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PMC:1134658 / 25953-27093 JSONTXT

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pmc-enju-pas

Id Subject Object Predicate Lexical cue
T15269 0-12 JJ denotes Western-blot
T15270 13-21 NN denotes analysis
T15271 22-23 NN denotes B
T15272 24-29 NN denotes cells
T15273 30-34 IN denotes from
T15274 35-45 JJ denotes peripheral
T15275 46-51 NN denotes blood
T15276 52-54 CC denotes or
T15277 55-63 VB denotes cultured
T15278 64-74 NN denotes cell-lines
T15279 75-79 VB denotes were
T15280 80-85 VB denotes lysed
T15281 86-88 IN denotes in
T15282 89-94 NN denotes lysis
T15283 95-101 NN denotes buffer
T15284 102-103 -LRB- denotes (
T15285 103-111 NN denotes glycerol
T15286 112-114 CD denotes 10
T15287 114-115 NN denotes %
T15288 115-116 -COMMA- denotes ,
T15289 117-134 NN denotes β-mercaptoethanol
T15290 135-136 CD denotes 5
T15291 136-137 NN denotes %
T15292 137-138 -COMMA- denotes ,
T15293 139-145 CD denotes 0.0625
T15294 146-147 NN denotes M
T15295 148-156 NN denotes Tris-HCL
T15296 157-158 -LRB- denotes [
T15297 158-160 NN denotes pH
T15298 161-164 CD denotes 6.8
T15299 164-165 -RRB- denotes ]
T15300 165-166 -COMMA- denotes ,
T15301 167-173 NN denotes sodium
T15302 174-181 NN denotes dodecyl
T15303 182-190 NN denotes sulphate
T15304 191-192 -LRB- denotes [
T15305 192-195 NN denotes SDS
T15306 195-196 -RRB- denotes ]
T15307 197-200 CD denotes 2.5
T15308 200-201 NN denotes %
T15309 201-206 NN denotes w/vol
T15310 206-207 -RRB- denotes )
T15311 209-214 JJ denotes Total
T15312 215-222 NN denotes protein
T15313 223-224 -LRB- denotes (
T15314 224-230 CD denotes 30–100
T15315 231-233 NN denotes μg
T15316 233-234 -RRB- denotes )
T15317 235-239 IN denotes from
T15318 240-244 DT denotes each
T15319 245-251 NN denotes sample
T15320 252-255 VB denotes was
T15321 256-259 VB denotes run
T15322 260-262 IN denotes on
T15323 263-265 CD denotes 10
T15324 265-266 NN denotes %
T15325 267-269 CC denotes or
T15326 270-272 CD denotes 12
T15327 272-273 NN denotes %
T15328 274-292 NN denotes SDS/polyacrylamide
T15329 293-294 -LRB- denotes (
T15330 294-302 NN denotes SDS/PAGE
T15331 302-303 -RRB- denotes )
T15332 304-308 NN denotes gels
T15333 309-312 CC denotes and
T15334 313-320 VB denotes blotted
T15335 321-325 IN denotes onto
T15336 326-340 NN denotes nitrocellulose
T15337 341-348 NN denotes filters
T15338 349-350 -LRB- denotes (
T15339 350-357 NNP denotes Protran
T15340 357-358 -COLON- denotes ;
T15341 359-369 NNP denotes Schleicher
T15342 370-371 CC denotes &
T15343 371-378 NNP denotes Schuell
T15344 379-383 NNP denotes GmbH
T15345 383-384 -COMMA- denotes ,
T15346 385-391 NNP denotes Dassel
T15347 391-392 -COMMA- denotes ,
T15348 393-400 NNP denotes Germany
T15349 400-401 -RRB- denotes )
T15350 403-411 NN denotes Blocking
T15351 411-412 -COMMA- denotes ,
T15352 413-420 NN denotes washing
T15353 421-424 CC denotes and
T15354 425-435 NN denotes incubation
T15355 436-438 IN denotes of
T15356 439-442 DT denotes the
T15357 443-450 NN denotes filters
T15358 451-455 IN denotes with
T15359 456-463 JJ denotes primary
T15360 464-474 NN denotes antibodies
T15361 475-479 VB denotes were
T15362 480-484 VB denotes done
T15363 485-494 VB denotes according
T15364 495-497 TO denotes to
T15365 498-501 DT denotes the
T15366 502-514 NN denotes manufacturer
T15367 514-516 POS denotes 's
T15368 517-526 NN denotes protocols
T15369 527-529 IN denotes at
T15370 530-534 NN denotes room
T15371 535-546 NN denotes temperature
T15372 547-548 -LRB- denotes (
T15373 548-550 NN denotes RT
T15374 550-551 -RRB- denotes )
T15375 553-558 IN denotes After
T15376 559-566 VB denotes washing
T15377 567-571 IN denotes with
T15378 572-579 NN denotes TBS/0.1
T15379 579-580 NN denotes %
T15380 581-589 CD denotes Tween-20
T15381 590-591 -LRB- denotes (
T15382 591-596 NN denotes TBS-T
T15383 596-597 -RRB- denotes )
T15384 597-598 -COMMA- denotes ,
T15385 599-602 DT denotes the
T15386 603-610 NN denotes filters
T15387 611-615 VB denotes were
T15388 616-625 VB denotes incubated
T15389 626-630 IN denotes with
T15390 631-642 NN denotes horseradish
T15391 643-653 NN denotes peroxidase
T15392 654-655 -LRB- denotes (
T15393 655-658 NN denotes HRP
T15394 658-659 -RRB- denotes )
T15395 660-667 VB denotes coupled
T15396 668-670 TO denotes to
T15397 671-679 JJ denotes relevant
T15398 680-689 JJ denotes secondary
T15399 690-700 NN denotes antibodies
T15400 701-702 -LRB- denotes (
T15401 702-705 VB denotes see
T15402 706-711 IN denotes above
T15403 711-712 -RRB- denotes )
T15404 713-716 IN denotes for
T15405 717-719 CD denotes 60
T15406 720-727 NN denotes minutes
T15407 728-730 IN denotes at
T15408 731-733 NN denotes RT
T15409 735-741 NN denotes Enzyme
T15410 742-750 NN denotes activity
T15411 751-754 VB denotes was
T15412 755-765 VB denotes visualised
T15413 766-768 IN denotes by
T15414 769-772 DT denotes the
T15415 773-781 VB denotes enhanced
T15416 782-799 NN denotes chemiluminescence
T15417 800-806 NN denotes system
T15418 806-807 -COMMA- denotes ,
T15419 808-816 NN denotes ECL+PLUS
T15420 817-818 -LRB- denotes (
T15421 818-826 NNP denotes Amersham
T15422 826-827 -COMMA- denotes ,
T15423 828-843 NNP denotes Buckinghamshire
T15424 843-844 -COMMA- denotes ,
T15425 845-847 NNP denotes UK
T15426 847-848 -RRB- denotes )
T15427 850-863 JJ denotes Densitometric
T15428 864-872 NN denotes analysis
T15429 873-876 VB denotes was
T15430 877-886 VB denotes performed
T15431 887-889 IN denotes by
T15432 890-898 VB denotes scanning
T15433 899-909 NN denotes hyperfilms
T15434 910-912 IN denotes on
T15435 913-914 DT denotes a
T15436 915-923 NNP denotes Personal
T15437 924-936 NNP denotes Densitometer
T15438 937-939 NNP denotes SI
T15439 940-941 -LRB- denotes (
T15440 941-950 NNP denotes Molecular
T15441 951-959 NNP denotes Dynamics
T15442 959-960 -COMMA- denotes ,
T15443 961-970 NNP denotes Sunnyvale
T15444 970-971 -COMMA- denotes ,
T15445 972-974 NNP denotes CA
T15446 974-975 -RRB- denotes )
T15447 977-991 NN denotes Quantification
T15448 992-994 IN denotes of
T15449 995-998 NN denotes Id1
T15450 998-999 -COMMA- denotes ,
T15451 1000-1003 NN denotes Id2
T15452 1004-1007 CC denotes and
T15453 1008-1011 NN denotes Id3
T15454 1012-1019 NN denotes protein
T15455 1020-1023 VB denotes was
T15456 1024-1034 VB denotes calculated
T15457 1035-1037 IN denotes by
T15458 1038-1049 VB denotes normalizing
T15459 1050-1053 DT denotes the
T15460 1054-1062 JJ denotes specific
T15461 1063-1070 NN denotes protein
T15462 1071-1076 NN denotes bands
T15463 1077-1079 TO denotes to
T15464 1080-1087 NN denotes β-actin
T15465 1088-1093 VB denotes using
T15466 1094-1099 NN denotes Image
T15467 1100-1105 JJ denotes Quant
T15468 1106-1109 CD denotes 5.5
T15469 1110-1118 NN denotes software
T15470 1119-1120 -LRB- denotes (
T15471 1120-1129 NNP denotes Molecular
T15472 1130-1138 NNP denotes Dynamics
T15473 1138-1139 -RRB- denotes )
R11752 T15270 T15269 arg1Of analysis,Western-blot
R11753 T15272 T15271 arg1Of cells,B
R11754 T15272 T15273 arg1Of cells,from
R11755 T15272 T15279 arg1Of cells,were
R11756 T15272 T15280 arg2Of cells,lysed
R11757 T15275 T15274 arg1Of blood,peripheral
R11758 T15275 T15276 arg1Of blood,or
R11759 T15276 T15273 arg2Of or,from
R11760 T15278 T15276 arg2Of cell-lines,or
R11761 T15278 T15277 arg2Of cell-lines,cultured
R11762 T15280 T15279 arg2Of lysed,were
R11763 T15280 T15281 arg1Of lysed,in
R11764 T15283 T15281 arg2Of buffer,in
R11765 T15283 T15282 arg1Of buffer,lysis
R11766 T15283 T15284 arg1Of buffer,(
R11767 T15287 T15285 arg1Of %,glycerol
R11768 T15287 T15286 arg1Of %,10
R11769 T15287 T15288 arg1Of %,","
R11770 T15288 T15292 arg1Of ",",","
R11771 T15291 T15288 arg2Of %,","
R11772 T15291 T15289 arg1Of %,β-mercaptoethanol
R11773 T15291 T15290 arg1Of %,5
R11774 T15292 T15300 arg1Of ",",","
R11775 T15293 T15294 arg1Of 0.0625,M
R11776 T15295 T15292 arg2Of Tris-HCL,","
R11777 T15295 T15293 arg1Of Tris-HCL,0.0625
R11778 T15295 T15296 arg1Of Tris-HCL,[
R11779 T15297 T15296 arg2Of pH,[
R11780 T15297 T15298 arg1Of pH,6.8
R11781 T15299 T15296 arg3Of ],[
R11782 T15300 T15284 arg2Of ",",(
R11783 T15305 T15304 arg2Of SDS,[
R11784 T15306 T15304 arg3Of ],[
R11785 T15307 T15308 arg1Of 2.5,%
R11786 T15309 T15300 arg2Of w/vol,","
R11787 T15309 T15301 arg1Of w/vol,sodium
R11788 T15309 T15302 arg1Of w/vol,dodecyl
R11789 T15309 T15303 arg1Of w/vol,sulphate
R11790 T15309 T15304 arg1Of w/vol,[
R11791 T15309 T15307 arg1Of w/vol,2.5
R11792 T15310 T15284 arg3Of ),(
R11793 T15312 T15311 arg1Of protein,Total
R11794 T15312 T15313 arg1Of protein,(
R11795 T15312 T15317 arg1Of protein,from
R11796 T15312 T15320 arg1Of protein,was
R11797 T15312 T15321 arg2Of protein,run
R11798 T15312 T15334 arg1Of protein,blotted
R11799 T15315 T15313 arg2Of μg,(
R11800 T15315 T15314 arg1Of μg,30–100
R11801 T15316 T15313 arg3Of ),(
R11802 T15319 T15317 arg2Of sample,from
R11803 T15319 T15318 arg1Of sample,each
R11804 T15321 T15320 arg2Of run,was
R11805 T15321 T15322 arg1Of run,on
R11806 T15321 T15333 arg1Of run,and
R11807 T15324 T15323 arg1Of %,10
R11808 T15324 T15325 arg1Of %,or
R11809 T15325 T15327 arg1Of or,%
R11810 T15326 T15325 arg2Of 12,or
R11811 T15330 T15329 arg2Of SDS/PAGE,(
R11812 T15331 T15329 arg3Of ),(
R11813 T15332 T15322 arg2Of gels,on
R11814 T15332 T15324 arg1Of gels,%
R11815 T15332 T15326 arg1Of gels,12
R11816 T15332 T15328 arg1Of gels,SDS/polyacrylamide
R11817 T15332 T15329 arg1Of gels,(
R11818 T15333 T15338 arg1Of and,(
R11819 T15334 T15333 arg2Of blotted,and
R11820 T15334 T15335 arg1Of blotted,onto
R11821 T15337 T15335 arg2Of filters,onto
R11822 T15337 T15336 arg1Of filters,nitrocellulose
R11823 T15339 T15340 arg1Of Protran,;
R11824 T15340 T15338 arg2Of ;,(
R11825 T15344 T15340 arg2Of GmbH,;
R11826 T15344 T15341 arg1Of GmbH,Schleicher
R11827 T15344 T15342 arg1Of GmbH,&
R11828 T15344 T15343 arg1Of GmbH,Schuell
R11829 T15344 T15345 arg1Of GmbH,","
R11830 T15346 T15345 arg2Of Dassel,","
R11831 T15346 T15347 arg1Of Dassel,","
R11832 T15348 T15347 arg2Of Germany,","
R11833 T15349 T15338 arg3Of ),(
R11834 T15350 T15351 arg1Of Blocking,","
R11835 T15351 T15353 arg1Of ",",and
R11836 T15352 T15351 arg2Of washing,","
R11837 T15353 T15355 arg1Of and,of
R11838 T15353 T15361 arg1Of and,were
R11839 T15353 T15362 arg2Of and,done
R11840 T15354 T15353 arg2Of incubation,and
R11841 T15357 T15355 arg2Of filters,of
R11842 T15357 T15356 arg1Of filters,the
R11843 T15357 T15358 arg1Of filters,with
R11844 T15360 T15358 arg2Of antibodies,with
R11845 T15360 T15359 arg1Of antibodies,primary
R11846 T15362 T15361 arg2Of done,were
R11847 T15362 T15363 arg1Of done,according
R11848 T15362 T15369 arg1Of done,at
R11849 T15364 T15363 arg2Of to,according
R11850 T15366 T15365 arg1Of manufacturer,the
R11851 T15366 T15367 arg2Of manufacturer,'s
R11852 T15368 T15364 arg2Of protocols,to
R11853 T15368 T15367 arg1Of protocols,'s
R11854 T15371 T15369 arg2Of temperature,at
R11855 T15371 T15370 arg1Of temperature,room
R11856 T15371 T15372 arg1Of temperature,(
R11857 T15373 T15372 arg2Of RT,(
R11858 T15374 T15372 arg3Of ),(
R11859 T15376 T15375 arg2Of washing,After
R11860 T15376 T15377 arg1Of washing,with
R11861 T15379 T15377 arg2Of %,with
R11862 T15379 T15378 arg1Of %,TBS/0.1
R11863 T15379 T15380 arg1Of %,Tween-20
R11864 T15379 T15381 arg1Of %,(
R11865 T15382 T15381 arg2Of TBS-T,(
R11866 T15383 T15381 arg3Of ),(
R11867 T15386 T15385 arg1Of filters,the
R11868 T15386 T15387 arg1Of filters,were
R11869 T15386 T15388 arg2Of filters,incubated
R11870 T15388 T15376 arg2Of incubated,washing
R11871 T15388 T15384 arg1Of incubated,","
R11872 T15388 T15387 arg2Of incubated,were
R11873 T15388 T15389 arg1Of incubated,with
R11874 T15391 T15389 arg2Of peroxidase,with
R11875 T15391 T15390 arg1Of peroxidase,horseradish
R11876 T15391 T15392 arg1Of peroxidase,(
R11877 T15391 T15395 arg2Of peroxidase,coupled
R11878 T15393 T15392 arg2Of HRP,(
R11879 T15394 T15392 arg3Of ),(
R11880 T15395 T15396 arg1Of coupled,to
R11881 T15399 T15396 arg2Of antibodies,to
R11882 T15399 T15397 arg1Of antibodies,relevant
R11883 T15399 T15398 arg1Of antibodies,secondary
R11884 T15401 T15375 arg1Of see,After
R11885 T15401 T15400 arg1Of see,(
R11886 T15401 T15402 arg1Of see,above
R11887 T15401 T15403 arg1Of see,)
R11888 T15401 T15404 arg1Of see,for
R11889 T15406 T15404 arg2Of minutes,for
R11890 T15406 T15405 arg1Of minutes,60
R11891 T15406 T15407 arg1Of minutes,at
R11892 T15408 T15401 arg2Of RT,see
R11893 T15410 T15409 arg1Of activity,Enzyme
R11894 T15410 T15411 arg1Of activity,was
R11895 T15410 T15412 arg2Of activity,visualised
R11896 T15412 T15411 arg2Of visualised,was
R11897 T15412 T15420 arg1Of visualised,(
R11898 T15417 T15412 arg1Of system,visualised
R11899 T15417 T15413 arg2Of system,by
R11900 T15417 T15414 arg1Of system,the
R11901 T15417 T15415 arg2Of system,enhanced
R11902 T15417 T15416 arg1Of system,chemiluminescence
R11903 T15417 T15418 arg1Of system,","
R11904 T15419 T15418 arg2Of ECL+PLUS,","
R11905 T15421 T15422 arg1Of Amersham,","
R11906 T15422 T15420 arg2Of ",",(
R11907 T15422 T15424 arg1Of ",",","
R11908 T15423 T15422 arg2Of Buckinghamshire,","
R11909 T15425 T15424 arg2Of UK,","
R11910 T15426 T15420 arg3Of ),(
R11911 T15428 T15427 arg1Of analysis,Densitometric
R11912 T15428 T15429 arg1Of analysis,was
R11913 T15428 T15430 arg2Of analysis,performed
R11914 T15430 T15429 arg2Of performed,was
R11915 T15433 T15430 arg1Of hyperfilms,performed
R11916 T15433 T15431 arg2Of hyperfilms,by
R11917 T15433 T15432 arg1Of hyperfilms,scanning
R11918 T15433 T15434 arg1Of hyperfilms,on
R11919 T15438 T15434 arg2Of SI,on
R11920 T15438 T15435 arg1Of SI,a
R11921 T15438 T15436 arg1Of SI,Personal
R11922 T15438 T15437 arg1Of SI,Densitometer
R11923 T15438 T15439 arg1Of SI,(
R11924 T15441 T15439 arg2Of Dynamics,(
R11925 T15441 T15440 arg1Of Dynamics,Molecular
R11926 T15441 T15442 arg1Of Dynamics,","
R11927 T15443 T15442 arg2Of Sunnyvale,","
R11928 T15443 T15444 arg1Of Sunnyvale,","
R11929 T15445 T15444 arg2Of CA,","
R11930 T15446 T15439 arg3Of ),(
R11931 T15447 T15448 arg1Of Quantification,of
R11932 T15447 T15455 arg1Of Quantification,was
R11933 T15447 T15456 arg2Of Quantification,calculated
R11934 T15449 T15450 arg1Of Id1,","
R11935 T15450 T15452 arg1Of ",",and
R11936 T15451 T15450 arg2Of Id2,","
R11937 T15453 T15452 arg2Of Id3,and
R11938 T15454 T15448 arg2Of protein,of
R11939 T15454 T15449 arg1Of protein,Id1
R11940 T15454 T15451 arg1Of protein,Id2
R11941 T15454 T15453 arg1Of protein,Id3
R11942 T15456 T15455 arg2Of calculated,was
R11943 T15456 T15457 arg1Of calculated,by
R11944 T15458 T15457 arg2Of normalizing,by
R11945 T15462 T15458 arg2Of bands,normalizing
R11946 T15462 T15459 arg1Of bands,the
R11947 T15462 T15460 arg1Of bands,specific
R11948 T15462 T15461 arg1Of bands,protein
R11949 T15462 T15463 arg1Of bands,to
R11950 T15462 T15465 arg1Of bands,using
R11951 T15464 T15463 arg2Of β-actin,to
R11952 T15469 T15465 arg2Of software,using
R11953 T15469 T15466 arg1Of software,Image
R11954 T15469 T15467 arg1Of software,Quant
R11955 T15469 T15468 arg1Of software,5.5
R11956 T15469 T15470 arg1Of software,(
R11957 T15472 T15470 arg2Of Dynamics,(
R11958 T15472 T15471 arg1Of Dynamics,Molecular
R11959 T15473 T15470 arg3Of ),(

UBERON-AE

Id Subject Object Predicate Lexical cue
T15250 46-51 http://purl.obolibrary.org/obo/UBERON_0000178 denotes blood

GO-BP

Id Subject Object Predicate Lexical cue
T15263 89-94 http://purl.obolibrary.org/obo/GO_0019835 denotes lysis
T15264 182-190 http://purl.obolibrary.org/obo/GO_0051923 denotes sulphate
T15265 548-550 http://purl.obolibrary.org/obo/GO_0003964 denotes RT
T15266 731-733 http://purl.obolibrary.org/obo/GO_0003964 denotes RT
T15267 735-750 http://purl.obolibrary.org/obo/GO_0003824 denotes Enzyme activity
T15268 972-974 http://purl.obolibrary.org/obo/GO_0033968 denotes CA

GO-MF

Id Subject Object Predicate Lexical cue
T15487 464-474 http://purl.obolibrary.org/obo/GO_0003823 denotes antibodies
T15488 690-700 http://purl.obolibrary.org/obo/GO_0003823 denotes antibodies
T15489 548-550 http://purl.obolibrary.org/obo/GO_0003964 denotes RT
T15490 731-733 http://purl.obolibrary.org/obo/GO_0003964 denotes RT
T15491 735-750 http://purl.obolibrary.org/obo/GO_0003824 denotes Enzyme activity
T15492 972-974 http://purl.obolibrary.org/obo/GO_0033968 denotes CA

GO-CC

Id Subject Object Predicate Lexical cue
T15493 24-29 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T15494 64-68 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T15495 464-474 http://purl.obolibrary.org/obo/GO_0019815 denotes antibodies
T15496 690-700 http://purl.obolibrary.org/obo/GO_0019815 denotes antibodies
T15497 464-474 http://purl.obolibrary.org/obo/GO_0042571 denotes antibodies
T15498 690-700 http://purl.obolibrary.org/obo/GO_0042571 denotes antibodies

sentences

Id Subject Object Predicate Lexical cue
T15255 0-21 Sentence denotes Western-blot analysis
T15256 22-208 Sentence denotes B cells from peripheral blood or cultured cell-lines were lysed in lysis buffer (glycerol 10%, β-mercaptoethanol 5%, 0.0625 M Tris-HCL [pH 6.8], sodium dodecyl sulphate [SDS] 2.5%w/vol).
T15257 209-402 Sentence denotes Total protein (30–100 μg) from each sample was run on 10% or 12% SDS/polyacrylamide (SDS/PAGE) gels and blotted onto nitrocellulose filters (Protran; Schleicher &Schuell GmbH, Dassel, Germany).
T15258 403-552 Sentence denotes Blocking, washing and incubation of the filters with primary antibodies were done according to the manufacturer's protocols at room temperature (RT).
T15259 553-734 Sentence denotes After washing with TBS/0.1% Tween-20 (TBS-T), the filters were incubated with horseradish peroxidase (HRP) coupled to relevant secondary antibodies (see above) for 60 minutes at RT.
T15260 735-849 Sentence denotes Enzyme activity was visualised by the enhanced chemiluminescence system, ECL+PLUS (Amersham, Buckinghamshire, UK).
T15261 850-976 Sentence denotes Densitometric analysis was performed by scanning hyperfilms on a Personal Densitometer SI (Molecular Dynamics, Sunnyvale, CA).
T15262 977-1140 Sentence denotes Quantification of Id1, Id2 and Id3 protein was calculated by normalizing the specific protein bands to β-actin using Image Quant 5.5 software (Molecular Dynamics).
T194 0-21 Sentence denotes Western-blot analysis
T195 22-208 Sentence denotes B cells from peripheral blood or cultured cell-lines were lysed in lysis buffer (glycerol 10%, β-mercaptoethanol 5%, 0.0625 M Tris-HCL [pH 6.8], sodium dodecyl sulphate [SDS] 2.5%w/vol).
T196 209-402 Sentence denotes Total protein (30–100 μg) from each sample was run on 10% or 12% SDS/polyacrylamide (SDS/PAGE) gels and blotted onto nitrocellulose filters (Protran; Schleicher &Schuell GmbH, Dassel, Germany).
T197 403-552 Sentence denotes Blocking, washing and incubation of the filters with primary antibodies were done according to the manufacturer's protocols at room temperature (RT).
T198 553-734 Sentence denotes After washing with TBS/0.1% Tween-20 (TBS-T), the filters were incubated with horseradish peroxidase (HRP) coupled to relevant secondary antibodies (see above) for 60 minutes at RT.
T199 735-849 Sentence denotes Enzyme activity was visualised by the enhanced chemiluminescence system, ECL+PLUS (Amersham, Buckinghamshire, UK).
T200 850-976 Sentence denotes Densitometric analysis was performed by scanning hyperfilms on a Personal Densitometer SI (Molecular Dynamics, Sunnyvale, CA).
T201 977-1140 Sentence denotes Quantification of Id1, Id2 and Id3 protein was calculated by normalizing the specific protein bands to β-actin using Image Quant 5.5 software (Molecular Dynamics).

events-check-again

Id Subject Object Predicate Lexical cue
T15514 995-998 Protein denotes Id1
T15515 1000-1003 Protein denotes Id2
T15516 1008-1011 Protein denotes Id3
T15517 1080-1087 Protein denotes β-actin

bionlp-st-ge-2016-reference-tees

Id Subject Object Predicate Lexical cue
T15499 631-653 Protein denotes horseradish peroxidase
T15500 655-658 Protein denotes HRP
T15501 731-733 Protein denotes RT
T15502 995-998 Protein denotes Id1
T15503 1000-1003 Protein denotes Id2
T15504 1008-1019 Protein denotes Id3 protein
T15505 1080-1087 Protein denotes β-actin

bionlp-st-ge-2016-reference

Id Subject Object Predicate Lexical cue
T15251 995-998 Protein denotes Id1
T15252 1000-1003 Protein denotes Id2
T15253 1008-1011 Protein denotes Id3
T15254 1080-1087 Protein denotes β-actin

bionlp-st-ge-2016-uniprot

Id Subject Object Predicate Lexical cue
T15474 158-160 P0A7Z4 denotes pH
T15475 995-998 P41134 denotes Id1
T15476 1000-1003 Q02363 denotes Id2
T15477 1008-1011 Q02535 denotes Id3
T15478 1080-1087 P60709 denotes β-actin

test2

Id Subject Object Predicate Lexical cue
T15245 977-991 Negative_regulation denotes Quantification
T15246 995-998 Protein denotes Id1
T15247 1000-1003 Protein denotes Id2
T15248 1008-1011 Protein denotes Id3
T15249 1080-1087 Protein denotes β-actin
R11749 T15246 T15245 themeOf Id1,Quantification
R11750 T15247 T15245 themeOf Id2,Quantification
R11751 T15248 T15245 themeOf Id3,Quantification

tees-test

Id Subject Object Predicate Lexical cue
T2978 55-68 CL:0000010 denotes cultured cell
T2979 89-94 GO:0019835 denotes lysis
T2980 95-101 CHEBI:35225 denotes buffer
T2981 103-111 D005990 denotes glycerol
T2982 103-111 CHEBI:17754 denotes glycerol
T2983 103-111 D005990 denotes glycerol
T2984 117-134 CHEBI:41218 denotes β-mercaptoethanol
T2985 148-152 CHEBI:9754 denotes Tris
T2986 148-152 PR:Q9NQV6 denotes Tris
T2987 148-152 PR:000013180 denotes Tris
T2988 148-152 PR:Q3UTQ7 denotes Tris
T2989 153-156 CHEBI:17883 denotes HCL
T2990 158-162 CVCL_UA31 denotes pH 6
T2991 167-190 CHEBI:8984 denotes sodium dodecyl sulphate
T2992 192-195 CHEBI:8984 denotes SDS
T2993 192-195 PR:000014570 denotes SDS
T2994 192-195 B5HDJ6 denotes SDS
T2995 192-195 PR:000014571 denotes SDS
T2996 192-195 PR:P09367 denotes SDS
T2997 192-195 PR:P20132 denotes SDS
T2998 192-195 PR:Q8R238 denotes SDS
T2999 192-195 PR:Q8VBT2 denotes SDS
T3000 192-195 PR:Q86B06 denotes SDS
T3001 192-195 PR:Q1PFW3 denotes SDS
T3002 192-195 D000081003 denotes SDS
T3003 215-222 GO:0003675 denotes protein
T3004 215-222 CHEBI:36080 denotes protein
T3005 215-222 CHEBI:11122 denotes protein
T3006 215-222 PR:000000001 denotes protein
T3007 215-222 SO:0000104 denotes protein
T3008 274-277 CHEBI:8984 denotes SDS
T3009 274-277 PR:000014570 denotes SDS
T3010 274-277 B5HDJ6 denotes SDS
T3011 274-277 PR:000014571 denotes SDS
T3012 274-277 PR:P09367 denotes SDS
T3013 274-277 PR:P20132 denotes SDS
T3014 274-277 PR:Q8R238 denotes SDS
T3015 274-277 PR:Q8VBT2 denotes SDS
T3016 274-277 PR:Q86B06 denotes SDS
T3017 274-277 PR:Q1PFW3 denotes SDS
T3018 274-277 D000081003 denotes SDS
T3019 278-292 CHEBI:51135 denotes polyacrylamide
T3020 278-292 CHEBI:53656 denotes polyacrylamide
T3021 278-292 C016679 denotes polyacrylamide
T3022 278-292 CHEBI:60766 denotes polyacrylamide
T3023 294-297 CHEBI:8984 denotes SDS
T3024 294-297 PR:000014570 denotes SDS
T3025 294-297 B5HDJ6 denotes SDS
T3026 294-297 PR:000014571 denotes SDS
T3027 294-297 PR:P09367 denotes SDS
T3028 294-297 PR:P20132 denotes SDS
T3029 294-297 PR:Q8R238 denotes SDS
T3030 294-297 PR:Q8VBT2 denotes SDS
T3031 294-297 PR:Q86B06 denotes SDS
T3032 294-297 PR:Q1PFW3 denotes SDS
T3033 294-297 D000081003 denotes SDS
T3034 304-308 D005782 denotes gels
T3035 304-308 D005782 denotes gels
T3036 326-340 CHEBI:53325 denotes nitrocellulose
T3037 326-340 D003101 denotes nitrocellulose
T3038 326-340 1425879 denotes nitrocellulose
T3039 464-474 D000906 denotes antibodies
T3040 572-575 CHEBI:127105 denotes TBS
T3041 581-589 CHEBI:9784 denotes Tween-20
T3042 581-589 D011136 denotes Tween-20
T3043 581-589 D011136 denotes Tween-20
T3044 581-589 CHEBI:53424 denotes Tween-20
T3045 591-594 CHEBI:127105 denotes TBS
T3046 631-642 3704 denotes horseradish
T3047 631-642 D031215 denotes horseradish
T3048 631-642 1011043 denotes horseradish
T3049 631-653 D006735 denotes horseradish peroxidase
T3050 631-658 GO:0004601 denotes horseradish peroxidase (HRP
T3051 643-653 1600930 denotes peroxidase
T3052 643-653 P15984 denotes peroxidase
T3053 643-653 Q9SCX8 denotes peroxidase
T3054 643-653 P85957 denotes peroxidase
T3055 643-653 Q01603 denotes peroxidase
T3056 643-653 Q55080 denotes peroxidase
T3057 643-653 P85317 denotes peroxidase
T3058 643-653 Q05855 denotes peroxidase
T3059 643-653 A8NK72 denotes peroxidase
T3060 643-653 P84714 denotes peroxidase
T3061 643-653 P28313 denotes peroxidase
T3062 643-653 P28314 denotes peroxidase
T3063 643-653 P16147 denotes peroxidase
T3064 643-653 B3EWG7 denotes peroxidase
T3065 655-658 PR:Q0V967 denotes HRP
T3066 655-658 Q08168 denotes HRP
T3067 690-700 D000906 denotes antibodies
T3068 735-750 GO:0003824 denotes Enzyme activity
T3069 808-811 P55797 denotes ECL
T3070 808-811 PR:P55797 denotes ECL
T3071 808-811 P83410 denotes ECL
T3072 808-811 CL:0000504 denotes ECL
T3073 995-998 PR:P41134 denotes Id1
T3074 995-998 PR:P20067 denotes Id1
T3075 995-998 PR:P41135 denotes Id1
T3076 995-998 PR:000000039 denotes Id1
T3077 1000-1003 PR:000000040 denotes Id2
T3078 1000-1003 PR:P41137 denotes Id2
T3079 1000-1003 PR:Q02363 denotes Id2
T3080 1000-1003 PR:P41136 denotes Id2
T3081 1000-1003 C039556 denotes Id2
T3082 1000-1003 C039556 denotes Id2
T3083 1008-1011 PR:P41133 denotes Id3
T3084 1008-1011 PR:P41138 denotes Id3
T3085 1008-1011 PR:Q02535 denotes Id3
T3086 1008-1011 PR:000000041 denotes Id3
T3087 1012-1019 PR:000000001 denotes protein
T3088 1012-1019 CHEBI:36080 denotes protein
T3089 1012-1019 CHEBI:11122 denotes protein
T3090 1012-1019 GO:0003675 denotes protein
T3091 1012-1019 SO:0000104 denotes protein
T3092 1063-1070 PR:000000001 denotes protein
T3093 1063-1070 CHEBI:36080 denotes protein
T3094 1063-1070 CHEBI:11122 denotes protein
T3095 1063-1070 GO:0003675 denotes protein
T3096 1063-1070 SO:0000104 denotes protein
T3097 1080-1087 O42161 denotes β-actin
T3098 1080-1087 Q5R1X3 denotes β-actin
T3099 1080-1087 Q9Y702 denotes β-actin
T3100 1080-1087 P60706 denotes β-actin
T3101 1080-1087 P29751 denotes β-actin
T3102 1080-1087 P68143 denotes β-actin
T3103 1080-1087 P83750 denotes β-actin
T3104 1080-1087 P60710 denotes β-actin
T3105 1080-1087 Q71FK5 denotes β-actin
T3106 1080-1087 P60711 denotes β-actin
T3107 1080-1087 Q76N69 denotes β-actin
T3108 1080-1087 P79818 denotes β-actin
T3109 1080-1087 P60713 denotes β-actin
T3110 1080-1087 PR:000003676 denotes β-actin
T3111 1080-1087 Q0PGG4 denotes β-actin
T3112 1080-1087 Q6QAQ1 denotes β-actin
T3113 1080-1087 P84336 denotes β-actin
T3114 1080-1087 O93400 denotes β-actin
T3115 1080-1087 Q711N9 denotes β-actin
T3116 1080-1087 O18840 denotes β-actin
T3117 1080-1087 P48975 denotes β-actin
T3118 1080-1087 Q4L0Y2 denotes β-actin
T3119 1080-1087 Q5R6G0 denotes β-actin
T3120 1080-1087 P83751 denotes β-actin
T3121 1080-1087 P60707 denotes β-actin
T3122 1080-1087 P60709 denotes β-actin
T3123 1080-1087 P60712 denotes β-actin
T3124 1080-1087 Q91ZK5 denotes β-actin
T3125 1080-1087 P15475 denotes β-actin
T3126 1080-1087 Q6NVA9 denotes β-actin
T3127 1080-1087 P84856 denotes β-actin
T3128 1080-1087 Q4R561 denotes β-actin
T3129 1080-1087 P60708 denotes β-actin
T3130 1080-1087 D000199 denotes β-actin