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(A) Low magnification of the cerebellum presents an Annexin A7 expression mainly in cells of the stratum granulosum and laminae medullares. (B) Corresponding section stained for GFAP. (C) Higher magnification of folia of the cerebellum, where a polyclonal anti-AnnexinA7 antibody was used; square, a higher magnification of acorresponding area stained with mAb 203–217 is given in (D). Between stratum granulosum and stratum moleculare the layer of Purkinje-cells (stratum neuronorum piriformium (ganglionare)) can be observed. (D) Staining of the band of Purkinje-cells (arrows). The positive Annexin A7-stain in the stratum granulosum is due to staining of the nuclei of neurons. (E) In addition to an intense staining of the nucleus, the cell body is AnnexinA7-positive including both dendrites of the Purkinje-cell shown. (F) Corresponding section from an AnxA7-/- mouse. (G) AnnexinA7 staining of axons (arrowheads) running from the laminae medullares to the Purkinje-cell layer located in the round end of a convolution. Sections A, D, E, F, G were stained with mAb 203–217."}

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