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PMC:1134658 / 22781-29525 JSONTXT

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pmc-enju-pas

Id Subject Object Predicate Lexical cue
T13708 9-13 NN denotes Cell
T13709 14-21 NN denotes culture
T13710 22-24 IN denotes If
T13711 25-28 RB denotes not
T13712 29-38 VB denotes specified
T13713 38-39 -COMMA- denotes ,
T13714 40-43 DT denotes all
T13715 44-49 NN denotes cells
T13716 50-54 VB denotes were
T13717 55-63 VB denotes cultured
T13718 64-66 IN denotes in
T13719 67-73 NN denotes X-VIVO
T13720 74-77 CD denotes 15™
T13721 78-79 -LRB- denotes (
T13722 79-91 NNP denotes BioWhittaker
T13723 91-92 -COMMA- denotes ,
T13724 93-101 NNP denotes Verviers
T13725 101-102 -COMMA- denotes ,
T13726 103-110 NNP denotes Belgium
T13727 110-111 -RRB- denotes )
T13728 112-122 JJ denotes serum-free
T13729 123-129 NN denotes medium
T13730 130-132 IN denotes at
T13731 133-137 NN denotes 37°C
T13732 138-141 CC denotes and
T13733 142-143 CD denotes 5
T13734 143-144 NN denotes %
T13735 145-148 NN denotes CO2
T13736 149-151 IN denotes in
T13737 152-155 NN denotes air
T13738 157-167 JJ denotes Peripheral
T13739 168-173 NN denotes blood
T13740 174-177 VB denotes was
T13741 178-186 VB denotes provided
T13742 187-189 IN denotes by
T13743 190-193 DT denotes the
T13744 194-199 NNP denotes Blood
T13745 200-204 NNP denotes Bank
T13746 205-207 IN denotes at
T13747 208-216 NNP denotes Buskerud
T13748 217-225 NNP denotes Regional
T13749 226-234 NNP denotes Hospital
T13750 235-239 IN denotes with
T13751 240-246 JJ denotes formal
T13752 247-256 NN denotes agreement
T13753 257-259 IN denotes by
T13754 260-263 DT denotes the
T13755 264-272 NN denotes patients
T13756 272-273 -COMMA- denotes ,
T13757 274-277 CC denotes and
T13758 278-286 NN denotes approval
T13759 287-289 IN denotes by
T13760 290-293 DT denotes the
T13761 294-302 JJ denotes regional
T13762 303-309 NN denotes ethics
T13763 310-319 NN denotes committee
T13764 321-327 RB denotes Highly
T13765 328-336 VB denotes purified
T13766 337-344 VB denotes resting
T13767 345-350 JJ denotes human
T13768 351-364 NN denotes B-lymphocytes
T13769 365-366 -LRB- denotes (
T13770 366-371 JJ denotes CD19+
T13771 372-377 NN denotes cells
T13772 377-378 -RRB- denotes )
T13773 379-383 VB denotes were
T13774 384-392 VB denotes isolated
T13775 393-397 IN denotes from
T13776 398-401 DT denotes the
T13777 402-412 JJ denotes peripheral
T13778 413-418 NN denotes blood
T13779 419-421 IN denotes by
T13780 422-431 VB denotes rosetting
T13781 432-436 IN denotes with
T13782 437-451 JJ denotes immunomagnetic
T13783 452-457 NN denotes beads
T13784 458-459 -LRB- denotes (
T13785 459-468 NN denotes Dynabeads
T13786 469-473 NN denotes M450
T13787 473-474 -COLON- denotes ;
T13788 475-480 NNP denotes Dynal
T13789 480-481 -COMMA- denotes ,
T13790 482-486 NNP denotes Oslo
T13791 486-487 -COMMA- denotes ,
T13792 488-494 NNP denotes Norway
T13793 494-495 -RRB- denotes )
T13794 496-498 IN denotes as
T13795 499-508 VB denotes described
T13796 509-510 -LRB- denotes [
T13797 510-512 CD denotes 55
T13798 512-513 -RRB- denotes ]
T13799 515-519 DT denotes This
T13800 520-529 NN denotes procedure
T13801 530-536 VB denotes yields
T13802 537-541 JJ denotes less
T13803 542-546 IN denotes than
T13804 547-550 CD denotes 0.5
T13805 550-551 NN denotes %
T13806 552-553 NN denotes T
T13807 554-559 NN denotes cells
T13808 559-560 -COMMA- denotes ,
T13809 561-564 CD denotes 0.1
T13810 564-565 NN denotes %
T13811 566-568 NN denotes NK
T13812 569-574 NN denotes cells
T13813 574-575 -COMMA- denotes ,
T13814 576-579 CC denotes and
T13815 580-583 CD denotes 0.5
T13816 583-584 NN denotes %
T13817 585-594 NN denotes monocytes
T13818 595-597 IN denotes as
T13819 598-604 VB denotes judged
T13820 605-607 IN denotes by
T13821 608-616 JJ denotes indirect
T13822 617-635 NN denotes immunofluorescence
T13823 636-644 NN denotes staining
T13824 646-649 DT denotes The
T13825 650-659 VB denotes following
T13826 660-664 NN denotes cell
T13827 665-670 NN denotes lines
T13828 671-675 IN denotes from
T13829 676-681 JJ denotes human
T13830 682-690 JJ denotes lymphoid
T13831 691-703 NN denotes malignancies
T13832 704-708 VB denotes were
T13833 709-719 VB denotes maintained
T13834 720-722 IN denotes in
T13835 723-727 NN denotes RPMI
T13836 728-732 CD denotes 1640
T13837 733-734 -LRB- denotes (
T13838 734-737 NN denotes PAA
T13839 738-750 NNP denotes Laboratories
T13840 751-755 NNP denotes GmbH
T13841 755-756 -COMMA- denotes ,
T13842 757-765 NNP denotes Pasching
T13843 765-766 -COMMA- denotes ,
T13844 767-774 NNP denotes Austria
T13845 774-775 -RRB- denotes )
T13846 776-788 VB denotes supplemented
T13847 789-793 IN denotes with
T13848 794-796 CD denotes 10
T13849 796-797 NN denotes %
T13850 798-804 JJ denotes foetal
T13851 805-811 JJ denotes bovine
T13852 812-817 NN denotes serum
T13853 818-819 -LRB- denotes (
T13854 819-822 NN denotes FCS
T13855 822-823 -RRB- denotes )
T13856 823-824 -COMMA- denotes ,
T13857 825-828 CD denotes 100
T13858 829-837 NN denotes units/ml
T13859 838-848 NN denotes penicillin
T13860 849-850 NN denotes G
T13861 850-851 -COMMA- denotes ,
T13862 852-855 CC denotes and
T13863 856-859 CD denotes 100
T13864 860-868 NN denotes units/ml
T13865 869-871 IN denotes of
T13866 872-884 NN denotes streptomycin
T13867 885-893 NN denotes sulphate
T13868 893-894 -COMMA- denotes ,
T13869 895-898 CC denotes but
T13870 899-912 JJ denotes serum-starved
T13871 913-916 IN denotes for
T13872 917-919 IN denotes at
T13873 920-925 JJ denotes least
T13874 926-930 CD denotes four
T13875 931-936 NN denotes hours
T13876 937-940 CC denotes and
T13877 941-949 VB denotes cultured
T13878 950-952 IN denotes in
T13879 953-959 NN denotes X-VIVO
T13880 960-963 CD denotes 15™
T13881 964-968 WRB denotes when
T13882 969-977 VB denotes included
T13883 978-980 IN denotes in
T13884 981-992 NN denotes experiments
T13885 992-993 -COLON- denotes :
T13886 994-1006 JJ denotes EBV-negative
T13887 1007-1009 NN denotes BL
T13888 1010-1014 NN denotes cell
T13889 1015-1020 NN denotes lines
T13890 1021-1026 NN denotes Ramos
T13891 1027-1028 -LRB- denotes (
T13892 1028-1033 NN denotes ECACC
T13893 1034-1042 CD denotes 85030802
T13894 1042-1043 -RRB- denotes )
T13895 1043-1044 -COMMA- denotes ,
T13896 1045-1049 NN denotes HL60
T13897 1050-1051 -LRB- denotes (
T13898 1051-1059 NN denotes JCRB0085
T13899 1059-1060 -RRB- denotes )
T14428 1606-1616 NN denotes Antibodies
T14429 1617-1621 VB denotes used
T14430 1622-1625 IN denotes for
T14431 1626-1630 NN denotes flow
T14432 1631-1641 JJ denotes cytometric
T14433 1642-1650 NN denotes analysis
T14434 1651-1654 CC denotes and
T14435 1655-1665 NN denotes immunoblot
T14436 1666-1674 NN denotes analysis
T14437 1675-1685 NN denotes Antibodies
T14438 1686-1693 IN denotes against
T14439 1694-1697 DT denotes the
T14440 1698-1703 JJ denotes human
T14441 1704-1717 NN denotes BMP-receptors
T14442 1718-1725 NN denotes Act-RIA
T14443 1725-1726 -COMMA- denotes ,
T14444 1727-1734 NN denotes BMP-RIB
T14445 1734-1735 -COMMA- denotes ,
T14446 1736-1743 NN denotes BMPR-II
T14447 1743-1744 -COMMA- denotes ,
T14448 1745-1753 NN denotes Act-RIIA
T14449 1754-1757 CC denotes and
T14450 1758-1766 NN denotes Act-RIIb
T14451 1767-1771 VB denotes were
T14452 1772-1781 VB denotes purchased
T14453 1782-1786 IN denotes from
T14454 1787-1788 NNP denotes R
T14455 1789-1790 CC denotes &
T14456 1791-1792 NNP denotes D
T14457 1793-1800 NNP denotes Systems
T14458 1801-1802 -LRB- denotes (
T14459 1802-1810 NNP denotes Abingdon
T14460 1810-1811 -COMMA- denotes ,
T14461 1812-1814 NNP denotes UK
T14462 1814-1815 -RRB- denotes )
T14463 1817-1826 NN denotes Detection
T14464 1827-1829 IN denotes of
T14465 1830-1833 DT denotes the
T14466 1834-1847 NN denotes BMP-6-protein
T14467 1848-1851 VB denotes has
T14468 1852-1856 VB denotes been
T14469 1857-1862 VB denotes tried
T14470 1863-1867 IN denotes with
T14471 1868-1871 DT denotes the
T14472 1872-1881 VB denotes following
T14473 1882-1892 NN denotes antibodies
T14474 1892-1893 -COLON- denotes :
T14475 1894-1898 NN denotes goat
T14476 1899-1909 JJ denotes polyclonal
T14477 1910-1920 NN denotes anti-BMP-6
T14478 1921-1922 -LRB- denotes (
T14479 1922-1927 NNP denotes Santa
T14480 1928-1932 NNP denotes Cruz
T14481 1932-1933 -COMMA- denotes ,
T14482 1934-1937 NNP denotes San
T14483 1938-1943 NNP denotes Diego
T14484 1943-1944 -COMMA- denotes ,
T14485 1945-1947 NNP denotes CA
T14486 1947-1948 -COMMA- denotes ,
T14487 1949-1952 NNP denotes USA
T14488 1952-1953 -RRB- denotes )
T14489 1953-1954 -COMMA- denotes ,
T14490 1955-1965 JJ denotes monoclonal
T14491 1966-1971 NN denotes mouse
T14492 1972-1982 NN denotes anti-BMP-6
T14493 1983-1986 CC denotes and
T14494 1987-1997 JJ denotes polyclonal
T14495 1998-2002 NN denotes goat
T14496 2003-2013 NN denotes anti-BMP-6
T14497 2014-2018 IN denotes from
T14498 2019-2020 NNP denotes R
T14499 2021-2022 CC denotes &
T14500 2023-2024 NNP denotes D
T14501 2025-2032 NNP denotes Systems
T14502 2033-2034 -LRB- denotes (
T14503 2034-2042 NNP denotes Abingdon
T14504 2042-2043 -COMMA- denotes ,
T14505 2044-2046 NNP denotes UK
T14506 2046-2047 -RRB- denotes )
T14507 2047-2048 -COMMA- denotes ,
T14508 2049-2052 CC denotes and
T14509 2053-2058 NN denotes mouse
T14510 2059-2069 JJ denotes monoclonal
T14511 2070-2080 JJ denotes anti-BMP-6
T14512 2081-2082 -LRB- denotes (
T14513 2082-2090 NNP denotes Chemicon
T14514 2091-2104 NNP denotes International
T14515 2105-2108 NNP denotes Inc
T14516 2108-2109 -COMMA- denotes ,
T14517 2110-2118 NNP denotes Temecula
T14518 2118-2119 -COMMA- denotes ,
T14519 2120-2122 NNP denotes CA
T14520 2122-2123 -COMMA- denotes ,
T14521 2124-2127 NNP denotes USA
T14522 2127-2128 -RRB- denotes )
T14523 2130-2146 NN denotes Characterisation
T14524 2147-2149 IN denotes of
T14525 2150-2164 JJ denotes BMP-signalling
T14526 2165-2173 NN denotes pathways
T14527 2174-2177 VB denotes was
T14528 2178-2182 VB denotes done
T14529 2183-2185 IN denotes by
T14530 2186-2189 NN denotes use
T14531 2190-2192 IN denotes of
T14532 2193-2211 NN denotes anti-phospho-Smad1
T14533 2211-2212 -COMMA- denotes ,
T14534 2213-2215 CD denotes -5
T14535 2215-2216 -COMMA- denotes ,
T14536 2217-2221 JJ denotes -and
T14537 2222-2223 CD denotes 8
T14538 2224-2234 JJ denotes polyclonal
T14539 2235-2243 NN denotes antibody
T14540 2244-2245 -LRB- denotes (
T14541 2245-2253 NNP denotes Chemicon
T14542 2253-2254 -COMMA- denotes ,
T14543 2255-2263 NNP denotes Temecula
T14544 2263-2264 -COMMA- denotes ,
T14545 2265-2267 NNP denotes CA
T14546 2267-2268 -COMMA- denotes ,
T14547 2269-2272 NNP denotes USA
T14548 2272-2273 -RRB- denotes )
T14549 2275-2285 NN denotes Expression
T14550 2286-2292 NN denotes levels
T14551 2293-2295 IN denotes of
T14552 2296-2301 NN denotes Id1–3
T14553 2302-2310 NN denotes proteins
T14554 2311-2315 VB denotes were
T14555 2316-2324 VB denotes detected
T14556 2325-2329 IN denotes with
T14557 2330-2340 JJ denotes polyclonal
T14558 2341-2347 NN denotes rabbit
T14559 2348-2356 NN denotes antibody
T14560 2357-2360 CC denotes and
T14561 2361-2370 NN denotes detection
T14562 2371-2374 VB denotes was
T14563 2375-2382 VB denotes blocked
T14564 2383-2387 IN denotes with
T14565 2388-2396 VB denotes blocking
T14566 2397-2404 NN denotes peptide
T14567 2405-2409 IN denotes from
T14568 2410-2415 NNP denotes Santa
T14569 2416-2420 NNP denotes Cruz
T14570 2421-2434 NNP denotes Biotechnology
T14571 2435-2436 -LRB- denotes (
T14572 2436-2441 NNP denotes Santa
T14573 2442-2446 NNP denotes Cruz
T14574 2446-2447 -COMMA- denotes ,
T14575 2448-2451 NNP denotes San
T14576 2452-2457 NNP denotes Diego
T14577 2457-2458 -COMMA- denotes ,
T14578 2459-2461 NNP denotes CA
T14579 2461-2462 -COMMA- denotes ,
T14580 2463-2466 NNP denotes USA
T14581 2466-2467 -RRB- denotes )
T14582 2469-2471 IN denotes As
T14583 2472-2481 JJ denotes secondary
T14584 2482-2492 NN denotes antibodies
T14585 2493-2499 VB denotes served
T14586 2500-2510 JJ denotes anti-mouse
T14587 2510-2511 -COMMA- denotes ,
T14588 2512-2521 JJ denotes anti-goat
T14589 2522-2524 CC denotes or
T14590 2525-2536 JJ denotes anti-rabbit
T14591 2537-2541 NN denotes IgG-
T14592 2542-2553 NN denotes horseradish
T14593 2554-2564 NN denotes peroxidase
T14594 2565-2566 -LRB- denotes (
T14595 2566-2569 NN denotes HRP
T14596 2569-2570 -RRB- denotes )
T14597 2571-2575 IN denotes from
T14598 2576-2590 NN denotes Dakocytomation
T14599 2591-2593 NN denotes AS
T14600 2594-2595 -LRB- denotes (
T14601 2595-2605 NN denotes Copenhagen
T14602 2605-2606 -COMMA- denotes ,
T14603 2607-2614 NNP denotes Denmark
T14604 2614-2615 -RRB- denotes )
T14605 2616-2619 IN denotes for
T14606 2620-2630 NN denotes immunoblot
T14607 2631-2639 NN denotes analysis
T14608 2641-2653 NN denotes Anti-β-actin
T14609 2654-2657 VB denotes was
T14610 2658-2662 IN denotes from
T14611 2663-2674 NNP denotes Santa-Cruz.
T14612 2675-2679 NNP denotes From
T14613 2680-2686 NNP denotes Becton
T14614 2687-2696 NNP denotes Dickinson
T14615 2697-2698 -LRB- denotes (
T14616 2698-2701 NNP denotes San
T14617 2702-2706 NNP denotes Jose
T14618 2706-2707 -COMMA- denotes ,
T14619 2708-2710 NNP denotes CA
T14620 2710-2711 -RRB- denotes )
T14621 2711-2712 -COMMA- denotes ,
T14622 2713-2715 PRP denotes we
T14623 2716-2725 VB denotes purchased
T14624 2726-2738 JJ denotes anti-CD19-PE
T14625 2738-2739 -COMMA- denotes ,
T14626 2740-2754 NN denotes anti-CD19-FITC
T14627 2756-2759 DT denotes The
T14628 2760-2770 NN denotes antibodies
T14629 2771-2775 VB denotes used
T14630 2776-2779 IN denotes for
T14631 2780-2784 NN denotes cell
T14632 2785-2792 NN denotes sorting
T14633 2793-2797 VB denotes were
T14634 2798-2807 JJ denotes anti-CD19
T14635 2808-2811 NN denotes PC5
T14636 2812-2816 IN denotes from
T14637 2817-2827 NNP denotes Immunotech
T14638 2828-2830 NNP denotes SA
T14639 2831-2832 -LRB- denotes (
T14640 2832-2841 NNP denotes Marseille
T14641 2841-2842 -COMMA- denotes ,
T14642 2843-2849 NNP denotes France
T14643 2849-2850 -RRB- denotes )
T14644 2851-2854 CC denotes and
T14645 2855-2864 JJ denotes anti-CD27
T14646 2865-2867 NN denotes PE
T14647 2868-2872 IN denotes from
T14648 2873-2879 NNP denotes Becton
T14649 2880-2889 NNP denotes Dickinson
T14650 2889-2890 -COMMA- denotes ,
T14651 2891-2902 NNP denotes Biosciences
T14652 2903-2913 NNP denotes Pharmingen
T14653 2914-2915 -LRB- denotes (
T14654 2915-2918 NNP denotes San
T14655 2919-2924 NNP denotes Diego
T14656 2924-2925 -COMMA- denotes ,
T14657 2926-2928 NNP denotes CA
T14658 2928-2929 -COMMA- denotes ,
T14659 2930-2933 NNP denotes USA
T14660 2933-2934 -RRB- denotes )
T15102 2937-2941 NN denotes Cell
T15103 2942-2949 NN denotes sorting
T15104 2950-2956 RB denotes Highly
T15105 2957-2965 VB denotes purified
T15106 2966-2976 JJ denotes CD19+CD27-
T15107 2977-2979 CC denotes or
T15108 2980-2990 JJ denotes CD19+CD27+
T15109 2991-2996 NN denotes cells
T15110 2997-3001 VB denotes were
T15111 3002-3010 VB denotes obtained
T15112 3011-3013 IN denotes by
T15113 3014-3022 VB denotes staining
T15114 3023-3028 JJ denotes CD19+
T15115 3029-3034 NN denotes cells
T15116 3035-3039 IN denotes with
T15117 3040-3049 JJ denotes anti-CD27
T15118 3050-3052 NN denotes PE
T15119 3053-3056 CC denotes and
T15120 3057-3061 NN denotes CD19
T15121 3062-3065 NN denotes PC5
T15122 3066-3070 NN denotes mAbs
T15123 3071-3074 IN denotes for
T15124 3075-3077 CD denotes 30
T15125 3078-3085 NN denotes minutes
T15126 3086-3088 IN denotes at
T15127 3089-3092 NN denotes 4°C
T15128 3092-3093 -COMMA- denotes ,
T15129 3094-3102 VB denotes followed
T15130 3103-3105 IN denotes by
T15131 3106-3113 VB denotes washing
T15132 3114-3118 IN denotes with
T15133 3119-3122 NNP denotes PBS
T15134 3123-3126 CC denotes and
T15135 3127-3134 VB denotes sorting
T15136 3135-3137 IN denotes on
T15137 3138-3142 NNP denotes FACS
T15138 3143-3147 NNP denotes DiVa
T15139 3148-3152 IN denotes from
T15140 3153-3159 NNP denotes Becton
T15141 3160-3169 NNP denotes Dickinson
T15269 3172-3184 JJ denotes Western-blot
T15270 3185-3193 NN denotes analysis
T15271 3194-3195 NN denotes B
T15272 3196-3201 NN denotes cells
T15273 3202-3206 IN denotes from
T15274 3207-3217 JJ denotes peripheral
T15275 3218-3223 NN denotes blood
T15276 3224-3226 CC denotes or
T15277 3227-3235 VB denotes cultured
T15278 3236-3246 NN denotes cell-lines
T15279 3247-3251 VB denotes were
T15280 3252-3257 VB denotes lysed
T15281 3258-3260 IN denotes in
T15282 3261-3266 NN denotes lysis
T15283 3267-3273 NN denotes buffer
T15284 3274-3275 -LRB- denotes (
T15285 3275-3283 NN denotes glycerol
T15286 3284-3286 CD denotes 10
T15287 3286-3287 NN denotes %
T15288 3287-3288 -COMMA- denotes ,
T15289 3289-3306 NN denotes β-mercaptoethanol
T15290 3307-3308 CD denotes 5
T15291 3308-3309 NN denotes %
T15292 3309-3310 -COMMA- denotes ,
T15293 3311-3317 CD denotes 0.0625
T15294 3318-3319 NN denotes M
T15295 3320-3328 NN denotes Tris-HCL
T15296 3329-3330 -LRB- denotes [
T15297 3330-3332 NN denotes pH
T15298 3333-3336 CD denotes 6.8
T15299 3336-3337 -RRB- denotes ]
T15300 3337-3338 -COMMA- denotes ,
T15301 3339-3345 NN denotes sodium
T15302 3346-3353 NN denotes dodecyl
T15303 3354-3362 NN denotes sulphate
T15304 3363-3364 -LRB- denotes [
T15305 3364-3367 NN denotes SDS
T15306 3367-3368 -RRB- denotes ]
T15307 3369-3372 CD denotes 2.5
T15308 3372-3373 NN denotes %
T15309 3373-3378 NN denotes w/vol
T15310 3378-3379 -RRB- denotes )
T15311 3381-3386 JJ denotes Total
T15312 3387-3394 NN denotes protein
T15313 3395-3396 -LRB- denotes (
T15314 3396-3402 CD denotes 30–100
T15315 3403-3405 NN denotes μg
T15316 3405-3406 -RRB- denotes )
T15317 3407-3411 IN denotes from
T15318 3412-3416 DT denotes each
T15319 3417-3423 NN denotes sample
T15320 3424-3427 VB denotes was
T15321 3428-3431 VB denotes run
T15322 3432-3434 IN denotes on
T15323 3435-3437 CD denotes 10
T15324 3437-3438 NN denotes %
T15325 3439-3441 CC denotes or
T15326 3442-3444 CD denotes 12
T15327 3444-3445 NN denotes %
T15328 3446-3464 NN denotes SDS/polyacrylamide
T15329 3465-3466 -LRB- denotes (
T15330 3466-3474 NN denotes SDS/PAGE
T15331 3474-3475 -RRB- denotes )
T15332 3476-3480 NN denotes gels
T15333 3481-3484 CC denotes and
T15334 3485-3492 VB denotes blotted
T15335 3493-3497 IN denotes onto
T15336 3498-3512 NN denotes nitrocellulose
T15337 3513-3520 NN denotes filters
T15338 3521-3522 -LRB- denotes (
T15339 3522-3529 NNP denotes Protran
T15340 3529-3530 -COLON- denotes ;
T15341 3531-3541 NNP denotes Schleicher
T15342 3542-3543 CC denotes &
T15343 3543-3550 NNP denotes Schuell
T15344 3551-3555 NNP denotes GmbH
T15345 3555-3556 -COMMA- denotes ,
T15346 3557-3563 NNP denotes Dassel
T15347 3563-3564 -COMMA- denotes ,
T15348 3565-3572 NNP denotes Germany
T15349 3572-3573 -RRB- denotes )
T15350 3575-3583 NN denotes Blocking
T15351 3583-3584 -COMMA- denotes ,
T15352 3585-3592 NN denotes washing
T15353 3593-3596 CC denotes and
T15354 3597-3607 NN denotes incubation
T15355 3608-3610 IN denotes of
T15356 3611-3614 DT denotes the
T15357 3615-3622 NN denotes filters
T15358 3623-3627 IN denotes with
T15359 3628-3635 JJ denotes primary
T15360 3636-3646 NN denotes antibodies
T15361 3647-3651 VB denotes were
T15362 3652-3656 VB denotes done
T15363 3657-3666 VB denotes according
T15364 3667-3669 TO denotes to
T15365 3670-3673 DT denotes the
T15366 3674-3686 NN denotes manufacturer
T15367 3686-3688 POS denotes 's
T15368 3689-3698 NN denotes protocols
T15369 3699-3701 IN denotes at
T15370 3702-3706 NN denotes room
T15371 3707-3718 NN denotes temperature
T15372 3719-3720 -LRB- denotes (
T15373 3720-3722 NN denotes RT
T15374 3722-3723 -RRB- denotes )
T15375 3725-3730 IN denotes After
T15376 3731-3738 VB denotes washing
T15377 3739-3743 IN denotes with
T15378 3744-3751 NN denotes TBS/0.1
T15379 3751-3752 NN denotes %
T15380 3753-3761 CD denotes Tween-20
T15381 3762-3763 -LRB- denotes (
T15382 3763-3768 NN denotes TBS-T
T15383 3768-3769 -RRB- denotes )
T15384 3769-3770 -COMMA- denotes ,
T15385 3771-3774 DT denotes the
T15386 3775-3782 NN denotes filters
T15387 3783-3787 VB denotes were
T15388 3788-3797 VB denotes incubated
T15389 3798-3802 IN denotes with
T15390 3803-3814 NN denotes horseradish
T15391 3815-3825 NN denotes peroxidase
T15392 3826-3827 -LRB- denotes (
T15393 3827-3830 NN denotes HRP
T15394 3830-3831 -RRB- denotes )
T15395 3832-3839 VB denotes coupled
T15396 3840-3842 TO denotes to
T15397 3843-3851 JJ denotes relevant
T15398 3852-3861 JJ denotes secondary
T15399 3862-3872 NN denotes antibodies
T15400 3873-3874 -LRB- denotes (
T15401 3874-3877 VB denotes see
T15402 3878-3883 IN denotes above
T15403 3883-3884 -RRB- denotes )
T15404 3885-3888 IN denotes for
T15405 3889-3891 CD denotes 60
T15406 3892-3899 NN denotes minutes
T15407 3900-3902 IN denotes at
T15408 3903-3905 NN denotes RT
T15409 3907-3913 NN denotes Enzyme
T15410 3914-3922 NN denotes activity
T15411 3923-3926 VB denotes was
T15412 3927-3937 VB denotes visualised
T15413 3938-3940 IN denotes by
T15414 3941-3944 DT denotes the
T15415 3945-3953 VB denotes enhanced
T15416 3954-3971 NN denotes chemiluminescence
T15417 3972-3978 NN denotes system
T15418 3978-3979 -COMMA- denotes ,
T15419 3980-3988 NN denotes ECL+PLUS
T15420 3989-3990 -LRB- denotes (
T15421 3990-3998 NNP denotes Amersham
T15422 3998-3999 -COMMA- denotes ,
T15423 4000-4015 NNP denotes Buckinghamshire
T15424 4015-4016 -COMMA- denotes ,
T15425 4017-4019 NNP denotes UK
T15426 4019-4020 -RRB- denotes )
T15427 4022-4035 JJ denotes Densitometric
T15428 4036-4044 NN denotes analysis
T15429 4045-4048 VB denotes was
T15430 4049-4058 VB denotes performed
T15431 4059-4061 IN denotes by
T15432 4062-4070 VB denotes scanning
T15433 4071-4081 NN denotes hyperfilms
T15434 4082-4084 IN denotes on
T15435 4085-4086 DT denotes a
T15436 4087-4095 NNP denotes Personal
T15437 4096-4108 NNP denotes Densitometer
T15438 4109-4111 NNP denotes SI
T15439 4112-4113 -LRB- denotes (
T15440 4113-4122 NNP denotes Molecular
T15441 4123-4131 NNP denotes Dynamics
T15442 4131-4132 -COMMA- denotes ,
T15443 4133-4142 NNP denotes Sunnyvale
T15444 4142-4143 -COMMA- denotes ,
T15445 4144-4146 NNP denotes CA
T15446 4146-4147 -RRB- denotes )
T15447 4149-4163 NN denotes Quantification
T15448 4164-4166 IN denotes of
T15449 4167-4170 NN denotes Id1
T15450 4170-4171 -COMMA- denotes ,
T15451 4172-4175 NN denotes Id2
T15452 4176-4179 CC denotes and
T15453 4180-4183 NN denotes Id3
T15454 4184-4191 NN denotes protein
T15455 4192-4195 VB denotes was
T15456 4196-4206 VB denotes calculated
T15457 4207-4209 IN denotes by
T15458 4210-4221 VB denotes normalizing
T15459 4222-4225 DT denotes the
T15460 4226-4234 JJ denotes specific
T15461 4235-4242 NN denotes protein
T15462 4243-4248 NN denotes bands
T15463 4249-4251 TO denotes to
T15464 4252-4259 NN denotes β-actin
T15465 4260-4265 VB denotes using
T15466 4266-4271 NN denotes Image
T15467 4272-4277 JJ denotes Quant
T15468 4278-4281 CD denotes 5.5
T15469 4282-4290 NN denotes software
T15470 4291-4292 -LRB- denotes (
T15471 4292-4301 NNP denotes Molecular
T15472 4302-4310 NNP denotes Dynamics
T15473 4310-4311 -RRB- denotes )
T15608 4314-4322 NN denotes Analysis
T15609 4323-4325 IN denotes of
T15610 4326-4331 NN denotes BMP-6
T15611 4332-4341 NN denotes messenger
T15612 4342-4345 NN denotes RNA
T15613 4346-4347 -LRB- denotes (
T15614 4347-4351 NN denotes mRNA
T15615 4351-4352 -RRB- denotes )
T15616 4353-4363 NN denotes expression
T15617 4364-4374 JJ denotes Endogenous
T15618 4375-4385 NN denotes expression
T15619 4386-4388 IN denotes of
T15620 4389-4392 DT denotes the
T15621 4393-4398 NN denotes BMP-6
T15622 4399-4403 NN denotes gene
T15623 4404-4407 VB denotes was
T15624 4408-4416 VB denotes examined
T15625 4417-4419 IN denotes by
T15626 4420-4427 JJ denotes reverse
T15627 4428-4452 NN denotes transcription-polymerase
T15628 4453-4458 NN denotes chain
T15629 4459-4467 NN denotes reaction
T15630 4469-4474 JJ denotes Total
T15631 4475-4478 NN denotes RNA
T15632 4479-4482 VB denotes was
T15633 4483-4491 VB denotes isolated
T15634 4492-4497 VB denotes using
T15635 4498-4508 RB denotes Absolutely
T15636 4509-4513 NNP denotes RNA™
T15637 4514-4520 NN denotes RT-PCR
T15638 4521-4529 NNP denotes Miniprep
T15639 4530-4533 NN denotes Kit
T15640 4534-4535 -LRB- denotes (
T15641 4535-4545 NNP denotes Stratagene
T15642 4546-4552 NNP denotes Europe
T15643 4552-4553 -COMMA- denotes ,
T15644 4554-4563 NNP denotes Amsterdam
T15645 4563-4564 -COMMA- denotes ,
T15646 4565-4575 NNP denotes Netherland
T15647 4575-4576 -RRB- denotes )
T15648 4577-4586 VB denotes according
T15649 4587-4589 TO denotes to
T15650 4590-4593 DT denotes the
T15651 4594-4607 NN denotes manufacturers
T15652 4608-4620 NN denotes instructions
T15653 4622-4636 NN denotes Quantification
T15654 4637-4639 IN denotes of
T15655 4640-4643 DT denotes the
T15656 4644-4652 VB denotes isolated
T15657 4653-4658 JJ denotes total
T15658 4659-4662 NN denotes RNA
T15659 4663-4666 VB denotes was
T15660 4667-4675 VB denotes achieved
T15661 4676-4678 IN denotes by
T15662 4679-4684 VB denotes using
T15663 4685-4703 JJ denotes spectrophotometric
T15664 4704-4709 NN denotes OD260
T15665 4710-4722 NN denotes measurements
T15666 4724-4729 JJ denotes Equal
T15667 4730-4737 NN denotes amounts
T15668 4738-4740 IN denotes of
T15669 4741-4744 NN denotes RNA
T15670 4745-4749 VB denotes were
T15671 4750-4754 RB denotes then
T15672 4755-4762 VB denotes reverse
T15673 4763-4774 VB denotes transcribed
T15674 4775-4777 TO denotes to
T15675 4778-4782 NN denotes cDNA
T15676 4783-4787 IN denotes with
T15677 4788-4795 NNP denotes TaqMan®
T15678 4796-4803 NNP denotes Reverse
T15679 4804-4817 NN denotes Transcription
T15680 4818-4826 NN denotes Reagents
T15681 4827-4828 -LRB- denotes (
T15682 4828-4835 NNP denotes Applied
T15683 4836-4846 NNP denotes Biosystems
T15684 4846-4847 -COMMA- denotes ,
T15685 4848-4854 NNP denotes Foster
T15686 4855-4859 NNP denotes City
T15687 4859-4860 -COMMA- denotes ,
T15688 4861-4863 NNP denotes CA
T15689 4863-4864 -COMMA- denotes ,
T15690 4865-4870 NN denotes U.S.A
T15691 4870-4871 -RRB- denotes )
T15692 4873-4875 TO denotes To
T15693 4876-4883 VB denotes measure
T15694 4884-4888 NN denotes mRNA
T15695 4889-4899 NN denotes expression
T15696 4900-4902 IN denotes of
T15697 4903-4907 NN denotes BMP6
T15698 4907-4908 -COMMA- denotes ,
T15699 4909-4916 NN denotes Id1–Id4
T15700 4917-4920 CC denotes and
T15701 4921-4925 NN denotes PGK1
T15702 4926-4929 NN denotes PCR
T15703 4930-4934 VB denotes were
T15704 4935-4942 VB denotes carried
T15705 4943-4946 RP denotes out
T15706 4947-4951 IN denotes with
T15707 4952-4959 NNP denotes TaqMan®
T15708 4960-4969 JJ denotes universal
T15709 4970-4976 NN denotes master
T15710 4977-4980 NN denotes mix
T15711 4982-4989 NN denotes Primers
T15712 4990-4993 CC denotes and
T15713 4994-5000 NN denotes probes
T15714 5001-5005 VB denotes were
T15715 5006-5014 VB denotes provided
T15716 5015-5017 IN denotes by
T15717 5018-5033 NN denotes Assay-on-Demand
T15718 5034-5035 -LRB- denotes (
T15719 5035-5042 NNP denotes Applied
T15720 5043-5053 NNP denotes Biosystems
T15721 5053-5054 -RRB- denotes )
T15722 5056-5059 NN denotes PCR
T15723 5060-5069 NN denotes reactions
T15724 5070-5074 VB denotes were
T15725 5075-5082 VB denotes carried
T15726 5083-5086 RP denotes out
T15727 5087-5089 IN denotes in
T15728 5090-5091 DT denotes a
T15729 5092-5097 JJ denotes final
T15730 5098-5104 NN denotes volume
T15731 5105-5107 IN denotes of
T15732 5108-5110 CD denotes 25
T15733 5111-5113 NN denotes μl
T15734 5114-5115 -LRB- denotes (
T15735 5115-5120 NN denotes BMP-6
T15736 5120-5121 -RRB- denotes )
T15737 5122-5124 CC denotes or
T15738 5125-5127 CD denotes 20
T15739 5128-5130 NN denotes μl
T15740 5131-5132 -LRB- denotes (
T15741 5132-5135 NN denotes ID1
T15742 5135-5136 -RRB- denotes )
T15743 5138-5141 DT denotes The
T15744 5142-5146 NN denotes cDNA
T15745 5147-5152 VB denotes added
T15746 5153-5155 TO denotes to
T15747 5156-5160 DT denotes each
T15748 5161-5169 NN denotes reaction
T15749 5170-5173 VB denotes was
T15750 5174-5184 JJ denotes equivalent
T15751 5185-5187 TO denotes to
T15752 5188-5191 DT denotes the
T15753 5192-5197 NN denotes input
T15754 5198-5200 IN denotes of
T15755 5201-5203 CD denotes 20
T15756 5204-5206 NN denotes ng
T15757 5207-5209 IN denotes of
T15758 5210-5215 JJ denotes total
T15759 5216-5219 NN denotes RNA
T15760 5221-5224 DT denotes The
T15761 5225-5229 NN denotes gene
T15762 5230-5240 NN denotes expression
T15763 5241-5244 VB denotes was
T15764 5245-5255 VB denotes quantified
T15765 5256-5261 VB denotes using
T15766 5262-5265 DT denotes the
T15767 5266-5274 JJ denotes standard
T15768 5275-5280 NN denotes curve
T15769 5281-5287 NN denotes method
T15770 5288-5289 -LRB- denotes (
T15771 5289-5293 NN denotes BMP6
T15772 5293-5294 -RRB- denotes )
T15773 5294-5295 -COMMA- denotes ,
T15774 5296-5298 CC denotes or
T15775 5299-5302 DT denotes the
T15776 5303-5314 JJ denotes comparative
T15777 5315-5317 NN denotes CT
T15778 5318-5324 NN denotes method
T15779 5325-5326 -LRB- denotes (
T15780 5326-5329 NN denotes Id1
T15781 5329-5330 -RRB- denotes )
T15782 5331-5333 IN denotes as
T15783 5334-5343 VB denotes described
T15784 5344-5346 IN denotes in
T15785 5347-5354 NNP denotes ABI7700
T15786 5355-5359 NNP denotes User
T15787 5360-5368 NNP denotes Bulletin
T15788 5369-5370 CD denotes 2
T15789 5371-5372 -LRB- denotes (
T15790 5372-5379 NNP denotes Applied
T15791 5380-5390 NNP denotes Biosystems
T15792 5390-5391 -RRB- denotes )
T15793 5393-5396 DT denotes The
T15794 5397-5407 NN denotes expression
T15795 5408-5411 VB denotes was
T15796 5412-5416 RB denotes then
T15797 5417-5427 VB denotes normalized
T15798 5428-5430 TO denotes to
T15799 5431-5434 DT denotes the
T15800 5435-5445 NN denotes expression
T15801 5446-5451 NN denotes level
T15802 5452-5454 IN denotes of
T15803 5455-5459 NN denotes PGK1
T15804 5461-5465 NN denotes PGK1
T15805 5466-5469 VB denotes was
T15806 5470-5476 VB denotes chosen
T15807 5476-5477 -COMMA- denotes ,
T15808 5478-5485 IN denotes because
T15809 5486-5488 PRP denotes it
T15810 5489-5492 VB denotes has
T15811 5493-5497 VB denotes been
T15812 5498-5503 VB denotes shown
T15813 5504-5506 TO denotes to
T15814 5507-5511 VB denotes have
T15815 5512-5515 JJ denotes low
T15816 5516-5526 NN denotes expression
T15817 5527-5538 NN denotes variability
T15818 5539-5544 IN denotes among
T15819 5545-5555 NN denotes lymphocyte
T15820 5556-5565 NN denotes specimens
T15821 5566-5567 -LRB- denotes [
T15822 5567-5569 CD denotes 56
T15823 5569-5570 -RRB- denotes ]
T15824 5572-5582 NN denotes Expression
T15825 5583-5589 NN denotes levels
T15826 5590-5592 IN denotes in
T15827 5593-5594 NN denotes B
T15828 5595-5600 NN denotes cells
T15829 5601-5605 VB denotes were
T15830 5606-5610 RB denotes then
T15831 5611-5618 VB denotes related
T15832 5619-5621 TO denotes to
T15833 5622-5625 DT denotes the
T15834 5626-5636 NN denotes expression
T15835 5637-5643 NN denotes levels
T15836 5644-5646 IN denotes in
T15837 5647-5652 NN denotes Ramos
T15838 5653-5658 NN denotes cells
T16237 5661-5665 NN denotes Cell
T16238 5666-5679 NN denotes proliferation
T16239 5680-5683 IN denotes For
T16240 5684-5694 NN denotes estimation
T16241 5695-5697 IN denotes of
T16242 5698-5701 NN denotes DNA
T16243 5702-5711 NN denotes synthesis
T16244 5711-5712 -COMMA- denotes ,
T16245 5713-5718 JJ denotes CD19+
T16246 5719-5724 NN denotes cells
T16247 5725-5726 -LRB- denotes (
T16248 5726-5729 CD denotes 7.5
T16249 5730-5731 CC denotes ×
T16250 5732-5735 CD denotes 104
T16251 5736-5745 NN denotes cells/0.2
T16252 5746-5748 NN denotes ml
T16253 5748-5749 -RRB- denotes )
T16254 5750-5752 CC denotes or
T16255 5753-5758 NN denotes Ramos
T16256 5759-5764 NN denotes cells
T16257 5765-5766 -LRB- denotes (
T16258 5766-5767 CD denotes 1
T16259 5768-5769 NN denotes ×
T16260 5770-5773 CD denotes 104
T16261 5774-5783 NN denotes cells/0.2
T16262 5784-5786 NN denotes ml
T16263 5786-5787 -RRB- denotes )
T16264 5788-5792 VB denotes were
T16265 5793-5801 VB denotes cultured
T16266 5802-5804 IN denotes in
T16267 5805-5815 NN denotes triplicate
T16268 5816-5818 IN denotes in
T16269 5819-5829 NN denotes microtiter
T16270 5830-5835 NN denotes wells
T16271 5837-5840 DT denotes The
T16272 5841-5846 NN denotes cells
T16273 5847-5851 VB denotes were
T16274 5852-5858 VB denotes pulsed
T16275 5859-5863 IN denotes with
T16276 5864-5867 CD denotes 3.7
T16277 5868-5869 CC denotes ×
T16278 5870-5875 NN denotes 104Bq
T16279 5876-5877 -LRB- denotes [
T16280 5877-5879 NN denotes 3H
T16281 5879-5880 -RRB- denotes ]
T16282 5880-5889 NN denotes thymidine
T16283 5890-5891 -LRB- denotes (
T16284 5891-5899 NNP denotes Amersham
T16285 5899-5900 -COMMA- denotes ,
T16286 5901-5916 NNP denotes Buckinghamshire
T16287 5916-5917 -COMMA- denotes ,
T16288 5918-5920 NNP denotes UK
T16289 5920-5921 -RRB- denotes )
T16290 5922-5925 IN denotes for
T16291 5926-5929 DT denotes the
T16292 5930-5934 JJ denotes last
T16293 5935-5937 CD denotes 16
T16294 5938-5939 NN denotes h
T16295 5940-5942 IN denotes of
T16296 5943-5944 DT denotes a
T16297 5945-5949 JJ denotes 72-h
T16298 5950-5960 NN denotes incubation
T16299 5962-5965 DT denotes The
T16300 5966-5971 NN denotes cells
T16301 5972-5976 VB denotes were
T16302 5977-5986 VB denotes harvested
T16303 5987-5992 VB denotes using
T16304 5993-5995 DT denotes an
T16305 5996-6005 VB denotes automated
T16306 6006-6010 NN denotes cell
T16307 6011-6020 NN denotes harvester
T16308 6021-6022 -LRB- denotes (
T16309 6022-6029 NNP denotes Packard
T16310 6030-6040 NNP denotes Instrument
T16311 6041-6048 NNP denotes Company
T16312 6048-6049 -COMMA- denotes ,
T16313 6050-6057 NNP denotes Meriden
T16314 6057-6058 -COMMA- denotes ,
T16315 6059-6061 NNP denotes CT
T16316 6061-6062 -COMMA- denotes ,
T16317 6063-6066 NNP denotes USA
T16318 6066-6067 -RRB- denotes )
T16319 6068-6071 CC denotes and
T16320 6072-6073 -LRB- denotes [
T16321 6073-6075 NN denotes 3H
T16322 6075-6076 -RRB- denotes ]
T16323 6076-6085 NN denotes thymidine
T16324 6086-6099 NN denotes incorporation
T16325 6100-6103 VB denotes was
T16326 6104-6114 VB denotes determined
T16327 6115-6117 IN denotes in
T16328 6118-6119 DT denotes a
T16329 6120-6133 NN denotes scintillation
T16330 6134-6141 NN denotes counter
T16331 6142-6143 -LRB- denotes (
T16332 6143-6151 NNP denotes TopCount
T16333 6151-6152 -COMMA- denotes ,
T16334 6153-6160 NNP denotes Packard
T16335 6161-6171 NNP denotes Instrument
T16336 6172-6179 NNP denotes Company
T16337 6180-6184 NNP denotes Inc.
T16338 6184-6185 -COMMA- denotes ,
T16339 6186-6193 NNP denotes Meriden
T16340 6193-6194 -COMMA- denotes ,
T16341 6195-6197 NN denotes CT
T16342 6197-6198 -RRB- denotes )
T16481 6201-6214 NN denotes Determination
T16482 6215-6217 IN denotes of
T16483 6218-6222 NN denotes cell
T16484 6223-6228 NN denotes death
T16485 6229-6233 NN denotes Cell
T16486 6234-6239 NN denotes death
T16487 6240-6243 VB denotes was
T16488 6244-6252 VB denotes measured
T16489 6253-6255 IN denotes by
T16490 6256-6261 JJ denotes vital
T16491 6262-6265 NN denotes dye
T16492 6266-6275 NN denotes exclusion
T16493 6276-6280 NN denotes test
T16494 6281-6283 IN denotes by
T16495 6284-6292 VB denotes staining
T16496 6293-6298 NN denotes cells
T16497 6299-6303 IN denotes with
T16498 6304-6305 CD denotes 5
T16499 6306-6311 NN denotes μg/ml
T16500 6312-6321 NN denotes propidium
T16501 6322-6328 NN denotes iodide
T16502 6329-6330 -LRB- denotes (
T16503 6330-6331 -LRB- denotes [
T16504 6331-6333 NNP denotes PI
T16505 6333-6334 -RRB- denotes ]
T16506 6334-6335 -COLON- denotes ;
T16507 6336-6346 NNP denotes Calbiochem
T16508 6347-6352 NNP denotes Corp.
T16509 6352-6353 -COLON- denotes ;
T16510 6354-6356 NNP denotes La
T16511 6357-6362 NNP denotes Jolla
T16512 6362-6363 -COMMA- denotes ,
T16513 6364-6366 NNP denotes CA
T16514 6366-6367 -COLON- denotes ;
T16515 6368-6369 CD denotes 5
T16516 6370-6375 NN denotes mg/ml
T16517 6375-6376 -RRB- denotes )
T16518 6377-6380 IN denotes for
T16519 6381-6384 CD denotes one
T16520 6385-6391 NN denotes minute
T16521 6392-6394 IN denotes on
T16522 6395-6398 NN denotes ice
T16523 6400-6402 IN denotes At
T16524 6403-6408 JJ denotes least
T16525 6409-6414 CD denotes 1,000
T16526 6415-6420 NN denotes cells
T16527 6421-6424 IN denotes per
T16528 6425-6431 NN denotes sample
T16529 6432-6436 VB denotes were
T16530 6437-6440 VB denotes run
T16531 6441-6443 IN denotes on
T16532 6444-6445 DT denotes a
T16533 6446-6448 NN denotes BD
T16534 6449-6460 NN denotes FACSCalibur
T16535 6461-6465 NN denotes flow
T16536 6466-6475 NN denotes cytometer
T16609 6478-6489 JJ denotes Statistical
T16610 6490-6498 NN denotes analysis
T16611 6499-6502 DT denotes The
T16612 6503-6514 JJ denotes statistical
T16613 6515-6527 NN denotes significance
T16614 6528-6530 IN denotes of
T16615 6531-6542 NN denotes differences
T16616 6543-6550 IN denotes between
T16617 6551-6557 NN denotes groups
T16618 6558-6561 VB denotes was
T16619 6562-6572 VB denotes determined
T16620 6573-6578 VB denotes using
T16621 6579-6582 DT denotes the
T16622 6583-6589 JJ denotes paired
T16623 6590-6600 JJ denotes two-tailed
T16624 6601-6609 NNP denotes Wilcoxon
T16625 6610-6623 JJ denotes nonparametric
T16626 6624-6628 NN denotes test
T16627 6628-6629 -COMMA- denotes ,
T16628 6630-6632 IN denotes by
T16629 6633-6641 VB denotes applying
T16630 6642-6650 NN denotes SPSS10.1
T16631 6651-6659 NN denotes software
T16632 6660-6661 -LRB- denotes (
T16633 6661-6665 NNP denotes SPSS
T16634 6666-6670 NNP denotes Inc.
T16635 6670-6671 -COMMA- denotes ,
T16636 6672-6679 NNP denotes Chicago
T16637 6679-6680 -COMMA- denotes ,
T16638 6681-6683 NNP denotes IL
T16639 6683-6684 -COMMA- denotes ,
T16640 6685-6688 NNP denotes USA
T16641 6688-6689 -RRB- denotes )
T16642 6691-6692 NN denotes P
T16643 6693-6699 NN denotes values
T16644 6700-6704 JJ denotes less
T16645 6705-6709 IN denotes than
T16646 6710-6714 CD denotes 0.05
T16647 6715-6719 VB denotes were
T16648 6720-6730 VB denotes considered
T16649 6731-6742 JJ denotes significant
R10633 T13709 T13708 arg1Of culture,Cell
R10634 T13712 T13710 arg2Of specified,If
R10635 T13712 T13711 arg1Of specified,not
R10636 T13715 T13712 arg2Of cells,specified
R10637 T13715 T13714 arg1Of cells,all
R10638 T13715 T13716 arg1Of cells,were
R10639 T13715 T13717 arg2Of cells,cultured
R10640 T13717 T13710 arg1Of cultured,If
R10641 T13717 T13713 arg1Of cultured,","
R10642 T13717 T13716 arg2Of cultured,were
R10643 T13717 T13718 arg1Of cultured,in
R10644 T13719 T13720 arg1Of X-VIVO,15™
R10645 T13719 T13721 arg1Of X-VIVO,(
R10646 T13722 T13723 arg1Of BioWhittaker,","
R10647 T13723 T13721 arg2Of ",",(
R10648 T13723 T13725 arg1Of ",",","
R10649 T13723 T13726 arg1Of ",",Belgium
R10650 T13724 T13723 arg2Of Verviers,","
R10651 T13727 T13721 arg3Of ),(
R10652 T13729 T13718 arg2Of medium,in
R10653 T13729 T13719 arg1Of medium,X-VIVO
R10654 T13729 T13728 arg1Of medium,serum-free
R10655 T13729 T13730 arg1Of medium,at
R10656 T13731 T13732 arg1Of 37°C,and
R10657 T13732 T13730 arg2Of and,at
R10658 T13732 T13736 arg1Of and,in
R10659 T13733 T13734 arg1Of 5,%
R10660 T13735 T13732 arg2Of CO2,and
R10661 T13735 T13733 arg1Of CO2,5
R10662 T13737 T13736 arg2Of air,in
R10663 T13739 T13738 arg1Of blood,Peripheral
R10664 T13739 T13740 arg1Of blood,was
R10665 T13739 T13741 arg2Of blood,provided
R10666 T13741 T13740 arg2Of provided,was
R10667 T13741 T13750 arg1Of provided,with
R10668 T13745 T13741 arg1Of Bank,provided
R10669 T13745 T13742 arg2Of Bank,by
R10670 T13745 T13743 arg1Of Bank,the
R10671 T13745 T13744 arg1Of Bank,Blood
R10672 T13745 T13746 arg1Of Bank,at
R10673 T13749 T13746 arg2Of Hospital,at
R10674 T13749 T13747 arg1Of Hospital,Buskerud
R10675 T13749 T13748 arg1Of Hospital,Regional
R10676 T13752 T13750 arg2Of agreement,with
R10677 T13752 T13751 arg1Of agreement,formal
R10678 T13752 T13753 arg1Of agreement,by
R10679 T13755 T13757 arg1Of patients,and
R10680 T13757 T13753 arg2Of and,by
R10681 T13757 T13754 arg1Of and,the
R10682 T13757 T13756 arg1Of and,","
R10683 T13757 T13759 arg1Of and,by
R10684 T13758 T13757 arg2Of approval,and
R10685 T13763 T13759 arg2Of committee,by
R10686 T13763 T13760 arg1Of committee,the
R10687 T13763 T13761 arg1Of committee,regional
R10688 T13763 T13762 arg1Of committee,ethics
R10689 T13765 T13764 arg1Of purified,Highly
R10690 T13768 T13765 arg1Of B-lymphocytes,purified
R10691 T13768 T13766 arg1Of B-lymphocytes,resting
R10692 T13768 T13767 arg1Of B-lymphocytes,human
R10693 T13768 T13769 arg1Of B-lymphocytes,(
R10694 T13768 T13773 arg1Of B-lymphocytes,were
R10695 T13768 T13774 arg2Of B-lymphocytes,isolated
R10696 T13768 T13780 arg1Of B-lymphocytes,rosetting
R10697 T13771 T13769 arg2Of cells,(
R10698 T13771 T13770 arg1Of cells,CD19+
R10699 T13772 T13769 arg3Of ),(
R10700 T13774 T13773 arg2Of isolated,were
R10701 T13774 T13775 arg1Of isolated,from
R10702 T13774 T13779 arg1Of isolated,by
R10703 T13778 T13775 arg2Of blood,from
R10704 T13778 T13776 arg1Of blood,the
R10705 T13778 T13777 arg1Of blood,peripheral
R10706 T13780 T13779 arg2Of rosetting,by
R10707 T13780 T13781 arg1Of rosetting,with
R10708 T13780 T13794 arg1Of rosetting,as
R10709 T13780 T13796 arg1Of rosetting,[
R10710 T13783 T13781 arg2Of beads,with
R10711 T13783 T13782 arg1Of beads,immunomagnetic
R10712 T13783 T13784 arg1Of beads,(
R10713 T13786 T13787 arg1Of M450,;
R10714 T13787 T13784 arg2Of ;,(
R10715 T13787 T13785 arg1Of ;,Dynabeads
R10716 T13787 T13791 arg1Of ;,","
R10717 T13787 T13792 arg1Of ;,Norway
R10718 T13788 T13789 arg1Of Dynal,","
R10719 T13789 T13787 arg2Of ",",;
R10720 T13790 T13789 arg2Of Oslo,","
R10721 T13793 T13784 arg3Of ),(
R10722 T13795 T13794 arg2Of described,as
R10723 T13797 T13796 arg2Of 55,[
R10724 T13798 T13796 arg3Of ],[
R10725 T13800 T13799 arg1Of procedure,This
R10726 T13800 T13801 arg1Of procedure,yields
R10727 T13801 T13818 arg1Of yields,as
R10728 T13802 T13803 arg1Of less,than
R10729 T13802 T13805 arg1Of less,%
R10730 T13804 T13803 arg2Of 0.5,than
R10731 T13807 T13802 arg1Of cells,less
R10732 T13807 T13806 arg1Of cells,T
R10733 T13807 T13808 arg1Of cells,","
R10734 T13808 T13814 arg1Of ",",and
R10735 T13809 T13810 arg1Of 0.1,%
R10736 T13812 T13808 arg2Of cells,","
R10737 T13812 T13809 arg1Of cells,0.1
R10738 T13812 T13811 arg1Of cells,NK
R10739 T13814 T13801 arg2Of and,yields
R10740 T13814 T13813 arg1Of and,","
R10741 T13815 T13816 arg1Of 0.5,%
R10742 T13817 T13814 arg2Of monocytes,and
R10743 T13817 T13815 arg1Of monocytes,0.5
R10744 T13819 T13818 arg2Of judged,as
R10745 T13823 T13819 arg1Of staining,judged
R10746 T13823 T13820 arg2Of staining,by
R10747 T13823 T13821 arg1Of staining,indirect
R10748 T13823 T13822 arg1Of staining,immunofluorescence
R10749 T13827 T13824 arg1Of lines,The
R10750 T13827 T13825 arg1Of lines,following
R10751 T13827 T13826 arg1Of lines,cell
R10752 T13827 T13828 arg1Of lines,from
R10753 T13827 T13832 arg1Of lines,were
R10754 T13827 T13833 arg2Of lines,maintained
R10755 T13827 T13870 arg1Of lines,serum-starved
R10756 T13827 T13877 arg2Of lines,cultured
R10757 T13827 T13882 arg1Of lines,included
R10758 T13831 T13828 arg2Of malignancies,from
R10759 T13831 T13829 arg1Of malignancies,human
R10760 T13831 T13830 arg1Of malignancies,lymphoid
R10761 T13833 T13834 arg1Of maintained,in
R10762 T13833 T13869 arg1Of maintained,but
R10763 T13835 T13834 arg2Of RPMI,in
R10764 T13835 T13836 arg1Of RPMI,1640
R10765 T13835 T13837 arg1Of RPMI,(
R10766 T13835 T13846 arg2Of RPMI,supplemented
R10767 T13840 T13837 arg2Of GmbH,(
R10768 T13840 T13838 arg1Of GmbH,PAA
R10769 T13840 T13839 arg1Of GmbH,Laboratories
R10770 T13840 T13841 arg1Of GmbH,","
R10771 T13842 T13841 arg2Of Pasching,","
R10772 T13842 T13843 arg1Of Pasching,","
R10773 T13842 T13844 arg1Of Pasching,Austria
R10774 T13845 T13837 arg3Of ),(
R10775 T13846 T13847 arg1Of supplemented,with
R10776 T13848 T13849 arg1Of 10,%
R10777 T13852 T13848 arg1Of serum,10
R10778 T13852 T13850 arg1Of serum,foetal
R10779 T13852 T13851 arg1Of serum,bovine
R10780 T13852 T13853 arg1Of serum,(
R10781 T13852 T13856 arg1Of serum,","
R10782 T13854 T13853 arg2Of FCS,(
R10783 T13855 T13853 arg3Of ),(
R10784 T13856 T13862 arg1Of ",",and
R10785 T13860 T13856 arg2Of G,","
R10786 T13860 T13857 arg1Of G,100
R10787 T13860 T13858 arg1Of G,units/ml
R10788 T13860 T13859 arg1Of G,penicillin
R10789 T13862 T13847 arg2Of and,with
R10790 T13862 T13861 arg1Of and,","
R10791 T13864 T13862 arg2Of units/ml,and
R10792 T13864 T13863 arg1Of units/ml,100
R10793 T13864 T13865 arg1Of units/ml,of
R10794 T13867 T13865 arg2Of sulphate,of
R10795 T13867 T13866 arg1Of sulphate,streptomycin
R10796 T13869 T13868 arg1Of but,","
R10797 T13869 T13871 arg1Of but,for
R10798 T13869 T13876 arg1Of but,and
R10799 T13870 T13869 arg2Of serum-starved,but
R10800 T13874 T13872 arg1Of four,at
R10801 T13874 T13873 arg1Of four,least
R10802 T13875 T13871 arg2Of hours,for
R10803 T13875 T13874 arg1Of hours,four
R10804 T13876 T13832 arg2Of and,were
R10805 T13876 T13881 arg1Of and,when
R10806 T13877 T13876 arg2Of cultured,and
R10807 T13877 T13878 arg1Of cultured,in
R10808 T13879 T13878 arg2Of X-VIVO,in
R10809 T13879 T13880 arg1Of X-VIVO,15™
R10810 T13882 T13881 arg2Of included,when
R10811 T13882 T13883 arg1Of included,in
R10812 T13884 T13883 arg2Of experiments,in
R10813 T13884 T13885 arg1Of experiments,:
R10814 T13890 T13886 arg1Of Ramos,EBV-negative
R10815 T13890 T13887 arg1Of Ramos,BL
R10816 T13890 T13888 arg1Of Ramos,cell
R10817 T13890 T13889 arg1Of Ramos,lines
R10818 T13890 T13891 arg1Of Ramos,(
R10819 T13890 T13895 arg1Of Ramos,","
R10820 T13892 T13891 arg2Of ECACC,(
R10821 T13892 T13893 arg1Of ECACC,85030802
R10822 T13894 T13891 arg3Of ),(
R10823 T13895 T13882 arg2Of ",",included
R10824 T13896 T13895 arg2Of HL60,","
R10825 T13896 T13897 arg1Of HL60,(
R10826 T13898 T13897 arg2Of JCRB0085,(
R10827 T13899 T13897 arg3Of ),(
R11164 T14428 T14429 arg2Of Antibodies,used
R11165 T14429 T14430 arg1Of used,for
R11166 T14433 T14431 arg1Of analysis,flow
R11167 T14433 T14432 arg1Of analysis,cytometric
R11168 T14433 T14434 arg1Of analysis,and
R11169 T14434 T14430 arg2Of and,for
R11170 T14436 T14434 arg2Of analysis,and
R11171 T14436 T14435 arg1Of analysis,immunoblot
R11172 T14437 T14438 arg1Of Antibodies,against
R11173 T14437 T14451 arg1Of Antibodies,were
R11174 T14437 T14452 arg2Of Antibodies,purchased
R11175 T14441 T14439 arg1Of BMP-receptors,the
R11176 T14441 T14440 arg1Of BMP-receptors,human
R11177 T14442 T14443 arg1Of Act-RIA,","
R11178 T14443 T14445 arg1Of ",",","
R11179 T14444 T14443 arg2Of BMP-RIB,","
R11180 T14445 T14447 arg1Of ",",","
R11181 T14446 T14445 arg2Of BMPR-II,","
R11182 T14447 T14449 arg1Of ",",and
R11183 T14448 T14447 arg2Of Act-RIIA,","
R11184 T14449 T14438 arg2Of and,against
R11185 T14449 T14441 arg1Of and,BMP-receptors
R11186 T14450 T14449 arg2Of Act-RIIb,and
R11187 T14452 T14451 arg2Of purchased,were
R11188 T14452 T14453 arg1Of purchased,from
R11189 T14457 T14453 arg2Of Systems,from
R11190 T14457 T14454 arg1Of Systems,R
R11191 T14457 T14455 arg1Of Systems,&
R11192 T14457 T14456 arg1Of Systems,D
R11193 T14457 T14458 arg1Of Systems,(
R11194 T14459 T14458 arg2Of Abingdon,(
R11195 T14459 T14460 arg1Of Abingdon,","
R11196 T14459 T14461 arg1Of Abingdon,UK
R11197 T14462 T14458 arg3Of ),(
R11198 T14463 T14464 arg1Of Detection,of
R11199 T14463 T14467 arg1Of Detection,has
R11200 T14463 T14468 arg1Of Detection,been
R11201 T14463 T14469 arg2Of Detection,tried
R11202 T14466 T14464 arg2Of BMP-6-protein,of
R11203 T14466 T14465 arg1Of BMP-6-protein,the
R11204 T14469 T14467 arg2Of tried,has
R11205 T14469 T14468 arg2Of tried,been
R11206 T14469 T14470 arg1Of tried,with
R11207 T14473 T14470 arg2Of antibodies,with
R11208 T14473 T14471 arg1Of antibodies,the
R11209 T14473 T14472 arg1Of antibodies,following
R11210 T14473 T14474 arg1Of antibodies,:
R11211 T14477 T14475 arg1Of anti-BMP-6,goat
R11212 T14477 T14476 arg1Of anti-BMP-6,polyclonal
R11213 T14477 T14478 arg1Of anti-BMP-6,(
R11214 T14477 T14489 arg1Of anti-BMP-6,","
R11215 T14480 T14478 arg2Of Cruz,(
R11216 T14480 T14479 arg1Of Cruz,Santa
R11217 T14480 T14481 arg1Of Cruz,","
R11218 T14480 T14486 arg1Of Cruz,","
R11219 T14480 T14487 arg1Of Cruz,USA
R11220 T14483 T14481 arg2Of Diego,","
R11221 T14483 T14482 arg1Of Diego,San
R11222 T14483 T14484 arg1Of Diego,","
R11223 T14485 T14484 arg2Of CA,","
R11224 T14488 T14478 arg3Of ),(
R11225 T14489 T14474 arg2Of ",",:
R11226 T14489 T14497 arg1Of ",",from
R11227 T14492 T14490 arg1Of anti-BMP-6,monoclonal
R11228 T14492 T14491 arg1Of anti-BMP-6,mouse
R11229 T14492 T14493 arg1Of anti-BMP-6,and
R11230 T14493 T14489 arg2Of and,","
R11231 T14496 T14493 arg2Of anti-BMP-6,and
R11232 T14496 T14494 arg1Of anti-BMP-6,polyclonal
R11233 T14496 T14495 arg1Of anti-BMP-6,goat
R11234 T14501 T14498 arg1Of Systems,R
R11235 T14501 T14499 arg1Of Systems,&
R11236 T14501 T14500 arg1Of Systems,D
R11237 T14501 T14502 arg1Of Systems,(
R11238 T14501 T14508 arg1Of Systems,and
R11239 T14503 T14502 arg2Of Abingdon,(
R11240 T14503 T14504 arg1Of Abingdon,","
R11241 T14503 T14505 arg1Of Abingdon,UK
R11242 T14506 T14502 arg3Of ),(
R11243 T14508 T14497 arg2Of and,from
R11244 T14508 T14507 arg1Of and,","
R11245 T14511 T14508 arg2Of anti-BMP-6,and
R11246 T14511 T14509 arg1Of anti-BMP-6,mouse
R11247 T14511 T14510 arg1Of anti-BMP-6,monoclonal
R11248 T14511 T14512 arg1Of anti-BMP-6,(
R11249 T14515 T14512 arg2Of Inc,(
R11250 T14515 T14513 arg1Of Inc,Chemicon
R11251 T14515 T14514 arg1Of Inc,International
R11252 T14515 T14516 arg1Of Inc,","
R11253 T14515 T14520 arg1Of Inc,","
R11254 T14515 T14521 arg1Of Inc,USA
R11255 T14517 T14518 arg1Of Temecula,","
R11256 T14518 T14516 arg2Of ",",","
R11257 T14519 T14518 arg2Of CA,","
R11258 T14522 T14512 arg3Of ),(
R11259 T14523 T14524 arg1Of Characterisation,of
R11260 T14523 T14527 arg1Of Characterisation,was
R11261 T14523 T14528 arg2Of Characterisation,done
R11262 T14526 T14524 arg2Of pathways,of
R11263 T14526 T14525 arg1Of pathways,BMP-signalling
R11264 T14528 T14527 arg2Of done,was
R11265 T14528 T14540 arg1Of done,(
R11266 T14530 T14528 arg1Of use,done
R11267 T14530 T14529 arg2Of use,by
R11268 T14530 T14531 arg1Of use,of
R11269 T14532 T14531 arg2Of anti-phospho-Smad1,of
R11270 T14532 T14533 arg1Of anti-phospho-Smad1,","
R11271 T14532 T14535 arg1Of anti-phospho-Smad1,","
R11272 T14534 T14533 arg2Of -5,","
R11273 T14538 T14536 arg1Of polyclonal,-and
R11274 T14538 T14537 arg1Of polyclonal,8
R11275 T14539 T14535 arg2Of antibody,","
R11276 T14539 T14538 arg1Of antibody,polyclonal
R11277 T14541 T14542 arg1Of Chemicon,","
R11278 T14542 T14544 arg1Of ",",","
R11279 T14543 T14542 arg2Of Temecula,","
R11280 T14544 T14540 arg2Of ",",(
R11281 T14544 T14546 arg1Of ",",","
R11282 T14544 T14547 arg1Of ",",USA
R11283 T14545 T14544 arg2Of CA,","
R11284 T14548 T14540 arg3Of ),(
R11285 T14550 T14549 arg1Of levels,Expression
R11286 T14550 T14551 arg1Of levels,of
R11287 T14550 T14554 arg1Of levels,were
R11288 T14550 T14555 arg2Of levels,detected
R11289 T14553 T14551 arg2Of proteins,of
R11290 T14553 T14552 arg1Of proteins,Id1–3
R11291 T14555 T14554 arg2Of detected,were
R11292 T14555 T14556 arg1Of detected,with
R11293 T14555 T14560 arg1Of detected,and
R11294 T14559 T14556 arg2Of antibody,with
R11295 T14559 T14557 arg1Of antibody,polyclonal
R11296 T14559 T14558 arg1Of antibody,rabbit
R11297 T14561 T14562 arg1Of detection,was
R11298 T14561 T14563 arg2Of detection,blocked
R11299 T14563 T14560 arg2Of blocked,and
R11300 T14563 T14562 arg2Of blocked,was
R11301 T14563 T14564 arg1Of blocked,with
R11302 T14565 T14564 arg2Of blocking,with
R11303 T14566 T14565 arg2Of peptide,blocking
R11304 T14566 T14567 arg1Of peptide,from
R11305 T14570 T14567 arg2Of Biotechnology,from
R11306 T14570 T14568 arg1Of Biotechnology,Santa
R11307 T14570 T14569 arg1Of Biotechnology,Cruz
R11308 T14570 T14571 arg1Of Biotechnology,(
R11309 T14573 T14571 arg2Of Cruz,(
R11310 T14573 T14572 arg1Of Cruz,Santa
R11311 T14573 T14574 arg1Of Cruz,","
R11312 T14573 T14579 arg1Of Cruz,","
R11313 T14576 T14574 arg2Of Diego,","
R11314 T14576 T14575 arg1Of Diego,San
R11315 T14576 T14577 arg1Of Diego,","
R11316 T14578 T14577 arg2Of CA,","
R11317 T14580 T14579 arg2Of USA,","
R11318 T14581 T14571 arg3Of ),(
R11319 T14584 T14582 arg1Of antibodies,As
R11320 T14584 T14583 arg1Of antibodies,secondary
R11321 T14584 T14585 arg1Of antibodies,served
R11322 T14586 T14587 arg1Of anti-mouse,","
R11323 T14587 T14589 arg1Of ",",or
R11324 T14588 T14587 arg2Of anti-goat,","
R11325 T14590 T14589 arg2Of anti-rabbit,or
R11326 T14593 T14585 arg2Of peroxidase,served
R11327 T14593 T14586 arg1Of peroxidase,anti-mouse
R11328 T14593 T14588 arg1Of peroxidase,anti-goat
R11329 T14593 T14590 arg1Of peroxidase,anti-rabbit
R11330 T14593 T14591 arg1Of peroxidase,IgG-
R11331 T14593 T14592 arg1Of peroxidase,horseradish
R11332 T14593 T14594 arg1Of peroxidase,(
R11333 T14593 T14597 arg1Of peroxidase,from
R11334 T14593 T14600 arg1Of peroxidase,(
R11335 T14593 T14605 arg1Of peroxidase,for
R11336 T14595 T14594 arg2Of HRP,(
R11337 T14596 T14594 arg3Of ),(
R11338 T14599 T14597 arg2Of AS,from
R11339 T14599 T14598 arg1Of AS,Dakocytomation
R11340 T14601 T14600 arg2Of Copenhagen,(
R11341 T14601 T14602 arg1Of Copenhagen,","
R11342 T14603 T14602 arg2Of Denmark,","
R11343 T14604 T14600 arg3Of ),(
R11344 T14607 T14605 arg2Of analysis,for
R11345 T14607 T14606 arg1Of analysis,immunoblot
R11346 T14608 T14609 arg1Of Anti-β-actin,was
R11347 T14608 T14610 arg1Of Anti-β-actin,from
R11348 T14609 T14626 arg1Of was,anti-CD19-FITC
R11349 T14610 T14609 arg2Of from,was
R11350 T14614 T14610 arg2Of Dickinson,from
R11351 T14614 T14611 arg1Of Dickinson,Santa-Cruz.
R11352 T14614 T14612 arg1Of Dickinson,From
R11353 T14614 T14613 arg1Of Dickinson,Becton
R11354 T14614 T14615 arg1Of Dickinson,(
R11355 T14614 T14623 arg2Of Dickinson,purchased
R11356 T14617 T14615 arg2Of Jose,(
R11357 T14617 T14616 arg1Of Jose,San
R11358 T14617 T14618 arg1Of Jose,","
R11359 T14619 T14618 arg2Of CA,","
R11360 T14620 T14615 arg3Of ),(
R11361 T14622 T14623 arg1Of we,purchased
R11362 T14623 T14621 arg1Of purchased,","
R11363 T14626 T14624 arg1Of anti-CD19-FITC,anti-CD19-PE
R11364 T14626 T14625 arg1Of anti-CD19-FITC,","
R11365 T14628 T14627 arg1Of antibodies,The
R11366 T14628 T14629 arg2Of antibodies,used
R11367 T14628 T14633 arg1Of antibodies,were
R11368 T14629 T14630 arg1Of used,for
R11369 T14632 T14630 arg2Of sorting,for
R11370 T14632 T14631 arg1Of sorting,cell
R11371 T14635 T14634 arg1Of PC5,anti-CD19
R11372 T14635 T14636 arg1Of PC5,from
R11373 T14635 T14639 arg1Of PC5,(
R11374 T14635 T14644 arg1Of PC5,and
R11375 T14638 T14636 arg2Of SA,from
R11376 T14638 T14637 arg1Of SA,Immunotech
R11377 T14640 T14639 arg2Of Marseille,(
R11378 T14640 T14641 arg1Of Marseille,","
R11379 T14642 T14641 arg2Of France,","
R11380 T14643 T14639 arg3Of ),(
R11381 T14644 T14633 arg2Of and,were
R11382 T14646 T14644 arg2Of PE,and
R11383 T14646 T14645 arg1Of PE,anti-CD27
R11384 T14646 T14647 arg1Of PE,from
R11385 T14649 T14647 arg2Of Dickinson,from
R11386 T14649 T14648 arg1Of Dickinson,Becton
R11387 T14649 T14650 arg1Of Dickinson,","
R11388 T14652 T14650 arg2Of Pharmingen,","
R11389 T14652 T14651 arg1Of Pharmingen,Biosciences
R11390 T14652 T14653 arg1Of Pharmingen,(
R11391 T14655 T14653 arg2Of Diego,(
R11392 T14655 T14654 arg1Of Diego,San
R11393 T14655 T14656 arg1Of Diego,","
R11394 T14657 T14656 arg2Of CA,","
R11395 T14657 T14658 arg1Of CA,","
R11396 T14659 T14658 arg2Of USA,","
R11397 T14660 T14653 arg3Of ),(
R11658 T15103 T15102 arg1Of sorting,Cell
R11659 T15105 T15104 arg1Of purified,Highly
R11660 T15106 T15107 arg1Of CD19+CD27-,or
R11661 T15108 T15107 arg2Of CD19+CD27+,or
R11662 T15109 T15105 arg1Of cells,purified
R11663 T15109 T15106 arg1Of cells,CD19+CD27-
R11664 T15109 T15108 arg1Of cells,CD19+CD27+
R11665 T15109 T15110 arg1Of cells,were
R11666 T15109 T15111 arg2Of cells,obtained
R11667 T15111 T15110 arg2Of obtained,were
R11668 T15111 T15112 arg1Of obtained,by
R11669 T15111 T15128 arg1Of obtained,","
R11670 T15111 T15129 modOf obtained,followed
R11671 T15113 T15112 arg2Of staining,by
R11672 T15113 T15116 arg1Of staining,with
R11673 T15115 T15113 arg2Of cells,staining
R11674 T15115 T15114 arg1Of cells,CD19+
R11675 T15118 T15119 arg1Of PE,and
R11676 T15120 T15119 arg2Of CD19,and
R11677 T15122 T15116 arg2Of mAbs,with
R11678 T15122 T15117 arg1Of mAbs,anti-CD27
R11679 T15122 T15118 arg1Of mAbs,PE
R11680 T15122 T15120 arg1Of mAbs,CD19
R11681 T15122 T15121 arg1Of mAbs,PC5
R11682 T15122 T15123 arg1Of mAbs,for
R11683 T15125 T15123 arg2Of minutes,for
R11684 T15125 T15124 arg1Of minutes,30
R11685 T15125 T15126 arg1Of minutes,at
R11686 T15127 T15126 arg2Of 4°C,at
R11687 T15129 T15130 arg1Of followed,by
R11688 T15131 T15132 arg1Of washing,with
R11689 T15131 T15134 arg1Of washing,and
R11690 T15133 T15132 arg2Of PBS,with
R11691 T15134 T15130 arg2Of and,by
R11692 T15135 T15134 arg2Of sorting,and
R11693 T15135 T15136 arg1Of sorting,on
R11694 T15138 T15136 arg2Of DiVa,on
R11695 T15138 T15137 arg1Of DiVa,FACS
R11696 T15138 T15139 arg1Of DiVa,from
R11697 T15141 T15139 arg2Of Dickinson,from
R11698 T15141 T15140 arg1Of Dickinson,Becton
R11752 T15270 T15269 arg1Of analysis,Western-blot
R11753 T15272 T15271 arg1Of cells,B
R11754 T15272 T15273 arg1Of cells,from
R11755 T15272 T15279 arg1Of cells,were
R11756 T15272 T15280 arg2Of cells,lysed
R11757 T15275 T15274 arg1Of blood,peripheral
R11758 T15275 T15276 arg1Of blood,or
R11759 T15276 T15273 arg2Of or,from
R11760 T15278 T15276 arg2Of cell-lines,or
R11761 T15278 T15277 arg2Of cell-lines,cultured
R11762 T15280 T15279 arg2Of lysed,were
R11763 T15280 T15281 arg1Of lysed,in
R11764 T15283 T15281 arg2Of buffer,in
R11765 T15283 T15282 arg1Of buffer,lysis
R11766 T15283 T15284 arg1Of buffer,(
R11767 T15287 T15285 arg1Of %,glycerol
R11768 T15287 T15286 arg1Of %,10
R11769 T15287 T15288 arg1Of %,","
R11770 T15288 T15292 arg1Of ",",","
R11771 T15291 T15288 arg2Of %,","
R11772 T15291 T15289 arg1Of %,β-mercaptoethanol
R11773 T15291 T15290 arg1Of %,5
R11774 T15292 T15300 arg1Of ",",","
R11775 T15293 T15294 arg1Of 0.0625,M
R11776 T15295 T15292 arg2Of Tris-HCL,","
R11777 T15295 T15293 arg1Of Tris-HCL,0.0625
R11778 T15295 T15296 arg1Of Tris-HCL,[
R11779 T15297 T15296 arg2Of pH,[
R11780 T15297 T15298 arg1Of pH,6.8
R11781 T15299 T15296 arg3Of ],[
R11782 T15300 T15284 arg2Of ",",(
R11783 T15305 T15304 arg2Of SDS,[
R11784 T15306 T15304 arg3Of ],[
R11785 T15307 T15308 arg1Of 2.5,%
R11786 T15309 T15300 arg2Of w/vol,","
R11787 T15309 T15301 arg1Of w/vol,sodium
R11788 T15309 T15302 arg1Of w/vol,dodecyl
R11789 T15309 T15303 arg1Of w/vol,sulphate
R11790 T15309 T15304 arg1Of w/vol,[
R11791 T15309 T15307 arg1Of w/vol,2.5
R11792 T15310 T15284 arg3Of ),(
R11793 T15312 T15311 arg1Of protein,Total
R11794 T15312 T15313 arg1Of protein,(
R11795 T15312 T15317 arg1Of protein,from
R11796 T15312 T15320 arg1Of protein,was
R11797 T15312 T15321 arg2Of protein,run
R11798 T15312 T15334 arg1Of protein,blotted
R11799 T15315 T15313 arg2Of μg,(
R11800 T15315 T15314 arg1Of μg,30–100
R11801 T15316 T15313 arg3Of ),(
R11802 T15319 T15317 arg2Of sample,from
R11803 T15319 T15318 arg1Of sample,each
R11804 T15321 T15320 arg2Of run,was
R11805 T15321 T15322 arg1Of run,on
R11806 T15321 T15333 arg1Of run,and
R11807 T15324 T15323 arg1Of %,10
R11808 T15324 T15325 arg1Of %,or
R11809 T15325 T15327 arg1Of or,%
R11810 T15326 T15325 arg2Of 12,or
R11811 T15330 T15329 arg2Of SDS/PAGE,(
R11812 T15331 T15329 arg3Of ),(
R11813 T15332 T15322 arg2Of gels,on
R11814 T15332 T15324 arg1Of gels,%
R11815 T15332 T15326 arg1Of gels,12
R11816 T15332 T15328 arg1Of gels,SDS/polyacrylamide
R11817 T15332 T15329 arg1Of gels,(
R11818 T15333 T15338 arg1Of and,(
R11819 T15334 T15333 arg2Of blotted,and
R11820 T15334 T15335 arg1Of blotted,onto
R11821 T15337 T15335 arg2Of filters,onto
R11822 T15337 T15336 arg1Of filters,nitrocellulose
R11823 T15339 T15340 arg1Of Protran,;
R11824 T15340 T15338 arg2Of ;,(
R11825 T15344 T15340 arg2Of GmbH,;
R11826 T15344 T15341 arg1Of GmbH,Schleicher
R11827 T15344 T15342 arg1Of GmbH,&
R11828 T15344 T15343 arg1Of GmbH,Schuell
R11829 T15344 T15345 arg1Of GmbH,","
R11830 T15346 T15345 arg2Of Dassel,","
R11831 T15346 T15347 arg1Of Dassel,","
R11832 T15348 T15347 arg2Of Germany,","
R11833 T15349 T15338 arg3Of ),(
R11834 T15350 T15351 arg1Of Blocking,","
R11835 T15351 T15353 arg1Of ",",and
R11836 T15352 T15351 arg2Of washing,","
R11837 T15353 T15355 arg1Of and,of
R11838 T15353 T15361 arg1Of and,were
R11839 T15353 T15362 arg2Of and,done
R11840 T15354 T15353 arg2Of incubation,and
R11841 T15357 T15355 arg2Of filters,of
R11842 T15357 T15356 arg1Of filters,the
R11843 T15357 T15358 arg1Of filters,with
R11844 T15360 T15358 arg2Of antibodies,with
R11845 T15360 T15359 arg1Of antibodies,primary
R11846 T15362 T15361 arg2Of done,were
R11847 T15362 T15363 arg1Of done,according
R11848 T15362 T15369 arg1Of done,at
R11849 T15364 T15363 arg2Of to,according
R11850 T15366 T15365 arg1Of manufacturer,the
R11851 T15366 T15367 arg2Of manufacturer,'s
R11852 T15368 T15364 arg2Of protocols,to
R11853 T15368 T15367 arg1Of protocols,'s
R11854 T15371 T15369 arg2Of temperature,at
R11855 T15371 T15370 arg1Of temperature,room
R11856 T15371 T15372 arg1Of temperature,(
R11857 T15373 T15372 arg2Of RT,(
R11858 T15374 T15372 arg3Of ),(
R11859 T15376 T15375 arg2Of washing,After
R11860 T15376 T15377 arg1Of washing,with
R11861 T15379 T15377 arg2Of %,with
R11862 T15379 T15378 arg1Of %,TBS/0.1
R11863 T15379 T15380 arg1Of %,Tween-20
R11864 T15379 T15381 arg1Of %,(
R11865 T15382 T15381 arg2Of TBS-T,(
R11866 T15383 T15381 arg3Of ),(
R11867 T15386 T15385 arg1Of filters,the
R11868 T15386 T15387 arg1Of filters,were
R11869 T15386 T15388 arg2Of filters,incubated
R11870 T15388 T15376 arg2Of incubated,washing
R11871 T15388 T15384 arg1Of incubated,","
R11872 T15388 T15387 arg2Of incubated,were
R11873 T15388 T15389 arg1Of incubated,with
R11874 T15391 T15389 arg2Of peroxidase,with
R11875 T15391 T15390 arg1Of peroxidase,horseradish
R11876 T15391 T15392 arg1Of peroxidase,(
R11877 T15391 T15395 arg2Of peroxidase,coupled
R11878 T15393 T15392 arg2Of HRP,(
R11879 T15394 T15392 arg3Of ),(
R11880 T15395 T15396 arg1Of coupled,to
R11881 T15399 T15396 arg2Of antibodies,to
R11882 T15399 T15397 arg1Of antibodies,relevant
R11883 T15399 T15398 arg1Of antibodies,secondary
R11884 T15401 T15375 arg1Of see,After
R11885 T15401 T15400 arg1Of see,(
R11886 T15401 T15402 arg1Of see,above
R11887 T15401 T15403 arg1Of see,)
R11888 T15401 T15404 arg1Of see,for
R11889 T15406 T15404 arg2Of minutes,for
R11890 T15406 T15405 arg1Of minutes,60
R11891 T15406 T15407 arg1Of minutes,at
R11892 T15408 T15401 arg2Of RT,see
R11893 T15410 T15409 arg1Of activity,Enzyme
R11894 T15410 T15411 arg1Of activity,was
R11895 T15410 T15412 arg2Of activity,visualised
R11896 T15412 T15411 arg2Of visualised,was
R11897 T15412 T15420 arg1Of visualised,(
R11898 T15417 T15412 arg1Of system,visualised
R11899 T15417 T15413 arg2Of system,by
R11900 T15417 T15414 arg1Of system,the
R11901 T15417 T15415 arg2Of system,enhanced
R11902 T15417 T15416 arg1Of system,chemiluminescence
R11903 T15417 T15418 arg1Of system,","
R11904 T15419 T15418 arg2Of ECL+PLUS,","
R11905 T15421 T15422 arg1Of Amersham,","
R11906 T15422 T15420 arg2Of ",",(
R11907 T15422 T15424 arg1Of ",",","
R11908 T15423 T15422 arg2Of Buckinghamshire,","
R11909 T15425 T15424 arg2Of UK,","
R11910 T15426 T15420 arg3Of ),(
R11911 T15428 T15427 arg1Of analysis,Densitometric
R11912 T15428 T15429 arg1Of analysis,was
R11913 T15428 T15430 arg2Of analysis,performed
R11914 T15430 T15429 arg2Of performed,was
R11915 T15433 T15430 arg1Of hyperfilms,performed
R11916 T15433 T15431 arg2Of hyperfilms,by
R11917 T15433 T15432 arg1Of hyperfilms,scanning
R11918 T15433 T15434 arg1Of hyperfilms,on
R11919 T15438 T15434 arg2Of SI,on
R11920 T15438 T15435 arg1Of SI,a
R11921 T15438 T15436 arg1Of SI,Personal
R11922 T15438 T15437 arg1Of SI,Densitometer
R11923 T15438 T15439 arg1Of SI,(
R11924 T15441 T15439 arg2Of Dynamics,(
R11925 T15441 T15440 arg1Of Dynamics,Molecular
R11926 T15441 T15442 arg1Of Dynamics,","
R11927 T15443 T15442 arg2Of Sunnyvale,","
R11928 T15443 T15444 arg1Of Sunnyvale,","
R11929 T15445 T15444 arg2Of CA,","
R11930 T15446 T15439 arg3Of ),(
R11931 T15447 T15448 arg1Of Quantification,of
R11932 T15447 T15455 arg1Of Quantification,was
R11933 T15447 T15456 arg2Of Quantification,calculated
R11934 T15449 T15450 arg1Of Id1,","
R11935 T15450 T15452 arg1Of ",",and
R11936 T15451 T15450 arg2Of Id2,","
R11937 T15453 T15452 arg2Of Id3,and
R11938 T15454 T15448 arg2Of protein,of
R11939 T15454 T15449 arg1Of protein,Id1
R11940 T15454 T15451 arg1Of protein,Id2
R11941 T15454 T15453 arg1Of protein,Id3
R11942 T15456 T15455 arg2Of calculated,was
R11943 T15456 T15457 arg1Of calculated,by
R11944 T15458 T15457 arg2Of normalizing,by
R11945 T15462 T15458 arg2Of bands,normalizing
R11946 T15462 T15459 arg1Of bands,the
R11947 T15462 T15460 arg1Of bands,specific
R11948 T15462 T15461 arg1Of bands,protein
R11949 T15462 T15463 arg1Of bands,to
R11950 T15462 T15465 arg1Of bands,using
R11951 T15464 T15463 arg2Of β-actin,to
R11952 T15469 T15465 arg2Of software,using
R11953 T15469 T15466 arg1Of software,Image
R11954 T15469 T15467 arg1Of software,Quant
R11955 T15469 T15468 arg1Of software,5.5
R11956 T15469 T15470 arg1Of software,(
R11957 T15472 T15470 arg2Of Dynamics,(
R11958 T15472 T15471 arg1Of Dynamics,Molecular
R11959 T15473 T15470 arg3Of ),(
R11992 T15608 T15609 arg1Of Analysis,of
R11993 T15612 T15609 arg2Of RNA,of
R11994 T15612 T15610 arg1Of RNA,BMP-6
R11995 T15612 T15611 arg1Of RNA,messenger
R11996 T15612 T15613 arg1Of RNA,(
R11997 T15614 T15613 arg2Of mRNA,(
R11998 T15615 T15613 arg3Of ),(
R11999 T15616 T15608 arg1Of expression,Analysis
R12000 T15618 T15617 arg1Of expression,Endogenous
R12001 T15618 T15619 arg1Of expression,of
R12002 T15618 T15623 arg1Of expression,was
R12003 T15618 T15624 arg2Of expression,examined
R12004 T15622 T15619 arg2Of gene,of
R12005 T15622 T15620 arg1Of gene,the
R12006 T15622 T15621 arg1Of gene,BMP-6
R12007 T15624 T15623 arg2Of examined,was
R12008 T15629 T15624 arg1Of reaction,examined
R12009 T15629 T15625 arg2Of reaction,by
R12010 T15629 T15626 arg1Of reaction,reverse
R12011 T15629 T15627 arg1Of reaction,transcription-polymerase
R12012 T15629 T15628 arg1Of reaction,chain
R12013 T15631 T15630 arg1Of RNA,Total
R12014 T15631 T15632 arg1Of RNA,was
R12015 T15631 T15633 arg2Of RNA,isolated
R12016 T15633 T15632 arg2Of isolated,was
R12017 T15634 T15633 arg3Of using,isolated
R12018 T15634 T15635 arg1Of using,Absolutely
R12019 T15634 T15648 arg1Of using,according
R12020 T15638 T15636 arg1Of Miniprep,RNA™
R12021 T15638 T15637 arg1Of Miniprep,RT-PCR
R12022 T15639 T15634 arg2Of Kit,using
R12023 T15639 T15638 arg1Of Kit,Miniprep
R12024 T15639 T15640 arg1Of Kit,(
R12025 T15642 T15641 arg1Of Europe,Stratagene
R12026 T15642 T15643 arg1Of Europe,","
R12027 T15643 T15640 arg2Of ",",(
R12028 T15643 T15645 arg1Of ",",","
R12029 T15643 T15646 arg1Of ",",Netherland
R12030 T15644 T15643 arg2Of Amsterdam,","
R12031 T15647 T15640 arg3Of ),(
R12032 T15649 T15648 arg2Of to,according
R12033 T15652 T15649 arg2Of instructions,to
R12034 T15652 T15650 arg1Of instructions,the
R12035 T15652 T15651 arg1Of instructions,manufacturers
R12036 T15653 T15654 arg1Of Quantification,of
R12037 T15653 T15659 arg1Of Quantification,was
R12038 T15653 T15660 arg2Of Quantification,achieved
R12039 T15658 T15654 arg2Of RNA,of
R12040 T15658 T15655 arg1Of RNA,the
R12041 T15658 T15656 arg2Of RNA,isolated
R12042 T15658 T15657 arg1Of RNA,total
R12043 T15660 T15659 arg2Of achieved,was
R12044 T15660 T15661 arg1Of achieved,by
R12045 T15662 T15661 arg2Of using,by
R12046 T15665 T15662 arg2Of measurements,using
R12047 T15665 T15663 arg1Of measurements,spectrophotometric
R12048 T15665 T15664 arg1Of measurements,OD260
R12049 T15667 T15666 arg1Of amounts,Equal
R12050 T15667 T15668 arg1Of amounts,of
R12051 T15667 T15670 arg1Of amounts,were
R12052 T15669 T15668 arg2Of RNA,of
R12053 T15670 T15671 arg1Of were,then
R12054 T15672 T15670 arg2Of reverse,were
R12055 T15672 T15673 modOf reverse,transcribed
R12056 T15673 T15674 arg1Of transcribed,to
R12057 T15675 T15674 arg2Of cDNA,to
R12058 T15675 T15676 arg1Of cDNA,with
R12059 T15678 T15677 arg1Of Reverse,TaqMan®
R12060 T15680 T15676 arg2Of Reagents,with
R12061 T15680 T15678 arg1Of Reagents,Reverse
R12062 T15680 T15679 arg1Of Reagents,Transcription
R12063 T15680 T15681 arg1Of Reagents,(
R12064 T15683 T15682 arg1Of Biosystems,Applied
R12065 T15683 T15684 arg1Of Biosystems,","
R12066 T15684 T15687 arg1Of ",",","
R12067 T15686 T15684 arg2Of City,","
R12068 T15686 T15685 arg1Of City,Foster
R12069 T15687 T15681 arg2Of ",",(
R12070 T15687 T15689 arg1Of ",",","
R12071 T15688 T15687 arg2Of CA,","
R12072 T15690 T15689 arg2Of U.S.A,","
R12073 T15691 T15681 arg3Of ),(
R12074 T15693 T15692 arg1Of measure,To
R12075 T15695 T15693 arg2Of expression,measure
R12076 T15695 T15694 arg1Of expression,mRNA
R12077 T15695 T15696 arg1Of expression,of
R12078 T15697 T15696 arg2Of BMP6,of
R12079 T15699 T15700 arg1Of Id1–Id4,and
R12080 T15701 T15700 arg2Of PGK1,and
R12081 T15702 T15693 arg1Of PCR,measure
R12082 T15702 T15699 arg1Of PCR,Id1–Id4
R12083 T15702 T15701 arg1Of PCR,PGK1
R12084 T15702 T15703 arg1Of PCR,were
R12085 T15702 T15704 arg2Of PCR,carried
R12086 T15704 T15692 modOf carried,To
R12087 T15704 T15698 arg1Of carried,","
R12088 T15704 T15703 arg2Of carried,were
R12089 T15704 T15705 arg1Of carried,out
R12090 T15704 T15706 arg1Of carried,with
R12091 T15710 T15706 arg2Of mix,with
R12092 T15710 T15707 arg1Of mix,TaqMan®
R12093 T15710 T15708 arg1Of mix,universal
R12094 T15710 T15709 arg1Of mix,master
R12095 T15711 T15712 arg1Of Primers,and
R12096 T15712 T15714 arg1Of and,were
R12097 T15712 T15715 arg2Of and,provided
R12098 T15713 T15712 arg2Of probes,and
R12099 T15715 T15714 arg2Of provided,were
R12100 T15717 T15715 arg1Of Assay-on-Demand,provided
R12101 T15717 T15716 arg2Of Assay-on-Demand,by
R12102 T15717 T15718 arg1Of Assay-on-Demand,(
R12103 T15720 T15718 arg2Of Biosystems,(
R12104 T15720 T15719 arg1Of Biosystems,Applied
R12105 T15721 T15718 arg3Of ),(
R12106 T15723 T15722 arg1Of reactions,PCR
R12107 T15723 T15724 arg1Of reactions,were
R12108 T15723 T15725 arg2Of reactions,carried
R12109 T15725 T15724 arg2Of carried,were
R12110 T15725 T15726 arg1Of carried,out
R12111 T15725 T15727 arg1Of carried,in
R12112 T15730 T15727 arg2Of volume,in
R12113 T15730 T15728 arg1Of volume,a
R12114 T15730 T15729 arg1Of volume,final
R12115 T15730 T15731 arg1Of volume,of
R12116 T15733 T15732 arg1Of μl,25
R12117 T15733 T15734 arg1Of μl,(
R12118 T15733 T15737 arg1Of μl,or
R12119 T15735 T15734 arg2Of BMP-6,(
R12120 T15736 T15734 arg3Of ),(
R12121 T15737 T15731 arg2Of or,of
R12122 T15739 T15737 arg2Of μl,or
R12123 T15739 T15738 arg1Of μl,20
R12124 T15739 T15740 arg1Of μl,(
R12125 T15741 T15740 arg2Of ID1,(
R12126 T15742 T15740 arg3Of ),(
R12127 T15744 T15743 arg1Of cDNA,The
R12128 T15744 T15745 arg2Of cDNA,added
R12129 T15744 T15749 arg1Of cDNA,was
R12130 T15744 T15750 arg1Of cDNA,equivalent
R12131 T15745 T15746 arg1Of added,to
R12132 T15748 T15746 arg2Of reaction,to
R12133 T15748 T15747 arg1Of reaction,each
R12134 T15750 T15749 arg2Of equivalent,was
R12135 T15750 T15751 arg1Of equivalent,to
R12136 T15753 T15751 arg2Of input,to
R12137 T15753 T15752 arg1Of input,the
R12138 T15753 T15754 arg1Of input,of
R12139 T15756 T15754 arg2Of ng,of
R12140 T15756 T15755 arg1Of ng,20
R12141 T15756 T15757 arg1Of ng,of
R12142 T15759 T15757 arg2Of RNA,of
R12143 T15759 T15758 arg1Of RNA,total
R12144 T15762 T15760 arg1Of expression,The
R12145 T15762 T15761 arg1Of expression,gene
R12146 T15762 T15763 arg1Of expression,was
R12147 T15762 T15764 arg2Of expression,quantified
R12148 T15762 T15765 arg1Of expression,using
R12149 T15764 T15763 arg2Of quantified,was
R12150 T15765 T15764 arg3Of using,quantified
R12151 T15765 T15782 arg1Of using,as
R12152 T15769 T15766 arg1Of method,the
R12153 T15769 T15767 arg1Of method,standard
R12154 T15769 T15768 arg1Of method,curve
R12155 T15769 T15770 arg1Of method,(
R12156 T15769 T15774 arg1Of method,or
R12157 T15771 T15770 arg2Of BMP6,(
R12158 T15772 T15770 arg3Of ),(
R12159 T15774 T15765 arg2Of or,using
R12160 T15774 T15773 arg1Of or,","
R12161 T15778 T15774 arg2Of method,or
R12162 T15778 T15775 arg1Of method,the
R12163 T15778 T15776 arg1Of method,comparative
R12164 T15778 T15777 arg1Of method,CT
R12165 T15778 T15779 arg1Of method,(
R12166 T15780 T15779 arg2Of Id1,(
R12167 T15781 T15779 arg3Of ),(
R12168 T15783 T15782 arg2Of described,as
R12169 T15783 T15784 arg1Of described,in
R12170 T15787 T15784 arg2Of Bulletin,in
R12171 T15787 T15785 arg1Of Bulletin,ABI7700
R12172 T15787 T15786 arg1Of Bulletin,User
R12173 T15787 T15788 arg1Of Bulletin,2
R12174 T15787 T15789 arg1Of Bulletin,(
R12175 T15791 T15789 arg2Of Biosystems,(
R12176 T15791 T15790 arg1Of Biosystems,Applied
R12177 T15792 T15789 arg3Of ),(
R12178 T15794 T15793 arg1Of expression,The
R12179 T15794 T15795 arg1Of expression,was
R12180 T15794 T15797 arg2Of expression,normalized
R12181 T15797 T15795 arg2Of normalized,was
R12182 T15797 T15796 arg1Of normalized,then
R12183 T15797 T15798 arg1Of normalized,to
R12184 T15801 T15798 arg2Of level,to
R12185 T15801 T15799 arg1Of level,the
R12186 T15801 T15800 arg1Of level,expression
R12187 T15801 T15802 arg1Of level,of
R12188 T15803 T15802 arg2Of PGK1,of
R12189 T15804 T15805 arg1Of PGK1,was
R12190 T15804 T15806 arg2Of PGK1,chosen
R12191 T15806 T15805 arg2Of chosen,was
R12192 T15806 T15807 arg1Of chosen,","
R12193 T15806 T15808 arg1Of chosen,because
R12194 T15809 T15810 arg1Of it,has
R12195 T15809 T15811 arg1Of it,been
R12196 T15809 T15812 arg2Of it,shown
R12197 T15809 T15814 arg1Of it,have
R12198 T15812 T15808 arg2Of shown,because
R12199 T15812 T15810 arg2Of shown,has
R12200 T15812 T15811 arg2Of shown,been
R12201 T15812 T15821 arg1Of shown,[
R12202 T15814 T15812 arg3Of have,shown
R12203 T15814 T15813 arg1Of have,to
R12204 T15817 T15814 arg2Of variability,have
R12205 T15817 T15815 arg1Of variability,low
R12206 T15817 T15816 arg1Of variability,expression
R12207 T15817 T15818 arg1Of variability,among
R12208 T15820 T15818 arg2Of specimens,among
R12209 T15820 T15819 arg1Of specimens,lymphocyte
R12210 T15822 T15821 arg2Of 56,[
R12211 T15823 T15821 arg3Of ],[
R12212 T15825 T15824 arg1Of levels,Expression
R12213 T15825 T15826 arg1Of levels,in
R12214 T15825 T15829 arg1Of levels,were
R12215 T15825 T15831 arg2Of levels,related
R12216 T15828 T15826 arg2Of cells,in
R12217 T15828 T15827 arg1Of cells,B
R12218 T15831 T15829 arg2Of related,were
R12219 T15831 T15830 arg1Of related,then
R12220 T15831 T15832 arg1Of related,to
R12221 T15835 T15832 arg2Of levels,to
R12222 T15835 T15833 arg1Of levels,the
R12223 T15835 T15834 arg1Of levels,expression
R12224 T15835 T15836 arg1Of levels,in
R12225 T15838 T15836 arg2Of cells,in
R12226 T15838 T15837 arg1Of cells,Ramos
R12526 T16238 T16237 arg1Of proliferation,Cell
R12528 T16240 T16239 arg2Of estimation,For
R12533 T16246 T16254 arg1Of cells,or
R12534 T16248 T16249 arg1Of 7.5,×
R12535 T16250 T16249 arg2Of 104,×
R12537 T16252 T16248 arg1Of ml,7.5
R12538 T16252 T16250 arg1Of ml,104
R12539 T16252 T16251 arg1Of ml,cells/0.2
R12540 T16253 T16247 arg3Of ),(
R12541 T16254 T16264 arg1Of or,were
R12542 T16254 T16265 arg2Of or,cultured
R12543 T16256 T16254 arg2Of cells,or
R12544 T16256 T16255 arg1Of cells,Ramos
R12545 T16256 T16257 arg1Of cells,(
R12546 T16262 T16257 arg2Of ml,(
R12547 T16262 T16258 arg1Of ml,1
R12548 T16262 T16259 arg1Of ml,×
R12549 T16262 T16260 arg1Of ml,104
R12550 T16262 T16261 arg1Of ml,cells/0.2
R12551 T16263 T16257 arg3Of ),(
R12553 T16265 T16239 arg1Of cultured,For
R12554 T16265 T16264 arg2Of cultured,were
R12555 T16265 T16266 arg1Of cultured,in
R12556 T16267 T16266 arg2Of triplicate,in
R12557 T16267 T16268 arg1Of triplicate,in
R12558 T16270 T16268 arg2Of wells,in
R12559 T16270 T16269 arg1Of wells,microtiter
R12560 T16272 T16271 arg1Of cells,The
R12561 T16272 T16273 arg1Of cells,were
R12562 T16272 T16274 arg2Of cells,pulsed
R12563 T16274 T16273 arg2Of pulsed,were
R12564 T16274 T16275 arg1Of pulsed,with
R12565 T16276 T16277 arg1Of 3.7,×
R12566 T16277 T16275 arg2Of ×,with
R12567 T16280 T16279 arg2Of 3H,[
R12568 T16281 T16279 arg3Of ],[
R12569 T16282 T16277 arg2Of thymidine,×
R12570 T16282 T16278 arg1Of thymidine,104Bq
R12571 T16282 T16279 arg1Of thymidine,[
R12572 T16282 T16283 arg1Of thymidine,(
R12573 T16282 T16290 arg1Of thymidine,for
R12574 T16284 T16285 arg1Of Amersham,","
R12575 T16285 T16283 arg2Of ",",(
R12576 T16285 T16287 arg1Of ",",","
R12577 T16286 T16285 arg2Of Buckinghamshire,","
R12578 T16288 T16287 arg2Of UK,","
R12579 T16289 T16283 arg3Of ),(
R12580 T16294 T16290 arg2Of h,for
R12581 T16294 T16291 arg1Of h,the
R12582 T16294 T16292 arg1Of h,last
R12583 T16294 T16293 arg1Of h,16
R12584 T16294 T16295 arg1Of h,of
R12585 T16298 T16295 arg2Of incubation,of
R12586 T16298 T16296 arg1Of incubation,a
R12587 T16298 T16297 arg1Of incubation,72-h
R12588 T16300 T16299 arg1Of cells,The
R12589 T16300 T16301 arg1Of cells,were
R12590 T16300 T16302 arg2Of cells,harvested
R12591 T16302 T16301 arg2Of harvested,were
R12592 T16302 T16319 arg1Of harvested,and
R12593 T16303 T16302 arg3Of using,harvested
R12594 T16303 T16308 arg1Of using,(
R12595 T16307 T16303 arg2Of harvester,using
R12596 T16307 T16304 arg1Of harvester,an
R12597 T16307 T16305 arg2Of harvester,automated
R12598 T16307 T16306 arg1Of harvester,cell
R12599 T16311 T16308 arg2Of Company,(
R12600 T16311 T16309 arg1Of Company,Packard
R12601 T16311 T16310 arg1Of Company,Instrument
R12602 T16311 T16312 arg1Of Company,","
R12603 T16311 T16316 arg1Of Company,","
R12604 T16311 T16317 arg1Of Company,USA
R12605 T16313 T16314 arg1Of Meriden,","
R12606 T16314 T16312 arg2Of ",",","
R12607 T16315 T16314 arg2Of CT,","
R12608 T16318 T16308 arg3Of ),(
R12609 T16321 T16320 arg2Of 3H,[
R12610 T16322 T16320 arg3Of ],[
R12611 T16324 T16320 arg1Of incorporation,[
R12612 T16324 T16323 arg1Of incorporation,thymidine
R12613 T16324 T16325 arg1Of incorporation,was
R12614 T16324 T16326 arg2Of incorporation,determined
R12615 T16326 T16319 arg2Of determined,and
R12616 T16326 T16325 arg2Of determined,was
R12617 T16326 T16327 arg1Of determined,in
R12618 T16326 T16331 arg1Of determined,(
R12619 T16330 T16327 arg2Of counter,in
R12620 T16330 T16328 arg1Of counter,a
R12621 T16330 T16329 arg1Of counter,scintillation
R12622 T16332 T16333 arg1Of TopCount,","
R12623 T16333 T16338 arg1Of ",",","
R12624 T16337 T16333 arg2Of Inc.,","
R12625 T16337 T16334 arg1Of Inc.,Packard
R12626 T16337 T16335 arg1Of Inc.,Instrument
R12627 T16337 T16336 arg1Of Inc.,Company
R12628 T16338 T16331 arg2Of ",",(
R12629 T16338 T16340 arg1Of ",",","
R12630 T16339 T16338 arg2Of Meriden,","
R12631 T16341 T16340 arg2Of CT,","
R12632 T16342 T16331 arg3Of ),(
R12745 T16481 T16482 arg1Of Determination,of
R12746 T16484 T16482 arg2Of death,of
R12747 T16484 T16483 arg1Of death,cell
R12748 T16486 T16485 arg1Of death,Cell
R12749 T16486 T16487 arg1Of death,was
R12750 T16486 T16488 arg2Of death,measured
R12751 T16486 T16495 arg1Of death,staining
R12752 T16488 T16487 arg2Of measured,was
R12753 T16488 T16494 arg1Of measured,by
R12754 T16488 T16518 arg1Of measured,for
R12755 T16493 T16488 arg1Of test,measured
R12756 T16493 T16489 arg2Of test,by
R12757 T16493 T16490 arg1Of test,vital
R12758 T16493 T16491 arg1Of test,dye
R12759 T16493 T16492 arg1Of test,exclusion
R12760 T16495 T16494 arg2Of staining,by
R12761 T16495 T16497 arg1Of staining,with
R12762 T16496 T16495 arg2Of cells,staining
R12763 T16501 T16497 arg2Of iodide,with
R12764 T16501 T16498 arg1Of iodide,5
R12765 T16501 T16499 arg1Of iodide,μg/ml
R12766 T16501 T16500 arg1Of iodide,propidium
R12767 T16501 T16502 arg1Of iodide,(
R12768 T16504 T16503 arg2Of PI,[
R12769 T16504 T16506 arg1Of PI,;
R12770 T16505 T16503 arg3Of ],[
R12771 T16506 T16509 arg1Of ;,;
R12772 T16508 T16506 arg2Of Corp.,;
R12773 T16508 T16507 arg1Of Corp.,Calbiochem
R12774 T16509 T16514 arg1Of ;,;
R12775 T16511 T16509 arg2Of Jolla,;
R12776 T16511 T16510 arg1Of Jolla,La
R12777 T16511 T16512 arg1Of Jolla,","
R12778 T16513 T16512 arg2Of CA,","
R12779 T16514 T16502 arg2Of ;,(
R12780 T16516 T16514 arg2Of mg/ml,;
R12781 T16516 T16515 arg1Of mg/ml,5
R12782 T16517 T16502 arg3Of ),(
R12783 T16520 T16518 arg2Of minute,for
R12784 T16520 T16519 arg1Of minute,one
R12785 T16520 T16521 arg1Of minute,on
R12786 T16522 T16521 arg2Of ice,on
R12787 T16525 T16523 arg1Of "1,000",At
R12788 T16525 T16524 arg1Of "1,000",least
R12789 T16526 T16525 arg1Of cells,"1,000"
R12790 T16526 T16527 arg1Of cells,per
R12791 T16526 T16529 arg1Of cells,were
R12792 T16526 T16530 arg2Of cells,run
R12793 T16528 T16527 arg2Of sample,per
R12794 T16530 T16529 arg2Of run,were
R12795 T16530 T16531 arg1Of run,on
R12796 T16536 T16531 arg2Of cytometer,on
R12797 T16536 T16532 arg1Of cytometer,a
R12798 T16536 T16533 arg1Of cytometer,BD
R12799 T16536 T16534 arg1Of cytometer,FACSCalibur
R12800 T16536 T16535 arg1Of cytometer,flow
R12861 T16610 T16609 arg1Of analysis,Statistical
R12862 T16613 T16611 arg1Of significance,The
R12863 T16613 T16612 arg1Of significance,statistical
R12864 T16613 T16614 arg1Of significance,of
R12865 T16613 T16616 arg1Of significance,between
R12866 T16613 T16618 arg1Of significance,was
R12867 T16613 T16619 arg2Of significance,determined
R12868 T16613 T16629 arg1Of significance,applying
R12869 T16615 T16614 arg2Of differences,of
R12870 T16617 T16616 arg2Of groups,between
R12871 T16619 T16618 arg2Of determined,was
R12872 T16619 T16627 arg1Of determined,","
R12873 T16619 T16628 arg1Of determined,by
R12874 T16620 T16619 arg3Of using,determined
R12875 T16626 T16620 arg2Of test,using
R12876 T16626 T16621 arg1Of test,the
R12877 T16626 T16622 arg1Of test,paired
R12878 T16626 T16623 arg1Of test,two-tailed
R12879 T16626 T16624 arg1Of test,Wilcoxon
R12880 T16626 T16625 arg1Of test,nonparametric
R12881 T16629 T16628 arg2Of applying,by
R12882 T16631 T16629 arg2Of software,applying
R12883 T16631 T16630 arg1Of software,SPSS10.1
R12884 T16631 T16632 arg1Of software,(
R12885 T16634 T16632 arg2Of Inc.,(
R12886 T16634 T16633 arg1Of Inc.,SPSS
R12887 T16634 T16635 arg1Of Inc.,","
R12888 T16634 T16639 arg1Of Inc.,","
R12889 T16634 T16640 arg1Of Inc.,USA
R12890 T16636 T16637 arg1Of Chicago,","
R12891 T16637 T16635 arg2Of ",",","
R12892 T16638 T16637 arg2Of IL,","
R12893 T16641 T16632 arg3Of ),(
R12894 T16643 T16642 arg1Of values,P
R12895 T16643 T16644 arg1Of values,less
R12896 T16643 T16647 arg1Of values,were
R12897 T16643 T16648 arg2Of values,considered
R12898 T16643 T16649 arg1Of values,significant
R12899 T16644 T16645 arg1Of less,than
R12900 T16646 T16645 arg2Of 0.05,than
R12901 T16648 T16647 arg2Of considered,were
R12902 T16649 T16648 arg3Of significant,considered
R12527 T16240 T16241 arg1Of estimation,of
R12529 T16243 T16241 arg2Of synthesis,of
R12530 T16243 T16242 arg1Of synthesis,DNA
R12531 T16246 T16245 arg1Of cells,CD19+
R12532 T16246 T16247 arg1Of cells,(
R12536 T16252 T16247 arg2Of ml,(
R12552 T16265 T16244 arg1Of cultured,","

bionlp-st-ge-2016-coref

Id Subject Object Predicate Lexical cue
T15839 5486-5488 Anaphor denotes it
T15840 5461-5465 Antecedent denotes PGK1
R12227 T15839 T15840 boundBy it,PGK1

bionlp-st-ge-2016-spacy-parsed

Id Subject Object Predicate Lexical cue
T13907 9-13 NNP denotes Cell
T13908 14-21 NN denotes culture
T13909 22-24 IN denotes If
T13910 25-28 RB denotes not
T13911 29-38 VBN denotes specified
T13912 38-39 , denotes ,
T13913 40-43 DT denotes all
T13914 44-49 NNS denotes cells
T13915 50-54 VBD denotes were
T13916 55-63 VBN denotes cultured
T13917 64-66 IN denotes in
T13918 67-73 NNP denotes X-VIVO
T13919 74-76 CD denotes 15
T13920 76-77 NN denotes
T13921 78-79 -LRB- denotes (
T13922 79-91 NNP denotes BioWhittaker
T13923 91-92 , denotes ,
T13924 93-101 NNP denotes Verviers
T13925 101-102 , denotes ,
T13926 103-110 NNP denotes Belgium
T13927 110-111 -RRB- denotes )
T13928 112-122 JJ denotes serum-free
T13929 123-129 NN denotes medium
T13930 130-132 IN denotes at
T13931 133-135 CD denotes 37
T13932 135-136 CD denotes °
T13933 136-137 NNP denotes C
T13934 138-141 CC denotes and
T13935 142-143 CD denotes 5
T13936 143-144 NN denotes %
T13937 145-148 CD denotes CO2
T13938 149-151 IN denotes in
T13939 152-155 NN denotes air
T13940 155-156 . denotes .
T13941 157-167 JJ denotes Peripheral
T13942 168-173 NN denotes blood
T13943 174-177 VBD denotes was
T13944 178-186 VBN denotes provided
T13945 187-189 IN denotes by
T13946 190-193 DT denotes the
T13947 194-199 NNP denotes Blood
T13948 200-204 NNP denotes Bank
T13949 205-207 IN denotes at
T13950 208-216 NNP denotes Buskerud
T13951 217-225 NNP denotes Regional
T13952 226-234 NNP denotes Hospital
T13953 235-239 IN denotes with
T13954 240-246 JJ denotes formal
T13955 247-256 NN denotes agreement
T13956 257-259 IN denotes by
T13957 260-263 DT denotes the
T13958 264-272 NNS denotes patients
T13959 272-273 , denotes ,
T14004 513-514 . denotes .
T14005 515-519 DT denotes This
T14006 520-529 NN denotes procedure
T14007 530-536 NNS denotes yields
T14008 537-541 JJR denotes less
T14009 542-546 IN denotes than
T14010 547-550 CD denotes 0.5
T14011 550-551 NN denotes %
T14012 552-553 NN denotes T
T14013 554-559 NNS denotes cells
T14014 559-560 , denotes ,
T14015 561-564 CD denotes 0.1
T14016 564-565 NN denotes %
T14017 566-568 NNP denotes NK
T14018 569-574 NNS denotes cells
T14019 574-575 , denotes ,
T14020 576-579 CC denotes and
T14021 580-583 CD denotes 0.5
T14022 583-584 NN denotes %
T14023 585-594 NNS denotes monocytes
T14024 595-597 IN denotes as
T14025 598-604 VBN denotes judged
T14026 605-607 IN denotes by
T14027 608-616 JJ denotes indirect
T14028 617-635 NN denotes immunofluorescence
T14029 636-644 NN denotes staining
T14030 644-645 . denotes .
T14031 646-649 DT denotes The
T14032 650-659 VBG denotes following
T14033 660-664 NN denotes cell
T14034 665-670 NNS denotes lines
T14035 671-675 IN denotes from
T14036 676-681 JJ denotes human
T14037 682-690 JJ denotes lymphoid
T14038 691-703 NNS denotes malignancies
T14039 704-708 VBD denotes were
T14040 709-719 VBN denotes maintained
T14041 720-722 IN denotes in
T14042 723-727 NNP denotes RPMI
T14043 728-732 CD denotes 1640
T14044 733-734 -LRB- denotes (
T14045 734-737 NNP denotes PAA
T14046 738-750 NNPS denotes Laboratories
T14047 751-755 NNP denotes GmbH
T14048 755-756 , denotes ,
T14049 757-765 NNP denotes Pasching
T14050 765-766 , denotes ,
T14051 767-774 NNP denotes Austria
T14052 774-775 -RRB- denotes )
T14053 776-788 VBN denotes supplemented
T14054 789-793 IN denotes with
T14055 794-796 CD denotes 10
T14056 796-797 NN denotes %
T14057 798-804 JJ denotes foetal
T14058 805-811 JJ denotes bovine
T14059 812-817 NN denotes serum
T14060 818-819 -LRB- denotes (
T14061 819-822 NNP denotes FCS
T14062 822-823 -RRB- denotes )
T14063 823-824 , denotes ,
T14064 825-828 CD denotes 100
T14065 829-837 NN denotes units/ml
T14066 838-848 NN denotes penicillin
T14067 849-850 NNP denotes G
T14068 850-851 , denotes ,
T14069 852-855 CC denotes and
T14070 856-859 CD denotes 100
T14071 860-868 NN denotes units/ml
T14072 869-871 IN denotes of
T14073 872-884 NNP denotes streptomycin
T14074 885-893 NN denotes sulphate
T14075 893-894 , denotes ,
T14076 895-898 CC denotes but
T14077 899-912 JJ denotes serum-starved
T14078 913-916 IN denotes for
T14079 917-919 IN denotes at
T14080 920-925 JJS denotes least
T14081 926-930 CD denotes four
T14082 931-936 NNS denotes hours
T14083 937-940 CC denotes and
T14084 941-949 VBN denotes cultured
T14085 950-952 IN denotes in
T14086 953-959 NNP denotes X-VIVO
T14087 960-962 CD denotes 15
T14088 962-963 NN denotes
T14089 964-968 WRB denotes when
T14090 969-977 VBN denotes included
T14091 978-980 IN denotes in
T14092 981-992 NNS denotes experiments
T14093 992-993 : denotes :
T14094 994-1006 JJ denotes EBV-negative
T14095 1007-1009 NNP denotes BL
T14096 1010-1014 NN denotes cell
T14097 1015-1020 NNS denotes lines
T14098 1021-1026 NNP denotes Ramos
T14099 1027-1028 -LRB- denotes (
T14100 1028-1033 NNP denotes ECACC
T14101 1034-1042 CD denotes 85030802
T14102 1042-1043 -RRB- denotes )
T14103 1043-1044 , denotes ,
T14104 1045-1049 NNP denotes HL60
T14105 1050-1051 -LRB- denotes (
T14106 1051-1059 NNP denotes JCRB0085
T14107 1059-1060 -RRB- denotes )
T14108 1060-1061 . denotes .
T14167 1063-1069 NN denotes Growth
T14168 1070-1089 NNS denotes factors/supplements
T14169 1090-1093 DT denotes The
T14170 1094-1103 JJ denotes following
T14171 1104-1112 NNS denotes reagents
T14172 1113-1117 VBD denotes were
T14173 1118-1122 VBN denotes used
T14174 1123-1125 IN denotes at
T14175 1126-1135 JJ denotes indicated
T14176 1136-1150 NNS denotes concentrations
T14177 1150-1151 : denotes :
T14178 1152-1163 JJ denotes recombinant
T14179 1164-1169 JJ denotes human
T14180 1170-1171 -LRB- denotes (
T14181 1171-1174 NN denotes rhu
T14182 1174-1175 -RRB- denotes )
T14183 1176-1181 NN denotes BMP-6
T14184 1182-1183 -LRB- denotes (
T14185 1183-1184 CD denotes 1
T14186 1185-1187 NN denotes μg
T14187 1187-1188 NN denotes /
T14188 1188-1190 NN denotes ml
T14189 1190-1191 , denotes ,
T14190 1192-1194 IN denotes if
T14191 1195-1198 RB denotes not
T14192 1199-1208 VBN denotes specified
T14193 1209-1218 RB denotes otherwise
T14194 1218-1219 -RRB- denotes )
T14195 1219-1220 , denotes ,
T14196 1221-1224 FW denotes rhu
T14197 1225-1236 NNP denotes BMP-RIB/ALK
T14198 1236-1238 CD denotes -6
T14199 1238-1239 CD denotes /
T14200 1239-1241 NNP denotes Fc
T14201 1242-1249 NNP denotes Chimera
T14202 1250-1251 -LRB- denotes (
T14203 1251-1252 CD denotes 5
T14204 1253-1255 NN denotes μg
T14205 1255-1256 NN denotes /
T14206 1256-1258 NN denotes ml
T14207 1258-1259 -RRB- denotes )
T14208 1259-1260 , denotes ,
T14209 1261-1264 FW denotes rhu
T14210 1265-1275 NNP denotes BMPR-II/Fc
T14211 1276-1283 NNP denotes Chimera
T14212 1284-1285 -LRB- denotes (
T14213 1285-1286 CD denotes 5
T14214 1287-1289 NN denotes μg
T14215 1289-1290 NN denotes /
T14216 1290-1292 NN denotes ml
T14217 1292-1293 -RRB- denotes )
T14218 1293-1294 , denotes ,
T14219 1295-1298 CC denotes and
T14220 1299-1310 JJ denotes recombinant
T14221 1311-1316 NN denotes mouse
T14222 1317-1323 NNP denotes Noggin
T14223 1324-1325 -LRB- denotes (
T14224 1325-1326 CD denotes 5
T14225 1327-1329 NN denotes μg
T14226 1329-1330 NN denotes /
T14227 1330-1332 NN denotes ml
T14228 1332-1333 -RRB- denotes )
T14229 1334-1338 VBD denotes were
T14230 1339-1348 VBN denotes purchased
T14231 1349-1353 IN denotes from
T14232 1354-1357 NNP denotes R&D
T14233 1358-1365 NNP denotes Systems
T14234 1366-1367 -LRB- denotes (
T14235 1367-1375 NNP denotes Abingdon
T14236 1375-1376 , denotes ,
T14237 1377-1379 NNP denotes UK
T14238 1379-1380 -RRB- denotes )
T14239 1380-1381 : denotes ;
T14240 1382-1390 NNP denotes Anti-IgM
T14241 1391-1392 NN denotes F
T14242 1392-1393 -LRB- denotes (
T14243 1393-1395 NN denotes ab
T14244 1395-1396 -RRB- denotes )
T14245 1396-1397 CD denotes 2
T14246 1398-1407 NNS denotes fragments
T14247 1408-1410 IN denotes of
T14248 1411-1417 NN denotes rabbit
T14249 1418-1428 NN denotes polyclonal
T14250 1429-1439 NNS denotes antibodies
T14251 1440-1442 TO denotes to
T14252 1443-1448 JJ denotes human
T14253 1449-1452 NNP denotes IgM
T14254 1453-1458 NN denotes heavy
T14255 1459-1464 NN denotes chain
T14256 1465-1466 -LRB- denotes (
T14257 1466-1470 CD denotes 37.5
T14258 1471-1473 NN denotes μg
T14259 1473-1474 NN denotes /
T14260 1474-1476 NN denotes ml
T14261 1476-1477 -RRB- denotes )
T14262 1478-1481 VBD denotes was
T14263 1482-1490 VBN denotes obtained
T14264 1491-1495 IN denotes from
T14265 1496-1500 NNP denotes Dako
T14266 1500-1501 , denotes ,
T14267 1502-1512 NNP denotes Copenhagen
T14268 1512-1513 , denotes ,
T14269 1514-1521 NNP denotes Denmark
T14270 1522-1525 CC denotes and
T14271 1526-1529 NNP denotes rhu
T14272 1530-1534 NNP denotes CD40
T14273 1535-1541 NN denotes ligand
T14274 1542-1543 -LRB- denotes (
T14275 1543-1548 NNP denotes CD40L
T14276 1548-1549 , denotes ,
T14277 1550-1552 CD denotes 10
T14278 1553-1558 NN denotes ng/ml
T14279 1558-1559 -RRB- denotes )
T14280 1560-1563 VBD denotes was
T14281 1564-1565 DT denotes a
T14282 1566-1570 NN denotes gift
T14283 1571-1575 IN denotes from
T14284 1576-1583 NNP denotes Immunex
T14285 1584-1589 NNP denotes Corp.
T14286 1590-1591 -LRB- denotes (
T14287 1591-1598 NNP denotes Seattle
T14288 1598-1599 , denotes ,
T14289 1600-1602 NNP denotes WA
T14290 1602-1603 -RRB- denotes )
T14291 1603-1604 . denotes .
T14718 1606-1616 NNS denotes Antibodies
T14719 1617-1621 VBD denotes used
T14720 1622-1625 IN denotes for
T14721 1626-1630 NN denotes flow
T14722 1631-1641 JJ denotes cytometric
T14723 1642-1650 NN denotes analysis
T14724 1651-1654 CC denotes and
T14725 1655-1665 NN denotes immunoblot
T14726 1666-1674 NN denotes analysis
T14727 1675-1685 NNS denotes Antibodies
T14728 1686-1693 IN denotes against
T14729 1694-1697 DT denotes the
T14730 1698-1703 JJ denotes human
T14731 1704-1717 NNS denotes BMP-receptors
T14732 1718-1725 NNP denotes Act-RIA
T14733 1725-1726 , denotes ,
T14734 1727-1734 NNP denotes BMP-RIB
T14735 1734-1735 , denotes ,
T14736 1736-1743 NNP denotes BMPR-II
T14737 1743-1744 , denotes ,
T14738 1745-1753 NNP denotes Act-RIIA
T14739 1754-1757 CC denotes and
T14740 1758-1766 NNP denotes Act-RIIb
T14741 1767-1771 VBD denotes were
T14742 1772-1781 VBN denotes purchased
T14743 1782-1786 IN denotes from
T14744 1787-1788 NN denotes R
T14745 1789-1790 CC denotes &
T14746 1791-1792 NNP denotes D
T14747 1793-1800 NNP denotes Systems
T14748 1801-1802 -LRB- denotes (
T14749 1802-1810 NNP denotes Abingdon
T14750 1810-1811 , denotes ,
T14751 1812-1814 NNP denotes UK
T14752 1814-1815 -RRB- denotes )
T14753 1815-1816 . denotes .
T14754 1817-1826 NNP denotes Detection
T14755 1827-1829 IN denotes of
T14756 1830-1833 DT denotes the
T14757 1834-1847 NN denotes BMP-6-protein
T14758 1848-1851 VBZ denotes has
T14759 1852-1856 VBN denotes been
T14760 1857-1862 VBN denotes tried
T14761 1863-1867 IN denotes with
T14762 1868-1871 DT denotes the
T14763 1872-1881 VBG denotes following
T14764 1882-1892 NNS denotes antibodies
T14765 1892-1893 : denotes :
T14766 1894-1898 VB denotes goat
T14767 1899-1909 JJ denotes polyclonal
T14768 1910-1920 NN denotes anti-BMP-6
T14769 1921-1922 -LRB- denotes (
T14770 1922-1927 NNP denotes Santa
T14771 1928-1932 NNP denotes Cruz
T14772 1932-1933 , denotes ,
T14773 1934-1937 NNP denotes San
T14774 1938-1943 NNP denotes Diego
T14775 1943-1944 , denotes ,
T14776 1945-1947 NNP denotes CA
T14777 1947-1948 , denotes ,
T14778 1949-1952 NNP denotes USA
T14779 1952-1953 -RRB- denotes )
T14780 1953-1954 , denotes ,
T14781 1955-1965 JJ denotes monoclonal
T14782 1966-1971 NN denotes mouse
T14783 1972-1982 NN denotes anti-BMP-6
T14784 1983-1986 CC denotes and
T14785 1987-1997 JJ denotes polyclonal
T14786 1998-2002 NN denotes goat
T14787 2003-2013 NN denotes anti-BMP-6
T14788 2014-2018 IN denotes from
T14789 2019-2020 NNP denotes R
T14790 2021-2022 CC denotes &
T14791 2023-2024 NNP denotes D
T14792 2025-2032 NNP denotes Systems
T14793 2033-2034 -LRB- denotes (
T14794 2034-2042 NNP denotes Abingdon
T14795 2042-2043 , denotes ,
T14796 2044-2046 NNP denotes UK
T14797 2046-2047 -RRB- denotes )
T14798 2047-2048 , denotes ,
T14799 2049-2052 CC denotes and
T14800 2053-2058 NN denotes mouse
T14801 2059-2069 JJ denotes monoclonal
T14802 2070-2080 NN denotes anti-BMP-6
T14803 2081-2082 -LRB- denotes (
T14804 2082-2090 NNP denotes Chemicon
T14805 2091-2104 NNP denotes International
T14806 2105-2108 NNP denotes Inc
T14807 2108-2109 , denotes ,
T14808 2110-2118 NNP denotes Temecula
T14809 2118-2119 , denotes ,
T14810 2120-2122 NNP denotes CA
T14811 2122-2123 , denotes ,
T14812 2124-2127 NNP denotes USA
T14813 2127-2128 -RRB- denotes )
T14814 2128-2129 . denotes .
T14815 2130-2146 NNP denotes Characterisation
T14816 2147-2149 IN denotes of
T14817 2150-2164 JJ denotes BMP-signalling
T14818 2165-2173 NNS denotes pathways
T14819 2174-2177 VBD denotes was
T14820 2178-2182 VBN denotes done
T14821 2183-2185 IN denotes by
T14822 2186-2189 NN denotes use
T14823 2190-2192 IN denotes of
T14824 2193-2211 JJ denotes anti-phospho-Smad1
T14825 2211-2212 , denotes ,
T14826 2213-2215 CD denotes -5
T14827 2215-2216 , denotes ,
T14828 2217-2218 : denotes -
T14829 2218-2221 CC denotes and
T14830 2222-2223 CD denotes 8
T14831 2224-2234 JJ denotes polyclonal
T14832 2235-2243 NN denotes antibody
T14833 2244-2245 -LRB- denotes (
T14834 2245-2253 NNP denotes Chemicon
T14835 2253-2254 , denotes ,
T14836 2255-2263 NNP denotes Temecula
T14837 2263-2264 , denotes ,
T14838 2265-2267 NNP denotes CA
T14839 2267-2268 , denotes ,
T14840 2269-2272 NNP denotes USA
T14841 2272-2273 -RRB- denotes )
T14842 2273-2274 . denotes .
T14843 2275-2285 NN denotes Expression
T14844 2286-2292 NNS denotes levels
T14845 2293-2295 IN denotes of
T14846 2296-2299 NNP denotes Id1
T14847 2300-2301 CD denotes 3
T14848 2302-2310 NNS denotes proteins
T14849 2311-2315 VBD denotes were
T14850 2316-2324 VBN denotes detected
T14851 2325-2329 IN denotes with
T14852 2330-2340 JJ denotes polyclonal
T14853 2341-2347 NN denotes rabbit
T14854 2348-2356 NN denotes antibody
T14855 2357-2360 CC denotes and
T14856 2361-2370 NN denotes detection
T14857 2371-2374 VBD denotes was
T14858 2375-2382 VBN denotes blocked
T14859 2383-2387 IN denotes with
T14860 2388-2396 VBG denotes blocking
T14896 2591-2593 NNP denotes AS
T14897 2594-2595 -LRB- denotes (
T14898 2595-2605 NNP denotes Copenhagen
T14899 2605-2606 , denotes ,
T14900 2607-2614 NNP denotes Denmark
T14901 2614-2615 -RRB- denotes )
T14902 2616-2619 IN denotes for
T14903 2620-2630 NN denotes immunoblot
T14904 2631-2639 NN denotes analysis
T14905 2639-2640 . denotes .
T14906 2641-2653 NN denotes Anti-β-actin
T14907 2654-2657 VBD denotes was
T14908 2658-2662 IN denotes from
T14909 2663-2673 NNP denotes Santa-Cruz
T14910 2673-2674 . denotes .
T14911 2675-2679 IN denotes From
T14912 2680-2686 NNP denotes Becton
T14913 2687-2696 NNP denotes Dickinson
T14914 2697-2698 -LRB- denotes (
T14915 2698-2701 NNP denotes San
T14916 2702-2706 NNP denotes Jose
T14917 2706-2707 , denotes ,
T14918 2708-2710 NNP denotes CA
T14919 2710-2711 -RRB- denotes )
T14920 2711-2712 , denotes ,
T14921 2713-2715 PRP denotes we
T14922 2716-2725 VBD denotes purchased
T14923 2726-2738 JJ denotes anti-CD19-PE
T14959 2930-2933 NNP denotes USA
T14960 2933-2934 -RRB- denotes )
T14961 2934-2935 . denotes .
T15151 2937-2941 NNP denotes Cell
T15152 2942-2949 VBG denotes sorting
T15153 2950-2956 NNP denotes Highly
T15154 2957-2965 VBD denotes purified
T15155 2966-2970 NNP denotes CD19
T15156 2970-2971 NNP denotes +
T15157 2971-2975 NNP denotes CD27
T15158 2975-2976 : denotes -
T15159 2977-2979 CC denotes or
T15160 2980-2984 CD denotes CD19
T15161 2984-2985 CD denotes +
T15162 2985-2989 NNP denotes CD27
T15163 2989-2990 NN denotes +
T15164 2991-2996 NNS denotes cells
T15165 2997-3001 VBD denotes were
T15166 3002-3010 VBN denotes obtained
T15167 3011-3013 IN denotes by
T15168 3014-3022 VBG denotes staining
T15169 3023-3027 NNP denotes CD19
T15170 3027-3028 CD denotes +
T15171 3029-3034 NNS denotes cells
T15172 3035-3039 IN denotes with
T15173 3040-3049 JJ denotes anti-CD27
T15174 3050-3052 NNP denotes PE
T15175 3053-3056 CC denotes and
T15176 3057-3061 NNP denotes CD19
T15177 3062-3065 NNP denotes PC5
T15178 3066-3070 NNP denotes mAbs
T15179 3071-3074 IN denotes for
T15180 3075-3077 CD denotes 30
T15181 3078-3085 NNS denotes minutes
T15182 3086-3088 IN denotes at
T15183 3089-3090 CD denotes 4
T15184 3090-3091 CD denotes °
T15185 3091-3092 NNP denotes C
T15186 3092-3093 , denotes ,
T15187 3094-3102 VBN denotes followed
T15188 3103-3105 IN denotes by
T15189 3106-3113 VBG denotes washing
T15190 3114-3118 IN denotes with
T15191 3119-3122 NNP denotes PBS
T15192 3123-3126 CC denotes and
T15193 3127-3134 VBG denotes sorting
T15194 3135-3137 IN denotes on
T15195 3138-3142 NNP denotes FACS
T15196 3143-3147 NNP denotes DiVa
T15197 3148-3152 IN denotes from
T15198 3153-3159 NNP denotes Becton
T15199 3160-3169 NNP denotes Dickinson
T15200 3169-3170 . denotes .
T15878 4314-4322 NN denotes Analysis
T15879 4323-4325 IN denotes of
T15880 4326-4331 NNP denotes BMP-6
T15881 4332-4341 NN denotes messenger
T15882 4342-4345 NNP denotes RNA
T15883 4346-4347 -LRB- denotes (
T15884 4347-4351 NNP denotes mRNA
T15885 4351-4352 -RRB- denotes )
T15886 4353-4363 NN denotes expression
T15887 4364-4374 JJ denotes Endogenous
T15888 4375-4385 NN denotes expression
T15889 4386-4388 IN denotes of
T15890 4389-4392 DT denotes the
T15891 4393-4398 NNP denotes BMP-6
T15892 4399-4403 NN denotes gene
T15893 4404-4407 VBD denotes was
T15894 4408-4416 VBN denotes examined
T15895 4417-4419 IN denotes by
T15896 4420-4427 JJ denotes reverse
T15897 4428-4452 NN denotes transcription-polymerase
T15898 4453-4458 NN denotes chain
T15899 4459-4467 NN denotes reaction
T15900 4467-4468 . denotes .
T15901 4469-4474 JJ denotes Total
T15902 4475-4478 NNP denotes RNA
T15903 4479-4482 VBD denotes was
T15904 4483-4491 VBN denotes isolated
T15905 4492-4497 VBG denotes using
T15906 4498-4508 RB denotes Absolutely
T15907 4509-4512 NNP denotes RNA
T15908 4512-4513 CD denotes
T15909 4514-4520 NNP denotes RT-PCR
T15910 4521-4529 NNP denotes Miniprep
T15911 4530-4533 NNP denotes Kit
T15912 4534-4535 -LRB- denotes (
T15913 4535-4545 NNP denotes Stratagene
T15914 4546-4552 NNP denotes Europe
T15915 4552-4553 , denotes ,
T15916 4554-4563 NNP denotes Amsterdam
T15917 4563-4564 , denotes ,
T15918 4565-4575 NNP denotes Netherland
T15919 4575-4576 -RRB- denotes )
T15920 4577-4586 VBG denotes according
T15921 4587-4589 TO denotes to
T15922 4590-4593 DT denotes the
T15923 4594-4607 NNS denotes manufacturers
T15924 4608-4620 NNS denotes instructions
T15925 4620-4621 . denotes .
T15926 4622-4636 NN denotes Quantification
T15927 4637-4639 IN denotes of
T15928 4640-4643 DT denotes the
T15929 4644-4652 JJ denotes isolated
T15930 4653-4658 JJ denotes total
T15931 4659-4662 NNP denotes RNA
T15932 4663-4666 VBD denotes was
T15933 4667-4675 VBN denotes achieved
T15934 4676-4678 IN denotes by
T15935 4679-4684 VBG denotes using
T15936 4685-4703 JJ denotes spectrophotometric
T15937 4704-4709 NNP denotes OD260
T15938 4710-4722 NNS denotes measurements
T15939 4722-4723 . denotes .
T15940 4724-4729 NNP denotes Equal
T15941 4730-4737 NNS denotes amounts
T15942 4738-4740 IN denotes of
T15943 4741-4744 NNP denotes RNA
T15944 4745-4749 VBD denotes were
T15945 4750-4754 RB denotes then
T15946 4755-4762 VB denotes reverse
T15947 4763-4774 VBN denotes transcribed
T15948 4775-4777 TO denotes to
T15949 4778-4782 VB denotes cDNA
T15950 4783-4787 IN denotes with
T15951 4788-4794 NNP denotes TaqMan
T15952 4794-4795 NNP denotes ®
T15953 4796-4803 VB denotes Reverse
T15954 4804-4817 NNP denotes Transcription
T15955 4818-4826 NNP denotes Reagents
T15956 4827-4828 -LRB- denotes (
T15957 4828-4835 NNP denotes Applied
T15958 4836-4846 NNPS denotes Biosystems
T15959 4846-4847 , denotes ,
T15960 4848-4854 NNP denotes Foster
T15961 4855-4859 NNP denotes City
T15962 4859-4860 , denotes ,
T15963 4861-4863 NNP denotes CA
T15964 4863-4864 , denotes ,
T15965 4865-4870 NNP denotes U.S.A
T15966 4870-4871 -RRB- denotes )
T15967 4871-4872 . denotes .
T15968 4873-4875 TO denotes To
T15969 4876-4883 VB denotes measure
T15970 4884-4888 NNP denotes mRNA
T15971 4889-4899 NN denotes expression
T15972 4900-4902 IN denotes of
T15973 4903-4907 NNP denotes BMP6
T15974 4907-4908 , denotes ,
T15975 4909-4912 NNP denotes Id1
T15976 4913-4916 NNP denotes Id4
T15977 4917-4920 CC denotes and
T15978 4921-4925 NNP denotes PGK1
T15979 4926-4929 NNP denotes PCR
T15980 4930-4934 VBD denotes were
T15981 4935-4942 VBN denotes carried
T15982 4943-4946 RP denotes out
T15983 4947-4951 IN denotes with
T15984 4952-4958 NNP denotes TaqMan
T15985 4958-4959 VBD denotes ®
T15986 4960-4969 JJ denotes universal
T15987 4970-4976 NN denotes master
T15988 4977-4980 NN denotes mix
T15989 4980-4981 . denotes .
T15990 4982-4989 NNS denotes Primers
T15991 4990-4993 CC denotes and
T15992 4994-5000 NNS denotes probes
T15993 5001-5005 VBD denotes were
T15994 5006-5014 VBN denotes provided
T15995 5015-5017 IN denotes by
T15996 5018-5033 NNP denotes Assay-on-Demand
T15997 5034-5035 -LRB- denotes (
T15998 5035-5042 NNP denotes Applied
T15999 5043-5053 NNPS denotes Biosystems
T16000 5053-5054 -RRB- denotes )
T16001 5054-5055 . denotes .
T16002 5056-5059 NNP denotes PCR
T16003 5060-5069 NNS denotes reactions
T16004 5070-5074 VBD denotes were
T16005 5075-5082 VBN denotes carried
T16006 5083-5086 RP denotes out
T16007 5087-5089 IN denotes in
T16008 5090-5091 DT denotes a
T16009 5092-5097 JJ denotes final
T16010 5098-5104 NN denotes volume
T16011 5105-5107 IN denotes of
T16012 5108-5110 CD denotes 25
T16013 5111-5113 NN denotes μl
T16014 5114-5115 -LRB- denotes (
T16015 5115-5120 NNP denotes BMP-6
T16016 5120-5121 -RRB- denotes )
T16017 5122-5124 CC denotes or
T16018 5125-5127 CD denotes 20
T16019 5128-5130 NN denotes μl
T16020 5131-5132 -LRB- denotes (
T16021 5132-5135 CD denotes ID1
T16022 5135-5136 -RRB- denotes )
T16023 5136-5137 . denotes .
T16024 5138-5141 DT denotes The
T16025 5142-5146 NNP denotes cDNA
T16026 5147-5152 VBD denotes added
T16027 5153-5155 TO denotes to
T16028 5156-5160 DT denotes each
T16029 5161-5169 NN denotes reaction
T16030 5170-5173 VBD denotes was
T16031 5174-5184 JJ denotes equivalent
T16032 5185-5187 TO denotes to
T16033 5188-5191 DT denotes the
T16034 5192-5197 NN denotes input
T16035 5198-5200 IN denotes of
T16036 5201-5203 CD denotes 20
T16037 5204-5206 NN denotes ng
T16038 5207-5209 IN denotes of
T16039 5210-5215 JJ denotes total
T16040 5216-5219 NNP denotes RNA
T16041 5219-5220 . denotes .
T16042 5221-5224 DT denotes The
T16043 5225-5229 NN denotes gene
T16044 5230-5240 NN denotes expression
T16045 5241-5244 VBD denotes was
T16046 5245-5255 VBN denotes quantified
T16047 5256-5261 VBG denotes using
T16048 5262-5265 DT denotes the
T16049 5266-5274 JJ denotes standard
T16050 5275-5280 NN denotes curve
T16051 5281-5287 NN denotes method
T16052 5288-5289 -LRB- denotes (
T16053 5289-5293 NNP denotes BMP6
T16054 5293-5294 -RRB- denotes )
T16055 5294-5295 , denotes ,
T16056 5296-5298 CC denotes or
T16057 5299-5302 DT denotes the
T16058 5303-5314 JJ denotes comparative
T16059 5315-5317 NNP denotes CT
T16060 5318-5324 NN denotes method
T16061 5325-5326 -LRB- denotes (
T16062 5326-5329 NNP denotes Id1
T16063 5329-5330 -RRB- denotes )
T16064 5331-5333 IN denotes as
T16065 5334-5343 VBN denotes described
T16066 5344-5346 IN denotes in
T16067 5347-5354 NNP denotes ABI7700
T16068 5355-5359 NNP denotes User
T16069 5360-5368 NNP denotes Bulletin
T16070 5369-5370 CD denotes 2
T16071 5371-5372 -LRB- denotes (
T16072 5372-5379 NNP denotes Applied
T16073 5380-5390 NNPS denotes Biosystems
T16074 5390-5391 -RRB- denotes )
T16075 5391-5392 . denotes .
T16076 5393-5396 DT denotes The
T16077 5397-5407 NN denotes expression
T16078 5408-5411 VBD denotes was
T16079 5412-5416 RB denotes then
T16080 5417-5427 VBN denotes normalized
T16081 5428-5430 TO denotes to
T16082 5431-5434 DT denotes the
T16083 5435-5445 NN denotes expression
T16084 5446-5451 NN denotes level
T16085 5452-5454 IN denotes of
T16086 5455-5459 NNP denotes PGK1
T16087 5459-5460 . denotes .
T16088 5461-5465 CD denotes PGK1
T16089 5466-5469 VBD denotes was
T16090 5470-5476 VBN denotes chosen
T16091 5476-5477 , denotes ,
T16092 5478-5485 IN denotes because
T16093 5486-5488 PRP denotes it
T16094 5489-5492 VBZ denotes has
T16095 5493-5497 VBN denotes been
T16096 5498-5503 VBN denotes shown
T16097 5504-5506 TO denotes to
T16098 5507-5511 VB denotes have
T16099 5512-5515 JJ denotes low
T16100 5516-5526 NN denotes expression
T16101 5527-5538 NN denotes variability
T16102 5539-5544 IN denotes among
T16103 5545-5555 JJ denotes lymphocyte
T16104 5556-5565 NNS denotes specimens
T16105 5566-5567 NNP denotes [
T16106 5567-5569 CD denotes 56
T16107 5569-5570 NNP denotes ]
T16108 5570-5571 . denotes .
T16109 5572-5582 NN denotes Expression
T16110 5583-5589 NNS denotes levels
T16111 5590-5592 IN denotes in
T16112 5593-5594 NNP denotes B
T16113 5595-5600 NNS denotes cells
T16114 5601-5605 VBD denotes were
T16115 5606-5610 RB denotes then
T16116 5611-5618 VBN denotes related
T16117 5619-5621 TO denotes to
T16118 5622-5625 DT denotes the
T16119 5626-5636 NN denotes expression
T16120 5637-5643 NNS denotes levels
T16121 5644-5646 IN denotes in
T16122 5647-5652 NNP denotes Ramos
T16123 5653-5658 NNS denotes cells
T16124 5658-5659 . denotes .
T16348 5661-5665 NNP denotes Cell
T16349 5666-5679 NN denotes proliferation
T16350 5680-5683 IN denotes For
T16351 5684-5694 NN denotes estimation
T16352 5695-5697 IN denotes of
T16353 5698-5701 NNP denotes DNA
T16354 5702-5711 NN denotes synthesis
T16355 5711-5712 , denotes ,
T16356 5713-5717 NNP denotes CD19
T16357 5717-5718 NNP denotes +
T16358 5719-5724 NNS denotes cells
T16359 5725-5726 -LRB- denotes (
T16360 5726-5729 CD denotes 7.5
T16361 5730-5731 NN denotes ×
T16362 5732-5735 CD denotes 104
T16363 5736-5743 CD denotes cells/0
T16364 5743-5745 CD denotes .2
T16365 5746-5748 NN denotes ml
T16366 5748-5749 -RRB- denotes )
T16367 5750-5752 CC denotes or
T16368 5753-5758 NNP denotes Ramos
T16369 5759-5764 NNS denotes cells
T16370 5765-5766 -LRB- denotes (
T16371 5766-5767 CD denotes 1
T16372 5768-5769 CD denotes ×
T16373 5770-5773 CD denotes 104
T16374 5774-5781 CD denotes cells/0
T16375 5781-5783 CD denotes .2
T16376 5784-5786 NN denotes ml
T16377 5786-5787 -RRB- denotes )
T16378 5788-5792 VBD denotes were
T16379 5793-5801 VBN denotes cultured
T16380 5802-5804 IN denotes in
T16381 5805-5815 NN denotes triplicate
T16382 5816-5818 IN denotes in
T16383 5819-5829 NN denotes microtiter
T16384 5830-5835 NNS denotes wells
T16385 5835-5836 . denotes .
T16386 5837-5840 DT denotes The
T16387 5841-5846 NNS denotes cells
T16388 5847-5851 VBD denotes were
T16389 5852-5858 VBN denotes pulsed
T16390 5859-5863 IN denotes with
T16391 5864-5867 CD denotes 3.7
T16392 5868-5869 CD denotes ×
T16393 5870-5875 NNP denotes 104Bq
T16394 5876-5877 NNP denotes [
T16395 5877-5879 NNP denotes 3H
T16396 5879-5880 NNP denotes ]
T16397 5880-5889 NN denotes thymidine
T16398 5890-5891 -LRB- denotes (
T16399 5891-5899 NNP denotes Amersham
T16400 5899-5900 , denotes ,
T16401 5901-5916 NNP denotes Buckinghamshire
T16402 5916-5917 , denotes ,
T16403 5918-5920 NNP denotes UK
T16404 5920-5921 -RRB- denotes )
T16405 5922-5925 IN denotes for
T16406 5926-5929 DT denotes the
T16407 5930-5934 JJ denotes last
T16408 5935-5937 CD denotes 16
T16409 5938-5939 NN denotes h
T16410 5940-5942 IN denotes of
T16411 5943-5944 DT denotes a
T16412 5945-5949 JJ denotes 72-h
T16413 5950-5960 NN denotes incubation
T16414 5960-5961 . denotes .
T16415 5962-5965 DT denotes The
T16416 5966-5971 NNS denotes cells
T16417 5972-5976 VBD denotes were
T16418 5977-5986 VBN denotes harvested
T16419 5987-5992 VBG denotes using
T16420 5993-5995 DT denotes an
T16421 5996-6005 VBN denotes automated
T16422 6006-6010 NN denotes cell
T16423 6011-6020 NN denotes harvester
T16424 6021-6022 -LRB- denotes (
T16425 6022-6029 NNP denotes Packard
T16426 6030-6040 NNP denotes Instrument
T16427 6041-6048 NNP denotes Company
T16428 6048-6049 , denotes ,
T16429 6050-6057 NNP denotes Meriden
T16430 6057-6058 , denotes ,
T16431 6059-6061 NNP denotes CT
T16432 6061-6062 , denotes ,
T16433 6063-6066 NNP denotes USA
T16434 6066-6067 -RRB- denotes )
T16435 6068-6071 CC denotes and
T16436 6072-6073 NNP denotes [
T16437 6073-6075 NNP denotes 3H
T16438 6075-6076 NNP denotes ]
T16439 6076-6085 NN denotes thymidine
T16440 6086-6099 NN denotes incorporation
T16441 6100-6103 VBD denotes was
T16442 6104-6114 VBN denotes determined
T16443 6115-6117 IN denotes in
T16444 6118-6119 DT denotes a
T16445 6120-6133 NN denotes scintillation
T16446 6134-6141 NN denotes counter
T16447 6142-6143 -LRB- denotes (
T16448 6143-6151 NNP denotes TopCount
T16449 6151-6152 , denotes ,
T16450 6153-6160 NNP denotes Packard
T16451 6161-6171 NNP denotes Instrument
T16452 6172-6179 NNP denotes Company
T16453 6180-6184 NNP denotes Inc.
T16454 6184-6185 , denotes ,
T16455 6186-6193 NNP denotes Meriden
T16456 6193-6194 , denotes ,
T16457 6195-6197 NNP denotes CT
T16458 6197-6198 -RRB- denotes )
T16459 6198-6199 . denotes .
T16540 6201-6214 NN denotes Determination
T16541 6215-6217 IN denotes of
T16542 6218-6222 NN denotes cell
T16543 6223-6228 NN denotes death
T16544 6229-6233 NNP denotes Cell
T16545 6234-6239 NN denotes death
T16546 6240-6243 VBD denotes was
T16547 6244-6252 VBN denotes measured
T16548 6253-6255 IN denotes by
T16549 6256-6261 JJ denotes vital
T16550 6262-6265 NN denotes dye
T16551 6266-6275 NN denotes exclusion
T16552 6276-6280 NN denotes test
T16553 6281-6283 IN denotes by
T16554 6284-6292 VBG denotes staining
T16555 6293-6298 NNS denotes cells
T16556 6299-6303 IN denotes with
T16557 6304-6305 CD denotes 5
T16558 6306-6308 NN denotes μg
T16559 6308-6309 NN denotes /
T16560 6309-6311 NN denotes ml
T16561 6312-6321 NN denotes propidium
T16562 6322-6328 NN denotes iodide
T16563 6329-6330 -LRB- denotes (
T16564 6330-6331 NNP denotes [
T16565 6331-6333 NNP denotes PI
T16566 6333-6334 NNP denotes ]
T16567 6334-6335 : denotes ;
T16568 6336-6346 NNP denotes Calbiochem
T16569 6347-6352 NNP denotes Corp.
T16570 6352-6353 : denotes ;
T16571 6354-6356 NNP denotes La
T16572 6357-6362 NNP denotes Jolla
T16573 6362-6363 , denotes ,
T16574 6364-6366 NNP denotes CA
T16575 6366-6367 : denotes ;
T16576 6368-6369 CD denotes 5
T16577 6370-6375 NN denotes mg/ml
T16578 6375-6376 -RRB- denotes )
T16579 6377-6380 IN denotes for
T16580 6381-6384 CD denotes one
T16581 6385-6391 NN denotes minute
T16582 6392-6394 IN denotes on
T16583 6395-6398 NN denotes ice
T16584 6398-6399 . denotes .
T16585 6400-6402 IN denotes At
T16586 6403-6408 JJS denotes least
T16587 6409-6414 CD denotes 1,000
T16588 6415-6420 NNS denotes cells
T16589 6421-6424 IN denotes per
T16590 6425-6431 NN denotes sample
T16591 6432-6436 VBD denotes were
T16592 6437-6440 VBN denotes run
T16593 6441-6443 IN denotes on
T16594 6444-6445 DT denotes a
T16595 6446-6448 NNP denotes BD
T16596 6449-6460 NNP denotes FACSCalibur
T16597 6461-6465 NN denotes flow
T16598 6466-6475 NN denotes cytometer
T16599 6475-6476 . denotes .
T16653 6478-6489 NNP denotes Statistical
T16654 6490-6498 NN denotes analysis
T16655 6499-6502 DT denotes The
T16656 6503-6514 JJ denotes statistical
T16657 6515-6527 NN denotes significance
T16658 6528-6530 IN denotes of
T16659 6531-6542 NNS denotes differences
T16660 6543-6550 IN denotes between
T16661 6551-6557 NNS denotes groups
T16662 6558-6561 VBD denotes was
T16663 6562-6572 VBN denotes determined
T16664 6573-6578 VBG denotes using
T16665 6579-6582 DT denotes the
T13960 274-277 CC denotes and
T13961 278-286 NN denotes approval
T13962 287-289 IN denotes by
T13963 290-293 DT denotes the
T13964 294-302 JJ denotes regional
T13965 303-309 NNS denotes ethics
T13966 310-319 NN denotes committee
T13967 319-320 . denotes .
T13968 321-327 NNP denotes Highly
T13969 328-336 VBD denotes purified
T13970 337-344 VBG denotes resting
T13971 345-350 JJ denotes human
T13972 351-364 NNS denotes B-lymphocytes
T13973 365-366 -LRB- denotes (
T13974 366-370 CD denotes CD19
T13975 370-371 NN denotes +
T13976 372-377 NNS denotes cells
T13977 377-378 -RRB- denotes )
T13978 379-383 VBD denotes were
T13979 384-392 VBN denotes isolated
T13980 393-397 IN denotes from
T13981 398-401 DT denotes the
T13982 402-412 JJ denotes peripheral
T13983 413-418 NN denotes blood
T13984 419-421 IN denotes by
T13985 422-431 VBG denotes rosetting
T13986 432-436 IN denotes with
T13987 437-451 JJ denotes immunomagnetic
T13988 452-457 NNS denotes beads
T13989 458-459 -LRB- denotes (
T13990 459-468 NNPS denotes Dynabeads
T13991 469-473 NNP denotes M450
T13992 473-474 : denotes ;
T13993 475-480 NNP denotes Dynal
T13994 480-481 , denotes ,
T13995 482-486 NNP denotes Oslo
T13996 486-487 , denotes ,
T13997 488-494 NNP denotes Norway
T13998 494-495 -RRB- denotes )
T13999 496-498 IN denotes as
T14000 499-508 VBN denotes described
T14001 509-510 NNP denotes [
T14002 510-512 CD denotes 55
T14003 512-513 NNP denotes ]
T14861 2397-2404 NN denotes peptide
T14862 2405-2409 IN denotes from
T14863 2410-2415 NNP denotes Santa
T14864 2416-2420 NNP denotes Cruz
T14865 2421-2434 NNP denotes Biotechnology
T14866 2435-2436 -LRB- denotes (
T14867 2436-2441 NNP denotes Santa
T14868 2442-2446 NNP denotes Cruz
T14869 2446-2447 , denotes ,
T14870 2448-2451 NNP denotes San
T14871 2452-2457 NNP denotes Diego
T14872 2457-2458 , denotes ,
T14873 2459-2461 NNP denotes CA
T14874 2461-2462 , denotes ,
T14875 2463-2466 NNP denotes USA
T14876 2466-2467 -RRB- denotes )
T14877 2467-2468 . denotes .
T14878 2469-2471 IN denotes As
T14879 2472-2481 JJ denotes secondary
T14880 2482-2492 NNS denotes antibodies
T14881 2493-2499 VBD denotes served
T14882 2500-2510 JJ denotes anti-mouse
T14883 2510-2511 , denotes ,
T14884 2512-2521 JJ denotes anti-goat
T14885 2522-2524 CC denotes or
T14886 2525-2536 JJ denotes anti-rabbit
T14887 2537-2540 SYM denotes IgG
T14888 2540-2541 : denotes -
T14889 2542-2553 NN denotes horseradish
T14890 2554-2564 NN denotes peroxidase
T14891 2565-2566 -LRB- denotes (
T14892 2566-2569 NNP denotes HRP
T14893 2569-2570 -RRB- denotes )
T14894 2571-2575 IN denotes from
T14895 2576-2590 NNP denotes Dakocytomation
T14924 2738-2739 , denotes ,
T14925 2740-2754 JJ denotes anti-CD19-FITC
T14926 2754-2755 . denotes .
T14927 2756-2759 DT denotes The
T14928 2760-2770 NNS denotes antibodies
T14929 2771-2775 VBN denotes used
T14930 2776-2779 IN denotes for
T14931 2780-2784 NN denotes cell
T14932 2785-2792 VBG denotes sorting
T14933 2793-2797 VBD denotes were
T14934 2798-2807 JJ denotes anti-CD19
T14935 2808-2811 NNS denotes PC5
T14936 2812-2816 IN denotes from
T14937 2817-2827 NNP denotes Immunotech
T14938 2828-2830 NNP denotes SA
T14939 2831-2832 -LRB- denotes (
T14940 2832-2841 NNP denotes Marseille
T14941 2841-2842 , denotes ,
T14942 2843-2849 NNP denotes France
T14943 2849-2850 -RRB- denotes )
T14944 2851-2854 CC denotes and
T14945 2855-2864 JJ denotes anti-CD27
T14946 2865-2867 NN denotes PE
T14947 2868-2872 IN denotes from
T14948 2873-2879 NNP denotes Becton
T14949 2880-2889 NNP denotes Dickinson
T14950 2889-2890 , denotes ,
T14951 2891-2902 NNP denotes Biosciences
T14952 2903-2913 NNP denotes Pharmingen
T14953 2914-2915 -LRB- denotes (
T14954 2915-2918 NNP denotes San
T14955 2919-2924 NNP denotes Diego
T14956 2924-2925 , denotes ,
T14957 2926-2928 NNP denotes CA
T14958 2928-2929 , denotes ,
T16666 6583-6589 VBN denotes paired
T16667 6590-6600 JJ denotes two-tailed
T16668 6601-6609 NNP denotes Wilcoxon
T16669 6610-6623 JJ denotes nonparametric
T16670 6624-6628 NN denotes test
T16671 6628-6629 , denotes ,
T16672 6630-6632 IN denotes by
T16673 6633-6641 VBG denotes applying
T16674 6642-6648 NNP denotes SPSS10
T16675 6648-6650 CD denotes .1
T16676 6651-6659 NN denotes software
T16677 6660-6661 -LRB- denotes (
T16678 6661-6665 NNP denotes SPSS
T16679 6666-6670 NNP denotes Inc.
T16680 6670-6671 , denotes ,
T16681 6672-6679 NNP denotes Chicago
T16682 6679-6680 , denotes ,
T16683 6681-6683 NNP denotes IL
T16684 6683-6684 , denotes ,
T16685 6685-6688 NNP denotes USA
T16686 6688-6689 -RRB- denotes )
T16687 6689-6690 . denotes .
T16688 6691-6692 NNP denotes P
T16689 6693-6699 VBZ denotes values
T16690 6700-6704 JJR denotes less
T16691 6705-6709 IN denotes than
T16692 6710-6714 CD denotes 0.05
T16693 6715-6719 VBD denotes were
T16694 6720-6730 VBN denotes considered
T16695 6731-6742 JJ denotes significant
T16696 6742-6743 . denotes .
R11640 T14960 T14933 punct ),were
R11641 T14961 T14933 punct .,were
R10828 T13907 T13908 compound Cell,culture
R10829 T13908 T13908 ROOT culture,culture
R10830 T13909 T13911 mark If,specified
R10831 T13910 T13911 neg not,specified
R10832 T13911 T13916 advcl specified,cultured
R10833 T13912 T13916 punct ",",cultured
R10834 T13913 T13914 det all,cells
R10835 T13914 T13916 nsubjpass cells,cultured
R10836 T13915 T13916 auxpass were,cultured
R10837 T13916 T13916 ROOT cultured,cultured
R10838 T13917 T13916 prep in,cultured
R10839 T13918 T13920 nmod X-VIVO,™
R10840 T13919 T13920 nummod 15,™
R10841 T13920 T13929 nmod ™,medium
R10842 T13921 T13922 punct (,BioWhittaker
R10843 T13922 T13920 appos BioWhittaker,™
R10844 T13923 T13922 punct ",",BioWhittaker
R10845 T13924 T13922 conj Verviers,BioWhittaker
R10846 T13925 T13924 punct ",",Verviers
R10847 T13926 T13924 conj Belgium,Verviers
R10848 T13927 T13922 punct ),BioWhittaker
R10849 T13928 T13929 amod serum-free,medium
R10850 T13929 T13916 dobj medium,cultured
R10851 T13930 T13916 prep at,cultured
R10852 T13931 T13932 nummod 37,°
R10853 T13932 T13933 nummod °,C
R10854 T13933 T13930 pobj C,at
R10855 T13934 T13916 cc and,cultured
R10856 T13935 T13936 nummod 5,%
R10857 T13936 T13916 conj %,cultured
R10858 T13937 T13936 nummod CO2,%
R10859 T13938 T13937 prep in,CO2
R10860 T13939 T13938 pobj air,in
R10861 T13940 T13937 punct .,CO2
R10862 T13941 T13942 amod Peripheral,blood
R10863 T13942 T13944 nsubjpass blood,provided
R10864 T13943 T13944 auxpass was,provided
R10865 T13944 T13944 ROOT provided,provided
R10866 T13945 T13944 agent by,provided
R10867 T13946 T13948 det the,Bank
R10868 T13947 T13948 compound Blood,Bank
R10869 T13948 T13945 pobj Bank,by
R10870 T13949 T13948 prep at,Bank
R10871 T13950 T13952 compound Buskerud,Hospital
R10872 T13951 T13952 compound Regional,Hospital
R10873 T13952 T13949 pobj Hospital,at
R10874 T13953 T13944 prep with,provided
R10875 T13954 T13955 amod formal,agreement
R10876 T13955 T13953 pobj agreement,with
R10877 T13956 T13955 prep by,agreement
R10878 T13957 T13958 det the,patients
R10879 T13958 T13956 pobj patients,by
R10880 T13959 T13944 punct ",",provided
R10881 T13960 T13944 cc and,provided
R10882 T13961 T13944 conj approval,provided
R10883 T13962 T13961 prep by,approval
R10884 T13963 T13966 det the,committee
R10885 T13964 T13966 amod regional,committee
R10886 T13965 T13966 compound ethics,committee
R10887 T13966 T13962 pobj committee,by
R10888 T13967 T13944 punct .,provided
R10889 T13968 T13969 nsubj Highly,purified
R10890 T13969 T13969 ROOT purified,purified
R10891 T13970 T13969 xcomp resting,purified
R10892 T13971 T13972 amod human,B-lymphocytes
R10893 T13972 T13970 dobj B-lymphocytes,resting
R10894 T13973 T13972 punct (,B-lymphocytes
R10895 T13974 T13976 nummod CD19,cells
R10896 T13975 T13976 compound +,cells
R10897 T13976 T13972 appos cells,B-lymphocytes
R10898 T13977 T13972 punct ),B-lymphocytes
R10899 T13978 T13979 auxpass were,isolated
R10900 T13979 T13969 conj isolated,purified
R10901 T13980 T13979 prep from,isolated
R10902 T13981 T13983 det the,blood
R10903 T13982 T13983 amod peripheral,blood
R10904 T13983 T13980 pobj blood,from
R10905 T13984 T13979 prep by,isolated
R10906 T13985 T13984 pcomp rosetting,by
R10907 T13986 T13985 prep with,rosetting
R10908 T13987 T13988 amod immunomagnetic,beads
R10909 T13988 T13986 pobj beads,with
R10910 T13989 T13991 punct (,M450
R10911 T13990 T13991 compound Dynabeads,M450
R10912 T13991 T13988 appos M450,beads
R10913 T13992 T13991 punct ;,M450
R10914 T13993 T13991 conj Dynal,M450
R10915 T13994 T13993 punct ",",Dynal
R10916 T13995 T13993 conj Oslo,Dynal
R10917 T13996 T13995 punct ",",Oslo
R10918 T13997 T13995 conj Norway,Oslo
R10919 T13998 T13988 punct ),beads
R10920 T13999 T14000 mark as,described
R10921 T14000 T13985 advcl described,rosetting
R10922 T14001 T14003 nmod [,]
R10923 T14002 T14003 nummod 55,]
R10924 T14003 T14000 dobj ],described
R10925 T14004 T13969 punct .,purified
R10926 T14005 T14006 det This,procedure
R10927 T14006 T14007 nsubj procedure,yields
R10928 T14007 T14007 ROOT yields,yields
R10929 T14008 T14010 amod less,0.5
R10930 T14009 T14010 quantmod than,0.5
R10931 T14010 T14011 nummod 0.5,%
R10932 T14011 T14013 compound %,cells
R10933 T14012 T14013 compound T,cells
R10934 T14013 T14007 dobj cells,yields
R10935 T14014 T14013 punct ",",cells
R10936 T14015 T14016 nummod 0.1,%
R10937 T14016 T14018 compound %,cells
R10938 T14017 T14018 compound NK,cells
R10939 T14018 T14013 conj cells,cells
R10940 T14019 T14018 punct ",",cells
R10941 T14020 T14018 cc and,cells
R10942 T14021 T14022 nummod 0.5,%
R10943 T14022 T14023 compound %,monocytes
R10944 T14023 T14018 conj monocytes,cells
R10945 T14024 T14025 mark as,judged
R10946 T14025 T14007 advcl judged,yields
R10947 T14026 T14025 agent by,judged
R10948 T14027 T14029 amod indirect,staining
R10949 T14028 T14029 compound immunofluorescence,staining
R10950 T14029 T14026 pobj staining,by
R10951 T14030 T14007 punct .,yields
R10952 T14031 T14034 det The,lines
R10953 T14032 T14034 amod following,lines
R10954 T14033 T14034 compound cell,lines
R10955 T14034 T14040 nsubjpass lines,maintained
R10956 T14035 T14034 prep from,lines
R10957 T14036 T14038 amod human,malignancies
R10958 T14037 T14038 amod lymphoid,malignancies
R10959 T14038 T14035 pobj malignancies,from
R10960 T14039 T14040 auxpass were,maintained
R10961 T14040 T14040 ROOT maintained,maintained
R10962 T14041 T14040 prep in,maintained
R10963 T14042 T14047 nmod RPMI,GmbH
R10964 T14043 T14042 nummod 1640,RPMI
R10965 T14044 T14047 punct (,GmbH
R10966 T14045 T14047 compound PAA,GmbH
R10967 T14046 T14047 compound Laboratories,GmbH
R10968 T14047 T14041 pobj GmbH,in
R10969 T14048 T14047 punct ",",GmbH
R10970 T14049 T14047 conj Pasching,GmbH
R10971 T14050 T14049 punct ",",Pasching
R10972 T14051 T14049 npadvmod Austria,Pasching
R10973 T14052 T14047 punct ),GmbH
R10974 T14053 T14040 conj supplemented,maintained
R10975 T14054 T14053 prep with,supplemented
R10976 T14055 T14056 nummod 10,%
R10977 T14056 T14059 nmod %,serum
R10978 T14057 T14059 amod foetal,serum
R10979 T14058 T14059 amod bovine,serum
R10980 T14059 T14054 pobj serum,with
R10981 T14060 T14059 punct (,serum
R10982 T14061 T14059 appos FCS,serum
R10983 T14062 T14059 punct ),serum
R10984 T14063 T14059 punct ",",serum
R10985 T14064 T14067 nummod 100,G
R10986 T14065 T14067 compound units/ml,G
R10987 T14066 T14067 compound penicillin,G
R10988 T14067 T14059 appos G,serum
R10989 T14068 T14059 punct ",",serum
R10990 T14069 T14059 cc and,serum
R10991 T14070 T14071 nummod 100,units/ml
R10992 T14071 T14059 conj units/ml,serum
R10993 T14072 T14071 prep of,units/ml
R10994 T14073 T14074 compound streptomycin,sulphate
R10995 T14074 T14072 pobj sulphate,of
R10996 T14075 T14071 punct ",",units/ml
R10997 T14076 T14071 cc but,units/ml
R10998 T14077 T14071 conj serum-starved,units/ml
R10999 T14078 T14077 prep for,serum-starved
R11000 T14079 T14080 advmod at,least
R11001 T14080 T14081 advmod least,four
R11002 T14081 T14082 nummod four,hours
R11003 T14082 T14078 pobj hours,for
R11004 T14083 T14077 cc and,serum-starved
R11005 T14084 T14077 conj cultured,serum-starved
R11006 T14085 T14084 prep in,cultured
R11007 T14086 T14088 nsubj X-VIVO,™
R11008 T14087 T14088 nummod 15,™
R11009 T14088 T14085 pobj ™,in
R11010 T14089 T14090 advmod when,included
R11011 T14090 T14053 advcl included,supplemented
R11012 T14091 T14090 prep in,included
R11013 T14092 T14091 pobj experiments,in
R11014 T14093 T14097 punct :,lines
R11015 T14094 T14097 amod EBV-negative,lines
R11016 T14095 T14097 compound BL,lines
R11017 T14096 T14097 compound cell,lines
R11018 T14097 T14098 compound lines,Ramos
R11019 T14098 T14040 npadvmod Ramos,maintained
R11020 T14099 T14100 punct (,ECACC
R11021 T14100 T14098 appos ECACC,Ramos
R11022 T14101 T14100 nummod 85030802,ECACC
R11023 T14102 T14098 punct ),Ramos
R11024 T14103 T14098 punct ",",Ramos
R11025 T14104 T14098 conj HL60,Ramos
R11026 T14105 T14104 punct (,HL60
R11027 T14106 T14104 appos JCRB0085,HL60
R11028 T14107 T14104 punct ),HL60
R11029 T14108 T14040 punct .,maintained
R11030 T14167 T14168 compound Growth,factors/supplements
R11031 T14168 T14168 ROOT factors/supplements,factors/supplements
R11032 T14169 T14171 det The,reagents
R11033 T14170 T14171 amod following,reagents
R11034 T14171 T14173 nsubjpass reagents,used
R11035 T14172 T14173 auxpass were,used
R11036 T14173 T14263 ccomp used,obtained
R11037 T14174 T14173 prep at,used
R11038 T14175 T14176 amod indicated,concentrations
R11039 T14176 T14174 pobj concentrations,at
R11040 T14177 T14176 punct :,concentrations
R11041 T14178 T14179 compound recombinant,human
R11042 T14179 T14176 appos human,concentrations
R11043 T14180 T14181 punct (,rhu
R11044 T14181 T14179 appos rhu,human
R11045 T14182 T14176 punct ),concentrations
R11046 T14183 T14230 nsubjpass BMP-6,purchased
R11047 T14184 T14188 punct (,ml
R11048 T14185 T14186 nummod 1,μg
R11049 T14186 T14188 compound μg,ml
R11050 T14187 T14188 compound /,ml
R11051 T14188 T14183 appos ml,BMP-6
R11052 T14189 T14188 punct ",",ml
R11053 T14190 T14192 mark if,specified
R11054 T14191 T14192 neg not,specified
R11055 T14192 T14183 advcl specified,BMP-6
R11056 T14193 T14192 advmod otherwise,specified
R11057 T14194 T14192 punct ),specified
R11058 T14195 T14183 punct ",",BMP-6
R11059 T14196 T14197 nmod rhu,BMP-RIB/ALK
R11060 T14197 T14201 nmod BMP-RIB/ALK,Chimera
R11061 T14198 T14199 compound -6,/
R11062 T14199 T14201 compound /,Chimera
R11063 T14200 T14201 compound Fc,Chimera
R11064 T14201 T14230 nsubjpass Chimera,purchased
R11065 T14202 T14206 punct (,ml
R11066 T14203 T14204 nummod 5,μg
R11067 T14204 T14206 compound μg,ml
R11068 T14205 T14206 compound /,ml
R11069 T14206 T14201 appos ml,Chimera
R11070 T14207 T14206 punct ),ml
R11071 T14208 T14201 punct ",",Chimera
R11072 T14209 T14211 compound rhu,Chimera
R11073 T14210 T14211 compound BMPR-II/Fc,Chimera
R11074 T14211 T14201 appos Chimera,Chimera
R11075 T14212 T14216 punct (,ml
R11076 T14213 T14214 nummod 5,μg
R11077 T14214 T14216 nmod μg,ml
R11078 T14215 T14216 compound /,ml
R11079 T14216 T14211 parataxis ml,Chimera
R11080 T14217 T14216 punct ),ml
R11081 T14218 T14211 punct ",",Chimera
R11082 T14219 T14211 cc and,Chimera
R11083 T14220 T14221 compound recombinant,mouse
R11084 T14221 T14222 compound mouse,Noggin
R11085 T14222 T14211 conj Noggin,Chimera
R11086 T14223 T14227 punct (,ml
R11087 T14224 T14225 nummod 5,μg
R11088 T14225 T14227 nmod μg,ml
R11089 T14226 T14227 compound /,ml
R11090 T14227 T14222 appos ml,Noggin
R11091 T14228 T14222 punct ),Noggin
R11092 T14229 T14230 auxpass were,purchased
R11093 T14230 T14173 conj purchased,used
R11094 T14231 T14230 prep from,purchased
R11095 T14232 T14233 compound R&D,Systems
R11096 T14233 T14231 pobj Systems,from
R11097 T14234 T14233 punct (,Systems
R11098 T14235 T14233 npadvmod Abingdon,Systems
R11099 T14236 T14235 punct ",",Abingdon
R11100 T14237 T14235 appos UK,Abingdon
R11101 T14238 T14233 punct ),Systems
R11102 T14239 T14263 punct ;,obtained
R11103 T14240 T14241 compound Anti-IgM,F
R11104 T14241 T14263 nsubjpass F,obtained
R11105 T14242 T14243 punct (,ab
R11106 T14243 T14241 appos ab,F
R11107 T14244 T14245 punct ),2
R11108 T14245 T14246 nummod 2,fragments
R11109 T14246 T14241 dobj fragments,F
R11110 T14247 T14246 prep of,fragments
R11111 T14248 T14250 compound rabbit,antibodies
R11112 T14249 T14250 compound polyclonal,antibodies
R11113 T14250 T14247 pobj antibodies,of
R11114 T14251 T14263 prep to,obtained
R11115 T14252 T14255 amod human,chain
R11116 T14253 T14255 compound IgM,chain
R11117 T14254 T14255 compound heavy,chain
R11118 T14255 T14251 pobj chain,to
R11119 T14256 T14255 punct (,chain
R11120 T14257 T14258 nummod 37.5,μg
R11121 T14258 T14260 nmod μg,ml
R11122 T14259 T14260 compound /,ml
R11123 T14260 T14255 appos ml,chain
R11124 T14261 T14260 punct ),ml
R11125 T14262 T14263 auxpass was,obtained
R11126 T14263 T14263 ROOT obtained,obtained
R11127 T14264 T14263 prep from,obtained
R11128 T14265 T14264 pobj Dako,from
R11129 T14266 T14265 punct ",",Dako
R11130 T14267 T14265 conj Copenhagen,Dako
R11131 T14268 T14267 punct ",",Copenhagen
R11132 T14269 T14267 conj Denmark,Copenhagen
R11133 T14270 T14269 cc and,Denmark
R11134 T14271 T14273 compound rhu,ligand
R11135 T14272 T14273 compound CD40,ligand
R11136 T14273 T14269 conj ligand,Denmark
R11137 T14274 T14275 punct (,CD40L
R11138 T14275 T14273 appos CD40L,ligand
R11139 T14276 T14275 punct ",",CD40L
R11140 T14277 T14278 nummod 10,ng/ml
R11141 T14278 T14275 appos ng/ml,CD40L
R11142 T14279 T14275 punct ),CD40L
R11143 T14280 T14263 auxpass was,obtained
R11144 T14281 T14282 det a,gift
R11145 T14282 T14263 dobj gift,obtained
R11146 T14283 T14263 prep from,obtained
R11147 T14284 T14285 compound Immunex,Corp.
R11148 T14285 T14283 pobj Corp.,from
R11149 T14286 T14285 punct (,Corp.
R11150 T14287 T14285 appos Seattle,Corp.
R11151 T14288 T14287 punct ",",Seattle
R11152 T14289 T14287 conj WA,Seattle
R11153 T14290 T14285 punct ),Corp.
R11154 T14291 T14263 punct .,obtained
R11398 T14718 T14719 nsubj Antibodies,used
R11399 T14719 T14719 ROOT used,used
R11400 T14720 T14719 prep for,used
R11401 T14721 T14720 pobj flow,for
R11402 T14722 T14723 amod cytometric,analysis
R11403 T14723 T14720 pobj analysis,for
R11404 T14724 T14723 cc and,analysis
R11405 T14725 T14727 compound immunoblot,Antibodies
R11406 T14726 T14727 compound analysis,Antibodies
R11407 T14727 T14723 conj Antibodies,analysis
R11408 T14728 T14723 prep against,analysis
R11409 T14729 T14732 det the,Act-RIA
R11410 T14730 T14731 amod human,BMP-receptors
R11411 T14731 T14732 compound BMP-receptors,Act-RIA
R11412 T14732 T14728 pobj Act-RIA,against
R11413 T14733 T14732 punct ",",Act-RIA
R11414 T14734 T14732 conj BMP-RIB,Act-RIA
R11415 T14735 T14734 punct ",",BMP-RIB
R11416 T14736 T14734 conj BMPR-II,BMP-RIB
R11417 T14737 T14736 punct ",",BMPR-II
R11418 T14738 T14736 conj Act-RIIA,BMPR-II
R11419 T14739 T14738 cc and,Act-RIIA
R11420 T14740 T14738 conj Act-RIIb,Act-RIIA
R11421 T14741 T14742 auxpass were,purchased
R11422 T14742 T14719 conj purchased,used
R11423 T14743 T14742 prep from,purchased
R11424 T14744 T14743 pobj R,from
R11425 T14745 T14744 cc &,R
R11426 T14746 T14747 compound D,Systems
R11427 T14747 T14744 conj Systems,R
R11428 T14748 T14747 punct (,Systems
R11429 T14749 T14747 npadvmod Abingdon,Systems
R11430 T14750 T14749 punct ",",Abingdon
R11431 T14751 T14749 appos UK,Abingdon
R11432 T14752 T14749 punct ),Abingdon
R11433 T14753 T14719 punct .,used
R11434 T14754 T14760 nsubjpass Detection,tried
R11435 T14755 T14754 prep of,Detection
R11436 T14756 T14757 det the,BMP-6-protein
R11437 T14757 T14755 pobj BMP-6-protein,of
R11438 T14758 T14760 aux has,tried
R11439 T14759 T14760 auxpass been,tried
R11440 T14760 T14766 ccomp tried,goat
R11441 T14761 T14760 prep with,tried
R11442 T14762 T14764 det the,antibodies
R11443 T14763 T14764 amod following,antibodies
R11444 T14764 T14761 pobj antibodies,with
R11445 T14765 T14766 punct :,goat
R11446 T14766 T14766 ROOT goat,goat
R11447 T14767 T14768 amod polyclonal,anti-BMP-6
R11448 T14768 T14766 dobj anti-BMP-6,goat
R11449 T14769 T14771 punct (,Cruz
R11450 T14770 T14771 compound Santa,Cruz
R11451 T14771 T14766 npadvmod Cruz,goat
R11452 T14772 T14771 punct ",",Cruz
R11453 T14773 T14774 compound San,Diego
R11454 T14774 T14771 npadvmod Diego,Cruz
R11455 T14775 T14774 punct ",",Diego
R11456 T14776 T14774 conj CA,Diego
R11457 T14777 T14776 punct ",",CA
R11458 T14778 T14776 conj USA,CA
R11459 T14779 T14776 punct ),CA
R11460 T14780 T14771 punct ",",Cruz
R11461 T14781 T14782 amod monoclonal,mouse
R11462 T14782 T14783 compound mouse,anti-BMP-6
R11463 T14783 T14771 appos anti-BMP-6,Cruz
R11464 T14784 T14783 cc and,anti-BMP-6
R11465 T14785 T14787 amod polyclonal,anti-BMP-6
R11466 T14786 T14787 compound goat,anti-BMP-6
R11467 T14787 T14783 conj anti-BMP-6,anti-BMP-6
R11468 T14788 T14787 prep from,anti-BMP-6
R11469 T14789 T14788 pobj R,from
R11470 T14790 T14789 cc &,R
R11471 T14791 T14792 compound D,Systems
R11472 T14792 T14789 conj Systems,R
R11473 T14793 T14792 punct (,Systems
R11474 T14794 T14792 npadvmod Abingdon,Systems
R11475 T14795 T14794 punct ",",Abingdon
R11476 T14796 T14794 appos UK,Abingdon
R11477 T14797 T14794 punct ),Abingdon
R11478 T14798 T14792 punct ",",Systems
R11479 T14799 T14787 cc and,anti-BMP-6
R11480 T14800 T14802 nmod mouse,anti-BMP-6
R11481 T14801 T14802 amod monoclonal,anti-BMP-6
R11482 T14802 T14787 conj anti-BMP-6,anti-BMP-6
R11483 T14803 T14806 punct (,Inc
R11484 T14804 T14806 compound Chemicon,Inc
R11485 T14805 T14806 compound International,Inc
R11486 T14806 T14771 appos Inc,Cruz
R11487 T14807 T14806 punct ",",Inc
R11488 T14808 T14806 conj Temecula,Inc
R11489 T14809 T14808 punct ",",Temecula
R11490 T14810 T14808 conj CA,Temecula
R11491 T14811 T14810 punct ",",CA
R11492 T14812 T14810 conj USA,CA
R11493 T14813 T14806 punct ),Inc
R11494 T14814 T14766 punct .,goat
R11495 T14815 T14820 nsubjpass Characterisation,done
R11496 T14816 T14815 prep of,Characterisation
R11497 T14817 T14818 amod BMP-signalling,pathways
R11498 T14818 T14816 pobj pathways,of
R11499 T14819 T14820 auxpass was,done
R11500 T14820 T14820 ROOT done,done
R11501 T14821 T14820 agent by,done
R11502 T14822 T14821 pobj use,by
R11503 T14823 T14822 prep of,use
R11504 T14824 T14823 pobj anti-phospho-Smad1,of
R11505 T14825 T14824 punct ",",anti-phospho-Smad1
R11506 T14826 T14824 nummod -5,anti-phospho-Smad1
R11507 T14827 T14824 punct ",",anti-phospho-Smad1
R11508 T14828 T14820 punct -,done
R11509 T14829 T14820 cc and,done
R11510 T14830 T14832 nummod 8,antibody
R11511 T14831 T14832 amod polyclonal,antibody
R11512 T14832 T14820 conj antibody,done
R11513 T14833 T14832 punct (,antibody
R11514 T14834 T14832 appos Chemicon,antibody
R11515 T14835 T14834 punct ",",Chemicon
R11516 T14836 T14834 conj Temecula,Chemicon
R11517 T14837 T14836 punct ",",Temecula
R11518 T14838 T14836 conj CA,Temecula
R11519 T14839 T14838 punct ",",CA
R11520 T14840 T14838 conj USA,CA
R11521 T14841 T14832 punct ),antibody
R11522 T14842 T14820 punct .,done
R11523 T14843 T14844 compound Expression,levels
R11524 T14844 T14850 nsubjpass levels,detected
R11525 T14845 T14844 prep of,levels
R11526 T14846 T14845 pobj Id1,of
R11527 T14847 T14848 nummod 3,proteins
R11528 T14848 T14850 nsubjpass proteins,detected
R11529 T14849 T14850 auxpass were,detected
R11530 T14850 T14850 ROOT detected,detected
R11531 T14851 T14850 prep with,detected
R11532 T14852 T14854 amod polyclonal,antibody
R11533 T14853 T14854 compound rabbit,antibody
R11534 T14854 T14851 pobj antibody,with
R11535 T14855 T14854 cc and,antibody
R11536 T14856 T14854 conj detection,antibody
R11537 T14857 T14858 auxpass was,blocked
R11538 T14858 T14850 conj blocked,detected
R11539 T14859 T14858 prep with,blocked
R11540 T14860 T14859 pcomp blocking,with
R11541 T14861 T14860 dobj peptide,blocking
R11542 T14862 T14860 prep from,blocking
R11543 T14863 T14865 compound Santa,Biotechnology
R11544 T14864 T14865 compound Cruz,Biotechnology
R11545 T14865 T14862 pobj Biotechnology,from
R11546 T14866 T14865 punct (,Biotechnology
R11547 T14867 T14868 compound Santa,Cruz
R11548 T14868 T14865 appos Cruz,Biotechnology
R11549 T14869 T14865 punct ",",Biotechnology
R11550 T14870 T14871 compound San,Diego
R11551 T14871 T14865 appos Diego,Biotechnology
R11552 T14872 T14865 punct ",",Biotechnology
R11553 T14873 T14865 appos CA,Biotechnology
R11554 T14874 T14873 punct ",",CA
R11555 T14875 T14865 appos USA,Biotechnology
R11556 T14876 T14865 punct ),Biotechnology
R11557 T14877 T14850 punct .,detected
R11558 T14878 T14881 mark As,served
R11559 T14879 T14880 amod secondary,antibodies
R11560 T14880 T14881 nsubj antibodies,served
R11561 T14881 T14881 ROOT served,served
R11562 T14882 T14887 acomp anti-mouse,IgG
R11563 T14883 T14882 punct ",",anti-mouse
R11564 T14884 T14882 conj anti-goat,anti-mouse
R11565 T14885 T14884 cc or,anti-goat
R11566 T14886 T14884 conj anti-rabbit,anti-goat
R11567 T14887 T14881 dobj IgG,served
R11568 T14888 T14887 punct -,IgG
R11569 T14889 T14890 compound horseradish,peroxidase
R11570 T14890 T14887 appos peroxidase,IgG
R11571 T14891 T14892 punct (,HRP
R11572 T14892 T14890 appos HRP,peroxidase
R11573 T14893 T14892 punct ),HRP
R11574 T14894 T14892 prep from,HRP
R11575 T14895 T14896 compound Dakocytomation,AS
R11576 T14896 T14894 pobj AS,from
R11577 T14897 T14896 punct (,AS
R11578 T14898 T14896 npadvmod Copenhagen,AS
R11579 T14899 T14898 punct ",",Copenhagen
R11580 T14900 T14898 appos Denmark,Copenhagen
R11581 T14901 T14896 punct ),AS
R11582 T14902 T14881 prep for,served
R11583 T14903 T14904 compound immunoblot,analysis
R11584 T14904 T14902 pobj analysis,for
R11585 T14905 T14881 punct .,served
R11586 T14906 T14907 nsubj Anti-β-actin,was
R11587 T14907 T14907 ROOT was,was
R11588 T14908 T14907 prep from,was
R11589 T14909 T14908 pobj Santa-Cruz,from
R11590 T14910 T14907 punct .,was
R11591 T14911 T14922 prep From,purchased
R11592 T14912 T14913 compound Becton,Dickinson
R11593 T14913 T14911 pobj Dickinson,From
R11594 T14914 T14913 punct (,Dickinson
R11595 T14915 T14916 compound San,Jose
R11596 T14916 T14913 appos Jose,Dickinson
R11597 T14917 T14916 punct ",",Jose
R11598 T14918 T14913 conj CA,Dickinson
R11599 T14919 T14913 punct ),Dickinson
R11600 T14920 T14922 punct ",",purchased
R11601 T14921 T14922 nsubj we,purchased
R11602 T14922 T14922 ROOT purchased,purchased
R11603 T14923 T14925 amod anti-CD19-PE,anti-CD19-FITC
R11604 T14924 T14925 punct ",",anti-CD19-FITC
R11605 T14925 T14922 dobj anti-CD19-FITC,purchased
R11606 T14926 T14922 punct .,purchased
R11607 T14927 T14928 det The,antibodies
R11608 T14928 T14933 nsubj antibodies,were
R11609 T14929 T14928 acl used,antibodies
R11610 T14930 T14929 prep for,used
R11611 T14931 T14930 pobj cell,for
R11612 T14932 T14928 acl sorting,antibodies
R11613 T14933 T14933 ROOT were,were
R11614 T14934 T14935 amod anti-CD19,PC5
R11615 T14935 T14933 attr PC5,were
R11616 T14936 T14935 prep from,PC5
R11617 T14937 T14938 compound Immunotech,SA
R11618 T14938 T14936 pobj SA,from
R11619 T14939 T14938 punct (,SA
R11620 T14940 T14938 npadvmod Marseille,SA
R11621 T14941 T14940 punct ",",Marseille
R11622 T14942 T14940 conj France,Marseille
R11623 T14943 T14942 punct ),France
R11624 T14944 T14938 cc and,SA
R11625 T14945 T14946 amod anti-CD27,PE
R11626 T14946 T14938 conj PE,SA
R11627 T14947 T14946 prep from,PE
R11628 T14948 T14949 compound Becton,Dickinson
R11629 T14949 T14947 pobj Dickinson,from
R11630 T14950 T14949 punct ",",Dickinson
R11631 T14951 T14952 compound Biosciences,Pharmingen
R11632 T14952 T14949 conj Pharmingen,Dickinson
R11633 T14953 T14955 punct (,Diego
R11634 T14954 T14955 compound San,Diego
R11635 T14955 T14949 appos Diego,Dickinson
R11636 T14956 T14955 punct ",",Diego
R11637 T14957 T14955 conj CA,Diego
R11638 T14958 T14957 punct ",",CA
R11639 T14959 T14957 conj USA,CA
R11699 T15151 T15154 nsubj Cell,purified
R11700 T15152 T15154 advcl sorting,purified
R11701 T15153 T15154 nsubj Highly,purified
R11702 T15154 T15154 ROOT purified,purified
R11703 T15155 T15166 nsubjpass CD19,obtained
R11704 T15156 T15166 npadvmod +,obtained
R11705 T15157 T15166 nsubjpass CD27,obtained
R11706 T15158 T15157 punct -,CD27
R11707 T15159 T15157 cc or,CD27
R11708 T15160 T15164 nummod CD19,cells
R11709 T15161 T15164 nummod +,cells
R11710 T15162 T15164 compound CD27,cells
R11711 T15163 T15164 compound +,cells
R11712 T15164 T15166 nsubjpass cells,obtained
R11713 T15165 T15166 auxpass were,obtained
R11714 T15166 T15154 ccomp obtained,purified
R11715 T15167 T15166 prep by,obtained
R11716 T15168 T15167 pcomp staining,by
R11717 T15169 T15168 dobj CD19,staining
R11718 T15170 T15171 nummod +,cells
R11719 T15171 T15169 appos cells,CD19
R11720 T15172 T15171 prep with,cells
R11721 T15173 T15174 amod anti-CD27,PE
R11722 T15174 T15172 pobj PE,with
R11723 T15175 T15174 cc and,PE
R11724 T15176 T15178 compound CD19,mAbs
R11725 T15177 T15178 compound PC5,mAbs
R11726 T15178 T15174 conj mAbs,PE
R11727 T15179 T15168 prep for,staining
R11728 T15180 T15181 nummod 30,minutes
R11729 T15181 T15179 pobj minutes,for
R11730 T15182 T15181 prep at,minutes
R11731 T15183 T15184 nummod 4,°
R11732 T15184 T15185 nummod °,C
R11733 T15185 T15182 pobj C,at
R11734 T15186 T15166 punct ",",obtained
R11735 T15187 T15166 advcl followed,obtained
R11736 T15188 T15187 prep by,followed
R11737 T15189 T15188 pcomp washing,by
R11738 T15190 T15189 prep with,washing
R11739 T15191 T15190 pobj PBS,with
R11740 T15192 T15189 cc and,washing
R11741 T15193 T15189 conj sorting,washing
R11742 T15194 T15193 prep on,sorting
R11743 T15195 T15196 compound FACS,DiVa
R11744 T15196 T15194 pobj DiVa,on
R11745 T15197 T15196 prep from,DiVa
R11746 T15198 T15199 compound Becton,Dickinson
R11747 T15199 T15197 pobj Dickinson,from
R11748 T15200 T15154 punct .,purified
R12228 T15878 T15888 nmod Analysis,expression
R12229 T15879 T15878 prep of,Analysis
R12230 T15880 T15882 amod BMP-6,RNA
R12231 T15881 T15882 compound messenger,RNA
R12232 T15882 T15879 pobj RNA,of
R12233 T15883 T15882 punct (,RNA
R12234 T15884 T15882 appos mRNA,RNA
R12235 T15885 T15882 punct ),RNA
R12236 T15886 T15888 nmod expression,expression
R12237 T15887 T15888 amod Endogenous,expression
R12238 T15888 T15894 nsubjpass expression,examined
R12239 T15889 T15888 prep of,expression
R12240 T15890 T15892 det the,gene
R12241 T15891 T15892 compound BMP-6,gene
R12242 T15892 T15889 pobj gene,of
R12243 T15893 T15894 auxpass was,examined
R12244 T15894 T15894 ROOT examined,examined
R12245 T15895 T15894 agent by,examined
R12246 T15896 T15899 amod reverse,reaction
R12247 T15897 T15899 compound transcription-polymerase,reaction
R12248 T15898 T15899 compound chain,reaction
R12249 T15899 T15895 pobj reaction,by
R12250 T15900 T15894 punct .,examined
R12251 T15901 T15902 amod Total,RNA
R12252 T15902 T15904 nsubjpass RNA,isolated
R12253 T15903 T15904 auxpass was,isolated
R12254 T15904 T15904 ROOT isolated,isolated
R12255 T15905 T15904 advcl using,isolated
R12256 T15906 T15907 advmod Absolutely,RNA
R12257 T15907 T15920 nmod RNA,according
R12258 T15908 T15911 nummod ™,Kit
R12259 T15909 T15911 compound RT-PCR,Kit
R12260 T15910 T15911 compound Miniprep,Kit
R12261 T15911 T15918 npadvmod Kit,Netherland
R12262 T15912 T15911 punct (,Kit
R12263 T15913 T15914 compound Stratagene,Europe
R12264 T15914 T15911 appos Europe,Kit
R12265 T15915 T15914 punct ",",Europe
R12266 T15916 T15914 conj Amsterdam,Europe
R12267 T15917 T15916 punct ",",Amsterdam
R12268 T15918 T15907 advmod Netherland,RNA
R12269 T15919 T15907 punct ),RNA
R12270 T15920 T15905 dobj according,using
R12271 T15921 T15920 prep to,according
R12272 T15922 T15924 det the,instructions
R12273 T15923 T15924 compound manufacturers,instructions
R12274 T15924 T15921 pobj instructions,to
R12275 T15925 T15904 punct .,isolated
R12276 T15926 T15933 nsubjpass Quantification,achieved
R12277 T15927 T15926 prep of,Quantification
R12278 T15928 T15931 det the,RNA
R12279 T15929 T15931 amod isolated,RNA
R12280 T15930 T15931 amod total,RNA
R12281 T15931 T15927 pobj RNA,of
R12282 T15932 T15933 auxpass was,achieved
R12283 T15933 T15933 ROOT achieved,achieved
R12284 T15934 T15933 prep by,achieved
R12285 T15935 T15934 pcomp using,by
R12286 T15936 T15937 compound spectrophotometric,OD260
R12287 T15937 T15938 compound OD260,measurements
R12288 T15938 T15935 dobj measurements,using
R12289 T15939 T15933 punct .,achieved
R12290 T15940 T15941 compound Equal,amounts
R12291 T15941 T15947 nsubjpass amounts,transcribed
R12292 T15942 T15941 prep of,amounts
R12293 T15943 T15942 pobj RNA,of
R12294 T15944 T15947 auxpass were,transcribed
R12295 T15945 T15946 advmod then,reverse
R12297 T15947 T15947 ROOT transcribed,transcribed
R12298 T15948 T15949 aux to,cDNA
R12299 T15949 T15947 advcl cDNA,transcribed
R12300 T15950 T15949 prep with,cDNA
R12301 T15951 T15952 compound TaqMan,®
R12302 T15952 T15950 pobj ®,with
R12303 T15953 T15953 ROOT Reverse,Reverse
R12304 T15954 T15955 compound Transcription,Reagents
R12305 T15955 T15953 dobj Reagents,Reverse
R12306 T15956 T15958 punct (,Biosystems
R12307 T15957 T15958 compound Applied,Biosystems
R12308 T15958 T15955 appos Biosystems,Reagents
R12309 T15959 T15958 punct ",",Biosystems
R12310 T15960 T15961 compound Foster,City
R12311 T15961 T15958 npadvmod City,Biosystems
R12312 T15962 T15961 punct ",",City
R12313 T15963 T15961 conj CA,City
R12314 T15964 T15963 punct ",",CA
R12315 T15965 T15963 conj U.S.A,CA
R12316 T15966 T15958 punct ),Biosystems
R12317 T15967 T15953 punct .,Reverse
R12318 T15968 T15969 aux To,measure
R12319 T15969 T15981 advcl measure,carried
R12320 T15970 T15971 compound mRNA,expression
R12321 T15971 T15981 nsubjpass expression,carried
R12322 T15972 T15971 prep of,expression
R12323 T15973 T15972 pobj BMP6,of
R12324 T15974 T15973 punct ",",BMP6
R12325 T15975 T15973 conj Id1,BMP6
R12326 T15976 T15971 appos Id4,expression
R12327 T15977 T15976 cc and,Id4
R12328 T15978 T15979 compound PGK1,PCR
R12329 T15979 T15976 conj PCR,Id4
R12330 T15980 T15981 auxpass were,carried
R12331 T15981 T15981 ROOT carried,carried
R12332 T15982 T15981 prt out,carried
R12333 T15983 T15981 prep with,carried
R12334 T15984 T15985 compound TaqMan,®
R12335 T15985 T15981 advcl ®,carried
R12336 T15986 T15988 amod universal,mix
R12337 T15987 T15988 compound master,mix
R12338 T15988 T15985 dobj mix,®
R12339 T15989 T15985 punct .,®
R12340 T15990 T15994 nsubjpass Primers,provided
R12341 T15991 T15990 cc and,Primers
R12342 T15992 T15990 conj probes,Primers
R12343 T15993 T15994 auxpass were,provided
R12344 T15994 T15994 ROOT provided,provided
R12345 T15995 T15994 agent by,provided
R12346 T15996 T15995 pobj Assay-on-Demand,by
R12347 T15997 T15999 punct (,Biosystems
R12348 T15998 T15999 compound Applied,Biosystems
R12349 T15999 T15996 parataxis Biosystems,Assay-on-Demand
R12350 T16000 T15999 punct ),Biosystems
R12351 T16001 T15994 punct .,provided
R12352 T16002 T16003 compound PCR,reactions
R12353 T16003 T16005 nsubjpass reactions,carried
R12354 T16004 T16005 auxpass were,carried
R12355 T16005 T16005 ROOT carried,carried
R12356 T16006 T16005 prt out,carried
R12357 T16007 T16005 prep in,carried
R12358 T16008 T16010 det a,volume
R12359 T16009 T16010 amod final,volume
R12360 T16010 T16007 pobj volume,in
R12361 T16011 T16010 prep of,volume
R12362 T16012 T16013 nummod 25,μl
R12363 T16013 T16011 pobj μl,of
R12364 T16014 T16015 punct (,BMP-6
R12365 T16015 T16013 appos BMP-6,μl
R12366 T16016 T16015 punct ),BMP-6
R12367 T16017 T16013 cc or,μl
R12368 T16018 T16019 nummod 20,μl
R12369 T16019 T16013 conj μl,μl
R12370 T16020 T16021 punct (,ID1
R12371 T16021 T16019 appos ID1,μl
R12372 T16022 T16019 punct ),μl
R12373 T16023 T16005 punct .,carried
R12374 T16024 T16025 det The,cDNA
R12375 T16025 T16026 nsubj cDNA,added
R12376 T16026 T16026 ROOT added,added
R12377 T16027 T16026 prep to,added
R12378 T16028 T16029 det each,reaction
R12379 T16029 T16027 pobj reaction,to
R12380 T16030 T16026 ccomp was,added
R12381 T16031 T16030 acomp equivalent,was
R12382 T16032 T16031 prep to,equivalent
R12383 T16033 T16034 det the,input
R12384 T16034 T16032 pobj input,to
R12385 T16035 T16034 prep of,input
R12386 T16036 T16037 nummod 20,ng
R12387 T16037 T16035 pobj ng,of
R12388 T16038 T16037 prep of,ng
R12389 T16039 T16040 amod total,RNA
R12390 T16040 T16038 pobj RNA,of
R12391 T16041 T16026 punct .,added
R12392 T16042 T16044 det The,expression
R12393 T16043 T16044 compound gene,expression
R12394 T16044 T16046 nsubjpass expression,quantified
R12395 T16045 T16046 auxpass was,quantified
R12396 T16046 T16046 ROOT quantified,quantified
R12397 T16047 T16046 advcl using,quantified
R12398 T16048 T16051 det the,method
R12399 T16049 T16051 amod standard,method
R12400 T16050 T16051 compound curve,method
R12401 T16051 T16047 dobj method,using
R12402 T16052 T16053 punct (,BMP6
R12403 T16053 T16051 appos BMP6,method
R12404 T16054 T16053 punct ),BMP6
R12405 T16055 T16051 punct ",",method
R12406 T16056 T16051 cc or,method
R12407 T16057 T16060 det the,method
R12408 T16058 T16060 amod comparative,method
R12409 T16059 T16060 compound CT,method
R12410 T16060 T16051 conj method,method
R12411 T16061 T16062 punct (,Id1
R12412 T16062 T16060 appos Id1,method
R12413 T16063 T16060 punct ),method
R12414 T16064 T16065 mark as,described
R12415 T16065 T16046 advcl described,quantified
R12416 T16066 T16065 prep in,described
R12417 T16067 T16069 compound ABI7700,Bulletin
R12418 T16068 T16069 compound User,Bulletin
R12419 T16069 T16066 pobj Bulletin,in
R12420 T16070 T16069 nummod 2,Bulletin
R12421 T16071 T16073 punct (,Biosystems
R12422 T16072 T16073 compound Applied,Biosystems
R12423 T16073 T16069 appos Biosystems,Bulletin
R12424 T16074 T16073 punct ),Biosystems
R12425 T16075 T16046 punct .,quantified
R12426 T16076 T16077 det The,expression
R12427 T16077 T16078 nsubj expression,was
R12428 T16078 T16078 ROOT was,was
R12429 T16079 T16080 advmod then,normalized
R12430 T16080 T16078 acomp normalized,was
R12431 T16081 T16080 prep to,normalized
R12432 T16082 T16084 det the,level
R12433 T16083 T16084 compound expression,level
R12434 T16084 T16081 pobj level,to
R12435 T16085 T16084 prep of,level
R12436 T16086 T16085 pobj PGK1,of
R12437 T16087 T16078 punct .,was
R12438 T16088 T16090 nsubjpass PGK1,chosen
R12439 T16089 T16090 auxpass was,chosen
R12440 T16090 T16090 ROOT chosen,chosen
R12441 T16091 T16090 punct ",",chosen
R12442 T16092 T16096 mark because,shown
R12443 T16093 T16096 nsubjpass it,shown
R12444 T16094 T16096 aux has,shown
R12445 T16095 T16096 auxpass been,shown
R12446 T16096 T16090 advcl shown,chosen
R12447 T16097 T16098 aux to,have
R12448 T16098 T16096 xcomp have,shown
R12449 T16099 T16101 amod low,variability
R12450 T16100 T16101 compound expression,variability
R12451 T16101 T16098 dobj variability,have
R12452 T16102 T16101 prep among,variability
R12453 T16103 T16104 amod lymphocyte,specimens
R12454 T16104 T16102 pobj specimens,among
R12455 T16105 T16107 nmod [,]
R12456 T16106 T16107 nummod 56,]
R12457 T16107 T16101 appos ],variability
R12458 T16108 T16090 punct .,chosen
R12459 T16109 T16110 compound Expression,levels
R12460 T16110 T16116 nsubjpass levels,related
R12461 T16111 T16110 prep in,levels
R12462 T16112 T16113 compound B,cells
R12463 T16113 T16111 pobj cells,in
R12464 T16114 T16116 auxpass were,related
R12465 T16115 T16116 advmod then,related
R12466 T16116 T16116 ROOT related,related
R12467 T16117 T16116 prep to,related
R12468 T16118 T16120 det the,levels
R12469 T16119 T16120 compound expression,levels
R12470 T16120 T16117 pobj levels,to
R12471 T16121 T16120 prep in,levels
R12472 T16122 T16123 compound Ramos,cells
R12473 T16123 T16121 pobj cells,in
R12474 T16124 T16116 punct .,related
R12633 T16348 T16349 compound Cell,proliferation
R12634 T16349 T16379 nsubjpass proliferation,cultured
R12635 T16350 T16379 prep For,cultured
R12636 T16351 T16350 pobj estimation,For
R12637 T16352 T16351 prep of,estimation
R12638 T16353 T16354 compound DNA,synthesis
R12639 T16354 T16352 pobj synthesis,of
R12640 T16355 T16379 punct ",",cultured
R12641 T16356 T16358 nmod CD19,cells
R12642 T16357 T16358 compound +,cells
R12643 T16358 T16379 nsubjpass cells,cultured
R12644 T16359 T16358 punct (,cells
R12645 T16360 T16361 nummod 7.5,×
R12646 T16361 T16358 appos ×,cells
R12647 T16362 T16364 nummod 104,.2
R12648 T16363 T16364 nummod cells/0,.2
R12649 T16364 T16365 nummod .2,ml
R12650 T16365 T16361 appos ml,×
R12651 T16366 T16361 punct ),×
R12652 T16367 T16361 cc or,×
R12653 T16368 T16369 compound Ramos,cells
R12654 T16369 T16358 appos cells,cells
R12655 T16370 T16371 punct (,1
R12656 T16371 T16374 nummod 1,cells/0
R12657 T16372 T16374 nummod ×,cells/0
R12658 T16373 T16374 nummod 104,cells/0
R12659 T16374 T16377 nmod cells/0,)
R12660 T16375 T16376 nummod .2,ml
R12661 T16376 T16374 appos ml,cells/0
R12662 T16377 T16358 punct ),cells
R12663 T16378 T16379 auxpass were,cultured
R12664 T16379 T16379 ROOT cultured,cultured
R12665 T16380 T16379 prep in,cultured
R12666 T16381 T16380 pobj triplicate,in
R12667 T16382 T16379 prep in,cultured
R12668 T16383 T16384 compound microtiter,wells
R12669 T16384 T16382 pobj wells,in
R12670 T16385 T16379 punct .,cultured
R12671 T16386 T16387 det The,cells
R12672 T16387 T16389 nsubjpass cells,pulsed
R12673 T16388 T16389 auxpass were,pulsed
R12674 T16389 T16389 ROOT pulsed,pulsed
R12675 T16390 T16389 prep with,pulsed
R12676 T16391 T16397 nummod 3.7,thymidine
R12677 T16392 T16397 nummod ×,thymidine
R12678 T16393 T16396 compound 104Bq,]
R12679 T16394 T16396 compound [,]
R12680 T16395 T16396 compound 3H,]
R12681 T16396 T16397 compound ],thymidine
R12682 T16397 T16390 pobj thymidine,with
R12683 T16398 T16399 punct (,Amersham
R12684 T16399 T16397 appos Amersham,thymidine
R12685 T16400 T16399 punct ",",Amersham
R12686 T16401 T16399 conj Buckinghamshire,Amersham
R12687 T16402 T16401 punct ",",Buckinghamshire
R12688 T16403 T16401 conj UK,Buckinghamshire
R12689 T16404 T16389 punct ),pulsed
R12690 T16405 T16389 prep for,pulsed
R12691 T16406 T16409 det the,h
R12692 T16407 T16409 amod last,h
R12693 T16408 T16409 nummod 16,h
R12694 T16409 T16405 pobj h,for
R12695 T16410 T16409 prep of,h
R12696 T16411 T16413 det a,incubation
R12697 T16412 T16413 amod 72-h,incubation
R12698 T16413 T16410 pobj incubation,of
R12699 T16414 T16389 punct .,pulsed
R12700 T16415 T16416 det The,cells
R12701 T16416 T16418 nsubjpass cells,harvested
R12702 T16417 T16418 auxpass were,harvested
R12703 T16418 T16418 ROOT harvested,harvested
R12704 T16419 T16418 advcl using,harvested
R12705 T16420 T16423 det an,harvester
R12706 T16421 T16423 amod automated,harvester
R12707 T16422 T16423 compound cell,harvester
R12708 T16423 T16419 dobj harvester,using
R12709 T16424 T16427 punct (,Company
R12710 T16425 T16427 compound Packard,Company
R12711 T16426 T16427 compound Instrument,Company
R12712 T16427 T16423 appos Company,harvester
R12713 T16428 T16427 punct ",",Company
R12714 T16429 T16427 conj Meriden,Company
R12715 T16430 T16429 punct ",",Meriden
R12716 T16431 T16429 conj CT,Meriden
R12717 T16432 T16431 punct ",",CT
R12718 T16433 T16431 conj USA,CT
R12719 T16434 T16433 punct ),USA
R12720 T16435 T16431 cc and,CT
R12721 T16436 T16440 compound [,incorporation
R12722 T16437 T16438 compound 3H,]
R12723 T16438 T16440 compound ],incorporation
R12724 T16439 T16440 compound thymidine,incorporation
R12725 T16440 T16442 nsubjpass incorporation,determined
R12726 T16441 T16442 auxpass was,determined
R12727 T16442 T16418 parataxis determined,harvested
R12728 T16443 T16442 prep in,determined
R12729 T16444 T16446 det a,counter
R12730 T16445 T16446 compound scintillation,counter
R12731 T16446 T16443 pobj counter,in
R12732 T16447 T16448 punct (,TopCount
R12733 T16448 T16446 appos TopCount,counter
R12734 T16449 T16448 punct ",",TopCount
R12735 T16450 T16453 compound Packard,Inc.
R12736 T16451 T16453 compound Instrument,Inc.
R12737 T16452 T16453 compound Company,Inc.
R12738 T16453 T16448 conj Inc.,TopCount
R12739 T16454 T16453 punct ",",Inc.
R12740 T16455 T16453 npadvmod Meriden,Inc.
R12741 T16456 T16455 punct ",",Meriden
R12742 T16457 T16455 appos CT,Meriden
R12743 T16458 T16455 punct ),Meriden
R12744 T16459 T16442 punct .,determined
R12801 T16540 T16547 nsubjpass Determination,measured
R12802 T16541 T16540 prep of,Determination
R12803 T16542 T16543 compound cell,death
R12804 T16543 T16545 compound death,death
R12805 T16544 T16545 compound Cell,death
R12806 T16545 T16541 pobj death,of
R12807 T16546 T16547 auxpass was,measured
R12808 T16547 T16547 ROOT measured,measured
R12809 T16548 T16547 agent by,measured
R12810 T16549 T16552 amod vital,test
R12811 T16550 T16551 compound dye,exclusion
R12812 T16551 T16552 compound exclusion,test
R12813 T16552 T16548 pobj test,by
R12814 T16553 T16547 prep by,measured
R12815 T16554 T16553 pcomp staining,by
R12816 T16555 T16554 dobj cells,staining
R12817 T16556 T16554 prep with,staining
R12818 T16557 T16558 nummod 5,μg
R12819 T16558 T16562 nmod μg,iodide
R12820 T16559 T16562 nmod /,iodide
R12821 T16560 T16562 nmod ml,iodide
R12822 T16561 T16562 compound propidium,iodide
R12823 T16562 T16556 pobj iodide,with
R12824 T16563 T16562 punct (,iodide
R12825 T16564 T16566 compound [,]
R12826 T16565 T16566 compound PI,]
R12827 T16566 T16562 appos ],iodide
R12828 T16567 T16562 punct ;,iodide
R12829 T16568 T16569 compound Calbiochem,Corp.
R12830 T16569 T16562 conj Corp.,iodide
R12831 T16570 T16569 punct ;,Corp.
R12832 T16571 T16572 compound La,Jolla
R12833 T16572 T16569 npadvmod Jolla,Corp.
R12834 T16573 T16572 punct ",",Jolla
R12835 T16574 T16572 conj CA,Jolla
R12836 T16575 T16572 punct ;,Jolla
R12837 T16576 T16577 nummod 5,mg/ml
R12838 T16577 T16569 appos mg/ml,Corp.
R12839 T16578 T16569 punct ),Corp.
R12840 T16579 T16554 prep for,staining
R12841 T16580 T16581 nummod one,minute
R12842 T16581 T16579 pobj minute,for
R12843 T16582 T16581 prep on,minute
R12844 T16583 T16582 pobj ice,on
R12845 T16584 T16547 punct .,measured
R12846 T16585 T16586 advmod At,least
R12847 T16586 T16587 advmod least,"1,000"
R12848 T16587 T16588 nummod "1,000",cells
R12849 T16588 T16592 nsubjpass cells,run
R12850 T16589 T16588 prep per,cells
R12851 T16590 T16589 pobj sample,per
R12852 T16591 T16592 auxpass were,run
R12853 T16592 T16592 ROOT run,run
R12854 T16593 T16592 prep on,run
R12855 T16594 T16597 det a,flow
R12856 T16595 T16597 compound BD,flow
R12857 T16596 T16597 compound FACSCalibur,flow
R12858 T16597 T16593 pobj flow,on
R12859 T16598 T16592 npadvmod cytometer,run
R12860 T16599 T16592 punct .,run
R12903 T16653 T16654 compound Statistical,analysis
R12904 T16654 T16663 nsubjpass analysis,determined
R12905 T16655 T16657 det The,significance
R12906 T16656 T16657 amod statistical,significance
R12907 T16657 T16663 nsubjpass significance,determined
R12296 T15946 T15947 advmod reverse,transcribed
R12908 T16658 T16657 prep of,significance
R12909 T16659 T16658 pobj differences,of
R12910 T16660 T16659 prep between,differences
R12911 T16661 T16660 pobj groups,between
R12912 T16662 T16663 auxpass was,determined
R12913 T16663 T16663 ROOT determined,determined
R12914 T16664 T16663 advcl using,determined
R12915 T16665 T16670 det the,test
R12916 T16666 T16670 amod paired,test
R12917 T16667 T16670 amod two-tailed,test
R12918 T16668 T16670 nmod Wilcoxon,test
R12919 T16669 T16670 compound nonparametric,test
R12920 T16670 T16664 dobj test,using
R12921 T16671 T16663 punct ",",determined
R12922 T16672 T16663 prep by,determined
R12923 T16673 T16672 pcomp applying,by
R12924 T16674 T16676 nmod SPSS10,software
R12925 T16675 T16676 nummod .1,software
R12926 T16676 T16673 dobj software,applying
R12927 T16677 T16676 punct (,software
R12928 T16678 T16679 compound SPSS,Inc.
R12929 T16679 T16676 appos Inc.,software
R12930 T16680 T16679 punct ",",Inc.
R12931 T16681 T16679 npadvmod Chicago,Inc.
R12932 T16682 T16679 punct ",",Inc.
R12933 T16683 T16679 appos IL,Inc.
R12934 T16684 T16679 punct ",",Inc.
R12935 T16685 T16679 appos USA,Inc.
R12936 T16686 T16679 punct ),Inc.
R12937 T16687 T16663 punct .,determined
R12938 T16688 T16689 nsubj P,values
R12939 T16689 T16689 ROOT values,values
R12940 T16690 T16692 amod less,0.05
R12941 T16691 T16692 quantmod than,0.05
R12942 T16692 T16694 nsubjpass 0.05,considered
R12943 T16693 T16694 auxpass were,considered
R12944 T16694 T16689 ccomp considered,values
R12945 T16695 T16694 oprd significant,considered
R12946 T16696 T16689 punct .,values

UBERON-AE

Id Subject Object Predicate Lexical cue
T13697 168-173 http://purl.obolibrary.org/obo/UBERON_0000178 denotes blood
T13694 112-117 http://purl.obolibrary.org/obo/UBERON_0001977 denotes serum
T13695 812-817 http://purl.obolibrary.org/obo/UBERON_0001977 denotes serum
T13696 899-904 http://purl.obolibrary.org/obo/UBERON_0001977 denotes serum
T13698 413-418 http://purl.obolibrary.org/obo/UBERON_0000178 denotes blood
T14138 1229-1232 http://purl.obolibrary.org/obo/UBERON_0002228 denotes RIB
T14382 1731-1734 http://purl.obolibrary.org/obo/UBERON_0002228 denotes RIB
T15250 3218-3223 http://purl.obolibrary.org/obo/UBERON_0000178 denotes blood
T16605 6594-6600 http://purl.obolibrary.org/obo/UBERON_0002415 denotes tailed

GO-BP

Id Subject Object Predicate Lexical cue
T13706 422-431 http://purl.obolibrary.org/obo/GO_0020013 denotes rosetting
T13707 885-893 http://purl.obolibrary.org/obo/GO_0051923 denotes sulphate
T14148 1063-1069 http://purl.obolibrary.org/obo/GO_0040007 denotes Growth
T14415 1945-1947 http://purl.obolibrary.org/obo/GO_0033968 denotes CA
T14416 2120-2122 http://purl.obolibrary.org/obo/GO_0033968 denotes CA
T14417 2265-2267 http://purl.obolibrary.org/obo/GO_0033968 denotes CA
T14418 2459-2461 http://purl.obolibrary.org/obo/GO_0033968 denotes CA
T14419 2708-2710 http://purl.obolibrary.org/obo/GO_0033968 denotes CA
T14420 2926-2928 http://purl.obolibrary.org/obo/GO_0033968 denotes CA
T14421 2150-2173 http://purl.obolibrary.org/obo/GO_0030513 denotes BMP-signalling pathways
T14422 2150-2173 http://purl.obolibrary.org/obo/GO_0030514 denotes BMP-signalling pathways
T14423 2150-2173 http://purl.obolibrary.org/obo/GO_0030510 denotes BMP-signalling pathways
T14424 2150-2173 http://purl.obolibrary.org/obo/GO_0030509 denotes BMP-signalling pathways
T14425 2154-2164 http://purl.obolibrary.org/obo/GO_0023052 denotes signalling
T14426 2154-2173 http://purl.obolibrary.org/obo/GO_0007165 denotes signalling pathways
T14427 2591-2593 http://purl.obolibrary.org/obo/GO_0004066 denotes AS
T15263 3261-3266 http://purl.obolibrary.org/obo/GO_0019835 denotes lysis
T15264 3354-3362 http://purl.obolibrary.org/obo/GO_0051923 denotes sulphate
T15265 3720-3722 http://purl.obolibrary.org/obo/GO_0003964 denotes RT
T15266 3903-3905 http://purl.obolibrary.org/obo/GO_0003964 denotes RT
T15267 3907-3922 http://purl.obolibrary.org/obo/GO_0003824 denotes Enzyme activity
T15268 4144-4146 http://purl.obolibrary.org/obo/GO_0033968 denotes CA
T15601 4420-4441 http://purl.obolibrary.org/obo/GO_0001171 denotes reverse transcription
T15602 4796-4817 http://purl.obolibrary.org/obo/GO_0001171 denotes Reverse Transcription
T15603 4428-4441 http://purl.obolibrary.org/obo/GO_0006351 denotes transcription
T15604 4804-4817 http://purl.obolibrary.org/obo/GO_0006351 denotes Transcription
T15605 4514-4516 http://purl.obolibrary.org/obo/GO_0003964 denotes RT
T15606 4861-4863 http://purl.obolibrary.org/obo/GO_0033968 denotes CA
T15607 5225-5240 http://purl.obolibrary.org/obo/GO_0010467 denotes gene expression
T16234 5661-5679 http://purl.obolibrary.org/obo/GO_0008283 denotes Cell proliferation
T16235 5698-5711 http://purl.obolibrary.org/obo/GO_0071897 denotes DNA synthesis
T16236 5702-5711 http://purl.obolibrary.org/obo/GO_0009058 denotes synthesis
T16476 6218-6228 http://purl.obolibrary.org/obo/GO_0008219 denotes cell death
T16477 6229-6239 http://purl.obolibrary.org/obo/GO_0008219 denotes Cell death
T16478 6223-6228 http://purl.obolibrary.org/obo/GO_0016265 denotes death
T16479 6234-6239 http://purl.obolibrary.org/obo/GO_0016265 denotes death
T16480 6364-6366 http://purl.obolibrary.org/obo/GO_0033968 denotes CA

GO-MF

Id Subject Object Predicate Lexical cue
T14292 1429-1439 http://purl.obolibrary.org/obo/GO_0003823 denotes antibodies
T14293 1535-1541 http://purl.obolibrary.org/obo/GO_0005488 denotes ligand
T14962 1882-1892 http://purl.obolibrary.org/obo/GO_0003823 denotes antibodies
T14963 2482-2492 http://purl.obolibrary.org/obo/GO_0003823 denotes antibodies
T14964 2760-2770 http://purl.obolibrary.org/obo/GO_0003823 denotes antibodies
T14965 1945-1947 http://purl.obolibrary.org/obo/GO_0033968 denotes CA
T14966 2120-2122 http://purl.obolibrary.org/obo/GO_0033968 denotes CA
T14967 2265-2267 http://purl.obolibrary.org/obo/GO_0033968 denotes CA
T14968 2459-2461 http://purl.obolibrary.org/obo/GO_0033968 denotes CA
T14969 2708-2710 http://purl.obolibrary.org/obo/GO_0033968 denotes CA
T14970 2926-2928 http://purl.obolibrary.org/obo/GO_0033968 denotes CA
T14971 2591-2593 http://purl.obolibrary.org/obo/GO_0004066 denotes AS
T15487 3636-3646 http://purl.obolibrary.org/obo/GO_0003823 denotes antibodies
T15488 3862-3872 http://purl.obolibrary.org/obo/GO_0003823 denotes antibodies
T15489 3720-3722 http://purl.obolibrary.org/obo/GO_0003964 denotes RT
T15490 3903-3905 http://purl.obolibrary.org/obo/GO_0003964 denotes RT
T15491 3907-3922 http://purl.obolibrary.org/obo/GO_0003824 denotes Enzyme activity
T15492 4144-4146 http://purl.obolibrary.org/obo/GO_0033968 denotes CA
T16125 4514-4516 http://purl.obolibrary.org/obo/GO_0003964 denotes RT
T16126 4861-4863 http://purl.obolibrary.org/obo/GO_0033968 denotes CA
T16600 6364-6366 http://purl.obolibrary.org/obo/GO_0033968 denotes CA

GO-CC

Id Subject Object Predicate Lexical cue
T14294 1429-1439 http://purl.obolibrary.org/obo/GO_0019815 denotes antibodies
T14295 1429-1439 http://purl.obolibrary.org/obo/GO_0042571 denotes antibodies
T14972 1882-1892 http://purl.obolibrary.org/obo/GO_0019815 denotes antibodies
T14973 2482-2492 http://purl.obolibrary.org/obo/GO_0019815 denotes antibodies
T14974 2760-2770 http://purl.obolibrary.org/obo/GO_0019815 denotes antibodies
T14975 1882-1892 http://purl.obolibrary.org/obo/GO_0042571 denotes antibodies
T14976 2482-2492 http://purl.obolibrary.org/obo/GO_0042571 denotes antibodies
T14977 2760-2770 http://purl.obolibrary.org/obo/GO_0042571 denotes antibodies
T14978 2780-2784 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T15493 3196-3201 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T15494 3236-3240 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T15495 3636-3646 http://purl.obolibrary.org/obo/GO_0019815 denotes antibodies
T15496 3862-3872 http://purl.obolibrary.org/obo/GO_0019815 denotes antibodies
T15497 3636-3646 http://purl.obolibrary.org/obo/GO_0042571 denotes antibodies
T15498 3862-3872 http://purl.obolibrary.org/obo/GO_0042571 denotes antibodies
T16127 5595-5600 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T16128 5653-5658 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T14109 9-13 http://purl.obolibrary.org/obo/GO_0005623 denotes Cell
T14110 44-49 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T14111 372-377 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T14112 554-559 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T14113 569-574 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T14114 660-664 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T14115 1010-1014 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T14116 734-737 http://purl.obolibrary.org/obo/GO_1990295 denotes PAA
T15201 2937-2941 http://purl.obolibrary.org/obo/GO_0005623 denotes Cell
T15202 2991-2996 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T15203 3029-3034 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T16460 5661-5665 http://purl.obolibrary.org/obo/GO_0005623 denotes Cell
T16461 5719-5724 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T16462 5736-5741 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T16463 5759-5764 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T16464 5774-5779 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T16465 5841-5846 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T16466 5966-5971 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T16467 6006-6010 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T16601 6218-6222 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T16602 6229-6233 http://purl.obolibrary.org/obo/GO_0005623 denotes Cell
T16603 6293-6298 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T16604 6415-6420 http://purl.obolibrary.org/obo/GO_0005623 denotes cells

sentences

Id Subject Object Predicate Lexical cue
T13700 9-21 Sentence denotes Cell culture
T13701 22-156 Sentence denotes If not specified, all cells were cultured in X-VIVO 15™ (BioWhittaker, Verviers, Belgium) serum-free medium at 37°C and 5% CO2 in air.
T13702 157-320 Sentence denotes Peripheral blood was provided by the Blood Bank at Buskerud Regional Hospital with formal agreement by the patients, and approval by the regional ethics committee.
T13703 321-514 Sentence denotes Highly purified resting human B-lymphocytes (CD19+ cells) were isolated from the peripheral blood by rosetting with immunomagnetic beads (Dynabeads M450; Dynal, Oslo, Norway) as described [55].
T13704 515-645 Sentence denotes This procedure yields less than 0.5% T cells, 0.1% NK cells, and 0.5% monocytes as judged by indirect immunofluorescence staining.
T13705 646-1061 Sentence denotes The following cell lines from human lymphoid malignancies were maintained in RPMI 1640 (PAA Laboratories GmbH, Pasching, Austria) supplemented with 10% foetal bovine serum (FCS), 100 units/ml penicillin G, and 100 units/ml of streptomycin sulphate, but serum-starved for at least four hours and cultured in X-VIVO 15™ when included in experiments: EBV-negative BL cell lines Ramos (ECACC 85030802), HL60 (JCRB0085).
T14146 1063-1089 Sentence denotes Growth factors/supplements
T14147 1090-1604 Sentence denotes The following reagents were used at indicated concentrations: recombinant human (rhu) BMP-6 (1 μg/ml, if not specified otherwise), rhu BMP-RIB/ALK-6/Fc Chimera (5 μg/ml), rhu BMPR-II/Fc Chimera (5 μg/ml), and recombinant mouse Noggin (5 μg/ml) were purchased from R&D Systems (Abingdon, UK); Anti-IgM F(ab)2 fragments of rabbit polyclonal antibodies to human IgM heavy chain (37.5 μg/ml) was obtained from Dako, Copenhagen, Denmark and rhu CD40 ligand (CD40L, 10 ng/ml) was a gift from Immunex Corp. (Seattle, WA).
T14407 1606-1674 Sentence denotes Antibodies used for flow cytometric analysis and immunoblot analysis
T14408 1675-1816 Sentence denotes Antibodies against the human BMP-receptors Act-RIA, BMP-RIB, BMPR-II, Act-RIIA and Act-RIIb were purchased from R & D Systems (Abingdon, UK).
T14409 1817-2129 Sentence denotes Detection of the BMP-6-protein has been tried with the following antibodies: goat polyclonal anti-BMP-6 (Santa Cruz, San Diego, CA, USA), monoclonal mouse anti-BMP-6 and polyclonal goat anti-BMP-6 from R & D Systems (Abingdon, UK), and mouse monoclonal anti-BMP-6 (Chemicon International Inc, Temecula, CA, USA).
T14410 2130-2274 Sentence denotes Characterisation of BMP-signalling pathways was done by use of anti-phospho-Smad1, -5, -and 8 polyclonal antibody (Chemicon, Temecula, CA, USA).
T14411 2275-2468 Sentence denotes Expression levels of Id1–3 proteins were detected with polyclonal rabbit antibody and detection was blocked with blocking peptide from Santa Cruz Biotechnology (Santa Cruz, San Diego, CA, USA).
T14412 2469-2640 Sentence denotes As secondary antibodies served anti-mouse, anti-goat or anti-rabbit IgG- horseradish peroxidase (HRP) from Dakocytomation AS (Copenhagen, Denmark) for immunoblot analysis.
T14413 2641-2755 Sentence denotes Anti-β-actin was from Santa-Cruz. From Becton Dickinson (San Jose, CA), we purchased anti-CD19-PE, anti-CD19-FITC.
T14414 2756-2935 Sentence denotes The antibodies used for cell sorting were anti-CD19 PC5 from Immunotech SA (Marseille, France) and anti-CD27 PE from Becton Dickinson, Biosciences Pharmingen (San Diego, CA, USA).
T15100 2937-2949 Sentence denotes Cell sorting
T15101 2950-3170 Sentence denotes Highly purified CD19+CD27- or CD19+CD27+ cells were obtained by staining CD19+ cells with anti-CD27 PE and CD19 PC5 mAbs for 30 minutes at 4°C, followed by washing with PBS and sorting on FACS DiVa from Becton Dickinson.
T15255 3172-3193 Sentence denotes Western-blot analysis
T15256 3194-3380 Sentence denotes B cells from peripheral blood or cultured cell-lines were lysed in lysis buffer (glycerol 10%, β-mercaptoethanol 5%, 0.0625 M Tris-HCL [pH 6.8], sodium dodecyl sulphate [SDS] 2.5%w/vol).
T15257 3381-3574 Sentence denotes Total protein (30–100 μg) from each sample was run on 10% or 12% SDS/polyacrylamide (SDS/PAGE) gels and blotted onto nitrocellulose filters (Protran; Schleicher &Schuell GmbH, Dassel, Germany).
T15258 3575-3724 Sentence denotes Blocking, washing and incubation of the filters with primary antibodies were done according to the manufacturer's protocols at room temperature (RT).
T15259 3725-3906 Sentence denotes After washing with TBS/0.1% Tween-20 (TBS-T), the filters were incubated with horseradish peroxidase (HRP) coupled to relevant secondary antibodies (see above) for 60 minutes at RT.
T15260 3907-4021 Sentence denotes Enzyme activity was visualised by the enhanced chemiluminescence system, ECL+PLUS (Amersham, Buckinghamshire, UK).
T15261 4022-4148 Sentence denotes Densitometric analysis was performed by scanning hyperfilms on a Personal Densitometer SI (Molecular Dynamics, Sunnyvale, CA).
T15262 4149-4312 Sentence denotes Quantification of Id1, Id2 and Id3 protein was calculated by normalizing the specific protein bands to β-actin using Image Quant 5.5 software (Molecular Dynamics).
T15588 4314-4363 Sentence denotes Analysis of BMP-6 messenger RNA (mRNA) expression
T15589 4364-4468 Sentence denotes Endogenous expression of the BMP-6 gene was examined by reverse transcription-polymerase chain reaction.
T15590 4469-4621 Sentence denotes Total RNA was isolated using Absolutely RNA™ RT-PCR Miniprep Kit (Stratagene Europe, Amsterdam, Netherland) according to the manufacturers instructions.
T15591 4622-4723 Sentence denotes Quantification of the isolated total RNA was achieved by using spectrophotometric OD260 measurements.
T15592 4724-4872 Sentence denotes Equal amounts of RNA were then reverse transcribed to cDNA with TaqMan® Reverse Transcription Reagents (Applied Biosystems, Foster City, CA, U.S.A).
T15593 4873-4981 Sentence denotes To measure mRNA expression of BMP6, Id1–Id4 and PGK1 PCR were carried out with TaqMan® universal master mix.
T15594 4982-5055 Sentence denotes Primers and probes were provided by Assay-on-Demand (Applied Biosystems).
T15595 5056-5137 Sentence denotes PCR reactions were carried out in a final volume of 25 μl (BMP-6) or 20 μl (ID1).
T15596 5138-5220 Sentence denotes The cDNA added to each reaction was equivalent to the input of 20 ng of total RNA.
T15597 5221-5392 Sentence denotes The gene expression was quantified using the standard curve method (BMP6), or the comparative CT method (Id1) as described in ABI7700 User Bulletin 2 (Applied Biosystems).
T15598 5393-5460 Sentence denotes The expression was then normalized to the expression level of PGK1.
T15599 5461-5571 Sentence denotes PGK1 was chosen, because it has been shown to have low expression variability among lymphocyte specimens [56].
T15600 5572-5659 Sentence denotes Expression levels in B cells were then related to the expression levels in Ramos cells.
T16230 5661-5679 Sentence denotes Cell proliferation
T16231 5680-5836 Sentence denotes For estimation of DNA synthesis, CD19+ cells (7.5 × 104 cells/0.2 ml) or Ramos cells (1 × 104 cells/0.2 ml) were cultured in triplicate in microtiter wells.
T16232 5837-5961 Sentence denotes The cells were pulsed with 3.7 × 104Bq [3H]thymidine (Amersham, Buckinghamshire, UK) for the last 16 h of a 72-h incubation.
T16233 5962-6199 Sentence denotes The cells were harvested using an automated cell harvester (Packard Instrument Company, Meriden, CT, USA) and [3H]thymidine incorporation was determined in a scintillation counter (TopCount, Packard Instrument Company Inc., Meriden, CT).
T16473 6201-6228 Sentence denotes Determination of cell death
T16474 6229-6399 Sentence denotes Cell death was measured by vital dye exclusion test by staining cells with 5 μg/ml propidium iodide ([PI]; Calbiochem Corp.; La Jolla, CA; 5 mg/ml) for one minute on ice.
T16475 6400-6476 Sentence denotes At least 1,000 cells per sample were run on a BD FACSCalibur flow cytometer.
T16606 6478-6498 Sentence denotes Statistical analysis
T16607 6499-6690 Sentence denotes The statistical significance of differences between groups was determined using the paired two-tailed Wilcoxon nonparametric test, by applying SPSS10.1 software (SPSS Inc., Chicago, IL, USA).
T16608 6691-6743 Sentence denotes P values less than 0.05 were considered significant.
T174 0-7 Sentence denotes Methods
T175 9-21 Sentence denotes Cell culture
T176 22-156 Sentence denotes If not specified, all cells were cultured in X-VIVO 15™ (BioWhittaker, Verviers, Belgium) serum-free medium at 37°C and 5% CO2 in air.
T177 157-320 Sentence denotes Peripheral blood was provided by the Blood Bank at Buskerud Regional Hospital with formal agreement by the patients, and approval by the regional ethics committee.
T178 321-514 Sentence denotes Highly purified resting human B-lymphocytes (CD19+ cells) were isolated from the peripheral blood by rosetting with immunomagnetic beads (Dynabeads M450; Dynal, Oslo, Norway) as described [55].
T179 515-645 Sentence denotes This procedure yields less than 0.5% T cells, 0.1% NK cells, and 0.5% monocytes as judged by indirect immunofluorescence staining.
T180 646-1061 Sentence denotes The following cell lines from human lymphoid malignancies were maintained in RPMI 1640 (PAA Laboratories GmbH, Pasching, Austria) supplemented with 10% foetal bovine serum (FCS), 100 units/ml penicillin G, and 100 units/ml of streptomycin sulphate, but serum-starved for at least four hours and cultured in X-VIVO 15™ when included in experiments: EBV-negative BL cell lines Ramos (ECACC 85030802), HL60 (JCRB0085).
T181 1063-1089 Sentence denotes Growth factors/supplements
T182 1090-1604 Sentence denotes The following reagents were used at indicated concentrations: recombinant human (rhu) BMP-6 (1 μg/ml, if not specified otherwise), rhu BMP-RIB/ALK-6/Fc Chimera (5 μg/ml), rhu BMPR-II/Fc Chimera (5 μg/ml), and recombinant mouse Noggin (5 μg/ml) were purchased from R&D Systems (Abingdon, UK); Anti-IgM F(ab)2 fragments of rabbit polyclonal antibodies to human IgM heavy chain (37.5 μg/ml) was obtained from Dako, Copenhagen, Denmark and rhu CD40 ligand (CD40L, 10 ng/ml) was a gift from Immunex Corp. (Seattle, WA).
T183 1606-1674 Sentence denotes Antibodies used for flow cytometric analysis and immunoblot analysis
T184 1675-1816 Sentence denotes Antibodies against the human BMP-receptors Act-RIA, BMP-RIB, BMPR-II, Act-RIIA and Act-RIIb were purchased from R & D Systems (Abingdon, UK).
T185 1817-2129 Sentence denotes Detection of the BMP-6-protein has been tried with the following antibodies: goat polyclonal anti-BMP-6 (Santa Cruz, San Diego, CA, USA), monoclonal mouse anti-BMP-6 and polyclonal goat anti-BMP-6 from R & D Systems (Abingdon, UK), and mouse monoclonal anti-BMP-6 (Chemicon International Inc, Temecula, CA, USA).
T186 2130-2274 Sentence denotes Characterisation of BMP-signalling pathways was done by use of anti-phospho-Smad1, -5, -and 8 polyclonal antibody (Chemicon, Temecula, CA, USA).
T187 2275-2468 Sentence denotes Expression levels of Id1–3 proteins were detected with polyclonal rabbit antibody and detection was blocked with blocking peptide from Santa Cruz Biotechnology (Santa Cruz, San Diego, CA, USA).
T188 2469-2640 Sentence denotes As secondary antibodies served anti-mouse, anti-goat or anti-rabbit IgG- horseradish peroxidase (HRP) from Dakocytomation AS (Copenhagen, Denmark) for immunoblot analysis.
T189 2641-2674 Sentence denotes Anti-β-actin was from Santa-Cruz.
T190 2675-2755 Sentence denotes From Becton Dickinson (San Jose, CA), we purchased anti-CD19-PE, anti-CD19-FITC.
T191 2756-2935 Sentence denotes The antibodies used for cell sorting were anti-CD19 PC5 from Immunotech SA (Marseille, France) and anti-CD27 PE from Becton Dickinson, Biosciences Pharmingen (San Diego, CA, USA).
T192 2937-2949 Sentence denotes Cell sorting
T193 2950-3170 Sentence denotes Highly purified CD19+CD27- or CD19+CD27+ cells were obtained by staining CD19+ cells with anti-CD27 PE and CD19 PC5 mAbs for 30 minutes at 4°C, followed by washing with PBS and sorting on FACS DiVa from Becton Dickinson.
T194 3172-3193 Sentence denotes Western-blot analysis
T195 3194-3380 Sentence denotes B cells from peripheral blood or cultured cell-lines were lysed in lysis buffer (glycerol 10%, β-mercaptoethanol 5%, 0.0625 M Tris-HCL [pH 6.8], sodium dodecyl sulphate [SDS] 2.5%w/vol).
T196 3381-3574 Sentence denotes Total protein (30–100 μg) from each sample was run on 10% or 12% SDS/polyacrylamide (SDS/PAGE) gels and blotted onto nitrocellulose filters (Protran; Schleicher &Schuell GmbH, Dassel, Germany).
T197 3575-3724 Sentence denotes Blocking, washing and incubation of the filters with primary antibodies were done according to the manufacturer's protocols at room temperature (RT).
T198 3725-3906 Sentence denotes After washing with TBS/0.1% Tween-20 (TBS-T), the filters were incubated with horseradish peroxidase (HRP) coupled to relevant secondary antibodies (see above) for 60 minutes at RT.
T199 3907-4021 Sentence denotes Enzyme activity was visualised by the enhanced chemiluminescence system, ECL+PLUS (Amersham, Buckinghamshire, UK).
T200 4022-4148 Sentence denotes Densitometric analysis was performed by scanning hyperfilms on a Personal Densitometer SI (Molecular Dynamics, Sunnyvale, CA).
T201 4149-4312 Sentence denotes Quantification of Id1, Id2 and Id3 protein was calculated by normalizing the specific protein bands to β-actin using Image Quant 5.5 software (Molecular Dynamics).
T202 4314-4363 Sentence denotes Analysis of BMP-6 messenger RNA (mRNA) expression
T203 4364-4468 Sentence denotes Endogenous expression of the BMP-6 gene was examined by reverse transcription-polymerase chain reaction.
T204 4469-4621 Sentence denotes Total RNA was isolated using Absolutely RNA™ RT-PCR Miniprep Kit (Stratagene Europe, Amsterdam, Netherland) according to the manufacturers instructions.
T205 4622-4723 Sentence denotes Quantification of the isolated total RNA was achieved by using spectrophotometric OD260 measurements.
T206 4724-4872 Sentence denotes Equal amounts of RNA were then reverse transcribed to cDNA with TaqMan® Reverse Transcription Reagents (Applied Biosystems, Foster City, CA, U.S.A).
T207 4873-4981 Sentence denotes To measure mRNA expression of BMP6, Id1–Id4 and PGK1 PCR were carried out with TaqMan® universal master mix.
T208 4982-5055 Sentence denotes Primers and probes were provided by Assay-on-Demand (Applied Biosystems).
T209 5056-5137 Sentence denotes PCR reactions were carried out in a final volume of 25 μl (BMP-6) or 20 μl (ID1).
T210 5138-5220 Sentence denotes The cDNA added to each reaction was equivalent to the input of 20 ng of total RNA.
T211 5221-5392 Sentence denotes The gene expression was quantified using the standard curve method (BMP6), or the comparative CT method (Id1) as described in ABI7700 User Bulletin 2 (Applied Biosystems).
T212 5393-5460 Sentence denotes The expression was then normalized to the expression level of PGK1.
T213 5461-5571 Sentence denotes PGK1 was chosen, because it has been shown to have low expression variability among lymphocyte specimens [56].
T214 5572-5659 Sentence denotes Expression levels in B cells were then related to the expression levels in Ramos cells.
T215 5661-5679 Sentence denotes Cell proliferation
T216 5680-5836 Sentence denotes For estimation of DNA synthesis, CD19+ cells (7.5 × 104 cells/0.2 ml) or Ramos cells (1 × 104 cells/0.2 ml) were cultured in triplicate in microtiter wells.
T217 5837-5961 Sentence denotes The cells were pulsed with 3.7 × 104Bq [3H]thymidine (Amersham, Buckinghamshire, UK) for the last 16 h of a 72-h incubation.
T218 5962-6199 Sentence denotes The cells were harvested using an automated cell harvester (Packard Instrument Company, Meriden, CT, USA) and [3H]thymidine incorporation was determined in a scintillation counter (TopCount, Packard Instrument Company Inc., Meriden, CT).
T219 6201-6228 Sentence denotes Determination of cell death
T220 6229-6399 Sentence denotes Cell death was measured by vital dye exclusion test by staining cells with 5 μg/ml propidium iodide ([PI]; Calbiochem Corp.; La Jolla, CA; 5 mg/ml) for one minute on ice.
T221 6400-6476 Sentence denotes At least 1,000 cells per sample were run on a BD FACSCalibur flow cytometer.
T222 6478-6498 Sentence denotes Statistical analysis
T223 6499-6690 Sentence denotes The statistical significance of differences between groups was determined using the paired two-tailed Wilcoxon nonparametric test, by applying SPSS10.1 software (SPSS Inc., Chicago, IL, USA).
T224 6691-6743 Sentence denotes P values less than 0.05 were considered significant.

events-check-again

Id Subject Object Predicate Lexical cue Speculation
T14121 366-370 Protein denotes CD19
T14320 1176-1181 Protein denotes BMP-6
T14321 1225-1232 Protein denotes BMP-RIB
T14322 1233-1238 Protein denotes ALK-6
T14323 1265-1272 Protein denotes BMPR-II
T14324 1317-1323 Protein denotes Noggin
T14325 1530-1541 Protein denotes CD40 ligand
T14326 1543-1548 Protein denotes CD40L
T15041 1718-1725 Protein denotes Act-RIA
T15042 1727-1734 Protein denotes BMP-RIB
T15043 1736-1743 Protein denotes BMPR-II
T15044 1745-1753 Protein denotes Act-RIIA
T15045 1758-1766 Protein denotes Act-RIIb
T15046 1834-1839 Protein denotes BMP-6
T15047 1915-1920 Protein denotes BMP-6
T15048 1977-1982 Protein denotes BMP-6
T15049 2008-2013 Protein denotes BMP-6
T15050 2075-2080 Protein denotes BMP-6
T15051 2206-2211 Protein denotes Smad1
T15052 2213-2215 Protein denotes -5
T15053 2222-2223 Protein denotes 8
T15054 2275-2292 Gene_expression denotes Expression levels true
T15055 2275-2292 Gene_expression denotes Expression levels true
T15056 2296-2299 Protein denotes Id1
T15057 2300-2301 Protein denotes 3
T15058 2375-2382 Negative_regulation denotes blocked
T15059 2375-2382 Negative_regulation denotes blocked
T15060 2646-2653 Protein denotes β-actin
T15061 2731-2735 Protein denotes CD19
T15062 2745-2749 Protein denotes CD19
T15063 2803-2807 Protein denotes CD19
T15064 2860-2864 Protein denotes CD27
T15226 2966-2970 Protein denotes CD19
T15227 2971-2975 Protein denotes CD27
T15228 2980-2984 Protein denotes CD19
T15229 2985-2989 Protein denotes CD27
T15230 3023-3027 Protein denotes CD19
T15231 3045-3049 Protein denotes CD27
T15232 3057-3061 Protein denotes CD19
T15514 4167-4170 Protein denotes Id1
T15515 4172-4175 Protein denotes Id2
T15516 4180-4183 Protein denotes Id3
T15517 4252-4259 Protein denotes β-actin
T16190 4326-4331 Protein denotes BMP-6
T16191 4353-4363 Transcription denotes expression true
T16192 4353-4363 Transcription denotes expression true
T16193 4353-4363 Transcription denotes expression true
T16194 4353-4363 Transcription denotes expression true
T16195 4375-4385 Gene_expression denotes expression true
T16196 4393-4398 Protein denotes BMP-6
T16197 4884-4899 Transcription denotes mRNA expression true
T16198 4884-4899 Transcription denotes mRNA expression true
T16199 4884-4899 Transcription denotes mRNA expression true
T16200 4884-4899 Transcription denotes mRNA expression true
T16201 4903-4907 Protein denotes BMP6
T16202 4909-4912 Protein denotes Id1
T16203 4913-4916 Protein denotes Id4
T16204 4921-4925 Protein denotes PGK1
T16205 5115-5120 Protein denotes BMP-6
T16206 5230-5240 Gene_expression denotes expression true
T16207 5230-5240 Gene_expression denotes expression true
T16208 5289-5293 Protein denotes BMP6
T16209 5326-5329 Protein denotes Id1
T16210 5435-5445 Gene_expression denotes expression true
T16211 5455-5459 Protein denotes PGK1
T16212 5461-5465 Protein denotes PGK1
T16213 5507-5526 Gene_expression denotes have low expression
T16471 5713-5717 Protein denotes CD19
R11652 T15054 T15058 themeOf Expression levels,blocked
R11653 T15055 T15059 themeOf Expression levels,blocked
R11654 T15056 T15054 themeOf Id1,Expression levels
R11655 T15057 T15055 themeOf 3,Expression levels
R12507 T16196 T16195 themeOf BMP-6,expression
R12508 T16201 T16193 themeOf BMP6,expression
R12509 T16201 T16200 themeOf BMP6,mRNA expression
R12510 T16202 T16194 themeOf Id1,expression
R12511 T16202 T16198 themeOf Id1,mRNA expression
R12512 T16203 T16199 themeOf Id4,mRNA expression
R12513 T16203 T16192 themeOf Id4,expression
R12514 T16204 T16197 themeOf PGK1,mRNA expression
R12515 T16204 T16191 themeOf PGK1,expression
R12516 T16208 T16206 themeOf BMP6,expression
R12517 T16209 T16207 themeOf Id1,expression
R12518 T16211 T16210 themeOf PGK1,expression
R12519 T16212 T16213 themeOf PGK1,have low expression

bionlp-st-ge-2016-reference-tees

Id Subject Object Predicate Lexical cue
T14117 1045-1049 Protein denotes HL60
T14118 1051-1059 Protein denotes JCRB0085
T14296 1221-1228 Protein denotes rhu BMP
T14297 1229-1232 Protein denotes RIB
T14298 1233-1238 Protein denotes ALK-6
T14299 1239-1241 Protein denotes Fc
T14300 1265-1272 Protein denotes BMPR-II
T14301 1273-1275 Protein denotes Fc
T14302 1382-1407 Protein denotes Anti-IgM F(ab)2 fragments
T14303 1443-1464 Protein denotes human IgM heavy chain
T14304 1526-1541 Protein denotes rhu CD40 ligand
T14305 1543-1548 Protein denotes CD40L
T14979 1698-1717 Protein denotes human BMP-receptors
T14980 1736-1743 Protein denotes BMPR-II
T14981 1745-1753 Protein denotes Act-RIIA
T14982 1758-1766 Protein denotes Act-RIIb
T14983 1834-1847 Protein denotes BMP-6-protein
T14984 2275-2285 Gene_expression denotes Expression
T14985 2296-2310 Protein denotes Id1–3 proteins
T14986 2375-2382 Negative_regulation denotes blocked
T14987 2537-2540 Protein denotes IgG
T14988 2542-2564 Protein denotes horseradish peroxidase
T14989 2566-2569 Protein denotes HRP
T14990 2641-2653 Protein denotes Anti-β-actin
T14991 2803-2811 Protein denotes CD19 PC5
T14992 2855-2867 Protein denotes anti-CD27 PE
T15204 2966-2970 Protein denotes CD19
T15205 2971-2975 Protein denotes CD27
T15206 2980-2984 Protein denotes CD19
T15207 2985-2989 Protein denotes CD27
T15208 3023-3028 Protein denotes CD19+
T15209 3040-3052 Protein denotes anti-CD27 PE
T15210 3057-3065 Protein denotes CD19 PC5
T15211 3138-3169 Protein denotes FACS DiVa from Becton Dickinson
T16129 4326-4345 Protein denotes BMP-6 messenger RNA
T16130 4353-4363 Gene_expression denotes expression
T16131 4889-4899 Transcription denotes expression
T16132 4889-4899 Transcription denotes expression
T16133 4889-4899 Transcription denotes expression
T16134 4889-4899 Transcription denotes expression
T16135 4903-4907 Protein denotes BMP6
T16136 4909-4912 Protein denotes Id1
T16137 4913-4916 Protein denotes Id4
T16138 4921-4929 Protein denotes PGK1 PCR
T16139 5435-5445 Gene_expression denotes expression
T16140 5455-5459 Protein denotes PGK1
T16141 5461-5465 Protein denotes PGK1
T16468 5713-5718 Protein denotes CD19+
T15499 3803-3825 Protein denotes horseradish peroxidase
T15500 3827-3830 Protein denotes HRP
T15501 3903-3905 Protein denotes RT
T15502 4167-4170 Protein denotes Id1
T15503 4172-4175 Protein denotes Id2
T15504 4180-4191 Protein denotes Id3 protein
T15505 4252-4259 Protein denotes β-actin
R12477 T16136 T16132 themeOf Id1,expression
R12478 T16137 T16133 themeOf Id4,expression
R12479 T16138 T16134 themeOf PGK1 PCR,expression
R12480 T16140 T16139 themeOf PGK1,expression
R11642 T14984 T14986 themeOf Expression,blocked
R11643 T14985 T14984 themeOf Id1–3 proteins,Expression
R12475 T16129 T16130 themeOf BMP-6 messenger RNA,expression
R12476 T16135 T16131 themeOf BMP6,expression

bionlp-st-ge-2016-reference

Id Subject Object Predicate Lexical cue Speculation
T14402 2646-2653 Protein denotes β-actin
T14403 2731-2735 Protein denotes CD19
T14404 2745-2749 Protein denotes CD19
T14405 2803-2807 Protein denotes CD19
T14406 2860-2864 Protein denotes CD27
T15584 5435-5445 Gene_expression denotes expression true
T15585 5455-5459 Protein denotes PGK1
T15586 5461-5465 Protein denotes PGK1
T15587 5507-5526 Gene_expression denotes have low expression
T15251 4167-4170 Protein denotes Id1
T15252 4172-4175 Protein denotes Id2
T15253 4180-4183 Protein denotes Id3
T15254 4252-4259 Protein denotes β-actin
T14139 1176-1181 Protein denotes BMP-6
T14140 1225-1232 Protein denotes BMP-RIB
T14141 1233-1238 Protein denotes ALK-6
T14142 1265-1272 Protein denotes BMPR-II
T14143 1317-1323 Protein denotes Noggin
T14144 1530-1541 Protein denotes CD40 ligand
T14145 1543-1548 Protein denotes CD40L
T15093 2966-2970 Protein denotes CD19
T15094 2971-2975 Protein denotes CD27
T15095 2980-2984 Protein denotes CD19
T15096 2985-2989 Protein denotes CD27
T15097 3023-3027 Protein denotes CD19
T15098 3045-3049 Protein denotes CD27
T15099 3057-3061 Protein denotes CD19
T13699 366-370 Protein denotes CD19
T15564 4326-4331 Protein denotes BMP-6
T15565 4353-4363 Transcription denotes expression true
T15566 4353-4363 Transcription denotes expression true
T15567 4353-4363 Transcription denotes expression true
T15568 4353-4363 Transcription denotes expression true
T15569 4375-4385 Gene_expression denotes expression true
T15570 4393-4398 Protein denotes BMP-6
T15571 4884-4899 Transcription denotes mRNA expression true
T15572 4884-4899 Transcription denotes mRNA expression true
T15573 4884-4899 Transcription denotes mRNA expression true
T15574 4884-4899 Transcription denotes mRNA expression true
T15575 4903-4907 Protein denotes BMP6
T15576 4909-4912 Protein denotes Id1
T15577 4913-4916 Protein denotes Id4
T15578 4921-4925 Protein denotes PGK1
T15579 5115-5120 Protein denotes BMP-6
T15580 5230-5240 Gene_expression denotes expression true
T15581 5230-5240 Gene_expression denotes expression true
T15582 5289-5293 Protein denotes BMP6
T15583 5326-5329 Protein denotes Id1
T14383 1718-1725 Protein denotes Act-RIA
T14384 1727-1734 Protein denotes BMP-RIB
T14385 1736-1743 Protein denotes BMPR-II
T14386 1745-1753 Protein denotes Act-RIIA
T14387 1758-1766 Protein denotes Act-RIIb
T14388 1834-1839 Protein denotes BMP-6
T14389 1915-1920 Protein denotes BMP-6
T14390 1977-1982 Protein denotes BMP-6
T14391 2008-2013 Protein denotes BMP-6
T14392 2075-2080 Protein denotes BMP-6
T14393 2206-2211 Protein denotes Smad1
T14394 2213-2215 Protein denotes -5
T14395 2222-2223 Protein denotes 8
T14396 2275-2292 Gene_expression denotes Expression levels true
T14397 2275-2292 Gene_expression denotes Expression levels true
T14398 2296-2299 Protein denotes Id1
T14399 2300-2301 Protein denotes 3
T14400 2375-2382 Negative_regulation denotes blocked
T14401 2375-2382 Negative_regulation denotes blocked
T16229 5713-5717 Protein denotes CD19
R11979 T15570 T15569 themeOf BMP-6,expression
R11980 T15575 T15567 themeOf BMP6,expression
R11981 T15575 T15574 themeOf BMP6,mRNA expression
R11982 T15576 T15568 themeOf Id1,expression
R11983 T15576 T15572 themeOf Id1,mRNA expression
R11984 T15577 T15573 themeOf Id4,mRNA expression
R11985 T15577 T15566 themeOf Id4,expression
R11986 T15578 T15571 themeOf PGK1,mRNA expression
R11987 T15578 T15565 themeOf PGK1,expression
R11988 T15582 T15580 themeOf BMP6,expression
R11989 T15583 T15581 themeOf Id1,expression
R11990 T15585 T15584 themeOf PGK1,expression
R11991 T15586 T15587 themeOf PGK1,have low expression
R11160 T14396 T14400 themeOf Expression levels,blocked
R11161 T14397 T14401 themeOf Expression levels,blocked
R11162 T14398 T14396 themeOf Id1,Expression levels
R11163 T14399 T14397 themeOf 3,Expression levels

bionlp-st-ge-2016-uniprot

Id Subject Object Predicate Lexical cue
T13900 366-370 P15391 denotes CD19
T14149 1176-1179 P12643 denotes BMP
T14150 1176-1179 P22004 denotes BMP
T14151 1176-1179 P12644 denotes BMP
T14152 1176-1179 P18075 denotes BMP
T14153 1176-1181 P22004 denotes BMP-6
T14154 1225-1228 P22004 denotes BMP
T14155 1225-1228 P18075 denotes BMP
T14156 1225-1228 P12644 denotes BMP
T14157 1225-1228 P12643 denotes BMP
T14158 1225-1232 O00238 denotes BMP-RIB
T14159 1233-1238 P36898 denotes ALK-6
T14160 1265-1272 Q13873 denotes BMPR-II
T14161 1317-1323 Q13253 denotes Noggin
T14162 1530-1534 P25942 denotes CD40
T14163 1530-1541 P29965 denotes CD40 ligand
T14164 1543-1548 P29965 denotes CD40L
T14661 1704-1707 P12643 denotes BMP
T14662 1704-1707 P12644 denotes BMP
T14663 1704-1707 P18075 denotes BMP
T14664 1704-1707 P22004 denotes BMP
T14665 1718-1725 Q04771 denotes Act-RIA
T14666 1727-1730 P12643 denotes BMP
T14667 1727-1730 P12644 denotes BMP
T14668 1727-1730 P18075 denotes BMP
T14669 1727-1730 P22004 denotes BMP
T14670 1727-1734 O00238 denotes BMP-RIB
T14671 1736-1743 Q13873 denotes BMPR-II
T14672 1745-1753 P27037 denotes Act-RIIA
T14673 1758-1766 Q13705 denotes Act-RIIb
T14674 1834-1837 P12643 denotes BMP
T14675 1834-1837 P12644 denotes BMP
T14676 1834-1837 P22004 denotes BMP
T14677 1834-1837 P18075 denotes BMP
T14678 1834-1839 P22004 denotes BMP-6
T14679 1915-1918 P12643 denotes BMP
T14680 1915-1918 P12644 denotes BMP
T14681 1915-1918 P22004 denotes BMP
T14682 1915-1918 P18075 denotes BMP
T14683 1915-1920 P22004 denotes BMP-6
T14684 1977-1980 P18075 denotes BMP
T14685 1977-1980 P12644 denotes BMP
T14686 1977-1980 P22004 denotes BMP
T14687 1977-1980 P12643 denotes BMP
T14688 1977-1982 P22004 denotes BMP-6
T14689 2008-2011 P18075 denotes BMP
T14690 2008-2011 P12644 denotes BMP
T14691 2008-2011 P22004 denotes BMP
T14692 2008-2011 P12643 denotes BMP
T14693 2008-2013 P22004 denotes BMP-6
T14694 2075-2078 P22004 denotes BMP
T14695 2075-2078 P12644 denotes BMP
T14696 2075-2078 P12643 denotes BMP
T14697 2075-2078 P18075 denotes BMP
T14698 2075-2080 P22004 denotes BMP-6
T14699 2150-2153 P22004 denotes BMP
T14700 2150-2153 P12644 denotes BMP
T14701 2150-2153 P12643 denotes BMP
T14702 2150-2153 P18075 denotes BMP
T14703 2206-2211 Q15797 denotes Smad1
T14704 2296-2299 P41134 denotes Id1
T14705 2646-2653 P60709 denotes β-actin
T14706 2731-2735 P15391 denotes CD19
T14707 2745-2749 P15391 denotes CD19
T14708 2803-2807 P15391 denotes CD19
T14709 2860-2864 P26842 denotes CD27
T15142 2966-2970 P15391 denotes CD19
T15143 2971-2975 P26842 denotes CD27
T15144 2980-2984 P15391 denotes CD19
T15145 2985-2989 P26842 denotes CD27
T15146 3023-3027 P15391 denotes CD19
T15147 3045-3049 P26842 denotes CD27
T15148 3057-3061 P15391 denotes CD19
T15474 3330-3332 P0A7Z4 denotes pH
T15475 4167-4170 P41134 denotes Id1
T15476 4172-4175 Q02363 denotes Id2
T15477 4180-4183 Q02535 denotes Id3
T15478 4252-4259 P60709 denotes β-actin
T15841 4326-4329 P22004 denotes BMP
T15842 4326-4329 P18075 denotes BMP
T15843 4326-4329 P12644 denotes BMP
T15844 4326-4329 P12643 denotes BMP
T15845 4326-4331 P22004 denotes BMP-6
T15846 4393-4396 P18075 denotes BMP
T15847 4393-4396 P12643 denotes BMP
T15848 4393-4396 P12644 denotes BMP
T15849 4393-4396 P22004 denotes BMP
T15850 4393-4398 P22004 denotes BMP-6
T15851 4903-4907 P22004 denotes BMP6
T15852 4909-4912 P41134 denotes Id1
T15853 4913-4916 P47928 denotes Id4
T15854 4921-4925 P00558 denotes PGK1
T15855 5115-5118 P22004 denotes BMP
T15856 5115-5118 P12644 denotes BMP
T15857 5115-5118 P18075 denotes BMP
T15858 5115-5118 P12643 denotes BMP
T15859 5115-5120 P22004 denotes BMP-6
T15860 5132-5135 P41134 denotes ID1
T15861 5289-5293 P22004 denotes BMP6
T15862 5326-5329 P41134 denotes Id1
T15863 5455-5459 P00558 denotes PGK1
T15864 5461-5465 P00558 denotes PGK1
T16343 5713-5717 P15391 denotes CD19

test2

Id Subject Object Predicate Lexical cue Speculation
T13693 366-370 Protein denotes CD19
T14131 1176-1181 Protein denotes BMP-6
T14132 1225-1232 Protein denotes BMP-RIB
T14133 1233-1238 Protein denotes ALK-6
T14134 1265-1272 Protein denotes BMPR-II
T14135 1317-1323 Protein denotes Noggin
T14136 1530-1541 Protein denotes CD40 ligand
T14137 1543-1548 Protein denotes CD40L
T14361 1718-1725 Protein denotes Act-RIA true
T14362 1727-1734 Protein denotes BMP-RIB true
T14363 1736-1743 Protein denotes BMPR-II
T14364 1745-1753 Protein denotes Act-RIIA
T14365 1758-1766 Protein denotes Act-RIIb
T14366 1834-1839 Protein denotes BMP-6
T14367 1915-1920 Protein denotes BMP-6
T14368 1977-1982 Protein denotes BMP-6
T14369 2008-2013 Protein denotes BMP-6
T14370 2075-2080 Protein denotes BMP-6
T14371 2206-2211 Protein denotes Smad1
T14372 2213-2215 Protein denotes -5
T14373 2222-2223 Protein denotes 8
T14374 2275-2285 Gene_expression denotes Expression
T14375 2296-2299 Protein denotes Id1
T14376 2300-2301 Protein denotes 3
T14377 2646-2653 Protein denotes β-actin
T14378 2731-2735 Protein denotes CD19
T14379 2745-2749 Protein denotes CD19
T14380 2803-2807 Protein denotes CD19
T14381 2860-2864 Protein denotes CD27
T15086 2966-2970 Protein denotes CD19
T15087 2971-2975 Protein denotes CD27
T15088 2980-2984 Protein denotes CD19
T15089 2985-2989 Protein denotes CD27
T15090 3023-3027 Protein denotes CD19
T15091 3045-3049 Protein denotes CD27
T15092 3057-3061 Protein denotes CD19
T15245 4149-4163 Negative_regulation denotes Quantification
T15246 4167-4170 Protein denotes Id1
T15247 4172-4175 Protein denotes Id2
T15248 4180-4183 Protein denotes Id3
T15249 4252-4259 Protein denotes β-actin
T15549 4326-4331 Protein denotes BMP-6 true
T15550 4375-4385 Gene_expression denotes expression true
T15551 4393-4398 Protein denotes BMP-6 true
T15552 4884-4899 Transcription denotes mRNA expression true
T15553 4903-4907 Protein denotes BMP6 true
T15554 4909-4912 Protein denotes Id1 true
T15555 4913-4916 Protein denotes Id4 true
T15556 4921-4925 Protein denotes PGK1 true
T15557 5115-5120 Protein denotes BMP-6 true
T15558 5289-5293 Protein denotes BMP6 true
T15559 5326-5329 Protein denotes Id1 true
T15560 5435-5445 Gene_expression denotes expression true
T15561 5455-5459 Protein denotes PGK1
T15562 5461-5465 Protein denotes PGK1
T15563 5572-5582 Gene_expression denotes Expression
T16228 5713-5717 Protein denotes CD19
R11159 T14375 T14374 themeOf Id1,Expression
R11749 T15246 T15245 themeOf Id1,Quantification
R11750 T15247 T15245 themeOf Id2,Quantification
R11751 T15248 T15245 themeOf Id3,Quantification
R11973 T15551 T15550 themeOf BMP-6,expression
R11974 T15553 T15552 themeOf BMP6,mRNA expression
R11975 T15554 T15552 themeOf Id1,mRNA expression
R11976 T15555 T15552 themeOf Id4,mRNA expression
R11977 T15556 T15552 themeOf PGK1,mRNA expression
R11978 T15561 T15560 themeOf PGK1,expression

tees-test

Id Subject Object Predicate Lexical cue
T2567 25-38 32644 denotes not specified
T2568 112-117 UBERON:0001977 denotes serum
T2569 145-148 CHEBI:29325 denotes CO2
T2570 145-148 CHEBI:48828 denotes CO2
T2571 145-148 CHEBI:16526 denotes CO2
T2572 145-148 CHEBI:29334 denotes CO2
T2573 240-246 CHEBI:48341 denotes formal
T2574 345-350 D006801 denotes human
T2575 366-370 PR:P25918 denotes CD19
T2576 366-370 PR:P15391 denotes CD19
T2577 366-370 PR:000001002 denotes CD19
T2578 422-431 GO:0020013 denotes rosetting
T2579 676-681 D006801 denotes human
T2580 691-703 D009369 denotes malignancies
T2581 691-703 D009369 denotes malignancies
T2582 723-727 PR:A5I6L6 denotes RPMI
T2583 723-727 PR:Q6HCV6 denotes RPMI
T2584 723-727 PR:A0KKP7 denotes RPMI
T2585 723-727 PR:P55874 denotes RPMI
T2586 723-727 PR:O88059 denotes RPMI
T2587 723-727 PR:Q5E5I4 denotes RPMI
T2588 723-727 PR:P66283 denotes RPMI
T2589 723-727 PR:P66279 denotes RPMI
T2590 723-727 PR:Q2FXQ0 denotes RPMI
T2591 723-727 PR:P66274 denotes RPMI
T2592 723-727 PR:P0A7Q1 denotes RPMI
T2593 723-727 PR:P66267 denotes RPMI
T2594 723-727 PR:Q32FI2 denotes RPMI
T2595 723-727 PR:P9WH91 denotes RPMI
T2596 723-727 PR:P66280 denotes RPMI
T2597 723-727 PR:Q89WH9 denotes RPMI
T2598 723-727 PR:Q6MU21 denotes RPMI
T2599 723-727 PR:Q817H6 denotes RPMI
T2600 723-727 PR:B7N554 denotes RPMI
T2601 723-727 PR:Q63TM4 denotes RPMI
T2602 723-727 PR:P0A7Q3 denotes RPMI
T2603 723-727 PR:Q8CS76 denotes RPMI
T2604 723-727 PR:Q8UIN8 denotes RPMI
T2605 723-727 PR:P0A163 denotes RPMI
T2606 723-727 PR:Q5F9U2 denotes RPMI
T2607 723-727 PR:Q038A1 denotes RPMI
T2608 723-727 PR:000023841 denotes RPMI
T2609 723-727 PR:B7NT60 denotes RPMI
T2610 723-727 PR:Q9I0A1 denotes RPMI
T2611 723-727 PR:Q62KI4 denotes RPMI
T2612 723-727 PR:Q8F6Q8 denotes RPMI
T2613 723-727 PR:P67917 denotes RPMI
T2614 723-727 PR:Q4ZUG5 denotes RPMI
T2615 734-737 586025 denotes PAA
T2616 734-737 342822 denotes PAA
T2617 734-737 UBERON:0004363 denotes PAA
T2618 734-737 CHEBI:51133 denotes PAA
T2619 734-737 CHEBI:61372 denotes PAA
T2620 734-737 GO:1990295 denotes PAA
T2621 805-811 9913 denotes bovine
T2622 812-817 UBERON:0001977 denotes serum
T2623 838-850 D010400 denotes penicillin G
T2624 838-850 D010400 denotes penicillin G
T2625 838-850 CHEBI:51354 denotes penicillin G
T2626 838-850 CHEBI:18208 denotes penicillin G
T2627 838-850 7980 denotes penicillin G
T2628 872-884 10109 denotes streptomycin
T2629 872-893 D013307 denotes streptomycin sulphate
T2630 872-893 D013307 denotes streptomycin sulphate
T2631 899-904 UBERON:0001977 denotes serum
T2632 994-997 D004854 denotes EBV
T2633 1021-1026 CVCL_0597 denotes Ramos
T2634 1045-1049 CVCL_0002 denotes HL60
T2635 1063-1077 D036341 denotes Growth factors
T2636 1063-1077 D036341 denotes Growth factors
T2637 1104-1112 CHEBI:33893 denotes reagents
T2638 1104-1112 D007202 denotes reagents
T2639 1164-1169 D006801 denotes human
T2640 1176-1181 D055418 denotes BMP-6
T2641 1176-1181 D055418 denotes BMP-6
T2642 1176-1181 Q04906 denotes BMP-6
T2643 1176-1181 PR:000000167 denotes BMP-6
T2644 1176-1181 P20722 denotes BMP-6
T2645 1176-1181 PR:Q04906 denotes BMP-6
T2646 1176-1181 P22004 denotes BMP-6
T2647 1176-1181 PR:P22004 denotes BMP-6
T2648 1176-1181 PR:P20722 denotes BMP-6
T2649 1195-1208 32644 denotes not specified
T2650 1225-1228 CHEBI:60815 denotes BMP
T2651 1225-1228 PR:000000034 denotes BMP
T2652 1225-1228 P35855 denotes BMP
T2653 1229-1232 CHEBI:33942 denotes RIB
T2654 1229-1232 UBERON:0002228 denotes RIB
T2655 1233-1238 PR:000000036 denotes ALK-6
T2656 1233-1238 Q05438 denotes ALK-6
T2657 1233-1238 P36898 denotes ALK-6
T2658 1242-1249 D002678 denotes Chimera
T2659 1242-1249 PR:000003507 denotes Chimera
T2660 1265-1272 Q13873 denotes BMPR-II
T2661 1265-1272 O35607 denotes BMPR-II
T2662 1265-1272 PR:000000037 denotes BMPR-II
T2663 1276-1283 D002678 denotes Chimera
T2664 1276-1283 PR:000003507 denotes Chimera
T2665 1311-1316 10090 denotes mouse
T2666 1311-1316 D051379 denotes mouse
T2667 1317-1323 P97466 denotes Noggin
T2668 1317-1323 Q13253 denotes Noggin
T2669 1317-1323 Q9YHT8 denotes Noggin
T2670 1317-1323 PR:000000021 denotes Noggin
T2671 1317-1323 O93525 denotes Noggin
T2672 1317-1323 P49011 denotes Noggin
T2673 1317-1323 Q62809 denotes Noggin
T2674 1382-1390 C044203 denotes Anti-IgM
T2675 1393-1397 CVCL_LJ79 denotes ab)2
T2676 1411-1417 D011817 denotes rabbit
T2677 1411-1417 9986 denotes rabbit
T2678 1411-1417 813859 denotes rabbit
T2679 1429-1439 D000906 denotes antibodies
T2680 1440-1448 PR:P48775 denotes to human
T2681 1443-1448 D006801 denotes human
T2682 1449-1452 D007075 denotes IgM
T2683 1453-1464 D006362 denotes heavy chain
T2684 1453-1464 D006362 denotes heavy chain
T2685 1459-1464 SO:0000419 denotes chain
T2686 1530-1541 D023201 denotes CD40 ligand
T2687 1530-1541 D023201 denotes CD40 ligand
T2688 1530-1541 P51749 denotes CD40 ligand
T2689 1530-1541 Q9BDM7 denotes CD40 ligand
T2690 1530-1541 Q9BDN3 denotes CD40 ligand
T2691 1530-1541 Q9I8D8 denotes CD40 ligand
T2692 1530-1541 P29965 denotes CD40 ligand
T2693 1530-1541 P63304 denotes CD40 ligand
T2694 1530-1541 O97626 denotes CD40 ligand
T2695 1530-1541 P27548 denotes CD40 ligand
T2696 1530-1541 O97605 denotes CD40 ligand
T2697 1530-1541 Q95MQ5 denotes CD40 ligand
T2698 1530-1541 Q9BDM3 denotes CD40 ligand
T2699 1530-1541 Q9Z2V2 denotes CD40 ligand
T2700 1530-1541 P63305 denotes CD40 ligand
T2701 1535-1541 GO:0005488 denotes ligand
T2702 1543-1548 D023201 denotes CD40L
T2703 1543-1548 D023201 denotes CD40L
T2704 1543-1548 PR:P27548 denotes CD40L
T2705 1543-1548 PR:Q9I8D8 denotes CD40L
T2706 1543-1548 PR:000001950 denotes CD40L
T2707 1543-1548 PR:P29965 denotes CD40L
T2708 1543-1548 PR:Q9Z2V2 denotes CD40L
T2709 1543-1548 P27548 denotes CD40L
T2710 1543-1548 Q95MQ5 denotes CD40L
T2711 1543-1548 Q9BDM3 denotes CD40L
T2712 1543-1548 P63305 denotes CD40L
T2713 1543-1548 Q9BDM7 denotes CD40L
T2714 1543-1548 O97626 denotes CD40L
T2715 1543-1548 O97605 denotes CD40L
T2716 1543-1548 Q9Z2V2 denotes CD40L
T2717 1543-1548 P63304 denotes CD40L
T2718 1543-1548 Q9BDN3 denotes CD40L
T2719 1543-1548 Q9I8D8 denotes CD40L
T2720 1543-1548 P29965 denotes CD40L
T2721 1543-1548 P51749 denotes CD40L
T2722 1547-1552 PR:Q6PL24 denotes L, 10
T2723 1547-1552 PR:000016405 denotes L, 10
T2724 1547-1552 P51417 denotes L, 10
T2725 1547-1552 CVCL_XK53 denotes L, 10
T2726 1606-1616 D000906 denotes Antibodies
T2727 1675-1685 D000906 denotes Antibodies
T2728 1698-1703 D006801 denotes human
T2729 1704-1707 PR:000000034 denotes BMP
T2730 1704-1707 P35855 denotes BMP
T2731 1704-1717 D052004 denotes BMP-receptors
T2732 1727-1730 CHEBI:60815 denotes BMP
T2733 1727-1730 PR:000000034 denotes BMP
T2734 1727-1730 P35855 denotes BMP
T2735 1731-1734 CHEBI:33942 denotes RIB
T2736 1731-1734 UBERON:0002228 denotes RIB
T2737 1736-1743 Q13873 denotes BMPR-II
T2738 1736-1743 O35607 denotes BMPR-II
T2739 1736-1743 PR:000000037 denotes BMPR-II
T2740 1834-1839 D055418 denotes BMP-6
T2741 1834-1839 D055418 denotes BMP-6
T2742 1834-1839 Q04906 denotes BMP-6
T2743 1834-1839 PR:000000167 denotes BMP-6
T2744 1834-1839 P20722 denotes BMP-6
T2745 1834-1839 PR:Q04906 denotes BMP-6
T2746 1834-1839 P22004 denotes BMP-6
T2747 1834-1839 PR:P22004 denotes BMP-6
T2748 1834-1839 PR:P20722 denotes BMP-6
T2749 1840-1847 CHEBI:36080 denotes protein
T2750 1840-1847 CHEBI:11122 denotes protein
T2751 1840-1847 PR:000000001 denotes protein
T2752 1840-1847 GO:0003675 denotes protein
T2753 1840-1847 SO:0000104 denotes protein
T2754 1882-1892 D000906 denotes antibodies
T2755 1894-1898 PR:Q96T53 denotes goat
T2756 1894-1898 PR:B1Q006 denotes goat
T2757 1894-1898 PR:000010226 denotes goat
T2758 1894-1898 PR:B1Q005 denotes goat
T2759 1894-1898 PR:P0C7A3 denotes goat
T2760 1894-1898 9925 denotes goat
T2761 1894-1898 D006041 denotes goat
T2762 1915-1920 D055418 denotes BMP-6
T2763 1915-1920 D055418 denotes BMP-6
T2764 1915-1920 Q04906 denotes BMP-6
T2765 1915-1920 PR:000000167 denotes BMP-6
T2766 1915-1920 P20722 denotes BMP-6
T2767 1915-1920 PR:Q04906 denotes BMP-6
T2768 1915-1920 P22004 denotes BMP-6
T2769 1915-1920 PR:P22004 denotes BMP-6
T2770 1915-1920 PR:P20722 denotes BMP-6
T2771 1922-1927 F1REV3 denotes Santa
T2772 1966-1971 10090 denotes mouse
T2773 1966-1971 D051379 denotes mouse
T2774 1977-1982 D055418 denotes BMP-6
T2775 1977-1982 D055418 denotes BMP-6
T2776 1977-1982 Q04906 denotes BMP-6
T2777 1977-1982 PR:000000167 denotes BMP-6
T2778 1977-1982 P20722 denotes BMP-6
T2779 1977-1982 PR:Q04906 denotes BMP-6
T2780 1977-1982 P22004 denotes BMP-6
T2781 1977-1982 PR:P22004 denotes BMP-6
T2782 1977-1982 PR:P20722 denotes BMP-6
T2783 1998-2002 PR:Q96T53 denotes goat
T2784 1998-2002 PR:B1Q006 denotes goat
T2785 1998-2002 PR:000010226 denotes goat
T2786 1998-2002 PR:B1Q005 denotes goat
T2787 1998-2002 PR:P0C7A3 denotes goat
T2788 1998-2002 9925 denotes goat
T2789 1998-2002 D006041 denotes goat
T2790 2008-2013 D055418 denotes BMP-6
T2791 2008-2013 D055418 denotes BMP-6
T2792 2008-2013 Q04906 denotes BMP-6
T2793 2008-2013 PR:000000167 denotes BMP-6
T2794 2008-2013 P20722 denotes BMP-6
T2795 2008-2013 PR:Q04906 denotes BMP-6
T2796 2008-2013 P22004 denotes BMP-6
T2797 2008-2013 PR:P22004 denotes BMP-6
T2798 2008-2013 PR:P20722 denotes BMP-6
T2799 2053-2058 10090 denotes mouse
T2800 2053-2058 D051379 denotes mouse
T2801 2075-2080 D055418 denotes BMP-6
T2802 2075-2080 D055418 denotes BMP-6
T2803 2075-2080 Q04906 denotes BMP-6
T2804 2075-2080 PR:000000167 denotes BMP-6
T2805 2075-2080 P20722 denotes BMP-6
T2806 2075-2080 PR:Q04906 denotes BMP-6
T2807 2075-2080 P22004 denotes BMP-6
T2808 2075-2080 PR:P22004 denotes BMP-6
T2809 2075-2080 PR:P20722 denotes BMP-6
T2810 2105-2108 UBERON:0035973 denotes Inc
T2811 2150-2153 CHEBI:60815 denotes BMP
T2812 2150-2153 PR:000000034 denotes BMP
T2813 2150-2153 P35855 denotes BMP
T2814 2154-2164 GO:0023052 denotes signalling
T2815 2198-2205 CHEBI:48108 denotes phospho
T2816 2206-2211 PR:Q9I8V2 denotes Smad1
T2817 2206-2211 PR:P97588 denotes Smad1
T2818 2206-2211 Q15797 denotes Smad1
T2819 2206-2211 PR:000000363 denotes Smad1
T2820 2206-2211 PR:P70340 denotes Smad1
T2821 2206-2211 P70340 denotes Smad1
T2822 2206-2211 Q9I962 denotes Smad1
T2823 2206-2211 PR:Q15797 denotes Smad1
T2824 2206-2211 P97588 denotes Smad1
T2825 2206-2211 Q9I8V2 denotes Smad1
T2826 2206-2211 Q1JQA2 denotes Smad1
T2827 2235-2243 GO:0019815 denotes antibody
T2828 2235-2243 GO:0042571 denotes antibody
T2829 2235-2243 GO:0019814 denotes antibody
T2830 2235-2243 GO:0003823 denotes antibody
T2831 2296-2299 PR:P41134 denotes Id1
T2832 2296-2299 PR:P20067 denotes Id1
T2833 2296-2299 PR:P41135 denotes Id1
T2834 2296-2299 PR:000000039 denotes Id1
T2835 2302-2310 CHEBI:36080 denotes proteins
T2836 2341-2347 D011817 denotes rabbit
T2837 2341-2347 9986 denotes rabbit
T2838 2341-2347 813859 denotes rabbit
T2839 2348-2356 GO:0019815 denotes antibody
T2840 2348-2356 GO:0042571 denotes antibody
T2841 2348-2356 GO:0019814 denotes antibody
T2842 2348-2356 GO:0003823 denotes antibody
T2843 2397-2404 PR:000018264 denotes peptide
T2844 2397-2404 PR:000018263 denotes peptide
T2845 2397-2404 CHEBI:16670 denotes peptide
T2846 2397-2404 SO:0001064 denotes peptide
T2847 2410-2415 F1REV3 denotes Santa
T2848 2436-2441 F1REV3 denotes Santa
T2849 2482-2492 D000906 denotes antibodies
T2850 2505-2510 10090 denotes mouse
T2851 2505-2510 D051379 denotes mouse
T2852 2517-2521 9925 denotes goat
T2853 2517-2521 D006041 denotes goat
T2854 2517-2521 PR:Q96T53 denotes goat
T2855 2517-2521 PR:B1Q006 denotes goat
T2856 2517-2521 PR:000010226 denotes goat
T2857 2517-2521 PR:B1Q005 denotes goat
T2858 2517-2521 PR:P0C7A3 denotes goat
T2859 2530-2536 D011817 denotes rabbit
T2860 2530-2536 9986 denotes rabbit
T2861 2530-2536 813859 denotes rabbit
T2862 2537-2564 C051538 denotes IgG- horseradish peroxidase
T2863 2542-2553 3704 denotes horseradish
T2864 2542-2553 D031215 denotes horseradish
T2865 2542-2553 1011043 denotes horseradish
T2866 2542-2569 GO:0004601 denotes horseradish peroxidase (HRP
T2867 2554-2564 P15984 denotes peroxidase
T2868 2554-2564 Q9SCX8 denotes peroxidase
T2869 2554-2564 P85957 denotes peroxidase
T2870 2554-2564 Q01603 denotes peroxidase
T2871 2554-2564 Q55080 denotes peroxidase
T2872 2554-2564 P85317 denotes peroxidase
T2873 2554-2564 Q05855 denotes peroxidase
T2874 2554-2564 A8NK72 denotes peroxidase
T2875 2554-2564 P84714 denotes peroxidase
T2876 2554-2564 P28313 denotes peroxidase
T2877 2554-2564 P28314 denotes peroxidase
T2878 2554-2564 P16147 denotes peroxidase
T2879 2554-2564 B3EWG7 denotes peroxidase
T2880 2554-2564 1600930 denotes peroxidase
T2881 2566-2569 PR:Q0V967 denotes HRP
T2882 2566-2569 Q08168 denotes HRP
T2883 2646-2653 O42161 denotes β-actin
T2884 2646-2653 Q5R1X3 denotes β-actin
T2885 2646-2653 Q9Y702 denotes β-actin
T2886 2646-2653 P60706 denotes β-actin
T2887 2646-2653 P29751 denotes β-actin
T2888 2646-2653 P68143 denotes β-actin
T2889 2646-2653 P83750 denotes β-actin
T2890 2646-2653 P60710 denotes β-actin
T2891 2646-2653 Q71FK5 denotes β-actin
T2892 2646-2653 P60711 denotes β-actin
T2893 2646-2653 Q76N69 denotes β-actin
T2894 2646-2653 P79818 denotes β-actin
T2895 2646-2653 P60713 denotes β-actin
T2896 2646-2653 PR:000003676 denotes β-actin
T2897 2646-2653 Q0PGG4 denotes β-actin
T2898 2646-2653 Q6QAQ1 denotes β-actin
T2899 2646-2653 P84336 denotes β-actin
T2900 2646-2653 O93400 denotes β-actin
T2901 2646-2653 Q711N9 denotes β-actin
T2902 2646-2653 O18840 denotes β-actin
T2903 2646-2653 P48975 denotes β-actin
T2904 2646-2653 Q4L0Y2 denotes β-actin
T2905 2646-2653 Q5R6G0 denotes β-actin
T2906 2646-2653 P83751 denotes β-actin
T2907 2646-2653 P60707 denotes β-actin
T2908 2646-2653 P60709 denotes β-actin
T2909 2646-2653 P60712 denotes β-actin
T2910 2646-2653 Q91ZK5 denotes β-actin
T2911 2646-2653 P15475 denotes β-actin
T2912 2646-2653 Q6NVA9 denotes β-actin
T2913 2646-2653 P84856 denotes β-actin
T2914 2646-2653 Q4R561 denotes β-actin
T2915 2646-2653 P60708 denotes β-actin
T2916 2646-2653 D000199 denotes β-actin
T2917 2663-2668 F1REV3 denotes Santa
T2918 2731-2735 PR:P25918 denotes CD19
T2919 2731-2735 PR:P15391 denotes CD19
T2920 2731-2735 PR:000001002 denotes CD19
T2921 2745-2749 PR:P25918 denotes CD19
T2922 2745-2749 PR:P15391 denotes CD19
T2923 2745-2749 PR:000001002 denotes CD19
T2924 2750-2754 CHEBI:37926 denotes FITC
T2925 2750-2754 D016650 denotes FITC
T2926 2750-2754 D016650 denotes FITC
T2927 2760-2770 D000906 denotes antibodies
T2928 2803-2807 PR:P25918 denotes CD19
T2929 2803-2807 PR:P15391 denotes CD19
T2930 2803-2807 PR:000001002 denotes CD19
T2931 2808-2811 C075359 denotes PC5
T2932 2808-2811 C075359 denotes PC5
T2933 2808-2811 Q92824 denotes PC5
T2934 2808-2811 Q04592 denotes PC5
T2935 2808-2811 PR:000012437 denotes PC5
T2936 2808-2811 P41413 denotes PC5
T2937 2808-2811 PR:Q92824 denotes PC5
T2938 2808-2811 CVCL_M124 denotes PC5
T2939 2860-2864 PR:P41272 denotes CD27
T2940 2860-2864 PR:000001963 denotes CD27
T2941 2860-2864 PR:P26842 denotes CD27
T2942 2966-2970 PR:P25918 denotes CD19
T2943 2966-2970 PR:P15391 denotes CD19
T2944 2966-2970 PR:000001002 denotes CD19
T2945 2971-2975 PR:P41272 denotes CD27
T2946 2971-2975 PR:000001963 denotes CD27
T2947 2971-2975 PR:P26842 denotes CD27
T2948 2980-2984 PR:P25918 denotes CD19
T2949 2980-2984 PR:P15391 denotes CD19
T2950 2980-2984 PR:000001002 denotes CD19
T2951 2985-2989 PR:P41272 denotes CD27
T2952 2985-2989 PR:000001963 denotes CD27
T2953 2985-2989 PR:P26842 denotes CD27
T2954 3023-3027 PR:P25918 denotes CD19
T2955 3023-3027 PR:P15391 denotes CD19
T2956 3023-3027 PR:000001002 denotes CD19
T2957 3045-3049 PR:P41272 denotes CD27
T2958 3045-3049 PR:000001963 denotes CD27
T2959 3045-3049 PR:P26842 denotes CD27
T2960 3057-3061 PR:P25918 denotes CD19
T2961 3057-3061 PR:P15391 denotes CD19
T2962 3057-3061 PR:000001002 denotes CD19
T2963 3062-3065 Q92824 denotes PC5
T2964 3062-3065 Q04592 denotes PC5
T2965 3062-3065 PR:000012437 denotes PC5
T2966 3062-3065 P41413 denotes PC5
T2967 3062-3065 PR:Q92824 denotes PC5
T2968 3062-3065 C075359 denotes PC5
T2969 3062-3065 C075359 denotes PC5
T2970 3062-3065 CVCL_M124 denotes PC5
T2971 3119-3122 D011535 denotes PBS
T2972 3138-3142 P9WQ36 denotes FACS
T2973 3138-3142 P9WQ37 denotes FACS
T2974 3143-3147 PR:Q9Z0F3 denotes DiVa
T2975 3143-3147 PR:P0ABH0 denotes DiVa
T2976 3143-3147 PR:000022714 denotes DiVa
T2977 3143-3147 PR:000004693 denotes DiVa
T2978 3227-3240 CL:0000010 denotes cultured cell
T2979 3261-3266 GO:0019835 denotes lysis
T2980 3267-3273 CHEBI:35225 denotes buffer
T2981 3275-3283 D005990 denotes glycerol
T2982 3275-3283 CHEBI:17754 denotes glycerol
T2983 3275-3283 D005990 denotes glycerol
T2984 3289-3306 CHEBI:41218 denotes β-mercaptoethanol
T2985 3320-3324 CHEBI:9754 denotes Tris
T2986 3320-3324 PR:Q9NQV6 denotes Tris
T2987 3320-3324 PR:000013180 denotes Tris
T2988 3320-3324 PR:Q3UTQ7 denotes Tris
T2989 3325-3328 CHEBI:17883 denotes HCL
T2990 3330-3334 CVCL_UA31 denotes pH 6
T2991 3339-3362 CHEBI:8984 denotes sodium dodecyl sulphate
T2992 3364-3367 CHEBI:8984 denotes SDS
T2993 3364-3367 PR:000014570 denotes SDS
T2994 3364-3367 B5HDJ6 denotes SDS
T2995 3364-3367 PR:000014571 denotes SDS
T2996 3364-3367 PR:P09367 denotes SDS
T2997 3364-3367 PR:P20132 denotes SDS
T2998 3364-3367 PR:Q8R238 denotes SDS
T2999 3364-3367 PR:Q8VBT2 denotes SDS
T3000 3364-3367 PR:Q86B06 denotes SDS
T3001 3364-3367 PR:Q1PFW3 denotes SDS
T3002 3364-3367 D000081003 denotes SDS
T3003 3387-3394 GO:0003675 denotes protein
T3004 3387-3394 CHEBI:36080 denotes protein
T3005 3387-3394 CHEBI:11122 denotes protein
T3006 3387-3394 PR:000000001 denotes protein
T3007 3387-3394 SO:0000104 denotes protein
T3008 3446-3449 CHEBI:8984 denotes SDS
T3009 3446-3449 PR:000014570 denotes SDS
T3010 3446-3449 B5HDJ6 denotes SDS
T3011 3446-3449 PR:000014571 denotes SDS
T3012 3446-3449 PR:P09367 denotes SDS
T3013 3446-3449 PR:P20132 denotes SDS
T3014 3446-3449 PR:Q8R238 denotes SDS
T3015 3446-3449 PR:Q8VBT2 denotes SDS
T3016 3446-3449 PR:Q86B06 denotes SDS
T3017 3446-3449 PR:Q1PFW3 denotes SDS
T3018 3446-3449 D000081003 denotes SDS
T3019 3450-3464 CHEBI:51135 denotes polyacrylamide
T3020 3450-3464 CHEBI:53656 denotes polyacrylamide
T3021 3450-3464 C016679 denotes polyacrylamide
T3022 3450-3464 CHEBI:60766 denotes polyacrylamide
T3023 3466-3469 CHEBI:8984 denotes SDS
T3024 3466-3469 PR:000014570 denotes SDS
T3025 3466-3469 B5HDJ6 denotes SDS
T3026 3466-3469 PR:000014571 denotes SDS
T3027 3466-3469 PR:P09367 denotes SDS
T3028 3466-3469 PR:P20132 denotes SDS
T3029 3466-3469 PR:Q8R238 denotes SDS
T3030 3466-3469 PR:Q8VBT2 denotes SDS
T3031 3466-3469 PR:Q86B06 denotes SDS
T3032 3466-3469 PR:Q1PFW3 denotes SDS
T3033 3466-3469 D000081003 denotes SDS
T3034 3476-3480 D005782 denotes gels
T3035 3476-3480 D005782 denotes gels
T3036 3498-3512 CHEBI:53325 denotes nitrocellulose
T3037 3498-3512 D003101 denotes nitrocellulose
T3038 3498-3512 1425879 denotes nitrocellulose
T3039 3636-3646 D000906 denotes antibodies
T3040 3744-3747 CHEBI:127105 denotes TBS
T3041 3753-3761 CHEBI:9784 denotes Tween-20
T3042 3753-3761 D011136 denotes Tween-20
T3043 3753-3761 D011136 denotes Tween-20
T3044 3753-3761 CHEBI:53424 denotes Tween-20
T3045 3763-3766 CHEBI:127105 denotes TBS
T3046 3803-3814 3704 denotes horseradish
T3047 3803-3814 D031215 denotes horseradish
T3048 3803-3814 1011043 denotes horseradish
T3049 3803-3825 D006735 denotes horseradish peroxidase
T3050 3803-3830 GO:0004601 denotes horseradish peroxidase (HRP
T3051 3815-3825 1600930 denotes peroxidase
T3052 3815-3825 P15984 denotes peroxidase
T3053 3815-3825 Q9SCX8 denotes peroxidase
T3054 3815-3825 P85957 denotes peroxidase
T3055 3815-3825 Q01603 denotes peroxidase
T3056 3815-3825 Q55080 denotes peroxidase
T3057 3815-3825 P85317 denotes peroxidase
T3058 3815-3825 Q05855 denotes peroxidase
T3059 3815-3825 A8NK72 denotes peroxidase
T3060 3815-3825 P84714 denotes peroxidase
T3061 3815-3825 P28313 denotes peroxidase
T3062 3815-3825 P28314 denotes peroxidase
T3063 3815-3825 P16147 denotes peroxidase
T3064 3815-3825 B3EWG7 denotes peroxidase
T3065 3827-3830 PR:Q0V967 denotes HRP
T3066 3827-3830 Q08168 denotes HRP
T3067 3862-3872 D000906 denotes antibodies
T3068 3907-3922 GO:0003824 denotes Enzyme activity
T3069 3980-3983 P55797 denotes ECL
T3070 3980-3983 PR:P55797 denotes ECL
T3071 3980-3983 P83410 denotes ECL
T3072 3980-3983 CL:0000504 denotes ECL
T3073 4167-4170 PR:P41134 denotes Id1
T3074 4167-4170 PR:P20067 denotes Id1
T3075 4167-4170 PR:P41135 denotes Id1
T3076 4167-4170 PR:000000039 denotes Id1
T3077 4172-4175 PR:000000040 denotes Id2
T3078 4172-4175 PR:P41137 denotes Id2
T3079 4172-4175 PR:Q02363 denotes Id2
T3080 4172-4175 PR:P41136 denotes Id2
T3081 4172-4175 C039556 denotes Id2
T3082 4172-4175 C039556 denotes Id2
T3083 4180-4183 PR:P41133 denotes Id3
T3084 4180-4183 PR:P41138 denotes Id3
T3085 4180-4183 PR:Q02535 denotes Id3
T3086 4180-4183 PR:000000041 denotes Id3
T3087 4184-4191 PR:000000001 denotes protein
T3088 4184-4191 CHEBI:36080 denotes protein
T3089 4184-4191 CHEBI:11122 denotes protein
T3090 4184-4191 GO:0003675 denotes protein
T3091 4184-4191 SO:0000104 denotes protein
T3092 4235-4242 PR:000000001 denotes protein
T3093 4235-4242 CHEBI:36080 denotes protein
T3094 4235-4242 CHEBI:11122 denotes protein
T3095 4235-4242 GO:0003675 denotes protein
T3096 4235-4242 SO:0000104 denotes protein
T3097 4252-4259 O42161 denotes β-actin
T3098 4252-4259 Q5R1X3 denotes β-actin
T3099 4252-4259 Q9Y702 denotes β-actin
T3100 4252-4259 P60706 denotes β-actin
T3101 4252-4259 P29751 denotes β-actin
T3102 4252-4259 P68143 denotes β-actin
T3103 4252-4259 P83750 denotes β-actin
T3104 4252-4259 P60710 denotes β-actin
T3105 4252-4259 Q71FK5 denotes β-actin
T3106 4252-4259 P60711 denotes β-actin
T3107 4252-4259 Q76N69 denotes β-actin
T3108 4252-4259 P79818 denotes β-actin
T3109 4252-4259 P60713 denotes β-actin
T3110 4252-4259 PR:000003676 denotes β-actin
T3111 4252-4259 Q0PGG4 denotes β-actin
T3112 4252-4259 Q6QAQ1 denotes β-actin
T3113 4252-4259 P84336 denotes β-actin
T3114 4252-4259 O93400 denotes β-actin
T3115 4252-4259 Q711N9 denotes β-actin
T3116 4252-4259 O18840 denotes β-actin
T3117 4252-4259 P48975 denotes β-actin
T3118 4252-4259 Q4L0Y2 denotes β-actin
T3119 4252-4259 Q5R6G0 denotes β-actin
T3120 4252-4259 P83751 denotes β-actin
T3121 4252-4259 P60707 denotes β-actin
T3122 4252-4259 P60709 denotes β-actin
T3123 4252-4259 P60712 denotes β-actin
T3124 4252-4259 Q91ZK5 denotes β-actin
T3125 4252-4259 P15475 denotes β-actin
T3126 4252-4259 Q6NVA9 denotes β-actin
T3127 4252-4259 P84856 denotes β-actin
T3128 4252-4259 Q4R561 denotes β-actin
T3129 4252-4259 P60708 denotes β-actin
T3130 4252-4259 D000199 denotes β-actin
T3131 4326-4331 D055418 denotes BMP-6
T3132 4326-4331 D055418 denotes BMP-6
T3133 4326-4331 Q04906 denotes BMP-6
T3134 4326-4331 PR:000000167 denotes BMP-6
T3135 4326-4331 P20722 denotes BMP-6
T3136 4326-4331 PR:Q04906 denotes BMP-6
T3137 4326-4331 P22004 denotes BMP-6
T3138 4326-4331 PR:P22004 denotes BMP-6
T3139 4326-4331 PR:P20722 denotes BMP-6
T3140 4332-4345 D012333 denotes messenger RNA
T3141 4332-4345 CHEBI:33699 denotes messenger RNA
T3142 4332-4345 SO:0000234 denotes messenger RNA
T3143 4342-4345 GO:0005562 denotes RNA
T3144 4347-4351 D012333 denotes mRNA
T3145 4347-4351 CHEBI:33699 denotes mRNA
T3146 4347-4351 SO:0000234 denotes mRNA
T3147 4393-4398 D055418 denotes BMP-6
T3148 4393-4398 D055418 denotes BMP-6
T3149 4393-4398 Q04906 denotes BMP-6
T3150 4393-4398 PR:000000167 denotes BMP-6
T3151 4393-4398 P20722 denotes BMP-6
T3152 4393-4398 PR:Q04906 denotes BMP-6
T3153 4393-4398 P22004 denotes BMP-6
T3154 4393-4398 PR:P22004 denotes BMP-6
T3155 4393-4398 PR:P20722 denotes BMP-6
T3156 4399-4403 SO:0000704 denotes gene
T3157 4420-4427 SO:0001031 denotes reverse
T3158 4420-4441 GO:0001171 denotes reverse transcription
T3159 4453-4458 SO:0000419 denotes chain
T3160 4453-4467 MOP:0000635 denotes chain reaction
T3161 4475-4478 CHEBI:33697 denotes RNA
T3162 4475-4478 D012313 denotes RNA
T3163 4475-4478 GO:0005562 denotes RNA
T3164 4475-4478 SO:0000356 denotes RNA
T3165 4509-4512 CHEBI:33697 denotes RNA
T3166 4509-4512 D012313 denotes RNA
T3167 4509-4512 GO:0005562 denotes RNA
T3168 4509-4512 SO:0000356 denotes RNA
T3169 4517-4520 Q41249 denotes PCR
T3170 4517-4520 O66148 denotes PCR
T3171 4517-4520 Q59987 denotes PCR
T3172 4517-4520 Q01289 denotes PCR
T3173 4517-4520 O80333 denotes PCR
T3174 4517-4520 P15904 denotes PCR
T3175 4517-4520 Q9SDT1 denotes PCR
T3176 4517-4520 Q39617 denotes PCR
T3177 4530-4533 PR:P05532 denotes Kit
T3178 4659-4662 CHEBI:33697 denotes RNA
T3179 4659-4662 D012313 denotes RNA
T3180 4659-4662 GO:0005562 denotes RNA
T3181 4659-4662 SO:0000356 denotes RNA
T3182 4741-4744 CHEBI:33697 denotes RNA
T3183 4741-4744 D012313 denotes RNA
T3184 4741-4744 GO:0005562 denotes RNA
T3185 4741-4744 SO:0000356 denotes RNA
T3186 4755-4762 SO:0001031 denotes reverse
T3187 4778-4782 D018076 denotes cDNA
T3188 4778-4782 SO:0000756 denotes cDNA
T3189 4796-4803 SO:0001031 denotes Reverse
T3190 4796-4817 GO:0001171 denotes Reverse Transcription
T3191 4818-4826 CHEBI:33893 denotes Reagents
T3192 4818-4826 D007202 denotes Reagents
T3193 4884-4888 D012333 denotes mRNA
T3194 4884-4888 CHEBI:33699 denotes mRNA
T3195 4884-4888 SO:0000234 denotes mRNA
T3196 4903-4907 D055418 denotes BMP6
T3197 4903-4907 D055418 denotes BMP6
T3198 4903-4907 Q04906 denotes BMP6
T3199 4903-4907 PR:000000167 denotes BMP6
T3200 4903-4907 P20722 denotes BMP6
T3201 4903-4907 PR:Q04906 denotes BMP6
T3202 4903-4907 P22004 denotes BMP6
T3203 4903-4907 PR:P22004 denotes BMP6
T3204 4903-4907 PR:P20722 denotes BMP6
T3205 4909-4912 PR:P41134 denotes Id1
T3206 4909-4912 PR:P20067 denotes Id1
T3207 4909-4912 PR:P41135 denotes Id1
T3208 4909-4912 PR:000000039 denotes Id1
T3209 4913-4916 PR:P47928 denotes Id4
T3210 4913-4916 PR:P41139 denotes Id4
T3211 4913-4916 PR:000000042 denotes Id4
T3212 4921-4924 GO:0004618 denotes PGK
T3213 4921-4925 PR:P00558 denotes PGK1
T3214 4921-4925 PR:P91427 denotes PGK1
T3215 4921-4925 PR:P09411 denotes PGK1
T3216 4921-4925 PR:P00560 denotes PGK1
T3217 4921-4925 PR:O60101 denotes PGK1
T3218 4921-4925 PR:P46273 denotes PGK1
T3219 4921-4925 PR:000012607 denotes PGK1
T3220 4921-4925 PR:Q9LD57 denotes PGK1
T3221 4921-4925 PR:P16617 denotes PGK1
T3222 4926-4929 Q41249 denotes PCR
T3223 4926-4929 O66148 denotes PCR
T3224 4926-4929 Q59987 denotes PCR
T3225 4926-4929 Q01289 denotes PCR
T3226 4926-4929 O80333 denotes PCR
T3227 4926-4929 P15904 denotes PCR
T3228 4926-4929 Q9SDT1 denotes PCR
T3229 4926-4929 Q39617 denotes PCR
T3230 5056-5059 Q41249 denotes PCR
T3231 5056-5059 O66148 denotes PCR
T3232 5056-5059 Q59987 denotes PCR
T3233 5056-5059 Q01289 denotes PCR
T3234 5056-5059 O80333 denotes PCR
T3235 5056-5059 P15904 denotes PCR
T3236 5056-5059 Q9SDT1 denotes PCR
T3237 5056-5059 Q39617 denotes PCR
T3238 5115-5120 Q04906 denotes BMP-6
T3239 5115-5120 PR:000000167 denotes BMP-6
T3240 5115-5120 P20722 denotes BMP-6
T3241 5115-5120 PR:Q04906 denotes BMP-6
T3242 5115-5120 P22004 denotes BMP-6
T3243 5115-5120 PR:P22004 denotes BMP-6
T3244 5115-5120 PR:P20722 denotes BMP-6
T3245 5115-5120 D055418 denotes BMP-6
T3246 5115-5120 D055418 denotes BMP-6
T3247 5132-5135 PR:P41134 denotes ID1
T3248 5132-5135 PR:P20067 denotes ID1
T3249 5132-5135 PR:P41135 denotes ID1
T3250 5132-5135 PR:000000039 denotes ID1
T3251 5142-5146 SO:0000756 denotes cDNA
T3252 5142-5146 D018076 denotes cDNA
T3253 5216-5219 SO:0000356 denotes RNA
T3254 5216-5219 CHEBI:33697 denotes RNA
T3255 5216-5219 D012313 denotes RNA
T3256 5216-5219 GO:0005562 denotes RNA
T3257 5225-5229 SO:0000704 denotes gene
T3258 5225-5240 GO:0010467 denotes gene expression
T3259 5289-5293 D055418 denotes BMP6
T3260 5289-5293 D055418 denotes BMP6
T3261 5289-5293 Q04906 denotes BMP6
T3262 5289-5293 PR:000000167 denotes BMP6
T3263 5289-5293 P20722 denotes BMP6
T3264 5289-5293 PR:Q04906 denotes BMP6
T3265 5289-5293 P22004 denotes BMP6
T3266 5289-5293 PR:P22004 denotes BMP6
T3267 5289-5293 PR:P20722 denotes BMP6
T3268 5326-5329 PR:P41134 denotes Id1
T3269 5326-5329 PR:P20067 denotes Id1
T3270 5326-5329 PR:P41135 denotes Id1
T3271 5326-5329 PR:000000039 denotes Id1
T3272 5455-5458 GO:0004618 denotes PGK
T3273 5455-5459 PR:P00558 denotes PGK1
T3274 5455-5459 PR:P91427 denotes PGK1
T3275 5455-5459 PR:P09411 denotes PGK1
T3276 5455-5459 PR:P00560 denotes PGK1
T3277 5455-5459 PR:O60101 denotes PGK1
T3278 5455-5459 PR:P46273 denotes PGK1
T3279 5455-5459 PR:000012607 denotes PGK1
T3280 5455-5459 PR:Q9LD57 denotes PGK1
T3281 5455-5459 PR:P16617 denotes PGK1
T3282 5461-5464 GO:0004618 denotes PGK
T3283 5461-5465 PR:P00558 denotes PGK1
T3284 5461-5465 PR:P91427 denotes PGK1
T3285 5461-5465 PR:P09411 denotes PGK1
T3286 5461-5465 PR:P00560 denotes PGK1
T3287 5461-5465 PR:O60101 denotes PGK1
T3288 5461-5465 PR:P46273 denotes PGK1
T3289 5461-5465 PR:000012607 denotes PGK1
T3290 5461-5465 PR:Q9LD57 denotes PGK1
T3291 5461-5465 PR:P16617 denotes PGK1
T3292 5545-5555 CL:0000542 denotes lymphocyte
T3293 5647-5652 CVCL_0597 denotes Ramos
T3294 5661-5679 GO:0008283 denotes Cell proliferation
T3295 5666-5679 UBERON:2000098 denotes proliferation
T3296 5698-5701 GO:0005574 denotes DNA
T3297 5698-5701 CHEBI:16991 denotes DNA
T3298 5698-5701 D004247 denotes DNA
T3299 5698-5701 SO:0000352 denotes DNA
T3300 5698-5711 GO:0071897 denotes DNA synthesis
T3301 5713-5717 PR:P25918 denotes CD19
T3302 5713-5717 PR:P15391 denotes CD19
T3303 5713-5717 PR:000001002 denotes CD19
T3304 5753-5758 CVCL_0597 denotes Ramos
T3305 5877-5889 CHEBI:53526 denotes 3H]thymidine
T3306 6073-6085 CHEBI:53526 denotes 3H]thymidine
T3307 6180-6183 UBERON:0035973 denotes Inc
T3308 6218-6228 GO:0008219 denotes cell death
T3309 6229-6239 GO:0008219 denotes Cell death
T3310 6262-6265 PR:Q7ZU29 denotes dye
T3311 6262-6265 PR:P0A9Q1 denotes dye
T3312 6262-6265 PR:000022123 denotes dye
T3313 6262-6265 CHEBI:37958 denotes dye
T3314 6312-6328 D011419 denotes propidium iodide
T3315 6312-6328 D011419 denotes propidium iodide
T3316 6312-6328 CHEBI:51240 denotes propidium iodide
T3317 6364-6369 PR:000004921 denotes CA; 5
T3318 6364-6369 PR:P43165 denotes CA; 5
T3319 6364-6369 PR:P23589 denotes CA; 5
T3320 6364-6369 PR:P35218 denotes CA; 5
T3321 6666-6669 UBERON:0035973 denotes Inc