PMC:1087847 / 38374-38991 JSONTXT

Annnotations TAB JSON ListView MergeView

    craft-sa-dev

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presence of Annexin A7 mRNA in mouse ES cells and embryos E7, E11, E15 and E17 was analyzed by northern blot analysis. 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    biosemtest

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presence of Annexin A7 mRNA in mouse ES cells and embryos E7, E11, E15 and E17 was analyzed by northern blot analysis. An Embryo Multiple Tissue Northern Blot membrane was obtained from Clontech Laboratories (Heidelberg, FRG). It contained approximately 2 μg of poly(A)+ RNA per lane from four different stages of mouse development. RNA from ES cells was isolated with the RNeasy kit (Qiagen). The membranes were hybridized with a random-primed [α-32P]-dATP-labelled full length annexin A7 cDNA probe and with a β-actin specific probe for control in ExpressHyb solution according to the manufacturer's directions."}

    craft-ca-core-dev

    {"project":"craft-ca-core-dev","denotations":[{"id":"T11144","span":{"begin":16,"end":26},"obj":"PR:000004080"},{"id":"T11145","span":{"begin":35,"end":40},"obj":"NCBITaxon:10088"},{"id":"T11146","span":{"begin":41,"end":49},"obj":"CL:0002322"},{"id":"T11147","span":{"begin":54,"end":61},"obj":"UBERON:0000922"},{"id":"T11148","span":{"begin":126,"end":132},"obj":"UBERON:0000922"},{"id":"T11149","span":{"begin":142,"end":148},"obj":"UBERON:0000479"},{"id":"T11150","span":{"begin":318,"end":323},"obj":"NCBITaxon:10088"},{"id":"T11151","span":{"begin":346,"end":354},"obj":"CL:0002322"},{"id":"T11152","span":{"begin":417,"end":427},"obj":"GO:0097617"},{"id":"T11153","span":{"begin":452,"end":455},"obj":"CHEBI:37972"},{"id":"T11154","span":{"begin":483,"end":493},"obj":"PR:000004080"},{"id":"T11155","span":{"begin":516,"end":523},"obj":"PR:000003676"},{"id":"T11156","span":{"begin":565,"end":573},"obj":"CHEBI:75958"}],"text":"The presence of Annexin A7 mRNA in mouse ES cells and embryos E7, E11, E15 and E17 was analyzed by northern blot analysis. An Embryo Multiple Tissue Northern Blot membrane was obtained from Clontech Laboratories (Heidelberg, FRG). It contained approximately 2 μg of poly(A)+ RNA per lane from four different stages of mouse development. RNA from ES cells was isolated with the RNeasy kit (Qiagen). The membranes were hybridized with a random-primed [α-32P]-dATP-labelled full length annexin A7 cDNA probe and with a β-actin specific probe for control in ExpressHyb solution according to the manufacturer's directions."}