PMC:1087847 / 37457-37733 JSONTXT

Annnotations TAB JSON ListView MergeView

    craft-sa-dev

    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An equal volume of buffer 3 was added in aliquots (20 mM Hepes, pH 7.9, 1.5 mM MgCl2, 800 mM KCl, 0.2 mM EDTA, 25%v/v glycerine, 0.5 mM DTT, 0.5 mM PMSF, 1% NP40, protease inhibitor cocktail (Sigma)) and the solution was incubated while shaking for further 30 minutes on ice."}

    craft-ca-core-ex-dev

    {"project":"craft-ca-core-ex-dev","denotations":[{"id":"T10522","span":{"begin":20,"end":26},"obj":"CHEBI_CHMO_EXT:buffer_solution"},{"id":"T10523","span":{"begin":58,"end":63},"obj":"CHEBI:46756"},{"id":"T10524","span":{"begin":80,"end":85},"obj":"CHEBI:6636"},{"id":"T10525","span":{"begin":94,"end":97},"obj":"CHEBI:32588"},{"id":"T10526","span":{"begin":106,"end":110},"obj":"CHEBI_EXT:EDTA"},{"id":"T10527","span":{"begin":119,"end":128},"obj":"CHEBI:17754"},{"id":"T10528","span":{"begin":137,"end":140},"obj":"CHEBI:18320"},{"id":"T10529","span":{"begin":149,"end":153},"obj":"CHEBI:8102"},{"id":"T10530","span":{"begin":158,"end":162},"obj":"CHEBI:63016"},{"id":"T10531","span":{"begin":164,"end":182},"obj":"CHEBI_GO_EXT:peptidase_or_protease_or_proteinase_inhibitor"},{"id":"T10532","span":{"begin":183,"end":191},"obj":"CHEBI:60004"},{"id":"T10533","span":{"begin":209,"end":217},"obj":"CHEBI:75958"}],"text":" An equal volume of buffer 3 was added in aliquots (20 mM Hepes, pH 7.9, 1.5 mM MgCl2, 800 mM KCl, 0.2 mM EDTA, 25%v/v glycerine, 0.5 mM DTT, 0.5 mM PMSF, 1% NP40, protease inhibitor cocktail (Sigma)) and the solution was incubated while shaking for further 30 minutes on ice."}

    biosemtest

    {"project":"biosemtest","denotations":[{"id":"T10272","span":{"begin":68,"end":70},"obj":"UMLS/C0020283"},{"id":"T10273","span":{"begin":45,"end":53},"obj":"UMLS/C1510844"},{"id":"T10274","span":{"begin":36,"end":41},"obj":"UMLS/C1883712"},{"id":"T10277","span":{"begin":261,"end":271},"obj":"UMLS/C0456693"},{"id":"T10288","span":{"begin":167,"end":185},"obj":"UP/P80060"},{"id":"T10289","span":{"begin":13,"end":19},"obj":"UMLS/C2700258"},{"id":"T10292","span":{"begin":36,"end":41},"obj":"UMLS/C1720086"},{"id":"T10295","span":{"begin":13,"end":19},"obj":"UMLS/C1690016"},{"id":"T10299","span":{"begin":140,"end":143},"obj":"UMLS/C0012789"},{"id":"T10301","span":{"begin":7,"end":12},"obj":"UMLS/C0439089"},{"id":"T10305","span":{"begin":225,"end":234},"obj":"UMLS/C1439852"},{"id":"T10307","span":{"begin":7,"end":12},"obj":"UMLS/C0205163"},{"id":"T10312","span":{"begin":167,"end":185},"obj":"UMLS/C0033607"},{"id":"T10315","span":{"begin":109,"end":113},"obj":"UMLS/C0013618"},{"id":"T10322","span":{"begin":167,"end":185},"obj":"UP/P61153"},{"id":"T10326","span":{"begin":212,"end":220},"obj":"UMLS/C2699488"},{"id":"T10332","span":{"begin":241,"end":248},"obj":"UMLS/C1883023"},{"id":"T10340","span":{"begin":23,"end":29},"obj":"UMLS/C0006353"},{"id":"T10341","span":{"begin":212,"end":220},"obj":"UMLS/C0037633"},{"id":"T10347","span":{"begin":13,"end":19},"obj":"UMLS/C1705102"},{"id":"T10350","span":{"begin":241,"end":248},"obj":"UMLS/C0040822"},{"id":"T10363","span":{"begin":68,"end":70},"obj":"UMLS/C0450407"},{"id":"T10364","span":{"begin":212,"end":220},"obj":"UMLS/C0525069"},{"id":"T10367","span":{"begin":68,"end":70},"obj":"UMLS/C0450408"},{"id":"T10368","span":{"begin":186,"end":194},"obj":"UMLS/C0678420"},{"id":"T10370","span":{"begin":68,"end":70},"obj":"UMLS/C0450409"},{"id":"T10374","span":{"begin":68,"end":70},"obj":"UMLS/C2363150"},{"id":"T10378","span":{"begin":68,"end":70},"obj":"UMLS/C0031526"},{"id":"T10388","span":{"begin":7,"end":12},"obj":"UMLS/C1549782"},{"id":"T10393","span":{"begin":212,"end":220},"obj":"UMLS/C1382100"},{"id":"T10395","span":{"begin":196,"end":201},"obj":"UMLS/C0227391"},{"id":"T10399","span":{"begin":36,"end":41},"obj":"UMLS/C1524062"},{"id":"T10407","span":{"begin":13,"end":19},"obj":"UMLS/C0449468"},{"id":"T10414","span":{"begin":167,"end":185},"obj":"UP/P26228"},{"id":"T10418","span":{"begin":167,"end":185},"obj":"UP/P43131"},{"id":"T10423","span":{"begin":122,"end":131},"obj":"UMLS/C0017861"},{"id":"T10426","span":{"begin":109,"end":113},"obj":"UMLS/C0558745"},{"id":"T10432","span":{"begin":83,"end":88},"obj":"UMLS/C0024472"},{"id":"T10438","span":{"begin":68,"end":70},"obj":"UMLS/C1304686"},{"id":"T10443","span":{"begin":261,"end":271},"obj":"UMLS/C1442458"},{"id":"T10444","span":{"begin":196,"end":201},"obj":"UMLS/C1719918"}],"text":" An equal volume of buffer 3 was added in aliquots (20 mM Hepes, pH 7.9, 1.5 mM MgCl2, 800 mM KCl, 0.2 mM EDTA, 25%v/v glycerine, 0.5 mM DTT, 0.5 mM PMSF, 1% NP40, protease inhibitor cocktail (Sigma)) and the solution was incubated while shaking for further 30 minutes on ice."}

    craft-ca-core-dev

    {"project":"craft-ca-core-dev","denotations":[{"id":"T10478","span":{"begin":58,"end":63},"obj":"CHEBI:46756"},{"id":"T10479","span":{"begin":80,"end":85},"obj":"CHEBI:6636"},{"id":"T10480","span":{"begin":94,"end":97},"obj":"CHEBI:32588"},{"id":"T10481","span":{"begin":119,"end":128},"obj":"CHEBI:17754"},{"id":"T10482","span":{"begin":137,"end":140},"obj":"CHEBI:18320"},{"id":"T10483","span":{"begin":149,"end":153},"obj":"CHEBI:8102"},{"id":"T10484","span":{"begin":158,"end":162},"obj":"CHEBI:63016"},{"id":"T10485","span":{"begin":183,"end":191},"obj":"CHEBI:60004"},{"id":"T10486","span":{"begin":209,"end":217},"obj":"CHEBI:75958"}],"text":" An equal volume of buffer 3 was added in aliquots (20 mM Hepes, pH 7.9, 1.5 mM MgCl2, 800 mM KCl, 0.2 mM EDTA, 25%v/v glycerine, 0.5 mM DTT, 0.5 mM PMSF, 1% NP40, protease inhibitor cocktail (Sigma)) and the solution was incubated while shaking for further 30 minutes on ice."}