T4464	T4465	nsubj	results,indicate
R2985	T4466	T4467	mark	that,required
R2986	T4467	T4465	ccomp	required,indicate
R2987	T4468	T4469	det	a,segment
R2988	T4469	T4467	nsubjpass	segment,required
R2989	T4470	T4471	amod	single,stranded
R2990	T4471	T4469	amod	stranded,segment
R2991	T4472	T4471	punct	-,stranded
R2992	T4473	T4469	prep	of,segment
R2993	T4474	T4475	advmod	at,least
R2994	T4475	T4476	advmod	least,40
R2995	T4476	T4477	nummod	40,nt
R2996	T4477	T4473	pobj	nt,of
R2997	T4478	T4477	amod	long,nt
R2998	T4479	T4467	auxpass	is,required
R2999	T4480	T4467	prep	for,required
R3000	T4481	T4482	amod	efficient,loading
R3001	T4482	T4480	pobj	loading,for
R3002	T4483	T4482	prep	of,loading
R3003	T4484	T4485	det	the,helicase
R3004	T4485	T4483	pobj	helicase,of
R3005	T4486	T4487	amod	double,hexameric
R3006	T4487	T4485	amod	hexameric,helicase
R3007	T4488	T4487	punct	-,hexameric
R3008	T4489	T4485	compound	Mcm,helicase
R3009	T4490	T4482	prep	on,loading
R3010	T4491	T4492	det	a,substrate
R3011	T4492	T4490	pobj	substrate,on
R3012	T4493	T4492	compound	bubble,substrate
R3013	T4494	T4465	punct	.,indicate
R3014	T4496	T4497	prep	In,show
R3015	T4498	T4499	compound	DNA,helicase
R3016	T4499	T4500	compound	helicase,assays
R3017	T4500	T4496	pobj	assays,In
R3018	T4501	T4497	punct	", ",show
R3019	T4502	T4503	nmod	Mcm4,complex
R3020	T4503	T4497	nsubj	complex,show
R3021	T4504	T4502	punct	/,Mcm4
R3022	T4505	T4502	nummod	6,Mcm4
R3023	T4506	T4502	punct	/,Mcm4
R3024	T4507	T4502	nummod	7,Mcm4
R3025	T4508	T4497	aux	did,show
R3026	T4509	T4497	neg	not,show
R3027	T4510	T4511	compound	helicase,activity
R3028	T4511	T4497	dobj	activity,show
R3029	T4512	T4497	prep	on,show
R3030	T4513	T4514	preconj	either,Bub
R3031	T4514	T4515	nmod	Bub,substrate
R3032	T4515	T4512	pobj	substrate,on
R3033	T4516	T4514	punct	-,Bub
R3034	T4517	T4514	nummod	10,Bub
R3035	T4518	T4514	cc	or,Bub
R3036	T4519	T4514	conj	Bub,Bub
R3037	T4520	T4519	punct	-,Bub
R3038	T4521	T4519	nummod	20,Bub
R3039	T4522	T4497	punct	.,show
R3040	T4524	T4525	det	The,efficiency
R3041	T4525	T4526	nsubj	efficiency,improved
R3042	T4527	T4525	prep	of,efficiency
R3043	T4528	T4527	pobj	displacement,of
R3044	T4529	T4526	advmod	slightly,improved
R3045	T4530	T4531	mark	as,increased
R3046	T4531	T4526	advcl	increased,improved
R3047	T4532	T4533	det	the,length
R3048	T4533	T4531	nsubj	length,increased
R3049	T4534	T4533	prep	of,length
R3050	T4535	T4536	det	the,segment
R3051	T4536	T4534	pobj	segment,of
R3052	T4537	T4538	amod	single,stranded
R3053	T4538	T4536	amod	stranded,segment
R3054	T4539	T4538	punct	-,stranded
R3055	T4540	T4541	quantmod	up,30
R3056	T4541	T4543	nummod	30,nt
R3057	T4542	T4541	quantmod	to,30
R3058	T4543	T4531	npadvmod	nt,increased
R3059	T4544	T4526	punct	.,improved
R3060	T4546	T4547	prep	On,observed
R3061	T4548	T4549	compound	Bub,T40
R3062	T4549	T4546	pobj	T40,On
R3063	T4550	T4549	punct	-,T40
R3064	T4551	T4549	cc	and,T40
R3065	T4552	T4553	compound	Bub,T50
R3066	T4553	T4549	conj	T50,T40
R3067	T4554	T4553	punct	-,T50
R3068	T4555	T4547	punct	", ",observed
R3069	T4556	T4547	prep	on,observed
R3070	T4557	T4558	det	the,hand
R3071	T4558	T4556	pobj	hand,on
R3072	T4559	T4558	amod	other,hand
R3073	T4560	T4547	punct	", ",observed
R3074	T4561	T4562	amod	significant,displacement
R3075	T4562	T4547	nsubjpass	displacement,observed
R3076	T4563	T4547	auxpass	was,observed
R3077	T4564	T4547	punct	", ",observed
R3078	T4565	T4547	advcl	indicating,observed
R3079	T4566	T4567	mark	that,required
R3080	T4567	T4565	ccomp	required,indicating
R3081	T4568	T4569	det	a,segment
R3082	T4569	T4567	nsubjpass	segment,required
R3083	T4570	T4571	amod	single,stranded
R3084	T4571	T4569	amod	stranded,segment
R3085	T4572	T4571	punct	-,stranded
R3086	T4573	T4569	prep	of,segment
R3087	T4574	T4575	advmod	at,least
R3088	T4575	T4576	advmod	least,40
R3089	T4576	T4577	nummod	40,nt
R3090	T4577	T4573	pobj	nt,of
R3091	T4578	T4577	amod	long,nt
R3092	T4579	T4567	auxpass	is,required
R3093	T4580	T4567	prep	for,required
R3094	T4581	T4582	amod	efficient,actions
R3095	T4582	T4580	pobj	actions,for
R3096	T4583	T4582	compound	helicase,actions
R3097	T4584	T4582	prep	on,actions
R3098	T4585	T4586	compound	bubble,substrates
R3099	T4586	T4584	pobj	substrates,on
R3100	T4587	T4588	punct	(,2B
R3101	T4588	T4567	parataxis	2B,required
R3102	T4589	T4588	compound	Figure,2B
R3103	T4590	T4588	punct	),2B
R3104	T4591	T4547	punct	.,observed
R3105	T4593	T4594	advcl	Taken,are
R3106	T4595	T4593	advmod	together,Taken
R3107	T4596	T4594	punct	", ",are
R3108	T4597	T4598	det	the,results
R3109	T4598	T4594	nsubj	results,are
R3110	T4599	T4598	amod	above,results
R3111	T4600	T4594	acomp	consistent,are
R3112	T4601	T4600	prep	with,consistent
R3113	T4602	T4603	det	the,notion
R3114	T4603	T4601	pobj	notion,with
R3115	T4604	T4605	mark	that,unwinds
R3116	T4605	T4603	acl	unwinds,notion
R3117	T4606	T4607	det	the,complex
R3118	T4607	T4605	nsubj	complex,unwinds
R3119	T4608	T4607	nmod	Mcm4,complex
R3120	T4609	T4608	punct	/,Mcm4
R3121	T4610	T4608	nummod	6,Mcm4
R3122	T4611	T4608	punct	/,Mcm4
R3123	T4612	T4608	nummod	7,Mcm4
R3124	T4613	T4607	punct	", ",complex
R3125	T4614	T4607	acl	loaded,complex
R3126	T4615	T4614	prep	onto,loaded
R3127	T4616	T4615	pobj	DNA,onto
R3128	T4617	T4614	prep	as,loaded
R3129	T4618	T4619	det	a,complex
R3130	T4619	T4617	pobj	complex,as
R3131	T4620	T4621	amod	double,hexameric
R3132	T4621	T4619	amod	hexameric,complex
R3133	T4622	T4605	punct	", ",unwinds
R3134	T4623	T4605	advmod	efficiently,unwinds
R3135	T4624	T4625	det	the,region
R3136	T4625	T4605	dobj	region,unwinds
R3137	T4626	T4625	compound	duplex,region
R3138	T4627	T4625	prep	of,region
R3139	T4628	T4629	det	the,DNA
R3140	T4629	T4627	pobj	DNA,of
R3141	T4630	T4629	compound	substrate,DNA
R3142	T4631	T4594	punct	.,are
R3145	T4737	T4738	det	Both,strands
R3146	T4738	T4739	nsubjpass	strands,required
R3147	T4740	T4738	prep	of,strands
R3148	T4741	T4742	npadvmod	thymine,rich
R3149	T4742	T4744	amod	rich,segments
R3150	T4743	T4742	punct	-,rich
R3151	T4744	T4740	pobj	segments,of
R3152	T4745	T4746	amod	single,stranded
R3153	T4746	T4744	amod	stranded,segments
R3154	T4747	T4746	punct	-,stranded
R3155	T4748	T4739	auxpass	are,required
R3156	T4749	T4739	prep	for,required
R3157	T4750	T4751	amod	efficient,unwinding
R3158	T4751	T4749	pobj	unwinding,for
R3159	T4752	T4751	prep	of,unwinding
R3160	T4753	T4754	compound	bubble,DNA
R3161	T4754	T4752	pobj	DNA,of
R3162	T4755	T4751	prep	by,unwinding
R3163	T4756	T4755	pobj	Mcm4,by
R3164	T4757	T4756	punct	/,Mcm4
R3165	T4758	T4756	nummod	6,Mcm4
R3166	T4759	T4756	punct	/,Mcm4
R3167	T4760	T4756	nummod	7,Mcm4
R3168	T4762	T4763	amod	Symmetric,binding
R3169	T4763	T4764	nsubj	binding,suggests
R3170	T4765	T4763	prep	of,binding
R3171	T4766	T4767	det	the,hexamer
R3172	T4767	T4765	pobj	hexamer,of
R3173	T4768	T4767	nmod	Mcm4,hexamer
R3174	T4769	T4768	punct	/,Mcm4
R3175	T4770	T4768	nummod	6,Mcm4
R3176	T4771	T4768	punct	/,Mcm4
R3177	T4772	T4768	nummod	7,Mcm4
R3178	T4773	T4767	amod	double,hexamer
R3179	T4774	T4763	prep	to,binding
R3180	T4775	T4774	pobj	bubble,to
R3181	T4776	T4777	mark	that,catalyzes
R3182	T4777	T4764	ccomp	catalyzes,suggests
R3183	T4778	T4779	det	each,hexamer
R3184	T4779	T4777	nsubj	hexamer,catalyzes
R3185	T4780	T4777	dobj	unwinding,catalyzes
R3186	T4781	T4780	prep	of,unwinding
R3187	T4782	T4781	pobj	one,of
R3188	T4783	T4782	prep	of,one
R3189	T4784	T4785	det	the,segments
R3190	T4785	T4783	pobj	segments,of
R3191	T4786	T4785	nummod	two,segments
R3192	T4787	T4785	compound	duplex,segments
R3193	T4788	T4764	punct	.,suggests
R3194	T4790	T4791	nsubj	We,asked
R3195	T4792	T4793	mark	whether,required
R3196	T4793	T4791	ccomp	required,asked
R3197	T4794	T4793	nsubjpass	activation,required
R3198	T4795	T4794	prep	of,activation
R3199	T4796	T4797	det	both,hexamers
R3200	T4797	T4795	pobj	hexamers,of
R3201	T4798	T4793	auxpass	is,required
R3202	T4799	T4793	prep	for,required
R3203	T4800	T4799	pobj	unwinding,for
R3204	T4801	T4800	prep	of,unwinding
R3205	T4802	T4803	det	the,substrate
R3206	T4803	T4801	pobj	substrate,of
R3207	T4804	T4803	compound	bubble,substrate
R3208	T4805	T4791	punct	.,asked
R3209	T4807	T4808	det	The,substrate
R3210	T4808	T4811	nsubj	substrate,contains
R3211	T4809	T4808	amod	preferred,substrate
R3212	T4810	T4808	compound	bubble,substrate
R3213	T4812	T4813	dep	which,unwound
R3214	T4813	T4808	relcl	unwound,substrate
R3215	T4814	T4813	auxpass	is,unwound
R3216	T4815	T4813	advmod	efficiently,unwound
R3217	T4816	T4813	agent	by,unwound
R3218	T4817	T4816	pobj	Mcm4,by
R3219	T4818	T4817	punct	/,Mcm4
R3220	T4819	T4817	nummod	6,Mcm4
R3221	T4820	T4817	punct	/,Mcm4
R3222	T4821	T4817	nummod	7,Mcm4
R3223	T4822	T4823	compound	thymine,stretches
R3224	T4823	T4811	dobj	stretches,contains
R3225	T4824	T4823	prep	on,stretches
R3226	T4825	T4826	det	both,strands
R3227	T4826	T4824	pobj	strands,on
R3228	T4827	T4826	prep	of,strands
R3229	T4828	T4829	det	the,segment
R3230	T4829	T4827	pobj	segment,of
R3231	T4830	T4831	amod	single,stranded
R3232	T4831	T4829	amod	stranded,segment
R3233	T4832	T4831	punct	-,stranded
R3234	T4833	T4811	punct	.,contains
R3235	T4835	T4836	nsubj	We,constructed
R3236	T4837	T4838	det	a,hetero-bubble
R3237	T4838	T4836	dobj	hetero-bubble,constructed
R3238	T4839	T4838	punct	(,hetero-bubble
R3239	T4840	T4841	compound	Bub66,G
R3240	T4841	T4838	appos	G,hetero-bubble
R3241	T4842	T4841	punct	/,G
R3242	T4843	T4841	compound	T,G
R3243	T4844	T4841	punct	–,G
R3244	T4845	T4841	punct	-,G
R3245	T4846	T4841	amod	rich,G
R3246	T4847	T4838	punct	),hetero-bubble
R3247	T4848	T4849	prep	in,is
R3248	T4849	T4838	relcl	is,hetero-bubble
R3249	T4850	T4848	pobj	which,in
R3250	T4851	T4852	nummod	one,strand
R3251	T4852	T4849	nsubj	strand,is
R3252	T4853	T4854	npadvmod	T,rich
R3253	T4854	T4849	acomp	rich,is
R3254	T4855	T4854	punct	-,rich
R3255	T4856	T4857	punct	(,same
R3256	T4857	T4854	parataxis	same,rich
R3257	T4858	T4857	prep	as,same
R3258	T4859	T4860	compound	Bub66,T
R3259	T4860	T4858	pobj	T,as
R3260	T4861	T4860	punct	/,T
R3261	T4862	T4860	punct	-,T
R3262	T4863	T4860	amod	rich,T
R3263	T4864	T4857	punct	),same
R3264	T4865	T4849	cc	and,is
R3265	T4866	T4867	det	the,strand
R3266	T4867	T4869	nsubj	strand,is
R3267	T4868	T4867	amod	other,strand
R3268	T4869	T4849	conj	is,is
R3269	T4870	T4871	npadvmod	G,rich
R3270	T4871	T4869	acomp	rich,is
R3271	T4872	T4871	punct	-,rich
R3272	T4873	T4874	punct	(,same
R3273	T4874	T4871	parataxis	same,rich
R3274	T4875	T4874	prep	as,same
R3275	T4876	T4877	compound	Bub66,G
R3276	T4877	T4875	pobj	G,as
R3277	T4878	T4877	punct	/,G
R3278	T4879	T4877	punct	-,G
R3279	T4880	T4877	amod	rich,G
R3280	T4881	T4874	punct	),same
R3281	T4882	T4883	punct	(,13
R3282	T4883	T4871	parataxis	13,rich
R3283	T4884	T4883	punct	),13
R3284	T4885	T4836	punct	", ",constructed
R3285	T4886	T4836	cc	and,constructed
R3286	T4887	T4888	compound	helicase,assays
R3287	T4888	T4889	nsubjpass	assays,conducted
R3288	T4889	T4836	conj	conducted,constructed
R3289	T4890	T4889	auxpass	were,conducted
R3290	T4891	T4889	prep	with,conducted
R3291	T4892	T4893	amod	increasing,amount
R3292	T4893	T4891	pobj	amount,with
R3293	T4894	T4893	prep	of,amount
R3294	T4895	T4894	pobj	Mcm4,of
R3295	T4896	T4895	punct	/,Mcm4
R3296	T4897	T4895	nummod	6,Mcm4
R3297	T4898	T4895	punct	/,Mcm4
R3298	T4899	T4895	nummod	7,Mcm4
R3299	T4900	T4889	punct	.,conducted
R3300	T4902	T4903	advmod	Only,unwinding
R3301	T4903	T4905	nsubjpass	unwinding,detected
R3302	T4904	T4903	amod	weak,unwinding
R3303	T4906	T4905	auxpass	was,detected
R3304	T4907	T4905	prep	on,detected
R3305	T4908	T4909	det	this,substrate
R3306	T4909	T4907	pobj	substrate,on
R3307	T4910	T4905	prep	compared,detected
R3308	T4911	T4910	prep	to,compared
R3309	T4912	T4913	compound	Bub66,T
R3310	T4913	T4911	pobj	T,to
R3311	T4914	T4913	punct	/,T
R3312	T4915	T4913	punct	-,T
R3313	T4916	T4913	amod	rich,T
R3314	T4917	T4918	punct	(,3A
R3315	T4918	T4905	parataxis	3A,detected
R3316	T4919	T4918	compound	Figure,3A
R3317	T4920	T4918	punct	),3A
R3318	T4921	T4905	punct	.,detected
R3319	T4923	T4924	mark	Since,bind
R3320	T4924	T4931	advcl	bind,is
R3321	T4925	T4924	nsubj	Mcm4,bind
R3322	T4926	T4925	punct	/,Mcm4
R3323	T4927	T4925	nummod	6,Mcm4
R3324	T4928	T4925	punct	/,Mcm4
R3325	T4929	T4925	nummod	7,Mcm4
R3326	T4930	T4924	aux	can,bind
R3327	T4932	T4924	prep	to,bind
R3328	T4933	T4934	compound	Bub66,G
R3329	T4934	T4932	pobj	G,to
R3330	T4935	T4934	punct	/,G
R3331	T4936	T4934	compound	T,G
R3332	T4937	T4934	punct	–,G
R3333	T4938	T4934	punct	-,G
R3334	T4939	T4934	amod	rich,G
R3335	T4940	T4924	cc	and,bind
R3336	T4941	T4924	conj	forms,bind
R3337	T4942	T4943	det	a,complex
R3338	T4943	T4941	dobj	complex,forms
R3339	T4944	T4945	amod	double,hexameric
R3340	T4945	T4943	amod	hexameric,complex
R3341	T4946	T4947	punct	(,shown
R3342	T4947	T4941	parataxis	shown,forms
R3343	T4948	T4947	nsubj	data,shown
R3344	T4949	T4947	neg	not,shown
R3345	T4950	T4947	punct	),shown
R3346	T4951	T4931	punct	", ",is
R3347	T4952	T4953	det	the,unwinding
R3348	T4953	T4931	nsubj	unwinding,is
R3349	T4954	T4953	amod	defective,unwinding
R3350	T4955	T4931	neg	not,is
R3351	T4956	T4931	prep	due,is
R3352	T4957	T4956	pcomp	to,due
R3353	T4958	T4959	amod	inefficient,loading
R3354	T4959	T4956	pobj	loading,due
R3355	T4960	T4959	prep	of,loading
R3356	T4961	T4962	det	the,helicase
R3357	T4962	T4960	pobj	helicase,of
R3358	T4963	T4959	prep	onto,loading
R3359	T4964	T4965	det	the,substrate
R3360	T4965	T4963	pobj	substrate,onto
R3361	T4966	T4931	punct	.,is
R3362	T4968	T4969	mark	If,dissociates
R3363	T4969	T4979	advcl	dissociates,result
R3364	T4970	T4971	nummod	one,hexamer
R3365	T4971	T4969	nsubj	hexamer,dissociates
R3366	T4972	T4971	acl	interacting,hexamer
R3367	T4973	T4972	prep	with,interacting
R3368	T4974	T4975	det	the,strand
R3369	T4975	T4973	pobj	strand,with
R3370	T4976	T4977	npadvmod	thymine,rich
R3371	T4977	T4975	amod	rich,strand
R3372	T4978	T4977	punct	-,rich
R3373	T4980	T4969	prep	from,dissociates
R3374	T4981	T4982	det	the,hexamer
R3375	T4982	T4980	pobj	hexamer,from
R3376	T4983	T4982	amod	other,hexamer
R3377	T4984	T4969	cc	and,dissociates
R3378	T4985	T4969	conj	displaces,dissociates
R3379	T4986	T4987	advmod	only,one
R3380	T4987	T4985	dobj	one,displaces
R3381	T4988	T4987	prep	of,one
R3382	T4989	T4990	det	the,segments
R3383	T4990	T4988	pobj	segments,of
R3384	T4991	T4990	compound	duplex,segments
R3385	T4992	T4979	punct	", ",result
R3386	T4993	T4979	advmod	then,result
R3387	T4994	T4979	nsubj	it,result
R3388	T4995	T4979	aux	would,result
R3389	T4996	T4979	prep	in,result
R3390	T4997	T4996	pobj	generation,in
R3391	T4998	T4997	prep	of,generation
R3392	T4999	T5000	compound	Y,fork
R3393	T5000	T5002	compound	fork,structures
R3394	T5001	T5000	punct	-,fork
R3395	T5002	T4998	pobj	structures,of
R3396	T5003	T5004	dep	which,displaced
R3397	T5004	T5002	relcl	displaced,structures
R3398	T5005	T5004	aux	could,displaced
R3399	T5006	T5004	neg	not,displaced
R3400	T5007	T5004	auxpass	be,displaced
R3401	T5008	T5004	advmod	further,displaced
R3402	T5009	T5004	prep	due,displaced
R3403	T5010	T5009	pcomp	to,due
R3404	T5011	T5012	det	the,presence
R3405	T5012	T5009	pobj	presence,due
R3406	T5013	T5012	prep	of,presence
R3407	T5014	T5015	npadvmod	G,rich
R3408	T5015	T5017	amod	rich,tail
R3409	T5016	T5015	punct	-,rich
R3410	T5017	T5013	pobj	tail,of
R3411	T5018	T5017	nummod	3,tail
R3412	T5019	T5018	punct	′,3
R3413	T5020	T5017	punct	-,tail
R3414	T5021	T5004	cc	and,displaced
R3415	T5022	T5023	aux	would,appear
R3416	T5023	T5004	conj	appear,displaced
R3417	T5024	T5023	prep	on,appear
R3418	T5025	T5026	det	the,gel
R3419	T5026	T5024	pobj	gel,on
R3420	T5027	T4979	punct	.,result
R3421	T5029	T5030	advmod	However,detect
R3422	T5031	T5030	punct	", ",detect
R3423	T5032	T5030	nsubj	we,detect
R3424	T5033	T5030	aux	did,detect
R3425	T5034	T5030	neg	not,detect
R3426	T5035	T5036	amod	such,intermediates
R3427	T5036	T5030	dobj	intermediates,detect
R3428	T5037	T5030	prep	during,detect
R3429	T5038	T5039	det	the,course
R3430	T5039	T5037	pobj	course,during
R3431	T5040	T5039	prep	of,course
R3432	T5041	T5042	det	the,experiment
R3433	T5042	T5040	pobj	experiment,of
R3434	T5043	T5044	punct	(,3B
R3435	T5044	T5030	parataxis	3B,detect
R3436	T5045	T5044	compound	Figure,3B
R3437	T5046	T5044	punct	),3B
R3438	T5047	T5030	punct	", ",detect
R3439	T5048	T5030	advcl	suggesting,detect
R3440	T5049	T5050	mark	that,displaced
R3441	T5050	T5048	ccomp	displaced,suggesting
R3442	T5051	T5050	nsubjpass	bubble,displaced
R3443	T5052	T5050	aux	can,displaced
R3444	T5053	T5050	auxpass	be,displaced
R3445	T5054	T5050	advmod	efficiently,displaced
R3446	T5055	T5056	advmod	only,activated
R3447	T5056	T5050	advcl	activated,displaced
R3448	T5057	T5056	advmod	when,activated
R3449	T5058	T5059	nummod	two,molecules
R3450	T5059	T5056	nsubjpass	molecules,activated
R3451	T5060	T5059	prep	of,molecules
R3452	T5061	T5062	det	the,hexamers
R3453	T5062	T5060	pobj	hexamers,of
R3454	T5063	T5062	nmod	Mcm4,hexamers
R3455	T5064	T5063	punct	/,Mcm4
R3456	T5065	T5063	nummod	6,Mcm4
R3457	T5066	T5063	punct	/,Mcm4
R3458	T5067	T5063	nummod	7,Mcm4
R3459	T5068	T5056	auxpass	are,activated
R3460	T5069	T5056	advmod	simultaneously,activated
R3461	T5070	T5030	punct	.,detect
R3464	T5260	T5261	nmod	Binding,actions
R3465	T5262	T5260	cc	and,Binding
R3466	T5263	T5260	conj	helicase,Binding
R3467	T5264	T5261	prep	of,actions
R3468	T5265	T5264	pobj	Mcm4,of
R3469	T5266	T5265	punct	/,Mcm4
R3470	T5267	T5265	nummod	6,Mcm4
R3471	T5268	T5265	punct	/,Mcm4
R3472	T5269	T5265	nummod	7,Mcm4
R3473	T5270	T5261	prep	on,actions
R3474	T5271	T5272	amod	forked,substrates
R3475	T5272	T5270	pobj	substrates,on
R3476	T5273	T5271	cc	and,forked
R3477	T5274	T5275	nummod	3,extension
R3478	T5275	T5271	conj	extension,forked
R3479	T5276	T5274	punct	′,3
R3480	T5277	T5274	punct	-,3
R3481	T5278	T5274	cc	or,3
R3482	T5279	T5274	conj	5,3
R3483	T5280	T5279	punct	′,5
R3484	T5281	T5275	punct	-,extension
R3485	T5283	T5284	prep	In,constructed
R3486	T5285	T5283	pobj	order,In
R3487	T5286	T5287	aux	to,examine
R3488	T5287	T5285	acl	examine,order
R3489	T5288	T5289	advmod	more,precisely
R3490	T5289	T5287	advmod	precisely,examine
R3491	T5290	T5291	det	the,interaction
R3492	T5291	T5287	dobj	interaction,examine
R3493	T5292	T5291	cc	and,interaction
R3494	T5293	T5294	compound	helicase,actions
R3495	T5294	T5291	conj	actions,interaction
R3496	T5295	T5291	prep	with,interaction
R3497	T5296	T5297	amod	forked,substrates
R3498	T5297	T5295	pobj	substrates,with
R3499	T5298	T5284	punct	", ",constructed
R3500	T5299	T5300	compound	Y,fork
R3501	T5300	T5302	compound	fork,substrates
R3502	T5301	T5300	punct	-,fork
R3503	T5302	T5284	nsubjpass	substrates,constructed
R3504	T5303	T5302	acl	containing,substrates
R3505	T5304	T5305	det	a,strand
R3506	T5305	T5303	dobj	strand,containing
R3507	T5306	T5305	amod	nascent,strand
R3508	T5307	T5305	amod	leading,strand
R3509	T5308	T5305	punct	(,strand
R3510	T5309	T5310	compound	A,fork
R3511	T5310	T5305	appos	fork,strand
R3512	T5311	T5310	punct	-,fork
R3513	T5312	T5310	punct	[,fork
R3514	T5313	T5310	nummod	3,fork
R3515	T5314	T5310	punct	′,fork
R3516	T5315	T5305	punct	],strand
R3517	T5316	T5305	punct	),strand
R3518	T5317	T5305	punct	", ",strand
R3519	T5318	T5319	amod	nascent,strand
R3520	T5319	T5305	appos	strand,strand
R3521	T5320	T5319	amod	lagging,strand
R3522	T5321	T5319	punct	(,strand
R3523	T5322	T5323	compound	A,fork
R3524	T5323	T5319	appos	fork,strand
R3525	T5324	T5323	punct	-,fork
R3526	T5325	T5323	punct	[,fork
R3527	T5326	T5323	nummod	5,fork
R3528	T5327	T5323	punct	′,fork
R3529	T5328	T5305	punct	],strand
R3530	T5329	T5305	punct	),strand
R3531	T5330	T5305	punct	", ",strand
R3532	T5331	T5305	cc	or,strand
R3533	T5332	T5305	conj	both,strand
R3534	T5333	T5332	prep	of,both
R3535	T5334	T5333	pobj	them,of
R3536	T5335	T5332	punct	(,both
R3537	T5336	T5337	nmod	A,fork
R3538	T5337	T5339	nmod	fork,structures
R3539	T5338	T5337	punct	-,fork
R3540	T5339	T5332	appos	structures,both
R3541	T5340	T5337	punct	[,fork
R3542	T5341	T5337	appos	3,fork
R3543	T5342	T5341	punct	′,3
R3544	T5343	T5341	punct	",",3
R3545	T5344	T5341	appos	5,3
R3546	T5345	T5344	punct	′,5
R3547	T5346	T5337	punct	],fork
R3548	T5347	T5337	punct	),fork
R3549	T5348	T5337	punct	", ",fork
R3550	T5349	T5350	advmod	as,as
R3551	T5350	T5337	cc	as,fork
R3552	T5351	T5350	advmod	well,as
R3553	T5352	T5353	nummod	5,extension
R3554	T5353	T5337	conj	extension,fork
R3555	T5354	T5352	punct	′,5
R3556	T5355	T5353	punct	-,extension
R3557	T5356	T5353	cc	or,extension
R3558	T5357	T5358	nummod	3,extension
R3559	T5358	T5353	conj	extension,extension
R3560	T5359	T5357	punct	′,3
R3561	T5360	T5358	punct	-,extension
R3562	T5361	T5284	auxpass	were,constructed
R3563	T5362	T5284	punct	.,constructed
R3564	T5364	T5365	nsubj	Mcm4,bound
R3565	T5366	T5364	punct	/,Mcm4
R3566	T5367	T5364	nummod	6,Mcm4
R3567	T5368	T5364	punct	/,Mcm4
R3568	T5369	T5364	nummod	7,Mcm4
R3569	T5370	T5365	prep	to,bound
R3570	T5371	T5372	compound	A,fork
R3571	T5372	T5370	pobj	fork,to
R3572	T5373	T5372	punct	-,fork
R3573	T5374	T5372	punct	[,fork
R3574	T5375	T5372	nummod	3,fork
R3575	T5376	T5372	punct	′,fork
R3576	T5377	T5372	punct	],fork
R3577	T5378	T5372	cc	or,fork
R3578	T5379	T5380	compound	A,fork
R3579	T5380	T5372	conj	fork,fork
R3580	T5381	T5380	punct	-,fork
R3581	T5382	T5380	punct	[,fork
R3582	T5383	T5380	nummod	5,fork
R3583	T5384	T5380	punct	′,fork
R3584	T5385	T5365	punct	],bound
R3585	T5386	T5365	prep	with,bound
R3586	T5387	T5386	pobj	affinity,with
R3587	T5388	T5387	amod	similar,affinity
R3588	T5389	T5388	prep	to,similar
R3589	T5390	T5389	pobj	that,to
R3590	T5391	T5390	prep	of,that
R3591	T5392	T5393	det	a,fork
R3592	T5393	T5391	pobj	fork,of
R3593	T5394	T5393	amod	simple,fork
R3594	T5395	T5393	compound	Y,fork
R3595	T5396	T5393	punct	-,fork
R3596	T5397	T5398	punct	(,4A
R3597	T5398	T5365	parataxis	4A,bound
R3598	T5399	T5398	compound	Figure,4A
R3599	T5400	T5398	cc	and,4A
R3600	T5401	T5402	nsubj	data,shown
R3601	T5402	T5398	conj	shown,4A
R3602	T5403	T5402	neg	not,shown
R3603	T5404	T5398	punct	),4A
R3604	T5405	T5365	punct	.,bound
R3605	T5407	T5408	prep	In,bound
R3606	T5409	T5407	pobj	contrast,In
R3607	T5410	T5408	punct	", ",bound
R3608	T5411	T5412	compound	PriA,helicase
R3609	T5412	T5408	nsubj	helicase,bound
R3610	T5413	T5412	punct	", ",helicase
R3611	T5414	T5412	acl	used,helicase
R3612	T5415	T5414	prep	as,used
R3613	T5416	T5417	det	a,control
R3614	T5417	T5415	pobj	control,as
R3615	T5418	T5408	punct	", ",bound
R3616	T5419	T5408	prep	to,bound
R3617	T5420	T5421	compound	A,fork
R3618	T5421	T5419	pobj	fork,to
R3619	T5422	T5421	punct	-,fork
R3620	T5423	T5421	punct	[,fork
R3621	T5424	T5421	nummod	3,fork
R3622	T5425	T5421	punct	′,fork
R3623	T5426	T5408	punct	],bound
R3624	T5427	T5428	advmod	more,efficiently
R3625	T5428	T5408	advmod	efficiently,bound
R3626	T5429	T5428	prep	than,efficiently
R3627	T5430	T5429	prep	to,than
R3628	T5431	T5432	compound	A,fork
R3629	T5432	T5430	pobj	fork,to
R3630	T5433	T5432	punct	-,fork
R3631	T5434	T5432	punct	[,fork
R3632	T5435	T5432	nummod	5,fork
R3633	T5436	T5432	punct	′,fork
R3634	T5437	T5408	punct	],bound
R3635	T5438	T5408	punct	", ",bound
R3636	T5439	T5440	mark	as,reported
R3637	T5440	T5408	advcl	reported,bound
R3638	T5441	T5440	advmod	previously,reported
R3639	T5442	T5443	punct	(,25
R3640	T5443	T5440	parataxis	25,reported
R3641	T5444	T5443	nummod	23,25
R3642	T5445	T5443	punct	",",25
R3643	T5446	T5443	punct	),25
R3644	T5447	T5408	punct	.,bound
R3645	T5449	T5450	nsubj	Mcm4,bound
R3646	T5451	T5449	punct	/,Mcm4
R3647	T5452	T5449	nummod	6,Mcm4
R3648	T5453	T5449	punct	/,Mcm4
R3649	T5454	T5449	nummod	7,Mcm4
R3650	T5455	T5450	advmod	also,bound
R3651	T5456	T5450	prep	to,bound
R3652	T5457	T5458	nummod	5,extension
R3653	T5458	T5456	pobj	extension,to
R3654	T5459	T5457	punct	′,5
R3655	T5460	T5458	punct	-,extension
R3656	T5461	T5458	cc	and,extension
R3657	T5462	T5463	nummod	3,extension
R3658	T5463	T5458	conj	extension,extension
R3659	T5464	T5462	punct	′,3
R3660	T5465	T5463	punct	-,extension
R3661	T5466	T5450	punct	", ",bound
R3662	T5467	T5468	dep	albeit,with
R3663	T5468	T5450	prep	with,bound
R3664	T5469	T5470	advmod	slightly,reduced
R3665	T5470	T5471	amod	reduced,affinity
R3666	T5471	T5468	pobj	affinity,with
R3667	T5472	T5471	prep	compared,affinity
R3668	T5473	T5472	prep	to,compared
R3669	T5474	T5475	compound	A,fork
R3670	T5475	T5473	pobj	fork,to
R3671	T5476	T5475	punct	-,fork
R3672	T5477	T5475	punct	[,fork
R3673	T5478	T5475	nummod	3,fork
R3674	T5479	T5475	punct	′,fork
R3675	T5480	T5475	punct	],fork
R3676	T5481	T5475	cc	and,fork
R3677	T5482	T5483	compound	A,fork
R3678	T5483	T5475	conj	fork,fork
R3679	T5484	T5483	punct	-,fork
R3680	T5485	T5483	punct	[,fork
R3681	T5486	T5483	nummod	5,fork
R3682	T5487	T5483	punct	′,fork
R3683	T5488	T5450	punct	],bound
R3684	T5489	T5490	punct	(,4A
R3685	T5490	T5450	parataxis	4A,bound
R3686	T5491	T5490	compound	Figure,4A
R3687	T5492	T5490	punct	),4A
R3688	T5493	T5450	punct	.,bound
R3689	T5495	T5496	nsubj	Mcm4,bound
R3690	T5497	T5495	punct	/,Mcm4
R3691	T5498	T5495	nummod	6,Mcm4
R3692	T5499	T5495	punct	/,Mcm4
R3693	T5500	T5495	nummod	7,Mcm4
R3694	T5501	T5496	prep	to,bound
R3695	T5502	T5503	compound	A,fork
R3696	T5503	T5501	pobj	fork,to
R3697	T5504	T5503	punct	-,fork
R3698	T5505	T5503	punct	[,fork
R3699	T5506	T5503	appos	3,fork
R3700	T5507	T5506	punct	′,3
R3701	T5508	T5506	punct	",",3
R3702	T5509	T5506	appos	5,3
R3703	T5510	T5509	punct	′,5
R3704	T5511	T5496	punct	],bound
R3705	T5512	T5496	prep	with,bound
R3706	T5513	T5514	advmod	much,lower
R3707	T5514	T5515	amod	lower,affinity
R3708	T5515	T5512	pobj	affinity,with
R3709	T5516	T5496	punct	", ",bound
R3710	T5517	T5496	cc	and,bound
R3711	T5518	T5519	det	the,complex
R3712	T5519	T5520	nsubj	complex,gave
R3713	T5520	T5496	conj	gave,bound
R3714	T5521	T5520	dobj	rise,gave
R3715	T5522	T5520	prep	to,gave
R3716	T5523	T5524	det	a,band
R3717	T5524	T5522	pobj	band,to
R3718	T5525	T5524	amod	single,band
R3719	T5526	T5520	punct	.,gave
R3720	T5528	T5529	det	These,findings
R3721	T5529	T5530	nsubj	findings,indicate
R3722	T5531	T5532	mark	that,loaded
R3723	T5532	T5530	ccomp	loaded,indicate
R3724	T5533	T5534	det	the,complex
R3725	T5534	T5532	nsubjpass	complex,loaded
R3726	T5535	T5534	nmod	Mcm4,complex
R3727	T5536	T5535	punct	/,Mcm4
R3728	T5537	T5535	nummod	6,Mcm4
R3729	T5538	T5535	punct	/,Mcm4
R3730	T5539	T5535	nummod	7,Mcm4
R3731	T5540	T5532	auxpass	is,loaded
R3732	T5541	T5532	prep	onto,loaded
R3733	T5542	T5541	pobj	DNA,onto
R3734	T5543	T5532	advmod	preferentially,loaded
R3735	T5544	T5543	cc	and,preferentially
R3736	T5545	T5543	conj	efficiently,preferentially
R3737	T5546	T5532	prep	through,loaded
R3738	T5547	T5548	amod	single,stranded
R3739	T5548	T5550	amod	stranded,region
R3740	T5549	T5548	punct	-,stranded
R3741	T5550	T5546	pobj	region,through
R3742	T5551	T5532	punct	", ",loaded
R3743	T5552	T5532	advmod	regardless,loaded
R3744	T5553	T5552	prep	of,regardless
R3745	T5554	T5555	det	the,presence
R3746	T5555	T5553	pobj	presence,of
R3747	T5556	T5555	prep	of,presence
R3748	T5557	T5558	nummod	3,terminus
R3749	T5558	T5556	pobj	terminus,of
R3750	T5559	T5557	punct	′,3
R3751	T5560	T5557	punct	-,3
R3752	T5561	T5557	cc	or,3
R3753	T5562	T5557	conj	5,3
R3754	T5563	T5562	punct	′,5
R3755	T5564	T5558	punct	-,terminus
R3756	T5565	T5530	punct	.,indicate
R3757	T5567	T5568	prep	In,unwound
R3758	T5569	T5567	pobj	contrast,In
R3759	T5570	T5568	punct	", ",unwound
R3760	T5571	T5572	advmod	only,substrate
R3761	T5572	T5568	nsubjpass	substrate,unwound
R3762	T5573	T5572	det	the,substrate
R3763	T5574	T5572	acl	containing,substrate
R3764	T5575	T5576	det	a,tail
R3765	T5576	T5574	dobj	tail,containing
R3766	T5577	T5576	nummod	3,tail
R3767	T5578	T5577	punct	′,3
R3768	T5579	T5580	amod	single,stranded
R3769	T5580	T5576	amod	stranded,tail
R3770	T5581	T5580	punct	-,stranded
R3771	T5582	T5576	compound	DNA,tail
R3772	T5583	T5584	punct	(,fork
R3773	T5584	T5572	appos	fork,substrate
R3774	T5585	T5584	compound	A,fork
R3775	T5586	T5584	punct	-,fork
R3776	T5587	T5584	punct	[,fork
R3777	T5588	T5584	nummod	5,fork
R3778	T5589	T5584	punct	′,fork
R3779	T5590	T5568	punct	],unwound
R3780	T5591	T5568	punct	),unwound
R3781	T5592	T5568	auxpass	was,unwound
R3782	T5593	T5568	advmod	efficiently,unwound
R3783	T5594	T5568	cc	and,unwound
R3784	T5595	T5596	compound	A,fork
R3785	T5596	T5598	nsubjpass	fork,unwound
R3786	T5597	T5596	punct	-,fork
R3788	T5599	T5596	punct	[,fork
R3789	T5600	T5596	nummod	3,fork
R3790	T5601	T5596	punct	′,fork
R3791	T5602	T5596	punct	],fork
R3792	T5603	T5596	cc	or,fork
R3793	T5604	T5605	compound	A,fork
R3794	T5605	T5596	conj	fork,fork
R3795	T5606	T5605	punct	-,fork
R3796	T5607	T5605	punct	[,fork
R3797	T5608	T5605	appos	3,fork
R3798	T5609	T5608	punct	′,3
R3799	T5610	T5608	punct	",",3
R3800	T5611	T5608	appos	5,3
R3801	T5612	T5611	punct	′,5
R3802	T5613	T5598	punct	],unwound
R3803	T5614	T5598	punct	", ",unwound
R3804	T5615	T5598	auxpass	was,unwound
R3805	T5616	T5598	neg	not,unwound
R3806	T5617	T5598	agent	by,unwound
R3807	T5618	T5617	pobj	Mcm4,by
R3808	T5619	T5618	punct	/,Mcm4
R3809	T5620	T5618	nummod	6,Mcm4
R3810	T5621	T5618	punct	/,Mcm4
R3811	T5622	T5618	nummod	7,Mcm4
R3812	T5623	T5624	punct	(,4B
R3813	T5624	T5598	parataxis	4B,unwound
R3814	T5625	T5624	compound	Figure,4B
R3815	T5626	T5624	punct	),4B
R3816	T5627	T5568	punct	.,unwound
R3817	T5629	T5630	nsubjpass	Absence,expected
R3818	T5631	T5629	prep	of,Absence
R3819	T5632	T5633	compound	helicase,actions
R3820	T5633	T5631	pobj	actions,of
R3821	T5634	T5629	prep	on,Absence
R3822	T5635	T5636	compound	A,fork
R3823	T5636	T5634	pobj	fork,on
R3824	T5637	T5636	punct	-,fork
R3825	T5638	T5636	punct	[,fork
R3826	T5639	T5636	appos	3,fork
R3827	T5640	T5639	punct	′,3
R3828	T5641	T5639	punct	",",3
R3829	T5642	T5639	appos	5,3
R3830	T5643	T5642	punct	′,5
R3831	T5644	T5636	punct	],fork
R3832	T5645	T5636	acl	composed,fork
R3833	T5646	T5647	advmod	only,of
R3834	T5647	T5645	prep	of,composed
R3835	T5648	T5649	compound	duplex,regions
R3836	T5649	T5647	pobj	regions,of
R3837	T5650	T5630	auxpass	is,expected
R3838	T5651	T5652	mark	since,is
R3839	T5652	T5630	advcl	is,expected
R3840	T5653	T5654	amod	double,stranded
R3841	T5654	T5656	amod	stranded,DNA
R3842	T5655	T5654	punct	-,stranded
R3843	T5656	T5652	nsubj	DNA,is
R3844	T5657	T5652	neg	not,is
R3845	T5658	T5652	acomp	able,is
R3846	T5659	T5660	aux	to,activate
R3847	T5660	T5658	xcomp	activate,able
R3848	T5661	T5662	det	the,activity
R3849	T5662	T5660	dobj	activity,activate
R3850	T5663	T5662	compound	ATPase,activity
R3851	T5664	T5662	prep	of,activity
R3852	T5665	T5664	pobj	Mcm4,of
R3853	T5666	T5665	punct	/,Mcm4
R3854	T5667	T5665	nummod	6,Mcm4
R3855	T5668	T5665	punct	/,Mcm4
R3856	T5669	T5665	nummod	7,Mcm4
R3857	T5670	T5671	punct	(,10
R3858	T5671	T5652	parataxis	10,is
R3859	T5672	T5671	punct	),10
R3860	T5673	T5630	punct	.,expected
R3861	T5675	T5676	prep	In,binds
R3862	T5677	T5675	pobj	contrast,In
R3863	T5678	T5676	punct	", ",binds
R3864	T5679	T5680	det	the,PriA
R3865	T5680	T5676	nsubj	PriA,binds
R3866	T5681	T5680	punct	", ",PriA
R3867	T5682	T5683	det	a,helicase
R3868	T5683	T5680	appos	helicase,PriA
R3869	T5684	T5685	npadvmod	structure,specific
R3870	T5685	T5683	amod	specific,helicase
R3871	T5686	T5685	punct	-,specific
R3872	T5687	T5683	acl	used,helicase
R3873	T5688	T5687	prep	as,used
R3874	T5689	T5690	det	a,control
R3875	T5690	T5688	pobj	control,as
R3876	T5691	T5676	punct	", ",binds
R3877	T5692	T5676	prep	at,binds
R3878	T5693	T5694	det	the,junction
R3879	T5694	T5692	pobj	junction,at
R3880	T5695	T5694	compound	fork,junction
R3881	T5696	T5676	cc	and,binds
R3882	T5697	T5676	conj	unwinds,binds
R3883	T5698	T5699	det	the,fork
R3884	T5699	T5697	dobj	fork,unwinds
R3885	T5700	T5699	cc	and,fork
R3886	T5701	T5702	det	the,DNA
R3887	T5702	T5699	conj	DNA,fork
R3888	T5703	T5702	amod	nascent,DNA
R3889	T5704	T5702	amod	lagging,DNA
R3890	T5705	T5702	compound	strand,DNA
R3891	T5706	T5702	prep	on,DNA
R3892	T5707	T5708	compound	A,fork
R3893	T5708	T5706	pobj	fork,on
R3894	T5709	T5708	punct	-,fork
R3895	T5710	T5708	punct	[,fork
R3896	T5711	T5708	nummod	5,fork
R3897	T5712	T5708	punct	′,fork
R3898	T5713	T5676	punct	],binds
R3899	T5714	T5676	punct	", ",binds
R3900	T5715	T5676	cc	or,binds
R3901	T5716	T5676	conj	unwinds,binds
R3902	T5717	T5718	det	the,strand
R3903	T5718	T5716	dobj	strand,unwinds
R3904	T5719	T5718	amod	nascent,strand
R3905	T5720	T5718	amod	lagging,strand
R3906	T5721	T5716	prep	on,unwinds
R3907	T5722	T5723	compound	A,fork
R3908	T5723	T5721	pobj	fork,on
R3909	T5724	T5723	punct	-,fork
R3910	T5725	T5723	punct	[,fork
R3911	T5726	T5723	appos	3,fork
R3912	T5727	T5726	punct	′,3
R3913	T5728	T5726	punct	",",3
R3914	T5729	T5726	appos	5,3
R3915	T5730	T5729	punct	′,5
R3916	T5731	T5676	punct	],binds
R3917	T5732	T5676	punct	", ",binds
R3918	T5733	T5734	mark	as,reported
R3919	T5734	T5676	advcl	reported,binds
R3920	T5735	T5734	advmod	before,reported
R3921	T5736	T5737	punct	(,25
R3922	T5737	T5734	parataxis	25,reported
R3923	T5738	T5737	punct	),25
R3924	T5739	T5676	punct	.,binds
R3925	T5741	T5742	det	These,results
R3926	T5742	T5743	nsubj	results,indicate
R3927	T5744	T5745	mark	that,binds
R3928	T5745	T5743	advcl	binds,indicate
R3929	T5746	T5747	det	the,complex
R3930	T5747	T5745	nsubj	complex,binds
R3931	T5748	T5747	nmod	Mcm4,complex
R3932	T5749	T5748	punct	/,Mcm4
R3933	T5750	T5748	nummod	6,Mcm4
R3934	T5751	T5748	punct	/,Mcm4
R3935	T5752	T5748	nummod	7,Mcm4
R3936	T5753	T5745	prep	to,binds
R3937	T5754	T5755	amod	single,stranded
R3938	T5755	T5757	amod	stranded,DNA
R3939	T5756	T5755	punct	-,stranded
R3940	T5757	T5753	pobj	DNA,to
R3941	T5758	T5745	cc	and,binds
R3942	T5759	T5760	mark	that,activated
R3943	T5760	T5745	conj	activated,binds
R3944	T5761	T5762	poss	its,helicase
R3945	T5762	T5760	nsubjpass	helicase,activated
R3946	T5763	T5760	auxpass	is,activated
R3947	T5764	T5765	advmod	only,in
R3948	T5765	T5760	prep	in,activated
R3949	T5766	T5767	det	the,presence
R3950	T5767	T5765	pobj	presence,in
R3951	T5768	T5767	prep	of,presence
R3952	T5769	T5770	det	a,tail
R3953	T5770	T5768	pobj	tail,of
R3954	T5771	T5772	amod	single,stranded
R3955	T5772	T5770	amod	stranded,tail
R3956	T5773	T5772	punct	-,stranded
R3957	T5774	T5770	nummod	3,tail
R3958	T5775	T5774	punct	′,3
R3959	T5776	T5770	punct	-,tail
R3960	T5777	T5743	punct	.,indicate
R3967	T5933	T5934	nummod	3,extension
R3968	T5934	T5937	nsubjpass	extension,displaced
R3969	T5935	T5933	punct	′,3
R3970	T5936	T5934	punct	-,extension
R3971	T5938	T5934	prep	with,extension
R3972	T5939	T5940	nmod	poly,dT
R3973	T5940	T5942	nmod	dT,tail
R3974	T5941	T5940	punct	(,dT
R3975	T5942	T5938	pobj	tail,with
R3976	T5943	T5942	punct	),tail
R3977	T5944	T5937	auxpass	is,displaced
R3978	T5945	T5937	agent	by,displaced
R3979	T5946	T5945	pobj	Mcm4,by
R3980	T5947	T5946	punct	/,Mcm4
R3981	T5948	T5946	nummod	6,Mcm4
R3982	T5949	T5946	punct	/,Mcm4
R3983	T5950	T5946	nummod	7,Mcm4
R3984	T5952	T5953	nsubjpass	It,reported
R3985	T5954	T5953	aux	has,reported
R3986	T5955	T5953	auxpass	been,reported
R3987	T5956	T5957	mark	that,unwound
R3988	T5957	T5953	ccomp	unwound,reported
R3989	T5958	T5959	det	the,extension
R3990	T5959	T5957	nsubj	extension,unwound
R3991	T5960	T5959	nummod	3,extension
R3992	T5961	T5960	punct	′,3
R3993	T5962	T5959	punct	-,extension
R3994	T5963	T5957	aux	can,unwound
R3995	T5964	T5957	aux	be,unwound
R3996	T5965	T5957	agent	by,unwound
R3997	T5966	T5967	det	the,homohexamer
R3998	T5967	T5965	pobj	homohexamer,by
R3999	T5968	T5967	amod	archaeal,homohexamer
R4000	T5969	T5967	compound	Mcm,homohexamer
R4001	T5970	T5957	cc	but,unwound
R4002	T5971	T5957	conj	not,unwound
R4003	T5972	T5971	agent	by,not
R4004	T5973	T5974	nmod	fission,complexes
R4005	T5974	T5972	pobj	complexes,by
R4006	T5975	T5973	cc	and,fission
R4007	T5976	T5973	conj	budding,fission
R4008	T5977	T5974	compound	yeast,complexes
R4009	T5978	T5974	compound	Mcm,complexes
R4010	T5979	T5980	punct	(,27
R4011	T5980	T5953	parataxis	27,reported
R4012	T5981	T5980	nummod	14,27
R4013	T5982	T5980	punct	",",27
R4014	T5983	T5980	nummod	26,27
R4015	T5984	T5980	punct	",",27
R4016	T5985	T5980	punct	),27
R4017	T5986	T5953	punct	.,reported
R4018	T5988	T5989	nsubj	We,constructed
R4019	T5990	T5989	aux	have,constructed
R4020	T5991	T5992	nummod	3,extension
R4021	T5992	T5995	compound	extension,substrates
R4022	T5993	T5991	punct	′,3
R4023	T5994	T5992	punct	-,extension
R4024	T5995	T5989	dobj	substrates,constructed
R4025	T5996	T5995	acl	containing,substrates
R4026	T5997	T5998	nmod	poly,dT
R4027	T5998	T6000	nmod	dT,tails
R4028	T5999	T5998	punct	(,dT
R4029	T6000	T5996	dobj	tails,containing
R4030	T6001	T5998	punct	),dT
R4031	T6002	T5998	punct	", ",dT
R4032	T6003	T6004	nmod	poly,dA
R4033	T6004	T5998	conj	dA,dT
R4034	T6005	T6004	punct	(,dA
R4035	T6006	T6004	punct	),dA
R4036	T6007	T6004	punct	", ",dA
R4037	T6008	T6009	nmod	poly,dC
R4038	T6009	T6004	conj	dC,dA
R4039	T6010	T6009	punct	(,dC
R4040	T6011	T6009	punct	),dC
R4041	T6012	T6009	cc	and,dC
R4042	T6013	T6014	nmod	poly,dG
R4043	T6014	T6009	conj	dG,dC
R4044	T6015	T6014	punct	(,dG
R4045	T6016	T6014	punct	),dG
R4046	T6017	T6000	nummod	3,tails
R4047	T6018	T6017	punct	′,3
R4048	T6019	T6000	punct	-,tails
R4049	T6020	T5989	prep	in,constructed
R4050	T6021	T6020	pobj	order,in
R4051	T6022	T6023	aux	to,examine
R4052	T6023	T6021	acl	examine,order
R4053	T6024	T6025	mark	whether,displace
R4054	T6025	T6023	ccomp	displace,examine
R4055	T6026	T6027	compound	mouse,Mcm4
R4056	T6027	T6025	nsubj	Mcm4,displace
R4057	T6028	T6027	punct	/,Mcm4
R4058	T6029	T6027	nummod	6,Mcm4
R4059	T6030	T6027	punct	/,Mcm4
R4060	T6031	T6027	nummod	7,Mcm4
R4061	T6032	T6025	aux	can,displace
R4062	T6033	T6034	det	these,substrates
R4063	T6034	T6025	dobj	substrates,displace
R4064	T6035	T5989	punct	.,constructed
R4065	T6037	T6038	nsubj	We,found
R4066	T6039	T6038	advmod	first,found
R4067	T6040	T6041	mark	that,binds
R4068	T6041	T6038	ccomp	binds,found
R4069	T6042	T6041	nsubj	Mcm4,binds
R4070	T6043	T6042	punct	/,Mcm4
R4071	T6044	T6042	nummod	6,Mcm4
R4072	T6045	T6042	punct	/,Mcm4
R4073	T6046	T6042	nummod	7,Mcm4
R4074	T6047	T6041	prep	to,binds
R4075	T6048	T6049	nmod	dT,extension
R4076	T6049	T6047	pobj	extension,to
R4077	T6050	T6048	punct	-,dT
R4078	T6051	T6048	punct	", ",dT
R4079	T6052	T6048	conj	dC,dT
R4080	T6053	T6052	punct	-,dC
R4081	T6054	T6052	cc	and,dC
R4082	T6055	T6052	conj	dG,dC
R4083	T6056	T6049	punct	-,extension
R4084	T6057	T6049	nummod	3,extension
R4085	T6058	T6057	punct	′,3
R4086	T6059	T6049	punct	-,extension
R4087	T6060	T6041	prep	with,binds
R4088	T6061	T6062	amod	similar,affinity
R4089	T6062	T6060	pobj	affinity,with
R4090	T6063	T6041	punct	", ",binds
R4091	T6064	T6041	cc	and,binds
R4092	T6065	T6041	conj	to,binds
R4093	T6066	T6067	nmod	dA,extension
R4094	T6067	T6065	pobj	extension,to
R4095	T6068	T6067	punct	-,extension
R4096	T6069	T6067	nummod	3,extension
R4097	T6070	T6069	punct	′,3
R4098	T6071	T6067	punct	-,extension
R4099	T6072	T6065	prep	with,to
R4100	T6073	T6074	advmod	much,lower
R4101	T6074	T6075	amod	lower,affinity
R4102	T6075	T6072	pobj	affinity,with
R4103	T6076	T6077	punct	(,5A
R4104	T6077	T6065	parataxis	5A,to
R4105	T6078	T6077	compound	Figure,5A
R4106	T6079	T6077	punct	),5A
R4107	T6080	T6038	punct	.,found
R4108	T6082	T6083	nsubj	This,is
R4109	T6084	T6083	acomp	consistent,is
R4110	T6085	T6084	prep	with,consistent
R4111	T6086	T6087	det	the,results
R4112	T6087	T6085	pobj	results,with
R4113	T6088	T6087	amod	previous,results
R4114	T6089	T6090	mark	that,binds
R4115	T6090	T6087	acl	binds,results
R4116	T6091	T6090	nsubj	Mcm4,binds
R4117	T6092	T6091	punct	/,Mcm4
R4118	T6093	T6091	nummod	6,Mcm4
R4119	T6094	T6091	punct	/,Mcm4
R4120	T6095	T6091	nummod	7,Mcm4
R4121	T6096	T6090	prep	to,binds
R4122	T6097	T6098	compound	50mer,dT
R4123	T6098	T6096	pobj	dT,to
R4124	T6099	T6098	compound	oligo,dT
R4125	T6100	T6098	punct	-,dT
R4126	T6101	T6098	punct	", ",dT
R4127	T6102	T6103	punct	-,dC
R4128	T6103	T6098	conj	dC,dT
R4129	T6104	T6103	cc	and,dC
R4130	T6105	T6106	punct	-,dG
R4131	T6106	T6103	conj	dG,dC
R4132	T6107	T6096	punct	", ",to
R4133	T6108	T6096	cc	but,to
R4134	T6109	T6108	neg	not,but
R4135	T6110	T6096	conj	to,to
R4136	T6111	T6112	det	a,dA
R4137	T6112	T6110	pobj	dA,to
R4138	T6113	T6112	compound	50mer,dA
R4139	T6114	T6112	compound	oligo,dA
R4140	T6115	T6112	punct	-,dA
R4141	T6116	T6117	punct	(,13
R4142	T6117	T6083	parataxis	13,is
R4143	T6118	T6117	punct	),13
R4144	T6119	T6083	punct	.,is
R4145	T6121	T6122	nsubj	We,found
R4146	T6123	T6124	mark	that,displaced
R4147	T6124	T6122	ccomp	displaced,found
R4148	T6125	T6126	npadvmod	dT,tailed
R4149	T6126	T6128	amod	tailed,extension
R4150	T6127	T6126	punct	-,tailed
R4151	T6128	T6124	nsubjpass	extension,displaced
R4152	T6129	T6128	nummod	3,extension
R4153	T6130	T6129	punct	′,3
R4154	T6131	T6128	punct	-,extension
R4155	T6132	T6124	auxpass	was,displaced
R4156	T6133	T6124	advmod	efficiently,displaced
R4157	T6134	T6124	agent	by,displaced
R4158	T6135	T6134	pobj	Mcm,by
R4159	T6136	T6135	nummod	4,Mcm
R4160	T6137	T6135	punct	/,Mcm
R4161	T6138	T6135	nummod	6,Mcm
R4162	T6139	T6135	punct	/,Mcm
R4163	T6140	T6135	nummod	7,Mcm
R4164	T6141	T6142	mark	while,detected
R4165	T6142	T6124	advcl	detected,displaced
R4166	T6143	T6144	advmod	almost,no
R4167	T6144	T6142	nsubjpass	no,detected
R4168	T6145	T6144	cc	or,no
R4169	T6146	T6147	advmod	very,little
R4170	T6147	T6148	nmod	little,activity
R4171	T6148	T6144	conj	activity,no
R4172	T6149	T6142	auxpass	was,detected
R4173	T6150	T6142	prep	on,detected
R4174	T6151	T6152	amod	other,substrates
R4175	T6152	T6150	pobj	substrates,on
R4176	T6153	T6154	punct	(,5B
R4177	T6154	T6142	parataxis	5B,detected
R4178	T6155	T6154	compound	Figure,5B
R4179	T6156	T6154	cc	and,5B
R4180	T6157	T6154	conj	C,5B
R4181	T6158	T6154	punct	),5B
R4182	T6159	T6122	punct	.,found
R4183	T6161	T6162	nsubj	This,is
R4184	T6163	T6162	prep	in,is
R4185	T6164	T6165	amod	sharp,contrast
R4186	T6165	T6163	pobj	contrast,in
R4187	T6166	T6165	prep	to,contrast
R4188	T6167	T6168	nmod	yeast,helicases
R4189	T6168	T6166	pobj	helicases,to
R4190	T6169	T6168	nmod	Mcm4,helicases
R4191	T6170	T6169	punct	/,Mcm4
R4192	T6171	T6169	nummod	6,Mcm4
R4193	T6172	T6169	punct	/,Mcm4
R4194	T6173	T6169	nummod	7,Mcm4
R4195	T6174	T6175	dep	which,are
R4196	T6175	T6168	relcl	are,helicases
R4197	T6176	T6175	neg	not,are
R4198	T6177	T6175	acomp	capable,are
R4199	T6178	T6177	prep	of,capable
R4200	T6179	T6178	pcomp	unwinding,of
R4201	T6180	T6181	nummod	3,extension
R4202	T6181	T6184	compound	extension,substrates
R4203	T6182	T6180	punct	′,3
R4204	T6183	T6181	punct	-,extension
R4205	T6184	T6179	dobj	substrates,unwinding
R4206	T6185	T6162	punct	.,is
R4207	T6187	T6188	advmod	Furthermore,displaced
R4208	T6189	T6188	punct	", ",displaced
R4209	T6190	T6191	npadvmod	dT,tailed
R4210	T6191	T6193	amod	tailed,extension
R4211	T6192	T6191	punct	-,tailed
R4212	T6193	T6188	nsubjpass	extension,displaced
R4213	T6194	T6193	nummod	5,extension
R4214	T6195	T6194	punct	′,5
R4215	T6196	T6193	punct	-,extension
R4216	T6197	T6193	punct	", ",extension
R4217	T6198	T6199	prep	to,bind
R4218	T6199	T6193	relcl	bind,extension
R4219	T6200	T6198	pobj	which,to
R4220	T6201	T6199	nsubj	Mcm4,bind
R4221	T6202	T6201	punct	/,Mcm4
R4222	T6203	T6201	nummod	6,Mcm4
R4223	T6204	T6201	punct	/,Mcm4
R4224	T6205	T6201	nummod	7,Mcm4
R4225	T6206	T6199	aux	could,bind
R4226	T6207	T6188	punct	", ",displaced
R4227	T6208	T6188	auxpass	was,displaced
R4228	T6209	T6188	neg	not,displaced
R4229	T6210	T6188	agent	by,displaced
R4230	T6211	T6212	det	the,helicase
R4231	T6212	T6210	pobj	helicase,by
R4232	T6213	T6212	compound	Mcm,helicase
R4233	T6214	T6188	punct	.,displaced
R4234	T6216	T6217	det	The,addition
R4235	T6217	T6218	nsubj	addition,stimulated
R4236	T6219	T6217	prep	of,addition
R4237	T6220	T6221	det	the,tail
R4238	T6221	T6219	pobj	tail,of
R4239	T6222	T6221	nummod	5,tail
R4240	T6223	T6222	punct	′,5
R4241	T6224	T6221	punct	-,tail
R4242	T6225	T6217	prep	to,addition
R4243	T6226	T6227	npadvmod	dT,tailed
R4244	T6227	T6229	amod	tailed,extension
R4245	T6228	T6227	punct	-,tailed
R4246	T6229	T6225	pobj	extension,to
R4247	T6230	T6229	nummod	3,extension
R4248	T6231	T6230	punct	′,3
R4249	T6232	T6229	punct	-,extension
R4250	T6233	T6218	advmod	further,stimulated
R4251	T6234	T6235	det	the,displacement
R4252	T6235	T6218	dobj	displacement,stimulated
R4253	T6236	T6237	punct	(,5D
R4254	T6237	T6235	parataxis	5D,displacement
R4255	T6238	T6237	compound	Figure,5D
R4256	T6239	T6237	punct	),5D
R4257	T6240	T6218	punct	", ",stimulated
R4258	T6241	T6218	advcl	confirming,stimulated
R4259	T6242	T6243	det	the,report
R4260	T6243	T6241	dobj	report,confirming
R4261	T6244	T6243	amod	previous,report
R4262	T6245	T6246	mark	that,facilitate
R4263	T6246	T6243	acl	facilitate,report
R4264	T6247	T6248	det	the,structures
R4265	T6248	T6246	nsubj	structures,facilitate
R4266	T6249	T6248	compound	fork,structures
R4267	T6250	T6251	det	the,action
R4268	T6251	T6246	dobj	action,facilitate
R4269	T6252	T6251	compound	helicase,action
R4270	T6253	T6218	punct	.,stimulated
R4271	T6255	T6256	advmod	Thus,strengthen
R4272	T6257	T6256	punct	", ",strengthen
R4273	T6258	T6259	det	these,results
R4274	T6259	T6256	nsubj	results,strengthen
R4275	T6260	T6256	advmod	further,strengthen
R4276	T6261	T6262	poss	our,conclusion
R4277	T6262	T6256	dobj	conclusion,strengthen
R4278	T6263	T6264	mark	that,activated
R4279	T6264	T6262	acl	activated,conclusion
R4280	T6265	T6266	det	the,helicase
R4281	T6266	T6264	nsubjpass	helicase,activated
R4282	T6267	T6266	amod	mammalian,helicase
R4283	T6268	T6266	compound	Mcm,helicase
R4284	T6269	T6264	auxpass	is,activated
R4285	T6270	T6264	advmod	generally,activated
R4286	T6271	T6264	agent	by,activated
R4287	T6272	T6273	det	the,stretch
R4288	T6273	T6271	pobj	stretch,by
R4289	T6274	T6273	compound	thymine,stretch
R4290	T6275	T6273	punct	-,stretch
R4291	T6276	T6273	prep	on,stretch
R4292	T6277	T6278	det	the,tail
R4293	T6278	T6276	pobj	tail,on
R4294	T6279	T6278	nummod	3,tail
R4295	T6280	T6279	punct	′,3
R4296	T6281	T6278	punct	-,tail
R4297	T6282	T6283	amod	single,stranded
R4298	T6283	T6278	amod	stranded,tail
R4299	T6284	T6283	punct	-,stranded
R4300	T6285	T6256	punct	.,strengthen
R4301	T6404	T6405	det	The,effects
R4302	T6406	T6405	prep	of,effects
R4303	T6407	T6408	det	the,content
R4304	T6408	T6406	pobj	content,of
R4305	T6409	T6408	compound	thymine,content
R4306	T6410	T6408	prep	of,content
R4307	T6411	T6412	det	the,tail
R4308	T6412	T6410	pobj	tail,of
R4309	T6413	T6412	nummod	3,tail
R4310	T6414	T6413	punct	′,3
R4311	T6415	T6412	punct	-,tail
R4312	T6416	T6405	prep	on,effects
R4313	T6417	T6418	compound	Mcm,helicase
R4314	T6418	T6419	compound	helicase,activity
R4315	T6419	T6416	pobj	activity,on
R4316	T6421	T6422	nsubj	We,examined
R4317	T6423	T6422	advmod	next,examined
R4318	T6424	T6425	advmod	more,systematically
R4319	T6425	T6422	advmod	systematically,examined
R4320	T6426	T6427	det	the,effect
R4321	T6427	T6422	dobj	effect,examined
R4322	T6428	T6427	prep	of,effect
R4323	T6429	T6430	det	the,compositions
R4324	T6430	T6428	pobj	compositions,of
R4325	T6431	T6430	prep	of,compositions
R4326	T6432	T6433	det	the,residues
R4327	T6433	T6431	pobj	residues,of
R4328	T6434	T6433	compound	thymine,residues
R4329	T6435	T6433	prep	of,residues
R4330	T6436	T6437	det	the,tail
R4331	T6437	T6435	pobj	tail,of
R4332	T6438	T6437	nummod	3,tail
R4333	T6439	T6438	punct	′,3
R4334	T6440	T6437	punct	-,tail
R4335	T6441	T6427	prep	on,effect
R4336	T6442	T6443	det	the,activity
R4337	T6443	T6441	pobj	activity,on
R4338	T6444	T6445	compound	Mcm,helicase
R4339	T6445	T6443	compound	helicase,activity
R4340	T6446	T6422	punct	.,examined
R4341	T6448	T6449	det	A,DNA
R4342	T6449	T6456	nsubjpass	DNA,constructed
R4343	T6450	T6449	amod	partial,DNA
R4344	T6451	T6449	nmod	heteroduplex,DNA
R4345	T6452	T6449	nmod	M13,DNA
R4346	T6453	T6454	amod	single,stranded
R4347	T6454	T6449	amod	stranded,DNA
R4348	T6455	T6454	punct	-,stranded
R4349	T6457	T6449	acl	containing,DNA
R4350	T6458	T6459	det	a,region
R4351	T6459	T6457	dobj	region,containing
R4352	T6460	T6461	nummod	37,bp
R4353	T6461	T6459	compound	bp,region
R4354	T6462	T6459	compound	duplex,region
R4355	T6463	T6457	prep	in,containing
R4356	T6464	T6465	det	the,presence
R4357	T6465	T6463	pobj	presence,in
R4358	T6466	T6465	cc	or,presence
R4359	T6467	T6465	conj	absence,presence
R4360	T6468	T6465	prep	of,presence
R4361	T6469	T6470	det	the,tail
R4362	T6470	T6468	pobj	tail,of
R4363	T6471	T6472	nummod	50,nt
R4364	T6472	T6473	npadvmod	nt,long
R4365	T6473	T6470	amod	long,tail
R4366	T6474	T6470	nummod	3,tail
R4367	T6475	T6474	punct	′,3
R4368	T6476	T6470	punct	-,tail
R4369	T6477	T6470	prep	of,tail
R4370	T6478	T6479	amod	various,compositions
R4371	T6479	T6477	pobj	compositions,of
R4372	T6480	T6479	compound	nucleotide,compositions
R4373	T6481	T6456	auxpass	were,constructed
R4374	T6482	T6456	cc	and,constructed
R4375	T6483	T6484	det	the,unwinding
R4376	T6484	T6485	nsubjpass	unwinding,examined
R4377	T6485	T6456	conj	examined,constructed
R4378	T6486	T6484	prep	by,unwinding
R4379	T6487	T6488	compound	mouse,Mcm4
R4380	T6488	T6486	pobj	Mcm4,by
R4381	T6489	T6488	punct	/,Mcm4
R4382	T6490	T6488	nummod	6,Mcm4
R4383	T6491	T6488	punct	/,Mcm4
R4384	T6492	T6488	nummod	7,Mcm4
R4385	T6493	T6485	auxpass	was,examined
R4386	T6494	T6495	punct	(,6A
R4387	T6495	T6485	parataxis	6A,examined
R4388	T6496	T6495	compound	Figure,6A
R4389	T6497	T6495	punct	),6A
R4390	T6498	T6485	punct	.,examined
R4391	T6500	T6501	prep	Due,displaced
R4392	T6502	T6500	pcomp	to,Due
R4393	T6503	T6504	poss	its,processivity
R4394	T6504	T6500	pobj	processivity,Due
R4395	T6505	T6504	amod	low,processivity
R4396	T6506	T6501	punct	", ",displaced
R4397	T6507	T6501	nsubj	Mcm4,displaced
R4398	T6508	T6507	punct	/,Mcm4
R4399	T6509	T6507	nummod	6,Mcm4
R4400	T6510	T6507	punct	/,Mcm4
R4401	T6511	T6507	nummod	7,Mcm4
R4402	T6512	T6501	advmod	barely,displaced
R4403	T6513	T6514	det	the,oligonucleotide
R4404	T6514	T6501	dobj	oligonucleotide,displaced
R4405	T6515	T6514	amod	annealed,oligonucleotide
R4406	T6516	T6514	compound	37mer,oligonucleotide
R4407	T6517	T6514	prep	without,oligonucleotide
R4408	T6518	T6519	det	a,tail
R4409	T6519	T6517	pobj	tail,without
R4410	T6520	T6519	nummod	3,tail
R4411	T6521	T6520	punct	′,3
R4412	T6522	T6519	punct	-,tail
R4413	T6523	T6524	punct	(,6A
R4414	T6524	T6514	parataxis	6A,oligonucleotide
R4415	T6525	T6524	dep	37mer,6A
R4416	T6526	T6524	punct	;,6A
R4417	T6527	T6524	compound	Figure,6A
R4418	T6528	T6524	punct	),6A
R4419	T6529	T6501	punct	.,displaced
R4420	T6531	T6532	advmod	Only,activity
R4421	T6532	T6535	nsubjpass	activity,observed
R4422	T6533	T6532	amod	weak,activity
R4423	T6534	T6532	compound	helicase,activity
R4424	T6536	T6535	auxpass	was,observed
R4425	T6537	T6535	prep	on,observed
R4426	T6538	T6539	det	the,substrate
R4427	T6539	T6537	pobj	substrate,on
R4428	T6540	T6539	acl	carrying,substrate
R4429	T6541	T6542	det	the,tail
R4430	T6542	T6540	dobj	tail,carrying
R4431	T6543	T6542	nummod	3,tail
R4432	T6544	T6543	punct	′,3
R4433	T6545	T6542	punct	-,tail
R4434	T6546	T6542	prep	of,tail
R4435	T6547	T6548	nummod	25,repeats
R4436	T6548	T6546	pobj	repeats,of
R4437	T6549	T6548	prep	of,repeats
R4438	T6550	T6551	compound	TA,dinucleotide
R4439	T6551	T6549	pobj	dinucleotide,of
R4440	T6552	T6553	punct	(,6A
R4441	T6553	T6540	parataxis	6A,carrying
R4442	T6554	T6555	nummod	37,TA25
R4443	T6555	T6553	dep	TA25,6A
R4444	T6556	T6555	punct	-,TA25
R4445	T6557	T6553	punct	;,6A
R4446	T6558	T6553	compound	Figure,6A
R4447	T6559	T6553	punct	),6A
R4448	T6560	T6535	punct	", ",observed
R4449	T6561	T6562	mark	whereas,observed
R4450	T6562	T6535	advcl	observed,observed
R4451	T6563	T6564	amod	strong,activity
R4452	T6564	T6562	nsubjpass	activity,observed
R4453	T6565	T6564	compound	helicase,activity
R4454	T6566	T6562	auxpass	was,observed
R4455	T6567	T6562	prep	on,observed
R4456	T6568	T6569	nummod	37,TTA17
R4457	T6569	T6567	pobj	TTA17,on
R4458	T6570	T6569	punct	-,TTA17
R4459	T6571	T6535	punct	.,observed
R4460	T6573	T6574	nsubj	Increase,increase
R4461	T6575	T6573	prep	of,Increase
R4462	T6576	T6577	compound	thymine,content
R4463	T6577	T6575	pobj	content,of
R4464	T6578	T6573	prep	on,Increase
R4465	T6579	T6580	det	the,tail
R4466	T6580	T6578	pobj	tail,on
R4467	T6581	T6580	nummod	3,tail
R4468	T6582	T6581	punct	′,3
R4469	T6583	T6580	punct	-,tail
R4470	T6584	T6574	aux	did,increase
R4471	T6585	T6574	neg	not,increase
R4472	T6586	T6574	advmod	further,increase
R4473	T6587	T6588	det	the,extent
R4474	T6588	T6574	dobj	extent,increase
R4475	T6589	T6588	prep	of,extent
R4476	T6590	T6589	pobj	unwinding,of
R4477	T6591	T6592	punct	(,6A
R4478	T6592	T6590	parataxis	6A,unwinding
R4479	T6593	T6594	nummod	37,TTTA13
R4480	T6594	T6592	dep	TTTA13,6A
R4481	T6595	T6594	punct	-,TTTA13
R4482	T6596	T6594	punct	", ",TTTA13
R4483	T6597	T6598	nummod	37,TTTTA10
R4484	T6598	T6594	conj	TTTTA10,TTTA13
R4485	T6599	T6598	punct	-,TTTTA10
R4486	T6600	T6598	cc	and,TTTTA10
R4487	T6601	T6602	nummod	37,T50
R4488	T6602	T6598	conj	T50,TTTTA10
R4489	T6603	T6602	punct	-,T50
R4490	T6604	T6592	punct	;,6A
R4491	T6605	T6592	compound	Figure,6A
R4492	T6606	T6592	punct	),6A
R4493	T6607	T6574	punct	", ",increase
R4494	T6608	T6574	advcl	suggesting,increase
R4495	T6609	T6610	mark	that,be
R4496	T6610	T6608	ccomp	be,suggesting
R4497	T6611	T6612	nummod	17,repeats
R4498	T6612	T6610	nsubj	repeats,be
R4499	T6613	T6612	prep	of,repeats
R4500	T6614	T6615	compound	TT,dinucleotides
R4501	T6615	T6613	pobj	dinucleotides,of
R4502	T6616	T6617	punct	(,thymine
R4503	T6617	T6615	parataxis	thymine,dinucleotides
R4504	T6618	T6619	nummod	67,%
R4505	T6619	T6617	compound	%,thymine
R4506	T6620	T6617	punct	),thymine
R4507	T6621	T6610	aux	may,be
R4508	T6622	T6610	acomp	sufficient,be
R4509	T6623	T6622	prep	for,sufficient
R4510	T6624	T6623	pobj	activation,for
R4511	T6625	T6624	prep	of,activation
R4512	T6626	T6627	compound	Mcm,helicase
R4513	T6627	T6625	pobj	helicase,of
R4514	T6628	T6574	punct	.,increase
R4515	T6630	T6631	nsubjpass	It,noted
R4516	T6632	T6631	aux	should,noted
R4517	T6633	T6631	auxpass	be,noted
R4518	T6634	T6635	mark	that,anneal
R4519	T6635	T6631	ccomp	anneal,noted
R4520	T6636	T6637	det	the,tail
R4521	T6637	T6635	nsubj	tail,anneal
R4522	T6638	T6637	compound	TA25,tail
R4523	T6639	T6635	aux	would,anneal
R4524	T6640	T6635	dep	self,anneal
R4525	T6641	T6635	punct	-,anneal
R4526	T6642	T6635	cc	and,anneal
R4527	T6643	T6644	nsubj	this,prevent
R4528	T6644	T6635	conj	prevent,anneal
R4529	T6645	T6644	aux	would,prevent
R4530	T6646	T6647	det	the,loading
R4531	T6647	T6644	dobj	loading,prevent
R4532	T6648	T6647	prep	of,loading
R4533	T6649	T6648	pobj	Mcm,of
R4534	T6650	T6631	punct	", ",noted
R4535	T6651	T6652	mark	since,enhance
R4536	T6652	T6631	advcl	enhance,noted
R4537	T6653	T6654	det	the,tail
R4538	T6654	T6652	nsubj	tail,enhance
R4539	T6655	T6654	nummod	3,tail
R4540	T6656	T6655	punct	′,3
R4541	T6657	T6654	punct	-,tail
R4542	T6658	T6654	acl	containing,tail
R4543	T6659	T6658	dobj	TTAA13,containing
R4544	T6660	T6659	cc	or,TTAA13
R4545	T6661	T6659	conj	TTTAAA8,TTAA13
R4546	T6662	T6652	aux	did,enhance
R4547	T6663	T6652	neg	not,enhance
R4548	T6664	T6665	det	the,unwinding
R4549	T6665	T6652	dobj	unwinding,enhance
R4550	T6666	T6652	advmod	either,enhance
R4551	T6667	T6668	punct	(,shown
R4552	T6668	T6652	parataxis	shown,enhance
R4553	T6669	T6668	nsubj	data,shown
R4554	T6670	T6668	neg	not,shown
R4555	T6671	T6668	punct	),shown
R4556	T6672	T6631	punct	.,noted
R4557	T6674	T6675	advmod	Therefore,examined
R4558	T6676	T6675	punct	", ",examined
R4559	T6677	T6675	nsubj	we,examined
R4560	T6678	T6675	advmod	next,examined
R4561	T6679	T6680	det	the,effects
R4562	T6680	T6675	dobj	effects,examined
R4563	T6681	T6680	prep	of,effects
R4564	T6682	T6683	amod	repeating,sequences
R4565	T6683	T6681	pobj	sequences,of
R4566	T6684	T6683	prep	of,sequences
R4567	T6685	T6684	pobj	thymine,of
R4568	T6686	T6685	cc	and,thymine
R4569	T6687	T6685	conj	cytosine,thymine
R4570	T6688	T6689	punct	(,6B
R4571	T6689	T6675	parataxis	6B,examined
R4572	T6690	T6689	compound	Figure,6B
R4573	T6691	T6689	punct	),6B
R4574	T6692	T6675	punct	.,examined
R4575	T6694	T6695	amod	Partial,substrates
R4576	T6695	T6697	nsubjpass	substrates,displaced
R4577	T6696	T6695	compound	heteroduplex,substrates
R4578	T6698	T6695	acl	containing,substrates
R4579	T6699	T6700	det	the,tail
R4580	T6700	T6698	dobj	tail,containing
R4581	T6701	T6700	nummod	3,tail
R4582	T6702	T6701	punct	′,3
R4583	T6703	T6700	punct	-,tail
R4584	T6704	T6700	prep	of,tail
R4585	T6705	T6706	nmod	TC25,TTCC17
R4586	T6706	T6704	pobj	TTCC17,of
R4587	T6707	T6706	punct	", ",TTCC17
R4588	T6708	T6706	nmod	TTCC13,TTCC17
R4589	T6709	T6706	punct	", ",TTCC17
R4590	T6710	T6706	nmod	TTTCCC8,TTCC17
R4591	T6711	T6706	punct	", ",TTCC17
R4592	T6712	T6706	nmod	TTTTCCCC6,TTCC17
R4593	T6713	T6706	punct	", ",TTCC17
R4594	T6714	T6697	auxpass	were,displaced
R4595	T6715	T6697	agent	by,displaced
R4596	T6716	T6717	det	the,complex
R4597	T6717	T6715	pobj	complex,by
R4598	T6718	T6717	nmod	Mcm4,complex
R4599	T6719	T6718	punct	/,Mcm4
R4600	T6720	T6718	nummod	6,Mcm4
R4601	T6721	T6718	punct	/,Mcm4
R4602	T6722	T6718	nummod	7,Mcm4
R4603	T6723	T6697	prep	to,displaced
R4604	T6724	T6725	det	the,extent
R4605	T6725	T6723	pobj	extent,to
R4606	T6726	T6725	amod	similar,extent
R4607	T6727	T6726	prep	to,similar
R4608	T6728	T6727	pobj	that,to
R4609	T6729	T6728	acl	achieved,that
R4610	T6730	T6729	agent	by,achieved
R4611	T6731	T6730	pobj	TTA17,by
R4612	T6732	T6697	punct	", ",displaced
R4613	T6733	T6697	advcl	suggesting,displaced
R4614	T6734	T6735	mark	that,is
R4615	T6735	T6733	ccomp	is,suggesting
R4616	T6736	T6737	nummod	50,%
R4617	T6737	T6738	compound	%,content
R4618	T6738	T6735	nsubj	content,is
R4619	T6739	T6738	compound	thymine,content
R4620	T6740	T6735	acomp	sufficient,is
R4621	T6741	T6740	prep	for,sufficient
R4622	T6742	T6743	amod	full,activation
R4623	T6743	T6741	pobj	activation,for
R4624	T6744	T6743	prep	of,activation
R4625	T6745	T6746	compound	Mcm,helicase
R4626	T6746	T6744	pobj	helicase,of
R4627	T6747	T6697	punct	.,displaced
R4628	T6749	T6750	nsubjpass	TTCC13,displaced
R4629	T6751	T6749	cc	and,TTCC13
R4630	T6752	T6749	conj	TTCC17,TTCC13
R4631	T6753	T6750	auxpass	were,displaced
R4632	T6754	T6750	prep	to,displaced
R4633	T6755	T6756	det	a,extent
R4634	T6756	T6754	pobj	extent,to
R4635	T6757	T6756	amod	similar,extent
R4636	T6758	T6750	punct	", ",displaced
R4637	T6759	T6750	advcl	consistent,displaced
R4638	T6760	T6759	prep	with,consistent
R4639	T6761	T6762	det	the,result
R4640	T6762	T6760	pobj	result,with
R4641	T6763	T6762	amod	earlier,result
R4642	T6764	T6765	mark	that,is
R4643	T6765	T6762	acl	is,result
R4644	T6766	T6767	det	a,tail
R4645	T6767	T6765	nsubj	tail,is
R4646	T6768	T6769	nummod	40,nt
R4647	T6769	T6767	compound	nt,tail
R4648	T6770	T6765	acomp	sufficient,is
R4649	T6771	T6770	prep	for,sufficient
R4650	T6772	T6771	pobj	activation,for
R4651	T6773	T6774	punct	(,13
R4652	T6774	T6765	parataxis	13,is
R4653	T6775	T6774	punct	),13
R4654	T6776	T6750	punct	.,displaced
R4655	T6778	T6779	advmod	However,decreased
R4656	T6780	T6779	punct	", ",decreased
R4657	T6781	T6782	det	the,efficiency
R4658	T6782	T6779	nsubj	efficiency,decreased
R4659	T6783	T6782	prep	of,efficiency
R4660	T6784	T6785	det	the,displacement
R4661	T6785	T6783	pobj	displacement,of
R4662	T6786	T6779	advmod	significantly,decreased
R4663	T6787	T6788	mark	as,dropped
R4664	T6788	T6779	advcl	dropped,decreased
R4665	T6789	T6790	det	the,content
R4666	T6790	T6788	nsubj	content,dropped
R4667	T6791	T6790	compound	thymine,content
R4668	T6792	T6790	prep	of,content
R4669	T6793	T6794	det	the,tail
R4670	T6794	T6792	pobj	tail,of
R4671	T6795	T6794	nummod	3,tail
R4672	T6796	T6795	punct	′,3
R4673	T6797	T6794	punct	-,tail
R4674	T6798	T6788	prep	to,dropped
R4675	T6799	T6800	nummod	33,%
R4676	T6800	T6798	pobj	%,to
R4677	T6801	T6788	punct	", ",dropped
R4678	T6802	T6788	prep	as,dropped
R4679	T6803	T6802	prep	in,as
R4680	T6804	T6803	pobj	TAA17,in
R4681	T6805	T6804	cc	or,TAA17
R4682	T6806	T6804	conj	TCC17,TAA17
R4683	T6807	T6808	punct	(,6B
R4684	T6808	T6788	parataxis	6B,dropped
R4685	T6809	T6808	compound	Figure,6B
R4686	T6810	T6808	cc	and,6B
R4687	T6811	T6808	conj	C,6B
R4688	T6812	T6808	punct	),6B
R4689	T6813	T6779	punct	.,decreased
R4690	T6815	T6816	advmod	Thus,be
R4691	T6817	T6816	punct	", ",be
R4692	T6818	T6819	det	the,content
R4693	T6819	T6816	nsubj	content,be
R4694	T6820	T6819	amod	minimal,content
R4695	T6821	T6819	compound	thymine,content
R4696	T6822	T6819	acl	required,content
R4697	T6823	T6822	prep	for,required
R4698	T6824	T6823	pobj	activation,for
R4699	T6825	T6824	prep	of,activation
R4700	T6826	T6827	det	the,helicase
R4701	T6827	T6825	pobj	helicase,of
R4702	T6828	T6827	compound	Mcm,helicase
R4703	T6829	T6816	aux	may,be
R4704	T6830	T6816	advmod	somewhere,be
R4705	T6831	T6830	prep	between,somewhere
R4706	T6832	T6833	nummod	33,%
R4707	T6833	T6831	pobj	%,between
R4708	T6834	T6832	cc	and,33
R4709	T6835	T6832	conj	50,33
R4710	T6836	T6816	punct	.,be
R4711	T7008	T7009	compound	DUE,sequences
R4712	T7009	T7010	nsubj	sequences,activate
R4713	T7011	T7009	prep	from,sequences
R4714	T7012	T7013	det	the,origin
R4715	T7013	T7011	pobj	origin,from
R4716	T7014	T7015	compound	c,myc
R4717	T7015	T7013	compound	myc,origin
R4718	T7016	T7015	punct	-,myc
R4719	T7017	T7018	compound	Mcm,helicase
R4720	T7018	T7010	dobj	helicase,activate
R4721	T7020	T7021	poss	Our,studies
R4722	T7021	T7023	nsubj	studies,shown
R4723	T7022	T7021	amod	previous,studies
R4724	T7024	T7023	aux	have,shown
R4725	T7025	T7026	mark	that,serve
R4726	T7026	T7023	ccomp	serve,shown
R4727	T7027	T7028	det	the,sequences
R4728	T7028	T7026	nsubj	sequences,serve
R4729	T7029	T7028	acl	containing,sequences
R4730	T7030	T7031	amod	periodic,tracts
R4731	T7031	T7029	dobj	tracts,containing
R4732	T7032	T7033	punct	(,n
R4733	T7033	T7031	compound	n,tracts
R4734	T7034	T7033	nmod	dT,n
R4735	T7035	T7033	punct	),n
R4736	T7036	T7031	acl	derived,tracts
R4737	T7037	T7036	prep	from,derived
R4738	T7038	T7039	det	the,origin
R4739	T7039	T7037	pobj	origin,from
R4740	T7040	T7039	amod	human,origin
R4741	T7041	T7042	compound	lamin,B2
R4742	T7042	T7039	compound	B2,origin
R4743	T7043	T7044	punct	(,content
R4744	T7044	T7039	parataxis	content,origin
R4745	T7045	T7046	nummod	48,%
R4746	T7046	T7044	compound	%,content
R4747	T7047	T7044	compound	thymine,content
R4748	T7048	T7044	punct	),content
R4749	T7049	T7026	aux	can,serve
R4750	T7050	T7026	prep	as,serve
R4751	T7051	T7052	det	an,activator
R4752	T7052	T7050	pobj	activator,as
R4753	T7053	T7052	amod	efficient,activator
R4754	T7054	T7052	prep	for,activator
R4755	T7055	T7056	det	the,helicase
R4756	T7056	T7054	pobj	helicase,for
R4757	T7057	T7056	compound	Mcm,helicase
R4758	T7058	T7026	prep	on,serve
R4759	T7059	T7060	det	a,bubble
R4760	T7060	T7058	pobj	bubble,on
R4761	T7061	T7023	punct	", ",shown
R4762	T7062	T7023	cc	and,shown
R4763	T7063	T7064	nsubj	replacement,abolished
R4764	T7064	T7023	conj	abolished,shown
R4765	T7065	T7063	prep	of,replacement
R4766	T7066	T7065	pobj	thymines,of
R4767	T7067	T7063	prep	with,replacement
R4768	T7068	T7067	pobj	guanines,with
R4769	T7069	T7070	det	the,activation
R4770	T7070	T7064	dobj	activation,abolished
R4771	T7071	T7070	compound	helicase,activation
R4772	T7072	T7073	punct	(,28
R4773	T7073	T7064	parataxis	28,abolished
R4774	T7074	T7073	nummod	13,28
R4775	T7075	T7073	punct	",",28
R4776	T7076	T7073	punct	),28
R4777	T7077	T7064	punct	.,abolished
R4778	T7079	T7080	prep	In,constructed
R4779	T7081	T7079	pobj	order,In
R4780	T7082	T7083	aux	to,examine
R4781	T7083	T7081	acl	examine,order
R4782	T7084	T7085	amod	other,origins
R4783	T7085	T7083	dobj	origins,examine
R4784	T7086	T7085	amod	natural,origins
R4785	T7087	T7085	compound	replication,origins
R4786	T7088	T7085	prep	with,origins
R4787	T7089	T7090	amod	different,contents
R4788	T7090	T7088	pobj	contents,with
R4789	T7091	T7090	compound	thymine,contents
R4790	T7092	T7083	prep	for,examine
R4791	T7093	T7094	det	the,ability
R4792	T7094	T7092	pobj	ability,for
R4793	T7095	T7096	aux	to,activate
R4794	T7096	T7094	acl	activate,ability
R4795	T7097	T7098	compound	Mcm,helicase
R4796	T7098	T7096	dobj	helicase,activate
R4797	T7099	T7096	prep	on,activate
R4798	T7100	T7101	det	a,substrate
R4799	T7101	T7099	pobj	substrate,on
R4800	T7102	T7101	compound	bubble,substrate
R4801	T7103	T7080	punct	", ",constructed
R4802	T7104	T7080	nsubj	we,constructed
R4803	T7105	T7106	amod	new,substrates
R4804	T7106	T7080	dobj	substrates,constructed
R4805	T7107	T7106	compound	bubble,substrates
R4806	T7108	T7106	punct	", ",substrates
R4807	T7109	T7110	compound	Bub82,myc
R4808	T7110	T7106	appos	myc,substrates
R4809	T7111	T7110	punct	/,myc
R4810	T7112	T7110	compound	c,myc
R4811	T7113	T7110	punct	-,myc
R4812	T7114	T7106	punct	", ",substrates
R4813	T7115	T7106	acl	containing,substrates
R4814	T7116	T7115	dobj	sequences,containing
R4815	T7117	T7116	acl	derived,sequences
R4816	T7118	T7117	prep	from,derived
R4817	T7119	T7120	det	the,region
R4818	T7120	T7118	pobj	region,from
R4819	T7121	T7120	nmod	DUE,region
R4820	T7122	T7121	punct	(,DUE
R4821	T7123	T7124	compound	DNA,element
R4822	T7124	T7121	appos	element,DUE
R4823	T7125	T7124	compound	unwinding,element
R4824	T7126	T7120	punct	),region
R4825	T7127	T7120	prep	of,region
R4826	T7128	T7129	det	the,origin
R4827	T7129	T7127	pobj	origin,of
R4828	T7130	T7129	amod	human,origin
R4829	T7131	T7132	compound	c,myc
R4830	T7132	T7129	compound	myc,origin
R4831	T7133	T7132	punct	-,myc
R4832	T7134	T7135	dep	which,is
R4833	T7135	T7120	relcl	is,region
R4834	T7136	T7135	acomp	essential,is
R4835	T7137	T7136	prep	for,essential
R4836	T7138	T7139	compound	c,myc
R4837	T7139	T7141	compound	myc,activity
R4838	T7140	T7139	punct	-,myc
R4839	T7141	T7137	pobj	activity,for
R4840	T7142	T7141	compound	replicator,activity
R4841	T7143	T7144	punct	(,30
R4842	T7144	T7135	parataxis	30,is
R4843	T7145	T7144	nummod	29,30
R4844	T7146	T7144	punct	",",30
R4845	T7147	T7144	punct	),30
R4846	T7148	T7080	punct	.,constructed
R4847	T7150	T7151	det	The,segment
R4848	T7151	T7153	nsubj	segment,contains
R4849	T7152	T7151	amod	unpaired,segment
R4850	T7154	T7151	prep	in,segment
R4851	T7155	T7156	compound	Bub82,DUE
R4852	T7156	T7154	pobj	DUE,in
R4853	T7157	T7156	punct	/,DUE
R4854	T7158	T7159	compound	c,myc
R4855	T7159	T7156	compound	myc,DUE
R4856	T7160	T7159	punct	-,myc
R4857	T7161	T7156	punct	/,DUE
R4858	T7162	T7163	nummod	one,strand
R4859	T7163	T7153	dobj	strand,contains
R4860	T7164	T7163	prep	of,strand
R4861	T7165	T7164	pobj	DUE,of
R4862	T7166	T7153	cc	and,contains
R4863	T7167	T7168	nsubj	that,contains
R4864	T7168	T7153	conj	contains,contains
R4865	T7169	T7167	prep	in,that
R4866	T7170	T7171	compound	Bub82,C
R4867	T7171	T7169	pobj	C,in
R4868	T7172	T7171	punct	/,C
R4869	T7173	T7174	compound	c,myc
R4870	T7174	T7171	compound	myc,C
R4871	T7175	T7174	punct	-,myc
R4872	T7176	T7171	punct	/,C
R4873	T7177	T7171	compound	DUE,C
R4874	T7178	T7171	punct	-,C
R4875	T7179	T7180	det	another,strand
R4876	T7180	T7168	dobj	strand,contains
R4877	T7181	T7168	punct	.,contains
R4878	T7183	T7184	det	This,DUE
R4879	T7184	T7185	nsubjpass	DUE,believed
R4880	T7186	T7185	auxpass	is,believed
R4881	T7187	T7188	aux	to,coincide
R4882	T7188	T7185	xcomp	coincide,believed
R4883	T7189	T7188	prep	with,coincide
R4884	T7190	T7191	det	the,region
R4885	T7191	T7189	pobj	region,with
R4886	T7192	T7193	advmod	initially,unwound
R4887	T7193	T7191	amod	unwound,region
R4888	T7194	T7191	prep	of,region
R4889	T7195	T7196	det	the,origin
R4890	T7196	T7194	pobj	origin,of
R4891	T7197	T7198	compound	c,myc
R4892	T7198	T7196	compound	myc,origin
R4893	T7199	T7198	punct	-,myc
R4894	T7200	T7201	punct	(,29
R4895	T7201	T7188	parataxis	29,coincide
R4896	T7202	T7201	punct	),29
R4897	T7203	T7185	punct	", ",believed
R4898	T7204	T7185	cc	and,believed
R4899	T7205	T7206	poss	its,deletion
R4900	T7206	T7207	nsubj	deletion,reduced
R4901	T7207	T7185	conj	reduced,believed
R4902	T7208	T7207	advmod	substantially,reduced
R4903	T7209	T7210	det	the,activity
R4904	T7210	T7207	dobj	activity,reduced
R4905	T7211	T7210	compound	replicator,activity
R4906	T7212	T7213	punct	(,30
R4907	T7213	T7207	parataxis	30,reduced
R4908	T7214	T7213	punct	),30
R4909	T7215	T7207	punct	.,reduced
R4910	T7217	T7218	nsubj	Mcm4,unwound
R4911	T7219	T7217	punct	/,Mcm4
R4912	T7220	T7217	nummod	6,Mcm4
R4913	T7221	T7217	punct	/,Mcm4
R4914	T7222	T7217	nummod	7,Mcm4
R4915	T7223	T7224	compound	Bub82,B2
R4916	T7224	T7218	dobj	B2,unwound
R4917	T7225	T7224	punct	/,B2
R4918	T7226	T7224	compound	lamin,B2
R4919	T7227	T7228	punct	(,%
R4920	T7228	T7224	parataxis	%,B2
R4921	T7229	T7228	nmod	thymine,%
R4922	T7230	T7228	nummod	48,%
R4923	T7231	T7228	punct	),%
R4924	T7232	T7224	punct	", ",B2
R4925	T7233	T7234	compound	Bub82,DUE
R4926	T7234	T7224	conj	DUE,B2
R4927	T7235	T7234	punct	/,DUE
R4928	T7236	T7237	compound	c,myc
R4929	T7237	T7234	compound	myc,DUE
R4930	T7238	T7237	punct	-,myc
R4931	T7239	T7234	punct	/,DUE
R4932	T7240	T7241	punct	(,%
R4933	T7241	T7234	parataxis	%,DUE
R4934	T7242	T7241	nummod	37,%
R4935	T7243	T7241	punct	),%
R4936	T7244	T7234	cc	and,DUE
R4937	T7245	T7246	compound	Bub82,C
R4938	T7246	T7234	conj	C,DUE
R4939	T7247	T7246	punct	/,C
R4940	T7248	T7249	compound	c,myc
R4941	T7249	T7246	compound	myc,C
R4942	T7250	T7249	punct	-,myc
R4943	T7251	T7246	punct	/,C
R4944	T7252	T7246	compound	DUE,C
R4945	T7253	T7246	punct	-,C
R4946	T7254	T7255	punct	(,%
R4947	T7255	T7246	parataxis	%,C
R4948	T7256	T7255	nummod	39,%
R4949	T7257	T7255	punct	),%
R4950	T7258	T7218	prep	with,unwound
R4951	T7259	T7260	amod	similar,efficiency
R4952	T7260	T7258	pobj	efficiency,with
R4953	T7261	T7262	punct	(,7A
R4954	T7262	T7260	parataxis	7A,efficiency
R4955	T7263	T7262	compound	Figure,7A
R4956	T7264	T7262	punct	),7A
R4957	T7265	T7218	punct	", ",unwound
R4958	T7266	T7218	advcl	indicating,unwound
R4959	T7267	T7268	mark	that,serve
R4960	T7268	T7266	ccomp	serve,indicating
R4961	T7269	T7270	det	the,sequences
R4962	T7270	T7268	nsubj	sequences,serve
R4963	T7271	T7270	prep	from,sequences
R4964	T7272	T7273	det	the,origin
R4965	T7273	T7271	pobj	origin,from
R4966	T7274	T7275	compound	c,myc
R4967	T7275	T7273	compound	myc,origin
R4968	T7276	T7275	punct	-,myc
R4969	T7277	T7268	advmod	also,serve
R4970	T7278	T7268	aux	can,serve
R4971	T7279	T7268	prep	as,serve
R4972	T7280	T7281	amod	efficient,activators
R4973	T7281	T7279	pobj	activators,as
R4974	T7282	T7281	prep	of,activators
R4975	T7283	T7284	det	the,helicase
R4976	T7284	T7282	pobj	helicase,of
R4977	T7285	T7284	compound	Mcm,helicase
R4978	T7286	T7218	punct	.,unwound
R4979	T7288	T7289	det	This,result
R4980	T7289	T7290	nsubj	result,indicates
R4981	T7291	T7290	advmod	also,indicates
R4982	T7292	T7293	mark	that,activate
R4983	T7293	T7290	ccomp	activate,indicates
R4984	T7294	T7293	nsubj	sequences,activate
R4985	T7295	T7294	prep	with,sequences
R4986	T7296	T7297	nummod	37,%
R4987	T7297	T7298	compound	%,content
R4988	T7298	T7295	pobj	content,with
R4989	T7299	T7298	compound	thymine,content
R4990	T7300	T7293	aux	can,activate
R4991	T7301	T7293	dobj	Mcm4,activate
R4992	T7302	T7301	punct	/,Mcm4
R4993	T7303	T7301	nummod	6,Mcm4
R4994	T7304	T7301	punct	/,Mcm4
R4995	T7305	T7301	nummod	7,Mcm4
R4996	T7306	T7307	aux	to,displace
R4997	T7307	T7293	advcl	displace,activate
R4998	T7308	T7309	nummod	24,nt
R4999	T7309	T7310	compound	nt,duplex
R5000	T7310	T7307	dobj	duplex,displace
R5001	T7311	T7307	prep	on,displace
R5002	T7312	T7313	det	both,sides
R5003	T7313	T7311	pobj	sides,on
R5004	T7314	T7307	prep	in,displace
R5005	T7315	T7316	det	a,substrate
R5006	T7316	T7314	pobj	substrate,in
R5007	T7317	T7316	compound	bubble,substrate
R5008	T7318	T7293	punct	", ",activate
R5009	T7319	T7293	advcl	suggesting,activate
R5010	T7320	T7321	mark	that,affect
R5011	T7321	T7319	ccomp	affect,suggesting
R5012	T7322	T7323	det	the,content
R5013	T7323	T7321	nsubj	content,affect
R5014	T7324	T7323	preconj	not,content
R5015	T7325	T7324	advmod	only,not
R5016	T7326	T7323	compound	thymine,content
R5017	T7327	T7323	cc	but,content
R5018	T7328	T7327	advmod	also,but
R5019	T7329	T7330	det	the,context
R5020	T7330	T7323	conj	context,content
R5021	T7331	T7330	compound	sequence,context
R5022	T7332	T7321	aux	may,affect
R5023	T7333	T7334	det	the,activation
R5024	T7334	T7321	dobj	activation,affect
R5025	T7335	T7336	compound	Mcm,helicase
R5026	T7336	T7334	compound	helicase,activation
R5027	T7337	T7290	punct	.,indicates
R5028	T7339	T7340	prep	In,bound
R5029	T7341	T7342	compound	gel,shift
R5030	T7342	T7343	compound	shift,assays
R5031	T7343	T7339	pobj	assays,In
R5032	T7344	T7340	punct	", ",bound
R5033	T7345	T7340	nsubj	MCM4,bound
R5034	T7346	T7345	punct	/,MCM4
R5035	T7347	T7345	nummod	6,MCM4
R5036	T7348	T7345	punct	/,MCM4
R5037	T7349	T7345	nummod	7,MCM4
R5038	T7350	T7340	prep	to,bound
R5039	T7351	T7352	predet	all,bubbles
R5040	T7352	T7350	pobj	bubbles,to
R5041	T7353	T7352	det	the,bubbles
R5042	T7354	T7352	nummod	three,bubbles
R5043	T7355	T7340	punct	", ",bound
R5044	T7356	T7357	mark	although,is
R5045	T7357	T7340	advcl	is,bound
R5046	T7358	T7359	det	the,affinity
R5047	T7359	T7357	nsubj	affinity,is
R5048	T7360	T7359	prep	to,affinity
R5049	T7361	T7362	compound	Bub82,DUE
R5050	T7362	T7360	pobj	DUE,to
R5051	T7363	T7362	punct	/,DUE
R5052	T7364	T7365	compound	c,myc
R5053	T7365	T7362	compound	myc,DUE
R5054	T7366	T7365	punct	-,myc
R5055	T7367	T7362	punct	/,DUE
R5056	T7368	T7360	cc	and,to
R5057	T7369	T7360	conj	to,to
R5058	T7370	T7371	compound	Bub82,C
R5059	T7371	T7369	pobj	C,to
R5060	T7372	T7371	punct	/,C
R5061	T7373	T7374	compound	c,myc
R5062	T7374	T7371	compound	myc,C
R5063	T7375	T7374	punct	-,myc
R5064	T7376	T7371	punct	/,C
R5065	T7377	T7371	compound	DUE,C
R5066	T7378	T7371	punct	-,C
R5067	T7379	T7380	advmod	slightly,lower
R5068	T7380	T7357	acomp	lower,is
R5069	T7381	T7380	prep	than,lower
R5070	T7382	T7381	pobj	that,than
R5071	T7383	T7382	prep	to,that
R5072	T7384	T7385	nmod	Bub,B2
R5073	T7385	T7383	pobj	B2,to
R5074	T7386	T7385	punct	-,B2
R5075	T7387	T7385	nummod	82,B2
R5076	T7388	T7385	punct	/,B2
R5077	T7389	T7385	compound	lamin,B2
R5078	T7390	T7391	punct	(,7B
R5079	T7391	T7357	parataxis	7B,is
R5080	T7392	T7391	compound	Figure,7B
R5081	T7393	T7391	punct	),7B
R5082	T7394	T7340	punct	.,bound
R5083	T7396	T7397	advmod	Therefore,correlate
R5084	T7398	T7397	punct	", ",correlate
R5085	T7399	T7400	nmod	helicase,activities
R5086	T7400	T7397	nsubj	activities,correlate
R5087	T7401	T7399	cc	and,helicase
R5088	T7402	T7403	compound	DNA,binding
R5089	T7403	T7399	conj	binding,helicase
R5090	T7404	T7397	aux	do,correlate
R5091	T7405	T7397	neg	not,correlate
R5092	T7406	T7397	advmod	necessarily,correlate
R5093	T7407	T7397	prep	with,correlate
R5094	T7408	T7409	det	each,other
R5095	T7409	T7407	pobj	other,with
R5096	T7410	T7397	punct	.,correlate
R5097	T7412	T7413	nsubjpass	This,indicated
R5098	T7414	T7413	auxpass	was,indicated
R5099	T7415	T7413	advmod	also,indicated
R5100	T7416	T7413	agent	by,indicated
R5101	T7417	T7418	poss	our,results
R5102	T7418	T7416	pobj	results,by
R5103	T7419	T7418	amod	previous,results
R5104	T7420	T7421	mark	that,bind
R5105	T7421	T7418	acl	bind,results
R5106	T7422	T7423	nmod	guanine,stretches
R5107	T7423	T7421	nsubj	stretches,bind
R5108	T7424	T7422	cc	and,guanine
R5109	T7425	T7422	conj	cytosine,guanine
R5110	T7426	T7421	aux	can,bind
R5111	T7427	T7421	prep	to,bind
R5112	T7428	T7427	pobj	Mcm,to
R5113	T7429	T7421	cc	but,bind
R5114	T7430	T7431	aux	can,activate
R5115	T7431	T7421	conj	activate,bind
R5116	T7432	T7431	neg	not,activate
R5117	T7433	T7434	det	the,helicase
R5118	T7434	T7431	dobj	helicase,activate
R5119	T7435	T7436	punct	(,13
R5120	T7436	T7431	parataxis	13,activate
R5121	T7437	T7436	punct	),13
R5122	T7438	T7413	punct	.,indicated
R5124	T7722	T7723	compound	DNA,unwinding
R5125	T7723	T7724	nsubjpass	unwinding,inhibited
R5126	T7725	T7723	prep	by,unwinding
R5127	T7726	T7725	pobj	Mcm4,by
R5128	T7727	T7726	punct	/,Mcm4
R5129	T7728	T7726	nummod	6,Mcm4
R5130	T7729	T7726	punct	/,Mcm4
R5131	T7730	T7726	nummod	7,Mcm4
R5132	T7731	T7724	auxpass	is,inhibited
R5133	T7732	T7724	agent	by,inhibited
R5134	T7733	T7734	det	the,sequences
R5135	T7734	T7732	pobj	sequences,by
R5136	T7735	T7736	npadvmod	GC,rich
R5137	T7736	T7734	amod	rich,sequences
R5138	T7737	T7736	punct	-,rich
R5139	T7738	T7734	prep	on,sequences
R5140	T7739	T7740	det	the,segment
R5141	T7740	T7738	pobj	segment,on
R5142	T7741	T7740	compound	duplex,segment
R5143	T7743	T7744	poss	Our,results
R5144	T7744	T7745	nsubj	results,indicate
R5145	T7746	T7747	mark	that,required
R5146	T7747	T7745	ccomp	required,indicate
R5147	T7748	T7749	npadvmod	thymine,rich
R5148	T7749	T7751	amod	rich,DNA
R5149	T7750	T7749	punct	-,rich
R5150	T7751	T7747	nsubjpass	DNA,required
R5151	T7752	T7753	amod	single,stranded
R5152	T7753	T7751	amod	stranded,DNA
R5153	T7754	T7753	punct	-,stranded
R5154	T7755	T7747	auxpass	is,required
R5155	T7756	T7747	prep	for,required
R5156	T7757	T7758	amod	initial,loading
R5157	T7758	T7756	pobj	loading,for
R5158	T7759	T7758	cc	and,loading
R5159	T7760	T7758	conj	activation,loading
R5160	T7761	T7758	prep	of,loading
R5161	T7762	T7763	det	the,helicase
R5162	T7763	T7761	pobj	helicase,of
R5163	T7764	T7763	compound	Mcm,helicase
R5164	T7765	T7745	punct	.,indicate
R5165	T7767	T7768	advmod	However,known
R5166	T7769	T7768	punct	", ",known
R5167	T7770	T7768	nsubjpass	it,known
R5168	T7771	T7768	auxpass	is,known
R5169	T7772	T7768	neg	not,known
R5170	T7773	T7774	mark	whether,required
R5171	T7774	T7768	ccomp	required,known
R5172	T7775	T7776	compound	thymine,sequences
R5173	T7776	T7774	nsubjpass	sequences,required
R5174	T7777	T7774	auxpass	are,required
R5175	T7778	T7774	prep	for,required
R5176	T7779	T7780	amod	processive,unwinding
R5177	T7780	T7778	pobj	unwinding,for
R5178	T7781	T7780	prep	of,unwinding
R5179	T7782	T7783	compound	duplex,DNA
R5180	T7783	T7781	pobj	DNA,of
R5181	T7784	T7768	punct	.,known
R5182	T7786	T7787	advmod	Therefore,examined
R5183	T7788	T7787	punct	", ",examined
R5184	T7789	T7787	nsubj	we,examined
R5185	T7790	T7787	aux	have,examined
R5186	T7791	T7792	mark	whether,affects
R5187	T7792	T7787	ccomp	affects,examined
R5188	T7793	T7794	det	the,composition
R5189	T7794	T7792	nsubj	composition,affects
R5190	T7795	T7794	compound	nucleotide,composition
R5191	T7796	T7794	prep	of,composition
R5192	T7797	T7798	det	the,region
R5193	T7798	T7796	pobj	region,of
R5194	T7799	T7798	compound	duplex,region
R5195	T7800	T7801	poss	its,activity
R5196	T7801	T7792	dobj	activity,affects
R5197	T7802	T7801	compound	unwinding,activity
R5198	T7803	T7787	punct	.,examined
R5199	T7805	T7806	aux	To,address
R5200	T7806	T7807	advcl	address,constructed
R5201	T7808	T7809	det	this,issue
R5202	T7809	T7806	dobj	issue,address
R5203	T7810	T7807	punct	", ",constructed
R5204	T7811	T7807	nsubj	we,constructed
R5205	T7812	T7813	det	a,series
R5206	T7813	T7807	dobj	series,constructed
R5207	T7814	T7813	prep	of,series
R5208	T7815	T7816	npadvmod	T,tailed
R5209	T7816	T7818	amod	tailed,structures
R5210	T7817	T7816	punct	-,tailed
R5211	T7818	T7814	pobj	structures,of
R5212	T7819	T7820	compound	Y,fork
R5213	T7820	T7818	compound	fork,structures
R5214	T7821	T7820	punct	-,fork
R5215	T7822	T7818	punct	(,structures
R5216	T7823	T7824	compound	T,fork
R5217	T7824	T7818	appos	fork,structures
R5218	T7825	T7824	punct	-,fork
R5219	T7826	T7818	punct	),structures
R5220	T7827	T7818	acl	containing,structures
R5221	T7828	T7829	amod	various,sequences
R5222	T7829	T7827	dobj	sequences,containing
R5223	T7830	T7807	prep	in,constructed
R5224	T7831	T7832	det	the,segment
R5225	T7832	T7830	pobj	segment,in
R5226	T7833	T7832	compound	duplex,segment
R5227	T7834	T7807	punct	.,constructed
R5228	T7836	T7837	nsubj	They,carried
R5229	T7838	T7839	amod	varied,contents
R5230	T7839	T7837	dobj	contents,carried
R5231	T7840	T7839	prep	of,contents
R5232	T7841	T7842	compound	cytosine,residues
R5233	T7842	T7840	pobj	residues,of
R5234	T7843	T7837	prep	on,carried
R5235	T7844	T7845	det	the,strand
R5236	T7845	T7843	pobj	strand,on
R5237	T7846	T7847	nummod	3,tail
R5238	T7847	T7845	compound	tail,strand
R5239	T7848	T7846	punct	′,3
R5240	T7849	T7847	punct	-,tail
R5241	T7850	T7837	punct	.,carried
R5242	T7852	T7853	prep	In,bound
R5243	T7854	T7855	compound	gel,shift
R5244	T7855	T7856	compound	shift,assays
R5245	T7856	T7852	pobj	assays,In
R5246	T7857	T7853	punct	", ",bound
R5247	T7858	T7853	nsubj	Mcm4,bound
R5248	T7859	T7858	punct	/,Mcm4
R5249	T7860	T7858	nummod	6,Mcm4
R5250	T7861	T7858	punct	/,Mcm4
R5251	T7862	T7858	nummod	7,Mcm4
R5252	T7863	T7853	prep	to,bound
R5253	T7864	T7865	det	these,substrates
R5254	T7865	T7863	pobj	substrates,to
R5255	T7866	T7867	compound	Y,fork
R5256	T7867	T7865	compound	fork,substrates
R5257	T7868	T7867	punct	-,fork
R5258	T7869	T7853	prep	with,bound
R5259	T7870	T7871	amod	identical,affinity
R5260	T7871	T7869	pobj	affinity,with
R5261	T7872	T7873	punct	(,8A
R5262	T7873	T7871	parataxis	8A,affinity
R5263	T7874	T7873	compound	Figure,8A
R5264	T7875	T7873	punct	),8A
R5265	T7876	T7853	punct	", ",bound
R5266	T7877	T7853	advcl	consistent,bound
R5267	T7878	T7877	prep	with,consistent
R5268	T7879	T7880	det	the,notion
R5269	T7880	T7878	pobj	notion,with
R5270	T7881	T7882	mark	that,binds
R5271	T7882	T7880	acl	binds,notion
R5272	T7883	T7884	det	the,Mcm4
R5273	T7884	T7882	nsubj	Mcm4,binds
R5274	T7885	T7884	punct	/,Mcm4
R5275	T7886	T7884	nummod	6,Mcm4
R5276	T7887	T7884	punct	/,Mcm4
R5277	T7888	T7884	nummod	7,Mcm4
R5278	T7889	T7882	prep	to,binds
R5279	T7890	T7891	amod	single,stranded
R5280	T7891	T7893	amod	stranded,tails
R5281	T7892	T7891	punct	-,stranded
R5282	T7893	T7889	pobj	tails,to
R5283	T7894	T7853	punct	.,bound
R5284	T7896	T7897	advmod	However,displaced
R5285	T7898	T7897	punct	", ",displaced
R5286	T7899	T7897	prep	in,displaced
R5287	T7900	T7901	compound	DNA,helicase
R5288	T7901	T7902	compound	helicase,assays
R5289	T7902	T7899	pobj	assays,in
R5290	T7903	T7897	punct	", ",displaced
R5291	T7904	T7905	compound	T,fork
R5292	T7905	T7897	nsubjpass	fork,displaced
R5293	T7906	T7905	punct	-,fork
R5294	T7907	T7905	punct	/,fork
R5295	T7908	T7909	punct	(,G
R5296	T7909	T7905	appos	G,fork
R5297	T7910	T7909	nmod	C,G
R5298	T7911	T7909	punct	:,G
R5299	T7912	T7909	punct	),G
R5300	T7913	T7909	nummod	49,G
R5301	T7914	T7897	auxpass	was,displaced
R5302	T7915	T7897	advmod	hardly,displaced
R5303	T7916	T7897	agent	by,displaced
R5304	T7917	T7916	pobj	Mcm4,by
R5305	T7918	T7917	punct	/,Mcm4
R5306	T7919	T7917	nummod	6,Mcm4
R5307	T7920	T7917	punct	/,Mcm4
R5308	T7921	T7917	nummod	7,Mcm4
R5309	T7922	T7897	punct	", ",displaced
R5310	T7923	T7897	cc	but,displaced
R5311	T7924	T7925	auxpass	was,displaced
R5312	T7925	T7897	conj	displaced,displaced
R5313	T7926	T7925	advmod	readily,displaced
R5314	T7927	T7925	agent	by,displaced
R5315	T7928	T7929	compound	SV40,helicase
R5316	T7929	T7927	pobj	helicase,by
R5317	T7930	T7931	compound	T,antigen
R5318	T7931	T7929	compound	antigen,helicase
R5319	T7932	T7931	punct	-,antigen
R5320	T7933	T7929	compound	DNA,helicase
R5321	T7934	T7935	punct	(,8B
R5322	T7935	T7925	parataxis	8B,displaced
R5323	T7936	T7935	compound	Figure,8B
R5324	T7937	T7935	punct	),8B
R5325	T7938	T7897	punct	.,displaced
R5326	T7940	T7941	advmod	When,inserted
R5327	T7941	T7946	advcl	inserted,increased
R5328	T7942	T7941	nsubjpass	thymine,inserted
R5329	T7943	T7942	cc	or,thymine
R5330	T7944	T7942	conj	adenine,thymine
R5331	T7945	T7941	auxpass	is,inserted
R5332	T7947	T7941	prep	as,inserted
R5333	T7948	T7949	det	every,nucleotide
R5334	T7949	T7947	pobj	nucleotide,as
R5335	T7950	T7949	amod	third,nucleotide
R5336	T7951	T7952	punct	(,repeats
R5337	T7952	T7949	parataxis	repeats,nucleotide
R5338	T7953	T7952	prep	of,repeats
R5339	T7954	T7953	pobj	CCT,of
R5340	T7955	T7954	cc	or,CCT
R5341	T7956	T7954	conj	CCA,CCT
R5342	T7957	T7952	punct	),repeats
R5343	T7958	T7946	punct	", ",increased
R5344	T7959	T7960	det	the,extent
R5345	T7960	T7946	nsubj	extent,increased
R5346	T7961	T7960	prep	of,extent
R5347	T7962	T7961	pobj	unwinding,of
R5348	T7963	T7964	punct	(,GGA
R5349	T7964	T7946	dobj	GGA,increased
R5350	T7965	T7966	nmod	T,fork
R5351	T7966	T7964	nmod	fork,GGA
R5352	T7967	T7966	punct	-,fork
R5353	T7968	T7964	punct	/,GGA
R5354	T7969	T7964	punct	(,GGA
R5355	T7970	T7964	nmod	CCT,GGA
R5356	T7971	T7964	punct	:,GGA
R5357	T7972	T7964	punct	),GGA
R5358	T7973	T7964	nummod	16,GGA
R5359	T7974	T7964	cc	and,GGA
R5360	T7975	T7976	nmod	T,fork
R5361	T7976	T7978	nmod	fork,GGT
R5362	T7977	T7976	punct	-,fork
R5363	T7978	T7964	conj	GGT,GGA
R5364	T7979	T7978	punct	/,GGT
R5365	T7980	T7978	punct	(,GGT
R5366	T7981	T7978	nmod	CCA,GGT
R5367	T7982	T7978	punct	:,GGT
R5368	T7983	T7978	punct	),GGT
R5369	T7984	T7978	nummod	16,GGT
R5370	T7985	T7946	punct	),increased
R5371	T7986	T7946	punct	.,increased
R5372	T7988	T7989	det	The,efficiency
R5373	T7989	T7990	nsubjpass	efficiency,correlated
R5374	T7991	T7989	prep	of,efficiency
R5375	T7992	T7991	pobj	unwinding,of
R5376	T7993	T7990	auxpass	is,correlated
R5377	T7994	T7990	advmod	roughly,correlated
R5378	T7995	T7990	prep	with,correlated
R5379	T7996	T7997	det	the,content
R5380	T7997	T7995	pobj	content,with
R5381	T7998	T7997	prep	of,content
R5382	T7999	T8000	compound	GC,pairs
R5383	T8000	T7998	pobj	pairs,of
R5384	T8001	T8000	prep	in,pairs
R5385	T8002	T8003	det	the,segment
R5386	T8003	T8001	pobj	segment,in
R5387	T8004	T8003	compound	duplex,segment
R5388	T8005	T8006	punct	[,GGTT
R5389	T8006	T8000	appos	GGTT,pairs
R5390	T8007	T8008	nmod	T,fork
R5391	T8008	T8006	nmod	fork,GGTT
R5392	T8009	T8008	punct	-,fork
R5393	T8010	T8006	punct	/,GGTT
R5394	T8011	T8006	punct	(,GGTT
R5395	T8012	T8006	nmod	CCAA,GGTT
R5396	T8013	T8006	punct	:,GGTT
R5397	T8014	T8006	punct	),GGTT
R5398	T8015	T8006	nummod	12,GGTT
R5399	T8016	T8006	punct	", ",GGTT
R5400	T8017	T8018	nmod	T,fork
R5401	T8018	T8020	nmod	fork,GGAA
R5402	T8019	T8018	punct	-,fork
R5403	T8020	T8006	conj	GGAA,GGTT
R5404	T8021	T8020	punct	/,GGAA
R5405	T8022	T8020	punct	(,GGAA
R5406	T8023	T8020	nmod	CCTT,GGAA
R5407	T8024	T8020	punct	:,GGAA
R5408	T8025	T8020	punct	),GGAA
R5409	T8026	T8020	nummod	12,GGAA
R5410	T8027	T8020	punct	", ",GGAA
R5411	T8028	T8029	nmod	T,fork
R5412	T8029	T8031	nmod	fork,GTTT
R5413	T8030	T8029	punct	-,fork
R5414	T8031	T8020	conj	GTTT,GGAA
R5415	T8032	T8031	punct	/,GTTT
R5416	T8033	T8031	punct	(,GTTT
R5417	T8034	T8031	nmod	CAAA,GTTT
R5418	T8035	T8031	punct	:,GTTT
R5419	T8036	T8031	punct	),GTTT
R5420	T8037	T8031	nummod	12,GTTT
R5421	T8038	T8031	cc	and,GTTT
R5422	T8039	T8040	nmod	T,fork
R5423	T8040	T8042	nmod	fork,GAAA
R5424	T8041	T8040	punct	-,fork
R5425	T8042	T8031	conj	GAAA,GTTT
R5426	T8043	T8042	punct	/,GAAA
R5427	T8044	T8042	punct	(,GAAA
R5428	T8045	T8042	nmod	CTTT,GAAA
R5429	T8046	T8042	punct	:,GAAA
R5430	T8047	T8042	punct	),GAAA
R5431	T8048	T8042	nummod	12,GAAA
R5432	T8049	T8050	punct	;,8B
R5433	T8050	T7990	parataxis	8B,correlated
R5434	T8051	T8050	compound	Figure,8B
R5435	T8052	T8050	punct	],8B
R5436	T8053	T7990	punct	.,correlated
R5437	T8055	T8056	nsubj	It,appears
R5438	T8057	T8058	mark	that,unwound
R5439	T8058	T8056	ccomp	unwound,appears
R5440	T8059	T8060	det	the,segment
R5441	T8060	T8058	nsubjpass	segment,unwound
R5442	T8061	T8060	compound	duplex,segment
R5443	T8062	T8060	acl	containing,segment
R5444	T8063	T8064	punct	<,50
R5445	T8064	T8065	nummod	50,%
R5446	T8065	T8066	compound	%,pairs
R5447	T8066	T8062	dobj	pairs,containing
R5448	T8067	T8066	compound	GC,pairs
R5449	T8068	T8058	auxpass	is,unwound
R5450	T8069	T8058	advmod	efficiently,unwound
R5451	T8070	T8071	punct	(,8C
R5452	T8071	T8058	parataxis	8C,unwound
R5453	T8072	T8071	compound	Figure,8C
R5454	T8073	T8071	punct	),8C
R5455	T8074	T8056	punct	.,appears
R5456	T8076	T8077	nsubj	We,examined
R5457	T8078	T8077	advmod	next,examined
R5458	T8079	T8080	det	the,effect
R5459	T8080	T8077	dobj	effect,examined
R5460	T8081	T8080	prep	of,effect
R5461	T8082	T8083	npadvmod	GC,rich
R5462	T8083	T8085	amod	rich,segments
R5463	T8084	T8083	punct	-,rich
R5464	T8085	T8081	pobj	segments,of
R5465	T8086	T8080	prep	on,effect
R5466	T8087	T8088	det	a,DNA
R5467	T8088	T8086	pobj	DNA,on
R5468	T8089	T8088	amod	partial,DNA
R5469	T8090	T8088	compound	heteroduplex,DNA
R5470	T8091	T8088	prep	on,DNA
R5471	T8092	T8093	det	a,DNA
R5472	T8093	T8091	pobj	DNA,on
R5473	T8094	T8095	amod	single,stranded
R5474	T8095	T8093	amod	stranded,DNA
R5475	T8096	T8095	punct	-,stranded
R5476	T8097	T8093	amod	circular,DNA
R5477	T8098	T8077	punct	.,examined
R5478	T8100	T8101	nsubj	We,constructed
R5479	T8102	T8101	aux	have,constructed
R5480	T8103	T8104	nummod	two,sets
R5481	T8104	T8101	dobj	sets,constructed
R5482	T8105	T8104	prep	of,sets
R5483	T8106	T8107	npadvmod	5,tailed
R5484	T8107	T8110	amod	tailed,substrates
R5485	T8108	T8106	punct	′,5
R5486	T8109	T8107	punct	-,tailed
R5487	T8110	T8105	pobj	substrates,of
R5488	T8111	T8112	amod	partial,heteroduplex
R5489	T8112	T8110	compound	heteroduplex,substrates
R5490	T8113	T8110	compound	DNA,substrates
R5491	T8114	T8110	acl	containing,substrates
R5492	T8115	T8116	compound	duplex,regions
R5493	T8116	T8114	dobj	regions,containing
R5494	T8117	T8116	prep	of,regions
R5495	T8118	T8119	amod	variable,lengths
R5496	T8119	T8117	pobj	lengths,of
R5497	T8120	T8104	punct	;,sets
R5498	T8121	T8104	appos	one,sets
R5499	T8122	T8121	prep	on,one
R5500	T8123	T8124	compound	M13mp18,vector
R5501	T8124	T8122	pobj	vector,on
R5502	T8125	T8121	cc	and,one
R5503	T8126	T8127	det	the,other
R5504	T8127	T8121	conj	other,one
R5505	T8128	T8127	prep	on,other
R5506	T8129	T8128	pobj	M13mp19,on
R5507	T8130	T8121	acl	containing,one
R5508	T8131	T8132	det	a,segment
R5509	T8132	T8130	dobj	segment,containing
R5510	T8133	T8134	nummod	66,nt
R5511	T8134	T8135	npadvmod	nt,long
R5512	T8135	T8132	amod	long,segment
R5513	T8136	T8137	npadvmod	G,rich
R5514	T8137	T8132	amod	rich,segment
R5515	T8138	T8137	punct	-,rich
R5516	T8139	T8132	advmod	downstream,segment
R5517	T8140	T8139	prep	of,downstream
R5518	T8141	T8142	det	the,oligonucleotide
R5519	T8142	T8140	pobj	oligonucleotide,of
R5520	T8143	T8142	amod	hybridizing,oligonucleotide
R5521	T8144	T8101	punct	.,constructed
R5522	T8146	T8147	nsubj	We,found
R5523	T8148	T8149	mark	that,displace
R5524	T8149	T8147	ccomp	displace,found
R5525	T8150	T8151	det	the,helicase
R5526	T8151	T8149	nsubj	helicase,displace
R5527	T8152	T8151	nmod	Mcm4,helicase
R5528	T8153	T8152	punct	/,Mcm4
R5529	T8154	T8152	nummod	6,Mcm4
R5530	T8155	T8152	punct	/,Mcm4
R5531	T8156	T8152	nummod	7,Mcm4
R5532	T8157	T8149	aux	can,displace
R5533	T8158	T8159	compound	duplex,DNA
R5534	T8159	T8149	dobj	DNA,displace
R5535	T8160	T8161	quantmod	up,350
R5536	T8161	T8163	nummod	350,nt
R5537	T8162	T8161	quantmod	to,350
R5538	T8163	T8164	npadvmod	nt,long
R5539	T8164	T8159	amod	long,DNA
R5540	T8165	T8149	prep	on,displace
R5541	T8166	T8167	poss	its,own
R5542	T8167	T8165	pobj	own,on
R5543	T8168	T8149	prep	on,displace
R5544	T8169	T8170	det	the,substrate
R5545	T8170	T8168	pobj	substrate,on
R5546	T8171	T8170	amod	former,substrate
R5547	T8172	T8173	punct	(,8D
R5548	T8173	T8149	parataxis	8D,displace
R5549	T8174	T8173	compound	Figure,8D
R5550	T8175	T8173	punct	", ",8D
R5551	T8176	T8173	amod	left,8D
R5552	T8177	T8173	punct	),8D
R5553	T8178	T8147	punct	", ",found
R5554	T8179	T8147	advcl	consistent,found
R5555	T8180	T8179	prep	with,consistent
R5556	T8181	T8182	amod	previous,results
R5557	T8182	T8180	pobj	results,with
R5558	T8183	T8184	punct	(,13
R5559	T8184	T8182	parataxis	13,results
R5560	T8185	T8184	punct	),13
R5561	T8186	T8147	punct	.,found
R5562	T8188	T8189	prep	In,inhibited
R5563	T8190	T8188	pobj	contrast,In
R5564	T8191	T8189	punct	", ",inhibited
R5565	T8192	T8189	nsubjpass	displacement,inhibited
R5566	T8193	T8189	auxpass	was,inhibited
R5567	T8194	T8189	prep	over,inhibited
R5568	T8195	T8196	det	the,segments
R5569	T8196	T8194	pobj	segments,over
R5570	T8197	T8198	npadvmod	GC,rich
R5571	T8198	T8196	amod	rich,segments
R5572	T8199	T8198	punct	-,rich
R5573	T8200	T8196	prep	on,segments
R5574	T8201	T8202	det	the,substrate
R5575	T8202	T8200	pobj	substrate,on
R5576	T8203	T8202	amod	latter,substrate
R5577	T8204	T8205	punct	(,panel
R5578	T8205	T8196	parataxis	panel,segments
R5579	T8206	T8207	compound	Figure,8D
R5580	T8207	T8205	dep	8D,panel
R5581	T8208	T8205	punct	", ",panel
R5582	T8209	T8205	amod	right,panel
R5583	T8210	T8205	punct	),panel
R5584	T8211	T8189	punct	", ",inhibited
R5585	T8212	T8213	mark	although,observed
R5586	T8213	T8189	advcl	observed,inhibited
R5587	T8214	T8213	nsubjpass	displacement,observed
R5588	T8215	T8216	quantmod	up,250
R5589	T8216	T8218	nummod	250,nt
R5590	T8217	T8216	quantmod	to,250
R5591	T8218	T8214	appos	nt,displacement
R5592	T8219	T8218	prep	in,nt
R5593	T8220	T8219	pobj	length,in
R5594	T8221	T8218	punct	", ",nt
R5595	T8222	T8223	dep	albeit,at
R5596	T8223	T8218	prep	at,nt
R5597	T8224	T8225	det	a,level
R5598	T8225	T8223	pobj	level,at
R5599	T8226	T8225	amod	reduced,level
R5600	T8227	T8213	punct	", ",observed
R5601	T8228	T8213	auxpass	was,observed
R5602	T8229	T8213	prep	at,observed
R5603	T8230	T8231	det	a,concentration
R5604	T8231	T8229	pobj	concentration,at
R5605	T8232	T8231	amod	high,concentration
R5606	T8233	T8231	prep	of,concentration
R5607	T8234	T8235	nmod	Mcm4,complex
R5608	T8235	T8233	pobj	complex,of
R5609	T8236	T8234	punct	/,Mcm4
R5610	T8237	T8234	nummod	6,Mcm4
R5611	T8238	T8234	punct	/,Mcm4
R5612	T8239	T8234	nummod	7,Mcm4
R5613	T8240	T8189	punct	.,inhibited
R5614	T8242	T8243	det	This,result
R5615	T8243	T8244	nsubj	result,is
R5616	T8245	T8244	acomp	consistent,is
R5617	T8246	T8245	prep	with,consistent
R5618	T8247	T8246	pobj	that,with
R5619	T8248	T8247	prep	on,that
R5620	T8249	T8250	compound	Y,fork
R5621	T8250	T8248	pobj	fork,on
R5622	T8251	T8250	punct	-,fork
R5623	T8252	T8244	cc	and,is
R5624	T8253	T8244	conj	indicates,is
R5625	T8254	T8255	mark	that,is
R5626	T8255	T8253	ccomp	is,indicates
R5627	T8256	T8257	npadvmod	GC,rich
R5628	T8257	T8259	amod	rich,segment
R5629	T8258	T8257	punct	-,rich
R5630	T8259	T8255	nsubj	segment,is
R5631	T8260	T8259	compound	duplex,segment
R5632	T8261	T8255	acomp	inhibitory,is
R5633	T8262	T8261	prep	for,inhibitory
R5634	T8263	T8262	pobj	unwinding,for
R5635	T8264	T8263	prep	by,unwinding
R5636	T8265	T8266	det	the,helicase
R5637	T8266	T8264	pobj	helicase,by
R5638	T8267	T8266	compound	Mcm,helicase
R5639	T8268	T8244	punct	.,is
R5640	T8270	T8271	det	These,results
R5641	T8271	T8272	nsubj	results,indicate
R5642	T8273	T8272	aux	may,indicate
R5643	T8274	T8275	mark	that,facilitate
R5644	T8275	T8272	ccomp	facilitate,indicate
R5645	T8276	T8277	det	the,presence
R5646	T8277	T8275	nsubj	presence,facilitate
R5647	T8278	T8277	prep	of,presence
R5648	T8279	T8280	compound	thymine,adenine
R5649	T8280	T8282	compound	adenine,pairs
R5650	T8281	T8280	punct	–,adenine
R5651	T8282	T8278	pobj	pairs,of
R5652	T8283	T8282	prep	with,pairs
R5653	T8284	T8285	det	a,frequency
R5654	T8285	T8283	pobj	frequency,with
R5655	T8286	T8285	amod	certain,frequency
R5656	T8287	T8277	prep	in,presence
R5657	T8288	T8289	det	the,region
R5658	T8289	T8287	pobj	region,in
R5659	T8290	T8289	compound	duplex,region
R5660	T8291	T8275	aux	may,facilitate
R5661	T8292	T8293	amod	continuous,unwinding
R5662	T8293	T8275	dobj	unwinding,facilitate
R5663	T8294	T8293	prep	by,unwinding
R5664	T8295	T8294	pobj	Mcm4,by
R5665	T8296	T8295	punct	/,Mcm4
R5666	T8297	T8295	nummod	6,Mcm4
R5667	T8298	T8295	punct	/,Mcm4
R5668	T8299	T8295	nummod	7,Mcm4
R5669	T8300	T8272	punct	.,indicate
R5670	T8302	T8303	advmod	Alternatively,is
R5671	T8304	T8303	punct	", ",is
R5672	T8305	T8303	nsubj	Mcm4,is
R5673	T8306	T8305	punct	/,Mcm4
R5674	T8307	T8305	nummod	6,Mcm4
R5675	T8308	T8305	punct	/,Mcm4
R5676	T8309	T8305	nummod	7,Mcm4
R5677	T8310	T8303	neg	not,is
R5678	T8311	T8303	acomp	efficient,is
R5679	T8312	T8311	advmod	enough,efficient
R5680	T8313	T8314	aux	to,displace
R5681	T8314	T8311	xcomp	displace,efficient
R5682	T8315	T8316	advmod	thermodynamically,stable
R5683	T8316	T8317	amod	stable,segment
R5684	T8317	T8314	dobj	segment,displace
R5685	T8318	T8319	npadvmod	GC,paired
R5686	T8319	T8317	amod	paired,segment
R5687	T8320	T8319	punct	-,paired
R5688	T8321	T8319	npadvmod	base,paired
R5689	T8322	T8319	punct	-,paired
R5690	T8323	T8303	punct	.,is
R5691	T8325	T8326	prep	In,designed
R5692	T8327	T8325	pobj	order,In
R5693	T8328	T8329	aux	to,distinguish
R5694	T8329	T8327	acl	distinguish,order
R5695	T8330	T8331	det	these,possibilities
R5696	T8331	T8329	dobj	possibilities,distinguish
R5697	T8332	T8331	nummod	two,possibilities
R5698	T8333	T8326	punct	", ",designed
R5699	T8334	T8326	nsubj	we,designed
R5700	T8335	T8336	det	a,substrate
R5701	T8336	T8326	dobj	substrate,designed
R5702	T8337	T8336	amod	new,substrate
R5703	T8338	T8339	compound	T,fork
R5704	T8339	T8336	compound	fork,substrate
R5705	T8340	T8339	punct	-,fork
R5706	T8341	T8336	acl	containing,substrate
R5707	T8342	T8343	nmod	inosine,residue
R5708	T8343	T8341	dobj	residue,containing
R5709	T8344	T8342	punct	(,inosine
R5710	T8345	T8342	appos	I,inosine
R5711	T8346	T8343	punct	),residue
R5712	T8347	T8348	advmod	instead,of
R5713	T8348	T8343	prep	of,residue
R5714	T8349	T8350	compound	guanosine,residue
R5715	T8350	T8348	pobj	residue,of
R5716	T8351	T8326	punct	.,designed
R5717	T8353	T8354	det	The,stability
R5718	T8354	T8356	nsubj	stability,is
R5719	T8355	T8354	amod	thermal,stability
R5720	T8357	T8354	prep	of,stability
R5721	T8358	T8359	det	the,pair
R5722	T8359	T8357	pobj	pair,of
R5723	T8360	T8361	nmod	I,C
R5724	T8361	T8359	compound	C,pair
R5725	T8362	T8361	punct	:,C
R5726	T8363	T8359	compound	base,pair
R5727	T8364	T8356	acomp	lower,is
R5728	T8365	T8364	prep	than,lower
R5729	T8366	T8365	pobj	that,than
R5730	T8367	T8366	prep	of,that
R5731	T8368	T8369	det	the,pair
R5732	T8369	T8367	pobj	pair,of
R5733	T8370	T8371	nmod	G,C
R5734	T8371	T8369	compound	C,pair
R5735	T8372	T8371	punct	:,C
R5736	T8373	T8369	compound	base,pair
R5737	T8374	T8356	prep	in,is
R5738	T8375	T8376	compound	duplex,DNA
R5739	T8376	T8374	pobj	DNA,in
R5740	T8377	T8356	prep	due,is
R5741	T8378	T8377	pcomp	to,due
R5742	T8379	T8377	pobj	loss,due
R5743	T8380	T8379	prep	of,loss
R5744	T8381	T8382	nummod	one,bond
R5745	T8382	T8380	pobj	bond,of
R5746	T8383	T8382	compound	hydrogen,bond
R5747	T8384	T8356	cc	and,is
R5748	T8385	T8356	conj	is,is
R5749	T8386	T8387	advmod	even,lower
R5750	T8387	T8385	acomp	lower,is
R5751	T8388	T8387	prep	than,lower
R5752	T8389	T8390	nmod	A,T
R5753	T8390	T8392	compound	T,pair
R5754	T8391	T8390	punct	:,T
R5755	T8392	T8388	pobj	pair,than
R5756	T8393	T8392	compound	base,pair
R5757	T8394	T8395	punct	(,31
R5758	T8395	T8392	parataxis	31,pair
R5759	T8396	T8395	punct	),31
R5760	T8397	T8356	punct	.,is
R5761	T8399	T8400	nsubj	We,constructed
R5762	T8401	T8402	det	the,substrates
R5763	T8402	T8400	dobj	substrates,constructed
R5764	T8403	T8404	npadvmod	T,tailed
R5765	T8404	T8402	amod	tailed,substrates
R5766	T8405	T8404	punct	-,tailed
R5767	T8406	T8402	compound	fork,substrates
R5768	T8407	T8402	acl	containing,substrates
R5769	T8408	T8409	det	the,segment
R5770	T8409	T8407	dobj	segment,containing
R5771	T8410	T8411	punct	(,CGG
R5772	T8411	T8409	nmod	CGG,segment
R5773	T8412	T8411	nmod	GCC,CGG
R5774	T8413	T8411	punct	:,CGG
R5775	T8414	T8411	punct	),CGG
R5776	T8415	T8411	nummod	10,CGG
R5777	T8416	T8411	punct	", ",CGG
R5778	T8417	T8418	punct	(,CIG
R5779	T8418	T8411	conj	CIG,CGG
R5780	T8419	T8418	nmod	GCC,CIG
R5781	T8420	T8418	punct	:,CIG
R5782	T8421	T8418	punct	),CIG
R5783	T8422	T8418	nummod	10,CIG
R5784	T8423	T8418	cc	or,CIG
R5785	T8424	T8425	punct	(,CTT
R5786	T8425	T8418	conj	CTT,CIG
R5787	T8426	T8425	nmod	GAA,CTT
R5788	T8427	T8425	punct	:,CTT
R5789	T8428	T8425	punct	),CTT
R5790	T8429	T8425	nummod	10,CTT
R5791	T8430	T8409	compound	duplex,segment
R5792	T8431	T8409	compound	DNA,segment
R5793	T8432	T8433	punct	(,8E
R5794	T8433	T8407	parataxis	8E,containing
R5795	T8434	T8433	compound	Figure,8E
R5796	T8435	T8433	punct	),8E
R5797	T8436	T8400	punct	.,constructed
R5798	T8438	T8439	nsubj	Mcm4,displaced
R5799	T8440	T8438	punct	/,Mcm4
R5800	T8441	T8438	nummod	6,Mcm4
R5801	T8442	T8438	punct	/,Mcm4
R5802	T8443	T8438	nummod	7,Mcm4
R5803	T8444	T8439	advmod	hardly,displaced
R5804	T8445	T8446	det	the,duplex
R5805	T8446	T8439	dobj	duplex,displaced
R5806	T8447	T8448	nummod	31,nt
R5807	T8448	T8449	npadvmod	nt,long
R5808	T8449	T8446	amod	long,duplex
R5809	T8450	T8446	prep	of,duplex
R5810	T8451	T8452	nmod	GCC,CGG
R5811	T8452	T8454	compound	CGG,repeats
R5812	T8453	T8452	punct	:,CGG
R5813	T8454	T8450	pobj	repeats,of
R5814	T8455	T8439	punct	", ",displaced
R5815	T8456	T8457	mark	whereas,displaced
R5816	T8457	T8439	advcl	displaced,displaced
R5817	T8458	T8459	det	the,CTT
R5818	T8459	T8457	nsubjpass	CTT,displaced
R5819	T8460	T8461	nmod	T,fork
R5820	T8461	T8459	nmod	fork,CTT
R5821	T8462	T8461	punct	-,fork
R5822	T8463	T8459	punct	/,CTT
R5823	T8464	T8459	punct	(,CTT
R5824	T8465	T8459	nmod	GAA,CTT
R5825	T8466	T8459	punct	:,CTT
R5826	T8467	T8459	punct	),CTT
R5827	T8468	T8459	nummod	10,CTT
R5828	T8469	T8457	auxpass	was,displaced
R5829	T8470	T8457	advmod	efficiently,displaced
R5830	T8471	T8457	punct	", ",displaced
R5831	T8472	T8473	mark	as,described
R5832	T8473	T8457	advcl	described,displaced
R5833	T8474	T8473	advmod	above,described
R5834	T8475	T8439	punct	.,displaced
R5835	T8477	T8478	nmod	T,fork
R5836	T8478	T8480	nmod	fork,CIG
R5837	T8479	T8478	punct	-,fork
R5838	T8480	T8485	nsubjpass	CIG,displaced
R5839	T8481	T8480	punct	/,CIG
R5840	T8482	T8480	punct	(,CIG
R5841	T8483	T8480	nmod	GCC,CIG
R5842	T8484	T8480	punct	:,CIG
R5843	T8486	T8480	punct	),CIG
R5844	T8487	T8480	nummod	10,CIG
R5845	T8488	T8485	auxpass	was,displaced
R5846	T8489	T8485	prep	with,displaced
R5847	T8490	T8489	pobj	efficiency,with
R5848	T8491	T8492	advmod	much,greater
R5849	T8492	T8490	amod	greater,efficiency
R5850	T8493	T8492	prep	than,greater
R5851	T8494	T8493	pobj	that,than
R5852	T8495	T8494	prep	of,that
R5853	T8496	T8497	nmod	T,fork
R5854	T8497	T8499	nmod	fork,CGG
R5855	T8498	T8497	punct	-,fork
R5856	T8499	T8495	pobj	CGG,of
R5857	T8500	T8497	punct	/,fork
R5858	T8501	T8499	punct	(,CGG
R5859	T8502	T8499	nmod	GCC,CGG
R5860	T8503	T8499	punct	:,CGG
R5861	T8504	T8499	punct	),CGG
R5862	T8505	T8499	nummod	10,CGG
R5863	T8506	T8485	punct	", ",displaced
R5864	T8507	T8485	advcl	indicating,displaced
R5865	T8508	T8509	mark	that,is
R5866	T8509	T8507	ccomp	is,indicating
R5867	T8510	T8511	det	the,thermostability
R5868	T8511	T8509	nsubj	thermostability,is
R5869	T8512	T8511	prep	of,thermostability
R5870	T8513	T8514	det	the,segment
R5871	T8514	T8512	pobj	segment,of
R5872	T8515	T8514	compound	duplex,segment
R5873	T8516	T8511	punct	", ",thermostability
R5874	T8517	T8511	cc	not,thermostability
R5875	T8518	T8519	det	the,lack
R5876	T8519	T8511	conj	lack,thermostability
R5877	T8520	T8519	prep	of,lack
R5878	T8521	T8522	compound	AT,pair
R5879	T8522	T8520	pobj	pair,of
R5880	T8523	T8522	compound	base,pair
R5881	T8524	T8509	punct	", ",is
R5882	T8525	T8509	acomp	responsible,is
R5883	T8526	T8525	prep	for,responsible
R5884	T8527	T8526	pobj	inability,for
R5885	T8528	T8527	prep	of,inability
R5886	T8529	T8528	pobj	Mcm4,of
R5887	T8530	T8529	punct	/,Mcm4
R5888	T8531	T8529	nummod	6,Mcm4
R5889	T8532	T8529	punct	/,Mcm4
R5890	T8533	T8529	nummod	7,Mcm4
R5891	T8534	T8535	aux	to,displace
R5892	T8535	T8527	acl	displace,inability
R5893	T8536	T8537	det	the,duplex
R5894	T8537	T8535	dobj	duplex,displace
R5895	T8538	T8485	punct	.,displaced
R5899	T8794	T8792	attr	factor,is
R5900	T8795	T8794	amod	central,factor
R5901	T8796	T8794	prep	for,factor
R5902	T8797	T8796	pobj	operation,for
R5903	T8798	T8797	prep	of,operation
R5904	T8799	T8800	compound	replication,forks
R5905	T8800	T8798	pobj	forks,of
R5906	T8801	T8792	punct	.,is
R5907	T8803	T8804	nsubj	It,plays
R5908	T8805	T8804	advmod	also,plays
R5909	T8806	T8807	det	a,role
R5910	T8807	T8804	dobj	role,plays
R5911	T8808	T8807	amod	crucial,role
R5912	T8809	T8804	prep	in,plays
R5913	T8810	T8811	det	the,step
R5914	T8811	T8809	pobj	step,in
R5915	T8812	T8811	compound	initiation,step
R5916	T8813	T8804	prep	at,plays
R5917	T8814	T8815	det	the,origins
R5918	T8815	T8813	pobj	origins,at
R5919	T8816	T8815	compound	replication,origins
R5920	T8817	T8804	punct	.,plays
R5921	T8819	T8820	advmod	Therefore,is
R5922	T8821	T8820	punct	", ",is
R5923	T8822	T8823	det	the,elucidation
R5924	T8823	T8820	nsubj	elucidation,is
R5925	T8824	T8823	prep	of,elucidation
R5926	T8825	T8826	advmod	how,interact
R5927	T8826	T8824	pobj	interact,of
R5928	T8827	T8828	det	the,helicases
R5929	T8828	T8826	nsubj	helicases,interact
R5930	T8829	T8828	amod	replicative,helicases
R5931	T8830	T8826	prep	with,interact
R5932	T8831	T8832	compound	DNA,substrate
R5933	T8832	T8830	pobj	substrate,with
R5934	T8833	T8826	cc	and,interact
R5935	T8834	T8835	advmod	how,activated
R5936	T8835	T8826	conj	activated,interact
R5937	T8836	T8835	nsubjpass	they,activated
R5938	T8837	T8835	auxpass	are,activated
R5939	T8838	T8820	acomp	crucial,is
R5940	T8839	T8838	prep	for,crucial
R5941	T8840	T8839	pcomp	understanding,for
R5942	T8841	T8842	det	the,basis
R5943	T8842	T8840	dobj	basis,understanding
R5944	T8843	T8842	amod	molecular,basis
R5945	T8844	T8842	prep	of,basis
R5946	T8845	T8844	pobj	initiation,of
R5947	T8846	T8845	prep	of,initiation
R5948	T8847	T8848	compound	DNA,replication
R5949	T8848	T8846	pobj	replication,of
R5950	T8849	T8820	punct	.,is
R5951	T8851	T8852	nsubjpass	It,believed
R5952	T8853	T8852	auxpass	is,believed
R5953	T8854	T8852	advmod	now,believed
R5954	T8855	T8856	mark	that,is
R5955	T8856	T8852	ccomp	is,believed
R5956	T8857	T8856	nsubj	Mcm,is
R5957	T8858	T8859	det	a,component
R5958	T8859	T8856	attr	component,is
R5959	T8860	T8859	amod	major,component
R5960	T8861	T8859	prep	of,component
R5961	T8862	T8863	det	the,helicase
R5962	T8863	T8861	pobj	helicase,of
R5963	T8864	T8863	amod	eukaryotic,helicase
R5964	T8865	T8863	amod	replicative,helicase
R5965	T8866	T8852	punct	.,believed
R5966	T8868	T8869	prep	In,conducted
R5967	T8870	T8871	det	this,report
R5968	T8871	T8868	pobj	report,In
R5969	T8872	T8869	punct	", ",conducted
R5970	T8873	T8869	nsubj	we,conducted
R5971	T8874	T8869	aux	have,conducted
R5972	T8875	T8876	amod	detailed,analyses
R5973	T8876	T8869	dobj	analyses,conducted
R5974	T8877	T8876	prep	on,analyses
R5975	T8878	T8879	nmod	binding,actions
R5976	T8879	T8877	pobj	actions,on
R5977	T8880	T8878	cc	and,binding
R5978	T8881	T8878	conj	helicase,binding
R5979	T8882	T8879	prep	of,actions
R5980	T8883	T8884	compound	mouse,Mcm4
R5981	T8884	T8882	pobj	Mcm4,of
R5982	T8885	T8884	punct	/,Mcm4
R5983	T8886	T8884	nummod	6,Mcm4
R5984	T8887	T8884	punct	/,Mcm4
R5985	T8888	T8884	nummod	7,Mcm4
R5986	T8889	T8869	advcl	using,conducted
R5987	T8890	T8891	amod	various,substrates
R5988	T8891	T8889	dobj	substrates,using
R5989	T8892	T8891	prep	including,substrates
R5990	T8893	T8894	nmod	fork,structures
R5991	T8894	T8892	pobj	structures,including
R5992	T8895	T8893	cc	and,fork
R5993	T8896	T8893	conj	bubble,fork
R5994	T8897	T8869	punct	.,conducted
R5997	T9089	T9088	prep	of,Modes
R5998	T9090	T9089	pobj	binding,of
R5999	T9091	T9090	cc	and,binding
R6000	T9092	T9090	conj	activation,binding
R6001	T9093	T9090	prep	of,binding
R6002	T9094	T9095	nmod	Mcm4,helicase
R6003	T9095	T9093	pobj	helicase,of
R6004	T9096	T9094	punct	/,Mcm4
R6005	T9097	T9094	nummod	6,Mcm4
R6006	T9098	T9094	punct	/,Mcm4
R6007	T9099	T9094	nummod	7,Mcm4
R6008	T9100	T9090	prep	on,binding
R6009	T9101	T9102	det	a,structure
R6010	T9102	T9100	pobj	structure,on
R6011	T9103	T9102	compound	bubble,structure
R6012	T9105	T9106	nsubjpass	Initiation,associated
R6013	T9107	T9105	prep	of,Initiation
R6014	T9108	T9109	compound	DNA,replication
R6015	T9109	T9107	pobj	replication,of
R6016	T9110	T9106	auxpass	is,associated
R6017	T9111	T9106	prep	with,associated
R6018	T9112	T9113	amod	localized,melting
R6019	T9113	T9111	pobj	melting,with
R6020	T9114	T9113	prep	of,melting
R6021	T9115	T9116	compound	duplex,DNA
R6022	T9116	T9114	pobj	DNA,of
R6023	T9117	T9113	amod	near,melting
R6024	T9118	T9119	compound	replication,origins
R6025	T9119	T9117	npadvmod	origins,near
R6026	T9120	T9106	punct	.,associated
R6027	T9122	T9123	nsubjpass	Helicases,loaded
R6028	T9124	T9123	auxpass	are,loaded
R6029	T9125	T9123	prep	onto,loaded
R6030	T9126	T9127	compound	replication,forks
R6031	T9127	T9125	pobj	forks,onto
R6032	T9128	T9123	prep	through,loaded
R6033	T9129	T9130	det	the,region
R6034	T9130	T9128	pobj	region,through
R6035	T9131	T9130	amod	melted,region
R6036	T9132	T9130	punct	", ",region
R6037	T9133	T9130	acl	induced,region
R6038	T9134	T9133	prep	by,induced
R6039	T9135	T9136	compound	initiator,binding
R6040	T9136	T9134	pobj	binding,by
R6041	T9137	T9123	punct	", ",loaded
R6042	T9138	T9123	prep	in,loaded
R6043	T9139	T9138	pobj	bacteria,in
R6044	T9140	T9141	punct	(,32
R6045	T9141	T9123	parataxis	32,loaded
R6046	T9142	T9141	punct	),32
R6047	T9143	T9123	punct	.,loaded
R6048	T9145	T9146	nsubj	We,reported
R6049	T9147	T9146	advmod	previously,reported
R6050	T9148	T9149	mark	that,loaded
R6051	T9149	T9146	ccomp	loaded,reported
R6052	T9150	T9149	nsubjpass	Mcm4,loaded
R6053	T9151	T9150	punct	/,Mcm4
R6054	T9152	T9150	nummod	6,Mcm4
R6055	T9153	T9150	punct	/,Mcm4
R6056	T9154	T9150	nummod	7,Mcm4
R6057	T9155	T9149	aux	can,loaded
R6058	T9156	T9149	auxpass	be,loaded
R6059	T9157	T9149	prep	onto,loaded
R6060	T9158	T9159	det	a,structure
R6061	T9159	T9157	pobj	structure,onto
R6062	T9160	T9161	npadvmod	bubble,like
R6063	T9161	T9159	amod	like,structure
R6064	T9162	T9161	punct	-,like
R6065	T9163	T9146	cc	and,reported
R6066	T9164	T9165	aux	can,serve
R6067	T9165	T9146	conj	serve,reported
R6068	T9166	T9165	prep	as,serve
R6069	T9167	T9168	det	a,helicase
R6070	T9168	T9166	pobj	helicase,as
R6071	T9169	T9168	compound	DNA,helicase
R6072	T9170	T9165	prep	at,serve
R6073	T9171	T9172	det	the,forks
R6074	T9172	T9170	pobj	forks,at
R6075	T9173	T9174	punct	(,13
R6076	T9174	T9165	parataxis	13,serve
R6077	T9175	T9174	punct	),13
R6078	T9176	T9146	punct	.,reported
R6079	T9178	T9179	det	The,ability
R6080	T9179	T9180	nsubj	ability,indicates
R6081	T9181	T9179	prep	of,ability
R6082	T9182	T9181	pobj	Mcm4,of
R6083	T9183	T9182	punct	/,Mcm4
R6084	T9184	T9182	nummod	6,Mcm4
R6085	T9185	T9182	punct	/,Mcm4
R6086	T9186	T9182	nummod	7,Mcm4
R6087	T9187	T9188	aux	to,unwind
R6088	T9188	T9179	acl	unwind,ability
R6089	T9189	T9190	det	the,substrate
R6090	T9190	T9188	dobj	substrate,unwind
R6091	T9191	T9190	compound	bubble,substrate
R6092	T9192	T9190	cc	but,substrate
R6093	T9193	T9192	neg	not,but
R6094	T9194	T9195	det	the,DNA
R6095	T9195	T9190	conj	DNA,substrate
R6096	T9196	T9195	amod	conventional,DNA
R6097	T9197	T9195	compound	duplex,DNA
R6098	T9198	T9199	punct	(,2
R6099	T9199	T9188	parataxis	2,unwind
R6100	T9200	T9199	nmod	Figures,2
R6101	T9201	T9199	cc	and,2
R6102	T9202	T9199	conj	13,2
R6103	T9203	T9199	punct	),2
R6104	T9204	T9205	mark	that,loaded
R6105	T9205	T9180	ccomp	loaded,indicates
R6106	T9206	T9205	nsubjpass	Mcm,loaded
R6107	T9207	T9205	aux	can,loaded
R6108	T9208	T9205	auxpass	be,loaded
R6109	T9209	T9205	prep	through,loaded
R6110	T9210	T9211	det	the,segment
R6111	T9211	T9209	pobj	segment,through
R6112	T9212	T9213	amod	single,stranded
R6113	T9213	T9211	amod	stranded,segment
R6114	T9214	T9213	punct	-,stranded
R6115	T9215	T9211	prep	of,segment
R6116	T9216	T9217	det	the,bubble
R6117	T9217	T9215	pobj	bubble,of
R6118	T9218	T9180	punct	.,indicates
R6119	T9220	T9221	advmod	Furthermore,displays
R6120	T9222	T9221	punct	", ",displays
R6121	T9223	T9224	det	the,complex
R6122	T9224	T9221	nsubj	complex,displays
R6123	T9225	T9224	nmod	Mcm4,complex
R6124	T9226	T9225	punct	/,Mcm4
R6125	T9227	T9225	nummod	6,Mcm4
R6126	T9228	T9225	punct	/,Mcm4
R6127	T9229	T9225	nummod	7,Mcm4
R6128	T9230	T9231	amod	marked,preference
R6129	T9231	T9221	dobj	preference,displays
R6130	T9232	T9231	prep	for,preference
R6131	T9233	T9234	npadvmod	thymine,rich
R6132	T9234	T9236	amod	rich,sequences
R6133	T9235	T9234	punct	-,rich
R6134	T9236	T9232	pobj	sequences,for
R6135	T9237	T9221	prep	within,displays
R6136	T9238	T9239	det	the,segment
R6137	T9239	T9237	pobj	segment,within
R6138	T9240	T9241	amod	single,stranded
R6139	T9241	T9239	amod	stranded,segment
R6140	T9242	T9241	punct	-,stranded
R6141	T9243	T9239	prep	for,segment
R6142	T9244	T9245	compound	helicase,activation
R6143	T9245	T9243	pobj	activation,for
R6144	T9246	T9247	punct	(,13
R6145	T9247	T9221	parataxis	13,displays
R6146	T9248	T9247	punct	),13
R6147	T9249	T9221	punct	.,displays
R6148	T9251	T9252	det	The,results
R6149	T9252	T9253	nsubj	results,indicate
R6150	T9254	T9252	prep	in,results
R6151	T9255	T9256	det	this,report
R6152	T9256	T9254	pobj	report,in
R6153	T9257	T9258	mark	that,depend
R6154	T9258	T9253	ccomp	depend,indicate
R6155	T9259	T9260	compound	helicase,action
R6156	T9260	T9258	nsubj	action,depend
R6157	T9261	T9260	prep	of,action
R6158	T9262	T9261	pobj	Mcm4,of
R6159	T9263	T9262	punct	/,Mcm4
R6160	T9264	T9262	nummod	6,Mcm4
R6161	T9265	T9262	punct	/,Mcm4
R6162	T9266	T9262	nummod	7,Mcm4
R6163	T9267	T9260	prep	on,action
R6164	T9268	T9269	amod	synthetic,bubbles
R6165	T9269	T9267	pobj	bubbles,on
R6166	T9270	T9258	aux	may,depend
R6167	T9271	T9258	prep	on,depend
R6168	T9272	T9273	det	the,presence
R6169	T9273	T9271	pobj	presence,on
R6170	T9274	T9273	prep	of,presence
R6171	T9275	T9276	det	an,region
R6172	T9276	T9274	pobj	region,of
R6173	T9277	T9276	amod	unpaired,region
R6174	T9278	T9276	prep	of,region
R6175	T9279	T9280	amod	sufficient,length
R6176	T9280	T9278	pobj	length,of
R6177	T9281	T9282	punct	(,nt
R6178	T9282	T9280	parataxis	nt,length
R6179	T9283	T9284	advmod	at,least
R6180	T9284	T9285	advmod	least,40
R6181	T9285	T9282	nummod	40,nt
R6182	T9286	T9282	punct	),nt
R6183	T9287	T9276	punct	", ",region
R6184	T9288	T9289	dep	which,permit
R6185	T9289	T9276	relcl	permit,region
R6186	T9290	T9289	aux	may,permit
R6187	T9291	T9289	dobj	assembly,permit
R6188	T9292	T9291	prep	of,assembly
R6189	T9293	T9294	det	a,complex
R6190	T9294	T9292	pobj	complex,of
R6191	T9295	T9296	amod	double,hexameric
R6192	T9296	T9294	amod	hexameric,complex
R6193	T9297	T9296	punct	-,hexameric
R6194	T9298	T9294	prep	on,complex
R6195	T9299	T9300	det	the,DNA
R6196	T9300	T9298	pobj	DNA,on
R6197	T9301	T9300	compound	substrate,DNA
R6198	T9302	T9294	punct	", ",complex
R6199	T9303	T9294	amod	similar,complex
R6200	T9304	T9303	prep	to,similar
R6201	T9305	T9306	compound	SV40,protein
R6202	T9306	T9304	pobj	protein,to
R6203	T9307	T9308	compound	T,antigen
R6204	T9308	T9306	compound	antigen,protein
R6205	T9309	T9308	punct	-,antigen
R6206	T9310	T9311	punct	(,2A
R6207	T9311	T9304	parataxis	2A,to
R6208	T9312	T9311	compound	Figure,2A
R6209	T9313	T9311	punct	),2A
R6210	T9314	T9315	punct	(,21
R6211	T9315	T9289	parataxis	21,permit
R6212	T9316	T9315	punct	),21
R6213	T9317	T9253	punct	.,indicate
R6214	T9319	T9320	advmod	When,replaced
R6215	T9320	T9335	advcl	replaced,reduced
R6216	T9321	T9322	nummod	one,strand
R6217	T9322	T9320	nsubjpass	strand,replaced
R6218	T9323	T9322	prep	of,strand
R6219	T9324	T9325	det	the,segment
R6220	T9325	T9323	pobj	segment,of
R6221	T9326	T9327	amod	single,stranded
R6222	T9327	T9325	amod	stranded,segment
R6223	T9328	T9327	punct	-,stranded
R6224	T9329	T9322	prep	in,strand
R6225	T9330	T9331	npadvmod	T,rich
R6226	T9331	T9333	amod	rich,bubble
R6227	T9332	T9331	punct	-,rich
R6228	T9333	T9329	pobj	bubble,in
R6229	T9334	T9320	auxpass	was,replaced
R6230	T9336	T9320	agent	by,replaced
R6231	T9337	T9338	npadvmod	guanine,rich
R6232	T9338	T9340	amod	rich,sequences
R6233	T9339	T9338	punct	-,rich
R6234	T9340	T9336	pobj	sequences,by
R6235	T9341	T9335	punct	", ",reduced
R6236	T9342	T9343	det	the,efficiency
R6237	T9343	T9335	nsubjpass	efficiency,reduced
R6238	T9344	T9343	prep	of,efficiency
R6239	T9345	T9344	pobj	unwinding,of
R6240	T9346	T9335	auxpass	was,reduced
R6241	T9347	T9335	advmod	significantly,reduced
R6242	T9348	T9335	punct	.,reduced
R6243	T9350	T9351	poss	Our,analyses
R6244	T9351	T9353	nsubj	analyses,showed
R6245	T9352	T9351	compound	footprinting,analyses
R6246	T9354	T9355	mark	that,protects
R6247	T9355	T9353	ccomp	protects,showed
R6248	T9356	T9355	nsubj	Mcm4,protects
R6249	T9357	T9356	punct	/,Mcm4
R6250	T9358	T9356	nummod	6,Mcm4
R6251	T9359	T9356	punct	/,Mcm4
R6252	T9360	T9356	nummod	7,Mcm4
R6253	T9361	T9355	advmod	strongly,protects
R6254	T9362	T9363	nummod	25,nt
R6255	T9363	T9364	nmod	nt,segment
R6256	T9364	T9355	dobj	segment,protects
R6257	T9365	T9366	amod	single,stranded
R6258	T9366	T9364	amod	stranded,segment
R6259	T9367	T9366	punct	-,stranded
R6260	T9368	T9364	compound	DNA,segment
R6261	T9369	T9364	amod	adjacent,segment
R6262	T9370	T9369	prep	to,adjacent
R6263	T9371	T9372	det	each,point
R6264	T9372	T9370	pobj	point,to
R6265	T9373	T9372	compound	branch,point
R6266	T9374	T9369	cc	and,adjacent
R6267	T9375	T9369	conj	proximal,adjacent
R6268	T9376	T9375	prep	to,proximal
R6269	T9377	T9378	nummod	5,ends
R6270	T9378	T9376	pobj	ends,to
R6271	T9379	T9377	punct	′,5
R6272	T9380	T9378	prep	of,ends
R6273	T9381	T9382	det	both,strands
R6274	T9382	T9380	pobj	strands,of
R6275	T9383	T9382	prep	of,strands
R6276	T9384	T9385	det	the,bubble
R6277	T9385	T9383	pobj	bubble,of
R6278	T9386	T9353	punct	.,showed
R6279	T9388	T9389	prep	At,is
R6280	T9390	T9391	det	each,fork
R6281	T9391	T9388	pobj	fork,At
R6282	T9392	T9389	punct	", ",is
R6283	T9393	T9394	nummod	one,complex
R6284	T9394	T9389	nsubj	complex,is
R6285	T9395	T9394	nmod	Mcm4,complex
R6286	T9396	T9395	punct	/,Mcm4
R6287	T9397	T9395	nummod	6,Mcm4
R6288	T9398	T9395	punct	/,Mcm4
R6289	T9399	T9395	nummod	7,Mcm4
R6290	T9400	T9389	acomp	likely,is
R6291	T9401	T9402	aux	to,encircle
R6292	T9402	T9400	xcomp	encircle,likely
R6293	T9403	T9404	det	the,strand
R6294	T9404	T9402	dobj	strand,encircle
R6295	T9405	T9406	amod	single,stranded
R6296	T9406	T9404	amod	stranded,strand
R6297	T9407	T9406	punct	-,stranded
R6298	T9408	T9404	compound	DNA,strand
R6299	T9409	T9389	cc	and,is
R6300	T9410	T9411	nummod	two,hexamers
R6301	T9411	T9412	nsubj	hexamers,bind
R6302	T9412	T9389	conj	bind,is
R6303	T9413	T9412	aux	may,bind
R6304	T9414	T9412	advmod	symmetrically,bind
R6305	T9415	T9412	prep	to,bind
R6306	T9416	T9417	det	the,substrate
R6307	T9417	T9415	pobj	substrate,to
R6308	T9418	T9417	compound	bubble,substrate
R6309	T9419	T9412	punct	", ",bind
R6310	T9420	T9412	advcl	forming,bind
R6311	T9421	T9422	det	a,structure
R6312	T9422	T9420	dobj	structure,forming
R6313	T9423	T9424	amod	double,hexameric
R6314	T9424	T9422	amod	hexameric,structure
R6315	T9425	T9420	prep	on,forming
R6316	T9426	T9427	det	the,bubble
R6317	T9427	T9425	pobj	bubble,on
R6318	T9428	T9429	punct	(,see
R6319	T9429	T9420	parataxis	see,forming
R6320	T9430	T9431	amod	schematic,drawing
R6321	T9431	T9429	dobj	drawing,see
R6322	T9432	T9431	prep	of,drawing
R6323	T9433	T9434	compound	Figure,1A
R6324	T9434	T9432	pobj	1A,of
R6325	T9435	T9429	punct	),see
R6326	T9436	T9412	punct	.,bind
R6327	T9438	T9439	amod	Efficient,unwinding
R6328	T9439	T9440	nsubj	unwinding,require
R6329	T9441	T9439	prep	into,unwinding
R6330	T9442	T9443	det	both,directions
R6331	T9443	T9441	pobj	directions,into
R6332	T9444	T9440	aux	may,require
R6333	T9445	T9446	amod	simultaneous,activation
R6334	T9446	T9440	dobj	activation,require
R6335	T9447	T9446	prep	of,activation
R6336	T9448	T9449	det	both,hexamers
R6337	T9449	T9447	pobj	hexamers,of
R6338	T9450	T9451	dep	which,sit
R6339	T9451	T9449	relcl	sit,hexamers
R6340	T9452	T9451	aux	may,sit
R6341	T9453	T9451	prep	at,sit
R6342	T9454	T9455	det	the,center
R6343	T9455	T9453	pobj	center,at
R6344	T9456	T9457	mark	while,extruding
R6345	T9457	T9451	advcl	extruding,sit
R6346	T9458	T9459	det	the,DNA
R6347	T9459	T9457	dobj	DNA,extruding
R6348	T9460	T9459	amod	unwound,DNA
R6349	T9461	T9462	amod	single,stranded
R6350	T9462	T9459	amod	stranded,DNA
R6351	T9463	T9462	punct	-,stranded
R6352	T9464	T9457	prep	through,extruding
R6353	T9465	T9466	det	the,rings
R6354	T9466	T9464	pobj	rings,through
R6355	T9467	T9440	punct	.,require
R6356	T9469	T9470	nsubj	This,resemble
R6357	T9471	T9470	aux	may,resemble
R6358	T9472	T9470	advmod	closely,resemble
R6359	T9473	T9474	det	the,modes
R6360	T9474	T9470	dobj	modes,resemble
R6361	T9475	T9474	amod	proposed,modes
R6362	T9476	T9474	prep	of,modes
R6363	T9477	T9478	nmod	binding,actions
R6364	T9478	T9476	pobj	actions,of
R6365	T9479	T9477	cc	and,binding
R6366	T9480	T9477	conj	helicase,binding
R6367	T9481	T9478	prep	of,actions
R6368	T9482	T9483	compound	T,antigen
R6369	T9483	T9481	pobj	antigen,of
R6370	T9484	T9483	punct	-,antigen
R6371	T9485	T9486	punct	(,21
R6372	T9486	T9470	parataxis	21,resemble
R6373	T9487	T9486	punct	),21
R6374	T9488	T9470	punct	.,resemble
R6375	T9490	T9491	mark	Although,exclude
R6376	T9491	T9496	advcl	exclude,bear
R6377	T9492	T9491	nsubj	we,exclude
R6378	T9493	T9491	aux	can,exclude
R6379	T9494	T9491	neg	not,exclude
R6380	T9495	T9491	advmod	totally,exclude
R6381	T9497	T9498	det	the,possibility
R6382	T9498	T9491	dobj	possibility,exclude
R6383	T9499	T9500	mark	that,unwinds
R6384	T9500	T9498	acl	unwinds,possibility
R6385	T9501	T9502	nummod	one,hexamer
R6386	T9502	T9500	nsubj	hexamer,unwinds
R6387	T9503	T9502	nmod	Mcm4,hexamer
R6388	T9504	T9503	punct	/,Mcm4
R6389	T9505	T9503	nummod	6,Mcm4
R6390	T9506	T9503	punct	/,Mcm4
R6391	T9507	T9503	nummod	7,Mcm4
R6392	T9508	T9502	prep	at,hexamer
R6393	T9509	T9510	det	each,fork
R6394	T9510	T9508	pobj	fork,at
R6395	T9511	T9512	det	the,duplex
R6396	T9512	T9500	dobj	duplex,unwinds
R6397	T9513	T9500	advmod	independently,unwinds
R6398	T9514	T9496	punct	", ",bear
R6399	T9515	T9516	npadvmod	ring,shaped
R6400	T9516	T9518	amod	shaped,structures
R6401	T9517	T9516	punct	-,shaped
R6402	T9518	T9496	nsubj	structures,bear
R6403	T9519	T9518	prep	of,structures
R6404	T9520	T9521	nmod	mouse,MCM
R6405	T9521	T9519	pobj	MCM,of
R6406	T9522	T9520	cc	and,mouse
R6407	T9523	T9520	conj	archea,mouse
R6408	T9524	T9496	punct	", ",bear
R6409	T9525	T9526	mark	as,revealed
R6410	T9526	T9496	advcl	revealed,bear
R6411	T9527	T9526	agent	by,revealed
R6412	T9528	T9529	compound	electron,microscopy
R6413	T9529	T9527	pobj	microscopy,by
R6414	T9530	T9496	punct	", ",bear
R6415	T9531	T9532	amod	much,similarity
R6416	T9532	T9496	dobj	similarity,bear
R6417	T9533	T9532	prep	to,similarity
R6418	T9534	T9535	det	the,structure
R6419	T9535	T9533	pobj	structure,to
R6420	T9536	T9537	advmod	recently,solved
R6421	T9537	T9535	amod	solved,structure
R6422	T9538	T9535	prep	of,structure
R6423	T9539	T9540	det	the,antigen
R6424	T9540	T9538	pobj	antigen,of
R6425	T9541	T9540	nmod	SV40,antigen
R6426	T9542	T9540	amod	large,antigen
R6427	T9543	T9540	compound	T,antigen
R6428	T9544	T9540	punct	-,antigen
R6429	T9545	T9546	punct	(,6
R6430	T9546	T9535	parataxis	6,structure
R6431	T9547	T9546	nummod	4,6
R6432	T9548	T9546	punct	",",6
R6433	T9549	T9546	punct	),6
R6434	T9550	T9496	punct	", ",bear
R6435	T9551	T9496	cc	and,bear
R6436	T9552	T9496	conj	are,bear
R6437	T9553	T9552	prep	in,are
R6438	T9554	T9553	pobj	favor,in
R6439	T9555	T9554	prep	of,favor
R6440	T9556	T9557	det	the,structure
R6441	T9557	T9555	pobj	structure,of
R6442	T9558	T9559	amod	double,hexameric
R6443	T9559	T9557	amod	hexameric,structure
R6444	T9560	T9559	punct	-,hexameric
R6445	T9561	T9557	prep	of,structure
R6446	T9562	T9563	compound	mouse,Mcm
R6447	T9563	T9561	pobj	Mcm,of
R6448	T9564	T9557	prep	on,structure
R6449	T9565	T9566	det	a,DNA
R6450	T9566	T9564	pobj	DNA,on
R6451	T9567	T9566	compound	bubble,DNA
R6452	T9568	T9496	punct	.,bear
R6455	T9856	T9857	nmod	Substrate,requirement
R6456	T9858	T9856	cc	and,Substrate
R6457	T9859	T9856	conj	sequence,Substrate
R6458	T9860	T9857	prep	for,requirement
R6459	T9861	T9862	nmod	Mcm4,activation
R6460	T9862	T9860	pobj	activation,for
R6461	T9863	T9861	punct	/,Mcm4
R6462	T9864	T9861	nummod	6,Mcm4
R6463	T9865	T9861	punct	/,Mcm4
R6464	T9866	T9861	nummod	7,Mcm4
R6465	T9867	T9862	compound	helicase,activation
R6466	T9869	T9870	compound	DNA,binding
R6467	T9870	T9871	compound	binding,assays
R6468	T9871	T9872	nsubj	assays,indicate
R6469	T9873	T9874	mark	that,binds
R6470	T9874	T9872	ccomp	binds,indicate
R6471	T9875	T9874	nsubj	Mcm4,binds
R6472	T9876	T9875	punct	/,Mcm4
R6473	T9877	T9875	nummod	6,Mcm4
R6474	T9878	T9875	punct	/,Mcm4
R6475	T9879	T9875	nummod	7,Mcm4
R6476	T9880	T9874	prep	to,binds
R6477	T9881	T9882	det	those,substrates
R6478	T9882	T9880	pobj	substrates,to
R6479	T9883	T9882	acl	containing,substrates
R6480	T9884	T9885	amod	single,stranded
R6481	T9885	T9887	amod	stranded,regions
R6482	T9886	T9885	punct	-,stranded
R6483	T9887	T9883	dobj	regions,containing
R6484	T9888	T9887	compound	DNA,regions
R6485	T9889	T9874	advmod	regardless,binds
R6486	T9890	T9889	prep	of,regardless
R6487	T9891	T9892	det	the,presence
R6488	T9892	T9890	pobj	presence,of
R6489	T9893	T9892	cc	or,presence
R6490	T9894	T9892	conj	absence,presence
R6491	T9895	T9892	prep	of,presence
R6492	T9896	T9897	amod	single,stranded
R6493	T9897	T9899	amod	stranded,end
R6494	T9898	T9897	punct	-,stranded
R6495	T9899	T9895	pobj	end,of
R6496	T9900	T9899	nummod	3,end
R6497	T9901	T9900	punct	′,3
R6498	T9902	T9900	cc	or,3
R6499	T9903	T9900	conj	5,3
R6500	T9904	T9903	punct	′,5
R6501	T9905	T9872	punct	.,indicate
R6502	T9907	T9908	nsubj	Unwinding,depends
R6503	T9909	T9907	prep	of,Unwinding
R6504	T9910	T9911	det	the,DNA
R6505	T9911	T9909	pobj	DNA,of
R6506	T9912	T9911	compound	duplex,DNA
R6507	T9913	T9908	prep	on,depends
R6508	T9914	T9913	pobj	translocation,on
R6509	T9915	T9914	prep	of,translocation
R6510	T9916	T9917	amod	single,stranded
R6511	T9917	T9919	amod	stranded,DNA
R6512	T9918	T9917	punct	-,stranded
R6513	T9919	T9915	pobj	DNA,of
R6514	T9920	T9914	prep	from,translocation
R6515	T9921	T9920	pobj	3,from
R6516	T9922	T9921	punct	′,3
R6517	T9923	T9920	prep	to,from
R6518	T9924	T9923	pobj	5,to
R6519	T9925	T9924	punct	′,5
R6520	T9926	T9920	pobj	direction,from
R6521	T9927	T9908	punct	.,depends
R6522	T9929	T9930	nsubjpass	This,shown
R6523	T9931	T9930	auxpass	is,shown
R6524	T9932	T9933	advmod	most,clearly
R6525	T9933	T9930	advmod	clearly,shown
R6526	T9934	T9930	prep	by,shown
R6527	T9935	T9936	poss	its,action
R6528	T9936	T9934	pobj	action,by
R6529	T9937	T9936	compound	helicase,action
R6530	T9938	T9936	prep	on,action
R6531	T9939	T9940	nmod	A,fork
R6532	T9940	T9942	nmod	fork,extension
R6533	T9941	T9940	punct	-,fork
R6534	T9942	T9938	pobj	extension,on
R6535	T9943	T9940	punct	[,fork
R6536	T9944	T9940	appos	5,fork
R6537	T9945	T9944	punct	′,5
R6538	T9946	T9944	punct	],5
R6539	T9947	T9944	cc	and,5
R6540	T9948	T9944	conj	3,5
R6541	T9949	T9948	punct	′,3
R6542	T9950	T9942	punct	-,extension
R6543	T9951	T9938	cc	but,on
R6544	T9952	T9951	neg	not,but
R6545	T9953	T9938	conj	on,on
R6546	T9954	T9955	nmod	A,fork
R6547	T9955	T9957	nmod	fork,extension
R6548	T9956	T9955	punct	-,fork
R6549	T9957	T9953	pobj	extension,on
R6550	T9958	T9955	punct	[,fork
R6551	T9959	T9955	nummod	3,fork
R6552	T9960	T9955	punct	′,fork
R6553	T9961	T9955	punct	],fork
R6554	T9962	T9955	punct	", ",fork
R6555	T9963	T9964	compound	A,fork
R6556	T9964	T9955	conj	fork,fork
R6557	T9965	T9964	punct	-,fork
R6558	T9966	T9964	punct	[,fork
R6559	T9967	T9964	appos	3,fork
R6560	T9968	T9967	punct	′,3
R6561	T9969	T9967	punct	",",3
R6562	T9970	T9967	appos	5,3
R6563	T9971	T9970	punct	′,5
R6564	T9972	T9964	punct	],fork
R6565	T9973	T9964	cc	nor,fork
R6566	T9974	T9964	conj	5,fork
R6567	T9975	T9974	punct	′,5
R6568	T9976	T9957	punct	-,extension
R6569	T9977	T9930	punct	.,shown
R6570	T9979	T9980	advmod	However,require
R6571	T9981	T9980	punct	", ",require
R6572	T9982	T9980	nsubj	it,require
R6573	T9983	T9980	aux	does,require
R6574	T9984	T9980	neg	not,require
R6575	T9985	T9980	advmod	necessarily,require
R6576	T9986	T9987	nummod	3,end
R6577	T9987	T9980	dobj	end,require
R6578	T9988	T9986	punct	′,3
R6579	T9989	T9987	prep	of,end
R6580	T9990	T9991	amod	single,stranded
R6581	T9991	T9993	amod	stranded,DNA
R6582	T9992	T9991	punct	-,stranded
R6583	T9993	T9989	pobj	DNA,of
R6584	T9994	T9980	punct	", ",require
R6585	T9995	T9996	mark	since,displace
R6586	T9996	T9980	advcl	displace,require
R6587	T9997	T9996	nsubj	Mcm4,displace
R6588	T9998	T9997	punct	/,Mcm4
R6589	T9999	T9997	nummod	6,Mcm4
R6590	T10000	T9997	punct	/,Mcm4
R6591	T10001	T9997	nummod	7,Mcm4
R6592	T10002	T9996	aux	can,displace
R6593	T10003	T10004	amod	annealing,oligonucleotide
R6594	T10004	T9996	dobj	oligonucleotide,displace
R6595	T10005	T9996	prep	on,displace
R6596	T10006	T10007	det	a,DNA
R6597	T10007	T10005	pobj	DNA,on
R6598	T10008	T10007	amod	circular,DNA
R6599	T10009	T10010	amod	single,stranded
R6600	T10010	T10007	amod	stranded,DNA
R6601	T10011	T10010	punct	-,stranded
R6602	T10012	T9980	punct	.,require
R6603	T10014	T10015	det	The,ability
R6604	T10015	T10016	nsubjpass	ability,shared
R6605	T10017	T10015	prep	of,ability
R6606	T10018	T10019	det	the,Mcm4
R6607	T10019	T10017	pobj	Mcm4,of
R6608	T10020	T10019	compound	mouse,Mcm4
R6609	T10021	T10019	punct	/,Mcm4
R6610	T10022	T10019	nummod	6,Mcm4
R6611	T10023	T10019	punct	/,Mcm4
R6612	T10024	T10019	nummod	7,Mcm4
R6613	T10025	T10026	aux	to,unwind
R6614	T10026	T10015	acl	unwind,ability
R6615	T10027	T10028	nummod	3,extension
R6616	T10028	T10026	dobj	extension,unwind
R6617	T10029	T10027	punct	′,3
R6618	T10030	T10028	punct	-,extension
R6619	T10031	T10016	auxpass	is,shared
R6620	T10032	T10016	prep	by,shared
R6621	T10033	T10034	det	the,helicase
R6622	T10034	T10032	pobj	helicase,by
R6623	T10035	T10034	amod	archaeal,helicase
R6624	T10036	T10034	compound	Mcm,helicase
R6625	T10037	T10032	cc	but,by
R6626	T10038	T10037	neg	not,but
R6627	T10039	T10032	conj	by,by
R6628	T10040	T10039	pobj	Mcm4,by
R6629	T10041	T10040	punct	/,Mcm4
R6630	T10042	T10040	nummod	6,Mcm4
R6631	T10043	T10040	punct	/,Mcm4
R6632	T10044	T10040	nummod	7,Mcm4
R6633	T10045	T10040	prep	from,Mcm4
R6634	T10046	T10045	pobj	S.pombe,from
R6635	T10047	T10046	cc	and,S.pombe
R6636	T10048	T10046	conj	S.cerevisiae,S.pombe
R6637	T10049	T10050	punct	(,27
R6638	T10050	T10016	parataxis	27,shared
R6639	T10051	T10050	nummod	14,27
R6640	T10052	T10050	punct	",",27
R6641	T10053	T10050	nummod	26,27
R6642	T10054	T10050	punct	",",27
R6643	T10055	T10050	punct	),27
R6644	T10056	T10016	punct	.,shared
R6645	T10058	T10059	det	The,Mcm
R6646	T10059	T10061	nsubj	Mcm,unwind
R6647	T10060	T10059	amod	archaeal,Mcm
R6648	T10062	T10061	aux	can,unwind
R6649	T10063	T10064	compound	A,fork
R6650	T10064	T10061	dobj	fork,unwind
R6651	T10065	T10064	punct	-,fork
R6652	T10066	T10064	punct	[,fork
R6653	T10067	T10064	nummod	3,fork
R6654	T10068	T10064	punct	′,fork
R6655	T10069	T10061	punct	],unwind
R6656	T10070	T10061	cc	but,unwind
R6657	T10071	T10072	amod	eukaryotic,Mcm4
R6658	T10072	T10073	nsubj	Mcm4,can
R6659	T10073	T10061	conj	can,unwind
R6660	T10074	T10072	punct	/,Mcm4
R6661	T10075	T10072	nummod	6,Mcm4
R6662	T10076	T10072	punct	/,Mcm4
R6663	T10077	T10072	nummod	7,Mcm4
R6664	T10078	T10073	neg	not,can
R6665	T10079	T10061	punct	", ",unwind
R6666	T10080	T10081	mark	since,binds
R6667	T10081	T10061	advcl	binds,unwind
R6668	T10082	T10083	det	the,former
R6669	T10083	T10081	nsubj	former,binds
R6670	T10084	T10081	prep	to,binds
R6671	T10085	T10086	amod	double,stranded
R6672	T10086	T10088	amod	stranded,DNA
R6673	T10087	T10086	punct	-,stranded
R6674	T10088	T10084	pobj	DNA,to
R6675	T10089	T10081	cc	but,binds
R6676	T10090	T10091	det	the,latter
R6677	T10091	T10092	nsubj	latter,27
R6678	T10092	T10081	conj	27,binds
R6679	T10093	T10092	aux	does,27
R6680	T10094	T10092	neg	not,27
R6681	T10095	T10092	punct	(,27
R6682	T10096	T10092	nummod	5,27
R6683	T10097	T10092	punct	",",27
R6684	T10098	T10092	punct	),27
R6685	T10099	T10061	punct	.,unwind
R6686	T10101	T10102	mark	While,translocate
R6687	T10102	T10107	advcl	translocate,observed
R6688	T10103	T10104	compound	yeast,Mcm
R6689	T10104	T10105	compound	Mcm,helicases
R6690	T10105	T10102	nsubj	helicases,translocate
R6691	T10106	T10102	aux	can,translocate
R6692	T10108	T10102	prep	on,translocate
R6693	T10109	T10110	compound	duplex,DNA
R6694	T10110	T10108	pobj	DNA,on
R6695	T10111	T10107	punct	", ",observed
R6696	T10112	T10113	amod	such,activity
R6697	T10113	T10107	nsubjpass	activity,observed
R6698	T10114	T10107	auxpass	was,observed
R6699	T10115	T10107	neg	not,observed
R6700	T10116	T10107	prep	with,observed
R6701	T10117	T10118	amod	mammalian,Mcm4
R6702	T10118	T10116	pobj	Mcm4,with
R6703	T10119	T10118	punct	/,Mcm4
R6704	T10120	T10118	nummod	6,Mcm4
R6705	T10121	T10118	punct	/,Mcm4
R6706	T10122	T10118	nummod	7,Mcm4
R6707	T10123	T10124	punct	(,shown
R6708	T10124	T10107	parataxis	shown,observed
R6709	T10125	T10124	nsubj	data,shown
R6710	T10126	T10124	neg	not,shown
R6711	T10127	T10124	punct	),shown
R6712	T10128	T10107	punct	.,observed
R6713	T10130	T10131	nsubj	Occurrence,lead
R6714	T10132	T10130	prep	of,Occurrence
R6715	T10133	T10134	npadvmod	AT,rich
R6716	T10134	T10136	amod	rich,sequences
R6717	T10135	T10134	punct	-,rich
R6718	T10136	T10132	pobj	sequences,of
R6719	T10137	T10130	punct	", ",Occurrence
R6720	T10138	T10130	prep	with,Occurrence
R6721	T10139	T10140	amod	asymmetric,distribution
R6722	T10140	T10138	pobj	distribution,with
R6723	T10141	T10140	prep	of,distribution
R6724	T10142	T10141	pobj	adenine,of
R6725	T10143	T10142	cc	and,adenine
R6726	T10144	T10142	conj	thymine,adenine
R6727	T10145	T10140	punct	", ",distribution
R6728	T10146	T10140	prep	near,distribution
R6729	T10147	T10148	det	the,origins
R6730	T10148	T10146	pobj	origins,near
R6731	T10149	T10148	compound	replication,origins
R6732	T10150	T10131	punct	", ",lead
R6733	T10151	T10131	dobj	us,lead
R6734	T10152	T10153	aux	to,propose
R6735	T10153	T10131	xcomp	propose,lead
R6736	T10154	T10155	mark	that,play
R6737	T10155	T10153	ccomp	play,propose
R6738	T10156	T10155	nsubj	Mcm,play
R6739	T10157	T10155	aux	may,play
R6740	T10158	T10159	det	a,role
R6741	T10159	T10155	dobj	role,play
R6742	T10160	T10155	prep	in,play
R6743	T10161	T10160	pobj	selection,in
R6744	T10162	T10161	prep	of,selection
R6745	T10163	T10164	compound	initiation,sites
R6746	T10164	T10162	pobj	sites,of
R6747	T10165	T10164	prep	of,sites
R6748	T10166	T10167	amod	mammalian,DNA
R6749	T10167	T10168	compound	DNA,replication
R6750	T10168	T10165	pobj	replication,of
R6751	T10169	T10131	punct	", ",lead
R6752	T10170	T10131	cc	and,lead
R6753	T10171	T10131	conj	prompted,lead
R6754	T10172	T10171	dobj	us,prompted
R6755	T10173	T10174	aux	to,examine
R6756	T10174	T10171	xcomp	examine,prompted
R6757	T10175	T10176	det	the,ability
R6758	T10176	T10174	dobj	ability,examine
R6759	T10177	T10176	prep	of,ability
R6760	T10178	T10177	pobj	sequences,of
R6761	T10179	T10178	prep	from,sequences
R6762	T10180	T10181	amod	human,origins
R6763	T10181	T10179	pobj	origins,from
R6764	T10182	T10181	compound	replication,origins
R6765	T10183	T10184	aux	to,activate
R6766	T10184	T10176	acl	activate,ability
R6767	T10185	T10186	nmod	Mcm4,helicase
R6768	T10186	T10184	dobj	helicase,activate
R6769	T10187	T10185	punct	/,Mcm4
R6770	T10188	T10185	nummod	6,Mcm4
R6771	T10189	T10185	punct	/,Mcm4
R6772	T10190	T10185	nummod	7,Mcm4
R6773	T10191	T10131	punct	.,lead
R6774	T10193	T10194	preconj	Both,B2
R6775	T10194	T10197	nmod	B2,origins
R6776	T10195	T10194	nmod	lamin,B2
R6777	T10196	T10194	punct	-,B2
R6778	T10197	T10202	nsubj	origins,served
R6779	T10198	T10194	cc	and,B2
R6780	T10199	T10200	compound	c,myc
R6781	T10200	T10194	conj	myc,B2
R6782	T10201	T10200	punct	-,myc
R6783	T10203	T10202	prep	as,served
R6784	T10204	T10205	amod	efficient,activator
R6785	T10205	T10203	pobj	activator,as
R6786	T10206	T10205	prep	for,activator
R6787	T10207	T10208	compound	Mcm,helicase
R6788	T10208	T10206	pobj	helicase,for
R6789	T10209	T10210	advmod	in,vitro
R6790	T10210	T10202	advmod	vitro,served
R6791	T10211	T10202	punct	.,served
R6792	T10213	T10214	advcl	Consistent,reported
R6793	T10215	T10213	prep	with,Consistent
R6794	T10216	T10215	pobj	it,with
R6795	T10217	T10214	punct	", ",reported
R6796	T10218	T10219	npadvmod	site,specific
R6797	T10219	T10221	amod	specific,loading
R6798	T10220	T10219	punct	-,specific
R6799	T10221	T10214	nsubjpass	loading,reported
R6800	T10222	T10221	prep	of,loading
R6801	T10223	T10222	pobj	Mcm,of
R6802	T10224	T10221	prep	in,loading
R6803	T10225	T10226	det	the,zone
R6804	T10226	T10224	pobj	zone,in
R6805	T10227	T10228	compound	DNA,initiation
R6806	T10228	T10226	compound	initiation,zone
R6807	T10229	T10228	compound	replication,initiation
R6808	T10230	T10226	prep	of,zone
R6809	T10231	T10232	det	the,myc
R6810	T10232	T10230	pobj	myc,of
R6811	T10233	T10232	compound	c,myc
R6812	T10234	T10232	punct	-,myc
R6813	T10235	T10214	auxpass	was,reported
R6814	T10236	T10214	advmod	recently,reported
R6815	T10237	T10238	punct	(,33
R6816	T10238	T10214	parataxis	33,reported
R6817	T10239	T10238	punct	),33
R6818	T10240	T10214	punct	.,reported
R6819	T10242	T10243	nsubj	We,examined
R6820	T10244	T10243	aux	have,examined
R6821	T10245	T10243	prep	in,examined
R6822	T10246	T10245	pobj	detail,in
R6823	T10247	T10248	det	the,effect
R6824	T10248	T10243	dobj	effect,examined
R6825	T10249	T10248	prep	of,effect
R6826	T10250	T10251	compound	sequence,context
R6827	T10251	T10249	pobj	context,of
R6828	T10252	T10251	prep	of,context
R6829	T10253	T10254	det	the,DNA
R6830	T10254	T10252	pobj	DNA,of
R6831	T10255	T10256	amod	single,stranded
R6832	T10256	T10254	amod	stranded,DNA
R6833	T10257	T10256	punct	-,stranded
R6834	T10258	T10248	prep	on,effect
R6835	T10259	T10260	det	the,activity
R6836	T10260	T10258	pobj	activity,on
R6837	T10261	T10260	compound	helicase,activity
R6838	T10262	T10260	prep	of,activity
R6839	T10263	T10262	pobj	Mcm,of
R6840	T10264	T10243	punct	.,examined
R6841	T10266	T10267	det	The,results
R6842	T10267	T10268	nsubj	results,indicate
R6843	T10269	T10270	mark	that,is
R6844	T10270	T10268	ccomp	is,indicate
R6845	T10271	T10272	compound	thymine,content
R6846	T10272	T10270	nsubj	content,is
R6847	T10273	T10272	prep	of,content
R6848	T10274	T10275	nummod	50,%
R6849	T10275	T10273	pobj	%,of
R6850	T10276	T10270	acomp	sufficient,is
R6851	T10277	T10276	prep	for,sufficient
R6852	T10278	T10279	det	the,activity
R6853	T10279	T10277	pobj	activity,for
R6854	T10280	T10279	amod	maximum,activity
R6855	T10281	T10279	compound	helicase,activity
R6856	T10282	T10268	punct	.,indicate
R6857	T10284	T10285	det	The,efficiency
R6858	T10285	T10286	nsubj	efficiency,decreased
R6859	T10287	T10285	prep	of,efficiency
R6860	T10288	T10287	pobj	displacement,of
R6861	T10289	T10290	mark	as,dropped
R6862	T10290	T10286	advcl	dropped,decreased
R6863	T10291	T10292	det	the,content
R6864	T10292	T10290	nsubj	content,dropped
R6865	T10293	T10292	compound	thymine,content
R6866	T10294	T10292	prep	of,content
R6867	T10295	T10296	det	the,tail
R6868	T10296	T10294	pobj	tail,of
R6869	T10297	T10296	nummod	3,tail
R6870	T10298	T10297	punct	′,3
R6871	T10299	T10296	punct	-,tail
R6872	T10300	T10290	prep	to,dropped
R6873	T10301	T10302	nummod	33,%
R6874	T10302	T10300	pobj	%,to
R6875	T10303	T10304	punct	(,Figure
R6876	T10304	T10290	parataxis	Figure,dropped
R6877	T10305	T10304	nummod	6,Figure
R6878	T10306	T10304	punct	),Figure
R6879	T10307	T10286	punct	.,decreased
R6880	T10309	T10310	det	The,stretches
R6881	T10310	T10311	nsubjpass	stretches,required
R6882	T10312	T10310	prep	of,stretches
R6883	T10313	T10314	compound	thymine,residues
R6884	T10314	T10312	pobj	residues,of
R6885	T10315	T10311	aux	may,required
R6886	T10316	T10311	neg	not,required
R6887	T10317	T10311	auxpass	be,required
R6888	T10318	T10311	advmod	necessarily,required
R6889	T10319	T10311	punct	", ",required
R6890	T10320	T10321	mark	since,served
R6891	T10321	T10311	advcl	served,required
R6892	T10322	T10321	nsubj	repeats,served
R6893	T10323	T10322	prep	of,repeats
R6894	T10324	T10325	compound	TC,dinucleotides
R6895	T10325	T10323	pobj	dinucleotides,of
R6896	T10326	T10321	prep	as,served
R6897	T10327	T10328	det	a,activator
R6898	T10328	T10326	pobj	activator,as
R6899	T10329	T10328	amod	potent,activator
R6900	T10330	T10328	prep	for,activator
R6901	T10331	T10330	pobj	Mcm4,for
R6902	T10332	T10331	punct	/,Mcm4
R6903	T10333	T10331	nummod	6,Mcm4
R6904	T10334	T10331	punct	/,Mcm4
R6905	T10335	T10331	nummod	7,Mcm4
R6906	T10336	T10311	punct	.,required
R6907	T10338	T10339	nsubj	We,noticed
R6908	T10340	T10339	advmod	also,noticed
R6909	T10341	T10342	mark	that,is
R6910	T10342	T10339	ccomp	is,noticed
R6911	T10343	T10344	det	the,presence
R6912	T10344	T10342	nsubj	presence,is
R6913	T10345	T10344	prep	of,presence
R6914	T10346	T10347	det	a,structure
R6915	T10347	T10345	pobj	structure,of
R6916	T10348	T10347	amod	secondary,structure
R6917	T10349	T10347	prep	within,structure
R6918	T10350	T10351	det	the,DNA
R6919	T10351	T10349	pobj	DNA,within
R6920	T10352	T10353	amod	single,stranded
R6921	T10353	T10351	amod	stranded,DNA
R6922	T10354	T10353	punct	-,stranded
R6923	T10355	T10342	acomp	inhibitory,is
R6924	T10356	T10355	prep	for,inhibitory
R6925	T10357	T10358	compound	helicase,action
R6926	T10358	T10356	pobj	action,for
R6927	T10359	T10339	punct	.,noticed
R6928	T10361	T10362	compound	Nuclease,footprinting
R6929	T10362	T10363	compound	footprinting,assays
R6930	T10363	T10364	nsubj	assays,indicated
R6931	T10365	T10366	mark	that,interfered
R6932	T10366	T10364	ccomp	interfered,indicated
R6933	T10367	T10366	nsubjpass	binding,interfered
R6934	T10368	T10366	auxpass	was,interfered
R6935	T10369	T10366	agent	by,interfered
R6936	T10370	T10371	det	the,structure
R6937	T10371	T10369	pobj	structure,by
R6938	T10372	T10371	amod	secondary,structure
R6939	T10373	T10374	punct	(,shown
R6940	T10374	T10366	parataxis	shown,interfered
R6941	T10375	T10374	nsubj	data,shown
R6942	T10376	T10374	neg	not,shown
R6943	T10377	T10374	punct	),shown
R6944	T10378	T10364	punct	.,indicated
R6945	T10380	T10381	advmod	Thus,concluded
R6946	T10382	T10381	punct	", ",concluded
R6947	T10383	T10381	nsubj	we,concluded
R6948	T10384	T10381	aux	have,concluded
R6949	T10385	T10386	mark	that,activated
R6950	T10386	T10381	ccomp	activated,concluded
R6951	T10387	T10388	nmod	Mcm4,helicase
R6952	T10388	T10386	nsubjpass	helicase,activated
R6953	T10389	T10387	punct	/,Mcm4
R6954	T10390	T10387	nummod	6,Mcm4
R6955	T10391	T10387	punct	/,Mcm4
R6956	T10392	T10387	nummod	7,Mcm4
R6957	T10393	T10386	auxpass	is,activated
R6958	T10394	T10395	advmod	most,efficiently
R6959	T10395	T10386	advmod	efficiently,activated
R6960	T10396	T10386	agent	by,activated
R6961	T10397	T10398	amod	non-structured,DNA
R6962	T10398	T10396	pobj	DNA,by
R6963	T10399	T10400	amod	single,stranded
R6964	T10400	T10398	amod	stranded,DNA
R6965	T10401	T10400	punct	-,stranded
R6966	T10402	T10398	prep	with,DNA
R6967	T10403	T10404	compound	thymine,content
R6968	T10404	T10402	pobj	content,with
R6969	T10405	T10404	prep	of,content
R6970	T10406	T10407	nummod	50,%
R6971	T10407	T10405	pobj	%,of
R6972	T10408	T10409	cc	or,more
R6973	T10409	T10407	nummod	more,%
R6974	T10410	T10386	punct	", ",activated
R6975	T10411	T10412	mark	although,observed
R6976	T10412	T10386	advcl	observed,activated
R6977	T10413	T10414	amod	significant,stimulation
R6978	T10414	T10412	nsubjpass	stimulation,observed
R6979	T10415	T10412	auxpass	is,observed
R6980	T10416	T10412	advmod	also,observed
R6981	T10417	T10412	agent	by,observed
R6982	T10418	T10417	pobj	DNA,by
R6983	T10419	T10418	prep	with,DNA
R6984	T10420	T10421	amod	less,content
R6985	T10421	T10419	pobj	content,with
R6986	T10422	T10421	compound	thymine,content
R6987	T10423	T10424	punct	(,Figure
R6988	T10424	T10418	parataxis	Figure,DNA
R6989	T10425	T10424	nummod	7,Figure
R6990	T10426	T10424	punct	),Figure
R6991	T10427	T10412	punct	", ",observed
R6992	T10428	T10412	advcl	suggesting,observed
R6993	T10429	T10430	mark	that,is
R6994	T10430	T10428	advcl	is,suggesting
R6995	T10431	T10432	det	the,specificity
R6996	T10432	T10430	nsubj	specificity,is
R6997	T10433	T10432	compound	sequence,specificity
R6998	T10434	T10432	prep	for,specificity
R6999	T10435	T10436	compound	Mcm,helicase
R7000	T10436	T10437	compound	helicase,activation
R7001	T10437	T10434	pobj	activation,for
R7002	T10438	T10439	advmod	rather,relaxed
R7003	T10439	T10430	acomp	relaxed,is
R7004	T10440	T10430	cc	and,is
R7005	T10441	T10442	mark	that,depend
R7006	T10442	T10430	conj	depend,is
R7007	T10443	T10444	det	the,extent
R7008	T10444	T10442	nsubj	extent,depend
R7009	T10445	T10444	prep	of,extent
R7010	T10446	T10447	det	the,activation
R7011	T10447	T10445	pobj	activation,of
R7012	T10448	T10442	aux	may,depend
R7013	T10449	T10442	prep	on,depend
R7014	T10450	T10451	det	the,context
R7015	T10451	T10449	pobj	context,on
R7016	T10452	T10451	compound	sequence,context
R7017	T10453	T10381	punct	.,concluded
R7018	T10455	T10456	nsubj	This,be
R7019	T10457	T10456	aux	would,be
R7020	T10458	T10456	acomp	reasonable,be
R7021	T10459	T10456	prep	given,be
R7022	T10460	T10461	det	the,flexibility
R7023	T10461	T10459	pobj	flexibility,given
R7024	T10462	T10461	cc	and,flexibility
R7025	T10463	T10464	amod	differential,regulation
R7026	T10464	T10461	conj	regulation,flexibility
R7027	T10465	T10461	prep	of,flexibility
R7028	T10466	T10467	compound	site,selection
R7029	T10467	T10465	pobj	selection,of
R7030	T10468	T10461	prep	for,flexibility
R7031	T10469	T10468	pobj	initiation,for
R7032	T10470	T10469	prep	of,initiation
R7033	T10471	T10472	compound	DNA,replication
R7034	T10472	T10470	pobj	replication,of
R7035	T10473	T10469	prep	during,initiation
R7036	T10474	T10473	pobj	development,during
R7037	T10475	T10468	cc	or,for
R7038	T10476	T10468	conj	in,for
R7039	T10477	T10478	amod	various,types
R7040	T10478	T10476	pobj	types,in
R7041	T10479	T10478	compound	cell,types
R7042	T10480	T10461	punct	", ",flexibility
R7043	T10481	T10482	advmod	as,as
R7044	T10482	T10461	cc	as,flexibility
R7045	T10483	T10482	advmod	well,as
R7046	T10484	T10485	det	the,variability
R7047	T10485	T10461	conj	variability,flexibility
R7048	T10486	T10485	prep	in,variability
R7049	T10487	T10488	compound	initiation,potential
R7050	T10488	T10486	pobj	potential,in
R7051	T10489	T10488	prep	of,potential
R7052	T10490	T10491	det	each,origin
R7053	T10491	T10489	pobj	origin,of
R7054	T10492	T10491	compound	replication,origin
R7055	T10493	T10491	prep	on,origin
R7056	T10494	T10495	det	the,genome
R7057	T10495	T10493	pobj	genome,on
R7058	T10496	T10497	advmod	even,within
R7059	T10497	T10485	prep	within,variability
R7060	T10498	T10499	det	the,type
R7061	T10499	T10497	pobj	type,within
R7062	T10500	T10499	amod	single,type
R7063	T10501	T10499	compound	cell,type
R7064	T10502	T10456	punct	.,be
R7067	T10855	T10856	nmod	Mcm4,helicase
R7068	T10857	T10855	punct	/,Mcm4
R7069	T10858	T10855	nummod	6,Mcm4
R7070	T10859	T10855	punct	/,Mcm4
R7071	T10860	T10855	nummod	7,Mcm4
R7072	T10861	T10856	prep	during,helicase
R7073	T10862	T10863	amod	processive,unwinding
R7074	T10863	T10861	pobj	unwinding,during
R7075	T10864	T10856	prep	at,helicase
R7076	T10865	T10866	det	the,fork
R7077	T10866	T10864	pobj	fork,at
R7078	T10868	T10869	det	The,requirement
R7079	T10869	T10871	nsubj	requirement,prompted
R7080	T10870	T10869	amod	specific,requirement
R7081	T10872	T10869	prep	of,requirement
R7082	T10873	T10874	amod	single,stranded
R7083	T10874	T10876	amod	stranded,residues
R7084	T10875	T10874	punct	-,stranded
R7085	T10876	T10872	pobj	residues,of
R7086	T10877	T10876	compound	thymine,residues
R7087	T10878	T10869	prep	for,requirement
R7088	T10879	T10878	pobj	activation,for
R7089	T10880	T10879	prep	of,activation
R7090	T10881	T10882	compound	Mcm,helicase
R7091	T10882	T10880	pobj	helicase,of
R7092	T10883	T10871	dobj	us,prompted
R7093	T10884	T10885	aux	to,examine
R7094	T10885	T10871	xcomp	examine,prompted
R7095	T10886	T10887	mark	whether,required
R7096	T10887	T10885	ccomp	required,examine
R7097	T10888	T10887	nsubjpass	they,required
R7098	T10889	T10887	auxpass	are,required
R7099	T10890	T10887	advmod	also,required
R7100	T10891	T10887	prep	for,required
R7101	T10892	T10893	amod	processive,unwinding
R7102	T10893	T10891	pobj	unwinding,for
R7103	T10894	T10893	prep	of,unwinding
R7104	T10895	T10896	compound	duplex,DNA
R7105	T10896	T10894	pobj	DNA,of
R7106	T10897	T10871	punct	.,prompted
R7107	T10899	T10900	poss	Our,results
R7108	T10900	T10901	nsubj	results,indicated
R7109	T10902	T10903	mark	that,inhibited
R7110	T10903	T10901	ccomp	inhibited,indicated
R7111	T10904	T10903	nsubj	increase,inhibited
R7112	T10905	T10904	prep	of,increase
R7113	T10906	T10907	compound	GC,pair
R7114	T10907	T10905	pobj	pair,of
R7115	T10908	T10904	prep	in,increase
R7116	T10909	T10910	det	the,segment
R7117	T10910	T10908	pobj	segment,in
R7118	T10911	T10910	compound	duplex,segment
R7119	T10912	T10903	advmod	significantly,inhibited
R7120	T10913	T10914	det	the,activity
R7121	T10914	T10903	dobj	activity,inhibited
R7122	T10915	T10916	compound	Mcm,helicase
R7123	T10916	T10914	compound	helicase,activity
R7124	T10917	T10901	punct	.,indicated
R7125	T10919	T10920	compound	Duplex,DNA
R7126	T10920	T10921	nsubjpass	DNA,displaced
R7127	T10922	T10920	acl	composed,DNA
R7128	T10923	T10924	advmod	only,of
R7129	T10924	T10922	prep	of,composed
R7130	T10925	T10926	compound	GC,pairs
R7131	T10926	T10924	pobj	pairs,of
R7132	T10927	T10928	punct	(,repeats
R7133	T10928	T10926	parataxis	repeats,pairs
R7134	T10929	T10928	nummod	10,repeats
R7135	T10930	T10928	prep	of,repeats
R7136	T10931	T10930	pobj	CGG,of
R7137	T10932	T10928	punct	),repeats
R7138	T10933	T10926	prep	on,pairs
R7139	T10934	T10935	det	a,fork
R7140	T10935	T10933	pobj	fork,on
R7141	T10936	T10935	compound	Y,fork
R7142	T10937	T10935	punct	-,fork
R7143	T10938	T10921	auxpass	was,displaced
R7144	T10939	T10921	neg	not,displaced
R7145	T10940	T10941	advmod	at,all
R7146	T10941	T10921	advmod	all,displaced
R7147	T10942	T10921	punct	", ",displaced
R7148	T10943	T10944	mark	while,displaced
R7149	T10944	T10921	advcl	displaced,displaced
R7150	T10945	T10946	det	the,template
R7151	T10946	T10944	nsubjpass	template,displaced
R7152	T10947	T10946	amod	same,template
R7153	T10948	T10944	auxpass	was,displaced
R7154	T10949	T10944	advmod	readily,displaced
R7155	T10950	T10944	agent	by,displaced
R7156	T10951	T10952	compound	SV40,antigen
R7157	T10952	T10950	pobj	antigen,by
R7158	T10953	T10952	compound	T,antigen
R7159	T10954	T10952	punct	-,antigen
R7160	T10955	T10956	punct	(,8B
R7161	T10956	T10944	parataxis	8B,displaced
R7162	T10957	T10956	compound	Figure,8B
R7163	T10958	T10956	punct	),8B
R7164	T10959	T10921	punct	.,displaced
R7165	T10961	T10962	compound	Mcm,helicase
R7166	T10962	T10963	nsubjpass	helicase,inhibited
R7167	T10964	T10963	auxpass	was,inhibited
R7168	T10965	T10963	agent	by,inhibited
R7169	T10966	T10967	det	the,presence
R7170	T10967	T10965	pobj	presence,by
R7171	T10968	T10967	prep	of,presence
R7172	T10969	T10970	npadvmod	GC,rich
R7173	T10970	T10972	amod	rich,segment
R7174	T10971	T10970	punct	-,rich
R7175	T10972	T10968	pobj	segment,of
R7176	T10973	T10972	compound	duplex,segment
R7177	T10974	T10975	advmod	also,on
R7178	T10975	T10967	prep	on,presence
R7179	T10976	T10977	det	a,substrate
R7180	T10977	T10975	pobj	substrate,on
R7181	T10978	T10977	amod	circular,substrate
R7182	T10979	T10980	amod	single,stranded
R7183	T10980	T10977	amod	stranded,substrate
R7184	T10981	T10980	punct	-,stranded
R7185	T10982	T10983	amod	partial,heteroduplex
R7186	T10983	T10977	compound	heteroduplex,substrate
R7187	T10984	T10963	punct	.,inhibited
R7188	T10986	T10987	advmod	However,was
R7189	T10988	T10987	punct	", ",was
R7190	T10989	T10987	prep	on,was
R7191	T10990	T10991	det	this,substrate
R7192	T10991	T10989	pobj	substrate,on
R7193	T10992	T10987	punct	", ",was
R7194	T10993	T10987	nsubj	Mcm4,was
R7195	T10994	T10993	punct	/,Mcm4
R7196	T10995	T10993	nummod	6,Mcm4
R7197	T10996	T10993	punct	/,Mcm4
R7198	T10997	T10993	nummod	7,Mcm4
R7199	T10998	T10987	acomp	able,was
R7200	T10999	T11000	aux	to,displace
R7201	T11000	T10998	xcomp	displace,able
R7202	T11001	T11000	dobj	DNA,displace
R7203	T11002	T11000	prep	past,displace
R7204	T11003	T11004	det	the,region
R7205	T11004	T11002	pobj	region,past
R7206	T11005	T11006	npadvmod	GC,rich
R7207	T11006	T11004	amod	rich,region
R7208	T11007	T11006	punct	-,rich
R7209	T11008	T11000	punct	", ",displace
R7210	T11009	T11010	dep	albeit,to
R7211	T11010	T11000	prep	to,displace
R7212	T11011	T11012	det	a,extent
R7213	T11012	T11010	pobj	extent,to
R7214	T11013	T11012	amod	limited,extent
R7215	T11014	T11000	punct	", ",displace
R7216	T11015	T11016	advmod	when,added
R7217	T11016	T11000	advcl	added,displace
R7218	T11017	T11016	nsubjpass	it,added
R7219	T11018	T11016	auxpass	was,added
R7220	T11019	T11016	prep	at,added
R7221	T11020	T11021	det	a,concentration
R7222	T11021	T11019	pobj	concentration,at
R7223	T11022	T11021	amod	high,concentration
R7224	T11023	T10987	punct	.,was
R7225	T11025	T11026	prep	On,loaded
R7226	T11027	T11028	det	the,template
R7227	T11028	T11025	pobj	template,On
R7228	T11029	T11030	amod	partial,heteroduplex
R7229	T11030	T11028	compound	heteroduplex,template
R7230	T11031	T11026	punct	", ",loaded
R7231	T11032	T11026	nsubjpass	Mcm,loaded
R7232	T11033	T11026	auxpass	is,loaded
R7233	T11034	T11026	prep	onto,loaded
R7234	T11035	T11036	det	the,DNA
R7235	T11036	T11034	pobj	DNA,onto
R7236	T11037	T11036	amod	circular,DNA
R7237	T11038	T11039	amod	single,stranded
R7238	T11039	T11036	amod	stranded,DNA
R7239	T11040	T11039	punct	-,stranded
R7240	T11041	T11036	prep	of,DNA
R7241	T11042	T11043	nummod	6.4,kb
R7242	T11043	T11041	pobj	kb,of
R7243	T11044	T11026	punct	", ",loaded
R7244	T11045	T11046	mark	while,loaded
R7245	T11046	T11026	advcl	loaded,loaded
R7246	T11047	T11046	nsubjpass	it,loaded
R7247	T11048	T11046	auxpass	is,loaded
R7248	T11049	T11046	prep	onto,loaded
R7249	T11050	T11051	det	the,DNA
R7250	T11051	T11049	pobj	DNA,onto
R7251	T11052	T11053	nummod	50,nt
R7252	T11053	T11054	npadvmod	nt,long
R7253	T11054	T11051	amod	long,DNA
R7254	T11055	T11056	nummod	3,tail
R7255	T11056	T11051	compound	tail,DNA
R7256	T11057	T11055	punct	′,3
R7257	T11058	T11056	punct	-,tail
R7258	T11059	T11051	prep	on,DNA
R7259	T11060	T11061	det	the,fork
R7260	T11061	T11059	pobj	fork,on
R7261	T11062	T11061	compound	Y,fork
R7262	T11063	T11061	punct	-,fork
R7263	T11064	T11026	punct	.,loaded
R7264	T11066	T11067	advmod	Thus,reflect
R7265	T11068	T11067	punct	", ",reflect
R7266	T11069	T11070	det	the,difference
R7267	T11070	T11067	nsubj	difference,reflect
R7268	T11071	T11070	prep	of,difference
R7269	T11072	T11073	compound	helicase,actions
R7270	T11073	T11071	pobj	actions,of
R7271	T11074	T11067	aux	may,reflect
R7272	T11075	T11076	det	the,efficiency
R7273	T11076	T11067	dobj	efficiency,reflect
R7274	T11077	T11076	prep	of,efficiency
R7275	T11078	T11079	compound	Mcm,loading
R7276	T11079	T11077	pobj	loading,of
R7277	T11080	T11067	punct	.,reflect
R7278	T11082	T11083	advmod	Alternatively,engage
R7279	T11084	T11083	punct	", ",engage
R7280	T11085	T11086	det	the,presence
R7281	T11086	T11083	nsubj	presence,engage
R7282	T11087	T11086	prep	of,presence
R7283	T11088	T11087	punct	‘,of
R7284	T11089	T11090	amod	random,sequence
R7285	T11090	T11087	pobj	sequence,of
R7286	T11091	T11090	punct	’,sequence
R7287	T11092	T11086	prep	at,presence
R7288	T11093	T11094	det	the,segment
R7289	T11094	T11092	pobj	segment,at
R7290	T11095	T11096	advmod	initially,unwound
R7291	T11096	T11094	amod	unwound,segment
R7292	T11097	T11094	compound	duplex,segment
R7293	T11098	T11094	prep	in,segment
R7294	T11099	T11100	det	the,template
R7295	T11100	T11098	pobj	template,in
R7296	T11101	T11100	amod	former,template
R7297	T11102	T11083	aux	may,engage
R7298	T11103	T11104	det	the,helicase
R7299	T11104	T11083	dobj	helicase,engage
R7300	T11105	T11104	compound	Mcm,helicase
R7301	T11106	T11083	prep	in,engage
R7302	T11107	T11108	det	a,conformation
R7303	T11108	T11106	pobj	conformation,in
R7304	T11109	T11110	advmod	more,active
R7305	T11110	T11108	amod	active,conformation
R7306	T11111	T11112	dep	which,displace
R7307	T11112	T11108	relcl	displace,conformation
R7308	T11113	T11112	aux	can,displace
R7309	T11114	T11115	det	the,segment
R7310	T11115	T11112	dobj	segment,displace
R7311	T11116	T11117	npadvmod	GC,rich
R7312	T11117	T11115	amod	rich,segment
R7313	T11118	T11117	punct	-,rich
R7314	T11119	T11115	compound	duplex,segment
R7315	T11120	T11083	punct	.,engage
R7316	T11122	T11123	prep	On,restored
R7317	T11124	T11125	compound	Y,fork
R7318	T11125	T11127	compound	fork,substrates
R7319	T11126	T11125	punct	-,fork
R7320	T11127	T11122	pobj	substrates,On
R7321	T11128	T11123	punct	", ",restored
R7322	T11129	T11123	nsubj	increase,restored
R7323	T11130	T11129	prep	of,increase
R7324	T11131	T11132	compound	AT,pairs
R7325	T11132	T11130	pobj	pairs,of
R7326	T11133	T11132	compound	base,pairs
R7327	T11134	T11129	prep	in,increase
R7328	T11135	T11136	det	the,duplex
R7329	T11136	T11134	pobj	duplex,in
R7330	T11137	T11138	punct	(,repeats
R7331	T11138	T11136	parataxis	repeats,duplex
R7332	T11139	T11138	nummod	10,repeats
R7333	T11140	T11138	prep	of,repeats
R7334	T11141	T11140	pobj	CTT,of
R7335	T11142	T11138	punct	),repeats
R7336	T11143	T11144	det	the,unwinding
R7337	T11144	T11123	dobj	unwinding,restored
R7338	T11145	T11123	punct	.,restored
R7339	T11147	T11148	det	These,results
R7340	T11148	T11149	nsubj	results,indicate
R7341	T11150	T11151	preconj	either,required
R7342	T11151	T11149	advcl	required,indicate
R7343	T11152	T11151	mark	that,required
R7344	T11153	T11154	compound	thymine,residues
R7345	T11154	T11151	nsubjpass	residues,required
R7346	T11155	T11151	auxpass	are,required
R7347	T11156	T11151	prep	on,required
R7348	T11157	T11158	det	the,DNA
R7349	T11158	T11156	pobj	DNA,on
R7350	T11159	T11158	compound	duplex,DNA
R7351	T11160	T11151	prep	for,required
R7352	T11161	T11162	amod	continuous,unwinding
R7353	T11162	T11160	pobj	unwinding,for
R7354	T11163	T11151	punct	", ",required
R7355	T11164	T11151	cc	or,required
R7356	T11165	T11166	mark	that,is
R7357	T11166	T11151	conj	is,required
R7358	T11167	T11166	nsubj	Mcm4,is
R7359	T11168	T11167	punct	/,Mcm4
R7360	T11169	T11167	nummod	6,Mcm4
R7361	T11170	T11167	punct	/,Mcm4
R7362	T11171	T11167	nummod	7,Mcm4
R7363	T11172	T11166	advmod	simply,is
R7364	T11173	T11166	neg	not,is
R7365	T11174	T11166	acomp	efficient,is
R7366	T11175	T11174	advmod	enough,efficient
R7367	T11176	T11177	aux	to,unwind
R7368	T11177	T11174	xcomp	unwind,efficient
R7369	T11178	T11179	det	the,pairs
R7370	T11179	T11177	dobj	pairs,unwind
R7371	T11180	T11179	amod	stable,pairs
R7372	T11181	T11179	compound	GC,pairs
R7373	T11182	T11149	punct	.,indicate
R7374	T11184	T11185	nsubj	Replacement,resulted
R7375	T11186	T11184	prep	of,Replacement
R7376	T11187	T11188	det	the,guanosine
R7377	T11188	T11186	pobj	guanosine,of
R7378	T11189	T11188	amod	central,guanosine
R7379	T11190	T11184	prep	with,Replacement
R7380	T11191	T11190	pobj	inosine,with
R7381	T11192	T11184	prep	in,Replacement
R7382	T11193	T11194	det	the,DNA
R7383	T11194	T11192	pobj	DNA,in
R7384	T11195	T11196	compound	CGG,repeat
R7385	T11196	T11194	compound	repeat,DNA
R7386	T11197	T11194	compound	duplex,DNA
R7387	T11198	T11185	prep	in,resulted
R7388	T11199	T11198	pobj	displacement,in
R7389	T11200	T11201	punct	(,8E
R7390	T11201	T11185	parataxis	8E,resulted
R7391	T11202	T11201	compound	Figure,8E
R7392	T11203	T11201	punct	),8E
R7393	T11204	T11205	punct	(,31
R7394	T11205	T11185	parataxis	31,resulted
R7395	T11206	T11205	punct	),31
R7396	T11207	T11185	punct	", ",resulted
R7397	T11208	T11185	advcl	suggesting,resulted
R7398	T11209	T11210	mark	that,be
R7399	T11210	T11208	advcl	be,suggesting
R7400	T11211	T11212	det	the,presence
R7401	T11212	T11210	nsubj	presence,be
R7402	T11213	T11212	amod	continuous,presence
R7403	T11214	T11212	prep	of,presence
R7404	T11215	T11216	compound	AT,pair
R7405	T11216	T11214	pobj	pair,of
R7406	T11217	T11216	compound	base,pair
R7407	T11218	T11210	aux	may,be
R7408	T11219	T11210	neg	not,be
R7409	T11220	T11210	acomp	essential,be
R7410	T11221	T11220	prep	for,essential
R7411	T11222	T11223	det	the,function
R7412	T11223	T11221	pobj	function,for
R7413	T11224	T11225	compound	Mcm,helicase
R7414	T11225	T11223	compound	helicase,function
R7415	T11226	T11210	cc	and,be
R7416	T11227	T11228	mark	that,is
R7417	T11228	T11210	conj	is,be
R7418	T11229	T11230	det	the,thermostability
R7419	T11230	T11228	nsubj	thermostability,is
R7420	T11231	T11230	prep	of,thermostability
R7421	T11232	T11233	compound	GC,pairs
R7422	T11233	T11231	pobj	pairs,of
R7423	T11234	T11233	compound	base,pairs
R7424	T11235	T11228	acomp	inhibitory,is
R7425	T11236	T11235	prep	for,inhibitory
R7426	T11237	T11238	compound	helicase,action
R7427	T11238	T11236	pobj	action,for
R7428	T11239	T11238	prep	of,action
R7429	T11240	T11239	pobj	Mcm4,of
R7430	T11241	T11240	punct	/,Mcm4
R7431	T11242	T11240	nummod	6,Mcm4
R7432	T11243	T11240	punct	/,Mcm4
R7433	T11244	T11240	nummod	7,Mcm4
R7434	T11245	T11185	punct	.,resulted
R7435	T11247	T11248	det	The,results
R7436	T11248	T11249	nsubj	results,reveal
R7437	T11250	T11248	acl	described,results
R7438	T11251	T11250	prep	in,described
R7439	T11252	T11253	det	this,manuscript
R7440	T11253	T11251	pobj	manuscript,in
R7441	T11254	T11255	advmod	potentially,important
R7442	T11255	T11256	amod	important,features
R7443	T11256	T11249	dobj	features,reveal
R7444	T11257	T11256	prep	of,features
R7445	T11258	T11259	amod	mammalian,helicase
R7446	T11259	T11257	pobj	helicase,of
R7447	T11260	T11259	nmod	Mcm4,helicase
R7448	T11261	T11260	punct	/,Mcm4
R7449	T11262	T11260	nummod	6,Mcm4
R7450	T11263	T11260	punct	/,Mcm4
R7451	T11264	T11260	nummod	7,Mcm4
R7452	T11265	T11259	punct	", ",helicase
R7453	T11266	T11267	dep	which,is
R7454	T11267	T11259	relcl	is,helicase
R7455	T11268	T11267	acomp	likely,is
R7456	T11269	T11270	aux	to,be
R7457	T11270	T11268	xcomp	be,likely
R7458	T11271	T11272	det	a,component
R7459	T11272	T11270	attr	component,be
R7460	T11273	T11272	amod	key,component
R7461	T11274	T11272	prep	of,component
R7462	T11275	T11276	det	the,helicase
R7463	T11276	T11274	pobj	helicase,of
R7464	T11277	T11276	amod	eukaryotic,helicase
R7465	T11278	T11276	amod	replicative,helicase
R7466	T11279	T11249	punct	.,reveal
R7467	T11281	T11282	amod	Prior,to
R7468	T11282	T11283	prep	to,adopt
R7469	T11284	T11282	pobj	initiation,to
R7470	T11285	T11284	prep	of,initiation
R7471	T11286	T11287	compound	DNA,replication
R7472	T11287	T11285	pobj	replication,of
R7473	T11288	T11283	punct	", ",adopt
R7474	T11289	T11290	compound	Mcm,helicase
R7475	T11290	T11283	nsubj	helicase,adopt
R7476	T11291	T11283	aux	may,adopt
R7477	T11292	T11293	det	a,complex
R7478	T11293	T11283	dobj	complex,adopt
R7479	T11294	T11295	amod	double,hexameric
R7480	T11295	T11293	amod	hexameric,complex
R7481	T11296	T11283	prep	at,adopt
R7482	T11297	T11298	det	the,region
R7483	T11298	T11296	pobj	region,at
R7484	T11299	T11300	advmod	partially,melted
R7485	T11300	T11298	amod	melted,region
R7486	T11301	T11298	compound	origin,region
R7487	T11302	T11283	punct	", ",adopt
R7488	T11303	T11283	cc	and,adopt
R7489	T11304	T11305	aux	may,catalyze
R7490	T11305	T11283	conj	catalyze,adopt
R7491	T11306	T11307	amod	concurrent,unwinding
R7492	T11307	T11305	dobj	unwinding,catalyze
R7493	T11308	T11307	prep	of,unwinding
R7494	T11309	T11310	compound	duplex,DNA
R7495	T11310	T11308	pobj	DNA,of
R7496	T11311	T11305	prep	into,catalyze
R7497	T11312	T11313	det	both,directions
R7498	T11313	T11311	pobj	directions,into
R7499	T11314	T11283	punct	", ",adopt
R7500	T11315	T11316	mark	while,associated
R7501	T11316	T11283	advcl	associated,adopt
R7502	T11317	T11316	advmod	stably,associated
R7503	T11318	T11316	prep	with,associated
R7504	T11319	T11320	det	the,origins
R7505	T11320	T11318	pobj	origins,with
R7506	T11321	T11320	prep	of,origins
R7507	T11322	T11323	compound	DNA,replication
R7508	T11323	T11321	pobj	replication,of
R7509	T11324	T11283	punct	.,adopt
R7510	T11326	T11327	nsubj	This,is
R7511	T11328	T11327	acomp	similar,is
R7512	T11329	T11328	prep	to,similar
R7513	T11330	T11331	det	the,model
R7514	T11331	T11329	pobj	model,to
R7515	T11332	T11333	compound	T,antigen
R7516	T11333	T11331	compound	antigen,model
R7517	T11334	T11333	punct	-,antigen
R7518	T11335	T11336	advmod	originally,proposed
R7519	T11336	T11331	acl	proposed,model
R7520	T11337	T11336	agent	by,proposed
R7521	T11338	T11337	pobj	Smelkova,by
R7522	T11339	T11338	cc	and,Smelkova
R7523	T11340	T11338	conj	Borowiec,Smelkova
R7524	T11341	T11342	punct	(,21
R7525	T11342	T11340	parataxis	21,Borowiec
R7526	T11343	T11342	punct	),21
R7527	T11344	T11327	punct	.,is
R7528	T11346	T11347	nsubj	We,propose
R7529	T11348	T11349	mark	that,mobilized
R7530	T11349	T11347	ccomp	mobilized,propose
R7531	T11350	T11349	punct	", ",mobilized
R7532	T11351	T11352	advmod	only,activated
R7533	T11352	T11349	advcl	activated,mobilized
R7534	T11353	T11352	advmod	when,activated
R7535	T11354	T11355	det	both,hexamers
R7536	T11355	T11352	nsubjpass	hexamers,activated
R7537	T11356	T11352	auxpass	are,activated
R7538	T11357	T11352	agent	by,activated
R7539	T11358	T11359	det	the,sequences
R7540	T11359	T11357	pobj	sequences,by
R7541	T11360	T11359	amod	interacting,sequences
R7542	T11361	T11362	npadvmod	thymine,rich
R7543	T11362	T11359	amod	rich,sequences
R7544	T11363	T11362	punct	-,rich
R7545	T11364	T11359	relcl	present,sequences
R7546	T11365	T11364	prep	within,present
R7547	T11366	T11367	det	the,region
R7548	T11367	T11365	pobj	region,within
R7549	T11368	T11367	amod	melted,region
R7550	T11369	T11349	punct	", ",mobilized
R7551	T11370	T11371	det	the,helicase
R7552	T11371	T11349	nsubjpass	helicase,mobilized
R7553	T11372	T11371	compound	Mcm,helicase
R7554	T11373	T11349	auxpass	is,mobilized
R7555	T11374	T11349	cc	and,mobilized
R7556	T11375	T11376	nsubj	initiation,takes
R7557	T11376	T11349	conj	takes,mobilized
R7558	T11377	T11376	dobj	place,takes
R7559	T11378	T11347	punct	.,propose
R7560	T11380	T11381	det	A,question
R7561	T11381	T11383	nsubj	question,is
R7562	T11382	T11381	amod	crucial,question
R7563	T11384	T11385	advmod	how,generated
R7564	T11385	T11383	ccomp	generated,is
R7565	T11386	T11387	det	these,complexes
R7566	T11387	T11385	nsubjpass	complexes,generated
R7567	T11388	T11389	advmod	double,hexameric
R7568	T11389	T11387	amod	hexameric,complexes
R7569	T11390	T11387	compound	helicase,complexes
R7570	T11391	T11385	auxpass	are,generated
R7571	T11392	T11385	cc	and,generated
R7572	T11393	T11385	conj	turn,generated
R7573	T11394	T11393	prep	into,turn
R7574	T11395	T11396	amod	active,helicases
R7575	T11396	T11394	pobj	helicases,into
R7576	T11397	T11393	prep	at,turn
R7577	T11398	T11399	det	the,origins
R7578	T11399	T11397	pobj	origins,at
R7579	T11400	T11383	punct	.,is
R7580	T11402	T11403	det	This,process
R7581	T11403	T11404	nsubj	process,involve
R7582	T11405	T11404	aux	would,involve
R7583	T11406	T11404	dobj	melting,involve
R7584	T11407	T11406	prep	of,melting
R7585	T11408	T11409	compound	duplex,DNA
R7586	T11409	T11407	pobj	DNA,of
R7587	T11410	T11406	punct	", ",melting
R7588	T11411	T11412	dep	which,facilitated
R7589	T11412	T11406	relcl	facilitated,melting
R7590	T11413	T11412	aux	may,facilitated
R7591	T11414	T11412	auxpass	be,facilitated
R7592	T11415	T11412	prep	by,facilitated
R7593	T11416	T11415	pobj	binding,by
R7594	T11417	T11416	prep	of,binding
R7595	T11418	T11419	compound	preRC,components
R7596	T11419	T11417	pobj	components,of
R7597	T11420	T11416	prep	in,binding
R7598	T11421	T11422	det	the,context
R7599	T11422	T11420	pobj	context,in
R7600	T11423	T11422	prep	of,context
R7601	T11424	T11425	amod	proper,structures
R7602	T11425	T11423	pobj	structures,of
R7603	T11426	T11425	compound	chromatin,structures
R7604	T11427	T11415	punct	", ",by
R7605	T11428	T11415	cc	or,by
R7606	T11429	T11415	conj	by,by
R7607	T11430	T11431	amod	other,factors
R7608	T11431	T11429	pobj	factors,by
R7609	T11432	T11431	amod	unwinding,factors
R7610	T11433	T11431	prep	including,factors
R7611	T11434	T11435	det	a,topoisomerase
R7612	T11435	T11433	pobj	topoisomerase,including
R7613	T11436	T11435	cc	or,topoisomerase
R7614	T11437	T11438	det	a,helicase
R7615	T11438	T11435	conj	helicase,topoisomerase
R7616	T11439	T11440	advmod	newly,identified
R7617	T11440	T11438	amod	identified,helicase
R7618	T11441	T11438	compound	Mcm8,helicase
R7619	T11442	T11443	punct	(,34
R7620	T11443	T11404	parataxis	34,involve
R7621	T11444	T11445	punct	–,36
R7622	T11445	T11443	prep	36,34
R7623	T11446	T11443	punct	),34
R7624	T11447	T11404	punct	.,involve
R7625	T11449	T11450	nsubj	It,require
R7626	T11451	T11450	aux	may,require
R7627	T11452	T11450	advmod	also,require
R7628	T11453	T11450	dobj	association,require
R7629	T11454	T11453	prep	of,association
R7630	T11455	T11456	npadvmod	fork,assisting
R7631	T11456	T11458	amod	assisting,proteins
R7632	T11457	T11456	punct	-,assisting
R7633	T11458	T11454	pobj	proteins,of
R7634	T11459	T11460	amod	such,as
R7635	T11460	T11458	prep	as,proteins
R7636	T11461	T11460	pobj	Cdc45,as
R7637	T11462	T11463	punct	(,38
R7638	T11463	T11461	parataxis	38,Cdc45
R7639	T11464	T11463	nummod	37,38
R7640	T11465	T11463	punct	",",38
R7641	T11466	T11463	punct	),38
R7642	T11467	T11468	advmod	as,as
R7643	T11468	T11458	cc	as,proteins
R7644	T11469	T11468	advmod	well,as
R7645	T11470	T11471	compound	phosphorylation,events
R7646	T11471	T11458	conj	events,proteins
R7647	T11472	T11471	prep	by,events
R7648	T11473	T11474	nmod	Cdk,kinases
R7649	T11474	T11472	pobj	kinases,by
R7650	T11475	T11473	cc	and,Cdk
R7651	T11476	T11473	conj	Cdc7,Cdk
R7652	T11477	T11478	punct	(,40
R7653	T11478	T11474	parataxis	40,kinases
R7654	T11479	T11478	nummod	39,40
R7655	T11480	T11478	punct	",",40
R7656	T11481	T11478	punct	),40
R7657	T11482	T11450	punct	.,require
R7658	T11484	T11485	nummod	One,finding
R7659	T11485	T11487	nsubj	finding,is
R7660	T11486	T11485	amod	unexpected,finding
R7661	T11488	T11485	prep	of,finding
R7662	T11489	T11490	det	this,study
R7663	T11490	T11488	pobj	study,of
R7664	T11491	T11492	amod	low,activity
R7665	T11492	T11487	attr	activity,is
R7666	T11493	T11492	compound	helicase,activity
R7667	T11494	T11492	prep	of,activity
R7668	T11495	T11494	pobj	Mcm4,of
R7669	T11496	T11495	punct	/,Mcm4
R7670	T11497	T11495	nummod	6,Mcm4
R7671	T11498	T11495	punct	/,Mcm4
R7672	T11499	T11495	nummod	7,Mcm4
R7673	T11500	T11492	prep	on,activity
R7674	T11501	T11502	npadvmod	GC,rich
R7675	T11502	T11504	amod	rich,DNA
R7676	T11503	T11502	punct	-,rich
R7677	T11504	T11500	pobj	DNA,on
R7678	T11505	T11504	compound	duplex,DNA
R7679	T11506	T11487	punct	.,is
R7680	T11508	T11509	nsubj	This,indicates
R7681	T11510	T11508	punct	", ",This
R7682	T11511	T11508	prep	in,This
R7683	T11512	T11511	pobj	conjunction,in
R7684	T11513	T11512	prep	with,conjunction
R7685	T11514	T11515	det	the,processivity
R7686	T11515	T11513	pobj	processivity,with
R7687	T11516	T11515	amod	low,processivity
R7688	T11517	T11515	prep	of,processivity
R7689	T11518	T11519	nmod	Mcm4,helicase
R7690	T11519	T11517	pobj	helicase,of
R7691	T11520	T11518	punct	/,Mcm4
R7692	T11521	T11518	nummod	6,Mcm4
R7693	T11522	T11518	punct	/,Mcm4
R7694	T11523	T11518	nummod	7,Mcm4
R7695	T11524	T11509	punct	", ",indicates
R7696	T11525	T11509	advmod	strongly,indicates
R7697	T11526	T11527	mark	that,require
R7698	T11527	T11509	ccomp	require,indicates
R7699	T11528	T11529	det	the,helicase
R7700	T11529	T11527	nsubj	helicase,require
R7701	T11530	T11529	amod	replicative,helicase
R7702	T11531	T11529	prep	at,helicase
R7703	T11532	T11533	det	the,forks
R7704	T11533	T11531	pobj	forks,at
R7705	T11534	T11533	compound	replication,forks
R7706	T11535	T11527	aux	would,require
R7707	T11536	T11527	dobj	more,require
R7708	T11537	T11536	prep	than,more
R7709	T11538	T11539	nmod	Mcm4,complex
R7710	T11539	T11537	pobj	complex,than
R7711	T11540	T11538	punct	/,Mcm4
R7712	T11541	T11538	nummod	6,Mcm4
R7713	T11542	T11538	punct	/,Mcm4
R7714	T11543	T11538	nummod	7,Mcm4
R7715	T11544	T11509	punct	.,indicates
R7716	T11546	T11547	prep	During,stimulated
R7717	T11548	T11549	det	the,unwinding
R7718	T11549	T11546	pobj	unwinding,During
R7719	T11550	T11549	amod	processive,unwinding
R7720	T11551	T11549	prep	of,unwinding
R7721	T11552	T11553	compound	duplex,forks
R7722	T11553	T11551	pobj	forks,of
R7723	T11554	T11553	compound	DNA,forks
R7724	T11555	T11553	compound	replication,forks
R7725	T11556	T11547	punct	", ",stimulated
R7726	T11557	T11547	nsubjpass	Mcm4,stimulated
R7727	T11558	T11557	punct	/,Mcm4
R7728	T11559	T11557	nummod	6,Mcm4
R7729	T11560	T11557	punct	/,Mcm4
R7730	T11561	T11557	nummod	7,Mcm4
R7731	T11562	T11547	aux	may,stimulated
R7732	T11563	T11547	auxpass	be,stimulated
R7733	T11564	T11547	advmod	further,stimulated
R7734	T11565	T11547	agent	by,stimulated
R7735	T11566	T11565	pobj	interaction,by
R7736	T11567	T11566	prep	with,interaction
R7737	T11568	T11569	amod	other,proteins
R7738	T11569	T11567	pobj	proteins,with
R7739	T11570	T11569	punct	", ",proteins
R7740	T11571	T11569	prep	including,proteins
R7741	T11572	T11573	amod	remaining,subunits
R7742	T11573	T11571	pobj	subunits,including
R7743	T11574	T11573	compound	Mcm,subunits
R7744	T11575	T11573	punct	", ",subunits
R7745	T11576	T11573	conj	Cdc45,subunits
R7746	T11577	T11578	punct	(,38
R7747	T11578	T11576	parataxis	38,Cdc45
R7748	T11579	T11578	nummod	37,38
R7749	T11580	T11578	punct	",",38
R7750	T11581	T11578	punct	),38
R7751	T11582	T11576	punct	", ",Cdc45
R7752	T11583	T11576	conj	GINS,Cdc45
R7753	T11584	T11585	punct	(,42
R5896	T8790	T8791	compound	DNA,helicase
R5897	T8791	T8792	nsubj	helicase,is
R5898	T8793	T8794	det	a,factor
R999	T1672	T1670	pobj	complex,of
R1064	T1743	T1741	pobj	proteins,of
R1080	T1759	T1757	dobj	proteins,expressing
R3787	T5598	T5568	conj	unwound,unwound
R402	T888	T886	pobj	proteins,of
R416	T903	T910	nsubj	proteins,contain
R452	T942	T930	nsubjpass	complex,shown
R479	T970	T977	advcl	make,inhibit
R816	T1322	T1305	conj	related,binds
R7754	T11585	T11583	parataxis	42,GINS
R7755	T11586	T11585	nummod	41,42
R7756	T11587	T11585	punct	",",42
R7757	T11588	T11585	punct	),42
R7758	T11589	T11583	punct	", ",GINS
R7759	T11590	T11591	compound	DNA,subunits
R7760	T11591	T11583	conj	subunits,GINS
R7761	T11592	T11591	compound	polymerase,subunits
R7762	T11593	T11594	punct	(,43
R7763	T11594	T11591	parataxis	43,subunits
R7764	T11595	T11594	punct	),43
R7765	T11596	T11591	punct	", ",subunits
R7766	T11597	T11591	conj	Mcm8,subunits
R7767	T11598	T11599	punct	(,36
R7768	T11599	T11597	parataxis	36,Mcm8
R7769	T11600	T11599	nummod	35,36
R7770	T11601	T11599	punct	",",36
R7771	T11602	T11599	punct	),36
R7772	T11603	T11597	punct	", ",Mcm8
R7773	T11604	T11597	conj	etc.,Mcm8
R7774	T11605	T11606	aux	to,become
R7775	T11606	T11547	advcl	become,stimulated
R7776	T11607	T11608	det	a,helicase
R7777	T11608	T11606	attr	helicase,become
R7778	T11609	T11610	advmod	truly,processive
R7779	T11610	T11608	amod	processive,helicase
R7780	T11611	T11610	cc	and,processive
R7781	T11612	T11610	conj	potent,processive
R7782	T11613	T11608	amod	capable,helicase
R7783	T11614	T11613	prep	of,capable
R7784	T11615	T11614	pcomp	replicating,of
R7785	T11616	T11617	det	the,genome
R7786	T11617	T11615	dobj	genome,replicating
R7787	T11618	T11617	amod	entire,genome
R7788	T11619	T11547	punct	.,stimulated

craft-ca-core-ex-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id	Subject	Object	Predicate	Lexical cue
T83	0-3	CHEBI_SO_EXT:DNA	denotes	DNA
T84	4-11	CHEMINF_GO_EXT:chemical_binding_or_bond_formation	denotes	binding
T85	16-24	GO_EXT:0004386	denotes	helicase
T86	36-41	NCBITaxon:10088	denotes	mouse
T87	42-46	PR_EXT:000010246	denotes	MCM4
T88	42-50	GO:0097373	denotes	MCM4/6/7
T89	51-59	GO_EXT:0004386	denotes	helicase
T90	70-79	GO_EXT:0004386	denotes	Helicases
T91	102-112	_FRAGMENT	denotes	initiation
T92	128-146	GO:0006270	denotes	of DNA replication
T93	117-146	GO:0006271	denotes	elongation of DNA replication
T94	131-134	CHEBI_SO_EXT:DNA	denotes	DNA
T95	176-184	GO_EXT:0004386	denotes	helicase
T96	189-195	GO_EXT:0016887	denotes	ATPase
T97	214-223	NCBITaxon:40674	denotes	mammalian
T98	224-228	PR_EXT:000010246	denotes	Mcm4
T99	224-240	GO:0097373	denotes	Mcm4/6/7 complex
T100	271-278	CHEBI_EXT:thymine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	thymine
T101	284-303	CHEBI_SO_EXT:single_stranded_DNA	denotes	single-stranded DNA
T102	352-360	GO_EXT:0004386	denotes	helicase
T103	393-397	CHEBI_SO_EXT:DNA	denotes	DNAs
T104	422-426	PR_EXT:000010246	denotes	Mcm4
T105	422-430	GO:0097373	denotes	Mcm4/6/7
T106	485-487	CHEBI_SO_EXT:nucleotide	denotes	nt
T107	488-503	SO:0000984	denotes	single-stranded
T108	566-581	GO_EXT:double_hexameric_macromolecular_complex	denotes	double hexamers
T109	591-598	CHEBI_EXT:thymine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	thymine
T110	599-607	CHEBI_EXT:residue	denotes	residues
T111	749-765	GO_EXT:double_hexameric_macromolecular_complex	denotes	double hexameric
T112	766-770	PR_EXT:000010246	denotes	Mcm4
T113	766-774	GO:0097373	denotes	Mcm4/6/7
T114	829-833	PR_EXT:000010246	denotes	Mcm4
T115	829-837	GO:0097373	denotes	Mcm4/6/7
T116	838-843	CHEMINF_GO_EXT:chemical_binding_or_bond_formation	denotes	binds
T117	864-868	GO:0005657	denotes	fork
T118	905-920	_FRAGMENT	denotes	single-stranded
T119	929-932	CHEBI_SO_EXT:single_stranded_DNA	denotes	DNA
T120	948-956	GO_EXT:0004386	denotes	helicase
T121	983-991	SO_EXT:biological_sequence	denotes	sequence
T122	1053-1056	CHEBI_SO_EXT:DNA	denotes	DNA
T123	1074-1081	CHEBI_EXT:thymine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	thymine
T124	1091-1110	GO:0000732	denotes	Strand displacement
T125	1114-1118	PR_EXT:000010246	denotes	Mcm4
T126	1114-1122	GO:0097373	denotes	Mcm4/6/7
T127	1162-1168	SO:0000985	denotes	duplex
T128	1187-1198	SO_EXT:sequence_substitution_entity_or_process	denotes	Replacement
T129	1202-1210	CHEBI_EXT:cytosine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	cytosine
T130	1211-1218	CHEBI_EXT:guanine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	guanine
T131	1230-1238	CHEBI_EXT:cytosine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	cytosine
T132	1239-1246	CHEBI_SO_EXT:inosine	denotes	inosine
T133	1303-1312	NCBITaxon:40674	denotes	mammalian
T134	1313-1317	PR_EXT:000010246	denotes	Mcm4
T135	1313-1321	GO:0097373	denotes	Mcm4/6/7
T136	1322-1330	GO_EXT:0004386	denotes	helicase
T137	1368-1372	CHEBI_SO_EXT:base	denotes	base
T138	1368-1379	SO_EXT:0000028	denotes	base-paired
T139	1491-1501	NCBITaxon:2759	denotes	eukaryotic
T140	1502-1513	GO:0006260	denotes	replicative
T141	1514-1522	GO_EXT:0004386	denotes	helicase
T623	1538-1541	CHEBI_SO_EXT:DNA	denotes	DNA
T624	1538-1551	GO_EXT:0003678	denotes	DNA helicases
T625	1563-1573	MOP:0000619	denotes	hydrolysis
T626	1577-1587	_FRAGMENT	denotes	nucleotide
T627	1607-1619	CHEBI_EXT:nucleotide_triphosphate	denotes	triphosphate
T628	1591-1619	CHEBI_EXT:dNTP	denotes	deoxynucleotide triphosphate
T629	1623-1639	GO:0032508	denotes	duplex unwinding
T630	1694-1704	CHEBI_SO_EXT:nucleotide	denotes	nucleotide
T631	1705-1712	CHEMINF_GO_EXT:chemical_binding_or_bond_formation	denotes	binding
T632	1714-1717	CHEBI_SO_EXT:DNA	denotes	DNA
T633	1718-1725	CHEMINF_GO_EXT:chemical_binding_or_bond_formation	denotes	binding
T634	1730-1733	CHEBI_EXT:ATP	denotes	ATP
T635	1734-1744	MOP:0000619	denotes	hydrolysis
T636	1761-1784	GO:0032508	denotes	unwinding of duplex DNA
T637	1774-1784	CHEBI_SO_EXT:double_stranded_DNA	denotes	duplex DNA
T638	1796-1805	GO_EXT:hexameric_macromolecular_complex	denotes	hexameric
T639	1806-1815	GO_EXT:0004386	denotes	helicases
T640	1826-1852	GO:0042555	denotes	minichromosome maintenance
T641	1854-1857	GO:0042555	denotes	Mcm
T642	1859-1867	GO_EXT:0004386	denotes	helicase
T643	1944-1947	CHEBI_SO_EXT:DNA	denotes	DNA
T644	1944-1957	GO_EXT:0003678	denotes	DNA helicases
T645	1983-1998	NCBITaxon:10633	denotes	simian virus 40
T646	2007-2014	CHEBI_EXT:59132	denotes	antigen
T647	2020-2036	NCBITaxon:562	denotes	Escherichia coli
T648	2037-2041	PR_EXT:000022460	denotes	DnaB
T649	2050-2058	NCBITaxon:2157	denotes	Archaeal
T650	2117-2127	NCBITaxon:2759	denotes	eukaryotic
T651	2128-2131	GO:0042555	denotes	Mcm
T652	2132-2141	GO_EXT:0004386	denotes	helicases
T653	2162-2170	GO_EXT:trimeric_macromolecular_complex	denotes	trimeric
T654	2171-2175	PR_EXT:000010246	denotes	Mcm4
T655	2171-2179	GO:0097373	denotes	Mcm4/6/7
T656	2180-2188	CHEBI_PR_EXT:protein	denotes	proteins
T657	2210-2213	GO:0042555	denotes	Mcm
T658	2210-2215	PR_EXT:000010242	denotes	Mcm 2
T659	2210-2213	_FRAGMENT	denotes	Mcm
T660	2216-2217	PR_EXT:000010249	denotes	7
T661	2218-2226	CHEBI_PR_EXT:protein	denotes	proteins
T662	2242-2251	SO_EXT:biological_conservation_process_or_quality	denotes	conserved
T663	2252-2255	CHEBI_SO_EXT:DNA	denotes	DNA
T664	2252-2272	GO_EXT:0008094	denotes	DNA-dependent ATPase
T665	2273-2279	SO_EXT:sequence_or_structure_motif	denotes	motifs
T666	2342-2351	GO:0032991	denotes	complexes
T667	2358-2361	GO:0042555	denotes	MCM
T668	2362-2370	CHEBI_PR_EXT:protein	denotes	proteins
T669	2394-2398	PR_EXT:000010246	denotes	Mcm4
T670	2394-2410	GO:0097373	denotes	Mcm4/6/7 complex
T671	2450-2453	CHEBI_SO_EXT:DNA	denotes	DNA
T672	2450-2462	GO_EXT:0003678	denotes	DNA helicase
T673	2493-2497	PR_EXT:000010246	denotes	Mcm4
T674	2499-2503	PR_EXT:000010248	denotes	Mcm6
T675	2508-2512	PR_EXT:000010249	denotes	Mcm7
T676	2513-2521	CHEBI_PR_EXT:protein	denotes	proteins
T677	2556-2564	GO_EXT:0004386	denotes	helicase
T678	2583-2587	PR_EXT:000010242	denotes	Mcm2
T679	2591-2595	PR_EXT:000010243	denotes	Mcm3
T680	2610-2618	GO_EXT:0004386	denotes	helicase
T681	2635-2639	PR_EXT:000010246	denotes	Mcm4
T682	2635-2651	GO:0097373	denotes	Mcm4/6/7 complex
T683	2670-2683	GO_EXT:double_trimer_macromolecular_complex	denotes	double trimer
T684	2707-2715	GO_EXT:tetrameric_macromolecular_complex	denotes	tetramer
T685	2725-2733	GO_EXT:pentameric_macromolecular_complex	denotes	pentamer
T686	2756-2765	GO:0000785	denotes	Chromatin
T687	2797-2804	SO_EXT:0000704	denotes	genetic
T688	2825-2849	NCBITaxon:4932	denotes	Saccharomyces cerevisiae
T689	2874-2877	GO:0042555	denotes	Mcm
T690	2929-2932	CHEBI_SO_EXT:DNA	denotes	DNA
T691	2929-2949	GO:0022616	denotes	DNA chain elongation
T692	2961-2972	GO:0006260	denotes	replicative
T693	2973-2981	GO_EXT:0004386	denotes	helicase
T694	3044-3069	NCBITaxon:4896	denotes	Schizosaccharomyces pombe
T695	3074-3079	NCBITaxon:10088	denotes	mouse
T696	3080-3084	PR_EXT:000010246	denotes	Mcm4
T697	3080-3098	GO:0097373	denotes	Mcm4/6/7 complexes
T698	3130-3136	GO:0005657	denotes	forked
T699	3137-3140	CHEBI_SO_EXT:DNA	denotes	DNA
T700	3170-3180	CHEBI_SO_EXT:double_stranded_DNA	denotes	duplex DNA
T701	3192-3194	SO_EXT:0000028	denotes	bp
T702	3204-3208	PR_EXT:000010246	denotes	Mcm4
T703	3204-3212	GO:0097373	denotes	Mcm4/6/7
T704	3213-3218	CHEMINF_GO_EXT:chemical_binding_or_bond_formation	denotes	binds
T705	3222-3226	GO:0005657	denotes	fork
T706	3255-3258	CHEBI_EXT:ATP	denotes	ATP
T707	3293-3317	GO_EXT:double_hexameric_macromolecular_complex	denotes	double-hexameric complex
T708	3338-3345	CHEBI_EXT:59132	denotes	antigen
T709	3389-3397	GO_EXT:0004386	denotes	helicase
T710	3402-3405	CHEBI_EXT:ATP	denotes	ATP
T711	3406-3416	MOP:0000619	denotes	hydrolysis
T712	3431-3440	NCBITaxon:40674	denotes	mammalian
T713	3441-3445	PR_EXT:000010246	denotes	Mcm4
T714	3441-3449	GO:0097373	denotes	Mcm4/6/7
T715	3480-3499	CHEBI_SO_EXT:single_stranded_DNA	denotes	single-stranded DNA
T716	3524-3531	CHEBI_EXT:thymine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	thymine
T717	3532-3540	CHEBI_EXT:residue	denotes	residues
T718	3573-3576	GO:0042555	denotes	Mcm
T719	3617-3628	GO:0006260	denotes	replication
T720	3617-3636	SO_EXT:0000296	denotes	replication origins
T721	3647-3657	NCBITaxon:2759	denotes	eukaryotes
T722	3726-3729	GO:0042555	denotes	Mcm
T723	3730-3738	GO_EXT:0004386	denotes	helicase
T724	3790-3801	GO:0006260	denotes	replication
T725	3790-3807	GO:0005657	denotes	replication forks
T726	3856-3864	GO_EXT:0004386	denotes	helicase
T727	3876-3879	CHEBI_SO_EXT:DNA	denotes	DNA
T728	3880-3887	CHEMINF_GO_EXT:chemical_binding_or_bond_formation	denotes	binding
T729	3891-3896	NCBITaxon:10088	denotes	mouse
T730	3897-3901	PR_EXT:000010246	denotes	Mcm4
T731	3897-3905	GO:0097373	denotes	Mcm4/6/7
T732	3906-3914	GO_EXT:0004386	denotes	helicase
T733	3929-3935	GO:0005657	denotes	forked
T734	3957-3961	CHEBI_SO_EXT:DNA	denotes	DNAs
T735	3989-3997	SO_EXT:biological_sequence	denotes	sequence
T736	4018-4021	GO:0042555	denotes	Mcm
T737	4022-4030	GO_EXT:0004386	denotes	helicase
T738	4068-4071	CHEBI_SO_EXT:DNA	denotes	DNA
T739	4099-4106	CHEBI_EXT:thymine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	thymine
T740	4107-4116	SO_EXT:biological_sequence	denotes	sequences
T741	4159-4162	GO:0042555	denotes	Mcm
T742	4163-4171	GO_EXT:0004386	denotes	helicase
T743	4228-4237	NCBITaxon:40674	denotes	mammalian
T744	4238-4242	PR_EXT:000010246	denotes	Mcm4
T745	4238-4246	GO:0097373	denotes	Mcm4/6/7
T746	4257-4262	CHEMINF_GO_EXT:chemical_binding_or_bond_formation	denotes	binds
T747	4266-4285	CHEBI_SO_EXT:single_stranded_DNA	denotes	single-stranded DNA
T748	4317-4325	GO_EXT:0004386	denotes	helicase
T749	4355-4362	CHEBI_EXT:thymine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	thymine
T750	4378-4393	SO:0000984	denotes	single-stranded
T751	4417-4421	PR_EXT:000010246	denotes	Mcm4
T752	4417-4425	GO:0097373	denotes	Mcm4/6/7
T753	4426-4434	GO_EXT:0004386	denotes	helicase
T754	4487-4493	SO:0000985	denotes	duplex
T755	4574-4585	GO:0006260	denotes	replication
T756	4600-4606	SO_EXT:0001026	denotes	genome
T1431	4786-4796	CHEBI_SO_EXT:nucleotide	denotes	nucleotide
T1432	4805-4812	GO_PR_EXT:deoxyribonuclease_I	denotes	DNase I
T1433	4855-4863	GO_EXT:0004518	denotes	Nuclease
T1435	4904-4912	GO:0042571	denotes	antibody
T1436	4914-4921	CHEBI:2511	denotes	agarose
T1437	4931-4938	CHEBI_SO_EXT:peptide_or_peptide_region	denotes	peptide
T1438	4956-4960	NCBITaxon:10633	denotes	SV40
T1439	4963-4970	CHEBI_EXT:59132	denotes	antigen
T1440	4989-5000	NCBITaxon:10442	denotes	baculovirus
T1441	5010-5016	NCBITaxon:6960	denotes	insect
T1442	5017-5022	CL_GO_EXT:cell	denotes	cells
T1443	5041-5045	PR_EXT:000023591	denotes	PriA
T1444	5046-5054	GO_EXT:0004386	denotes	helicase
T1445	5055-5062	CHEBI_PR_EXT:protein	denotes	protein
T1446	5100-5116	CHEBI_SO_EXT:oligonucleotide	denotes	Oligonucleotides
T1447	5210-5224	CHEBI_EXT:polyacrylamide	denotes	polyacrylamide
T1635	5303-5313	GO:0010467	denotes	Expression
T1636	5334-5339	NCBITaxon:10088	denotes	mouse
T1637	5340-5344	PR_EXT:000010246	denotes	Mcm4
T1638	5340-5356	GO:0097373	denotes	Mcm4/6/7 complex
T1639	5361-5372	GO_SO_EXT:sequence_rearrangement_process	denotes	recombinant
T1640	5373-5386	NCBITaxon:10442	denotes	baculoviruses
T1641	5387-5397	GO:0010467	denotes	expressing
T1642	5398-5401	CHEBI_SO_EXT:histidine	denotes	His
T1643	5403-5407	PR_EXT:000010246	denotes	Mcm4
T1644	5408-5412	PR_EXT:000010248	denotes	Mcm6
T1645	5416-5420	PR_EXT:000010249	denotes	Mcm7
T1646	5426-5434	CHEBI_PR_EXT:protein	denotes	proteins
T1647	5485-5491	NCBITaxon:6960	denotes	insect
T1648	5492-5497	CL_GO_EXT:cell	denotes	cells
T1649	5570-5574	CL_GO_EXT:cell	denotes	CELL
T1650	5623-5633	GO:0010467	denotes	expression
T1651	5641-5645	PR_EXT:000010246	denotes	Mcm4
T1652	5641-5649	GO:0097373	denotes	Mcm4/6/7
T1653	5650-5658	CHEBI_PR_EXT:protein	denotes	proteins
T1654	5670-5675	CL_GO_EXT:cell	denotes	cells
T1655	5697-5708	GO_SO_EXT:sequence_rearrangement_process	denotes	recombinant
T1656	5709-5722	NCBITaxon:10442	denotes	baculoviruses
T1657	5723-5733	GO:0010467	denotes	expressing
T1658	5738-5741	CHEBI_SO_EXT:histidine	denotes	His
T1659	5743-5747	PR_EXT:000010246	denotes	Mcm4
T1660	5748-5752	PR_EXT:000010248	denotes	Mcm6
T1661	5753-5761	CHEBI_PR_EXT:protein	denotes	proteins
T1662	5772-5782	GO:0010467	denotes	expressing
T1663	5787-5791	PR_EXT:000010249	denotes	Mcm7
T1664	5845-5856	GO_SO_EXT:sequence_rearrangement_process	denotes	recombinant
T1665	5857-5861	PR_EXT:000010246	denotes	Mcm4
T1666	5857-5875	GO:0097373	denotes	Mcm4/6/7 complexes
T1897	5921-5924	CHEBI_SO_EXT:DNA	denotes	DNA
T1898	5940-5949	SO_EXT:biological_sequence	denotes	sequences
T1899	5962-5978	CHEBI_SO_EXT:oligonucleotide	denotes	oligonucleotides
T1900	6005-6008	CHEBI_SO_EXT:DNA	denotes	DNA
T1901	6068-6074	SO:0000985	denotes	duplex
T1902	6106-6115	GO:0097617	denotes	annealing
T1903	6116-6123	CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process	denotes	labeled
T1904	6124-6140	CHEBI_SO_EXT:oligonucleotide	denotes	oligonucleotides
T1905	6154-6169	_FRAGMENT	denotes	single-stranded
T1906	6179-6182	CHEBI_SO_EXT:single_stranded_DNA	denotes	DNA
T1907	6170-6178	SO:0000988	denotes	circular
T1908	6206-6213	CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process	denotes	labeled
T1909	6313-6316	CHEBI_SO_EXT:DNA	denotes	DNA
T1910	6383-6399	CHEBI_SO_EXT:oligonucleotide	denotes	oligonucleotides
T1911	6427-6436	SO_EXT:biological_sequence	denotes	sequences
T1912	6459-6463	GO:0005657	denotes	fork
T1913	6492-6501	GO:0097617	denotes	annealing
T1914	6506-6522	CHEBI_SO_EXT:oligonucleotide	denotes	oligonucleotides
T1915	6573-6577	GO:0005657	denotes	fork
T1916	6582-6585	_FRAGMENT	denotes	3′-
T1917	6592-6601	GO:0006272	denotes	extension
T1918	6589-6601	GO:0006273	denotes	5′-extension
T1919	6638-6654	CHEBI_SO_EXT:oligonucleotide	denotes	oligonucleotides
T1920	6677-6685	GO:0097617	denotes	annealed
T1921	6694-6702	PR_EXT:000009861	denotes	lamin B2
T1922	6718-6719	CHEBI_EXT:thymine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	T
T1923	6739-6747	PR_EXT:000009861	denotes	lamin B2
T1924	6748-6754	SO_EXT:0000296	denotes	origin
T1925	6830-6835	PR_EXT:000000084	denotes	c-myc
T1926	6843-6848	PR_EXT:000000084	denotes	c-myc
T1927	6865-6874	SO_EXT:biological_sequence	denotes	sequences
T1928	6914-6925	CHEBI_SO_EXT:nucleotide	denotes	nucleotides
T1929	7015-7021	GO:0032508	denotes	melted
T1930	7050-7057	CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process	denotes	labeled
T1931	7058-7073	CHEBI_SO_EXT:oligonucleotide	denotes	oligonucleotide
T1932	7087-7095	GO:0097617	denotes	annealed
T1933	7107-7114	CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process	denotes	labeled
T1934	7115-7130	CHEBI_SO_EXT:oligonucleotide	denotes	oligonucleotide
T1935	7154-7161	CHEBI:60004	denotes	mixture
T1936	7187-7191	CHEBI:9754	denotes	Tris
T1937	7192-7195	CHEBI:17883	denotes	HCl
T1938	7212-7217	CHEBI:6636	denotes	MgCl2
T1939	7228-7232	CHEBI:26710	denotes	NaCl
T1940	7373-7387	CHEBI_EXT:polyacrylamide	denotes	polyacrylamide
T1941	7411-7417	CHEBI_CHMO_EXT:buffer_solution	denotes	buffer
T1942	7442-7448	SO:0000985	denotes	duplex
T1943	7465-7480	_FRAGMENT	denotes	single-stranded
T1944	7490-7493	CHEBI_SO_EXT:single_stranded_DNA	denotes	DNA
T1945	7481-7489	SO:0000988	denotes	circular
T1946	7517-7523	SO:0000985	denotes	duplex
T1947	7559-7562	CHEBI_SO_EXT:DNA	denotes	DNA
T1948	7618-7629	GO:0097617	denotes	hybridizing
T1949	7664-7670	SO_EXT:0000440	denotes	vector
T1950	7672-7680	GO:0097617	denotes	annealed
T1951	7688-7703	_FRAGMENT	denotes	single-stranded
T1952	7713-7716	CHEBI_SO_EXT:single_stranded_DNA	denotes	DNA
T1953	7704-7712	SO:0000988	denotes	circular
T1954	7718-7721	CHEBI_SO_EXT:DNA	denotes	DNA
T1955	7781-7784	CHEBI:37972	denotes	32P
T1956	7785-7789	CHEBI_EXT:dGTP	denotes	dGTP
T1957	7850-7855	CHEBI_EXT:dNTP	denotes	dNTPs
T1958	7892-7899	CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process	denotes	labeled
T1959	7900-7906	SO:0000985	denotes	duplex
T1960	8011-8015	PR_EXT:000010246	denotes	Mcm4
T1961	8011-8027	GO:0097373	denotes	Mcm4/6/7 complex
T1962	8056-8063	CHEBI:60004	denotes	mixture
T1963	8130-8134	CHEBI_EXT:EDTA	denotes	EDTA
T1964	8144-8147	CHEBI:8984	denotes	SDS
T1965	8164-8174	GO_EXT:0008233	denotes	proteinase
T1966	8253-8267	CHEBI_EXT:polyacrylamide	denotes	polyacrylamide
T1967	8306-8321	_FRAGMENT	denotes	single-stranded
T1968	8331-8335	CHEBI_SO_EXT:single_stranded_DNA	denotes	DNAs
T1969	8322-8330	SO:0000988	denotes	circular
T1970	8366-8367	CHEBI_EXT:guanine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	G
T1971	8404-8413	SO_EXT:sequence_insertion_process	denotes	inserting
T1972	8416-8417	CHEBI_EXT:guanine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	G
T1973	8423-8429	SO_EXT:sequence_repeat_unit_or_region	denotes	repeat
T1974	8430-8438	SO_EXT:biological_sequence	denotes	sequence
T1975	8467-8476	GO:0097617	denotes	annealing
T1976	8484-8500	CHEBI_SO_EXT:oligonucleotide	denotes	oligonucleotides
T1977	8564-8569	PR_EXT:P23940	denotes	BamHI
T1978	8594-8603	SO_EXT:biological_sequence	denotes	sequences
T1979	8611-8617	SO_EXT:0000667	denotes	insert
T2602	8635-8638	CHEBI_SO_EXT:DNA	denotes	DNA
T2603	8635-8647	GO_EXT:0003678	denotes	DNA helicase
T2604	8690-8694	PR_EXT:000010246	denotes	Mcm4
T2605	8690-8698	GO:0097373	denotes	Mcm4/6/7
T2606	8699-8707	CHEBI_PR_EXT:protein	denotes	proteins
T2607	8754-8762	CHEBI:60004	denotes	mixtures
T2608	8788-8793	CHEBI:46756	denotes	HEPES
T2609	8794-8798	CHEBI:32145	denotes	NaOH
T2610	8815-8829	CHEBI:32954	denotes	sodium acetate
T2611	8837-8848	CHEBI:62964	denotes	Mg(CH3COO)2
T2612	8855-8858	CHEBI:18320	denotes	DTT
T2613	8883-8890	CHEBI:27575	denotes	ATP-γ-S
T2614	8895-8902	CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process	denotes	labeled
T2615	8969-8977	CHEBI:17754	denotes	glycerol
T2616	8992-9000	CHEBI:60004	denotes	mixtures
T2617	9028-9042	CHEBI_EXT:polyacrylamide	denotes	polyacrylamide
T2618	9063-9080	CHEBI:62964	denotes	magnesium acetate
T2619	9088-9096	CHEBI:17754	denotes	glycerol
T2620	9169-9172	CHEBI_SO_EXT:DNA	denotes	DNA
T2621	9169-9181	GO_EXT:0003678	denotes	DNA helicase
T2622	9203-9211	CHEBI:60004	denotes	mixtures
T2623	9305-9308	CHEBI_EXT:ATP	denotes	ATP
T2624	9428-9432	CHEBI_EXT:EDTA	denotes	EDTA
T2625	9442-9445	CHEBI:8984	denotes	SDS
T2626	9462-9472	GO_EXT:0008233	denotes	proteinase
T2627	9570-9580	GO_EXT:macromolecule_denaturation	denotes	denaturing
T2628	9581-9595	CHEBI_EXT:polyacrylamide	denotes	polyacrylamide
T2926	9612-9620	GO_EXT:0004518	denotes	Nuclease
T2927	9652-9660	CHEBI:60004	denotes	mixtures
T2928	9685-9690	CHEBI:46756	denotes	HEPES
T2929	9691-9695	CHEBI:32145	denotes	NaOH
T2930	9712-9726	CHEBI:32954	denotes	sodium acetate
T2931	9734-9745	CHEBI:62964	denotes	Mg(CH3COO)2
T2932	9752-9755	CHEBI:18320	denotes	DTT
T2933	9780-9787	CHEBI:27575	denotes	ATP-γ-S
T2934	9796-9801	CHEBI:3312	denotes	CaCl2
T2935	9814-9817	CHEBI:37972	denotes	32P
T2936	9818-9825	CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process	denotes	labeled
T2937	9872-9876	PR_EXT:000010246	denotes	Mcm4
T2938	9872-9880	GO:0097373	denotes	Mcm4/6/7
T2939	9881-9889	CHEBI_PR_EXT:protein	denotes	proteins
T2940	9952-9959	GO_PR_EXT:deoxyribonuclease_I	denotes	DNase I
T2941	10006-10014	CHEBI:60004	denotes	mixtures
T2942	10061-10068	GO_PR_EXT:deoxyribonuclease_I	denotes	DNase I
T2943	10119-10127	CHEBI:75958	denotes	solution
T2944	10135-10139	CHEBI_EXT:EDTA	denotes	EDTA
T2945	10146-10161	CHEBI:26710	denotes	sodium chloride
T2946	10166-10169	CHEBI:8984	denotes	SDS
T2947	10185-10190	NCBITaxon_EXT:yeast	denotes	yeast
T2948	10191-10194	CHEBI_SO_EXT:RNA	denotes	RNA
T2949	10201-10209	GO_EXT:0004518	denotes	nuclease
T2950	10233-10240	CHEMINF_GO_EXT:chemical_binding_or_bond_formation	denotes	binding
T2951	10306-10311	CHEBI:3312	denotes	CaCl2
T2952	10328-10336	GO_EXT:0004518	denotes	nuclease
T2953	10410-10418	MOP:0000780	denotes	Cleavage
T2954	10461-10465	CHEBI_EXT:EDTA	denotes	EDTA
T2955	10476-10480	CHEBI:32145	denotes	NaOH
T2956	10486-10493	CHEBI:60004	denotes	mixture
T2957	10580-10594	CHEBI:32954	denotes	sodium acetate
T2958	10605-10613	CHEBI_PR_EXT:protein	denotes	Proteins
T2959	10630-10636	CHEBI:15882	denotes	phenol
T2960	10637-10647	CHEBI:35255	denotes	chloroform
T2961	10664-10668	CHEBI_SO_EXT:DNA	denotes	DNAs
T2962	10687-10694	CHEBI:16236	denotes	ethanol
T2963	10735-10742	CHEBI:16236	denotes	ethanol
T2964	10757-10760	CHEBI_SO_EXT:DNA	denotes	DNA
T2965	10814-10828	CHEBI_EXT:polyacrylamide	denotes	polyacrylamide
T2966	10859-10863	CHEBI:16199	denotes	urea
T3352	10904-10908	PR_EXT:000010246	denotes	Mcm4
T3353	10904-10920	GO:0097373	denotes	Mcm4/6/7 complex
T3354	10950-10969	CHEBI_SO_EXT:single_stranded_DNA	denotes	single-stranded DNA
T3355	10978-10982	PR_EXT:000010246	denotes	Mcm4
T3356	10978-10986	GO:0097373	denotes	Mcm4/6/7
T3357	10987-10992	CHEMINF_GO_EXT:chemical_binding_or_bond_formation	denotes	binds
T3358	11005-11008	CHEBI_SO_EXT:DNA	denotes	DNA
T3359	11036-11042	SO:0000985	denotes	duplex
T3360	11069-11084	SO:0000984	denotes	single-stranded
T3361	11102-11109	CHEBI_EXT:thymine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	thymine
T3362	11158-11165	CHEMINF_GO_EXT:chemical_binding_or_bond_formation	denotes	binding
T3363	11169-11173	PR_EXT:000010246	denotes	Mcm4
T3364	11169-11177	GO:0097373	denotes	Mcm4/6/7
T3365	11188-11192	CHEBI_SO_EXT:DNA	denotes	DNAs
T3366	11207-11215	GO_EXT:0004518	denotes	nuclease
T3367	11240-11247	CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process	denotes	labeled
T3368	11265-11269	CHEBI_SO_EXT:DNA	denotes	DNAs
T3369	11274-11278	PR_EXT:000010246	denotes	Mcm4
T3370	11274-11282	GO:0097373	denotes	Mcm4/6/7
T3371	11302-11309	CHEBI:27575	denotes	ATP-γ-S
T3372	11324-11331	CHEMINF_GO_EXT:chemical_binding_or_bond_formation	denotes	binding
T3373	11345-11348	CHEBI_SO_EXT:DNA	denotes	DNA
T3374	11362-11370	GO_EXT:biological_recruitment	denotes	mobilize
T3375	11375-11383	GO_EXT:0004386	denotes	helicase
T3376	11416-11423	GO_PR_EXT:deoxyribonuclease_I	denotes	DNase I
T3377	11432-11441	MOP:0000780	denotes	cleavages
T3378	11466-11468	SO_EXT:0000028	denotes	bp
T3379	11469-11475	SO:0000985	denotes	duplex
T3380	11531-11540	MOP:0000780	denotes	cleavages
T3381	11570-11585	SO:0000984	denotes	single-stranded
T3382	11624-11631	GO_PR_EXT:deoxyribonuclease_I	denotes	DNase I
T3383	11635-11654	CHEBI_SO_EXT:double_stranded_DNA	denotes	double-stranded DNA
T3384	11671-11672	CHEBI_EXT:thymine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	T
T3385	11734-11749	SO:0000984	denotes	single-stranded
T3386	11822-11826	PR_EXT:000010246	denotes	Mcm4
T3387	11822-11830	GO:0097373	denotes	Mcm4/6/7
T3388	11865-11869	PR_EXT:000010246	denotes	Mcm4
T3389	11865-11873	GO:0097373	denotes	Mcm4/6/7
T3390	11877-11878	CHEBI_EXT:thymine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	T
T3391	11891-11900	SO_EXT:biological_sequence	denotes	sequences
T3392	11975-11994	CHEBI_SO_EXT:single_stranded_DNA	denotes	single-stranded DNA
T3393	12011-12020	GO_EXT:0004518	denotes	nucleases
T3394	12055-12074	CHEBI_SO_EXT:single_stranded_DNA	denotes	single-stranded DNA
T3395	12106-12117	GO:0042555	denotes	Mcm complex
T3396	12140-12143	CHEBI_SO_EXT:DNA	denotes	DNA
T3397	12152-12159	CHEMINF_GO_EXT:chemical_binding_or_bond_formation	denotes	binding
T3398	12183-12198	SO:0000984	denotes	single-stranded
T3399	12230-12234	PR_EXT:000010246	denotes	Mcm4
T3400	12230-12238	GO:0097373	denotes	Mcm4/6/7
T3401	12395-12399	CHEBI_SO_EXT:base	denotes	base
T3402	12395-12404	SO_EXT:0000028	denotes	base-pair
T3403	12405-12411	SO:0000985	denotes	duplex
T3404	12572-12576	PR_EXT:000010246	denotes	Mcm4
T3405	12572-12580	GO:0097373	denotes	Mcm4/6/7
T3406	12585-12589	CHEMINF_GO_EXT:chemical_binding_or_bond_formation	denotes	bind
T3407	12595-12606	GO:0006260	denotes	replication
T3408	12652-12666	GO_EXT:double_hexameric_macromolecular_complex	denotes	double hexamer
T3409	12725-12729	NCBITaxon:10633	denotes	SV40
T3410	12732-12739	CHEBI_EXT:59132	denotes	antigen
T3411	12746-12754	GO_EXT:0004518	denotes	Nuclease
T3412	12809-12813	GO:0005657	denotes	fork
T3413	12850-12865	SO:0000984	denotes	single-stranded
T3414	12893-12895	SO_EXT:0000028	denotes	bp
T3415	12896-12906	CHEBI_SO_EXT:double_stranded_DNA	denotes	duplex DNA
T3416	12923-12929	SO:0000985	denotes	duplex
T3417	12933-12946	SO:0000984	denotes	single-strand
T3418	12974-12977	GO:0042555	denotes	Mcm
T3419	12978-12985	CHEMINF_GO_EXT:chemical_binding_or_bond_formation	denotes	binding
T3420	12991-12997	GO_PR_EXT:deoxyribonuclease_I	denotes	DNaseI
T3421	13037-13041	PR_EXT:000010246	denotes	Mcm4
T3422	13037-13045	GO:0097373	denotes	Mcm4/6/7
T3423	13046-13053	CHEBI_PR_EXT:protein	denotes	protein
T3424	13079-13098	CHEBI_SO_EXT:single_stranded_DNA	denotes	single-stranded DNA
T3425	13146-13152	SO:0000985	denotes	duplex
T3426	13190-13201	GO:0006260	denotes	replication
T3427	13190-13206	GO:0005657	denotes	replication fork
T4006	13209-13221	_FRAGMENT	denotes	Formation of
T4007	13248-13257	GO:0065003	denotes	complexes
T4008	13222-13238	_FRAGMENT	denotes	double hexameric
T4009	13248-13257	GO_EXT:double_hexameric_macromolecular_complex	denotes	complexes
T4010	13239-13243	PR_EXT:000010246	denotes	Mcm4
T4011	13239-13247	GO:0097373	denotes	Mcm4/6/7
T4012	13270-13278	GO_EXT:0004386	denotes	helicase
T4013	13339-13343	PR_EXT:000010246	denotes	Mcm4
T4014	13339-13347	GO:0097373	denotes	Mcm4/6/7
T4015	13360-13384	GO_EXT:double_hexameric_macromolecular_complex	denotes	double hexameric complex
T4016	13403-13410	GO_EXT:hexameric_macromolecular_complex	denotes	hexamer
T4017	13473-13488	SO:0000984	denotes	single-stranded
T4018	13521-13536	SO:0000984	denotes	single-stranded
T4019	13608-13619	GO:0042555	denotes	Mcm complex
T4020	13660-13664	PR_EXT:000010246	denotes	Mcm4
T4021	13660-13668	GO:0097373	denotes	Mcm4/6/7
T4022	13684-13708	GO_EXT:hexameric_macromolecular_complex	denotes	single hexameric complex
T4023	13750-13752	CHEBI_SO_EXT:nucleotide	denotes	nt
T4024	13766-13781	SO:0000984	denotes	single-stranded
T4025	13797-13821	GO_EXT:double_hexameric_macromolecular_complex	denotes	double hexameric complex
T4026	13835-13838	CHEBI_SO_EXT:DNA	denotes	DNA
T4027	13895-13909	GO_EXT:double_hexameric_macromolecular_complex	denotes	double-hexamer
T4028	14055-14063	SO:0000984	denotes	unpaired
T4029	14098-14105	CHEMINF_GO_EXT:chemical_binding_or_bond_formation	denotes	binding
T4030	14110-14118	GO_EXT:0004386	denotes	helicase
T4031	14133-14137	PR_EXT:000010246	denotes	Mcm4
T4032	14133-14141	GO:0097373	denotes	Mcm4/6/7
T4033	14177-14181	PR_EXT:000010246	denotes	Mcm4
T4034	14177-14185	GO:0097373	denotes	Mcm4/6/7
T4035	14207-14219	CHEBI_EXT:radiolabel_process	denotes	radiolabeled
T4036	14273-14280	CHEBI:27575	denotes	ATP-γ-S
T4037	14290-14297	GO:0032991	denotes	complex
T4038	14290-14307	GO:0065003	denotes	complex formation
T4039	14334-14342	CHEBI:60004	denotes	mixtures
T4040	14361-14364	CHEBI_SO_EXT:DNA	denotes	DNA
T4041	14365-14372	CHEMINF_GO_EXT:chemical_binding_or_bond_formation	denotes	binding
T4042	14471-14474	CHEBI_EXT:ATP	denotes	ATP
T4043	14486-14489	CHEBI_SO_EXT:DNA	denotes	DNA
T4044	14486-14498	GO_EXT:0003678	denotes	DNA helicase
T4045	14558-14567	GO:0032991	denotes	complexes
T4046	14573-14577	PR_EXT:000010246	denotes	Mcm4
T4047	14573-14581	GO:0097373	denotes	Mcm4/6/7
T4048	14673-14680	GO_EXT:hexameric_macromolecular_complex	denotes	hexamer
T4049	14684-14688	PR_EXT:000010246	denotes	Mcm4
T4050	14684-14692	GO:0097373	denotes	Mcm4/6/7
T4051	14727-14736	GO:0032991	denotes	complexes
T4052	14750-14764	GO_EXT:double_hexameric_macromolecular_complex	denotes	double hexamer
T4053	14821-14828	GO_EXT:hexameric_macromolecular_complex	denotes	hexamer
T4054	14865-14889	GO_EXT:double_hexameric_macromolecular_complex	denotes	double hexamer complexes
T4055	14952-14976	GO_EXT:hexameric_macromolecular_complex	denotes	single hexamer complexes
T4056	15018-15033	SO:0000984	denotes	single-stranded
T4057	15057-15059	CHEBI_SO_EXT:nucleotide	denotes	nt
T4058	15106-15122	GO_EXT:double_hexameric_macromolecular_complex	denotes	double-hexameric
T4059	15123-15126	GO:0042555	denotes	Mcm
T4060	15127-15135	GO_EXT:0004386	denotes	helicase
T4061	15162-15165	CHEBI_SO_EXT:DNA	denotes	DNA
T4062	15162-15174	GO_EXT:0003678	denotes	DNA helicase
T4063	15183-15187	PR_EXT:000010246	denotes	Mcm4
T4064	15183-15199	GO:0097373	denotes	Mcm4/6/7 complex
T4065	15213-15221	GO_EXT:0004386	denotes	helicase
T4066	15339-15354	SO:0000984	denotes	single-stranded
T4067	15382-15384	CHEBI_SO_EXT:nucleotide	denotes	nt
T4068	15486-15501	SO:0000984	denotes	single-stranded
T4069	15525-15527	CHEBI_SO_EXT:nucleotide	denotes	nt
T4070	15559-15567	GO_EXT:0004386	denotes	helicase
T4071	15684-15688	PR_EXT:000010246	denotes	Mcm4
T4072	15684-15700	GO:0097373	denotes	Mcm4/6/7 complex
T4073	15714-15717	CHEBI_SO_EXT:DNA	denotes	DNA
T4074	15723-15747	GO_EXT:double_hexameric_macromolecular_complex	denotes	double hexameric complex
T4075	15773-15779	SO:0000985	denotes	duplex
T4076	15804-15807	CHEBI_SO_EXT:DNA	denotes	DNA
T4691	15826-15833	CHEBI_EXT:thymine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	thymine
T4692	15839-15854	SO:0000984	denotes	single-stranded
T4693	15891-15903	_FRAGMENT	denotes	unwinding of
T4694	15911-15914	GO:0032508	denotes	DNA
T4695	15911-15914	CHEBI_SO_EXT:DNA	denotes	DNA
T4696	15918-15922	PR_EXT:000010246	denotes	Mcm4
T4697	15918-15926	GO:0097373	denotes	Mcm4/6/7
T4698	15937-15944	CHEMINF_GO_EXT:chemical_binding_or_bond_formation	denotes	binding
T4699	15952-15956	PR_EXT:000010246	denotes	Mcm4
T4700	15952-15960	GO:0097373	denotes	Mcm4/6/7
T4701	15961-15975	GO_EXT:double_hexameric_macromolecular_complex	denotes	double hexamer
T4702	16005-16012	GO_EXT:hexameric_macromolecular_complex	denotes	hexamer
T4703	16013-16022	GO_MOP_EXT:catalysis	denotes	catalyzes
T4704	16051-16057	SO:0000985	denotes	duplex
T4705	16104-16112	GO_EXT:hexameric_macromolecular_complex	denotes	hexamers
T4706	16227-16231	PR_EXT:000010246	denotes	Mcm4
T4707	16227-16235	GO:0097373	denotes	Mcm4/6/7
T4708	16245-16252	CHEBI_EXT:thymine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	thymine
T4709	16286-16301	SO:0000984	denotes	single-stranded
T4710	16349-16350	CHEBI_EXT:thymine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	T
T4711	16351-16352	CHEBI_EXT:guanine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	G
T4712	16382-16383	CHEBI_EXT:thymine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	T
T4713	16404-16405	CHEBI_EXT:thymine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	T
T4714	16436-16437	CHEBI_EXT:guanine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	G
T4715	16458-16459	CHEBI_EXT:guanine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	G
T4716	16476-16484	GO_EXT:0004386	denotes	helicase
T4717	16533-16537	PR_EXT:000010246	denotes	Mcm4
T4718	16533-16541	GO:0097373	denotes	Mcm4/6/7
T4719	16612-16613	CHEBI_EXT:thymine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	T
T4720	16638-16642	PR_EXT:000010246	denotes	Mcm4
T4721	16638-16646	GO:0097373	denotes	Mcm4/6/7
T4722	16651-16655	CHEMINF_GO_EXT:chemical_binding_or_bond_formation	denotes	bind
T4723	16665-16666	CHEBI_EXT:thymine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	T
T4724	16667-16668	CHEBI_EXT:guanine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	G
T4725	16686-16710	GO_EXT:double_hexameric_macromolecular_complex	denotes	double hexameric complex
T4726	16794-16802	GO_EXT:0004386	denotes	helicase
T4727	16830-16837	GO_EXT:hexameric_macromolecular_complex	denotes	hexamer
T4728	16859-16866	CHEBI_EXT:thymine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	thymine
T4729	16906-16913	GO_EXT:hexameric_macromolecular_complex	denotes	hexamer
T4730	16944-16950	SO:0000985	denotes	duplex
T4731	17001-17005	GO:0005657	denotes	fork
T4732	17077-17078	CHEBI_EXT:guanine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	G
T4733	17282-17291	CHEBI:36357	denotes	molecules
T4734	17299-17303	PR_EXT:000010246	denotes	Mcm4
T4735	17299-17307	GO:0097373	denotes	Mcm4/6/7
T4736	17308-17316	GO_EXT:hexameric_macromolecular_complex	denotes	hexamers
T5190	17348-17355	CHEMINF_GO_EXT:chemical_binding_or_bond_formation	denotes	Binding
T5191	17360-17368	GO_EXT:0004386	denotes	helicase
T5192	17380-17384	PR_EXT:000010246	denotes	Mcm4
T5193	17380-17388	GO:0097373	denotes	Mcm4/6/7
T5194	17392-17398	GO:0005657	denotes	forked
T5195	17403-17406	_FRAGMENT	denotes	3′-
T5196	17413-17422	GO:0006272	denotes	extension
T5197	17410-17422	GO:0006273	denotes	5′-extension
T5198	17489-17497	GO_EXT:0004386	denotes	helicase
T5199	17511-17517	GO:0005657	denotes	forked
T5200	17532-17536	GO:0005657	denotes	fork
T5201	17587-17591	GO:0005657	denotes	fork
T5202	17624-17628	GO:0005657	denotes	fork
T5203	17654-17658	GO:0005657	denotes	fork
T5204	17679-17691	GO:0006273	denotes	5′-extension
T5205	17695-17707	GO:0006272	denotes	3′-extension
T5206	17737-17741	PR_EXT:000010246	denotes	Mcm4
T5207	17737-17745	GO:0097373	denotes	Mcm4/6/7
T5208	17746-17751	CHEMINF_GO_EXT:chemical_binding_or_bond_formation	denotes	bound
T5209	17757-17761	GO:0005657	denotes	fork
T5210	17771-17775	GO:0005657	denotes	fork
T5211	17824-17828	GO:0005657	denotes	fork
T5212	17874-17878	PR_EXT:000023591	denotes	PriA
T5213	17879-17887	GO_EXT:0004386	denotes	helicase
T5214	17908-17913	CHEMINF_GO_EXT:chemical_binding_or_bond_formation	denotes	bound
T5215	17919-17923	GO:0005657	denotes	fork
T5216	17955-17959	GO:0005657	denotes	fork
T5217	17997-18001	PR_EXT:000010246	denotes	Mcm4
T5218	17997-18005	GO:0097373	denotes	Mcm4/6/7
T5219	18006-18011	CHEMINF_GO_EXT:chemical_binding_or_bond_formation	denotes	bound
T5220	18020-18032	GO:0006273	denotes	5′-extension
T5221	18037-18049	GO:0006272	denotes	3′-extension
T5222	18103-18107	GO:0005657	denotes	fork
T5223	18118-18122	GO:0005657	denotes	fork
T5224	18140-18144	PR_EXT:000010246	denotes	Mcm4
T5225	18140-18148	GO:0097373	denotes	Mcm4/6/7
T5226	18149-18154	CHEMINF_GO_EXT:chemical_binding_or_bond_formation	denotes	bound
T5227	18160-18164	GO:0005657	denotes	fork
T5228	18206-18213	GO:0032991	denotes	complex
T5229	18275-18279	PR_EXT:000010246	denotes	Mcm4
T5230	18275-18291	GO:0097373	denotes	Mcm4/6/7 complex
T5231	18307-18310	CHEBI_SO_EXT:DNA	denotes	DNA
T5232	18350-18365	SO:0000984	denotes	single-stranded
T5233	18472-18491	CHEBI_SO_EXT:single_stranded_DNA	denotes	single-stranded DNA
T5234	18500-18504	GO:0005657	denotes	fork
T5235	18540-18544	GO:0005657	denotes	fork
T5236	18554-18558	GO:0005657	denotes	fork
T5237	18586-18590	PR_EXT:000010246	denotes	Mcm4
T5238	18586-18594	GO:0097373	denotes	Mcm4/6/7
T5239	18619-18627	GO_EXT:0004386	denotes	helicase
T5240	18641-18645	GO:0005657	denotes	fork
T5241	18670-18676	SO:0000985	denotes	duplex
T5242	18703-18722	CHEBI_SO_EXT:double_stranded_DNA	denotes	double-stranded DNA
T5243	18751-18757	GO_EXT:0016887	denotes	ATPase
T5244	18770-18774	PR_EXT:000010246	denotes	Mcm4
T5245	18770-18778	GO:0097373	denotes	Mcm4/6/7
T5246	18802-18806	PR_EXT:000023591	denotes	PriA
T5247	18829-18837	GO_EXT:0004386	denotes	helicase
T5248	18857-18862	CHEMINF_GO_EXT:chemical_binding_or_bond_formation	denotes	binds
T5249	18870-18874	GO:0005657	denotes	fork
T5250	18900-18904	GO:0005657	denotes	fork
T5251	18936-18939	CHEBI_SO_EXT:DNA	denotes	DNA
T5252	18945-18949	GO:0005657	denotes	fork
T5253	18998-19002	GO:0005657	denotes	fork
T5254	19068-19072	PR_EXT:000010246	denotes	Mcm4
T5255	19068-19084	GO:0097373	denotes	Mcm4/6/7 complex
T5256	19085-19090	CHEMINF_GO_EXT:chemical_binding_or_bond_formation	denotes	binds
T5257	19094-19113	CHEBI_SO_EXT:single_stranded_DNA	denotes	single-stranded DNA
T5258	19127-19135	GO_EXT:0004386	denotes	helicase
T5259	19175-19190	SO:0000984	denotes	single-stranded
T5866	19201-19213	GO:0006272	denotes	3′-extension
T5867	19224-19226	CHEBI_EXT:thymine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	dT
T5868	19249-19253	PR_EXT:000010246	denotes	Mcm4
T5869	19249-19257	GO:0097373	denotes	Mcm4/6/7
T5870	19288-19300	GO:0006272	denotes	3′-extension
T5871	19323-19331	NCBITaxon:2157	denotes	archaeal
T5872	19332-19335	GO:0042555	denotes	Mcm
T5873	19340-19347	GO_EXT:hexameric_macromolecular_complex	denotes	hexamer
T5874	19359-19366	_FRAGMENT	denotes	fission
T5875	19379-19384	NCBITaxon:4894	denotes	yeast
T5876	19371-19378	GO:0007114	denotes	budding
T5877	19371-19384	NCBITaxon:4892	denotes	budding yeast
T5878	19385-19398	GO:0042555	denotes	Mcm complexes
T5879	19431-19443	GO:0006272	denotes	3′-extension
T5880	19471-19473	CHEBI_EXT:thymine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	dT
T5881	19481-19483	CHEBI_EXT:deoxyadenosine_nucleoside_or_nucleotide_residue	denotes	dA
T5882	19491-19493	CHEBI_EXT:deoxycytidine_nucleoside_or_nucleotide_residue	denotes	dC
T5883	19504-19506	CHEBI_EXT:deoxyguanosine_nucleoside_or_nucleotide_residue	denotes	dG
T5884	19545-19550	NCBITaxon:10088	denotes	mouse
T5885	19551-19555	PR_EXT:000010246	denotes	Mcm4
T5886	19551-19559	GO:0097373	denotes	Mcm4/6/7
T5887	19611-19615	PR_EXT:000010246	denotes	Mcm4
T5888	19611-19619	GO:0097373	denotes	Mcm4/6/7
T5889	19620-19625	CHEMINF_GO_EXT:chemical_binding_or_bond_formation	denotes	binds
T5890	19629-19631	CHEBI_EXT:thymine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	dT
T5891	19634-19636	CHEBI_EXT:deoxycytidine_nucleoside_or_nucleotide_residue	denotes	dC
T5892	19642-19644	CHEBI_EXT:deoxyguanosine_nucleoside_or_nucleotide_residue	denotes	dG
T5893	19645-19657	GO:0006272	denotes	3′-extension
T5894	19688-19690	CHEBI_EXT:deoxyadenosine_nucleoside_or_nucleotide_residue	denotes	dA
T5895	19691-19703	GO:0006272	denotes	3′-extension
T5896	19792-19796	PR_EXT:000010246	denotes	Mcm4
T5897	19792-19800	GO:0097373	denotes	Mcm4/6/7
T5898	19801-19806	CHEMINF_GO_EXT:chemical_binding_or_bond_formation	denotes	binds
T5899	19816-19821	CHEBI_SO_EXT:oligonucleotide	denotes	oligo
T5900	19822-19824	CHEBI_EXT:thymine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	dT
T5901	19827-19829	CHEBI_EXT:deoxycytidine_nucleoside_or_nucleotide_residue	denotes	dC
T5902	19835-19837	CHEBI_EXT:deoxyguanosine_nucleoside_or_nucleotide_residue	denotes	dG
T5903	19858-19863	CHEBI_SO_EXT:oligonucleotide	denotes	oligo
T5904	19864-19866	CHEBI_EXT:deoxyadenosine_nucleoside_or_nucleotide_residue	denotes	dA
T5905	19887-19889	CHEBI_EXT:thymine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	dT
T5906	19897-19909	GO:0006272	denotes	3′-extension
T5907	19939-19944	PR_EXT:000010246	denotes	Mcm 4
T5908	19939-19942	_FRAGMENT	denotes	Mcm
T5909	19945-19946	PR_EXT:000010248	denotes	6
T5910	19947-19948	PR_EXT:000010249	denotes	7
T5911	19939-19948	GO:0097373	denotes	Mcm 4/6/7
T5912	20070-20075	NCBITaxon_EXT:yeast	denotes	yeast
T5913	20076-20080	PR_EXT:000010246	denotes	Mcm4
T5914	20076-20084	GO:0097373	denotes	Mcm4/6/7
T5915	20085-20094	GO_EXT:0004386	denotes	helicases
T5916	20130-20142	GO:0006272	denotes	3′-extension
T5917	20168-20170	CHEBI_EXT:thymine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	dT
T5918	20178-20190	GO:0006273	denotes	5′-extension
T5919	20201-20205	PR_EXT:000010246	denotes	Mcm4
T5920	20201-20209	GO:0097373	denotes	Mcm4/6/7
T5921	20216-20220	CHEMINF_GO_EXT:chemical_binding_or_bond_formation	denotes	bind
T5922	20247-20250	GO:0042555	denotes	Mcm
T5923	20251-20259	GO_EXT:0004386	denotes	helicase
T5924	20292-20294	CHEBI_EXT:thymine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	dT
T5925	20302-20314	GO:0006272	denotes	3′-extension
T5926	20404-20408	GO:0005657	denotes	fork
T5927	20435-20443	GO_EXT:0004386	denotes	helicase
T5928	20515-20524	NCBITaxon:40674	denotes	mammalian
T5929	20525-20528	GO:0042555	denotes	Mcm
T5930	20529-20537	GO_EXT:0004386	denotes	helicase
T5931	20568-20575	CHEBI_EXT:thymine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	thymine
T5932	20594-20609	SO:0000984	denotes	single-stranded
T6357	20636-20643	CHEBI_EXT:thymine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	thymine
T6358	20670-20673	GO:0042555	denotes	Mcm
T6359	20674-20682	GO_EXT:0004386	denotes	helicase
T6360	20767-20774	CHEBI_EXT:thymine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	thymine
T6361	20775-20783	CHEBI_EXT:residue	denotes	residues
T6362	20806-20809	GO:0042555	denotes	Mcm
T6363	20810-20818	GO_EXT:0004386	denotes	helicase
T6364	20845-20851	SO:0000985	denotes	duplex
T6365	20856-20875	CHEBI_SO_EXT:single_stranded_DNA	denotes	single-stranded DNA
T6366	20892-20894	SO_EXT:0000028	denotes	bp
T6367	20895-20901	SO:0000985	denotes	duplex
T6368	20946-20948	CHEBI_SO_EXT:nucleotide	denotes	nt
T6369	20973-20983	CHEBI_SO_EXT:nucleotide	denotes	nucleotide
T6370	21035-21040	NCBITaxon:10088	denotes	mouse
T6371	21041-21045	PR_EXT:000010246	denotes	Mcm4
T6372	21041-21049	GO:0097373	denotes	Mcm4/6/7
T6373	21105-21109	PR_EXT:000010246	denotes	Mcm4
T6374	21105-21113	GO:0097373	denotes	Mcm4/6/7
T6375	21135-21143	GO:0097617	denotes	annealed
T6376	21150-21165	CHEBI_SO_EXT:oligonucleotide	denotes	oligonucleotide
T6377	21214-21222	GO_EXT:0004386	denotes	helicase
T6378	21289-21296	SO_EXT:sequence_repeat_unit_or_region	denotes	repeats
T6379	21303-21315	CHEBI_SO_EXT:dinucleotide	denotes	dinucleotide
T6380	21353-21361	GO_EXT:0004386	denotes	helicase
T6381	21409-21416	CHEBI_EXT:thymine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	thymine
T6382	21555-21562	SO_EXT:sequence_repeat_unit_or_region	denotes	repeats
T6383	21569-21582	CHEBI_SO_EXT:dinucleotide	denotes	dinucleotides
T6384	21588-21595	CHEBI_EXT:thymine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	thymine
T6385	21633-21636	GO:0042555	denotes	Mcm
T6386	21637-21645	GO_EXT:0004386	denotes	helicase
T6387	21696-21702	GO:0097617	denotes	anneal
T6388	21741-21744	GO:0042555	denotes	Mcm
T6389	21891-21900	SO_EXT:sequence_repeat_unit_or_region	denotes	repeating
T6390	21901-21910	SO_EXT:biological_sequence	denotes	sequences
T6391	21914-21921	CHEBI_EXT:thymine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	thymine
T6392	21926-21934	CHEBI_EXT:cytosine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	cytosine
T6393	21962-21968	SO:0000985	denotes	duplex
T6394	22069-22073	PR_EXT:000010246	denotes	Mcm4
T6395	22069-22085	GO:0097373	denotes	Mcm4/6/7 complex
T6396	22155-22162	CHEBI_EXT:thymine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	thymine
T6397	22208-22211	GO:0042555	denotes	Mcm
T6398	22212-22220	GO_EXT:0004386	denotes	helicase
T6399	22321-22323	CHEBI_SO_EXT:nucleotide	denotes	nt
T6400	22439-22446	CHEBI_EXT:thymine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	thymine
T6401	22544-22551	CHEBI_EXT:thymine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	thymine
T6402	22591-22594	GO:0042555	denotes	Mcm
T6403	22595-22603	GO_EXT:0004386	denotes	helicase
T6936	22646-22655	SO_EXT:biological_sequence	denotes	sequences
T6937	22665-22670	PR_EXT:000000084	denotes	c-myc
T6938	22671-22677	SO_EXT:0000296	denotes	origin
T6939	22687-22690	GO:0042555	denotes	Mcm
T6940	22691-22699	GO_EXT:0004386	denotes	helicase
T6941	22741-22750	SO_EXT:biological_sequence	denotes	sequences
T6942	22772-22774	CHEBI_EXT:thymine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	dT
T6943	22801-22806	NCBITaxon:9606	denotes	human
T6944	22807-22815	PR_EXT:000009861	denotes	lamin B2
T6945	22816-22822	SO_EXT:0000296	denotes	origin
T6946	22828-22835	CHEBI_EXT:thymine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	thymine
T6947	22889-22892	GO:0042555	denotes	Mcm
T6948	22893-22901	GO_EXT:0004386	denotes	helicase
T6949	22919-22930	SO_EXT:sequence_substitution_entity_or_process	denotes	replacement
T6950	22934-22942	CHEBI_EXT:thymine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	thymines
T6951	22948-22956	CHEBI_EXT:guanine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	guanines
T6952	22971-22979	GO_EXT:0004386	denotes	helicase
T6953	23034-23045	GO:0006260	denotes	replication
T6954	23034-23053	SO_EXT:0000296	denotes	replication origins
T6955	23069-23076	CHEBI_EXT:thymine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	thymine
T6956	23114-23117	GO:0042555	denotes	Mcm
T6957	23118-23126	GO_EXT:0004386	denotes	helicase
T6958	23194-23199	PR_EXT:000000084	denotes	c-myc
T6959	23212-23221	SO_EXT:biological_sequence	denotes	sequences
T6960	23244-23247	CHEBI_SO_EXT:DNA	denotes	DNA
T6961	23244-23257	GO:0032508	denotes	DNA unwinding
T6962	23281-23286	NCBITaxon:9606	denotes	human
T6963	23287-23292	PR_EXT:000000084	denotes	c-myc
T6964	23293-23299	SO_EXT:0000296	denotes	origin
T6965	23323-23328	PR_EXT:000000084	denotes	c-myc
T6966	23329-23339	GO_EXT:DNA_replicator	denotes	replicator
T6967	23362-23370	SO:0000984	denotes	unpaired
T6968	23388-23393	PR_EXT:000000084	denotes	c-myc
T6969	23443-23448	PR_EXT:000000084	denotes	c-myc
T6970	23554-23559	PR_EXT:000000084	denotes	c-myc
T6971	23560-23566	SO_EXT:0000296	denotes	origin
T6972	23581-23589	SO_EXT:sequence_deletion_entity_or_process	denotes	deletion
T6973	23616-23626	GO_EXT:DNA_replicator	denotes	replicator
T6974	23642-23646	PR_EXT:000010246	denotes	Mcm4
T6975	23642-23650	GO:0097373	denotes	Mcm4/6/7
T6976	23665-23673	PR_EXT:000009861	denotes	lamin B2
T6977	23675-23682	CHEBI_EXT:thymine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	thymine
T6978	23695-23700	PR_EXT:000000084	denotes	c-myc
T6979	23721-23726	PR_EXT:000000084	denotes	c-myc
T6980	23796-23805	SO_EXT:biological_sequence	denotes	sequences
T6981	23815-23820	PR_EXT:000000084	denotes	c-myc
T6982	23821-23827	SO_EXT:0000296	denotes	origin
T6983	23874-23877	GO:0042555	denotes	Mcm
T6984	23878-23886	GO_EXT:0004386	denotes	helicase
T6985	23920-23929	SO_EXT:biological_sequence	denotes	sequences
T6986	23939-23946	CHEBI_EXT:thymine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	thymine
T6987	23968-23972	PR_EXT:000010246	denotes	Mcm4
T6988	23968-23976	GO:0097373	denotes	Mcm4/6/7
T6989	23992-23994	CHEBI_SO_EXT:nucleotide	denotes	nt
T6990	24068-24075	CHEBI_EXT:thymine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	thymine
T6991	24097-24105	SO_EXT:biological_sequence	denotes	sequence
T6992	24129-24132	GO:0042555	denotes	Mcm
T6993	24133-24141	GO_EXT:0004386	denotes	helicase
T6994	24175-24179	PR_EXT:000010246	denotes	MCM4
T6995	24175-24183	GO:0097373	denotes	MCM4/6/7
T6996	24184-24189	CHEMINF_GO_EXT:chemical_binding_or_bond_formation	denotes	bound
T6997	24247-24252	PR_EXT:000000084	denotes	c-myc
T6998	24270-24275	PR_EXT:000000084	denotes	c-myc
T6999	24320-24328	PR_EXT:000009861	denotes	lamin B2
T7000	24353-24361	GO_EXT:0004386	denotes	helicase
T7001	24366-24369	CHEBI_SO_EXT:DNA	denotes	DNA
T7002	24370-24377	CHEMINF_GO_EXT:chemical_binding_or_bond_formation	denotes	binding
T7003	24488-24495	CHEBI_EXT:guanine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	guanine
T7004	24500-24508	CHEBI_EXT:cytosine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	cytosine
T7005	24523-24527	CHEMINF_GO_EXT:chemical_binding_or_bond_formation	denotes	bind
T7006	24531-24534	GO:0042555	denotes	Mcm
T7007	24559-24567	GO_EXT:0004386	denotes	helicase
T7598	24575-24578	CHEBI_SO_EXT:DNA	denotes	DNA
T7599	24575-24588	GO:0032508	denotes	DNA unwinding
T7600	24592-24596	PR_EXT:000010246	denotes	Mcm4
T7601	24592-24600	GO:0097373	denotes	Mcm4/6/7
T7602	24629-24638	SO_EXT:biological_sequence	denotes	sequences
T7603	24646-24652	SO:0000985	denotes	duplex
T7604	24687-24694	CHEBI_EXT:thymine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	thymine
T7605	24700-24719	CHEBI_SO_EXT:single_stranded_DNA	denotes	single-stranded DNA
T7606	24774-24777	GO:0042555	denotes	Mcm
T7607	24778-24786	GO_EXT:0004386	denotes	helicase
T7608	24821-24828	CHEBI_EXT:thymine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	thymine
T7609	24829-24838	SO_EXT:biological_sequence	denotes	sequences
T7610	24867-24890	GO:0032508	denotes	unwinding of duplex DNA
T7611	24880-24890	CHEBI_SO_EXT:double_stranded_DNA	denotes	duplex DNA
T7612	24932-24942	CHEBI_SO_EXT:nucleotide	denotes	nucleotide
T7613	24962-24968	SO:0000985	denotes	duplex
T7614	25069-25073	GO:0005657	denotes	fork
T7615	25088-25092	GO:0005657	denotes	fork
T7616	25113-25122	SO_EXT:biological_sequence	denotes	sequences
T7617	25130-25136	SO:0000985	denotes	duplex
T7618	25178-25186	CHEBI_EXT:cytosine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	cytosine
T7619	25187-25195	CHEBI_EXT:residue	denotes	residues
T7620	25240-25244	PR_EXT:000010246	denotes	Mcm4
T7621	25240-25248	GO:0097373	denotes	Mcm4/6/7
T7622	25249-25254	CHEMINF_GO_EXT:chemical_binding_or_bond_formation	denotes	bound
T7623	25266-25270	GO:0005657	denotes	fork
T7624	25355-25359	PR_EXT:000010246	denotes	Mcm4
T7625	25355-25363	GO:0097373	denotes	Mcm4/6/7
T7626	25364-25369	CHEMINF_GO_EXT:chemical_binding_or_bond_formation	denotes	binds
T7627	25373-25388	SO:0000984	denotes	single-stranded
T7628	25408-25411	CHEBI_SO_EXT:DNA	denotes	DNA
T7629	25408-25420	GO_EXT:0003678	denotes	DNA helicase
T7630	25431-25435	GO:0005657	denotes	fork
T7631	25468-25472	PR_EXT:000010246	denotes	Mcm4
T7632	25468-25476	GO:0097373	denotes	Mcm4/6/7
T7633	25507-25511	NCBITaxon:10633	denotes	SV40
T7634	25514-25521	CHEBI_EXT:59132	denotes	antigen
T7635	25522-25525	CHEBI_SO_EXT:DNA	denotes	DNA
T7636	25522-25534	GO_EXT:0003678	denotes	DNA helicase
T7637	25553-25560	CHEBI_EXT:thymine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	thymine
T7638	25564-25571	CHEBI_EXT:adenine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	adenine
T7639	25575-25583	SO_EXT:sequence_insertion_process	denotes	inserted
T7640	25599-25609	CHEBI_SO_EXT:nucleotide	denotes	nucleotide
T7641	25611-25618	SO_EXT:sequence_repeat_unit_or_region	denotes	repeats
T7642	25672-25676	GO:0005657	denotes	fork
T7643	25695-25699	GO:0005657	denotes	fork
T7644	25800-25806	SO:0000985	denotes	duplex
T7645	25818-25822	GO:0005657	denotes	fork
T7646	25840-25844	GO:0005657	denotes	fork
T7647	25862-25866	GO:0005657	denotes	fork
T7648	25887-25891	GO:0005657	denotes	fork
T7649	25939-25945	SO:0000985	denotes	duplex
T7650	26082-26088	SO:0000985	denotes	duplex
T7651	26082-26092	CHEBI_SO_EXT:double_stranded_DNA	denotes	duplex DNA
T7652	26089-26092	CHEBI_SO_EXT:DNA	denotes	DNA
T7653	26098-26113	_FRAGMENT	denotes	single-stranded
T7654	26123-26126	CHEBI_SO_EXT:single_stranded_DNA	denotes	DNA
T7655	26114-26122	SO:0000988	denotes	circular
T7656	26184-26190	SO:0000985	denotes	duplex
T7657	26184-26194	CHEBI_SO_EXT:double_stranded_DNA	denotes	duplex DNA
T7658	26191-26194	CHEBI_SO_EXT:DNA	denotes	DNA
T7659	26217-26223	SO:0000985	denotes	duplex
T7660	26268-26274	SO_EXT:0000440	denotes	vector
T7661	26316-26318	CHEBI_SO_EXT:nucleotide	denotes	nt
T7662	26324-26325	CHEBI_EXT:guanine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	G
T7663	26339-26349	SO_EXT:sequence_downstreamness	denotes	downstream
T7664	26357-26368	GO:0097617	denotes	hybridizing
T7665	26369-26384	CHEBI_SO_EXT:oligonucleotide	denotes	oligonucleotide
T7666	26404-26408	PR_EXT:000010246	denotes	Mcm4
T7667	26404-26412	GO:0097373	denotes	Mcm4/6/7
T7668	26413-26421	GO_EXT:0004386	denotes	helicase
T7669	26435-26445	CHEBI_SO_EXT:double_stranded_DNA	denotes	duplex DNA
T7670	26456-26458	CHEBI_SO_EXT:nucleotide	denotes	nt
T7671	26705-26707	CHEBI_SO_EXT:nucleotide	denotes	nt
T7672	26786-26790	PR_EXT:000010246	denotes	Mcm4
T7673	26786-26802	GO:0097373	denotes	Mcm4/6/7 complex
T7674	26845-26849	GO:0005657	denotes	fork
T7675	26877-26883	SO:0000985	denotes	duplex
T7676	26927-26930	GO:0042555	denotes	Mcm
T7677	26931-26939	GO_EXT:0004386	denotes	helicase
T7678	26989-26996	CHEBI_EXT:thymine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	thymine
T7679	26997-27004	CHEBI_EXT:adenine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	adenine
T7680	27005-27010	SO_EXT:0000028	denotes	pairs
T7681	27043-27049	SO:0000985	denotes	duplex
T7682	27096-27100	PR_EXT:000010246	denotes	Mcm4
T7683	27096-27104	GO:0097373	denotes	Mcm4/6/7
T7684	27121-27125	PR_EXT:000010246	denotes	Mcm4
T7685	27121-27129	GO:0097373	denotes	Mcm4/6/7
T7686	27194-27198	CHEBI_SO_EXT:base	denotes	base
T7687	27194-27205	SO_EXT:0000028	denotes	base-paired
T7688	27284-27288	GO:0005657	denotes	fork
T7689	27310-27317	CHEBI_SO_EXT:inosine	denotes	inosine
T7690	27319-27320	CHEBI_SO_EXT:inosine	denotes	I
T7691	27322-27329	CHEBI_EXT:residue	denotes	residue
T7692	27341-27350	CHEBI_EXT:guanine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	guanosine
T7693	27351-27358	CHEBI_EXT:residue	denotes	residue
T7694	27389-27390	CHEBI_SO_EXT:inosine	denotes	I
T7695	27391-27392	CHEBI_EXT:cytosine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	C
T7696	27393-27397	CHEBI_SO_EXT:base	denotes	base
T7697	27393-27402	SO_EXT:0000028	denotes	base pair
T7698	27429-27430	CHEBI_EXT:guanine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	G
T7699	27431-27432	CHEBI_EXT:cytosine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	C
T7700	27433-27437	CHEBI_SO_EXT:base	denotes	base
T7701	27433-27442	SO_EXT:0000028	denotes	base pair
T7702	27446-27456	CHEBI_SO_EXT:double_stranded_DNA	denotes	duplex DNA
T7703	27476-27484	CHEBI_EXT:hydrogen	denotes	hydrogen
T7704	27513-27514	CHEBI_EXT:adenine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	A
T7705	27515-27516	CHEBI_EXT:thymine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	T
T7706	27517-27521	CHEBI_SO_EXT:base	denotes	base
T7707	27517-27526	SO_EXT:0000028	denotes	base pair
T7708	27561-27565	GO:0005657	denotes	fork
T7709	27632-27642	CHEBI_SO_EXT:double_stranded_DNA	denotes	duplex DNA
T7710	27664-27668	PR_EXT:000010246	denotes	Mcm4
T7711	27664-27672	GO:0097373	denotes	Mcm4/6/7
T7712	27697-27699	CHEBI_SO_EXT:nucleotide	denotes	nt
T7713	27723-27730	SO_EXT:sequence_repeat_unit_or_region	denotes	repeats
T7714	27746-27750	GO:0005657	denotes	fork
T7715	27812-27816	GO:0005657	denotes	fork
T7716	27887-27891	GO:0005657	denotes	fork
T7717	27948-27954	SO:0000985	denotes	duplex
T7718	27983-27987	CHEBI_SO_EXT:base	denotes	base
T7719	27983-27992	SO_EXT:0000028	denotes	base pair
T7720	28026-28030	PR_EXT:000010246	denotes	Mcm4
T7721	28026-28034	GO:0097373	denotes	Mcm4/6/7
T8768	28071-28074	CHEBI_SO_EXT:DNA	denotes	DNA
T8769	28071-28083	GO_EXT:0003678	denotes	DNA helicase
T8770	28121-28132	GO:0006260	denotes	replication
T8771	28121-28138	GO:0005657	denotes	replication forks
T8772	28199-28210	GO:0006260	denotes	replication
T8773	28199-28218	SO_EXT:0000296	denotes	replication origins
T8774	28258-28269	GO:0006260	denotes	replicative
T8775	28270-28279	GO_EXT:0004386	denotes	helicases
T8776	28294-28297	CHEBI_SO_EXT:DNA	denotes	DNA
T8777	28368-28377	CHEBI_EXT:polyatomic_entity_or_group	denotes	molecular
T8778	28387-28416	GO:0006270	denotes	initiation of DNA replication
T8779	28401-28404	CHEBI_SO_EXT:DNA	denotes	DNA
T8780	28442-28445	GO:0042555	denotes	Mcm
T8781	28474-28484	NCBITaxon:2759	denotes	eukaryotic
T8782	28485-28496	GO:0006260	denotes	replicative
T8783	28497-28505	GO_EXT:0004386	denotes	helicase
T8784	28562-28569	CHEMINF_GO_EXT:chemical_binding_or_bond_formation	denotes	binding
T8785	28574-28582	GO_EXT:0004386	denotes	helicase
T8786	28594-28599	NCBITaxon:10088	denotes	mouse
T8787	28600-28604	PR_EXT:000010246	denotes	Mcm4
T8788	28600-28608	GO:0097373	denotes	Mcm4/6/7
T8789	28644-28648	GO:0005657	denotes	fork
T9008	28682-28689	CHEMINF_GO_EXT:chemical_binding_or_bond_formation	denotes	binding
T9009	28708-28712	PR_EXT:000010246	denotes	Mcm4
T9010	28708-28716	GO:0097373	denotes	Mcm4/6/7
T9011	28717-28725	GO_EXT:0004386	denotes	helicase
T9012	28748-28777	GO:0006270	denotes	Initiation of DNA replication
T9013	28762-28765	CHEBI_SO_EXT:DNA	denotes	DNA
T9014	28797-28806	GO_PATO_EXT:biological_localization_process_or_quality	denotes	localized
T9015	28807-28828	GO:0032508	denotes	melting of duplex DNA
T9016	28818-28828	CHEBI_SO_EXT:double_stranded_DNA	denotes	duplex DNA
T9017	28834-28845	GO:0006260	denotes	replication
T9018	28834-28853	SO_EXT:0000296	denotes	replication origins
T9019	28855-28864	GO_EXT:0004386	denotes	Helicases
T9020	28881-28892	GO:0006260	denotes	replication
T9021	28881-28898	GO:0005657	denotes	replication forks
T9022	28911-28917	GO:0032508	denotes	melted
T9023	28947-28954	CHEMINF_GO_EXT:chemical_binding_or_bond_formation	denotes	binding
T9024	28959-28967	NCBITaxon:2	denotes	bacteria
T9025	29002-29006	PR_EXT:000010246	denotes	Mcm4
T9026	29002-29010	GO:0097373	denotes	Mcm4/6/7
T9027	29073-29076	CHEBI_SO_EXT:DNA	denotes	DNA
T9028	29073-29085	GO_EXT:0003678	denotes	DNA helicase
T9029	29093-29098	GO:0005657	denotes	forks
T9030	29120-29124	PR_EXT:000010246	denotes	Mcm4
T9031	29120-29128	GO:0097373	denotes	Mcm4/6/7
T9032	29185-29195	CHEBI_SO_EXT:double_stranded_DNA	denotes	duplex DNA
T9033	29230-29233	GO:0042555	denotes	Mcm
T9034	29260-29275	SO:0000984	denotes	single-stranded
T9035	29316-29320	PR_EXT:000010246	denotes	Mcm4
T9036	29316-29332	GO:0097373	denotes	Mcm4/6/7 complex
T9037	29364-29371	CHEBI_EXT:thymine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	thymine
T9038	29377-29386	SO_EXT:biological_sequence	denotes	sequences
T9039	29398-29413	SO:0000984	denotes	single-stranded
T9040	29426-29434	GO_EXT:0004386	denotes	helicase
T9041	29493-29501	GO_EXT:0004386	denotes	helicase
T9042	29512-29516	PR_EXT:000010246	denotes	Mcm4
T9043	29512-29520	GO:0097373	denotes	Mcm4/6/7
T9044	29575-29583	SO:0000984	denotes	unpaired
T9045	29625-29627	CHEBI_SO_EXT:nucleotide	denotes	nt
T9046	29647-29658	_FRAGMENT	denotes	assembly of
T9047	29678-29685	GO:0065003	denotes	complex
T9048	29661-29685	GO_EXT:double_hexameric_macromolecular_complex	denotes	double-hexameric complex
T9049	29703-29706	CHEBI_SO_EXT:DNA	denotes	DNA
T9050	29719-29723	NCBITaxon:10633	denotes	SV40
T9051	29726-29733	CHEBI_EXT:59132	denotes	antigen
T9052	29734-29741	CHEBI_PR_EXT:protein	denotes	protein
T9053	29783-29798	SO:0000984	denotes	single-stranded
T9054	29810-29811	CHEBI_EXT:thymine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	T
T9055	29828-29836	SO_EXT:sequence_substitution_process	denotes	replaced
T9056	29840-29847	CHEBI_EXT:guanine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	guanine
T9057	29853-29862	SO_EXT:biological_sequence	denotes	sequences
T9058	29957-29961	PR_EXT:000010246	denotes	Mcm4
T9059	29957-29965	GO:0097373	denotes	Mcm4/6/7
T9060	29987-29989	CHEBI_SO_EXT:nucleotide	denotes	nt
T9061	29990-30009	CHEBI_SO_EXT:single_stranded_DNA	denotes	single-stranded DNA
T9062	30111-30115	GO:0005657	denotes	fork
T9063	30121-30125	PR_EXT:000010246	denotes	Mcm4
T9064	30121-30137	GO:0097373	denotes	Mcm4/6/7 complex
T9065	30164-30183	CHEBI_SO_EXT:single_stranded_DNA	denotes	single-stranded DNA
T9066	30199-30207	GO_EXT:hexameric_macromolecular_complex	denotes	hexamers
T9067	30212-30216	CHEMINF_GO_EXT:chemical_binding_or_bond_formation	denotes	bind
T9068	30266-30282	GO_EXT:double_hexameric_macromolecular_complex	denotes	double hexameric
T9069	30430-30438	GO_EXT:hexameric_macromolecular_complex	denotes	hexamers
T9070	30495-30514	CHEBI_SO_EXT:single_stranded_DNA	denotes	single-stranded DNA
T9071	30582-30589	CHEMINF_GO_EXT:chemical_binding_or_bond_formation	denotes	binding
T9072	30594-30602	GO_EXT:0004386	denotes	helicase
T9073	30616-30623	CHEBI_EXT:59132	denotes	antigen
T9074	30690-30694	PR_EXT:000010246	denotes	Mcm4
T9075	30690-30698	GO:0097373	denotes	Mcm4/6/7
T9076	30699-30706	GO_EXT:hexameric_macromolecular_complex	denotes	hexamer
T9077	30715-30719	GO:0005657	denotes	fork
T9078	30780-30785	NCBITaxon:10088	denotes	mouse
T9079	30790-30796	NCBITaxon:2157	denotes	archea
T9080	30797-30800	GO:0042555	denotes	MCM
T9081	30817-30825	CHEBI:10545	denotes	electron
T9082	30899-30903	NCBITaxon:10633	denotes	SV40
T9083	30912-30919	CHEBI_EXT:59132	denotes	antigen
T9084	30951-30967	GO_EXT:double_hexameric_macromolecular_complex	denotes	double-hexameric
T9085	30981-30986	NCBITaxon:10088	denotes	mouse
T9086	30987-30990	GO:0042555	denotes	Mcm
T9087	31003-31006	CHEBI_SO_EXT:DNA	denotes	DNA
T9739	31023-31031	SO_EXT:biological_sequence	denotes	sequence
T9740	31048-31052	PR_EXT:000010246	denotes	Mcm4
T9741	31048-31056	GO:0097373	denotes	Mcm4/6/7
T9742	31057-31065	GO_EXT:0004386	denotes	helicase
T9743	31077-31080	CHEBI_SO_EXT:DNA	denotes	DNA
T9744	31081-31088	CHEMINF_GO_EXT:chemical_binding_or_bond_formation	denotes	binding
T9745	31110-31114	PR_EXT:000010246	denotes	Mcm4
T9746	31110-31118	GO:0097373	denotes	Mcm4/6/7
T9747	31119-31124	CHEMINF_GO_EXT:chemical_binding_or_bond_formation	denotes	binds
T9748	31156-31175	CHEBI_SO_EXT:single_stranded_DNA	denotes	single-stranded DNA
T9749	31225-31240	SO:0000984	denotes	single-stranded
T9750	31255-31267	_FRAGMENT	denotes	Unwinding of
T9751	31272-31282	GO:0032508	denotes	duplex DNA
T9752	31272-31282	CHEBI_SO_EXT:double_stranded_DNA	denotes	duplex DNA
T9753	31294-31307	GO_EXT:biological_translocation_entity_or_process	denotes	translocation
T9754	31311-31330	CHEBI_SO_EXT:single_stranded_DNA	denotes	single-stranded DNA
T9755	31390-31398	GO_EXT:0004386	denotes	helicase
T9756	31411-31415	GO:0005657	denotes	fork
T9757	31424-31436	GO:0006272	denotes	3′-extension
T9758	31450-31454	GO:0005657	denotes	fork
T9759	31462-31466	GO:0005657	denotes	fork
T9760	31478-31490	GO:0006273	denotes	5′-extension
T9761	31543-31562	CHEBI_SO_EXT:single_stranded_DNA	denotes	single-stranded DNA
T9762	31570-31574	PR_EXT:000010246	denotes	Mcm4
T9763	31570-31578	GO:0097373	denotes	Mcm4/6/7
T9764	31592-31601	GO:0097617	denotes	annealing
T9765	31602-31617	CHEBI_SO_EXT:oligonucleotide	denotes	oligonucleotide
T9766	31623-31631	SO:0000988	denotes	circular
T9767	31632-31651	CHEBI_SO_EXT:single_stranded_DNA	denotes	single-stranded DNA
T9768	31672-31677	NCBITaxon:10088	denotes	mouse
T9769	31678-31682	PR_EXT:000010246	denotes	Mcm4
T9770	31678-31686	GO:0097373	denotes	Mcm4/6/7
T9771	31697-31709	GO:0006272	denotes	3′-extension
T9772	31727-31735	NCBITaxon:2157	denotes	archaeal
T9773	31736-31739	GO:0042555	denotes	Mcm
T9774	31740-31748	GO_EXT:0004386	denotes	helicase
T9775	31760-31764	PR_EXT:000010246	denotes	Mcm4
T9776	31760-31768	GO:0097373	denotes	Mcm4/6/7
T9777	31774-31781	NCBITaxon:4896	denotes	S.pombe
T9778	31786-31798	NCBITaxon:4932	denotes	S.cerevisiae
T9779	31815-31823	NCBITaxon:2157	denotes	archaeal
T9780	31824-31827	GO:0042555	denotes	Mcm
T9781	31841-31845	GO:0005657	denotes	fork
T9782	31854-31864	NCBITaxon:2759	denotes	eukaryotic
T9783	31865-31869	PR_EXT:000010246	denotes	Mcm4
T9784	31865-31873	GO:0097373	denotes	Mcm4/6/7
T9785	31899-31904	CHEMINF_GO_EXT:chemical_binding_or_bond_formation	denotes	binds
T9786	31908-31927	CHEBI_SO_EXT:double_stranded_DNA	denotes	double-stranded DNA
T9787	31966-31971	NCBITaxon_EXT:yeast	denotes	yeast
T9788	31972-31975	GO:0042555	denotes	Mcm
T9789	31976-31985	GO_EXT:0004386	denotes	helicases
T9790	31990-32001	GO_EXT:biological_movement_or_translocation_process	denotes	translocate
T9791	32005-32015	CHEBI_SO_EXT:double_stranded_DNA	denotes	duplex DNA
T9792	32053-32062	NCBITaxon:40674	denotes	mammalian
T9793	32063-32067	PR_EXT:000010246	denotes	Mcm4
T9794	32063-32071	GO:0097373	denotes	Mcm4/6/7
T9795	32112-32121	SO_EXT:biological_sequence	denotes	sequences
T9796	32155-32162	CHEBI_EXT:adenine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	adenine
T9797	32167-32174	CHEBI_EXT:thymine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	thymine
T9798	32185-32196	GO:0006260	denotes	replication
T9799	32185-32204	SO_EXT:0000296	denotes	replication origins
T9800	32230-32233	GO:0042555	denotes	Mcm
T9801	32286-32295	NCBITaxon:40674	denotes	mammalian
T9802	32296-32299	CHEBI_SO_EXT:DNA	denotes	DNA
T9803	32296-32311	GO:0006260	denotes	DNA replication
T9804	32355-32364	SO_EXT:biological_sequence	denotes	sequences
T9805	32370-32375	NCBITaxon:9606	denotes	human
T9806	32376-32387	GO:0006260	denotes	replication
T9807	32376-32395	SO_EXT:0000296	denotes	replication origins
T9808	32408-32412	PR_EXT:000010246	denotes	Mcm4
T9809	32408-32425	GO:0097373	denotes	Mcm4/6/7 helicase
T9810	32417-32425	GO_EXT:0004386	denotes	helicase
T9811	32432-32440	PR_EXT:000009861	denotes	lamin-B2
T9812	32445-32450	PR_EXT:000000084	denotes	c-myc
T9813	32451-32458	SO_EXT:0000296	denotes	origins
T9814	32493-32496	GO:0042555	denotes	Mcm
T9815	32497-32505	GO_EXT:0004386	denotes	helicase
T9816	32561-32564	GO:0042555	denotes	Mcm
T9817	32572-32575	CHEBI_SO_EXT:DNA	denotes	DNA
T9818	32572-32598	GO:0006270	denotes	DNA replication initiation
T9819	32611-32616	PR_EXT:000000084	denotes	c-myc
T9820	32686-32694	SO_EXT:biological_sequence	denotes	sequence
T9821	32710-32729	CHEBI_SO_EXT:single_stranded_DNA	denotes	single-stranded DNA
T9822	32737-32745	GO_EXT:0004386	denotes	helicase
T9823	32758-32761	GO:0042555	denotes	Mcm
T9824	32789-32796	CHEBI_EXT:thymine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	thymine
T9825	32842-32850	GO_EXT:0004386	denotes	helicase
T9826	32909-32916	CHEBI_EXT:thymine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	thymine
T9827	32984-32991	CHEBI_EXT:thymine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	thymine
T9828	32992-33000	CHEBI_EXT:residue	denotes	residues
T9829	33040-33047	SO_EXT:sequence_repeat_unit_or_region	denotes	repeats
T9830	33054-33067	CHEBI_SO_EXT:dinucleotide	denotes	dinucleotides
T9831	33101-33105	PR_EXT:000010246	denotes	Mcm4
T9832	33101-33109	GO:0097373	denotes	Mcm4/6/7
T9833	33181-33200	CHEBI_SO_EXT:single_stranded_DNA	denotes	single-stranded DNA
T9834	33219-33227	GO_EXT:0004386	denotes	helicase
T9835	33236-33244	GO_EXT:0004518	denotes	Nuclease
T9836	33280-33287	CHEMINF_GO_EXT:chemical_binding_or_bond_formation	denotes	binding
T9837	33377-33381	PR_EXT:000010246	denotes	Mcm4
T9838	33377-33385	GO:0097373	denotes	Mcm4/6/7
T9839	33386-33394	GO_EXT:0004386	denotes	helicase
T9840	33443-33462	CHEBI_SO_EXT:single_stranded_DNA	denotes	single-stranded DNA
T9841	33468-33475	CHEBI_EXT:thymine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	thymine
T9842	33553-33556	CHEBI_SO_EXT:DNA	denotes	DNA
T9843	33567-33574	CHEBI_EXT:thymine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	thymine
T9844	33615-33623	SO_EXT:biological_sequence	denotes	sequence
T9845	33640-33643	GO:0042555	denotes	Mcm
T9846	33644-33652	GO_EXT:0004386	denotes	helicase
T9847	33738-33746	SO_EXT:biological_sequence	denotes	sequence
T9848	33820-33830	GO:0065007	denotes	regulation
T9849	33853-33882	GO:0006270	denotes	initiation of DNA replication
T9850	33867-33870	CHEBI_SO_EXT:DNA	denotes	DNA
T9851	33916-33920	CL_GO_EXT:cell	denotes	cell
T9852	33987-33998	GO:0006260	denotes	replication
T9853	33987-34005	SO_EXT:0000296	denotes	replication origin
T9854	34013-34019	SO_EXT:0001026	denotes	genome
T9855	34043-34047	CL_GO_EXT:cell	denotes	cell
T10709	34055-34059	PR_EXT:000010246	denotes	Mcm4
T10710	34055-34063	GO:0097373	denotes	Mcm4/6/7
T10711	34064-34072	GO_EXT:0004386	denotes	helicase
T10712	34108-34112	GO:0005657	denotes	fork
T10713	34141-34156	SO:0000984	denotes	single-stranded
T10714	34157-34164	CHEBI_EXT:thymine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	thymine
T10715	34165-34173	CHEBI_EXT:residue	denotes	residues
T10716	34192-34195	GO:0042555	denotes	Mcm
T10717	34196-34204	GO_EXT:0004386	denotes	helicase
T10718	34274-34297	GO:0032508	denotes	unwinding of duplex DNA
T10719	34287-34297	CHEBI_SO_EXT:double_stranded_DNA	denotes	duplex DNA
T10720	34341-34345	SO_EXT:0000028	denotes	pair
T10721	34353-34359	SO:0000985	denotes	duplex
T10722	34396-34399	GO:0042555	denotes	Mcm
T10723	34400-34408	GO_EXT:0004386	denotes	helicase
T10724	34419-34429	CHEBI_SO_EXT:double_stranded_DNA	denotes	Duplex DNA
T10725	34450-34455	SO_EXT:0000028	denotes	pairs
T10726	34460-34467	SO_EXT:sequence_repeat_unit_or_region	denotes	repeats
T10727	34483-34487	GO:0005657	denotes	fork
T10728	34563-34567	NCBITaxon:10633	denotes	SV40
T10729	34570-34577	CHEBI_EXT:59132	denotes	antigen
T10730	34591-34594	GO:0042555	denotes	Mcm
T10731	34595-34603	GO_EXT:0004386	denotes	helicase
T10732	34645-34651	SO:0000985	denotes	duplex
T10733	34670-34678	SO:0000988	denotes	circular
T10734	34679-34694	SO:0000984	denotes	single-stranded
T10735	34709-34715	SO:0000985	denotes	duplex
T10736	34755-34759	PR_EXT:000010246	denotes	Mcm4
T10737	34755-34763	GO:0097373	denotes	Mcm4/6/7
T10738	34785-34788	CHEBI_SO_EXT:DNA	denotes	DNA
T10739	34906-34912	SO:0000985	denotes	duplex
T10740	34923-34926	GO:0042555	denotes	Mcm
T10741	34946-34954	SO:0000988	denotes	circular
T10742	34955-34974	CHEBI_SO_EXT:single_stranded_DNA	denotes	single-stranded DNA
T10743	34983-34984	CHEBI_SO_EXT:base	denotes	b
T10744	35017-35019	CHEBI_SO_EXT:nucleotide	denotes	nt
T10745	35033-35036	CHEBI_SO_EXT:DNA	denotes	DNA
T10746	35046-35050	GO:0005657	denotes	fork
T10747	35076-35084	GO_EXT:0004386	denotes	helicase
T10748	35123-35126	GO:0042555	denotes	Mcm
T10749	35176-35184	SO_EXT:biological_sequence	denotes	sequence
T10750	35210-35216	SO:0000985	denotes	duplex
T10751	35263-35266	GO:0042555	denotes	Mcm
T10752	35267-35275	GO_EXT:0004386	denotes	helicase
T10753	35337-35343	SO:0000985	denotes	duplex
T10754	35358-35362	GO:0005657	denotes	fork
T10755	35390-35394	CHEBI_SO_EXT:base	denotes	base
T10756	35390-35400	SO_EXT:0000028	denotes	base pairs
T10757	35419-35426	SO_EXT:sequence_repeat_unit_or_region	denotes	repeats
T10758	35494-35501	CHEBI_EXT:thymine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	thymine
T10759	35502-35510	CHEBI_EXT:residue	denotes	residues
T10760	35531-35541	CHEBI_SO_EXT:double_stranded_DNA	denotes	duplex DNA
T10761	35576-35580	PR_EXT:000010246	denotes	Mcm4
T10762	35576-35584	GO:0097373	denotes	Mcm4/6/7
T10763	35640-35645	SO_EXT:0000028	denotes	pairs
T10764	35647-35658	SO_EXT:sequence_substitution_entity_or_process	denotes	Replacement
T10765	35674-35683	CHEBI_EXT:guanine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	guanosine
T10766	35689-35696	CHEBI_SO_EXT:inosine	denotes	inosine
T10767	35708-35714	SO_EXT:sequence_repeat_unit_or_region	denotes	repeat
T10768	35715-35725	CHEBI_SO_EXT:double_stranded_DNA	denotes	duplex DNA
T10769	35815-35819	CHEBI_SO_EXT:base	denotes	base
T10770	35815-35824	SO_EXT:0000028	denotes	base pair
T10771	35854-35857	GO:0042555	denotes	Mcm
T10772	35858-35866	GO_EXT:0004386	denotes	helicase
T10773	35911-35915	CHEBI_SO_EXT:base	denotes	base
T10774	35911-35921	SO_EXT:0000028	denotes	base pairs
T10775	35940-35948	GO_EXT:0004386	denotes	helicase
T10776	35959-35963	PR_EXT:000010246	denotes	Mcm4
T10777	35959-35967	GO:0097373	denotes	Mcm4/6/7
T10778	36051-36060	NCBITaxon:40674	denotes	mammalian
T10779	36061-36065	PR_EXT:000010246	denotes	Mcm4
T10780	36061-36069	GO:0097373	denotes	Mcm4/6/7
T10781	36070-36078	GO_EXT:0004386	denotes	helicase
T10782	36125-36135	NCBITaxon:2759	denotes	eukaryotic
T10783	36136-36147	GO:0006260	denotes	replicative
T10784	36148-36156	GO_EXT:0004386	denotes	helicase
T10785	36167-36196	GO:0006270	denotes	initiation of DNA replication
T10786	36181-36184	CHEBI_SO_EXT:DNA	denotes	DNA
T10787	36198-36201	GO:0042555	denotes	Mcm
T10788	36202-36210	GO_EXT:0004386	denotes	helicase
T10789	36223-36247	GO_EXT:double_hexameric_macromolecular_complex	denotes	double hexameric complex
T10790	36265-36271	GO:0032508	denotes	melted
T10791	36272-36278	SO_EXT:0000296	denotes	origin
T10792	36295-36303	GO_MOP_EXT:catalysis	denotes	catalyze
T10793	36315-36338	GO:0032508	denotes	unwinding of duplex DNA
T10794	36328-36338	CHEBI_SO_EXT:double_stranded_DNA	denotes	duplex DNA
T10795	36394-36420	SO_EXT:0000296	denotes	origins of DNA replication
T10796	36405-36408	CHEBI_SO_EXT:DNA	denotes	DNA
T10797	36405-36420	GO:0006260	denotes	DNA replication
T10798	36447-36454	CHEBI_EXT:59132	denotes	antigen
T10799	36544-36552	GO_EXT:hexameric_macromolecular_complex	denotes	hexamers
T10800	36586-36593	CHEBI_EXT:thymine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	thymine
T10801	36599-36608	SO_EXT:biological_sequence	denotes	sequences
T10802	36628-36634	GO:0032508	denotes	melted
T10803	36647-36650	GO:0042555	denotes	Mcm
T10804	36651-36659	GO_EXT:0004386	denotes	helicase
T10805	36663-36672	GO_EXT:biological_recruitment	denotes	mobilized
T10806	36733-36749	_FRAGMENT	denotes	double hexameric
T10807	36759-36768	GO_EXT:double_hexameric_macromolecular_complex	denotes	complexes
T10808	36750-36758	GO_EXT:0004386	denotes	helicase
T10809	36750-36768	GO:0033202	denotes	helicase complexes
T10810	36804-36813	GO_EXT:0004386	denotes	helicases
T10811	36821-36828	SO_EXT:0000296	denotes	origins
T10812	36857-36878	GO:0032508	denotes	melting of duplex DNA
T10813	36868-36878	CHEBI_SO_EXT:double_stranded_DNA	denotes	duplex DNA
T10814	36908-36915	CHEMINF_GO_EXT:chemical_binding_or_bond_formation	denotes	binding
T10815	36919-36924	GO:0005656	denotes	preRC
T10816	36961-36970	GO:0000785	denotes	chromatin
T10817	37025-37038	GO_EXT:0003916	denotes	topoisomerase
T10818	37061-37065	PR_EXT:000010250	denotes	Mcm8
T10819	37066-37074	GO_EXT:0004386	denotes	helicase
T10820	37119-37123	GO:0005657	denotes	fork
T10821	37134-37142	CHEBI_PR_EXT:protein	denotes	proteins
T10822	37151-37156	PR_EXT:000005215	denotes	Cdc45
T10823	37176-37191	GO_PATO_EXT:phosphorylation_process	denotes	phosphorylation
T10824	37202-37205	_FRAGMENT	denotes	Cdk
T10825	37215-37222	GO_EXT:0097472	denotes	kinases
T10826	37210-37214	PR_EXT:000005218	denotes	Cdc7
T10827	37276-37284	GO_EXT:0004386	denotes	helicase
T10828	37297-37301	PR_EXT:000010246	denotes	Mcm4
T10829	37297-37305	GO:0097373	denotes	Mcm4/6/7
T10830	37317-37327	CHEBI_SO_EXT:double_stranded_DNA	denotes	duplex DNA
T10831	37379-37383	PR_EXT:000010246	denotes	Mcm4
T10854	37845-37851	SO_EXT:0001026	denotes	genome
T1419	4632-4640	CHEBI_EXT:33893	denotes	Reagents
T1420	4641-4648	CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process	denotes	Labeled
T1421	4655-4662	CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process	denotes	labeled
T1422	4663-4668	CHEBI_EXT:dNTP	denotes	dNTPs
T1423	4669-4674	CHEBI_EXT:ribonucleoside_triphosphate	denotes	rNTPs
T1424	4749-4764	_FRAGMENT	denotes	single-stranded
T1425	4774-4777	CHEBI_SO_EXT:single_stranded_DNA	denotes	DNA
T1426	4765-4773	SO:0000988	denotes	circular
T1427	4779-4781	NCBITaxon:10665	denotes	T4
T1428	4782-4796	CHEBI:15986	denotes	polynucleotide
T1429	4782-4803	PR_EXT:000012924	denotes	polynucleotide kinase
T1430	4782-4803	GO_EXT:0051731	denotes	polynucleotide kinase
T1434	4900-4902	GO:0042571	denotes	Ab
T10832	37379-37387	GO:0097373	denotes	Mcm4/6/7
T10833	37388-37396	GO_EXT:0004386	denotes	helicase
T10834	37426-37437	GO:0006260	denotes	replicative
T10835	37438-37446	GO_EXT:0004386	denotes	helicase
T10836	37454-37465	GO:0006260	denotes	replication
T10837	37454-37471	GO:0005657	denotes	replication forks
T10838	37496-37500	PR_EXT:000010246	denotes	Mcm4
T10839	37496-37512	GO:0097373	denotes	Mcm4/6/7 complex
T10840	37549-37559	CHEBI_SO_EXT:double_stranded_DNA	denotes	duplex DNA
T10841	37556-37571	GO:0006260	denotes	DNA replication
T10842	37556-37577	GO:0005657	denotes	DNA replication forks
T10843	37579-37583	PR_EXT:000010246	denotes	Mcm4
T10844	37579-37587	GO:0097373	denotes	Mcm4/6/7
T10845	37640-37648	CHEBI_PR_EXT:protein	denotes	proteins
T10846	37670-37673	GO:0042555	denotes	Mcm
T10847	37684-37689	PR_EXT:000005215	denotes	Cdc45
T10848	37699-37703	GO:0000811	denotes	GINS
T10849	37713-37716	CHEBI_SO_EXT:DNA	denotes	DNA
T10850	37713-37727	GO_EXT:0034061	denotes	DNA polymerase
T10851	37743-37747	PR_EXT:000010250	denotes	Mcm8
T10852	37802-37810	GO_EXT:0004386	denotes	helicase
T10853	37822-37833	GO:0006260	denotes	replicating
R6454	T9751	T9750	_lexicallyChainedTo	duplex DNA,Unwinding of
R7065	T10807	T10806	_lexicallyChainedTo	complexes,double hexameric
R7066	T10825	T10824	_lexicallyChainedTo	kinases,Cdk
R2	T92	T91	_lexicallyChainedTo	of DNA replication,initiation
R3	T119	T118	_lexicallyChainedTo	DNA,single-stranded
R276	T627	T626	_lexicallyChainedTo	triphosphate,nucleotide
R277	T660	T659	_lexicallyChainedTo	7,Mcm
R871	T1425	T1424	_lexicallyChainedTo	DNA,single-stranded
R1119	T1906	T1905	_lexicallyChainedTo	DNA,single-stranded
R1120	T1917	T1916	_lexicallyChainedTo	extension,3′-
R1121	T1944	T1943	_lexicallyChainedTo	DNA,single-stranded
R1122	T1952	T1951	_lexicallyChainedTo	DNA,single-stranded
R1123	T1968	T1967	_lexicallyChainedTo	DNAs,single-stranded
R2615	T4007	T4006	_lexicallyChainedTo	complexes,Formation of
R2616	T4009	T4008	_lexicallyChainedTo	complexes,double hexameric
R3144	T4694	T4693	_lexicallyChainedTo	DNA,unwinding of
R3463	T5196	T5195	_lexicallyChainedTo	extension,3′-
R3964	T5875	T5874	_lexicallyChainedTo	yeast,fission
R3965	T5909	T5908	_lexicallyChainedTo	6,Mcm
R3966	T5910	T5908	_lexicallyChainedTo	7,Mcm
R5123	T7654	T7653	_lexicallyChainedTo	DNA,single-stranded
R5996	T9047	T9046	_lexicallyChainedTo	complex,assembly of

craft-ca-core-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id	Subject	Object	Predicate	Lexical cue
T54	36-41	NCBITaxon:10088	denotes	mouse
T55	42-46	PR:000010246	denotes	MCM4
T56	42-50	GO:0097373	denotes	MCM4/6/7
T57	102-112	_FRAGMENT	denotes	initiation
T58	128-146	GO:0006270	denotes	of DNA replication
T59	117-146	GO:0006271	denotes	elongation of DNA replication
T60	214-223	NCBITaxon:40674	denotes	mammalian
T61	224-228	PR:000010246	denotes	Mcm4
T62	224-240	GO:0097373	denotes	Mcm4/6/7 complex
T63	284-299	SO:0000984	denotes	single-stranded
T64	422-426	PR:000010246	denotes	Mcm4
T65	422-430	GO:0097373	denotes	Mcm4/6/7
T66	488-503	SO:0000984	denotes	single-stranded
T67	766-770	PR:000010246	denotes	Mcm4
T68	766-774	GO:0097373	denotes	Mcm4/6/7
T69	829-833	PR:000010246	denotes	Mcm4
T70	829-837	GO:0097373	denotes	Mcm4/6/7
T71	864-868	GO:0005657	denotes	fork
T72	905-920	SO:0000984	denotes	single-stranded
T73	1091-1110	GO:0000732	denotes	Strand displacement
T74	1114-1118	PR:000010246	denotes	Mcm4
T75	1114-1122	GO:0097373	denotes	Mcm4/6/7
T76	1162-1168	SO:0000985	denotes	duplex
T77	1303-1312	NCBITaxon:40674	denotes	mammalian
T78	1313-1317	PR:000010246	denotes	Mcm4
T79	1313-1321	GO:0097373	denotes	Mcm4/6/7
T80	1368-1379	SO:0000028	denotes	base-paired
T81	1491-1501	NCBITaxon:2759	denotes	eukaryotic
T82	1502-1513	GO:0006260	denotes	replicative
T547	1563-1573	MOP:0000619	denotes	hydrolysis
T548	1623-1639	GO:0032508	denotes	duplex unwinding
T549	1734-1744	MOP:0000619	denotes	hydrolysis
T550	1761-1784	GO:0032508	denotes	unwinding of duplex DNA
T551	1774-1780	SO:0000985	denotes	duplex
T552	1826-1852	GO:0042555	denotes	minichromosome maintenance
T553	1854-1857	GO:0042555	denotes	Mcm
T554	1983-1998	NCBITaxon:10633	denotes	simian virus 40
T555	2007-2014	CHEBI:59132	denotes	antigen
T556	2020-2036	NCBITaxon:562	denotes	Escherichia coli
T557	2037-2041	PR:000022460	denotes	DnaB
T558	2050-2058	NCBITaxon:2157	denotes	Archaeal
T559	2117-2127	NCBITaxon:2759	denotes	eukaryotic
T560	2128-2131	GO:0042555	denotes	Mcm
T561	2171-2175	PR:000010246	denotes	Mcm4
T562	2171-2179	GO:0097373	denotes	Mcm4/6/7
T563	2210-2213	GO:0042555	denotes	Mcm
T564	2210-2215	PR:000010242	denotes	Mcm 2
T565	2210-2213	_FRAGMENT	denotes	Mcm
T566	2216-2217	PR:000010249	denotes	7
T567	2342-2351	GO:0032991	denotes	complexes
T568	2358-2361	GO:0042555	denotes	MCM
T569	2394-2398	PR:000010246	denotes	Mcm4
T570	2394-2410	GO:0097373	denotes	Mcm4/6/7 complex
T571	2493-2497	PR:000010246	denotes	Mcm4
T572	2499-2503	PR:000010248	denotes	Mcm6
T573	2508-2512	PR:000010249	denotes	Mcm7
T574	2583-2587	PR:000010242	denotes	Mcm2
T575	2591-2595	PR:000010243	denotes	Mcm3
T576	2635-2639	PR:000010246	denotes	Mcm4
T577	2635-2651	GO:0097373	denotes	Mcm4/6/7 complex
T578	2756-2765	GO:0000785	denotes	Chromatin
T579	2797-2804	SO:0000704	denotes	genetic
T580	2825-2849	NCBITaxon:4932	denotes	Saccharomyces cerevisiae
T581	2874-2877	GO:0042555	denotes	Mcm
T582	2929-2949	GO:0022616	denotes	DNA chain elongation
T583	2961-2972	GO:0006260	denotes	replicative
T584	3044-3069	NCBITaxon:4896	denotes	Schizosaccharomyces pombe
T585	3074-3079	NCBITaxon:10088	denotes	mouse
T586	3080-3084	PR:000010246	denotes	Mcm4
T587	3080-3098	GO:0097373	denotes	Mcm4/6/7 complexes
T588	3130-3136	GO:0005657	denotes	forked
T589	3170-3176	SO:0000985	denotes	duplex
T590	3192-3194	SO:0000028	denotes	bp
T591	3204-3208	PR:000010246	denotes	Mcm4
T592	3204-3212	GO:0097373	denotes	Mcm4/6/7
T593	3222-3226	GO:0005657	denotes	fork
T594	3338-3345	CHEBI:59132	denotes	antigen
T595	3406-3416	MOP:0000619	denotes	hydrolysis
T596	3431-3440	NCBITaxon:40674	denotes	mammalian
T597	3441-3445	PR:000010246	denotes	Mcm4
T598	3441-3449	GO:0097373	denotes	Mcm4/6/7
T599	3480-3495	SO:0000984	denotes	single-stranded
T600	3573-3576	GO:0042555	denotes	Mcm
T601	3617-3628	GO:0006260	denotes	replication
T602	3617-3636	SO:0000296	denotes	replication origins
T603	3647-3657	NCBITaxon:2759	denotes	eukaryotes
T604	3726-3729	GO:0042555	denotes	Mcm
T605	3790-3801	GO:0006260	denotes	replication
T606	3790-3807	GO:0005657	denotes	replication forks
T607	3891-3896	NCBITaxon:10088	denotes	mouse
T608	3897-3901	PR:000010246	denotes	Mcm4
T609	3897-3905	GO:0097373	denotes	Mcm4/6/7
T610	3929-3935	GO:0005657	denotes	forked
T611	4018-4021	GO:0042555	denotes	Mcm
T612	4159-4162	GO:0042555	denotes	Mcm
T613	4228-4237	NCBITaxon:40674	denotes	mammalian
T614	4238-4242	PR:000010246	denotes	Mcm4
T615	4238-4246	GO:0097373	denotes	Mcm4/6/7
T616	4266-4281	SO:0000984	denotes	single-stranded
T617	4378-4393	SO:0000984	denotes	single-stranded
T618	4417-4421	PR:000010246	denotes	Mcm4
T619	4417-4425	GO:0097373	denotes	Mcm4/6/7
T620	4487-4493	SO:0000985	denotes	duplex
T621	4574-4585	GO:0006260	denotes	replication
T622	4600-4606	SO:0001026	denotes	genome
T1415	4963-4970	CHEBI:59132	denotes	antigen
T1416	4989-5000	NCBITaxon:10442	denotes	baculovirus
T1417	5010-5016	NCBITaxon:6960	denotes	insect
T1418	5041-5045	PR:000023591	denotes	PriA
T1854	6068-6074	SO:0000985	denotes	duplex
T1855	6106-6115	GO:0097617	denotes	annealing
T1856	6154-6169	SO:0000984	denotes	single-stranded
T1857	6170-6178	SO:0000988	denotes	circular
T1858	6459-6463	GO:0005657	denotes	fork
T1859	6492-6501	GO:0097617	denotes	annealing
T1860	6573-6577	GO:0005657	denotes	fork
T1861	6582-6585	_FRAGMENT	denotes	3′-
T1862	6592-6601	GO:0006272	denotes	extension
T1863	6589-6601	GO:0006273	denotes	5′-extension
T1864	6677-6685	GO:0097617	denotes	annealed
T1865	6694-6702	PR:000009861	denotes	lamin B2
T1866	6739-6747	PR:000009861	denotes	lamin B2
T1867	6748-6754	SO:0000296	denotes	origin
T1868	6830-6835	PR:000000084	denotes	c-myc
T1869	6843-6848	PR:000000084	denotes	c-myc
T1870	7015-7021	GO:0032508	denotes	melted
T1871	7087-7095	GO:0097617	denotes	annealed
T1872	7154-7161	CHEBI:60004	denotes	mixture
T1873	7187-7191	CHEBI:9754	denotes	Tris
T1874	7192-7195	CHEBI:17883	denotes	HCl
T1875	7212-7217	CHEBI:6636	denotes	MgCl2
T1876	7228-7232	CHEBI:26710	denotes	NaCl
T1877	7442-7448	SO:0000985	denotes	duplex
T1878	7465-7480	SO:0000984	denotes	single-stranded
T1879	7481-7489	SO:0000988	denotes	circular
T1880	7517-7523	SO:0000985	denotes	duplex
T1881	7618-7629	GO:0097617	denotes	hybridizing
T1882	7664-7670	SO:0000440	denotes	vector
T1883	7672-7680	GO:0097617	denotes	annealed
T1884	7688-7703	SO:0000984	denotes	single-stranded
T1885	7704-7712	SO:0000988	denotes	circular
T1886	7781-7784	CHEBI:37972	denotes	32P
T1887	7900-7906	SO:0000985	denotes	duplex
T1888	8011-8015	PR:000010246	denotes	Mcm4
T1889	8011-8027	GO:0097373	denotes	Mcm4/6/7 complex
T1890	8056-8063	CHEBI:60004	denotes	mixture
T1891	8144-8147	CHEBI:8984	denotes	SDS
T1892	8306-8321	SO:0000984	denotes	single-stranded
T1893	8322-8330	SO:0000988	denotes	circular
T1894	8467-8476	GO:0097617	denotes	annealing
T1895	8564-8569	PR:P23940	denotes	BamHI
T1896	8611-8617	SO:0000667	denotes	insert
T2587	8690-8694	PR:000010246	denotes	Mcm4
T2588	8690-8698	GO:0097373	denotes	Mcm4/6/7
T2589	8754-8762	CHEBI:60004	denotes	mixtures
T2590	8788-8793	CHEBI:46756	denotes	HEPES
T2591	8794-8798	CHEBI:32145	denotes	NaOH
T2592	8815-8829	CHEBI:32954	denotes	sodium acetate
T2593	8837-8848	CHEBI:62964	denotes	Mg(CH3COO)2
T2594	8855-8858	CHEBI:18320	denotes	DTT
T2595	8883-8890	CHEBI:27575	denotes	ATP-γ-S
T2596	8969-8977	CHEBI:17754	denotes	glycerol
T2597	8992-9000	CHEBI:60004	denotes	mixtures
T2598	9063-9080	CHEBI:62964	denotes	magnesium acetate
T2599	9088-9096	CHEBI:17754	denotes	glycerol
T2600	9203-9211	CHEBI:60004	denotes	mixtures
T2601	9442-9445	CHEBI:8984	denotes	SDS
T2901	9652-9660	CHEBI:60004	denotes	mixtures
T2902	9685-9690	CHEBI:46756	denotes	HEPES
T2903	9691-9695	CHEBI:32145	denotes	NaOH
T2904	9712-9726	CHEBI:32954	denotes	sodium acetate
T2905	9734-9745	CHEBI:62964	denotes	Mg(CH3COO)2
T2906	9752-9755	CHEBI:18320	denotes	DTT
T2907	9780-9787	CHEBI:27575	denotes	ATP-γ-S
T2908	9796-9801	CHEBI:3312	denotes	CaCl2
T2909	9814-9817	CHEBI:37972	denotes	32P
T2910	9872-9876	PR:000010246	denotes	Mcm4
T2911	9872-9880	GO:0097373	denotes	Mcm4/6/7
T2912	10006-10014	CHEBI:60004	denotes	mixtures
T2913	10119-10127	CHEBI:75958	denotes	solution
T2914	10146-10161	CHEBI:26710	denotes	sodium chloride
T2915	10166-10169	CHEBI:8984	denotes	SDS
T2916	10306-10311	CHEBI:3312	denotes	CaCl2
T2917	10410-10418	MOP:0000780	denotes	Cleavage
T2918	10476-10480	CHEBI:32145	denotes	NaOH
T2919	10486-10493	CHEBI:60004	denotes	mixture
T2920	10580-10594	CHEBI:32954	denotes	sodium acetate
T2921	10630-10636	CHEBI:15882	denotes	phenol
T2922	10637-10647	CHEBI:35255	denotes	chloroform
T2923	10687-10694	CHEBI:16236	denotes	ethanol
T2924	10735-10742	CHEBI:16236	denotes	ethanol
T2925	10859-10863	CHEBI:16199	denotes	urea
T3303	10904-10908	PR:000010246	denotes	Mcm4
T3304	10904-10920	GO:0097373	denotes	Mcm4/6/7 complex
T3305	10950-10965	SO:0000984	denotes	single-stranded
T3306	10978-10982	PR:000010246	denotes	Mcm4
T3307	10978-10986	GO:0097373	denotes	Mcm4/6/7
T3308	11036-11042	SO:0000985	denotes	duplex
T3309	11069-11084	SO:0000984	denotes	single-stranded
T3310	11169-11173	PR:000010246	denotes	Mcm4
T3311	11169-11177	GO:0097373	denotes	Mcm4/6/7
T3312	11274-11278	PR:000010246	denotes	Mcm4
T3313	11274-11282	GO:0097373	denotes	Mcm4/6/7
T3314	11302-11309	CHEBI:27575	denotes	ATP-γ-S
T3315	11432-11441	MOP:0000780	denotes	cleavages
T3316	11466-11468	SO:0000028	denotes	bp
T3317	11469-11475	SO:0000985	denotes	duplex
T3318	11531-11540	MOP:0000780	denotes	cleavages
T3319	11570-11585	SO:0000984	denotes	single-stranded
T3320	11635-11650	SO:0000985	denotes	double-stranded
T3321	11734-11749	SO:0000984	denotes	single-stranded
T3322	11822-11826	PR:000010246	denotes	Mcm4
T3323	11822-11830	GO:0097373	denotes	Mcm4/6/7
T3324	11865-11869	PR:000010246	denotes	Mcm4
T3325	11865-11873	GO:0097373	denotes	Mcm4/6/7
T3326	11975-11990	SO:0000984	denotes	single-stranded
T3327	12055-12070	SO:0000984	denotes	single-stranded
T3328	12106-12117	GO:0042555	denotes	Mcm complex
T3329	12183-12198	SO:0000984	denotes	single-stranded
T3330	12230-12234	PR:000010246	denotes	Mcm4
T3331	12230-12238	GO:0097373	denotes	Mcm4/6/7
T3332	12395-12404	SO:0000028	denotes	base-pair
T3333	12405-12411	SO:0000985	denotes	duplex
T3334	12572-12576	PR:000010246	denotes	Mcm4
T3335	12572-12580	GO:0097373	denotes	Mcm4/6/7
T3336	12595-12606	GO:0006260	denotes	replication
T3337	12725-12729	NCBITaxon:10633	denotes	SV40
T3338	12732-12739	CHEBI:59132	denotes	antigen
T3339	12809-12813	GO:0005657	denotes	fork
T3340	12850-12865	SO:0000984	denotes	single-stranded
T3341	12893-12895	SO:0000028	denotes	bp
T3342	12896-12902	SO:0000985	denotes	duplex
T3343	12923-12929	SO:0000985	denotes	duplex
T3344	12933-12946	SO:0000984	denotes	single-strand
T3345	12974-12977	GO:0042555	denotes	Mcm
T3346	13037-13041	PR:000010246	denotes	Mcm4
T3347	13037-13045	GO:0097373	denotes	Mcm4/6/7
T3348	13079-13094	SO:0000984	denotes	single-stranded
T3349	13146-13152	SO:0000985	denotes	duplex
T3350	13190-13201	GO:0006260	denotes	replication
T3351	13190-13206	GO:0005657	denotes	replication fork
T3970	13209-13221	_FRAGMENT	denotes	Formation of
T3971	13248-13257	GO:0065003	denotes	complexes
T3972	13239-13243	PR:000010246	denotes	Mcm4
T3973	13239-13247	GO:0097373	denotes	Mcm4/6/7
T3974	13339-13343	PR:000010246	denotes	Mcm4
T3975	13339-13347	GO:0097373	denotes	Mcm4/6/7
T3976	13473-13488	SO:0000984	denotes	single-stranded
T3977	13521-13536	SO:0000984	denotes	single-stranded
T3978	13608-13619	GO:0042555	denotes	Mcm complex
T3979	13660-13664	PR:000010246	denotes	Mcm4
T3980	13660-13668	GO:0097373	denotes	Mcm4/6/7
T3981	13766-13781	SO:0000984	denotes	single-stranded
T3982	14055-14063	SO:0000984	denotes	unpaired
T3983	14133-14137	PR:000010246	denotes	Mcm4
T3984	14133-14141	GO:0097373	denotes	Mcm4/6/7
T3985	14177-14181	PR:000010246	denotes	Mcm4
T3986	14177-14185	GO:0097373	denotes	Mcm4/6/7
T3987	14273-14280	CHEBI:27575	denotes	ATP-γ-S
T3988	14290-14297	GO:0032991	denotes	complex
T3989	14290-14307	GO:0065003	denotes	complex formation
T3990	14334-14342	CHEBI:60004	denotes	mixtures
T3991	14558-14567	GO:0032991	denotes	complexes
T3992	14573-14577	PR:000010246	denotes	Mcm4
T3993	14573-14581	GO:0097373	denotes	Mcm4/6/7
T3994	14684-14688	PR:000010246	denotes	Mcm4
T3995	14684-14692	GO:0097373	denotes	Mcm4/6/7
T3996	14727-14736	GO:0032991	denotes	complexes
T3997	15018-15033	SO:0000984	denotes	single-stranded
T3998	15123-15126	GO:0042555	denotes	Mcm
T3999	15183-15187	PR:000010246	denotes	Mcm4
T4000	15183-15199	GO:0097373	denotes	Mcm4/6/7 complex
T4001	15339-15354	SO:0000984	denotes	single-stranded
T4002	15486-15501	SO:0000984	denotes	single-stranded
T4003	15684-15688	PR:000010246	denotes	Mcm4
T4004	15684-15700	GO:0097373	denotes	Mcm4/6/7 complex
T4005	15773-15779	SO:0000985	denotes	duplex
T4671	15839-15854	SO:0000984	denotes	single-stranded
T4672	15891-15903	_FRAGMENT	denotes	unwinding of
T4673	15911-15914	GO:0032508	denotes	DNA
T4674	15918-15922	PR:000010246	denotes	Mcm4
T4675	15918-15926	GO:0097373	denotes	Mcm4/6/7
T4676	15952-15956	PR:000010246	denotes	Mcm4
T4677	15952-15960	GO:0097373	denotes	Mcm4/6/7
T4678	16051-16057	SO:0000985	denotes	duplex
T4679	16227-16231	PR:000010246	denotes	Mcm4
T4680	16227-16235	GO:0097373	denotes	Mcm4/6/7
T4681	16286-16301	SO:0000984	denotes	single-stranded
T4682	16533-16537	PR:000010246	denotes	Mcm4
T4683	16533-16541	GO:0097373	denotes	Mcm4/6/7
T4684	16638-16642	PR:000010246	denotes	Mcm4
T4685	16638-16646	GO:0097373	denotes	Mcm4/6/7
T4686	16944-16950	SO:0000985	denotes	duplex
T4687	17001-17005	GO:0005657	denotes	fork
T4688	17282-17291	CHEBI:36357	denotes	molecules
T4689	17299-17303	PR:000010246	denotes	Mcm4
T4690	17299-17307	GO:0097373	denotes	Mcm4/6/7
T5136	17380-17384	PR:000010246	denotes	Mcm4
T5137	17380-17388	GO:0097373	denotes	Mcm4/6/7
T5138	17392-17398	GO:0005657	denotes	forked
T5139	17403-17406	_FRAGMENT	denotes	3′-
T5140	17413-17422	GO:0006272	denotes	extension
T5141	17410-17422	GO:0006273	denotes	5′-extension
T5142	17511-17517	GO:0005657	denotes	forked
T5143	17532-17536	GO:0005657	denotes	fork
T5144	17587-17591	GO:0005657	denotes	fork
T5145	17624-17628	GO:0005657	denotes	fork
T5146	17654-17658	GO:0005657	denotes	fork
T5147	17679-17691	GO:0006273	denotes	5′-extension
T5148	17695-17707	GO:0006272	denotes	3′-extension
T5149	17737-17741	PR:000010246	denotes	Mcm4
T5150	17737-17745	GO:0097373	denotes	Mcm4/6/7
T5151	17757-17761	GO:0005657	denotes	fork
T5152	17771-17775	GO:0005657	denotes	fork
T5153	17824-17828	GO:0005657	denotes	fork
T5154	17874-17878	PR:000023591	denotes	PriA
T5155	17919-17923	GO:0005657	denotes	fork
T5156	17955-17959	GO:0005657	denotes	fork
T5157	17997-18001	PR:000010246	denotes	Mcm4
T5158	17997-18005	GO:0097373	denotes	Mcm4/6/7
T5159	18020-18032	GO:0006273	denotes	5′-extension
T5160	18037-18049	GO:0006272	denotes	3′-extension
T5161	18103-18107	GO:0005657	denotes	fork
T5162	18118-18122	GO:0005657	denotes	fork
T5163	18140-18144	PR:000010246	denotes	Mcm4
T5164	18140-18148	GO:0097373	denotes	Mcm4/6/7
T5165	18160-18164	GO:0005657	denotes	fork
T5166	18206-18213	GO:0032991	denotes	complex
T5167	18275-18279	PR:000010246	denotes	Mcm4
T5168	18275-18291	GO:0097373	denotes	Mcm4/6/7 complex
T5169	18350-18365	SO:0000984	denotes	single-stranded
T5170	18472-18487	SO:0000984	denotes	single-stranded
T5171	18500-18504	GO:0005657	denotes	fork
T5172	18540-18544	GO:0005657	denotes	fork
T5173	18554-18558	GO:0005657	denotes	fork
T5174	18586-18590	PR:000010246	denotes	Mcm4
T5175	18586-18594	GO:0097373	denotes	Mcm4/6/7
T5176	18641-18645	GO:0005657	denotes	fork
T5177	18670-18676	SO:0000985	denotes	duplex
T5178	18703-18718	SO:0000985	denotes	double-stranded
T5179	18770-18774	PR:000010246	denotes	Mcm4
T5180	18770-18778	GO:0097373	denotes	Mcm4/6/7
T5181	18802-18806	PR:000023591	denotes	PriA
T5182	18870-18874	GO:0005657	denotes	fork
T5183	18900-18904	GO:0005657	denotes	fork
T5184	18945-18949	GO:0005657	denotes	fork
T5185	18998-19002	GO:0005657	denotes	fork
T5186	19068-19072	PR:000010246	denotes	Mcm4
T5187	19068-19084	GO:0097373	denotes	Mcm4/6/7 complex
T5188	19094-19109	SO:0000984	denotes	single-stranded
T5189	19175-19190	SO:0000984	denotes	single-stranded
T5827	19201-19213	GO:0006272	denotes	3′-extension
T5828	19249-19253	PR:000010246	denotes	Mcm4
T5829	19249-19257	GO:0097373	denotes	Mcm4/6/7
T5830	19288-19300	GO:0006272	denotes	3′-extension
T5831	19323-19331	NCBITaxon:2157	denotes	archaeal
T5832	19332-19335	GO:0042555	denotes	Mcm
T5833	19359-19366	_FRAGMENT	denotes	fission
T5834	19379-19384	NCBITaxon:4894	denotes	yeast
T5835	19371-19378	GO:0007114	denotes	budding
T5836	19371-19384	NCBITaxon:4892	denotes	budding yeast
T5837	19385-19398	GO:0042555	denotes	Mcm complexes
T5838	19431-19443	GO:0006272	denotes	3′-extension
T5839	19545-19550	NCBITaxon:10088	denotes	mouse
T5840	19551-19555	PR:000010246	denotes	Mcm4
T5841	19551-19559	GO:0097373	denotes	Mcm4/6/7
T5842	19611-19615	PR:000010246	denotes	Mcm4
T5843	19611-19619	GO:0097373	denotes	Mcm4/6/7
T5844	19645-19657	GO:0006272	denotes	3′-extension
T5845	19691-19703	GO:0006272	denotes	3′-extension
T5846	19792-19796	PR:000010246	denotes	Mcm4
T5847	19792-19800	GO:0097373	denotes	Mcm4/6/7
T5848	19897-19909	GO:0006272	denotes	3′-extension
T5849	19939-19944	PR:000010246	denotes	Mcm 4
T5850	19939-19942	_FRAGMENT	denotes	Mcm
T5851	19945-19946	PR:000010248	denotes	6
T5852	19947-19948	PR:000010249	denotes	7
T5853	19939-19948	GO:0097373	denotes	Mcm 4/6/7
T5854	20076-20080	PR:000010246	denotes	Mcm4
T5855	20076-20084	GO:0097373	denotes	Mcm4/6/7
T5856	20130-20142	GO:0006272	denotes	3′-extension
T5857	20178-20190	GO:0006273	denotes	5′-extension
T5858	20201-20205	PR:000010246	denotes	Mcm4
T5859	20201-20209	GO:0097373	denotes	Mcm4/6/7
T5860	20247-20250	GO:0042555	denotes	Mcm
T5861	20302-20314	GO:0006272	denotes	3′-extension
T5862	20404-20408	GO:0005657	denotes	fork
T5863	20515-20524	NCBITaxon:40674	denotes	mammalian
T5864	20525-20528	GO:0042555	denotes	Mcm
T5865	20594-20609	SO:0000984	denotes	single-stranded
T6337	20670-20673	GO:0042555	denotes	Mcm
T6338	20806-20809	GO:0042555	denotes	Mcm
T6339	20845-20851	SO:0000985	denotes	duplex
T6340	20856-20871	SO:0000984	denotes	single-stranded
T6341	20892-20894	SO:0000028	denotes	bp
T6342	20895-20901	SO:0000985	denotes	duplex
T6343	21035-21040	NCBITaxon:10088	denotes	mouse
T6344	21041-21045	PR:000010246	denotes	Mcm4
T6345	21041-21049	GO:0097373	denotes	Mcm4/6/7
T6346	21105-21109	PR:000010246	denotes	Mcm4
T6347	21105-21113	GO:0097373	denotes	Mcm4/6/7
T6348	21135-21143	GO:0097617	denotes	annealed
T6349	21633-21636	GO:0042555	denotes	Mcm
T6350	21696-21702	GO:0097617	denotes	anneal
T6351	21741-21744	GO:0042555	denotes	Mcm
T6352	21962-21968	SO:0000985	denotes	duplex
T6353	22069-22073	PR:000010246	denotes	Mcm4
T6354	22069-22085	GO:0097373	denotes	Mcm4/6/7 complex
T6355	22208-22211	GO:0042555	denotes	Mcm
T6356	22591-22594	GO:0042555	denotes	Mcm
T6898	22665-22670	PR:000000084	denotes	c-myc
T6899	22671-22677	SO:0000296	denotes	origin
T6900	22687-22690	GO:0042555	denotes	Mcm
T6901	22801-22806	NCBITaxon:9606	denotes	human
T6902	22807-22815	PR:000009861	denotes	lamin B2
T6903	22816-22822	SO:0000296	denotes	origin
T6904	22889-22892	GO:0042555	denotes	Mcm
T6905	23034-23045	GO:0006260	denotes	replication
T6906	23034-23053	SO:0000296	denotes	replication origins
T6907	23114-23117	GO:0042555	denotes	Mcm
T6908	23194-23199	PR:000000084	denotes	c-myc
T6909	23244-23257	GO:0032508	denotes	DNA unwinding
T6910	23281-23286	NCBITaxon:9606	denotes	human
T6911	23287-23292	PR:000000084	denotes	c-myc
T6912	23293-23299	SO:0000296	denotes	origin
T6913	23323-23328	PR:000000084	denotes	c-myc
T6914	23362-23370	SO:0000984	denotes	unpaired
T6915	23388-23393	PR:000000084	denotes	c-myc
T6916	23443-23448	PR:000000084	denotes	c-myc
T6917	23554-23559	PR:000000084	denotes	c-myc
T6918	23560-23566	SO:0000296	denotes	origin
T6919	23642-23646	PR:000010246	denotes	Mcm4
T6920	23642-23650	GO:0097373	denotes	Mcm4/6/7
T6921	23665-23673	PR:000009861	denotes	lamin B2
T6922	23695-23700	PR:000000084	denotes	c-myc
T6923	23721-23726	PR:000000084	denotes	c-myc
T6924	23815-23820	PR:000000084	denotes	c-myc
T6925	23821-23827	SO:0000296	denotes	origin
T6926	23874-23877	GO:0042555	denotes	Mcm
T6927	23968-23972	PR:000010246	denotes	Mcm4
T6928	23968-23976	GO:0097373	denotes	Mcm4/6/7
T6929	24129-24132	GO:0042555	denotes	Mcm
T6930	24175-24179	PR:000010246	denotes	MCM4
T6931	24175-24183	GO:0097373	denotes	MCM4/6/7
T6932	24247-24252	PR:000000084	denotes	c-myc
T6933	24270-24275	PR:000000084	denotes	c-myc
T6934	24320-24328	PR:000009861	denotes	lamin B2
T6935	24531-24534	GO:0042555	denotes	Mcm
T7529	24575-24588	GO:0032508	denotes	DNA unwinding
T7530	24592-24596	PR:000010246	denotes	Mcm4
T7531	24592-24600	GO:0097373	denotes	Mcm4/6/7
T7532	24646-24652	SO:0000985	denotes	duplex
T7533	24700-24715	SO:0000984	denotes	single-stranded
T7534	24774-24777	GO:0042555	denotes	Mcm
T7535	24867-24890	GO:0032508	denotes	unwinding of duplex DNA
T7536	24880-24886	SO:0000985	denotes	duplex
T7537	24962-24968	SO:0000985	denotes	duplex
T7538	25069-25073	GO:0005657	denotes	fork
T7539	25088-25092	GO:0005657	denotes	fork
T7540	25130-25136	SO:0000985	denotes	duplex
T7541	25240-25244	PR:000010246	denotes	Mcm4
T7542	25240-25248	GO:0097373	denotes	Mcm4/6/7
T7543	25266-25270	GO:0005657	denotes	fork
T7544	25355-25359	PR:000010246	denotes	Mcm4
T7545	25355-25363	GO:0097373	denotes	Mcm4/6/7
T7546	25373-25388	SO:0000984	denotes	single-stranded
T7547	25431-25435	GO:0005657	denotes	fork
T7548	25468-25472	PR:000010246	denotes	Mcm4
T7549	25468-25476	GO:0097373	denotes	Mcm4/6/7
T7550	25507-25511	NCBITaxon:10633	denotes	SV40
T7551	25514-25521	CHEBI:59132	denotes	antigen
T7552	25672-25676	GO:0005657	denotes	fork
T7553	25695-25699	GO:0005657	denotes	fork
T7554	25800-25806	SO:0000985	denotes	duplex
T7555	25818-25822	GO:0005657	denotes	fork
T7556	25840-25844	GO:0005657	denotes	fork
T7557	25862-25866	GO:0005657	denotes	fork
T7558	25887-25891	GO:0005657	denotes	fork
T7559	25939-25945	SO:0000985	denotes	duplex
T7560	26082-26088	SO:0000985	denotes	duplex
T7561	26098-26113	SO:0000984	denotes	single-stranded
T7562	26114-26122	SO:0000988	denotes	circular
T7563	26184-26190	SO:0000985	denotes	duplex
T7564	26217-26223	SO:0000985	denotes	duplex
T7565	26268-26274	SO:0000440	denotes	vector
T7566	26357-26368	GO:0097617	denotes	hybridizing
T7567	26404-26408	PR:000010246	denotes	Mcm4
T7568	26404-26412	GO:0097373	denotes	Mcm4/6/7
T7569	26435-26441	SO:0000985	denotes	duplex
T7570	26786-26790	PR:000010246	denotes	Mcm4
T7571	26786-26802	GO:0097373	denotes	Mcm4/6/7 complex
T7572	26845-26849	GO:0005657	denotes	fork
T7573	26877-26883	SO:0000985	denotes	duplex
T7574	26927-26930	GO:0042555	denotes	Mcm
T7575	27005-27010	SO:0000028	denotes	pairs
T7576	27043-27049	SO:0000985	denotes	duplex
T7577	27096-27100	PR:000010246	denotes	Mcm4
T7578	27096-27104	GO:0097373	denotes	Mcm4/6/7
T7579	27121-27125	PR:000010246	denotes	Mcm4
T7580	27121-27129	GO:0097373	denotes	Mcm4/6/7
T7581	27194-27205	SO:0000028	denotes	base-paired
T7582	27284-27288	GO:0005657	denotes	fork
T7583	27393-27402	SO:0000028	denotes	base pair
T7584	27433-27442	SO:0000028	denotes	base pair
T7585	27446-27452	SO:0000985	denotes	duplex
T7586	27517-27526	SO:0000028	denotes	base pair
T7587	27561-27565	GO:0005657	denotes	fork
T7588	27632-27638	SO:0000985	denotes	duplex
T7589	27664-27668	PR:000010246	denotes	Mcm4
T7590	27664-27672	GO:0097373	denotes	Mcm4/6/7
T7591	27746-27750	GO:0005657	denotes	fork
T7592	27812-27816	GO:0005657	denotes	fork
T7593	27887-27891	GO:0005657	denotes	fork
T7594	27948-27954	SO:0000985	denotes	duplex
T7595	27983-27992	SO:0000028	denotes	base pair
T7596	28026-28030	PR:000010246	denotes	Mcm4
T7597	28026-28034	GO:0097373	denotes	Mcm4/6/7
T8958	28708-28712	PR:000010246	denotes	Mcm4
T8959	28708-28716	GO:0097373	denotes	Mcm4/6/7
T8960	28748-28777	GO:0006270	denotes	Initiation of DNA replication
T8961	28807-28828	GO:0032508	denotes	melting of duplex DNA
T8962	28818-28824	SO:0000985	denotes	duplex
T8963	28834-28845	GO:0006260	denotes	replication
T8964	28834-28853	SO:0000296	denotes	replication origins
T8965	28881-28892	GO:0006260	denotes	replication
T8966	28881-28898	GO:0005657	denotes	replication forks
T8967	28911-28917	GO:0032508	denotes	melted
T8968	28959-28967	NCBITaxon:2	denotes	bacteria
T8969	29002-29006	PR:000010246	denotes	Mcm4
T8970	29002-29010	GO:0097373	denotes	Mcm4/6/7
T8971	29093-29098	GO:0005657	denotes	forks
T8972	29120-29124	PR:000010246	denotes	Mcm4
T8973	29120-29128	GO:0097373	denotes	Mcm4/6/7
T8974	29185-29191	SO:0000985	denotes	duplex
T8975	29230-29233	GO:0042555	denotes	Mcm
T8976	29260-29275	SO:0000984	denotes	single-stranded
T8977	29316-29320	PR:000010246	denotes	Mcm4
T8978	29316-29332	GO:0097373	denotes	Mcm4/6/7 complex
T8979	29398-29413	SO:0000984	denotes	single-stranded
T8980	29512-29516	PR:000010246	denotes	Mcm4
T8981	29512-29520	GO:0097373	denotes	Mcm4/6/7
T8982	29575-29583	SO:0000984	denotes	unpaired
T8983	29647-29658	_FRAGMENT	denotes	assembly of
T8984	29678-29685	GO:0065003	denotes	complex
T8985	29719-29723	NCBITaxon:10633	denotes	SV40
T8986	29726-29733	CHEBI:59132	denotes	antigen
T8987	29783-29798	SO:0000984	denotes	single-stranded
T8988	29957-29961	PR:000010246	denotes	Mcm4
T8989	29957-29965	GO:0097373	denotes	Mcm4/6/7
T8990	29990-30005	SO:0000984	denotes	single-stranded
T8991	30111-30115	GO:0005657	denotes	fork
T8992	30121-30125	PR:000010246	denotes	Mcm4
T8993	30121-30137	GO:0097373	denotes	Mcm4/6/7 complex
T8994	30164-30179	SO:0000984	denotes	single-stranded
T8995	30495-30510	SO:0000984	denotes	single-stranded
T8996	30616-30623	CHEBI:59132	denotes	antigen
T8997	30690-30694	PR:000010246	denotes	Mcm4
T8998	30690-30698	GO:0097373	denotes	Mcm4/6/7
T8999	30715-30719	GO:0005657	denotes	fork
T9000	30780-30785	NCBITaxon:10088	denotes	mouse
T9001	30790-30796	NCBITaxon:2157	denotes	archea
T9002	30797-30800	GO:0042555	denotes	MCM
T9003	30817-30825	CHEBI:10545	denotes	electron
T9004	30899-30903	NCBITaxon:10633	denotes	SV40
T9005	30912-30919	CHEBI:59132	denotes	antigen
T9006	30981-30986	NCBITaxon:10088	denotes	mouse
T9007	30987-30990	GO:0042555	denotes	Mcm
T9665	31048-31052	PR:000010246	denotes	Mcm4
T9666	31048-31056	GO:0097373	denotes	Mcm4/6/7
T9667	31110-31114	PR:000010246	denotes	Mcm4
T9668	31110-31118	GO:0097373	denotes	Mcm4/6/7
T9669	31156-31171	SO:0000984	denotes	single-stranded
T9670	31225-31240	SO:0000984	denotes	single-stranded
T9671	31255-31267	_FRAGMENT	denotes	Unwinding of
T9672	31272-31282	GO:0032508	denotes	duplex DNA
T9673	31272-31278	SO:0000985	denotes	duplex
T9674	31311-31326	SO:0000984	denotes	single-stranded
T9675	31411-31415	GO:0005657	denotes	fork
T9676	31424-31436	GO:0006272	denotes	3′-extension
T9677	31450-31454	GO:0005657	denotes	fork
T9678	31462-31466	GO:0005657	denotes	fork
T9679	31478-31490	GO:0006273	denotes	5′-extension
T9680	31543-31558	SO:0000984	denotes	single-stranded
T9681	31570-31574	PR:000010246	denotes	Mcm4
T9682	31570-31578	GO:0097373	denotes	Mcm4/6/7
T9683	31592-31601	GO:0097617	denotes	annealing
T9684	31623-31631	SO:0000988	denotes	circular
T9685	31632-31647	SO:0000984	denotes	single-stranded
T9686	31672-31677	NCBITaxon:10088	denotes	mouse
T9687	31678-31682	PR:000010246	denotes	Mcm4
T9688	31678-31686	GO:0097373	denotes	Mcm4/6/7
T9689	31697-31709	GO:0006272	denotes	3′-extension
T9690	31727-31735	NCBITaxon:2157	denotes	archaeal
T9691	31736-31739	GO:0042555	denotes	Mcm
T9692	31760-31764	PR:000010246	denotes	Mcm4
T9693	31760-31768	GO:0097373	denotes	Mcm4/6/7
T9694	31774-31781	NCBITaxon:4896	denotes	S.pombe
T9695	31786-31798	NCBITaxon:4932	denotes	S.cerevisiae
T9696	31815-31823	NCBITaxon:2157	denotes	archaeal
T9697	31824-31827	GO:0042555	denotes	Mcm
T9698	31841-31845	GO:0005657	denotes	fork
T9699	31854-31864	NCBITaxon:2759	denotes	eukaryotic
T9700	31865-31869	PR:000010246	denotes	Mcm4
T9701	31865-31873	GO:0097373	denotes	Mcm4/6/7
T9702	31908-31923	SO:0000985	denotes	double-stranded
T9703	31972-31975	GO:0042555	denotes	Mcm
T9704	32005-32011	SO:0000985	denotes	duplex
T9705	32053-32062	NCBITaxon:40674	denotes	mammalian
T9706	32063-32067	PR:000010246	denotes	Mcm4
T9707	32063-32071	GO:0097373	denotes	Mcm4/6/7
T9708	32185-32196	GO:0006260	denotes	replication
T9709	32185-32204	SO:0000296	denotes	replication origins
T9710	32230-32233	GO:0042555	denotes	Mcm
T9711	32286-32295	NCBITaxon:40674	denotes	mammalian
T9712	32296-32311	GO:0006260	denotes	DNA replication
T9713	32370-32375	NCBITaxon:9606	denotes	human
T9714	32376-32387	GO:0006260	denotes	replication
T9715	32376-32395	SO:0000296	denotes	replication origins
T9716	32408-32412	PR:000010246	denotes	Mcm4
T9717	32408-32425	GO:0097373	denotes	Mcm4/6/7 helicase
T9718	32432-32440	PR:000009861	denotes	lamin-B2
T9719	32445-32450	PR:000000084	denotes	c-myc
T9720	32451-32458	SO:0000296	denotes	origins
T9721	32493-32496	GO:0042555	denotes	Mcm
T9722	32561-32564	GO:0042555	denotes	Mcm
T9723	32572-32598	GO:0006270	denotes	DNA replication initiation
T9724	32611-32616	PR:000000084	denotes	c-myc
T9725	32710-32725	SO:0000984	denotes	single-stranded
T9726	32758-32761	GO:0042555	denotes	Mcm
T9727	33101-33105	PR:000010246	denotes	Mcm4
T9728	33101-33109	GO:0097373	denotes	Mcm4/6/7
T9729	33181-33196	SO:0000984	denotes	single-stranded
T9730	33377-33381	PR:000010246	denotes	Mcm4
T9731	33377-33385	GO:0097373	denotes	Mcm4/6/7
T9732	33443-33458	SO:0000984	denotes	single-stranded
T9733	33640-33643	GO:0042555	denotes	Mcm
T9734	33820-33830	GO:0065007	denotes	regulation
T9735	33853-33882	GO:0006270	denotes	initiation of DNA replication
T9736	33987-33998	GO:0006260	denotes	replication
T9737	33987-34005	SO:0000296	denotes	replication origin
T9738	34013-34019	SO:0001026	denotes	genome
T10619	34055-34059	PR:000010246	denotes	Mcm4
T10620	34055-34063	GO:0097373	denotes	Mcm4/6/7
T10621	34108-34112	GO:0005657	denotes	fork
T10622	34141-34156	SO:0000984	denotes	single-stranded
T10623	34192-34195	GO:0042555	denotes	Mcm
T10624	34274-34297	GO:0032508	denotes	unwinding of duplex DNA
T10625	34287-34293	SO:0000985	denotes	duplex
T10626	34341-34345	SO:0000028	denotes	pair
T10627	34353-34359	SO:0000985	denotes	duplex
T10628	34396-34399	GO:0042555	denotes	Mcm
T10629	34419-34425	SO:0000985	denotes	Duplex
T10630	34450-34455	SO:0000028	denotes	pairs
T10631	34483-34487	GO:0005657	denotes	fork
T10632	34563-34567	NCBITaxon:10633	denotes	SV40
T10633	34570-34577	CHEBI:59132	denotes	antigen
T10634	34591-34594	GO:0042555	denotes	Mcm
T10635	34645-34651	SO:0000985	denotes	duplex
T10636	34670-34678	SO:0000988	denotes	circular
T10637	34679-34694	SO:0000984	denotes	single-stranded
T10638	34709-34715	SO:0000985	denotes	duplex
T10639	34755-34759	PR:000010246	denotes	Mcm4
T10640	34755-34763	GO:0097373	denotes	Mcm4/6/7
T10641	34906-34912	SO:0000985	denotes	duplex
T10642	34923-34926	GO:0042555	denotes	Mcm
T10672	36328-36334	SO:0000985	denotes	duplex
T10673	36394-36420	SO:0000296	denotes	origins of DNA replication
T10674	36405-36420	GO:0006260	denotes	DNA replication
T10675	36447-36454	CHEBI:59132	denotes	antigen
T10676	36628-36634	GO:0032508	denotes	melted
T10677	36647-36650	GO:0042555	denotes	Mcm
T10678	36750-36768	GO:0033202	denotes	helicase complexes
T10679	36821-36828	SO:0000296	denotes	origins
T10680	36857-36878	GO:0032508	denotes	melting of duplex DNA
T10681	36868-36874	SO:0000985	denotes	duplex
T10682	36919-36924	GO:0005656	denotes	preRC
T10683	36961-36970	GO:0000785	denotes	chromatin
T10684	37061-37065	PR:000010250	denotes	Mcm8
T10685	37119-37123	GO:0005657	denotes	fork
T10686	37151-37156	PR:000005215	denotes	Cdc45
T10687	37210-37214	PR:000005218	denotes	Cdc7
T10688	37297-37301	PR:000010246	denotes	Mcm4
T10689	37297-37305	GO:0097373	denotes	Mcm4/6/7
T10690	37317-37323	SO:0000985	denotes	duplex
T10691	37379-37383	PR:000010246	denotes	Mcm4
T10692	37379-37387	GO:0097373	denotes	Mcm4/6/7
T10693	37426-37437	GO:0006260	denotes	replicative
T10694	37454-37465	GO:0006260	denotes	replication
T10695	37454-37471	GO:0005657	denotes	replication forks
T10696	37496-37500	PR:000010246	denotes	Mcm4
T10697	37496-37512	GO:0097373	denotes	Mcm4/6/7 complex
T10698	37549-37555	SO:0000985	denotes	duplex
T10699	37556-37571	GO:0006260	denotes	DNA replication
T10700	37556-37577	GO:0005657	denotes	DNA replication forks
T10701	37579-37583	PR:000010246	denotes	Mcm4
T10702	37579-37587	GO:0097373	denotes	Mcm4/6/7
T10703	37670-37673	GO:0042555	denotes	Mcm
T10704	37684-37689	PR:000005215	denotes	Cdc45
T10705	37699-37703	GO:0000811	denotes	GINS
T10706	37743-37747	PR:000010250	denotes	Mcm8
T10707	37822-37833	GO:0006260	denotes	replicating
T10708	37845-37851	SO:0001026	denotes	genome
T8758	28199-28218	SO:0000296	denotes	replication origins
T8759	28258-28269	GO:0006260	denotes	replicative
T8760	28387-28416	GO:0006270	denotes	initiation of DNA replication
T8761	28442-28445	GO:0042555	denotes	Mcm
T8762	28474-28484	NCBITaxon:2759	denotes	eukaryotic
T8763	28485-28496	GO:0006260	denotes	replicative
T8764	28594-28599	NCBITaxon:10088	denotes	mouse
T8765	28600-28604	PR:000010246	denotes	Mcm4
T8766	28600-28608	GO:0097373	denotes	Mcm4/6/7
T8767	28644-28648	GO:0005657	denotes	fork
T1405	4632-4640	CHEBI:33893	denotes	Reagents
T1406	4749-4764	SO:0000984	denotes	single-stranded
T1407	4765-4773	SO:0000988	denotes	circular
T1408	4779-4781	NCBITaxon:10665	denotes	T4
T1409	4782-4796	CHEBI:15986	denotes	polynucleotide
T1410	4782-4803	PR:000012924	denotes	polynucleotide kinase
T1411	4900-4902	GO:0042571	denotes	Ab
T1412	4904-4912	GO:0042571	denotes	antibody
T1413	4914-4921	CHEBI:2511	denotes	agarose
T1414	4956-4960	NCBITaxon:10633	denotes	SV40
T1614	5303-5313	GO:0010467	denotes	Expression
T1615	5334-5339	NCBITaxon:10088	denotes	mouse
T1616	5340-5344	PR:000010246	denotes	Mcm4
T1617	5340-5356	GO:0097373	denotes	Mcm4/6/7 complex
T1618	5373-5386	NCBITaxon:10442	denotes	baculoviruses
T1619	5387-5397	GO:0010467	denotes	expressing
T1620	5403-5407	PR:000010246	denotes	Mcm4
T1621	5408-5412	PR:000010248	denotes	Mcm6
T1622	5416-5420	PR:000010249	denotes	Mcm7
T1623	5485-5491	NCBITaxon:6960	denotes	insect
T1624	5623-5633	GO:0010467	denotes	expression
T1625	5641-5645	PR:000010246	denotes	Mcm4
T1626	5641-5649	GO:0097373	denotes	Mcm4/6/7
T1627	5709-5722	NCBITaxon:10442	denotes	baculoviruses
T1628	5723-5733	GO:0010467	denotes	expressing
T1629	5743-5747	PR:000010246	denotes	Mcm4
T1630	5748-5752	PR:000010248	denotes	Mcm6
T1631	5772-5782	GO:0010467	denotes	expressing
T1632	5787-5791	PR:000010249	denotes	Mcm7
T1633	5857-5861	PR:000010246	denotes	Mcm4
T1634	5857-5875	GO:0097373	denotes	Mcm4/6/7 complexes
T8755	28121-28132	GO:0006260	denotes	replication
T8756	28121-28138	GO:0005657	denotes	replication forks
T8757	28199-28210	GO:0006260	denotes	replication
T10643	34946-34954	SO:0000988	denotes	circular
T10644	34955-34970	SO:0000984	denotes	single-stranded
T10645	35046-35050	GO:0005657	denotes	fork
T10646	35123-35126	GO:0042555	denotes	Mcm
T10647	35210-35216	SO:0000985	denotes	duplex
T10648	35263-35266	GO:0042555	denotes	Mcm
T10649	35337-35343	SO:0000985	denotes	duplex
T10650	35358-35362	GO:0005657	denotes	fork
T10651	35390-35400	SO:0000028	denotes	base pairs
T10652	35531-35537	SO:0000985	denotes	duplex
T10653	35576-35580	PR:000010246	denotes	Mcm4
T10654	35576-35584	GO:0097373	denotes	Mcm4/6/7
T10655	35640-35645	SO:0000028	denotes	pairs
T10656	35715-35721	SO:0000985	denotes	duplex
T10657	35815-35824	SO:0000028	denotes	base pair
T10658	35854-35857	GO:0042555	denotes	Mcm
T10659	35911-35921	SO:0000028	denotes	base pairs
T10660	35959-35963	PR:000010246	denotes	Mcm4
T10661	35959-35967	GO:0097373	denotes	Mcm4/6/7
T10662	36051-36060	NCBITaxon:40674	denotes	mammalian
T10663	36061-36065	PR:000010246	denotes	Mcm4
T10664	36061-36069	GO:0097373	denotes	Mcm4/6/7
T10665	36125-36135	NCBITaxon:2759	denotes	eukaryotic
T10666	36136-36147	GO:0006260	denotes	replicative
T10667	36167-36196	GO:0006270	denotes	initiation of DNA replication
T10668	36198-36201	GO:0042555	denotes	Mcm
T10669	36265-36271	GO:0032508	denotes	melted
T10670	36272-36278	SO:0000296	denotes	origin
T10671	36315-36338	GO:0032508	denotes	unwinding of duplex DNA
R1	T58	T57	_lexicallyChainedTo	of DNA replication,initiation
R275	T566	T565	_lexicallyChainedTo	7,Mcm
R1118	T1862	T1861	_lexicallyChainedTo	extension,3′-
R2614	T3971	T3970	_lexicallyChainedTo	complexes,Formation of
R3143	T4673	T4672	_lexicallyChainedTo	DNA,unwinding of
R3462	T5140	T5139	_lexicallyChainedTo	extension,3′-
R3961	T5834	T5833	_lexicallyChainedTo	yeast,fission
R3962	T5851	T5850	_lexicallyChainedTo	6,Mcm
R3963	T5852	T5850	_lexicallyChainedTo	7,Mcm
R5995	T8984	T8983	_lexicallyChainedTo	complex,assembly of
R6453	T9672	T9671	_lexicallyChainedTo	duplex DNA,Unwinding of

2_test

Id	Subject	Object	Predicate	Lexical cue
15917436-10966472-76775476	1641-1642	10966472	denotes	1
15917436-10966472-76775477	1786-1787	10966472	denotes	1
15917436-8381400-76775478	1788-1789	8381400	denotes	2
15917436-10884341-76775479	1922-1923	10884341	denotes	3
15917436-14566326-76775479	1922-1923	14566326	denotes	3
15917436-12548282-76775479	1922-1923	12548282	denotes	3
15917436-12774115-76775480	2016-2017	12774115	denotes	6
15917436-12206765-76775481	2043-2044	12206765	denotes	7
15917436-14566326-76775482	2064-2065	14566326	denotes	4
15917436-10611290-76775483	2066-2067	10611290	denotes	8
15917436-10677495-76775484	2068-2069	10677495	denotes	9
15917436-9305914-76775485	2190-2192	9305914	denotes	10
15917436-10567526-76775485	2190-2192	10567526	denotes	10
15917436-12207017-76775485	2190-2192	12207017	denotes	10
15917436-14609960-76775485	2190-2192	14609960	denotes	10
15917436-11136247-76775485	2190-2192	11136247	denotes	10
15917436-10872463-76775486	2306-2308	10872463	denotes	15
15917436-12045100-76775487	2309-2311	12045100	denotes	16
15917436-9305914-76775488	2473-2475	9305914	denotes	10
15917436-10567526-76775488	2473-2475	10567526	denotes	10
15917436-12207017-76775488	2473-2475	12207017	denotes	10
15917436-14609960-76775488	2473-2475	14609960	denotes	10
15917436-11136247-76775488	2473-2475	11136247	denotes	10
15917436-9214646-76775489	2479-2481	9214646	denotes	17
15917436-10770926-76775489	2479-2481	10770926	denotes	17
15917436-10567526-76775490	2575-2577	10567526	denotes	11
15917436-12207017-76775490	2575-2577	12207017	denotes	11
15917436-14609960-76775490	2575-2577	14609960	denotes	11
15917436-10884341-76775491	2749-2750	10884341	denotes	3
15917436-10567526-76775492	2751-2753	10567526	denotes	11
15917436-9335335-76775493	2983-2985	9335335	denotes	19
15917436-10834843-76775494	2986-2988	10834843	denotes	20
15917436-14609960-76775495	3196-3198	14609960	denotes	13
15917436-11136247-76775496	3199-3201	11136247	denotes	14
15917436-14609960-76775497	3347-3349	14609960	denotes	13
15917436-11136247-76775498	3350-3352	11136247	denotes	14
15917436-9765408-76775499	3353-3355	9765408	denotes	21
15917436-14609960-76775500	3659-3661	14609960	denotes	13
15917436-8390661-76775501	5036-5038	8390661	denotes	22
15917436-12151393-76775502	5095-5097	12151393	denotes	23
15917436-10567526-76775503	5462-5464	10567526	denotes	11
15917436-12207017-76775504	5915-5917	12207017	denotes	12
15917436-14609960-76775505	6803-6805	14609960	denotes	13
15917436-14609960-76775506	7934-7936	14609960	denotes	13
15917436-10567526-76775507	8078-8080	10567526	denotes	11
15917436-14609960-76775508	11121-11123	14609960	denotes	13
15917436-9765408-76775509	12741-12743	9765408	denotes	21
15917436-14609960-76775510	13840-13842	14609960	denotes	13
15917436-14609960-76775511	14842-14844	14609960	denotes	13
15917436-9765408-76775512	14845-14847	9765408	denotes	21
15917436-14609960-76775513	16467-16469	14609960	denotes	13
15917436-12151393-76775514	17989-17991	12151393	denotes	23
15917436-12917421-76775515	17992-17994	12917421	denotes	25
15917436-9305914-76775516	18780-18782	9305914	denotes	10
15917436-12917421-76775517	19031-19033	12917421	denotes	25
15917436-11136247-76775518	19400-19402	11136247	denotes	14
15917436-13679365-76775519	19403-19405	13679365	denotes	26
15917436-12975364-76775520	19406-19408	12975364	denotes	27
15917436-14609960-76775521	19868-19870	14609960	denotes	13
15917436-14609960-76775522	22359-22361	14609960	denotes	13
15917436-14609960-76775523	22992-22994	14609960	denotes	13
15917436-10720330-76775524	22995-22997	10720330	denotes	28
15917436-7935372-76775525	23350-23352	7935372	denotes	29
15917436-12589000-76775526	23353-23355	12589000	denotes	30
15917436-7935372-76775527	23568-23570	7935372	denotes	29
15917436-12589000-76775528	23637-23639	12589000	denotes	30
15917436-14609960-76775529	24569-24571	14609960	denotes	13
15917436-14609960-76775530	26552-26554	14609960	denotes	13
15917436-4080553-76775531	27528-27530	4080553	denotes	31
15917436-2843291-76775532	28969-28971	2843291	denotes	32
15917436-14609960-76775533	29100-29102	14609960	denotes	13
15917436-14609960-76775534	29447-29449	14609960	denotes	13
15917436-9765408-76775535	29755-29757	9765408	denotes	21
15917436-9765408-76775536	30625-30627	9765408	denotes	21
15917436-14566326-76775537	30921-30922	14566326	denotes	4
15917436-12774115-76775538	30923-30924	12774115	denotes	6
15917436-11136247-76775539	31800-31802	11136247	denotes	14
15917436-13679365-76775540	31803-31805	13679365	denotes	26
15917436-12975364-76775541	31806-31808	12975364	denotes	27
15917436-12548282-76775542	31953-31954	12548282	denotes	5
15917436-12975364-76775543	31955-31957	12975364	denotes	27
15917436-15190069-76775544	32640-32642	15190069	denotes	33
15917436-4080553-76775545	35764-35766	4080553	denotes	31
15917436-9765408-76775546	36507-36509	9765408	denotes	21
15917436-14993926-76775547	37076-37078	14993926	denotes	34
15917436-15707891-76775547	37076-37078	15707891	denotes	34
15917436-15684404-76775547	37076-37078	15684404	denotes	34
15917436-9001208-76775548	37158-37160	9001208	denotes	37
15917436-12581157-76775549	37161-37163	12581157	denotes	38
15917436-10748114-76775550	37224-37226	10748114	denotes	39
15917436-10846177-76775551	37227-37229	10846177	denotes	40
15917436-9001208-76775552	37691-37693	9001208	denotes	37
15917436-12581157-76775553	37694-37696	12581157	denotes	38
15917436-12730134-76775554	37705-37707	12730134	denotes	41
15917436-12730133-76775555	37708-37710	12730133	denotes	42
15917436-11804324-76775556	37738-37740	11804324	denotes	43
15917436-15707891-76775557	37749-37751	15707891	denotes	35
15917436-15684404-76775558	37752-37754	15684404	denotes	36

PMC:1140370 JSONTXT

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craft-sa-dev

craft-ca-core-ex-dev

craft-ca-core-dev

2_test

PMC:1140370 JSON TXT