PMC:1129089 / 21259-22920 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/1129089","sourcedb":"PMC","sourceid":"1129089","source_url":"https://www.ncbi.nlm.nih.gov/pmc/1129089","text":"MagT1 expression is responsive to magnesium\nThe rationale for these studies is based on the observation that renal magnesium conservation is principally regulated by differential expression of genes encoding magnesium transport proteins. Accordingly, we determined the response of MagT1 to changes in magnesium at the messenger and protein levels. These studies were performed with distal tubule epithelial cells, MDCT, cultured in media containing normal (1.0 mM) or low (nominally magnesium-free) magnesium concentrations for 16 h and on kidney cortex tissue harvested from mice maintained on either normal or magnesium-restricted diets for 5 days. The mRNA and protein expression was relatively stronger in cells cultured in low magnesium media and in tissue of mice maintained on low magnesium diets (urine and plasma magnesium concentration, 1.1 ± 0.3 and 0.13 ± 0.01 mM, respectively) compared to normal cells and tissue of animals on normal diets (urine and plasma magnesium, 13.2 ± 1.2 and 0.75 ± 0.09 mM, respectively). MDCT and tissue mMagT1 mRNA, as measured by real-time RT-PCR was increased by 2.1-fold and 2.3-fold, respectively (Figure 12). In association with the increases in mRNA, MagT1 protein was increased by 31 ± 12% in the cultured epithelial cells and 33 ± 6 % in kidney cortex with low magnesium relative to the respective controls (Figure 13). Accordingly, it is apparent that an increase in mRNA levels is translated into higher protein expression and by inference leads to greater magnesium transport (the latter conclusion is based on the urinary magnesium excretion of animals maintained on low magnesium relative to normal diets).","divisions":[{"label":"Title","span":{"begin":0,"end":43}}],"tracks":[]}