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{"project":"craft-ca-core-dev","denotations":[{"id":"T8926","span":{"begin":0,"end":10},"obj":"GO:0042571"},{"id":"T8927","span":{"begin":11,"end":21},"obj":"GO:0042571"},{"id":"T8928","span":{"begin":84,"end":90},"obj":"SO:0000417"},{"id":"T8929","span":{"begin":94,"end":99},"obj":"NCBITaxon:10088"},{"id":"T8930","span":{"begin":100,"end":110},"obj":"PR:000004080"},{"id":"T8931","span":{"begin":125,"end":128},"obj":"GO:0071735"},{"id":"T8932","span":{"begin":166,"end":176},"obj":"PR:000004080"},{"id":"T8933","span":{"begin":177,"end":185},"obj":"SO:0001060"},{"id":"T8934","span":{"begin":229,"end":237},"obj":"GO:0042571"},{"id":"T8935","span":{"begin":246,"end":251},"obj":"NCBITaxon:10088"},{"id":"T8936","span":{"begin":252,"end":262},"obj":"PR:000004080"},{"id":"T8937","span":{"begin":294,"end":299},"obj":"UBERON:0001977"},{"id":"T8938","span":{"begin":319,"end":328},"obj":"CL:0000127"},{"id":"T8939","span":{"begin":338,"end":359},"obj":"GO:0005882"},{"id":"T8940","span":{"begin":360,"end":365},"obj":"CL:0000125"},{"id":"T8941","span":{"begin":360,"end":391},"obj":"PR:000007939"},{"id":"T8942","span":{"begin":377,"end":383},"obj":"CHEBI:37527"},{"id":"T8943","span":{"begin":393,"end":397},"obj":"PR:000007939"},{"id":"T8944","span":{"begin":420,"end":427},"obj":"PR:000028799"},{"id":"T8945","span":{"begin":450,"end":456},"obj":"PR:000007050"},{"id":"T8946","span":{"begin":499,"end":504},"obj":"PR:000037206"}],"text":"Antibodies\nAntibodies used in this study were mAb 203–217 directed against the core domain of mouse Annexin A7 [6] (purified IgG, 1:50) specifically recognizing both Annexin A7 isoforms [[6,10], and data not shown], a polyclonal antibody against mouse Annexin A7 [10] (1:200), a polyclonal antiserum raised against the astrocyte-specific intermediate filament glial fibrillary acidic protein (GFAP; DAKO, 1:400), anti-β-tubulin (Sigma, 1:1000), anti-Emerin (novocastra, 1:2000), and polyclonal anti-LAP2α (kindly provided by Ronald Foisner, 1:5000)."}