PMC:1134658 / 10196-11506
Annnotations
pmc-enju-pas
{"project":"pmc-enju-pas","denotations":[{"id":"T6493","span":{"begin":0,"end":5},"obj":"NN"},{"id":"T6494","span":{"begin":6,"end":16},"obj":"NN"},{"id":"T6495","span":{"begin":17,"end":19},"obj":"IN"},{"id":"T6496","span":{"begin":20,"end":21},"obj":"NN"},{"id":"T6497","span":{"begin":22,"end":27},"obj":"NN"},{"id":"T6498","span":{"begin":28,"end":31},"obj":"DT"},{"id":"T6499","span":{"begin":32,"end":36},"obj":"NN"},{"id":"T6500","span":{"begin":37,"end":41},"obj":"IN"},{"id":"T6501","span":{"begin":42,"end":47},"obj":"NN"},{"id":"T6502","span":{"begin":48,"end":51},"obj":"VB"},{"id":"T6503","span":{"begin":52,"end":56},"obj":"VB"},{"id":"T6504","span":{"begin":57,"end":65},"obj":"VB"},{"id":"T6505","span":{"begin":66,"end":68},"obj":"TO"},{"id":"T6506","span":{"begin":69,"end":72},"obj":"VB"},{"id":"T6507","span":{"begin":73,"end":75},"obj":"IN"},{"id":"T6508","span":{"begin":76,"end":78},"obj":"DT"},{"id":"T6509","span":{"begin":79,"end":88},"obj":"JJ"},{"id":"T6510","span":{"begin":89,"end":99},"obj":"NN"},{"id":"T6511","span":{"begin":100,"end":102},"obj":"IN"},{"id":"T6512","span":{"begin":103,"end":115},"obj":"NN"},{"id":"T6513","span":{"begin":116,"end":117},"obj":"-LRB-"},{"id":"T6514","span":{"begin":117,"end":119},"obj":"CD"},{"id":"T6515","span":{"begin":119,"end":120},"obj":"-RRB-"},{"id":"T6516","span":{"begin":121,"end":124},"obj":"CC"},{"id":"T6517","span":{"begin":125,"end":132},"obj":"NN"},{"id":"T6518","span":{"begin":133,"end":134},"obj":"-LRB-"},{"id":"T6519","span":{"begin":134,"end":136},"obj":"CD"},{"id":"T6520","span":{"begin":136,"end":137},"obj":"-RRB-"},{"id":"T6521","span":{"begin":137,"end":138},"obj":"-COMMA-"},{"id":"T6522","span":{"begin":139,"end":147},"obj":"VB"},{"id":"T6523","span":{"begin":148,"end":150},"obj":"PRP"},{"id":"T6524","span":{"begin":151,"end":153},"obj":"TO"},{"id":"T6525","span":{"begin":154,"end":165},"obj":"VB"},{"id":"T6526","span":{"begin":166,"end":173},"obj":"IN"},{"id":"T6527","span":{"begin":174,"end":180},"obj":"JJ"},{"id":"T6528","span":{"begin":181,"end":186},"obj":"JJ"},{"id":"T6529","span":{"begin":187,"end":188},"obj":"NN"},{"id":"T6530","span":{"begin":189,"end":194},"obj":"NN"},{"id":"T6531","span":{"begin":195,"end":200},"obj":"MD"},{"id":"T6532","span":{"begin":201,"end":208},"obj":"VB"},{"id":"T6533","span":{"begin":209,"end":214},"obj":"NN"},{"id":"T6534","span":{"begin":215,"end":219},"obj":"IN"},{"id":"T6535","span":{"begin":220,"end":231},"obj":"NN"},{"id":"T6536","span":{"begin":233,"end":238},"obj":"NN"},{"id":"T6537","span":{"begin":239,"end":244},"obj":"NN"},{"id":"T6538","span":{"begin":244,"end":245},"obj":"-COMMA-"},{"id":"T6539","span":{"begin":246,"end":251},"obj":"WDT"},{"id":"T6540","span":{"begin":252,"end":256},"obj":"VB"},{"id":"T6541","span":{"begin":257,"end":261},"obj":"VB"},{"id":"T6542","span":{"begin":262,"end":271},"obj":"VB"},{"id":"T6543","span":{"begin":272,"end":274},"obj":"TO"},{"id":"T6544","span":{"begin":275,"end":282},"obj":"VB"},{"id":"T6545","span":{"begin":283,"end":288},"obj":"NN"},{"id":"T6546","span":{"begin":289,"end":293},"obj":"NN"},{"id":"T6547","span":{"begin":294,"end":306},"obj":"RB"},{"id":"T6548","span":{"begin":307,"end":308},"obj":"-LRB-"},{"id":"T6549","span":{"begin":308,"end":310},"obj":"CD"},{"id":"T6550","span":{"begin":310,"end":311},"obj":"-RRB-"},{"id":"T6551","span":{"begin":311,"end":312},"obj":"-COMMA-"},{"id":"T6552","span":{"begin":313,"end":316},"obj":"CC"},{"id":"T6553","span":{"begin":317,"end":320},"obj":"DT"},{"id":"T6554","span":{"begin":321,"end":322},"obj":"NN"},{"id":"T6555","span":{"begin":323,"end":327},"obj":"NN"},{"id":"T6556","span":{"begin":328,"end":332},"obj":"NN"},{"id":"T6557","span":{"begin":333,"end":339},"obj":"NN"},{"id":"T6558","span":{"begin":339,"end":340},"obj":"-COMMA-"},{"id":"T6559","span":{"begin":341,"end":347},"obj":"VB"},{"id":"T6560","span":{"begin":348,"end":350},"obj":"IN"},{"id":"T6561","span":{"begin":351,"end":359},"obj":"JJ"},{"id":"T6562","span":{"begin":360,"end":363},"obj":"CC"},{"id":"T6563","span":{"begin":364,"end":372},"obj":"JJ"},{"id":"T6564","span":{"begin":373,"end":381},"obj":"NN"},{"id":"T6565","span":{"begin":381,"end":382},"obj":"-COMMA-"},{"id":"T6566","span":{"begin":383,"end":395},"obj":"RB"},{"id":"T6567","span":{"begin":397,"end":407},"obj":"JJ"},{"id":"T6568","span":{"begin":408,"end":413},"obj":"NN"},{"id":"T6569","span":{"begin":414,"end":418},"obj":"NN"},{"id":"T6570","span":{"begin":419,"end":425},"obj":"NN"},{"id":"T6571","span":{"begin":426,"end":428},"obj":"IN"},{"id":"T6572","span":{"begin":429,"end":435},"obj":"JJ"},{"id":"T6573","span":{"begin":436,"end":437},"obj":"NN"},{"id":"T6574","span":{"begin":438,"end":443},"obj":"NN"},{"id":"T6575","span":{"begin":444,"end":448},"obj":"VB"},{"id":"T6576","span":{"begin":449,"end":459},"obj":"VB"},{"id":"T6577","span":{"begin":460,"end":462},"obj":"IN"},{"id":"T6578","span":{"begin":463,"end":472},"obj":"JJ"},{"id":"T6579","span":{"begin":473,"end":479},"obj":"NN"},{"id":"T6580","span":{"begin":480,"end":485},"obj":"IN"},{"id":"T6581","span":{"begin":486,"end":497},"obj":"NN"},{"id":"T6582","span":{"begin":498,"end":502},"obj":"IN"},{"id":"T6583","span":{"begin":503,"end":511},"obj":"NN"},{"id":"T6584","span":{"begin":512,"end":515},"obj":"IN"},{"id":"T6585","span":{"begin":516,"end":525},"obj":"JJ"},{"id":"T6586","span":{"begin":526,"end":530},"obj":"NN"},{"id":"T6587","span":{"begin":531,"end":537},"obj":"NN"},{"id":"T6588","span":{"begin":539,"end":552},"obj":"RB"},{"id":"T6589","span":{"begin":552,"end":553},"obj":"-COMMA-"},{"id":"T6590","span":{"begin":554,"end":557},"obj":"DT"},{"id":"T6591","span":{"begin":558,"end":571},"obj":"NN"},{"id":"T6592","span":{"begin":572,"end":574},"obj":"IN"},{"id":"T6593","span":{"begin":575,"end":580},"obj":"NN"},{"id":"T6594","span":{"begin":581,"end":585},"obj":"NN"},{"id":"T6595","span":{"begin":586,"end":589},"obj":"VB"},{"id":"T6596","span":{"begin":590,"end":604},"obj":"JJ"},{"id":"T6597","span":{"begin":605,"end":607},"obj":"IN"},{"id":"T6598","span":{"begin":608,"end":610},"obj":"DT"},{"id":"T6599","span":{"begin":611,"end":619},"obj":"JJ"},{"id":"T6600","span":{"begin":620,"end":632},"obj":"JJ"},{"id":"T6601","span":{"begin":633,"end":642},"obj":"JJ"},{"id":"T6602","span":{"begin":643,"end":647},"obj":"NN"},{"id":"T6603","span":{"begin":648,"end":652},"obj":"IN"},{"id":"T6604","span":{"begin":653,"end":660},"obj":"JJ"},{"id":"T6605","span":{"begin":661,"end":673},"obj":"NN"},{"id":"T6606","span":{"begin":674,"end":678},"obj":"CD"},{"id":"T6607","span":{"begin":679,"end":684},"obj":"NN"},{"id":"T6608","span":{"begin":685,"end":690},"obj":"IN"},{"id":"T6609","span":{"begin":691,"end":694},"obj":"DT"},{"id":"T6610","span":{"begin":695,"end":703},"obj":"NN"},{"id":"T6611","span":{"begin":704,"end":706},"obj":"IN"},{"id":"T6612","span":{"begin":707,"end":715},"obj":"NN"},{"id":"T6613","span":{"begin":717,"end":720},"obj":"DT"},{"id":"T6614","span":{"begin":721,"end":726},"obj":"NN"},{"id":"T6615","span":{"begin":727,"end":729},"obj":"IN"},{"id":"T6616","span":{"begin":730,"end":735},"obj":"NN"},{"id":"T6617","span":{"begin":736,"end":740},"obj":"NN"},{"id":"T6618","span":{"begin":741,"end":744},"obj":"VB"},{"id":"T6619","span":{"begin":745,"end":749},"obj":"RB"},{"id":"T6620","span":{"begin":750,"end":752},"obj":"TO"},{"id":"T6621","span":{"begin":753,"end":761},"obj":"NN"},{"id":"T6622","span":{"begin":762,"end":767},"obj":"IN"},{"id":"T6623","span":{"begin":768,"end":770},"obj":"CD"},{"id":"T6624","span":{"begin":771,"end":776},"obj":"NN"},{"id":"T6625","span":{"begin":777,"end":781},"obj":"IN"},{"id":"T6626","span":{"begin":782,"end":793},"obj":"NN"},{"id":"T6627","span":{"begin":794,"end":795},"obj":"-LRB-"},{"id":"T6628","span":{"begin":795,"end":801},"obj":"NN"},{"id":"T6629","span":{"begin":802,"end":804},"obj":"CD"},{"id":"T6630","span":{"begin":804,"end":805},"obj":"-RRB-"},{"id":"T6631","span":{"begin":807,"end":818},"obj":"RB"},{"id":"T6632","span":{"begin":818,"end":819},"obj":"-COMMA-"},{"id":"T6633","span":{"begin":820,"end":824},"obj":"CC"},{"id":"T6634","span":{"begin":825,"end":828},"obj":"NN"},{"id":"T6635","span":{"begin":829,"end":832},"obj":"CC"},{"id":"T6636","span":{"begin":833,"end":838},"obj":"JJ"},{"id":"T6637","span":{"begin":839,"end":847},"obj":"NN"},{"id":"T6638","span":{"begin":848,"end":855},"obj":"VB"},{"id":"T6639","span":{"begin":856,"end":867},"obj":"JJ"},{"id":"T6640","span":{"begin":868,"end":880},"obj":"NN"},{"id":"T6641","span":{"begin":881,"end":883},"obj":"IN"},{"id":"T6642","span":{"begin":884,"end":889},"obj":"NN"},{"id":"T6643","span":{"begin":890,"end":894},"obj":"NN"},{"id":"T6644","span":{"begin":895,"end":896},"obj":"-LRB-"},{"id":"T6645","span":{"begin":896,"end":902},"obj":"NN"},{"id":"T6646","span":{"begin":903,"end":905},"obj":"CD"},{"id":"T6647","span":{"begin":905,"end":906},"obj":"-RRB-"},{"id":"T6648","span":{"begin":908,"end":921},"obj":"RB"},{"id":"T6649","span":{"begin":921,"end":922},"obj":"-COMMA-"},{"id":"T6650","span":{"begin":923,"end":925},"obj":"IN"},{"id":"T6651","span":{"begin":926,"end":927},"obj":"DT"},{"id":"T6652","span":{"begin":928,"end":936},"obj":"JJ"},{"id":"T6653","span":{"begin":937,"end":942},"obj":"NN"},{"id":"T6654","span":{"begin":943,"end":945},"obj":"PRP"},{"id":"T6655","span":{"begin":946,"end":950},"obj":"VB"},{"id":"T6656","span":{"begin":951,"end":956},"obj":"VB"},{"id":"T6657","span":{"begin":957,"end":961},"obj":"IN"},{"id":"T6658","span":{"begin":962,"end":968},"obj":"JJ"},{"id":"T6659","span":{"begin":969,"end":974},"obj":"JJ"},{"id":"T6660","span":{"begin":975,"end":976},"obj":"NN"},{"id":"T6661","span":{"begin":977,"end":982},"obj":"NN"},{"id":"T6662","span":{"begin":983,"end":985},"obj":"VB"},{"id":"T6663","span":{"begin":986,"end":989},"obj":"RB"},{"id":"T6664","span":{"begin":990,"end":997},"obj":"VB"},{"id":"T6665","span":{"begin":998,"end":1003},"obj":"NN"},{"id":"T6666","span":{"begin":1004,"end":1008},"obj":"NN"},{"id":"T6667","span":{"begin":1009,"end":1014},"obj":"IN"},{"id":"T6668","span":{"begin":1015,"end":1025},"obj":"NN"},{"id":"T6669","span":{"begin":1026,"end":1027},"obj":"-LRB-"},{"id":"T6670","span":{"begin":1027,"end":1036},"obj":"NNP"},{"id":"T6671","span":{"begin":1037,"end":1039},"obj":"FW"},{"id":"T6672","span":{"begin":1040,"end":1042},"obj":"FW"},{"id":"T6673","span":{"begin":1042,"end":1043},"obj":"-COMMA-"},{"id":"T6674","span":{"begin":1044,"end":1054},"obj":"NN"},{"id":"T6675","span":{"begin":1055,"end":1057},"obj":"IN"},{"id":"T6676","span":{"begin":1058,"end":1069},"obj":"NN"},{"id":"T6677","span":{"begin":1069,"end":1070},"obj":"-RRB-"},{"id":"T6678","span":{"begin":1072,"end":1076},"obj":"RB"},{"id":"T6679","span":{"begin":1076,"end":1077},"obj":"-COMMA-"},{"id":"T6680","span":{"begin":1078,"end":1080},"obj":"PRP"},{"id":"T6681","span":{"begin":1081,"end":1087},"obj":"VB"},{"id":"T6682","span":{"begin":1088,"end":1090},"obj":"TO"},{"id":"T6683","span":{"begin":1091,"end":1097},"obj":"VB"},{"id":"T6684","span":{"begin":1098,"end":1103},"obj":"NN"},{"id":"T6685","span":{"begin":1104,"end":1111},"obj":"NN"},{"id":"T6686","span":{"begin":1112,"end":1114},"obj":"IN"},{"id":"T6687","span":{"begin":1115,"end":1121},"obj":"JJ"},{"id":"T6688","span":{"begin":1122,"end":1129},"obj":"NN"},{"id":"T6689","span":{"begin":1130,"end":1133},"obj":"CC"},{"id":"T6690","span":{"begin":1134,"end":1140},"obj":"VB"},{"id":"T6691","span":{"begin":1141,"end":1148},"obj":"JJ"},{"id":"T6692","span":{"begin":1149,"end":1161},"obj":"RB"},{"id":"T6693","span":{"begin":1162,"end":1171},"obj":"JJ"},{"id":"T6695","span":{"begin":1184,"end":1191},"obj":"RB"},{"id":"T6696","span":{"begin":1191,"end":1192},"obj":"-COMMA-"},{"id":"T6697","span":{"begin":1193,"end":1195},"obj":"IN"},{"id":"T6698","span":{"begin":1196,"end":1199},"obj":"PRP-DOLLAR-"},{"id":"T6699","span":{"begin":1200,"end":1205},"obj":"NN"},{"id":"T6700","span":{"begin":1206,"end":1211},"obj":"DT"},{"id":"T6701","span":{"begin":1212,"end":1222},"obj":"JJ"},{"id":"T6702","span":{"begin":1223,"end":1233},"obj":"NN"},{"id":"T6703","span":{"begin":1234,"end":1237},"obj":"VB"},{"id":"T6704","span":{"begin":1238,"end":1242},"obj":"RB"},{"id":"T6705","span":{"begin":1243,"end":1252},"obj":"VB"},{"id":"T6706","span":{"begin":1253,"end":1256},"obj":"DT"},{"id":"T6707","span":{"begin":1257,"end":1268},"obj":"JJ"},{"id":"T6708","span":{"begin":1269,"end":1274},"obj":"NN"},{"id":"T6709","span":{"begin":1275,"end":1282},"obj":"NN"},{"id":"T6710","span":{"begin":1283,"end":1286},"obj":"CC"},{"id":"T6711","span":{"begin":1287,"end":1290},"obj":"RB"},{"id":"T6712","span":{"begin":1291,"end":1294},"obj":"DT"},{"id":"T6713","span":{"begin":1295,"end":1301},"obj":"JJ"},{"id":"T6714","span":{"begin":1302,"end":1309},"obj":"NN"},{"id":"T6694","span":{"begin":1172,"end":1182},"obj":"NN"}],"relations":[{"id":"R5081","pred":"arg1Of","subj":"T6494","obj":"T6493"},{"id":"R5082","pred":"arg1Of","subj":"T6494","obj":"T6495"},{"id":"R5083","pred":"arg2Of","subj":"T6497","obj":"T6495"},{"id":"R5084","pred":"arg1Of","subj":"T6497","obj":"T6496"},{"id":"R5085","pred":"arg1Of","subj":"T6499","obj":"T6498"},{"id":"R5086","pred":"arg1Of","subj":"T6501","obj":"T6502"},{"id":"R5087","pred":"arg1Of","subj":"T6501","obj":"T6503"},{"id":"R5088","pred":"arg2Of","subj":"T6501","obj":"T6504"},{"id":"R5089","pred":"arg1Of","subj":"T6501","obj":"T6506"},{"id":"R5090","pred":"arg1Of","subj":"T6501","obj":"T6522"},{"id":"R5091","pred":"arg2Of","subj":"T6504","obj":"T6502"},{"id":"R5092","pred":"arg2Of","subj":"T6504","obj":"T6503"},{"id":"R5093","pred":"arg1Of","subj":"T6504","obj":"T6521"},{"id":"R5094","pred":"arg3Of","subj":"T6506","obj":"T6504"},{"id":"R5095","pred":"arg1Of","subj":"T6506","obj":"T6505"},{"id":"R5096","pred":"arg1Of","subj":"T6506","obj":"T6507"},{"id":"R5097","pred":"arg1Of","subj":"T6510","obj":"T6509"},{"id":"R5098","pred":"arg1Of","subj":"T6510","obj":"T6511"},{"id":"R5099","pred":"arg1Of","subj":"T6510","obj":"T6516"},{"id":"R5100","pred":"arg2Of","subj":"T6512","obj":"T6511"},{"id":"R5101","pred":"arg1Of","subj":"T6512","obj":"T6513"},{"id":"R5102","pred":"arg2Of","subj":"T6514","obj":"T6513"},{"id":"R5103","pred":"arg3Of","subj":"T6515","obj":"T6513"},{"id":"R5104","pred":"arg2Of","subj":"T6516","obj":"T6507"},{"id":"R5105","pred":"arg1Of","subj":"T6516","obj":"T6508"},{"id":"R5106","pred":"arg2Of","subj":"T6517","obj":"T6516"},{"id":"R5107","pred":"arg1Of","subj":"T6517","obj":"T6518"},{"id":"R5108","pred":"arg2Of","subj":"T6519","obj":"T6518"},{"id":"R5109","pred":"arg3Of","subj":"T6520","obj":"T6518"},{"id":"R5110","pred":"arg2Of","subj":"T6521","obj":"T6499"},{"id":"R5111","pred":"arg1Of","subj":"T6521","obj":"T6500"},{"id":"R5112","pred":"arg2Of","subj":"T6522","obj":"T6521"},{"id":"R5113","pred":"arg2Of","subj":"T6523","obj":"T6522"},{"id":"R5114","pred":"arg1Of","subj":"T6523","obj":"T6525"},{"id":"R5115","pred":"arg3Of","subj":"T6525","obj":"T6522"},{"id":"R5116","pred":"arg1Of","subj":"T6525","obj":"T6524"},{"id":"R5117","pred":"arg1Of","subj":"T6530","obj":"T6527"},{"id":"R5118","pred":"arg1Of","subj":"T6530","obj":"T6528"},{"id":"R5119","pred":"arg1Of","subj":"T6530","obj":"T6529"},{"id":"R5120","pred":"arg1Of","subj":"T6530","obj":"T6531"},{"id":"R5121","pred":"arg1Of","subj":"T6530","obj":"T6532"},{"id":"R5122","pred":"arg2Of","subj":"T6532","obj":"T6525"},{"id":"R5123","pred":"arg1Of","subj":"T6532","obj":"T6526"},{"id":"R5124","pred":"arg2Of","subj":"T6532","obj":"T6531"},{"id":"R5125","pred":"arg1Of","subj":"T6532","obj":"T6534"},{"id":"R5126","pred":"arg2Of","subj":"T6533","obj":"T6532"},{"id":"R5127","pred":"arg2Of","subj":"T6535","obj":"T6534"},{"id":"R5128","pred":"arg1Of","subj":"T6537","obj":"T6536"},{"id":"R5129","pred":"arg1Of","subj":"T6537","obj":"T6538"},{"id":"R5130","pred":"arg1Of","subj":"T6537","obj":"T6539"},{"id":"R5131","pred":"arg1Of","subj":"T6537","obj":"T6540"},{"id":"R5132","pred":"arg1Of","subj":"T6537","obj":"T6541"},{"id":"R5133","pred":"arg2Of","subj":"T6537","obj":"T6542"},{"id":"R5134","pred":"arg1Of","subj":"T6537","obj":"T6544"},{"id":"R5135","pred":"arg1Of","subj":"T6537","obj":"T6552"},{"id":"R5136","pred":"arg2Of","subj":"T6542","obj":"T6540"},{"id":"R5137","pred":"arg2Of","subj":"T6542","obj":"T6541"},{"id":"R5138","pred":"arg3Of","subj":"T6544","obj":"T6542"},{"id":"R5139","pred":"arg1Of","subj":"T6544","obj":"T6543"},{"id":"R5140","pred":"arg1Of","subj":"T6544","obj":"T6547"},{"id":"R5141","pred":"arg1Of","subj":"T6544","obj":"T6548"},{"id":"R5142","pred":"arg2Of","subj":"T6546","obj":"T6544"},{"id":"R5143","pred":"arg1Of","subj":"T6546","obj":"T6545"},{"id":"R5144","pred":"arg2Of","subj":"T6549","obj":"T6548"},{"id":"R5145","pred":"arg3Of","subj":"T6550","obj":"T6548"},{"id":"R5146","pred":"arg1Of","subj":"T6552","obj":"T6551"},{"id":"R5147","pred":"arg1Of","subj":"T6552","obj":"T6559"},{"id":"R5148","pred":"arg2Of","subj":"T6557","obj":"T6552"},{"id":"R5149","pred":"arg1Of","subj":"T6557","obj":"T6553"},{"id":"R5150","pred":"arg1Of","subj":"T6557","obj":"T6554"},{"id":"R5151","pred":"arg1Of","subj":"T6557","obj":"T6555"},{"id":"R5152","pred":"arg1Of","subj":"T6557","obj":"T6556"},{"id":"R5153","pred":"arg1Of","subj":"T6559","obj":"T6558"},{"id":"R5154","pred":"arg1Of","subj":"T6559","obj":"T6560"},{"id":"R5155","pred":"arg1Of","subj":"T6559","obj":"T6565"},{"id":"R5156","pred":"arg1Of","subj":"T6559","obj":"T6566"},{"id":"R5157","pred":"arg1Of","subj":"T6561","obj":"T6562"},{"id":"R5158","pred":"arg2Of","subj":"T6563","obj":"T6562"},{"id":"R5159","pred":"arg2Of","subj":"T6564","obj":"T6560"},{"id":"R5160","pred":"arg1Of","subj":"T6564","obj":"T6561"},{"id":"R5161","pred":"arg1Of","subj":"T6564","obj":"T6563"},{"id":"R5162","pred":"arg1Of","subj":"T6570","obj":"T6567"},{"id":"R5163","pred":"arg1Of","subj":"T6570","obj":"T6568"},{"id":"R5164","pred":"arg1Of","subj":"T6570","obj":"T6569"},{"id":"R5165","pred":"arg1Of","subj":"T6570","obj":"T6571"},{"id":"R5166","pred":"arg1Of","subj":"T6570","obj":"T6575"},{"id":"R5167","pred":"arg2Of","subj":"T6570","obj":"T6576"},{"id":"R5168","pred":"arg2Of","subj":"T6574","obj":"T6571"},{"id":"R5169","pred":"arg1Of","subj":"T6574","obj":"T6572"},{"id":"R5170","pred":"arg1Of","subj":"T6574","obj":"T6573"},{"id":"R5171","pred":"arg2Of","subj":"T6576","obj":"T6575"},{"id":"R5172","pred":"arg1Of","subj":"T6576","obj":"T6580"},{"id":"R5173","pred":"arg1Of","subj":"T6579","obj":"T6576"},{"id":"R5174","pred":"arg2Of","subj":"T6579","obj":"T6577"},{"id":"R5175","pred":"arg1Of","subj":"T6579","obj":"T6578"},{"id":"R5176","pred":"arg2Of","subj":"T6581","obj":"T6580"},{"id":"R5177","pred":"arg1Of","subj":"T6581","obj":"T6582"},{"id":"R5178","pred":"arg2Of","subj":"T6583","obj":"T6582"},{"id":"R5179","pred":"arg1Of","subj":"T6583","obj":"T6584"},{"id":"R5180","pred":"arg2Of","subj":"T6587","obj":"T6584"},{"id":"R5181","pred":"arg1Of","subj":"T6587","obj":"T6585"},{"id":"R5182","pred":"arg1Of","subj":"T6587","obj":"T6586"},{"id":"R5183","pred":"arg1Of","subj":"T6591","obj":"T6590"},{"id":"R5184","pred":"arg1Of","subj":"T6591","obj":"T6592"},{"id":"R5185","pred":"arg1Of","subj":"T6591","obj":"T6595"},{"id":"R5186","pred":"arg1Of","subj":"T6591","obj":"T6596"},{"id":"R5187","pred":"arg2Of","subj":"T6594","obj":"T6592"},{"id":"R5188","pred":"arg1Of","subj":"T6594","obj":"T6593"},{"id":"R5189","pred":"arg1Of","subj":"T6595","obj":"T6588"},{"id":"R5190","pred":"arg1Of","subj":"T6595","obj":"T6589"},{"id":"R5191","pred":"arg2Of","subj":"T6596","obj":"T6595"},{"id":"R5192","pred":"arg1Of","subj":"T6596","obj":"T6597"},{"id":"R5193","pred":"arg1Of","subj":"T6601","obj":"T6599"},{"id":"R5194","pred":"arg1Of","subj":"T6601","obj":"T6600"},{"id":"R5195","pred":"arg2Of","subj":"T6602","obj":"T6597"},{"id":"R5196","pred":"arg1Of","subj":"T6602","obj":"T6598"},{"id":"R5197","pred":"arg1Of","subj":"T6602","obj":"T6601"},{"id":"R5198","pred":"arg1Of","subj":"T6602","obj":"T6603"},{"id":"R5199","pred":"arg2Of","subj":"T6607","obj":"T6603"},{"id":"R5200","pred":"arg1Of","subj":"T6607","obj":"T6604"},{"id":"R5201","pred":"arg1Of","subj":"T6607","obj":"T6605"},{"id":"R5202","pred":"arg1Of","subj":"T6607","obj":"T6606"},{"id":"R5203","pred":"arg1Of","subj":"T6607","obj":"T6608"},{"id":"R5204","pred":"arg2Of","subj":"T6610","obj":"T6608"},{"id":"R5205","pred":"arg1Of","subj":"T6610","obj":"T6609"},{"id":"R5206","pred":"arg1Of","subj":"T6610","obj":"T6611"},{"id":"R5207","pred":"arg2Of","subj":"T6612","obj":"T6611"},{"id":"R5208","pred":"arg1Of","subj":"T6614","obj":"T6613"},{"id":"R5209","pred":"arg1Of","subj":"T6614","obj":"T6615"},{"id":"R5210","pred":"arg1Of","subj":"T6614","obj":"T6618"},{"id":"R5211","pred":"arg1Of","subj":"T6614","obj":"T6619"},{"id":"R5212","pred":"arg2Of","subj":"T6617","obj":"T6615"},{"id":"R5213","pred":"arg1Of","subj":"T6617","obj":"T6616"},{"id":"R5214","pred":"arg1Of","subj":"T6618","obj":"T6622"},{"id":"R5215","pred":"arg1Of","subj":"T6618","obj":"T6625"},{"id":"R5216","pred":"arg2Of","subj":"T6619","obj":"T6618"},{"id":"R5217","pred":"arg1Of","subj":"T6619","obj":"T6620"},{"id":"R5218","pred":"arg2Of","subj":"T6621","obj":"T6620"},{"id":"R5219","pred":"arg2Of","subj":"T6624","obj":"T6622"},{"id":"R5220","pred":"arg1Of","subj":"T6624","obj":"T6623"},{"id":"R5221","pred":"arg2Of","subj":"T6626","obj":"T6625"},{"id":"R5222","pred":"arg1Of","subj":"T6626","obj":"T6627"},{"id":"R5223","pred":"arg2Of","subj":"T6628","obj":"T6627"},{"id":"R5224","pred":"arg1Of","subj":"T6628","obj":"T6629"},{"id":"R5225","pred":"arg3Of","subj":"T6630","obj":"T6627"},{"id":"R5226","pred":"arg1Of","subj":"T6634","obj":"T6635"},{"id":"R5227","pred":"arg1Of","subj":"T6635","obj":"T6633"},{"id":"R5228","pred":"arg1Of","subj":"T6635","obj":"T6638"},{"id":"R5229","pred":"arg2Of","subj":"T6637","obj":"T6635"},{"id":"R5230","pred":"arg1Of","subj":"T6637","obj":"T6636"},{"id":"R5231","pred":"arg1Of","subj":"T6638","obj":"T6631"},{"id":"R5232","pred":"arg1Of","subj":"T6638","obj":"T6632"},{"id":"R5233","pred":"arg2Of","subj":"T6640","obj":"T6638"},{"id":"R5234","pred":"arg1Of","subj":"T6640","obj":"T6639"},{"id":"R5235","pred":"arg1Of","subj":"T6640","obj":"T6641"},{"id":"R5236","pred":"arg2Of","subj":"T6643","obj":"T6641"},{"id":"R5237","pred":"arg1Of","subj":"T6643","obj":"T6642"},{"id":"R5238","pred":"arg1Of","subj":"T6643","obj":"T6644"},{"id":"R5239","pred":"arg2Of","subj":"T6645","obj":"T6644"},{"id":"R5240","pred":"arg1Of","subj":"T6645","obj":"T6646"},{"id":"R5241","pred":"arg3Of","subj":"T6647","obj":"T6644"},{"id":"R5242","pred":"arg2Of","subj":"T6653","obj":"T6650"},{"id":"R5243","pred":"arg1Of","subj":"T6653","obj":"T6651"},{"id":"R5244","pred":"arg1Of","subj":"T6653","obj":"T6652"},{"id":"R5245","pred":"arg1Of","subj":"T6654","obj":"T6655"},{"id":"R5246","pred":"arg1Of","subj":"T6654","obj":"T6656"},{"id":"R5247","pred":"arg1Of","subj":"T6656","obj":"T6648"},{"id":"R5248","pred":"arg1Of","subj":"T6656","obj":"T6649"},{"id":"R5249","pred":"arg1Of","subj":"T6656","obj":"T6650"},{"id":"R5250","pred":"arg2Of","subj":"T6656","obj":"T6655"},{"id":"R5251","pred":"arg1Of","subj":"T6661","obj":"T6658"},{"id":"R5252","pred":"arg1Of","subj":"T6661","obj":"T6659"},{"id":"R5253","pred":"arg1Of","subj":"T6661","obj":"T6660"},{"id":"R5254","pred":"arg1Of","subj":"T6661","obj":"T6662"},{"id":"R5255","pred":"arg1Of","subj":"T6661","obj":"T6664"},{"id":"R5256","pred":"arg2Of","subj":"T6664","obj":"T6656"},{"id":"R5257","pred":"arg1Of","subj":"T6664","obj":"T6657"},{"id":"R5258","pred":"arg2Of","subj":"T6664","obj":"T6662"},{"id":"R5259","pred":"arg1Of","subj":"T6664","obj":"T6663"},{"id":"R5260","pred":"arg1Of","subj":"T6664","obj":"T6667"},{"id":"R5261","pred":"arg2Of","subj":"T6666","obj":"T6664"},{"id":"R5262","pred":"arg1Of","subj":"T6666","obj":"T6665"},{"id":"R5263","pred":"arg2Of","subj":"T6668","obj":"T6667"},{"id":"R5264","pred":"arg1Of","subj":"T6668","obj":"T6669"},{"id":"R5265","pred":"arg1Of","subj":"T6672","obj":"T6670"},{"id":"R5266","pred":"arg1Of","subj":"T6672","obj":"T6671"},{"id":"R5267","pred":"arg1Of","subj":"T6672","obj":"T6673"},{"id":"R5268","pred":"arg2Of","subj":"T6673","obj":"T6669"},{"id":"R5269","pred":"arg1Of","subj":"T6673","obj":"T6675"},{"id":"R5270","pred":"arg2Of","subj":"T6674","obj":"T6673"},{"id":"R5271","pred":"arg2Of","subj":"T6676","obj":"T6675"},{"id":"R5272","pred":"arg3Of","subj":"T6677","obj":"T6669"},{"id":"R5273","pred":"arg1Of","subj":"T6680","obj":"T6681"},{"id":"R5274","pred":"arg1Of","subj":"T6680","obj":"T6683"},{"id":"R5275","pred":"arg1Of","subj":"T6681","obj":"T6678"},{"id":"R5276","pred":"arg1Of","subj":"T6681","obj":"T6679"},{"id":"R5277","pred":"arg2Of","subj":"T6683","obj":"T6681"},{"id":"R5278","pred":"arg1Of","subj":"T6683","obj":"T6682"},{"id":"R5279","pred":"arg1Of","subj":"T6683","obj":"T6686"},{"id":"R5280","pred":"arg2Of","subj":"T6685","obj":"T6683"},{"id":"R5281","pred":"arg1Of","subj":"T6685","obj":"T6684"},{"id":"R5282","pred":"arg1Of","subj":"T6688","obj":"T6687"},{"id":"R5283","pred":"arg1Of","subj":"T6688","obj":"T6689"},{"id":"R5284","pred":"arg2Of","subj":"T6689","obj":"T6686"},{"id":"R5285","pred":"arg1Of","subj":"T6693","obj":"T6692"},{"id":"R5286","pred":"arg2Of","subj":"T6694","obj":"T6689"},{"id":"R5287","pred":"arg2Of","subj":"T6694","obj":"T6690"},{"id":"R5288","pred":"arg1Of","subj":"T6694","obj":"T6691"},{"id":"R5289","pred":"arg1Of","subj":"T6694","obj":"T6693"},{"id":"R5290","pred":"arg2Of","subj":"T6699","obj":"T6697"},{"id":"R5291","pred":"arg1Of","subj":"T6699","obj":"T6698"},{"id":"R5292","pred":"arg1Of","subj":"T6702","obj":"T6700"},{"id":"R5293","pred":"arg1Of","subj":"T6702","obj":"T6701"},{"id":"R5294","pred":"arg1Of","subj":"T6702","obj":"T6703"},{"id":"R5295","pred":"arg1Of","subj":"T6702","obj":"T6705"},{"id":"R5296","pred":"arg1Of","subj":"T6705","obj":"T6695"},{"id":"R5297","pred":"arg1Of","subj":"T6705","obj":"T6696"},{"id":"R5298","pred":"arg1Of","subj":"T6705","obj":"T6697"},{"id":"R5299","pred":"arg2Of","subj":"T6705","obj":"T6703"},{"id":"R5300","pred":"arg1Of","subj":"T6705","obj":"T6704"},{"id":"R5301","pred":"arg1Of","subj":"T6709","obj":"T6706"},{"id":"R5302","pred":"arg1Of","subj":"T6709","obj":"T6707"},{"id":"R5303","pred":"arg1Of","subj":"T6709","obj":"T6708"},{"id":"R5304","pred":"arg1Of","subj":"T6709","obj":"T6710"},{"id":"R5305","pred":"arg2Of","subj":"T6710","obj":"T6705"},{"id":"R5306","pred":"arg2Of","subj":"T6714","obj":"T6710"},{"id":"R5307","pred":"arg1Of","subj":"T6714","obj":"T6711"},{"id":"R5308","pred":"arg1Of","subj":"T6714","obj":"T6712"},{"id":"R5309","pred":"arg1Of","subj":"T6714","obj":"T6713"}],"namespaces":[{"prefix":"_base","uri":"http://kmcs.nii.ac.jp/enju/"}],"text":"BMP-6 production in B cells\nThe fact that BMP-6 has been reported to act as an autocrine stimulator in chondrocytes [18] and ovarium [19], prompted us to investigate whether normal human B cells could produce BMP-6 upon stimulation. Ramos cells, which have been described to express BMP-6 mRNA endogenously [20], and the T cell line Jurkat, served as positive and negative controls, respectively. Endogenous BMP-6 mRNA levels in normal B cells were quantified by real-time RT-PCR after stimulation with anti-IgM for different time points. Interestingly, the up-regulation of BMP-6 mRNA was characteristic of an early-to intermediate inducible gene with maximal upregulation four hours after the addition of anti-IgM. The level of BMP-6 mRNA was back to baseline after 24 hours upon stimulation (Figure 10). Furthermore, both FCS and human AB-serum induced significant upregulation of BMP-6 mRNA (Figure 11). Interestingly, in a separate study we have found that normal human T cells do not express BMP-6 mRNA after activation (Sivertsen et al, manuscript in preparation). Next, we wanted to detect BMP-6 protein in normal B-cells and tested various commercially available antibodies. However, in our hands these anti-BMP-6 antibodies did only recognize the recombinant BMP-6 protein and not the native protein."}
bionlp-st-ge-2016-coref
{"project":"bionlp-st-ge-2016-coref","denotations":[{"id":"T6715","span":{"begin":1291,"end":1309},"obj":"Anaphor"},{"id":"T6716","span":{"begin":1269,"end":1274},"obj":"Antecedent"}],"relations":[{"id":"R5310","pred":"boundBy","subj":"T6715","obj":"T6716"}],"namespaces":[{"prefix":"_base","uri":"https://bionlp.dbcls.jp/ontology/ge.owl#"}],"text":"BMP-6 production in B cells\nThe fact that BMP-6 has been reported to act as an autocrine stimulator in chondrocytes [18] and ovarium [19], prompted us to investigate whether normal human B cells could produce BMP-6 upon stimulation. Ramos cells, which have been described to express BMP-6 mRNA endogenously [20], and the T cell line Jurkat, served as positive and negative controls, respectively. Endogenous BMP-6 mRNA levels in normal B cells were quantified by real-time RT-PCR after stimulation with anti-IgM for different time points. Interestingly, the up-regulation of BMP-6 mRNA was characteristic of an early-to intermediate inducible gene with maximal upregulation four hours after the addition of anti-IgM. The level of BMP-6 mRNA was back to baseline after 24 hours upon stimulation (Figure 10). Furthermore, both FCS and human AB-serum induced significant upregulation of BMP-6 mRNA (Figure 11). Interestingly, in a separate study we have found that normal human T cells do not express BMP-6 mRNA after activation (Sivertsen et al, manuscript in preparation). Next, we wanted to detect BMP-6 protein in normal B-cells and tested various commercially available antibodies. However, in our hands these anti-BMP-6 antibodies did only recognize the recombinant BMP-6 protein and not the native protein."}
bionlp-st-ge-2016-spacy-parsed
{"project":"bionlp-st-ge-2016-spacy-parsed","denotations":[{"id":"T6787","span":{"begin":0,"end":5},"obj":"NN"},{"id":"T6788","span":{"begin":6,"end":16},"obj":"NN"},{"id":"T6789","span":{"begin":17,"end":19},"obj":"IN"},{"id":"T6790","span":{"begin":20,"end":21},"obj":"NNP"},{"id":"T6791","span":{"begin":22,"end":27},"obj":"NNS"},{"id":"T6792","span":{"begin":28,"end":31},"obj":"DT"},{"id":"T6793","span":{"begin":32,"end":36},"obj":"NN"},{"id":"T6794","span":{"begin":37,"end":41},"obj":"IN"},{"id":"T6795","span":{"begin":42,"end":47},"obj":"NNP"},{"id":"T6796","span":{"begin":48,"end":51},"obj":"VBZ"},{"id":"T6797","span":{"begin":52,"end":56},"obj":"VBN"},{"id":"T6798","span":{"begin":57,"end":65},"obj":"VBN"},{"id":"T6799","span":{"begin":66,"end":68},"obj":"TO"},{"id":"T6800","span":{"begin":69,"end":72},"obj":"VB"},{"id":"T6801","span":{"begin":73,"end":75},"obj":"IN"},{"id":"T6802","span":{"begin":76,"end":78},"obj":"DT"},{"id":"T6803","span":{"begin":79,"end":88},"obj":"NN"},{"id":"T6804","span":{"begin":89,"end":99},"obj":"NN"},{"id":"T6805","span":{"begin":100,"end":102},"obj":"IN"},{"id":"T6806","span":{"begin":103,"end":115},"obj":"NNS"},{"id":"T6807","span":{"begin":116,"end":117},"obj":"NNP"},{"id":"T6808","span":{"begin":117,"end":119},"obj":"CD"},{"id":"T6809","span":{"begin":119,"end":120},"obj":"NNP"},{"id":"T6810","span":{"begin":121,"end":124},"obj":"CC"},{"id":"T6811","span":{"begin":125,"end":132},"obj":"NN"},{"id":"T6812","span":{"begin":133,"end":134},"obj":"NNP"},{"id":"T6813","span":{"begin":134,"end":136},"obj":"CD"},{"id":"T6814","span":{"begin":136,"end":137},"obj":"NNP"},{"id":"T6815","span":{"begin":137,"end":138},"obj":","},{"id":"T6816","span":{"begin":139,"end":147},"obj":"VBD"},{"id":"T6817","span":{"begin":148,"end":150},"obj":"PRP"},{"id":"T6818","span":{"begin":151,"end":153},"obj":"TO"},{"id":"T6819","span":{"begin":154,"end":165},"obj":"VB"},{"id":"T6820","span":{"begin":166,"end":173},"obj":"IN"},{"id":"T6821","span":{"begin":174,"end":180},"obj":"JJ"},{"id":"T6822","span":{"begin":181,"end":186},"obj":"JJ"},{"id":"T6823","span":{"begin":187,"end":188},"obj":"NNP"},{"id":"T6824","span":{"begin":189,"end":194},"obj":"NNS"},{"id":"T6825","span":{"begin":195,"end":200},"obj":"MD"},{"id":"T6826","span":{"begin":201,"end":208},"obj":"VB"},{"id":"T6827","span":{"begin":209,"end":214},"obj":"NNP"},{"id":"T6828","span":{"begin":215,"end":219},"obj":"IN"},{"id":"T6829","span":{"begin":220,"end":231},"obj":"NN"},{"id":"T6830","span":{"begin":231,"end":232},"obj":"."},{"id":"T6831","span":{"begin":233,"end":238},"obj":"NNP"},{"id":"T6832","span":{"begin":239,"end":244},"obj":"NNS"},{"id":"T6833","span":{"begin":244,"end":245},"obj":","},{"id":"T6834","span":{"begin":246,"end":251},"obj":"WDT"},{"id":"T6835","span":{"begin":252,"end":256},"obj":"VBP"},{"id":"T6836","span":{"begin":257,"end":261},"obj":"VBN"},{"id":"T6837","span":{"begin":262,"end":271},"obj":"VBN"},{"id":"T6838","span":{"begin":272,"end":274},"obj":"TO"},{"id":"T6839","span":{"begin":275,"end":282},"obj":"VB"},{"id":"T6840","span":{"begin":283,"end":288},"obj":"NNP"},{"id":"T6841","span":{"begin":289,"end":293},"obj":"NNP"},{"id":"T6842","span":{"begin":294,"end":306},"obj":"RB"},{"id":"T6843","span":{"begin":307,"end":308},"obj":"NNP"},{"id":"T6844","span":{"begin":308,"end":310},"obj":"CD"},{"id":"T6845","span":{"begin":310,"end":311},"obj":"NNP"},{"id":"T6846","span":{"begin":311,"end":312},"obj":","},{"id":"T6847","span":{"begin":313,"end":316},"obj":"CC"},{"id":"T6848","span":{"begin":317,"end":320},"obj":"DT"},{"id":"T6849","span":{"begin":321,"end":322},"obj":"NNP"},{"id":"T6850","span":{"begin":323,"end":327},"obj":"NN"},{"id":"T6851","span":{"begin":328,"end":332},"obj":"NN"},{"id":"T6852","span":{"begin":333,"end":339},"obj":"NNP"},{"id":"T6853","span":{"begin":339,"end":340},"obj":","},{"id":"T6854","span":{"begin":341,"end":347},"obj":"VBD"},{"id":"T6855","span":{"begin":348,"end":350},"obj":"IN"},{"id":"T6856","span":{"begin":351,"end":359},"obj":"JJ"},{"id":"T6857","span":{"begin":360,"end":363},"obj":"CC"},{"id":"T6858","span":{"begin":364,"end":372},"obj":"JJ"},{"id":"T6859","span":{"begin":373,"end":381},"obj":"NNS"},{"id":"T6860","span":{"begin":381,"end":382},"obj":","},{"id":"T6861","span":{"begin":383,"end":395},"obj":"RB"},{"id":"T6862","span":{"begin":395,"end":396},"obj":"."},{"id":"T6863","span":{"begin":397,"end":407},"obj":"NNP"},{"id":"T6864","span":{"begin":408,"end":413},"obj":"NNP"},{"id":"T6865","span":{"begin":414,"end":418},"obj":"NNP"},{"id":"T6866","span":{"begin":419,"end":425},"obj":"NNS"},{"id":"T6867","span":{"begin":426,"end":428},"obj":"IN"},{"id":"T6868","span":{"begin":429,"end":435},"obj":"JJ"},{"id":"T6869","span":{"begin":436,"end":437},"obj":"NNP"},{"id":"T6870","span":{"begin":438,"end":443},"obj":"NNS"},{"id":"T6871","span":{"begin":444,"end":448},"obj":"VBD"},{"id":"T6872","span":{"begin":449,"end":459},"obj":"VBN"},{"id":"T6873","span":{"begin":460,"end":462},"obj":"IN"},{"id":"T6874","span":{"begin":463,"end":472},"obj":"JJ"},{"id":"T6875","span":{"begin":473,"end":479},"obj":"NN"},{"id":"T6876","span":{"begin":480,"end":485},"obj":"IN"},{"id":"T6877","span":{"begin":486,"end":497},"obj":"NN"},{"id":"T6878","span":{"begin":498,"end":502},"obj":"IN"},{"id":"T6879","span":{"begin":503,"end":511},"obj":"JJ"},{"id":"T6880","span":{"begin":512,"end":515},"obj":"IN"},{"id":"T6881","span":{"begin":516,"end":525},"obj":"JJ"},{"id":"T6882","span":{"begin":526,"end":530},"obj":"NN"},{"id":"T6883","span":{"begin":531,"end":537},"obj":"NNS"},{"id":"T6884","span":{"begin":537,"end":538},"obj":"."},{"id":"T6885","span":{"begin":539,"end":552},"obj":"RB"},{"id":"T6886","span":{"begin":552,"end":553},"obj":","},{"id":"T6887","span":{"begin":554,"end":557},"obj":"DT"},{"id":"T6888","span":{"begin":558,"end":571},"obj":"NN"},{"id":"T6889","span":{"begin":572,"end":574},"obj":"IN"},{"id":"T6890","span":{"begin":575,"end":580},"obj":"NNP"},{"id":"T6891","span":{"begin":581,"end":585},"obj":"NNP"},{"id":"T6892","span":{"begin":586,"end":589},"obj":"VBD"},{"id":"T6893","span":{"begin":590,"end":604},"obj":"JJ"},{"id":"T6894","span":{"begin":605,"end":607},"obj":"IN"},{"id":"T6895","span":{"begin":608,"end":610},"obj":"DT"},{"id":"T6896","span":{"begin":611,"end":619},"obj":"JJ"},{"id":"T6897","span":{"begin":620,"end":632},"obj":"JJ"},{"id":"T6898","span":{"begin":633,"end":642},"obj":"JJ"},{"id":"T6899","span":{"begin":643,"end":647},"obj":"NN"},{"id":"T6900","span":{"begin":648,"end":652},"obj":"IN"},{"id":"T6901","span":{"begin":653,"end":660},"obj":"JJ"},{"id":"T6902","span":{"begin":661,"end":673},"obj":"NN"},{"id":"T6903","span":{"begin":674,"end":678},"obj":"CD"},{"id":"T6904","span":{"begin":679,"end":684},"obj":"NNS"},{"id":"T6905","span":{"begin":685,"end":690},"obj":"IN"},{"id":"T6906","span":{"begin":691,"end":694},"obj":"DT"},{"id":"T6907","span":{"begin":695,"end":703},"obj":"NN"},{"id":"T6908","span":{"begin":704,"end":706},"obj":"IN"},{"id":"T6909","span":{"begin":707,"end":715},"obj":"JJ"},{"id":"T6910","span":{"begin":715,"end":716},"obj":"."},{"id":"T6911","span":{"begin":717,"end":720},"obj":"DT"},{"id":"T6912","span":{"begin":721,"end":726},"obj":"NN"},{"id":"T6913","span":{"begin":727,"end":729},"obj":"IN"},{"id":"T6914","span":{"begin":730,"end":735},"obj":"NNP"},{"id":"T6915","span":{"begin":736,"end":740},"obj":"NNP"},{"id":"T6916","span":{"begin":741,"end":744},"obj":"VBD"},{"id":"T6917","span":{"begin":745,"end":749},"obj":"RB"},{"id":"T6918","span":{"begin":750,"end":752},"obj":"TO"},{"id":"T6919","span":{"begin":753,"end":761},"obj":"NN"},{"id":"T6920","span":{"begin":762,"end":767},"obj":"IN"},{"id":"T6921","span":{"begin":768,"end":770},"obj":"CD"},{"id":"T6922","span":{"begin":771,"end":776},"obj":"NNS"},{"id":"T6923","span":{"begin":777,"end":781},"obj":"IN"},{"id":"T6924","span":{"begin":782,"end":793},"obj":"NN"},{"id":"T6925","span":{"begin":794,"end":795},"obj":"-LRB-"},{"id":"T6926","span":{"begin":795,"end":801},"obj":"NN"},{"id":"T6927","span":{"begin":802,"end":804},"obj":"CD"},{"id":"T6928","span":{"begin":804,"end":805},"obj":"-RRB-"},{"id":"T6929","span":{"begin":805,"end":806},"obj":"."},{"id":"T6930","span":{"begin":807,"end":818},"obj":"RB"},{"id":"T6931","span":{"begin":818,"end":819},"obj":","},{"id":"T6932","span":{"begin":820,"end":824},"obj":"DT"},{"id":"T6933","span":{"begin":825,"end":828},"obj":"NNP"},{"id":"T6934","span":{"begin":829,"end":832},"obj":"CC"},{"id":"T6935","span":{"begin":833,"end":838},"obj":"JJ"},{"id":"T6936","span":{"begin":839,"end":847},"obj":"JJ"},{"id":"T6937","span":{"begin":848,"end":855},"obj":"JJ"},{"id":"T6938","span":{"begin":856,"end":867},"obj":"JJ"},{"id":"T6939","span":{"begin":868,"end":880},"obj":"NN"},{"id":"T6940","span":{"begin":881,"end":883},"obj":"IN"},{"id":"T6941","span":{"begin":884,"end":889},"obj":"NNP"},{"id":"T6942","span":{"begin":890,"end":894},"obj":"NNP"},{"id":"T6943","span":{"begin":895,"end":896},"obj":"-LRB-"},{"id":"T6944","span":{"begin":896,"end":902},"obj":"NNP"},{"id":"T6945","span":{"begin":903,"end":905},"obj":"CD"},{"id":"T6946","span":{"begin":905,"end":906},"obj":"-RRB-"},{"id":"T6947","span":{"begin":906,"end":907},"obj":"."},{"id":"T6948","span":{"begin":908,"end":921},"obj":"RB"},{"id":"T6949","span":{"begin":921,"end":922},"obj":","},{"id":"T6950","span":{"begin":923,"end":925},"obj":"IN"},{"id":"T6951","span":{"begin":926,"end":927},"obj":"DT"},{"id":"T6952","span":{"begin":928,"end":936},"obj":"JJ"},{"id":"T6953","span":{"begin":937,"end":942},"obj":"NN"},{"id":"T6954","span":{"begin":943,"end":945},"obj":"PRP"},{"id":"T6955","span":{"begin":946,"end":950},"obj":"VBP"},{"id":"T6956","span":{"begin":951,"end":956},"obj":"VBN"},{"id":"T6957","span":{"begin":957,"end":961},"obj":"IN"},{"id":"T6958","span":{"begin":962,"end":968},"obj":"JJ"},{"id":"T6959","span":{"begin":969,"end":974},"obj":"JJ"},{"id":"T6960","span":{"begin":975,"end":976},"obj":"NNP"},{"id":"T6961","span":{"begin":977,"end":982},"obj":"NNS"},{"id":"T6962","span":{"begin":983,"end":985},"obj":"VBP"},{"id":"T6963","span":{"begin":986,"end":989},"obj":"RB"},{"id":"T6964","span":{"begin":990,"end":997},"obj":"VB"},{"id":"T6965","span":{"begin":998,"end":1003},"obj":"NNP"},{"id":"T6966","span":{"begin":1004,"end":1008},"obj":"NNP"},{"id":"T6967","span":{"begin":1009,"end":1014},"obj":"IN"},{"id":"T6968","span":{"begin":1015,"end":1025},"obj":"NN"},{"id":"T6969","span":{"begin":1026,"end":1027},"obj":"-LRB-"},{"id":"T6970","span":{"begin":1027,"end":1036},"obj":"NNP"},{"id":"T6971","span":{"begin":1037,"end":1039},"obj":"FW"},{"id":"T6972","span":{"begin":1040,"end":1042},"obj":"FW"},{"id":"T6973","span":{"begin":1042,"end":1043},"obj":","},{"id":"T6974","span":{"begin":1044,"end":1054},"obj":"NN"},{"id":"T6975","span":{"begin":1055,"end":1057},"obj":"IN"},{"id":"T6976","span":{"begin":1058,"end":1069},"obj":"NN"},{"id":"T6977","span":{"begin":1069,"end":1070},"obj":"-RRB-"},{"id":"T6978","span":{"begin":1070,"end":1071},"obj":"."},{"id":"T6979","span":{"begin":1072,"end":1076},"obj":"JJ"},{"id":"T6980","span":{"begin":1076,"end":1077},"obj":","},{"id":"T6981","span":{"begin":1078,"end":1080},"obj":"PRP"},{"id":"T6982","span":{"begin":1081,"end":1087},"obj":"VBD"},{"id":"T6983","span":{"begin":1088,"end":1090},"obj":"TO"},{"id":"T6984","span":{"begin":1091,"end":1097},"obj":"VB"},{"id":"T6985","span":{"begin":1098,"end":1103},"obj":"NNP"},{"id":"T6986","span":{"begin":1104,"end":1111},"obj":"NN"},{"id":"T6987","span":{"begin":1112,"end":1114},"obj":"IN"},{"id":"T6988","span":{"begin":1115,"end":1121},"obj":"JJ"},{"id":"T6989","span":{"begin":1122,"end":1129},"obj":"NNS"},{"id":"T6990","span":{"begin":1130,"end":1133},"obj":"CC"},{"id":"T6991","span":{"begin":1134,"end":1140},"obj":"VBN"},{"id":"T6992","span":{"begin":1141,"end":1148},"obj":"JJ"},{"id":"T6993","span":{"begin":1149,"end":1161},"obj":"RB"},{"id":"T6994","span":{"begin":1162,"end":1171},"obj":"JJ"},{"id":"T6995","span":{"begin":1172,"end":1182},"obj":"NNS"},{"id":"T6996","span":{"begin":1182,"end":1183},"obj":"."},{"id":"T6997","span":{"begin":1184,"end":1191},"obj":"RB"},{"id":"T6998","span":{"begin":1191,"end":1192},"obj":","},{"id":"T6999","span":{"begin":1193,"end":1195},"obj":"IN"},{"id":"T7000","span":{"begin":1196,"end":1199},"obj":"PRP$"},{"id":"T7001","span":{"begin":1200,"end":1205},"obj":"NNS"},{"id":"T7002","span":{"begin":1206,"end":1211},"obj":"DT"},{"id":"T7003","span":{"begin":1212,"end":1222},"obj":"JJ"},{"id":"T7004","span":{"begin":1223,"end":1233},"obj":"NNS"},{"id":"T7005","span":{"begin":1234,"end":1237},"obj":"VBD"},{"id":"T7006","span":{"begin":1238,"end":1242},"obj":"RB"},{"id":"T7007","span":{"begin":1243,"end":1252},"obj":"VB"},{"id":"T7008","span":{"begin":1253,"end":1256},"obj":"DT"},{"id":"T7009","span":{"begin":1257,"end":1268},"obj":"JJ"},{"id":"T7010","span":{"begin":1269,"end":1274},"obj":"NNP"},{"id":"T7011","span":{"begin":1275,"end":1282},"obj":"NN"},{"id":"T7012","span":{"begin":1283,"end":1286},"obj":"CC"},{"id":"T7013","span":{"begin":1287,"end":1290},"obj":"RB"},{"id":"T7014","span":{"begin":1291,"end":1294},"obj":"DT"},{"id":"T7015","span":{"begin":1295,"end":1301},"obj":"JJ"},{"id":"T7016","span":{"begin":1302,"end":1309},"obj":"NN"},{"id":"T7017","span":{"begin":1309,"end":1310},"obj":"."}],"relations":[{"id":"R5311","pred":"compound","subj":"T6787","obj":"T6788"},{"id":"R5312","pred":"nsubj","subj":"T6788","obj":"T6816"},{"id":"R5313","pred":"prep","subj":"T6789","obj":"T6788"},{"id":"R5314","pred":"amod","subj":"T6790","obj":"T6791"},{"id":"R5315","pred":"pobj","subj":"T6791","obj":"T6789"},{"id":"R5316","pred":"det","subj":"T6792","obj":"T6793"},{"id":"R5317","pred":"nsubj","subj":"T6793","obj":"T6816"},{"id":"R5318","pred":"mark","subj":"T6794","obj":"T6798"},{"id":"R5319","pred":"nsubjpass","subj":"T6795","obj":"T6798"},{"id":"R5320","pred":"aux","subj":"T6796","obj":"T6798"},{"id":"R5321","pred":"auxpass","subj":"T6797","obj":"T6798"},{"id":"R5322","pred":"acl","subj":"T6798","obj":"T6793"},{"id":"R5323","pred":"aux","subj":"T6799","obj":"T6800"},{"id":"R5324","pred":"xcomp","subj":"T6800","obj":"T6798"},{"id":"R5325","pred":"prep","subj":"T6801","obj":"T6800"},{"id":"R5326","pred":"det","subj":"T6802","obj":"T6804"},{"id":"R5327","pred":"compound","subj":"T6803","obj":"T6804"},{"id":"R5328","pred":"pobj","subj":"T6804","obj":"T6801"},{"id":"R5329","pred":"prep","subj":"T6805","obj":"T6804"},{"id":"R5330","pred":"pobj","subj":"T6806","obj":"T6805"},{"id":"R5331","pred":"nmod","subj":"T6807","obj":"T6809"},{"id":"R5332","pred":"nummod","subj":"T6808","obj":"T6809"},{"id":"R5333","pred":"nsubj","subj":"T6809","obj":"T6816"},{"id":"R5334","pred":"cc","subj":"T6810","obj":"T6809"},{"id":"R5335","pred":"compound","subj":"T6811","obj":"T6812"},{"id":"R5336","pred":"conj","subj":"T6812","obj":"T6809"},{"id":"R5337","pred":"nummod","subj":"T6813","obj":"T6814"},{"id":"R5338","pred":"appos","subj":"T6814","obj":"T6812"},{"id":"R5339","pred":"punct","subj":"T6815","obj":"T6816"},{"id":"R5340","pred":"ROOT","subj":"T6816","obj":"T6816"},{"id":"R5341","pred":"dobj","subj":"T6817","obj":"T6816"},{"id":"R5342","pred":"aux","subj":"T6818","obj":"T6819"},{"id":"R5343","pred":"xcomp","subj":"T6819","obj":"T6816"},{"id":"R5344","pred":"mark","subj":"T6820","obj":"T6826"},{"id":"R5345","pred":"amod","subj":"T6821","obj":"T6824"},{"id":"R5346","pred":"compound","subj":"T6822","obj":"T6823"},{"id":"R5347","pred":"compound","subj":"T6823","obj":"T6824"},{"id":"R5348","pred":"nsubj","subj":"T6824","obj":"T6826"},{"id":"R5349","pred":"aux","subj":"T6825","obj":"T6826"},{"id":"R5350","pred":"ccomp","subj":"T6826","obj":"T6819"},{"id":"R5351","pred":"dobj","subj":"T6827","obj":"T6826"},{"id":"R5352","pred":"prep","subj":"T6828","obj":"T6826"},{"id":"R5353","pred":"pobj","subj":"T6829","obj":"T6828"},{"id":"R5354","pred":"punct","subj":"T6830","obj":"T6816"},{"id":"R5355","pred":"compound","subj":"T6831","obj":"T6832"},{"id":"R5356","pred":"nsubj","subj":"T6832","obj":"T6854"},{"id":"R5357","pred":"punct","subj":"T6833","obj":"T6832"},{"id":"R5358","pred":"nsubjpass","subj":"T6834","obj":"T6837"},{"id":"R5359","pred":"aux","subj":"T6835","obj":"T6837"},{"id":"R5360","pred":"auxpass","subj":"T6836","obj":"T6837"},{"id":"R5361","pred":"relcl","subj":"T6837","obj":"T6832"},{"id":"R5362","pred":"aux","subj":"T6838","obj":"T6839"},{"id":"R5363","pred":"xcomp","subj":"T6839","obj":"T6837"},{"id":"R5364","pred":"compound","subj":"T6840","obj":"T6841"},{"id":"R5365","pred":"dobj","subj":"T6841","obj":"T6839"},{"id":"R5366","pred":"compound","subj":"T6842","obj":"T6843"},{"id":"R5367","pred":"appos","subj":"T6843","obj":"T6841"},{"id":"R5368","pred":"nummod","subj":"T6844","obj":"T6845"},{"id":"R5369","pred":"conj","subj":"T6845","obj":"T6841"},{"id":"R5370","pred":"punct","subj":"T6846","obj":"T6845"},{"id":"R5371","pred":"cc","subj":"T6847","obj":"T6845"},{"id":"R5372","pred":"det","subj":"T6848","obj":"T6851"},{"id":"R5373","pred":"compound","subj":"T6849","obj":"T6850"},{"id":"R5374","pred":"compound","subj":"T6850","obj":"T6851"},{"id":"R5375","pred":"compound","subj":"T6851","obj":"T6852"},{"id":"R5376","pred":"conj","subj":"T6852","obj":"T6845"},{"id":"R5377","pred":"punct","subj":"T6853","obj":"T6832"},{"id":"R5378","pred":"ROOT","subj":"T6854","obj":"T6854"},{"id":"R5379","pred":"prep","subj":"T6855","obj":"T6854"},{"id":"R5380","pred":"amod","subj":"T6856","obj":"T6859"},{"id":"R5381","pred":"cc","subj":"T6857","obj":"T6856"},{"id":"R5382","pred":"conj","subj":"T6858","obj":"T6856"},{"id":"R5383","pred":"pobj","subj":"T6859","obj":"T6855"},{"id":"R5384","pred":"punct","subj":"T6860","obj":"T6854"},{"id":"R5385","pred":"advmod","subj":"T6861","obj":"T6854"},{"id":"R5386","pred":"punct","subj":"T6862","obj":"T6854"},{"id":"R5387","pred":"compound","subj":"T6863","obj":"T6865"},{"id":"R5388","pred":"compound","subj":"T6864","obj":"T6865"},{"id":"R5389","pred":"compound","subj":"T6865","obj":"T6866"},{"id":"R5390","pred":"nsubjpass","subj":"T6866","obj":"T6872"},{"id":"R5391","pred":"prep","subj":"T6867","obj":"T6866"},{"id":"R5392","pred":"amod","subj":"T6868","obj":"T6870"},{"id":"R5393","pred":"compound","subj":"T6869","obj":"T6870"},{"id":"R5394","pred":"pobj","subj":"T6870","obj":"T6867"},{"id":"R5395","pred":"auxpass","subj":"T6871","obj":"T6872"},{"id":"R5396","pred":"ROOT","subj":"T6872","obj":"T6872"},{"id":"R5397","pred":"agent","subj":"T6873","obj":"T6872"},{"id":"R5398","pred":"amod","subj":"T6874","obj":"T6875"},{"id":"R5399","pred":"pobj","subj":"T6875","obj":"T6873"},{"id":"R5400","pred":"prep","subj":"T6876","obj":"T6872"},{"id":"R5401","pred":"pobj","subj":"T6877","obj":"T6876"},{"id":"R5402","pred":"prep","subj":"T6878","obj":"T6877"},{"id":"R5403","pred":"pobj","subj":"T6879","obj":"T6878"},{"id":"R5404","pred":"prep","subj":"T6880","obj":"T6872"},{"id":"R5405","pred":"amod","subj":"T6881","obj":"T6883"},{"id":"R5406","pred":"compound","subj":"T6882","obj":"T6883"},{"id":"R5407","pred":"pobj","subj":"T6883","obj":"T6880"},{"id":"R5408","pred":"punct","subj":"T6884","obj":"T6872"},{"id":"R5409","pred":"advmod","subj":"T6885","obj":"T6892"},{"id":"R5410","pred":"punct","subj":"T6886","obj":"T6892"},{"id":"R5411","pred":"det","subj":"T6887","obj":"T6888"},{"id":"R5412","pred":"nsubj","subj":"T6888","obj":"T6892"},{"id":"R5413","pred":"prep","subj":"T6889","obj":"T6888"},{"id":"R5414","pred":"compound","subj":"T6890","obj":"T6891"},{"id":"R5415","pred":"pobj","subj":"T6891","obj":"T6889"},{"id":"R5416","pred":"ROOT","subj":"T6892","obj":"T6892"},{"id":"R5417","pred":"acomp","subj":"T6893","obj":"T6892"},{"id":"R5418","pred":"prep","subj":"T6894","obj":"T6893"},{"id":"R5419","pred":"det","subj":"T6895","obj":"T6899"},{"id":"R5420","pred":"amod","subj":"T6896","obj":"T6899"},{"id":"R5421","pred":"amod","subj":"T6897","obj":"T6899"},{"id":"R5422","pred":"amod","subj":"T6898","obj":"T6899"},{"id":"R5423","pred":"pobj","subj":"T6899","obj":"T6894"},{"id":"R5424","pred":"prep","subj":"T6900","obj":"T6893"},{"id":"R5425","pred":"amod","subj":"T6901","obj":"T6902"},{"id":"R5426","pred":"pobj","subj":"T6902","obj":"T6900"},{"id":"R5427","pred":"nummod","subj":"T6903","obj":"T6904"},{"id":"R5428","pred":"npadvmod","subj":"T6904","obj":"T6905"},{"id":"R5429","pred":"prep","subj":"T6905","obj":"T6892"},{"id":"R5430","pred":"det","subj":"T6906","obj":"T6907"},{"id":"R5431","pred":"pobj","subj":"T6907","obj":"T6905"},{"id":"R5432","pred":"prep","subj":"T6908","obj":"T6907"},{"id":"R5433","pred":"pobj","subj":"T6909","obj":"T6908"},{"id":"R5434","pred":"punct","subj":"T6910","obj":"T6892"},{"id":"R5435","pred":"det","subj":"T6911","obj":"T6912"},{"id":"R5436","pred":"nsubj","subj":"T6912","obj":"T6916"},{"id":"R5437","pred":"prep","subj":"T6913","obj":"T6912"},{"id":"R5438","pred":"compound","subj":"T6914","obj":"T6915"},{"id":"R5439","pred":"pobj","subj":"T6915","obj":"T6913"},{"id":"R5440","pred":"ROOT","subj":"T6916","obj":"T6916"},{"id":"R5441","pred":"advmod","subj":"T6917","obj":"T6916"},{"id":"R5442","pred":"prep","subj":"T6918","obj":"T6917"},{"id":"R5443","pred":"pobj","subj":"T6919","obj":"T6918"},{"id":"R5444","pred":"prep","subj":"T6920","obj":"T6916"},{"id":"R5445","pred":"nummod","subj":"T6921","obj":"T6922"},{"id":"R5446","pred":"pobj","subj":"T6922","obj":"T6920"},{"id":"R5447","pred":"prep","subj":"T6923","obj":"T6922"},{"id":"R5448","pred":"pobj","subj":"T6924","obj":"T6923"},{"id":"R5449","pred":"punct","subj":"T6925","obj":"T6926"},{"id":"R5450","pred":"appos","subj":"T6926","obj":"T6922"},{"id":"R5451","pred":"nummod","subj":"T6927","obj":"T6926"},{"id":"R5452","pred":"punct","subj":"T6928","obj":"T6916"},{"id":"R5453","pred":"punct","subj":"T6929","obj":"T6916"},{"id":"R5454","pred":"advmod","subj":"T6930","obj":"T6937"},{"id":"R5455","pred":"punct","subj":"T6931","obj":"T6937"},{"id":"R5456","pred":"preconj","subj":"T6932","obj":"T6933"},{"id":"R5457","pred":"nsubj","subj":"T6933","obj":"T6937"},{"id":"R5458","pred":"cc","subj":"T6934","obj":"T6933"},{"id":"R5459","pred":"nsubj","subj":"T6935","obj":"T6937"},{"id":"R5460","pred":"nsubj","subj":"T6936","obj":"T6937"},{"id":"R5461","pred":"ROOT","subj":"T6937","obj":"T6937"},{"id":"R5462","pred":"amod","subj":"T6938","obj":"T6939"},{"id":"R5463","pred":"dobj","subj":"T6939","obj":"T6937"},{"id":"R5464","pred":"prep","subj":"T6940","obj":"T6939"},{"id":"R5465","pred":"compound","subj":"T6941","obj":"T6942"},{"id":"R5466","pred":"pobj","subj":"T6942","obj":"T6940"},{"id":"R5467","pred":"punct","subj":"T6943","obj":"T6944"},{"id":"R5468","pred":"appos","subj":"T6944","obj":"T6942"},{"id":"R5469","pred":"nummod","subj":"T6945","obj":"T6944"},{"id":"R5470","pred":"punct","subj":"T6946","obj":"T6942"},{"id":"R5471","pred":"punct","subj":"T6947","obj":"T6937"},{"id":"R5472","pred":"advmod","subj":"T6948","obj":"T6956"},{"id":"R5473","pred":"punct","subj":"T6949","obj":"T6956"},{"id":"R5474","pred":"prep","subj":"T6950","obj":"T6956"},{"id":"R5475","pred":"det","subj":"T6951","obj":"T6953"},{"id":"R5476","pred":"amod","subj":"T6952","obj":"T6953"},{"id":"R5477","pred":"pobj","subj":"T6953","obj":"T6950"},{"id":"R5478","pred":"nsubj","subj":"T6954","obj":"T6956"},{"id":"R5479","pred":"aux","subj":"T6955","obj":"T6956"},{"id":"R5480","pred":"ROOT","subj":"T6956","obj":"T6956"},{"id":"R5481","pred":"mark","subj":"T6957","obj":"T6964"},{"id":"R5482","pred":"amod","subj":"T6958","obj":"T6961"},{"id":"R5483","pred":"compound","subj":"T6959","obj":"T6960"},{"id":"R5484","pred":"compound","subj":"T6960","obj":"T6961"},{"id":"R5485","pred":"nsubj","subj":"T6961","obj":"T6964"},{"id":"R5486","pred":"aux","subj":"T6962","obj":"T6964"},{"id":"R5487","pred":"neg","subj":"T6963","obj":"T6964"},{"id":"R5488","pred":"ccomp","subj":"T6964","obj":"T6956"},{"id":"R5489","pred":"compound","subj":"T6965","obj":"T6966"},{"id":"R5490","pred":"dobj","subj":"T6966","obj":"T6964"},{"id":"R5491","pred":"prep","subj":"T6967","obj":"T6964"},{"id":"R5492","pred":"pobj","subj":"T6968","obj":"T6967"},{"id":"R5493","pred":"punct","subj":"T6969","obj":"T6968"},{"id":"R5494","pred":"nmod","subj":"T6970","obj":"T6972"},{"id":"R5495","pred":"nmod","subj":"T6971","obj":"T6972"},{"id":"R5496","pred":"appos","subj":"T6972","obj":"T6968"},{"id":"R5497","pred":"punct","subj":"T6973","obj":"T6968"},{"id":"R5498","pred":"conj","subj":"T6974","obj":"T6964"},{"id":"R5499","pred":"prep","subj":"T6975","obj":"T6974"},{"id":"R5500","pred":"pobj","subj":"T6976","obj":"T6975"},{"id":"R5501","pred":"punct","subj":"T6977","obj":"T6956"},{"id":"R5502","pred":"punct","subj":"T6978","obj":"T6956"},{"id":"R5503","pred":"advmod","subj":"T6979","obj":"T6982"},{"id":"R5504","pred":"punct","subj":"T6980","obj":"T6982"},{"id":"R5505","pred":"nsubj","subj":"T6981","obj":"T6982"},{"id":"R5506","pred":"ROOT","subj":"T6982","obj":"T6982"},{"id":"R5507","pred":"aux","subj":"T6983","obj":"T6984"},{"id":"R5508","pred":"xcomp","subj":"T6984","obj":"T6982"},{"id":"R5509","pred":"compound","subj":"T6985","obj":"T6986"},{"id":"R5510","pred":"dobj","subj":"T6986","obj":"T6984"},{"id":"R5511","pred":"prep","subj":"T6987","obj":"T6984"},{"id":"R5512","pred":"amod","subj":"T6988","obj":"T6989"},{"id":"R5513","pred":"pobj","subj":"T6989","obj":"T6987"},{"id":"R5514","pred":"cc","subj":"T6990","obj":"T6982"},{"id":"R5515","pred":"conj","subj":"T6991","obj":"T6982"},{"id":"R5516","pred":"amod","subj":"T6992","obj":"T6995"},{"id":"R5517","pred":"advmod","subj":"T6993","obj":"T6994"},{"id":"R5518","pred":"amod","subj":"T6994","obj":"T6995"},{"id":"R5519","pred":"dobj","subj":"T6995","obj":"T6991"},{"id":"R5520","pred":"punct","subj":"T6996","obj":"T6982"},{"id":"R5521","pred":"advmod","subj":"T6997","obj":"T7007"},{"id":"R5522","pred":"punct","subj":"T6998","obj":"T7007"},{"id":"R5523","pred":"prep","subj":"T6999","obj":"T7007"},{"id":"R5524","pred":"poss","subj":"T7000","obj":"T7001"},{"id":"R5525","pred":"pobj","subj":"T7001","obj":"T6999"},{"id":"R5526","pred":"det","subj":"T7002","obj":"T7004"},{"id":"R5527","pred":"amod","subj":"T7003","obj":"T7004"},{"id":"R5528","pred":"nsubj","subj":"T7004","obj":"T7007"},{"id":"R5529","pred":"aux","subj":"T7005","obj":"T7007"},{"id":"R5530","pred":"advmod","subj":"T7006","obj":"T7007"},{"id":"R5531","pred":"ROOT","subj":"T7007","obj":"T7007"},{"id":"R5532","pred":"det","subj":"T7008","obj":"T7011"},{"id":"R5533","pred":"amod","subj":"T7009","obj":"T7011"},{"id":"R5534","pred":"compound","subj":"T7010","obj":"T7011"},{"id":"R5535","pred":"dobj","subj":"T7011","obj":"T7007"},{"id":"R5536","pred":"cc","subj":"T7012","obj":"T7011"},{"id":"R5537","pred":"neg","subj":"T7013","obj":"T7016"},{"id":"R5538","pred":"det","subj":"T7014","obj":"T7016"},{"id":"R5539","pred":"amod","subj":"T7015","obj":"T7016"},{"id":"R5540","pred":"conj","subj":"T7016","obj":"T7011"},{"id":"R5541","pred":"punct","subj":"T7017","obj":"T7007"}],"text":"BMP-6 production in B cells\nThe fact that BMP-6 has been reported to act as an autocrine stimulator in chondrocytes [18] and ovarium [19], prompted us to investigate whether normal human B cells could produce BMP-6 upon stimulation. Ramos cells, which have been described to express BMP-6 mRNA endogenously [20], and the T cell line Jurkat, served as positive and negative controls, respectively. Endogenous BMP-6 mRNA levels in normal B cells were quantified by real-time RT-PCR after stimulation with anti-IgM for different time points. Interestingly, the up-regulation of BMP-6 mRNA was characteristic of an early-to intermediate inducible gene with maximal upregulation four hours after the addition of anti-IgM. The level of BMP-6 mRNA was back to baseline after 24 hours upon stimulation (Figure 10). Furthermore, both FCS and human AB-serum induced significant upregulation of BMP-6 mRNA (Figure 11). Interestingly, in a separate study we have found that normal human T cells do not express BMP-6 mRNA after activation (Sivertsen et al, manuscript in preparation). Next, we wanted to detect BMP-6 protein in normal B-cells and tested various commercially available antibodies. However, in our hands these anti-BMP-6 antibodies did only recognize the recombinant BMP-6 protein and not the native protein."}
UBERON-AE
{"project":"UBERON-AE","denotations":[{"id":"T6458","span":{"begin":842,"end":847},"obj":"http://purl.obolibrary.org/obo/UBERON_0001977"}],"text":"BMP-6 production in B cells\nThe fact that BMP-6 has been reported to act as an autocrine stimulator in chondrocytes [18] and ovarium [19], prompted us to investigate whether normal human B cells could produce BMP-6 upon stimulation. Ramos cells, which have been described to express BMP-6 mRNA endogenously [20], and the T cell line Jurkat, served as positive and negative controls, respectively. Endogenous BMP-6 mRNA levels in normal B cells were quantified by real-time RT-PCR after stimulation with anti-IgM for different time points. Interestingly, the up-regulation of BMP-6 mRNA was characteristic of an early-to intermediate inducible gene with maximal upregulation four hours after the addition of anti-IgM. The level of BMP-6 mRNA was back to baseline after 24 hours upon stimulation (Figure 10). Furthermore, both FCS and human AB-serum induced significant upregulation of BMP-6 mRNA (Figure 11). Interestingly, in a separate study we have found that normal human T cells do not express BMP-6 mRNA after activation (Sivertsen et al, manuscript in preparation). Next, we wanted to detect BMP-6 protein in normal B-cells and tested various commercially available antibodies. However, in our hands these anti-BMP-6 antibodies did only recognize the recombinant BMP-6 protein and not the native protein."}
GO-BP
{"project":"GO-BP","denotations":[{"id":"T6491","span":{"begin":473,"end":475},"obj":"http://purl.obolibrary.org/obo/GO_0003964"},{"id":"T6492","span":{"begin":561,"end":571},"obj":"http://purl.obolibrary.org/obo/GO_0065007"}],"text":"BMP-6 production in B cells\nThe fact that BMP-6 has been reported to act as an autocrine stimulator in chondrocytes [18] and ovarium [19], prompted us to investigate whether normal human B cells could produce BMP-6 upon stimulation. Ramos cells, which have been described to express BMP-6 mRNA endogenously [20], and the T cell line Jurkat, served as positive and negative controls, respectively. Endogenous BMP-6 mRNA levels in normal B cells were quantified by real-time RT-PCR after stimulation with anti-IgM for different time points. Interestingly, the up-regulation of BMP-6 mRNA was characteristic of an early-to intermediate inducible gene with maximal upregulation four hours after the addition of anti-IgM. The level of BMP-6 mRNA was back to baseline after 24 hours upon stimulation (Figure 10). Furthermore, both FCS and human AB-serum induced significant upregulation of BMP-6 mRNA (Figure 11). Interestingly, in a separate study we have found that normal human T cells do not express BMP-6 mRNA after activation (Sivertsen et al, manuscript in preparation). Next, we wanted to detect BMP-6 protein in normal B-cells and tested various commercially available antibodies. However, in our hands these anti-BMP-6 antibodies did only recognize the recombinant BMP-6 protein and not the native protein."}
GO-MF
{"project":"GO-MF","denotations":[{"id":"T7018","span":{"begin":473,"end":475},"obj":"http://purl.obolibrary.org/obo/GO_0003964"},{"id":"T7019","span":{"begin":1172,"end":1182},"obj":"http://purl.obolibrary.org/obo/GO_0003823"},{"id":"T7020","span":{"begin":1223,"end":1233},"obj":"http://purl.obolibrary.org/obo/GO_0003823"}],"text":"BMP-6 production in B cells\nThe fact that BMP-6 has been reported to act as an autocrine stimulator in chondrocytes [18] and ovarium [19], prompted us to investigate whether normal human B cells could produce BMP-6 upon stimulation. Ramos cells, which have been described to express BMP-6 mRNA endogenously [20], and the T cell line Jurkat, served as positive and negative controls, respectively. Endogenous BMP-6 mRNA levels in normal B cells were quantified by real-time RT-PCR after stimulation with anti-IgM for different time points. Interestingly, the up-regulation of BMP-6 mRNA was characteristic of an early-to intermediate inducible gene with maximal upregulation four hours after the addition of anti-IgM. The level of BMP-6 mRNA was back to baseline after 24 hours upon stimulation (Figure 10). Furthermore, both FCS and human AB-serum induced significant upregulation of BMP-6 mRNA (Figure 11). Interestingly, in a separate study we have found that normal human T cells do not express BMP-6 mRNA after activation (Sivertsen et al, manuscript in preparation). Next, we wanted to detect BMP-6 protein in normal B-cells and tested various commercially available antibodies. However, in our hands these anti-BMP-6 antibodies did only recognize the recombinant BMP-6 protein and not the native protein."}
GO-CC
{"project":"GO-CC","denotations":[{"id":"T7021","span":{"begin":22,"end":27},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T7022","span":{"begin":189,"end":194},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T7023","span":{"begin":239,"end":244},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T7024","span":{"begin":438,"end":443},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T7025","span":{"begin":977,"end":982},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T7026","span":{"begin":1124,"end":1129},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T7027","span":{"begin":1172,"end":1182},"obj":"http://purl.obolibrary.org/obo/GO_0019815"},{"id":"T7028","span":{"begin":1223,"end":1233},"obj":"http://purl.obolibrary.org/obo/GO_0019815"},{"id":"T7029","span":{"begin":1172,"end":1182},"obj":"http://purl.obolibrary.org/obo/GO_0042571"},{"id":"T7030","span":{"begin":1223,"end":1233},"obj":"http://purl.obolibrary.org/obo/GO_0042571"}],"text":"BMP-6 production in B cells\nThe fact that BMP-6 has been reported to act as an autocrine stimulator in chondrocytes [18] and ovarium [19], prompted us to investigate whether normal human B cells could produce BMP-6 upon stimulation. Ramos cells, which have been described to express BMP-6 mRNA endogenously [20], and the T cell line Jurkat, served as positive and negative controls, respectively. Endogenous BMP-6 mRNA levels in normal B cells were quantified by real-time RT-PCR after stimulation with anti-IgM for different time points. Interestingly, the up-regulation of BMP-6 mRNA was characteristic of an early-to intermediate inducible gene with maximal upregulation four hours after the addition of anti-IgM. The level of BMP-6 mRNA was back to baseline after 24 hours upon stimulation (Figure 10). Furthermore, both FCS and human AB-serum induced significant upregulation of BMP-6 mRNA (Figure 11). Interestingly, in a separate study we have found that normal human T cells do not express BMP-6 mRNA after activation (Sivertsen et al, manuscript in preparation). Next, we wanted to detect BMP-6 protein in normal B-cells and tested various commercially available antibodies. However, in our hands these anti-BMP-6 antibodies did only recognize the recombinant BMP-6 protein and not the native protein."}
sentences
{"project":"sentences","denotations":[{"id":"T6481","span":{"begin":0,"end":27},"obj":"Sentence"},{"id":"T6482","span":{"begin":28,"end":232},"obj":"Sentence"},{"id":"T6483","span":{"begin":233,"end":396},"obj":"Sentence"},{"id":"T6484","span":{"begin":397,"end":538},"obj":"Sentence"},{"id":"T6485","span":{"begin":539,"end":716},"obj":"Sentence"},{"id":"T6486","span":{"begin":717,"end":806},"obj":"Sentence"},{"id":"T6487","span":{"begin":807,"end":907},"obj":"Sentence"},{"id":"T6488","span":{"begin":908,"end":1071},"obj":"Sentence"},{"id":"T6489","span":{"begin":1072,"end":1183},"obj":"Sentence"},{"id":"T6490","span":{"begin":1184,"end":1310},"obj":"Sentence"},{"id":"T84","span":{"begin":0,"end":27},"obj":"Sentence"},{"id":"T85","span":{"begin":28,"end":232},"obj":"Sentence"},{"id":"T86","span":{"begin":233,"end":396},"obj":"Sentence"},{"id":"T87","span":{"begin":397,"end":538},"obj":"Sentence"},{"id":"T88","span":{"begin":539,"end":716},"obj":"Sentence"},{"id":"T89","span":{"begin":717,"end":806},"obj":"Sentence"},{"id":"T90","span":{"begin":807,"end":907},"obj":"Sentence"},{"id":"T91","span":{"begin":908,"end":1071},"obj":"Sentence"},{"id":"T92","span":{"begin":1072,"end":1183},"obj":"Sentence"},{"id":"T93","span":{"begin":1184,"end":1310},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"BMP-6 production in B cells\nThe fact that BMP-6 has been reported to act as an autocrine stimulator in chondrocytes [18] and ovarium [19], prompted us to investigate whether normal human B cells could produce BMP-6 upon stimulation. Ramos cells, which have been described to express BMP-6 mRNA endogenously [20], and the T cell line Jurkat, served as positive and negative controls, respectively. Endogenous BMP-6 mRNA levels in normal B cells were quantified by real-time RT-PCR after stimulation with anti-IgM for different time points. Interestingly, the up-regulation of BMP-6 mRNA was characteristic of an early-to intermediate inducible gene with maximal upregulation four hours after the addition of anti-IgM. The level of BMP-6 mRNA was back to baseline after 24 hours upon stimulation (Figure 10). Furthermore, both FCS and human AB-serum induced significant upregulation of BMP-6 mRNA (Figure 11). Interestingly, in a separate study we have found that normal human T cells do not express BMP-6 mRNA after activation (Sivertsen et al, manuscript in preparation). Next, we wanted to detect BMP-6 protein in normal B-cells and tested various commercially available antibodies. However, in our hands these anti-BMP-6 antibodies did only recognize the recombinant BMP-6 protein and not the native protein."}
events-check-again
{"project":"events-check-again","denotations":[{"id":"T7087","span":{"begin":0,"end":5},"obj":"Protein"},{"id":"T7088","span":{"begin":6,"end":16},"obj":"Gene_expression"},{"id":"T7089","span":{"begin":42,"end":47},"obj":"Protein"},{"id":"T7090","span":{"begin":201,"end":208},"obj":"Gene_expression"},{"id":"T7091","span":{"begin":209,"end":214},"obj":"Protein"},{"id":"T7092","span":{"begin":275,"end":282},"obj":"Transcription"},{"id":"T7093","span":{"begin":283,"end":288},"obj":"Protein"},{"id":"T7094","span":{"begin":408,"end":413},"obj":"Protein"},{"id":"T7095","span":{"begin":558,"end":571},"obj":"Positive_regulation"},{"id":"T7096","span":{"begin":575,"end":580},"obj":"Protein"},{"id":"T7097","span":{"begin":661,"end":673},"obj":"Positive_regulation"},{"id":"T7098","span":{"begin":730,"end":735},"obj":"Protein"},{"id":"T7099","span":{"begin":745,"end":761},"obj":"Negative_regulation"},{"id":"T7100","span":{"begin":848,"end":855},"obj":"Positive_regulation"},{"id":"T7101","span":{"begin":868,"end":880},"obj":"Positive_regulation"},{"id":"T7102","span":{"begin":884,"end":889},"obj":"Protein"},{"id":"T7103","span":{"begin":990,"end":997},"obj":"Transcription"},{"id":"T7104","span":{"begin":998,"end":1003},"obj":"Protein"},{"id":"T7105","span":{"begin":1098,"end":1103},"obj":"Protein"},{"id":"T7106","span":{"begin":1217,"end":1222},"obj":"Protein"},{"id":"T7107","span":{"begin":1243,"end":1252},"obj":"Binding"},{"id":"T7108","span":{"begin":1269,"end":1274},"obj":"Protein"}],"relations":[{"id":"R5565","pred":"themeOf","subj":"T7087","obj":"T7088"},{"id":"R5566","pred":"themeOf","subj":"T7091","obj":"T7090"},{"id":"R5567","pred":"themeOf","subj":"T7093","obj":"T7092"},{"id":"R5568","pred":"themeOf","subj":"T7096","obj":"T7095"},{"id":"R5569","pred":"themeOf","subj":"T7096","obj":"T7097"},{"id":"R5570","pred":"themeOf","subj":"T7098","obj":"T7099"},{"id":"R5571","pred":"themeOf","subj":"T7101","obj":"T7100"},{"id":"R5572","pred":"themeOf","subj":"T7102","obj":"T7101"},{"id":"R5573","pred":"themeOf","subj":"T7104","obj":"T7103"},{"id":"R5574","pred":"themeOf","subj":"T7108","obj":"T7107"}],"attributes":[{"id":"M97","pred":"Speculation","subj":"T7090","obj":"true"},{"id":"M98","pred":"Negation","subj":"T7103","obj":"true"},{"id":"M99","pred":"Negation","subj":"T7107","obj":"true"}],"text":"BMP-6 production in B cells\nThe fact that BMP-6 has been reported to act as an autocrine stimulator in chondrocytes [18] and ovarium [19], prompted us to investigate whether normal human B cells could produce BMP-6 upon stimulation. Ramos cells, which have been described to express BMP-6 mRNA endogenously [20], and the T cell line Jurkat, served as positive and negative controls, respectively. Endogenous BMP-6 mRNA levels in normal B cells were quantified by real-time RT-PCR after stimulation with anti-IgM for different time points. Interestingly, the up-regulation of BMP-6 mRNA was characteristic of an early-to intermediate inducible gene with maximal upregulation four hours after the addition of anti-IgM. The level of BMP-6 mRNA was back to baseline after 24 hours upon stimulation (Figure 10). Furthermore, both FCS and human AB-serum induced significant upregulation of BMP-6 mRNA (Figure 11). Interestingly, in a separate study we have found that normal human T cells do not express BMP-6 mRNA after activation (Sivertsen et al, manuscript in preparation). Next, we wanted to detect BMP-6 protein in normal B-cells and tested various commercially available antibodies. However, in our hands these anti-BMP-6 antibodies did only recognize the recombinant BMP-6 protein and not the native protein."}
bionlp-st-ge-2016-reference-tees
{"project":"bionlp-st-ge-2016-reference-tees","denotations":[{"id":"T7031","span":{"begin":42,"end":47},"obj":"Protein"},{"id":"T7032","span":{"begin":201,"end":208},"obj":"Gene_expression"},{"id":"T7033","span":{"begin":209,"end":214},"obj":"Protein"},{"id":"T7034","span":{"begin":397,"end":418},"obj":"Protein"},{"id":"T7035","span":{"begin":503,"end":511},"obj":"Protein"},{"id":"T7036","span":{"begin":558,"end":571},"obj":"Positive_regulation"},{"id":"T7037","span":{"begin":575,"end":585},"obj":"Protein"},{"id":"T7038","span":{"begin":707,"end":715},"obj":"Protein"},{"id":"T7039","span":{"begin":730,"end":740},"obj":"Protein"},{"id":"T7040","span":{"begin":1098,"end":1111},"obj":"Protein"},{"id":"T7041","span":{"begin":1243,"end":1252},"obj":"Binding"},{"id":"T7042","span":{"begin":1257,"end":1282},"obj":"Protein"}],"relations":[{"id":"R5542","pred":"themeOf","subj":"T7033","obj":"T7032"},{"id":"R5543","pred":"themeOf","subj":"T7037","obj":"T7036"},{"id":"R5544","pred":"themeOf","subj":"T7042","obj":"T7041"}],"text":"BMP-6 production in B cells\nThe fact that BMP-6 has been reported to act as an autocrine stimulator in chondrocytes [18] and ovarium [19], prompted us to investigate whether normal human B cells could produce BMP-6 upon stimulation. Ramos cells, which have been described to express BMP-6 mRNA endogenously [20], and the T cell line Jurkat, served as positive and negative controls, respectively. Endogenous BMP-6 mRNA levels in normal B cells were quantified by real-time RT-PCR after stimulation with anti-IgM for different time points. Interestingly, the up-regulation of BMP-6 mRNA was characteristic of an early-to intermediate inducible gene with maximal upregulation four hours after the addition of anti-IgM. The level of BMP-6 mRNA was back to baseline after 24 hours upon stimulation (Figure 10). Furthermore, both FCS and human AB-serum induced significant upregulation of BMP-6 mRNA (Figure 11). Interestingly, in a separate study we have found that normal human T cells do not express BMP-6 mRNA after activation (Sivertsen et al, manuscript in preparation). Next, we wanted to detect BMP-6 protein in normal B-cells and tested various commercially available antibodies. However, in our hands these anti-BMP-6 antibodies did only recognize the recombinant BMP-6 protein and not the native protein."}
bionlp-st-ge-2016-reference
{"project":"bionlp-st-ge-2016-reference","denotations":[{"id":"T6459","span":{"begin":0,"end":5},"obj":"Protein"},{"id":"T6460","span":{"begin":6,"end":16},"obj":"Gene_expression"},{"id":"T6461","span":{"begin":42,"end":47},"obj":"Protein"},{"id":"T6462","span":{"begin":201,"end":208},"obj":"Gene_expression"},{"id":"T6463","span":{"begin":209,"end":214},"obj":"Protein"},{"id":"T6464","span":{"begin":275,"end":282},"obj":"Transcription"},{"id":"T6465","span":{"begin":283,"end":288},"obj":"Protein"},{"id":"T6466","span":{"begin":408,"end":413},"obj":"Protein"},{"id":"T6467","span":{"begin":558,"end":571},"obj":"Positive_regulation"},{"id":"T6468","span":{"begin":575,"end":580},"obj":"Protein"},{"id":"T6469","span":{"begin":661,"end":673},"obj":"Positive_regulation"},{"id":"T6470","span":{"begin":730,"end":735},"obj":"Protein"},{"id":"T6471","span":{"begin":745,"end":761},"obj":"Negative_regulation"},{"id":"T6472","span":{"begin":848,"end":855},"obj":"Positive_regulation"},{"id":"T6473","span":{"begin":868,"end":880},"obj":"Positive_regulation"},{"id":"T6474","span":{"begin":884,"end":889},"obj":"Protein"},{"id":"T6475","span":{"begin":990,"end":997},"obj":"Transcription"},{"id":"T6476","span":{"begin":998,"end":1003},"obj":"Protein"},{"id":"T6477","span":{"begin":1098,"end":1103},"obj":"Protein"},{"id":"T6478","span":{"begin":1217,"end":1222},"obj":"Protein"},{"id":"T6479","span":{"begin":1243,"end":1252},"obj":"Binding"},{"id":"T6480","span":{"begin":1269,"end":1274},"obj":"Protein"}],"relations":[{"id":"R5071","pred":"themeOf","subj":"T6459","obj":"T6460"},{"id":"R5072","pred":"themeOf","subj":"T6463","obj":"T6462"},{"id":"R5073","pred":"themeOf","subj":"T6465","obj":"T6464"},{"id":"R5074","pred":"themeOf","subj":"T6468","obj":"T6467"},{"id":"R5075","pred":"themeOf","subj":"T6468","obj":"T6469"},{"id":"R5076","pred":"themeOf","subj":"T6470","obj":"T6471"},{"id":"R5077","pred":"themeOf","subj":"T6473","obj":"T6472"},{"id":"R5078","pred":"themeOf","subj":"T6474","obj":"T6473"},{"id":"R5079","pred":"themeOf","subj":"T6476","obj":"T6475"},{"id":"R5080","pred":"themeOf","subj":"T6480","obj":"T6479"}],"attributes":[{"id":"M90","pred":"Negation","subj":"T6479","obj":"true"},{"id":"M89","pred":"Negation","subj":"T6475","obj":"true"},{"id":"M88","pred":"Speculation","subj":"T6462","obj":"true"}],"namespaces":[{"prefix":"_base","uri":"http://bionlp.dbcls.jp/ontology/ge.owl#"}],"text":"BMP-6 production in B cells\nThe fact that BMP-6 has been reported to act as an autocrine stimulator in chondrocytes [18] and ovarium [19], prompted us to investigate whether normal human B cells could produce BMP-6 upon stimulation. Ramos cells, which have been described to express BMP-6 mRNA endogenously [20], and the T cell line Jurkat, served as positive and negative controls, respectively. Endogenous BMP-6 mRNA levels in normal B cells were quantified by real-time RT-PCR after stimulation with anti-IgM for different time points. Interestingly, the up-regulation of BMP-6 mRNA was characteristic of an early-to intermediate inducible gene with maximal upregulation four hours after the addition of anti-IgM. The level of BMP-6 mRNA was back to baseline after 24 hours upon stimulation (Figure 10). Furthermore, both FCS and human AB-serum induced significant upregulation of BMP-6 mRNA (Figure 11). Interestingly, in a separate study we have found that normal human T cells do not express BMP-6 mRNA after activation (Sivertsen et al, manuscript in preparation). Next, we wanted to detect BMP-6 protein in normal B-cells and tested various commercially available antibodies. However, in our hands these anti-BMP-6 antibodies did only recognize the recombinant BMP-6 protein and not the native protein."}
bionlp-st-ge-2016-uniprot
{"project":"bionlp-st-ge-2016-uniprot","denotations":[{"id":"T6717","span":{"begin":0,"end":3},"obj":"P22004"},{"id":"T6718","span":{"begin":0,"end":3},"obj":"P18075"},{"id":"T6719","span":{"begin":0,"end":3},"obj":"P12644"},{"id":"T6720","span":{"begin":0,"end":3},"obj":"P12643"},{"id":"T6721","span":{"begin":0,"end":5},"obj":"P22004"},{"id":"T6722","span":{"begin":42,"end":45},"obj":"P18075"},{"id":"T6723","span":{"begin":42,"end":45},"obj":"P12644"},{"id":"T6724","span":{"begin":42,"end":45},"obj":"P12643"},{"id":"T6725","span":{"begin":42,"end":45},"obj":"P22004"},{"id":"T6726","span":{"begin":42,"end":47},"obj":"P22004"},{"id":"T6727","span":{"begin":209,"end":212},"obj":"P12644"},{"id":"T6728","span":{"begin":209,"end":212},"obj":"P12643"},{"id":"T6729","span":{"begin":209,"end":212},"obj":"P18075"},{"id":"T6730","span":{"begin":209,"end":212},"obj":"P22004"},{"id":"T6731","span":{"begin":209,"end":214},"obj":"P22004"},{"id":"T6732","span":{"begin":283,"end":286},"obj":"P18075"},{"id":"T6733","span":{"begin":283,"end":286},"obj":"P12643"},{"id":"T6734","span":{"begin":283,"end":286},"obj":"P12644"},{"id":"T6735","span":{"begin":283,"end":286},"obj":"P22004"},{"id":"T6736","span":{"begin":283,"end":288},"obj":"P22004"},{"id":"T6737","span":{"begin":408,"end":411},"obj":"P12644"},{"id":"T6738","span":{"begin":408,"end":411},"obj":"P18075"},{"id":"T6739","span":{"begin":408,"end":411},"obj":"P22004"},{"id":"T6740","span":{"begin":408,"end":411},"obj":"P12643"},{"id":"T6741","span":{"begin":408,"end":413},"obj":"P22004"},{"id":"T6742","span":{"begin":575,"end":578},"obj":"P22004"},{"id":"T6743","span":{"begin":575,"end":578},"obj":"P12643"},{"id":"T6744","span":{"begin":575,"end":578},"obj":"P12644"},{"id":"T6745","span":{"begin":575,"end":578},"obj":"P18075"},{"id":"T6746","span":{"begin":575,"end":580},"obj":"P22004"},{"id":"T6747","span":{"begin":730,"end":733},"obj":"P18075"},{"id":"T6748","span":{"begin":730,"end":733},"obj":"P12643"},{"id":"T6749","span":{"begin":730,"end":733},"obj":"P12644"},{"id":"T6750","span":{"begin":730,"end":733},"obj":"P22004"},{"id":"T6751","span":{"begin":730,"end":735},"obj":"P22004"},{"id":"T6752","span":{"begin":884,"end":887},"obj":"P22004"},{"id":"T6753","span":{"begin":884,"end":887},"obj":"P18075"},{"id":"T6754","span":{"begin":884,"end":887},"obj":"P12644"},{"id":"T6755","span":{"begin":884,"end":887},"obj":"P12643"},{"id":"T6756","span":{"begin":884,"end":889},"obj":"P22004"},{"id":"T6757","span":{"begin":998,"end":1001},"obj":"P18075"},{"id":"T6758","span":{"begin":998,"end":1001},"obj":"P12644"},{"id":"T6759","span":{"begin":998,"end":1001},"obj":"P12643"},{"id":"T6760","span":{"begin":998,"end":1001},"obj":"P22004"},{"id":"T6761","span":{"begin":998,"end":1003},"obj":"P22004"},{"id":"T6762","span":{"begin":1098,"end":1101},"obj":"P12643"},{"id":"T6763","span":{"begin":1098,"end":1101},"obj":"P22004"},{"id":"T6764","span":{"begin":1098,"end":1101},"obj":"P12644"},{"id":"T6765","span":{"begin":1098,"end":1101},"obj":"P18075"},{"id":"T6766","span":{"begin":1098,"end":1103},"obj":"P22004"},{"id":"T6767","span":{"begin":1217,"end":1220},"obj":"P12644"},{"id":"T6768","span":{"begin":1217,"end":1220},"obj":"P18075"},{"id":"T6769","span":{"begin":1217,"end":1220},"obj":"P12643"},{"id":"T6770","span":{"begin":1217,"end":1220},"obj":"P22004"},{"id":"T6771","span":{"begin":1217,"end":1222},"obj":"P22004"},{"id":"T6772","span":{"begin":1269,"end":1272},"obj":"P12644"},{"id":"T6773","span":{"begin":1269,"end":1272},"obj":"P22004"},{"id":"T6774","span":{"begin":1269,"end":1272},"obj":"P18075"},{"id":"T6775","span":{"begin":1269,"end":1272},"obj":"P12643"},{"id":"T6776","span":{"begin":1269,"end":1274},"obj":"P22004"}],"namespaces":[{"prefix":"_base","uri":"http://www.uniprot.org/uniprot/"}],"text":"BMP-6 production in B cells\nThe fact that BMP-6 has been reported to act as an autocrine stimulator in chondrocytes [18] and ovarium [19], prompted us to investigate whether normal human B cells could produce BMP-6 upon stimulation. Ramos cells, which have been described to express BMP-6 mRNA endogenously [20], and the T cell line Jurkat, served as positive and negative controls, respectively. Endogenous BMP-6 mRNA levels in normal B cells were quantified by real-time RT-PCR after stimulation with anti-IgM for different time points. Interestingly, the up-regulation of BMP-6 mRNA was characteristic of an early-to intermediate inducible gene with maximal upregulation four hours after the addition of anti-IgM. The level of BMP-6 mRNA was back to baseline after 24 hours upon stimulation (Figure 10). Furthermore, both FCS and human AB-serum induced significant upregulation of BMP-6 mRNA (Figure 11). Interestingly, in a separate study we have found that normal human T cells do not express BMP-6 mRNA after activation (Sivertsen et al, manuscript in preparation). Next, we wanted to detect BMP-6 protein in normal B-cells and tested various commercially available antibodies. However, in our hands these anti-BMP-6 antibodies did only recognize the recombinant BMP-6 protein and not the native protein."}
test2
{"project":"test2","denotations":[{"id":"T6436","span":{"begin":0,"end":5},"obj":"Protein"},{"id":"T6437","span":{"begin":6,"end":16},"obj":"Gene_expression"},{"id":"T6438","span":{"begin":42,"end":47},"obj":"Protein"},{"id":"T6439","span":{"begin":201,"end":208},"obj":"Gene_expression"},{"id":"T6440","span":{"begin":209,"end":214},"obj":"Protein"},{"id":"T6441","span":{"begin":275,"end":282},"obj":"Gene_expression"},{"id":"T6442","span":{"begin":283,"end":288},"obj":"Protein"},{"id":"T6443","span":{"begin":408,"end":413},"obj":"Protein"},{"id":"T6444","span":{"begin":419,"end":425},"obj":"Transcription"},{"id":"T6445","span":{"begin":558,"end":571},"obj":"Positive_regulation"},{"id":"T6446","span":{"begin":575,"end":580},"obj":"Protein"},{"id":"T6447","span":{"begin":730,"end":735},"obj":"Protein"},{"id":"T6448","span":{"begin":745,"end":752},"obj":"Positive_regulation"},{"id":"T6449","span":{"begin":753,"end":761},"obj":"Negative_regulation"},{"id":"T6450","span":{"begin":848,"end":855},"obj":"Positive_regulation"},{"id":"T6451","span":{"begin":868,"end":880},"obj":"Positive_regulation"},{"id":"T6452","span":{"begin":884,"end":889},"obj":"Protein"},{"id":"T6453","span":{"begin":990,"end":997},"obj":"Gene_expression"},{"id":"T6454","span":{"begin":998,"end":1003},"obj":"Protein"},{"id":"T6455","span":{"begin":1098,"end":1103},"obj":"Protein"},{"id":"T6456","span":{"begin":1217,"end":1222},"obj":"Protein"},{"id":"T6457","span":{"begin":1269,"end":1274},"obj":"Protein"}],"relations":[{"id":"R5063","pred":"themeOf","subj":"T6436","obj":"T6437"},{"id":"R5064","pred":"themeOf","subj":"T6440","obj":"T6439"},{"id":"R5065","pred":"themeOf","subj":"T6442","obj":"T6441"},{"id":"R5066","pred":"themeOf","subj":"T6443","obj":"T6444"},{"id":"R5067","pred":"themeOf","subj":"T6446","obj":"T6445"},{"id":"R5068","pred":"themeOf","subj":"T6451","obj":"T6450"},{"id":"R5069","pred":"themeOf","subj":"T6452","obj":"T6451"},{"id":"R5070","pred":"themeOf","subj":"T6454","obj":"T6453"}],"attributes":[{"id":"M87","pred":"Negation","subj":"T6445","obj":"true"},{"id":"M85","pred":"Speculation","subj":"T6437","obj":"true"},{"id":"M86","pred":"Negation","subj":"T6444","obj":"true"}],"text":"BMP-6 production in B cells\nThe fact that BMP-6 has been reported to act as an autocrine stimulator in chondrocytes [18] and ovarium [19], prompted us to investigate whether normal human B cells could produce BMP-6 upon stimulation. Ramos cells, which have been described to express BMP-6 mRNA endogenously [20], and the T cell line Jurkat, served as positive and negative controls, respectively. Endogenous BMP-6 mRNA levels in normal B cells were quantified by real-time RT-PCR after stimulation with anti-IgM for different time points. Interestingly, the up-regulation of BMP-6 mRNA was characteristic of an early-to intermediate inducible gene with maximal upregulation four hours after the addition of anti-IgM. The level of BMP-6 mRNA was back to baseline after 24 hours upon stimulation (Figure 10). Furthermore, both FCS and human AB-serum induced significant upregulation of BMP-6 mRNA (Figure 11). Interestingly, in a separate study we have found that normal human T cells do not express BMP-6 mRNA after activation (Sivertsen et al, manuscript in preparation). Next, we wanted to detect BMP-6 protein in normal B-cells and tested various commercially available antibodies. However, in our hands these anti-BMP-6 antibodies did only recognize the recombinant BMP-6 protein and not the native protein."}
tees-test
{"project":"tees-test","denotations":[{"id":"T1196","span":{"begin":0,"end":5},"obj":"D055418"},{"id":"T1197","span":{"begin":0,"end":5},"obj":"D055418"},{"id":"T1198","span":{"begin":0,"end":5},"obj":"Q04906"},{"id":"T1199","span":{"begin":0,"end":5},"obj":"PR:000000167"},{"id":"T1200","span":{"begin":0,"end":5},"obj":"P20722"},{"id":"T1201","span":{"begin":0,"end":5},"obj":"PR:Q04906"},{"id":"T1202","span":{"begin":0,"end":5},"obj":"P22004"},{"id":"T1203","span":{"begin":0,"end":5},"obj":"PR:P22004"},{"id":"T1204","span":{"begin":0,"end":5},"obj":"PR:P20722"},{"id":"T1205","span":{"begin":42,"end":47},"obj":"D055418"},{"id":"T1206","span":{"begin":42,"end":47},"obj":"D055418"},{"id":"T1207","span":{"begin":42,"end":47},"obj":"Q04906"},{"id":"T1208","span":{"begin":42,"end":47},"obj":"PR:000000167"},{"id":"T1209","span":{"begin":42,"end":47},"obj":"P20722"},{"id":"T1210","span":{"begin":42,"end":47},"obj":"PR:Q04906"},{"id":"T1211","span":{"begin":42,"end":47},"obj":"P22004"},{"id":"T1212","span":{"begin":42,"end":47},"obj":"PR:P22004"},{"id":"T1213","span":{"begin":42,"end":47},"obj":"PR:P20722"},{"id":"T1214","span":{"begin":125,"end":132},"obj":"UBERON:0000992"},{"id":"T1215","span":{"begin":181,"end":186},"obj":"D006801"},{"id":"T1216","span":{"begin":209,"end":214},"obj":"D055418"},{"id":"T1217","span":{"begin":209,"end":214},"obj":"D055418"},{"id":"T1218","span":{"begin":209,"end":214},"obj":"Q04906"},{"id":"T1219","span":{"begin":209,"end":214},"obj":"PR:000000167"},{"id":"T1220","span":{"begin":209,"end":214},"obj":"P20722"},{"id":"T1221","span":{"begin":209,"end":214},"obj":"PR:Q04906"},{"id":"T1222","span":{"begin":209,"end":214},"obj":"P22004"},{"id":"T1223","span":{"begin":209,"end":214},"obj":"PR:P22004"},{"id":"T1224","span":{"begin":209,"end":214},"obj":"PR:P20722"},{"id":"T1225","span":{"begin":233,"end":238},"obj":"CVCL_0597"},{"id":"T1226","span":{"begin":283,"end":288},"obj":"D055418"},{"id":"T1227","span":{"begin":283,"end":288},"obj":"D055418"},{"id":"T1228","span":{"begin":283,"end":288},"obj":"Q04906"},{"id":"T1229","span":{"begin":283,"end":288},"obj":"PR:000000167"},{"id":"T1230","span":{"begin":283,"end":288},"obj":"P20722"},{"id":"T1231","span":{"begin":283,"end":288},"obj":"PR:Q04906"},{"id":"T1232","span":{"begin":283,"end":288},"obj":"P22004"},{"id":"T1233","span":{"begin":283,"end":288},"obj":"PR:P22004"},{"id":"T1234","span":{"begin":283,"end":288},"obj":"PR:P20722"},{"id":"T1235","span":{"begin":289,"end":293},"obj":"D012333"},{"id":"T1236","span":{"begin":289,"end":293},"obj":"CHEBI:33699"},{"id":"T1237","span":{"begin":289,"end":293},"obj":"SO:0000234"},{"id":"T1238","span":{"begin":321,"end":327},"obj":"CL:0000084"},{"id":"T1239","span":{"begin":333,"end":339},"obj":"CVCL_0065"},{"id":"T1240","span":{"begin":408,"end":413},"obj":"D055418"},{"id":"T1241","span":{"begin":408,"end":413},"obj":"D055418"},{"id":"T1242","span":{"begin":408,"end":413},"obj":"Q04906"},{"id":"T1243","span":{"begin":408,"end":413},"obj":"PR:000000167"},{"id":"T1244","span":{"begin":408,"end":413},"obj":"P20722"},{"id":"T1245","span":{"begin":408,"end":413},"obj":"PR:Q04906"},{"id":"T1246","span":{"begin":408,"end":413},"obj":"P22004"},{"id":"T1247","span":{"begin":408,"end":413},"obj":"PR:P22004"},{"id":"T1248","span":{"begin":408,"end":413},"obj":"PR:P20722"},{"id":"T1249","span":{"begin":414,"end":418},"obj":"D012333"},{"id":"T1250","span":{"begin":414,"end":418},"obj":"CHEBI:33699"},{"id":"T1251","span":{"begin":414,"end":418},"obj":"SO:0000234"},{"id":"T1252","span":{"begin":476,"end":479},"obj":"Q41249"},{"id":"T1253","span":{"begin":476,"end":479},"obj":"O66148"},{"id":"T1254","span":{"begin":476,"end":479},"obj":"Q59987"},{"id":"T1255","span":{"begin":476,"end":479},"obj":"Q01289"},{"id":"T1256","span":{"begin":476,"end":479},"obj":"O80333"},{"id":"T1257","span":{"begin":476,"end":479},"obj":"P15904"},{"id":"T1258","span":{"begin":476,"end":479},"obj":"Q9SDT1"},{"id":"T1259","span":{"begin":476,"end":479},"obj":"Q39617"},{"id":"T1260","span":{"begin":503,"end":511},"obj":"C044203"},{"id":"T1261","span":{"begin":561,"end":571},"obj":"GO:0065007"},{"id":"T1262","span":{"begin":575,"end":580},"obj":"D055418"},{"id":"T1263","span":{"begin":575,"end":580},"obj":"D055418"},{"id":"T1264","span":{"begin":575,"end":580},"obj":"Q04906"},{"id":"T1265","span":{"begin":575,"end":580},"obj":"PR:000000167"},{"id":"T1266","span":{"begin":575,"end":580},"obj":"P20722"},{"id":"T1267","span":{"begin":575,"end":580},"obj":"PR:Q04906"},{"id":"T1268","span":{"begin":575,"end":580},"obj":"P22004"},{"id":"T1269","span":{"begin":575,"end":580},"obj":"PR:P22004"},{"id":"T1270","span":{"begin":575,"end":580},"obj":"PR:P20722"},{"id":"T1271","span":{"begin":581,"end":585},"obj":"D012333"},{"id":"T1272","span":{"begin":581,"end":585},"obj":"CHEBI:33699"},{"id":"T1273","span":{"begin":581,"end":585},"obj":"SO:0000234"},{"id":"T1274","span":{"begin":620,"end":632},"obj":"SO:0000933"},{"id":"T1275","span":{"begin":643,"end":647},"obj":"SO:0000704"},{"id":"T1276","span":{"begin":707,"end":715},"obj":"C044203"},{"id":"T1277","span":{"begin":730,"end":735},"obj":"D055418"},{"id":"T1278","span":{"begin":730,"end":735},"obj":"D055418"},{"id":"T1279","span":{"begin":730,"end":735},"obj":"Q04906"},{"id":"T1280","span":{"begin":730,"end":735},"obj":"PR:000000167"},{"id":"T1281","span":{"begin":730,"end":735},"obj":"P20722"},{"id":"T1282","span":{"begin":730,"end":735},"obj":"PR:Q04906"},{"id":"T1283","span":{"begin":730,"end":735},"obj":"P22004"},{"id":"T1284","span":{"begin":730,"end":735},"obj":"PR:P22004"},{"id":"T1285","span":{"begin":730,"end":735},"obj":"PR:P20722"},{"id":"T1286","span":{"begin":736,"end":740},"obj":"D012333"},{"id":"T1287","span":{"begin":736,"end":740},"obj":"CHEBI:33699"},{"id":"T1288","span":{"begin":736,"end":740},"obj":"SO:0000234"},{"id":"T1289","span":{"begin":833,"end":838},"obj":"D006801"},{"id":"T1290","span":{"begin":842,"end":847},"obj":"UBERON:0001977"},{"id":"T1291","span":{"begin":884,"end":889},"obj":"D055418"},{"id":"T1292","span":{"begin":884,"end":889},"obj":"D055418"},{"id":"T1293","span":{"begin":884,"end":889},"obj":"Q04906"},{"id":"T1294","span":{"begin":884,"end":889},"obj":"PR:000000167"},{"id":"T1295","span":{"begin":884,"end":889},"obj":"P20722"},{"id":"T1296","span":{"begin":884,"end":889},"obj":"PR:Q04906"},{"id":"T1297","span":{"begin":884,"end":889},"obj":"P22004"},{"id":"T1298","span":{"begin":884,"end":889},"obj":"PR:P22004"},{"id":"T1299","span":{"begin":884,"end":889},"obj":"PR:P20722"},{"id":"T1300","span":{"begin":890,"end":894},"obj":"D012333"},{"id":"T1301","span":{"begin":890,"end":894},"obj":"CHEBI:33699"},{"id":"T1302","span":{"begin":890,"end":894},"obj":"SO:0000234"},{"id":"T1303","span":{"begin":969,"end":974},"obj":"D006801"},{"id":"T1304","span":{"begin":998,"end":1003},"obj":"D055418"},{"id":"T1305","span":{"begin":998,"end":1003},"obj":"D055418"},{"id":"T1306","span":{"begin":998,"end":1003},"obj":"Q04906"},{"id":"T1307","span":{"begin":998,"end":1003},"obj":"PR:000000167"},{"id":"T1308","span":{"begin":998,"end":1003},"obj":"P20722"},{"id":"T1309","span":{"begin":998,"end":1003},"obj":"PR:Q04906"},{"id":"T1310","span":{"begin":998,"end":1003},"obj":"P22004"},{"id":"T1311","span":{"begin":998,"end":1003},"obj":"PR:P22004"},{"id":"T1312","span":{"begin":998,"end":1003},"obj":"PR:P20722"},{"id":"T1313","span":{"begin":1004,"end":1008},"obj":"D012333"},{"id":"T1314","span":{"begin":1004,"end":1008},"obj":"CHEBI:33699"},{"id":"T1315","span":{"begin":1004,"end":1008},"obj":"SO:0000234"},{"id":"T1316","span":{"begin":1098,"end":1103},"obj":"D055418"},{"id":"T1317","span":{"begin":1098,"end":1103},"obj":"D055418"},{"id":"T1318","span":{"begin":1098,"end":1103},"obj":"Q04906"},{"id":"T1319","span":{"begin":1098,"end":1103},"obj":"PR:000000167"},{"id":"T1320","span":{"begin":1098,"end":1103},"obj":"P20722"},{"id":"T1321","span":{"begin":1098,"end":1103},"obj":"PR:Q04906"},{"id":"T1322","span":{"begin":1098,"end":1103},"obj":"P22004"},{"id":"T1323","span":{"begin":1098,"end":1103},"obj":"PR:P22004"},{"id":"T1324","span":{"begin":1098,"end":1103},"obj":"PR:P20722"},{"id":"T1325","span":{"begin":1104,"end":1111},"obj":"CHEBI:36080"},{"id":"T1326","span":{"begin":1104,"end":1111},"obj":"CHEBI:11122"},{"id":"T1327","span":{"begin":1104,"end":1111},"obj":"PR:000000001"},{"id":"T1328","span":{"begin":1104,"end":1111},"obj":"GO:0003675"},{"id":"T1329","span":{"begin":1104,"end":1111},"obj":"SO:0000104"},{"id":"T1330","span":{"begin":1172,"end":1182},"obj":"D000906"},{"id":"T1331","span":{"begin":1217,"end":1222},"obj":"D055418"},{"id":"T1332","span":{"begin":1217,"end":1222},"obj":"D055418"},{"id":"T1333","span":{"begin":1217,"end":1222},"obj":"Q04906"},{"id":"T1334","span":{"begin":1217,"end":1222},"obj":"PR:000000167"},{"id":"T1335","span":{"begin":1217,"end":1222},"obj":"P20722"},{"id":"T1336","span":{"begin":1217,"end":1222},"obj":"PR:Q04906"},{"id":"T1337","span":{"begin":1217,"end":1222},"obj":"P22004"},{"id":"T1338","span":{"begin":1217,"end":1222},"obj":"PR:P22004"},{"id":"T1339","span":{"begin":1217,"end":1222},"obj":"PR:P20722"},{"id":"T1340","span":{"begin":1223,"end":1233},"obj":"D000906"},{"id":"T1341","span":{"begin":1269,"end":1274},"obj":"D055418"},{"id":"T1342","span":{"begin":1269,"end":1274},"obj":"D055418"},{"id":"T1343","span":{"begin":1269,"end":1274},"obj":"Q04906"},{"id":"T1344","span":{"begin":1269,"end":1274},"obj":"PR:000000167"},{"id":"T1345","span":{"begin":1269,"end":1274},"obj":"P20722"},{"id":"T1346","span":{"begin":1269,"end":1274},"obj":"PR:Q04906"},{"id":"T1347","span":{"begin":1269,"end":1274},"obj":"P22004"},{"id":"T1348","span":{"begin":1269,"end":1274},"obj":"PR:P22004"},{"id":"T1349","span":{"begin":1269,"end":1274},"obj":"PR:P20722"},{"id":"T1350","span":{"begin":1275,"end":1282},"obj":"CHEBI:36080"},{"id":"T1351","span":{"begin":1275,"end":1282},"obj":"CHEBI:11122"},{"id":"T1352","span":{"begin":1275,"end":1282},"obj":"PR:000000001"},{"id":"T1353","span":{"begin":1275,"end":1282},"obj":"GO:0003675"},{"id":"T1354","span":{"begin":1275,"end":1282},"obj":"SO:0000104"},{"id":"T1355","span":{"begin":1302,"end":1309},"obj":"CHEBI:36080"},{"id":"T1356","span":{"begin":1302,"end":1309},"obj":"CHEBI:11122"},{"id":"T1357","span":{"begin":1302,"end":1309},"obj":"PR:000000001"},{"id":"T1358","span":{"begin":1302,"end":1309},"obj":"GO:0003675"},{"id":"T1359","span":{"begin":1302,"end":1309},"obj":"SO:0000104"}],"text":"BMP-6 production in B cells\nThe fact that BMP-6 has been reported to act as an autocrine stimulator in chondrocytes [18] and ovarium [19], prompted us to investigate whether normal human B cells could produce BMP-6 upon stimulation. Ramos cells, which have been described to express BMP-6 mRNA endogenously [20], and the T cell line Jurkat, served as positive and negative controls, respectively. Endogenous BMP-6 mRNA levels in normal B cells were quantified by real-time RT-PCR after stimulation with anti-IgM for different time points. Interestingly, the up-regulation of BMP-6 mRNA was characteristic of an early-to intermediate inducible gene with maximal upregulation four hours after the addition of anti-IgM. The level of BMP-6 mRNA was back to baseline after 24 hours upon stimulation (Figure 10). Furthermore, both FCS and human AB-serum induced significant upregulation of BMP-6 mRNA (Figure 11). Interestingly, in a separate study we have found that normal human T cells do not express BMP-6 mRNA after activation (Sivertsen et al, manuscript in preparation). Next, we wanted to detect BMP-6 protein in normal B-cells and tested various commercially available antibodies. However, in our hands these anti-BMP-6 antibodies did only recognize the recombinant BMP-6 protein and not the native protein."}