Id |
Subject |
Object |
Predicate |
Lexical cue |
T261 |
8-20 |
NN |
denotes |
localization |
T262 |
0-66 |
sentence |
denotes |
Nuclear localization of Annexin A7 during murine brain development |
T263 |
21-23 |
IN |
denotes |
of |
T264 |
24-31 |
NN |
denotes |
Annexin |
T265 |
32-34 |
NN |
denotes |
A7 |
T266 |
35-41 |
IN |
denotes |
during |
T267 |
42-48 |
JJ |
denotes |
murine |
T268 |
49-54 |
NN |
denotes |
brain |
T269 |
55-66 |
NN |
denotes |
development |
T270 |
66-67 |
sentence |
denotes |
|
T271 |
77-87 |
sentence |
denotes |
Background |
T272 |
77-87 |
NN |
denotes |
Background |
T273 |
87-293 |
sentence |
denotes |
Annexin A7 is a member of the annexin protein family, which is characterized by its ability to interact with phospholipids in the presence of Ca2+-ions and which is thought to function in Ca2+-homeostasis. |
T274 |
88-95 |
NN |
denotes |
Annexin |
T275 |
96-98 |
NN |
denotes |
A7 |
T276 |
99-101 |
VBZ |
denotes |
is |
T277 |
102-103 |
DT |
denotes |
a |
T278 |
104-110 |
NN |
denotes |
member |
T279 |
111-113 |
IN |
denotes |
of |
T280 |
114-117 |
DT |
denotes |
the |
T281 |
134-140 |
NN |
denotes |
family |
T282 |
118-125 |
NN |
denotes |
annexin |
T283 |
126-133 |
NN |
denotes |
protein |
T284 |
140-142 |
, |
denotes |
, |
T285 |
142-147 |
WDT |
denotes |
which |
T286 |
151-164 |
VBN |
denotes |
characterized |
T287 |
148-150 |
VBZ |
denotes |
is |
T288 |
165-167 |
IN |
denotes |
by |
T289 |
168-171 |
PRP$ |
denotes |
its |
T290 |
172-179 |
NN |
denotes |
ability |
T291 |
180-182 |
TO |
denotes |
to |
T292 |
183-191 |
VB |
denotes |
interact |
T293 |
192-196 |
IN |
denotes |
with |
T294 |
197-210 |
NNS |
denotes |
phospholipids |
T295 |
211-213 |
IN |
denotes |
in |
T296 |
214-217 |
DT |
denotes |
the |
T297 |
218-226 |
NN |
denotes |
presence |
T298 |
227-229 |
IN |
denotes |
of |
T299 |
230-234 |
NN |
denotes |
Ca2+ |
T300 |
235-239 |
NNS |
denotes |
ions |
T301 |
234-235 |
HYPH |
denotes |
- |
T302 |
240-243 |
CC |
denotes |
and |
T303 |
244-249 |
WDT |
denotes |
which |
T304 |
253-260 |
VBN |
denotes |
thought |
T305 |
250-252 |
VBZ |
denotes |
is |
T306 |
261-263 |
TO |
denotes |
to |
T307 |
264-272 |
VB |
denotes |
function |
T308 |
273-275 |
IN |
denotes |
in |
T309 |
276-280 |
NN |
denotes |
Ca2+ |
T310 |
281-292 |
NN |
denotes |
homeostasis |
T311 |
280-281 |
HYPH |
denotes |
- |
T312 |
292-293 |
. |
denotes |
. |
T313 |
293-370 |
sentence |
denotes |
Results from mutant mice showed altered Ca2+-wave propagation in astrocytes. |
T314 |
294-301 |
NNS |
denotes |
Results |
T315 |
319-325 |
VBD |
denotes |
showed |
T316 |
302-306 |
IN |
denotes |
from |
T317 |
307-313 |
NN |
denotes |
mutant |
T318 |
314-318 |
NNS |
denotes |
mice |
T319 |
326-333 |
JJ |
denotes |
altered |
T320 |
344-355 |
NN |
denotes |
propagation |
T321 |
334-338 |
NN |
denotes |
Ca2+ |
T322 |
339-343 |
NN |
denotes |
wave |
T323 |
338-339 |
HYPH |
denotes |
- |
T324 |
356-358 |
IN |
denotes |
in |
T325 |
359-369 |
NNS |
denotes |
astrocytes |
T326 |
369-370 |
. |
denotes |
. |
T327 |
370-629 |
sentence |
denotes |
As the appearance and distribution of Annexin A7 during brain development has not been investigated so far, we focused on the distribution of Annexin A7 protein during mouse embryogenesis in the developing central nervous system and in the adult mouse brain. |
T328 |
371-373 |
IN |
denotes |
As |
T329 |
458-470 |
VBN |
denotes |
investigated |
T330 |
374-377 |
DT |
denotes |
the |
T331 |
378-388 |
NN |
denotes |
appearance |
T332 |
389-392 |
CC |
denotes |
and |
T333 |
393-405 |
NN |
denotes |
distribution |
T334 |
406-408 |
IN |
denotes |
of |
T335 |
409-416 |
NN |
denotes |
Annexin |
T336 |
417-419 |
NN |
denotes |
A7 |
T337 |
420-426 |
IN |
denotes |
during |
T338 |
427-432 |
NN |
denotes |
brain |
T339 |
433-444 |
NN |
denotes |
development |
T340 |
445-448 |
VBZ |
denotes |
has |
T341 |
449-452 |
RB |
denotes |
not |
T342 |
453-457 |
VBN |
denotes |
been |
T343 |
482-489 |
VBD |
denotes |
focused |
T344 |
471-473 |
RB |
denotes |
so |
T345 |
474-477 |
RB |
denotes |
far |
T346 |
477-479 |
, |
denotes |
, |
T347 |
479-481 |
PRP |
denotes |
we |
T348 |
490-492 |
IN |
denotes |
on |
T349 |
493-496 |
DT |
denotes |
the |
T350 |
497-509 |
NN |
denotes |
distribution |
T351 |
510-512 |
IN |
denotes |
of |
T352 |
513-520 |
NN |
denotes |
Annexin |
T353 |
521-523 |
NN |
denotes |
A7 |
T354 |
524-531 |
NN |
denotes |
protein |
T355 |
532-538 |
IN |
denotes |
during |
T356 |
539-544 |
NN |
denotes |
mouse |
T357 |
545-558 |
NN |
denotes |
embryogenesis |
T358 |
559-561 |
IN |
denotes |
in |
T359 |
562-565 |
DT |
denotes |
the |
T360 |
593-599 |
NN |
denotes |
system |
T361 |
566-576 |
VBG |
denotes |
developing |
T362 |
577-584 |
JJ |
denotes |
central |
T363 |
585-592 |
JJ |
denotes |
nervous |
T364 |
600-603 |
CC |
denotes |
and |
T365 |
604-606 |
IN |
denotes |
in |
T366 |
607-610 |
DT |
denotes |
the |
T367 |
623-628 |
NN |
denotes |
brain |
T368 |
611-616 |
JJ |
denotes |
adult |
T369 |
617-622 |
NN |
denotes |
mouse |
T370 |
628-629 |
. |
denotes |
. |
T371 |
629-638 |
sentence |
denotes |
Results |
T372 |
631-638 |
NNS |
denotes |
Results |
T373 |
638-782 |
sentence |
denotes |
Annexin A7 is expressed in cells of the developing brain where a change in its subcellular localization from cytoplasm to nucleus was observed. |
T374 |
639-646 |
NN |
denotes |
Annexin |
T375 |
647-649 |
NN |
denotes |
A7 |
T376 |
653-662 |
VBN |
denotes |
expressed |
T377 |
650-652 |
VBZ |
denotes |
is |
T378 |
663-665 |
IN |
denotes |
in |
T379 |
666-671 |
NNS |
denotes |
cells |
T380 |
672-674 |
IN |
denotes |
of |
T381 |
675-678 |
DT |
denotes |
the |
T382 |
690-695 |
NN |
denotes |
brain |
T383 |
679-689 |
VBG |
denotes |
developing |
T384 |
696-701 |
WRB |
denotes |
where |
T385 |
773-781 |
VBN |
denotes |
observed |
T386 |
702-703 |
DT |
denotes |
a |
T387 |
704-710 |
NN |
denotes |
change |
T388 |
711-713 |
IN |
denotes |
in |
T389 |
714-717 |
PRP$ |
denotes |
its |
T390 |
730-742 |
NN |
denotes |
localization |
T391 |
718-729 |
JJ |
denotes |
subcellular |
T392 |
743-747 |
IN |
denotes |
from |
T393 |
748-757 |
NN |
denotes |
cytoplasm |
T394 |
758-760 |
IN |
denotes |
to |
T395 |
761-768 |
NN |
denotes |
nucleus |
T396 |
769-772 |
VBD |
denotes |
was |
T397 |
781-782 |
. |
denotes |
. |
T398 |
782-869 |
sentence |
denotes |
In the adult CNS, the subcellular distribution of Annexin A7 depends on the cell type. |
T399 |
783-785 |
IN |
denotes |
In |
T400 |
844-851 |
VBZ |
denotes |
depends |
T401 |
786-789 |
DT |
denotes |
the |
T402 |
796-799 |
NN |
denotes |
CNS |
T403 |
790-795 |
JJ |
denotes |
adult |
T404 |
799-801 |
, |
denotes |
, |
T405 |
801-804 |
DT |
denotes |
the |
T406 |
817-829 |
NN |
denotes |
distribution |
T407 |
805-816 |
JJ |
denotes |
subcellular |
T408 |
830-832 |
IN |
denotes |
of |
T409 |
833-840 |
NN |
denotes |
Annexin |
T410 |
841-843 |
NN |
denotes |
A7 |
T411 |
852-854 |
IN |
denotes |
on |
T412 |
855-858 |
DT |
denotes |
the |
T413 |
864-868 |
NN |
denotes |
type |
T414 |
859-863 |
NN |
denotes |
cell |
T415 |
868-869 |
. |
denotes |
. |
T416 |
869-992 |
sentence |
denotes |
By immunohistochemistry analysis Annexin A7 was detected in the cytosol of undifferentiated cells at embryonic days E5–E8. |
T417 |
870-872 |
IN |
denotes |
By |
T418 |
918-926 |
VBN |
denotes |
detected |
T419 |
873-893 |
NN |
denotes |
immunohistochemistry |
T420 |
894-902 |
NN |
denotes |
analysis |
T421 |
903-910 |
NN |
denotes |
Annexin |
T422 |
911-913 |
NN |
denotes |
A7 |
T423 |
914-917 |
VBD |
denotes |
was |
T424 |
927-929 |
IN |
denotes |
in |
T425 |
930-933 |
DT |
denotes |
the |
T426 |
934-941 |
NN |
denotes |
cytosol |
T427 |
942-944 |
IN |
denotes |
of |
T428 |
945-961 |
JJ |
denotes |
undifferentiated |
T429 |
962-967 |
NNS |
denotes |
cells |
T430 |
968-970 |
IN |
denotes |
at |
T431 |
971-980 |
JJ |
denotes |
embryonic |
T432 |
986-988 |
NN |
denotes |
E5 |
T433 |
981-985 |
NNS |
denotes |
days |
T434 |
988-989 |
SYM |
denotes |
– |
T435 |
989-991 |
NN |
denotes |
E8 |
T436 |
991-992 |
. |
denotes |
. |
T437 |
992-1149 |
sentence |
denotes |
At E11–E15 the protein is still present in the cytosol of cells predominantly located in the ventricular germinative zone surrounding the lateral ventricle. |
T438 |
993-995 |
IN |
denotes |
At |
T439 |
1016-1018 |
VBZ |
denotes |
is |
T440 |
996-999 |
NN |
denotes |
E11 |
T441 |
999-1000 |
SYM |
denotes |
– |
T442 |
1000-1003 |
NN |
denotes |
E15 |
T443 |
1004-1007 |
DT |
denotes |
the |
T444 |
1008-1015 |
NN |
denotes |
protein |
T445 |
1019-1024 |
RB |
denotes |
still |
T446 |
1025-1032 |
JJ |
denotes |
present |
T447 |
1033-1035 |
IN |
denotes |
in |
T448 |
1036-1039 |
DT |
denotes |
the |
T449 |
1040-1047 |
NN |
denotes |
cytosol |
T450 |
1048-1050 |
IN |
denotes |
of |
T451 |
1051-1056 |
NNS |
denotes |
cells |
T452 |
1057-1070 |
RB |
denotes |
predominantly |
T453 |
1071-1078 |
VBN |
denotes |
located |
T454 |
1079-1081 |
IN |
denotes |
in |
T455 |
1082-1085 |
DT |
denotes |
the |
T456 |
1110-1114 |
NN |
denotes |
zone |
T457 |
1086-1097 |
JJ |
denotes |
ventricular |
T458 |
1098-1109 |
JJ |
denotes |
germinative |
T459 |
1115-1126 |
VBG |
denotes |
surrounding |
T460 |
1127-1130 |
DT |
denotes |
the |
T461 |
1139-1148 |
NN |
denotes |
ventricle |
T462 |
1131-1138 |
JJ |
denotes |
lateral |
T463 |
1148-1149 |
. |
denotes |
. |
T464 |
1149-1286 |
sentence |
denotes |
Later on, at embryonic day E16, Annexin A7 in cells of the intermediate and marginal zone of the neopallium translocates to the nucleus. |
T465 |
1150-1155 |
RB |
denotes |
Later |
T466 |
1156-1158 |
RB |
denotes |
on |
T467 |
1258-1270 |
VBZ |
denotes |
translocates |
T468 |
1158-1160 |
, |
denotes |
, |
T469 |
1160-1162 |
IN |
denotes |
at |
T470 |
1163-1172 |
JJ |
denotes |
embryonic |
T471 |
1177-1180 |
NN |
denotes |
E16 |
T472 |
1173-1176 |
NN |
denotes |
day |
T473 |
1180-1182 |
, |
denotes |
, |
T474 |
1182-1189 |
NN |
denotes |
Annexin |
T475 |
1190-1192 |
NN |
denotes |
A7 |
T476 |
1193-1195 |
IN |
denotes |
in |
T477 |
1196-1201 |
NNS |
denotes |
cells |
T478 |
1202-1204 |
IN |
denotes |
of |
T479 |
1205-1208 |
DT |
denotes |
the |
T480 |
1235-1239 |
NN |
denotes |
zone |
T481 |
1209-1221 |
JJ |
denotes |
intermediate |
T482 |
1222-1225 |
CC |
denotes |
and |
T483 |
1226-1234 |
JJ |
denotes |
marginal |
T484 |
1240-1242 |
IN |
denotes |
of |
T485 |
1243-1246 |
DT |
denotes |
the |
T486 |
1247-1257 |
NN |
denotes |
neopallium |
T487 |
1271-1273 |
IN |
denotes |
to |
T488 |
1274-1277 |
DT |
denotes |
the |
T489 |
1278-1285 |
NN |
denotes |
nucleus |
T490 |
1285-1286 |
. |
denotes |
. |
T491 |
1286-1485 |
sentence |
denotes |
Neuronal cells of all areas in the adult brain present Annexin A7 in the nucleus, whereas glial fibrillary acidic protein (GFAP)-positive astrocytes exhibit both, a cytoplasmic and nuclear staining. |
T492 |
1287-1295 |
JJ |
denotes |
Neuronal |
T493 |
1296-1301 |
NNS |
denotes |
cells |
T494 |
1334-1341 |
VBP |
denotes |
present |
T495 |
1302-1304 |
IN |
denotes |
of |
T496 |
1305-1308 |
DT |
denotes |
all |
T497 |
1309-1314 |
NNS |
denotes |
areas |
T498 |
1315-1317 |
IN |
denotes |
in |
T499 |
1318-1321 |
DT |
denotes |
the |
T500 |
1328-1333 |
NN |
denotes |
brain |
T501 |
1322-1327 |
JJ |
denotes |
adult |
T502 |
1342-1349 |
NN |
denotes |
Annexin |
T503 |
1350-1352 |
NN |
denotes |
A7 |
T504 |
1353-1355 |
IN |
denotes |
in |
T505 |
1356-1359 |
DT |
denotes |
the |
T506 |
1360-1367 |
NN |
denotes |
nucleus |
T507 |
1367-1369 |
, |
denotes |
, |
T508 |
1369-1376 |
IN |
denotes |
whereas |
T509 |
1436-1443 |
VBP |
denotes |
exhibit |
T510 |
1377-1382 |
JJ |
denotes |
glial |
T511 |
1401-1408 |
NN |
denotes |
protein |
T512 |
1383-1393 |
JJ |
denotes |
fibrillary |
T513 |
1394-1400 |
JJ |
denotes |
acidic |
T514 |
1416-1424 |
JJ |
denotes |
positive |
T515 |
1409-1410 |
-LRB- |
denotes |
( |
T516 |
1410-1414 |
NN |
denotes |
GFAP |
T517 |
1414-1415 |
-RRB- |
denotes |
) |
T518 |
1415-1416 |
HYPH |
denotes |
- |
T519 |
1425-1435 |
NNS |
denotes |
astrocytes |
T520 |
1444-1448 |
CC |
denotes |
both |
T521 |
1452-1463 |
JJ |
denotes |
cytoplasmic |
T522 |
1448-1450 |
, |
denotes |
, |
T523 |
1450-1451 |
DT |
denotes |
a |
T524 |
1464-1467 |
CC |
denotes |
and |
T525 |
1468-1475 |
JJ |
denotes |
nuclear |
T526 |
1476-1484 |
NN |
denotes |
staining |
T527 |
1484-1485 |
. |
denotes |
. |
T528 |
1485-1638 |
sentence |
denotes |
The presence of nuclear Annexin A7 was confirmed by extraction of the nucleoplasm from isolated nuclei obtained from neuronal and astroglial cell lines. |
T529 |
1486-1489 |
DT |
denotes |
The |
T530 |
1490-1498 |
NN |
denotes |
presence |
T531 |
1525-1534 |
VBN |
denotes |
confirmed |
T532 |
1499-1501 |
IN |
denotes |
of |
T533 |
1502-1509 |
JJ |
denotes |
nuclear |
T534 |
1518-1520 |
NN |
denotes |
A7 |
T535 |
1510-1517 |
NN |
denotes |
Annexin |
T536 |
1521-1524 |
VBD |
denotes |
was |
T537 |
1535-1537 |
IN |
denotes |
by |
T538 |
1538-1548 |
NN |
denotes |
extraction |
T539 |
1549-1551 |
IN |
denotes |
of |
T540 |
1552-1555 |
DT |
denotes |
the |
T541 |
1556-1567 |
NN |
denotes |
nucleoplasm |
T542 |
1568-1572 |
IN |
denotes |
from |
T543 |
1573-1581 |
VBN |
denotes |
isolated |
T544 |
1582-1588 |
NNS |
denotes |
nuclei |
T545 |
1589-1597 |
VBN |
denotes |
obtained |
T546 |
1598-1602 |
IN |
denotes |
from |
T547 |
1603-1611 |
JJ |
denotes |
neuronal |
T548 |
1632-1637 |
NNS |
denotes |
lines |
T549 |
1612-1615 |
CC |
denotes |
and |
T550 |
1616-1626 |
JJ |
denotes |
astroglial |
T551 |
1627-1631 |
NN |
denotes |
cell |
T552 |
1637-1638 |
. |
denotes |
. |
T553 |
1638-1650 |
sentence |
denotes |
Conclusion |
T554 |
1640-1650 |
NN |
denotes |
Conclusion |
T555 |
1650-1834 |
sentence |
denotes |
We have demonstrated a translocation of Annexin A7 to nuclei of cells in early murine brain development and the presence of Annexin A7 in nuclei of neuronal cells in the adult animal. |
T556 |
1651-1653 |
PRP |
denotes |
We |
T557 |
1659-1671 |
VBN |
denotes |
demonstrated |
T558 |
1654-1658 |
VBP |
denotes |
have |
T559 |
1672-1673 |
DT |
denotes |
a |
T560 |
1674-1687 |
NN |
denotes |
translocation |
T561 |
1688-1690 |
IN |
denotes |
of |
T562 |
1691-1698 |
NN |
denotes |
Annexin |
T563 |
1699-1701 |
NN |
denotes |
A7 |
T564 |
1702-1704 |
IN |
denotes |
to |
T565 |
1705-1711 |
NNS |
denotes |
nuclei |
T566 |
1712-1714 |
IN |
denotes |
of |
T567 |
1715-1720 |
NNS |
denotes |
cells |
T568 |
1721-1723 |
IN |
denotes |
in |
T569 |
1724-1729 |
JJ |
denotes |
early |
T570 |
1743-1754 |
NN |
denotes |
development |
T571 |
1730-1736 |
JJ |
denotes |
murine |
T572 |
1737-1742 |
NN |
denotes |
brain |
T573 |
1755-1758 |
CC |
denotes |
and |
T574 |
1759-1762 |
DT |
denotes |
the |
T575 |
1763-1771 |
NN |
denotes |
presence |
T576 |
1772-1774 |
IN |
denotes |
of |
T577 |
1775-1782 |
NN |
denotes |
Annexin |
T578 |
1783-1785 |
NN |
denotes |
A7 |
T579 |
1786-1788 |
IN |
denotes |
in |
T580 |
1789-1795 |
NNS |
denotes |
nuclei |
T581 |
1796-1798 |
IN |
denotes |
of |
T582 |
1799-1807 |
JJ |
denotes |
neuronal |
T583 |
1808-1813 |
NNS |
denotes |
cells |
T584 |
1814-1816 |
IN |
denotes |
in |
T585 |
1817-1820 |
DT |
denotes |
the |
T586 |
1827-1833 |
NN |
denotes |
animal |
T587 |
1821-1826 |
JJ |
denotes |
adult |
T588 |
1833-1834 |
. |
denotes |
. |
T589 |
1834-1929 |
sentence |
denotes |
The role of Annexin A7 in nuclei of differentiating and mature neuronal cells remains elusive. |
T590 |
1835-1838 |
DT |
denotes |
The |
T591 |
1839-1843 |
NN |
denotes |
role |
T592 |
1913-1920 |
VBZ |
denotes |
remains |
T593 |
1844-1846 |
IN |
denotes |
of |
T594 |
1847-1854 |
NN |
denotes |
Annexin |
T595 |
1855-1857 |
NN |
denotes |
A7 |
T596 |
1858-1860 |
IN |
denotes |
in |
T597 |
1861-1867 |
NNS |
denotes |
nuclei |
T598 |
1868-1870 |
IN |
denotes |
of |
T599 |
1871-1886 |
VBG |
denotes |
differentiating |
T600 |
1907-1912 |
NNS |
denotes |
cells |
T601 |
1887-1890 |
CC |
denotes |
and |
T602 |
1891-1897 |
JJ |
denotes |
mature |
T603 |
1898-1906 |
JJ |
denotes |
neuronal |
T604 |
1921-1928 |
JJ |
denotes |
elusive |
T605 |
1928-1929 |
. |
denotes |
. |
T1249 |
1942-1950 |
NNS |
denotes |
Annexins |
T1250 |
1951-1955 |
VBP |
denotes |
form |
T1251 |
1956-1957 |
DT |
denotes |
a |
T1252 |
1958-1964 |
NN |
denotes |
family |
T1253 |
1965-1967 |
IN |
denotes |
of |
T1254 |
1968-1980 |
RB |
denotes |
structurally |
T1255 |
1981-1988 |
JJ |
denotes |
related |
T1256 |
1989-1997 |
NN |
denotes |
proteins |
T1257 |
1997-1999 |
, |
denotes |
, |
T1258 |
1999-2004 |
WDT |
denotes |
which |
T1259 |
2005-2009 |
VBP |
denotes |
bind |
T1260 |
2010-2012 |
IN |
denotes |
to |
T1261 |
2013-2023 |
RB |
denotes |
negatively |
T1262 |
2024-2031 |
VBN |
denotes |
charged |
T1263 |
2032-2045 |
NNS |
denotes |
phospholipids |
T1264 |
2046-2048 |
IN |
denotes |
in |
T1265 |
2049-2050 |
DT |
denotes |
a |
T1266 |
2066-2072 |
NN |
denotes |
manner |
T1267 |
2051-2055 |
NN |
denotes |
Ca2+ |
T1268 |
2056-2065 |
JJ |
denotes |
dependent |
T1269 |
2055-2056 |
HYPH |
denotes |
- |
T1270 |
2073-2074 |
-LRB- |
denotes |
[ |
T1271 |
2074-2075 |
CD |
denotes |
1 |
T1272 |
2075-2076 |
-RRB- |
denotes |
] |
T1273 |
2076-2077 |
. |
denotes |
. |
T1274 |
2077-2246 |
sentence |
denotes |
They are characterized by a bipartite structure with a conserved C-terminal core domain and a unique N-terminal domain that varies in length and amino acid composition. |
T1275 |
2078-2082 |
PRP |
denotes |
They |
T1276 |
2087-2100 |
VBN |
denotes |
characterized |
T1277 |
2083-2086 |
VBP |
denotes |
are |
T1278 |
2101-2103 |
IN |
denotes |
by |
T1279 |
2104-2105 |
DT |
denotes |
a |
T1280 |
2116-2125 |
NN |
denotes |
structure |
T1281 |
2106-2115 |
JJ |
denotes |
bipartite |
T1282 |
2126-2130 |
IN |
denotes |
with |
T1283 |
2131-2132 |
DT |
denotes |
a |
T1284 |
2159-2165 |
NN |
denotes |
domain |
T1285 |
2133-2142 |
VBN |
denotes |
conserved |
T1286 |
2143-2144 |
NN |
denotes |
C |
T1287 |
2145-2153 |
JJ |
denotes |
terminal |
T1288 |
2144-2145 |
HYPH |
denotes |
- |
T1289 |
2154-2158 |
NN |
denotes |
core |
T1290 |
2166-2169 |
CC |
denotes |
and |
T1291 |
2170-2171 |
DT |
denotes |
a |
T1292 |
2190-2196 |
NN |
denotes |
domain |
T1293 |
2172-2178 |
JJ |
denotes |
unique |
T1294 |
2179-2180 |
NN |
denotes |
N |
T1295 |
2181-2189 |
JJ |
denotes |
terminal |
T1296 |
2180-2181 |
HYPH |
denotes |
- |
T1297 |
2197-2201 |
WDT |
denotes |
that |
T1298 |
2202-2208 |
VBZ |
denotes |
varies |
T1299 |
2209-2211 |
IN |
denotes |
in |
T1300 |
2212-2218 |
NN |
denotes |
length |
T1301 |
2219-2222 |
CC |
denotes |
and |
T1302 |
2223-2228 |
NN |
denotes |
amino |
T1303 |
2229-2233 |
NN |
denotes |
acid |
T1304 |
2234-2245 |
NN |
denotes |
composition |
T1305 |
2245-2246 |
. |
denotes |
. |
T1306 |
2246-2436 |
sentence |
denotes |
The C-terminal domain is formed by either a four- or eightfold repeat of approximately 70 amino acids, each repeat carrying a Ca2+-binding site, and is responsible for phospholipid binding. |
T1307 |
2247-2250 |
DT |
denotes |
The |
T1308 |
2262-2268 |
NN |
denotes |
domain |
T1309 |
2251-2252 |
NN |
denotes |
C |
T1310 |
2253-2261 |
JJ |
denotes |
terminal |
T1311 |
2252-2253 |
HYPH |
denotes |
- |
T1312 |
2272-2278 |
VBN |
denotes |
formed |
T1313 |
2269-2271 |
VBZ |
denotes |
is |
T1314 |
2279-2281 |
IN |
denotes |
by |
T1315 |
2282-2288 |
CC |
denotes |
either |
T1316 |
2310-2316 |
NN |
denotes |
repeat |
T1317 |
2289-2290 |
DT |
denotes |
a |
T1318 |
2291-2295 |
CD |
denotes |
four |
T1319 |
2295-2296 |
HYPH |
denotes |
- |
T1320 |
2297-2299 |
CC |
denotes |
or |
T1321 |
2300-2309 |
JJ |
denotes |
eightfold |
T1322 |
2317-2319 |
IN |
denotes |
of |
T1323 |
2320-2333 |
RB |
denotes |
approximately |
T1324 |
2334-2336 |
CD |
denotes |
70 |
T1325 |
2343-2348 |
NNS |
denotes |
acids |
T1326 |
2337-2342 |
NN |
denotes |
amino |
T1327 |
2348-2350 |
, |
denotes |
, |
T1328 |
2350-2354 |
DT |
denotes |
each |
T1329 |
2355-2361 |
NN |
denotes |
repeat |
T1330 |
2362-2370 |
VBG |
denotes |
carrying |
T1331 |
2371-2372 |
DT |
denotes |
a |
T1332 |
2386-2390 |
NN |
denotes |
site |
T1333 |
2373-2377 |
NN |
denotes |
Ca2+ |
T1334 |
2377-2378 |
HYPH |
denotes |
- |
T1335 |
2378-2385 |
VBG |
denotes |
binding |
T1336 |
2390-2392 |
, |
denotes |
, |
T1337 |
2392-2395 |
CC |
denotes |
and |
T1338 |
2396-2398 |
VBZ |
denotes |
is |
T1339 |
2399-2410 |
JJ |
denotes |
responsible |
T1340 |
2411-2414 |
IN |
denotes |
for |
T1341 |
2415-2427 |
NN |
denotes |
phospholipid |
T1342 |
2428-2435 |
NN |
denotes |
binding |
T1343 |
2435-2436 |
. |
denotes |
. |
T1344 |
2436-2531 |
sentence |
denotes |
The N-terminal regions are thought to confer functional diversity to the annexin proteins [2]. |
T1345 |
2437-2440 |
DT |
denotes |
The |
T1346 |
2452-2459 |
NNS |
denotes |
regions |
T1347 |
2441-2442 |
NN |
denotes |
N |
T1348 |
2443-2451 |
JJ |
denotes |
terminal |
T1349 |
2442-2443 |
HYPH |
denotes |
- |
T1350 |
2464-2471 |
VBN |
denotes |
thought |
T1351 |
2460-2463 |
VBP |
denotes |
are |
T1352 |
2472-2474 |
TO |
denotes |
to |
T1353 |
2475-2481 |
VB |
denotes |
confer |
T1354 |
2482-2492 |
JJ |
denotes |
functional |
T1355 |
2493-2502 |
NN |
denotes |
diversity |
T1356 |
2503-2505 |
IN |
denotes |
to |
T1357 |
2506-2509 |
DT |
denotes |
the |
T1358 |
2518-2526 |
NN |
denotes |
proteins |
T1359 |
2510-2517 |
NN |
denotes |
annexin |
T1360 |
2527-2528 |
-LRB- |
denotes |
[ |
T1361 |
2528-2529 |
CD |
denotes |
2 |
T1362 |
2529-2530 |
-RRB- |
denotes |
] |
T1363 |
2530-2531 |
. |
denotes |
. |
T1364 |
2531-2646 |
sentence |
denotes |
The biochemical features in vitro were analyzed extensively, but the in vivo functions of annexins remain unclear. |
T1365 |
2532-2535 |
DT |
denotes |
The |
T1366 |
2548-2556 |
NNS |
denotes |
features |
T1367 |
2536-2547 |
JJ |
denotes |
biochemical |
T1368 |
2571-2579 |
VBN |
denotes |
analyzed |
T1369 |
2557-2559 |
FW |
denotes |
in |
T1370 |
2560-2565 |
FW |
denotes |
vitro |
T1371 |
2566-2570 |
VBD |
denotes |
were |
T1372 |
2580-2591 |
RB |
denotes |
extensively |
T1373 |
2591-2593 |
, |
denotes |
, |
T1374 |
2593-2596 |
CC |
denotes |
but |
T1375 |
2597-2600 |
DT |
denotes |
the |
T1376 |
2609-2618 |
NNS |
denotes |
functions |
T1377 |
2601-2603 |
FW |
denotes |
in |
T1378 |
2604-2608 |
FW |
denotes |
vivo |
T1379 |
2631-2637 |
VBP |
denotes |
remain |
T1380 |
2619-2621 |
IN |
denotes |
of |
T1381 |
2622-2630 |
NNS |
denotes |
annexins |
T1382 |
2638-2645 |
JJ |
denotes |
unclear |
T1383 |
2645-2646 |
. |
denotes |
. |
T1384 |
2646-2844 |
sentence |
denotes |
Annexin A7, the first annexin to be described, was isolated as the agent that mediated aggregation of chromaffin granules and fusion of membranes and phospholipids in the presence of Ca2+-ions [3]. |
T1385 |
2647-2654 |
NN |
denotes |
Annexin |
T1386 |
2655-2657 |
NN |
denotes |
A7 |
T1387 |
2698-2706 |
VBN |
denotes |
isolated |
T1388 |
2657-2659 |
, |
denotes |
, |
T1389 |
2659-2662 |
DT |
denotes |
the |
T1390 |
2669-2676 |
NN |
denotes |
annexin |
T1391 |
2663-2668 |
JJ |
denotes |
first |
T1392 |
2677-2679 |
TO |
denotes |
to |
T1393 |
2683-2692 |
VBN |
denotes |
described |
T1394 |
2680-2682 |
VB |
denotes |
be |
T1395 |
2692-2694 |
, |
denotes |
, |
T1396 |
2694-2697 |
VBD |
denotes |
was |
T1397 |
2707-2709 |
IN |
denotes |
as |
T1398 |
2710-2713 |
DT |
denotes |
the |
T1399 |
2714-2719 |
NN |
denotes |
agent |
T1400 |
2720-2724 |
WDT |
denotes |
that |
T1401 |
2725-2733 |
VBD |
denotes |
mediated |
T1402 |
2734-2745 |
NN |
denotes |
aggregation |
T1403 |
2746-2748 |
IN |
denotes |
of |
T1404 |
2749-2759 |
NN |
denotes |
chromaffin |
T1405 |
2760-2768 |
NNS |
denotes |
granules |
T1406 |
2769-2772 |
CC |
denotes |
and |
T1407 |
2773-2779 |
NN |
denotes |
fusion |
T1408 |
2780-2782 |
IN |
denotes |
of |
T1409 |
2783-2792 |
NNS |
denotes |
membranes |
T1410 |
2793-2796 |
CC |
denotes |
and |
T1411 |
2797-2810 |
NNS |
denotes |
phospholipids |
T1412 |
2811-2813 |
IN |
denotes |
in |
T1413 |
2814-2817 |
DT |
denotes |
the |
T1414 |
2818-2826 |
NN |
denotes |
presence |
T1415 |
2827-2829 |
IN |
denotes |
of |
T1416 |
2830-2834 |
NN |
denotes |
Ca2+ |
T1417 |
2835-2839 |
NNS |
denotes |
ions |
T1418 |
2834-2835 |
HYPH |
denotes |
- |
T1419 |
2840-2841 |
-LRB- |
denotes |
[ |
T1420 |
2841-2842 |
CD |
denotes |
3 |
T1421 |
2842-2843 |
-RRB- |
denotes |
] |
T1422 |
2843-2844 |
. |
denotes |
. |
T1423 |
2844-3100 |
sentence |
denotes |
Expression studies demonstrated the distribution of Annexin A7 in a wide variety of tissues and cells mainly enriched in the cytosol in close association with membranous structures, but it was also described in the nucleus of adrenal chromaffin cells [4]. |
T1424 |
2845-2855 |
NN |
denotes |
Expression |
T1425 |
2856-2863 |
NNS |
denotes |
studies |
T1426 |
2864-2876 |
VBD |
denotes |
demonstrated |
T1427 |
2877-2880 |
DT |
denotes |
the |
T1428 |
2881-2893 |
NN |
denotes |
distribution |
T1429 |
2894-2896 |
IN |
denotes |
of |
T1430 |
2897-2904 |
NN |
denotes |
Annexin |
T1431 |
2905-2907 |
NN |
denotes |
A7 |
T1432 |
2908-2910 |
IN |
denotes |
in |
T1433 |
2911-2912 |
DT |
denotes |
a |
T1434 |
2918-2925 |
NN |
denotes |
variety |
T1435 |
2913-2917 |
JJ |
denotes |
wide |
T1436 |
2926-2928 |
IN |
denotes |
of |
T1437 |
2929-2936 |
NNS |
denotes |
tissues |
T1438 |
2937-2940 |
CC |
denotes |
and |
T1439 |
2941-2946 |
NNS |
denotes |
cells |
T1440 |
2947-2953 |
RB |
denotes |
mainly |
T1441 |
2954-2962 |
VBN |
denotes |
enriched |
T1442 |
2963-2965 |
IN |
denotes |
in |
T1443 |
2966-2969 |
DT |
denotes |
the |
T1444 |
2970-2977 |
NN |
denotes |
cytosol |
T1445 |
2978-2980 |
IN |
denotes |
in |
T1446 |
2981-2986 |
JJ |
denotes |
close |
T1447 |
2987-2998 |
NN |
denotes |
association |
T1448 |
2999-3003 |
IN |
denotes |
with |
T1449 |
3004-3014 |
JJ |
denotes |
membranous |
T1450 |
3015-3025 |
NNS |
denotes |
structures |
T1451 |
3025-3027 |
, |
denotes |
, |
T1452 |
3027-3030 |
CC |
denotes |
but |
T1453 |
3031-3033 |
PRP |
denotes |
it |
T1454 |
3043-3052 |
VBN |
denotes |
described |
T1455 |
3034-3037 |
VBD |
denotes |
was |
T1456 |
3038-3042 |
RB |
denotes |
also |
T1457 |
3053-3055 |
IN |
denotes |
in |
T1458 |
3056-3059 |
DT |
denotes |
the |
T1459 |
3060-3067 |
NN |
denotes |
nucleus |
T1460 |
3068-3070 |
IN |
denotes |
of |
T1461 |
3071-3078 |
JJ |
denotes |
adrenal |
T1462 |
3090-3095 |
NNS |
denotes |
cells |
T1463 |
3079-3089 |
NN |
denotes |
chromaffin |
T1464 |
3096-3097 |
-LRB- |
denotes |
[ |
T1465 |
3097-3098 |
CD |
denotes |
4 |
T1466 |
3098-3099 |
-RRB- |
denotes |
] |
T1467 |
3099-3100 |
. |
denotes |
. |
T1468 |
3100-3247 |
sentence |
denotes |
The presence of an alternatively spliced cassette exon gives rise to two Annexin A7 isoforms corresponding in molecular mass to 47 kDa and 51 kDa. |
T1469 |
3101-3104 |
DT |
denotes |
The |
T1470 |
3105-3113 |
NN |
denotes |
presence |
T1471 |
3156-3161 |
VBZ |
denotes |
gives |
T1472 |
3114-3116 |
IN |
denotes |
of |
T1473 |
3117-3119 |
DT |
denotes |
an |
T1474 |
3151-3155 |
NN |
denotes |
exon |
T1475 |
3120-3133 |
RB |
denotes |
alternatively |
T1476 |
3134-3141 |
VBN |
denotes |
spliced |
T1477 |
3142-3150 |
NN |
denotes |
cassette |
T1478 |
3162-3166 |
NN |
denotes |
rise |
T1479 |
3167-3169 |
IN |
denotes |
to |
T1480 |
3170-3173 |
CD |
denotes |
two |
T1481 |
3185-3193 |
NNS |
denotes |
isoforms |
T1482 |
3174-3181 |
NN |
denotes |
Annexin |
T1483 |
3182-3184 |
NN |
denotes |
A7 |
T1484 |
3194-3207 |
VBG |
denotes |
corresponding |
T1485 |
3208-3210 |
IN |
denotes |
in |
T1486 |
3211-3220 |
JJ |
denotes |
molecular |
T1487 |
3221-3225 |
NN |
denotes |
mass |
T1488 |
3226-3228 |
IN |
denotes |
to |
T1489 |
3229-3231 |
CD |
denotes |
47 |
T1490 |
3232-3235 |
NN |
denotes |
kDa |
T1491 |
3236-3239 |
CC |
denotes |
and |
T1492 |
3240-3242 |
CD |
denotes |
51 |
T1493 |
3243-3246 |
NN |
denotes |
kDa |
T1494 |
3246-3247 |
. |
denotes |
. |
T1495 |
3247-3344 |
sentence |
denotes |
The isoforms differ in their N-terminal domain and exhibit a tissue-specific expression pattern. |
T1496 |
3248-3251 |
DT |
denotes |
The |
T1497 |
3252-3260 |
NNS |
denotes |
isoforms |
T1498 |
3261-3267 |
VBP |
denotes |
differ |
T1499 |
3268-3270 |
IN |
denotes |
in |
T1500 |
3271-3276 |
PRP$ |
denotes |
their |
T1501 |
3288-3294 |
NN |
denotes |
domain |
T1502 |
3277-3278 |
NN |
denotes |
N |
T1503 |
3279-3287 |
JJ |
denotes |
terminal |
T1504 |
3278-3279 |
HYPH |
denotes |
- |
T1505 |
3295-3298 |
CC |
denotes |
and |
T1506 |
3299-3306 |
VBP |
denotes |
exhibit |
T1507 |
3307-3308 |
DT |
denotes |
a |
T1508 |
3336-3343 |
NN |
denotes |
pattern |
T1509 |
3309-3315 |
NN |
denotes |
tissue |
T1510 |
3316-3324 |
JJ |
denotes |
specific |
T1511 |
3315-3316 |
HYPH |
denotes |
- |
T1512 |
3325-3335 |
NN |
denotes |
expression |
T1513 |
3343-3344 |
. |
denotes |
. |
T1514 |
3344-3466 |
sentence |
denotes |
The 47 kDa isoform is present in all tissues except for skeletal muscle, where the 51 kDa isoform is exclusively present. |
T1515 |
3345-3348 |
DT |
denotes |
The |
T1516 |
3356-3363 |
NN |
denotes |
isoform |
T1517 |
3349-3351 |
CD |
denotes |
47 |
T1518 |
3352-3355 |
NN |
denotes |
kDa |
T1519 |
3364-3366 |
VBZ |
denotes |
is |
T1520 |
3367-3374 |
JJ |
denotes |
present |
T1521 |
3375-3377 |
IN |
denotes |
in |
T1522 |
3378-3381 |
DT |
denotes |
all |
T1523 |
3382-3389 |
NNS |
denotes |
tissues |
T1524 |
3390-3396 |
IN |
denotes |
except |
T1525 |
3397-3400 |
IN |
denotes |
for |
T1526 |
3401-3409 |
JJ |
denotes |
skeletal |
T1527 |
3410-3416 |
NN |
denotes |
muscle |
T1528 |
3416-3418 |
, |
denotes |
, |
T1529 |
3418-3423 |
WRB |
denotes |
where |
T1530 |
3443-3445 |
VBZ |
denotes |
is |
T1531 |
3424-3427 |
DT |
denotes |
the |
T1532 |
3435-3442 |
NN |
denotes |
isoform |
T1533 |
3428-3430 |
CD |
denotes |
51 |
T1534 |
3431-3434 |
NN |
denotes |
kDa |
T1535 |
3446-3457 |
RB |
denotes |
exclusively |
T1536 |
3458-3465 |
JJ |
denotes |
present |
T1537 |
3465-3466 |
. |
denotes |
. |
T1538 |
3466-3542 |
sentence |
denotes |
Heart muscle, brain tissue and red blood cells contain both isoforms [5-8]. |
T1539 |
3467-3472 |
NN |
denotes |
Heart |
T1540 |
3473-3479 |
NN |
denotes |
muscle |
T1541 |
3514-3521 |
VBP |
denotes |
contain |
T1542 |
3479-3481 |
, |
denotes |
, |
T1543 |
3481-3486 |
NN |
denotes |
brain |
T1544 |
3487-3493 |
NN |
denotes |
tissue |
T1545 |
3494-3497 |
CC |
denotes |
and |
T1546 |
3498-3501 |
JJ |
denotes |
red |
T1547 |
3508-3513 |
NNS |
denotes |
cells |
T1548 |
3502-3507 |
NN |
denotes |
blood |
T1549 |
3522-3526 |
DT |
denotes |
both |
T1550 |
3527-3535 |
NNS |
denotes |
isoforms |
T1551 |
3536-3537 |
-LRB- |
denotes |
[ |
T1552 |
3537-3538 |
CD |
denotes |
5 |
T1553 |
3538-3539 |
SYM |
denotes |
- |
T1554 |
3539-3540 |
CD |
denotes |
8 |
T1555 |
3540-3541 |
-RRB- |
denotes |
] |
T1556 |
3541-3542 |
. |
denotes |
. |
T1557 |
3542-3658 |
sentence |
denotes |
Previous studies indicated that the subcellular localization of Annexin A7 changes during myoblast differentiation. |
T1558 |
3543-3551 |
JJ |
denotes |
Previous |
T1559 |
3552-3559 |
NNS |
denotes |
studies |
T1560 |
3560-3569 |
VBD |
denotes |
indicated |
T1561 |
3570-3574 |
IN |
denotes |
that |
T1562 |
3618-3625 |
VBZ |
denotes |
changes |
T1563 |
3575-3578 |
DT |
denotes |
the |
T1564 |
3591-3603 |
NN |
denotes |
localization |
T1565 |
3579-3590 |
JJ |
denotes |
subcellular |
T1566 |
3604-3606 |
IN |
denotes |
of |
T1567 |
3607-3614 |
NN |
denotes |
Annexin |
T1568 |
3615-3617 |
NN |
denotes |
A7 |
T1569 |
3626-3632 |
IN |
denotes |
during |
T1570 |
3633-3641 |
NN |
denotes |
myoblast |
T1571 |
3642-3657 |
NN |
denotes |
differentiation |
T1572 |
3657-3658 |
. |
denotes |
. |
T1573 |
3658-3844 |
sentence |
denotes |
In undifferentiated cells the protein is equally distributed between cytosol and membrane fractions while in differentiated cells it is exclusively present in the membrane fraction [7]. |
T1574 |
3659-3661 |
IN |
denotes |
In |
T1575 |
3708-3719 |
VBN |
denotes |
distributed |
T1576 |
3662-3678 |
JJ |
denotes |
undifferentiated |
T1577 |
3679-3684 |
NNS |
denotes |
cells |
T1578 |
3685-3688 |
DT |
denotes |
the |
T1579 |
3689-3696 |
NN |
denotes |
protein |
T1580 |
3697-3699 |
VBZ |
denotes |
is |
T1581 |
3700-3707 |
RB |
denotes |
equally |
T1582 |
3720-3727 |
IN |
denotes |
between |
T1583 |
3728-3735 |
NN |
denotes |
cytosol |
T1584 |
3749-3758 |
NNS |
denotes |
fractions |
T1585 |
3736-3739 |
CC |
denotes |
and |
T1586 |
3740-3748 |
NN |
denotes |
membrane |
T1587 |
3759-3764 |
IN |
denotes |
while |
T1588 |
3792-3794 |
VBZ |
denotes |
is |
T1589 |
3765-3767 |
IN |
denotes |
in |
T1590 |
3768-3782 |
VBN |
denotes |
differentiated |
T1591 |
3783-3788 |
NNS |
denotes |
cells |
T1592 |
3789-3791 |
PRP |
denotes |
it |
T1593 |
3795-3806 |
RB |
denotes |
exclusively |
T1594 |
3807-3814 |
JJ |
denotes |
present |
T1595 |
3815-3817 |
IN |
denotes |
in |
T1596 |
3818-3821 |
DT |
denotes |
the |
T1597 |
3831-3839 |
NN |
denotes |
fraction |
T1598 |
3822-3830 |
NN |
denotes |
membrane |
T1599 |
3840-3841 |
-LRB- |
denotes |
[ |
T1600 |
3841-3842 |
CD |
denotes |
7 |
T1601 |
3842-3843 |
-RRB- |
denotes |
] |
T1602 |
3843-3844 |
. |
denotes |
. |
T1603 |
3844-4069 |
sentence |
denotes |
Reports by Clemen et al. [9] and Herr et al. [8,10] demonstrated roles for Annexin A7 in shape and osmotic resistance of red blood cells, platelet aggregation velocity, and in the velocity of spreading astrocytic Ca2+-waves. |
T1604 |
3845-3852 |
NNS |
denotes |
Reports |
T1605 |
3897-3909 |
VBD |
denotes |
demonstrated |
T1606 |
3853-3855 |
IN |
denotes |
by |
T1607 |
3856-3862 |
NNP |
denotes |
Clemen |
T1608 |
3863-3865 |
FW |
denotes |
et |
T1609 |
3866-3869 |
FW |
denotes |
al. |
T1610 |
3870-3871 |
-LRB- |
denotes |
[ |
T1611 |
3871-3872 |
CD |
denotes |
9 |
T1612 |
3872-3873 |
-RRB- |
denotes |
] |
T1613 |
3874-3877 |
CC |
denotes |
and |
T1614 |
3878-3882 |
NNP |
denotes |
Herr |
T1615 |
3883-3885 |
FW |
denotes |
et |
T1616 |
3886-3889 |
FW |
denotes |
al. |
T1617 |
3890-3891 |
-LRB- |
denotes |
[ |
T1618 |
3893-3895 |
CD |
denotes |
10 |
T1619 |
3891-3892 |
CD |
denotes |
8 |
T1620 |
3892-3893 |
, |
denotes |
, |
T1621 |
3895-3896 |
-RRB- |
denotes |
] |
T1622 |
3910-3915 |
NNS |
denotes |
roles |
T1623 |
3916-3919 |
IN |
denotes |
for |
T1624 |
3920-3927 |
NN |
denotes |
Annexin |
T1625 |
3928-3930 |
NN |
denotes |
A7 |
T1626 |
3931-3933 |
IN |
denotes |
in |
T1627 |
3934-3939 |
NN |
denotes |
shape |
T1628 |
3940-3943 |
CC |
denotes |
and |
T1629 |
3944-3951 |
JJ |
denotes |
osmotic |
T1630 |
3952-3962 |
NN |
denotes |
resistance |
T1631 |
3963-3965 |
IN |
denotes |
of |
T1632 |
3966-3969 |
JJ |
denotes |
red |
T1633 |
3976-3981 |
NNS |
denotes |
cells |
T1634 |
3970-3975 |
NN |
denotes |
blood |
T1635 |
3981-3983 |
, |
denotes |
, |
T1636 |
3983-3991 |
NN |
denotes |
platelet |
T1637 |
4004-4012 |
NN |
denotes |
velocity |
T1638 |
3992-4003 |
NN |
denotes |
aggregation |
T1639 |
4012-4014 |
, |
denotes |
, |
T1640 |
4014-4017 |
CC |
denotes |
and |
T1641 |
4018-4020 |
IN |
denotes |
in |
T1642 |
4021-4024 |
DT |
denotes |
the |
T1643 |
4025-4033 |
NN |
denotes |
velocity |
T1644 |
4034-4036 |
IN |
denotes |
of |
T1645 |
4037-4046 |
VBG |
denotes |
spreading |
T1646 |
4063-4068 |
NNS |
denotes |
waves |
T1647 |
4047-4057 |
JJ |
denotes |
astrocytic |
T1648 |
4058-4062 |
NN |
denotes |
Ca2+ |
T1649 |
4062-4063 |
HYPH |
denotes |
- |
T1650 |
4068-4069 |
. |
denotes |
. |
T1651 |
4069-4205 |
sentence |
denotes |
Annexin A7 is also involved in the maintenance of regular cardiac electrophysiology and Ca2+-homeostasis [Schrickel et al., submitted]. |
T1652 |
4070-4077 |
NN |
denotes |
Annexin |
T1653 |
4078-4080 |
NN |
denotes |
A7 |
T1654 |
4089-4097 |
VBN |
denotes |
involved |
T1655 |
4081-4083 |
VBZ |
denotes |
is |
T1656 |
4084-4088 |
RB |
denotes |
also |
T1657 |
4098-4100 |
IN |
denotes |
in |
T1658 |
4101-4104 |
DT |
denotes |
the |
T1659 |
4105-4116 |
NN |
denotes |
maintenance |
T1660 |
4117-4119 |
IN |
denotes |
of |
T1661 |
4120-4127 |
JJ |
denotes |
regular |
T1662 |
4136-4153 |
NN |
denotes |
electrophysiology |
T1663 |
4128-4135 |
JJ |
denotes |
cardiac |
T1664 |
4154-4157 |
CC |
denotes |
and |
T1665 |
4158-4162 |
NN |
denotes |
Ca2+ |
T1666 |
4163-4174 |
NN |
denotes |
homeostasis |
T1667 |
4162-4163 |
HYPH |
denotes |
- |
T1668 |
4175-4176 |
-LRB- |
denotes |
[ |
T1669 |
4176-4185 |
NNP |
denotes |
Schrickel |
T1670 |
4186-4188 |
FW |
denotes |
et |
T1671 |
4189-4192 |
FW |
denotes |
al. |
T1672 |
4192-4194 |
, |
denotes |
, |
T1673 |
4194-4203 |
VBN |
denotes |
submitted |
T1674 |
4203-4204 |
-RRB- |
denotes |
] |
T1675 |
4204-4205 |
. |
denotes |
. |
T1676 |
4205-4311 |
sentence |
denotes |
Detailed reports on appearance and distribution of Annexin A7 during brain development are not available. |
T1677 |
4206-4214 |
JJ |
denotes |
Detailed |
T1678 |
4215-4222 |
NNS |
denotes |
reports |
T1679 |
4293-4296 |
VBP |
denotes |
are |
T1680 |
4223-4225 |
IN |
denotes |
on |
T1681 |
4226-4236 |
NN |
denotes |
appearance |
T1682 |
4237-4240 |
CC |
denotes |
and |
T1683 |
4241-4253 |
NN |
denotes |
distribution |
T1684 |
4254-4256 |
IN |
denotes |
of |
T1685 |
4257-4264 |
NN |
denotes |
Annexin |
T1686 |
4265-4267 |
NN |
denotes |
A7 |
T1687 |
4268-4274 |
IN |
denotes |
during |
T1688 |
4275-4280 |
NN |
denotes |
brain |
T1689 |
4281-4292 |
NN |
denotes |
development |
T1690 |
4297-4300 |
RB |
denotes |
not |
T1691 |
4301-4310 |
JJ |
denotes |
available |
T1692 |
4310-4311 |
. |
denotes |
. |
T1693 |
4311-4465 |
sentence |
denotes |
In the present study we focus on the distribution of Annexin A7 in the developing brain of mice embryos between E5 and E16, and in the adult mouse brain. |
T1694 |
4312-4314 |
IN |
denotes |
In |
T1695 |
4336-4341 |
VBP |
denotes |
focus |
T1696 |
4315-4318 |
DT |
denotes |
the |
T1697 |
4327-4332 |
NN |
denotes |
study |
T1698 |
4319-4326 |
JJ |
denotes |
present |
T1699 |
4333-4335 |
PRP |
denotes |
we |
T1700 |
4342-4344 |
IN |
denotes |
on |
T1701 |
4345-4348 |
DT |
denotes |
the |
T1702 |
4349-4361 |
NN |
denotes |
distribution |
T1703 |
4362-4364 |
IN |
denotes |
of |
T1704 |
4365-4372 |
NN |
denotes |
Annexin |
T1705 |
4373-4375 |
NN |
denotes |
A7 |
T1706 |
4376-4378 |
IN |
denotes |
in |
T1707 |
4379-4382 |
DT |
denotes |
the |
T1708 |
4394-4399 |
NN |
denotes |
brain |
T1709 |
4383-4393 |
VBG |
denotes |
developing |
T1710 |
4400-4402 |
IN |
denotes |
of |
T1711 |
4403-4407 |
NNS |
denotes |
mice |
T1712 |
4408-4415 |
NNS |
denotes |
embryos |
T1713 |
4416-4423 |
IN |
denotes |
between |
T1714 |
4424-4426 |
NN |
denotes |
E5 |
T1715 |
4427-4430 |
CC |
denotes |
and |
T1716 |
4431-4434 |
NN |
denotes |
E16 |
T1717 |
4434-4436 |
, |
denotes |
, |
T1718 |
4436-4439 |
CC |
denotes |
and |
T1719 |
4440-4442 |
IN |
denotes |
in |
T1720 |
4443-4446 |
DT |
denotes |
the |
T1721 |
4459-4464 |
NN |
denotes |
brain |
T1722 |
4447-4452 |
JJ |
denotes |
adult |
T1723 |
4453-4458 |
NN |
denotes |
mouse |
T1724 |
4464-4465 |
. |
denotes |
. |
T2289 |
4476-4483 |
NN |
denotes |
Annexin |
T2290 |
4484-4486 |
NN |
denotes |
A7 |
T2291 |
4490-4499 |
VBN |
denotes |
expressed |
T2292 |
4487-4489 |
VBZ |
denotes |
is |
T2293 |
4500-4502 |
IN |
denotes |
in |
T2294 |
4503-4506 |
DT |
denotes |
the |
T2295 |
4519-4525 |
NN |
denotes |
embryo |
T2296 |
4507-4512 |
JJ |
denotes |
early |
T2297 |
4513-4518 |
NN |
denotes |
mouse |
T2298 |
4525-4806 |
sentence |
denotes |
First we examined the expression of Annexin A7 in ES cells (Bruce4, established from C57BL/6J mice) and the early stages of mouse embryonic development at the mRNA level by northern blot analysis and at the protein level by Western blotting and immunohistochemistry, respectively. |
T2299 |
4526-4531 |
RB |
denotes |
First |
T2300 |
4535-4543 |
VBD |
denotes |
examined |
T2301 |
4532-4534 |
PRP |
denotes |
we |
T2302 |
4544-4547 |
DT |
denotes |
the |
T2303 |
4548-4558 |
NN |
denotes |
expression |
T2304 |
4559-4561 |
IN |
denotes |
of |
T2305 |
4562-4569 |
NN |
denotes |
Annexin |
T2306 |
4570-4572 |
NN |
denotes |
A7 |
T2307 |
4573-4575 |
IN |
denotes |
in |
T2308 |
4576-4578 |
NN |
denotes |
ES |
T2309 |
4579-4584 |
NNS |
denotes |
cells |
T2310 |
4585-4586 |
-LRB- |
denotes |
( |
T2311 |
4586-4592 |
NN |
denotes |
Bruce4 |
T2312 |
4592-4594 |
, |
denotes |
, |
T2313 |
4594-4605 |
VBN |
denotes |
established |
T2314 |
4606-4610 |
IN |
denotes |
from |
T2315 |
4611-4616 |
NN |
denotes |
C57BL |
T2316 |
4617-4619 |
NN |
denotes |
6J |
T2317 |
4616-4617 |
HYPH |
denotes |
/ |
T2318 |
4620-4624 |
NNS |
denotes |
mice |
T2319 |
4624-4625 |
-RRB- |
denotes |
) |
T2320 |
4626-4629 |
CC |
denotes |
and |
T2321 |
4630-4633 |
DT |
denotes |
the |
T2322 |
4640-4646 |
NNS |
denotes |
stages |
T2323 |
4634-4639 |
JJ |
denotes |
early |
T2324 |
4647-4649 |
IN |
denotes |
of |
T2325 |
4650-4655 |
NN |
denotes |
mouse |
T2326 |
4666-4677 |
NN |
denotes |
development |
T2327 |
4656-4665 |
JJ |
denotes |
embryonic |
T2328 |
4678-4680 |
IN |
denotes |
at |
T2329 |
4681-4684 |
DT |
denotes |
the |
T2330 |
4690-4695 |
NN |
denotes |
level |
T2331 |
4685-4689 |
NN |
denotes |
mRNA |
T2332 |
4696-4698 |
IN |
denotes |
by |
T2333 |
4699-4707 |
NNP |
denotes |
northern |
T2334 |
4708-4712 |
NN |
denotes |
blot |
T2335 |
4713-4721 |
NN |
denotes |
analysis |
T2336 |
4722-4725 |
CC |
denotes |
and |
T2337 |
4726-4728 |
IN |
denotes |
at |
T2338 |
4729-4732 |
DT |
denotes |
the |
T2339 |
4741-4746 |
NN |
denotes |
level |
T2340 |
4733-4740 |
NN |
denotes |
protein |
T2341 |
4747-4749 |
IN |
denotes |
by |
T2342 |
4750-4757 |
NNP |
denotes |
Western |
T2343 |
4758-4766 |
NN |
denotes |
blotting |
T2344 |
4767-4770 |
CC |
denotes |
and |
T2345 |
4771-4791 |
NN |
denotes |
immunohistochemistry |
T2346 |
4791-4793 |
, |
denotes |
, |
T2347 |
4793-4805 |
RB |
denotes |
respectively |
T2348 |
4805-4806 |
. |
denotes |
. |
T2349 |
4806-5011 |
sentence |
denotes |
Northern blot analysis shows in ES-cells and at embryonic days E7, E11, E15, and E17 two mRNA species of 1.8 kb and 2.4 kb, which result from alternative splicing in the untranslated 3'end (Fig. 1A) [11]. |
T2350 |
4807-4815 |
NNP |
denotes |
Northern |
T2351 |
4816-4820 |
NN |
denotes |
blot |
T2352 |
4821-4829 |
NN |
denotes |
analysis |
T2353 |
4830-4835 |
VBZ |
denotes |
shows |
T2354 |
4836-4838 |
IN |
denotes |
in |
T2355 |
4839-4841 |
NN |
denotes |
ES |
T2356 |
4842-4847 |
NNS |
denotes |
cells |
T2357 |
4841-4842 |
HYPH |
denotes |
- |
T2358 |
4848-4851 |
CC |
denotes |
and |
T2359 |
4852-4854 |
IN |
denotes |
at |
T2360 |
4855-4864 |
JJ |
denotes |
embryonic |
T2361 |
4870-4872 |
NN |
denotes |
E7 |
T2362 |
4865-4869 |
NNS |
denotes |
days |
T2363 |
4872-4874 |
, |
denotes |
, |
T2364 |
4874-4877 |
NN |
denotes |
E11 |
T2365 |
4877-4879 |
, |
denotes |
, |
T2366 |
4879-4882 |
NN |
denotes |
E15 |
T2367 |
4882-4884 |
, |
denotes |
, |
T2368 |
4884-4887 |
CC |
denotes |
and |
T2369 |
4888-4891 |
NN |
denotes |
E17 |
T2370 |
4892-4895 |
CD |
denotes |
two |
T2371 |
4901-4908 |
NNS |
denotes |
species |
T2372 |
4896-4900 |
NN |
denotes |
mRNA |
T2373 |
4909-4911 |
IN |
denotes |
of |
T2374 |
4912-4915 |
CD |
denotes |
1.8 |
T2375 |
4916-4918 |
NN |
denotes |
kb |
T2376 |
4919-4922 |
CC |
denotes |
and |
T2377 |
4923-4926 |
CD |
denotes |
2.4 |
T2378 |
4927-4929 |
NN |
denotes |
kb |
T2379 |
4929-4931 |
, |
denotes |
, |
T2380 |
4931-4936 |
WDT |
denotes |
which |
T2381 |
4937-4943 |
VBP |
denotes |
result |
T2382 |
4944-4948 |
IN |
denotes |
from |
T2383 |
4949-4960 |
JJ |
denotes |
alternative |
T2384 |
4961-4969 |
NN |
denotes |
splicing |
T2385 |
4970-4972 |
IN |
denotes |
in |
T2386 |
4973-4976 |
DT |
denotes |
the |
T2387 |
4992-4995 |
NN |
denotes |
end |
T2388 |
4977-4989 |
JJ |
denotes |
untranslated |
T2389 |
4990-4991 |
CD |
denotes |
3 |
T2390 |
4991-4992 |
SYM |
denotes |
' |
T2391 |
4996-4997 |
-LRB- |
denotes |
( |
T2392 |
4997-5001 |
NN |
denotes |
Fig. |
T2393 |
5002-5004 |
CD |
denotes |
1A |
T2394 |
5004-5005 |
-RRB- |
denotes |
) |
T2395 |
5006-5007 |
-LRB- |
denotes |
[ |
T2396 |
5007-5009 |
CD |
denotes |
11 |
T2397 |
5009-5010 |
-RRB- |
denotes |
] |
T2398 |
5010-5011 |
. |
denotes |
. |
T2399 |
5011-5081 |
sentence |
denotes |
We found similar Annexin A7 mRNA levels in the four embryonic stages. |
T2400 |
5012-5014 |
PRP |
denotes |
We |
T2401 |
5015-5020 |
VBD |
denotes |
found |
T2402 |
5021-5028 |
JJ |
denotes |
similar |
T2426 |
5137-5140 |
DT |
denotes |
the |
T2427 |
5141-5151 |
NN |
denotes |
appearance |
T2428 |
5152-5154 |
IN |
denotes |
of |
T2429 |
5155-5156 |
DT |
denotes |
a |
T2430 |
5179-5186 |
NNS |
denotes |
species |
T2431 |
5157-5163 |
JJR |
denotes |
faster |
T2432 |
5164-5173 |
VBG |
denotes |
migrating |
T2433 |
5174-5178 |
NN |
denotes |
mRNA |
T2434 |
5187-5189 |
IN |
denotes |
in |
T2435 |
5190-5198 |
NN |
denotes |
addition |
T2436 |
5199-5201 |
IN |
denotes |
to |
T2437 |
5202-5205 |
DT |
denotes |
the |
T2438 |
5213-5220 |
NNS |
denotes |
species |
T2439 |
5206-5209 |
CD |
denotes |
2.0 |
T2440 |
5210-5212 |
NN |
denotes |
kb |
T2441 |
5224-5238 |
JJ |
denotes |
characteristic |
T2442 |
5239-5242 |
IN |
denotes |
for |
T2443 |
5243-5244 |
NN |
denotes |
β |
T2444 |
5245-5250 |
NN |
denotes |
actin |
T2445 |
5244-5245 |
HYPH |
denotes |
- |
T2446 |
5251-5252 |
-LRB- |
denotes |
[ |
T2447 |
5252-5254 |
CD |
denotes |
12 |
T2448 |
5254-5255 |
-RRB- |
denotes |
] |
T2449 |
5255-5256 |
. |
denotes |
. |
T2450 |
5256-5410 |
sentence |
denotes |
On the protein level, ES cells express only the smaller Annexin A7 isoform of 47 kDa (Fig. 1B, neuro-2a cells and heart tissue are included for control). |
T2451 |
5257-5259 |
IN |
denotes |
On |
T2452 |
5288-5295 |
VBP |
denotes |
express |
T2453 |
5260-5263 |
DT |
denotes |
the |
T2454 |
5272-5277 |
NN |
denotes |
level |
T2455 |
5264-5271 |
NN |
denotes |
protein |
T2456 |
5277-5279 |
, |
denotes |
, |
T2457 |
5279-5281 |
NN |
denotes |
ES |
T2458 |
5282-5287 |
NNS |
denotes |
cells |
T2459 |
5296-5300 |
RB |
denotes |
only |
T2460 |
5324-5331 |
NN |
denotes |
isoform |
T2461 |
5301-5304 |
DT |
denotes |
the |
T2462 |
5305-5312 |
JJR |
denotes |
smaller |
T2463 |
5313-5320 |
NN |
denotes |
Annexin |
T2464 |
5321-5323 |
NN |
denotes |
A7 |
T2465 |
5332-5334 |
IN |
denotes |
of |
T2466 |
5335-5337 |
CD |
denotes |
47 |
T2467 |
5338-5341 |
NN |
denotes |
kDa |
T2468 |
5342-5343 |
-LRB- |
denotes |
( |
T2469 |
5348-5350 |
NN |
denotes |
1B |
T2470 |
5343-5347 |
NN |
denotes |
Fig. |
T2471 |
5350-5352 |
, |
denotes |
, |
T2472 |
5352-5357 |
NN |
denotes |
neuro |
T2473 |
5361-5366 |
NNS |
denotes |
cells |
T2474 |
5357-5358 |
HYPH |
denotes |
- |
T2475 |
5358-5360 |
CD |
denotes |
2a |
T2476 |
5388-5396 |
VBN |
denotes |
included |
T2477 |
5367-5370 |
CC |
denotes |
and |
T2478 |
5371-5376 |
NN |
denotes |
heart |
T2479 |
5377-5383 |
NN |
denotes |
tissue |
T2480 |
5384-5387 |
VBP |
denotes |
are |
T2481 |
5397-5400 |
IN |
denotes |
for |
T2482 |
5401-5408 |
NN |
denotes |
control |
T2483 |
5408-5409 |
-RRB- |
denotes |
) |
T2484 |
5409-5410 |
. |
denotes |
. |
T2535 |
6529-6531 |
NN |
denotes |
A7 |
T2536 |
6543-6545 |
IN |
denotes |
in |
T2537 |
6546-6549 |
DT |
denotes |
the |
T2538 |
6550-6558 |
NN |
denotes |
ectoderm |
T2539 |
6559-6560 |
-LRB- |
denotes |
( |
T2540 |
6565-6567 |
NN |
denotes |
2A |
T2541 |
6560-6564 |
NN |
denotes |
Fig. |
T2542 |
6567-6568 |
SYM |
denotes |
– |
T2543 |
6568-6569 |
NN |
denotes |
C |
T2544 |
6569-6570 |
-RRB- |
denotes |
) |
T2545 |
6570-6571 |
. |
denotes |
. |
T2546 |
6571-6658 |
sentence |
denotes |
At E8 Annexin A7 can be detected in almost all tissues of the embryo (data not shown). |
T2547 |
6572-6574 |
IN |
denotes |
At |
T2548 |
6596-6604 |
VBN |
denotes |
detected |
T2549 |
6575-6577 |
NN |
denotes |
E8 |
T2550 |
6578-6585 |
NN |
denotes |
Annexin |
T2551 |
6586-6588 |
NN |
denotes |
A7 |
T2552 |
6589-6592 |
MD |
denotes |
can |
T2553 |
6593-6595 |
VB |
denotes |
be |
T2554 |
6605-6607 |
IN |
denotes |
in |
T2555 |
6608-6614 |
RB |
denotes |
almost |
T2556 |
6619-6626 |
NNS |
denotes |
tissues |
T2557 |
6615-6618 |
DT |
denotes |
all |
T2558 |
6627-6629 |
IN |
denotes |
of |
T2559 |
6630-6633 |
DT |
denotes |
the |
T2560 |
6634-6640 |
NN |
denotes |
embryo |
T2561 |
6641-6642 |
-LRB- |
denotes |
( |
T2562 |
6651-6656 |
VBN |
denotes |
shown |
T2563 |
6642-6646 |
NNS |
denotes |
data |
T2564 |
6647-6650 |
RB |
denotes |
not |
T2565 |
6656-6657 |
-RRB- |
denotes |
) |
T2566 |
6657-6658 |
. |
denotes |
. |
T2567 |
6658-6788 |
sentence |
denotes |
A correlation between the presence of Annexin A7 and the origin of a cell type from one of the three germ layers is not apparent. |
T2568 |
6659-6660 |
DT |
denotes |
A |
T2569 |
6661-6672 |
NN |
denotes |
correlation |
T2570 |
6772-6774 |
VBZ |
denotes |
is |
T2571 |
6673-6680 |
IN |
denotes |
between |
T2572 |
6681-6684 |
DT |
denotes |
the |
T2573 |
6685-6693 |
NN |
denotes |
presence |
T2574 |
6694-6696 |
IN |
denotes |
of |
T2575 |
6697-6704 |
NN |
denotes |
Annexin |
T2576 |
6705-6707 |
NN |
denotes |
A7 |
T2577 |
6708-6711 |
CC |
denotes |
and |
T2578 |
6712-6715 |
DT |
denotes |
the |
T2579 |
6716-6722 |
NN |
denotes |
origin |
T2580 |
6723-6725 |
IN |
denotes |
of |
T2581 |
6726-6727 |
DT |
denotes |
a |
T2582 |
6733-6737 |
NN |
denotes |
type |
T2583 |
6728-6732 |
NN |
denotes |
cell |
T2584 |
6738-6742 |
IN |
denotes |
from |
T2585 |
6743-6746 |
CD |
denotes |
one |
T2586 |
6754-6759 |
CD |
denotes |
three |
T2587 |
6747-6749 |
IN |
denotes |
of |
T2588 |
6750-6753 |
DT |
denotes |
the |
T2589 |
6765-6771 |
NNS |
denotes |
layers |
T2590 |
6760-6764 |
NN |
denotes |
germ |
T2591 |
6775-6778 |
RB |
denotes |
not |
T2592 |
6779-6787 |
JJ |
denotes |
apparent |
T2593 |
6787-6788 |
. |
denotes |
. |
T2594 |
6788-6944 |
sentence |
denotes |
We specifically noted the localization of the protein in the neuroepithelium of the neural fold and tube (Fig. 2D–F, proximal, E8; Fig. 2G–I, distal, E13). |
T2595 |
6789-6791 |
PRP |
denotes |
We |
T2596 |
6805-6810 |
VBD |
denotes |
noted |
T2597 |
6792-6804 |
RB |
denotes |
specifically |
T2598 |
6811-6814 |
DT |
denotes |
the |
T2599 |
6815-6827 |
NN |
denotes |
localization |
T2600 |
6828-6830 |
IN |
denotes |
of |
T2601 |
6831-6834 |
DT |
denotes |
the |
T2602 |
6835-6842 |
NN |
denotes |
protein |
T2603 |
6843-6845 |
IN |
denotes |
in |
T2604 |
6846-6849 |
DT |
denotes |
the |
T2605 |
6850-6865 |
NN |
denotes |
neuroepithelium |
T2606 |
6866-6868 |
IN |
denotes |
of |
T2607 |
6869-6872 |
DT |
denotes |
the |
T2608 |
6880-6884 |
NN |
denotes |
fold |
T2609 |
6873-6879 |
JJ |
denotes |
neural |
T2610 |
6885-6888 |
CC |
denotes |
and |
T2611 |
6889-6893 |
NN |
denotes |
tube |
T2612 |
6894-6895 |
-LRB- |
denotes |
( |
T2613 |
6939-6942 |
NN |
denotes |
E13 |
T2614 |
6895-6899 |
NN |
denotes |
Fig. |
T2615 |
6900-6902 |
NN |
denotes |
2D |
T2616 |
6916-6918 |
NN |
denotes |
E8 |
T2617 |
6902-6903 |
SYM |
denotes |
– |
T2618 |
6903-6904 |
NN |
denotes |
F |
T2619 |
6904-6906 |
, |
denotes |
, |
T2620 |
6906-6914 |
JJ |
denotes |
proximal |
T2621 |
6914-6916 |
, |
denotes |
, |
T2622 |
6918-6919 |
: |
denotes |
; |
T2623 |
6920-6924 |
NN |
denotes |
Fig. |
T2624 |
6925-6927 |
NN |
denotes |
2G |
T2625 |
6927-6928 |
SYM |
denotes |
– |
T2626 |
6928-6929 |
NN |
denotes |
I |
T2627 |
6929-6931 |
, |
denotes |
, |
T2628 |
6931-6937 |
JJ |
denotes |
distal |
T2629 |
6937-6939 |
, |
denotes |
, |
T2630 |
6942-6943 |
-RRB- |
denotes |
) |
T2631 |
6943-6944 |
. |
denotes |
. |
T2632 |
6944-7073 |
sentence |
denotes |
At the subcellular level, Annexin A7 was present in punctate structures mainly in the cytosol of all examined cells (Fig. 2E,F). |
T2633 |
6945-6947 |
IN |
denotes |
At |
T2634 |
6982-6985 |
VBD |
denotes |
was |
T2635 |
6948-6951 |
DT |
denotes |
the |
T2636 |
6964-6969 |
NN |
denotes |
level |
T2637 |
6952-6963 |
JJ |
denotes |
subcellular |
T2638 |
6969-6971 |
, |
denotes |
, |
T2639 |
6971-6978 |
NN |
denotes |
Annexin |
T2640 |
6979-6981 |
NN |
denotes |
A7 |
T2641 |
6986-6993 |
JJ |
denotes |
present |
T2642 |
6994-6996 |
IN |
denotes |
in |
T2643 |
6997-7005 |
NN |
denotes |
punctate |
T2644 |
7006-7016 |
NNS |
denotes |
structures |
T2645 |
7017-7023 |
RB |
denotes |
mainly |
T2646 |
7024-7026 |
IN |
denotes |
in |
T2647 |
7027-7030 |
DT |
denotes |
the |
T2648 |
7031-7038 |
NN |
denotes |
cytosol |
T2649 |
7039-7041 |
IN |
denotes |
of |
T2650 |
7042-7045 |
DT |
denotes |
all |
T2651 |
7055-7060 |
NNS |
denotes |
cells |
T2652 |
7046-7054 |
VBN |
denotes |
examined |
T2653 |
7061-7062 |
-LRB- |
denotes |
( |
T2654 |
7070-7071 |
NN |
denotes |
F |
T2655 |
7062-7066 |
NN |
denotes |
Fig. |
T2656 |
7067-7069 |
NN |
denotes |
2E |
T2657 |
7069-7070 |
, |
denotes |
, |
T2658 |
7071-7072 |
-RRB- |
denotes |
) |
T2659 |
7072-7073 |
. |
denotes |
. |
T2660 |
7073-7140 |
sentence |
denotes |
This distribution was also observed during subsequent development. |
T2661 |
7074-7078 |
DT |
denotes |
This |
T2662 |
7079-7091 |
NN |
denotes |
distribution |
T2663 |
7101-7109 |
VBN |
denotes |
observed |
T2664 |
7092-7095 |
VBD |
denotes |
was |
T2665 |
7096-7100 |
RB |
denotes |
also |
T2666 |
7110-7116 |
IN |
denotes |
during |
T2667 |
7117-7127 |
JJ |
denotes |
subsequent |
T2668 |
7128-7139 |
NN |
denotes |
development |
T2669 |
7139-7140 |
. |
denotes |
. |
T2973 |
8537-8544 |
NN |
denotes |
Annexin |
T2974 |
8545-8547 |
NN |
denotes |
A7 |
T2975 |
8548-8555 |
VBZ |
denotes |
changes |
T2976 |
8556-8559 |
PRP$ |
denotes |
its |
T2977 |
8572-8584 |
NN |
denotes |
localization |
T2978 |
8560-8571 |
JJ |
denotes |
subcellular |
T2979 |
8585-8587 |
IN |
denotes |
in |
T2980 |
8588-8591 |
DT |
denotes |
the |
T2981 |
8602-8607 |
NN |
denotes |
brain |
T2982 |
8592-8601 |
JJ |
denotes |
embryonic |
T2983 |
8608-8610 |
IN |
denotes |
at |
T2984 |
8611-8614 |
NN |
denotes |
E16 |
T2985 |
8614-8727 |
sentence |
denotes |
Next we analysed the cellular and subcellular distribution of Annexin A7 in the developing mouse brain (Fig. 3). |
T2986 |
8615-8619 |
RB |
denotes |
Next |
T2987 |
8623-8631 |
VBD |
denotes |
analysed |
T2988 |
8620-8622 |
PRP |
denotes |
we |
T2989 |
8632-8635 |
DT |
denotes |
the |
T2990 |
8661-8673 |
NN |
denotes |
distribution |
T2991 |
8636-8644 |
JJ |
denotes |
cellular |
T2992 |
8645-8648 |
CC |
denotes |
and |
T2993 |
8649-8660 |
JJ |
denotes |
subcellular |
T2994 |
8674-8676 |
IN |
denotes |
of |
T2995 |
8677-8684 |
NN |
denotes |
Annexin |
T2996 |
8685-8687 |
NN |
denotes |
A7 |
T2997 |
8688-8690 |
IN |
denotes |
in |
T2998 |
8691-8694 |
DT |
denotes |
the |
T2999 |
8712-8717 |
NN |
denotes |
brain |
T3000 |
8695-8705 |
VBG |
denotes |
developing |
T3001 |
8706-8711 |
NN |
denotes |
mouse |
T3002 |
8718-8719 |
-LRB- |
denotes |
( |
T3003 |
8719-8723 |
NN |
denotes |
Fig. |
T3004 |
8724-8725 |
CD |
denotes |
3 |
T3005 |
8725-8726 |
-RRB- |
denotes |
) |
T3006 |
8726-8727 |
. |
denotes |
. |
T3007 |
8727-8948 |
sentence |
denotes |
At E13–E16 in transverse sections of the embryonic brain, most of the immunoreactive cells are present in the ventricular germinative zone surrounding the lateral ventricle (Fig. 3A, overview E16; Fig. 3H, overview E13). |
T3008 |
8728-8730 |
IN |
denotes |
At |
T3009 |
8819-8822 |
VBP |
denotes |
are |
T3010 |
8731-8734 |
NN |
denotes |
E13 |
T3011 |
8734-8735 |
SYM |
denotes |
– |
T3012 |
8735-8738 |
NN |
denotes |
E16 |
T3013 |
8739-8741 |
IN |
denotes |
in |
T3014 |
8742-8752 |
JJ |
denotes |
transverse |
T3015 |
8753-8761 |
NNS |
denotes |
sections |
T3016 |
8762-8764 |
IN |
denotes |
of |
T3017 |
8765-8768 |
DT |
denotes |
the |
T3018 |
8779-8784 |
NN |
denotes |
brain |
T3019 |
8769-8778 |
JJ |
denotes |
embryonic |
T3020 |
8784-8786 |
, |
denotes |
, |
T3021 |
8786-8790 |
JJS |
denotes |
most |
T3022 |
8791-8793 |
IN |
denotes |
of |
T3023 |
8794-8797 |
DT |
denotes |
the |
T3024 |
8813-8818 |
NNS |
denotes |
cells |
T3025 |
8798-8812 |
JJ |
denotes |
immunoreactive |
T3026 |
8823-8830 |
JJ |
denotes |
present |
T3027 |
8831-8833 |
IN |
denotes |
in |
T3028 |
8834-8837 |
DT |
denotes |
the |
T3029 |
8862-8866 |
NN |
denotes |
zone |
T3030 |
8838-8849 |
JJ |
denotes |
ventricular |
T3031 |
8850-8861 |
JJ |
denotes |
germinative |
T3032 |
8867-8878 |
VBG |
denotes |
surrounding |
T3033 |
8879-8882 |
DT |
denotes |
the |
T3034 |
8891-8900 |
NN |
denotes |
ventricle |
T3035 |
8883-8890 |
JJ |
denotes |
lateral |
T3036 |
8901-8902 |
-LRB- |
denotes |
( |
T3037 |
8943-8946 |
NN |
denotes |
E13 |
T3038 |
8902-8906 |
NN |
denotes |
Fig. |
T3039 |
8920-8923 |
NN |
denotes |
E16 |
T3040 |
8907-8909 |
CD |
denotes |
3A |
T3041 |
8909-8911 |
, |
denotes |
, |
T3042 |
8911-8919 |
NN |
denotes |
overview |
T3043 |
8923-8924 |
: |
denotes |
; |
T3044 |
8925-8929 |
NN |
denotes |
Fig. |
T3045 |
8930-8932 |
CD |
denotes |
3H |
T3046 |
8932-8934 |
, |
denotes |
, |
T3047 |
8934-8942 |
NN |
denotes |
overview |
T3048 |
8946-8947 |
-RRB- |
denotes |
) |
T3049 |
8947-8948 |
. |
denotes |
. |
T3050 |
8948-9048 |
sentence |
denotes |
A closer inspection revealed that Annexin A7 is mainly localized in the cytosol at E13 (Fig. 3B,E). |
T3051 |
8949-8950 |
DT |
denotes |
A |
T3052 |
8958-8968 |
NN |
denotes |
inspection |
T3053 |
8951-8957 |
JJR |
denotes |
closer |
T3054 |
8969-8977 |
VBD |
denotes |
revealed |
T3055 |
8978-8982 |
IN |
denotes |
that |
T3056 |
9004-9013 |
VBN |
denotes |
localized |
T3057 |
8983-8990 |
NN |
denotes |
Annexin |
T3058 |
8991-8993 |
NN |
denotes |
A7 |
T3059 |
8994-8996 |
VBZ |
denotes |
is |
T3060 |
8997-9003 |
RB |
denotes |
mainly |
T3061 |
9014-9016 |
IN |
denotes |
in |
T3062 |
9017-9020 |
DT |
denotes |
the |
T3063 |
9021-9028 |
NN |
denotes |
cytosol |
T3064 |
9029-9031 |
IN |
denotes |
at |
T3065 |
9032-9035 |
NN |
denotes |
E13 |
T3066 |
9036-9037 |
-LRB- |
denotes |
( |
T3067 |
9045-9046 |
NN |
denotes |
E |
T3068 |
9037-9041 |
NN |
denotes |
Fig. |
T3069 |
9042-9044 |
CD |
denotes |
3B |
T3070 |
9044-9045 |
, |
denotes |
, |
T3071 |
9046-9047 |
-RRB- |
denotes |
) |
T3072 |
9047-9048 |
. |
denotes |
. |
T3073 |
9048-9188 |
sentence |
denotes |
During further development the immature cells have rounded up two days later, but they retain Annexin A7 in the cytosol at E15 (Fig. 3C,F). |
T3074 |
9049-9055 |
IN |
denotes |
During |
T3075 |
9100-9107 |
VBN |
denotes |
rounded |
T3076 |
9056-9063 |
JJ |
denotes |
further |
T3077 |
9064-9075 |
NN |
denotes |
development |
T3078 |
9076-9079 |
DT |
denotes |
the |
T3079 |
9089-9094 |
NNS |
denotes |
cells |
T3080 |
9080-9088 |
JJ |
denotes |
immature |
T3081 |
9095-9099 |
VBP |
denotes |
have |
T3082 |
9108-9110 |
RP |
denotes |
up |
T3083 |
9111-9114 |
CD |
denotes |
two |
T3084 |
9115-9119 |
NNS |
denotes |
days |
T3085 |
9120-9125 |
RB |
denotes |
later |
T3086 |
9125-9127 |
, |
denotes |
, |
T3087 |
9127-9130 |
CC |
denotes |
but |
T3088 |
9131-9135 |
PRP |
denotes |
they |
T3089 |
9136-9142 |
VBP |
denotes |
retain |
T3090 |
9143-9150 |
NN |
denotes |
Annexin |
T3091 |
9151-9153 |
NN |
denotes |
A7 |
T3092 |
9154-9156 |
IN |
denotes |
in |
T3093 |
9157-9160 |
DT |
denotes |
the |
T3094 |
9161-9168 |
NN |
denotes |
cytosol |
T3095 |
9169-9171 |
IN |
denotes |
at |
T3096 |
9172-9175 |
NN |
denotes |
E15 |
T3097 |
9176-9177 |
-LRB- |
denotes |
( |
T3098 |
9185-9186 |
NN |
denotes |
F |
T3099 |
9177-9181 |
NN |
denotes |
Fig. |
T3100 |
9182-9184 |
CD |
denotes |
3C |
T3101 |
9184-9185 |
, |
denotes |
, |
T3102 |
9186-9187 |
-RRB- |
denotes |
) |
T3103 |
9187-9188 |
. |
denotes |
. |
T3104 |
9188-9476 |
sentence |
denotes |
At E16 we observed the first prominent nuclear staining of Annexin A7 (Fig. 3D,G) in cells of the intermediate zone (Fig. 3I, oval), which contains neurons radially migrating towards the growing neopallium cortex [13,14] and also glial cells forming the white matter of the adult cortex. |
T3105 |
9189-9191 |
IN |
denotes |
At |
T3106 |
9199-9207 |
VBD |
denotes |
observed |
T3107 |
9192-9195 |
NN |
denotes |
E16 |
T3108 |
9196-9198 |
PRP |
denotes |
we |
T3109 |
9208-9211 |
DT |
denotes |
the |
T3110 |
9236-9244 |
NN |
denotes |
staining |
T3111 |
9212-9217 |
JJ |
denotes |
first |
T3112 |
9218-9227 |
JJ |
denotes |
prominent |
T3113 |
9228-9235 |
JJ |
denotes |
nuclear |
T3114 |
9245-9247 |
IN |
denotes |
of |
T3115 |
9248-9255 |
NN |
denotes |
Annexin |
T3116 |
9256-9258 |
NN |
denotes |
A7 |
T3117 |
9259-9260 |
-LRB- |
denotes |
( |
T3118 |
9268-9269 |
NN |
denotes |
G |
T3119 |
9260-9264 |
NN |
denotes |
Fig. |
T3120 |
9265-9267 |
NN |
denotes |
3D |
T3121 |
9267-9268 |
, |
denotes |
, |
T3122 |
9269-9270 |
-RRB- |
denotes |
) |
T3123 |
9271-9273 |
IN |
denotes |
in |
T3124 |
9274-9279 |
NNS |
denotes |
cells |
T3125 |
9280-9282 |
IN |
denotes |
of |
T3126 |
9283-9286 |
DT |
denotes |
the |
T3127 |
9300-9304 |
NN |
denotes |
zone |
T3128 |
9287-9299 |
JJ |
denotes |
intermediate |
T3129 |
9305-9306 |
-LRB- |
denotes |
( |
T3130 |
9315-9319 |
NN |
denotes |
oval |
T3131 |
9306-9310 |
NN |
denotes |
Fig. |
T3132 |
9311-9313 |
NN |
denotes |
3I |
T3133 |
9313-9315 |
, |
denotes |
, |
T3134 |
9319-9320 |
-RRB- |
denotes |
) |
T3135 |
9320-9322 |
, |
denotes |
, |
T3136 |
9322-9327 |
WDT |
denotes |
which |
T3137 |
9328-9336 |
VBZ |
denotes |
contains |
T3138 |
9337-9344 |
NNS |
denotes |
neurons |
T3139 |
9345-9353 |
RB |
denotes |
radially |
T3140 |
9354-9363 |
VBG |
denotes |
migrating |
T3141 |
9364-9371 |
IN |
denotes |
towards |
T3142 |
9372-9375 |
DT |
denotes |
the |
T3143 |
9395-9401 |
NN |
denotes |
cortex |
T3144 |
9376-9383 |
VBG |
denotes |
growing |
T3145 |
9384-9394 |
NN |
denotes |
neopallium |
T3146 |
9402-9403 |
-LRB- |
denotes |
[ |
T3147 |
9406-9408 |
CD |
denotes |
14 |
T3148 |
9403-9405 |
CD |
denotes |
13 |
T3149 |
9405-9406 |
, |
denotes |
, |
T3150 |
9408-9409 |
-RRB- |
denotes |
] |
T3151 |
9410-9413 |
CC |
denotes |
and |
T3152 |
9414-9418 |
RB |
denotes |
also |
T3153 |
9419-9424 |
JJ |
denotes |
glial |
T3154 |
9425-9430 |
NNS |
denotes |
cells |
T3155 |
9431-9438 |
VBG |
denotes |
forming |
T3156 |
9439-9442 |
DT |
denotes |
the |
T3157 |
9449-9455 |
NN |
denotes |
matter |
T3158 |
9443-9448 |
JJ |
denotes |
white |
T3159 |
9456-9458 |
IN |
denotes |
of |
T3160 |
9459-9462 |
DT |
denotes |
the |
T3161 |
9469-9475 |
NN |
denotes |
cortex |
T3162 |
9463-9468 |
JJ |
denotes |
adult |
T3163 |
9475-9476 |
. |
denotes |
. |
T3164 |
9476-9556 |
sentence |
denotes |
Cells located marginally in the neopallial cortex also exhibit a nuclear stain. |
T3165 |
9477-9482 |
NNS |
denotes |
Cells |
T3166 |
9532-9539 |
VBP |
denotes |
exhibit |
T3167 |
9483-9490 |
VBN |
denotes |
located |
T3168 |
9491-9501 |
RB |
denotes |
marginally |
T3169 |
9502-9504 |
IN |
denotes |
in |
T3170 |
9505-9508 |
DT |
denotes |
the |
T3171 |
9520-9526 |
NN |
denotes |
cortex |
T3172 |
9509-9519 |
JJ |
denotes |
neopallial |
T3173 |
9527-9531 |
RB |
denotes |
also |
T3174 |
9540-9541 |
DT |
denotes |
a |
T3175 |
9550-9555 |
NN |
denotes |
stain |
T3176 |
9542-9549 |
JJ |
denotes |
nuclear |
T3177 |
9555-9556 |
. |
denotes |
. |
T3178 |
9556-9689 |
sentence |
denotes |
Labelling in the ventricular germinative zone is less prominent and the cytosol is no longer more strongly stained than the nucleus. |
T3179 |
9557-9566 |
NN |
denotes |
Labelling |
T3180 |
9603-9605 |
VBZ |
denotes |
is |
T3181 |
9567-9569 |
IN |
denotes |
in |
T3182 |
9570-9573 |
DT |
denotes |
the |
T3183 |
9598-9602 |
NN |
denotes |
zone |
T3184 |
9574-9585 |
JJ |
denotes |
ventricular |
T3185 |
9586-9597 |
JJ |
denotes |
germinative |
T3186 |
9606-9610 |
RBR |
denotes |
less |
T3187 |
9611-9620 |
JJ |
denotes |
prominent |
T3188 |
9621-9624 |
CC |
denotes |
and |
T3189 |
9625-9628 |
DT |
denotes |
the |
T3190 |
9629-9636 |
NN |
denotes |
cytosol |
T3191 |
9637-9639 |
VBZ |
denotes |
is |
T3192 |
9640-9642 |
RB |
denotes |
no |
T3193 |
9643-9649 |
RB |
denotes |
longer |
T3194 |
9650-9654 |
RBR |
denotes |
more |
T3195 |
9655-9663 |
RB |
denotes |
strongly |
T3196 |
9664-9671 |
VBN |
denotes |
stained |
T3197 |
9672-9676 |
IN |
denotes |
than |
T3198 |
9677-9680 |
DT |
denotes |
the |
T3199 |
9681-9688 |
NN |
denotes |
nucleus |
T3200 |
9688-9689 |
. |
denotes |
. |
T3569 |
10879-10885 |
JJ |
denotes |
Mature |
T3570 |
10886-10893 |
NNS |
denotes |
neurons |
T3571 |
10915-10919 |
VBP |
denotes |
show |
T3572 |
10894-10896 |
IN |
denotes |
in |
T3573 |
10897-10902 |
JJ |
denotes |
adult |
T3574 |
10909-10914 |
NN |
denotes |
brain |
T3575 |
10903-10908 |
NN |
denotes |
mouse |
T3576 |
10920-10922 |
DT |
denotes |
an |
T3577 |
10939-10947 |
NN |
denotes |
staining |
T3578 |
10923-10930 |
JJ |
denotes |
intense |
T3579 |
10931-10938 |
JJ |
denotes |
nuclear |
T3580 |
10948-10950 |
IN |
denotes |
of |
T3581 |
10951-10958 |
NN |
denotes |
Annexin |
T3582 |
10959-10961 |
NN |
denotes |
A7 |
T3583 |
10961-11029 |
sentence |
denotes |
Both Annexin A7 isoforms are found in adult brain tissue (Fig. 1B). |
T3584 |
10962-10966 |
CC |
denotes |
Both |
T3585 |
10978-10986 |
NNS |
denotes |
isoforms |
T3586 |
10967-10974 |
NN |
denotes |
Annexin |
T3587 |
10975-10977 |
NN |
denotes |
A7 |
T3588 |
10991-10996 |
VBN |
denotes |
found |
T3589 |
10987-10990 |
VBP |
denotes |
are |
T3590 |
10997-10999 |
IN |
denotes |
in |
T3591 |
11000-11005 |
JJ |
denotes |
adult |
T3592 |
11012-11018 |
NN |
denotes |
tissue |
T3593 |
11006-11011 |
NN |
denotes |
brain |
T3594 |
11019-11020 |
-LRB- |
denotes |
( |
T3595 |
11025-11027 |
NN |
denotes |
1B |
T3596 |
11020-11024 |
NN |
denotes |
Fig. |
T3597 |
11027-11028 |
-RRB- |
denotes |
) |
T3598 |
11028-11029 |
. |
denotes |
. |
T3599 |
11029-11322 |
sentence |
denotes |
In general, we observed two characteristic staining patterns for Annexin A7, a prominent nuclear stain in neurons (determined by lack of GFAP-immunoreactivity, localization, morphology) and a cytoplasmic and nuclear stain in astrocytes (GFAP-positive) in all areas of the mature murine brain. |
T3600 |
11030-11032 |
IN |
denotes |
In |
T3601 |
11045-11053 |
VBD |
denotes |
observed |
T3602 |
11033-11040 |
JJ |
denotes |
general |
T3603 |
11040-11042 |
, |
denotes |
, |
T3604 |
11042-11044 |
PRP |
denotes |
we |
T3605 |
11054-11057 |
CD |
denotes |
two |
T3606 |
11082-11090 |
NNS |
denotes |
patterns |
T3607 |
11058-11072 |
JJ |
denotes |
characteristic |
T3608 |
11073-11081 |
NN |
denotes |
staining |
T3609 |
11091-11094 |
IN |
denotes |
for |
T3610 |
11095-11102 |
NN |
denotes |
Annexin |
T3611 |
11103-11105 |
NN |
denotes |
A7 |
T3612 |
11105-11107 |
, |
denotes |
, |
T3613 |
11107-11108 |
DT |
denotes |
a |
T3614 |
11127-11132 |
NN |
denotes |
stain |
T3615 |
11109-11118 |
JJ |
denotes |
prominent |
T3616 |
11119-11126 |
JJ |
denotes |
nuclear |
T3617 |
11133-11135 |
IN |
denotes |
in |
T3618 |
11136-11143 |
NNS |
denotes |
neurons |
T3619 |
11144-11145 |
-LRB- |
denotes |
( |
T3620 |
11145-11155 |
VBN |
denotes |
determined |
T3621 |
11156-11158 |
IN |
denotes |
by |
T3622 |
11159-11163 |
NN |
denotes |
lack |
T3623 |
11164-11166 |
IN |
denotes |
of |
T3624 |
11167-11171 |
NN |
denotes |
GFAP |
T3625 |
11204-11214 |
NN |
denotes |
morphology |
T3626 |
11171-11172 |
HYPH |
denotes |
- |
T3627 |
11172-11188 |
NN |
denotes |
immunoreactivity |
T3628 |
11188-11190 |
, |
denotes |
, |
T3629 |
11190-11202 |
NN |
denotes |
localization |
T3630 |
11202-11204 |
, |
denotes |
, |
T3631 |
11214-11215 |
-RRB- |
denotes |
) |
T3632 |
11216-11219 |
CC |
denotes |
and |
T3633 |
11220-11221 |
DT |
denotes |
a |
T3634 |
11246-11251 |
NN |
denotes |
stain |
T3635 |
11222-11233 |
JJ |
denotes |
cytoplasmic |
T3636 |
11234-11237 |
CC |
denotes |
and |
T3637 |
11238-11245 |
JJ |
denotes |
nuclear |
T3638 |
11252-11254 |
IN |
denotes |
in |
T3639 |
11255-11265 |
NNS |
denotes |
astrocytes |
T3640 |
11266-11267 |
-LRB- |
denotes |
( |
T3641 |
11272-11280 |
JJ |
denotes |
positive |
T3642 |
11267-11271 |
NN |
denotes |
GFAP |
T3643 |
11271-11272 |
HYPH |
denotes |
- |
T3644 |
11280-11281 |
-RRB- |
denotes |
) |
T3645 |
11282-11284 |
IN |
denotes |
in |
T3646 |
11285-11288 |
DT |
denotes |
all |
T3647 |
11289-11294 |
NNS |
denotes |
areas |
T3648 |
11295-11297 |
IN |
denotes |
of |
T3649 |
11298-11301 |
DT |
denotes |
the |
T3650 |
11316-11321 |
NN |
denotes |
brain |
T3651 |
11302-11308 |
JJ |
denotes |
mature |
T3652 |
11309-11315 |
JJ |
denotes |
murine |
T3653 |
11321-11322 |
. |
denotes |
. |
T3654 |
11322-11484 |
sentence |
denotes |
In the neocortex (isocortex) Annexin A7 was strongly enriched in nuclei of neurons of all six cortical laminae (Fig. 4A, section derived from cortex temporalis). |
T3655 |
11323-11325 |
IN |
denotes |
In |
T3656 |
11376-11384 |
VBN |
denotes |
enriched |
T3657 |
11326-11329 |
DT |
denotes |
the |
T3658 |
11330-11339 |
NN |
denotes |
neocortex |
T3659 |
11340-11341 |
-LRB- |
denotes |
( |
T3660 |
11341-11350 |
NN |
denotes |
isocortex |
T3661 |
11350-11351 |
-RRB- |
denotes |
) |
T3662 |
11352-11359 |
NN |
denotes |
Annexin |
T3663 |
11360-11362 |
NN |
denotes |
A7 |
T3664 |
11363-11366 |
VBD |
denotes |
was |
T3665 |
11367-11375 |
RB |
denotes |
strongly |
T3666 |
11385-11387 |
IN |
denotes |
in |
T3667 |
11388-11394 |
NNS |
denotes |
nuclei |
T3668 |
11395-11397 |
IN |
denotes |
of |
T3669 |
11398-11405 |
NNS |
denotes |
neurons |
T3670 |
11406-11408 |
IN |
denotes |
of |
T3671 |
11409-11412 |
DT |
denotes |
all |
T3672 |
11426-11433 |
NNS |
denotes |
laminae |
T3673 |
11413-11416 |
CD |
denotes |
six |
T3674 |
11417-11425 |
JJ |
denotes |
cortical |
T3675 |
11434-11435 |
-LRB- |
denotes |
( |
T3676 |
11444-11451 |
NN |
denotes |
section |
T3677 |
11435-11439 |
NN |
denotes |
Fig. |
T3678 |
11440-11442 |
NN |
denotes |
4A |
T3679 |
11442-11444 |
, |
denotes |
, |
T3680 |
11452-11459 |
VBN |
denotes |
derived |
T3681 |
11460-11464 |
IN |
denotes |
from |
T3682 |
11465-11471 |
NN |
denotes |
cortex |
T3683 |
11472-11482 |
NN |
denotes |
temporalis |
T3684 |
11482-11483 |
-RRB- |
denotes |
) |
T3685 |
11483-11484 |
. |
denotes |
. |
T3686 |
11484-11591 |
sentence |
denotes |
For control, in the adult brain type-1 astrocytes can be characterized by a positive GFAP-stain (Fig. 4B). |
T3687 |
11485-11488 |
IN |
denotes |
For |
T3688 |
11542-11555 |
VBN |
denotes |
characterized |
T3689 |
11489-11496 |
NN |
denotes |
control |
T3690 |
11496-11498 |
, |
denotes |
, |
T3691 |
11498-11500 |
IN |
denotes |
in |
T3692 |
11501-11504 |
DT |
denotes |
the |
T3693 |
11511-11516 |
NN |
denotes |
brain |
T3694 |
11505-11510 |
JJ |
denotes |
adult |
T3695 |
11517-11521 |
NN |
denotes |
type |
T3696 |
11524-11534 |
NNS |
denotes |
astrocytes |
T3697 |
11521-11522 |
HYPH |
denotes |
- |
T3698 |
11522-11523 |
CD |
denotes |
1 |
T3699 |
11535-11538 |
MD |
denotes |
can |
T3700 |
11539-11541 |
VB |
denotes |
be |
T3701 |
11556-11558 |
IN |
denotes |
by |
T3702 |
11559-11560 |
DT |
denotes |
a |
T3703 |
11575-11580 |
NN |
denotes |
stain |
T3704 |
11561-11569 |
JJ |
denotes |
positive |
T3705 |
11570-11574 |
NN |
denotes |
GFAP |
T3706 |
11574-11575 |
HYPH |
denotes |
- |
T3707 |
11581-11582 |
-LRB- |
denotes |
( |
T3708 |
11587-11589 |
NN |
denotes |
4B |
T3709 |
11582-11586 |
NN |
denotes |
Fig. |
T3710 |
11589-11590 |
-RRB- |
denotes |
) |
T3711 |
11590-11591 |
. |
denotes |
. |
T3712 |
11591-11873 |
sentence |
denotes |
Fig. 4F demonstrates the presence of nuclear Annexin A7 in a neocortical neuron of the external granular layer (layer II) and in an astrocytic cell of the neocortical molecular layer (layer I) which additionally exhibits an intense signal in the cytoplasm and in cellular branches. |
T3713 |
11592-11596 |
NN |
denotes |
Fig. |
T3714 |
11597-11599 |
NN |
denotes |
4F |
T3715 |
11600-11612 |
VBZ |
denotes |
demonstrates |
T3716 |
11613-11616 |
DT |
denotes |
the |
T3717 |
11617-11625 |
NN |
denotes |
presence |
T3718 |
11626-11628 |
IN |
denotes |
of |
T3719 |
11629-11636 |
JJ |
denotes |
nuclear |
T3720 |
11645-11647 |
NN |
denotes |
A7 |
T3721 |
11637-11644 |
NN |
denotes |
Annexin |
T3722 |
11648-11650 |
IN |
denotes |
in |
T3723 |
11651-11652 |
DT |
denotes |
a |
T3724 |
11665-11671 |
NN |
denotes |
neuron |
T3725 |
11653-11664 |
JJ |
denotes |
neocortical |
T3726 |
11672-11674 |
IN |
denotes |
of |
T3727 |
11675-11678 |
DT |
denotes |
the |
T3728 |
11697-11702 |
NN |
denotes |
layer |
T3729 |
11679-11687 |
JJ |
denotes |
external |
T3730 |
11688-11696 |
JJ |
denotes |
granular |
T3731 |
11703-11704 |
-LRB- |
denotes |
( |
T3732 |
11704-11709 |
NN |
denotes |
layer |
T3733 |
11710-11712 |
CD |
denotes |
II |
T3734 |
11712-11713 |
-RRB- |
denotes |
) |
T3735 |
11714-11717 |
CC |
denotes |
and |
T3736 |
11718-11720 |
IN |
denotes |
in |
T3737 |
11721-11723 |
DT |
denotes |
an |
T3738 |
11735-11739 |
NN |
denotes |
cell |
T3739 |
11724-11734 |
JJ |
denotes |
astrocytic |
T3740 |
11740-11742 |
IN |
denotes |
of |
T3741 |
11743-11746 |
DT |
denotes |
the |
T3742 |
11769-11774 |
NN |
denotes |
layer |
T3775 |
11944-11946 |
IN |
denotes |
in |
T3776 |
11947-11951 |
CC |
denotes |
both |
T3777 |
11953-11960 |
NN |
denotes |
nucleus |
T3778 |
11951-11953 |
, |
denotes |
, |
T3779 |
11961-11964 |
CC |
denotes |
and |
T3780 |
11965-11972 |
NN |
denotes |
cytosol |
T3781 |
11972-11974 |
, |
denotes |
, |
T3782 |
11974-11977 |
CC |
denotes |
and |
T3783 |
11978-11981 |
VBD |
denotes |
was |
T3784 |
11982-11992 |
VBN |
denotes |
identified |
T3785 |
11993-11995 |
IN |
denotes |
as |
T3786 |
11996-12005 |
JJ |
denotes |
pyramidal |
T3787 |
12006-12012 |
NN |
denotes |
neuron |
T3788 |
12013-12018 |
VBN |
denotes |
based |
T3789 |
12019-12021 |
IN |
denotes |
on |
T3790 |
12022-12032 |
NN |
denotes |
morphology |
T3791 |
12033-12036 |
CC |
denotes |
and |
T3792 |
12037-12044 |
NN |
denotes |
absence |
T3793 |
12045-12047 |
IN |
denotes |
of |
T3794 |
12048-12052 |
NN |
denotes |
GFAP |
T3795 |
12053-12069 |
NN |
denotes |
immunoreactivity |
T3796 |
12069-12070 |
. |
denotes |
. |
T3797 |
12070-12144 |
sentence |
denotes |
However, Annexin A7 apparently is more enriched in the nucleus (Fig. 4E). |
T3798 |
12071-12078 |
RB |
denotes |
However |
T3799 |
12102-12104 |
VBZ |
denotes |
is |
T3800 |
12078-12080 |
, |
denotes |
, |
T3801 |
12080-12087 |
NN |
denotes |
Annexin |
T3802 |
12088-12090 |
NN |
denotes |
A7 |
T3803 |
12091-12101 |
RB |
denotes |
apparently |
T3804 |
12105-12109 |
RBR |
denotes |
more |
T3805 |
12110-12118 |
JJ |
denotes |
enriched |
T3806 |
12119-12121 |
IN |
denotes |
in |
T3807 |
12122-12125 |
DT |
denotes |
the |
T3808 |
12126-12133 |
NN |
denotes |
nucleus |
T3809 |
12134-12135 |
-LRB- |
denotes |
( |
T3810 |
12140-12142 |
NN |
denotes |
4E |
T3811 |
12135-12139 |
NN |
denotes |
Fig. |
T3812 |
12142-12143 |
-RRB- |
denotes |
) |
T3813 |
12143-12144 |
. |
denotes |
. |
T3814 |
12144-12145 |
sentence |
denotes |
|
T3843 |
14115-14119 |
NN |
denotes |
Fig. |
T3844 |
14122-14123 |
-RRB- |
denotes |
) |
T3845 |
14123-14124 |
. |
denotes |
. |
T3846 |
14124-14302 |
sentence |
denotes |
The astrocytes are mainly localized in the area between cells exhibiting nuclear Annexin A7 staining and show the protein also in the nucleus and the cytoplasm (data not shown). |
T3847 |
14125-14128 |
DT |
denotes |
The |
T3848 |
14129-14139 |
NNS |
denotes |
astrocytes |
T3849 |
14151-14160 |
VBN |
denotes |
localized |
T3850 |
14140-14143 |
VBP |
denotes |
are |
T3851 |
14144-14150 |
RB |
denotes |
mainly |
T3852 |
14161-14163 |
IN |
denotes |
in |
T3853 |
14164-14167 |
DT |
denotes |
the |
T3854 |
14168-14172 |
NN |
denotes |
area |
T3855 |
14173-14180 |
IN |
denotes |
between |
T3856 |
14181-14186 |
NNS |
denotes |
cells |
T3857 |
14187-14197 |
VBG |
denotes |
exhibiting |
T3858 |
14198-14205 |
JJ |
denotes |
nuclear |
T3859 |
14217-14225 |
NN |
denotes |
staining |
T3860 |
14206-14213 |
NN |
denotes |
Annexin |
T3861 |
14214-14216 |
NN |
denotes |
A7 |
T3862 |
14226-14229 |
CC |
denotes |
and |
T3863 |
14230-14234 |
VBP |
denotes |
show |
T3864 |
14235-14238 |
DT |
denotes |
the |
T3865 |
14239-14246 |
NN |
denotes |
protein |
T3866 |
14247-14251 |
RB |
denotes |
also |
T3867 |
14252-14254 |
IN |
denotes |
in |
T3868 |
14255-14258 |
DT |
denotes |
the |
T3869 |
14259-14266 |
NN |
denotes |
nucleus |
T3870 |
14267-14270 |
CC |
denotes |
and |
T3871 |
14271-14274 |
DT |
denotes |
the |
T3872 |
14275-14284 |
NN |
denotes |
cytoplasm |
T3873 |
14285-14286 |
-LRB- |
denotes |
( |
T3874 |
14295-14300 |
VBN |
denotes |
shown |
T3875 |
14286-14290 |
NNS |
denotes |
data |
T3876 |
14291-14294 |
RB |
denotes |
not |
T3877 |
14300-14301 |
-RRB- |
denotes |
) |
T3878 |
14301-14302 |
. |
denotes |
. |
T3879 |
14302-14459 |
sentence |
denotes |
When we analyzed the pyramidal neurons in the hippocampus at a higher magnification we found that Annexin A7 again mainly localized to the nuclei (Fig. 4G). |
T3880 |
14303-14307 |
WRB |
denotes |
When |
T3881 |
14311-14319 |
VBD |
denotes |
analyzed |
T3882 |
14308-14310 |
PRP |
denotes |
we |
T3883 |
14390-14395 |
VBD |
denotes |
found |
T3884 |
14320-14323 |
DT |
denotes |
the |
T3885 |
14334-14341 |
NNS |
denotes |
neurons |
T3886 |
14324-14333 |
JJ |
denotes |
pyramidal |
T3887 |
14342-14344 |
IN |
denotes |
in |
T3888 |
14345-14348 |
DT |
denotes |
the |
T3889 |
14349-14360 |
NN |
denotes |
hippocampus |
T3890 |
14361-14363 |
IN |
denotes |
at |
T3891 |
14364-14365 |
DT |
denotes |
a |
T3892 |
14373-14386 |
NN |
denotes |
magnification |
T3893 |
14366-14372 |
JJR |
denotes |
higher |
T3894 |
14387-14389 |
PRP |
denotes |
we |
T3895 |
14396-14400 |
IN |
denotes |
that |
T3896 |
14425-14434 |
VBN |
denotes |
localized |
T3897 |
14401-14408 |
NN |
denotes |
Annexin |
T3898 |
14409-14411 |
NN |
denotes |
A7 |
T3899 |
14412-14417 |
RB |
denotes |
again |
T3900 |
14418-14424 |
RB |
denotes |
mainly |
T3901 |
14435-14437 |
IN |
denotes |
to |
T3902 |
14438-14441 |
DT |
denotes |
the |
T3903 |
14442-14448 |
NNS |
denotes |
nuclei |
T3904 |
14449-14450 |
-LRB- |
denotes |
( |
T3905 |
14455-14457 |
NN |
denotes |
4G |
T3906 |
14450-14454 |
NN |
denotes |
Fig. |
T3907 |
14457-14458 |
-RRB- |
denotes |
) |
T3908 |
14458-14459 |
. |
denotes |
. |
T3909 |
14459-14531 |
sentence |
denotes |
Polyclonal antibodies gave a similar staining pattern (data not shown). |
T3910 |
14460-14470 |
JJ |
denotes |
Polyclonal |
T3911 |
14471-14481 |
NNS |
denotes |
antibodies |
T3912 |
14482-14486 |
VBD |
denotes |
gave |
T3913 |
14487-14488 |
DT |
denotes |
a |
T3914 |
14506-14513 |
NN |
denotes |
pattern |
T3915 |
14489-14496 |
JJ |
denotes |
similar |
T3916 |
14497-14505 |
NN |
denotes |
staining |
T3917 |
14514-14515 |
-LRB- |
denotes |
( |
T3918 |
14524-14529 |
VBN |
denotes |
shown |
T3919 |
14515-14519 |
NNS |
denotes |
data |
T3920 |
14520-14523 |
RB |
denotes |
not |
T3921 |
14529-14530 |
-RRB- |
denotes |
) |
T3922 |
14530-14531 |
. |
denotes |
. |
T3923 |
14531-14723 |
sentence |
denotes |
The intense nuclear staining was absent in controls either stained only with the secondary antibody (Fig. 4D) or in brain sections derived from AnxA7-/- mice stained for Annexin A7 (Fig. 4H). |
T3924 |
14532-14535 |
DT |
denotes |
The |
T3925 |
14552-14560 |
NN |
denotes |
staining |
T3926 |
14536-14543 |
JJ |
denotes |
intense |
T3927 |
14544-14551 |
JJ |
denotes |
nuclear |
T3928 |
14561-14564 |
VBD |
denotes |
was |
T3929 |
14565-14571 |
JJ |
denotes |
absent |
T3930 |
14572-14574 |
IN |
denotes |
in |
T3931 |
14575-14583 |
NNS |
denotes |
controls |
T3932 |
14584-14590 |
CC |
denotes |
either |
T3933 |
14591-14598 |
VBN |
denotes |
stained |
T3934 |
14599-14603 |
RB |
denotes |
only |
T3935 |
14604-14608 |
IN |
denotes |
with |
T3936 |
14609-14612 |
DT |
denotes |
the |
T3937 |
14623-14631 |
NN |
denotes |
antibody |
T3938 |
14613-14622 |
JJ |
denotes |
secondary |
T3939 |
14632-14633 |
-LRB- |
denotes |
( |
T3940 |
14638-14640 |
NN |
denotes |
4D |
T3941 |
14633-14637 |
NN |
denotes |
Fig. |
T3942 |
14640-14641 |
-RRB- |
denotes |
) |
T3943 |
14642-14644 |
CC |
denotes |
or |
T3944 |
14645-14647 |
IN |
denotes |
in |
T3945 |
14648-14653 |
NN |
denotes |
brain |
T3946 |
14654-14662 |
NNS |
denotes |
sections |
T3947 |
14663-14670 |
VBN |
denotes |
derived |
T3948 |
14671-14675 |
IN |
denotes |
from |
T3949 |
14676-14681 |
NN |
denotes |
AnxA7 |
T3950 |
14685-14689 |
NNS |
denotes |
mice |
T3951 |
14681-14682 |
SYM |
denotes |
- |
T3952 |
14682-14683 |
HYPH |
denotes |
/ |
T3953 |
14683-14684 |
SYM |
denotes |
- |
T3954 |
14690-14697 |
VBN |
denotes |
stained |
T3955 |
14698-14701 |
IN |
denotes |
for |
T3956 |
14702-14709 |
NN |
denotes |
Annexin |
T3957 |
14710-14712 |
NN |
denotes |
A7 |
T3958 |
14713-14714 |
-LRB- |
denotes |
( |
T3959 |
14719-14721 |
NN |
denotes |
4H |
T3960 |
14714-14718 |
NN |
denotes |
Fig. |
T3961 |
14721-14722 |
-RRB- |
denotes |
) |
T3962 |
14722-14723 |
. |
denotes |
. |
T3963 |
14723-14873 |
sentence |
denotes |
The residual staining seen in the AnxA7-/- brain is unspecific as it is also present in corresponding negative controls lacking the primary antibody. |
T3964 |
14724-14727 |
DT |
denotes |
The |
T3965 |
14737-14745 |
NN |
denotes |
staining |
T3966 |
14728-14736 |
JJ |
denotes |
residual |
T3967 |
14773-14775 |
VBZ |
denotes |
is |
T3968 |
14746-14750 |
VBN |
denotes |
seen |
T3969 |
14751-14753 |
IN |
denotes |
in |
T3970 |
14754-14757 |
DT |
denotes |
the |
T3971 |
14767-14772 |
NN |
denotes |
brain |
T3972 |
14758-14763 |
NN |
denotes |
AnxA7 |
T3973 |
14763-14764 |
SYM |
denotes |
- |
T3974 |
14764-14765 |
HYPH |
denotes |
/ |
T3975 |
14765-14766 |
SYM |
denotes |
- |
T3976 |
14776-14786 |
JJ |
denotes |
unspecific |
T3977 |
14787-14789 |
IN |
denotes |
as |
T3978 |
14793-14795 |
VBZ |
denotes |
is |
T3979 |
14790-14792 |
PRP |
denotes |
it |
T3980 |
14796-14800 |
RB |
denotes |
also |
T3981 |
14801-14808 |
JJ |
denotes |
present |
T3982 |
14809-14811 |
IN |
denotes |
in |
T3983 |
14812-14825 |
VBG |
denotes |
corresponding |
T3984 |
14835-14843 |
NNS |
denotes |
controls |
T3985 |
14826-14834 |
JJ |
denotes |
negative |
T3986 |
14844-14851 |
VBG |
denotes |
lacking |
T3987 |
14852-14855 |
DT |
denotes |
the |
T3988 |
14864-14872 |
NN |
denotes |
antibody |
T3989 |
14856-14863 |
JJ |
denotes |
primary |
T3990 |
14872-14873 |
. |
denotes |
. |
T3991 |
14873-14983 |
sentence |
denotes |
The absence of Annexin A7 protein in brain and other tissues of the AnxA7-/- mouse has been verified in [10]. |
T3992 |
14874-14877 |
DT |
denotes |
The |
T3993 |
14878-14885 |
NN |
denotes |
absence |
T3994 |
14966-14974 |
VBN |
denotes |
verified |
T3995 |
14886-14888 |
IN |
denotes |
of |
T3996 |
14889-14896 |
NN |
denotes |
Annexin |
T3997 |
14897-14899 |
NN |
denotes |
A7 |
T3998 |
14900-14907 |
NN |
denotes |
protein |
T3999 |
14908-14910 |
IN |
denotes |
in |
T4000 |
14911-14916 |
NN |
denotes |
brain |
T4001 |
14917-14920 |
CC |
denotes |
and |
T4002 |
14921-14926 |
JJ |
denotes |
other |
T4003 |
14927-14934 |
NNS |
denotes |
tissues |
T4004 |
14935-14937 |
IN |
denotes |
of |
T4005 |
14938-14941 |
DT |
denotes |
the |
T4006 |
14951-14956 |
NN |
denotes |
mouse |
T4007 |
14942-14947 |
NN |
denotes |
AnxA7 |
T4008 |
14947-14948 |
SYM |
denotes |
- |
T4009 |
14948-14949 |
HYPH |
denotes |
/ |
T4010 |
14949-14950 |
SYM |
denotes |
- |
T4011 |
14957-14960 |
VBZ |
denotes |
has |
T4012 |
14961-14965 |
VBN |
denotes |
been |
T4013 |
14975-14977 |
IN |
denotes |
in |
T4014 |
14978-14979 |
-LRB- |
denotes |
[ |
T4015 |
14979-14981 |
CD |
denotes |
10 |
T4016 |
14981-14982 |
-RRB- |
denotes |
] |
T4017 |
14982-14983 |
. |
denotes |
. |
T4018 |
14983-15104 |
sentence |
denotes |
In another actual study of the knock out mouse we detected a thickened basal membrane and a widened intercellular space. |
T4019 |
14984-14986 |
IN |
denotes |
In |
T4020 |
15034-15042 |
VBD |
denotes |
detected |
T4021 |
14987-14994 |
DT |
denotes |
another |
T4022 |
15002-15007 |
NN |
denotes |
study |
T4023 |
14995-15001 |
JJ |
denotes |
actual |
T4024 |
15008-15010 |
IN |
denotes |
of |
T4025 |
15011-15014 |
DT |
denotes |
the |
T4026 |
15025-15030 |
NN |
denotes |
mouse |
T4027 |
15015-15020 |
VB |
denotes |
knock |
T4028 |
15021-15024 |
RP |
denotes |
out |
T4029 |
15031-15033 |
PRP |
denotes |
we |
T4030 |
15043-15044 |
DT |
denotes |
a |
T4031 |
15061-15069 |
NN |
denotes |
membrane |
T4032 |
15045-15054 |
VBN |
denotes |
thickened |
T4033 |
15055-15060 |
JJ |
denotes |
basal |
T4034 |
15070-15073 |
CC |
denotes |
and |
T4035 |
15074-15075 |
DT |
denotes |
a |
T4036 |
15098-15103 |
NN |
denotes |
space |
T4037 |
15076-15083 |
VBN |
denotes |
widened |
T4038 |
15084-15097 |
JJ |
denotes |
intercellular |
T4039 |
15103-15104 |
. |
denotes |
. |
T4040 |
15104-15165 |
sentence |
denotes |
This may cause unspecific binding of the secondary antibody. |
T4041 |
15105-15109 |
DT |
denotes |
This |
T4042 |
15114-15119 |
VB |
denotes |
cause |
T4043 |
15110-15113 |
MD |
denotes |
may |
T4044 |
15120-15130 |
JJ |
denotes |
unspecific |
T4045 |
15131-15138 |
NN |
denotes |
binding |
T4046 |
15139-15141 |
IN |
denotes |
of |
T4047 |
15142-15145 |
DT |
denotes |
the |
T4048 |
15156-15164 |
NN |
denotes |
antibody |
T4049 |
15146-15155 |
JJ |
denotes |
secondary |
T4050 |
15164-15165 |
. |
denotes |
. |
T4051 |
15165-15241 |
sentence |
denotes |
An equal staining of Annexin A7 was also found in the cerebellum (Fig. 5A). |
T4052 |
15166-15168 |
DT |
denotes |
An |
T4053 |
15175-15183 |
NN |
denotes |
staining |
T4054 |
15169-15174 |
JJ |
denotes |
equal |
T4055 |
15207-15212 |
VBN |
denotes |
found |
T4056 |
15184-15186 |
IN |
denotes |
of |
T4057 |
15187-15194 |
NN |
denotes |
Annexin |
T4058 |
15195-15197 |
NN |
denotes |
A7 |
T4059 |
15198-15201 |
VBD |
denotes |
was |
T4060 |
15202-15206 |
RB |
denotes |
also |
T4061 |
15213-15215 |
IN |
denotes |
in |
T4062 |
15216-15219 |
DT |
denotes |
the |
T4063 |
15220-15230 |
NN |
denotes |
cerebellum |
T4064 |
15231-15232 |
-LRB- |
denotes |
( |
T4065 |
15237-15239 |
NN |
denotes |
5A |
T4066 |
15232-15236 |
NN |
denotes |
Fig. |
T4067 |
15239-15240 |
-RRB- |
denotes |
) |
T4068 |
15240-15241 |
. |
denotes |
. |
T4069 |
15241-15331 |
sentence |
denotes |
Nuclei of neurons in the stratum granulosum show the most prominent staining (Fig. 5C,D). |
T4070 |
15242-15248 |
NNS |
denotes |
Nuclei |
T4071 |
15286-15290 |
VBP |
denotes |
show |
T4072 |
15249-15251 |
IN |
denotes |
of |
T4073 |
15252-15259 |
NNS |
denotes |
neurons |
T4074 |
15260-15262 |
IN |
denotes |
in |
T4075 |
15263-15266 |
DT |
denotes |
the |
T4076 |
15275-15285 |
NN |
denotes |
granulosum |
T4077 |
15267-15274 |
NN |
denotes |
stratum |
T4078 |
15291-15294 |
DT |
denotes |
the |
T4079 |
15310-15318 |
NN |
denotes |
staining |
T4080 |
15295-15299 |
RBS |
denotes |
most |
T4081 |
15300-15309 |
JJ |
denotes |
prominent |
T4082 |
15319-15320 |
-LRB- |
denotes |
( |
T4083 |
15328-15329 |
NN |
denotes |
D |
T4084 |
15320-15324 |
NN |
denotes |
Fig. |
T4085 |
15325-15327 |
NN |
denotes |
5C |
T4086 |
15327-15328 |
, |
denotes |
, |
T4087 |
15329-15330 |
-RRB- |
denotes |
) |
T4088 |
15330-15331 |
. |
denotes |
. |
T4089 |
15331-15466 |
sentence |
denotes |
In the stratum moleculare, which is poor in cell bodies of neurons, only a few dots appear which also correspond to nuclei of neurons. |
T4090 |
15332-15334 |
IN |
denotes |
In |
T4091 |
15416-15422 |
VBP |
denotes |
appear |
T4092 |
15335-15338 |
DT |
denotes |
the |
T4093 |
15347-15357 |
NN |
denotes |
moleculare |
T4094 |
15339-15346 |
NN |
denotes |
stratum |
T4095 |
15357-15359 |
, |
denotes |
, |
T4096 |
15359-15364 |
WDT |
denotes |
which |
T4097 |
15365-15367 |
VBZ |
denotes |
is |
T4098 |
15368-15372 |
JJ |
denotes |
poor |
T4099 |
15373-15375 |
IN |
denotes |
in |
T4100 |
15376-15380 |
NN |
denotes |
cell |
T4101 |
15381-15387 |
NNS |
denotes |
bodies |
T4102 |
15388-15390 |
IN |
denotes |
of |
T4103 |
15391-15398 |
NNS |
denotes |
neurons |
T4104 |
15398-15400 |
, |
denotes |
, |
T4105 |
15400-15404 |
RB |
denotes |
only |
T4106 |
15411-15415 |
NNS |
denotes |
dots |
T4107 |
15405-15406 |
DT |
denotes |
a |
T4108 |
15407-15410 |
JJ |
denotes |
few |
T4109 |
15423-15428 |
WDT |
denotes |
which |
T4110 |
15434-15444 |
VBP |
denotes |
correspond |
T4111 |
15429-15433 |
RB |
denotes |
also |
T4112 |
15445-15447 |
IN |
denotes |
to |
T4113 |
15448-15454 |
NNS |
denotes |
nuclei |
T4114 |
15455-15457 |
IN |
denotes |
of |
T4115 |
15458-15465 |
NNS |
denotes |
neurons |
T4116 |
15465-15466 |
. |
denotes |
. |
T4117 |
15466-15556 |
sentence |
denotes |
Astrocytes are also positive for Annexin A7 in the nuclei and cytoplasm (data not shown). |
T4118 |
15467-15477 |
NNS |
denotes |
Astrocytes |
T4119 |
15478-15481 |
VBP |
denotes |
are |
T4120 |
15482-15486 |
RB |
denotes |
also |
T4121 |
15487-15495 |
JJ |
denotes |
positive |
T4122 |
15496-15499 |
IN |
denotes |
for |
T4123 |
15500-15507 |
NN |
denotes |
Annexin |
T4124 |
15508-15510 |
NN |
denotes |
A7 |
T4125 |
15511-15513 |
IN |
denotes |
in |
T4126 |
15514-15517 |
DT |
denotes |
the |
T4127 |
15518-15524 |
NNS |
denotes |
nuclei |
T4128 |
15525-15528 |
CC |
denotes |
and |
T4129 |
15529-15538 |
NN |
denotes |
cytoplasm |
T4130 |
15539-15540 |
-LRB- |
denotes |
( |
T4131 |
15549-15554 |
VBN |
denotes |
shown |
T4132 |
15540-15544 |
NNS |
denotes |
data |
T4133 |
15545-15548 |
RB |
denotes |
not |
T4134 |
15554-15555 |
-RRB- |
denotes |
) |
T4135 |
15555-15556 |
. |
denotes |
. |
T4136 |
15556-15714 |
sentence |
denotes |
The signal of the pial border and the prominent stain of the white matter tracts (laminae medullares) are due to Annexin A7 positive astrocytes (Fig. 5 A–C). |
T4137 |
15557-15560 |
DT |
denotes |
The |
T4138 |
15561-15567 |
NN |
denotes |
signal |
T4139 |
15659-15662 |
VBP |
denotes |
are |
T4140 |
15568-15570 |
IN |
denotes |
of |
T4141 |
15571-15574 |
DT |
denotes |
the |
T4142 |
15580-15586 |
NN |
denotes |
border |
T4143 |
15575-15579 |
JJ |
denotes |
pial |
T4144 |
15587-15590 |
CC |
denotes |
and |
T4145 |
15591-15594 |
DT |
denotes |
the |
T4146 |
15605-15610 |
NN |
denotes |
stain |
T4147 |
15595-15604 |
JJ |
denotes |
prominent |
T4148 |
15611-15613 |
IN |
denotes |
of |
T4149 |
15614-15617 |
DT |
denotes |
the |
T4150 |
15631-15637 |
NNS |
denotes |
tracts |
T4151 |
15618-15623 |
JJ |
denotes |
white |
T4152 |
15624-15630 |
NN |
denotes |
matter |
T4153 |
15638-15639 |
-LRB- |
denotes |
( |
T4154 |
15639-15646 |
NNS |
denotes |
laminae |
T4155 |
15647-15657 |
NNS |
denotes |
medullares |
T4156 |
15657-15658 |
-RRB- |
denotes |
) |
T4157 |
15663-15666 |
IN |
denotes |
due |
T4158 |
15667-15669 |
IN |
denotes |
to |
T4159 |
15670-15677 |
NN |
denotes |
Annexin |
T4160 |
15678-15680 |
NN |
denotes |
A7 |
T4161 |
15681-15689 |
JJ |
denotes |
positive |
T4162 |
15690-15700 |
NNS |
denotes |
astrocytes |
T4163 |
15701-15702 |
-LRB- |
denotes |
( |
T4164 |
15709-15710 |
NN |
denotes |
A |
T4165 |
15702-15706 |
NN |
denotes |
Fig. |
T4166 |
15707-15708 |
CD |
denotes |
5 |
T4167 |
15710-15711 |
SYM |
denotes |
– |
T4168 |
15711-15712 |
NN |
denotes |
C |
T4169 |
15712-15713 |
-RRB- |
denotes |
) |
T4170 |
15713-15714 |
. |
denotes |
. |
T4171 |
15714-15932 |
sentence |
denotes |
Higher magnification of the boundary layer between stratum moleculare and granulosum (Fig. 5D) revealed an Annexin A7 signal in nuclei, but also in the cytoplasm and at the plasma membrane of Purkinje cells (Fig. 5E). |
T4172 |
15715-15721 |
JJR |
denotes |
Higher |
T4173 |
15722-15735 |
NN |
denotes |
magnification |
T4174 |
15810-15818 |
VBD |
denotes |
revealed |
T4175 |
15736-15738 |
IN |
denotes |
of |
T4176 |
15739-15742 |
DT |
denotes |
the |
T4177 |
15752-15757 |
NN |
denotes |
layer |
T4178 |
15743-15751 |
JJ |
denotes |
boundary |
T4179 |
15758-15765 |
IN |
denotes |
between |
T4180 |
15766-15773 |
NN |
denotes |
stratum |
T4181 |
15774-15784 |
NN |
denotes |
moleculare |
T4182 |
15785-15788 |
CC |
denotes |
and |
T4183 |
15789-15799 |
NN |
denotes |
granulosum |
T4184 |
15800-15801 |
-LRB- |
denotes |
( |
T4185 |
15806-15808 |
NN |
denotes |
5D |
T4186 |
15801-15805 |
NN |
denotes |
Fig. |
T4187 |
15808-15809 |
-RRB- |
denotes |
) |
T4188 |
15819-15821 |
DT |
denotes |
an |
T4189 |
15833-15839 |
NN |
denotes |
signal |
T4190 |
15822-15829 |
NN |
denotes |
Annexin |
T4191 |
15830-15832 |
NN |
denotes |
A7 |
T4192 |
15840-15842 |
IN |
denotes |
in |
T4193 |
15843-15849 |
NNS |
denotes |
nuclei |
T4194 |
15849-15851 |
, |
denotes |
, |
T4195 |
15851-15854 |
CC |
denotes |
but |
T4196 |
15855-15859 |
RB |
denotes |
also |
T4197 |
15860-15862 |
IN |
denotes |
in |
T4198 |
15863-15866 |
DT |
denotes |
the |
T4199 |
15867-15876 |
NN |
denotes |
cytoplasm |
T4200 |
15877-15880 |
CC |
denotes |
and |
T4201 |
15881-15883 |
IN |
denotes |
at |
T4202 |
15884-15887 |
DT |
denotes |
the |
T4203 |
15895-15903 |
NN |
denotes |
membrane |
T4204 |
15888-15894 |
NN |
denotes |
plasma |
T4205 |
15904-15906 |
IN |
denotes |
of |
T4206 |
15907-15915 |
NNP |
denotes |
Purkinje |
T4207 |
15916-15921 |
NNS |
denotes |
cells |
T4208 |
15922-15923 |
-LRB- |
denotes |
( |
T4209 |
15928-15930 |
NN |
denotes |
5E |
T4210 |
15923-15927 |
NN |
denotes |
Fig. |
T4211 |
15930-15931 |
-RRB- |
denotes |
) |
T4212 |
15931-15932 |
. |
denotes |
. |
T4213 |
15932-16009 |
sentence |
denotes |
The typical pair of dendrites points to the margin of the cerebellar cortex. |
T4214 |
15933-15936 |
DT |
denotes |
The |
T4215 |
15945-15949 |
NN |
denotes |
pair |
T4216 |
15937-15944 |
JJ |
denotes |
typical |
T4217 |
15963-15969 |
VBZ |
denotes |
points |
T4218 |
15950-15952 |
IN |
denotes |
of |
T4219 |
15953-15962 |
NNS |
denotes |
dendrites |
T4220 |
15970-15972 |
IN |
denotes |
to |
T4221 |
15973-15976 |
DT |
denotes |
the |
T4222 |
15977-15983 |
NN |
denotes |
margin |
T4223 |
15984-15986 |
IN |
denotes |
of |
T4224 |
15987-15990 |
DT |
denotes |
the |
T4225 |
16002-16008 |
NN |
denotes |
cortex |
T4226 |
15991-16001 |
JJ |
denotes |
cerebellar |
T4227 |
16008-16009 |
. |
denotes |
. |
T4228 |
16009-16131 |
sentence |
denotes |
The specificity of the Annexin A7 signal was further confirmed in similar brain sections of the AnxA7-/- mouse (Fig. 5F). |
T4229 |
16010-16013 |
DT |
denotes |
The |
T4230 |
16014-16025 |
NN |
denotes |
specificity |
T4231 |
16063-16072 |
VBN |
denotes |
confirmed |
T4232 |
16026-16028 |
IN |
denotes |
of |
T4233 |
16029-16032 |
DT |
denotes |
the |
T4234 |
16044-16050 |
NN |
denotes |
signal |
T4235 |
16033-16040 |
NN |
denotes |
Annexin |
T4236 |
16041-16043 |
NN |
denotes |
A7 |
T4237 |
16051-16054 |
VBD |
denotes |
was |
T4238 |
16055-16062 |
RBR |
denotes |
further |
T4239 |
16073-16075 |
IN |
denotes |
in |
T4240 |
16076-16083 |
JJ |
denotes |
similar |
T4241 |
16090-16098 |
NNS |
denotes |
sections |
T4242 |
16084-16089 |
NN |
denotes |
brain |
T4243 |
16099-16101 |
IN |
denotes |
of |
T4244 |
16102-16105 |
DT |
denotes |
the |
T4245 |
16115-16120 |
NN |
denotes |
mouse |
T4246 |
16106-16111 |
NN |
denotes |
AnxA7 |
T4247 |
16111-16112 |
SYM |
denotes |
- |
T4248 |
16112-16113 |
HYPH |
denotes |
/ |
T4249 |
16113-16114 |
SYM |
denotes |
- |
T4250 |
16121-16122 |
-LRB- |
denotes |
( |
T4251 |
16127-16129 |
NN |
denotes |
5F |
T4252 |
16122-16126 |
NN |
denotes |
Fig. |
T4253 |
16129-16130 |
-RRB- |
denotes |
) |
T4254 |
16130-16131 |
. |
denotes |
. |
T4255 |
16131-16240 |
sentence |
denotes |
Fig. 5G shows Annexin A7 in neurites (axons) connecting the laminae medullares with the Purkinje-cell layer. |
T4256 |
16132-16136 |
NN |
denotes |
Fig. |
T4257 |
16137-16139 |
NN |
denotes |
5G |
T4258 |
16140-16145 |
VBZ |
denotes |
shows |
T4259 |
16146-16153 |
NN |
denotes |
Annexin |
T4260 |
16154-16156 |
NN |
denotes |
A7 |
T4261 |
16157-16159 |
IN |
denotes |
in |
T4262 |
16160-16168 |
NNS |
denotes |
neurites |
T4263 |
16169-16170 |
-LRB- |
denotes |
( |
T4264 |
16170-16175 |
NNS |
denotes |
axons |
T4265 |
16175-16176 |
-RRB- |
denotes |
) |
T4266 |
16177-16187 |
VBG |
denotes |
connecting |
T4267 |
16188-16191 |
DT |
denotes |
the |
T4268 |
16200-16210 |
NNS |
denotes |
medullares |
T4269 |
16192-16199 |
NNS |
denotes |
laminae |
T4270 |
16211-16215 |
IN |
denotes |
with |
T4271 |
16216-16219 |
DT |
denotes |
the |
T4272 |
16234-16239 |
NN |
denotes |
layer |
T4273 |
16220-16228 |
NNP |
denotes |
Purkinje |
T4274 |
16229-16233 |
NN |
denotes |
cell |
T4275 |
16228-16229 |
HYPH |
denotes |
- |
T4276 |
16239-16240 |
. |
denotes |
. |
T4277 |
16240-16295 |
sentence |
denotes |
Most likely these are the axons of the Purkinje-cells. |
T4278 |
16241-16245 |
RBS |
denotes |
Most |
T4279 |
16246-16252 |
RB |
denotes |
likely |
T4280 |
16259-16262 |
VBP |
denotes |
are |
T4281 |
16253-16258 |
DT |
denotes |
these |
T4282 |
16263-16266 |
DT |
denotes |
the |
T4283 |
16267-16272 |
NNS |
denotes |
axons |
T4284 |
16273-16275 |
IN |
denotes |
of |
T4285 |
16276-16279 |
DT |
denotes |
the |
T4286 |
16289-16294 |
NNS |
denotes |
cells |
T4287 |
16280-16288 |
NNP |
denotes |
Purkinje |
T4288 |
16288-16289 |
HYPH |
denotes |
- |
T4289 |
16294-16295 |
. |
denotes |
. |
T4290 |
16295-16425 |
sentence |
denotes |
Apart from these efferent neurites the laminae medullares (lamina alba, white matter) contain afferent mossy and climbing fibers. |
T4291 |
16296-16301 |
RB |
denotes |
Apart |
T4292 |
16382-16389 |
VBP |
denotes |
contain |
T4293 |
16302-16306 |
IN |
denotes |
from |
T4294 |
16307-16312 |
DT |
denotes |
these |
T4295 |
16322-16330 |
NNS |
denotes |
neurites |
T4296 |
16313-16321 |
JJ |
denotes |
efferent |
T4297 |
16331-16334 |
DT |
denotes |
the |
T4298 |
16343-16353 |
NNS |
denotes |
medullares |
T4299 |
16335-16342 |
NNS |
denotes |
laminae |
T4300 |
16354-16355 |
-LRB- |
denotes |
( |
T4301 |
16374-16380 |
NN |
denotes |
matter |
T4302 |
16355-16361 |
NN |
denotes |
lamina |
T4303 |
16362-16366 |
NN |
denotes |
alba |
T4304 |
16366-16368 |
, |
denotes |
, |
T4305 |
16368-16373 |
JJ |
denotes |
white |
T4306 |
16380-16381 |
-RRB- |
denotes |
) |
T4307 |
16390-16398 |
JJ |
denotes |
afferent |
T4308 |
16418-16424 |
NNS |
denotes |
fibers |
T4309 |
16399-16404 |
JJ |
denotes |
mossy |
T4310 |
16405-16408 |
CC |
denotes |
and |
T4311 |
16409-16417 |
VBG |
denotes |
climbing |
T4312 |
16424-16425 |
. |
denotes |
. |
T4313 |
16425-16539 |
sentence |
denotes |
Thus, the intense stain of the cerebellar white matter arises from Annexin A7 located in astrocytes and neurites. |
T4314 |
16426-16430 |
RB |
denotes |
Thus |
T4315 |
16481-16487 |
VBZ |
denotes |
arises |
T4316 |
16430-16432 |
, |
denotes |
, |
T4317 |
16432-16435 |
DT |
denotes |
the |
T4318 |
16444-16449 |
NN |
denotes |
stain |
T4319 |
16436-16443 |
JJ |
denotes |
intense |
T4320 |
16450-16452 |
IN |
denotes |
of |
T4321 |
16453-16456 |
DT |
denotes |
the |
T4322 |
16474-16480 |
NN |
denotes |
matter |
T4323 |
16457-16467 |
JJ |
denotes |
cerebellar |
T4324 |
16468-16473 |
JJ |
denotes |
white |
T4325 |
16488-16492 |
IN |
denotes |
from |
T4326 |
16493-16500 |
NN |
denotes |
Annexin |
T4327 |
16501-16503 |
NN |
denotes |
A7 |
T4328 |
16504-16511 |
VBN |
denotes |
located |
T4329 |
16512-16514 |
IN |
denotes |
in |
T4330 |
16515-16525 |
NNS |
denotes |
astrocytes |
T4331 |
16526-16529 |
CC |
denotes |
and |
T4332 |
16530-16538 |
NNS |
denotes |
neurites |
T4333 |
16538-16539 |
. |
denotes |
. |
T4422 |
17691-17701 |
NN |
denotes |
Expression |
T4423 |
17702-17704 |
IN |
denotes |
of |
T4424 |
17705-17712 |
NN |
denotes |
Annexin |
T4425 |
17713-17715 |
NN |
denotes |
A7 |
T4426 |
17716-17718 |
IN |
denotes |
in |
T4427 |
17719-17722 |
DT |
denotes |
the |
T4428 |
17735-17744 |
NN |
denotes |
isocortex |
T4429 |
17723-17728 |
JJ |
denotes |
adult |
T4430 |
17729-17734 |
JJ |
denotes |
human |
T4431 |
17744-17911 |
sentence |
denotes |
In the human parietal neocortex of aged individuals without any neuropathological alterations, subpial astrocytes exhibited a staining of Annexin A7 in the cytoplasm. |
T4432 |
17745-17747 |
IN |
denotes |
In |
T4433 |
17859-17868 |
VBD |
denotes |
exhibited |
T4434 |
17748-17751 |
DT |
denotes |
the |
T4435 |
17767-17776 |
NN |
denotes |
neocortex |
T4436 |
17752-17757 |
JJ |
denotes |
human |
T4437 |
17758-17766 |
JJ |
denotes |
parietal |
T4438 |
17777-17779 |
IN |
denotes |
of |
T4439 |
17780-17784 |
JJ |
denotes |
aged |
T4440 |
17785-17796 |
NNS |
denotes |
individuals |
T4441 |
17797-17804 |
IN |
denotes |
without |
T4442 |
17805-17808 |
DT |
denotes |
any |
T4443 |
17827-17838 |
NNS |
denotes |
alterations |
T4444 |
17809-17826 |
JJ |
denotes |
neuropathological |
T4445 |
17838-17840 |
, |
denotes |
, |
T4446 |
17840-17847 |
JJ |
denotes |
subpial |
T4447 |
17848-17858 |
NNS |
denotes |
astrocytes |
T4448 |
17869-17870 |
DT |
denotes |
a |
T4449 |
17871-17879 |
NN |
denotes |
staining |
T4450 |
17880-17882 |
IN |
denotes |
of |
T4451 |
17883-17890 |
NN |
denotes |
Annexin |
T4452 |
17891-17893 |
NN |
denotes |
A7 |
T4453 |
17894-17896 |
IN |
denotes |
in |
T4454 |
17897-17900 |
DT |
denotes |
the |
T4455 |
17901-17910 |
NN |
denotes |
cytoplasm |
T4456 |
17910-17911 |
. |
denotes |
. |
T4457 |
17911-17988 |
sentence |
denotes |
A nuclear presence of Annexin A7 was limited to single astrocytes (Fig. 6B). |
T4458 |
17912-17913 |
DT |
denotes |
A |
T4459 |
17922-17930 |
NN |
denotes |
presence |
T4460 |
17914-17921 |
JJ |
denotes |
nuclear |
T4461 |
17949-17956 |
VBN |
denotes |
limited |
T4462 |
17931-17933 |
IN |
denotes |
of |
T4463 |
17934-17941 |
NN |
denotes |
Annexin |
T4464 |
17942-17944 |
NN |
denotes |
A7 |
T4465 |
17945-17948 |
VBD |
denotes |
was |
T4466 |
17957-17959 |
IN |
denotes |
to |
T4467 |
17960-17966 |
JJ |
denotes |
single |
T4468 |
17967-17977 |
NNS |
denotes |
astrocytes |
T4469 |
17978-17979 |
-LRB- |
denotes |
( |
T4470 |
17984-17986 |
NN |
denotes |
6B |
T4471 |
17979-17983 |
NN |
denotes |
Fig. |
T4472 |
17986-17987 |
-RRB- |
denotes |
) |
T4473 |
17987-17988 |
. |
denotes |
. |
T4474 |
17988-18149 |
sentence |
denotes |
Pyramidal neurons, predominantly those of layer V exhibited Annexin A7 at the plasma membrane of their perikaryon as well as of the apical dendrite (Fig. 6A,C). |
T4475 |
17989-17998 |
JJ |
denotes |
Pyramidal |
T4476 |
17999-18006 |
NNS |
denotes |
neurons |
T4477 |
18039-18048 |
VBD |
denotes |
exhibited |
T4478 |
18006-18008 |
, |
denotes |
, |
T4479 |
18008-18021 |
RB |
denotes |
predominantly |
T4480 |
18022-18027 |
DT |
denotes |
those |
T4481 |
18028-18030 |
IN |
denotes |
of |
T4482 |
18031-18036 |
NN |
denotes |
layer |
T4483 |
18037-18038 |
CD |
denotes |
V |
T4484 |
18049-18056 |
NN |
denotes |
Annexin |
T4485 |
18057-18059 |
NN |
denotes |
A7 |
T4486 |
18060-18062 |
IN |
denotes |
at |
T4487 |
18063-18066 |
DT |
denotes |
the |
T4488 |
18074-18082 |
NN |
denotes |
membrane |
T4489 |
18067-18073 |
NN |
denotes |
plasma |
T4490 |
18083-18085 |
IN |
denotes |
of |
T4491 |
18086-18091 |
PRP$ |
denotes |
their |
T4492 |
18092-18102 |
NN |
denotes |
perikaryon |
T4493 |
18103-18105 |
RB |
denotes |
as |
T4494 |
18111-18113 |
IN |
denotes |
as |
T4495 |
18106-18110 |
RB |
denotes |
well |
T4496 |
18114-18116 |
IN |
denotes |
of |
T4497 |
18117-18120 |
DT |
denotes |
the |
T4498 |
18128-18136 |
NN |
denotes |
dendrite |
T4499 |
18121-18127 |
JJ |
denotes |
apical |
T4500 |
18137-18138 |
-LRB- |
denotes |
( |
T4501 |
18146-18147 |
NN |
denotes |
C |
T4502 |
18138-18142 |
NN |
denotes |
Fig. |
T4503 |
18143-18145 |
NN |
denotes |
6A |
T4504 |
18145-18146 |
, |
denotes |
, |
T4505 |
18147-18148 |
-RRB- |
denotes |
) |
T4506 |
18148-18149 |
. |
denotes |
. |
T4507 |
18149-18209 |
sentence |
denotes |
The neurons lacked a signal for Annexin A7 in their nuclei. |
T4508 |
18150-18153 |
DT |
denotes |
The |
T4509 |
18154-18161 |
NNS |
denotes |
neurons |
T4510 |
18162-18168 |
VBD |
denotes |
lacked |
T4511 |
18169-18170 |
DT |
denotes |
a |
T4512 |
18171-18177 |
NN |
denotes |
signal |
T4513 |
18178-18181 |
IN |
denotes |
for |
T4514 |
18182-18189 |
NN |
denotes |
Annexin |
T4515 |
18190-18192 |
NN |
denotes |
A7 |
T4516 |
18193-18195 |
IN |
denotes |
in |
T4517 |
18196-18201 |
PRP$ |
denotes |
their |
T4518 |
18202-18208 |
NNS |
denotes |
nuclei |
T4519 |
18208-18209 |
. |
denotes |
. |
T4520 |
18209-18314 |
sentence |
denotes |
Apical dendrites within the molecular layer also indicated a positive staining for Annexin A7 (Fig. 6B). |
T4521 |
18210-18216 |
JJ |
denotes |
Apical |
T4522 |
18217-18226 |
NNS |
denotes |
dendrites |
T4523 |
18259-18268 |
VBD |
denotes |
indicated |
T4524 |
18227-18233 |
IN |
denotes |
within |
T4525 |
18234-18237 |
DT |
denotes |
the |
T4526 |
18248-18253 |
NN |
denotes |
layer |
T4527 |
18238-18247 |
JJ |
denotes |
molecular |
T4528 |
18254-18258 |
RB |
denotes |
also |
T4529 |
18269-18270 |
DT |
denotes |
a |
T4530 |
18280-18288 |
NN |
denotes |
staining |
T4531 |
18271-18279 |
JJ |
denotes |
positive |
T4532 |
18289-18292 |
IN |
denotes |
for |
T4533 |
18293-18300 |
NN |
denotes |
Annexin |
T4534 |
18301-18303 |
NN |
denotes |
A7 |
T4535 |
18304-18305 |
-LRB- |
denotes |
( |
T4536 |
18310-18312 |
NN |
denotes |
6B |
T4537 |
18305-18309 |
NN |
denotes |
Fig. |
T4538 |
18312-18313 |
-RRB- |
denotes |
) |
T4539 |
18313-18314 |
. |
denotes |
. |
T4540 |
18314-18425 |
sentence |
denotes |
The staining pattern of Annexin A7 in the human autopsy brain did not change after pre-treatment with trypsin. |
T4541 |
18315-18318 |
DT |
denotes |
The |
T4542 |
18328-18335 |
NN |
denotes |
pattern |
T4543 |
18319-18327 |
NN |
denotes |
staining |
T4544 |
18385-18391 |
VB |
denotes |
change |
T4545 |
18336-18338 |
IN |
denotes |
of |
T4546 |
18339-18346 |
NN |
denotes |
Annexin |
T4547 |
18347-18349 |
NN |
denotes |
A7 |
T4548 |
18350-18352 |
IN |
denotes |
in |
T4549 |
18353-18356 |
DT |
denotes |
the |
T4550 |
18371-18376 |
NN |
denotes |
brain |
T4551 |
18357-18362 |
JJ |
denotes |
human |
T4552 |
18363-18370 |
NN |
denotes |
autopsy |
T4553 |
18377-18380 |
VBD |
denotes |
did |
T4554 |
18381-18384 |
RB |
denotes |
not |
T4555 |
18392-18397 |
IN |
denotes |
after |
T4556 |
18398-18411 |
NN |
denotes |
pre-treatment |
T4557 |
18412-18416 |
IN |
denotes |
with |
T4558 |
18417-18424 |
NN |
denotes |
trypsin |
T4559 |
18424-18425 |
. |
denotes |
. |
T5136 |
19244-19252 |
NN |
denotes |
Presence |
T5137 |
19253-19255 |
IN |
denotes |
of |
T5138 |
19256-19263 |
NN |
denotes |
Annexin |
T5139 |
19264-19266 |
NN |
denotes |
A7 |
T5140 |
19267-19269 |
IN |
denotes |
in |
T5141 |
19270-19276 |
NNS |
denotes |
nuclei |
T5142 |
19277-19281 |
IN |
denotes |
from |
T5143 |
19282-19290 |
JJ |
denotes |
neuronal |
T5144 |
19306-19311 |
NNS |
denotes |
cells |
T5145 |
19291-19294 |
CC |
denotes |
and |
T5146 |
19295-19305 |
JJ |
denotes |
astroglial |
T5147 |
19311-19547 |
sentence |
denotes |
The nuclear localisation of Annexin A7 in mice observed in immature cells at E16 and differentiated adult neurons could be only detected as a strong signal when the brain sections were pre-treated with trypsin before antibody staining. |
T5148 |
19312-19315 |
DT |
denotes |
The |
T5149 |
19324-19336 |
NN |
denotes |
localisation |
T5150 |
19316-19323 |
JJ |
denotes |
nuclear |
T5151 |
19440-19448 |
VBN |
denotes |
detected |
T5152 |
19337-19339 |
IN |
denotes |
of |
T5153 |
19340-19347 |
NN |
denotes |
Annexin |
T5154 |
19348-19350 |
NN |
denotes |
A7 |
T5155 |
19351-19353 |
IN |
denotes |
in |
T5156 |
19354-19358 |
NNS |
denotes |
mice |
T5157 |
19359-19367 |
VBN |
denotes |
observed |
T5158 |
19368-19370 |
IN |
denotes |
in |
T5159 |
19371-19379 |
JJ |
denotes |
immature |
T5160 |
19380-19385 |
NNS |
denotes |
cells |
T5161 |
19386-19388 |
IN |
denotes |
at |
T5162 |
19389-19392 |
NN |
denotes |
E16 |
T5163 |
19393-19396 |
CC |
denotes |
and |
T5164 |
19397-19411 |
VBN |
denotes |
differentiated |
T5165 |
19418-19425 |
NNS |
denotes |
neurons |
T5166 |
19412-19417 |
JJ |
denotes |
adult |
T5167 |
19426-19431 |
MD |
denotes |
could |
T5168 |
19432-19434 |
VB |
denotes |
be |
T5169 |
19435-19439 |
RB |
denotes |
only |
T5170 |
19449-19451 |
IN |
denotes |
as |
T5171 |
19452-19453 |
DT |
denotes |
a |
T5172 |
19461-19467 |
NN |
denotes |
signal |
T5173 |
19454-19460 |
JJ |
denotes |
strong |
T5174 |
19468-19472 |
WRB |
denotes |
when |
T5175 |
19497-19508 |
VBN |
denotes |
pre-treated |
T5176 |
19473-19476 |
DT |
denotes |
the |
T5177 |
19483-19491 |
NNS |
denotes |
sections |
T5178 |
19477-19482 |
NN |
denotes |
brain |
T5179 |
19492-19496 |
VBD |
denotes |
were |
T5180 |
19509-19513 |
IN |
denotes |
with |
T5181 |
19514-19521 |
NN |
denotes |
trypsin |
T5182 |
19522-19528 |
IN |
denotes |
before |
T5183 |
19529-19537 |
NN |
denotes |
antibody |
T5184 |
19538-19546 |
NN |
denotes |
staining |
T5185 |
19546-19547 |
. |
denotes |
. |
T5186 |
19547-19653 |
sentence |
denotes |
This procedure is thought to allow the antibodies to access epitopes masked by the formaldehyde fixation. |
T5187 |
19548-19552 |
DT |
denotes |
This |
T5188 |
19553-19562 |
NN |
denotes |
procedure |
T5189 |
19566-19573 |
VBN |
denotes |
thought |
T5190 |
19563-19565 |
VBZ |
denotes |
is |
T5191 |
19574-19576 |
TO |
denotes |
to |
T5192 |
19577-19582 |
VB |
denotes |
allow |
T5193 |
19583-19586 |
DT |
denotes |
the |
T5194 |
19587-19597 |
NNS |
denotes |
antibodies |
T5195 |
19601-19607 |
VB |
denotes |
access |
T5196 |
19598-19600 |
TO |
denotes |
to |
T5197 |
19608-19616 |
NNS |
denotes |
epitopes |
T5198 |
19617-19623 |
VBN |
denotes |
masked |
T5199 |
19624-19626 |
IN |
denotes |
by |
T5200 |
19627-19630 |
DT |
denotes |
the |
T5201 |
19644-19652 |
NN |
denotes |
fixation |
T5202 |
19631-19643 |
NN |
denotes |
formaldehyde |
T5203 |
19652-19653 |
. |
denotes |
. |
T5204 |
19653-19718 |
sentence |
denotes |
Methanol fixation of cultured cells led to inconsistent results. |
T5205 |
19654-19662 |
NN |
denotes |
Methanol |
T5206 |
19663-19671 |
NN |
denotes |
fixation |
T5207 |
19690-19693 |
VBD |
denotes |
led |
T5208 |
19672-19674 |
IN |
denotes |
of |
T5209 |
19675-19683 |
VBN |
denotes |
cultured |
T5210 |
19684-19689 |
NNS |
denotes |
cells |
T5211 |
19694-19696 |
IN |
denotes |
to |
T5212 |
19697-19709 |
JJ |
denotes |
inconsistent |
T5213 |
19710-19717 |
NNS |
denotes |
results |
T5214 |
19717-19718 |
. |
denotes |
. |
T5215 |
19718-19929 |
sentence |
denotes |
Antigen retrieval in formalin-fixed and paraffin-embedded tissue sections employs various heating or proteolytic pre-treatment methods [[15-17]; Ein Handbuch für die Histologie, dianova GmbH, Hamburg, Germany]. |
T5216 |
19719-19726 |
NN |
denotes |
Antigen |
T5217 |
19727-19736 |
NN |
denotes |
retrieval |
T5218 |
19793-19800 |
VBZ |
denotes |
employs |
T5219 |
19737-19739 |
IN |
denotes |
in |
T5220 |
19740-19748 |
NN |
denotes |
formalin |
T5221 |
19749-19754 |
VBN |
denotes |
fixed |
T5222 |
19748-19749 |
HYPH |
denotes |
- |
T5223 |
19784-19792 |
NNS |
denotes |
sections |
T5224 |
19755-19758 |
CC |
denotes |
and |
T5225 |
19759-19767 |
NN |
denotes |
paraffin |
T5226 |
19768-19776 |
VBN |
denotes |
embedded |
T5227 |
19767-19768 |
HYPH |
denotes |
- |
T5228 |
19777-19783 |
NN |
denotes |
tissue |
T5229 |
19801-19808 |
JJ |
denotes |
various |
T5230 |
19846-19853 |
NNS |
denotes |
methods |
T5231 |
19809-19816 |
NN |
denotes |
heating |
T5232 |
19817-19819 |
CC |
denotes |
or |
T5233 |
19820-19831 |
JJ |
denotes |
proteolytic |
T5234 |
19832-19845 |
NN |
denotes |
pre-treatment |
T5235 |
19854-19855 |
-LRB- |
denotes |
[ |
T5236 |
19855-19856 |
-LRB- |
denotes |
[ |
T5237 |
19856-19858 |
CD |
denotes |
15 |
T5238 |
19858-19859 |
SYM |
denotes |
- |
T5239 |
19859-19861 |
CD |
denotes |
17 |
T5240 |
19861-19862 |
-RRB- |
denotes |
] |
T5241 |
19862-19863 |
: |
denotes |
; |
T5242 |
19864-19867 |
NNP |
denotes |
Ein |
T5243 |
19868-19876 |
NNP |
denotes |
Handbuch |
T5244 |
19877-19880 |
NNP |
denotes |
für |
T5245 |
19881-19884 |
NNP |
denotes |
die |
T5246 |
19885-19895 |
NNP |
denotes |
Histologie |
T5247 |
19895-19897 |
, |
denotes |
, |
T5248 |
19897-19904 |
NNP |
denotes |
dianova |
T5249 |
19905-19909 |
NNP |
denotes |
GmbH |
T5250 |
19909-19911 |
, |
denotes |
, |
T5251 |
19911-19918 |
NNP |
denotes |
Hamburg |
T5252 |
19918-19920 |
, |
denotes |
, |
T5253 |
19920-19927 |
NNP |
denotes |
Germany |
T5254 |
19927-19928 |
-RRB- |
denotes |
] |
T5255 |
19928-19929 |
. |
denotes |
. |
T5256 |
19929-20064 |
sentence |
denotes |
These methods can result in moderate or strong specific antibody staining, but the detectability of other antigens might be decreased. |
T5257 |
19930-19935 |
DT |
denotes |
These |
T5258 |
19936-19943 |
NNS |
denotes |
methods |
T5259 |
19948-19954 |
VB |
denotes |
result |
T5260 |
19944-19947 |
MD |
denotes |
can |
T5261 |
19955-19957 |
IN |
denotes |
in |
T5262 |
19958-19966 |
JJ |
denotes |
moderate |
T5263 |
19995-20003 |
NN |
denotes |
staining |
T5264 |
19967-19969 |
CC |
denotes |
or |
T5265 |
19970-19976 |
JJ |
denotes |
strong |
T5266 |
19977-19985 |
JJ |
denotes |
specific |
T5267 |
19986-19994 |
NN |
denotes |
antibody |
T5268 |
20003-20005 |
, |
denotes |
, |
T5269 |
20005-20008 |
CC |
denotes |
but |
T5270 |
20009-20012 |
DT |
denotes |
the |
T5271 |
20013-20026 |
NN |
denotes |
detectability |
T5272 |
20054-20063 |
VBN |
denotes |
decreased |
T5273 |
20027-20029 |
IN |
denotes |
of |
T5274 |
20030-20035 |
JJ |
denotes |
other |
T5275 |
20036-20044 |
NNS |
denotes |
antigens |
T5276 |
20045-20050 |
MD |
denotes |
might |
T5277 |
20051-20053 |
VB |
denotes |
be |
T5278 |
20063-20064 |
. |
denotes |
. |
T5279 |
20064-20143 |
sentence |
denotes |
Moreover, the optimal pre-treatment has to be individualised for each antigen. |
T5280 |
20065-20073 |
RB |
denotes |
Moreover |
T5281 |
20101-20104 |
VBZ |
denotes |
has |
T5282 |
20073-20075 |
, |
denotes |
, |
T5283 |
20075-20078 |
DT |
denotes |
the |
T5284 |
20087-20100 |
NN |
denotes |
pre-treatment |
T5285 |
20079-20086 |
JJ |
denotes |
optimal |
T5286 |
20105-20107 |
TO |
denotes |
to |
T5287 |
20111-20125 |
VBN |
denotes |
individualised |
T5288 |
20108-20110 |
VB |
denotes |
be |
T5289 |
20126-20129 |
IN |
denotes |
for |
T5290 |
20130-20134 |
DT |
denotes |
each |
T5291 |
20135-20142 |
NN |
denotes |
antigen |
T5292 |
20142-20143 |
. |
denotes |
. |
T5293 |
20143-20377 |
sentence |
denotes |
To verify the presence of endogenous Annexin A7 in nuclei, we used a biochemical approach and purified nuclei from neuro-2a, PC-12, and C6 cells and treated them with a hypotonic extraction buffer to obtain the nucleoplasm (Fig. 7A). |
T5294 |
20144-20146 |
TO |
denotes |
To |
T5295 |
20147-20153 |
VB |
denotes |
verify |
T5296 |
20206-20210 |
VBD |
denotes |
used |
T5297 |
20154-20157 |
DT |
denotes |
the |
T5298 |
20158-20166 |
NN |
denotes |
presence |
T5299 |
20167-20169 |
IN |
denotes |
of |
T5300 |
20170-20180 |
JJ |
denotes |
endogenous |
T5301 |
20189-20191 |
NN |
denotes |
A7 |
T5302 |
20181-20188 |
NN |
denotes |
Annexin |
T5303 |
20192-20194 |
IN |
denotes |
in |
T5304 |
20195-20201 |
NNS |
denotes |
nuclei |
T5305 |
20201-20203 |
, |
denotes |
, |
T5306 |
20203-20205 |
PRP |
denotes |
we |
T5307 |
20211-20212 |
DT |
denotes |
a |
T5308 |
20225-20233 |
NN |
denotes |
approach |
T5309 |
20213-20224 |
JJ |
denotes |
biochemical |
T5310 |
20234-20237 |
CC |
denotes |
and |
T5311 |
20238-20246 |
VBN |
denotes |
purified |
T5312 |
20247-20253 |
NNS |
denotes |
nuclei |
T5313 |
20254-20258 |
IN |
denotes |
from |
T5314 |
20259-20264 |
NN |
denotes |
neuro |
T5315 |
20283-20288 |
NNS |
denotes |
cells |
T5316 |
20264-20265 |
HYPH |
denotes |
- |
T5317 |
20265-20267 |
CD |
denotes |
2a |
T5318 |
20267-20269 |
, |
denotes |
, |
T5319 |
20269-20271 |
NN |
denotes |
PC |
T5320 |
20271-20272 |
HYPH |
denotes |
- |
T5321 |
20272-20274 |
CD |
denotes |
12 |
T5322 |
20274-20276 |
, |
denotes |
, |
T5323 |
20276-20279 |
CC |
denotes |
and |
T5324 |
20280-20282 |
NN |
denotes |
C6 |
T5325 |
20289-20292 |
CC |
denotes |
and |
T5326 |
20293-20300 |
VBN |
denotes |
treated |
T5327 |
20301-20305 |
PRP |
denotes |
them |
T5328 |
20306-20310 |
IN |
denotes |
with |
T5329 |
20311-20312 |
DT |
denotes |
a |
T5330 |
20334-20340 |
NN |
denotes |
buffer |
T5331 |
20313-20322 |
JJ |
denotes |
hypotonic |
T5332 |
20323-20333 |
NN |
denotes |
extraction |
T5333 |
20341-20343 |
TO |
denotes |
to |
T5334 |
20344-20350 |
VB |
denotes |
obtain |
T5335 |
20351-20354 |
DT |
denotes |
the |
T5336 |
20355-20366 |
NN |
denotes |
nucleoplasm |
T5337 |
20367-20368 |
-LRB- |
denotes |
( |
T5338 |
20373-20375 |
NN |
denotes |
7A |
T5339 |
20368-20372 |
NN |
denotes |
Fig. |
T5340 |
20375-20376 |
-RRB- |
denotes |
) |
T5341 |
20376-20377 |
. |
denotes |
. |
T5342 |
20377-20525 |
sentence |
denotes |
The nucleoplasm and the remaining nuclei, from which the nucleoplasm has been extracted partially, were subjected to SDS-PAGE and Western blotting. |
T5343 |
20378-20381 |
DT |
denotes |
The |
T5344 |
20382-20393 |
NN |
denotes |
nucleoplasm |
T5345 |
20482-20491 |
VBN |
denotes |
subjected |
T5346 |
20394-20397 |
CC |
denotes |
and |
T5347 |
20398-20401 |
DT |
denotes |
the |
T5348 |
20412-20418 |
NNS |
denotes |
nuclei |
T5349 |
20402-20411 |
VBG |
denotes |
remaining |
T5350 |
20418-20420 |
, |
denotes |
, |
T5351 |
20420-20424 |
IN |
denotes |
from |
T5352 |
20456-20465 |
VBN |
denotes |
extracted |
T5353 |
20425-20430 |
WDT |
denotes |
which |
T5354 |
20431-20434 |
DT |
denotes |
the |
T5355 |
20435-20446 |
NN |
denotes |
nucleoplasm |
T5356 |
20447-20450 |
VBZ |
denotes |
has |
T5357 |
20451-20455 |
VBN |
denotes |
been |
T5358 |
20466-20475 |
RB |
denotes |
partially |
T5359 |
20475-20477 |
, |
denotes |
, |
T5360 |
20477-20481 |
VBD |
denotes |
were |
T5361 |
20492-20494 |
IN |
denotes |
to |
T5362 |
20495-20498 |
NN |
denotes |
SDS |
T5363 |
20499-20503 |
NN |
denotes |
PAGE |
T5364 |
20498-20499 |
HYPH |
denotes |
- |
T5365 |
20504-20507 |
CC |
denotes |
and |
T5366 |
20508-20515 |
NNP |
denotes |
Western |
T5367 |
20516-20524 |
NN |
denotes |
blotting |
T5368 |
20524-20525 |
. |
denotes |
. |
T5369 |
20525-20763 |
sentence |
denotes |
Western blots probed with mAb 203–217 showed Annexin A7 in the nucleoplasm of neuronal (neuro-2a, PC-12) and astroglial (C6) cell lines (Fig. 7A), however, the large isoform could only be clearly extracted with nucleoplasm from C6 cells. |
T5370 |
20526-20533 |
NNP |
denotes |
Western |
T5371 |
20534-20539 |
NNS |
denotes |
blots |
T5372 |
20564-20570 |
VBD |
denotes |
showed |
T5373 |
20540-20546 |
VBN |
denotes |
probed |
T5374 |
20547-20551 |
IN |
denotes |
with |
T5375 |
20552-20555 |
NN |
denotes |
mAb |
T5376 |
20556-20559 |
CD |
denotes |
203 |
T5377 |
20559-20560 |
SYM |
denotes |
– |
T5378 |
20560-20563 |
CD |
denotes |
217 |
T5379 |
20722-20731 |
VBN |
denotes |
extracted |
T5380 |
20571-20578 |
NN |
denotes |
Annexin |
T5381 |
20579-20581 |
NN |
denotes |
A7 |
T5382 |
20582-20584 |
IN |
denotes |
in |
T5383 |
20585-20588 |
DT |
denotes |
the |
T5384 |
20589-20600 |
NN |
denotes |
nucleoplasm |
T5385 |
20601-20603 |
IN |
denotes |
of |
T5386 |
20604-20612 |
JJ |
denotes |
neuronal |
T5387 |
20656-20661 |
NNS |
denotes |
lines |
T5388 |
20613-20614 |
-LRB- |
denotes |
( |
T5389 |
20620-20622 |
NN |
denotes |
2a |
T5390 |
20614-20619 |
NN |
denotes |
neuro |
T5391 |
20619-20620 |
HYPH |
denotes |
- |
T5392 |
20622-20624 |
, |
denotes |
, |
T5393 |
20624-20626 |
NN |
denotes |
PC |
T5394 |
20626-20627 |
HYPH |
denotes |
- |
T5395 |
20627-20629 |
CD |
denotes |
12 |
T5396 |
20629-20630 |
-RRB- |
denotes |
) |
T5397 |
20631-20634 |
CC |
denotes |
and |
T5398 |
20635-20645 |
JJ |
denotes |
astroglial |
T5399 |
20646-20647 |
-LRB- |
denotes |
( |
T5400 |
20647-20649 |
NN |
denotes |
C6 |
T5401 |
20649-20650 |
-RRB- |
denotes |
) |
T5402 |
20651-20655 |
NN |
denotes |
cell |
T5403 |
20662-20663 |
-LRB- |
denotes |
( |
T5404 |
20668-20670 |
NN |
denotes |
7A |
T5405 |
20663-20667 |
NN |
denotes |
Fig. |
T5406 |
20670-20671 |
-RRB- |
denotes |
) |
T5407 |
20671-20673 |
, |
denotes |
, |
T5408 |
20673-20680 |
RB |
denotes |
however |
T5409 |
20680-20682 |
, |
denotes |
, |
T5410 |
20682-20685 |
DT |
denotes |
the |
T5411 |
20692-20699 |
NN |
denotes |
isoform |
T5412 |
20686-20691 |
JJ |
denotes |
large |
T5413 |
20700-20705 |
MD |
denotes |
could |
T5414 |
20706-20710 |
RB |
denotes |
only |
T5415 |
20711-20713 |
VB |
denotes |
be |
T5416 |
20714-20721 |
RB |
denotes |
clearly |
T5417 |
20732-20736 |
IN |
denotes |
with |
T5418 |
20737-20748 |
NN |
denotes |
nucleoplasm |
T5419 |
20749-20753 |
IN |
denotes |
from |
T5420 |
20754-20756 |
NN |
denotes |
C6 |
T5421 |
20757-20762 |
NNS |
denotes |
cells |
T5422 |
20762-20763 |
. |
denotes |
. |
T5423 |
20763-21047 |
sentence |
denotes |
For control, antibodies against LAP2α, Emerin and tubulin were used to verify that the nucleoplasm (marker:LAP2α, which is anon-membrane-bound isoform of LAP2) was successfully extracted and also not contaminated by nuclear membranes (marker:Emerin) or by cytoplasm (marker:tubulin). |
T5424 |
20764-20767 |
IN |
denotes |
For |
T5425 |
20827-20831 |
VBN |
denotes |
used |
T5426 |
20768-20775 |
NN |
denotes |
control |
T5427 |
20775-20777 |
, |
denotes |
, |
T5428 |
20777-20787 |
NNS |
denotes |
antibodies |
T5429 |
20788-20795 |
IN |
denotes |
against |
T5430 |
20796-20801 |
NN |
denotes |
LAP2α |
T5431 |
20801-20803 |
, |
denotes |
, |
T5432 |
20803-20809 |
NN |
denotes |
Emerin |
T5433 |
20810-20813 |
CC |
denotes |
and |
T5434 |
20814-20821 |
NN |
denotes |
tubulin |
T5435 |
20822-20826 |
VBD |
denotes |
were |
T5436 |
20832-20834 |
TO |
denotes |
to |
T5437 |
20835-20841 |
VB |
denotes |
verify |
T5438 |
20842-20846 |
IN |
denotes |
that |
T5439 |
20941-20950 |
VBN |
denotes |
extracted |
T5440 |
20847-20850 |
DT |
denotes |
the |
T5441 |
20851-20862 |
NN |
denotes |
nucleoplasm |
T5442 |
20863-20864 |
-LRB- |
denotes |
( |
T5443 |
20871-20876 |
NN |
denotes |
LAP2α |
T5444 |
20864-20870 |
NN |
denotes |
marker |
T5445 |
20870-20871 |
: |
denotes |
: |
T5446 |
20876-20878 |
, |
denotes |
, |
T5447 |
20878-20883 |
WDT |
denotes |
which |
T5448 |
20884-20886 |
VBZ |
denotes |
is |
T5449 |
20887-20891 |
NN |
denotes |
anon |
T5450 |
20892-20900 |
NN |
denotes |
membrane |
T5451 |
20891-20892 |
HYPH |
denotes |
- |
T5452 |
20901-20906 |
VBN |
denotes |
bound |
T5453 |
20900-20901 |
HYPH |
denotes |
- |
T5454 |
20907-20914 |
NN |
denotes |
isoform |
T5455 |
20915-20917 |
IN |
denotes |
of |
T5456 |
20918-20922 |
NN |
denotes |
LAP2 |
T5457 |
20922-20923 |
-RRB- |
denotes |
) |
T5458 |
20924-20927 |
VBD |
denotes |
was |
T5459 |
20928-20940 |
RB |
denotes |
successfully |
T5460 |
20951-20954 |
CC |
denotes |
and |
T5461 |
20955-20959 |
RB |
denotes |
also |
T5462 |
20960-20963 |
RB |
denotes |
not |
T5463 |
20964-20976 |
VBN |
denotes |
contaminated |
T5464 |
20977-20979 |
IN |
denotes |
by |
T5465 |
20980-20987 |
JJ |
denotes |
nuclear |
T5466 |
20988-20997 |
NNS |
denotes |
membranes |
T5467 |
20998-20999 |
-LRB- |
denotes |
( |
T5468 |
21006-21012 |
NN |
denotes |
Emerin |
T5469 |
20999-21005 |
NN |
denotes |
marker |
T5470 |
21005-21006 |
: |
denotes |
: |
T5471 |
21012-21013 |
-RRB- |
denotes |
) |
T5472 |
21014-21016 |
CC |
denotes |
or |
T5473 |
21017-21019 |
IN |
denotes |
by |
T5474 |
21020-21029 |
NN |
denotes |
cytoplasm |
T5475 |
21030-21031 |
-LRB- |
denotes |
( |
T5476 |
21038-21045 |
NN |
denotes |
tubulin |
T5477 |
21031-21037 |
NN |
denotes |
marker |
T5478 |
21037-21038 |
: |
denotes |
: |
T5479 |
21045-21046 |
-RRB- |
denotes |
) |
T5480 |
21046-21047 |
. |
denotes |
. |
T5481 |
21047-21197 |
sentence |
denotes |
Likewise, in these neuronal and astroglial cell lines Annexin A7 had been observed in the nucleus by immunofluorescence (Fig. 7B and data not shown). |
T5482 |
21048-21056 |
RB |
denotes |
Likewise |
T5483 |
21122-21130 |
VBN |
denotes |
observed |
T5484 |
21056-21058 |
, |
denotes |
, |
T5485 |
21058-21060 |
IN |
denotes |
in |
T5486 |
21061-21066 |
DT |
denotes |
these |
T5487 |
21096-21101 |
NNS |
denotes |
lines |
T5488 |
21067-21075 |
JJ |
denotes |
neuronal |
T5489 |
21076-21079 |
CC |
denotes |
and |
T5490 |
21080-21090 |
JJ |
denotes |
astroglial |
T5491 |
21091-21095 |
NN |
denotes |
cell |
T5492 |
21102-21109 |
NN |
denotes |
Annexin |
T5493 |
21110-21112 |
NN |
denotes |
A7 |
T5494 |
21113-21116 |
VBD |
denotes |
had |
T5495 |
21117-21121 |
VBN |
denotes |
been |
T5496 |
21131-21133 |
IN |
denotes |
in |
T5497 |
21134-21137 |
DT |
denotes |
the |
T5498 |
21138-21145 |
NN |
denotes |
nucleus |
T5499 |
21146-21148 |
IN |
denotes |
by |
T5500 |
21149-21167 |
NN |
denotes |
immunofluorescence |
T5501 |
21168-21169 |
-LRB- |
denotes |
( |
T5502 |
21174-21176 |
NN |
denotes |
7B |
T5503 |
21169-21173 |
NN |
denotes |
Fig. |
T5504 |
21177-21180 |
CC |
denotes |
and |
T5505 |
21181-21185 |
NNS |
denotes |
data |
T5506 |
21190-21195 |
VBN |
denotes |
shown |
T5507 |
21186-21189 |
RB |
denotes |
not |
T5508 |
21195-21196 |
-RRB- |
denotes |
) |
T5509 |
21196-21197 |
. |
denotes |
. |
T5510 |
21197-21374 |
sentence |
denotes |
We noticed no difference between PC-12 and neuro-2a cells, however, as for the brain sections, we found an increase of the Annexin A7 staining after pre-treatment with trypsin. |
T5511 |
21198-21200 |
PRP |
denotes |
We |
T5512 |
21201-21208 |
VBD |
denotes |
noticed |
T5513 |
21296-21301 |
VBD |
denotes |
found |
T5514 |
21209-21211 |
DT |
denotes |
no |
T5515 |
21212-21222 |
NN |
denotes |
difference |
T5516 |
21223-21230 |
IN |
denotes |
between |
T5517 |
21231-21233 |
NN |
denotes |
PC |
T5518 |
21250-21255 |
NNS |
denotes |
cells |
T5519 |
21233-21234 |
HYPH |
denotes |
- |
T5520 |
21234-21236 |
CD |
denotes |
12 |
T5521 |
21237-21240 |
CC |
denotes |
and |
T5522 |
21241-21246 |
NN |
denotes |
neuro |
T5523 |
21247-21249 |
NN |
denotes |
2a |
T5524 |
21246-21247 |
HYPH |
denotes |
- |
T5525 |
21255-21257 |
, |
denotes |
, |
T5526 |
21257-21264 |
RB |
denotes |
however |
T5527 |
21264-21266 |
, |
denotes |
, |
T5528 |
21266-21268 |
IN |
denotes |
as |
T5529 |
21269-21272 |
IN |
denotes |
for |
T5530 |
21273-21276 |
DT |
denotes |
the |
T5531 |
21283-21291 |
NNS |
denotes |
sections |
T5532 |
21277-21282 |
NN |
denotes |
brain |
T5533 |
21291-21293 |
, |
denotes |
, |
T5534 |
21293-21295 |
PRP |
denotes |
we |
T5535 |
21302-21304 |
DT |
denotes |
an |
T5536 |
21305-21313 |
NN |
denotes |
increase |
T5537 |
21314-21316 |
IN |
denotes |
of |
T5538 |
21317-21320 |
DT |
denotes |
the |
T5539 |
21332-21340 |
NN |
denotes |
staining |
T5540 |
21321-21328 |
NN |
denotes |
Annexin |
T5541 |
21329-21331 |
NN |
denotes |
A7 |
T5542 |
21341-21346 |
IN |
denotes |
after |
T5543 |
21347-21360 |
NN |
denotes |
pre-treatment |
T5544 |
21361-21365 |
IN |
denotes |
with |
T5545 |
21366-21373 |
NN |
denotes |
trypsin |
T5546 |
21373-21374 |
. |
denotes |
. |
T6416 |
22360-22362 |
IN |
denotes |
In |
T6417 |
22384-22392 |
VBD |
denotes |
explored |
T6418 |
22363-22366 |
DT |
denotes |
the |
T6419 |
22375-22380 |
NN |
denotes |
study |
T6420 |
22367-22374 |
JJ |
denotes |
present |
T6421 |
22381-22383 |
PRP |
denotes |
we |
T6422 |
22393-22396 |
DT |
denotes |
the |
T6423 |
22397-22407 |
NN |
denotes |
appearance |
T6424 |
22408-22410 |
IN |
denotes |
of |
T6425 |
22411-22418 |
NN |
denotes |
Annexin |
T6426 |
22419-22421 |
NN |
denotes |
A7 |
T6427 |
22422-22428 |
IN |
denotes |
during |
T6428 |
22429-22434 |
NN |
denotes |
mouse |
T6429 |
22435-22446 |
NN |
denotes |
development |
T6430 |
22447-22449 |
IN |
denotes |
at |
T6431 |
22450-22453 |
DT |
denotes |
the |
T6432 |
22471-22476 |
NN |
denotes |
level |
T6433 |
22454-22458 |
NN |
denotes |
mRNA |
T6434 |
22459-22462 |
CC |
denotes |
and |
T6435 |
22463-22470 |
NN |
denotes |
protein |
T6436 |
22477-22480 |
CC |
denotes |
and |
T6437 |
22481-22488 |
VBD |
denotes |
focused |
T6438 |
22489-22491 |
IN |
denotes |
on |
T6439 |
22492-22495 |
DT |
denotes |
the |
T6440 |
22512-22518 |
NN |
denotes |
system |
T6441 |
22496-22503 |
JJ |
denotes |
central |
T6442 |
22504-22511 |
JJ |
denotes |
nervous |
T6443 |
22519-22525 |
IN |
denotes |
during |
T6444 |
22526-22539 |
NN |
denotes |
embryogenesis |
T6445 |
22539-22540 |
. |
denotes |
. |
T6446 |
22540-22689 |
sentence |
denotes |
Northern blot and immunohistochemistry analysis show the expression of Annexin A7 in all embryonic tissues from day E5 on, the earliest day studied. |
T6447 |
22541-22549 |
NNP |
denotes |
Northern |
T6448 |
22550-22554 |
NN |
denotes |
blot |
T6449 |
22580-22588 |
NN |
denotes |
analysis |
T6450 |
22555-22558 |
CC |
denotes |
and |
T6451 |
22559-22579 |
NN |
denotes |
immunohistochemistry |
T6452 |
22589-22593 |
VBP |
denotes |
show |
T6453 |
22594-22597 |
DT |
denotes |
the |
T6454 |
22598-22608 |
NN |
denotes |
expression |
T6455 |
22609-22611 |
IN |
denotes |
of |
T6456 |
22612-22619 |
NN |
denotes |
Annexin |
T6457 |
22620-22622 |
NN |
denotes |
A7 |
T6458 |
22623-22625 |
IN |
denotes |
in |
T6459 |
22626-22629 |
DT |
denotes |
all |
T6460 |
22640-22647 |
NNS |
denotes |
tissues |
T6461 |
22630-22639 |
JJ |
denotes |
embryonic |
T6462 |
22648-22652 |
IN |
denotes |
from |
T6463 |
22653-22656 |
NN |
denotes |
day |
T6464 |
22657-22659 |
NN |
denotes |
E5 |
T6465 |
22660-22662 |
RB |
denotes |
on |
T6466 |
22662-22664 |
, |
denotes |
, |
T6467 |
22664-22667 |
DT |
denotes |
the |
T6468 |
22677-22680 |
NN |
denotes |
day |
T6469 |
22668-22676 |
JJS |
denotes |
earliest |
T6470 |
22681-22688 |
VBN |
denotes |
studied |
T6471 |
22688-22689 |
. |
denotes |
. |
T6472 |
22689-22860 |
sentence |
denotes |
Differentiating cells, like the neural ectoderm, which is the origin of the cells belonging to the nervous system, show Annexin A7 immunoreactivity mainly in the cytosol. |
T6473 |
22690-22705 |
VBG |
denotes |
Differentiating |
T6474 |
22706-22711 |
NNS |
denotes |
cells |
T6475 |
22805-22809 |
VBP |
denotes |
show |
T6476 |
22711-22713 |
, |
denotes |
, |
T6477 |
22713-22717 |
IN |
denotes |
like |
T6478 |
22718-22721 |
DT |
denotes |
the |
T6479 |
22729-22737 |
NN |
denotes |
ectoderm |
T6480 |
22722-22728 |
JJ |
denotes |
neural |
T6481 |
22737-22739 |
, |
denotes |
, |
T6482 |
22739-22744 |
WDT |
denotes |
which |
T6483 |
22745-22747 |
VBZ |
denotes |
is |
T6484 |
22748-22751 |
DT |
denotes |
the |
T6485 |
22752-22758 |
NN |
denotes |
origin |
T6486 |
22759-22761 |
IN |
denotes |
of |
T6487 |
22762-22765 |
DT |
denotes |
the |
T6488 |
22766-22771 |
NNS |
denotes |
cells |
T6489 |
22772-22781 |
VBG |
denotes |
belonging |
T6490 |
22782-22784 |
IN |
denotes |
to |
T6491 |
22785-22788 |
DT |
denotes |
the |
T6492 |
22797-22803 |
NN |
denotes |
system |
T6493 |
22789-22796 |
JJ |
denotes |
nervous |
T6494 |
22803-22805 |
, |
denotes |
, |
T6495 |
22810-22817 |
NN |
denotes |
Annexin |
T6496 |
22818-22820 |
NN |
denotes |
A7 |
T6497 |
22821-22837 |
NN |
denotes |
immunoreactivity |
T6498 |
22838-22844 |
RB |
denotes |
mainly |
T6499 |
22845-22847 |
IN |
denotes |
in |
T6500 |
22848-22851 |
DT |
denotes |
the |
T6501 |
22852-22859 |
NN |
denotes |
cytosol |
T6502 |
22859-22860 |
. |
denotes |
. |
T6503 |
22860-22958 |
sentence |
denotes |
Endodermal as well as mesodermal cells exhibit a similar subcellular localization of the protein. |
T6505 |
22894-22899 |
NNS |
denotes |
cells |
T6506 |
22872-22874 |
RB |
denotes |
as |
T6507 |
22880-22882 |
IN |
denotes |
as |
T6508 |
22875-22879 |
RB |
denotes |
well |
T6509 |
22883-22893 |
JJ |
denotes |
mesodermal |
T6510 |
22900-22907 |
VBP |
denotes |
exhibit |
T6511 |
22908-22909 |
DT |
denotes |
a |
T6512 |
22930-22942 |
NN |
denotes |
localization |
T6513 |
22910-22917 |
JJ |
denotes |
similar |
T6514 |
22918-22929 |
JJ |
denotes |
subcellular |
T6515 |
22943-22945 |
IN |
denotes |
of |
T6516 |
22946-22949 |
DT |
denotes |
the |
T6517 |
22950-22957 |
NN |
denotes |
protein |
T6518 |
22957-22958 |
. |
denotes |
. |
T6519 |
22958-23056 |
sentence |
denotes |
In the developing brain we noted a striking change in the subcellular distribution of Annexin A7. |
T6520 |
22959-22961 |
IN |
denotes |
In |
T6521 |
22986-22991 |
VBD |
denotes |
noted |
T6522 |
22962-22965 |
DT |
denotes |
the |
T6523 |
22977-22982 |
NN |
denotes |
brain |
T6524 |
22966-22976 |
VBG |
denotes |
developing |
T6525 |
22983-22985 |
PRP |
denotes |
we |
T6526 |
22992-22993 |
DT |
denotes |
a |
T6527 |
23003-23009 |
NN |
denotes |
change |
T6528 |
22994-23002 |
JJ |
denotes |
striking |
T6529 |
23010-23012 |
IN |
denotes |
in |
T6530 |
23013-23016 |
DT |
denotes |
the |
T6531 |
23029-23041 |
NN |
denotes |
distribution |
T6532 |
23017-23028 |
JJ |
denotes |
subcellular |
T6533 |
23042-23044 |
IN |
denotes |
of |
T6534 |
23045-23052 |
NN |
denotes |
Annexin |
T6535 |
23053-23055 |
NN |
denotes |
A7 |
T6536 |
23055-23056 |
. |
denotes |
. |
T6537 |
23056-23224 |
sentence |
denotes |
Cells in the stratum germinativum of the neopallial cortex, which surrounds the lateral ventricle, show at E13 and E15 a staining for Annexin A7 mainly in the cytosol. |
T6538 |
23057-23062 |
NNS |
denotes |
Cells |
T6539 |
23156-23160 |
VBP |
denotes |
show |
T6540 |
23063-23065 |
IN |
denotes |
in |
T6541 |
23066-23069 |
DT |
denotes |
the |
T6542 |
23078-23090 |
NN |
denotes |
germinativum |
T6543 |
23070-23077 |
NN |
denotes |
stratum |
T6544 |
23091-23093 |
IN |
denotes |
of |
T6545 |
23094-23097 |
DT |
denotes |
the |
T6546 |
23109-23115 |
NN |
denotes |
cortex |
T6547 |
23098-23108 |
JJ |
denotes |
neopallial |
T6548 |
23115-23117 |
, |
denotes |
, |
T6549 |
23117-23122 |
WDT |
denotes |
which |
T6550 |
23123-23132 |
VBZ |
denotes |
surrounds |
T6551 |
23133-23136 |
DT |
denotes |
the |
T6552 |
23145-23154 |
NN |
denotes |
ventricle |
T6553 |
23137-23144 |
JJ |
denotes |
lateral |
T6554 |
23154-23156 |
, |
denotes |
, |
T6555 |
23161-23163 |
IN |
denotes |
at |
T6556 |
23164-23167 |
NN |
denotes |
E13 |
T6557 |
23168-23171 |
CC |
denotes |
and |
T6558 |
23172-23175 |
NN |
denotes |
E15 |
T6559 |
23176-23177 |
DT |
denotes |
a |
T6560 |
23178-23186 |
NN |
denotes |
staining |
T6561 |
23187-23190 |
IN |
denotes |
for |
T6562 |
23191-23198 |
NN |
denotes |
Annexin |
T6563 |
23199-23201 |
NN |
denotes |
A7 |
T6564 |
23202-23208 |
RB |
denotes |
mainly |
T6565 |
23209-23211 |
IN |
denotes |
in |
T6566 |
23212-23215 |
DT |
denotes |
the |
T6567 |
23216-23223 |
NN |
denotes |
cytosol |
T6568 |
23223-23224 |
. |
denotes |
. |
T6569 |
23224-23392 |
sentence |
denotes |
But this staining largely disappears and at the following day E16 we detect Annexin A7 in the nucleus of cells in the intermediate and marginal zone of the neopallium. |
T6570 |
23225-23228 |
CC |
denotes |
But |
T6571 |
23251-23261 |
VBZ |
denotes |
disappears |
T6572 |
23229-23233 |
DT |
denotes |
this |
T6573 |
23234-23242 |
NN |
denotes |
staining |
T6574 |
23243-23250 |
RB |
denotes |
largely |
T6575 |
23262-23265 |
CC |
denotes |
and |
T6576 |
23266-23268 |
IN |
denotes |
at |
T6577 |
23294-23300 |
VBP |
denotes |
detect |
T6578 |
23269-23272 |
DT |
denotes |
the |
T6579 |
23283-23286 |
NN |
denotes |
day |
T6580 |
23273-23282 |
VBG |
denotes |
following |
T6581 |
23287-23290 |
NN |
denotes |
E16 |
T6582 |
23291-23293 |
PRP |
denotes |
we |
T6583 |
23301-23308 |
NN |
denotes |
Annexin |
T6584 |
23309-23311 |
NN |
denotes |
A7 |
T6585 |
23312-23314 |
IN |
denotes |
in |
T6586 |
23315-23318 |
DT |
denotes |
the |
T6587 |
23319-23326 |
NN |
denotes |
nucleus |
T6588 |
23327-23329 |
IN |
denotes |
of |
T6589 |
23330-23335 |
NNS |
denotes |
cells |
T6590 |
23336-23338 |
IN |
denotes |
in |
T6591 |
23339-23342 |
DT |
denotes |
the |
T6592 |
23369-23373 |
NN |
denotes |
zone |
T6593 |
23343-23355 |
JJ |
denotes |
intermediate |
T6594 |
23356-23359 |
CC |
denotes |
and |
T6595 |
23360-23368 |
JJ |
denotes |
marginal |
T6596 |
23374-23376 |
IN |
denotes |
of |
T6597 |
23377-23380 |
DT |
denotes |
the |
T6598 |
23381-23391 |
NN |
denotes |
neopallium |
T6599 |
23391-23392 |
. |
denotes |
. |
T6600 |
23392-23702 |
sentence |
denotes |
The different expression patterns of Annexin A7-positive cells from the ventricular germinative zone to the marginal zone of the later neocortex observed in this study are similar to the developmental patterns of Tenascin-C-positive astroglial precursors following the guidance of the radial glial cells [18]. |
T6601 |
23393-23396 |
DT |
denotes |
The |
T6602 |
23418-23426 |
NNS |
denotes |
patterns |
T6603 |
23397-23406 |
JJ |
denotes |
different |
T6604 |
23407-23417 |
NN |
denotes |
expression |
T6605 |
23561-23564 |
VBP |
denotes |
are |
T6606 |
23427-23429 |
IN |
denotes |
of |
T6607 |
23430-23437 |
NN |
denotes |
Annexin |
T6608 |
23438-23440 |
NN |
denotes |
A7 |
T6609 |
23441-23449 |
JJ |
denotes |
positive |
T6610 |
23440-23441 |
HYPH |
denotes |
- |
T6611 |
23450-23455 |
NNS |
denotes |
cells |
T6612 |
23456-23460 |
IN |
denotes |
from |
T6613 |
23461-23464 |
DT |
denotes |
the |
T6614 |
23489-23493 |
NN |
denotes |
zone |
T6615 |
23465-23476 |
JJ |
denotes |
ventricular |
T6616 |
23477-23488 |
JJ |
denotes |
germinative |
T6617 |
23494-23496 |
IN |
denotes |
to |
T6618 |
23497-23500 |
DT |
denotes |
the |
T6619 |
23510-23514 |
NN |
denotes |
zone |
T6620 |
23501-23509 |
JJ |
denotes |
marginal |
T6621 |
23515-23517 |
IN |
denotes |
of |
T6622 |
23518-23521 |
DT |
denotes |
the |
T6623 |
23528-23537 |
NN |
denotes |
neocortex |
T6624 |
23522-23527 |
JJ |
denotes |
later |
T6625 |
23538-23546 |
VBN |
denotes |
observed |
T6626 |
23547-23549 |
IN |
denotes |
in |
T6627 |
23550-23554 |
DT |
denotes |
this |
T6628 |
23555-23560 |
NN |
denotes |
study |
T6629 |
23565-23572 |
JJ |
denotes |
similar |
T6630 |
23573-23575 |
IN |
denotes |
to |
T6631 |
23576-23579 |
DT |
denotes |
the |
T6632 |
23594-23602 |
NNS |
denotes |
patterns |
T6633 |
23580-23593 |
JJ |
denotes |
developmental |
T6634 |
23603-23605 |
IN |
denotes |
of |
T6635 |
23606-23614 |
NN |
denotes |
Tenascin |
T6636 |
23615-23616 |
NN |
denotes |
C |
T6637 |
23614-23615 |
HYPH |
denotes |
- |
T6638 |
23617-23625 |
JJ |
denotes |
positive |
T6639 |
23616-23617 |
HYPH |
denotes |
- |
T6640 |
23637-23647 |
NNS |
denotes |
precursors |
T6641 |
23626-23636 |
JJ |
denotes |
astroglial |
T6642 |
23648-23657 |
VBG |
denotes |
following |
T6643 |
23658-23661 |
DT |
denotes |
the |
T6644 |
23662-23670 |
NN |
denotes |
guidance |
T6645 |
23671-23673 |
IN |
denotes |
of |
T6646 |
23674-23677 |
DT |
denotes |
the |
T6647 |
23691-23696 |
NNS |
denotes |
cells |
T6648 |
23678-23684 |
JJ |
denotes |
radial |
T6649 |
23685-23690 |
JJ |
denotes |
glial |
T6650 |
23697-23698 |
-LRB- |
denotes |
[ |
T6651 |
23698-23700 |
CD |
denotes |
18 |
T6652 |
23700-23701 |
-RRB- |
denotes |
] |
T6653 |
23701-23702 |
. |
denotes |
. |
T6654 |
23702-23811 |
sentence |
denotes |
Moreover, the patterns resemble that of migrating and differentiated neurons described by Berry et al. [13]. |
T6655 |
23703-23711 |
RB |
denotes |
Moreover |
T6656 |
23726-23734 |
VBP |
denotes |
resemble |
T6657 |
23711-23713 |
, |
denotes |
, |
T6658 |
23713-23716 |
DT |
denotes |
the |
T6659 |
23717-23725 |
NNS |
denotes |
patterns |
T6660 |
23735-23739 |
DT |
denotes |
that |
T6661 |
23740-23742 |
IN |
denotes |
of |
T6662 |
23743-23752 |
VBG |
denotes |
migrating |
T6663 |
23772-23779 |
NNS |
denotes |
neurons |
T6664 |
23753-23756 |
CC |
denotes |
and |
T6665 |
23757-23771 |
VBN |
denotes |
differentiated |
T6666 |
23780-23789 |
VBN |
denotes |
described |
T6667 |
23790-23792 |
IN |
denotes |
by |
T6668 |
23793-23798 |
NNP |
denotes |
Berry |
T6669 |
23799-23801 |
FW |
denotes |
et |
T6670 |
23802-23805 |
FW |
denotes |
al. |
T6671 |
23806-23807 |
-LRB- |
denotes |
[ |
T6672 |
23807-23809 |
CD |
denotes |
13 |
T6673 |
23809-23810 |
-RRB- |
denotes |
] |
T6674 |
23810-23811 |
. |
denotes |
. |
T6675 |
23811-24062 |
sentence |
denotes |
Neurons that are generated prenatally in the proliferative ventricular layer of the neopallial cortex subsequently migrate through the intermediate zone to form the different cortical cell layers in a declining inside-out gradient of cell maturation. |
T6676 |
23812-23819 |
NNS |
denotes |
Neurons |
T6677 |
23927-23934 |
VBP |
denotes |
migrate |
T6678 |
23820-23824 |
WDT |
denotes |
that |
T6679 |
23829-23838 |
VBN |
denotes |
generated |
T6680 |
23825-23828 |
VBP |
denotes |
are |
T6681 |
23839-23849 |
RB |
denotes |
prenatally |
T6682 |
23850-23852 |
IN |
denotes |
in |
T6683 |
23853-23856 |
DT |
denotes |
the |
T6684 |
23883-23888 |
NN |
denotes |
layer |
T6685 |
23857-23870 |
JJ |
denotes |
proliferative |
T6686 |
23871-23882 |
JJ |
denotes |
ventricular |
T6687 |
23889-23891 |
IN |
denotes |
of |
T6688 |
23892-23895 |
DT |
denotes |
the |
T6689 |
23907-23913 |
NN |
denotes |
cortex |
T6690 |
23896-23906 |
JJ |
denotes |
neopallial |
T6691 |
23914-23926 |
RB |
denotes |
subsequently |
T6692 |
23935-23942 |
IN |
denotes |
through |
T6693 |
23943-23946 |
DT |
denotes |
the |
T6694 |
23960-23964 |
NN |
denotes |
zone |
T6695 |
23947-23959 |
JJ |
denotes |
intermediate |
T6696 |
23965-23967 |
TO |
denotes |
to |
T6697 |
23968-23972 |
VB |
denotes |
form |
T6698 |
23973-23976 |
DT |
denotes |
the |
T6699 |
24001-24007 |
NNS |
denotes |
layers |
T6700 |
23977-23986 |
JJ |
denotes |
different |
T6701 |
23987-23995 |
JJ |
denotes |
cortical |
T6702 |
23996-24000 |
NN |
denotes |
cell |
T6703 |
24008-24010 |
IN |
denotes |
in |
T6704 |
24011-24012 |
DT |
denotes |
a |
T6705 |
24034-24042 |
NN |
denotes |
gradient |
T6706 |
24013-24022 |
VBG |
denotes |
declining |
T6707 |
24023-24029 |
JJ |
denotes |
inside |
T6708 |
24030-24033 |
JJ |
denotes |
out |
T6709 |
24029-24030 |
HYPH |
denotes |
- |
T6710 |
24043-24045 |
IN |
denotes |
of |
T6711 |
24046-24050 |
NN |
denotes |
cell |
T6712 |
24051-24061 |
NN |
denotes |
maturation |
T6713 |
24061-24062 |
. |
denotes |
. |
T6714 |
24062-24185 |
sentence |
denotes |
These observations suggested that the subcellular localization of Annexin A7 depends on developmental stage and cell type. |
T6715 |
24063-24068 |
DT |
denotes |
These |
T6716 |
24069-24081 |
NNS |
denotes |
observations |
T6717 |
24082-24091 |
VBD |
denotes |
suggested |
T6718 |
24092-24096 |
IN |
denotes |
that |
T6719 |
24140-24147 |
VBZ |
denotes |
depends |
T6720 |
24097-24100 |
DT |
denotes |
the |
T6721 |
24113-24125 |
NN |
denotes |
localization |
T6722 |
24101-24112 |
JJ |
denotes |
subcellular |
T6723 |
24126-24128 |
IN |
denotes |
of |
T6724 |
24129-24136 |
NN |
denotes |
Annexin |
T6725 |
24137-24139 |
NN |
denotes |
A7 |
T6726 |
24148-24150 |
IN |
denotes |
on |
T6727 |
24151-24164 |
JJ |
denotes |
developmental |
T6728 |
24165-24170 |
NN |
denotes |
stage |
T6729 |
24171-24174 |
CC |
denotes |
and |
T6730 |
24175-24179 |
NN |
denotes |
cell |
T6731 |
24180-24184 |
NN |
denotes |
type |
T6732 |
24184-24185 |
. |
denotes |
. |
T6733 |
24185-24352 |
sentence |
denotes |
In the adult brain we generally observed a nuclear localization for Annexin A7 in neurons whereas astrocytes exhibited both a cytosolic as well as a nuclear staining. |
T6734 |
24186-24188 |
IN |
denotes |
In |
T6735 |
24218-24226 |
VBD |
denotes |
observed |
T6736 |
24189-24192 |
DT |
denotes |
the |
T6737 |
24199-24204 |
NN |
denotes |
brain |
T6738 |
24193-24198 |
JJ |
denotes |
adult |
T6739 |
24205-24207 |
PRP |
denotes |
we |
T6740 |
24208-24217 |
RB |
denotes |
generally |
T6741 |
24227-24228 |
DT |
denotes |
a |
T6742 |
24237-24249 |
NN |
denotes |
localization |
T6743 |
24229-24236 |
JJ |
denotes |
nuclear |
T6744 |
24250-24253 |
IN |
denotes |
for |
T6745 |
24254-24261 |
NN |
denotes |
Annexin |
T6746 |
24262-24264 |
NN |
denotes |
A7 |
T6747 |
24265-24267 |
IN |
denotes |
in |
T6748 |
24268-24275 |
NNS |
denotes |
neurons |
T6749 |
24276-24283 |
IN |
denotes |
whereas |
T6750 |
24295-24304 |
VBD |
denotes |
exhibited |
T6751 |
24284-24294 |
NNS |
denotes |
astrocytes |
T6752 |
24305-24309 |
CC |
denotes |
both |
T6753 |
24312-24321 |
JJ |
denotes |
cytosolic |
T6754 |
24310-24311 |
DT |
denotes |
a |
T6755 |
24322-24324 |
RB |
denotes |
as |
T6756 |
24330-24332 |
IN |
denotes |
as |
T6757 |
24325-24329 |
RB |
denotes |
well |
T6758 |
24333-24334 |
DT |
denotes |
a |
T6759 |
24335-24342 |
JJ |
denotes |
nuclear |
T6760 |
24343-24351 |
NN |
denotes |
staining |
T6761 |
24351-24352 |
. |
denotes |
. |
T6762 |
24352-24527 |
sentence |
denotes |
However, pyramidal neurons of the isocortex and Purkinje-cells of the cerebellum exhibited a cytosolic stain of intermediate intensity including their neurites and dendrites. |
T6763 |
24353-24360 |
RB |
denotes |
However |
T6764 |
24434-24443 |
VBD |
denotes |
exhibited |
T6765 |
24360-24362 |
, |
denotes |
, |
T6766 |
24362-24371 |
JJ |
denotes |
pyramidal |
T6767 |
24372-24379 |
NNS |
denotes |
neurons |
T6768 |
24380-24382 |
IN |
denotes |
of |
T6769 |
24383-24386 |
DT |
denotes |
the |
T6770 |
24387-24396 |
NN |
denotes |
isocortex |
T6771 |
24397-24400 |
CC |
denotes |
and |
T6772 |
24401-24409 |
NNP |
denotes |
Purkinje |
T6773 |
24410-24415 |
NNS |
denotes |
cells |
T6774 |
24409-24410 |
HYPH |
denotes |
- |
T6775 |
24416-24418 |
IN |
denotes |
of |
T6776 |
24419-24422 |
DT |
denotes |
the |
T6777 |
24423-24433 |
NN |
denotes |
cerebellum |
T6778 |
24444-24445 |
DT |
denotes |
a |
T6779 |
24456-24461 |
NN |
denotes |
stain |
T6780 |
24446-24455 |
JJ |
denotes |
cytosolic |
T6781 |
24462-24464 |
IN |
denotes |
of |
T6782 |
24465-24477 |
JJ |
denotes |
intermediate |
T6783 |
24478-24487 |
NN |
denotes |
intensity |
T6784 |
24488-24497 |
VBG |
denotes |
including |
T6785 |
24498-24503 |
PRP$ |
denotes |
their |
T6786 |
24504-24512 |
NNS |
denotes |
neurites |
T6787 |
24513-24516 |
CC |
denotes |
and |
T6788 |
24517-24526 |
NNS |
denotes |
dendrites |
T6789 |
24526-24527 |
. |
denotes |
. |
T6790 |
24527-24819 |
sentence |
denotes |
We have previously reported the expression of Annexin A7 in human temporal neocortex and hippocampus obtained from neurosurgery for therapy-refractory epilepsy and found the two Annexin A7 isoforms restricted to the cytoplasm and nuclei of astrocytes, whereas neurons lacked any signal [19]. |
T6791 |
24528-24530 |
PRP |
denotes |
We |
T6792 |
24547-24555 |
VBN |
denotes |
reported |
T6793 |
24531-24535 |
VBP |
denotes |
have |
T6794 |
24536-24546 |
RB |
denotes |
previously |
T6795 |
24556-24559 |
DT |
denotes |
the |
T6796 |
24560-24570 |
NN |
denotes |
expression |
T6797 |
24571-24573 |
IN |
denotes |
of |
T6798 |
24574-24581 |
NN |
denotes |
Annexin |
T6799 |
24582-24584 |
NN |
denotes |
A7 |
T6800 |
24585-24587 |
IN |
denotes |
in |
T6801 |
24588-24593 |
JJ |
denotes |
human |
T6802 |
24603-24612 |
NN |
denotes |
neocortex |
T6803 |
24594-24602 |
JJ |
denotes |
temporal |
T6804 |
24613-24616 |
CC |
denotes |
and |
T6805 |
24617-24628 |
NN |
denotes |
hippocampus |
T6806 |
24629-24637 |
VBN |
denotes |
obtained |
T6807 |
24638-24642 |
IN |
denotes |
from |
T6808 |
24643-24655 |
NN |
denotes |
neurosurgery |
T6809 |
24656-24659 |
IN |
denotes |
for |
T6810 |
24660-24667 |
NN |
denotes |
therapy |
T6811 |
24668-24678 |
JJ |
denotes |
refractory |
T6812 |
24667-24668 |
HYPH |
denotes |
- |
T6813 |
24679-24687 |
NN |
denotes |
epilepsy |
T6814 |
24688-24691 |
CC |
denotes |
and |
T6815 |
24692-24697 |
VBD |
denotes |
found |
T6816 |
24698-24701 |
DT |
denotes |
the |
T6817 |
24717-24725 |
NNS |
denotes |
isoforms |
T6818 |
24702-24705 |
CD |
denotes |
two |
T6819 |
24706-24713 |
NN |
denotes |
Annexin |
T6820 |
24714-24716 |
NN |
denotes |
A7 |
T6821 |
24726-24736 |
VBN |
denotes |
restricted |
T6822 |
24737-24739 |
IN |
denotes |
to |
T6823 |
24740-24743 |
DT |
denotes |
the |
T6824 |
24744-24753 |
NN |
denotes |
cytoplasm |
T6825 |
24754-24757 |
CC |
denotes |
and |
T6826 |
24758-24764 |
NNS |
denotes |
nuclei |
T6827 |
24765-24767 |
IN |
denotes |
of |
T6828 |
24768-24778 |
NNS |
denotes |
astrocytes |
T6829 |
24778-24780 |
, |
denotes |
, |
T6830 |
24780-24787 |
IN |
denotes |
whereas |
T6831 |
24796-24802 |
VBD |
denotes |
lacked |
T6832 |
24788-24795 |
NNS |
denotes |
neurons |
T6833 |
24803-24806 |
DT |
denotes |
any |
T6834 |
24807-24813 |
NN |
denotes |
signal |
T6835 |
24814-24815 |
-LRB- |
denotes |
[ |
T6836 |
24815-24817 |
CD |
denotes |
19 |
T6837 |
24817-24818 |
-RRB- |
denotes |
] |
T6838 |
24818-24819 |
. |
denotes |
. |
T6839 |
24819-24979 |
sentence |
denotes |
The hippocampal area showed typical signs of Ammon's horn sclerosis, but the adjacent temporal neocortex tissue did not show any histopathological alterations. |
T6840 |
24820-24823 |
DT |
denotes |
The |
T6841 |
24836-24840 |
NN |
denotes |
area |
T6842 |
24824-24835 |
JJ |
denotes |
hippocampal |
T6843 |
24841-24847 |
VBD |
denotes |
showed |
T6844 |
24848-24855 |
JJ |
denotes |
typical |
T6845 |
24856-24861 |
NNS |
denotes |
signs |
T6846 |
24862-24864 |
IN |
denotes |
of |
T6847 |
24865-24870 |
NNP |
denotes |
Ammon |
T6848 |
24878-24887 |
NN |
denotes |
sclerosis |
T6849 |
24870-24872 |
POS |
denotes |
's |
T6850 |
24873-24877 |
NN |
denotes |
horn |
T6851 |
24887-24889 |
, |
denotes |
, |
T6852 |
24889-24892 |
CC |
denotes |
but |
T6853 |
24893-24896 |
DT |
denotes |
the |
T6854 |
24925-24931 |
NN |
denotes |
tissue |
T6855 |
24897-24905 |
JJ |
denotes |
adjacent |
T6856 |
24906-24914 |
JJ |
denotes |
temporal |
T6857 |
24915-24924 |
NN |
denotes |
neocortex |
T6858 |
24940-24944 |
VB |
denotes |
show |
T6859 |
24932-24935 |
VBD |
denotes |
did |
T6860 |
24936-24939 |
RB |
denotes |
not |
T6861 |
24945-24948 |
DT |
denotes |
any |
T6862 |
24967-24978 |
NNS |
denotes |
alterations |
T6863 |
24949-24966 |
JJ |
denotes |
histopathological |
T6864 |
24978-24979 |
. |
denotes |
. |
T6865 |
24979-25056 |
sentence |
denotes |
This data is in discrepancy with our actual observation in the murine brain. |
T6866 |
24980-24984 |
DT |
denotes |
This |
T6867 |
24985-24989 |
NNS |
denotes |
data |
T6868 |
24990-24992 |
VBZ |
denotes |
is |
T6869 |
24993-24995 |
IN |
denotes |
in |
T6870 |
24996-25007 |
NN |
denotes |
discrepancy |
T6871 |
25008-25012 |
IN |
denotes |
with |
T6872 |
25013-25016 |
PRP$ |
denotes |
our |
T6873 |
25024-25035 |
NN |
denotes |
observation |
T6874 |
25017-25023 |
JJ |
denotes |
actual |
T6875 |
25036-25038 |
IN |
denotes |
in |
T6876 |
25039-25042 |
DT |
denotes |
the |
T6877 |
25050-25055 |
NN |
denotes |
brain |
T6878 |
25043-25049 |
JJ |
denotes |
murine |
T6879 |
25055-25056 |
. |
denotes |
. |
T6880 |
25056-25247 |
sentence |
denotes |
To determine if this is due to different methods in immunofluorescence staining or indeed different expression patterns in mouse and human, we repeated the immunofluorescence of human brain. |
T6881 |
25057-25059 |
TO |
denotes |
To |
T6882 |
25060-25069 |
VB |
denotes |
determine |
T6883 |
25200-25208 |
VBD |
denotes |
repeated |
T6884 |
25070-25072 |
IN |
denotes |
if |
T6885 |
25078-25080 |
VBZ |
denotes |
is |
T6886 |
25073-25077 |
DT |
denotes |
this |
T6887 |
25081-25084 |
IN |
denotes |
due |
T6888 |
25085-25087 |
IN |
denotes |
to |
T6889 |
25088-25097 |
JJ |
denotes |
different |
T6890 |
25098-25105 |
NNS |
denotes |
methods |
T6891 |
25106-25108 |
IN |
denotes |
in |
T6892 |
25109-25127 |
NN |
denotes |
immunofluorescence |
T6893 |
25128-25136 |
NN |
denotes |
staining |
T6894 |
25137-25139 |
CC |
denotes |
or |
T6895 |
25140-25146 |
RB |
denotes |
indeed |
T6896 |
25168-25176 |
NNS |
denotes |
patterns |
T6897 |
25147-25156 |
JJ |
denotes |
different |
T6898 |
25157-25167 |
NN |
denotes |
expression |
T6899 |
25177-25179 |
IN |
denotes |
in |
T6900 |
25180-25185 |
NN |
denotes |
mouse |
T6901 |
25186-25189 |
CC |
denotes |
and |
T6902 |
25190-25195 |
JJ |
denotes |
human |
T6903 |
25195-25197 |
, |
denotes |
, |
T6904 |
25197-25199 |
PRP |
denotes |
we |
T6905 |
25209-25212 |
DT |
denotes |
the |
T6906 |
25213-25231 |
NN |
denotes |
immunofluorescence |
T6907 |
25232-25234 |
IN |
denotes |
of |
T6908 |
25235-25240 |
JJ |
denotes |
human |
T6909 |
25241-25246 |
NN |
denotes |
brain |
T6910 |
25246-25247 |
. |
denotes |
. |
T6911 |
25247-25327 |
sentence |
denotes |
This time we investigated sections from human parietal cortex of autopsy brain. |
T6912 |
25248-25252 |
DT |
denotes |
This |
T6913 |
25253-25257 |
NN |
denotes |
time |
T6914 |
25261-25273 |
VBD |
denotes |
investigated |
T6915 |
25258-25260 |
PRP |
denotes |
we |
T6916 |
25274-25282 |
NNS |
denotes |
sections |
T6917 |
25283-25287 |
IN |
denotes |
from |
T6918 |
25288-25293 |
JJ |
denotes |
human |
T6919 |
25303-25309 |
NN |
denotes |
cortex |
T6920 |
25294-25302 |
JJ |
denotes |
parietal |
T6921 |
25310-25312 |
IN |
denotes |
of |
T6922 |
25313-25320 |
NN |
denotes |
autopsy |
T6923 |
25321-25326 |
NN |
denotes |
brain |
T6924 |
25326-25327 |
. |
denotes |
. |
T6925 |
25327-25447 |
sentence |
denotes |
The astroglial expression of Annexin A7 could be confirmed, although not all astrocytes exhibited the nuclear staining. |
T6926 |
25328-25331 |
DT |
denotes |
The |
T6927 |
25343-25353 |
NN |
denotes |
expression |
T6928 |
25332-25342 |
JJ |
denotes |
astroglial |
T6929 |
25377-25386 |
VBN |
denotes |
confirmed |
T6930 |
25354-25356 |
IN |
denotes |
of |
T6931 |
25357-25364 |
NN |
denotes |
Annexin |
T6932 |
25365-25367 |
NN |
denotes |
A7 |
T6933 |
25368-25373 |
MD |
denotes |
could |
T6934 |
25374-25376 |
VB |
denotes |
be |
T6935 |
25386-25388 |
, |
denotes |
, |
T6936 |
25388-25396 |
IN |
denotes |
although |
T6937 |
25416-25425 |
VBD |
denotes |
exhibited |
T6938 |
25397-25400 |
RB |
denotes |
not |
T6939 |
25405-25415 |
NNS |
denotes |
astrocytes |
T6940 |
25401-25404 |
DT |
denotes |
all |
T6941 |
25426-25429 |
DT |
denotes |
the |
T6942 |
25438-25446 |
NN |
denotes |
staining |
T6943 |
25430-25437 |
JJ |
denotes |
nuclear |
T6944 |
25446-25447 |
. |
denotes |
. |
T6945 |
25447-25555 |
sentence |
denotes |
Pyramidal neurons however indicated a distinct staining of Annexin A7 most prominent along their dendrites. |
T6946 |
25448-25457 |
JJ |
denotes |
Pyramidal |
T6947 |
25458-25465 |
NNS |
denotes |
neurons |
T6948 |
25474-25483 |
VBD |
denotes |
indicated |
T6949 |
25466-25473 |
RB |
denotes |
however |
T6950 |
25484-25485 |
DT |
denotes |
a |
T6951 |
25495-25503 |
NN |
denotes |
staining |
T6952 |
25486-25494 |
JJ |
denotes |
distinct |
T6953 |
25504-25506 |
IN |
denotes |
of |
T6954 |
25507-25514 |
NN |
denotes |
Annexin |
T6955 |
25515-25517 |
NN |
denotes |
A7 |
T6956 |
25518-25522 |
RBS |
denotes |
most |
T6957 |
25523-25532 |
JJ |
denotes |
prominent |
T6958 |
25533-25538 |
IN |
denotes |
along |
T6959 |
25539-25544 |
PRP$ |
denotes |
their |
T6960 |
25545-25554 |
NNS |
denotes |
dendrites |
T6961 |
25554-25555 |
. |
denotes |
. |
T6962 |
25555-25758 |
sentence |
denotes |
The different results obtained for the neurons may be explained by the fact that we previously used frozen tissue sections or that they were derived from patients suffering from temporal lobe epilepsia. |
T6963 |
25556-25559 |
DT |
denotes |
The |
T6964 |
25570-25577 |
NNS |
denotes |
results |
T6965 |
25560-25569 |
JJ |
denotes |
different |
T6966 |
25610-25619 |
VBN |
denotes |
explained |
T6967 |
25578-25586 |
VBN |
denotes |
obtained |
T6968 |
25587-25590 |
IN |
denotes |
for |
T6969 |
25591-25594 |
DT |
denotes |
the |
T6970 |
25595-25602 |
NNS |
denotes |
neurons |
T6971 |
25603-25606 |
MD |
denotes |
may |
T6972 |
25607-25609 |
VB |
denotes |
be |
T6973 |
25620-25622 |
IN |
denotes |
by |
T6974 |
25623-25626 |
DT |
denotes |
the |
T6975 |
25627-25631 |
NN |
denotes |
fact |
T6976 |
25632-25636 |
IN |
denotes |
that |
T6977 |
25651-25655 |
VBD |
denotes |
used |
T6978 |
25637-25639 |
PRP |
denotes |
we |
T6979 |
25640-25650 |
RB |
denotes |
previously |
T6980 |
25656-25662 |
JJ |
denotes |
frozen |
T6981 |
25670-25678 |
NNS |
denotes |
sections |
T6982 |
25663-25669 |
NN |
denotes |
tissue |
T6983 |
25679-25681 |
CC |
denotes |
or |
T6984 |
25682-25686 |
IN |
denotes |
that |
T6985 |
25697-25704 |
VBN |
denotes |
derived |
T6986 |
25687-25691 |
PRP |
denotes |
they |
T6987 |
25692-25696 |
VBD |
denotes |
were |
T6988 |
25705-25709 |
IN |
denotes |
from |
T6989 |
25710-25718 |
NNS |
denotes |
patients |
T6990 |
25719-25728 |
VBG |
denotes |
suffering |
T6991 |
25729-25733 |
IN |
denotes |
from |
T6992 |
25734-25742 |
JJ |
denotes |
temporal |
T6993 |
25743-25747 |
NN |
denotes |
lobe |
T6994 |
25748-25757 |
NN |
denotes |
epilepsia |
T6995 |
25757-25758 |
. |
denotes |
. |
T6996 |
25758-25904 |
sentence |
denotes |
On the other hand the actual human brain tissue used was from aged patients and did not correspond to the age of the mice included in this study. |
T6997 |
25759-25761 |
IN |
denotes |
On |
T6998 |
25812-25815 |
VBD |
denotes |
was |
T6999 |
25762-25765 |
DT |
denotes |
the |
T7000 |
25772-25776 |
NN |
denotes |
hand |
T7001 |
25766-25771 |
JJ |
denotes |
other |
T7002 |
25777-25780 |
DT |
denotes |
the |
T7003 |
25800-25806 |
NN |
denotes |
tissue |
T7004 |
25781-25787 |
JJ |
denotes |
actual |
T7005 |
25788-25793 |
JJ |
denotes |
human |
T7006 |
25794-25799 |
NN |
denotes |
brain |
T7007 |
25807-25811 |
VBN |
denotes |
used |
T7008 |
25816-25820 |
IN |
denotes |
from |
T7009 |
25821-25825 |
JJ |
denotes |
aged |
T7010 |
25826-25834 |
NNS |
denotes |
patients |
T7011 |
25835-25838 |
CC |
denotes |
and |
T7012 |
25839-25842 |
VBD |
denotes |
did |
T7013 |
25847-25857 |
VB |
denotes |
correspond |
T7014 |
25843-25846 |
RB |
denotes |
not |
T7015 |
25858-25860 |
IN |
denotes |
to |
T7016 |
25861-25864 |
DT |
denotes |
the |
T7017 |
25865-25868 |
NN |
denotes |
age |
T7018 |
25869-25871 |
IN |
denotes |
of |
T7019 |
25872-25875 |
DT |
denotes |
the |
T7020 |
25876-25880 |
NNS |
denotes |
mice |
T7021 |
25881-25889 |
VBN |
denotes |
included |
T7022 |
25890-25892 |
IN |
denotes |
in |
T7023 |
25893-25897 |
DT |
denotes |
this |
T7024 |
25898-25903 |
NN |
denotes |
study |
T7025 |
25903-25904 |
. |
denotes |
. |
T7026 |
25904-26093 |
sentence |
denotes |
In cultured cells after cell damage or apoptosis (unpublished observations) or in cells treated with a Ca2+-ionophore Annexin A7 translocated from the cytoplasm to cellular membranes [19]. |
T7027 |
25905-25907 |
IN |
denotes |
In |
T7028 |
26034-26046 |
VBN |
denotes |
translocated |
T7029 |
25908-25916 |
VBN |
denotes |
cultured |
T7030 |
25917-25922 |
NNS |
denotes |
cells |
T7031 |
25923-25928 |
IN |
denotes |
after |
T7032 |
25929-25933 |
NN |
denotes |
cell |
T7033 |
25934-25940 |
NN |
denotes |
damage |
T7034 |
25941-25943 |
CC |
denotes |
or |
T7035 |
25944-25953 |
NN |
denotes |
apoptosis |
T7036 |
25954-25955 |
-LRB- |
denotes |
( |
T7037 |
25967-25979 |
NNS |
denotes |
observations |
T7038 |
25955-25966 |
JJ |
denotes |
unpublished |
T7039 |
25979-25980 |
-RRB- |
denotes |
) |
T7040 |
25981-25983 |
CC |
denotes |
or |
T7041 |
25984-25986 |
IN |
denotes |
in |
T7042 |
25987-25992 |
NNS |
denotes |
cells |
T7043 |
25993-26000 |
VBN |
denotes |
treated |
T7044 |
26001-26005 |
IN |
denotes |
with |
T7045 |
26006-26007 |
DT |
denotes |
a |
T7046 |
26013-26022 |
NN |
denotes |
ionophore |
T7047 |
26008-26012 |
NN |
denotes |
Ca2+ |
T7048 |
26012-26013 |
HYPH |
denotes |
- |
T7049 |
26023-26030 |
NN |
denotes |
Annexin |
T7050 |
26031-26033 |
NN |
denotes |
A7 |
T7051 |
26047-26051 |
IN |
denotes |
from |
T7052 |
26052-26055 |
DT |
denotes |
the |
T7053 |
26056-26065 |
NN |
denotes |
cytoplasm |
T7054 |
26066-26068 |
IN |
denotes |
to |
T7055 |
26069-26077 |
JJ |
denotes |
cellular |
T7056 |
26078-26087 |
NNS |
denotes |
membranes |
T7057 |
26088-26089 |
-LRB- |
denotes |
[ |
T7058 |
26089-26091 |
CD |
denotes |
19 |
T7059 |
26091-26092 |
-RRB- |
denotes |
] |
T7060 |
26092-26093 |
. |
denotes |
. |
T7061 |
26093-26253 |
sentence |
denotes |
We therefore favor the hypothesis that Annexin A7 in the sensitive neurons of the human autopsy brain may have similarly translocated to the cellular membrane. |
T7062 |
26094-26096 |
PRP |
denotes |
We |
T7063 |
26107-26112 |
VBP |
denotes |
favor |
T7064 |
26097-26106 |
RB |
denotes |
therefore |
T7065 |
26113-26116 |
DT |
denotes |
the |
T7066 |
26117-26127 |
NN |
denotes |
hypothesis |
T7067 |
26128-26132 |
IN |
denotes |
that |
T7068 |
26215-26227 |
VBN |
denotes |
translocated |
T7069 |
26133-26140 |
NN |
denotes |
Annexin |
T7070 |
26141-26143 |
NN |
denotes |
A7 |
T7071 |
26144-26146 |
IN |
denotes |
in |
T7072 |
26147-26150 |
DT |
denotes |
the |
T7073 |
26161-26168 |
NNS |
denotes |
neurons |
T7074 |
26151-26160 |
JJ |
denotes |
sensitive |
T7075 |
26169-26171 |
IN |
denotes |
of |
T7076 |
26172-26175 |
DT |
denotes |
the |
T7077 |
26190-26195 |
NN |
denotes |
brain |
T7078 |
26176-26181 |
JJ |
denotes |
human |
T7079 |
26182-26189 |
NN |
denotes |
autopsy |
T7080 |
26196-26199 |
MD |
denotes |
may |
T7081 |
26200-26204 |
VB |
denotes |
have |
T7082 |
26205-26214 |
RB |
denotes |
similarly |
T7083 |
26228-26230 |
IN |
denotes |
to |
T7084 |
26231-26234 |
DT |
denotes |
the |
T7085 |
26244-26252 |
NN |
denotes |
membrane |
T7086 |
26235-26243 |
JJ |
denotes |
cellular |
T7087 |
26252-26253 |
. |
denotes |
. |
T7088 |
26253-26417 |
sentence |
denotes |
This property of translocation and membrane binding is common to all annexins and commercially available kits for apoptosis detection employ recombinant AnnexinA5. |
T7089 |
26254-26258 |
DT |
denotes |
This |
T7090 |
26259-26267 |
NN |
denotes |
property |
T7091 |
26306-26308 |
VBZ |
denotes |
is |
T7092 |
26268-26270 |
IN |
denotes |
of |
T7093 |
26271-26284 |
NN |
denotes |
translocation |
T7094 |
26285-26288 |
CC |
denotes |
and |
T7095 |
26289-26297 |
NN |
denotes |
membrane |
T7096 |
26298-26305 |
NN |
denotes |
binding |
T7097 |
26309-26315 |
JJ |
denotes |
common |
T7098 |
26316-26318 |
IN |
denotes |
to |
T7099 |
26319-26322 |
DT |
denotes |
all |
T7100 |
26323-26331 |
NNS |
denotes |
annexins |
T7101 |
26332-26335 |
CC |
denotes |
and |
T7102 |
26336-26348 |
RB |
denotes |
commercially |
T7103 |
26349-26358 |
JJ |
denotes |
available |
T7104 |
26359-26363 |
NNS |
denotes |
kits |
T7105 |
26388-26394 |
VBP |
denotes |
employ |
T7106 |
26364-26367 |
IN |
denotes |
for |
T7107 |
26368-26377 |
NN |
denotes |
apoptosis |
T7108 |
26378-26387 |
NN |
denotes |
detection |
T7109 |
26395-26406 |
JJ |
denotes |
recombinant |
T7110 |
26407-26416 |
NN |
denotes |
AnnexinA5 |
T7111 |
26416-26417 |
. |
denotes |
. |
T7112 |
26417-26598 |
sentence |
denotes |
The presence of nuclear Annexin A7 in murine brain was confirmed by controls using sections from the AnxA7-/- mouse and by a biochemical extraction of the protein from the nucleus. |
T7113 |
26418-26421 |
DT |
denotes |
The |
T7114 |
26422-26430 |
NN |
denotes |
presence |
T7115 |
26473-26482 |
VBN |
denotes |
confirmed |
T7116 |
26431-26433 |
IN |
denotes |
of |
T7117 |
26434-26441 |
JJ |
denotes |
nuclear |
T7118 |
26450-26452 |
NN |
denotes |
A7 |
T7119 |
26442-26449 |
NN |
denotes |
Annexin |
T7120 |
26453-26455 |
IN |
denotes |
in |
T7121 |
26456-26462 |
JJ |
denotes |
murine |
T7122 |
26463-26468 |
NN |
denotes |
brain |
T7123 |
26469-26472 |
VBD |
denotes |
was |
T7124 |
26483-26485 |
IN |
denotes |
by |
T7125 |
26486-26494 |
NNS |
denotes |
controls |
T7126 |
26495-26500 |
VBG |
denotes |
using |
T7127 |
26501-26509 |
NNS |
denotes |
sections |
T7128 |
26510-26514 |
IN |
denotes |
from |
T7129 |
26515-26518 |
DT |
denotes |
the |
T7130 |
26528-26533 |
NN |
denotes |
mouse |
T7131 |
26519-26524 |
NN |
denotes |
AnxA7 |
T7132 |
26524-26525 |
SYM |
denotes |
- |
T7133 |
26525-26526 |
HYPH |
denotes |
/ |
T7134 |
26526-26527 |
SYM |
denotes |
- |
T7135 |
26534-26537 |
CC |
denotes |
and |
T7136 |
26538-26540 |
IN |
denotes |
by |
T7137 |
26541-26542 |
DT |
denotes |
a |
T7138 |
26555-26565 |
NN |
denotes |
extraction |
T7139 |
26543-26554 |
JJ |
denotes |
biochemical |
T7140 |
26566-26568 |
IN |
denotes |
of |
T7141 |
26569-26572 |
DT |
denotes |
the |
T7142 |
26573-26580 |
NN |
denotes |
protein |
T7143 |
26581-26585 |
IN |
denotes |
from |
T7144 |
26586-26589 |
DT |
denotes |
the |
T7145 |
26590-26597 |
NN |
denotes |
nucleus |
T7146 |
26597-26598 |
. |
denotes |
. |
T7147 |
26598-26784 |
sentence |
denotes |
Both Annexin A7 isoforms described in brain tissue seemingly are expressed by neurons and astrocytes, which was shown using total cell extracts of cultured neuo-2a, PC-12, and C6 cells. |
T7148 |
26599-26603 |
DT |
denotes |
Both |
T7149 |
26615-26623 |
NNS |
denotes |
isoforms |
T7150 |
26604-26611 |
NN |
denotes |
Annexin |
T7151 |
26612-26614 |
NN |
denotes |
A7 |
T7152 |
26664-26673 |
VBN |
denotes |
expressed |
T7153 |
26624-26633 |
VBN |
denotes |
described |
T7154 |
26634-26636 |
IN |
denotes |
in |
T7155 |
26637-26642 |
NN |
denotes |
brain |
T7156 |
26643-26649 |
NN |
denotes |
tissue |
T7157 |
26650-26659 |
RB |
denotes |
seemingly |
T7158 |
26660-26663 |
VBP |
denotes |
are |
T7159 |
26674-26676 |
IN |
denotes |
by |
T7160 |
26677-26684 |
NNS |
denotes |
neurons |
T7161 |
26685-26688 |
CC |
denotes |
and |
T7162 |
26689-26699 |
NNS |
denotes |
astrocytes |
T7163 |
26699-26701 |
, |
denotes |
, |
T7164 |
26701-26706 |
WDT |
denotes |
which |
T7165 |
26711-26716 |
VBN |
denotes |
shown |
T7166 |
26707-26710 |
VBD |
denotes |
was |
T7167 |
26717-26722 |
VBG |
denotes |
using |
T7168 |
26723-26728 |
JJ |
denotes |
total |
T7169 |
26734-26742 |
NNS |
denotes |
extracts |
T7170 |
26729-26733 |
NN |
denotes |
cell |
T7171 |
26743-26745 |
IN |
denotes |
of |
T7172 |
26746-26754 |
VBN |
denotes |
cultured |
T7173 |
26778-26783 |
NNS |
denotes |
cells |
T7174 |
26755-26759 |
NN |
denotes |
neuo |
T7175 |
26759-26760 |
HYPH |
denotes |
- |
T7176 |
26760-26762 |
CD |
denotes |
2a |
T7177 |
26762-26764 |
, |
denotes |
, |
T7178 |
26764-26766 |
NN |
denotes |
PC |
T7179 |
26766-26767 |
HYPH |
denotes |
- |
T7180 |
26767-26769 |
CD |
denotes |
12 |
T7181 |
26769-26771 |
, |
denotes |
, |
T7182 |
26771-26774 |
CC |
denotes |
and |
T7183 |
26775-26777 |
NN |
denotes |
C6 |
T7184 |
26783-26784 |
. |
denotes |
. |
T7185 |
26784-26919 |
sentence |
denotes |
In addition to their expression in brain, both Annexin A7 isoforms have only been described in heart muscle and red blood cells [5-8]. |
T7186 |
26785-26787 |
IN |
denotes |
In |
T7187 |
26867-26876 |
VBN |
denotes |
described |
T7188 |
26788-26796 |
NN |
denotes |
addition |
T7189 |
26797-26799 |
IN |
denotes |
to |
T7190 |
26800-26805 |
PRP$ |
denotes |
their |
T7191 |
26806-26816 |
NN |
denotes |
expression |
T7192 |
26817-26819 |
IN |
denotes |
in |
T7193 |
26820-26825 |
NN |
denotes |
brain |
T7194 |
26825-26827 |
, |
denotes |
, |
T7195 |
26827-26831 |
CC |
denotes |
both |
T7196 |
26843-26851 |
NNS |
denotes |
isoforms |
T7197 |
26832-26839 |
NN |
denotes |
Annexin |
T7198 |
26840-26842 |
NN |
denotes |
A7 |
T7199 |
26852-26856 |
VBP |
denotes |
have |
T7200 |
26857-26861 |
RB |
denotes |
only |
T7201 |
26862-26866 |
VBN |
denotes |
been |
T7202 |
26877-26879 |
IN |
denotes |
in |
T7203 |
26880-26885 |
NN |
denotes |
heart |
T7204 |
26886-26892 |
NN |
denotes |
muscle |
T7205 |
26893-26896 |
CC |
denotes |
and |
T7206 |
26897-26900 |
JJ |
denotes |
red |
T7207 |
26907-26912 |
NNS |
denotes |
cells |
T7208 |
26901-26906 |
NN |
denotes |
blood |
T7209 |
26913-26914 |
-LRB- |
denotes |
[ |
T7210 |
26914-26915 |
CD |
denotes |
5 |
T7211 |
26915-26916 |
SYM |
denotes |
- |
T7212 |
26916-26917 |
CD |
denotes |
8 |
T7213 |
26917-26918 |
-RRB- |
denotes |
] |
T7214 |
26918-26919 |
. |
denotes |
. |
T7215 |
26919-27137 |
sentence |
denotes |
Although the inactivation of the annexin A7 gene did not interfere with the viability and development of knock out mice [10], their generation allowed us to address the role of Annexin A7 in specific cell types [8,9]. |
T7216 |
26920-26928 |
IN |
denotes |
Although |
T7217 |
26977-26986 |
VB |
denotes |
interfere |
T7218 |
26929-26932 |
DT |
denotes |
the |
T7219 |
26933-26945 |
NN |
denotes |
inactivation |
T7220 |
26946-26948 |
IN |
denotes |
of |
T7221 |
26949-26952 |
DT |
denotes |
the |
T7222 |
26964-26968 |
NN |
denotes |
gene |
T7223 |
26953-26960 |
NN |
denotes |
annexin |
T7224 |
26961-26963 |
NN |
denotes |
A7 |
T7225 |
26969-26972 |
VBD |
denotes |
did |
T7226 |
26973-26976 |
RB |
denotes |
not |
T7227 |
27063-27070 |
VBD |
denotes |
allowed |
T7228 |
26987-26991 |
IN |
denotes |
with |
T7229 |
26992-26995 |
DT |
denotes |
the |
T7230 |
26996-27005 |
NN |
denotes |
viability |
T7231 |
27006-27009 |
CC |
denotes |
and |
T7232 |
27010-27021 |
NN |
denotes |
development |
T7233 |
27022-27024 |
IN |
denotes |
of |
T7234 |
27025-27030 |
VB |
denotes |
knock |
T7235 |
27035-27039 |
NNS |
denotes |
mice |
T7236 |
27031-27034 |
RP |
denotes |
out |
T7237 |
27040-27041 |
-LRB- |
denotes |
[ |
T7238 |
27041-27043 |
CD |
denotes |
10 |
T7239 |
27043-27044 |
-RRB- |
denotes |
] |
T7240 |
27044-27046 |
, |
denotes |
, |
T7241 |
27046-27051 |
PRP$ |
denotes |
their |
T7242 |
27052-27062 |
NN |
denotes |
generation |
T7243 |
27071-27073 |
PRP |
denotes |
us |
T7244 |
27077-27084 |
VB |
denotes |
address |
T7245 |
27074-27076 |
TO |
denotes |
to |
T7246 |
27085-27088 |
DT |
denotes |
the |
T7247 |
27089-27093 |
NN |
denotes |
role |
T7248 |
27094-27096 |
IN |
denotes |
of |
T7249 |
27097-27104 |
NN |
denotes |
Annexin |
T7250 |
27105-27107 |
NN |
denotes |
A7 |
T7251 |
27108-27110 |
IN |
denotes |
in |
T7252 |
27111-27119 |
JJ |
denotes |
specific |
T7253 |
27125-27130 |
NNS |
denotes |
types |
T7254 |
27120-27124 |
NN |
denotes |
cell |
T7255 |
27131-27132 |
-LRB- |
denotes |
[ |
T7256 |
27134-27135 |
CD |
denotes |
9 |
T7257 |
27132-27133 |
CD |
denotes |
8 |
T7258 |
27133-27134 |
, |
denotes |
, |
T7259 |
27135-27136 |
-RRB- |
denotes |
] |
T7260 |
27136-27137 |
. |
denotes |
. |
T7261 |
27137-27384 |
sentence |
denotes |
Indeed, when we analyzed primary astrocytes from an AnxA7-/- mouse for Ca2+-dependent signaling processes, we found that they exhibited a significantly increased velocity of mechanically induced astrocytic Ca2+-waves as compared to wild type [9]. |
T7262 |
27138-27144 |
RB |
denotes |
Indeed |
T7263 |
27248-27253 |
VBD |
denotes |
found |
T7264 |
27144-27146 |
, |
denotes |
, |
T7265 |
27146-27150 |
WRB |
denotes |
when |
T7266 |
27154-27162 |
VBD |
denotes |
analyzed |
T7267 |
27151-27153 |
PRP |
denotes |
we |
T7268 |
27163-27170 |
JJ |
denotes |
primary |
T7269 |
27171-27181 |
NNS |
denotes |
astrocytes |
T7270 |
27182-27186 |
IN |
denotes |
from |
T7271 |
27187-27189 |
DT |
denotes |
an |
T7272 |
27199-27204 |
NN |
denotes |
mouse |
T7273 |
27190-27195 |
NN |
denotes |
AnxA7 |
T7274 |
27195-27196 |
SYM |
denotes |
- |
T7275 |
27196-27197 |
HYPH |
denotes |
/ |
T7276 |
27197-27198 |
SYM |
denotes |
- |
T7277 |
27205-27208 |
IN |
denotes |
for |
T7278 |
27209-27213 |
NN |
denotes |
Ca2+ |
T7279 |
27214-27223 |
JJ |
denotes |
dependent |
T7280 |
27213-27214 |
HYPH |
denotes |
- |
T7281 |
27234-27243 |
NNS |
denotes |
processes |
T7282 |
27224-27233 |
NN |
denotes |
signaling |
T7283 |
27243-27245 |
, |
denotes |
, |
T7284 |
27245-27247 |
PRP |
denotes |
we |
T7285 |
27254-27258 |
IN |
denotes |
that |
T7286 |
27264-27273 |
VBD |
denotes |
exhibited |
T7287 |
27259-27263 |
PRP |
denotes |
they |
T7288 |
27274-27275 |
DT |
denotes |
a |
T7289 |
27300-27308 |
NN |
denotes |
velocity |
T7290 |
27276-27289 |
RB |
denotes |
significantly |
T7291 |
27290-27299 |
VBN |
denotes |
increased |
T7292 |
27309-27311 |
IN |
denotes |
of |
T7293 |
27312-27324 |
RB |
denotes |
mechanically |
T7294 |
27325-27332 |
VBN |
denotes |
induced |
T7295 |
27349-27354 |
NNS |
denotes |
waves |
T7296 |
27333-27343 |
JJ |
denotes |
astrocytic |
T7297 |
27344-27348 |
NN |
denotes |
Ca2+ |
T7298 |
27348-27349 |
HYPH |
denotes |
- |
T7299 |
27355-27357 |
IN |
denotes |
as |
T7300 |
27358-27366 |
VBN |
denotes |
compared |
T7301 |
27367-27369 |
IN |
denotes |
to |
T7302 |
27370-27374 |
JJ |
denotes |
wild |
T7303 |
27375-27379 |
NN |
denotes |
type |
T7304 |
27380-27381 |
-LRB- |
denotes |
[ |
T7305 |
27381-27382 |
CD |
denotes |
9 |
T7306 |
27382-27383 |
-RRB- |
denotes |
] |
T7307 |
27383-27384 |
. |
denotes |
. |
T7308 |
27384-27486 |
sentence |
denotes |
This led us to propose, that Annexin A7 can act as a Ca2+-buffer and is involved in Ca2+-homeostasis. |
T7309 |
27385-27389 |
DT |
denotes |
This |
T7310 |
27390-27393 |
VBD |
denotes |
led |
T7311 |
27394-27396 |
PRP |
denotes |
us |
T7312 |
27397-27399 |
TO |
denotes |
to |
T7313 |
27400-27407 |
VB |
denotes |
propose |
T7314 |
27407-27409 |
, |
denotes |
, |
T7315 |
27409-27413 |
IN |
denotes |
that |
T7316 |
27429-27432 |
VB |
denotes |
act |
T7317 |
27414-27421 |
NN |
denotes |
Annexin |
T7318 |
27422-27424 |
NN |
denotes |
A7 |
T7319 |
27425-27428 |
MD |
denotes |
can |
T7320 |
27433-27435 |
IN |
denotes |
as |
T7321 |
27436-27437 |
DT |
denotes |
a |
T7322 |
27443-27449 |
NN |
denotes |
buffer |
T7323 |
27438-27442 |
NN |
denotes |
Ca2+ |
T7324 |
27442-27443 |
HYPH |
denotes |
- |
T7325 |
27450-27453 |
CC |
denotes |
and |
T7326 |
27454-27456 |
VBZ |
denotes |
is |
T7327 |
27457-27465 |
VBN |
denotes |
involved |
T7328 |
27466-27468 |
IN |
denotes |
in |
T7329 |
27469-27473 |
NN |
denotes |
Ca2+ |
T7330 |
27474-27485 |
NN |
denotes |
homeostasis |
T7331 |
27473-27474 |
HYPH |
denotes |
- |
T7332 |
27485-27486 |
. |
denotes |
. |
T7333 |
27486-27591 |
sentence |
denotes |
In neurons Ca2+ ions play major roles in various physiological and pathophysiological processes [20-25]. |
T7334 |
27487-27489 |
IN |
denotes |
In |
T7335 |
27508-27512 |
VBP |
denotes |
play |
T7336 |
27490-27497 |
NNS |
denotes |
neurons |
T7337 |
27498-27502 |
NN |
denotes |
Ca2+ |
T7338 |
27503-27507 |
NNS |
denotes |
ions |
T7339 |
27513-27518 |
JJ |
denotes |
major |
T7340 |
27519-27524 |
NNS |
denotes |
roles |
T7341 |
27525-27527 |
IN |
denotes |
in |
T7342 |
27528-27535 |
JJ |
denotes |
various |
T7343 |
27573-27582 |
NNS |
denotes |
processes |
T7344 |
27536-27549 |
JJ |
denotes |
physiological |
T7345 |
27550-27553 |
CC |
denotes |
and |
T7346 |
27554-27572 |
JJ |
denotes |
pathophysiological |
T7347 |
27583-27584 |
-LRB- |
denotes |
[ |
T7348 |
27584-27586 |
CD |
denotes |
20 |
T7349 |
27586-27587 |
SYM |
denotes |
- |
T7350 |
27587-27589 |
CD |
denotes |
25 |
T7351 |
27589-27590 |
-RRB- |
denotes |
] |
T7352 |
27590-27591 |
. |
denotes |
. |
T7353 |
27591-27743 |
sentence |
denotes |
One can speculate about an involvement of Annexin A7 in the regulation of these Ca2+-dependent processes, propositions that need further investigation. |
T7354 |
27592-27595 |
CD |
denotes |
One |
T7355 |
27600-27609 |
VB |
denotes |
speculate |
T7356 |
27596-27599 |
MD |
denotes |
can |
T7357 |
27610-27615 |
IN |
denotes |
about |
T7358 |
27616-27618 |
DT |
denotes |
an |
T7359 |
27619-27630 |
NN |
denotes |
involvement |
T7360 |
27631-27633 |
IN |
denotes |
of |
T7361 |
27634-27641 |
NN |
denotes |
Annexin |
T7362 |
27642-27644 |
NN |
denotes |
A7 |
T7363 |
27645-27647 |
IN |
denotes |
in |
T7364 |
27648-27651 |
DT |
denotes |
the |
T7365 |
27652-27662 |
NN |
denotes |
regulation |
T7366 |
27663-27665 |
IN |
denotes |
of |
T7367 |
27666-27671 |
DT |
denotes |
these |
T7368 |
27687-27696 |
NNS |
denotes |
processes |
T7369 |
27672-27676 |
NN |
denotes |
Ca2+ |
T7370 |
27677-27686 |
JJ |
denotes |
dependent |
T7371 |
27676-27677 |
HYPH |
denotes |
- |
T7372 |
27696-27698 |
, |
denotes |
, |
T7373 |
27698-27710 |
NNS |
denotes |
propositions |
T7374 |
27711-27715 |
WDT |
denotes |
that |
T7375 |
27716-27720 |
VBP |
denotes |
need |
T7376 |
27721-27728 |
JJ |
denotes |
further |
T7377 |
27729-27742 |
NN |
denotes |
investigation |
T7378 |
27742-27743 |
. |
denotes |
. |
T7379 |
27743-27963 |
sentence |
denotes |
However, such roles are confirmed for heart function by studies of Schrickel et al. [submitted], who described an involvement of Annexin A7 in the maintenance of a regular cardiac electrophysiology and Ca2+-homeostasis. |
T7380 |
27744-27751 |
RB |
denotes |
However |
T7381 |
27768-27777 |
VBN |
denotes |
confirmed |
T7382 |
27751-27753 |
, |
denotes |
, |
T7383 |
27753-27757 |
JJ |
denotes |
such |
T7384 |
27758-27763 |
NNS |
denotes |
roles |
T7385 |
27764-27767 |
VBP |
denotes |
are |
T7386 |
27778-27781 |
IN |
denotes |
for |
T7387 |
27782-27787 |
NN |
denotes |
heart |
T7388 |
27788-27796 |
NN |
denotes |
function |
T7389 |
27797-27799 |
IN |
denotes |
by |
T7390 |
27800-27807 |
NNS |
denotes |
studies |
T7391 |
27808-27810 |
IN |
denotes |
of |
T7392 |
27811-27820 |
NNP |
denotes |
Schrickel |
T7393 |
27821-27823 |
FW |
denotes |
et |
T7394 |
27824-27827 |
FW |
denotes |
al. |
T7395 |
27828-27829 |
-LRB- |
denotes |
[ |
T7396 |
27829-27838 |
VBN |
denotes |
submitted |
T7397 |
27838-27839 |
-RRB- |
denotes |
] |
T7398 |
27839-27841 |
, |
denotes |
, |
T7399 |
27841-27844 |
WP |
denotes |
who |
T7400 |
27845-27854 |
VBD |
denotes |
described |
T7401 |
27855-27857 |
DT |
denotes |
an |
T7402 |
27858-27869 |
NN |
denotes |
involvement |
T7403 |
27870-27872 |
IN |
denotes |
of |
T7404 |
27873-27880 |
NN |
denotes |
Annexin |
T7405 |
27881-27883 |
NN |
denotes |
A7 |
T7406 |
27884-27886 |
IN |
denotes |
in |
T7407 |
27887-27890 |
DT |
denotes |
the |
T7408 |
27891-27902 |
NN |
denotes |
maintenance |
T7409 |
27903-27905 |
IN |
denotes |
of |
T7410 |
27906-27907 |
DT |
denotes |
a |
T7411 |
27924-27941 |
NN |
denotes |
electrophysiology |
T7412 |
27908-27915 |
JJ |
denotes |
regular |
T7413 |
27916-27923 |
JJ |
denotes |
cardiac |
T7414 |
27942-27945 |
CC |
denotes |
and |
T7415 |
27946-27950 |
NN |
denotes |
Ca2+ |
T7416 |
27951-27962 |
NN |
denotes |
homeostasis |
T7417 |
27950-27951 |
HYPH |
denotes |
- |
T7418 |
27962-27963 |
. |
denotes |
. |
T7419 |
27963-28051 |
sentence |
denotes |
An altered subcellular location during embryogenesis was also reported for Annexin A11. |
T7420 |
27964-27966 |
DT |
denotes |
An |
T7421 |
27987-27995 |
NN |
denotes |
location |
T7422 |
27967-27974 |
VBN |
denotes |
altered |
T7423 |
27975-27986 |
JJ |
denotes |
subcellular |
T7424 |
28026-28034 |
VBN |
denotes |
reported |
T7425 |
27996-28002 |
IN |
denotes |
during |
T7426 |
28003-28016 |
NN |
denotes |
embryogenesis |
T7427 |
28017-28020 |
VBD |
denotes |
was |
T7428 |
28021-28025 |
RB |
denotes |
also |
T7429 |
28035-28038 |
IN |
denotes |
for |
T7430 |
28039-28046 |
NN |
denotes |
Annexin |
T7431 |
28047-28050 |
NN |
denotes |
A11 |
T7432 |
28050-28051 |
. |
denotes |
. |
T7433 |
28051-28247 |
sentence |
denotes |
The developing gray matter of the rat embryonic spinal cord exhibited primarily nuclear localization of Annexin A11, while immunoreactivity was lost from the nuclei in the adult spinal cord [26]. |
T7434 |
28052-28055 |
DT |
denotes |
The |
T7435 |
28072-28078 |
NN |
denotes |
matter |
T7436 |
28056-28066 |
VBG |
denotes |
developing |
T7437 |
28067-28071 |
JJ |
denotes |
gray |
T7438 |
28112-28121 |
VBD |
denotes |
exhibited |
T7439 |
28079-28081 |
IN |
denotes |
of |
T7440 |
28082-28085 |
DT |
denotes |
the |
T7441 |
28107-28111 |
NN |
denotes |
cord |
T7442 |
28086-28089 |
NN |
denotes |
rat |
T7443 |
28090-28099 |
JJ |
denotes |
embryonic |
T7444 |
28100-28106 |
JJ |
denotes |
spinal |
T7445 |
28122-28131 |
RB |
denotes |
primarily |
T7446 |
28132-28139 |
JJ |
denotes |
nuclear |
T7447 |
28140-28152 |
NN |
denotes |
localization |
T7448 |
28153-28155 |
IN |
denotes |
of |
T7449 |
28156-28163 |
NN |
denotes |
Annexin |
T7450 |
28164-28167 |
NN |
denotes |
A11 |
T7451 |
28167-28169 |
, |
denotes |
, |
T7452 |
28169-28174 |
IN |
denotes |
while |
T7453 |
28196-28200 |
VBN |
denotes |
lost |
T7454 |
28175-28191 |
NN |
denotes |
immunoreactivity |
T7455 |
28192-28195 |
VBD |
denotes |
was |
T7456 |
28201-28205 |
IN |
denotes |
from |
T7457 |
28206-28209 |
DT |
denotes |
the |
T7458 |
28210-28216 |
NNS |
denotes |
nuclei |
T7459 |
28217-28219 |
IN |
denotes |
in |
T7460 |
28220-28223 |
DT |
denotes |
the |
T7461 |
28237-28241 |
NN |
denotes |
cord |
T7462 |
28224-28229 |
JJ |
denotes |
adult |
T7463 |
28230-28236 |
JJ |
denotes |
spinal |
T7464 |
28242-28243 |
-LRB- |
denotes |
[ |
T7465 |
28243-28245 |
CD |
denotes |
26 |
T7466 |
28245-28246 |
-RRB- |
denotes |
] |
T7467 |
28246-28247 |
. |
denotes |
. |
T7468 |
28247-28379 |
sentence |
denotes |
In contrast, our studies show a relocation of Annexin A7 from the cytosol to the nucleus in cells of the embryonic neuronal tissue. |
T7469 |
28248-28250 |
IN |
denotes |
In |
T7470 |
28273-28277 |
VBP |
denotes |
show |
T7471 |
28251-28259 |
NN |
denotes |
contrast |
T7472 |
28259-28261 |
, |
denotes |
, |
T7473 |
28261-28264 |
PRP$ |
denotes |
our |
T7474 |
28265-28272 |
NNS |
denotes |
studies |
T7475 |
28278-28279 |
DT |
denotes |
a |
T7476 |
28280-28290 |
NN |
denotes |
relocation |
T7477 |
28291-28293 |
IN |
denotes |
of |
T7478 |
28294-28301 |
NN |
denotes |
Annexin |
T7479 |
28302-28304 |
NN |
denotes |
A7 |
T7480 |
28305-28309 |
IN |
denotes |
from |
T7481 |
28310-28313 |
DT |
denotes |
the |
T7482 |
28314-28321 |
NN |
denotes |
cytosol |
T7483 |
28322-28324 |
IN |
denotes |
to |
T7484 |
28325-28328 |
DT |
denotes |
the |
T7485 |
28329-28336 |
NN |
denotes |
nucleus |
T7486 |
28337-28339 |
IN |
denotes |
in |
T7487 |
28340-28345 |
NNS |
denotes |
cells |
T7488 |
28346-28348 |
IN |
denotes |
of |
T7489 |
28349-28352 |
DT |
denotes |
the |
T7490 |
28372-28378 |
NN |
denotes |
tissue |
T7491 |
28353-28362 |
JJ |
denotes |
embryonic |
T7492 |
28363-28371 |
JJ |
denotes |
neuronal |
T7493 |
28378-28379 |
. |
denotes |
. |
T7494 |
28379-28446 |
sentence |
denotes |
The Annexin A7 distribution is determined by a variety of factors. |
T7495 |
28380-28383 |
DT |
denotes |
The |
T7496 |
28395-28407 |
NN |
denotes |
distribution |
T7497 |
28384-28391 |
NN |
denotes |
Annexin |
T7498 |
28392-28394 |
NN |
denotes |
A7 |
T7499 |
28411-28421 |
VBN |
denotes |
determined |
T7500 |
28408-28410 |
VBZ |
denotes |
is |
T7501 |
28422-28424 |
IN |
denotes |
by |
T7502 |
28425-28426 |
DT |
denotes |
a |
T7503 |
28427-28434 |
NN |
denotes |
variety |
T7504 |
28435-28437 |
IN |
denotes |
of |
T7505 |
28438-28445 |
NNS |
denotes |
factors |
T7506 |
28445-28446 |
. |
denotes |
. |
T7507 |
28446-28579 |
sentence |
denotes |
An important one appears to be Ca2+, which promotes binding of Annexin A7 to membranes and also allows aggregation of annexins [27]. |
T7508 |
28447-28449 |
DT |
denotes |
An |
T7509 |
28460-28463 |
NN |
denotes |
one |
T7510 |
28450-28459 |
JJ |
denotes |
important |
T7511 |
28464-28471 |
VBZ |
denotes |
appears |
T7512 |
28472-28474 |
TO |
denotes |
to |
T7513 |
28475-28477 |
VB |
denotes |
be |
T7514 |
28478-28482 |
NN |
denotes |
Ca2+ |
T7515 |
28482-28484 |
, |
denotes |
, |
T7516 |
28484-28489 |
WDT |
denotes |
which |
T7517 |
28490-28498 |
VBZ |
denotes |
promotes |
T7518 |
28499-28506 |
NN |
denotes |
binding |
T7519 |
28507-28509 |
IN |
denotes |
of |
T7520 |
28510-28517 |
NN |
denotes |
Annexin |
T7521 |
28518-28520 |
NN |
denotes |
A7 |
T7522 |
28521-28523 |
IN |
denotes |
to |
T7523 |
28524-28533 |
NNS |
denotes |
membranes |
T7524 |
28534-28537 |
CC |
denotes |
and |
T7525 |
28538-28542 |
RB |
denotes |
also |
T7526 |
28543-28549 |
VBZ |
denotes |
allows |
T7527 |
28550-28561 |
NN |
denotes |
aggregation |
T7528 |
28562-28564 |
IN |
denotes |
of |
T7529 |
28565-28573 |
NNS |
denotes |
annexins |
T7530 |
28574-28575 |
-LRB- |
denotes |
[ |
T7531 |
28575-28577 |
CD |
denotes |
27 |
T7532 |
28577-28578 |
-RRB- |
denotes |
] |
T7533 |
28578-28579 |
. |
denotes |
. |
T7534 |
28579-28666 |
sentence |
denotes |
Binding partners of Annexin A7 such as sorcin might represent additional factors [28]. |
T7535 |
28580-28587 |
NN |
denotes |
Binding |
T7536 |
28588-28596 |
NNS |
denotes |
partners |
T7537 |
28632-28641 |
VB |
denotes |
represent |
T7538 |
28597-28599 |
IN |
denotes |
of |
T7539 |
28600-28607 |
NN |
denotes |
Annexin |
T7540 |
28608-28610 |
NN |
denotes |
A7 |
T7541 |
28611-28615 |
JJ |
denotes |
such |
T7542 |
28616-28618 |
IN |
denotes |
as |
T7543 |
28619-28625 |
NN |
denotes |
sorcin |
T7544 |
28626-28631 |
MD |
denotes |
might |
T7545 |
28642-28652 |
JJ |
denotes |
additional |
T7546 |
28653-28660 |
NNS |
denotes |
factors |
T7547 |
28661-28662 |
-LRB- |
denotes |
[ |
T7548 |
28662-28664 |
CD |
denotes |
28 |
T7549 |
28664-28665 |
-RRB- |
denotes |
] |
T7550 |
28665-28666 |
. |
denotes |
. |
T7551 |
28666-28925 |
sentence |
denotes |
Although the members of the annexin family are generally found at the plasma membrane, in the cytoplasm or in association with the cytoskeleton, Annexins A1, A2, A4, A5 and A11 have been described to be localized at least partially in the nucleus [26,29,30]. |
T7552 |
28667-28675 |
IN |
denotes |
Although |
T7553 |
28724-28729 |
VBN |
denotes |
found |
T7554 |
28676-28679 |
DT |
denotes |
the |
T7555 |
28680-28687 |
NNS |
denotes |
members |
T7556 |
28688-28690 |
IN |
denotes |
of |
T7557 |
28691-28694 |
DT |
denotes |
the |
T7558 |
28703-28709 |
NN |
denotes |
family |
T7559 |
28695-28702 |
NN |
denotes |
annexin |
T7560 |
28710-28713 |
VBP |
denotes |
are |
T7561 |
28714-28723 |
RB |
denotes |
generally |
T7562 |
28854-28863 |
VBN |
denotes |
described |
T7563 |
28730-28732 |
IN |
denotes |
at |
T7564 |
28733-28736 |
DT |
denotes |
the |
T7565 |
28744-28752 |
NN |
denotes |
membrane |
T7566 |
28737-28743 |
NN |
denotes |
plasma |
T7567 |
28752-28754 |
, |
denotes |
, |
T7568 |
28754-28756 |
IN |
denotes |
in |
T7569 |
28757-28760 |
DT |
denotes |
the |
T7570 |
28761-28770 |
NN |
denotes |
cytoplasm |
T7571 |
28771-28773 |
CC |
denotes |
or |
T7572 |
28774-28776 |
IN |
denotes |
in |
T7573 |
28777-28788 |
NN |
denotes |
association |
T7574 |
28789-28793 |
IN |
denotes |
with |
T7575 |
28794-28797 |
DT |
denotes |
the |
T7576 |
28798-28810 |
NN |
denotes |
cytoskeleton |
T7577 |
28810-28812 |
, |
denotes |
, |
T7578 |
28812-28820 |
NNS |
denotes |
Annexins |
T7579 |
28821-28823 |
NN |
denotes |
A1 |
T7580 |
28823-28825 |
, |
denotes |
, |
T7581 |
28825-28827 |
NN |
denotes |
A2 |
T7582 |
28827-28829 |
, |
denotes |
, |
T7583 |
28829-28831 |
NN |
denotes |
A4 |
T7584 |
28831-28833 |
, |
denotes |
, |
T7585 |
28833-28835 |
NN |
denotes |
A5 |
T7586 |
28836-28839 |
CC |
denotes |
and |
T7587 |
28840-28843 |
NN |
denotes |
A11 |
T7588 |
28844-28848 |
VBP |
denotes |
have |
T7589 |
28849-28853 |
VBN |
denotes |
been |
T7590 |
28864-28866 |
TO |
denotes |
to |
T7591 |
28870-28879 |
VBN |
denotes |
localized |
T7592 |
28867-28869 |
VB |
denotes |
be |
T7593 |
28880-28882 |
RB |
denotes |
at |
T7594 |
28883-28888 |
RBS |
denotes |
least |
T7595 |
28889-28898 |
RB |
denotes |
partially |
T7596 |
28899-28901 |
IN |
denotes |
in |
T7597 |
28902-28905 |
DT |
denotes |
the |
T7598 |
28906-28913 |
NN |
denotes |
nucleus |
T7599 |
28914-28915 |
-LRB- |
denotes |
[ |
T7600 |
28921-28923 |
CD |
denotes |
30 |
T7601 |
28915-28917 |
CD |
denotes |
26 |
T7602 |
28917-28918 |
, |
denotes |
, |
T7603 |
28918-28920 |
CD |
denotes |
29 |
T7604 |
28920-28921 |
, |
denotes |
, |
T7605 |
28923-28924 |
-RRB- |
denotes |
] |
T7606 |
28924-28925 |
. |
denotes |
. |
T7607 |
28925-29086 |
sentence |
denotes |
Studies with human foreskin fibroblasts demonstrated that Annexin A1, A4 and A5 are all present in the nucleus at higher concentration than in the cytosol [31]. |
T7608 |
28926-28933 |
NNS |
denotes |
Studies |
T7609 |
28966-28978 |
VBD |
denotes |
demonstrated |
T7610 |
28934-28938 |
IN |
denotes |
with |
T7611 |
28939-28944 |
JJ |
denotes |
human |
T7612 |
28954-28965 |
NNS |
denotes |
fibroblasts |
T7613 |
28945-28953 |
NN |
denotes |
foreskin |
T7614 |
28979-28983 |
IN |
denotes |
that |
T7615 |
29006-29009 |
VBP |
denotes |
are |
T7616 |
28984-28991 |
NN |
denotes |
Annexin |
T7617 |
28992-28994 |
NN |
denotes |
A1 |
T7618 |
28994-28996 |
, |
denotes |
, |
T7619 |
28996-28998 |
NN |
denotes |
A4 |
T7620 |
28999-29002 |
CC |
denotes |
and |
T7621 |
29003-29005 |
NN |
denotes |
A5 |
T7622 |
29010-29013 |
RB |
denotes |
all |
T7623 |
29014-29021 |
JJ |
denotes |
present |
T7624 |
29022-29024 |
IN |
denotes |
in |
T7625 |
29025-29028 |
DT |
denotes |
the |
T7626 |
29029-29036 |
NN |
denotes |
nucleus |
T7627 |
29037-29039 |
IN |
denotes |
at |
T7628 |
29040-29046 |
JJR |
denotes |
higher |
T7629 |
29047-29060 |
NN |
denotes |
concentration |
T7630 |
29061-29065 |
IN |
denotes |
than |
T7631 |
29066-29068 |
IN |
denotes |
in |
T7632 |
29069-29072 |
DT |
denotes |
the |
T7633 |
29073-29080 |
NN |
denotes |
cytosol |
T7634 |
29081-29082 |
-LRB- |
denotes |
[ |
T7635 |
29082-29084 |
CD |
denotes |
31 |
T7636 |
29084-29085 |
-RRB- |
denotes |
] |
T7637 |
29085-29086 |
. |
denotes |
. |
T7638 |
29086-29174 |
sentence |
denotes |
Raising intracellular Ca2+ led to relocation of these annexins to the nuclear membrane. |
T7639 |
29087-29094 |
VBG |
denotes |
Raising |
T7640 |
29114-29117 |
VBD |
denotes |
led |
T7641 |
29095-29108 |
JJ |
denotes |
intracellular |
T7642 |
29109-29113 |
NN |
denotes |
Ca2+ |
T7643 |
29118-29120 |
IN |
denotes |
to |
T7644 |
29121-29131 |
NN |
denotes |
relocation |
T7645 |
29132-29134 |
IN |
denotes |
of |
T7646 |
29135-29140 |
DT |
denotes |
these |
T7647 |
29141-29149 |
NNS |
denotes |
annexins |
T7648 |
29150-29152 |
IN |
denotes |
to |
T7649 |
29153-29156 |
DT |
denotes |
the |
T7650 |
29165-29173 |
NN |
denotes |
membrane |
T7651 |
29157-29164 |
JJ |
denotes |
nuclear |
T7652 |
29173-29174 |
. |
denotes |
. |
T7653 |
29174-29285 |
sentence |
denotes |
An important role for annexins in mediating the Ca2+-signal within the nuclei of the fibroblasts was proposed. |
T7654 |
29175-29177 |
DT |
denotes |
An |
T7655 |
29188-29192 |
NN |
denotes |
role |
T7656 |
29178-29187 |
JJ |
denotes |
important |
T7657 |
29276-29284 |
VBN |
denotes |
proposed |
T7658 |
29193-29196 |
IN |
denotes |
for |
T7659 |
29197-29205 |
NNS |
denotes |
annexins |
T7660 |
29206-29208 |
IN |
denotes |
in |
T7661 |
29209-29218 |
VBG |
denotes |
mediating |
T7662 |
29219-29222 |
DT |
denotes |
the |
T7663 |
29228-29234 |
NN |
denotes |
signal |
T7664 |
29223-29227 |
NN |
denotes |
Ca2+ |
T7665 |
29227-29228 |
HYPH |
denotes |
- |
T7666 |
29235-29241 |
IN |
denotes |
within |
T7667 |
29242-29245 |
DT |
denotes |
the |
T7668 |
29246-29252 |
NNS |
denotes |
nuclei |
T7669 |
29253-29255 |
IN |
denotes |
of |
T7670 |
29256-29259 |
DT |
denotes |
the |
T7671 |
29260-29271 |
NNS |
denotes |
fibroblasts |
T7672 |
29272-29275 |
VBD |
denotes |
was |
T7673 |
29284-29285 |
. |
denotes |
. |
T7674 |
29285-29513 |
sentence |
denotes |
These results mirror studies with stably transfected C6 cells, in which high intracellular Ca2+-concentrations induced a marked redistribution of Annexin A7 from its localization in the nucleoplasm to the nuclear membrane [19]. |
T7675 |
29286-29291 |
DT |
denotes |
These |
T7676 |
29292-29299 |
NNS |
denotes |
results |
T7677 |
29300-29306 |
VBP |
denotes |
mirror |
T7678 |
29307-29314 |
NNS |
denotes |
studies |
T7679 |
29315-29319 |
IN |
denotes |
with |
T7680 |
29320-29326 |
RB |
denotes |
stably |
T7681 |
29327-29338 |
VBN |
denotes |
transfected |
T7682 |
29342-29347 |
NNS |
denotes |
cells |
T7683 |
29339-29341 |
NN |
denotes |
C6 |
T7684 |
29347-29349 |
, |
denotes |
, |
T7685 |
29349-29351 |
IN |
denotes |
in |
T7686 |
29397-29404 |
VBD |
denotes |
induced |
T7687 |
29352-29357 |
WDT |
denotes |
which |
T7688 |
29358-29362 |
JJ |
denotes |
high |
T7689 |
29382-29396 |
NNS |
denotes |
concentrations |
T7690 |
29363-29376 |
JJ |
denotes |
intracellular |
T7691 |
29377-29381 |
NN |
denotes |
Ca2+ |
T7692 |
29381-29382 |
HYPH |
denotes |
- |
T7693 |
29405-29406 |
DT |
denotes |
a |
T7694 |
29414-29428 |
NN |
denotes |
redistribution |
T7695 |
29407-29413 |
JJ |
denotes |
marked |
T7696 |
29429-29431 |
IN |
denotes |
of |
T7697 |
29432-29439 |
NN |
denotes |
Annexin |
T7698 |
29440-29442 |
NN |
denotes |
A7 |
T7699 |
29443-29447 |
IN |
denotes |
from |
T7700 |
29448-29451 |
PRP$ |
denotes |
its |
T7701 |
29452-29464 |
NN |
denotes |
localization |
T7702 |
29465-29467 |
IN |
denotes |
in |
T7703 |
29468-29471 |
DT |
denotes |
the |
T7704 |
29472-29483 |
NN |
denotes |
nucleoplasm |
T7705 |
29484-29486 |
IN |
denotes |
to |
T7706 |
29487-29490 |
DT |
denotes |
the |
T7707 |
29499-29507 |
NN |
denotes |
membrane |
T7708 |
29491-29498 |
JJ |
denotes |
nuclear |
T7709 |
29508-29509 |
-LRB- |
denotes |
[ |
T7710 |
29509-29511 |
CD |
denotes |
19 |
T7711 |
29511-29512 |
-RRB- |
denotes |
] |
T7712 |
29512-29513 |
. |
denotes |
. |
T7713 |
29513-29645 |
sentence |
denotes |
None of the annexins contains a typical nuclear localization signal and their mechanism of nuclear import remains to be elucidated. |
T7714 |
29514-29518 |
NN |
denotes |
None |
T7715 |
29535-29543 |
VBZ |
denotes |
contains |
T7716 |
29519-29521 |
IN |
denotes |
of |
T7717 |
29522-29525 |
DT |
denotes |
the |
T7718 |
29526-29534 |
NNS |
denotes |
annexins |
T7719 |
29544-29545 |
DT |
denotes |
a |
T7720 |
29575-29581 |
NN |
denotes |
signal |
T7721 |
29546-29553 |
JJ |
denotes |
typical |
T7722 |
29554-29561 |
JJ |
denotes |
nuclear |
T7723 |
29562-29574 |
NN |
denotes |
localization |
T7724 |
29582-29585 |
CC |
denotes |
and |
T7725 |
29586-29591 |
PRP$ |
denotes |
their |
T7726 |
29592-29601 |
NN |
denotes |
mechanism |
T7727 |
29620-29627 |
VBZ |
denotes |
remains |
T7728 |
29602-29604 |
IN |
denotes |
of |
T7729 |
29605-29612 |
JJ |
denotes |
nuclear |
T7730 |
29613-29619 |
NN |
denotes |
import |
T7731 |
29628-29630 |
TO |
denotes |
to |
T7732 |
29634-29644 |
VBN |
denotes |
elucidated |
T7733 |
29631-29633 |
VB |
denotes |
be |
T7734 |
29644-29645 |
. |
denotes |
. |
T7735 |
29645-29810 |
sentence |
denotes |
For Annexin A11 it was shown that nuclear localization is mediated by its N-terminal region, which also contains a binding side for the S100 protein calcyclin [29]. |
T7736 |
29646-29649 |
IN |
denotes |
For |
T7737 |
29669-29674 |
VBN |
denotes |
shown |
T7738 |
29650-29657 |
NN |
denotes |
Annexin |
T7739 |
29658-29661 |
NN |
denotes |
A11 |
T7740 |
29662-29664 |
PRP |
denotes |
it |
T7741 |
29665-29668 |
VBD |
denotes |
was |
T7742 |
29675-29679 |
IN |
denotes |
that |
T7743 |
29704-29712 |
VBN |
denotes |
mediated |
T7744 |
29680-29687 |
JJ |
denotes |
nuclear |
T7745 |
29688-29700 |
NN |
denotes |
localization |
T7746 |
29701-29703 |
VBZ |
denotes |
is |
T7747 |
29713-29715 |
IN |
denotes |
by |
T7748 |
29716-29719 |
PRP$ |
denotes |
its |
T7749 |
29731-29737 |
NN |
denotes |
region |
T7750 |
29720-29721 |
NN |
denotes |
N |
T7751 |
29722-29730 |
JJ |
denotes |
terminal |
T7752 |
29721-29722 |
HYPH |
denotes |
- |
T7753 |
29737-29739 |
, |
denotes |
, |
T7754 |
29739-29744 |
WDT |
denotes |
which |
T7755 |
29750-29758 |
VBZ |
denotes |
contains |
T7756 |
29745-29749 |
RB |
denotes |
also |
T7757 |
29759-29760 |
DT |
denotes |
a |
T7758 |
29769-29773 |
NN |
denotes |
side |
T7759 |
29761-29768 |
NN |
denotes |
binding |
T7760 |
29774-29777 |
IN |
denotes |
for |
T7761 |
29778-29781 |
DT |
denotes |
the |
T7762 |
29787-29794 |
NN |
denotes |
protein |
T7763 |
29782-29786 |
NN |
denotes |
S100 |
T7764 |
29795-29804 |
NN |
denotes |
calcyclin |
T7765 |
29805-29806 |
-LRB- |
denotes |
[ |
T7766 |
29806-29808 |
CD |
denotes |
29 |
T7767 |
29808-29809 |
-RRB- |
denotes |
] |
T7768 |
29809-29810 |
. |
denotes |
. |
T7769 |
29810-29939 |
sentence |
denotes |
More recently Tomas and Moss [32] showed, that Annexin A11 and S100A6 assemble at the nuclear envelope during nuclear breakdown. |
T7770 |
29811-29815 |
RBR |
denotes |
More |
T7771 |
29816-29824 |
RB |
denotes |
recently |
T7772 |
29845-29851 |
VBD |
denotes |
showed |
T7773 |
29825-29830 |
NNP |
denotes |
Tomas |
T7774 |
29831-29834 |
CC |
denotes |
and |
T7775 |
29835-29839 |
NNP |
denotes |
Moss |
T7776 |
29840-29841 |
-LRB- |
denotes |
[ |
T7777 |
29841-29843 |
CD |
denotes |
32 |
T7778 |
29843-29844 |
-RRB- |
denotes |
] |
T7779 |
29851-29853 |
, |
denotes |
, |
T7780 |
29853-29857 |
IN |
denotes |
that |
T7781 |
29881-29889 |
VBP |
denotes |
assemble |
T7782 |
29858-29865 |
NN |
denotes |
Annexin |
T7783 |
29866-29869 |
NN |
denotes |
A11 |
T7784 |
29870-29873 |
CC |
denotes |
and |
T7785 |
29874-29880 |
NN |
denotes |
S100A6 |
T7786 |
29890-29892 |
IN |
denotes |
at |
T7787 |
29893-29896 |
DT |
denotes |
the |
T7788 |
29905-29913 |
NN |
denotes |
envelope |
T7789 |
29897-29904 |
JJ |
denotes |
nuclear |
T7790 |
29914-29920 |
IN |
denotes |
during |
T7791 |
29921-29928 |
JJ |
denotes |
nuclear |
T7792 |
29929-29938 |
NN |
denotes |
breakdown |
T7793 |
29938-29939 |
. |
denotes |
. |
T7794 |
29939-29980 |
sentence |
denotes |
Their role in this process is not known. |
T7795 |
29940-29945 |
PRP$ |
denotes |
Their |
T7796 |
29946-29950 |
NN |
denotes |
role |
T7797 |
29974-29979 |
VBN |
denotes |
known |
T7798 |
29951-29953 |
IN |
denotes |
in |
T7799 |
29954-29958 |
DT |
denotes |
this |
T7800 |
29959-29966 |
NN |
denotes |
process |
T7801 |
29967-29969 |
VBZ |
denotes |
is |
T7802 |
29970-29973 |
RB |
denotes |
not |
T7803 |
29979-29980 |
. |
denotes |
. |
T7804 |
29980-30069 |
sentence |
denotes |
In general it seems that the nuclear localization of the annexins is actively regulated. |
T7805 |
29981-29983 |
IN |
denotes |
In |
T7806 |
29995-30000 |
VBZ |
denotes |
seems |
T7807 |
29984-29991 |
JJ |
denotes |
general |
T7808 |
29992-29994 |
PRP |
denotes |
it |
T7809 |
30001-30005 |
IN |
denotes |
that |
T7810 |
30059-30068 |
VBN |
denotes |
regulated |
T7811 |
30006-30009 |
DT |
denotes |
the |
T7812 |
30018-30030 |
NN |
denotes |
localization |
T7813 |
30010-30017 |
JJ |
denotes |
nuclear |
T7814 |
30031-30033 |
IN |
denotes |
of |
T7815 |
30034-30037 |
DT |
denotes |
the |
T7816 |
30038-30046 |
NNS |
denotes |
annexins |
T7817 |
30047-30049 |
VBZ |
denotes |
is |
T7818 |
30050-30058 |
RB |
denotes |
actively |
T7819 |
30068-30069 |
. |
denotes |
. |
T7820 |
30069-30291 |
sentence |
denotes |
For example the nucleocytoplasmic compartmentalization of Annexin A2 is controlled by sequestration of the AnxA2/p11 complex modulated by phosphorylation and by a nuclear export signal found in the AnxA2 3–12 region [33]. |
T7821 |
30070-30073 |
IN |
denotes |
For |
T7822 |
30142-30152 |
VBN |
denotes |
controlled |
T7823 |
30074-30081 |
NN |
denotes |
example |
T7824 |
30082-30085 |
DT |
denotes |
the |
T7825 |
30104-30124 |
NN |
denotes |
compartmentalization |
T7826 |
30086-30103 |
JJ |
denotes |
nucleocytoplasmic |
T7827 |
30125-30127 |
IN |
denotes |
of |
T7828 |
30128-30135 |
NN |
denotes |
Annexin |
T7829 |
30136-30138 |
NN |
denotes |
A2 |
T7830 |
30139-30141 |
VBZ |
denotes |
is |
T7831 |
30153-30155 |
IN |
denotes |
by |
T7832 |
30156-30169 |
NN |
denotes |
sequestration |
T7833 |
30170-30172 |
IN |
denotes |
of |
T7834 |
30173-30176 |
DT |
denotes |
the |
T7835 |
30187-30194 |
NN |
denotes |
complex |
T7836 |
30177-30182 |
NN |
denotes |
AnxA2 |
T7837 |
30183-30186 |
NN |
denotes |
p11 |
T7838 |
30182-30183 |
HYPH |
denotes |
/ |
T7839 |
30195-30204 |
VBN |
denotes |
modulated |
T7840 |
30205-30207 |
IN |
denotes |
by |
T7841 |
30208-30223 |
NN |
denotes |
phosphorylation |
T7842 |
30224-30227 |
CC |
denotes |
and |
T7843 |
30228-30230 |
IN |
denotes |
by |
T7844 |
30231-30232 |
DT |
denotes |
a |
T7845 |
30248-30254 |
NN |
denotes |
signal |
T7846 |
30233-30240 |
JJ |
denotes |
nuclear |
T7847 |
30241-30247 |
NN |
denotes |
export |
T7848 |
30255-30260 |
VBN |
denotes |
found |
T7849 |
30261-30263 |
IN |
denotes |
in |
T7850 |
30264-30267 |
DT |
denotes |
the |
T7851 |
30279-30285 |
NN |
denotes |
region |
T7852 |
30268-30273 |
NN |
denotes |
AnxA2 |
T7853 |
30274-30275 |
CD |
denotes |
3 |
T7854 |
30275-30276 |
SYM |
denotes |
– |
T7855 |
30276-30278 |
CD |
denotes |
12 |
T7856 |
30286-30287 |
-LRB- |
denotes |
[ |
T7857 |
30287-30289 |
CD |
denotes |
33 |
T7858 |
30289-30290 |
-RRB- |
denotes |
] |
T7859 |
30290-30291 |
. |
denotes |
. |
T7860 |
30291-30519 |
sentence |
denotes |
One function of Annexin A2 in the nucleus, that appears not to involve binding of p11, has been suggested by its purification as part of a primer recognition protein complex that enhances DNA polymeraseα-activity in vitro [34]. |
T7861 |
30292-30295 |
CD |
denotes |
One |
T7862 |
30296-30304 |
NN |
denotes |
function |
T7863 |
30388-30397 |
VBN |
denotes |
suggested |
T7864 |
30305-30307 |
IN |
denotes |
of |
T7865 |
30308-30315 |
NN |
denotes |
Annexin |
T7866 |
30316-30318 |
NN |
denotes |
A2 |
T7867 |
30319-30321 |
IN |
denotes |
in |
T7868 |
30322-30325 |
DT |
denotes |
the |
T7869 |
30326-30333 |
NN |
denotes |
nucleus |
T7870 |
30333-30335 |
, |
denotes |
, |
T7871 |
30335-30339 |
WDT |
denotes |
that |
T7872 |
30340-30347 |
VBZ |
denotes |
appears |
T7873 |
30348-30351 |
RB |
denotes |
not |
T7874 |
30355-30362 |
VB |
denotes |
involve |
T7875 |
30352-30354 |
TO |
denotes |
to |
T7876 |
30363-30370 |
NN |
denotes |
binding |
T7877 |
30371-30373 |
IN |
denotes |
of |
T7878 |
30374-30377 |
NN |
denotes |
p11 |
T7879 |
30377-30379 |
, |
denotes |
, |
T7880 |
30379-30382 |
VBZ |
denotes |
has |
T7881 |
30383-30387 |
VBN |
denotes |
been |
T7888 |
30429-30430 |
DT |
denotes |
a |
T7889 |
30458-30465 |
NN |
denotes |
complex |
T7890 |
30431-30437 |
NN |
denotes |
primer |
T7891 |
30438-30449 |
NN |
denotes |
recognition |
T7892 |
30450-30457 |
NN |
denotes |
protein |
T7893 |
30466-30470 |
WDT |
denotes |
that |
T7894 |
30471-30479 |
VBZ |
denotes |
enhances |
T7895 |
30480-30483 |
NN |
denotes |
DNA |
T7896 |
30484-30495 |
NN |
denotes |
polymeraseα |
T7897 |
30496-30504 |
NN |
denotes |
activity |
T7898 |
30495-30496 |
HYPH |
denotes |
- |
T7899 |
30505-30507 |
FW |
denotes |
in |
T7900 |
30508-30513 |
FW |
denotes |
vitro |
T7901 |
30514-30515 |
-LRB- |
denotes |
[ |
T7902 |
30515-30517 |
CD |
denotes |
34 |
T7903 |
30517-30518 |
-RRB- |
denotes |
] |
T7904 |
30518-30519 |
. |
denotes |
. |
T7905 |
30519-30664 |
sentence |
denotes |
The annexins may participate in a nuclear response to initial cell stimulation or to a Ca2+-transient, presumably by regulating DNA replication. |
T7906 |
30520-30523 |
DT |
denotes |
The |
T7907 |
30524-30532 |
NNS |
denotes |
annexins |
T7908 |
30537-30548 |
VB |
denotes |
participate |
T7909 |
30533-30536 |
MD |
denotes |
may |
T7910 |
30549-30551 |
IN |
denotes |
in |
T7911 |
30552-30553 |
DT |
denotes |
a |
T7912 |
30562-30570 |
NN |
denotes |
response |
T7913 |
30554-30561 |
JJ |
denotes |
nuclear |
T7914 |
30571-30573 |
IN |
denotes |
to |
T7915 |
30574-30581 |
JJ |
denotes |
initial |
T7916 |
30587-30598 |
NN |
denotes |
stimulation |
T7917 |
30582-30586 |
NN |
denotes |
cell |
T7918 |
30599-30601 |
CC |
denotes |
or |
T7919 |
30602-30604 |
IN |
denotes |
to |
T7920 |
30605-30606 |
DT |
denotes |
a |
T7921 |
30607-30611 |
NN |
denotes |
Ca2+ |
T7922 |
30611-30612 |
HYPH |
denotes |
- |
T7923 |
30612-30621 |
JJ |
denotes |
transient |
T7924 |
30621-30623 |
, |
denotes |
, |
T7925 |
30623-30633 |
RB |
denotes |
presumably |
T7926 |
30634-30636 |
IN |
denotes |
by |
T7927 |
30637-30647 |
VBG |
denotes |
regulating |
T7928 |
30648-30651 |
NN |
denotes |
DNA |
T7929 |
30652-30663 |
NN |
denotes |
replication |
T7930 |
30663-30664 |
. |
denotes |
. |
T7931 |
30664-30729 |
sentence |
denotes |
For Annexin A7 such a pathway is very speculative at the moment. |
T7932 |
30665-30668 |
IN |
denotes |
For |
T7933 |
30695-30697 |
VBZ |
denotes |
is |
T7934 |
30669-30676 |
NN |
denotes |
Annexin |
T7935 |
30677-30679 |
NN |
denotes |
A7 |
T7936 |
30680-30684 |
PDT |
denotes |
such |
T7937 |
30687-30694 |
NN |
denotes |
pathway |
T7938 |
30685-30686 |
DT |
denotes |
a |
T7939 |
30698-30702 |
RB |
denotes |
very |
T7940 |
30703-30714 |
JJ |
denotes |
speculative |
T7941 |
30715-30717 |
IN |
denotes |
at |
T7942 |
30718-30721 |
DT |
denotes |
the |
T7943 |
30722-30728 |
NN |
denotes |
moment |
T7944 |
30728-30729 |
. |
denotes |
. |
T7945 |
30729-30940 |
sentence |
denotes |
Future studies are however directed by these findings and will concentrate on the identification of the nuclear localization signal of Annexin A7 as well as on the role of Annexin A7 in the nuclear compartment. |
T7946 |
30730-30736 |
NN |
denotes |
Future |
T7947 |
30737-30744 |
NNS |
denotes |
studies |
T7948 |
30757-30765 |
VBN |
denotes |
directed |
T7949 |
30745-30748 |
VBP |
denotes |
are |
T7950 |
30749-30756 |
RB |
denotes |
however |
T7951 |
30766-30768 |
IN |
denotes |
by |
T7952 |
30769-30774 |
DT |
denotes |
these |
T7953 |
30775-30783 |
NNS |
denotes |
findings |
T7954 |
30784-30787 |
CC |
denotes |
and |
T7955 |
30788-30792 |
MD |
denotes |
will |
T7956 |
30793-30804 |
VB |
denotes |
concentrate |
T7957 |
30805-30807 |
IN |
denotes |
on |
T7958 |
30808-30811 |
DT |
denotes |
the |
T7959 |
30812-30826 |
NN |
denotes |
identification |
T7960 |
30827-30829 |
IN |
denotes |
of |
T7961 |
30830-30833 |
DT |
denotes |
the |
T7962 |
30855-30861 |
NN |
denotes |
signal |
T7963 |
30834-30841 |
JJ |
denotes |
nuclear |
T7964 |
30842-30854 |
NN |
denotes |
localization |
T7965 |
30862-30864 |
IN |
denotes |
of |
T7966 |
30865-30872 |
NN |
denotes |
Annexin |
T7967 |
30873-30875 |
NN |
denotes |
A7 |
T7968 |
30876-30878 |
RB |
denotes |
as |
T7969 |
30884-30886 |
IN |
denotes |
as |
T7970 |
30879-30883 |
RB |
denotes |
well |
T7971 |
30887-30889 |
IN |
denotes |
on |
T7972 |
30890-30893 |
DT |
denotes |
the |
T7973 |
30894-30898 |
NN |
denotes |
role |
T7974 |
30899-30901 |
IN |
denotes |
of |
T7975 |
30902-30909 |
NN |
denotes |
Annexin |
T7976 |
30910-30912 |
NN |
denotes |
A7 |
T7977 |
30913-30915 |
IN |
denotes |
in |
T7978 |
30916-30919 |
DT |
denotes |
the |
T7979 |
30928-30939 |
NN |
denotes |
compartment |
T7980 |
30920-30927 |
JJ |
denotes |
nuclear |
T7981 |
30939-30940 |
. |
denotes |
. |
T8198 |
30953-30955 |
IN |
denotes |
In |
T8199 |
30972-30978 |
VBP |
denotes |
report |
T8200 |
30956-30960 |
DT |
denotes |
this |
T8201 |
30961-30968 |
NN |
denotes |
article |
T8202 |
30969-30971 |
PRP |
denotes |
we |
T8203 |
30979-30982 |
DT |
denotes |
the |
T8204 |
30983-30996 |
NN |
denotes |
translocation |
T8205 |
30997-30999 |
IN |
denotes |
of |
T8206 |
31000-31007 |
NN |
denotes |
Annexin |
T8207 |
31008-31010 |
NN |
denotes |
A7 |
T8208 |
31011-31013 |
IN |
denotes |
to |
T8209 |
31014-31020 |
NNS |
denotes |
nuclei |
T8210 |
31021-31023 |
IN |
denotes |
of |
T8211 |
31024-31039 |
VBG |
denotes |
differentiating |
T8212 |
31040-31045 |
NNS |
denotes |
cells |
T8213 |
31046-31048 |
IN |
denotes |
in |
T8214 |
31049-31052 |
DT |
denotes |
the |
T8215 |
31071-31076 |
NN |
denotes |
brain |
T8216 |
31053-31063 |
VBG |
denotes |
developing |
T8217 |
31064-31070 |
JJ |
denotes |
murine |
T8218 |
31076-31077 |
. |
denotes |
. |
T8219 |
31077-31152 |
sentence |
denotes |
In the adult brain Annexin A7 generally was detected in nuclei of neurons. |
T8220 |
31078-31080 |
IN |
denotes |
In |
T8221 |
31122-31130 |
VBN |
denotes |
detected |
T8222 |
31081-31084 |
DT |
denotes |
the |
T8223 |
31091-31096 |
NN |
denotes |
brain |
T8224 |
31085-31090 |
JJ |
denotes |
adult |
T8225 |
31097-31104 |
NN |
denotes |
Annexin |
T8226 |
31105-31107 |
NN |
denotes |
A7 |
T8227 |
31108-31117 |
RB |
denotes |
generally |
T8228 |
31118-31121 |
VBD |
denotes |
was |
T8229 |
31131-31133 |
IN |
denotes |
in |
T8230 |
31134-31140 |
NNS |
denotes |
nuclei |
T8231 |
31141-31143 |
IN |
denotes |
of |
T8232 |
31144-31151 |
NNS |
denotes |
neurons |
T8233 |
31151-31152 |
. |
denotes |
. |
T8234 |
31152-31285 |
sentence |
denotes |
Astrocytes, cerebellar Purkinje-cells and neocortical pyramidal neurons exhibited both, Annexin A7 in the cytosol and in the nuclei. |
T8235 |
31153-31163 |
NNS |
denotes |
Astrocytes |
T8236 |
31225-31234 |
VBD |
denotes |
exhibited |
T8237 |
31163-31165 |
, |
denotes |
, |
T8238 |
31165-31175 |
JJ |
denotes |
cerebellar |
T8239 |
31185-31190 |
NNS |
denotes |
cells |
T8240 |
31176-31184 |
NNP |
denotes |
Purkinje |
T8241 |
31184-31185 |
HYPH |
denotes |
- |
T8242 |
31191-31194 |
CC |
denotes |
and |
T8243 |
31195-31206 |
JJ |
denotes |
neocortical |
T8244 |
31217-31224 |
NNS |
denotes |
neurons |
T8245 |
31207-31216 |
JJ |
denotes |
pyramidal |
T8246 |
31235-31239 |
CC |
denotes |
both |
T8247 |
31249-31251 |
NN |
denotes |
A7 |
T8248 |
31239-31241 |
, |
denotes |
, |
T8249 |
31241-31248 |
NN |
denotes |
Annexin |
T8250 |
31252-31254 |
IN |
denotes |
in |
T8251 |
31255-31258 |
DT |
denotes |
the |
T8252 |
31259-31266 |
NN |
denotes |
cytosol |
T8253 |
31267-31270 |
CC |
denotes |
and |
T8254 |
31271-31273 |
IN |
denotes |
in |
T8255 |
31274-31277 |
DT |
denotes |
the |
T8256 |
31278-31284 |
NNS |
denotes |
nuclei |
T8257 |
31284-31285 |
. |
denotes |
. |
T8258 |
31285-31388 |
sentence |
denotes |
Thus, the subcellular localisation of Annexin A7 depends on the developmental stage and the cell type. |
T8259 |
31286-31290 |
RB |
denotes |
Thus |
T8260 |
31335-31342 |
VBZ |
denotes |
depends |
T8261 |
31290-31292 |
, |
denotes |
, |
T8262 |
31292-31295 |
DT |
denotes |
the |
T8263 |
31308-31320 |
NN |
denotes |
localisation |
T8264 |
31296-31307 |
JJ |
denotes |
subcellular |
T8265 |
31321-31323 |
IN |
denotes |
of |
T8266 |
31324-31331 |
NN |
denotes |
Annexin |
T8267 |
31332-31334 |
NN |
denotes |
A7 |
T8268 |
31343-31345 |
IN |
denotes |
on |
T8269 |
31346-31349 |
DT |
denotes |
the |
T8270 |
31364-31369 |
NN |
denotes |
stage |
T8271 |
31350-31363 |
JJ |
denotes |
developmental |
T8272 |
31370-31373 |
CC |
denotes |
and |
T8273 |
31374-31377 |
DT |
denotes |
the |
T8274 |
31383-31387 |
NN |
denotes |
type |
T8275 |
31378-31382 |
NN |
denotes |
cell |
T8276 |
31387-31388 |
. |
denotes |
. |
T8277 |
31388-31469 |
sentence |
denotes |
A role of Annexin A7 as well as of other annexins in nuclei remains speculative. |
T8278 |
31389-31390 |
DT |
denotes |
A |
T8279 |
31391-31395 |
NN |
denotes |
role |
T8280 |
31449-31456 |
VBZ |
denotes |
remains |
T8281 |
31396-31398 |
IN |
denotes |
of |
T8282 |
31399-31406 |
NN |
denotes |
Annexin |
T8283 |
31407-31409 |
NN |
denotes |
A7 |
T8284 |
31410-31412 |
RB |
denotes |
as |
T8285 |
31418-31420 |
IN |
denotes |
as |
T8286 |
31413-31417 |
RB |
denotes |
well |
T8287 |
31421-31423 |
IN |
denotes |
of |
T8288 |
31424-31429 |
JJ |
denotes |
other |
T8289 |
31430-31438 |
NNS |
denotes |
annexins |
T8290 |
31439-31441 |
IN |
denotes |
in |
T8291 |
31442-31448 |
NNS |
denotes |
nuclei |
T8292 |
31457-31468 |
JJ |
denotes |
speculative |
T8293 |
31468-31469 |
. |
denotes |
. |
T8294 |
31469-31601 |
sentence |
denotes |
The results obtained by immunofluorescence were confirmed by extraction of Annexin A7 from nuclei of neuronal and glial cell lines. |
T8295 |
31470-31473 |
DT |
denotes |
The |
T8296 |
31474-31481 |
NNS |
denotes |
results |
T8297 |
31518-31527 |
VBN |
denotes |
confirmed |
T8298 |
31482-31490 |
VBN |
denotes |
obtained |
T8299 |
31491-31493 |
IN |
denotes |
by |
T8300 |
31494-31512 |
NN |
denotes |
immunofluorescence |
T8301 |
31513-31517 |
VBD |
denotes |
were |
T8302 |
31528-31530 |
IN |
denotes |
by |
T8303 |
31531-31541 |
NN |
denotes |
extraction |
T8304 |
31542-31544 |
IN |
denotes |
of |
T8305 |
31545-31552 |
NN |
denotes |
Annexin |
T8306 |
31553-31555 |
NN |
denotes |
A7 |
T8307 |
31556-31560 |
IN |
denotes |
from |
T8308 |
31561-31567 |
NNS |
denotes |
nuclei |
T8309 |
31568-31570 |
IN |
denotes |
of |
T8310 |
31571-31579 |
JJ |
denotes |
neuronal |
T8311 |
31595-31600 |
NNS |
denotes |
lines |
T8312 |
31580-31583 |
CC |
denotes |
and |
T8313 |
31584-31589 |
JJ |
denotes |
glial |
T8314 |
31590-31594 |
NN |
denotes |
cell |
T8315 |
31600-31601 |
. |
denotes |
. |
T8507 |
31612-31619 |
NNS |
denotes |
Animals |
T8508 |
31620-31623 |
CC |
denotes |
and |
T8509 |
31624-31630 |
NN |
denotes |
tissue |
T8510 |
31631-31642 |
NN |
denotes |
preparation |
T8511 |
31642-31671 |
sentence |
denotes |
129SV strain mice were used. |
T8512 |
31643-31648 |
NN |
denotes |
129SV |
T8513 |
31649-31655 |
NN |
denotes |
strain |
T8514 |
31656-31660 |
NNS |
denotes |
mice |
T8515 |
31666-31670 |
VBN |
denotes |
used |
T8516 |
31661-31665 |
VBD |
denotes |
were |
T8517 |
31670-31671 |
. |
denotes |
. |
T8518 |
31671-31738 |
sentence |
denotes |
The day on which the vaginal plug was confirmed was defined as E0. |
T8519 |
31672-31675 |
DT |
denotes |
The |
T8520 |
31676-31679 |
NN |
denotes |
day |
T8521 |
31724-31731 |
VBN |
denotes |
defined |
T8522 |
31680-31682 |
IN |
denotes |
on |
T8523 |
31710-31719 |
VBN |
denotes |
confirmed |
T8524 |
31683-31688 |
WDT |
denotes |
which |
T8525 |
31689-31692 |
DT |
denotes |
the |
T8526 |
31701-31705 |
NN |
denotes |
plug |
T8527 |
31693-31700 |
JJ |
denotes |
vaginal |
T8528 |
31706-31709 |
VBD |
denotes |
was |
T8529 |
31720-31723 |
VBD |
denotes |
was |
T8530 |
31732-31734 |
IN |
denotes |
as |
T8531 |
31735-31737 |
NN |
denotes |
E0 |
T8532 |
31737-31738 |
. |
denotes |
. |
T8533 |
31738-31881 |
sentence |
denotes |
All the animal experiments were performed in accordance with the Guide for the Care and Use of Laboratory Animals (NIH Publication No. 85-23). |
T8534 |
31739-31742 |
PDT |
denotes |
All |
T8535 |
31754-31765 |
NNS |
denotes |
experiments |
T8536 |
31743-31746 |
DT |
denotes |
the |
T8537 |
31747-31753 |
NN |
denotes |
animal |
T8538 |
31771-31780 |
VBN |
denotes |
performed |
T8539 |
31766-31770 |
VBD |
denotes |
were |
T8540 |
31781-31783 |
IN |
denotes |
in |
T8541 |
31784-31794 |
NN |
denotes |
accordance |
T8542 |
31795-31799 |
IN |
denotes |
with |
T8543 |
31800-31803 |
DT |
denotes |
the |
T8544 |
31804-31809 |
NNP |
denotes |
Guide |
T8545 |
31810-31813 |
IN |
denotes |
for |
T8546 |
31814-31817 |
DT |
denotes |
the |
T8547 |
31818-31822 |
NNP |
denotes |
Care |
T8548 |
31823-31826 |
CC |
denotes |
and |
T8549 |
31827-31830 |
NNP |
denotes |
Use |
T8550 |
31831-31833 |
IN |
denotes |
of |
T8551 |
31834-31844 |
NNP |
denotes |
Laboratory |
T8552 |
31845-31852 |
NNPS |
denotes |
Animals |
T8553 |
31853-31854 |
-LRB- |
denotes |
( |
T8554 |
31854-31857 |
NNP |
denotes |
NIH |
T8555 |
31858-31869 |
NNP |
denotes |
Publication |
T8556 |
31870-31873 |
NN |
denotes |
No. |
T8557 |
31874-31876 |
CD |
denotes |
85 |
T8558 |
31876-31877 |
HYPH |
denotes |
- |
T8559 |
31877-31879 |
CD |
denotes |
23 |
T8560 |
31879-31880 |
-RRB- |
denotes |
) |
T8561 |
31880-31881 |
. |
denotes |
. |
T8562 |
31881-31979 |
sentence |
denotes |
Pregnant females were sacrificed by cervical dislocation at various intervals between E5 and E17. |
T8563 |
31882-31890 |
JJ |
denotes |
Pregnant |
T8564 |
31891-31898 |
NNS |
denotes |
females |
T8565 |
31904-31914 |
VBN |
denotes |
sacrificed |
T8566 |
31899-31903 |
VBD |
denotes |
were |
T8567 |
31915-31917 |
IN |
denotes |
by |
T8568 |
31918-31926 |
JJ |
denotes |
cervical |
T8569 |
31927-31938 |
NN |
denotes |
dislocation |
T8570 |
31939-31941 |
IN |
denotes |
at |
T8571 |
31942-31949 |
JJ |
denotes |
various |
T8572 |
31950-31959 |
NNS |
denotes |
intervals |
T8573 |
31960-31967 |
IN |
denotes |
between |
T8574 |
31968-31970 |
NN |
denotes |
E5 |
T8575 |
31971-31974 |
CC |
denotes |
and |
T8576 |
31975-31978 |
NN |
denotes |
E17 |
T8577 |
31978-31979 |
. |
denotes |
. |
T8578 |
31979-32040 |
sentence |
denotes |
Before E9, embryos were embedded within the maternal uterus. |
T8579 |
31980-31986 |
IN |
denotes |
Before |
T8580 |
32004-32012 |
VBN |
denotes |
embedded |
T8581 |
31987-31989 |
NN |
denotes |
E9 |
T8582 |
31989-31991 |
, |
denotes |
, |
T8583 |
31991-31998 |
NNS |
denotes |
embryos |
T8584 |
31999-32003 |
VBD |
denotes |
were |
T8585 |
32013-32019 |
IN |
denotes |
within |
T8586 |
32020-32023 |
DT |
denotes |
the |
T8587 |
32033-32039 |
NN |
denotes |
uterus |
T8588 |
32024-32032 |
JJ |
denotes |
maternal |
T8589 |
32039-32040 |
. |
denotes |
. |
T8590 |
32040-32117 |
sentence |
denotes |
After E9, embryos were removed from their membranes and embedded separately. |
T8591 |
32041-32046 |
IN |
denotes |
After |
T8592 |
32064-32071 |
VBN |
denotes |
removed |
T8593 |
32047-32049 |
NN |
denotes |
E9 |
T8594 |
32049-32051 |
, |
denotes |
, |
T8595 |
32051-32058 |
NNS |
denotes |
embryos |
T8596 |
32059-32063 |
VBD |
denotes |
were |
T8597 |
32072-32076 |
IN |
denotes |
from |
T8598 |
32077-32082 |
PRP$ |
denotes |
their |
T8599 |
32083-32092 |
NNS |
denotes |
membranes |
T8600 |
32093-32096 |
CC |
denotes |
and |
T8601 |
32097-32105 |
VBN |
denotes |
embedded |
T8602 |
32106-32116 |
RB |
denotes |
separately |
T8603 |
32116-32117 |
. |
denotes |
. |
T8604 |
32117-32198 |
sentence |
denotes |
Experiments were performed with four to six animals at each developmental stage. |
T8605 |
32118-32129 |
NNS |
denotes |
Experiments |
T8606 |
32135-32144 |
VBN |
denotes |
performed |
T8607 |
32130-32134 |
VBD |
denotes |
were |
T8608 |
32145-32149 |
IN |
denotes |
with |
T8609 |
32150-32154 |
CD |
denotes |
four |
T8610 |
32158-32161 |
CD |
denotes |
six |
T8611 |
32155-32157 |
IN |
denotes |
to |
T8612 |
32162-32169 |
NNS |
denotes |
animals |
T8613 |
32170-32172 |
IN |
denotes |
at |
T8614 |
32173-32177 |
DT |
denotes |
each |
T8615 |
32192-32197 |
NN |
denotes |
stage |
T8616 |
32178-32191 |
JJ |
denotes |
developmental |
T8617 |
32197-32198 |
. |
denotes |
. |
T8618 |
32198-32273 |
sentence |
denotes |
The mature brain used was derived from mice of three to four month of age. |
T8619 |
32199-32202 |
DT |
denotes |
The |
T8620 |
32210-32215 |
NN |
denotes |
brain |
T8621 |
32203-32209 |
JJ |
denotes |
mature |
T8622 |
32225-32232 |
VBN |
denotes |
derived |
T8623 |
32216-32220 |
VBN |
denotes |
used |
T8624 |
32221-32224 |
VBD |
denotes |
was |
T8625 |
32233-32237 |
IN |
denotes |
from |
T8626 |
32238-32242 |
NNS |
denotes |
mice |
T8627 |
32243-32245 |
IN |
denotes |
of |
T8628 |
32246-32251 |
CD |
denotes |
three |
T8629 |
32255-32259 |
CD |
denotes |
four |
T8630 |
32252-32254 |
IN |
denotes |
to |
T8631 |
32260-32265 |
NN |
denotes |
month |
T8632 |
32266-32268 |
IN |
denotes |
of |
T8633 |
32269-32272 |
NN |
denotes |
age |
T8634 |
32272-32273 |
. |
denotes |
. |
T8635 |
32273-32408 |
sentence |
denotes |
The embryos and tissues were fixed in freshly prepared 4% paraformaldehyde solution for 2–12 h depending on the tissue thickness [35]. |
T8636 |
32274-32277 |
DT |
denotes |
The |
T8637 |
32278-32285 |
NNS |
denotes |
embryos |
T8638 |
32303-32308 |
VBN |
denotes |
fixed |
T8639 |
32286-32289 |
CC |
denotes |
and |
T8640 |
32290-32297 |
NNS |
denotes |
tissues |
T8641 |
32298-32302 |
VBD |
denotes |
were |
T8642 |
32309-32311 |
IN |
denotes |
in |
T8643 |
32312-32319 |
RB |
denotes |
freshly |
T8644 |
32320-32328 |
VBN |
denotes |
prepared |
T8645 |
32349-32357 |
NN |
denotes |
solution |
T8646 |
32329-32330 |
CD |
denotes |
4 |
T8647 |
32330-32331 |
NN |
denotes |
% |
T8648 |
32332-32348 |
NN |
denotes |
paraformaldehyde |
T8649 |
32358-32361 |
IN |
denotes |
for |
T8650 |
32362-32363 |
CD |
denotes |
2 |
T8651 |
32364-32366 |
CD |
denotes |
12 |
T8652 |
32363-32364 |
SYM |
denotes |
– |
T8653 |
32367-32368 |
NN |
denotes |
h |
T8654 |
32369-32378 |
VBG |
denotes |
depending |
T8655 |
32379-32381 |
IN |
denotes |
on |
T8656 |
32382-32385 |
DT |
denotes |
the |
T8657 |
32393-32402 |
NN |
denotes |
thickness |
T8658 |
32386-32392 |
NN |
denotes |
tissue |
T8659 |
32403-32404 |
-LRB- |
denotes |
[ |
T8660 |
32404-32406 |
CD |
denotes |
35 |
T8661 |
32406-32407 |
-RRB- |
denotes |
] |
T8662 |
32407-32408 |
. |
denotes |
. |
T8663 |
32408-32501 |
sentence |
denotes |
They were dehydrated with ethanol and embedded in paraffin according to standard procedures. |
T8664 |
32409-32413 |
PRP |
denotes |
They |
T8665 |
32419-32429 |
VBN |
denotes |
dehydrated |
T8666 |
32414-32418 |
VBD |
denotes |
were |
T8667 |
32430-32434 |
IN |
denotes |
with |
T8668 |
32435-32442 |
NN |
denotes |
ethanol |
T8669 |
32443-32446 |
CC |
denotes |
and |
T8670 |
32447-32455 |
VBN |
denotes |
embedded |
T8671 |
32456-32458 |
IN |
denotes |
in |
T8672 |
32459-32467 |
NN |
denotes |
paraffin |
T8673 |
32468-32477 |
VBG |
denotes |
according |
T8674 |
32478-32480 |
IN |
denotes |
to |
T8675 |
32481-32489 |
JJ |
denotes |
standard |
T8676 |
32490-32500 |
NNS |
denotes |
procedures |
T8677 |
32500-32501 |
. |
denotes |
. |
T8678 |
32501-32595 |
sentence |
denotes |
Microtome sections were cut at 7–10 μm, placed on slides and stored until further processing. |
T8679 |
32502-32511 |
NN |
denotes |
Microtome |
T8680 |
32512-32520 |
NNS |
denotes |
sections |
T8681 |
32526-32529 |
VBN |
denotes |
cut |
T8682 |
32521-32525 |
VBD |
denotes |
were |
T8683 |
32530-32532 |
IN |
denotes |
at |
T8684 |
32533-32534 |
CD |
denotes |
7 |
T8685 |
32535-32537 |
CD |
denotes |
10 |
T8686 |
32534-32535 |
SYM |
denotes |
– |
T8687 |
32538-32540 |
NN |
denotes |
μm |
T8688 |
32540-32542 |
, |
denotes |
, |
T8689 |
32542-32548 |
VBN |
denotes |
placed |
T8690 |
32549-32551 |
IN |
denotes |
on |
T8691 |
32552-32558 |
NNS |
denotes |
slides |
T8692 |
32559-32562 |
CC |
denotes |
and |
T8693 |
32563-32569 |
VBN |
denotes |
stored |
T8694 |
32570-32575 |
IN |
denotes |
until |
T8695 |
32576-32583 |
JJ |
denotes |
further |
T8696 |
32584-32594 |
NN |
denotes |
processing |
T8697 |
32594-32595 |
. |
denotes |
. |
T8723 |
32597-32602 |
JJ |
denotes |
Human |
T8724 |
32609-32615 |
NN |
denotes |
tissue |
T8725 |
32603-32608 |
NN |
denotes |
brain |
T8726 |
32615-32823 |
sentence |
denotes |
Five human autopsy brains of male and female patients without any neuropathological changes (age 61–77 years; post mortem interval: 12–72 h) were fixed in an aqueous solution of formaldehyde for three weeks. |
T8727 |
32616-32620 |
CD |
denotes |
Five |
T8728 |
32635-32641 |
NNS |
denotes |
brains |
T8729 |
32621-32626 |
JJ |
denotes |
human |
T8730 |
32627-32634 |
NN |
denotes |
autopsy |
T8731 |
32762-32767 |
VBN |
denotes |
fixed |
T8732 |
32642-32644 |
IN |
denotes |
of |
T8733 |
32645-32649 |
JJ |
denotes |
male |
T8734 |
32661-32669 |
NNS |
denotes |
patients |
T8735 |
32650-32653 |
CC |
denotes |
and |
T8736 |
32654-32660 |
JJ |
denotes |
female |
T8737 |
32670-32677 |
IN |
denotes |
without |
T8738 |
32678-32681 |
DT |
denotes |
any |
T8739 |
32700-32707 |
NNS |
denotes |
changes |
T8740 |
32682-32699 |
JJ |
denotes |
neuropathological |
T8741 |
32708-32709 |
-LRB- |
denotes |
( |
T8742 |
32754-32755 |
NN |
denotes |
h |
T8743 |
32709-32712 |
NN |
denotes |
age |
T8744 |
32719-32724 |
NNS |
denotes |
years |
T8745 |
32713-32715 |
CD |
denotes |
61 |
T8746 |
32716-32718 |
CD |
denotes |
77 |
T8747 |
32715-32716 |
SYM |
denotes |
– |
T8748 |
32724-32725 |
: |
denotes |
; |
T8749 |
32726-32730 |
FW |
denotes |
post |
T8750 |
32731-32737 |
FW |
denotes |
mortem |
T8751 |
32738-32746 |
NN |
denotes |
interval |
T8752 |
32746-32748 |
: |
denotes |
: |
T8753 |
32748-32750 |
CD |
denotes |
12 |
T8754 |
32751-32753 |
CD |
denotes |
72 |
T8755 |
32750-32751 |
SYM |
denotes |
– |
T8756 |
32755-32756 |
-RRB- |
denotes |
) |
T8757 |
32757-32761 |
VBD |
denotes |
were |
T8758 |
32768-32770 |
IN |
denotes |
in |
T8759 |
32771-32773 |
DT |
denotes |
an |
T8760 |
32782-32790 |
NN |
denotes |
solution |
T8761 |
32774-32781 |
JJ |
denotes |
aqueous |
T8762 |
32791-32793 |
IN |
denotes |
of |
T8763 |
32794-32806 |
NN |
denotes |
formaldehyde |
T8764 |
32807-32810 |
IN |
denotes |
for |
T8765 |
32811-32816 |
CD |
denotes |
three |
T8766 |
32817-32822 |
NNS |
denotes |
weeks |
T8767 |
32822-32823 |
. |
denotes |
. |
T8768 |
32823-32917 |
sentence |
denotes |
Blocks of the parietal neocortex were embedded in paraffin and microtomed at 12 μm thickness. |
T8769 |
32824-32830 |
NNS |
denotes |
Blocks |
T8770 |
32862-32870 |
VBN |
denotes |
embedded |
T8771 |
32831-32833 |
IN |
denotes |
of |
T8772 |
32834-32837 |
DT |
denotes |
the |
T8773 |
32847-32856 |
NN |
denotes |
neocortex |
T8774 |
32838-32846 |
JJ |
denotes |
parietal |
T8775 |
32857-32861 |
VBD |
denotes |
were |
T8776 |
32871-32873 |
IN |
denotes |
in |
T8777 |
32874-32882 |
NN |
denotes |
paraffin |
T8778 |
32883-32886 |
CC |
denotes |
and |
T8779 |
32887-32897 |
VBN |
denotes |
microtomed |
T8780 |
32898-32900 |
IN |
denotes |
at |
T8781 |
32901-32903 |
CD |
denotes |
12 |
T8782 |
32904-32906 |
NN |
denotes |
μm |
T8783 |
32907-32916 |
NN |
denotes |
thickness |
T8784 |
32916-32917 |
. |
denotes |
. |
T8785 |
32917-33028 |
sentence |
denotes |
Sections were stained with aldehydefuchsin Darrow-red to confirm the absence of neuropathological alterations. |
T8786 |
32918-32926 |
NNS |
denotes |
Sections |
T8787 |
32932-32939 |
VBN |
denotes |
stained |
T8788 |
32927-32931 |
VBD |
denotes |
were |
T8789 |
32940-32944 |
IN |
denotes |
with |
T8790 |
32945-32960 |
NN |
denotes |
aldehydefuchsin |
T8791 |
32968-32971 |
NN |
denotes |
red |
T8792 |
32961-32967 |
NN |
denotes |
Darrow |
T8793 |
32967-32968 |
HYPH |
denotes |
- |
T8794 |
32972-32974 |
TO |
denotes |
to |
T8795 |
32975-32982 |
VB |
denotes |
confirm |
T8796 |
32983-32986 |
DT |
denotes |
the |
T8797 |
32987-32994 |
NN |
denotes |
absence |
T8798 |
32995-32997 |
IN |
denotes |
of |
T8799 |
32998-33015 |
JJ |
denotes |
neuropathological |
T8800 |
33016-33027 |
NNS |
denotes |
alterations |
T8801 |
33027-33028 |
. |
denotes |
. |
T8802 |
33028-33184 |
sentence |
denotes |
All procedures were conducted in accordance with the Declaration of Helsinki and approved by the ethics committee of the University of Bonn Medical Center. |
T8803 |
33029-33032 |
DT |
denotes |
All |
T8804 |
33033-33043 |
NNS |
denotes |
procedures |
T8805 |
33049-33058 |
VBN |
denotes |
conducted |
T8806 |
33044-33048 |
VBD |
denotes |
were |
T8807 |
33059-33061 |
IN |
denotes |
in |
T8808 |
33062-33072 |
NN |
denotes |
accordance |
T8809 |
33073-33077 |
IN |
denotes |
with |
T8810 |
33078-33081 |
DT |
denotes |
the |
T8811 |
33082-33093 |
NNP |
denotes |
Declaration |
T8812 |
33094-33096 |
IN |
denotes |
of |
T8813 |
33097-33105 |
NNP |
denotes |
Helsinki |
T8814 |
33106-33109 |
CC |
denotes |
and |
T8815 |
33110-33118 |
VBN |
denotes |
approved |
T8816 |
33119-33121 |
IN |
denotes |
by |
T8817 |
33122-33125 |
DT |
denotes |
the |
T8818 |
33133-33142 |
NN |
denotes |
committee |
T8819 |
33126-33132 |
NNS |
denotes |
ethics |
T8820 |
33143-33145 |
IN |
denotes |
of |
T8821 |
33146-33149 |
DT |
denotes |
the |
T8822 |
33150-33160 |
NNP |
denotes |
University |
T8823 |
33177-33183 |
NN |
denotes |
Center |
T8824 |
33161-33163 |
IN |
denotes |
of |
T8825 |
33164-33168 |
NNP |
denotes |
Bonn |
T8826 |
33169-33176 |
JJ |
denotes |
Medical |
T8827 |
33183-33184 |
. |
denotes |
. |
T8970 |
33186-33196 |
NNS |
denotes |
Antibodies |
T8971 |
33196-33735 |
sentence |
denotes |
Antibodies used in this study were mAb 203–217 directed against the core domain of mouse Annexin A7 [6] (purified IgG, 1:50) specifically recognizing both Annexin A7 isoforms [[6,10], and data not shown], a polyclonal antibody against mouse Annexin A7 [10] (1:200), a polyclonal antiserum raised against the astrocyte-specific intermediate filament glial fibrillary acidic protein (GFAP; DAKO, 1:400), anti-β-tubulin (Sigma, 1:1000), anti-Emerin (novocastra, 1:2000), and polyclonal anti-LAP2α (kindly provided by Ronald Foisner, 1:5000). |
T8972 |
33197-33207 |
NNS |
denotes |
Antibodies |
T8973 |
33227-33231 |
VBD |
denotes |
were |
T8974 |
33208-33212 |
VBN |
denotes |
used |
T8975 |
33213-33215 |
IN |
denotes |
in |
T8976 |
33216-33220 |
DT |
denotes |
this |
T8977 |
33221-33226 |
NN |
denotes |
study |
T8978 |
33232-33235 |
NN |
denotes |
mAb |
T8979 |
33236-33239 |
CD |
denotes |
203 |
T8980 |
33239-33240 |
SYM |
denotes |
– |
T8981 |
33240-33243 |
CD |
denotes |
217 |
T8982 |
33244-33252 |
VBN |
denotes |
directed |
T8983 |
33253-33260 |
IN |
denotes |
against |
T8984 |
33261-33264 |
DT |
denotes |
the |
T8985 |
33270-33276 |
NN |
denotes |
domain |
T8986 |
33265-33269 |
NN |
denotes |
core |
T8987 |
33277-33279 |
IN |
denotes |
of |
T8988 |
33280-33285 |
NN |
denotes |
mouse |
T8989 |
33294-33296 |
NN |
denotes |
A7 |
T8990 |
33286-33293 |
NN |
denotes |
Annexin |
T8991 |
33297-33298 |
-LRB- |
denotes |
[ |
T8992 |
33298-33299 |
CD |
denotes |
6 |
T8993 |
33299-33300 |
-RRB- |
denotes |
] |
T8994 |
33301-33302 |
-LRB- |
denotes |
( |
T8995 |
33316-33317 |
CD |
denotes |
1 |
T8996 |
33302-33310 |
VBN |
denotes |
purified |
T8997 |
33311-33314 |
NN |
denotes |
IgG |
T8998 |
33314-33316 |
, |
denotes |
, |
T8999 |
33317-33318 |
SYM |
denotes |
: |
T9000 |
33318-33320 |
CD |
denotes |
50 |
T9001 |
33320-33321 |
-RRB- |
denotes |
) |
T9002 |
33322-33334 |
RB |
denotes |
specifically |
T9003 |
33335-33346 |
VBG |
denotes |
recognizing |
T9004 |
33347-33351 |
DT |
denotes |
both |
T9005 |
33363-33371 |
NNS |
denotes |
isoforms |
T9006 |
33352-33359 |
NN |
denotes |
Annexin |
T9007 |
33360-33362 |
NN |
denotes |
A7 |
T9008 |
33372-33373 |
-LRB- |
denotes |
[ |
T9009 |
33376-33378 |
CD |
denotes |
10 |
T9010 |
33373-33374 |
-LRB- |
denotes |
[ |
T9011 |
33374-33375 |
CD |
denotes |
6 |
T9012 |
33375-33376 |
, |
denotes |
, |
T9013 |
33378-33379 |
-RRB- |
denotes |
] |
T9014 |
33379-33381 |
, |
denotes |
, |
T9015 |
33381-33384 |
CC |
denotes |
and |
T9016 |
33385-33389 |
NNS |
denotes |
data |
T9017 |
33394-33399 |
VBN |
denotes |
shown |
T9018 |
33390-33393 |
RB |
denotes |
not |
T9019 |
33399-33400 |
-RRB- |
denotes |
] |
T9020 |
33400-33402 |
, |
denotes |
, |
T9021 |
33402-33403 |
DT |
denotes |
a |
T9022 |
33415-33423 |
NN |
denotes |
antibody |
T9023 |
33404-33414 |
JJ |
denotes |
polyclonal |
T9024 |
33424-33431 |
IN |
denotes |
against |
T9025 |
33432-33437 |
NN |
denotes |
mouse |
T9026 |
33446-33448 |
NN |
denotes |
A7 |
T9027 |
33438-33445 |
NN |
denotes |
Annexin |
T9028 |
33449-33450 |
-LRB- |
denotes |
[ |
T9029 |
33450-33452 |
CD |
denotes |
10 |
T9030 |
33452-33453 |
-RRB- |
denotes |
] |
T9031 |
33454-33455 |
-LRB- |
denotes |
( |
T9032 |
33455-33456 |
CD |
denotes |
1 |
T9033 |
33456-33457 |
SYM |
denotes |
: |
T9034 |
33457-33460 |
CD |
denotes |
200 |
T9035 |
33460-33461 |
-RRB- |
denotes |
) |
T9036 |
33461-33463 |
, |
denotes |
, |
T9037 |
33463-33464 |
DT |
denotes |
a |
T9038 |
33476-33485 |
NN |
denotes |
antiserum |
T9039 |
33465-33475 |
JJ |
denotes |
polyclonal |
T9040 |
33486-33492 |
VBN |
denotes |
raised |
T9041 |
33493-33500 |
IN |
denotes |
against |
T9042 |
33501-33504 |
DT |
denotes |
the |
T9043 |
33570-33577 |
NN |
denotes |
protein |
T9044 |
33505-33514 |
NN |
denotes |
astrocyte |
T9045 |
33515-33523 |
JJ |
denotes |
specific |
T9046 |
33514-33515 |
HYPH |
denotes |
- |
T9047 |
33524-33536 |
JJ |
denotes |
intermediate |
T9048 |
33537-33545 |
NN |
denotes |
filament |
T9049 |
33546-33551 |
JJ |
denotes |
glial |
T9050 |
33552-33562 |
JJ |
denotes |
fibrillary |
T9051 |
33563-33569 |
JJ |
denotes |
acidic |
T9052 |
33578-33579 |
-LRB- |
denotes |
( |
T9053 |
33585-33589 |
NN |
denotes |
DAKO |
T9054 |
33579-33583 |
NN |
denotes |
GFAP |
T9055 |
33583-33584 |
: |
denotes |
; |
T9056 |
33589-33591 |
, |
denotes |
, |
T9057 |
33591-33592 |
CD |
denotes |
1 |
T9058 |
33592-33593 |
SYM |
denotes |
: |
T9059 |
33593-33596 |
CD |
denotes |
400 |
T9060 |
33596-33597 |
-RRB- |
denotes |
) |
T9061 |
33597-33599 |
, |
denotes |
, |
T9062 |
33599-33605 |
JJ |
denotes |
anti-β |
T9063 |
33606-33613 |
NN |
denotes |
tubulin |
T9064 |
33605-33606 |
HYPH |
denotes |
- |
T9065 |
33614-33615 |
-LRB- |
denotes |
( |
T9066 |
33622-33623 |
CD |
denotes |
1 |
T9067 |
33615-33620 |
NNP |
denotes |
Sigma |
T9068 |
33620-33622 |
, |
denotes |
, |
T9069 |
33623-33624 |
SYM |
denotes |
: |
T9070 |
33624-33628 |
CD |
denotes |
1000 |
T9071 |
33628-33629 |
-RRB- |
denotes |
) |
T9072 |
33629-33631 |
, |
denotes |
, |
T9073 |
33631-33642 |
NN |
denotes |
anti-Emerin |
T9074 |
33643-33644 |
-LRB- |
denotes |
( |
T9075 |
33656-33657 |
CD |
denotes |
1 |
T9076 |
33644-33654 |
NNP |
denotes |
novocastra |
T9077 |
33654-33656 |
, |
denotes |
, |
T9078 |
33657-33658 |
SYM |
denotes |
: |
T9079 |
33658-33662 |
CD |
denotes |
2000 |
T9080 |
33662-33663 |
-RRB- |
denotes |
) |
T9081 |
33663-33665 |
, |
denotes |
, |
T9082 |
33665-33668 |
CC |
denotes |
and |
T9083 |
33669-33679 |
JJ |
denotes |
polyclonal |
T9084 |
33680-33690 |
NN |
denotes |
anti-LAP2α |
T9085 |
33691-33692 |
-LRB- |
denotes |
( |
T9086 |
33692-33698 |
RB |
denotes |
kindly |
T9087 |
33699-33707 |
VBN |
denotes |
provided |
T9088 |
33708-33710 |
IN |
denotes |
by |
T9089 |
33711-33717 |
NNP |
denotes |
Ronald |
T9090 |
33718-33725 |
NNP |
denotes |
Foisner |
T9091 |
33725-33727 |
, |
denotes |
, |
T9092 |
33727-33728 |
CD |
denotes |
1 |
T9093 |
33728-33729 |
SYM |
denotes |
: |
T9094 |
33729-33733 |
CD |
denotes |
5000 |
T9095 |
33733-33734 |
-RRB- |
denotes |
) |
T9096 |
33734-33735 |
. |
denotes |
. |
T9606 |
33737-33755 |
NN |
denotes |
Immunofluorescence |
T9607 |
33756-33766 |
NN |
denotes |
microscopy |
T9608 |
33766-34000 |
sentence |
denotes |
Immunohistochemical staining using mAb 203–217 as the primary antibody was with AlexaFluor488-conjugated goat anti-mouse IgG (Molecular Probes, Leiden, The Nether-lands) or Cy3-labeled goat anti-mouse IgG as the secondary antibodies. |
T9609 |
33767-33786 |
JJ |
denotes |
Immunohistochemical |
T9610 |
33787-33795 |
NN |
denotes |
staining |
T9611 |
33838-33841 |
VBD |
denotes |
was |
T9612 |
33796-33801 |
VBG |
denotes |
using |
T9613 |
33802-33805 |
NN |
denotes |
mAb |
T9614 |
33806-33809 |
CD |
denotes |
203 |
T9615 |
33809-33810 |
SYM |
denotes |
– |
T9616 |
33810-33813 |
CD |
denotes |
217 |
T9617 |
33814-33816 |
IN |
denotes |
as |
T9618 |
33817-33820 |
DT |
denotes |
the |
T9619 |
33829-33837 |
NN |
denotes |
antibody |
T9620 |
33821-33828 |
JJ |
denotes |
primary |
T9621 |
33842-33846 |
IN |
denotes |
with |
T9622 |
33847-33860 |
NN |
denotes |
AlexaFluor488 |
T9623 |
33861-33871 |
VBN |
denotes |
conjugated |
T9624 |
33860-33861 |
HYPH |
denotes |
- |
T9625 |
33888-33891 |
NN |
denotes |
IgG |
T9626 |
33872-33876 |
NN |
denotes |
goat |
T9627 |
33877-33887 |
JJ |
denotes |
anti-mouse |
T9628 |
33892-33893 |
-LRB- |
denotes |
( |
T9629 |
33903-33909 |
NNP |
denotes |
Probes |
T9630 |
33893-33902 |
NNP |
denotes |
Molecular |
T9631 |
33909-33911 |
, |
denotes |
, |
T9632 |
33911-33917 |
NNP |
denotes |
Leiden |
T9633 |
33917-33919 |
, |
denotes |
, |
T9634 |
33919-33922 |
DT |
denotes |
The |
T9635 |
33930-33935 |
NNP |
denotes |
lands |
T9636 |
33923-33929 |
NNP |
denotes |
Nether |
T9637 |
33929-33930 |
HYPH |
denotes |
- |
T9638 |
33935-33936 |
-RRB- |
denotes |
) |
T9639 |
33937-33939 |
CC |
denotes |
or |
T9640 |
33940-33943 |
NN |
denotes |
Cy3 |
T9641 |
33944-33951 |
VBN |
denotes |
labeled |
T9642 |
33943-33944 |
HYPH |
denotes |
- |
T9643 |
33968-33971 |
NN |
denotes |
IgG |
T9644 |
33952-33956 |
NN |
denotes |
goat |
T9645 |
33957-33967 |
JJ |
denotes |
anti-mouse |
T9646 |
33972-33974 |
IN |
denotes |
as |
T9647 |
33975-33978 |
DT |
denotes |
the |
T9648 |
33989-33999 |
NNS |
denotes |
antibodies |
T9649 |
33979-33988 |
JJ |
denotes |
secondary |
T9650 |
33999-34000 |
. |
denotes |
. |
T9651 |
34000-34146 |
sentence |
denotes |
Polyclonal anti-Annexin A7 antibody and anti-GFAP-antibody were combined with Alexa Fluor 568-conjugated goat anti-rabbit IgG (Molecular Probes). |
T9652 |
34001-34011 |
JJ |
denotes |
Polyclonal |
T9653 |
34028-34036 |
NN |
denotes |
antibody |
T9654 |
34012-34024 |
JJ |
denotes |
anti-Annexin |
T9655 |
34025-34027 |
NN |
denotes |
A7 |
T9656 |
34065-34073 |
VBN |
denotes |
combined |
T9657 |
34037-34040 |
CC |
denotes |
and |
T9658 |
34041-34050 |
JJ |
denotes |
anti-GFAP |
T9659 |
34051-34059 |
NN |
denotes |
antibody |
T9660 |
34050-34051 |
HYPH |
denotes |
- |
T9661 |
34060-34064 |
VBD |
denotes |
were |
T9662 |
34074-34078 |
IN |
denotes |
with |
T9663 |
34079-34084 |
NNP |
denotes |
Alexa |
T9664 |
34085-34090 |
NNP |
denotes |
Fluor |
T9665 |
34095-34105 |
VBN |
denotes |
conjugated |
T9666 |
34091-34094 |
CD |
denotes |
568 |
T9667 |
34094-34095 |
HYPH |
denotes |
- |
T9668 |
34123-34126 |
NN |
denotes |
IgG |
T9669 |
34106-34110 |
NN |
denotes |
goat |
T9670 |
34111-34122 |
JJ |
denotes |
anti-rabbit |
T9671 |
34127-34128 |
-LRB- |
denotes |
( |
T9672 |
34138-34144 |
NNP |
denotes |
Probes |
T9673 |
34128-34137 |
NNP |
denotes |
Molecular |
T9674 |
34144-34145 |
-RRB- |
denotes |
) |
T9675 |
34145-34146 |
. |
denotes |
. |
T9676 |
34146-34349 |
sentence |
denotes |
The specific labelling of Annexin A7 detected with the anti-mouse-IgG secondary antibodies in mouse tissue sections was confirmed using primary antibodies, which were directly coupled to Alexa Fluor488. |
T9677 |
34147-34150 |
DT |
denotes |
The |
T9678 |
34160-34169 |
NN |
denotes |
labelling |
T9679 |
34151-34159 |
JJ |
denotes |
specific |
T9680 |
34267-34276 |
VBN |
denotes |
confirmed |
T9681 |
34170-34172 |
IN |
denotes |
of |
T9682 |
34173-34180 |
NN |
denotes |
Annexin |
T9683 |
34181-34183 |
NN |
denotes |
A7 |
T9684 |
34184-34192 |
VBN |
denotes |
detected |
T9685 |
34193-34197 |
IN |
denotes |
with |
T9686 |
34198-34201 |
DT |
denotes |
the |
T9687 |
34227-34237 |
NNS |
denotes |
antibodies |
T9688 |
34202-34212 |
JJ |
denotes |
anti-mouse |
T9689 |
34213-34216 |
NN |
denotes |
IgG |
T9690 |
34212-34213 |
HYPH |
denotes |
- |
T9691 |
34217-34226 |
JJ |
denotes |
secondary |
T9692 |
34238-34240 |
IN |
denotes |
in |
T9693 |
34241-34246 |
NN |
denotes |
mouse |
T9694 |
34254-34262 |
NNS |
denotes |
sections |
T9695 |
34247-34253 |
NN |
denotes |
tissue |
T9696 |
34263-34266 |
VBD |
denotes |
was |
T9697 |
34277-34282 |
VBG |
denotes |
using |
T9698 |
34283-34290 |
JJ |
denotes |
primary |
T9699 |
34291-34301 |
NNS |
denotes |
antibodies |
T9700 |
34301-34303 |
, |
denotes |
, |
T9701 |
34303-34308 |
WDT |
denotes |
which |
T9702 |
34323-34330 |
VBN |
denotes |
coupled |
T9703 |
34309-34313 |
VBD |
denotes |
were |
T9704 |
34314-34322 |
RB |
denotes |
directly |
T9705 |
34331-34333 |
IN |
denotes |
to |
T9706 |
34334-34339 |
NNP |
denotes |
Alexa |
T9707 |
34340-34348 |
NN |
denotes |
Fluor488 |
T9708 |
34348-34349 |
. |
denotes |
. |
T9709 |
34349-34553 |
sentence |
denotes |
The paraffin-embedded sections were first treated three times for 2 min in xylene to remove paraffin and then rehydrated step by step with descending concentrations of ethanol for antibody staining [35]. |
T9710 |
34350-34353 |
DT |
denotes |
The |
T9711 |
34372-34380 |
NNS |
denotes |
sections |
T9712 |
34354-34362 |
NN |
denotes |
paraffin |
T9713 |
34363-34371 |
VBN |
denotes |
embedded |
T9714 |
34362-34363 |
HYPH |
denotes |
- |
T9715 |
34392-34399 |
VBN |
denotes |
treated |
T9716 |
34381-34385 |
VBD |
denotes |
were |
T9717 |
34386-34391 |
RB |
denotes |
first |
T9718 |
34400-34405 |
CD |
denotes |
three |
T9719 |
34406-34411 |
NNS |
denotes |
times |
T9720 |
34412-34415 |
IN |
denotes |
for |
T9721 |
34416-34417 |
CD |
denotes |
2 |
T9722 |
34418-34421 |
NN |
denotes |
min |
T9723 |
34422-34424 |
IN |
denotes |
in |
T9724 |
34425-34431 |
NN |
denotes |
xylene |
T9725 |
34432-34434 |
TO |
denotes |
to |
T9726 |
34435-34441 |
VB |
denotes |
remove |
T9727 |
34442-34450 |
NN |
denotes |
paraffin |
T9728 |
34451-34454 |
CC |
denotes |
and |
T9729 |
34455-34459 |
RB |
denotes |
then |
T9730 |
34460-34470 |
VBD |
denotes |
rehydrated |
T9731 |
34471-34475 |
NN |
denotes |
step |
T9732 |
34476-34478 |
IN |
denotes |
by |
T9733 |
34479-34483 |
NN |
denotes |
step |
T9734 |
34484-34488 |
IN |
denotes |
with |
T9735 |
34489-34499 |
VBG |
denotes |
descending |
T9736 |
34500-34514 |
NNS |
denotes |
concentrations |
T9737 |
34515-34517 |
IN |
denotes |
of |
T9738 |
34518-34525 |
NN |
denotes |
ethanol |
T9739 |
34526-34529 |
IN |
denotes |
for |
T9740 |
34530-34538 |
NN |
denotes |
antibody |
T9741 |
34539-34547 |
NN |
denotes |
staining |
T9742 |
34548-34549 |
-LRB- |
denotes |
[ |
T9743 |
34549-34551 |
CD |
denotes |
35 |
T9744 |
34551-34552 |
-RRB- |
denotes |
] |
T9745 |
34552-34553 |
. |
denotes |
. |
T9746 |
34553-34795 |
sentence |
denotes |
After three washings in PBS (137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.4), the tissue sections were incubated in PBS containing 0.1% trypsin for 10 min at room temperature to free formaldehyde-crosslinked epitopes [15-17]. |
T9747 |
34554-34559 |
IN |
denotes |
After |
T9748 |
34673-34682 |
VBN |
denotes |
incubated |
T9749 |
34560-34565 |
CD |
denotes |
three |
T9750 |
34566-34574 |
NNS |
denotes |
washings |
T9751 |
34575-34577 |
IN |
denotes |
in |
T9752 |
34578-34581 |
NN |
denotes |
PBS |
T9753 |
34582-34583 |
-LRB- |
denotes |
( |
T9754 |
34590-34594 |
NN |
denotes |
NaCl |
T9755 |
34583-34586 |
CD |
denotes |
137 |
T9756 |
34587-34589 |
NN |
denotes |
mM |
T9757 |
34594-34596 |
, |
denotes |
, |
T9758 |
34596-34599 |
CD |
denotes |
2.7 |
T9759 |
34600-34602 |
NN |
denotes |
mM |
T9760 |
34603-34606 |
NN |
denotes |
KCl |
T9761 |
34606-34608 |
, |
denotes |
, |
T9762 |
34608-34611 |
CD |
denotes |
8.1 |
T9763 |
34612-34614 |
NN |
denotes |
mM |
T9764 |
34615-34622 |
NN |
denotes |
Na2HPO4 |
T9765 |
34622-34624 |
, |
denotes |
, |
T9766 |
34624-34627 |
CD |
denotes |
1.5 |
T9767 |
34628-34630 |
NN |
denotes |
mM |
T9768 |
34631-34637 |
NN |
denotes |
KH2PO4 |
T9769 |
34637-34639 |
, |
denotes |
, |
T9770 |
34639-34641 |
NN |
denotes |
pH |
T9771 |
34642-34645 |
CD |
denotes |
7.4 |
T9772 |
34645-34646 |
-RRB- |
denotes |
) |
T9773 |
34646-34648 |
, |
denotes |
, |
T9774 |
34648-34651 |
DT |
denotes |
the |
T9775 |
34659-34667 |
NNS |
denotes |
sections |
T9776 |
34652-34658 |
NN |
denotes |
tissue |
T9777 |
34668-34672 |
VBD |
denotes |
were |
T9778 |
34683-34685 |
IN |
denotes |
in |
T9779 |
34686-34689 |
NN |
denotes |
PBS |
T9780 |
34690-34700 |
VBG |
denotes |
containing |
T9781 |
34701-34704 |
CD |
denotes |
0.1 |
T9782 |
34704-34705 |
NN |
denotes |
% |
T9783 |
34706-34713 |
NN |
denotes |
trypsin |
T9784 |
34714-34717 |
IN |
denotes |
for |
T9785 |
34718-34720 |
CD |
denotes |
10 |
T9786 |
34721-34724 |
NN |
denotes |
min |
T9787 |
34725-34727 |
IN |
denotes |
at |
T9788 |
34728-34732 |
NN |
denotes |
room |
T9789 |
34733-34744 |
NN |
denotes |
temperature |
T9790 |
34745-34747 |
TO |
denotes |
to |
T9791 |
34748-34752 |
VB |
denotes |
free |
T9792 |
34753-34765 |
NN |
denotes |
formaldehyde |
T9793 |
34766-34777 |
VBN |
denotes |
crosslinked |
T9794 |
34765-34766 |
HYPH |
denotes |
- |
T9795 |
34778-34786 |
NNS |
denotes |
epitopes |
T9796 |
34787-34788 |
-LRB- |
denotes |
[ |
T9797 |
34788-34790 |
CD |
denotes |
15 |
T9798 |
34790-34791 |
SYM |
denotes |
- |
T9799 |
34791-34793 |
CD |
denotes |
17 |
T9800 |
34793-34794 |
-RRB- |
denotes |
] |
T9801 |
34794-34795 |
. |
denotes |
. |
T9802 |
34795-34991 |
sentence |
denotes |
After additional three washes with PBS, non-specific binding sites were blocked by incubating the slides with PBG (0.5 % BSA, 0.045 % fish gelatine in PBS) in 10% normal goat serum (NGS) for 2 h. |
T9803 |
34796-34801 |
IN |
denotes |
After |
T9804 |
34868-34875 |
VBN |
denotes |
blocked |
T9805 |
34802-34812 |
JJ |
denotes |
additional |
T9806 |
34819-34825 |
NNS |
denotes |
washes |
T9807 |
34813-34818 |
CD |
denotes |
three |
T9808 |
34826-34830 |
IN |
denotes |
with |
T9809 |
34831-34834 |
NN |
denotes |
PBS |
T9810 |
34834-34836 |
, |
denotes |
, |
T9811 |
34836-34848 |
JJ |
denotes |
non-specific |
T9812 |
34857-34862 |
NNS |
denotes |
sites |
T9813 |
34849-34856 |
NN |
denotes |
binding |
T9814 |
34863-34867 |
VBD |
denotes |
were |
T9815 |
34876-34878 |
IN |
denotes |
by |
T9816 |
34879-34889 |
VBG |
denotes |
incubating |
T9817 |
34890-34893 |
DT |
denotes |
the |
T9818 |
34894-34900 |
NNS |
denotes |
slides |
T9819 |
34901-34905 |
IN |
denotes |
with |
T9820 |
34906-34909 |
NN |
denotes |
PBG |
T9821 |
34910-34911 |
-LRB- |
denotes |
( |
T9822 |
34917-34920 |
NN |
denotes |
BSA |
T9823 |
34911-34914 |
CD |
denotes |
0.5 |
T9824 |
34915-34916 |
NN |
denotes |
% |
T9825 |
34920-34922 |
, |
denotes |
, |
T9826 |
34922-34927 |
CD |
denotes |
0.045 |
T9827 |
34928-34929 |
NN |
denotes |
% |
T9828 |
34935-34943 |
NN |
denotes |
gelatine |
T9829 |
34930-34934 |
NN |
denotes |
fish |
T9830 |
34944-34946 |
IN |
denotes |
in |
T9831 |
34947-34950 |
NN |
denotes |
PBS |
T9832 |
34950-34951 |
-RRB- |
denotes |
) |
T9833 |
34952-34954 |
IN |
denotes |
in |
T9834 |
34955-34957 |
CD |
denotes |
10 |
T9835 |
34957-34958 |
NN |
denotes |
% |
T9836 |
34959-34965 |
JJ |
denotes |
normal |
T9837 |
34971-34976 |
NN |
denotes |
serum |
T9838 |
34966-34970 |
NN |
denotes |
goat |
T9839 |
34977-34978 |
-LRB- |
denotes |
( |
T9840 |
34978-34981 |
NN |
denotes |
NGS |
T9841 |
34981-34982 |
-RRB- |
denotes |
) |
T9842 |
34983-34986 |
IN |
denotes |
for |
T9843 |
34987-34988 |
CD |
denotes |
2 |
T9844 |
34989-34990 |
NN |
denotes |
h |
T9845 |
34990-34991 |
. |
denotes |
. |
T9846 |
34991-35103 |
sentence |
denotes |
Incubation with the primary antibodies was performed at 4°C overnight diluted in PBG supplemented with 1 % NGS. |
T9847 |
34992-35002 |
NN |
denotes |
Incubation |
T9848 |
35062-35069 |
VBN |
denotes |
diluted |
T9849 |
35003-35007 |
IN |
denotes |
with |
T9850 |
35008-35011 |
DT |
denotes |
the |
T9851 |
35020-35030 |
NNS |
denotes |
antibodies |
T9852 |
35012-35019 |
JJ |
denotes |
primary |
T9853 |
35031-35034 |
VBD |
denotes |
was |
T9854 |
35035-35044 |
VBN |
denotes |
performed |
T9855 |
35045-35047 |
IN |
denotes |
at |
T9856 |
35048-35049 |
CD |
denotes |
4 |
T9857 |
35049-35051 |
NN |
denotes |
°C |
T9858 |
35052-35061 |
RB |
denotes |
overnight |
T9859 |
35070-35072 |
IN |
denotes |
in |
T9860 |
35073-35076 |
NN |
denotes |
PBG |
T9861 |
35077-35089 |
VBN |
denotes |
supplemented |
T9862 |
35090-35094 |
IN |
denotes |
with |
T9863 |
35095-35096 |
CD |
denotes |
1 |
T9864 |
35097-35098 |
NN |
denotes |
% |
T9865 |
35099-35102 |
NN |
denotes |
NGS |
T9866 |
35102-35103 |
. |
denotes |
. |
T9867 |
35103-35336 |
sentence |
denotes |
After five washes in PBG for 5 min each, the slides were incubated for 1 h with the secondary antibody at room temperature, washed three times in PBG and three times in PBS, 5 min each, rinsed in water and embedded in gelvatol [36]. |
T9868 |
35104-35109 |
IN |
denotes |
After |
T9869 |
35161-35170 |
VBN |
denotes |
incubated |
T9870 |
35110-35114 |
CD |
denotes |
five |
T9871 |
35115-35121 |
NNS |
denotes |
washes |
T9872 |
35122-35124 |
IN |
denotes |
in |
T9873 |
35125-35128 |
NN |
denotes |
PBG |
T9874 |
35129-35132 |
IN |
denotes |
for |
T9875 |
35133-35134 |
CD |
denotes |
5 |
T9876 |
35135-35138 |
NN |
denotes |
min |
T9877 |
35139-35143 |
DT |
denotes |
each |
T9878 |
35143-35145 |
, |
denotes |
, |
T9879 |
35145-35148 |
DT |
denotes |
the |
T9880 |
35149-35155 |
NNS |
denotes |
slides |
T9881 |
35156-35160 |
VBD |
denotes |
were |
T9882 |
35171-35174 |
IN |
denotes |
for |
T9883 |
35175-35176 |
CD |
denotes |
1 |
T9884 |
35177-35178 |
NN |
denotes |
h |
T9885 |
35179-35183 |
IN |
denotes |
with |
T9886 |
35184-35187 |
DT |
denotes |
the |
T9887 |
35198-35206 |
NN |
denotes |
antibody |
T9888 |
35188-35197 |
JJ |
denotes |
secondary |
T9889 |
35207-35209 |
IN |
denotes |
at |
T9890 |
35210-35214 |
NN |
denotes |
room |
T9891 |
35215-35226 |
NN |
denotes |
temperature |
T9892 |
35226-35228 |
, |
denotes |
, |
T9893 |
35228-35234 |
VBD |
denotes |
washed |
T9894 |
35235-35240 |
CD |
denotes |
three |
T9895 |
35241-35246 |
NNS |
denotes |
times |
T9896 |
35247-35249 |
IN |
denotes |
in |
T9897 |
35250-35253 |
NN |
denotes |
PBG |
T9898 |
35254-35257 |
CC |
denotes |
and |
T9899 |
35258-35263 |
CD |
denotes |
three |
T9900 |
35264-35269 |
NNS |
denotes |
times |
T9901 |
35270-35272 |
IN |
denotes |
in |
T9902 |
35273-35276 |
NN |
denotes |
PBS |
T9903 |
35276-35278 |
, |
denotes |
, |
T9904 |
35278-35279 |
CD |
denotes |
5 |
T9905 |
35280-35283 |
NNS |
denotes |
min |
T9906 |
35284-35288 |
DT |
denotes |
each |
T9907 |
35288-35290 |
, |
denotes |
, |
T9908 |
35290-35296 |
VBN |
denotes |
rinsed |
T9909 |
35297-35299 |
IN |
denotes |
in |
T9910 |
35300-35305 |
NN |
denotes |
water |
T9911 |
35306-35309 |
CC |
denotes |
and |
T9912 |
35310-35318 |
VBN |
denotes |
embedded |
T9913 |
35319-35321 |
IN |
denotes |
in |
T9914 |
35322-35330 |
NN |
denotes |
gelvatol |
T9915 |
35331-35332 |
-LRB- |
denotes |
[ |
T9916 |
35332-35334 |
CD |
denotes |
36 |
T9917 |
35334-35335 |
-RRB- |
denotes |
] |
T9918 |
35335-35336 |
. |
denotes |
. |
T9919 |
35336-35477 |
sentence |
denotes |
For control, sections were incubated only with the secondary antibody or sections obtained from an annexin A7 knockout mouse were used [10]. |
T9920 |
35337-35340 |
IN |
denotes |
For |
T9921 |
35364-35373 |
VBN |
denotes |
incubated |
T9922 |
35341-35348 |
NN |
denotes |
control |
T9923 |
35348-35350 |
, |
denotes |
, |
T9924 |
35350-35358 |
NNS |
denotes |
sections |
T9925 |
35359-35363 |
VBD |
denotes |
were |
T9926 |
35374-35378 |
RB |
denotes |
only |
T9927 |
35379-35383 |
IN |
denotes |
with |
T9928 |
35384-35387 |
DT |
denotes |
the |
T9929 |
35398-35406 |
NN |
denotes |
antibody |
T9930 |
35388-35397 |
JJ |
denotes |
secondary |
T9931 |
35407-35409 |
CC |
denotes |
or |
T9932 |
35410-35418 |
NNS |
denotes |
sections |
T9933 |
35467-35471 |
VBN |
denotes |
used |
T9934 |
35419-35427 |
VBN |
denotes |
obtained |
T9935 |
35428-35432 |
IN |
denotes |
from |
T9936 |
35433-35435 |
DT |
denotes |
an |
T9937 |
35456-35461 |
NN |
denotes |
mouse |
T9938 |
35436-35443 |
NN |
denotes |
annexin |
T9939 |
35444-35446 |
NN |
denotes |
A7 |
T9940 |
35447-35455 |
NN |
denotes |
knockout |
T9941 |
35462-35466 |
VBD |
denotes |
were |
T9942 |
35472-35473 |
-LRB- |
denotes |
[ |
T9943 |
35473-35475 |
CD |
denotes |
10 |
T9944 |
35475-35476 |
-RRB- |
denotes |
] |
T9945 |
35476-35477 |
. |
denotes |
. |
T9946 |
35477-35575 |
sentence |
denotes |
Samples were examined with a confocal laser scan microscope (Leica TCS SP; Leica, Bensheim, FRG). |
T9947 |
35478-35485 |
NNS |
denotes |
Samples |
T9948 |
35491-35499 |
VBN |
denotes |
examined |
T9949 |
35486-35490 |
VBD |
denotes |
were |
T9950 |
35500-35504 |
IN |
denotes |
with |
T9951 |
35505-35506 |
DT |
denotes |
a |
T9952 |
35527-35537 |
NN |
denotes |
microscope |
T9953 |
35507-35515 |
JJ |
denotes |
confocal |
T9954 |
35516-35521 |
NN |
denotes |
laser |
T9955 |
35522-35526 |
NN |
denotes |
scan |
T9956 |
35538-35539 |
-LRB- |
denotes |
( |
T9957 |
35549-35551 |
NN |
denotes |
SP |
T9958 |
35539-35544 |
NNP |
denotes |
Leica |
T9959 |
35545-35548 |
NN |
denotes |
TCS |
T9960 |
35551-35552 |
: |
denotes |
; |
T9961 |
35553-35558 |
NNP |
denotes |
Leica |
T9962 |
35558-35560 |
, |
denotes |
, |
T9963 |
35560-35568 |
NNP |
denotes |
Bensheim |
T9964 |
35568-35570 |
, |
denotes |
, |
T9965 |
35570-35573 |
NNP |
denotes |
FRG |
T9966 |
35573-35574 |
-RRB- |
denotes |
) |
T9967 |
35574-35575 |
. |
denotes |
. |
T10006 |
35577-35595 |
NN |
denotes |
Immunofluorescence |
T10007 |
35596-35606 |
NN |
denotes |
microscopy |
T10008 |
35607-35609 |
IN |
denotes |
of |
T10009 |
35610-35615 |
JJ |
denotes |
human |
T10010 |
35616-35621 |
NN |
denotes |
brain |
T10011 |
35621-35693 |
sentence |
denotes |
The paraffin-embedded human brain sections were deparaffinzed likewise. |
T10012 |
35622-35625 |
DT |
denotes |
The |
T10013 |
35656-35664 |
NNS |
denotes |
sections |
T10014 |
35626-35634 |
NN |
denotes |
paraffin |
T10015 |
35635-35643 |
VBN |
denotes |
embedded |
T10016 |
35634-35635 |
HYPH |
denotes |
- |
T10017 |
35644-35649 |
JJ |
denotes |
human |
T10018 |
35650-35655 |
NN |
denotes |
brain |
T10019 |
35670-35683 |
VBN |
denotes |
deparaffinzed |
T10020 |
35665-35669 |
VBD |
denotes |
were |
T10021 |
35684-35692 |
RB |
denotes |
likewise |
T10022 |
35692-35693 |
. |
denotes |
. |
T10023 |
35693-35780 |
sentence |
denotes |
Immunostaining for Annexin A7 was performed using the monoclonal antibody mAb 203–217. |
T10024 |
35694-35708 |
NN |
denotes |
Immunostaining |
T10025 |
35728-35737 |
VBN |
denotes |
performed |
T10026 |
35709-35712 |
IN |
denotes |
for |
T10027 |
35713-35720 |
NN |
denotes |
Annexin |
T10028 |
35721-35723 |
NN |
denotes |
A7 |
T10029 |
35724-35727 |
VBD |
denotes |
was |
T10030 |
35738-35743 |
VBG |
denotes |
using |
T10031 |
35744-35747 |
DT |
denotes |
the |
T10032 |
35759-35767 |
NN |
denotes |
antibody |
T10033 |
35748-35758 |
JJ |
denotes |
monoclonal |
T10034 |
35768-35771 |
NN |
denotes |
mAb |
T10035 |
35772-35775 |
CD |
denotes |
203 |
T10036 |
35775-35776 |
SYM |
denotes |
– |
T10037 |
35776-35779 |
CD |
denotes |
217 |
T10038 |
35779-35780 |
. |
denotes |
. |
T10039 |
35780-35836 |
sentence |
denotes |
Microwave pre-treatment was used for all sections [15]. |
T10040 |
35781-35790 |
NN |
denotes |
Microwave |
T10041 |
35791-35804 |
NN |
denotes |
pre-treatment |
T10042 |
35809-35813 |
VBN |
denotes |
used |
T10043 |
35805-35808 |
VBD |
denotes |
was |
T10044 |
35814-35817 |
IN |
denotes |
for |
T10045 |
35818-35821 |
DT |
denotes |
all |
T10046 |
35822-35830 |
NNS |
denotes |
sections |
T10047 |
35831-35832 |
-LRB- |
denotes |
[ |
T10048 |
35832-35834 |
CD |
denotes |
15 |
T10049 |
35834-35835 |
-RRB- |
denotes |
] |
T10050 |
35835-35836 |
. |
denotes |
. |
T10051 |
35836-35881 |
sentence |
denotes |
Trypsin pre-treatment was used as indicated. |
T10052 |
35837-35844 |
NN |
denotes |
Trypsin |
T10053 |
35845-35858 |
NN |
denotes |
pre-treatment |
T10054 |
35863-35867 |
VBN |
denotes |
used |
T10055 |
35859-35862 |
VBD |
denotes |
was |
T10056 |
35868-35870 |
IN |
denotes |
as |
T10057 |
35871-35880 |
VBN |
denotes |
indicated |
T10058 |
35880-35881 |
. |
denotes |
. |
T10059 |
35881-36053 |
sentence |
denotes |
Carbocyanine 2 and 3 labeled anti-mouse IgG secondary antibodies (Dianova, Hamburg, Germany) were used to detect the primary antibody and showed similar staining patterns. |
T10060 |
35882-35894 |
NN |
denotes |
Carbocyanine |
T10061 |
35895-35896 |
CD |
denotes |
2 |
T10062 |
35903-35910 |
VBN |
denotes |
labeled |
T10063 |
35897-35900 |
CC |
denotes |
and |
T10064 |
35901-35902 |
CD |
denotes |
3 |
T10065 |
35936-35946 |
NNS |
denotes |
antibodies |
T10066 |
35911-35921 |
JJ |
denotes |
anti-mouse |
T10067 |
35922-35925 |
NN |
denotes |
IgG |
T10068 |
35926-35935 |
JJ |
denotes |
secondary |
T10069 |
35980-35984 |
VBN |
denotes |
used |
T10070 |
35947-35948 |
-LRB- |
denotes |
( |
T10071 |
35948-35955 |
NNP |
denotes |
Dianova |
T10072 |
35955-35957 |
, |
denotes |
, |
T10073 |
35957-35964 |
NNP |
denotes |
Hamburg |
T10074 |
35964-35966 |
, |
denotes |
, |
T10075 |
35966-35973 |
NNP |
denotes |
Germany |
T10076 |
35973-35974 |
-RRB- |
denotes |
) |
T10077 |
35975-35979 |
VBD |
denotes |
were |
T10078 |
35985-35987 |
TO |
denotes |
to |
T10079 |
35988-35994 |
VB |
denotes |
detect |
T10080 |
35995-35998 |
DT |
denotes |
the |
T10081 |
36007-36015 |
NN |
denotes |
antibody |
T10082 |
35999-36006 |
JJ |
denotes |
primary |
T10083 |
36016-36019 |
CC |
denotes |
and |
T10084 |
36020-36026 |
VBD |
denotes |
showed |
T10085 |
36027-36034 |
JJ |
denotes |
similar |
T10086 |
36044-36052 |
NNS |
denotes |
patterns |
T10087 |
36035-36043 |
NN |
denotes |
staining |
T10088 |
36052-36053 |
. |
denotes |
. |
T10089 |
36053-36089 |
sentence |
denotes |
Blank controls were performed, too. |
T10090 |
36054-36059 |
JJ |
denotes |
Blank |
T10091 |
36060-36068 |
NNS |
denotes |
controls |
T10092 |
36074-36083 |
VBN |
denotes |
performed |
T10093 |
36069-36073 |
VBD |
denotes |
were |
T10094 |
36083-36085 |
, |
denotes |
, |
T10095 |
36085-36088 |
RB |
denotes |
too |
T10096 |
36088-36089 |
. |
denotes |
. |
T10126 |
36091-36095 |
NN |
denotes |
Cell |
T10127 |
36096-36103 |
NN |
denotes |
culture |
T10128 |
36115-36125 |
NNS |
denotes |
conditions |
T10129 |
36104-36107 |
CC |
denotes |
and |
T10130 |
36108-36114 |
NN |
denotes |
growth |
T10131 |
36125-36337 |
sentence |
denotes |
The cell lines used were cultured according to the instructions provided by ATCC: murine neuroblastoma neuro-2a (ATCC CCL-131), rat pheochromocytoma PC-12 (ATCC CRL-1721), and rat glioblastoma C6 (ATCC CCL-107). |
T10132 |
36126-36129 |
DT |
denotes |
The |
T10133 |
36135-36140 |
NNS |
denotes |
lines |
T10134 |
36130-36134 |
NN |
denotes |
cell |
T10135 |
36151-36159 |
VBN |
denotes |
cultured |
T10136 |
36141-36145 |
VBN |
denotes |
used |
T10137 |
36146-36150 |
VBD |
denotes |
were |
T10138 |
36160-36169 |
VBG |
denotes |
according |
T10139 |
36170-36172 |
IN |
denotes |
to |
T10140 |
36173-36176 |
DT |
denotes |
the |
T10141 |
36177-36189 |
NNS |
denotes |
instructions |
T10142 |
36190-36198 |
VBN |
denotes |
provided |
T10143 |
36199-36201 |
IN |
denotes |
by |
T10144 |
36202-36206 |
NN |
denotes |
ATCC |
T10145 |
36206-36208 |
: |
denotes |
: |
T10146 |
36208-36214 |
JJ |
denotes |
murine |
T10147 |
36229-36234 |
NN |
denotes |
neuro |
T10148 |
36215-36228 |
NN |
denotes |
neuroblastoma |
T10149 |
36234-36235 |
HYPH |
denotes |
- |
T10150 |
36235-36237 |
CD |
denotes |
2a |
T10151 |
36238-36239 |
-LRB- |
denotes |
( |
T10152 |
36244-36247 |
NN |
denotes |
CCL |
T10153 |
36239-36243 |
NN |
denotes |
ATCC |
T10154 |
36247-36248 |
HYPH |
denotes |
- |
T10155 |
36248-36251 |
CD |
denotes |
131 |
T10156 |
36251-36252 |
-RRB- |
denotes |
) |
T10157 |
36252-36254 |
, |
denotes |
, |
T10158 |
36254-36257 |
NN |
denotes |
rat |
T10159 |
36275-36277 |
NN |
denotes |
PC |
T10160 |
36258-36274 |
NN |
denotes |
pheochromocytoma |
T10161 |
36277-36278 |
HYPH |
denotes |
- |
T10162 |
36278-36280 |
CD |
denotes |
12 |
T10163 |
36281-36282 |
-LRB- |
denotes |
( |
T10164 |
36287-36290 |
NN |
denotes |
CRL |
T10165 |
36282-36286 |
NN |
denotes |
ATCC |
T10166 |
36290-36291 |
HYPH |
denotes |
- |
T10167 |
36291-36295 |
CD |
denotes |
1721 |
T10168 |
36295-36296 |
-RRB- |
denotes |
) |
T10169 |
36296-36298 |
, |
denotes |
, |
T10170 |
36298-36301 |
CC |
denotes |
and |
T10171 |
36302-36305 |
NN |
denotes |
rat |
T10172 |
36319-36321 |
NN |
denotes |
C6 |
T10173 |
36306-36318 |
NN |
denotes |
glioblastoma |
T10174 |
36322-36323 |
-LRB- |
denotes |
( |
T10175 |
36328-36331 |
NN |
denotes |
CCL |
T10176 |
36323-36327 |
NN |
denotes |
ATCC |
T10177 |
36331-36332 |
HYPH |
denotes |
- |
T10178 |
36332-36335 |
CD |
denotes |
107 |
T10179 |
36335-36336 |
-RRB- |
denotes |
) |
T10180 |
36336-36337 |
. |
denotes |
. |
T10181 |
36337-36463 |
sentence |
denotes |
One day before fixation with 4%paraformaldehyde and permeabilization with 0.5% Triton X-100, cells were seeded on coverslips. |
T10182 |
36338-36341 |
CD |
denotes |
One |
T10183 |
36342-36345 |
NN |
denotes |
day |
T10184 |
36346-36352 |
IN |
denotes |
before |
T10185 |
36442-36448 |
VBN |
denotes |
seeded |
T10186 |
36353-36361 |
NN |
denotes |
fixation |
T10187 |
36362-36366 |
IN |
denotes |
with |
T10188 |
36367-36368 |
CD |
denotes |
4 |
T10189 |
36368-36369 |
NN |
denotes |
% |
T10190 |
36369-36385 |
NN |
denotes |
paraformaldehyde |
T10191 |
36386-36389 |
CC |
denotes |
and |
T10192 |
36390-36406 |
NN |
denotes |
permeabilization |
T10193 |
36407-36411 |
IN |
denotes |
with |
T10194 |
36412-36415 |
CD |
denotes |
0.5 |
T10195 |
36415-36416 |
NN |
denotes |
% |
T10196 |
36424-36425 |
NN |
denotes |
X |
T10197 |
36417-36423 |
NN |
denotes |
Triton |
T10198 |
36425-36426 |
HYPH |
denotes |
- |
T10199 |
36426-36429 |
CD |
denotes |
100 |
T10200 |
36429-36431 |
, |
denotes |
, |
T10201 |
36431-36436 |
NNS |
denotes |
cells |
T10202 |
36437-36441 |
VBD |
denotes |
were |
T10203 |
36449-36451 |
IN |
denotes |
on |
T10204 |
36452-36462 |
NNS |
denotes |
coverslips |
T10205 |
36462-36463 |
. |
denotes |
. |
T10206 |
36463-36568 |
sentence |
denotes |
Immunostaining was performed as described for the tissue sections with or without the trypsin treatment. |
T10207 |
36464-36478 |
NN |
denotes |
Immunostaining |
T10208 |
36483-36492 |
VBN |
denotes |
performed |
T10209 |
36479-36482 |
VBD |
denotes |
was |
T10210 |
36493-36495 |
IN |
denotes |
as |
T10211 |
36496-36505 |
VBN |
denotes |
described |
T10212 |
36506-36509 |
IN |
denotes |
for |
T10213 |
36510-36513 |
DT |
denotes |
the |
T10214 |
36521-36529 |
NNS |
denotes |
sections |
T10215 |
36514-36520 |
NN |
denotes |
tissue |
T10216 |
36530-36534 |
IN |
denotes |
with |
T10217 |
36535-36537 |
CC |
denotes |
or |
T10218 |
36538-36545 |
IN |
denotes |
without |
T10219 |
36546-36549 |
DT |
denotes |
the |
T10220 |
36558-36567 |
NN |
denotes |
treatment |
T10221 |
36550-36557 |
NN |
denotes |
trypsin |
T10222 |
36567-36568 |
. |
denotes |
. |
T10539 |
36570-36580 |
NN |
denotes |
Extraction |
T10540 |
36581-36583 |
IN |
denotes |
of |
T10541 |
36584-36591 |
JJ |
denotes |
nuclear |
T10542 |
36600-36602 |
NN |
denotes |
A7 |
T10543 |
36592-36599 |
NN |
denotes |
Annexin |
T10544 |
36602-36667 |
sentence |
denotes |
Cultured cells were trypsinized, counted and washed once in PBS. |
T10545 |
36603-36611 |
VBN |
denotes |
Cultured |
T10546 |
36612-36617 |
NNS |
denotes |
cells |
T10547 |
36623-36634 |
VBN |
denotes |
trypsinized |
T10548 |
36618-36622 |
VBD |
denotes |
were |
T10549 |
36634-36636 |
, |
denotes |
, |
T10550 |
36636-36643 |
VBN |
denotes |
counted |
T10551 |
36644-36647 |
CC |
denotes |
and |
T10552 |
36648-36654 |
VBN |
denotes |
washed |
T10553 |
36655-36659 |
RB |
denotes |
once |
T10554 |
36660-36662 |
IN |
denotes |
in |
T10555 |
36663-36666 |
NN |
denotes |
PBS |
T10556 |
36666-36667 |
. |
denotes |
. |
T10557 |
36667-36924 |
sentence |
denotes |
They were resuspended in buffer1 (0.32 M sucrose, 10 mM Tris, pH8.0, 3 mM CaCl2, 2 mM Mg-acetate, 0.1 mM EDTA, 0.5% NP40, 1 mM DTT, 0.5 mM PMSF; 1 × 107cells/400μl buffer) and sonified on ice (sonifier UP200S, dr.hielscher, 40 s, amplitude 50%, cycle 0.5). |
T10558 |
36668-36672 |
PRP |
denotes |
They |
T10559 |
36678-36689 |
VBN |
denotes |
resuspended |
T10560 |
36673-36677 |
VBD |
denotes |
were |
T10561 |
36690-36692 |
IN |
denotes |
in |
T10562 |
36693-36700 |
NN |
denotes |
buffer1 |
T10563 |
36701-36702 |
-LRB- |
denotes |
( |
T10564 |
36709-36716 |
NN |
denotes |
sucrose |
T10565 |
36702-36706 |
CD |
denotes |
0.32 |
T10566 |
36707-36708 |
NN |
denotes |
M |
T10567 |
36716-36718 |
, |
denotes |
, |
T10568 |
36718-36720 |
CD |
denotes |
10 |
T10569 |
36721-36723 |
NN |
denotes |
mM |
T10570 |
36724-36728 |
NN |
denotes |
Tris |
T10571 |
36728-36730 |
, |
denotes |
, |
T10572 |
36730-36735 |
NN |
denotes |
pH8.0 |
T10573 |
36735-36737 |
, |
denotes |
, |
T10574 |
36737-36738 |
CD |
denotes |
3 |
T10575 |
36739-36741 |
NN |
denotes |
mM |
T10576 |
36742-36747 |
NN |
denotes |
CaCl2 |
T10577 |
36747-36749 |
, |
denotes |
, |
T10578 |
36749-36750 |
CD |
denotes |
2 |
T10579 |
36751-36753 |
NN |
denotes |
mM |
T10580 |
36757-36764 |
NN |
denotes |
acetate |
T10581 |
36754-36756 |
NN |
denotes |
Mg |
T10582 |
36756-36757 |
HYPH |
denotes |
- |
T10583 |
36764-36766 |
, |
denotes |
, |
T10584 |
36766-36769 |
CD |
denotes |
0.1 |
T10585 |
36770-36772 |
NN |
denotes |
mM |
T10586 |
36773-36777 |
NN |
denotes |
EDTA |
T10587 |
36777-36779 |
, |
denotes |
, |
T10588 |
36779-36782 |
CD |
denotes |
0.5 |
T10589 |
36782-36783 |
NN |
denotes |
% |
T10590 |
36784-36788 |
NN |
denotes |
NP40 |
T10591 |
36788-36790 |
, |
denotes |
, |
T10592 |
36790-36791 |
CD |
denotes |
1 |
T10593 |
36792-36794 |
NN |
denotes |
mM |
T10594 |
36795-36798 |
NN |
denotes |
DTT |
T10595 |
36798-36800 |
, |
denotes |
, |
T10596 |
36800-36803 |
CD |
denotes |
0.5 |
T10597 |
36804-36806 |
NN |
denotes |
mM |
T10598 |
36807-36811 |
NN |
denotes |
PMSF |
T10599 |
36811-36812 |
: |
denotes |
; |
T10600 |
36813-36814 |
CD |
denotes |
1 |
T10601 |
36817-36825 |
NNS |
denotes |
107cells |
T10602 |
36815-36816 |
SYM |
denotes |
× |
T10603 |
36825-36826 |
SYM |
denotes |
/ |
T10604 |
36832-36838 |
NN |
denotes |
buffer |
T10605 |
36826-36831 |
NN |
denotes |
400μl |
T10606 |
36838-36839 |
-RRB- |
denotes |
) |
T10607 |
36840-36843 |
CC |
denotes |
and |
T10608 |
36844-36852 |
VBN |
denotes |
sonified |
T10609 |
36853-36855 |
IN |
denotes |
on |
T10610 |
36856-36859 |
NN |
denotes |
ice |
T10611 |
36860-36861 |
-LRB- |
denotes |
( |
T10612 |
36913-36918 |
NN |
denotes |
cycle |
T10613 |
36861-36869 |
NN |
denotes |
sonifier |
T10614 |
36870-36876 |
NN |
denotes |
UP200S |
T10615 |
36876-36878 |
, |
denotes |
, |
T10616 |
36878-36890 |
NNP |
denotes |
dr.hielscher |
T10617 |
36890-36892 |
, |
denotes |
, |
T10618 |
36892-36894 |
CD |
denotes |
40 |
T10619 |
36895-36896 |
NN |
denotes |
s |
T10620 |
36896-36898 |
, |
denotes |
, |
T10621 |
36898-36907 |
NN |
denotes |
amplitude |
T10622 |
36910-36911 |
NN |
denotes |
% |
T10623 |
36908-36910 |
CD |
denotes |
50 |
T10624 |
36911-36913 |
, |
denotes |
, |
T10625 |
36919-36922 |
CD |
denotes |
0.5 |
T10626 |
36922-36923 |
-RRB- |
denotes |
) |
T10627 |
36923-36924 |
. |
denotes |
. |
T10628 |
36924-37009 |
sentence |
denotes |
This step was repeated until no more intact cells were observed by light microscopy. |
T10629 |
36925-36929 |
DT |
denotes |
This |
T10630 |
36930-36934 |
NN |
denotes |
step |
T10631 |
36939-36947 |
VBN |
denotes |
repeated |
T10632 |
36935-36938 |
VBD |
denotes |
was |
T10633 |
36948-36953 |
IN |
denotes |
until |
T10634 |
36980-36988 |
VBN |
denotes |
observed |
T10635 |
36954-36956 |
DT |
denotes |
no |
T10636 |
36969-36974 |
NNS |
denotes |
cells |
T10637 |
36957-36961 |
JJR |
denotes |
more |
T10638 |
36962-36968 |
JJ |
denotes |
intact |
T10639 |
36975-36979 |
VBD |
denotes |
were |
T10640 |
36989-36991 |
IN |
denotes |
by |
T10641 |
36992-36997 |
NN |
denotes |
light |
T10642 |
36998-37008 |
NN |
denotes |
microscopy |
T10643 |
37008-37009 |
. |
denotes |
. |
T10644 |
37009-37140 |
sentence |
denotes |
The resulting homogenate was centrifuged at 500 g for 5 minutes, washed twice in 1 ml buffer 1 without NP40 and centrifuged again. |
T10645 |
37010-37013 |
DT |
denotes |
The |
T10646 |
37024-37034 |
NN |
denotes |
homogenate |
T10647 |
37014-37023 |
VBG |
denotes |
resulting |
T10648 |
37039-37050 |
VBN |
denotes |
centrifuged |
T10649 |
37035-37038 |
VBD |
denotes |
was |
T10650 |
37051-37053 |
IN |
denotes |
at |
T10651 |
37054-37057 |
CD |
denotes |
500 |
T10652 |
37058-37059 |
NN |
denotes |
g |
T10653 |
37060-37063 |
IN |
denotes |
for |
T10654 |
37064-37065 |
CD |
denotes |
5 |
T10655 |
37066-37073 |
NNS |
denotes |
minutes |
T10656 |
37073-37075 |
, |
denotes |
, |
T10657 |
37075-37081 |
VBN |
denotes |
washed |
T10658 |
37082-37087 |
RB |
denotes |
twice |
T10659 |
37088-37090 |
IN |
denotes |
in |
T10660 |
37091-37092 |
CD |
denotes |
1 |
T10661 |
37093-37095 |
NN |
denotes |
ml |
T10662 |
37096-37102 |
NN |
denotes |
buffer |
T10663 |
37103-37104 |
CD |
denotes |
1 |
T10664 |
37105-37112 |
IN |
denotes |
without |
T10665 |
37113-37117 |
NN |
denotes |
NP40 |
T10666 |
37118-37121 |
CC |
denotes |
and |
T10667 |
37122-37133 |
VBN |
denotes |
centrifuged |
T10668 |
37134-37139 |
RB |
denotes |
again |
T10669 |
37139-37140 |
. |
denotes |
. |
T10670 |
37140-37187 |
sentence |
denotes |
The resulting pellet contains purified nuclei. |
T10671 |
37141-37144 |
DT |
denotes |
The |
T10672 |
37155-37161 |
NN |
denotes |
pellet |
T10673 |
37145-37154 |
VBG |
denotes |
resulting |
T10674 |
37162-37170 |
VBZ |
denotes |
contains |
T10675 |
37171-37179 |
VBN |
denotes |
purified |
T10676 |
37180-37186 |
NNS |
denotes |
nuclei |
T10677 |
37186-37187 |
. |
denotes |
. |
T10678 |
37187-37457 |
sentence |
denotes |
For partial extraction of the nucleoplasm according to [37], nuclei were resuspended in buffer 2 (20 mM Hepes, pH 7.9, 1.5 mM MgCl2, 20 mM KCl, 0.2 mM EDTA, 25%v/v glycerine, 0.5 mM DTT, 0.5 mM PMSF; nuclei of 107cells/30 μl buffer) and incubated on ice for 30 minutes. |
T10679 |
37188-37191 |
IN |
denotes |
For |
T10680 |
37261-37272 |
VBN |
denotes |
resuspended |
T10681 |
37192-37199 |
JJ |
denotes |
partial |
T10682 |
37200-37210 |
NN |
denotes |
extraction |
T10683 |
37211-37213 |
IN |
denotes |
of |
T10684 |
37214-37217 |
DT |
denotes |
the |
T10685 |
37218-37229 |
NN |
denotes |
nucleoplasm |
T10686 |
37230-37239 |
VBG |
denotes |
according |
T10687 |
37240-37242 |
IN |
denotes |
to |
T10688 |
37243-37244 |
-LRB- |
denotes |
[ |
T10689 |
37244-37246 |
CD |
denotes |
37 |
T10690 |
37246-37247 |
-RRB- |
denotes |
] |
T10691 |
37247-37249 |
, |
denotes |
, |
T10692 |
37249-37255 |
NNS |
denotes |
nuclei |
T10693 |
37256-37260 |
VBD |
denotes |
were |
T10694 |
37273-37275 |
IN |
denotes |
in |
T10695 |
37276-37282 |
NN |
denotes |
buffer |
T10696 |
37283-37284 |
CD |
denotes |
2 |
T10697 |
37285-37286 |
-LRB- |
denotes |
( |
T10698 |
37292-37297 |
NN |
denotes |
Hepes |
T10699 |
37286-37288 |
CD |
denotes |
20 |
T10700 |
37289-37291 |
NN |
denotes |
mM |
T10701 |
37297-37299 |
, |
denotes |
, |
T10702 |
37299-37301 |
NN |
denotes |
pH |
T10703 |
37302-37305 |
CD |
denotes |
7.9 |
T10704 |
37305-37307 |
, |
denotes |
, |
T10705 |
37307-37310 |
CD |
denotes |
1.5 |
T10706 |
37311-37313 |
NN |
denotes |
mM |
T10707 |
37314-37319 |
NN |
denotes |
MgCl2 |
T10708 |
37319-37321 |
, |
denotes |
, |
T10709 |
37321-37323 |
CD |
denotes |
20 |
T10710 |
37324-37326 |
NN |
denotes |
mM |
T10711 |
37327-37330 |
NN |
denotes |
KCl |
T10712 |
37330-37332 |
, |
denotes |
, |
T10713 |
37332-37335 |
CD |
denotes |
0.2 |
T10714 |
37336-37338 |
NN |
denotes |
mM |
T10715 |
37339-37343 |
NN |
denotes |
EDTA |
T10716 |
37343-37345 |
, |
denotes |
, |
T10717 |
37345-37347 |
CD |
denotes |
25 |
T10718 |
37347-37348 |
NN |
denotes |
% |
T10719 |
37352-37361 |
NN |
denotes |
glycerine |
T10720 |
37348-37349 |
NN |
denotes |
v |
T10721 |
37349-37350 |
SYM |
denotes |
/ |
T10722 |
37350-37351 |
NN |
denotes |
v |
T10723 |
37361-37363 |
, |
denotes |
, |
T10724 |
37363-37366 |
CD |
denotes |
0.5 |
T10725 |
37367-37369 |
NN |
denotes |
mM |
T10726 |
37370-37373 |
NN |
denotes |
DTT |
T10727 |
37373-37375 |
, |
denotes |
, |
T10728 |
37375-37378 |
CD |
denotes |
0.5 |
T10729 |
37379-37381 |
NN |
denotes |
mM |
T10730 |
37382-37386 |
NN |
denotes |
PMSF |
T10731 |
37386-37387 |
: |
denotes |
; |
T10732 |
37388-37394 |
NNS |
denotes |
nuclei |
T10733 |
37395-37397 |
IN |
denotes |
of |
T10734 |
37398-37406 |
NNS |
denotes |
107cells |
T10735 |
37413-37419 |
NN |
denotes |
buffer |
T10736 |
37406-37407 |
SYM |
denotes |
/ |
T10737 |
37410-37412 |
NN |
denotes |
μl |
T10738 |
37407-37409 |
CD |
denotes |
30 |
T10739 |
37419-37420 |
-RRB- |
denotes |
) |
T10740 |
37421-37424 |
CC |
denotes |
and |
T10741 |
37425-37434 |
VBN |
denotes |
incubated |
T10742 |
37435-37437 |
IN |
denotes |
on |
T10743 |
37438-37441 |
NN |
denotes |
ice |
T10744 |
37442-37445 |
IN |
denotes |
for |
T10745 |
37446-37448 |
CD |
denotes |
30 |
T10746 |
37449-37456 |
NNS |
denotes |
minutes |
T10747 |
37456-37457 |
. |
denotes |
. |
T10748 |
37457-37733 |
sentence |
denotes |
An equal volume of buffer 3 was added in aliquots (20 mM Hepes, pH 7.9, 1.5 mM MgCl2, 800 mM KCl, 0.2 mM EDTA, 25%v/v glycerine, 0.5 mM DTT, 0.5 mM PMSF, 1% NP40, protease inhibitor cocktail (Sigma)) and the solution was incubated while shaking for further 30 minutes on ice. |
T10749 |
37458-37460 |
DT |
denotes |
An |
T10750 |
37467-37473 |
NN |
denotes |
volume |
T10751 |
37461-37466 |
JJ |
denotes |
equal |
T10752 |
37490-37495 |
VBN |
denotes |
added |
T10753 |
37474-37476 |
IN |
denotes |
of |
T10754 |
37477-37483 |
NN |
denotes |
buffer |
T10755 |
37484-37485 |
CD |
denotes |
3 |
T10756 |
37486-37489 |
VBD |
denotes |
was |
T10757 |
37496-37498 |
IN |
denotes |
in |
T10758 |
37499-37507 |
NNS |
denotes |
aliquots |
T10759 |
37508-37509 |
-LRB- |
denotes |
( |
T10760 |
37650-37655 |
NNP |
denotes |
Sigma |
T10761 |
37509-37511 |
CD |
denotes |
20 |
T10762 |
37512-37514 |
NN |
denotes |
mM |
T10763 |
37515-37520 |
NN |
denotes |
Hepes |
T10764 |
37520-37522 |
, |
denotes |
, |
T10765 |
37522-37524 |
NN |
denotes |
pH |
T10766 |
37525-37528 |
CD |
denotes |
7.9 |
T10767 |
37528-37530 |
, |
denotes |
, |
T10768 |
37530-37533 |
CD |
denotes |
1.5 |
T10769 |
37534-37536 |
NN |
denotes |
mM |
T10770 |
37537-37542 |
NN |
denotes |
MgCl2 |
T10771 |
37542-37544 |
, |
denotes |
, |
T10772 |
37544-37547 |
CD |
denotes |
800 |
T10773 |
37548-37550 |
NN |
denotes |
mM |
T10774 |
37551-37554 |
NN |
denotes |
KCl |
T10775 |
37554-37556 |
, |
denotes |
, |
T10776 |
37556-37559 |
CD |
denotes |
0.2 |
T10777 |
37560-37562 |
NN |
denotes |
mM |
T10778 |
37563-37567 |
NN |
denotes |
EDTA |
T10779 |
37567-37569 |
, |
denotes |
, |
T10780 |
37569-37571 |
CD |
denotes |
25 |
T10781 |
37571-37572 |
NN |
denotes |
% |
T10782 |
37576-37585 |
NN |
denotes |
glycerine |
T10783 |
37572-37573 |
NN |
denotes |
v |
T10784 |
37573-37574 |
SYM |
denotes |
/ |
T10785 |
37574-37575 |
NN |
denotes |
v |
T10786 |
37585-37587 |
, |
denotes |
, |
T10787 |
37587-37590 |
CD |
denotes |
0.5 |
T10788 |
37591-37593 |
NN |
denotes |
mM |
T10789 |
37594-37597 |
NN |
denotes |
DTT |
T10790 |
37597-37599 |
, |
denotes |
, |
T10791 |
37599-37602 |
CD |
denotes |
0.5 |
T10792 |
37603-37605 |
NN |
denotes |
mM |
T10793 |
37606-37610 |
NN |
denotes |
PMSF |
T10794 |
37610-37612 |
, |
denotes |
, |
T10795 |
37612-37613 |
CD |
denotes |
1 |
T10796 |
37613-37614 |
NN |
denotes |
% |
T10797 |
37615-37619 |
NN |
denotes |
NP40 |
T10798 |
37619-37621 |
, |
denotes |
, |
T10799 |
37621-37629 |
NN |
denotes |
protease |
T10800 |
37630-37639 |
NN |
denotes |
inhibitor |
T10801 |
37640-37648 |
NN |
denotes |
cocktail |
T10802 |
37649-37650 |
-LRB- |
denotes |
( |
T10803 |
37655-37656 |
-RRB- |
denotes |
) |
T10804 |
37656-37657 |
-RRB- |
denotes |
) |
T10805 |
37658-37661 |
CC |
denotes |
and |
T10806 |
37662-37665 |
DT |
denotes |
the |
T10807 |
37666-37674 |
NN |
denotes |
solution |
T10808 |
37679-37688 |
VBN |
denotes |
incubated |
T10809 |
37675-37678 |
VBD |
denotes |
was |
T10810 |
37689-37694 |
IN |
denotes |
while |
T10811 |
37695-37702 |
VBG |
denotes |
shaking |
T10812 |
37703-37706 |
IN |
denotes |
for |
T10813 |
37707-37714 |
JJ |
denotes |
further |
T10814 |
37718-37725 |
NNS |
denotes |
minutes |
T10815 |
37715-37717 |
CD |
denotes |
30 |
T10816 |
37726-37728 |
IN |
denotes |
on |
T10817 |
37729-37732 |
NN |
denotes |
ice |
T10818 |
37732-37733 |
. |
denotes |
. |
T10819 |
37733-37919 |
sentence |
denotes |
Then the solution was centrifuged for 15 minutes at 14.000 g, the resulting supernatant was used as nucleoplasm, and the pellet was washed once and adjusted to an equal volume with PBS. |
T10820 |
37734-37738 |
RB |
denotes |
Then |
T10821 |
37756-37767 |
VBN |
denotes |
centrifuged |
T10822 |
37739-37742 |
DT |
denotes |
the |
T10823 |
37743-37751 |
NN |
denotes |
solution |
T10824 |
37752-37755 |
VBD |
denotes |
was |
T10825 |
37768-37771 |
IN |
denotes |
for |
T10826 |
37772-37774 |
CD |
denotes |
15 |
T10827 |
37775-37782 |
NNS |
denotes |
minutes |
T10828 |
37783-37785 |
IN |
denotes |
at |
T10829 |
37786-37792 |
CD |
denotes |
14.000 |
T10830 |
37793-37794 |
NN |
denotes |
g |
T10831 |
37794-37796 |
, |
denotes |
, |
T10832 |
37796-37799 |
DT |
denotes |
the |
T10833 |
37810-37821 |
NN |
denotes |
supernatant |
T10834 |
37800-37809 |
VBG |
denotes |
resulting |
T10835 |
37826-37830 |
VBN |
denotes |
used |
T10836 |
37822-37825 |
VBD |
denotes |
was |
T10837 |
37831-37833 |
IN |
denotes |
as |
T10838 |
37834-37845 |
NN |
denotes |
nucleoplasm |
T10839 |
37845-37847 |
, |
denotes |
, |
T10840 |
37847-37850 |
CC |
denotes |
and |
T10841 |
37851-37854 |
DT |
denotes |
the |
T10842 |
37855-37861 |
NN |
denotes |
pellet |
T10843 |
37866-37872 |
VBN |
denotes |
washed |
T10844 |
37862-37865 |
VBD |
denotes |
was |
T10845 |
37873-37877 |
RB |
denotes |
once |
T10846 |
37878-37881 |
CC |
denotes |
and |
T10847 |
37882-37890 |
VBN |
denotes |
adjusted |
T10848 |
37891-37893 |
IN |
denotes |
to |
T10849 |
37894-37896 |
DT |
denotes |
an |
T10850 |
37903-37909 |
NN |
denotes |
volume |
T10851 |
37897-37902 |
JJ |
denotes |
equal |
T10852 |
37910-37914 |
IN |
denotes |
with |
T10853 |
37915-37918 |
NN |
denotes |
PBS |
T10854 |
37918-37919 |
. |
denotes |
. |
T10855 |
37919-37999 |
sentence |
denotes |
Nucleoplasm and nuclear pellet were subjected to SDS-PAGE and Western blotting. |
T10856 |
37920-37931 |
NN |
denotes |
Nucleoplasm |
T10857 |
37956-37965 |
VBN |
denotes |
subjected |
T10858 |
37932-37935 |
CC |
denotes |
and |
T10859 |
37936-37943 |
JJ |
denotes |
nuclear |
T10860 |
37944-37950 |
NN |
denotes |
pellet |
T10861 |
37951-37955 |
VBD |
denotes |
were |
T10862 |
37966-37968 |
IN |
denotes |
to |
T10863 |
37969-37972 |
NN |
denotes |
SDS |
T10864 |
37973-37977 |
NN |
denotes |
PAGE |
T10865 |
37972-37973 |
HYPH |
denotes |
- |
T10866 |
37978-37981 |
CC |
denotes |
and |
T10867 |
37982-37989 |
NNP |
denotes |
Western |
T10868 |
37990-37998 |
NN |
denotes |
blotting |
T10869 |
37998-37999 |
. |
denotes |
. |
T10896 |
38001-38008 |
NNP |
denotes |
Western |
T10897 |
38009-38017 |
NN |
denotes |
blotting |
T10898 |
38017-38121 |
sentence |
denotes |
Cells or cellular fractions were lysed in SDS sample buffer and homogenates were subjected to SDS-PAGE. |
T10899 |
38018-38023 |
NNS |
denotes |
Cells |
T10900 |
38051-38056 |
VBN |
denotes |
lysed |
T10901 |
38024-38026 |
CC |
denotes |
or |
T10902 |
38027-38035 |
JJ |
denotes |
cellular |
T10903 |
38036-38045 |
NNS |
denotes |
fractions |
T10904 |
38046-38050 |
VBD |
denotes |
were |
T10905 |
38057-38059 |
IN |
denotes |
in |
T10906 |
38060-38063 |
NN |
denotes |
SDS |
T10907 |
38071-38077 |
NN |
denotes |
buffer |
T10908 |
38064-38070 |
NN |
denotes |
sample |
T10909 |
38078-38081 |
CC |
denotes |
and |
T10910 |
38082-38093 |
NNS |
denotes |
homogenates |
T10911 |
38099-38108 |
VBN |
denotes |
subjected |
T10912 |
38094-38098 |
VBD |
denotes |
were |
T10913 |
38109-38111 |
IN |
denotes |
to |
T10914 |
38112-38115 |
NN |
denotes |
SDS |
T10915 |
38116-38120 |
NN |
denotes |
PAGE |
T10916 |
38115-38116 |
HYPH |
denotes |
- |
T10917 |
38120-38121 |
. |
denotes |
. |
T10918 |
38121-38194 |
sentence |
denotes |
Western blotting was carried out using the method of Towbin et al. [38]. |
T10919 |
38122-38129 |
NNP |
denotes |
Western |
T10920 |
38130-38138 |
NN |
denotes |
blotting |
T10921 |
38143-38150 |
VBN |
denotes |
carried |
T10922 |
38139-38142 |
VBD |
denotes |
was |
T10923 |
38151-38154 |
RP |
denotes |
out |
T10924 |
38155-38160 |
VBG |
denotes |
using |
T10925 |
38161-38164 |
DT |
denotes |
the |
T10926 |
38165-38171 |
NN |
denotes |
method |
T10927 |
38172-38174 |
IN |
denotes |
of |
T10928 |
38175-38181 |
NNP |
denotes |
Towbin |
T10929 |
38182-38184 |
FW |
denotes |
et |
T10930 |
38185-38188 |
FW |
denotes |
al. |
T10931 |
38189-38190 |
-LRB- |
denotes |
[ |
T10932 |
38190-38192 |
CD |
denotes |
38 |
T10933 |
38192-38193 |
-RRB- |
denotes |
] |
T10934 |
38193-38194 |
. |
denotes |
. |
T10935 |
38194-38335 |
sentence |
denotes |
Peroxidase-coupled secondary antibodies (Sigma) and the supersignal enhanced chemiluminescence system (Pierce, Rockford, IL, USA) were used. |
T10936 |
38195-38205 |
NN |
denotes |
Peroxidase |
T10937 |
38206-38213 |
VBN |
denotes |
coupled |
T10938 |
38205-38206 |
HYPH |
denotes |
- |
T10939 |
38224-38234 |
NNS |
denotes |
antibodies |
T10940 |
38214-38223 |
JJ |
denotes |
secondary |
T10941 |
38330-38334 |
VBN |
denotes |
used |
T10942 |
38235-38236 |
-LRB- |
denotes |
( |
T10943 |
38236-38241 |
NNP |
denotes |
Sigma |
T10944 |
38241-38242 |
-RRB- |
denotes |
) |
T10945 |
38243-38246 |
CC |
denotes |
and |
T10946 |
38247-38250 |
DT |
denotes |
the |
T10947 |
38290-38296 |
NN |
denotes |
system |
T10948 |
38251-38262 |
NN |
denotes |
supersignal |
T10949 |
38263-38271 |
VBN |
denotes |
enhanced |
T10950 |
38272-38289 |
NN |
denotes |
chemiluminescence |
T10951 |
38297-38298 |
-LRB- |
denotes |
( |
T10952 |
38298-38304 |
NNP |
denotes |
Pierce |
T10953 |
38304-38306 |
, |
denotes |
, |
T10954 |
38306-38314 |
NNP |
denotes |
Rockford |
T10955 |
38314-38316 |
, |
denotes |
, |
T10956 |
38316-38318 |
NNP |
denotes |
IL |
T10957 |
38318-38320 |
, |
denotes |
, |
T10958 |
38320-38323 |
NNP |
denotes |
USA |
T10959 |
38323-38324 |
-RRB- |
denotes |
) |
T10960 |
38325-38329 |
VBD |
denotes |
were |
T10961 |
38334-38335 |
. |
denotes |
. |
T11182 |
38337-38343 |
NN |
denotes |
Embryo |
T11183 |
38369-38373 |
NN |
denotes |
Blot |
T11184 |
38344-38352 |
JJ |
denotes |
Multiple |
T11185 |
38353-38359 |
NN |
denotes |
Tissue |
T11186 |
38360-38368 |
NNP |
denotes |
Northern |
T11187 |
38373-38496 |
sentence |
denotes |
The presence of Annexin A7 mRNA in mouse ES cells and embryos E7, E11, E15 and E17 was analyzed by northern blot analysis. |
T11188 |
38374-38377 |
DT |
denotes |
The |
T11189 |
38378-38386 |
NN |
denotes |
presence |
T11190 |
38461-38469 |
VBN |
denotes |
analyzed |
T11191 |
38387-38389 |
IN |
denotes |
of |
T11192 |
38390-38397 |
NN |
denotes |
Annexin |
T11193 |
38398-38400 |
NN |
denotes |
A7 |
T11194 |
38401-38405 |
NN |
denotes |
mRNA |
T11195 |
38406-38408 |
IN |
denotes |
in |
T11196 |
38409-38414 |
NN |
denotes |
mouse |
T11197 |
38418-38423 |
NNS |
denotes |
cells |
T11198 |
38415-38417 |
NN |
denotes |
ES |
T11199 |
38424-38427 |
CC |
denotes |
and |
T11200 |
38428-38435 |
NNS |
denotes |
embryos |
T11201 |
38436-38438 |
NN |
denotes |
E7 |
T11202 |
38438-38440 |
, |
denotes |
, |
T11203 |
38440-38443 |
NN |
denotes |
E11 |
T11204 |
38443-38445 |
, |
denotes |
, |
T11205 |
38445-38448 |
NN |
denotes |
E15 |
T11206 |
38449-38452 |
CC |
denotes |
and |
T11207 |
38453-38456 |
NN |
denotes |
E17 |
T11208 |
38457-38460 |
VBD |
denotes |
was |
T11209 |
38470-38472 |
IN |
denotes |
by |
T11210 |
38473-38481 |
NNP |
denotes |
northern |
T11211 |
38482-38486 |
NN |
denotes |
blot |
T11212 |
38487-38495 |
NN |
denotes |
analysis |
T11213 |
38495-38496 |
. |
denotes |
. |
T11214 |
38496-38604 |
sentence |
denotes |
An Embryo Multiple Tissue Northern Blot membrane was obtained from Clontech Laboratories (Heidelberg, FRG). |
T11215 |
38497-38499 |
DT |
denotes |
An |
T11216 |
38537-38545 |
NN |
denotes |
membrane |
T11217 |
38500-38506 |
NN |
denotes |
Embryo |
T11218 |
38507-38515 |
JJ |
denotes |
Multiple |
T11219 |
38516-38522 |
NN |
denotes |
Tissue |
T11220 |
38523-38531 |
NNP |
denotes |
Northern |
T11221 |
38532-38536 |
NN |
denotes |
Blot |
T11222 |
38550-38558 |
VBN |
denotes |
obtained |
T11223 |
38546-38549 |
VBD |
denotes |
was |
T11224 |
38559-38563 |
IN |
denotes |
from |
T11225 |
38564-38572 |
NNP |
denotes |
Clontech |
T11226 |
38573-38585 |
NNP |
denotes |
Laboratories |
T11227 |
38586-38587 |
-LRB- |
denotes |
( |
T11228 |
38587-38597 |
NNP |
denotes |
Heidelberg |
T11229 |
38597-38599 |
, |
denotes |
, |
T11230 |
38599-38602 |
NNP |
denotes |
FRG |
T11231 |
38602-38603 |
-RRB- |
denotes |
) |
T11232 |
38603-38604 |
. |
denotes |
. |
T11233 |
38604-38710 |
sentence |
denotes |
It contained approximately 2 μg of poly(A)+ RNA per lane from four different stages of mouse development. |
T11234 |
38605-38607 |
PRP |
denotes |
It |
T11235 |
38608-38617 |
VBD |
denotes |
contained |
T11236 |
38618-38631 |
RB |
denotes |
approximately |
T11237 |
38632-38633 |
CD |
denotes |
2 |
T11238 |
38634-38636 |
NN |
denotes |
μg |
T11239 |
38637-38639 |
IN |
denotes |
of |
T11240 |
38640-38644 |
NN |
denotes |
poly |
T11241 |
38649-38652 |
NN |
denotes |
RNA |
T11242 |
38644-38645 |
-LRB- |
denotes |
( |
T11243 |
38645-38646 |
NN |
denotes |
A |
T11244 |
38646-38647 |
-RRB- |
denotes |
) |
T11245 |
38647-38648 |
SYM |
denotes |
+ |
T11246 |
38653-38656 |
IN |
denotes |
per |
T11247 |
38657-38661 |
NN |
denotes |
lane |
T11248 |
38662-38666 |
IN |
denotes |
from |
T11249 |
38667-38671 |
CD |
denotes |
four |
T11250 |
38682-38688 |
NNS |
denotes |
stages |
T11251 |
38672-38681 |
JJ |
denotes |
different |
T11252 |
38689-38691 |
IN |
denotes |
of |
T11253 |
38692-38697 |
NN |
denotes |
mouse |
T11254 |
38698-38709 |
NN |
denotes |
development |
T11255 |
38709-38710 |
. |
denotes |
. |
T11256 |
38710-38771 |
sentence |
denotes |
RNA from ES cells was isolated with the RNeasy kit (Qiagen). |
T11257 |
38711-38714 |
NN |
denotes |
RNA |
T11258 |
38733-38741 |
VBN |
denotes |
isolated |
T11259 |
38715-38719 |
IN |
denotes |
from |
T11260 |
38720-38722 |
NN |
denotes |
ES |
T11261 |
38723-38728 |
NNS |
denotes |
cells |
T11262 |
38729-38732 |
VBD |
denotes |
was |
T11263 |
38742-38746 |
IN |
denotes |
with |
T11264 |
38747-38750 |
DT |
denotes |
the |
T11265 |
38758-38761 |
NN |
denotes |
kit |
T11266 |
38751-38757 |
NNP |
denotes |
RNeasy |
T11267 |
38762-38763 |
-LRB- |
denotes |
( |
T11268 |
38763-38769 |
NNP |
denotes |
Qiagen |
T11269 |
38769-38770 |
-RRB- |
denotes |
) |
T11270 |
38770-38771 |
. |
denotes |
. |
T11271 |
38771-38991 |
sentence |
denotes |
The membranes were hybridized with a random-primed [α-32P]-dATP-labelled full length annexin A7 cDNA probe and with a β-actin specific probe for control in ExpressHyb solution according to the manufacturer's directions. |
T11272 |
38772-38775 |
DT |
denotes |
The |
T11273 |
38776-38785 |
NNS |
denotes |
membranes |
T11274 |
38791-38801 |
VBN |
denotes |
hybridized |
T11275 |
38786-38790 |
VBD |
denotes |
were |
T11276 |
38802-38806 |
IN |
denotes |
with |
T11277 |
38807-38808 |
DT |
denotes |
a |
T11278 |
38873-38878 |
NN |
denotes |
probe |
T11279 |
38809-38815 |
JJ |
denotes |
random |
T11280 |
38816-38822 |
VBN |
denotes |
primed |
T11281 |
38815-38816 |
HYPH |
denotes |
- |
T11282 |
38823-38824 |
-LRB- |
denotes |
[ |
T11283 |
38824-38825 |
NN |
denotes |
α |
T11284 |
38825-38826 |
HYPH |
denotes |
- |
T11285 |
38826-38829 |
CD |
denotes |
32P |
T11286 |
38829-38830 |
-RRB- |
denotes |
] |
T11287 |
38830-38831 |
HYPH |
denotes |
- |
T11288 |
38831-38835 |
NN |
denotes |
dATP |
T11289 |
38836-38844 |
VBN |
denotes |
labelled |
T11290 |
38835-38836 |
HYPH |
denotes |
- |
T11291 |
38845-38849 |
JJ |
denotes |
full |
T11292 |
38850-38856 |
NN |
denotes |
length |
T11293 |
38857-38864 |
NN |
denotes |
annexin |
T11294 |
38865-38867 |
NN |
denotes |
A7 |
T11295 |
38868-38872 |
NN |
denotes |
cDNA |
T11296 |
38879-38882 |
CC |
denotes |
and |
T11297 |
38883-38887 |
IN |
denotes |
with |
T11298 |
38888-38889 |
DT |
denotes |
a |
T11299 |
38907-38912 |
NN |
denotes |
probe |
T11300 |
38890-38891 |
NN |
denotes |
β |
T11301 |
38892-38897 |
NN |
denotes |
actin |
T11302 |
38891-38892 |
HYPH |
denotes |
- |
T11303 |
38898-38906 |
JJ |
denotes |
specific |
T11304 |
38913-38916 |
IN |
denotes |
for |
T11305 |
38917-38924 |
NN |
denotes |
control |
T11306 |
38925-38927 |
IN |
denotes |
in |
T11307 |
38928-38938 |
NN |
denotes |
ExpressHyb |
T11308 |
38939-38947 |
NN |
denotes |
solution |
T11309 |
38948-38957 |
VBG |
denotes |
according |
T11310 |
38958-38960 |
IN |
denotes |
to |
T11311 |
38961-38964 |
DT |
denotes |
the |
T11312 |
38965-38977 |
NN |
denotes |
manufacturer |
T11313 |
38980-38990 |
NNS |
denotes |
directions |
T11314 |
38977-38979 |
POS |
denotes |
's |
T11315 |
38990-38991 |
. |
denotes |
. |
T11654 |
5421-5431 |
NN |
denotes |
Expression |
T11655 |
5432-5434 |
IN |
denotes |
of |
T11656 |
5435-5442 |
NN |
denotes |
Annexin |
T11657 |
5443-5445 |
NN |
denotes |
A7 |
T11658 |
5445-5446 |
. |
denotes |
. |
T11659 |
5446-5567 |
sentence |
denotes |
(A) Northern blot analysis of RNA from ES-cells and embryos E7, E11, E15, E17 probed with a full length annexin A7 cDNA. |
T11660 |
5447-5448 |
-LRB- |
denotes |
( |
T11661 |
5448-5449 |
LS |
denotes |
A |
T11662 |
5465-5473 |
NN |
denotes |
analysis |
T11663 |
5449-5450 |
-RRB- |
denotes |
) |
T11664 |
5451-5459 |
NNP |
denotes |
Northern |
T11665 |
5460-5464 |
NN |
denotes |
blot |
T11666 |
5474-5476 |
IN |
denotes |
of |
T11667 |
5477-5480 |
NN |
denotes |
RNA |
T11668 |
5481-5485 |
IN |
denotes |
from |
T11669 |
5486-5488 |
NN |
denotes |
ES |
T11670 |
5489-5494 |
NNS |
denotes |
cells |
T11671 |
5488-5489 |
HYPH |
denotes |
- |
T11672 |
5495-5498 |
CC |
denotes |
and |
T11673 |
5499-5506 |
NNS |
denotes |
embryos |
T11674 |
5521-5524 |
NN |
denotes |
E17 |
T11675 |
5507-5509 |
NN |
denotes |
E7 |
T11676 |
5509-5511 |
, |
denotes |
, |
T11677 |
5511-5514 |
NN |
denotes |
E11 |
T11678 |
5514-5516 |
, |
denotes |
, |
T11679 |
5516-5519 |
NN |
denotes |
E15 |
T11680 |
5519-5521 |
, |
denotes |
, |
T11681 |
5525-5531 |
VBD |
denotes |
probed |
T11682 |
5532-5536 |
IN |
denotes |
with |
T11683 |
5537-5538 |
DT |
denotes |
a |
T11684 |
5562-5566 |
NN |
denotes |
cDNA |
T11685 |
5539-5543 |
JJ |
denotes |
full |
T11686 |
5544-5550 |
NN |
denotes |
length |
T11687 |
5551-5558 |
NN |
denotes |
annexin |
T11688 |
5559-5561 |
NN |
denotes |
A7 |
T11689 |
5566-5567 |
. |
denotes |
. |
T11690 |
5567-5648 |
sentence |
denotes |
Transcripts of 1.8 and 2.4 kb are detected in all stages examined (upper panel). |
T11691 |
5568-5579 |
NNS |
denotes |
Transcripts |
T11692 |
5602-5610 |
VBN |
denotes |
detected |
T11693 |
5580-5582 |
IN |
denotes |
of |
T11694 |
5583-5586 |
CD |
denotes |
1.8 |
T11695 |
5595-5597 |
NN |
denotes |
kb |
T11696 |
5587-5590 |
CC |
denotes |
and |
T11697 |
5591-5594 |
CD |
denotes |
2.4 |
T11698 |
5598-5601 |
VBP |
denotes |
are |
T11699 |
5611-5613 |
IN |
denotes |
in |
T11700 |
5614-5617 |
DT |
denotes |
all |
T11701 |
5618-5624 |
NNS |
denotes |
stages |
T11702 |
5625-5633 |
VBN |
denotes |
examined |
T11703 |
5634-5635 |
-LRB- |
denotes |
( |
T11704 |
5641-5646 |
NN |
denotes |
panel |
T11705 |
5635-5640 |
JJ |
denotes |
upper |
T11706 |
5646-5647 |
-RRB- |
denotes |
) |
T11707 |
5647-5648 |
. |
denotes |
. |
T11708 |
5648-5730 |
sentence |
denotes |
Reprobing with a β-actin probe verified equal loading (lower panel, 2.0 kb band). |
T11709 |
5649-5658 |
VBG |
denotes |
Reprobing |
T11710 |
5680-5688 |
VBD |
denotes |
verified |
T11711 |
5659-5663 |
IN |
denotes |
with |
T11712 |
5664-5665 |
DT |
denotes |
a |
T11713 |
5674-5679 |
NN |
denotes |
probe |
T11714 |
5666-5667 |
NN |
denotes |
β |
T11715 |
5668-5673 |
NN |
denotes |
actin |
T11716 |
5667-5668 |
HYPH |
denotes |
- |
T11717 |
5689-5694 |
JJ |
denotes |
equal |
T11718 |
5695-5702 |
NN |
denotes |
loading |
T11719 |
5703-5704 |
-LRB- |
denotes |
( |
T11720 |
5724-5728 |
NN |
denotes |
band |
T11721 |
5704-5709 |
JJ |
denotes |
lower |
T11722 |
5710-5715 |
NN |
denotes |
panel |
T11723 |
5715-5717 |
, |
denotes |
, |
T11724 |
5717-5720 |
CD |
denotes |
2.0 |
T11725 |
5721-5723 |
NN |
denotes |
kb |
T11726 |
5728-5729 |
-RRB- |
denotes |
) |
T11727 |
5729-5730 |
. |
denotes |
. |
T11728 |
5730-5798 |
sentence |
denotes |
(B) Murine ES cells (ES) express only the small Annexin A7 isoform. |
T11729 |
5731-5732 |
-LRB- |
denotes |
( |
T11730 |
5732-5733 |
LS |
denotes |
B |
T11731 |
5756-5763 |
VBP |
denotes |
express |
T11732 |
5733-5734 |
-RRB- |
denotes |
) |
T11733 |
5735-5741 |
JJ |
denotes |
Murine |
T11734 |
5745-5750 |
NNS |
denotes |
cells |
T11735 |
5742-5744 |
NN |
denotes |
ES |
T11736 |
5751-5752 |
-LRB- |
denotes |
( |
T11737 |
5752-5754 |
NN |
denotes |
ES |
T11738 |
5754-5755 |
-RRB- |
denotes |
) |
T11739 |
5764-5768 |
RB |
denotes |
only |
T11740 |
5790-5797 |
NN |
denotes |
isoform |
T11741 |
5769-5772 |
DT |
denotes |
the |
T11742 |
5773-5778 |
JJ |
denotes |
small |
T11743 |
5779-5786 |
NN |
denotes |
Annexin |
T11744 |
5787-5789 |
NN |
denotes |
A7 |
T11745 |
5797-5798 |
. |
denotes |
. |
T11746 |
5798-5917 |
sentence |
denotes |
Both isoforms are expressed in neuroblastoma neuro-2a cells (N2A), heart (HRT) and brain tissue (CNS) from adult mice. |
T11747 |
5799-5803 |
DT |
denotes |
Both |
T11748 |
5804-5812 |
NNS |
denotes |
isoforms |
T11749 |
5817-5826 |
VBN |
denotes |
expressed |
T11750 |
5813-5816 |
VBP |
denotes |
are |
T11751 |
5827-5829 |
IN |
denotes |
in |
T11752 |
5830-5843 |
NN |
denotes |
neuroblastoma |
T11753 |
5853-5858 |
NNS |
denotes |
cells |
T11754 |
5844-5849 |
NN |
denotes |
neuro |
T11755 |
5850-5852 |
NN |
denotes |
2a |
T11756 |
5849-5850 |
HYPH |
denotes |
- |
T11757 |
5859-5860 |
-LRB- |
denotes |
( |
T11758 |
5860-5863 |
NN |
denotes |
N2A |
T11759 |
5863-5864 |
-RRB- |
denotes |
) |
T11760 |
5864-5866 |
, |
denotes |
, |
T11761 |
5866-5871 |
NN |
denotes |
heart |
T11762 |
5872-5873 |
-LRB- |
denotes |
( |
T11763 |
5873-5876 |
NN |
denotes |
HRT |
T11764 |
5876-5877 |
-RRB- |
denotes |
) |
T11765 |
5878-5881 |
CC |
denotes |
and |
T11766 |
5882-5887 |
NN |
denotes |
brain |
T11767 |
5888-5894 |
NN |
denotes |
tissue |
T11768 |
5895-5896 |
-LRB- |
denotes |
( |
T11769 |
5896-5899 |
NN |
denotes |
CNS |
T11770 |
5899-5900 |
-RRB- |
denotes |
) |
T11771 |
5901-5905 |
IN |
denotes |
from |
T11772 |
5906-5911 |
JJ |
denotes |
adult |
T11773 |
5912-5916 |
NNS |
denotes |
mice |
T11774 |
5916-5917 |
. |
denotes |
. |
T11775 |
5917-6088 |
sentence |
denotes |
Proteins from neuro-2a and ES cells, heart and brain were resolved by SDS polyacrylamide gel electrophoresis (12% acrylamide) and transferred to nitrocellulose membranes. |
T11776 |
5918-5926 |
NNS |
denotes |
Proteins |
T11777 |
5976-5984 |
VBN |
denotes |
resolved |
T11778 |
5927-5931 |
IN |
denotes |
from |
T11779 |
5932-5937 |
NN |
denotes |
neuro |
T11780 |
5938-5940 |
NN |
denotes |
2a |
T11781 |
5937-5938 |
HYPH |
denotes |
- |
T11782 |
5941-5944 |
CC |
denotes |
and |
T11783 |
5945-5947 |
NN |
denotes |
ES |
T11784 |
5948-5953 |
NNS |
denotes |
cells |
T11785 |
5953-5955 |
, |
denotes |
, |
T11786 |
5955-5960 |
NN |
denotes |
heart |
T11787 |
5961-5964 |
CC |
denotes |
and |
T11788 |
5965-5970 |
NN |
denotes |
brain |
T11789 |
5971-5975 |
VBD |
denotes |
were |
T11790 |
5985-5987 |
IN |
denotes |
by |
T11791 |
5988-5991 |
NN |
denotes |
SDS |
T11792 |
6011-6026 |
NN |
denotes |
electrophoresis |
T11793 |
5992-6006 |
NN |
denotes |
polyacrylamide |
T11794 |
6007-6010 |
NN |
denotes |
gel |
T11795 |
6027-6028 |
-LRB- |
denotes |
( |
T11796 |
6032-6042 |
NN |
denotes |
acrylamide |
T11797 |
6028-6030 |
CD |
denotes |
12 |
T11798 |
6030-6031 |
NN |
denotes |
% |
T11799 |
6042-6043 |
-RRB- |
denotes |
) |
T11800 |
6044-6047 |
CC |
denotes |
and |
T11801 |
6048-6059 |
VBN |
denotes |
transferred |
T11802 |
6060-6062 |
IN |
denotes |
to |
T11803 |
6063-6077 |
NN |
denotes |
nitrocellulose |
T11804 |
6078-6087 |
NNS |
denotes |
membranes |
T11805 |
6087-6088 |
. |
denotes |
. |
T11806 |
6088-6202 |
sentence |
denotes |
The Western blot was probed with mAb 203–217 followed by incubation with a peroxidase coupled secondary antibody. |
T11807 |
6089-6092 |
DT |
denotes |
The |
T11808 |
6101-6105 |
NN |
denotes |
blot |
T11809 |
6093-6100 |
NNP |
denotes |
Western |
T11810 |
6110-6116 |
VBN |
denotes |
probed |
T11811 |
6106-6109 |
VBD |
denotes |
was |
T11812 |
6117-6121 |
IN |
denotes |
with |
T11813 |
6122-6125 |
NN |
denotes |
mAb |
T11814 |
6126-6129 |
CD |
denotes |
203 |
T11815 |
6129-6130 |
SYM |
denotes |
– |
T11816 |
6130-6133 |
CD |
denotes |
217 |
T11817 |
6134-6142 |
VBN |
denotes |
followed |
T11818 |
6143-6145 |
IN |
denotes |
by |
T11819 |
6146-6156 |
NN |
denotes |
incubation |
T11820 |
6157-6161 |
IN |
denotes |
with |
T11821 |
6162-6163 |
DT |
denotes |
a |
T11822 |
6193-6201 |
NN |
denotes |
antibody |
T11823 |
6164-6174 |
NN |
denotes |
peroxidase |
T11824 |
6175-6182 |
VBN |
denotes |
coupled |
T11825 |
6183-6192 |
JJ |
denotes |
secondary |
T11826 |
6201-6202 |
. |
denotes |
. |
T11827 |
6202-6249 |
sentence |
denotes |
Detection was with enhanced chemiluminescence. |
T11828 |
6203-6212 |
NN |
denotes |
Detection |
T11829 |
6213-6216 |
VBD |
denotes |
was |
T11830 |
6217-6221 |
IN |
denotes |
with |
T11831 |
6222-6230 |
VBN |
denotes |
enhanced |
T11832 |
6231-6248 |
NN |
denotes |
chemiluminescence |
T11833 |
6248-6249 |
. |
denotes |
. |
T12193 |
7151-7158 |
NN |
denotes |
Annexin |
T12194 |
7159-7161 |
NN |
denotes |
A7 |
T12195 |
7162-7178 |
NN |
denotes |
immunoreactivity |
T12196 |
7179-7181 |
IN |
denotes |
in |
T12197 |
7182-7187 |
JJ |
denotes |
early |
T12198 |
7194-7201 |
NNS |
denotes |
embryos |
T12199 |
7188-7193 |
NN |
denotes |
mouse |
T12200 |
7201-7202 |
. |
denotes |
. |
T12201 |
7202-7353 |
sentence |
denotes |
(A) Phase contrast, embryo E5: The egg cylinder consists of an inner cell mass (a) representing the ectoderm and an outer layer of endoderm cells (b). |
T12202 |
7203-7204 |
-LRB- |
denotes |
( |
T12203 |
7204-7205 |
LS |
denotes |
A |
T12204 |
7251-7259 |
VBZ |
denotes |
consists |
T12205 |
7205-7206 |
-RRB- |
denotes |
) |
T12206 |
7207-7212 |
NN |
denotes |
Phase |
T12207 |
7213-7221 |
NN |
denotes |
contrast |
T12208 |
7221-7223 |
, |
denotes |
, |
T12209 |
7223-7229 |
NN |
denotes |
embryo |
T12210 |
7230-7232 |
NN |
denotes |
E5 |
T12211 |
7232-7234 |
: |
denotes |
: |
T12212 |
7234-7237 |
DT |
denotes |
The |
T12213 |
7242-7250 |
NN |
denotes |
cylinder |
T12214 |
7238-7241 |
NN |
denotes |
egg |
T12215 |
7260-7262 |
IN |
denotes |
of |
T12216 |
7263-7265 |
DT |
denotes |
an |
T12217 |
7277-7281 |
NN |
denotes |
mass |
T12218 |
7266-7271 |
JJ |
denotes |
inner |
T12219 |
7272-7276 |
NN |
denotes |
cell |
T12220 |
7282-7283 |
-LRB- |
denotes |
( |
T12221 |
7283-7284 |
NN |
denotes |
a |
T12222 |
7284-7285 |
-RRB- |
denotes |
) |
T12223 |
7286-7298 |
VBG |
denotes |
representing |
T12224 |
7299-7302 |
DT |
denotes |
the |
T12225 |
7303-7311 |
NN |
denotes |
ectoderm |
T12226 |
7312-7315 |
CC |
denotes |
and |
T12227 |
7316-7318 |
DT |
denotes |
an |
T12228 |
7325-7330 |
NN |
denotes |
layer |
T12229 |
7319-7324 |
JJ |
denotes |
outer |
T12230 |
7331-7333 |
IN |
denotes |
of |
T12231 |
7334-7342 |
NN |
denotes |
endoderm |
T12232 |
7343-7348 |
NNS |
denotes |
cells |
T12233 |
7349-7350 |
-LRB- |
denotes |
( |
T12234 |
7350-7351 |
NN |
denotes |
b |
T12235 |
7351-7352 |
-RRB- |
denotes |
) |
T12236 |
7352-7353 |
. |
denotes |
. |
T12237 |
7353-7472 |
sentence |
denotes |
(B) Immunostaining of the paraffin section was performed using purified mAb 203–217 and Cy3-conjugated anti-mouse IgG. |
T12238 |
7354-7355 |
-LRB- |
denotes |
( |
T12239 |
7355-7356 |
LS |
denotes |
B |
T12240 |
7401-7410 |
VBN |
denotes |
performed |
T12241 |
7356-7357 |
-RRB- |
denotes |
) |
T12242 |
7358-7372 |
NN |
denotes |
Immunostaining |
T12243 |
7373-7375 |
IN |
denotes |
of |
T12244 |
7376-7379 |
DT |
denotes |
the |
T12245 |
7389-7396 |
NN |
denotes |
section |
T12246 |
7380-7388 |
NN |
denotes |
paraffin |
T12247 |
7397-7400 |
VBD |
denotes |
was |
T12248 |
7411-7416 |
VBG |
denotes |
using |
T12249 |
7417-7425 |
VBN |
denotes |
purified |
T12250 |
7426-7429 |
NN |
denotes |
mAb |
T12251 |
7430-7433 |
CD |
denotes |
203 |
T12252 |
7433-7434 |
SYM |
denotes |
– |
T12253 |
7434-7437 |
CD |
denotes |
217 |
T12254 |
7438-7441 |
CC |
denotes |
and |
T12255 |
7442-7445 |
NN |
denotes |
Cy3 |
T12256 |
7446-7456 |
VBN |
denotes |
conjugated |
T12257 |
7445-7446 |
HYPH |
denotes |
- |
T12258 |
7468-7471 |
NN |
denotes |
IgG |
T12259 |
7457-7467 |
JJ |
denotes |
anti-mouse |
T12260 |
7471-7472 |
. |
denotes |
. |
T12261 |
7472-7613 |
sentence |
denotes |
Annexin A7 is expressed in both cell types of the egg cylinder with a strong staining of the endoderm and a weaker staining of the ectoderm. |
T12262 |
7473-7480 |
NN |
denotes |
Annexin |
T12263 |
7481-7483 |
NN |
denotes |
A7 |
T12264 |
7487-7496 |
VBN |
denotes |
expressed |
T12265 |
7484-7486 |
VBZ |
denotes |
is |
T12266 |
7497-7499 |
IN |
denotes |
in |
T12267 |
7500-7504 |
DT |
denotes |
both |
T12268 |
7510-7515 |
NNS |
denotes |
types |
T12269 |
7505-7509 |
NN |
denotes |
cell |
T12270 |
7516-7518 |
IN |
denotes |
of |
T12271 |
7519-7522 |
DT |
denotes |
the |
T12272 |
7527-7535 |
NN |
denotes |
cylinder |
T12273 |
7523-7526 |
NN |
denotes |
egg |
T12274 |
7536-7540 |
IN |
denotes |
with |
T12275 |
7541-7542 |
DT |
denotes |
a |
T12276 |
7550-7558 |
NN |
denotes |
staining |
T12277 |
7543-7549 |
JJ |
denotes |
strong |
T12278 |
7559-7561 |
IN |
denotes |
of |
T12279 |
7562-7565 |
DT |
denotes |
the |
T12280 |
7566-7574 |
NN |
denotes |
endoderm |
T12281 |
7575-7578 |
CC |
denotes |
and |
T12282 |
7579-7580 |
DT |
denotes |
a |
T12283 |
7588-7596 |
NN |
denotes |
staining |
T12284 |
7581-7587 |
JJR |
denotes |
weaker |
T12285 |
7597-7599 |
IN |
denotes |
of |
T12286 |
7600-7603 |
DT |
denotes |
the |
T12287 |
7604-7612 |
NN |
denotes |
ectoderm |
T12288 |
7612-7613 |
. |
denotes |
. |
T12289 |
7613-7656 |
sentence |
denotes |
The nuclei are devoid of immune reactions. |
T12290 |
7614-7617 |
DT |
denotes |
The |
T12291 |
7618-7624 |
NNS |
denotes |
nuclei |
T12292 |
7625-7628 |
VBP |
denotes |
are |
T12293 |
7629-7635 |
JJ |
denotes |
devoid |
T12294 |
7636-7638 |
IN |
denotes |
of |
T12295 |
7639-7645 |
JJ |
denotes |
immune |
T12296 |
7646-7655 |
NNS |
denotes |
reactions |
T12297 |
7655-7656 |
. |
denotes |
. |
T12298 |
7656-7716 |
sentence |
denotes |
(C) Negative control using the secondary Cy3-antibody only. |
T12299 |
7657-7658 |
-LRB- |
denotes |
( |
T12300 |
7670-7677 |
NN |
denotes |
control |
T12301 |
7658-7659 |
LS |
denotes |
C |
T12302 |
7659-7660 |
-RRB- |
denotes |
) |
T12303 |
7661-7669 |
JJ |
denotes |
Negative |
T12304 |
7678-7683 |
VBG |
denotes |
using |
T12305 |
7684-7687 |
DT |
denotes |
the |
T12306 |
7702-7710 |
NN |
denotes |
antibody |
T12307 |
7688-7697 |
JJ |
denotes |
secondary |
T12308 |
7698-7701 |
NN |
denotes |
Cy3 |
T12309 |
7701-7702 |
HYPH |
denotes |
- |
T12310 |
7711-7715 |
RB |
denotes |
only |
T12311 |
7715-7716 |
. |
denotes |
. |
T12312 |
7716-7837 |
sentence |
denotes |
(D-F) Annexin A7 expression in the proximal neural tube (D) and nearby neural fold (E,F), embryo E8, transverse section. |
T12313 |
7717-7718 |
-LRB- |
denotes |
( |
T12314 |
7718-7719 |
LS |
denotes |
D |
T12315 |
7829-7836 |
NN |
denotes |
section |
T12316 |
7719-7720 |
SYM |
denotes |
- |
T12317 |
7720-7721 |
LS |
denotes |
F |
T12318 |
7721-7722 |
-RRB- |
denotes |
) |
T12319 |
7723-7730 |
NN |
denotes |
Annexin |
T12320 |
7731-7733 |
NN |
denotes |
A7 |
T12321 |
7734-7744 |
NN |
denotes |
expression |
T12322 |
7745-7747 |
IN |
denotes |
in |
T12323 |
7748-7751 |
DT |
denotes |
the |
T12324 |
7768-7772 |
NN |
denotes |
tube |
T12325 |
7752-7760 |
JJ |
denotes |
proximal |
T12326 |
7761-7767 |
JJ |
denotes |
neural |
T12327 |
7773-7774 |
-LRB- |
denotes |
( |
T12328 |
7774-7775 |
NN |
denotes |
D |
T12329 |
7775-7776 |
-RRB- |
denotes |
) |
T12330 |
7777-7780 |
CC |
denotes |
and |
T12331 |
7781-7787 |
JJ |
denotes |
nearby |
T12332 |
7795-7799 |
NN |
denotes |
fold |
T12333 |
7788-7794 |
JJ |
denotes |
neural |
T12334 |
7800-7801 |
-LRB- |
denotes |
( |
T12335 |
7803-7804 |
NN |
denotes |
F |
T12336 |
7801-7802 |
NN |
denotes |
E |
T12337 |
7802-7803 |
, |
denotes |
, |
T12338 |
7804-7805 |
-RRB- |
denotes |
) |
T12339 |
7805-7807 |
, |
denotes |
, |
T12340 |
7807-7813 |
NN |
denotes |
embryo |
T12341 |
7814-7816 |
NN |
denotes |
E8 |
T12342 |
7816-7818 |
, |
denotes |
, |
T12343 |
7818-7828 |
JJ |
denotes |
transverse |
T12344 |
7836-7837 |
. |
denotes |
. |
T12345 |
7837-7979 |
sentence |
denotes |
Immunolabeling of Annexin A7 was performed with purified mAb 203–217 and visualization was with an Alexa Fluor 488-conjugated anti-mouse IgG. |
T12346 |
7838-7852 |
NN |
denotes |
Immunolabeling |
T12347 |
7871-7880 |
VBN |
denotes |
performed |
T12348 |
7853-7855 |
IN |
denotes |
of |
T12349 |
7856-7863 |
NN |
denotes |
Annexin |
T12350 |
7864-7866 |
NN |
denotes |
A7 |
T12351 |
7867-7870 |
VBD |
denotes |
was |
T12352 |
7881-7885 |
IN |
denotes |
with |
T12353 |
7886-7894 |
VBN |
denotes |
purified |
T12354 |
7899-7902 |
CD |
denotes |
203 |
T12355 |
7895-7898 |
NN |
denotes |
mAb |
T12356 |
7902-7903 |
SYM |
denotes |
– |
T12357 |
7903-7906 |
CD |
denotes |
217 |
T12358 |
7907-7910 |
CC |
denotes |
and |
T12359 |
7911-7924 |
NN |
denotes |
visualization |
T12360 |
7925-7928 |
VBD |
denotes |
was |
T12361 |
7929-7933 |
IN |
denotes |
with |
T12362 |
7934-7936 |
DT |
denotes |
an |
T12363 |
7975-7978 |
NN |
denotes |
IgG |
T12364 |
7937-7942 |
NNP |
denotes |
Alexa |
T12365 |
7943-7948 |
NNP |
denotes |
Fluor |
T12366 |
7953-7963 |
VBN |
denotes |
conjugated |
T12367 |
7949-7952 |
CD |
denotes |
488 |
T12368 |
7952-7953 |
HYPH |
denotes |
- |
T12369 |
7964-7974 |
JJ |
denotes |
anti-mouse |
T12370 |
7978-7979 |
. |
denotes |
. |
T12371 |
7979-8103 |
sentence |
denotes |
(D) An intense Annexin A7 immunostaining is detectable in the neuroepithelium of the neural tube (a, lumen of neural tube). |
T12372 |
7980-7981 |
-LRB- |
denotes |
( |
T12373 |
7981-7982 |
LS |
denotes |
D |
T12374 |
8021-8023 |
VBZ |
denotes |
is |
T12375 |
7982-7983 |
-RRB- |
denotes |
) |
T12376 |
7984-7986 |
DT |
denotes |
An |
T12377 |
8006-8020 |
NN |
denotes |
immunostaining |
T12378 |
7987-7994 |
JJ |
denotes |
intense |
T12379 |
7995-8002 |
NN |
denotes |
Annexin |
T12380 |
8003-8005 |
NN |
denotes |
A7 |
T12381 |
8024-8034 |
JJ |
denotes |
detectable |
T12382 |
8035-8037 |
IN |
denotes |
in |
T12383 |
8038-8041 |
DT |
denotes |
the |
T12384 |
8042-8057 |
NN |
denotes |
neuroepithelium |
T12385 |
8058-8060 |
IN |
denotes |
of |
T12386 |
8061-8064 |
DT |
denotes |
the |
T12387 |
8072-8076 |
NN |
denotes |
tube |
T12388 |
8065-8071 |
JJ |
denotes |
neural |
T12389 |
8077-8078 |
-LRB- |
denotes |
( |
T12390 |
8081-8086 |
NN |
denotes |
lumen |
T12391 |
8078-8079 |
NN |
denotes |
a |
T12392 |
8079-8081 |
, |
denotes |
, |
T12393 |
8087-8089 |
IN |
denotes |
of |
T12394 |
8090-8096 |
JJ |
denotes |
neural |
T12395 |
8097-8101 |
NN |
denotes |
tube |
T12396 |
8101-8102 |
-RRB- |
denotes |
) |
T12397 |
8102-8103 |
. |
denotes |
. |
T12398 |
8103-8204 |
sentence |
denotes |
(E,F) Higher magnifications of the neuroepithelium show that Annexin A7 is expressed in the cytosol. |
T12399 |
8104-8105 |
-LRB- |
denotes |
( |
T12400 |
8105-8106 |
LS |
denotes |
E |
T12401 |
8155-8159 |
VBP |
denotes |
show |
T12402 |
8106-8107 |
, |
denotes |
, |
T12403 |
8107-8108 |
LS |
denotes |
F |
T12404 |
8108-8109 |
-RRB- |
denotes |
) |
T12405 |
8110-8116 |
JJR |
denotes |
Higher |
T12406 |
8117-8131 |
NNS |
denotes |
magnifications |
T12407 |
8132-8134 |
IN |
denotes |
of |
T12408 |
8135-8138 |
DT |
denotes |
the |
T12409 |
8139-8154 |
NN |
denotes |
neuroepithelium |
T12410 |
8160-8164 |
IN |
denotes |
that |
T12411 |
8179-8188 |
VBN |
denotes |
expressed |
T12412 |
8165-8172 |
NN |
denotes |
Annexin |
T12413 |
8173-8175 |
NN |
denotes |
A7 |
T12414 |
8176-8178 |
VBZ |
denotes |
is |
T12415 |
8189-8191 |
IN |
denotes |
in |
T12416 |
8192-8195 |
DT |
denotes |
the |
T12417 |
8196-8203 |
NN |
denotes |
cytosol |
T12418 |
8203-8204 |
. |
denotes |
. |
T12419 |
8204-8268 |
sentence |
denotes |
Arrowheads point to Annexin A7 immunoreactivity in the cytosol. |
T12420 |
8205-8215 |
NNS |
denotes |
Arrowheads |
T12421 |
8216-8221 |
VBP |
denotes |
point |
T12422 |
8222-8224 |
IN |
denotes |
to |
T12423 |
8225-8232 |
NN |
denotes |
Annexin |
T12424 |
8233-8235 |
NN |
denotes |
A7 |
T12425 |
8236-8252 |
NN |
denotes |
immunoreactivity |
T12426 |
8253-8255 |
IN |
denotes |
in |
T12427 |
8256-8259 |
DT |
denotes |
the |
T12428 |
8260-8267 |
NN |
denotes |
cytosol |
T12429 |
8267-8268 |
. |
denotes |
. |
T12430 |
8268-8320 |
sentence |
denotes |
(G) Phase contrast, embryo E13, caudal neural tube. |
T12431 |
8269-8270 |
-LRB- |
denotes |
( |
T12432 |
8315-8319 |
NN |
denotes |
tube |
T12433 |
8270-8271 |
LS |
denotes |
G |
T12434 |
8271-8272 |
-RRB- |
denotes |
) |
T12435 |
8273-8278 |
NN |
denotes |
Phase |
T12436 |
8279-8287 |
NN |
denotes |
contrast |
T12437 |
8287-8289 |
, |
denotes |
, |
T12438 |
8289-8295 |
NN |
denotes |
embryo |
T12439 |
8296-8299 |
NN |
denotes |
E13 |
T12440 |
8299-8301 |
, |
denotes |
, |
T12441 |
8301-8307 |
JJ |
denotes |
caudal |
T12442 |
8308-8314 |
JJ |
denotes |
neural |
T12443 |
8319-8320 |
. |
denotes |
. |
T12444 |
8320-8451 |
sentence |
denotes |
(H) Immunostaining of the paraffin section was performed using purified mAb 203–217 and Alexa Fluor 488-conjugated anti-mouse IgG. |
T12445 |
8321-8322 |
-LRB- |
denotes |
( |
T12446 |
8322-8323 |
LS |
denotes |
H |
T12447 |
8368-8377 |
VBN |
denotes |
performed |
T12448 |
8323-8324 |
-RRB- |
denotes |
) |
T12449 |
8325-8339 |
NN |
denotes |
Immunostaining |
T12450 |
8340-8342 |
IN |
denotes |
of |
T12451 |
8343-8346 |
DT |
denotes |
the |
T12452 |
8356-8363 |
NN |
denotes |
section |
T12453 |
8347-8355 |
NN |
denotes |
paraffin |
T12454 |
8364-8367 |
VBD |
denotes |
was |
T12455 |
8378-8383 |
VBG |
denotes |
using |
T12456 |
8384-8392 |
VBN |
denotes |
purified |
T12457 |
8397-8400 |
CD |
denotes |
203 |
T12458 |
8393-8396 |
NN |
denotes |
mAb |
T12459 |
8400-8401 |
SYM |
denotes |
– |
T12460 |
8401-8404 |
CD |
denotes |
217 |
T12461 |
8405-8408 |
CC |
denotes |
and |
T12462 |
8409-8414 |
NNP |
denotes |
Alexa |
T12463 |
8415-8420 |
NNP |
denotes |
Fluor |
T12464 |
8425-8435 |
VBN |
denotes |
conjugated |
T12465 |
8421-8424 |
CD |
denotes |
488 |
T12466 |
8424-8425 |
HYPH |
denotes |
- |
T12467 |
8447-8450 |
NN |
denotes |
IgG |
T12468 |
8436-8446 |
JJ |
denotes |
anti-mouse |
T12469 |
8450-8451 |
. |
denotes |
. |
T12470 |
8451-8523 |
sentence |
denotes |
(I) Negative control using the secondary Alexa Fluor 488-antibody only. |
T12471 |
8452-8453 |
-LRB- |
denotes |
( |
T12472 |
8453-8454 |
LS |
denotes |
I |
T12473 |
8465-8472 |
NN |
denotes |
control |
T12474 |
8454-8455 |
-RRB- |
denotes |
) |
T12475 |
8456-8464 |
JJ |
denotes |
Negative |
T12476 |
8473-8478 |
VBG |
denotes |
using |
T12477 |
8479-8482 |
DT |
denotes |
the |
T12478 |
8509-8517 |
NN |
denotes |
antibody |
T12479 |
8483-8492 |
JJ |
denotes |
secondary |
T12480 |
8493-8498 |
NNP |
denotes |
Alexa |
T12481 |
8499-8504 |
NNP |
denotes |
Fluor |
T12482 |
8505-8508 |
CD |
denotes |
488 |
T12483 |
8508-8509 |
HYPH |
denotes |
- |
T12484 |
8518-8522 |
RB |
denotes |
only |
T12485 |
8522-8523 |
. |
denotes |
. |
T12486 |
8523-8535 |
sentence |
denotes |
Bar, 20 μm. |
T12487 |
8524-8527 |
NN |
denotes |
Bar |
T12488 |
8527-8529 |
, |
denotes |
, |
T12489 |
8529-8531 |
CD |
denotes |
20 |
T12490 |
8532-8534 |
NN |
denotes |
μm |
T12491 |
8534-8535 |
. |
denotes |
. |
T12790 |
9700-9711 |
JJ |
denotes |
Subcellular |
T12791 |
9712-9724 |
NN |
denotes |
localization |
T12792 |
9725-9727 |
IN |
denotes |
of |
T12793 |
9728-9735 |
NN |
denotes |
Annexin |
T12794 |
9736-9738 |
NN |
denotes |
A7 |
T12795 |
9739-9741 |
IN |
denotes |
in |
T12796 |
9742-9749 |
NNS |
denotes |
embryos |
T12797 |
9750-9753 |
NN |
denotes |
E13 |
T12798 |
9753-9755 |
, |
denotes |
, |
T12799 |
9755-9758 |
NN |
denotes |
E15 |
T12800 |
9759-9762 |
CC |
denotes |
and |
T12801 |
9763-9766 |
NN |
denotes |
E16 |
T12802 |
9766-9767 |
. |
denotes |
. |
T12803 |
9767-9844 |
sentence |
denotes |
Paraffin sections of embryonic brain were stained with purified mAb 203–217. |
T12804 |
9768-9776 |
NN |
denotes |
Paraffin |
T12805 |
9777-9785 |
NNS |
denotes |
sections |
T12806 |
9810-9817 |
VBN |
denotes |
stained |
T12807 |
9786-9788 |
IN |
denotes |
of |
T12808 |
9789-9798 |
JJ |
denotes |
embryonic |
T12809 |
9799-9804 |
NN |
denotes |
brain |
T12810 |
9805-9809 |
VBD |
denotes |
were |
T12811 |
9818-9822 |
IN |
denotes |
with |
T12812 |
9823-9831 |
VBN |
denotes |
purified |
T12813 |
9836-9839 |
CD |
denotes |
203 |
T12814 |
9832-9835 |
NN |
denotes |
mAb |
T12815 |
9839-9840 |
SYM |
denotes |
– |
T12816 |
9840-9843 |
CD |
denotes |
217 |
T12817 |
9843-9844 |
. |
denotes |
. |
T12818 |
9844-9922 |
sentence |
denotes |
Annexin A7 was visualized with Alexa Fluor 488-conjugated secondary antibody. |
T12819 |
9845-9852 |
NN |
denotes |
Annexin |
T12820 |
9853-9855 |
NN |
denotes |
A7 |
T12821 |
9860-9870 |
VBN |
denotes |
visualized |
T12822 |
9856-9859 |
VBD |
denotes |
was |
T12823 |
9871-9875 |
IN |
denotes |
with |
T12824 |
9876-9881 |
NNP |
denotes |
Alexa |
T12825 |
9882-9887 |
NNP |
denotes |
Fluor |
T12826 |
9892-9902 |
VBN |
denotes |
conjugated |
T12827 |
9888-9891 |
CD |
denotes |
488 |
T12828 |
9891-9892 |
HYPH |
denotes |
- |
T12829 |
9913-9921 |
NN |
denotes |
antibody |
T12830 |
9903-9912 |
JJ |
denotes |
secondary |
T12831 |
9921-9922 |
. |
denotes |
. |
T12832 |
9922-10135 |
sentence |
denotes |
(A) Overview, at E16 the immature GFAP-negative cells of the forming cerebral neocortex (b) surrounding the lateral ventricle (a) are strongly stained; square, a higher magnification of this area is given in (I). |
T12833 |
9923-9924 |
-LRB- |
denotes |
( |
T12834 |
9924-9925 |
LS |
denotes |
A |
T12835 |
10122-10127 |
VBN |
denotes |
given |
T12836 |
9925-9926 |
-RRB- |
denotes |
) |
T12837 |
9927-9935 |
NN |
denotes |
Overview |
T12838 |
10066-10073 |
VBN |
denotes |
stained |
T12839 |
9935-9937 |
, |
denotes |
, |
T12840 |
9937-9939 |
IN |
denotes |
at |
T12841 |
9940-9943 |
NN |
denotes |
E16 |
T12842 |
9944-9947 |
DT |
denotes |
the |
T12843 |
9971-9976 |
NNS |
denotes |
cells |
T12844 |
9948-9956 |
JJ |
denotes |
immature |
T12845 |
9957-9961 |
NN |
denotes |
GFAP |
T12846 |
9962-9970 |
JJ |
denotes |
negative |
T12847 |
9961-9962 |
HYPH |
denotes |
- |
T12848 |
9977-9979 |
IN |
denotes |
of |
T12849 |
9980-9983 |
DT |
denotes |
the |
T12850 |
10001-10010 |
NN |
denotes |
neocortex |
T12851 |
9984-9991 |
VBG |
denotes |
forming |
T12852 |
9992-10000 |
JJ |
denotes |
cerebral |
T12853 |
10011-10012 |
-LRB- |
denotes |
( |
T12854 |
10012-10013 |
NN |
denotes |
b |
T12855 |
10013-10014 |
-RRB- |
denotes |
) |
T12856 |
10015-10026 |
VBG |
denotes |
surrounding |
T12857 |
10027-10030 |
DT |
denotes |
the |
T12858 |
10039-10048 |
NN |
denotes |
ventricle |
T12859 |
10031-10038 |
JJ |
denotes |
lateral |
T12860 |
10049-10050 |
-LRB- |
denotes |
( |
T12861 |
10050-10051 |
NN |
denotes |
a |
T12862 |
10051-10052 |
-RRB- |
denotes |
) |
T12863 |
10053-10056 |
VBP |
denotes |
are |
T12864 |
10057-10065 |
RB |
denotes |
strongly |
T12865 |
10073-10074 |
: |
denotes |
; |
T12866 |
10075-10081 |
NN |
denotes |
square |
T12867 |
10081-10083 |
, |
denotes |
, |
T12868 |
10083-10084 |
DT |
denotes |
a |
T12869 |
10092-10105 |
NN |
denotes |
magnification |
T12870 |
10085-10091 |
JJR |
denotes |
higher |
T12871 |
10106-10108 |
IN |
denotes |
of |
T12872 |
10109-10113 |
DT |
denotes |
this |
T12873 |
10114-10118 |
NN |
denotes |
area |
T12874 |
10119-10121 |
VBZ |
denotes |
is |
T12875 |
10128-10130 |
IN |
denotes |
in |
T12876 |
10131-10132 |
-LRB- |
denotes |
( |
T12877 |
10132-10133 |
NN |
denotes |
I |
T12878 |
10133-10134 |
-RRB- |
denotes |
) |
T12879 |
10134-10135 |
. |
denotes |
. |
T12880 |
10135-10251 |
sentence |
denotes |
(B) Higher magnification of the earlier stage E13 (H) shows, that the cells are stained in the cytosol (arrowhead). |
T12881 |
10136-10137 |
-LRB- |
denotes |
( |
T12882 |
10137-10138 |
LS |
denotes |
B |
T12883 |
10190-10195 |
VBZ |
denotes |
shows |
T12884 |
10138-10139 |
-RRB- |
denotes |
) |
T12885 |
10140-10146 |
JJR |
denotes |
Higher |
T12886 |
10147-10160 |
NN |
denotes |
magnification |
T12887 |
10161-10163 |
IN |
denotes |
of |
T12888 |
10164-10167 |
DT |
denotes |
the |
T12889 |
10182-10185 |
NN |
denotes |
E13 |
T12890 |
10168-10175 |
JJR |
denotes |
earlier |
T12891 |
10176-10181 |
NN |
denotes |
stage |
T12892 |
10186-10187 |
-LRB- |
denotes |
( |
T12893 |
10187-10188 |
NN |
denotes |
H |
T12894 |
10188-10189 |
-RRB- |
denotes |
) |
T12895 |
10195-10197 |
, |
denotes |
, |
T12896 |
10197-10201 |
IN |
denotes |
that |
T12897 |
10216-10223 |
VBN |
denotes |
stained |
T12898 |
10202-10205 |
DT |
denotes |
the |
T12899 |
10206-10211 |
NNS |
denotes |
cells |
T12900 |
10212-10215 |
VBP |
denotes |
are |
T12901 |
10224-10226 |
IN |
denotes |
in |
T12902 |
10227-10230 |
DT |
denotes |
the |
T12903 |
10231-10238 |
NN |
denotes |
cytosol |
T12904 |
10239-10240 |
-LRB- |
denotes |
( |
T12905 |
10240-10249 |
NN |
denotes |
arrowhead |
T12906 |
10249-10250 |
-RRB- |
denotes |
) |
T12907 |
10250-10251 |
. |
denotes |
. |
T12908 |
10251-10341 |
sentence |
denotes |
(C) Two days later at E15 the cells have rounded up, and Annexin A7 stays in the cytosol. |
T12909 |
10252-10253 |
-LRB- |
denotes |
( |
T12910 |
10253-10254 |
LS |
denotes |
C |
T12911 |
10293-10300 |
VBN |
denotes |
rounded |
T12912 |
10254-10255 |
-RRB- |
denotes |
) |
T12913 |
10256-10259 |
CD |
denotes |
Two |
T12914 |
10260-10264 |
NNS |
denotes |
days |
T12915 |
10265-10270 |
RBR |
denotes |
later |
T12916 |
10271-10273 |
IN |
denotes |
at |
T12917 |
10274-10277 |
NN |
denotes |
E15 |
T12918 |
10278-10281 |
DT |
denotes |
the |
T12919 |
10282-10287 |
NNS |
denotes |
cells |
T12920 |
10288-10292 |
VBP |
denotes |
have |
T12921 |
10301-10303 |
RP |
denotes |
up |
T12922 |
10303-10305 |
, |
denotes |
, |
T12923 |
10305-10308 |
CC |
denotes |
and |
T12924 |
10309-10316 |
NN |
denotes |
Annexin |
T12925 |
10317-10319 |
NN |
denotes |
A7 |
T12926 |
10320-10325 |
VBZ |
denotes |
stays |
T12927 |
10326-10328 |
IN |
denotes |
in |
T12928 |
10329-10332 |
DT |
denotes |
the |
T12929 |
10333-10340 |
NN |
denotes |
cytosol |
T12930 |
10340-10341 |
. |
denotes |
. |
T12931 |
10341-10540 |
sentence |
denotes |
(D) At E16 the first nuclear staining becomes apparent (arrowhead) in cells of the intermediate zone located between ventricular germinative zone and marginal neopallial cortex as seen in (I), oval. |
T12932 |
10342-10343 |
-LRB- |
denotes |
( |
T12933 |
10343-10344 |
LS |
denotes |
D |
T12934 |
10380-10387 |
VBZ |
denotes |
becomes |
T12935 |
10344-10345 |
-RRB- |
denotes |
) |
T12936 |
10346-10348 |
IN |
denotes |
At |
T12937 |
10349-10352 |
NN |
denotes |
E16 |
T12938 |
10353-10356 |
DT |
denotes |
the |
T12939 |
10371-10379 |
NN |
denotes |
staining |
T12940 |
10357-10362 |
JJ |
denotes |
first |
T12941 |
10363-10370 |
JJ |
denotes |
nuclear |
T12942 |
10388-10396 |
JJ |
denotes |
apparent |
T12943 |
10397-10398 |
-LRB- |
denotes |
( |
T12944 |
10398-10407 |
NN |
denotes |
arrowhead |
T12945 |
10407-10408 |
-RRB- |
denotes |
) |
T12946 |
10409-10411 |
IN |
denotes |
in |
T12947 |
10412-10417 |
NNS |
denotes |
cells |
T12948 |
10418-10420 |
IN |
denotes |
of |
T12949 |
10421-10424 |
DT |
denotes |
the |
T12950 |
10438-10442 |
NN |
denotes |
zone |
T12951 |
10425-10437 |
JJ |
denotes |
intermediate |
T12952 |
10443-10450 |
VBN |
denotes |
located |
T12953 |
10451-10458 |
IN |
denotes |
between |
T12954 |
10459-10470 |
JJ |
denotes |
ventricular |
T12955 |
10483-10487 |
NN |
denotes |
zone |
T12956 |
10471-10482 |
JJ |
denotes |
germinative |
T12957 |
10488-10491 |
CC |
denotes |
and |
T12958 |
10492-10500 |
JJ |
denotes |
marginal |
T12959 |
10512-10518 |
NN |
denotes |
cortex |
T12960 |
10501-10511 |
JJ |
denotes |
neopallial |
T12961 |
10519-10521 |
IN |
denotes |
as |
T12962 |
10522-10526 |
VBN |
denotes |
seen |
T12963 |
10527-10529 |
IN |
denotes |
in |
T12964 |
10530-10531 |
-LRB- |
denotes |
( |
T12965 |
10531-10532 |
NN |
denotes |
I |
T12966 |
10532-10533 |
-RRB- |
denotes |
) |
T12967 |
10533-10535 |
, |
denotes |
, |
T12968 |
10535-10539 |
NN |
denotes |
oval |
T12969 |
10539-10540 |
. |
denotes |
. |
T12970 |
10540-10595 |
sentence |
denotes |
(E-G) Confocal microscopy confirms the results in B-D. |
T12971 |
10541-10542 |
-LRB- |
denotes |
( |
T12972 |
10542-10543 |
LS |
denotes |
E |
T12973 |
10567-10575 |
VBZ |
denotes |
confirms |
T12974 |
10543-10544 |
SYM |
denotes |
- |
T12975 |
10544-10545 |
LS |
denotes |
G |
T12976 |
10545-10546 |
-RRB- |
denotes |
) |
T12977 |
10547-10555 |
JJ |
denotes |
Confocal |
T12978 |
10556-10566 |
NN |
denotes |
microscopy |
T12979 |
10576-10579 |
DT |
denotes |
the |
T12980 |
10580-10587 |
NNS |
denotes |
results |
T12981 |
10588-10590 |
IN |
denotes |
in |
T12982 |
10591-10592 |
NN |
denotes |
B |
T12983 |
10592-10593 |
SYM |
denotes |
- |
T12984 |
10593-10594 |
NN |
denotes |
D |
T12985 |
10594-10595 |
. |
denotes |
. |
T12986 |
10595-10693 |
sentence |
denotes |
(H) Overview, at E13 the immature GFAP-negative cells are strongly stained; a, lateral ventricle. |
T12987 |
10596-10597 |
-LRB- |
denotes |
( |
T12988 |
10597-10598 |
LS |
denotes |
H |
T12989 |
10663-10670 |
VBN |
denotes |
stained |
T12990 |
10598-10599 |
-RRB- |
denotes |
) |
T12991 |
10600-10608 |
NN |
denotes |
Overview |
T12992 |
10608-10610 |
, |
denotes |
, |
T12993 |
10610-10612 |
IN |
denotes |
at |
T12994 |
10613-10616 |
NN |
denotes |
E13 |
T12995 |
10617-10620 |
DT |
denotes |
the |
T12996 |
10644-10649 |
NNS |
denotes |
cells |
T12997 |
10621-10629 |
JJ |
denotes |
immature |
T12998 |
10630-10634 |
NN |
denotes |
GFAP |
T12999 |
10635-10643 |
JJ |
denotes |
negative |
T13000 |
10634-10635 |
HYPH |
denotes |
- |
T13001 |
10650-10653 |
VBP |
denotes |
are |
T13002 |
10654-10662 |
RB |
denotes |
strongly |
T13003 |
10670-10671 |
: |
denotes |
; |
T13004 |
10683-10692 |
NN |
denotes |
ventricle |
T13005 |
10672-10673 |
NN |
denotes |
a |
T13006 |
10673-10675 |
, |
denotes |
, |
T13007 |
10675-10682 |
JJ |
denotes |
lateral |
T13008 |
10692-10693 |
. |
denotes |
. |
T13009 |
10693-10877 |
sentence |
denotes |
(I) Higher magnification of a cortical section of (A, square) demonstrating ventricular, intermediate, and marginal zones; oval, a higher magnification of this area is given in (D,G). |
T13010 |
10694-10695 |
-LRB- |
denotes |
( |
T13011 |
10695-10696 |
LS |
denotes |
I |
T13012 |
10705-10718 |
NN |
denotes |
magnification |
T13013 |
10696-10697 |
-RRB- |
denotes |
) |
T13014 |
10698-10704 |
JJR |
denotes |
Higher |
T13015 |
10719-10721 |
IN |
denotes |
of |
T13016 |
10722-10723 |
DT |
denotes |
a |
T13017 |
10733-10740 |
NN |
denotes |
section |
T13018 |
10724-10732 |
JJ |
denotes |
cortical |
T13019 |
10741-10743 |
IN |
denotes |
of |
T13020 |
10744-10745 |
-LRB- |
denotes |
( |
T13021 |
10745-10746 |
NN |
denotes |
A |
T13022 |
10746-10748 |
, |
denotes |
, |
T13023 |
10748-10754 |
NN |
denotes |
square |
T13024 |
10754-10755 |
-RRB- |
denotes |
) |
T13025 |
10756-10769 |
VBG |
denotes |
demonstrating |
T13026 |
10770-10781 |
JJ |
denotes |
ventricular |
T13027 |
10810-10815 |
NNS |
denotes |
zones |
T13028 |
10781-10783 |
, |
denotes |
, |
T13029 |
10783-10795 |
JJ |
denotes |
intermediate |
T13030 |
10795-10797 |
, |
denotes |
, |
T13031 |
10797-10800 |
CC |
denotes |
and |
T13032 |
10801-10809 |
JJ |
denotes |
marginal |
T13033 |
10815-10816 |
: |
denotes |
; |
T13034 |
10817-10821 |
NN |
denotes |
oval |
T13035 |
10862-10867 |
VBN |
denotes |
given |
T13036 |
10821-10823 |
, |
denotes |
, |
T13037 |
10823-10824 |
DT |
denotes |
a |
T13038 |
10832-10845 |
NN |
denotes |
magnification |
T13039 |
10825-10831 |
JJR |
denotes |
higher |
T13040 |
10846-10848 |
IN |
denotes |
of |
T13041 |
10849-10853 |
DT |
denotes |
this |
T13042 |
10854-10858 |
NN |
denotes |
area |
T13043 |
10859-10861 |
VBZ |
denotes |
is |
T13044 |
10868-10870 |
IN |
denotes |
in |
T13045 |
10871-10872 |
-LRB- |
denotes |
( |
T13046 |
10872-10873 |
NN |
denotes |
D |
T13047 |
10874-10875 |
NN |
denotes |
G |
T13048 |
10873-10874 |
, |
denotes |
, |
T13049 |
10875-10876 |
-RRB- |
denotes |
) |
T13050 |
10876-10877 |
. |
denotes |
. |
T13536 |
12155-12162 |
NN |
denotes |
Annexin |
T13537 |
12163-12165 |
NN |
denotes |
A7 |
T13538 |
12166-12168 |
VBZ |
denotes |
is |
T13539 |
12169-12176 |
JJ |
denotes |
present |
T13540 |
12177-12179 |
IN |
denotes |
in |
T13541 |
12180-12187 |
NNS |
denotes |
neurons |
T13542 |
12188-12191 |
CC |
denotes |
and |
T13543 |
12192-12202 |
NNS |
denotes |
astrocytes |
T13544 |
12203-12205 |
IN |
denotes |
of |
T13545 |
12206-12209 |
DT |
denotes |
the |
T13546 |
12217-12227 |
NN |
denotes |
temporalis |
T13547 |
12210-12216 |
NN |
denotes |
cortex |
T13548 |
12228-12231 |
CC |
denotes |
and |
T13549 |
12232-12243 |
JJ |
denotes |
hippocampal |
T13550 |
12244-12253 |
NN |
denotes |
formation |
T13551 |
12254-12256 |
IN |
denotes |
of |
T13552 |
12257-12259 |
CD |
denotes |
10 |
T13553 |
12260-12265 |
NNS |
denotes |
weeks |
T13554 |
12259-12260 |
HYPH |
denotes |
- |
T13555 |
12266-12269 |
JJ |
denotes |
old |
T13556 |
12265-12266 |
HYPH |
denotes |
- |
T13557 |
12270-12274 |
NNS |
denotes |
mice |
T13558 |
12274-12275 |
. |
denotes |
. |
T13559 |
12275-12476 |
sentence |
denotes |
(A) Low magnification of the cortex temporalis presents an Annexin A7 expression in cells of the pial border, in neurons of all six isocortical laminae, and a weak signal in the adjacent white matter. |
T13560 |
12276-12277 |
-LRB- |
denotes |
( |
T13561 |
12277-12278 |
LS |
denotes |
A |
T13562 |
12323-12331 |
VBZ |
denotes |
presents |
T13563 |
12278-12279 |
-RRB- |
denotes |
) |
T13564 |
12280-12283 |
JJ |
denotes |
Low |
T13565 |
12284-12297 |
NN |
denotes |
magnification |
T13566 |
12298-12300 |
IN |
denotes |
of |
T13567 |
12301-12304 |
DT |
denotes |
the |
T13568 |
12312-12322 |
NN |
denotes |
temporalis |
T13569 |
12305-12311 |
NN |
denotes |
cortex |
T13570 |
12332-12334 |
DT |
denotes |
an |
T13571 |
12346-12356 |
NN |
denotes |
expression |
T13572 |
12335-12342 |
NN |
denotes |
Annexin |
T13573 |
12343-12345 |
NN |
denotes |
A7 |
T13574 |
12357-12359 |
IN |
denotes |
in |
T13575 |
12360-12365 |
NNS |
denotes |
cells |
T13576 |
12366-12368 |
IN |
denotes |
of |
T13577 |
12369-12372 |
DT |
denotes |
the |
T13578 |
12378-12384 |
NN |
denotes |
border |
T13579 |
12373-12377 |
JJ |
denotes |
pial |
T13580 |
12384-12386 |
, |
denotes |
, |
T13581 |
12386-12388 |
IN |
denotes |
in |
T13582 |
12389-12396 |
NNS |
denotes |
neurons |
T13583 |
12397-12399 |
IN |
denotes |
of |
T13584 |
12400-12403 |
DT |
denotes |
all |
T13585 |
12420-12427 |
NNS |
denotes |
laminae |
T13586 |
12404-12407 |
CD |
denotes |
six |
T13587 |
12408-12419 |
JJ |
denotes |
isocortical |
T13588 |
12427-12429 |
, |
denotes |
, |
T13589 |
12429-12432 |
CC |
denotes |
and |
T13590 |
12433-12434 |
DT |
denotes |
a |
T13591 |
12440-12446 |
NN |
denotes |
signal |
T13592 |
12435-12439 |
JJ |
denotes |
weak |
T13593 |
12447-12449 |
IN |
denotes |
in |
T13594 |
12450-12453 |
DT |
denotes |
the |
T13595 |
12469-12475 |
NN |
denotes |
matter |
T13596 |
12454-12462 |
JJ |
denotes |
adjacent |
T13597 |
12463-12468 |
JJ |
denotes |
white |
T13598 |
12475-12476 |
. |
denotes |
. |
T13599 |
12476-12521 |
sentence |
denotes |
(B) Corresponding section stained with GFAP. |
T13600 |
12477-12478 |
-LRB- |
denotes |
( |
T13601 |
12478-12479 |
LS |
denotes |
B |
T13602 |
12495-12502 |
NN |
denotes |
section |
T13603 |
12479-12480 |
-RRB- |
denotes |
) |
T13604 |
12481-12494 |
JJ |
denotes |
Corresponding |
T13605 |
12503-12510 |
VBD |
denotes |
stained |
T13606 |
12511-12515 |
IN |
denotes |
with |
T13607 |
12516-12520 |
NN |
denotes |
GFAP |
T13608 |
12520-12521 |
. |
denotes |
. |
T13609 |
12521-12683 |
sentence |
denotes |
(C) Staining in the Stratum pyramidalis (a) and in the dentate gyrus of the hippocampus; square, a higher magnification of acorresponding area is given in (G,H). |
T13610 |
12522-12523 |
-LRB- |
denotes |
( |
T13611 |
12523-12524 |
LS |
denotes |
C |
T13612 |
12526-12534 |
NN |
denotes |
Staining |
T13613 |
12524-12525 |
-RRB- |
denotes |
) |
T13614 |
12535-12537 |
IN |
denotes |
in |
T13615 |
12538-12541 |
DT |
denotes |
the |
T13616 |
12550-12561 |
NN |
denotes |
pyramidalis |
T13617 |
12542-12549 |
NN |
denotes |
Stratum |
T13618 |
12562-12563 |
-LRB- |
denotes |
( |
T13619 |
12563-12564 |
NN |
denotes |
a |
T13620 |
12564-12565 |
-RRB- |
denotes |
) |
T13621 |
12566-12569 |
CC |
denotes |
and |
T13622 |
12570-12572 |
IN |
denotes |
in |
T13623 |
12573-12576 |
DT |
denotes |
the |
T13624 |
12585-12590 |
NN |
denotes |
gyrus |
T13625 |
12577-12584 |
NN |
denotes |
dentate |
T13626 |
12591-12593 |
IN |
denotes |
of |
T13627 |
12594-12597 |
DT |
denotes |
the |
T13628 |
12598-12609 |
NN |
denotes |
hippocampus |
T13629 |
12609-12610 |
: |
denotes |
; |
T13630 |
12611-12617 |
NN |
denotes |
square |
T13631 |
12668-12673 |
VBN |
denotes |
given |
T13632 |
12617-12619 |
, |
denotes |
, |
T13633 |
12619-12620 |
DT |
denotes |
a |
T13634 |
12628-12641 |
NN |
denotes |
magnification |
T13635 |
12621-12627 |
JJR |
denotes |
higher |
T13636 |
12642-12644 |
IN |
denotes |
of |
T13637 |
12645-12659 |
JJ |
denotes |
acorresponding |
T13638 |
12660-12664 |
NN |
denotes |
area |
T13639 |
12665-12667 |
VBZ |
denotes |
is |
T13640 |
12674-12676 |
IN |
denotes |
in |
T13641 |
12677-12678 |
-LRB- |
denotes |
( |
T13642 |
12678-12679 |
NN |
denotes |
G |
T13643 |
12680-12681 |
NN |
denotes |
H |
T13644 |
12679-12680 |
, |
denotes |
, |
T13645 |
12681-12682 |
-RRB- |
denotes |
) |
T13646 |
12682-12683 |
. |
denotes |
. |
T13647 |
12683-12735 |
sentence |
denotes |
An intense Annexin A7 immunostaining is detectable. |
T13648 |
12684-12686 |
DT |
denotes |
An |
T13649 |
12706-12720 |
NN |
denotes |
immunostaining |
T13650 |
12687-12694 |
JJ |
denotes |
intense |
T13651 |
12695-12702 |
NN |
denotes |
Annexin |
T13652 |
12703-12705 |
NN |
denotes |
A7 |
T13653 |
12721-12723 |
VBZ |
denotes |
is |
T13654 |
12724-12734 |
JJ |
denotes |
detectable |
T13655 |
12734-12735 |
. |
denotes |
. |
T13656 |
12735-12799 |
sentence |
denotes |
(D) Corresponding section stained with secondary antibody only. |
T13657 |
12736-12737 |
-LRB- |
denotes |
( |
T13658 |
12737-12738 |
LS |
denotes |
D |
T13659 |
12754-12761 |
NN |
denotes |
section |
T13660 |
12738-12739 |
-RRB- |
denotes |
) |
T13661 |
12740-12753 |
JJ |
denotes |
Corresponding |
T13662 |
12762-12769 |
VBN |
denotes |
stained |
T13663 |
12770-12774 |
IN |
denotes |
with |
T13664 |
12775-12784 |
JJ |
denotes |
secondary |
T13665 |
12785-12793 |
NN |
denotes |
antibody |
T13666 |
12794-12798 |
RB |
denotes |
only |
T13667 |
12798-12799 |
. |
denotes |
. |
T13668 |
12799-12905 |
sentence |
denotes |
(E) Presence of Annexin A7 in pyramidal neurons (lamina pyramidalis externa) of the isocortex temporalis. |
T13669 |
12800-12801 |
-LRB- |
denotes |
( |
T13670 |
12801-12802 |
LS |
denotes |
E |
T13671 |
12804-12812 |
NN |
denotes |
Presence |
T13672 |
12802-12803 |
-RRB- |
denotes |
) |
T13673 |
12813-12815 |
IN |
denotes |
of |
T13674 |
12816-12823 |
NN |
denotes |
Annexin |
T13675 |
12824-12826 |
NN |
denotes |
A7 |
T13676 |
12827-12829 |
IN |
denotes |
in |
T13677 |
12830-12839 |
JJ |
denotes |
pyramidal |
T13678 |
12840-12847 |
NNS |
denotes |
neurons |
T13679 |
12848-12849 |
-LRB- |
denotes |
( |
T13680 |
12868-12875 |
NN |
denotes |
externa |
T13681 |
12849-12855 |
NN |
denotes |
lamina |
T13682 |
12856-12867 |
NN |
denotes |
pyramidalis |
T13683 |
12875-12876 |
-RRB- |
denotes |
) |
T13684 |
12877-12879 |
IN |
denotes |
of |
T13685 |
12880-12883 |
DT |
denotes |
the |
T13686 |
12894-12904 |
NN |
denotes |
temporalis |
T13687 |
12884-12893 |
NN |
denotes |
isocortex |
T13688 |
12904-12905 |
. |
denotes |
. |
T13689 |
12905-13002 |
sentence |
denotes |
These neurons were identified based on their morphology, distribution and lack of GFAP staining. |
T13690 |
12906-12911 |
DT |
denotes |
These |
T13691 |
12912-12919 |
NNS |
denotes |
neurons |
T13692 |
12925-12935 |
VBN |
denotes |
identified |
T13693 |
12920-12924 |
VBD |
denotes |
were |
T13694 |
12936-12941 |
VBN |
denotes |
based |
T13695 |
12942-12944 |
IN |
denotes |
on |
T13696 |
12945-12950 |
PRP$ |
denotes |
their |
T13697 |
12951-12961 |
NN |
denotes |
morphology |
T13698 |
12961-12963 |
, |
denotes |
, |
T13699 |
12963-12975 |
NN |
denotes |
distribution |
T13700 |
12976-12979 |
CC |
denotes |
and |
T13701 |
12980-12984 |
NN |
denotes |
lack |
T13702 |
12985-12987 |
IN |
denotes |
of |
T13703 |
12988-12992 |
NN |
denotes |
GFAP |
T13704 |
12993-13001 |
NN |
denotes |
staining |
T13705 |
13001-13002 |
. |
denotes |
. |
T13706 |
13002-13090 |
sentence |
denotes |
AnnexinA7 exhibits a punctate staining, which is pronounced in the nucleus (arrowhead). |
T13707 |
13003-13012 |
NN |
denotes |
AnnexinA7 |
T13708 |
13013-13021 |
VBZ |
denotes |
exhibits |
T13709 |
13022-13023 |
DT |
denotes |
a |
T13710 |
13033-13041 |
NN |
denotes |
staining |
T13711 |
13024-13032 |
NN |
denotes |
punctate |
T13712 |
13041-13043 |
, |
denotes |
, |
T13713 |
13043-13048 |
WDT |
denotes |
which |
T13714 |
13049-13051 |
VBZ |
denotes |
is |
T13715 |
13052-13062 |
JJ |
denotes |
pronounced |
T13716 |
13063-13065 |
IN |
denotes |
in |
T13717 |
13066-13069 |
DT |
denotes |
the |
T13718 |
13070-13077 |
NN |
denotes |
nucleus |
T13719 |
13078-13079 |
-LRB- |
denotes |
( |
T13720 |
13079-13088 |
NN |
denotes |
arrowhead |
T13721 |
13088-13089 |
-RRB- |
denotes |
) |
T13722 |
13089-13090 |
. |
denotes |
. |
T13723 |
13090-13317 |
sentence |
denotes |
(F) Higher magnification of image (A) also shows Annexin A7 in nuclei of neurons (lamina granularis externa (corpuscularis), arrowhead) and in the cytoplasm and nuclei of astrocytes (lamina molecularis, arrow; GFAP-confirmed). |
T13724 |
13091-13092 |
-LRB- |
denotes |
( |
T13725 |
13092-13093 |
LS |
denotes |
F |
T13726 |
13134-13139 |
VBZ |
denotes |
shows |
T13727 |
13093-13094 |
-RRB- |
denotes |
) |
T13728 |
13095-13101 |
JJR |
denotes |
Higher |
T13729 |
13102-13115 |
NN |
denotes |
magnification |
T13730 |
13116-13118 |
IN |
denotes |
of |
T13731 |
13119-13124 |
NN |
denotes |
image |
T13732 |
13125-13126 |
-LRB- |
denotes |
( |
T13733 |
13126-13127 |
NN |
denotes |
A |
T13734 |
13127-13128 |
-RRB- |
denotes |
) |
T13735 |
13129-13133 |
RB |
denotes |
also |
T13736 |
13140-13147 |
NN |
denotes |
Annexin |
T13737 |
13148-13150 |
NN |
denotes |
A7 |
T13738 |
13151-13153 |
IN |
denotes |
in |
T13739 |
13154-13160 |
NNS |
denotes |
nuclei |
T13740 |
13161-13163 |
IN |
denotes |
of |
T13741 |
13164-13171 |
NNS |
denotes |
neurons |
T13742 |
13172-13173 |
-LRB- |
denotes |
( |
T13743 |
13216-13225 |
NN |
denotes |
arrowhead |
T13744 |
13173-13179 |
NN |
denotes |
lamina |
T13745 |
13191-13198 |
NN |
denotes |
externa |
T13746 |
13180-13190 |
NN |
denotes |
granularis |
T13747 |
13199-13200 |
-LRB- |
denotes |
( |
T13748 |
13200-13213 |
NN |
denotes |
corpuscularis |
T13749 |
13213-13214 |
-RRB- |
denotes |
) |
T13750 |
13214-13216 |
, |
denotes |
, |
T13751 |
13225-13226 |
-RRB- |
denotes |
) |
T13752 |
13227-13230 |
CC |
denotes |
and |
T13753 |
13231-13233 |
IN |
denotes |
in |
T13754 |
13234-13237 |
DT |
denotes |
the |
T13755 |
13238-13247 |
NN |
denotes |
cytoplasm |
T13756 |
13248-13251 |
CC |
denotes |
and |
T13757 |
13252-13258 |
NNS |
denotes |
nuclei |
T13758 |
13259-13261 |
IN |
denotes |
of |
T13759 |
13262-13272 |
NNS |
denotes |
astrocytes |
T13760 |
13273-13274 |
-LRB- |
denotes |
( |
T13761 |
13294-13299 |
NN |
denotes |
arrow |
T13762 |
13274-13280 |
NN |
denotes |
lamina |
T13763 |
13281-13292 |
NN |
denotes |
molecularis |
T13764 |
13292-13294 |
, |
denotes |
, |
T13765 |
13299-13300 |
: |
denotes |
; |
T13766 |
13301-13305 |
NN |
denotes |
GFAP |
T13767 |
13306-13315 |
VBN |
denotes |
confirmed |
T13768 |
13305-13306 |
HYPH |
denotes |
- |
T13769 |
13315-13316 |
-RRB- |
denotes |
) |
T13770 |
13316-13317 |
. |
denotes |
. |
T13771 |
13317-13480 |
sentence |
denotes |
(G) Higher magnification of the pyramidal neurons in the hippocampus confirms the presence of the Annexin A7 protein in the nucleus (arrowhead) of mature neurons. |
T13772 |
13318-13319 |
-LRB- |
denotes |
( |
T13773 |
13319-13320 |
LS |
denotes |
G |
T13774 |
13387-13395 |
VBZ |
denotes |
confirms |
T13775 |
13320-13321 |
-RRB- |
denotes |
) |
T13776 |
13322-13328 |
JJR |
denotes |
Higher |
T13777 |
13329-13342 |
NN |
denotes |
magnification |
T13778 |
13343-13345 |
IN |
denotes |
of |
T13779 |
13346-13349 |
DT |
denotes |
the |
T13780 |
13360-13367 |
NNS |
denotes |
neurons |
T13781 |
13350-13359 |
JJ |
denotes |
pyramidal |
T13782 |
13368-13370 |
IN |
denotes |
in |
T13783 |
13371-13374 |
DT |
denotes |
the |
T13784 |
13375-13386 |
NN |
denotes |
hippocampus |
T13785 |
13396-13399 |
DT |
denotes |
the |
T13786 |
13400-13408 |
NN |
denotes |
presence |
T13787 |
13409-13411 |
IN |
denotes |
of |
T13788 |
13412-13415 |
DT |
denotes |
the |
T13789 |
13427-13434 |
NN |
denotes |
protein |
T13790 |
13416-13423 |
NN |
denotes |
Annexin |
T13791 |
13424-13426 |
NN |
denotes |
A7 |
T13792 |
13435-13437 |
IN |
denotes |
in |
T13793 |
13438-13441 |
DT |
denotes |
the |
T13794 |
13442-13449 |
NN |
denotes |
nucleus |
T13795 |
13450-13451 |
-LRB- |
denotes |
( |
T13796 |
13451-13460 |
NN |
denotes |
arrowhead |
T13797 |
13460-13461 |
-RRB- |
denotes |
) |
T13798 |
13462-13464 |
IN |
denotes |
of |
T13799 |
13465-13471 |
JJ |
denotes |
mature |
T13800 |
13472-13479 |
NNS |
denotes |
neurons |
T13801 |
13479-13480 |
. |
denotes |
. |
T13802 |
13480-13636 |
sentence |
denotes |
(H) To further confirm this, a similar section derived from an AnxA7-/- mouse was stained with the annexin specific antibody and lacked the nuclear signal. |
T13803 |
13481-13482 |
-LRB- |
denotes |
( |
T13804 |
13482-13483 |
LS |
denotes |
H |
T13805 |
13563-13570 |
VBN |
denotes |
stained |
T13806 |
13483-13484 |
-RRB- |
denotes |
) |
T13807 |
13485-13487 |
TO |
denotes |
To |
T13808 |
13496-13503 |
VB |
denotes |
confirm |
T13809 |
13488-13495 |
RB |
denotes |
further |
T13810 |
13504-13508 |
DT |
denotes |
this |
T13811 |
13508-13510 |
, |
denotes |
, |
T13812 |
13510-13511 |
DT |
denotes |
a |
T13813 |
13520-13527 |
NN |
denotes |
section |
T13814 |
13512-13519 |
JJ |
denotes |
similar |
T13815 |
13528-13535 |
VBN |
denotes |
derived |
T13816 |
13536-13540 |
IN |
denotes |
from |
T13817 |
13541-13543 |
DT |
denotes |
an |
T13818 |
13553-13558 |
NN |
denotes |
mouse |
T13819 |
13544-13549 |
NN |
denotes |
AnxA7 |
T13820 |
13549-13550 |
SYM |
denotes |
- |
T13821 |
13550-13551 |
HYPH |
denotes |
/ |
T13822 |
13551-13552 |
SYM |
denotes |
- |
T13823 |
13559-13562 |
VBD |
denotes |
was |
T13824 |
13571-13575 |
IN |
denotes |
with |
T13825 |
13576-13579 |
DT |
denotes |
the |
T13826 |
13597-13605 |
NN |
denotes |
antibody |
T13827 |
13580-13587 |
NN |
denotes |
annexin |
T13828 |
13588-13596 |
JJ |
denotes |
specific |
T13829 |
13606-13609 |
CC |
denotes |
and |
T13830 |
13610-13616 |
VBD |
denotes |
lacked |
T13831 |
13617-13620 |
DT |
denotes |
the |
T13832 |
13629-13635 |
NN |
denotes |
signal |
T13833 |
13621-13628 |
JJ |
denotes |
nuclear |
T13834 |
13635-13636 |
. |
denotes |
. |
T13835 |
13636-13789 |
sentence |
denotes |
The residual stain of the tissue is unspecific, as it is also observed in controls of the AnxA7-/- brain omitting the primary antibody (data not shown). |
T13836 |
13637-13640 |
DT |
denotes |
The |
T13837 |
13650-13655 |
NN |
denotes |
stain |
T13838 |
13641-13649 |
JJ |
denotes |
residual |
T13839 |
13670-13672 |
VBZ |
denotes |
is |
T13840 |
13656-13658 |
IN |
denotes |
of |
T13841 |
13659-13662 |
DT |
denotes |
the |
T13842 |
13663-13669 |
NN |
denotes |
tissue |
T13843 |
13673-13683 |
JJ |
denotes |
unspecific |
T13844 |
13683-13685 |
, |
denotes |
, |
T13845 |
13685-13687 |
IN |
denotes |
as |
T13846 |
13699-13707 |
VBN |
denotes |
observed |
T13847 |
13688-13690 |
PRP |
denotes |
it |
T13848 |
13691-13693 |
VBZ |
denotes |
is |
T13849 |
13694-13698 |
RB |
denotes |
also |
T13850 |
13708-13710 |
IN |
denotes |
in |
T13851 |
13711-13719 |
NNS |
denotes |
controls |
T13852 |
13720-13722 |
IN |
denotes |
of |
T13853 |
13723-13726 |
DT |
denotes |
the |
T13854 |
13736-13741 |
NN |
denotes |
brain |
T13855 |
13727-13732 |
NN |
denotes |
AnxA7 |
T13856 |
13732-13733 |
SYM |
denotes |
- |
T13857 |
13733-13734 |
HYPH |
denotes |
/ |
T13858 |
13734-13735 |
SYM |
denotes |
- |
T13859 |
13742-13750 |
VBG |
denotes |
omitting |
T13860 |
13751-13754 |
DT |
denotes |
the |
T13861 |
13763-13771 |
NN |
denotes |
antibody |
T13862 |
13755-13762 |
JJ |
denotes |
primary |
T13863 |
13772-13773 |
-LRB- |
denotes |
( |
T13864 |
13782-13787 |
VBN |
denotes |
shown |
T13865 |
13773-13777 |
NNS |
denotes |
data |
T13866 |
13778-13781 |
RB |
denotes |
not |
T13867 |
13787-13788 |
-RRB- |
denotes |
) |
T13868 |
13788-13789 |
. |
denotes |
. |
T13869 |
13789-13884 |
sentence |
denotes |
All paraffin sections were stained with mAb 203–217 (A, C, E, F, G) or anti-GFAP-antibody (B). |
T13870 |
13790-13793 |
DT |
denotes |
All |
T13871 |
13803-13811 |
NNS |
denotes |
sections |
T13872 |
13794-13802 |
NN |
denotes |
paraffin |
T13873 |
13817-13824 |
VBN |
denotes |
stained |
T13874 |
13812-13816 |
VBD |
denotes |
were |
T13875 |
13825-13829 |
IN |
denotes |
with |
T13876 |
13830-13833 |
NN |
denotes |
mAb |
T13877 |
13834-13837 |
CD |
denotes |
203 |
T13878 |
13837-13838 |
SYM |
denotes |
– |
T13879 |
13838-13841 |
CD |
denotes |
217 |
T13880 |
13842-13843 |
-LRB- |
denotes |
( |
T13881 |
13855-13856 |
NN |
denotes |
G |
T13882 |
13843-13844 |
NN |
denotes |
A |
T13883 |
13844-13846 |
, |
denotes |
, |
T13884 |
13846-13847 |
NN |
denotes |
C |
T13885 |
13847-13849 |
, |
denotes |
, |
T13886 |
13849-13850 |
NN |
denotes |
E |
T13887 |
13850-13852 |
, |
denotes |
, |
T13888 |
13852-13853 |
NN |
denotes |
F |
T13889 |
13853-13855 |
, |
denotes |
, |
T13890 |
13856-13857 |
-RRB- |
denotes |
) |
T13891 |
13858-13860 |
CC |
denotes |
or |
T13892 |
13861-13870 |
JJ |
denotes |
anti-GFAP |
T13893 |
13871-13879 |
NN |
denotes |
antibody |
T13894 |
13870-13871 |
HYPH |
denotes |
- |
T13895 |
13880-13881 |
-LRB- |
denotes |
( |
T13896 |
13881-13882 |
NN |
denotes |
B |
T13897 |
13882-13883 |
-RRB- |
denotes |
) |
T13898 |
13883-13884 |
. |
denotes |
. |
T13899 |
13884-13948 |
sentence |
denotes |
The hippocampal control section (D) lacks the primary antibody. |
T13900 |
13885-13888 |
DT |
denotes |
The |
T13901 |
13909-13916 |
NN |
denotes |
section |
T13902 |
13889-13900 |
JJ |
denotes |
hippocampal |
T13903 |
13901-13908 |
NN |
denotes |
control |
T13904 |
13921-13926 |
VBZ |
denotes |
lacks |
T13905 |
13917-13918 |
-LRB- |
denotes |
( |
T13906 |
13918-13919 |
NN |
denotes |
D |
T13907 |
13919-13920 |
-RRB- |
denotes |
) |
T13908 |
13927-13930 |
DT |
denotes |
the |
T13909 |
13939-13947 |
NN |
denotes |
antibody |
T13910 |
13931-13938 |
JJ |
denotes |
primary |
T13911 |
13947-13948 |
. |
denotes |
. |
T14188 |
16550-16557 |
NN |
denotes |
Annexin |
T14189 |
16558-16560 |
NN |
denotes |
A7 |
T14190 |
16561-16575 |
NN |
denotes |
immunostaining |
T14191 |
16576-16578 |
IN |
denotes |
in |
T14192 |
16579-16582 |
DT |
denotes |
the |
T14193 |
16583-16593 |
NN |
denotes |
cerebellum |
T14194 |
16594-16596 |
IN |
denotes |
of |
T14195 |
16597-16602 |
JJ |
denotes |
adult |
T14196 |
16603-16607 |
NNS |
denotes |
mice |
T14197 |
16607-16608 |
. |
denotes |
. |
T14198 |
16608-16748 |
sentence |
denotes |
(A) Low magnification of the cerebellum presents an Annexin A7 expression mainly in cells of the stratum granulosum and laminae medullares. |
T14199 |
16609-16610 |
-LRB- |
denotes |
( |
T14200 |
16610-16611 |
LS |
denotes |
A |
T14201 |
16649-16657 |
VBZ |
denotes |
presents |
T14202 |
16611-16612 |
-RRB- |
denotes |
) |
T14203 |
16613-16616 |
JJ |
denotes |
Low |
T14204 |
16617-16630 |
NN |
denotes |
magnification |
T14205 |
16631-16633 |
IN |
denotes |
of |
T14206 |
16634-16637 |
DT |
denotes |
the |
T14207 |
16638-16648 |
NN |
denotes |
cerebellum |
T14208 |
16658-16660 |
DT |
denotes |
an |
T14209 |
16672-16682 |
NN |
denotes |
expression |
T14210 |
16661-16668 |
NN |
denotes |
Annexin |
T14211 |
16669-16671 |
NN |
denotes |
A7 |
T14212 |
16683-16689 |
RB |
denotes |
mainly |
T14213 |
16690-16692 |
IN |
denotes |
in |
T14214 |
16693-16698 |
NNS |
denotes |
cells |
T14215 |
16699-16701 |
IN |
denotes |
of |
T14216 |
16702-16705 |
DT |
denotes |
the |
T14217 |
16714-16724 |
NN |
denotes |
granulosum |
T14218 |
16706-16713 |
NN |
denotes |
stratum |
T14219 |
16725-16728 |
CC |
denotes |
and |
T14220 |
16729-16736 |
NNS |
denotes |
laminae |
T14221 |
16737-16747 |
NNS |
denotes |
medullares |
T14222 |
16747-16748 |
. |
denotes |
. |
T14223 |
16748-16792 |
sentence |
denotes |
(B) Corresponding section stained for GFAP. |
T14224 |
16749-16750 |
-LRB- |
denotes |
( |
T14225 |
16750-16751 |
LS |
denotes |
B |
T14226 |
16767-16774 |
NN |
denotes |
section |
T14227 |
16751-16752 |
-RRB- |
denotes |
) |
T14228 |
16753-16766 |
JJ |
denotes |
Corresponding |
T14229 |
16775-16782 |
VBN |
denotes |
stained |
T14230 |
16783-16786 |
IN |
denotes |
for |
T14231 |
16787-16791 |
NN |
denotes |
GFAP |
T14232 |
16791-16792 |
. |
denotes |
. |
T14233 |
16792-16994 |
sentence |
denotes |
(C) Higher magnification of folia of the cerebellum, where a polyclonal anti-AnnexinA7 antibody was used; square, a higher magnification of acorresponding area stained with mAb 203–217 is given in (D). |
T14234 |
16793-16794 |
-LRB- |
denotes |
( |
T14235 |
16794-16795 |
LS |
denotes |
C |
T14236 |
16804-16817 |
NN |
denotes |
magnification |
T14237 |
16795-16796 |
-RRB- |
denotes |
) |
T14238 |
16797-16803 |
JJR |
denotes |
Higher |
T14239 |
16818-16820 |
IN |
denotes |
of |
T14240 |
16821-16826 |
NNS |
denotes |
folia |
T14241 |
16827-16829 |
IN |
denotes |
of |
T14242 |
16830-16833 |
DT |
denotes |
the |
T14243 |
16834-16844 |
NN |
denotes |
cerebellum |
T14244 |
16844-16846 |
, |
denotes |
, |
T14245 |
16846-16851 |
WRB |
denotes |
where |
T14246 |
16893-16897 |
VBN |
denotes |
used |
T14247 |
16852-16853 |
DT |
denotes |
a |
T14248 |
16880-16888 |
NN |
denotes |
antibody |
T14249 |
16854-16864 |
JJ |
denotes |
polyclonal |
T14250 |
16865-16879 |
JJ |
denotes |
anti-AnnexinA7 |
T14251 |
16889-16892 |
VBD |
denotes |
was |
T14252 |
16897-16898 |
: |
denotes |
; |
T14253 |
16899-16905 |
NN |
denotes |
square |
T14254 |
16981-16986 |
VBN |
denotes |
given |
T14255 |
16905-16907 |
, |
denotes |
, |
T14256 |
16907-16908 |
DT |
denotes |
a |
T14257 |
16916-16929 |
NN |
denotes |
magnification |
T14258 |
16909-16915 |
JJR |
denotes |
higher |
T14259 |
16930-16932 |
IN |
denotes |
of |
T14260 |
16933-16947 |
JJ |
denotes |
acorresponding |
T14261 |
16948-16952 |
NN |
denotes |
area |
T14262 |
16953-16960 |
VBN |
denotes |
stained |
T14263 |
16961-16965 |
IN |
denotes |
with |
T14264 |
16966-16969 |
NN |
denotes |
mAb |
T14265 |
16970-16973 |
CD |
denotes |
203 |
T14266 |
16973-16974 |
SYM |
denotes |
– |
T14267 |
16974-16977 |
CD |
denotes |
217 |
T14268 |
16978-16980 |
VBZ |
denotes |
is |
T14269 |
16987-16989 |
IN |
denotes |
in |
T14270 |
16990-16991 |
-LRB- |
denotes |
( |
T14271 |
16991-16992 |
NN |
denotes |
D |
T14272 |
16992-16993 |
-RRB- |
denotes |
) |
T14273 |
16993-16994 |
. |
denotes |
. |
T14274 |
16994-17136 |
sentence |
denotes |
Between stratum granulosum and stratum moleculare the layer of Purkinje-cells (stratum neuronorum piriformium (ganglionare)) can be observed. |
T14275 |
16995-17002 |
IN |
denotes |
Between |
T14276 |
17127-17135 |
VBN |
denotes |
observed |
T14277 |
17003-17010 |
NN |
denotes |
stratum |
T14278 |
17011-17021 |
NN |
denotes |
granulosum |
T14279 |
17022-17025 |
CC |
denotes |
and |
T14280 |
17026-17033 |
NN |
denotes |
stratum |
T14281 |
17034-17044 |
NN |
denotes |
moleculare |
T14282 |
17045-17048 |
DT |
denotes |
the |
T14283 |
17049-17054 |
NN |
denotes |
layer |
T14284 |
17055-17057 |
IN |
denotes |
of |
T14285 |
17058-17066 |
NNP |
denotes |
Purkinje |
T14286 |
17067-17072 |
NNS |
denotes |
cells |
T14287 |
17066-17067 |
HYPH |
denotes |
- |
T14288 |
17073-17074 |
-LRB- |
denotes |
( |
T14289 |
17093-17104 |
NN |
denotes |
piriformium |
T14290 |
17074-17081 |
NN |
denotes |
stratum |
T14291 |
17082-17092 |
NN |
denotes |
neuronorum |
T14292 |
17105-17106 |
-LRB- |
denotes |
( |
T14293 |
17106-17117 |
NN |
denotes |
ganglionare |
T14294 |
17117-17118 |
-RRB- |
denotes |
) |
T14295 |
17118-17119 |
-RRB- |
denotes |
) |
T14296 |
17120-17123 |
MD |
denotes |
can |
T14297 |
17124-17126 |
VB |
denotes |
be |
T14298 |
17135-17136 |
. |
denotes |
. |
T14299 |
17136-17189 |
sentence |
denotes |
(D) Staining of the band of Purkinje-cells (arrows). |
T14300 |
17137-17138 |
-LRB- |
denotes |
( |
T14301 |
17138-17139 |
LS |
denotes |
D |
T14302 |
17141-17149 |
NN |
denotes |
Staining |
T14303 |
17139-17140 |
-RRB- |
denotes |
) |
T14304 |
17150-17152 |
IN |
denotes |
of |
T14305 |
17153-17156 |
DT |
denotes |
the |
T14306 |
17157-17161 |
NN |
denotes |
band |
T14307 |
17162-17164 |
IN |
denotes |
of |
T14308 |
17165-17173 |
NNP |
denotes |
Purkinje |
T14309 |
17174-17179 |
NNS |
denotes |
cells |
T14310 |
17173-17174 |
HYPH |
denotes |
- |
T14311 |
17180-17181 |
-LRB- |
denotes |
( |
T14312 |
17181-17187 |
NNS |
denotes |
arrows |
T14313 |
17187-17188 |
-RRB- |
denotes |
) |
T14314 |
17188-17189 |
. |
denotes |
. |
T14315 |
17189-17290 |
sentence |
denotes |
The positive Annexin A7-stain in the stratum granulosum is due to staining of the nuclei of neurons. |
T14316 |
17190-17193 |
DT |
denotes |
The |
T14317 |
17214-17219 |
NN |
denotes |
stain |
T14318 |
17194-17202 |
JJ |
denotes |
positive |
T14319 |
17203-17210 |
NN |
denotes |
Annexin |
T14320 |
17211-17213 |
NN |
denotes |
A7 |
T14321 |
17213-17214 |
HYPH |
denotes |
- |
T14322 |
17246-17248 |
VBZ |
denotes |
is |
T14323 |
17220-17222 |
IN |
denotes |
in |
T14324 |
17223-17226 |
DT |
denotes |
the |
T14325 |
17235-17245 |
NN |
denotes |
granulosum |
T14326 |
17227-17234 |
NN |
denotes |
stratum |
T14327 |
17249-17252 |
IN |
denotes |
due |
T14328 |
17253-17255 |
IN |
denotes |
to |
T14329 |
17256-17264 |
NN |
denotes |
staining |
T14330 |
17265-17267 |
IN |
denotes |
of |
T14331 |
17268-17271 |
DT |
denotes |
the |
T14332 |
17272-17278 |
NNS |
denotes |
nuclei |
T14333 |
17279-17281 |
IN |
denotes |
of |
T14334 |
17282-17289 |
NNS |
denotes |
neurons |
T14335 |
17289-17290 |
. |
denotes |
. |
T14336 |
17290-17434 |
sentence |
denotes |
(E) In addition to an intense staining of the nucleus, the cell body is AnnexinA7-positive including both dendrites of the Purkinje-cell shown. |
T14337 |
17291-17292 |
-LRB- |
denotes |
( |
T14338 |
17292-17293 |
LS |
denotes |
E |
T14339 |
17360-17362 |
VBZ |
denotes |
is |
T14340 |
17293-17294 |
-RRB- |
denotes |
) |
T14341 |
17295-17297 |
IN |
denotes |
In |
T14342 |
17298-17306 |
NN |
denotes |
addition |
T14343 |
17307-17309 |
IN |
denotes |
to |
T14344 |
17310-17312 |
DT |
denotes |
an |
T14345 |
17321-17329 |
NN |
denotes |
staining |
T14346 |
17313-17320 |
JJ |
denotes |
intense |
T14347 |
17330-17332 |
IN |
denotes |
of |
T14348 |
17333-17336 |
DT |
denotes |
the |
T14349 |
17337-17344 |
NN |
denotes |
nucleus |
T14350 |
17344-17346 |
, |
denotes |
, |
T14351 |
17346-17349 |
DT |
denotes |
the |
T14352 |
17355-17359 |
NN |
denotes |
body |
T14353 |
17350-17354 |
NN |
denotes |
cell |
T14354 |
17363-17372 |
NN |
denotes |
AnnexinA7 |
T14355 |
17373-17381 |
JJ |
denotes |
positive |
T14356 |
17372-17373 |
HYPH |
denotes |
- |
T14357 |
17382-17391 |
VBG |
denotes |
including |
T14358 |
17392-17396 |
DT |
denotes |
both |
T14359 |
17397-17406 |
NNS |
denotes |
dendrites |
T14360 |
17407-17409 |
IN |
denotes |
of |
T14361 |
17410-17413 |
DT |
denotes |
the |
T14362 |
17423-17427 |
NN |
denotes |
cell |
T14363 |
17414-17422 |
NNP |
denotes |
Purkinje |
T14364 |
17422-17423 |
HYPH |
denotes |
- |
T14365 |
17428-17433 |
VBN |
denotes |
shown |
T14366 |
17433-17434 |
. |
denotes |
. |
T14367 |
17434-17484 |
sentence |
denotes |
(F) Corresponding section from an AnxA7-/- mouse. |
T14368 |
17435-17436 |
-LRB- |
denotes |
( |
T14369 |
17436-17437 |
LS |
denotes |
F |
T14370 |
17453-17460 |
NN |
denotes |
section |
T14371 |
17437-17438 |
-RRB- |
denotes |
) |
T14372 |
17439-17452 |
JJ |
denotes |
Corresponding |
T14373 |
17461-17465 |
IN |
denotes |
from |
T14374 |
17466-17468 |
DT |
denotes |
an |
T14375 |
17478-17483 |
NN |
denotes |
mouse |
T14376 |
17469-17474 |
NN |
denotes |
AnxA7 |
T14377 |
17474-17475 |
SYM |
denotes |
- |
T14378 |
17475-17476 |
HYPH |
denotes |
/ |
T14379 |
17476-17477 |
SYM |
denotes |
- |
T14380 |
17483-17484 |
. |
denotes |
. |
T14381 |
17484-17635 |
sentence |
denotes |
(G) AnnexinA7 staining of axons (arrowheads) running from the laminae medullares to the Purkinje-cell layer located in the round end of a convolution. |
T14382 |
17485-17486 |
-LRB- |
denotes |
( |
T14383 |
17486-17487 |
LS |
denotes |
G |
T14384 |
17499-17507 |
NN |
denotes |
staining |
T14385 |
17487-17488 |
-RRB- |
denotes |
) |
T14386 |
17489-17498 |
NN |
denotes |
AnnexinA7 |
T14387 |
17508-17510 |
IN |
denotes |
of |
T14388 |
17511-17516 |
NNS |
denotes |
axons |
T14389 |
17517-17518 |
-LRB- |
denotes |
( |
T14390 |
17518-17528 |
NNS |
denotes |
arrowheads |
T14391 |
17528-17529 |
-RRB- |
denotes |
) |
T14392 |
17530-17537 |
VBG |
denotes |
running |
T14393 |
17538-17542 |
IN |
denotes |
from |
T14394 |
17543-17546 |
DT |
denotes |
the |
T14395 |
17555-17565 |
NNS |
denotes |
medullares |
T14396 |
17547-17554 |
NNS |
denotes |
laminae |
T14397 |
17566-17568 |
IN |
denotes |
to |
T14398 |
17569-17572 |
DT |
denotes |
the |
T14399 |
17587-17592 |
NN |
denotes |
layer |
T14400 |
17573-17581 |
NNP |
denotes |
Purkinje |
T14401 |
17582-17586 |
NN |
denotes |
cell |
T14402 |
17581-17582 |
HYPH |
denotes |
- |
T14403 |
17593-17600 |
VBN |
denotes |
located |
T14404 |
17601-17603 |
IN |
denotes |
in |
T14405 |
17604-17607 |
DT |
denotes |
the |
T14406 |
17614-17617 |
NN |
denotes |
end |
T14407 |
17608-17613 |
JJ |
denotes |
round |
T14408 |
17618-17620 |
IN |
denotes |
of |
T14409 |
17621-17622 |
DT |
denotes |
a |
T14410 |
17623-17634 |
NN |
denotes |
convolution |
T14411 |
17634-17635 |
. |
denotes |
. |
T14412 |
17635-17689 |
sentence |
denotes |
Sections A, D, E, F, G were stained with mAb 203–217. |
T14413 |
17636-17644 |
NNS |
denotes |
Sections |
T14414 |
17657-17658 |
NN |
denotes |
G |
T14415 |
17645-17646 |
NN |
denotes |
A |
T14416 |
17646-17648 |
, |
denotes |
, |
T14417 |
17648-17649 |
NN |
denotes |
D |
T14418 |
17649-17651 |
, |
denotes |
, |
T14419 |
17651-17652 |
NN |
denotes |
E |
T14420 |
17652-17654 |
, |
denotes |
, |
T14421 |
17654-17655 |
NN |
denotes |
F |
T14422 |
17655-17657 |
, |
denotes |
, |
T14423 |
17664-17671 |
VBN |
denotes |
stained |
T14424 |
17659-17663 |
VBD |
denotes |
were |
T14425 |
17672-17676 |
IN |
denotes |
with |
T14426 |
17677-17680 |
NN |
denotes |
mAb |
T14427 |
17681-17684 |
CD |
denotes |
203 |
T14428 |
17684-17685 |
SYM |
denotes |
– |
T14429 |
17685-17688 |
CD |
denotes |
217 |
T14430 |
17688-17689 |
. |
denotes |
. |
T14500 |
18436-18443 |
NN |
denotes |
Annexin |
T14501 |
18444-18446 |
NN |
denotes |
A7 |
T14502 |
18447-18461 |
NN |
denotes |
immunostaining |
T14503 |
18462-18464 |
IN |
denotes |
in |
T14504 |
18465-18470 |
JJ |
denotes |
human |
T14505 |
18471-18480 |
NN |
denotes |
isocortex |
T14506 |
18480-18481 |
. |
denotes |
. |
T14507 |
18481-18640 |
sentence |
denotes |
(A) Pyramidal neurons (bold arrow) and apical dendrites (small arrows) were clearly labeled with the antibody against Annexin A7 in the human parietal cortex. |
T14508 |
18482-18483 |
-LRB- |
denotes |
( |
T14509 |
18483-18484 |
LS |
denotes |
A |
T14510 |
18566-18573 |
VBN |
denotes |
labeled |
T14511 |
18484-18485 |
-RRB- |
denotes |
) |
T14512 |
18486-18495 |
JJ |
denotes |
Pyramidal |
T14513 |
18496-18503 |
NNS |
denotes |
neurons |
T14514 |
18504-18505 |
-LRB- |
denotes |
( |
T14515 |
18510-18515 |
NN |
denotes |
arrow |
T14516 |
18505-18509 |
JJ |
denotes |
bold |
T14517 |
18515-18516 |
-RRB- |
denotes |
) |
T14518 |
18517-18520 |
CC |
denotes |
and |
T14519 |
18521-18527 |
JJ |
denotes |
apical |
T14520 |
18528-18537 |
NNS |
denotes |
dendrites |
T14521 |
18538-18539 |
-LRB- |
denotes |
( |
T14522 |
18545-18551 |
NNS |
denotes |
arrows |
T14523 |
18539-18544 |
JJ |
denotes |
small |
T14524 |
18551-18552 |
-RRB- |
denotes |
) |
T14525 |
18553-18557 |
VBD |
denotes |
were |
T14526 |
18558-18565 |
RB |
denotes |
clearly |
T14527 |
18574-18578 |
IN |
denotes |
with |
T14528 |
18579-18582 |
DT |
denotes |
the |
T14529 |
18583-18591 |
NN |
denotes |
antibody |
T14530 |
18592-18599 |
IN |
denotes |
against |
T14531 |
18600-18607 |
NN |
denotes |
Annexin |
T14532 |
18608-18610 |
NN |
denotes |
A7 |
T14533 |
18611-18613 |
IN |
denotes |
in |
T14534 |
18614-18617 |
DT |
denotes |
the |
T14535 |
18633-18639 |
NN |
denotes |
cortex |
T14536 |
18618-18623 |
JJ |
denotes |
human |
T14537 |
18624-18632 |
JJ |
denotes |
parietal |
T14538 |
18639-18640 |
. |
denotes |
. |
T14539 |
18640-18718 |
sentence |
denotes |
(B) A dendritic staining was also seen in the molecular layer (small arrows). |
T14540 |
18641-18642 |
-LRB- |
denotes |
( |
T14541 |
18642-18643 |
LS |
denotes |
B |
T14542 |
18675-18679 |
VBN |
denotes |
seen |
T14543 |
18643-18644 |
-RRB- |
denotes |
) |
T14544 |
18645-18646 |
DT |
denotes |
A |
T14545 |
18657-18665 |
NN |
denotes |
staining |
T14546 |
18647-18656 |
JJ |
denotes |
dendritic |
T14547 |
18666-18669 |
VBD |
denotes |
was |
T14548 |
18670-18674 |
RB |
denotes |
also |
T14549 |
18680-18682 |
IN |
denotes |
in |
T14550 |
18683-18686 |
DT |
denotes |
the |
T14551 |
18697-18702 |
NN |
denotes |
layer |
T14552 |
18687-18696 |
JJ |
denotes |
molecular |
T14553 |
18703-18704 |
-LRB- |
denotes |
( |
T14554 |
18710-18716 |
NNS |
denotes |
arrows |
T14555 |
18704-18709 |
JJ |
denotes |
small |
T14556 |
18716-18717 |
-RRB- |
denotes |
) |
T14557 |
18717-18718 |
. |
denotes |
. |
T14558 |
18718-18797 |
sentence |
denotes |
In addition the subpial astrocytes were weakly labeled (double headed arrows). |
T14559 |
18719-18721 |
IN |
denotes |
In |
T14560 |
18766-18773 |
VBN |
denotes |
labeled |
T14561 |
18722-18730 |
NN |
denotes |
addition |
T14562 |
18731-18734 |
DT |
denotes |
the |
T14563 |
18743-18753 |
NNS |
denotes |
astrocytes |
T14564 |
18735-18742 |
JJ |
denotes |
subpial |
T14565 |
18754-18758 |
VBD |
denotes |
were |
T14566 |
18759-18765 |
RB |
denotes |
weakly |
T14567 |
18774-18775 |
-LRB- |
denotes |
( |
T14568 |
18789-18795 |
NNS |
denotes |
arrows |
T14569 |
18775-18781 |
JJ |
denotes |
double |
T14570 |
18782-18788 |
JJ |
denotes |
headed |
T14571 |
18795-18796 |
-RRB- |
denotes |
) |
T14572 |
18796-18797 |
. |
denotes |
. |
T14573 |
18797-18890 |
sentence |
denotes |
The staining was cytoplasmic, only few astrocytes showed a nuclear staining as well (inset). |
T14574 |
18798-18801 |
DT |
denotes |
The |
T14575 |
18802-18810 |
NN |
denotes |
staining |
T14576 |
18811-18814 |
VBD |
denotes |
was |
T14577 |
18848-18854 |
VBD |
denotes |
showed |
T14578 |
18815-18826 |
JJ |
denotes |
cytoplasmic |
T14579 |
18826-18828 |
, |
denotes |
, |
T14580 |
18828-18832 |
RB |
denotes |
only |
T14581 |
18837-18847 |
NNS |
denotes |
astrocytes |
T14582 |
18833-18836 |
JJ |
denotes |
few |
T14583 |
18855-18856 |
DT |
denotes |
a |
T14584 |
18865-18873 |
NN |
denotes |
staining |
T14585 |
18857-18864 |
JJ |
denotes |
nuclear |
T14586 |
18874-18876 |
RB |
denotes |
as |
T14587 |
18877-18881 |
RB |
denotes |
well |
T14588 |
18882-18883 |
-LRB- |
denotes |
( |
T14589 |
18883-18888 |
NN |
denotes |
inset |
T14590 |
18888-18889 |
-RRB- |
denotes |
) |
T14591 |
18889-18890 |
. |
denotes |
. |
T14592 |
18890-19026 |
sentence |
denotes |
(C) Enlargement of (A): Annexin A7 was seen at the cell membrane of the perikaryon (bold arrow) and the apical dendrite (small arrows). |
T14593 |
18891-18892 |
-LRB- |
denotes |
( |
T14594 |
18892-18893 |
LS |
denotes |
C |
T14595 |
18930-18934 |
VBN |
denotes |
seen |
T14596 |
18893-18894 |
-RRB- |
denotes |
) |
T14597 |
18895-18906 |
NN |
denotes |
Enlargement |
T14598 |
18907-18909 |
IN |
denotes |
of |
T14599 |
18910-18911 |
-LRB- |
denotes |
( |
T14600 |
18911-18912 |
NN |
denotes |
A |
T14601 |
18912-18913 |
-RRB- |
denotes |
) |
T14602 |
18913-18915 |
: |
denotes |
: |
T14603 |
18915-18922 |
NN |
denotes |
Annexin |
T14604 |
18923-18925 |
NN |
denotes |
A7 |
T14605 |
18926-18929 |
VBD |
denotes |
was |
T14606 |
18935-18937 |
IN |
denotes |
at |
T14607 |
18938-18941 |
DT |
denotes |
the |
T14608 |
18947-18955 |
NN |
denotes |
membrane |
T14609 |
18942-18946 |
NN |
denotes |
cell |
T14610 |
18956-18958 |
IN |
denotes |
of |
T14611 |
18959-18962 |
DT |
denotes |
the |
T14612 |
18963-18973 |
NN |
denotes |
perikaryon |
T14613 |
18974-18975 |
-LRB- |
denotes |
( |
T14614 |
18980-18985 |
NN |
denotes |
arrow |
T14615 |
18975-18979 |
JJ |
denotes |
bold |
T14616 |
18985-18986 |
-RRB- |
denotes |
) |
T14617 |
18987-18990 |
CC |
denotes |
and |
T14618 |
18991-18994 |
DT |
denotes |
the |
T14619 |
19002-19010 |
NN |
denotes |
dendrite |
T14620 |
18995-19001 |
JJ |
denotes |
apical |
T14621 |
19011-19012 |
-LRB- |
denotes |
( |
T14622 |
19018-19024 |
NNS |
denotes |
arrows |
T14623 |
19012-19017 |
JJ |
denotes |
small |
T14624 |
19024-19025 |
-RRB- |
denotes |
) |
T14625 |
19025-19026 |
. |
denotes |
. |
T14626 |
19026-19184 |
sentence |
denotes |
(D) A blank control lacking the primary antibody did not show a specific labeling, but autofluorescent lipofuscin was detectable in the neurons (arrowheads). |
T14627 |
19027-19028 |
-LRB- |
denotes |
( |
T14628 |
19028-19029 |
LS |
denotes |
D |
T14629 |
19084-19088 |
VB |
denotes |
show |
T14630 |
19029-19030 |
-RRB- |
denotes |
) |
T14631 |
19031-19032 |
DT |
denotes |
A |
T14632 |
19039-19046 |
NN |
denotes |
control |
T14633 |
19033-19038 |
JJ |
denotes |
blank |
T14634 |
19047-19054 |
VBG |
denotes |
lacking |
T14635 |
19055-19058 |
DT |
denotes |
the |
T14636 |
19067-19075 |
NN |
denotes |
antibody |
T14637 |
19059-19066 |
JJ |
denotes |
primary |
T14638 |
19076-19079 |
VBD |
denotes |
did |
T14639 |
19080-19083 |
RB |
denotes |
not |
T14640 |
19089-19090 |
DT |
denotes |
a |
T14641 |
19100-19108 |
NN |
denotes |
labeling |
T14642 |
19091-19099 |
JJ |
denotes |
specific |
T14643 |
19108-19110 |
, |
denotes |
, |
T14644 |
19110-19113 |
CC |
denotes |
but |
T14645 |
19114-19129 |
JJ |
denotes |
autofluorescent |
T14646 |
19130-19140 |
NN |
denotes |
lipofuscin |
T14647 |
19141-19144 |
VBD |
denotes |
was |
T14648 |
19145-19155 |
JJ |
denotes |
detectable |
T14649 |
19156-19158 |
IN |
denotes |
in |
T14650 |
19159-19162 |
DT |
denotes |
the |
T14651 |
19163-19170 |
NNS |
denotes |
neurons |
T14652 |
19171-19172 |
-LRB- |
denotes |
( |
T14653 |
19172-19182 |
NNS |
denotes |
arrowheads |
T14654 |
19182-19183 |
-RRB- |
denotes |
) |
T14655 |
19183-19184 |
. |
denotes |
. |
T14656 |
19184-19242 |
sentence |
denotes |
Bar, (A) 70 μm, (B) 50 μm, (B-inset, C) 17 μm, (D) 20 μm. |
T14657 |
19185-19188 |
NN |
denotes |
Bar |
T14658 |
19188-19190 |
, |
denotes |
, |
T14659 |
19190-19191 |
-LRB- |
denotes |
( |
T14660 |
19191-19192 |
LS |
denotes |
A |
T14661 |
19197-19199 |
NN |
denotes |
μm |
T14662 |
19192-19193 |
-RRB- |
denotes |
) |
T14663 |
19194-19196 |
CD |
denotes |
70 |
T14664 |
19199-19201 |
, |
denotes |
, |
T14665 |
19201-19202 |
-LRB- |
denotes |
( |
T14666 |
19202-19203 |
LS |
denotes |
B |
T14667 |
19208-19210 |
NN |
denotes |
μm |
T14668 |
19203-19204 |
-RRB- |
denotes |
) |
T14669 |
19205-19207 |
CD |
denotes |
50 |
T14670 |
19210-19212 |
, |
denotes |
, |
T14671 |
19212-19213 |
-LRB- |
denotes |
( |
T14672 |
19213-19214 |
LS |
denotes |
B |
T14673 |
19228-19230 |
NN |
denotes |
μm |
T14674 |
19214-19215 |
HYPH |
denotes |
- |
T14675 |
19215-19220 |
NN |
denotes |
inset |
T14676 |
19220-19222 |
, |
denotes |
, |
T14677 |
19222-19223 |
LS |
denotes |
C |
T14678 |
19223-19224 |
-RRB- |
denotes |
) |
T14679 |
19225-19227 |
CD |
denotes |
17 |
T14680 |
19230-19232 |
, |
denotes |
, |
T14681 |
19232-19233 |
-LRB- |
denotes |
( |
T14682 |
19233-19234 |
LS |
denotes |
D |
T14683 |
19239-19241 |
NN |
denotes |
μm |
T14684 |
19234-19235 |
-RRB- |
denotes |
) |
T14685 |
19236-19238 |
CD |
denotes |
20 |
T14686 |
19241-19242 |
. |
denotes |
. |
T14995 |
21385-21392 |
JJ |
denotes |
Nuclear |
T14996 |
21393-21405 |
NN |
denotes |
localization |
T14997 |
21406-21408 |
IN |
denotes |
of |
T14998 |
21409-21416 |
NN |
denotes |
Annexin |
T14999 |
21417-21419 |
NN |
denotes |
A7 |
T15000 |
21419-21420 |
. |
denotes |
. |
T15001 |
21420-21545 |
sentence |
denotes |
(A) Extraction of Annexin A7 from nuclei of neuronal (PC-12, N2A) and an astroglial (C6) cell line using a hypotonic buffer. |
T15002 |
21421-21422 |
-LRB- |
denotes |
( |
T15003 |
21422-21423 |
LS |
denotes |
A |
T15004 |
21425-21435 |
NN |
denotes |
Extraction |
T15005 |
21423-21424 |
-RRB- |
denotes |
) |
T15006 |
21436-21438 |
IN |
denotes |
of |
T15007 |
21439-21446 |
NN |
denotes |
Annexin |
T15008 |
21447-21449 |
NN |
denotes |
A7 |
T15009 |
21450-21454 |
IN |
denotes |
from |
T15010 |
21455-21461 |
NNS |
denotes |
nuclei |
T15011 |
21462-21464 |
IN |
denotes |
of |
T15012 |
21465-21473 |
JJ |
denotes |
neuronal |
T15013 |
21474-21475 |
-LRB- |
denotes |
( |
T15014 |
21475-21477 |
NN |
denotes |
PC |
T15015 |
21477-21478 |
HYPH |
denotes |
- |
T15016 |
21478-21480 |
CD |
denotes |
12 |
T15017 |
21480-21482 |
, |
denotes |
, |
T15018 |
21482-21485 |
NN |
denotes |
N2A |
T15019 |
21485-21486 |
-RRB- |
denotes |
) |
T15020 |
21487-21490 |
CC |
denotes |
and |
T15021 |
21491-21493 |
DT |
denotes |
an |
T15022 |
21494-21504 |
JJ |
denotes |
astroglial |
T15023 |
21515-21519 |
NN |
denotes |
line |
T15024 |
21505-21506 |
-LRB- |
denotes |
( |
T15025 |
21506-21508 |
NN |
denotes |
C6 |
T15026 |
21508-21509 |
-RRB- |
denotes |
) |
T15027 |
21510-21514 |
NN |
denotes |
cell |
T15028 |
21520-21525 |
VBG |
denotes |
using |
T15029 |
21526-21527 |
DT |
denotes |
a |
T15030 |
21538-21544 |
NN |
denotes |
buffer |
T15031 |
21528-21537 |
JJ |
denotes |
hypotonic |
T15032 |
21544-21545 |
. |
denotes |
. |
T15033 |
21545-21693 |
sentence |
denotes |
Samples of total cells (c), and corresponding amounts of nuclear membranes (m) and nucleoplasm (p) were subjected to SDS-PAGE and Western blotting. |
T15034 |
21546-21553 |
NNS |
denotes |
Samples |
T15035 |
21650-21659 |
VBN |
denotes |
subjected |
T15036 |
21554-21556 |
IN |
denotes |
of |
T15037 |
21557-21562 |
JJ |
denotes |
total |
T15038 |
21563-21568 |
NNS |
denotes |
cells |
T15039 |
21569-21570 |
-LRB- |
denotes |
( |
T15040 |
21570-21571 |
NN |
denotes |
c |
T15041 |
21571-21572 |
-RRB- |
denotes |
) |
T15042 |
21572-21574 |
, |
denotes |
, |
T15043 |
21574-21577 |
CC |
denotes |
and |
T15044 |
21578-21591 |
JJ |
denotes |
corresponding |
T15045 |
21592-21599 |
NNS |
denotes |
amounts |
T15046 |
21600-21602 |
IN |
denotes |
of |
T15047 |
21603-21610 |
JJ |
denotes |
nuclear |
T15048 |
21611-21620 |
NNS |
denotes |
membranes |
T15049 |
21621-21622 |
-LRB- |
denotes |
( |
T15050 |
21622-21623 |
NN |
denotes |
m |
T15051 |
21623-21624 |
-RRB- |
denotes |
) |
T15052 |
21625-21628 |
CC |
denotes |
and |
T15053 |
21629-21640 |
NN |
denotes |
nucleoplasm |
T15054 |
21641-21642 |
-LRB- |
denotes |
( |
T15055 |
21642-21643 |
NN |
denotes |
p |
T15056 |
21643-21644 |
-RRB- |
denotes |
) |
T15057 |
21645-21649 |
VBD |
denotes |
were |
T15058 |
21660-21662 |
IN |
denotes |
to |
T15059 |
21663-21666 |
NN |
denotes |
SDS |
T15060 |
21667-21671 |
NN |
denotes |
PAGE |
T15061 |
21666-21667 |
HYPH |
denotes |
- |
T15062 |
21672-21675 |
CC |
denotes |
and |
T15063 |
21676-21683 |
NNP |
denotes |
Western |
T15064 |
21684-21692 |
NN |
denotes |
blotting |
T15065 |
21692-21693 |
. |
denotes |
. |
T15066 |
21693-21817 |
sentence |
denotes |
Annexin A7 (AnxA7) isoforms are extractable from the nucleus of the indicated cells (47 kDa and 51 kDa isoform, arrowhead). |
T15067 |
21694-21701 |
NN |
denotes |
Annexin |
T15068 |
21702-21704 |
NN |
denotes |
A7 |
T15069 |
21713-21721 |
NNS |
denotes |
isoforms |
T15070 |
21705-21706 |
-LRB- |
denotes |
( |
T15071 |
21706-21711 |
NN |
denotes |
AnxA7 |
T15072 |
21711-21712 |
-RRB- |
denotes |
) |
T15073 |
21722-21725 |
VBP |
denotes |
are |
T15074 |
21726-21737 |
JJ |
denotes |
extractable |
T15075 |
21738-21742 |
IN |
denotes |
from |
T15076 |
21743-21746 |
DT |
denotes |
the |
T15077 |
21747-21754 |
NN |
denotes |
nucleus |
T15078 |
21755-21757 |
IN |
denotes |
of |
T15079 |
21758-21761 |
DT |
denotes |
the |
T15080 |
21772-21777 |
NNS |
denotes |
cells |
T15081 |
21762-21771 |
VBN |
denotes |
indicated |
T15082 |
21778-21779 |
-LRB- |
denotes |
( |
T15083 |
21806-21815 |
NN |
denotes |
arrowhead |
T15084 |
21779-21781 |
CD |
denotes |
47 |
T15085 |
21782-21785 |
NN |
denotes |
kDa |
T15086 |
21797-21804 |
NN |
denotes |
isoform |
T15087 |
21786-21789 |
CC |
denotes |
and |
T15088 |
21790-21792 |
CD |
denotes |
51 |
T15089 |
21793-21796 |
NN |
denotes |
kDa |
T15090 |
21804-21806 |
, |
denotes |
, |
T15091 |
21815-21816 |
-RRB- |
denotes |
) |
T15092 |
21816-21817 |
. |
denotes |
. |
T15093 |
21817-22049 |
sentence |
denotes |
For control, immunoblotting of LAP2α (75 kDa; non-membrane-bound isoform), Emerin (EM, 34 kDa), and tubulin (TB, 55 kDa) which are specific for nucleoplasm (LAP2α), nuclear membranes (Emerin), and the cytoplasm (tubulin) are shown. |
T15094 |
21818-21821 |
IN |
denotes |
For |
T15095 |
22043-22048 |
VBN |
denotes |
shown |
T15096 |
21822-21829 |
NN |
denotes |
control |
T15097 |
21829-21831 |
, |
denotes |
, |
T15098 |
21831-21845 |
NN |
denotes |
immunoblotting |
T15099 |
21846-21848 |
IN |
denotes |
of |
T15100 |
21849-21854 |
NN |
denotes |
LAP2α |
T15101 |
21855-21856 |
-LRB- |
denotes |
( |
T15102 |
21883-21890 |
NN |
denotes |
isoform |
T15103 |
21856-21858 |
CD |
denotes |
75 |
T15104 |
21859-21862 |
NN |
denotes |
kDa |
T15105 |
21862-21863 |
: |
denotes |
; |
T15106 |
21864-21876 |
JJ |
denotes |
non-membrane |
T15107 |
21877-21882 |
VBN |
denotes |
bound |
T15108 |
21876-21877 |
HYPH |
denotes |
- |
T15109 |
21890-21891 |
-RRB- |
denotes |
) |
T15110 |
21891-21893 |
, |
denotes |
, |
T15111 |
21893-21899 |
NN |
denotes |
Emerin |
T15112 |
21900-21901 |
-LRB- |
denotes |
( |
T15113 |
21901-21903 |
NN |
denotes |
EM |
T15114 |
21903-21905 |
, |
denotes |
, |
T15115 |
21908-21911 |
NN |
denotes |
kDa |
T15116 |
21905-21907 |
CD |
denotes |
34 |
T15117 |
21911-21912 |
-RRB- |
denotes |
) |
T15118 |
21912-21914 |
, |
denotes |
, |
T15119 |
21914-21917 |
CC |
denotes |
and |
T15120 |
21918-21925 |
NN |
denotes |
tubulin |
T15121 |
21926-21927 |
-LRB- |
denotes |
( |
T15122 |
21927-21929 |
NN |
denotes |
TB |
T15123 |
21929-21931 |
, |
denotes |
, |
T15124 |
21934-21937 |
NN |
denotes |
kDa |
T15125 |
21931-21933 |
CD |
denotes |
55 |
T15126 |
21937-21938 |
-RRB- |
denotes |
) |
T15127 |
21939-21944 |
WDT |
denotes |
which |
T15128 |
21945-21948 |
VBP |
denotes |
are |
T15129 |
21949-21957 |
JJ |
denotes |
specific |
T15130 |
21958-21961 |
IN |
denotes |
for |
T15131 |
21962-21973 |
NN |
denotes |
nucleoplasm |
T15132 |
21974-21975 |
-LRB- |
denotes |
( |
T15133 |
21975-21980 |
NN |
denotes |
LAP2α |
T15134 |
21980-21981 |
-RRB- |
denotes |
) |
T15135 |
21981-21983 |
, |
denotes |
, |
T15136 |
21983-21990 |
JJ |
denotes |
nuclear |
T15137 |
21991-22000 |
NNS |
denotes |
membranes |
T15138 |
22001-22002 |
-LRB- |
denotes |
( |
T15139 |
22002-22008 |
NN |
denotes |
Emerin |
T15140 |
22008-22009 |
-RRB- |
denotes |
) |
T15141 |
22009-22011 |
, |
denotes |
, |
T15142 |
22011-22014 |
CC |
denotes |
and |
T15143 |
22015-22018 |
DT |
denotes |
the |
T15144 |
22019-22028 |
NN |
denotes |
cytoplasm |
T15145 |
22029-22030 |
-LRB- |
denotes |
( |
T15146 |
22030-22037 |
NN |
denotes |
tubulin |
T15147 |
22037-22038 |
-RRB- |
denotes |
) |
T15148 |
22039-22042 |
VBP |
denotes |
are |
T15149 |
22048-22049 |
. |
denotes |
. |
T15150 |
22049-22106 |
sentence |
denotes |
Note that the nucleoplasm is only partially extractable. |
T15151 |
22050-22054 |
VB |
denotes |
Note |
T15152 |
22055-22059 |
IN |
denotes |
that |
T15153 |
22076-22078 |
VBZ |
denotes |
is |
T15154 |
22060-22063 |
DT |
denotes |
the |
T15155 |
22064-22075 |
NN |
denotes |
nucleoplasm |
T15156 |
22079-22083 |
RB |
denotes |
only |
T15157 |
22084-22093 |
RB |
denotes |
partially |
T15158 |
22094-22105 |
JJ |
denotes |
extractable |
T15159 |
22105-22106 |
. |
denotes |
. |
T15160 |
22106-22161 |
sentence |
denotes |
(B) Immunofluorescence images of the PC-12 cells used. |
T15161 |
22107-22108 |
-LRB- |
denotes |
( |
T15162 |
22108-22109 |
LS |
denotes |
B |
T15163 |
22130-22136 |
NNS |
denotes |
images |
T15164 |
22109-22110 |
-RRB- |
denotes |
) |
T15165 |
22111-22129 |
NN |
denotes |
Immunofluorescence |
T15166 |
22137-22139 |
IN |
denotes |
of |
T15167 |
22140-22143 |
DT |
denotes |
the |
T15168 |
22150-22155 |
NNS |
denotes |
cells |
T15169 |
22144-22146 |
NN |
denotes |
PC |
T15170 |
22146-22147 |
HYPH |
denotes |
- |
T15171 |
22147-22149 |
CD |
denotes |
12 |
T15172 |
22156-22160 |
VBN |
denotes |
used |
T15173 |
22160-22161 |
. |
denotes |
. |
T15174 |
22161-22347 |
sentence |
denotes |
Cells were fixed with paraformaldehyde, permeabilized with Triton X-100, pretreated with trypsin as indicated, and stained with Annexin A7 specific mAb 203–217, DAPI in blue, bar 10 μm. |
T15175 |
22162-22167 |
NNS |
denotes |
Cells |
T15176 |
22173-22178 |
VBN |
denotes |
fixed |
T15177 |
22168-22172 |
VBD |
denotes |
were |
T15178 |
22179-22183 |
IN |
denotes |
with |
T15179 |
22184-22200 |
NN |
denotes |
paraformaldehyde |
T15180 |
22200-22202 |
, |
denotes |
, |
T15181 |
22202-22215 |
VBN |
denotes |
permeabilized |
T15182 |
22216-22220 |
IN |
denotes |
with |
T15183 |
22221-22227 |
NN |
denotes |
Triton |
T15184 |
22228-22229 |
NN |
denotes |
X |
T15185 |
22229-22230 |
HYPH |
denotes |
- |
T15186 |
22230-22233 |
CD |
denotes |
100 |
T15187 |
22233-22235 |
, |
denotes |
, |
T15188 |
22235-22245 |
VBN |
denotes |
pretreated |
T15189 |
22246-22250 |
IN |
denotes |
with |
T15190 |
22251-22258 |
NN |
denotes |
trypsin |
T15191 |
22259-22261 |
IN |
denotes |
as |
T15192 |
22262-22271 |
VBN |
denotes |
indicated |
T15193 |
22271-22273 |
, |
denotes |
, |
T15194 |
22273-22276 |
CC |
denotes |
and |
T15195 |
22277-22284 |
VBN |
denotes |
stained |
T15196 |
22285-22289 |
IN |
denotes |
with |
T15197 |
22290-22297 |
NN |
denotes |
Annexin |
T15198 |
22298-22300 |
NN |
denotes |
A7 |
T15199 |
22301-22309 |
JJ |
denotes |
specific |
T15200 |
22314-22317 |
CD |
denotes |
203 |
T15201 |
22310-22313 |
NN |
denotes |
mAb |
T15202 |
22317-22318 |
SYM |
denotes |
– |
T15203 |
22318-22321 |
CD |
denotes |
217 |
T15204 |
22321-22323 |
, |
denotes |
, |
T15205 |
22344-22346 |
NN |
denotes |
μm |
T15206 |
22323-22327 |
NN |
denotes |
DAPI |
T15207 |
22328-22330 |
IN |
denotes |
in |
T15208 |
22331-22335 |
JJ |
denotes |
blue |
T15209 |
22335-22337 |
, |
denotes |
, |
T15210 |
22337-22340 |
NN |
denotes |
bar |
T15211 |
22341-22343 |
CD |
denotes |
10 |
T15212 |
22346-22347 |
. |
denotes |
. |
T2415 |
5113-5121 |
VBD |
denotes |
verified |
T2416 |
5092-5096 |
IN |
denotes |
with |
T2417 |
5097-5098 |
DT |
denotes |
a |
T2418 |
5107-5112 |
NN |
denotes |
probe |
T2419 |
5099-5100 |
NN |
denotes |
β |
T2420 |
5101-5106 |
NN |
denotes |
actin |
T2421 |
5100-5101 |
HYPH |
denotes |
- |
T2422 |
5221-5223 |
VBZ |
denotes |
is |
T2423 |
5122-5127 |
JJ |
denotes |
equal |
T2424 |
5128-5135 |
NN |
denotes |
loading |
T2425 |
5135-5136 |
: |
denotes |
; |
T2526 |
6495-6500 |
NN |
denotes |
stage |
T2527 |
6486-6494 |
NN |
denotes |
cylinder |
T2528 |
6501-6503 |
IN |
denotes |
at |
T2529 |
6504-6506 |
NN |
denotes |
E5 |
T2530 |
6507-6511 |
IN |
denotes |
with |
T2531 |
6512-6513 |
DT |
denotes |
a |
T2532 |
6532-6542 |
NN |
denotes |
expression |
T2533 |
6514-6520 |
JJR |
denotes |
weaker |
T2534 |
6521-6528 |
NN |
denotes |
Annexin |
T3828 |
14041-14052 |
NN |
denotes |
pyramidalis |
T3829 |
14033-14040 |
NN |
denotes |
stratum |
T3830 |
14053-14056 |
CC |
denotes |
and |
T3831 |
14057-14059 |
IN |
denotes |
in |
T3832 |
14060-14063 |
DT |
denotes |
the |
T3833 |
14072-14077 |
NN |
denotes |
gyrus |
T3834 |
14064-14071 |
NN |
denotes |
dentate |
T3835 |
14078-14081 |
CC |
denotes |
and |
T3836 |
14082-14086 |
RB |
denotes |
also |
T3837 |
14087-14088 |
DT |
denotes |
a |
T3838 |
14105-14113 |
NN |
denotes |
staining |
T3839 |
14089-14093 |
JJ |
denotes |
weak |
T3840 |
14094-14104 |
JJ |
denotes |
astrocytic |
T3841 |
14114-14115 |
-LRB- |
denotes |
( |
T3842 |
14120-14122 |
NN |
denotes |
4C |
T2403 |
5045-5051 |
NNS |
denotes |
levels |
T2404 |
5029-5036 |
NN |
denotes |
Annexin |
T2405 |
5037-5039 |
NN |
denotes |
A7 |
T2406 |
5040-5044 |
NN |
denotes |
mRNA |
T2407 |
5052-5054 |
IN |
denotes |
in |
T2408 |
5055-5058 |
DT |
denotes |
the |
T2409 |
5074-5080 |
NNS |
denotes |
stages |
T2410 |
5059-5063 |
CD |
denotes |
four |
T2411 |
5064-5073 |
JJ |
denotes |
embryonic |
T2412 |
5080-5081 |
. |
denotes |
. |
T2413 |
5081-5256 |
sentence |
denotes |
Reprobing with a β-actin probe verified equal loading; the appearance of a faster migrating mRNA species in addition to the 2.0 kb species is characteristic for β-actin [12]. |
T2414 |
5082-5091 |
VBG |
denotes |
Reprobing |
T2485 |
5410-6334 |
sentence |
denotes |
Figure 1 Expression of Annexin A7. (A) Northern blot analysis of RNA from ES-cells and embryos E7, E11, E15, E17 probed with a full length annexin A7 cDNA. Transcripts of 1.8 and 2.4 kb are detected in all stages examined (upper panel). Reprobing with a β-actin probe verified equal loading (lower panel, 2.0 kb band). (B) Murine ES cells (ES) express only the small Annexin A7 isoform. Both isoforms are expressed in neuroblastoma neuro-2a cells (N2A), heart (HRT) and brain tissue (CNS) from adult mice. Proteins from neuro-2a and ES cells, heart and brain were resolved by SDS polyacrylamide gel electrophoresis (12% acrylamide) and transferred to nitrocellulose membranes. The Western blot was probed with mAb 203–217 followed by incubation with a peroxidase coupled secondary antibody. Detection was with enhanced chemiluminescence. Immunohistochemistry confirmed the presence of the protein during early development. |
T2486 |
6250-6270 |
NN |
denotes |
Immunohistochemistry |
T2487 |
6271-6280 |
VBD |
denotes |
confirmed |
T2488 |
6281-6284 |
DT |
denotes |
the |
T2489 |
6285-6293 |
NN |
denotes |
presence |
T2490 |
6294-6296 |
IN |
denotes |
of |
T2491 |
6297-6300 |
DT |
denotes |
the |
T2492 |
6301-6308 |
NN |
denotes |
protein |
T2493 |
6309-6315 |
IN |
denotes |
during |
T2494 |
6316-6321 |
JJ |
denotes |
early |
T2495 |
6322-6333 |
NN |
denotes |
development |
T2496 |
6333-6334 |
. |
denotes |
. |
T2497 |
6334-6571 |
sentence |
denotes |
Annexin A7 immunoreactivity using mAb 203–217, which specifically recognizes both isoforms of Annexin A7, can be observed in the two cell types of the cylinder stage at E5 with a weaker Annexin A7 expression in the ectoderm (Fig. 2A–C). |
T2498 |
6335-6342 |
NN |
denotes |
Annexin |
T2499 |
6343-6345 |
NN |
denotes |
A7 |
T2500 |
6346-6362 |
NN |
denotes |
immunoreactivity |
T2501 |
6448-6456 |
VBN |
denotes |
observed |
T2502 |
6363-6368 |
VBG |
denotes |
using |
T2503 |
6369-6372 |
NN |
denotes |
mAb |
T2504 |
6373-6376 |
CD |
denotes |
203 |
T2505 |
6376-6377 |
SYM |
denotes |
– |
T2506 |
6377-6380 |
CD |
denotes |
217 |
T2507 |
6380-6382 |
, |
denotes |
, |
T2508 |
6382-6387 |
WDT |
denotes |
which |
T2509 |
6401-6411 |
VBZ |
denotes |
recognizes |
T2510 |
6388-6400 |
RB |
denotes |
specifically |
T2511 |
6412-6416 |
DT |
denotes |
both |
T2512 |
6417-6425 |
NNS |
denotes |
isoforms |
T2513 |
6426-6428 |
IN |
denotes |
of |
T2514 |
6429-6436 |
NN |
denotes |
Annexin |
T2515 |
6437-6439 |
NN |
denotes |
A7 |
T2516 |
6439-6441 |
, |
denotes |
, |
T2517 |
6441-6444 |
MD |
denotes |
can |
T2518 |
6445-6447 |
VB |
denotes |
be |
T2519 |
6457-6459 |
IN |
denotes |
in |
T2520 |
6460-6463 |
DT |
denotes |
the |
T2521 |
6473-6478 |
NNS |
denotes |
types |
T2522 |
6464-6467 |
CD |
denotes |
two |
T2523 |
6468-6472 |
NN |
denotes |
cell |
T2524 |
6479-6481 |
IN |
denotes |
of |
T2525 |
6482-6485 |
DT |
denotes |
the |
T260 |
0-7 |
JJ |
denotes |
Nuclear |
T3743 |
11747-11758 |
JJ |
denotes |
neocortical |
T3744 |
11759-11768 |
JJ |
denotes |
molecular |
T3745 |
11775-11776 |
-LRB- |
denotes |
( |
T3746 |
11776-11781 |
NN |
denotes |
layer |
T3747 |
11782-11783 |
CD |
denotes |
I |
T3748 |
11783-11784 |
-RRB- |
denotes |
) |
T3749 |
11785-11790 |
WDT |
denotes |
which |
T3750 |
11804-11812 |
VBZ |
denotes |
exhibits |
T3751 |
11791-11803 |
RB |
denotes |
additionally |
T3752 |
11813-11815 |
DT |
denotes |
an |
T3753 |
11824-11830 |
NN |
denotes |
signal |
T3754 |
11816-11823 |
JJ |
denotes |
intense |
T3755 |
11831-11833 |
IN |
denotes |
in |
T3756 |
11834-11837 |
DT |
denotes |
the |
T3757 |
11838-11847 |
NN |
denotes |
cytoplasm |
T3758 |
11848-11851 |
CC |
denotes |
and |
T3759 |
11852-11854 |
IN |
denotes |
in |
T3760 |
11855-11863 |
JJ |
denotes |
cellular |
T3761 |
11864-11872 |
NNS |
denotes |
branches |
T3762 |
11872-11873 |
. |
denotes |
. |
T3763 |
11873-12070 |
sentence |
denotes |
A further cell type of the isocortex showed a strong immunoreactivity in both, nucleus and cytosol, and was identified as pyramidal neuron based on morphology and absence of GFAP immunoreactivity. |
T3764 |
11874-11875 |
DT |
denotes |
A |
T3765 |
11889-11893 |
NN |
denotes |
type |
T3766 |
11876-11883 |
JJ |
denotes |
further |
T3767 |
11884-11888 |
NN |
denotes |
cell |
T3768 |
11911-11917 |
VBD |
denotes |
showed |
T3769 |
11894-11896 |
IN |
denotes |
of |
T3770 |
11897-11900 |
DT |
denotes |
the |
T3771 |
11901-11910 |
NN |
denotes |
isocortex |
T3772 |
11918-11919 |
DT |
denotes |
a |
T3773 |
11927-11943 |
NN |
denotes |
immunoreactivity |
T3774 |
11920-11926 |
JJ |
denotes |
strong |
T3815 |
13949-14124 |
sentence |
denotes |
In the hippocampal formation we detected prominent Annexin A7 immunostaining in the stratum pyramidalis and in the dentate gyrus and also a weak astrocytic staining (Fig. 4C). |
T3816 |
13949-13951 |
IN |
denotes |
In |
T3817 |
13981-13989 |
VBD |
denotes |
detected |
T3818 |
13952-13955 |
DT |
denotes |
the |
T3819 |
13968-13977 |
NN |
denotes |
formation |
T3820 |
13956-13967 |
JJ |
denotes |
hippocampal |
T3821 |
13978-13980 |
PRP |
denotes |
we |
T3822 |
13990-13999 |
JJ |
denotes |
prominent |
T3823 |
14011-14025 |
NN |
denotes |
immunostaining |
T3824 |
14000-14007 |
NN |
denotes |
Annexin |
T3825 |
14008-14010 |
NN |
denotes |
A7 |
T3826 |
14026-14028 |
IN |
denotes |
in |
T3827 |
14029-14032 |
DT |
denotes |
the |
T6504 |
22861-22871 |
JJ |
denotes |
Endodermal |
T7882 |
30398-30400 |
IN |
denotes |
by |
T7883 |
30401-30404 |
PRP$ |
denotes |
its |
T7884 |
30405-30417 |
NN |
denotes |
purification |
T7885 |
30418-30420 |
IN |
denotes |
as |
T7886 |
30421-30425 |
NN |
denotes |
part |
T7887 |
30426-30428 |
IN |
denotes |
of |
R39 |
T298 |
T297 |
prep |
of,presence |
R9 |
T263 |
T261 |
prep |
of,localization |
R10 |
T264 |
T265 |
compound |
Annexin,A7 |
R11 |
T265 |
T263 |
pobj |
A7,of |
R12 |
T266 |
T261 |
prep |
during,localization |
R13 |
T267 |
T268 |
amod |
murine,brain |
R14 |
T268 |
T269 |
compound |
brain,development |
R15 |
T269 |
T266 |
pobj |
development,during |
R16 |
T274 |
T275 |
compound |
Annexin,A7 |
R17 |
T275 |
T276 |
nsubj |
A7,is |
R18 |
T277 |
T278 |
det |
a,member |
R19 |
T278 |
T276 |
attr |
member,is |
R20 |
T279 |
T278 |
prep |
of,member |
R21 |
T280 |
T281 |
det |
the,family |
R22 |
T281 |
T279 |
pobj |
family,of |
R23 |
T282 |
T283 |
compound |
annexin,protein |
R24 |
T283 |
T281 |
compound |
protein,family |
R25 |
T284 |
T281 |
punct |
", ",family |
R26 |
T285 |
T286 |
dep |
which,characterized |
R27 |
T286 |
T281 |
advcl |
characterized,family |
R28 |
T287 |
T286 |
auxpass |
is,characterized |
R29 |
T288 |
T286 |
agent |
by,characterized |
R30 |
T289 |
T290 |
poss |
its,ability |
R31 |
T290 |
T288 |
pobj |
ability,by |
R32 |
T291 |
T292 |
aux |
to,interact |
R33 |
T292 |
T290 |
acl |
interact,ability |
R34 |
T293 |
T292 |
prep |
with,interact |
R35 |
T294 |
T293 |
pobj |
phospholipids,with |
R36 |
T295 |
T294 |
prep |
in,phospholipids |
R37 |
T296 |
T297 |
det |
the,presence |
R38 |
T297 |
T295 |
pobj |
presence,in |
R40 |
T299 |
T300 |
compound |
Ca2+,ions |
R41 |
T300 |
T298 |
pobj |
ions,of |
R42 |
T301 |
T300 |
punct |
-,ions |
R43 |
T302 |
T286 |
cc |
and,characterized |
R44 |
T303 |
T304 |
dep |
which,thought |
R45 |
T304 |
T286 |
conj |
thought,characterized |
R46 |
T305 |
T304 |
auxpass |
is,thought |
R47 |
T306 |
T307 |
aux |
to,function |
R48 |
T307 |
T304 |
xcomp |
function,thought |
R49 |
T308 |
T307 |
prep |
in,function |
R50 |
T309 |
T310 |
compound |
Ca2+,homeostasis |
R51 |
T310 |
T308 |
pobj |
homeostasis,in |
R52 |
T311 |
T310 |
punct |
-,homeostasis |
R53 |
T312 |
T276 |
punct |
.,is |
R54 |
T314 |
T315 |
nsubj |
Results,showed |
R55 |
T316 |
T314 |
prep |
from,Results |
R56 |
T317 |
T318 |
compound |
mutant,mice |
R57 |
T318 |
T316 |
pobj |
mice,from |
R58 |
T319 |
T320 |
amod |
altered,propagation |
R59 |
T320 |
T315 |
dobj |
propagation,showed |
R60 |
T321 |
T322 |
compound |
Ca2+,wave |
R61 |
T322 |
T320 |
compound |
wave,propagation |
R62 |
T323 |
T322 |
punct |
-,wave |
R63 |
T324 |
T315 |
prep |
in,showed |
R64 |
T325 |
T324 |
pobj |
astrocytes,in |
R65 |
T326 |
T315 |
punct |
.,showed |
R66 |
T328 |
T329 |
mark |
As,investigated |
R67 |
T329 |
T343 |
advcl |
investigated,focused |
R68 |
T330 |
T331 |
det |
the,appearance |
R69 |
T331 |
T329 |
nsubjpass |
appearance,investigated |
R70 |
T332 |
T331 |
cc |
and,appearance |
R71 |
T333 |
T331 |
conj |
distribution,appearance |
R72 |
T334 |
T331 |
prep |
of,appearance |
R73 |
T335 |
T336 |
compound |
Annexin,A7 |
R74 |
T336 |
T334 |
pobj |
A7,of |
R75 |
T337 |
T331 |
prep |
during,appearance |
R76 |
T338 |
T339 |
compound |
brain,development |
R77 |
T339 |
T337 |
pobj |
development,during |
R78 |
T340 |
T329 |
aux |
has,investigated |
R79 |
T341 |
T329 |
neg |
not,investigated |
R80 |
T342 |
T329 |
auxpass |
been,investigated |
R81 |
T344 |
T345 |
advmod |
so,far |
R82 |
T345 |
T329 |
advmod |
far,investigated |
R83 |
T346 |
T343 |
punct |
", ",focused |
R84 |
T347 |
T343 |
nsubj |
we,focused |
R85 |
T348 |
T343 |
prep |
on,focused |
R86 |
T349 |
T350 |
det |
the,distribution |
R87 |
T350 |
T348 |
pobj |
distribution,on |
R88 |
T351 |
T350 |
prep |
of,distribution |
R89 |
T352 |
T353 |
compound |
Annexin,A7 |
R90 |
T353 |
T354 |
compound |
A7,protein |
R91 |
T354 |
T351 |
pobj |
protein,of |
R92 |
T355 |
T350 |
prep |
during,distribution |
R93 |
T356 |
T357 |
compound |
mouse,embryogenesis |
R94 |
T357 |
T355 |
pobj |
embryogenesis,during |
R95 |
T358 |
T343 |
prep |
in,focused |
R96 |
T359 |
T360 |
det |
the,system |
R97 |
T360 |
T358 |
pobj |
system,in |
R98 |
T361 |
T360 |
amod |
developing,system |
R99 |
T362 |
T360 |
amod |
central,system |
R100 |
T363 |
T360 |
amod |
nervous,system |
R101 |
T364 |
T358 |
cc |
and,in |
R102 |
T365 |
T358 |
conj |
in,in |
R103 |
T366 |
T367 |
det |
the,brain |
R104 |
T367 |
T365 |
pobj |
brain,in |
R105 |
T368 |
T367 |
amod |
adult,brain |
R106 |
T369 |
T367 |
compound |
mouse,brain |
R107 |
T370 |
T343 |
punct |
.,focused |
R108 |
T374 |
T375 |
compound |
Annexin,A7 |
R109 |
T375 |
T376 |
nsubjpass |
A7,expressed |
R110 |
T377 |
T376 |
auxpass |
is,expressed |
R111 |
T378 |
T376 |
prep |
in,expressed |
R112 |
T379 |
T378 |
pobj |
cells,in |
R113 |
T380 |
T379 |
prep |
of,cells |
R114 |
T381 |
T382 |
det |
the,brain |
R115 |
T382 |
T380 |
pobj |
brain,of |
R116 |
T383 |
T382 |
amod |
developing,brain |
R117 |
T384 |
T385 |
advmod |
where,observed |
R118 |
T385 |
T382 |
relcl |
observed,brain |
R119 |
T386 |
T387 |
det |
a,change |
R120 |
T387 |
T385 |
nsubjpass |
change,observed |
R121 |
T388 |
T387 |
prep |
in,change |
R122 |
T389 |
T390 |
poss |
its,localization |
R123 |
T390 |
T388 |
pobj |
localization,in |
R124 |
T391 |
T390 |
amod |
subcellular,localization |
R125 |
T392 |
T387 |
prep |
from,change |
R126 |
T393 |
T392 |
pobj |
cytoplasm,from |
R127 |
T394 |
T392 |
prep |
to,from |
R128 |
T395 |
T394 |
pobj |
nucleus,to |
R129 |
T396 |
T385 |
auxpass |
was,observed |
R130 |
T397 |
T376 |
punct |
.,expressed |
R131 |
T399 |
T400 |
prep |
In,depends |
R132 |
T401 |
T402 |
det |
the,CNS |
R133 |
T402 |
T399 |
pobj |
CNS,In |
R134 |
T403 |
T402 |
amod |
adult,CNS |
R135 |
T404 |
T400 |
punct |
", ",depends |
R136 |
T405 |
T406 |
det |
the,distribution |
R137 |
T406 |
T400 |
nsubj |
distribution,depends |
R138 |
T407 |
T406 |
amod |
subcellular,distribution |
R139 |
T408 |
T406 |
prep |
of,distribution |
R140 |
T409 |
T410 |
compound |
Annexin,A7 |
R141 |
T410 |
T408 |
pobj |
A7,of |
R142 |
T411 |
T400 |
prep |
on,depends |
R143 |
T412 |
T413 |
det |
the,type |
R144 |
T413 |
T411 |
pobj |
type,on |
R145 |
T414 |
T413 |
compound |
cell,type |
R146 |
T415 |
T400 |
punct |
.,depends |
R147 |
T417 |
T418 |
prep |
By,detected |
R148 |
T419 |
T420 |
compound |
immunohistochemistry,analysis |
R149 |
T420 |
T417 |
pobj |
analysis,By |
R150 |
T421 |
T422 |
compound |
Annexin,A7 |
R151 |
T422 |
T418 |
nsubjpass |
A7,detected |
R152 |
T423 |
T418 |
auxpass |
was,detected |
R153 |
T424 |
T418 |
prep |
in,detected |
R154 |
T425 |
T426 |
det |
the,cytosol |
R155 |
T426 |
T424 |
pobj |
cytosol,in |
R156 |
T427 |
T426 |
prep |
of,cytosol |
R157 |
T428 |
T429 |
amod |
undifferentiated,cells |
R158 |
T429 |
T427 |
pobj |
cells,of |
R159 |
T430 |
T418 |
prep |
at,detected |
R160 |
T431 |
T432 |
amod |
embryonic,E5 |
R161 |
T432 |
T430 |
pobj |
E5,at |
R162 |
T433 |
T432 |
compound |
days,E5 |
R163 |
T434 |
T435 |
punct |
–,E8 |
R164 |
T435 |
T432 |
prep |
E8,E5 |
R165 |
T436 |
T418 |
punct |
.,detected |
R166 |
T438 |
T439 |
prep |
At,is |
R167 |
T440 |
T438 |
pobj |
E11,At |
R168 |
T441 |
T442 |
punct |
–,E15 |
R169 |
T442 |
T440 |
prep |
E15,E11 |
R170 |
T443 |
T444 |
det |
the,protein |
R171 |
T444 |
T439 |
nsubj |
protein,is |
R172 |
T445 |
T439 |
advmod |
still,is |
R173 |
T446 |
T439 |
acomp |
present,is |
R174 |
T447 |
T439 |
prep |
in,is |
R175 |
T448 |
T449 |
det |
the,cytosol |
R176 |
T449 |
T447 |
pobj |
cytosol,in |
R177 |
T450 |
T449 |
prep |
of,cytosol |
R178 |
T451 |
T450 |
pobj |
cells,of |
R179 |
T452 |
T453 |
advmod |
predominantly,located |
R180 |
T453 |
T451 |
acl |
located,cells |
R181 |
T454 |
T453 |
prep |
in,located |
R182 |
T455 |
T456 |
det |
the,zone |
R183 |
T456 |
T454 |
pobj |
zone,in |
R184 |
T457 |
T456 |
amod |
ventricular,zone |
R185 |
T458 |
T456 |
amod |
germinative,zone |
R186 |
T459 |
T456 |
acl |
surrounding,zone |
R187 |
T460 |
T461 |
det |
the,ventricle |
R188 |
T461 |
T459 |
dobj |
ventricle,surrounding |
R189 |
T462 |
T461 |
amod |
lateral,ventricle |
R190 |
T463 |
T439 |
punct |
.,is |
R191 |
T465 |
T466 |
advmod |
Later,on |
R192 |
T466 |
T467 |
advmod |
on,translocates |
R193 |
T468 |
T467 |
punct |
", ",translocates |
R194 |
T469 |
T467 |
prep |
at,translocates |
R195 |
T470 |
T471 |
amod |
embryonic,E16 |
R196 |
T471 |
T469 |
pobj |
E16,at |
R197 |
T472 |
T471 |
compound |
day,E16 |
R198 |
T473 |
T467 |
punct |
", ",translocates |
R199 |
T474 |
T475 |
compound |
Annexin,A7 |
R200 |
T475 |
T467 |
nsubj |
A7,translocates |
R201 |
T476 |
T475 |
prep |
in,A7 |
R202 |
T477 |
T476 |
pobj |
cells,in |
R203 |
T478 |
T477 |
prep |
of,cells |
R204 |
T479 |
T480 |
det |
the,zone |
R205 |
T480 |
T478 |
pobj |
zone,of |
R206 |
T481 |
T480 |
amod |
intermediate,zone |
R207 |
T482 |
T481 |
cc |
and,intermediate |
R208 |
T483 |
T481 |
conj |
marginal,intermediate |
R209 |
T484 |
T480 |
prep |
of,zone |
R210 |
T485 |
T486 |
det |
the,neopallium |
R211 |
T486 |
T484 |
pobj |
neopallium,of |
R212 |
T487 |
T467 |
prep |
to,translocates |
R213 |
T488 |
T489 |
det |
the,nucleus |
R214 |
T489 |
T487 |
pobj |
nucleus,to |
R215 |
T490 |
T467 |
punct |
.,translocates |
R216 |
T492 |
T493 |
amod |
Neuronal,cells |
R217 |
T493 |
T494 |
nsubj |
cells,present |
R218 |
T495 |
T493 |
prep |
of,cells |
R219 |
T496 |
T497 |
det |
all,areas |
R220 |
T497 |
T495 |
pobj |
areas,of |
R221 |
T498 |
T497 |
prep |
in,areas |
R222 |
T499 |
T500 |
det |
the,brain |
R223 |
T500 |
T498 |
pobj |
brain,in |
R224 |
T501 |
T500 |
amod |
adult,brain |
R225 |
T502 |
T503 |
compound |
Annexin,A7 |
R226 |
T503 |
T494 |
dobj |
A7,present |
R227 |
T504 |
T494 |
prep |
in,present |
R228 |
T505 |
T506 |
det |
the,nucleus |
R229 |
T506 |
T504 |
pobj |
nucleus,in |
R230 |
T507 |
T494 |
punct |
", ",present |
R231 |
T508 |
T509 |
mark |
whereas,exhibit |
R232 |
T509 |
T494 |
advcl |
exhibit,present |
R233 |
T510 |
T511 |
amod |
glial,protein |
R234 |
T511 |
T514 |
npadvmod |
protein,positive |
R235 |
T512 |
T511 |
amod |
fibrillary,protein |
R236 |
T513 |
T511 |
amod |
acidic,protein |
R237 |
T514 |
T519 |
amod |
positive,astrocytes |
R238 |
T515 |
T511 |
punct |
(,protein |
R239 |
T516 |
T511 |
appos |
GFAP,protein |
R240 |
T517 |
T511 |
punct |
),protein |
R241 |
T518 |
T514 |
punct |
-,positive |
R242 |
T519 |
T509 |
nsubj |
astrocytes,exhibit |
R243 |
T520 |
T521 |
preconj |
both,cytoplasmic |
R244 |
T521 |
T509 |
dobj |
cytoplasmic,exhibit |
R245 |
T522 |
T521 |
punct |
", ",cytoplasmic |
R246 |
T523 |
T521 |
det |
a,cytoplasmic |
R247 |
T524 |
T521 |
cc |
and,cytoplasmic |
R248 |
T525 |
T526 |
nmod |
nuclear,staining |
R249 |
T526 |
T521 |
conj |
staining,cytoplasmic |
R250 |
T527 |
T494 |
punct |
.,present |
R251 |
T529 |
T530 |
det |
The,presence |
R252 |
T530 |
T531 |
nsubjpass |
presence,confirmed |
R253 |
T532 |
T530 |
prep |
of,presence |
R254 |
T533 |
T534 |
amod |
nuclear,A7 |
R255 |
T534 |
T532 |
pobj |
A7,of |
R256 |
T535 |
T534 |
compound |
Annexin,A7 |
R257 |
T536 |
T531 |
auxpass |
was,confirmed |
R258 |
T537 |
T531 |
prep |
by,confirmed |
R259 |
T538 |
T537 |
pobj |
extraction,by |
R260 |
T539 |
T538 |
prep |
of,extraction |
R261 |
T540 |
T541 |
det |
the,nucleoplasm |
R262 |
T541 |
T539 |
pobj |
nucleoplasm,of |
R263 |
T542 |
T538 |
prep |
from,extraction |
R264 |
T543 |
T544 |
amod |
isolated,nuclei |
R265 |
T544 |
T542 |
pobj |
nuclei,from |
R266 |
T545 |
T544 |
acl |
obtained,nuclei |
R267 |
T546 |
T545 |
prep |
from,obtained |
R268 |
T547 |
T548 |
amod |
neuronal,lines |
R269 |
T548 |
T546 |
pobj |
lines,from |
R270 |
T549 |
T547 |
cc |
and,neuronal |
R271 |
T550 |
T547 |
conj |
astroglial,neuronal |
R272 |
T551 |
T548 |
compound |
cell,lines |
R273 |
T552 |
T531 |
punct |
.,confirmed |
R274 |
T556 |
T557 |
nsubj |
We,demonstrated |
R275 |
T558 |
T557 |
aux |
have,demonstrated |
R276 |
T559 |
T560 |
det |
a,translocation |
R277 |
T560 |
T557 |
dobj |
translocation,demonstrated |
R278 |
T561 |
T560 |
prep |
of,translocation |
R279 |
T562 |
T563 |
compound |
Annexin,A7 |
R280 |
T563 |
T561 |
pobj |
A7,of |
R281 |
T564 |
T560 |
prep |
to,translocation |
R282 |
T565 |
T564 |
pobj |
nuclei,to |
R283 |
T566 |
T565 |
prep |
of,nuclei |
R284 |
T567 |
T566 |
pobj |
cells,of |
R285 |
T568 |
T560 |
prep |
in,translocation |
R286 |
T569 |
T570 |
amod |
early,development |
R287 |
T570 |
T568 |
pobj |
development,in |
R288 |
T571 |
T572 |
amod |
murine,brain |
R289 |
T572 |
T570 |
compound |
brain,development |
R290 |
T573 |
T560 |
cc |
and,translocation |
R291 |
T574 |
T575 |
det |
the,presence |
R292 |
T575 |
T560 |
conj |
presence,translocation |
R293 |
T576 |
T575 |
prep |
of,presence |
R294 |
T577 |
T578 |
compound |
Annexin,A7 |
R295 |
T578 |
T576 |
pobj |
A7,of |
R296 |
T579 |
T575 |
prep |
in,presence |
R297 |
T580 |
T579 |
pobj |
nuclei,in |
R298 |
T581 |
T580 |
prep |
of,nuclei |
R299 |
T582 |
T583 |
amod |
neuronal,cells |
R300 |
T583 |
T581 |
pobj |
cells,of |
R301 |
T584 |
T575 |
prep |
in,presence |
R302 |
T585 |
T586 |
det |
the,animal |
R303 |
T586 |
T584 |
pobj |
animal,in |
R304 |
T587 |
T586 |
amod |
adult,animal |
R305 |
T588 |
T557 |
punct |
.,demonstrated |
R306 |
T590 |
T591 |
det |
The,role |
R307 |
T591 |
T592 |
nsubj |
role,remains |
R308 |
T593 |
T591 |
prep |
of,role |
R309 |
T594 |
T595 |
compound |
Annexin,A7 |
R310 |
T595 |
T593 |
pobj |
A7,of |
R311 |
T596 |
T591 |
prep |
in,role |
R312 |
T597 |
T596 |
pobj |
nuclei,in |
R313 |
T598 |
T597 |
prep |
of,nuclei |
R314 |
T599 |
T600 |
amod |
differentiating,cells |
R315 |
T600 |
T598 |
pobj |
cells,of |
R316 |
T601 |
T599 |
cc |
and,differentiating |
R317 |
T602 |
T599 |
conj |
mature,differentiating |
R318 |
T603 |
T600 |
amod |
neuronal,cells |
R319 |
T604 |
T592 |
acomp |
elusive,remains |
R320 |
T605 |
T592 |
punct |
.,remains |
R321 |
T260 |
T261 |
amod |
Nuclear,localization |
R324 |
T1249 |
T1250 |
nsubj |
Annexins,form |
R325 |
T1251 |
T1252 |
det |
a,family |
R326 |
T1252 |
T1250 |
dobj |
family,form |
R327 |
T1253 |
T1252 |
prep |
of,family |
R328 |
T1254 |
T1255 |
advmod |
structurally,related |
R329 |
T1255 |
T1256 |
amod |
related,proteins |
R330 |
T1256 |
T1253 |
pobj |
proteins,of |
R331 |
T1257 |
T1256 |
punct |
", ",proteins |
R332 |
T1258 |
T1259 |
dep |
which,bind |
R333 |
T1259 |
T1256 |
relcl |
bind,proteins |
R334 |
T1260 |
T1259 |
prep |
to,bind |
R335 |
T1261 |
T1262 |
advmod |
negatively,charged |
R336 |
T1262 |
T1263 |
amod |
charged,phospholipids |
R337 |
T1263 |
T1260 |
pobj |
phospholipids,to |
R338 |
T1264 |
T1259 |
prep |
in,bind |
R339 |
T1265 |
T1266 |
det |
a,manner |
R340 |
T1266 |
T1264 |
pobj |
manner,in |
R341 |
T1267 |
T1268 |
npadvmod |
Ca2+,dependent |
R342 |
T1268 |
T1266 |
amod |
dependent,manner |
R343 |
T1269 |
T1268 |
punct |
-,dependent |
R344 |
T1270 |
T1271 |
punct |
[,1 |
R345 |
T1271 |
T1250 |
parataxis |
1,form |
R346 |
T1272 |
T1271 |
punct |
],1 |
R347 |
T1273 |
T1250 |
punct |
.,form |
R348 |
T1275 |
T1276 |
nsubjpass |
They,characterized |
R349 |
T1277 |
T1276 |
auxpass |
are,characterized |
R350 |
T1278 |
T1276 |
agent |
by,characterized |
R351 |
T1279 |
T1280 |
det |
a,structure |
R352 |
T1280 |
T1278 |
pobj |
structure,by |
R353 |
T1281 |
T1280 |
amod |
bipartite,structure |
R354 |
T1282 |
T1280 |
prep |
with,structure |
R355 |
T1283 |
T1284 |
det |
a,domain |
R356 |
T1284 |
T1282 |
pobj |
domain,with |
R357 |
T1285 |
T1284 |
amod |
conserved,domain |
R358 |
T1286 |
T1287 |
npadvmod |
C,terminal |
R359 |
T1287 |
T1284 |
amod |
terminal,domain |
R360 |
T1288 |
T1287 |
punct |
-,terminal |
R361 |
T1289 |
T1284 |
compound |
core,domain |
R362 |
T1290 |
T1284 |
cc |
and,domain |
R363 |
T1291 |
T1292 |
det |
a,domain |
R364 |
T1292 |
T1284 |
conj |
domain,domain |
R365 |
T1293 |
T1292 |
amod |
unique,domain |
R366 |
T1294 |
T1295 |
npadvmod |
N,terminal |
R367 |
T1295 |
T1292 |
amod |
terminal,domain |
R368 |
T1296 |
T1295 |
punct |
-,terminal |
R369 |
T1297 |
T1298 |
dep |
that,varies |
R370 |
T1298 |
T1292 |
relcl |
varies,domain |
R371 |
T1299 |
T1298 |
prep |
in,varies |
R372 |
T1300 |
T1299 |
pobj |
length,in |
R373 |
T1301 |
T1300 |
cc |
and,length |
R374 |
T1302 |
T1303 |
compound |
amino,acid |
R375 |
T1303 |
T1304 |
compound |
acid,composition |
R376 |
T1304 |
T1300 |
conj |
composition,length |
R377 |
T1305 |
T1276 |
punct |
.,characterized |
R378 |
T1307 |
T1308 |
det |
The,domain |
R379 |
T1308 |
T1312 |
nsubjpass |
domain,formed |
R380 |
T1309 |
T1310 |
npadvmod |
C,terminal |
R381 |
T1310 |
T1308 |
amod |
terminal,domain |
R382 |
T1311 |
T1310 |
punct |
-,terminal |
R383 |
T1313 |
T1312 |
auxpass |
is,formed |
R384 |
T1314 |
T1312 |
prep |
by,formed |
R385 |
T1315 |
T1316 |
preconj |
either,repeat |
R386 |
T1316 |
T1314 |
pobj |
repeat,by |
R387 |
T1317 |
T1316 |
det |
a,repeat |
R388 |
T1318 |
T1316 |
nummod |
four,repeat |
R389 |
T1319 |
T1318 |
punct |
-,four |
R390 |
T1320 |
T1318 |
cc |
or,four |
R391 |
T1321 |
T1318 |
conj |
eightfold,four |
R392 |
T1322 |
T1316 |
prep |
of,repeat |
R393 |
T1323 |
T1324 |
advmod |
approximately,70 |
R394 |
T1324 |
T1325 |
nummod |
70,acids |
R395 |
T1325 |
T1322 |
pobj |
acids,of |
R396 |
T1326 |
T1325 |
compound |
amino,acids |
R397 |
T1327 |
T1316 |
punct |
", ",repeat |
R398 |
T1328 |
T1329 |
det |
each,repeat |
R399 |
T1329 |
T1316 |
npadvmod |
repeat,repeat |
R400 |
T1330 |
T1329 |
acl |
carrying,repeat |
R401 |
T1331 |
T1332 |
det |
a,site |
R402 |
T1332 |
T1330 |
dobj |
site,carrying |
R403 |
T1333 |
T1332 |
nmod |
Ca2+,site |
R404 |
T1334 |
T1333 |
punct |
-,Ca2+ |
R405 |
T1335 |
T1333 |
amod |
binding,Ca2+ |
R406 |
T1336 |
T1312 |
punct |
", ",formed |
R407 |
T1337 |
T1312 |
cc |
and,formed |
R408 |
T1338 |
T1312 |
conj |
is,formed |
R409 |
T1339 |
T1338 |
acomp |
responsible,is |
R410 |
T1340 |
T1339 |
prep |
for,responsible |
R411 |
T1341 |
T1342 |
compound |
phospholipid,binding |
R412 |
T1342 |
T1340 |
pobj |
binding,for |
R413 |
T1343 |
T1312 |
punct |
.,formed |
R414 |
T1345 |
T1346 |
det |
The,regions |
R415 |
T1346 |
T1350 |
nsubjpass |
regions,thought |
R416 |
T1347 |
T1348 |
npadvmod |
N,terminal |
R417 |
T1348 |
T1346 |
amod |
terminal,regions |
R418 |
T1349 |
T1348 |
punct |
-,terminal |
R419 |
T1351 |
T1350 |
auxpass |
are,thought |
R420 |
T1352 |
T1353 |
aux |
to,confer |
R421 |
T1353 |
T1350 |
xcomp |
confer,thought |
R422 |
T1354 |
T1355 |
amod |
functional,diversity |
R423 |
T1355 |
T1353 |
dobj |
diversity,confer |
R424 |
T1356 |
T1353 |
prep |
to,confer |
R425 |
T1357 |
T1358 |
det |
the,proteins |
R426 |
T1358 |
T1356 |
pobj |
proteins,to |
R427 |
T1359 |
T1358 |
compound |
annexin,proteins |
R428 |
T1360 |
T1361 |
punct |
[,2 |
R429 |
T1361 |
T1350 |
parataxis |
2,thought |
R430 |
T1362 |
T1361 |
punct |
],2 |
R431 |
T1363 |
T1350 |
punct |
.,thought |
R432 |
T1365 |
T1366 |
det |
The,features |
R433 |
T1366 |
T1368 |
nsubjpass |
features,analyzed |
R434 |
T1367 |
T1366 |
amod |
biochemical,features |
R435 |
T1369 |
T1370 |
advmod |
in,vitro |
R436 |
T1370 |
T1366 |
advmod |
vitro,features |
R437 |
T1371 |
T1368 |
auxpass |
were,analyzed |
R438 |
T1372 |
T1368 |
advmod |
extensively,analyzed |
R439 |
T1373 |
T1368 |
punct |
", ",analyzed |
R440 |
T1374 |
T1368 |
cc |
but,analyzed |
R441 |
T1375 |
T1376 |
det |
the,functions |
R442 |
T1376 |
T1379 |
nsubj |
functions,remain |
R443 |
T1377 |
T1378 |
advmod |
in,vivo |
R444 |
T1378 |
T1376 |
amod |
vivo,functions |
R445 |
T1379 |
T1368 |
conj |
remain,analyzed |
R446 |
T1380 |
T1376 |
prep |
of,functions |
R447 |
T1381 |
T1380 |
pobj |
annexins,of |
R448 |
T1382 |
T1379 |
acomp |
unclear,remain |
R449 |
T1383 |
T1379 |
punct |
.,remain |
R450 |
T1385 |
T1386 |
compound |
Annexin,A7 |
R451 |
T1386 |
T1387 |
nsubjpass |
A7,isolated |
R452 |
T1388 |
T1386 |
punct |
", ",A7 |
R453 |
T1389 |
T1390 |
det |
the,annexin |
R454 |
T1390 |
T1386 |
appos |
annexin,A7 |
R455 |
T1391 |
T1390 |
amod |
first,annexin |
R456 |
T1392 |
T1393 |
aux |
to,described |
R457 |
T1393 |
T1390 |
advcl |
described,annexin |
R458 |
T1394 |
T1393 |
auxpass |
be,described |
R459 |
T1395 |
T1387 |
punct |
", ",isolated |
R460 |
T1396 |
T1387 |
auxpass |
was,isolated |
R461 |
T1397 |
T1387 |
prep |
as,isolated |
R462 |
T1398 |
T1399 |
det |
the,agent |
R463 |
T1399 |
T1397 |
pobj |
agent,as |
R464 |
T1400 |
T1401 |
dep |
that,mediated |
R465 |
T1401 |
T1399 |
relcl |
mediated,agent |
R466 |
T1402 |
T1401 |
dobj |
aggregation,mediated |
R467 |
T1403 |
T1402 |
prep |
of,aggregation |
R468 |
T1404 |
T1405 |
compound |
chromaffin,granules |
R469 |
T1405 |
T1403 |
pobj |
granules,of |
R470 |
T1406 |
T1402 |
cc |
and,aggregation |
R471 |
T1407 |
T1402 |
conj |
fusion,aggregation |
R472 |
T1408 |
T1407 |
prep |
of,fusion |
R473 |
T1409 |
T1408 |
pobj |
membranes,of |
R474 |
T1410 |
T1409 |
cc |
and,membranes |
R475 |
T1411 |
T1409 |
conj |
phospholipids,membranes |
R476 |
T1412 |
T1401 |
prep |
in,mediated |
R477 |
T1413 |
T1414 |
det |
the,presence |
R478 |
T1414 |
T1412 |
pobj |
presence,in |
R479 |
T1415 |
T1414 |
prep |
of,presence |
R480 |
T1416 |
T1417 |
compound |
Ca2+,ions |
R481 |
T1417 |
T1415 |
pobj |
ions,of |
R482 |
T1418 |
T1417 |
punct |
-,ions |
R483 |
T1419 |
T1420 |
punct |
[,3 |
R484 |
T1420 |
T1387 |
parataxis |
3,isolated |
R485 |
T1421 |
T1420 |
punct |
],3 |
R486 |
T1422 |
T1387 |
punct |
.,isolated |
R487 |
T1424 |
T1425 |
compound |
Expression,studies |
R488 |
T1425 |
T1426 |
nsubj |
studies,demonstrated |
R489 |
T1427 |
T1428 |
det |
the,distribution |
R490 |
T1428 |
T1426 |
dobj |
distribution,demonstrated |
R491 |
T1429 |
T1428 |
prep |
of,distribution |
R492 |
T1430 |
T1431 |
compound |
Annexin,A7 |
R493 |
T1431 |
T1429 |
pobj |
A7,of |
R494 |
T1432 |
T1428 |
prep |
in,distribution |
R495 |
T1433 |
T1434 |
det |
a,variety |
R496 |
T1434 |
T1432 |
pobj |
variety,in |
R497 |
T1435 |
T1434 |
amod |
wide,variety |
R498 |
T1436 |
T1434 |
prep |
of,variety |
R499 |
T1437 |
T1436 |
pobj |
tissues,of |
R500 |
T1438 |
T1437 |
cc |
and,tissues |
R501 |
T1439 |
T1437 |
conj |
cells,tissues |
R502 |
T1440 |
T1441 |
advmod |
mainly,enriched |
R503 |
T1441 |
T1434 |
acl |
enriched,variety |
R504 |
T1442 |
T1441 |
prep |
in,enriched |
R505 |
T1443 |
T1444 |
det |
the,cytosol |
R506 |
T1444 |
T1442 |
pobj |
cytosol,in |
R507 |
T1445 |
T1441 |
prep |
in,enriched |
R508 |
T1446 |
T1447 |
amod |
close,association |
R509 |
T1447 |
T1445 |
pobj |
association,in |
R510 |
T1448 |
T1447 |
prep |
with,association |
R511 |
T1449 |
T1450 |
amod |
membranous,structures |
R512 |
T1450 |
T1448 |
pobj |
structures,with |
R513 |
T1451 |
T1426 |
punct |
", ",demonstrated |
R514 |
T1452 |
T1426 |
cc |
but,demonstrated |
R515 |
T1453 |
T1454 |
nsubjpass |
it,described |
R516 |
T1454 |
T1426 |
conj |
described,demonstrated |
R517 |
T1455 |
T1454 |
auxpass |
was,described |
R518 |
T1456 |
T1454 |
advmod |
also,described |
R519 |
T1457 |
T1454 |
prep |
in,described |
R520 |
T1458 |
T1459 |
det |
the,nucleus |
R521 |
T1459 |
T1457 |
pobj |
nucleus,in |
R522 |
T1460 |
T1459 |
prep |
of,nucleus |
R523 |
T1461 |
T1462 |
amod |
adrenal,cells |
R524 |
T1462 |
T1460 |
pobj |
cells,of |
R525 |
T1463 |
T1462 |
compound |
chromaffin,cells |
R526 |
T1464 |
T1465 |
punct |
[,4 |
R527 |
T1465 |
T1454 |
parataxis |
4,described |
R528 |
T1466 |
T1465 |
punct |
],4 |
R529 |
T1467 |
T1454 |
punct |
.,described |
R530 |
T1469 |
T1470 |
det |
The,presence |
R531 |
T1470 |
T1471 |
nsubj |
presence,gives |
R532 |
T1472 |
T1470 |
prep |
of,presence |
R533 |
T1473 |
T1474 |
det |
an,exon |
R534 |
T1474 |
T1472 |
pobj |
exon,of |
R535 |
T1475 |
T1476 |
advmod |
alternatively,spliced |
R536 |
T1476 |
T1474 |
amod |
spliced,exon |
R537 |
T1477 |
T1474 |
compound |
cassette,exon |
R538 |
T1478 |
T1471 |
dobj |
rise,gives |
R539 |
T1479 |
T1471 |
prep |
to,gives |
R540 |
T1480 |
T1481 |
nummod |
two,isoforms |
R541 |
T1481 |
T1479 |
pobj |
isoforms,to |
R542 |
T1482 |
T1483 |
compound |
Annexin,A7 |
R543 |
T1483 |
T1481 |
compound |
A7,isoforms |
R544 |
T1484 |
T1481 |
acl |
corresponding,isoforms |
R545 |
T1485 |
T1484 |
prep |
in,corresponding |
R546 |
T1486 |
T1487 |
amod |
molecular,mass |
R547 |
T1487 |
T1485 |
pobj |
mass,in |
R548 |
T1488 |
T1484 |
prep |
to,corresponding |
R549 |
T1489 |
T1490 |
nummod |
47,kDa |
R550 |
T1490 |
T1488 |
pobj |
kDa,to |
R551 |
T1491 |
T1490 |
cc |
and,kDa |
R552 |
T1492 |
T1493 |
nummod |
51,kDa |
R553 |
T1493 |
T1490 |
conj |
kDa,kDa |
R554 |
T1494 |
T1471 |
punct |
.,gives |
R555 |
T1496 |
T1497 |
det |
The,isoforms |
R556 |
T1497 |
T1498 |
nsubj |
isoforms,differ |
R557 |
T1499 |
T1498 |
prep |
in,differ |
R558 |
T1500 |
T1501 |
poss |
their,domain |
R559 |
T1501 |
T1499 |
pobj |
domain,in |
R560 |
T1502 |
T1503 |
npadvmod |
N,terminal |
R561 |
T1503 |
T1501 |
amod |
terminal,domain |
R562 |
T1504 |
T1503 |
punct |
-,terminal |
R563 |
T1505 |
T1498 |
cc |
and,differ |
R564 |
T1506 |
T1498 |
conj |
exhibit,differ |
R565 |
T1507 |
T1508 |
det |
a,pattern |
R566 |
T1508 |
T1506 |
dobj |
pattern,exhibit |
R567 |
T1509 |
T1510 |
npadvmod |
tissue,specific |
R568 |
T1510 |
T1508 |
amod |
specific,pattern |
R569 |
T1511 |
T1510 |
punct |
-,specific |
R570 |
T1512 |
T1508 |
compound |
expression,pattern |
R571 |
T1513 |
T1498 |
punct |
.,differ |
R572 |
T1515 |
T1516 |
det |
The,isoform |
R573 |
T1516 |
T1519 |
nsubj |
isoform,is |
R574 |
T1517 |
T1518 |
nummod |
47,kDa |
R575 |
T1518 |
T1516 |
compound |
kDa,isoform |
R576 |
T1520 |
T1519 |
acomp |
present,is |
R577 |
T1521 |
T1519 |
prep |
in,is |
R578 |
T1522 |
T1523 |
det |
all,tissues |
R579 |
T1523 |
T1521 |
pobj |
tissues,in |
R580 |
T1524 |
T1523 |
prep |
except,tissues |
R581 |
T1525 |
T1524 |
prep |
for,except |
R582 |
T1526 |
T1527 |
amod |
skeletal,muscle |
R583 |
T1527 |
T1525 |
pobj |
muscle,for |
R584 |
T1528 |
T1527 |
punct |
", ",muscle |
R585 |
T1529 |
T1530 |
advmod |
where,is |
R586 |
T1530 |
T1527 |
relcl |
is,muscle |
R587 |
T1531 |
T1532 |
det |
the,isoform |
R588 |
T1532 |
T1530 |
nsubj |
isoform,is |
R589 |
T1533 |
T1534 |
nummod |
51,kDa |
R590 |
T1534 |
T1532 |
compound |
kDa,isoform |
R591 |
T1535 |
T1530 |
advmod |
exclusively,is |
R592 |
T1536 |
T1530 |
acomp |
present,is |
R593 |
T1537 |
T1519 |
punct |
.,is |
R594 |
T1539 |
T1540 |
compound |
Heart,muscle |
R595 |
T1540 |
T1541 |
nsubj |
muscle,contain |
R596 |
T1542 |
T1540 |
punct |
", ",muscle |
R597 |
T1543 |
T1544 |
compound |
brain,tissue |
R598 |
T1544 |
T1540 |
conj |
tissue,muscle |
R599 |
T1545 |
T1544 |
cc |
and,tissue |
R600 |
T1546 |
T1547 |
amod |
red,cells |
R601 |
T1547 |
T1544 |
conj |
cells,tissue |
R602 |
T1548 |
T1547 |
compound |
blood,cells |
R603 |
T1549 |
T1550 |
det |
both,isoforms |
R604 |
T1550 |
T1541 |
dobj |
isoforms,contain |
R605 |
T1551 |
T1552 |
punct |
[,5 |
R606 |
T1552 |
T1541 |
parataxis |
5,contain |
R607 |
T1553 |
T1554 |
punct |
-,8 |
R608 |
T1554 |
T1552 |
prep |
8,5 |
R609 |
T1555 |
T1552 |
punct |
],5 |
R610 |
T1556 |
T1541 |
punct |
.,contain |
R611 |
T1558 |
T1559 |
amod |
Previous,studies |
R612 |
T1559 |
T1560 |
nsubj |
studies,indicated |
R613 |
T1561 |
T1562 |
mark |
that,changes |
R614 |
T1562 |
T1560 |
ccomp |
changes,indicated |
R615 |
T1563 |
T1564 |
det |
the,localization |
R616 |
T1564 |
T1562 |
nsubj |
localization,changes |
R617 |
T1565 |
T1564 |
amod |
subcellular,localization |
R618 |
T1566 |
T1564 |
prep |
of,localization |
R619 |
T1567 |
T1568 |
compound |
Annexin,A7 |
R620 |
T1568 |
T1566 |
pobj |
A7,of |
R621 |
T1569 |
T1562 |
prep |
during,changes |
R622 |
T1570 |
T1571 |
compound |
myoblast,differentiation |
R623 |
T1571 |
T1569 |
pobj |
differentiation,during |
R624 |
T1572 |
T1560 |
punct |
.,indicated |
R625 |
T1574 |
T1575 |
prep |
In,distributed |
R626 |
T1576 |
T1577 |
amod |
undifferentiated,cells |
R627 |
T1577 |
T1574 |
pobj |
cells,In |
R628 |
T1578 |
T1579 |
det |
the,protein |
R629 |
T1579 |
T1575 |
nsubjpass |
protein,distributed |
R630 |
T1580 |
T1575 |
auxpass |
is,distributed |
R631 |
T1581 |
T1575 |
advmod |
equally,distributed |
R632 |
T1582 |
T1575 |
prep |
between,distributed |
R633 |
T1583 |
T1584 |
nmod |
cytosol,fractions |
R634 |
T1584 |
T1582 |
pobj |
fractions,between |
R635 |
T1585 |
T1583 |
cc |
and,cytosol |
R636 |
T1586 |
T1583 |
conj |
membrane,cytosol |
R637 |
T1587 |
T1588 |
mark |
while,is |
R638 |
T1588 |
T1575 |
advcl |
is,distributed |
R639 |
T1589 |
T1588 |
prep |
in,is |
R640 |
T1590 |
T1591 |
amod |
differentiated,cells |
R641 |
T1591 |
T1589 |
pobj |
cells,in |
R642 |
T1592 |
T1588 |
nsubj |
it,is |
R643 |
T1593 |
T1588 |
advmod |
exclusively,is |
R644 |
T1594 |
T1588 |
acomp |
present,is |
R645 |
T1595 |
T1588 |
prep |
in,is |
R646 |
T1596 |
T1597 |
det |
the,fraction |
R647 |
T1597 |
T1595 |
pobj |
fraction,in |
R648 |
T1598 |
T1597 |
compound |
membrane,fraction |
R649 |
T1599 |
T1600 |
punct |
[,7 |
R650 |
T1600 |
T1588 |
parataxis |
7,is |
R651 |
T1601 |
T1600 |
punct |
],7 |
R652 |
T1602 |
T1575 |
punct |
.,distributed |
R653 |
T1604 |
T1605 |
nsubj |
Reports,demonstrated |
R654 |
T1606 |
T1604 |
prep |
by,Reports |
R655 |
T1607 |
T1606 |
pobj |
Clemen,by |
R656 |
T1608 |
T1609 |
advmod |
et,al. |
R657 |
T1609 |
T1607 |
advmod |
al.,Clemen |
R658 |
T1610 |
T1611 |
punct |
[,9 |
R659 |
T1611 |
T1607 |
parataxis |
9,Clemen |
R660 |
T1612 |
T1611 |
punct |
],9 |
R661 |
T1613 |
T1607 |
cc |
and,Clemen |
R662 |
T1614 |
T1607 |
conj |
Herr,Clemen |
R663 |
T1615 |
T1616 |
advmod |
et,al. |
R664 |
T1616 |
T1614 |
advmod |
al.,Herr |
R665 |
T1617 |
T1618 |
punct |
[,10 |
R666 |
T1618 |
T1614 |
parataxis |
10,Herr |
R667 |
T1619 |
T1618 |
nummod |
8,10 |
R668 |
T1620 |
T1618 |
punct |
",",10 |
R669 |
T1621 |
T1618 |
punct |
],10 |
R670 |
T1622 |
T1605 |
dobj |
roles,demonstrated |
R671 |
T1623 |
T1622 |
prep |
for,roles |
R672 |
T1624 |
T1625 |
compound |
Annexin,A7 |
R673 |
T1625 |
T1623 |
pobj |
A7,for |
R674 |
T1626 |
T1622 |
prep |
in,roles |
R675 |
T1627 |
T1626 |
pobj |
shape,in |
R676 |
T1628 |
T1627 |
cc |
and,shape |
R677 |
T1629 |
T1630 |
amod |
osmotic,resistance |
R678 |
T1630 |
T1627 |
conj |
resistance,shape |
R679 |
T1631 |
T1627 |
prep |
of,shape |
R680 |
T1632 |
T1633 |
amod |
red,cells |
R681 |
T1633 |
T1631 |
pobj |
cells,of |
R682 |
T1634 |
T1633 |
compound |
blood,cells |
R683 |
T1635 |
T1627 |
punct |
", ",shape |
R684 |
T1636 |
T1637 |
compound |
platelet,velocity |
R685 |
T1637 |
T1627 |
appos |
velocity,shape |
R686 |
T1638 |
T1637 |
compound |
aggregation,velocity |
R687 |
T1639 |
T1626 |
punct |
", ",in |
R688 |
T1640 |
T1626 |
cc |
and,in |
R689 |
T1641 |
T1626 |
conj |
in,in |
R690 |
T1642 |
T1643 |
det |
the,velocity |
R691 |
T1643 |
T1641 |
pobj |
velocity,in |
R692 |
T1644 |
T1643 |
prep |
of,velocity |
R693 |
T1645 |
T1646 |
amod |
spreading,waves |
R694 |
T1646 |
T1644 |
pobj |
waves,of |
R695 |
T1647 |
T1646 |
amod |
astrocytic,waves |
R696 |
T1648 |
T1646 |
compound |
Ca2+,waves |
R697 |
T1649 |
T1646 |
punct |
-,waves |
R698 |
T1650 |
T1605 |
punct |
.,demonstrated |
R699 |
T1652 |
T1653 |
compound |
Annexin,A7 |
R700 |
T1653 |
T1654 |
nsubjpass |
A7,involved |
R701 |
T1655 |
T1654 |
auxpass |
is,involved |
R702 |
T1656 |
T1654 |
advmod |
also,involved |
R703 |
T1657 |
T1654 |
prep |
in,involved |
R704 |
T1658 |
T1659 |
det |
the,maintenance |
R705 |
T1659 |
T1657 |
pobj |
maintenance,in |
R706 |
T1660 |
T1659 |
prep |
of,maintenance |
R707 |
T1661 |
T1662 |
amod |
regular,electrophysiology |
R708 |
T1662 |
T1660 |
pobj |
electrophysiology,of |
R709 |
T1663 |
T1662 |
amod |
cardiac,electrophysiology |
R710 |
T1664 |
T1662 |
cc |
and,electrophysiology |
R711 |
T1665 |
T1666 |
compound |
Ca2+,homeostasis |
R712 |
T1666 |
T1662 |
conj |
homeostasis,electrophysiology |
R713 |
T1667 |
T1666 |
punct |
-,homeostasis |
R714 |
T1668 |
T1669 |
punct |
[,Schrickel |
R715 |
T1669 |
T1654 |
meta |
Schrickel,involved |
R716 |
T1670 |
T1669 |
nmod |
et,Schrickel |
R717 |
T1671 |
T1669 |
nmod |
al.,Schrickel |
R718 |
T1672 |
T1669 |
punct |
", ",Schrickel |
R719 |
T1673 |
T1669 |
amod |
submitted,Schrickel |
R720 |
T1674 |
T1669 |
punct |
],Schrickel |
R721 |
T1675 |
T1654 |
punct |
.,involved |
R722 |
T1677 |
T1678 |
amod |
Detailed,reports |
R723 |
T1678 |
T1679 |
nsubj |
reports,are |
R724 |
T1680 |
T1678 |
prep |
on,reports |
R725 |
T1681 |
T1680 |
pobj |
appearance,on |
R726 |
T1682 |
T1681 |
cc |
and,appearance |
R727 |
T1683 |
T1681 |
conj |
distribution,appearance |
R728 |
T1684 |
T1681 |
prep |
of,appearance |
R729 |
T1685 |
T1686 |
compound |
Annexin,A7 |
R730 |
T1686 |
T1684 |
pobj |
A7,of |
R731 |
T1687 |
T1681 |
prep |
during,appearance |
R732 |
T1688 |
T1689 |
compound |
brain,development |
R733 |
T1689 |
T1687 |
pobj |
development,during |
R734 |
T1690 |
T1679 |
neg |
not,are |
R735 |
T1691 |
T1679 |
acomp |
available,are |
R736 |
T1692 |
T1679 |
punct |
.,are |
R737 |
T1694 |
T1695 |
prep |
In,focus |
R738 |
T1696 |
T1697 |
det |
the,study |
R739 |
T1697 |
T1694 |
pobj |
study,In |
R740 |
T1698 |
T1697 |
amod |
present,study |
R741 |
T1699 |
T1695 |
nsubj |
we,focus |
R742 |
T1700 |
T1695 |
prep |
on,focus |
R743 |
T1701 |
T1702 |
det |
the,distribution |
R744 |
T1702 |
T1700 |
pobj |
distribution,on |
R745 |
T1703 |
T1702 |
prep |
of,distribution |
R746 |
T1704 |
T1705 |
compound |
Annexin,A7 |
R747 |
T1705 |
T1703 |
pobj |
A7,of |
R748 |
T1706 |
T1702 |
prep |
in,distribution |
R749 |
T1707 |
T1708 |
det |
the,brain |
R750 |
T1708 |
T1706 |
pobj |
brain,in |
R751 |
T1709 |
T1708 |
amod |
developing,brain |
R752 |
T1710 |
T1708 |
prep |
of,brain |
R753 |
T1711 |
T1712 |
compound |
mice,embryos |
R754 |
T1712 |
T1710 |
pobj |
embryos,of |
R755 |
T1713 |
T1712 |
prep |
between,embryos |
R756 |
T1714 |
T1713 |
pobj |
E5,between |
R757 |
T1715 |
T1714 |
cc |
and,E5 |
R758 |
T1716 |
T1714 |
conj |
E16,E5 |
R759 |
T1717 |
T1706 |
punct |
", ",in |
R760 |
T1718 |
T1706 |
cc |
and,in |
R761 |
T1719 |
T1706 |
conj |
in,in |
R762 |
T1720 |
T1721 |
det |
the,brain |
R763 |
T1721 |
T1719 |
pobj |
brain,in |
R764 |
T1722 |
T1721 |
amod |
adult,brain |
R765 |
T1723 |
T1721 |
compound |
mouse,brain |
R766 |
T1724 |
T1695 |
punct |
.,focus |
R771 |
T2289 |
T2290 |
compound |
Annexin,A7 |
R772 |
T2290 |
T2291 |
nsubjpass |
A7,expressed |
R773 |
T2292 |
T2291 |
auxpass |
is,expressed |
R774 |
T2293 |
T2291 |
prep |
in,expressed |
R775 |
T2294 |
T2295 |
det |
the,embryo |
R776 |
T2295 |
T2293 |
pobj |
embryo,in |
R777 |
T2296 |
T2295 |
amod |
early,embryo |
R778 |
T2297 |
T2295 |
compound |
mouse,embryo |
R779 |
T2299 |
T2300 |
advmod |
First,examined |
R780 |
T2301 |
T2300 |
nsubj |
we,examined |
R781 |
T2302 |
T2303 |
det |
the,expression |
R782 |
T2303 |
T2300 |
dobj |
expression,examined |
R783 |
T2304 |
T2303 |
prep |
of,expression |
R784 |
T2305 |
T2306 |
compound |
Annexin,A7 |
R785 |
T2306 |
T2304 |
pobj |
A7,of |
R786 |
T2307 |
T2303 |
prep |
in,expression |
R787 |
T2308 |
T2309 |
compound |
ES,cells |
R788 |
T2309 |
T2307 |
pobj |
cells,in |
R789 |
T2310 |
T2311 |
punct |
(,Bruce4 |
R790 |
T2311 |
T2309 |
parataxis |
Bruce4,cells |
R791 |
T2312 |
T2311 |
punct |
", ",Bruce4 |
R792 |
T2313 |
T2309 |
acl |
established,cells |
R793 |
T2314 |
T2313 |
prep |
from,established |
R794 |
T2315 |
T2316 |
compound |
C57BL,6J |
R795 |
T2316 |
T2318 |
compound |
6J,mice |
R796 |
T2317 |
T2316 |
punct |
/,6J |
R797 |
T2318 |
T2314 |
pobj |
mice,from |
R798 |
T2319 |
T2309 |
punct |
),cells |
R799 |
T2320 |
T2309 |
cc |
and,cells |
R800 |
T2321 |
T2322 |
det |
the,stages |
R801 |
T2322 |
T2309 |
conj |
stages,cells |
R802 |
T2323 |
T2322 |
amod |
early,stages |
R803 |
T2324 |
T2322 |
prep |
of,stages |
R804 |
T2325 |
T2326 |
nmod |
mouse,development |
R805 |
T2326 |
T2324 |
pobj |
development,of |
R806 |
T2327 |
T2326 |
amod |
embryonic,development |
R807 |
T2328 |
T2300 |
prep |
at,examined |
R808 |
T2329 |
T2330 |
det |
the,level |
R809 |
T2330 |
T2328 |
pobj |
level,at |
R810 |
T2331 |
T2330 |
compound |
mRNA,level |
R811 |
T2332 |
T2300 |
prep |
by,examined |
R812 |
T2333 |
T2334 |
compound |
northern,blot |
R813 |
T2334 |
T2335 |
compound |
blot,analysis |
R814 |
T2335 |
T2332 |
pobj |
analysis,by |
R815 |
T2336 |
T2300 |
cc |
and,examined |
R816 |
T2337 |
T2300 |
conj |
at,examined |
R817 |
T2338 |
T2339 |
det |
the,level |
R818 |
T2339 |
T2337 |
pobj |
level,at |
R819 |
T2340 |
T2339 |
compound |
protein,level |
R820 |
T2341 |
T2337 |
prep |
by,at |
R821 |
T2342 |
T2343 |
compound |
Western,blotting |
R822 |
T2343 |
T2341 |
pobj |
blotting,by |
R823 |
T2344 |
T2343 |
cc |
and,blotting |
R824 |
T2345 |
T2343 |
conj |
immunohistochemistry,blotting |
R825 |
T2346 |
T2337 |
punct |
", ",at |
R826 |
T2347 |
T2337 |
conj |
respectively,at |
R827 |
T2348 |
T2300 |
punct |
.,examined |
R828 |
T2350 |
T2351 |
compound |
Northern,blot |
R829 |
T2351 |
T2352 |
compound |
blot,analysis |
R830 |
T2352 |
T2353 |
nsubj |
analysis,shows |
R831 |
T2354 |
T2353 |
prep |
in,shows |
R832 |
T2355 |
T2356 |
compound |
ES,cells |
R833 |
T2356 |
T2354 |
pobj |
cells,in |
R834 |
T2357 |
T2356 |
punct |
-,cells |
R835 |
T2358 |
T2354 |
cc |
and,in |
R836 |
T2359 |
T2354 |
conj |
at,in |
R837 |
T2360 |
T2361 |
amod |
embryonic,E7 |
R838 |
T2361 |
T2359 |
pobj |
E7,at |
R839 |
T2362 |
T2361 |
compound |
days,E7 |
R840 |
T2363 |
T2361 |
punct |
", ",E7 |
R841 |
T2364 |
T2361 |
conj |
E11,E7 |
R842 |
T2365 |
T2364 |
punct |
", ",E11 |
R843 |
T2366 |
T2364 |
conj |
E15,E11 |
R844 |
T2367 |
T2366 |
punct |
", ",E15 |
R845 |
T2368 |
T2366 |
cc |
and,E15 |
R846 |
T2369 |
T2366 |
conj |
E17,E15 |
R847 |
T2370 |
T2371 |
nummod |
two,species |
R848 |
T2371 |
T2353 |
dobj |
species,shows |
R849 |
T2372 |
T2371 |
compound |
mRNA,species |
R850 |
T2373 |
T2371 |
prep |
of,species |
R851 |
T2374 |
T2375 |
nummod |
1.8,kb |
R852 |
T2375 |
T2373 |
pobj |
kb,of |
R853 |
T2376 |
T2375 |
cc |
and,kb |
R854 |
T2377 |
T2378 |
nummod |
2.4,kb |
R855 |
T2378 |
T2375 |
conj |
kb,kb |
R856 |
T2379 |
T2371 |
punct |
", ",species |
R857 |
T2380 |
T2381 |
dep |
which,result |
R858 |
T2381 |
T2371 |
relcl |
result,species |
R859 |
T2382 |
T2381 |
prep |
from,result |
R860 |
T2383 |
T2384 |
amod |
alternative,splicing |
R861 |
T2384 |
T2382 |
pobj |
splicing,from |
R862 |
T2385 |
T2381 |
prep |
in,result |
R863 |
T2386 |
T2387 |
det |
the,end |
R864 |
T2387 |
T2385 |
pobj |
end,in |
R865 |
T2388 |
T2387 |
amod |
untranslated,end |
R866 |
T2389 |
T2387 |
nummod |
3,end |
R867 |
T2390 |
T2389 |
punct |
',3 |
R868 |
T2391 |
T2392 |
punct |
(,Fig. |
R869 |
T2392 |
T2353 |
parataxis |
Fig.,shows |
R870 |
T2393 |
T2392 |
nummod |
1A,Fig. |
R871 |
T2394 |
T2392 |
punct |
),Fig. |
R872 |
T2395 |
T2396 |
punct |
[,11 |
R873 |
T2396 |
T2353 |
parataxis |
11,shows |
R874 |
T2397 |
T2396 |
punct |
],11 |
R875 |
T2398 |
T2353 |
punct |
.,shows |
R876 |
T2400 |
T2401 |
nsubj |
We,found |
R877 |
T2402 |
T2403 |
amod |
similar,levels |
R878 |
T2403 |
T2401 |
dobj |
levels,found |
R879 |
T2404 |
T2405 |
compound |
Annexin,A7 |
R880 |
T2405 |
T2403 |
compound |
A7,levels |
R881 |
T2406 |
T2403 |
compound |
mRNA,levels |
R882 |
T2407 |
T2401 |
prep |
in,found |
R883 |
T2408 |
T2409 |
det |
the,stages |
R884 |
T2409 |
T2407 |
pobj |
stages,in |
R885 |
T2410 |
T2409 |
nummod |
four,stages |
R886 |
T2411 |
T2409 |
amod |
embryonic,stages |
R887 |
T2412 |
T2401 |
punct |
.,found |
R888 |
T2414 |
T2415 |
nsubj |
Reprobing,verified |
R889 |
T2415 |
T2422 |
ccomp |
verified,is |
R890 |
T2416 |
T2414 |
prep |
with,Reprobing |
R891 |
T2417 |
T2418 |
det |
a,probe |
R892 |
T2418 |
T2416 |
pobj |
probe,with |
R893 |
T2419 |
T2420 |
compound |
β,actin |
R894 |
T2420 |
T2418 |
compound |
actin,probe |
R895 |
T2421 |
T2420 |
punct |
-,actin |
R896 |
T2423 |
T2424 |
amod |
equal,loading |
R897 |
T2424 |
T2415 |
dobj |
loading,verified |
R898 |
T2425 |
T2422 |
punct |
;,is |
R899 |
T2426 |
T2427 |
det |
the,appearance |
R900 |
T2427 |
T2422 |
nsubj |
appearance,is |
R901 |
T2428 |
T2427 |
prep |
of,appearance |
R902 |
T2429 |
T2430 |
det |
a,species |
R903 |
T2430 |
T2428 |
pobj |
species,of |
R904 |
T2431 |
T2432 |
amod |
faster,migrating |
R905 |
T2432 |
T2430 |
amod |
migrating,species |
R906 |
T2433 |
T2430 |
compound |
mRNA,species |
R907 |
T2434 |
T2430 |
prep |
in,species |
R908 |
T2435 |
T2434 |
pobj |
addition,in |
R909 |
T2436 |
T2435 |
prep |
to,addition |
R910 |
T2437 |
T2438 |
det |
the,species |
R911 |
T2438 |
T2436 |
pobj |
species,to |
R912 |
T2439 |
T2440 |
nummod |
2.0,kb |
R913 |
T2440 |
T2438 |
compound |
kb,species |
R914 |
T2441 |
T2422 |
acomp |
characteristic,is |
R915 |
T2442 |
T2422 |
prep |
for,is |
R916 |
T2443 |
T2444 |
compound |
β,actin |
R917 |
T2444 |
T2442 |
pobj |
actin,for |
R918 |
T2445 |
T2444 |
punct |
-,actin |
R919 |
T2446 |
T2447 |
punct |
[,12 |
R920 |
T2447 |
T2422 |
parataxis |
12,is |
R921 |
T2448 |
T2447 |
punct |
],12 |
R922 |
T2449 |
T2422 |
punct |
.,is |
R923 |
T2451 |
T2452 |
prep |
On,express |
R992 |
T2525 |
T2526 |
det |
the,stage |
R993 |
T2526 |
T2524 |
pobj |
stage,of |
R994 |
T2527 |
T2526 |
compound |
cylinder,stage |
R995 |
T2528 |
T2501 |
prep |
at,observed |
R996 |
T2529 |
T2528 |
pobj |
E5,at |
R997 |
T2530 |
T2501 |
prep |
with,observed |
R998 |
T2531 |
T2532 |
det |
a,expression |
R999 |
T2532 |
T2530 |
pobj |
expression,with |
R1000 |
T2533 |
T2532 |
amod |
weaker,expression |
R1001 |
T2534 |
T2535 |
compound |
Annexin,A7 |
R1002 |
T2535 |
T2532 |
compound |
A7,expression |
R1003 |
T2536 |
T2532 |
prep |
in,expression |
R1004 |
T2537 |
T2538 |
det |
the,ectoderm |
R1005 |
T2538 |
T2536 |
pobj |
ectoderm,in |
R1006 |
T2539 |
T2540 |
punct |
(,2A |
R1007 |
T2540 |
T2501 |
parataxis |
2A,observed |
R1008 |
T2541 |
T2540 |
compound |
Fig.,2A |
R1009 |
T2542 |
T2543 |
punct |
–,C |
R1010 |
T2543 |
T2540 |
prep |
C,2A |
R1011 |
T2544 |
T2540 |
punct |
),2A |
R1012 |
T2545 |
T2501 |
punct |
.,observed |
R1013 |
T2547 |
T2548 |
prep |
At,detected |
R1014 |
T2549 |
T2547 |
pobj |
E8,At |
R1015 |
T2550 |
T2551 |
compound |
Annexin,A7 |
R1016 |
T2551 |
T2548 |
nsubjpass |
A7,detected |
R1017 |
T2552 |
T2548 |
aux |
can,detected |
R1018 |
T2553 |
T2548 |
auxpass |
be,detected |
R1019 |
T2554 |
T2548 |
prep |
in,detected |
R1020 |
T2555 |
T2556 |
advmod |
almost,tissues |
R1021 |
T2556 |
T2554 |
pobj |
tissues,in |
R1022 |
T2557 |
T2556 |
det |
all,tissues |
R1023 |
T2558 |
T2556 |
prep |
of,tissues |
R1024 |
T2559 |
T2560 |
det |
the,embryo |
R1025 |
T2560 |
T2558 |
pobj |
embryo,of |
R1026 |
T2561 |
T2562 |
punct |
(,shown |
R1027 |
T2562 |
T2548 |
parataxis |
shown,detected |
R1028 |
T2563 |
T2562 |
nsubj |
data,shown |
R1029 |
T2564 |
T2562 |
neg |
not,shown |
R1030 |
T2565 |
T2562 |
punct |
),shown |
R1031 |
T2566 |
T2548 |
punct |
.,detected |
R1032 |
T2568 |
T2569 |
det |
A,correlation |
R1033 |
T2569 |
T2570 |
nsubj |
correlation,is |
R1034 |
T2571 |
T2569 |
prep |
between,correlation |
R1035 |
T2572 |
T2573 |
det |
the,presence |
R1036 |
T2573 |
T2571 |
pobj |
presence,between |
R1037 |
T2574 |
T2573 |
prep |
of,presence |
R1038 |
T2575 |
T2576 |
compound |
Annexin,A7 |
R1039 |
T2576 |
T2574 |
pobj |
A7,of |
R1040 |
T2577 |
T2573 |
cc |
and,presence |
R1041 |
T2578 |
T2579 |
det |
the,origin |
R1042 |
T2579 |
T2573 |
conj |
origin,presence |
R1043 |
T2580 |
T2579 |
prep |
of,origin |
R1044 |
T2581 |
T2582 |
det |
a,type |
R1045 |
T2582 |
T2580 |
pobj |
type,of |
R1046 |
T2583 |
T2582 |
compound |
cell,type |
R1047 |
T2584 |
T2582 |
prep |
from,type |
R1048 |
T2585 |
T2586 |
quantmod |
one,three |
R1049 |
T2586 |
T2589 |
nummod |
three,layers |
R1050 |
T2587 |
T2586 |
quantmod |
of,three |
R1051 |
T2588 |
T2586 |
quantmod |
the,three |
R1052 |
T2589 |
T2584 |
pobj |
layers,from |
R1053 |
T2590 |
T2589 |
compound |
germ,layers |
R1054 |
T2591 |
T2570 |
neg |
not,is |
R1055 |
T2592 |
T2570 |
acomp |
apparent,is |
R1056 |
T2593 |
T2570 |
punct |
.,is |
R1057 |
T2595 |
T2596 |
nsubj |
We,noted |
R1058 |
T2597 |
T2596 |
advmod |
specifically,noted |
R1059 |
T2598 |
T2599 |
det |
the,localization |
R1060 |
T2599 |
T2596 |
dobj |
localization,noted |
R1061 |
T2600 |
T2599 |
prep |
of,localization |
R1062 |
T2601 |
T2602 |
det |
the,protein |
R1063 |
T2602 |
T2600 |
pobj |
protein,of |
R1064 |
T2603 |
T2596 |
prep |
in,noted |
R1065 |
T2604 |
T2605 |
det |
the,neuroepithelium |
R1066 |
T2605 |
T2603 |
pobj |
neuroepithelium,in |
R1067 |
T2606 |
T2605 |
prep |
of,neuroepithelium |
R1068 |
T2607 |
T2608 |
det |
the,fold |
R1069 |
T2608 |
T2606 |
pobj |
fold,of |
R1070 |
T2609 |
T2608 |
amod |
neural,fold |
R1071 |
T2610 |
T2608 |
cc |
and,fold |
R1072 |
T2611 |
T2608 |
conj |
tube,fold |
R1073 |
T2612 |
T2613 |
punct |
(,E13 |
R1074 |
T2613 |
T2596 |
parataxis |
E13,noted |
R1075 |
T2614 |
T2615 |
compound |
Fig.,2D |
R1076 |
T2615 |
T2616 |
dep |
2D,E8 |
R1077 |
T2616 |
T2613 |
dep |
E8,E13 |
R1078 |
T2617 |
T2618 |
punct |
–,F |
R1079 |
T2618 |
T2615 |
prep |
F,2D |
R1080 |
T2619 |
T2616 |
punct |
", ",E8 |
R1081 |
T2620 |
T2616 |
amod |
proximal,E8 |
R1082 |
T2621 |
T2616 |
punct |
", ",E8 |
R1083 |
T2622 |
T2613 |
punct |
;,E13 |
R1084 |
T2623 |
T2624 |
compound |
Fig.,2G |
R1085 |
T2624 |
T2613 |
dep |
2G,E13 |
R1086 |
T2625 |
T2626 |
punct |
–,I |
R1087 |
T2626 |
T2624 |
prep |
I,2G |
R1088 |
T2627 |
T2613 |
punct |
", ",E13 |
R1089 |
T2628 |
T2613 |
amod |
distal,E13 |
R1090 |
T2629 |
T2613 |
punct |
", ",E13 |
R1091 |
T2630 |
T2613 |
punct |
),E13 |
R1092 |
T2631 |
T2596 |
punct |
.,noted |
R1093 |
T2633 |
T2634 |
prep |
At,was |
R1094 |
T2635 |
T2636 |
det |
the,level |
R1095 |
T2636 |
T2633 |
pobj |
level,At |
R1096 |
T2637 |
T2636 |
amod |
subcellular,level |
R1097 |
T2638 |
T2634 |
punct |
", ",was |
R1098 |
T2639 |
T2640 |
compound |
Annexin,A7 |
R1099 |
T2640 |
T2634 |
nsubj |
A7,was |
R1100 |
T2641 |
T2634 |
acomp |
present,was |
R1101 |
T2642 |
T2634 |
prep |
in,was |
R1102 |
T2643 |
T2644 |
compound |
punctate,structures |
R1103 |
T2644 |
T2642 |
pobj |
structures,in |
R1104 |
T2645 |
T2646 |
advmod |
mainly,in |
R1105 |
T2646 |
T2634 |
prep |
in,was |
R1106 |
T2647 |
T2648 |
det |
the,cytosol |
R1107 |
T2648 |
T2646 |
pobj |
cytosol,in |
R1108 |
T2649 |
T2648 |
prep |
of,cytosol |
R1109 |
T2650 |
T2651 |
det |
all,cells |
R1110 |
T2651 |
T2649 |
pobj |
cells,of |
R1111 |
T2652 |
T2651 |
amod |
examined,cells |
R1112 |
T2653 |
T2654 |
punct |
(,F |
R1113 |
T2654 |
T2634 |
parataxis |
F,was |
R1114 |
T2655 |
T2654 |
nmod |
Fig.,F |
R1115 |
T2656 |
T2654 |
nmod |
2E,F |
R1116 |
T2657 |
T2654 |
punct |
",",F |
R1117 |
T2658 |
T2654 |
punct |
),F |
R1118 |
T2659 |
T2634 |
punct |
.,was |
R1119 |
T2661 |
T2662 |
det |
This,distribution |
R1120 |
T2662 |
T2663 |
nsubjpass |
distribution,observed |
R1121 |
T2664 |
T2663 |
auxpass |
was,observed |
R1122 |
T2665 |
T2663 |
advmod |
also,observed |
R1123 |
T2666 |
T2663 |
prep |
during,observed |
R1124 |
T2667 |
T2668 |
amod |
subsequent,development |
R1125 |
T2668 |
T2666 |
pobj |
development,during |
R1126 |
T2669 |
T2663 |
punct |
.,observed |
R1127 |
T2973 |
T2974 |
compound |
Annexin,A7 |
R1128 |
T2974 |
T2975 |
nsubj |
A7,changes |
R1129 |
T2976 |
T2977 |
poss |
its,localization |
R1130 |
T2977 |
T2975 |
dobj |
localization,changes |
R1131 |
T2978 |
T2977 |
amod |
subcellular,localization |
R1132 |
T2979 |
T2975 |
prep |
in,changes |
R1133 |
T2980 |
T2981 |
det |
the,brain |
R1134 |
T2981 |
T2979 |
pobj |
brain,in |
R1135 |
T2982 |
T2981 |
amod |
embryonic,brain |
R1136 |
T2983 |
T2975 |
prep |
at,changes |
R1137 |
T2984 |
T2983 |
pobj |
E16,at |
R1138 |
T2986 |
T2987 |
advmod |
Next,analysed |
R1139 |
T2988 |
T2987 |
nsubj |
we,analysed |
R1140 |
T2989 |
T2990 |
det |
the,distribution |
R1141 |
T2990 |
T2987 |
dobj |
distribution,analysed |
R1142 |
T2991 |
T2990 |
amod |
cellular,distribution |
R1143 |
T2992 |
T2991 |
cc |
and,cellular |
R1144 |
T2993 |
T2991 |
conj |
subcellular,cellular |
R1145 |
T2994 |
T2990 |
prep |
of,distribution |
R1146 |
T2995 |
T2996 |
compound |
Annexin,A7 |
R1147 |
T2996 |
T2994 |
pobj |
A7,of |
R1148 |
T2997 |
T2990 |
prep |
in,distribution |
R1149 |
T2998 |
T2999 |
det |
the,brain |
R1150 |
T2999 |
T2997 |
pobj |
brain,in |
R1151 |
T3000 |
T2999 |
amod |
developing,brain |
R1152 |
T3001 |
T2999 |
compound |
mouse,brain |
R1153 |
T3002 |
T3003 |
punct |
(,Fig. |
R1154 |
T3003 |
T2987 |
parataxis |
Fig.,analysed |
R1155 |
T3004 |
T3003 |
nummod |
3,Fig. |
R1156 |
T3005 |
T3003 |
punct |
),Fig. |
R1157 |
T3006 |
T2987 |
punct |
.,analysed |
R1158 |
T3008 |
T3009 |
prep |
At,are |
R1159 |
T3010 |
T3008 |
pobj |
E13,At |
R1160 |
T3011 |
T3012 |
punct |
–,E16 |
R1161 |
T3012 |
T3010 |
prep |
E16,E13 |
R1162 |
T3013 |
T3009 |
prep |
in,are |
R1163 |
T3014 |
T3015 |
amod |
transverse,sections |
R1164 |
T3015 |
T3013 |
pobj |
sections,in |
R1165 |
T3016 |
T3015 |
prep |
of,sections |
R1166 |
T3017 |
T3018 |
det |
the,brain |
R1167 |
T3018 |
T3016 |
pobj |
brain,of |
R1168 |
T3019 |
T3018 |
amod |
embryonic,brain |
R1169 |
T3020 |
T3009 |
punct |
", ",are |
R1170 |
T3021 |
T3009 |
nsubj |
most,are |
R1171 |
T3022 |
T3021 |
prep |
of,most |
R1172 |
T3023 |
T3024 |
det |
the,cells |
R1173 |
T3024 |
T3022 |
pobj |
cells,of |
R1174 |
T3025 |
T3024 |
amod |
immunoreactive,cells |
R1175 |
T3026 |
T3009 |
acomp |
present,are |
R1176 |
T3027 |
T3009 |
prep |
in,are |
R1177 |
T3028 |
T3029 |
det |
the,zone |
R1178 |
T3029 |
T3027 |
pobj |
zone,in |
R1179 |
T3030 |
T3029 |
amod |
ventricular,zone |
R1180 |
T3031 |
T3029 |
amod |
germinative,zone |
R1181 |
T3032 |
T3029 |
acl |
surrounding,zone |
R1182 |
T3033 |
T3034 |
det |
the,ventricle |
R1183 |
T3034 |
T3032 |
dobj |
ventricle,surrounding |
R1184 |
T3035 |
T3034 |
amod |
lateral,ventricle |
R1185 |
T3036 |
T3037 |
punct |
(,E13 |
R1186 |
T3037 |
T3009 |
parataxis |
E13,are |
R1187 |
T3038 |
T3039 |
dep |
Fig.,E16 |
R1188 |
T3039 |
T3037 |
dep |
E16,E13 |
R1189 |
T3040 |
T3038 |
nummod |
3A,Fig. |
R1190 |
T3041 |
T3039 |
punct |
", ",E16 |
R1191 |
T3042 |
T3039 |
compound |
overview,E16 |
R1192 |
T3043 |
T3037 |
punct |
;,E13 |
R1193 |
T3044 |
T3037 |
dep |
Fig.,E13 |
R1194 |
T3045 |
T3044 |
nummod |
3H,Fig. |
R1195 |
T3046 |
T3037 |
punct |
", ",E13 |
R1196 |
T3047 |
T3037 |
compound |
overview,E13 |
R1197 |
T3048 |
T3037 |
punct |
),E13 |
R1198 |
T3049 |
T3009 |
punct |
.,are |
R1199 |
T3051 |
T3052 |
det |
A,inspection |
R1200 |
T3052 |
T3054 |
nsubj |
inspection,revealed |
R1201 |
T3053 |
T3052 |
amod |
closer,inspection |
R1202 |
T3055 |
T3056 |
mark |
that,localized |
R1203 |
T3056 |
T3054 |
ccomp |
localized,revealed |
R1204 |
T3057 |
T3058 |
compound |
Annexin,A7 |
R1205 |
T3058 |
T3056 |
nsubjpass |
A7,localized |
R1206 |
T3059 |
T3056 |
auxpass |
is,localized |
R1207 |
T3060 |
T3056 |
advmod |
mainly,localized |
R1208 |
T3061 |
T3056 |
prep |
in,localized |
R1209 |
T3062 |
T3063 |
det |
the,cytosol |
R1210 |
T3063 |
T3061 |
pobj |
cytosol,in |
R1211 |
T3064 |
T3056 |
prep |
at,localized |
R1212 |
T3065 |
T3064 |
pobj |
E13,at |
R1213 |
T3066 |
T3067 |
punct |
(,E |
R1214 |
T3067 |
T3054 |
parataxis |
E,revealed |
R1215 |
T3068 |
T3067 |
nmod |
Fig.,E |
R1216 |
T3069 |
T3067 |
nummod |
3B,E |
R1217 |
T3070 |
T3067 |
punct |
",",E |
R1218 |
T3071 |
T3067 |
punct |
),E |
R1219 |
T3072 |
T3054 |
punct |
.,revealed |
R1220 |
T3074 |
T3075 |
prep |
During,rounded |
R1221 |
T3076 |
T3077 |
amod |
further,development |
R1222 |
T3077 |
T3074 |
pobj |
development,During |
R1223 |
T3078 |
T3079 |
det |
the,cells |
R1224 |
T3079 |
T3075 |
nsubj |
cells,rounded |
R1225 |
T3080 |
T3079 |
amod |
immature,cells |
R1226 |
T3081 |
T3075 |
aux |
have,rounded |
R1227 |
T3082 |
T3075 |
prt |
up,rounded |
R1228 |
T3083 |
T3084 |
nummod |
two,days |
R1229 |
T3084 |
T3085 |
npadvmod |
days,later |
R1230 |
T3085 |
T3075 |
advmod |
later,rounded |
R1231 |
T3086 |
T3075 |
punct |
", ",rounded |
R1232 |
T3087 |
T3075 |
cc |
but,rounded |
R1233 |
T3088 |
T3089 |
nsubj |
they,retain |
R1234 |
T3089 |
T3075 |
conj |
retain,rounded |
R1235 |
T3090 |
T3091 |
compound |
Annexin,A7 |
R1236 |
T3091 |
T3089 |
dobj |
A7,retain |
R1237 |
T3092 |
T3089 |
prep |
in,retain |
R1238 |
T3093 |
T3094 |
det |
the,cytosol |
R1239 |
T3094 |
T3092 |
pobj |
cytosol,in |
R1240 |
T3095 |
T3089 |
prep |
at,retain |
R1241 |
T3096 |
T3095 |
pobj |
E15,at |
R1242 |
T3097 |
T3098 |
punct |
(,F |
R1243 |
T3098 |
T3089 |
parataxis |
F,retain |
R1244 |
T3099 |
T3098 |
nmod |
Fig.,F |
R1245 |
T3100 |
T3098 |
nummod |
3C,F |
R1246 |
T3101 |
T3098 |
punct |
",",F |
R1247 |
T3102 |
T3098 |
punct |
),F |
R1248 |
T3103 |
T3075 |
punct |
.,rounded |
R1249 |
T3105 |
T3106 |
prep |
At,observed |
R1250 |
T3107 |
T3105 |
pobj |
E16,At |
R1251 |
T3108 |
T3106 |
nsubj |
we,observed |
R1252 |
T3109 |
T3110 |
det |
the,staining |
R1253 |
T3110 |
T3106 |
dobj |
staining,observed |
R1254 |
T3111 |
T3110 |
amod |
first,staining |
R1255 |
T3112 |
T3110 |
amod |
prominent,staining |
R1256 |
T3113 |
T3110 |
amod |
nuclear,staining |
R1257 |
T3114 |
T3110 |
prep |
of,staining |
R1258 |
T3115 |
T3116 |
compound |
Annexin,A7 |
R1259 |
T3116 |
T3114 |
pobj |
A7,of |
R1260 |
T3117 |
T3118 |
punct |
(,G |
R1261 |
T3118 |
T3110 |
parataxis |
G,staining |
R1262 |
T3119 |
T3118 |
nmod |
Fig.,G |
R1263 |
T3120 |
T3118 |
nmod |
3D,G |
R1264 |
T3121 |
T3118 |
punct |
",",G |
R1265 |
T3122 |
T3118 |
punct |
),G |
R1266 |
T3123 |
T3106 |
prep |
in,observed |
R1267 |
T3124 |
T3123 |
pobj |
cells,in |
R1268 |
T3125 |
T3124 |
prep |
of,cells |
R1269 |
T3126 |
T3127 |
det |
the,zone |
R1270 |
T3127 |
T3125 |
pobj |
zone,of |
R1271 |
T3128 |
T3127 |
amod |
intermediate,zone |
R1272 |
T3129 |
T3130 |
punct |
(,oval |
R1273 |
T3130 |
T3127 |
parataxis |
oval,zone |
R1274 |
T3131 |
T3132 |
compound |
Fig.,3I |
R1275 |
T3132 |
T3130 |
dep |
3I,oval |
R1276 |
T3133 |
T3130 |
punct |
", ",oval |
R1277 |
T3134 |
T3130 |
punct |
),oval |
R1278 |
T3135 |
T3127 |
punct |
", ",zone |
R1279 |
T3136 |
T3137 |
dep |
which,contains |
R1280 |
T3137 |
T3127 |
relcl |
contains,zone |
R1281 |
T3138 |
T3137 |
dobj |
neurons,contains |
R1282 |
T3139 |
T3140 |
advmod |
radially,migrating |
R1283 |
T3140 |
T3138 |
acl |
migrating,neurons |
R1284 |
T3141 |
T3140 |
prep |
towards,migrating |
R1285 |
T3142 |
T3143 |
det |
the,cortex |
R1286 |
T3143 |
T3141 |
pobj |
cortex,towards |
R1287 |
T3144 |
T3143 |
amod |
growing,cortex |
R1288 |
T3145 |
T3143 |
compound |
neopallium,cortex |
R1289 |
T3146 |
T3147 |
punct |
[,14 |
R1290 |
T3147 |
T3143 |
parataxis |
14,cortex |
R1291 |
T3148 |
T3147 |
nummod |
13,14 |
R1292 |
T3149 |
T3147 |
punct |
",",14 |
R1293 |
T3150 |
T3147 |
punct |
],14 |
R1294 |
T3151 |
T3138 |
cc |
and,neurons |
R1295 |
T3152 |
T3151 |
advmod |
also,and |
R1296 |
T3153 |
T3154 |
amod |
glial,cells |
R1297 |
T3154 |
T3138 |
conj |
cells,neurons |
R1298 |
T3155 |
T3154 |
acl |
forming,cells |
R1299 |
T3156 |
T3157 |
det |
the,matter |
R1300 |
T3157 |
T3155 |
dobj |
matter,forming |
R1301 |
T3158 |
T3157 |
amod |
white,matter |
R1302 |
T3159 |
T3157 |
prep |
of,matter |
R1303 |
T3160 |
T3161 |
det |
the,cortex |
R1304 |
T3161 |
T3159 |
pobj |
cortex,of |
R1305 |
T3162 |
T3161 |
amod |
adult,cortex |
R1306 |
T3163 |
T3106 |
punct |
.,observed |
R1307 |
T3165 |
T3166 |
nsubj |
Cells,exhibit |
R1308 |
T3167 |
T3165 |
acl |
located,Cells |
R1309 |
T3168 |
T3169 |
advmod |
marginally,in |
R1310 |
T3169 |
T3167 |
prep |
in,located |
R1311 |
T3170 |
T3171 |
det |
the,cortex |
R1312 |
T3171 |
T3169 |
pobj |
cortex,in |
R1313 |
T3172 |
T3171 |
amod |
neopallial,cortex |
R1314 |
T3173 |
T3166 |
advmod |
also,exhibit |
R1315 |
T3174 |
T3175 |
det |
a,stain |
R1316 |
T3175 |
T3166 |
dobj |
stain,exhibit |
R1317 |
T3176 |
T3175 |
amod |
nuclear,stain |
R1318 |
T3177 |
T3166 |
punct |
.,exhibit |
R1319 |
T3179 |
T3180 |
nsubj |
Labelling,is |
R1320 |
T3181 |
T3179 |
prep |
in,Labelling |
R1321 |
T3182 |
T3183 |
det |
the,zone |
R1322 |
T3183 |
T3181 |
pobj |
zone,in |
R1323 |
T3184 |
T3183 |
amod |
ventricular,zone |
R1324 |
T3185 |
T3183 |
amod |
germinative,zone |
R1325 |
T3186 |
T3187 |
advmod |
less,prominent |
R1326 |
T3187 |
T3180 |
acomp |
prominent,is |
R1327 |
T3188 |
T3180 |
cc |
and,is |
R1328 |
T3189 |
T3190 |
det |
the,cytosol |
R1329 |
T3190 |
T3191 |
nsubj |
cytosol,is |
R1330 |
T3191 |
T3180 |
conj |
is,is |
R1331 |
T3192 |
T3193 |
neg |
no,longer |
R1332 |
T3193 |
T3191 |
advmod |
longer,is |
R1333 |
T3194 |
T3195 |
advmod |
more,strongly |
R1334 |
T3195 |
T3196 |
advmod |
strongly,stained |
R1335 |
T3196 |
T3191 |
acomp |
stained,is |
R1336 |
T3197 |
T3196 |
prep |
than,stained |
R1337 |
T3198 |
T3199 |
det |
the,nucleus |
R1338 |
T3199 |
T3197 |
pobj |
nucleus,than |
R1339 |
T3200 |
T3191 |
punct |
.,is |
R1340 |
T3569 |
T3570 |
amod |
Mature,neurons |
R1341 |
T3570 |
T3571 |
nsubj |
neurons,show |
R1342 |
T3572 |
T3570 |
prep |
in,neurons |
R1343 |
T3573 |
T3574 |
amod |
adult,brain |
R1344 |
T3574 |
T3572 |
pobj |
brain,in |
R1345 |
T3575 |
T3574 |
compound |
mouse,brain |
R1346 |
T3576 |
T3577 |
det |
an,staining |
R1347 |
T3577 |
T3571 |
dobj |
staining,show |
R1348 |
T3578 |
T3577 |
amod |
intense,staining |
R1349 |
T3579 |
T3577 |
amod |
nuclear,staining |
R1350 |
T3580 |
T3577 |
prep |
of,staining |
R1351 |
T3581 |
T3582 |
compound |
Annexin,A7 |
R1352 |
T3582 |
T3580 |
pobj |
A7,of |
R1353 |
T3584 |
T3585 |
preconj |
Both,isoforms |
R1354 |
T3585 |
T3588 |
nsubjpass |
isoforms,found |
R1355 |
T3586 |
T3587 |
compound |
Annexin,A7 |
R1356 |
T3587 |
T3585 |
compound |
A7,isoforms |
R1357 |
T3589 |
T3588 |
auxpass |
are,found |
R1358 |
T3590 |
T3588 |
prep |
in,found |
R1359 |
T3591 |
T3592 |
amod |
adult,tissue |
R1360 |
T3592 |
T3590 |
pobj |
tissue,in |
R1361 |
T3593 |
T3592 |
compound |
brain,tissue |
R1362 |
T3594 |
T3595 |
punct |
(,1B |
R1363 |
T3595 |
T3588 |
parataxis |
1B,found |
R1364 |
T3596 |
T3595 |
compound |
Fig.,1B |
R1365 |
T3597 |
T3595 |
punct |
),1B |
R1366 |
T3598 |
T3588 |
punct |
.,found |
R1367 |
T3600 |
T3601 |
prep |
In,observed |
R1368 |
T3602 |
T3600 |
amod |
general,In |
R1369 |
T3603 |
T3601 |
punct |
", ",observed |
R1370 |
T3604 |
T3601 |
nsubj |
we,observed |
R1371 |
T3605 |
T3606 |
nummod |
two,patterns |
R1372 |
T3606 |
T3601 |
dobj |
patterns,observed |
R1373 |
T3607 |
T3606 |
amod |
characteristic,patterns |
R1374 |
T3608 |
T3606 |
compound |
staining,patterns |
R1375 |
T3609 |
T3606 |
prep |
for,patterns |
R1376 |
T3610 |
T3611 |
compound |
Annexin,A7 |
R1377 |
T3611 |
T3609 |
pobj |
A7,for |
R1378 |
T3612 |
T3606 |
punct |
", ",patterns |
R1379 |
T3613 |
T3614 |
det |
a,stain |
R1380 |
T3614 |
T3606 |
appos |
stain,patterns |
R1381 |
T3615 |
T3614 |
amod |
prominent,stain |
R1382 |
T3616 |
T3614 |
amod |
nuclear,stain |
R1383 |
T3617 |
T3614 |
prep |
in,stain |
R1384 |
T3618 |
T3617 |
pobj |
neurons,in |
R1385 |
T3619 |
T3614 |
punct |
(,stain |
R1386 |
T3620 |
T3614 |
acl |
determined,stain |
R1387 |
T3621 |
T3620 |
prep |
by,determined |
R1388 |
T3622 |
T3621 |
pobj |
lack,by |
R1389 |
T3623 |
T3622 |
prep |
of,lack |
R1390 |
T3624 |
T3625 |
nmod |
GFAP,morphology |
R1391 |
T3625 |
T3623 |
pobj |
morphology,of |
R1392 |
T3626 |
T3625 |
punct |
-,morphology |
R1393 |
T3627 |
T3625 |
nmod |
immunoreactivity,morphology |
R1394 |
T3628 |
T3625 |
punct |
", ",morphology |
R1395 |
T3629 |
T3625 |
nmod |
localization,morphology |
R1396 |
T3630 |
T3625 |
punct |
", ",morphology |
R1397 |
T3631 |
T3614 |
punct |
),stain |
R1398 |
T3632 |
T3614 |
cc |
and,stain |
R1399 |
T3633 |
T3634 |
det |
a,stain |
R1400 |
T3634 |
T3614 |
conj |
stain,stain |
R1401 |
T3635 |
T3634 |
amod |
cytoplasmic,stain |
R1402 |
T3636 |
T3635 |
cc |
and,cytoplasmic |
R1403 |
T3637 |
T3635 |
conj |
nuclear,cytoplasmic |
R1404 |
T3638 |
T3634 |
prep |
in,stain |
R1405 |
T3639 |
T3638 |
pobj |
astrocytes,in |
R1406 |
T3640 |
T3641 |
punct |
(,positive |
R1407 |
T3641 |
T3639 |
parataxis |
positive,astrocytes |
R1408 |
T3642 |
T3641 |
npadvmod |
GFAP,positive |
R1409 |
T3643 |
T3641 |
punct |
-,positive |
R1410 |
T3644 |
T3641 |
punct |
),positive |
R1411 |
T3645 |
T3601 |
prep |
in,observed |
R1412 |
T3646 |
T3647 |
det |
all,areas |
R1413 |
T3647 |
T3645 |
pobj |
areas,in |
R1414 |
T3648 |
T3647 |
prep |
of,areas |
R1415 |
T3649 |
T3650 |
det |
the,brain |
R1416 |
T3650 |
T3648 |
pobj |
brain,of |
R1417 |
T3651 |
T3650 |
amod |
mature,brain |
R1418 |
T3652 |
T3650 |
amod |
murine,brain |
R1419 |
T3653 |
T3601 |
punct |
.,observed |
R1420 |
T3655 |
T3656 |
prep |
In,enriched |
R1421 |
T3657 |
T3658 |
det |
the,neocortex |
R1422 |
T3658 |
T3655 |
pobj |
neocortex,In |
R1423 |
T3659 |
T3660 |
punct |
(,isocortex |
R1424 |
T3660 |
T3658 |
parataxis |
isocortex,neocortex |
R1425 |
T3661 |
T3660 |
punct |
),isocortex |
R1426 |
T3662 |
T3663 |
compound |
Annexin,A7 |
R1427 |
T3663 |
T3656 |
nsubjpass |
A7,enriched |
R1428 |
T3664 |
T3656 |
auxpass |
was,enriched |
R1429 |
T3665 |
T3656 |
advmod |
strongly,enriched |
R1430 |
T3666 |
T3656 |
prep |
in,enriched |
R1431 |
T3667 |
T3666 |
pobj |
nuclei,in |
R1432 |
T3668 |
T3667 |
prep |
of,nuclei |
R1433 |
T3669 |
T3668 |
pobj |
neurons,of |
R1434 |
T3670 |
T3669 |
prep |
of,neurons |
R1435 |
T3671 |
T3672 |
det |
all,laminae |
R1436 |
T3672 |
T3670 |
pobj |
laminae,of |
R1437 |
T3673 |
T3672 |
nummod |
six,laminae |
R1438 |
T3674 |
T3672 |
amod |
cortical,laminae |
R1439 |
T3675 |
T3676 |
punct |
(,section |
R1440 |
T3676 |
T3656 |
parataxis |
section,enriched |
R1441 |
T3677 |
T3678 |
compound |
Fig.,4A |
R1442 |
T3678 |
T3676 |
dep |
4A,section |
R1443 |
T3679 |
T3676 |
punct |
", ",section |
R1444 |
T3680 |
T3676 |
acl |
derived,section |
R1445 |
T3681 |
T3680 |
prep |
from,derived |
R1446 |
T3682 |
T3683 |
compound |
cortex,temporalis |
R1447 |
T3683 |
T3681 |
pobj |
temporalis,from |
R1448 |
T3684 |
T3676 |
punct |
),section |
R1449 |
T3685 |
T3656 |
punct |
.,enriched |
R1450 |
T3687 |
T3688 |
prep |
For,characterized |
R1451 |
T3689 |
T3687 |
pobj |
control,For |
R1452 |
T3690 |
T3688 |
punct |
", ",characterized |
R1453 |
T3691 |
T3688 |
prep |
in,characterized |
R1454 |
T3692 |
T3693 |
det |
the,brain |
R1455 |
T3693 |
T3691 |
pobj |
brain,in |
R1456 |
T3694 |
T3693 |
amod |
adult,brain |
R1457 |
T3695 |
T3696 |
nmod |
type,astrocytes |
R1458 |
T3696 |
T3688 |
nsubjpass |
astrocytes,characterized |
R1459 |
T3697 |
T3695 |
punct |
-,type |
R1460 |
T3698 |
T3695 |
nummod |
1,type |
R1461 |
T3699 |
T3688 |
aux |
can,characterized |
R1462 |
T3700 |
T3688 |
auxpass |
be,characterized |
R1463 |
T3701 |
T3688 |
agent |
by,characterized |
R1464 |
T3702 |
T3703 |
det |
a,stain |
R1465 |
T3703 |
T3701 |
pobj |
stain,by |
R1466 |
T3704 |
T3703 |
amod |
positive,stain |
R1467 |
T3705 |
T3703 |
compound |
GFAP,stain |
R1468 |
T3706 |
T3703 |
punct |
-,stain |
R1469 |
T3707 |
T3708 |
punct |
(,4B |
R1470 |
T3708 |
T3688 |
parataxis |
4B,characterized |
R1471 |
T3709 |
T3708 |
compound |
Fig.,4B |
R1472 |
T3710 |
T3708 |
punct |
),4B |
R1473 |
T3711 |
T3688 |
punct |
.,characterized |
R1474 |
T3713 |
T3714 |
compound |
Fig.,4F |
R1475 |
T3714 |
T3715 |
nsubj |
4F,demonstrates |
R1476 |
T3716 |
T3717 |
det |
the,presence |
R1477 |
T3717 |
T3715 |
dobj |
presence,demonstrates |
R1478 |
T3718 |
T3717 |
prep |
of,presence |
R1479 |
T3719 |
T3720 |
amod |
nuclear,A7 |
R1480 |
T3720 |
T3718 |
pobj |
A7,of |
R1481 |
T3721 |
T3720 |
compound |
Annexin,A7 |
R1482 |
T3722 |
T3715 |
prep |
in,demonstrates |
R1483 |
T3723 |
T3724 |
det |
a,neuron |
R1484 |
T3724 |
T3722 |
pobj |
neuron,in |
R1485 |
T3725 |
T3724 |
amod |
neocortical,neuron |
R1486 |
T3726 |
T3724 |
prep |
of,neuron |
R1487 |
T3727 |
T3728 |
det |
the,layer |
R1488 |
T3728 |
T3726 |
pobj |
layer,of |
R1489 |
T3729 |
T3728 |
amod |
external,layer |
R1490 |
T3730 |
T3728 |
amod |
granular,layer |
R1491 |
T3731 |
T3732 |
punct |
(,layer |
R1492 |
T3732 |
T3728 |
parataxis |
layer,layer |
R1493 |
T3733 |
T3732 |
nummod |
II,layer |
R1494 |
T3734 |
T3732 |
punct |
),layer |
R1495 |
T3735 |
T3722 |
cc |
and,in |
R1496 |
T3736 |
T3722 |
conj |
in,in |
R1497 |
T3737 |
T3738 |
det |
an,cell |
R1498 |
T3738 |
T3736 |
pobj |
cell,in |
R1499 |
T3739 |
T3738 |
amod |
astrocytic,cell |
R1500 |
T3740 |
T3738 |
prep |
of,cell |
R1501 |
T3741 |
T3742 |
det |
the,layer |
R1502 |
T3742 |
T3740 |
pobj |
layer,of |
R1503 |
T3743 |
T3742 |
amod |
neocortical,layer |
R1504 |
T3744 |
T3742 |
amod |
molecular,layer |
R1505 |
T3745 |
T3746 |
punct |
(,layer |
R1506 |
T3746 |
T3742 |
parataxis |
layer,layer |
R1507 |
T3747 |
T3746 |
nummod |
I,layer |
R1508 |
T3748 |
T3746 |
punct |
),layer |
R1529 |
T3771 |
T3769 |
pobj |
isocortex,of |
R1530 |
T3772 |
T3773 |
det |
a,immunoreactivity |
R1531 |
T3773 |
T3768 |
dobj |
immunoreactivity,showed |
R1532 |
T3774 |
T3773 |
amod |
strong,immunoreactivity |
R1533 |
T3775 |
T3768 |
prep |
in,showed |
R1534 |
T3776 |
T3777 |
preconj |
both,nucleus |
R1535 |
T3777 |
T3775 |
pobj |
nucleus,in |
R1536 |
T3778 |
T3777 |
punct |
", ",nucleus |
R1537 |
T3779 |
T3777 |
cc |
and,nucleus |
R1538 |
T3780 |
T3777 |
conj |
cytosol,nucleus |
R1539 |
T3781 |
T3768 |
punct |
", ",showed |
R1540 |
T3782 |
T3768 |
cc |
and,showed |
R1541 |
T3783 |
T3784 |
auxpass |
was,identified |
R1542 |
T3784 |
T3768 |
conj |
identified,showed |
R1543 |
T3785 |
T3784 |
prep |
as,identified |
R1544 |
T3786 |
T3787 |
amod |
pyramidal,neuron |
R1545 |
T3787 |
T3785 |
pobj |
neuron,as |
R1546 |
T3788 |
T3784 |
prep |
based,identified |
R1547 |
T3789 |
T3788 |
prep |
on,based |
R1548 |
T3790 |
T3789 |
pobj |
morphology,on |
R1549 |
T3791 |
T3790 |
cc |
and,morphology |
R1550 |
T3792 |
T3790 |
conj |
absence,morphology |
R1551 |
T3793 |
T3792 |
prep |
of,absence |
R1552 |
T3794 |
T3795 |
compound |
GFAP,immunoreactivity |
R1553 |
T3795 |
T3793 |
pobj |
immunoreactivity,of |
R1554 |
T3796 |
T3768 |
punct |
.,showed |
R1555 |
T3798 |
T3799 |
advmod |
However,is |
R1556 |
T3800 |
T3799 |
punct |
", ",is |
R1557 |
T3801 |
T3802 |
compound |
Annexin,A7 |
R1558 |
T3802 |
T3799 |
nsubj |
A7,is |
R1559 |
T3803 |
T3799 |
advmod |
apparently,is |
R1560 |
T3804 |
T3805 |
advmod |
more,enriched |
R1561 |
T3805 |
T3799 |
acomp |
enriched,is |
R1562 |
T3806 |
T3799 |
prep |
in,is |
R1563 |
T3807 |
T3808 |
det |
the,nucleus |
R1564 |
T3808 |
T3806 |
pobj |
nucleus,in |
R1565 |
T3809 |
T3810 |
punct |
(,4E |
R1566 |
T3810 |
T3799 |
parataxis |
4E,is |
R1567 |
T3811 |
T3810 |
compound |
Fig.,4E |
R1568 |
T3812 |
T3810 |
punct |
),4E |
R1569 |
T3813 |
T3799 |
punct |
.,is |
R1574 |
T3821 |
T3817 |
nsubj |
we,detected |
R1575 |
T3822 |
T3823 |
amod |
prominent,immunostaining |
R1576 |
T3823 |
T3817 |
dobj |
immunostaining,detected |
R1577 |
T3824 |
T3825 |
compound |
Annexin,A7 |
R1578 |
T3825 |
T3823 |
compound |
A7,immunostaining |
R1579 |
T3826 |
T3823 |
prep |
in,immunostaining |
R1580 |
T3827 |
T3828 |
det |
the,pyramidalis |
R1581 |
T3828 |
T3826 |
pobj |
pyramidalis,in |
R1582 |
T3829 |
T3828 |
compound |
stratum,pyramidalis |
R1583 |
T3830 |
T3826 |
cc |
and,in |
R1584 |
T3831 |
T3826 |
conj |
in,in |
R1585 |
T3832 |
T3833 |
det |
the,gyrus |
R1586 |
T3833 |
T3831 |
pobj |
gyrus,in |
R1587 |
T3834 |
T3833 |
compound |
dentate,gyrus |
R1588 |
T3835 |
T3817 |
cc |
and,detected |
R1589 |
T3836 |
T3835 |
advmod |
also,and |
R1590 |
T3837 |
T3838 |
det |
a,staining |
R1591 |
T3838 |
T3817 |
conj |
staining,detected |
R1592 |
T3839 |
T3838 |
amod |
weak,staining |
R1593 |
T3840 |
T3838 |
amod |
astrocytic,staining |
R1594 |
T3841 |
T3842 |
punct |
(,4C |
R1595 |
T3842 |
T3838 |
parataxis |
4C,staining |
R1596 |
T3843 |
T3842 |
compound |
Fig.,4C |
R1597 |
T3844 |
T3842 |
punct |
),4C |
R1598 |
T3845 |
T3817 |
punct |
.,detected |
R1599 |
T3847 |
T3848 |
det |
The,astrocytes |
R1600 |
T3848 |
T3849 |
nsubjpass |
astrocytes,localized |
R1601 |
T3850 |
T3849 |
auxpass |
are,localized |
R1602 |
T3851 |
T3849 |
advmod |
mainly,localized |
R1603 |
T3852 |
T3849 |
prep |
in,localized |
R1604 |
T3853 |
T3854 |
det |
the,area |
R1605 |
T3854 |
T3852 |
pobj |
area,in |
R1606 |
T3855 |
T3854 |
prep |
between,area |
R1607 |
T3856 |
T3855 |
pobj |
cells,between |
R1608 |
T3857 |
T3856 |
acl |
exhibiting,cells |
R1609 |
T3858 |
T3859 |
amod |
nuclear,staining |
R1610 |
T3859 |
T3857 |
dobj |
staining,exhibiting |
R1611 |
T3860 |
T3861 |
compound |
Annexin,A7 |
R1612 |
T3861 |
T3859 |
compound |
A7,staining |
R1613 |
T3862 |
T3849 |
cc |
and,localized |
R1614 |
T3863 |
T3849 |
conj |
show,localized |
R1615 |
T3864 |
T3865 |
det |
the,protein |
R1616 |
T3865 |
T3863 |
dobj |
protein,show |
R1617 |
T3866 |
T3863 |
advmod |
also,show |
R1618 |
T3867 |
T3863 |
prep |
in,show |
R1619 |
T3868 |
T3869 |
det |
the,nucleus |
R1620 |
T3869 |
T3867 |
pobj |
nucleus,in |
R1621 |
T3870 |
T3869 |
cc |
and,nucleus |
R1622 |
T3871 |
T3872 |
det |
the,cytoplasm |
R1623 |
T3872 |
T3869 |
conj |
cytoplasm,nucleus |
R1624 |
T3873 |
T3874 |
punct |
(,shown |
R1625 |
T3874 |
T3863 |
parataxis |
shown,show |
R1626 |
T3875 |
T3874 |
nsubj |
data,shown |
R1627 |
T3876 |
T3874 |
neg |
not,shown |
R1628 |
T3877 |
T3874 |
punct |
),shown |
R1629 |
T3878 |
T3849 |
punct |
.,localized |
R1630 |
T3880 |
T3881 |
advmod |
When,analyzed |
R1631 |
T3881 |
T3883 |
advcl |
analyzed,found |
R1632 |
T3882 |
T3881 |
nsubj |
we,analyzed |
R1633 |
T3884 |
T3885 |
det |
the,neurons |
R1634 |
T3885 |
T3881 |
dobj |
neurons,analyzed |
R1635 |
T3886 |
T3885 |
amod |
pyramidal,neurons |
R1636 |
T3887 |
T3881 |
prep |
in,analyzed |
R1637 |
T3888 |
T3889 |
det |
the,hippocampus |
R1638 |
T3889 |
T3887 |
pobj |
hippocampus,in |
R1639 |
T3890 |
T3881 |
prep |
at,analyzed |
R1640 |
T3891 |
T3892 |
det |
a,magnification |
R1641 |
T3892 |
T3890 |
pobj |
magnification,at |
R1642 |
T3893 |
T3892 |
amod |
higher,magnification |
R1643 |
T3894 |
T3883 |
nsubj |
we,found |
R1644 |
T3895 |
T3896 |
mark |
that,localized |
R1645 |
T3896 |
T3883 |
ccomp |
localized,found |
R1646 |
T3897 |
T3898 |
compound |
Annexin,A7 |
R1647 |
T3898 |
T3896 |
nsubj |
A7,localized |
R1648 |
T3899 |
T3896 |
advmod |
again,localized |
R1649 |
T3900 |
T3896 |
advmod |
mainly,localized |
R1650 |
T3901 |
T3896 |
prep |
to,localized |
R1651 |
T3902 |
T3903 |
det |
the,nuclei |
R1652 |
T3903 |
T3901 |
pobj |
nuclei,to |
R1653 |
T3904 |
T3905 |
punct |
(,4G |
R1654 |
T3905 |
T3883 |
parataxis |
4G,found |
R1655 |
T3906 |
T3905 |
compound |
Fig.,4G |
R1656 |
T3907 |
T3905 |
punct |
),4G |
R1657 |
T3908 |
T3883 |
punct |
.,found |
R1658 |
T3910 |
T3911 |
amod |
Polyclonal,antibodies |
R1659 |
T3911 |
T3912 |
nsubj |
antibodies,gave |
R1660 |
T3913 |
T3914 |
det |
a,pattern |
R1661 |
T3914 |
T3912 |
dobj |
pattern,gave |
R1662 |
T3915 |
T3914 |
amod |
similar,pattern |
R1663 |
T3916 |
T3914 |
compound |
staining,pattern |
R1664 |
T3917 |
T3918 |
punct |
(,shown |
R1665 |
T3918 |
T3912 |
parataxis |
shown,gave |
R1666 |
T3919 |
T3918 |
nsubj |
data,shown |
R1667 |
T3920 |
T3918 |
neg |
not,shown |
R1668 |
T3921 |
T3918 |
punct |
),shown |
R1669 |
T3922 |
T3912 |
punct |
.,gave |
R1670 |
T3924 |
T3925 |
det |
The,staining |
R1671 |
T3925 |
T3928 |
nsubj |
staining,was |
R1672 |
T3926 |
T3925 |
amod |
intense,staining |
R1673 |
T3927 |
T3925 |
amod |
nuclear,staining |
R1674 |
T3929 |
T3928 |
acomp |
absent,was |
R1675 |
T3930 |
T3928 |
prep |
in,was |
R1676 |
T3931 |
T3930 |
pobj |
controls,in |
R1677 |
T3932 |
T3933 |
preconj |
either,stained |
R1678 |
T3933 |
T3931 |
acl |
stained,controls |
R1679 |
T3934 |
T3935 |
advmod |
only,with |
R1680 |
T3935 |
T3933 |
prep |
with,stained |
R1681 |
T3936 |
T3937 |
det |
the,antibody |
R1682 |
T3937 |
T3935 |
pobj |
antibody,with |
R1683 |
T3938 |
T3937 |
amod |
secondary,antibody |
R1684 |
T3939 |
T3940 |
punct |
(,4D |
R1685 |
T3940 |
T3933 |
parataxis |
4D,stained |
R1686 |
T3941 |
T3940 |
compound |
Fig.,4D |
R1687 |
T3942 |
T3940 |
punct |
),4D |
R1688 |
T3943 |
T3930 |
cc |
or,in |
R1689 |
T3944 |
T3930 |
conj |
in,in |
R1690 |
T3945 |
T3946 |
compound |
brain,sections |
R1691 |
T3946 |
T3944 |
pobj |
sections,in |
R1692 |
T3947 |
T3946 |
acl |
derived,sections |
R1693 |
T3948 |
T3947 |
prep |
from,derived |
R1694 |
T3949 |
T3950 |
nmod |
AnxA7,mice |
R1695 |
T3950 |
T3948 |
pobj |
mice,from |
R1696 |
T3951 |
T3949 |
punct |
-,AnxA7 |
R1697 |
T3952 |
T3949 |
punct |
/,AnxA7 |
R1698 |
T3953 |
T3949 |
punct |
-,AnxA7 |
R1699 |
T3954 |
T3950 |
acl |
stained,mice |
R1700 |
T3955 |
T3954 |
prep |
for,stained |
R1701 |
T3956 |
T3957 |
compound |
Annexin,A7 |
R1702 |
T3957 |
T3955 |
pobj |
A7,for |
R1703 |
T3958 |
T3959 |
punct |
(,4H |
R1704 |
T3959 |
T3946 |
parataxis |
4H,sections |
R1705 |
T3960 |
T3959 |
compound |
Fig.,4H |
R1706 |
T3961 |
T3959 |
punct |
),4H |
R1707 |
T3962 |
T3928 |
punct |
.,was |
R1708 |
T3964 |
T3965 |
det |
The,staining |
R1709 |
T3965 |
T3967 |
nsubj |
staining,is |
R1710 |
T3966 |
T3965 |
amod |
residual,staining |
R1711 |
T3968 |
T3965 |
acl |
seen,staining |
R1712 |
T3969 |
T3968 |
prep |
in,seen |
R1713 |
T3970 |
T3971 |
det |
the,brain |
R1714 |
T3971 |
T3969 |
pobj |
brain,in |
R1715 |
T3972 |
T3971 |
nmod |
AnxA7,brain |
R1716 |
T3973 |
T3972 |
punct |
-,AnxA7 |
R1717 |
T3974 |
T3972 |
punct |
/,AnxA7 |
R1718 |
T3975 |
T3972 |
punct |
-,AnxA7 |
R1719 |
T3976 |
T3967 |
acomp |
unspecific,is |
R1720 |
T3977 |
T3978 |
mark |
as,is |
R1721 |
T3978 |
T3967 |
advcl |
is,is |
R1722 |
T3979 |
T3978 |
nsubj |
it,is |
R1723 |
T3980 |
T3978 |
advmod |
also,is |
R1724 |
T3981 |
T3978 |
acomp |
present,is |
R1725 |
T3982 |
T3978 |
prep |
in,is |
R1726 |
T3983 |
T3984 |
amod |
corresponding,controls |
R1727 |
T3984 |
T3982 |
pobj |
controls,in |
R1728 |
T3985 |
T3984 |
amod |
negative,controls |
R1729 |
T3986 |
T3984 |
acl |
lacking,controls |
R1730 |
T3987 |
T3988 |
det |
the,antibody |
R1731 |
T3988 |
T3986 |
dobj |
antibody,lacking |
R1732 |
T3989 |
T3988 |
amod |
primary,antibody |
R1733 |
T3990 |
T3967 |
punct |
.,is |
R1734 |
T3992 |
T3993 |
det |
The,absence |
R1735 |
T3993 |
T3994 |
nsubjpass |
absence,verified |
R1736 |
T3995 |
T3993 |
prep |
of,absence |
R1737 |
T3996 |
T3997 |
compound |
Annexin,A7 |
R1738 |
T3997 |
T3998 |
compound |
A7,protein |
R1739 |
T3998 |
T3995 |
pobj |
protein,of |
R1740 |
T3999 |
T3993 |
prep |
in,absence |
R1741 |
T4000 |
T3999 |
pobj |
brain,in |
R1742 |
T4001 |
T4000 |
cc |
and,brain |
R1743 |
T4002 |
T4003 |
amod |
other,tissues |
R1744 |
T4003 |
T4000 |
conj |
tissues,brain |
R1745 |
T4004 |
T4000 |
prep |
of,brain |
R1746 |
T4005 |
T4006 |
det |
the,mouse |
R1747 |
T4006 |
T4004 |
pobj |
mouse,of |
R1748 |
T4007 |
T4006 |
nmod |
AnxA7,mouse |
R1749 |
T4008 |
T4007 |
punct |
-,AnxA7 |
R1750 |
T4009 |
T4007 |
punct |
/,AnxA7 |
R1751 |
T4010 |
T4007 |
punct |
-,AnxA7 |
R1752 |
T4011 |
T3994 |
aux |
has,verified |
R1753 |
T4012 |
T3994 |
auxpass |
been,verified |
R1754 |
T4013 |
T3994 |
prep |
in,verified |
R1755 |
T4014 |
T4013 |
punct |
[,in |
R1756 |
T4015 |
T4013 |
pobj |
10,in |
R1757 |
T4016 |
T3994 |
punct |
],verified |
R1758 |
T4017 |
T3994 |
punct |
.,verified |
R1759 |
T4019 |
T4020 |
prep |
In,detected |
R1760 |
T4021 |
T4022 |
det |
another,study |
R1761 |
T4022 |
T4019 |
pobj |
study,In |
R1762 |
T4023 |
T4022 |
amod |
actual,study |
R1763 |
T4024 |
T4022 |
prep |
of,study |
R1764 |
T4025 |
T4026 |
det |
the,mouse |
R1765 |
T4026 |
T4024 |
pobj |
mouse,of |
R1766 |
T4027 |
T4026 |
amod |
knock,mouse |
R1767 |
T4028 |
T4027 |
prt |
out,knock |
R1768 |
T4029 |
T4020 |
nsubj |
we,detected |
R1769 |
T4030 |
T4031 |
det |
a,membrane |
R1770 |
T4031 |
T4020 |
dobj |
membrane,detected |
R1771 |
T4032 |
T4031 |
amod |
thickened,membrane |
R1772 |
T4033 |
T4031 |
amod |
basal,membrane |
R1773 |
T4034 |
T4031 |
cc |
and,membrane |
R1774 |
T4035 |
T4036 |
det |
a,space |
R1775 |
T4036 |
T4031 |
conj |
space,membrane |
R1776 |
T4037 |
T4036 |
amod |
widened,space |
R1777 |
T4038 |
T4036 |
amod |
intercellular,space |
R1778 |
T4039 |
T4020 |
punct |
.,detected |
R1779 |
T4041 |
T4042 |
nsubj |
This,cause |
R1780 |
T4043 |
T4042 |
aux |
may,cause |
R1781 |
T4044 |
T4045 |
amod |
unspecific,binding |
R1782 |
T4045 |
T4042 |
dobj |
binding,cause |
R1783 |
T4046 |
T4045 |
prep |
of,binding |
R1784 |
T4047 |
T4048 |
det |
the,antibody |
R1785 |
T4048 |
T4046 |
pobj |
antibody,of |
R1786 |
T4049 |
T4048 |
amod |
secondary,antibody |
R1787 |
T4050 |
T4042 |
punct |
.,cause |
R1788 |
T4052 |
T4053 |
det |
An,staining |
R1789 |
T4053 |
T4055 |
nsubjpass |
staining,found |
R1790 |
T4054 |
T4053 |
amod |
equal,staining |
R1791 |
T4056 |
T4053 |
prep |
of,staining |
R1792 |
T4057 |
T4058 |
compound |
Annexin,A7 |
R1793 |
T4058 |
T4056 |
pobj |
A7,of |
R1794 |
T4059 |
T4055 |
auxpass |
was,found |
R1795 |
T4060 |
T4055 |
advmod |
also,found |
R1796 |
T4061 |
T4055 |
prep |
in,found |
R1797 |
T4062 |
T4063 |
det |
the,cerebellum |
R1798 |
T4063 |
T4061 |
pobj |
cerebellum,in |
R1799 |
T4064 |
T4065 |
punct |
(,5A |
R1800 |
T4065 |
T4055 |
parataxis |
5A,found |
R1801 |
T4066 |
T4065 |
compound |
Fig.,5A |
R1802 |
T4067 |
T4065 |
punct |
),5A |
R1803 |
T4068 |
T4055 |
punct |
.,found |
R1804 |
T4070 |
T4071 |
nsubj |
Nuclei,show |
R1805 |
T4072 |
T4070 |
prep |
of,Nuclei |
R1806 |
T4073 |
T4072 |
pobj |
neurons,of |
R1807 |
T4074 |
T4073 |
prep |
in,neurons |
R1808 |
T4075 |
T4076 |
det |
the,granulosum |
R1809 |
T4076 |
T4074 |
pobj |
granulosum,in |
R1810 |
T4077 |
T4076 |
compound |
stratum,granulosum |
R1811 |
T4078 |
T4079 |
det |
the,staining |
R1812 |
T4079 |
T4071 |
dobj |
staining,show |
R1813 |
T4080 |
T4081 |
advmod |
most,prominent |
R1814 |
T4081 |
T4079 |
amod |
prominent,staining |
R1815 |
T4082 |
T4083 |
punct |
(,D |
R1816 |
T4083 |
T4071 |
parataxis |
D,show |
R1817 |
T4084 |
T4083 |
nmod |
Fig.,D |
R1818 |
T4085 |
T4083 |
nmod |
5C,D |
R1819 |
T4086 |
T4083 |
punct |
",",D |
R1820 |
T4087 |
T4083 |
punct |
),D |
R1821 |
T4088 |
T4071 |
punct |
.,show |
R1822 |
T4090 |
T4091 |
prep |
In,appear |
R1823 |
T4092 |
T4093 |
det |
the,moleculare |
R1824 |
T4093 |
T4090 |
pobj |
moleculare,In |
R1825 |
T4094 |
T4093 |
compound |
stratum,moleculare |
R1826 |
T4095 |
T4093 |
punct |
", ",moleculare |
R1827 |
T4096 |
T4097 |
dep |
which,is |
R1828 |
T4097 |
T4093 |
relcl |
is,moleculare |
R1829 |
T4098 |
T4097 |
acomp |
poor,is |
R1830 |
T4099 |
T4097 |
prep |
in,is |
R1831 |
T4100 |
T4101 |
compound |
cell,bodies |
R1832 |
T4101 |
T4099 |
pobj |
bodies,in |
R1833 |
T4102 |
T4101 |
prep |
of,bodies |
R1834 |
T4103 |
T4102 |
pobj |
neurons,of |
R1835 |
T4104 |
T4091 |
punct |
", ",appear |
R1836 |
T4105 |
T4106 |
advmod |
only,dots |
R1837 |
T4106 |
T4091 |
nsubj |
dots,appear |
R1838 |
T4107 |
T4106 |
det |
a,dots |
R1839 |
T4108 |
T4106 |
amod |
few,dots |
R1840 |
T4109 |
T4110 |
dep |
which,correspond |
R1841 |
T4110 |
T4091 |
ccomp |
correspond,appear |
R1842 |
T4111 |
T4110 |
advmod |
also,correspond |
R1843 |
T4112 |
T4110 |
prep |
to,correspond |
R1844 |
T4113 |
T4112 |
pobj |
nuclei,to |
R1845 |
T4114 |
T4113 |
prep |
of,nuclei |
R1846 |
T4115 |
T4114 |
pobj |
neurons,of |
R1847 |
T4116 |
T4091 |
punct |
.,appear |
R1848 |
T4118 |
T4119 |
nsubj |
Astrocytes,are |
R1849 |
T4120 |
T4119 |
advmod |
also,are |
R1850 |
T4121 |
T4119 |
acomp |
positive,are |
R1851 |
T4122 |
T4121 |
prep |
for,positive |
R1852 |
T4123 |
T4124 |
compound |
Annexin,A7 |
R1853 |
T4124 |
T4122 |
pobj |
A7,for |
R1854 |
T4125 |
T4119 |
prep |
in,are |
R1855 |
T4126 |
T4127 |
det |
the,nuclei |
R1856 |
T4127 |
T4125 |
pobj |
nuclei,in |
R1857 |
T4128 |
T4127 |
cc |
and,nuclei |
R1858 |
T4129 |
T4127 |
conj |
cytoplasm,nuclei |
R1859 |
T4130 |
T4131 |
punct |
(,shown |
R1860 |
T4131 |
T4119 |
parataxis |
shown,are |
R1861 |
T4132 |
T4131 |
nsubj |
data,shown |
R1862 |
T4133 |
T4131 |
neg |
not,shown |
R1863 |
T4134 |
T4131 |
punct |
),shown |
R1864 |
T4135 |
T4119 |
punct |
.,are |
R1865 |
T4137 |
T4138 |
det |
The,signal |
R1866 |
T4138 |
T4139 |
nsubj |
signal,are |
R1867 |
T4140 |
T4138 |
prep |
of,signal |
R1868 |
T4141 |
T4142 |
det |
the,border |
R1869 |
T4142 |
T4140 |
pobj |
border,of |
R1870 |
T4143 |
T4142 |
amod |
pial,border |
R1871 |
T4144 |
T4138 |
cc |
and,signal |
R1872 |
T4145 |
T4146 |
det |
the,stain |
R1873 |
T4146 |
T4138 |
conj |
stain,signal |
R1874 |
T4147 |
T4146 |
amod |
prominent,stain |
R1875 |
T4148 |
T4146 |
prep |
of,stain |
R1876 |
T4149 |
T4150 |
det |
the,tracts |
R1877 |
T4150 |
T4148 |
pobj |
tracts,of |
R1878 |
T4151 |
T4152 |
amod |
white,matter |
R1879 |
T4152 |
T4150 |
compound |
matter,tracts |
R1880 |
T4153 |
T4150 |
punct |
(,tracts |
R1881 |
T4154 |
T4155 |
compound |
laminae,medullares |
R1882 |
T4155 |
T4150 |
appos |
medullares,tracts |
R1883 |
T4156 |
T4139 |
punct |
),are |
R1884 |
T4157 |
T4139 |
prep |
due,are |
R1885 |
T4158 |
T4157 |
pcomp |
to,due |
R1886 |
T4159 |
T4160 |
compound |
Annexin,A7 |
R1887 |
T4160 |
T4161 |
npadvmod |
A7,positive |
R1888 |
T4161 |
T4162 |
amod |
positive,astrocytes |
R1889 |
T4162 |
T4157 |
pobj |
astrocytes,due |
R1890 |
T4163 |
T4164 |
punct |
(,A |
R1891 |
T4164 |
T4139 |
parataxis |
A,are |
R1892 |
T4165 |
T4164 |
nmod |
Fig.,A |
R1893 |
T4166 |
T4164 |
nummod |
5,A |
R1894 |
T4167 |
T4168 |
punct |
–,C |
R1895 |
T4168 |
T4164 |
prep |
C,A |
R1896 |
T4169 |
T4139 |
punct |
),are |
R1897 |
T4170 |
T4139 |
punct |
.,are |
R1898 |
T4172 |
T4173 |
amod |
Higher,magnification |
R1899 |
T4173 |
T4174 |
nsubj |
magnification,revealed |
R1900 |
T4175 |
T4173 |
prep |
of,magnification |
R1901 |
T4176 |
T4177 |
det |
the,layer |
R1902 |
T4177 |
T4175 |
pobj |
layer,of |
R1903 |
T4178 |
T4177 |
amod |
boundary,layer |
R1904 |
T4179 |
T4177 |
prep |
between,layer |
R1905 |
T4180 |
T4181 |
compound |
stratum,moleculare |
R1906 |
T4181 |
T4179 |
pobj |
moleculare,between |
R1907 |
T4182 |
T4181 |
cc |
and,moleculare |
R1908 |
T4183 |
T4181 |
conj |
granulosum,moleculare |
R1909 |
T4184 |
T4185 |
punct |
(,5D |
R1910 |
T4185 |
T4173 |
parataxis |
5D,magnification |
R1911 |
T4186 |
T4185 |
compound |
Fig.,5D |
R1912 |
T4187 |
T4185 |
punct |
),5D |
R1913 |
T4188 |
T4189 |
det |
an,signal |
R1914 |
T4189 |
T4174 |
dobj |
signal,revealed |
R1915 |
T4190 |
T4191 |
compound |
Annexin,A7 |
R1916 |
T4191 |
T4189 |
compound |
A7,signal |
R1917 |
T4192 |
T4189 |
prep |
in,signal |
R1918 |
T4193 |
T4192 |
pobj |
nuclei,in |
R1919 |
T4194 |
T4192 |
punct |
", ",in |
R1920 |
T4195 |
T4192 |
cc |
but,in |
R1921 |
T4196 |
T4195 |
advmod |
also,but |
R1922 |
T4197 |
T4192 |
conj |
in,in |
R1923 |
T4198 |
T4199 |
det |
the,cytoplasm |
R1924 |
T4199 |
T4197 |
pobj |
cytoplasm,in |
R1925 |
T4200 |
T4197 |
cc |
and,in |
R1926 |
T4201 |
T4197 |
conj |
at,in |
R1927 |
T4202 |
T4203 |
det |
the,membrane |
R1928 |
T4203 |
T4201 |
pobj |
membrane,at |
R1929 |
T4204 |
T4203 |
compound |
plasma,membrane |
R1930 |
T4205 |
T4203 |
prep |
of,membrane |
R1931 |
T4206 |
T4207 |
compound |
Purkinje,cells |
R1932 |
T4207 |
T4205 |
pobj |
cells,of |
R1933 |
T4208 |
T4209 |
punct |
(,5E |
R1934 |
T4209 |
T4174 |
parataxis |
5E,revealed |
R1935 |
T4210 |
T4209 |
compound |
Fig.,5E |
R1936 |
T4211 |
T4209 |
punct |
),5E |
R1937 |
T4212 |
T4174 |
punct |
.,revealed |
R1938 |
T4214 |
T4215 |
det |
The,pair |
R1939 |
T4215 |
T4217 |
nsubj |
pair,points |
R1940 |
T4216 |
T4215 |
amod |
typical,pair |
R1941 |
T4218 |
T4215 |
prep |
of,pair |
R1942 |
T4219 |
T4218 |
pobj |
dendrites,of |
R1943 |
T4220 |
T4217 |
prep |
to,points |
R1944 |
T4221 |
T4222 |
det |
the,margin |
R1945 |
T4222 |
T4220 |
pobj |
margin,to |
R1946 |
T4223 |
T4222 |
prep |
of,margin |
R1947 |
T4224 |
T4225 |
det |
the,cortex |
R1948 |
T4225 |
T4223 |
pobj |
cortex,of |
R1949 |
T4226 |
T4225 |
amod |
cerebellar,cortex |
R1950 |
T4227 |
T4217 |
punct |
.,points |
R1951 |
T4229 |
T4230 |
det |
The,specificity |
R1952 |
T4230 |
T4231 |
nsubjpass |
specificity,confirmed |
R1953 |
T4232 |
T4230 |
prep |
of,specificity |
R1954 |
T4233 |
T4234 |
det |
the,signal |
R1955 |
T4234 |
T4232 |
pobj |
signal,of |
R1956 |
T4235 |
T4236 |
compound |
Annexin,A7 |
R1957 |
T4236 |
T4234 |
compound |
A7,signal |
R1958 |
T4237 |
T4231 |
auxpass |
was,confirmed |
R1959 |
T4238 |
T4231 |
advmod |
further,confirmed |
R1960 |
T4239 |
T4231 |
prep |
in,confirmed |
R1961 |
T4240 |
T4241 |
amod |
similar,sections |
R1962 |
T4241 |
T4239 |
pobj |
sections,in |
R1963 |
T4242 |
T4241 |
compound |
brain,sections |
R1964 |
T4243 |
T4241 |
prep |
of,sections |
R1965 |
T4244 |
T4245 |
det |
the,mouse |
R1966 |
T4245 |
T4243 |
pobj |
mouse,of |
R1967 |
T4246 |
T4245 |
nmod |
AnxA7,mouse |
R1968 |
T4247 |
T4246 |
punct |
-,AnxA7 |
R1969 |
T4248 |
T4246 |
punct |
/,AnxA7 |
R1970 |
T4249 |
T4246 |
punct |
-,AnxA7 |
R1971 |
T4250 |
T4251 |
punct |
(,5F |
R1972 |
T4251 |
T4231 |
parataxis |
5F,confirmed |
R1973 |
T4252 |
T4251 |
compound |
Fig.,5F |
R1974 |
T4253 |
T4251 |
punct |
),5F |
R1975 |
T4254 |
T4231 |
punct |
.,confirmed |
R1976 |
T4256 |
T4257 |
compound |
Fig.,5G |
R1977 |
T4257 |
T4258 |
nsubj |
5G,shows |
R1978 |
T4259 |
T4260 |
compound |
Annexin,A7 |
R1979 |
T4260 |
T4258 |
dobj |
A7,shows |
R1980 |
T4261 |
T4258 |
prep |
in,shows |
R1981 |
T4262 |
T4261 |
pobj |
neurites,in |
R1982 |
T4263 |
T4262 |
punct |
(,neurites |
R1983 |
T4264 |
T4262 |
appos |
axons,neurites |
R1984 |
T4265 |
T4262 |
punct |
),neurites |
R1985 |
T4266 |
T4262 |
acl |
connecting,neurites |
R1986 |
T4267 |
T4268 |
det |
the,medullares |
R1987 |
T4268 |
T4266 |
dobj |
medullares,connecting |
R1988 |
T4269 |
T4268 |
compound |
laminae,medullares |
R1989 |
T4270 |
T4266 |
prep |
with,connecting |
R1990 |
T4271 |
T4272 |
det |
the,layer |
R1991 |
T4272 |
T4270 |
pobj |
layer,with |
R1992 |
T4273 |
T4274 |
compound |
Purkinje,cell |
R1993 |
T4274 |
T4272 |
compound |
cell,layer |
R1994 |
T4275 |
T4274 |
punct |
-,cell |
R1995 |
T4276 |
T4258 |
punct |
.,shows |
R1996 |
T4278 |
T4279 |
advmod |
Most,likely |
R1997 |
T4279 |
T4280 |
advmod |
likely,are |
R1998 |
T4281 |
T4280 |
nsubj |
these,are |
R1999 |
T4282 |
T4283 |
det |
the,axons |
R2000 |
T4283 |
T4280 |
attr |
axons,are |
R2001 |
T4284 |
T4283 |
prep |
of,axons |
R2002 |
T4285 |
T4286 |
det |
the,cells |
R2003 |
T4286 |
T4284 |
pobj |
cells,of |
R2004 |
T4287 |
T4286 |
compound |
Purkinje,cells |
R2005 |
T4288 |
T4286 |
punct |
-,cells |
R2006 |
T4289 |
T4280 |
punct |
.,are |
R2007 |
T4291 |
T4292 |
advmod |
Apart,contain |
R2008 |
T4293 |
T4291 |
prep |
from,Apart |
R2009 |
T4294 |
T4295 |
det |
these,neurites |
R2010 |
T4295 |
T4293 |
pobj |
neurites,from |
R2011 |
T4296 |
T4295 |
amod |
efferent,neurites |
R2012 |
T4297 |
T4298 |
det |
the,medullares |
R2013 |
T4298 |
T4292 |
nsubj |
medullares,contain |
R2014 |
T4299 |
T4298 |
compound |
laminae,medullares |
R2015 |
T4300 |
T4301 |
punct |
(,matter |
R2016 |
T4301 |
T4298 |
parataxis |
matter,medullares |
R2017 |
T4302 |
T4303 |
compound |
lamina,alba |
R2018 |
T4303 |
T4301 |
dep |
alba,matter |
R2019 |
T4304 |
T4301 |
punct |
", ",matter |
R2020 |
T4305 |
T4301 |
amod |
white,matter |
R2021 |
T4306 |
T4301 |
punct |
),matter |
R2022 |
T4307 |
T4308 |
amod |
afferent,fibers |
R2023 |
T4308 |
T4292 |
dobj |
fibers,contain |
R2024 |
T4309 |
T4308 |
amod |
mossy,fibers |
R2025 |
T4310 |
T4309 |
cc |
and,mossy |
R2026 |
T4311 |
T4309 |
conj |
climbing,mossy |
R2027 |
T4312 |
T4292 |
punct |
.,contain |
R2028 |
T4314 |
T4315 |
advmod |
Thus,arises |
R2029 |
T4316 |
T4315 |
punct |
", ",arises |
R2030 |
T4317 |
T4318 |
det |
the,stain |
R2031 |
T4318 |
T4315 |
nsubj |
stain,arises |
R2032 |
T4319 |
T4318 |
amod |
intense,stain |
R2033 |
T4320 |
T4318 |
prep |
of,stain |
R2034 |
T4321 |
T4322 |
det |
the,matter |
R2035 |
T4322 |
T4320 |
pobj |
matter,of |
R2036 |
T4323 |
T4322 |
amod |
cerebellar,matter |
R2037 |
T4324 |
T4322 |
amod |
white,matter |
R2038 |
T4325 |
T4315 |
prep |
from,arises |
R2039 |
T4326 |
T4327 |
compound |
Annexin,A7 |
R2040 |
T4327 |
T4325 |
pobj |
A7,from |
R2041 |
T4328 |
T4327 |
acl |
located,A7 |
R2042 |
T4329 |
T4328 |
prep |
in,located |
R2043 |
T4330 |
T4329 |
pobj |
astrocytes,in |
R2044 |
T4331 |
T4330 |
cc |
and,astrocytes |
R2045 |
T4332 |
T4330 |
conj |
neurites,astrocytes |
R2046 |
T4333 |
T4315 |
punct |
.,arises |
R2047 |
T4423 |
T4422 |
prep |
of,Expression |
R2048 |
T4424 |
T4425 |
compound |
Annexin,A7 |
R2049 |
T4425 |
T4423 |
pobj |
A7,of |
R2050 |
T4426 |
T4422 |
prep |
in,Expression |
R2051 |
T4427 |
T4428 |
det |
the,isocortex |
R2052 |
T4428 |
T4426 |
pobj |
isocortex,in |
R2053 |
T4429 |
T4430 |
amod |
adult,human |
R2054 |
T4430 |
T4428 |
amod |
human,isocortex |
R2055 |
T4432 |
T4433 |
prep |
In,exhibited |
R2056 |
T4434 |
T4435 |
det |
the,neocortex |
R2057 |
T4435 |
T4432 |
pobj |
neocortex,In |
R2058 |
T4436 |
T4435 |
amod |
human,neocortex |
R2059 |
T4437 |
T4435 |
amod |
parietal,neocortex |
R2060 |
T4438 |
T4435 |
prep |
of,neocortex |
R2061 |
T4439 |
T4440 |
amod |
aged,individuals |
R2062 |
T4440 |
T4438 |
pobj |
individuals,of |
R2063 |
T4441 |
T4440 |
prep |
without,individuals |
R2064 |
T4442 |
T4443 |
det |
any,alterations |
R2065 |
T4443 |
T4441 |
pobj |
alterations,without |
R2066 |
T4444 |
T4443 |
amod |
neuropathological,alterations |
R2067 |
T4445 |
T4433 |
punct |
", ",exhibited |
R2068 |
T4446 |
T4447 |
amod |
subpial,astrocytes |
R2069 |
T4447 |
T4433 |
nsubj |
astrocytes,exhibited |
R2070 |
T4448 |
T4449 |
det |
a,staining |
R2071 |
T4449 |
T4433 |
dobj |
staining,exhibited |
R2072 |
T4450 |
T4449 |
prep |
of,staining |
R2073 |
T4451 |
T4452 |
compound |
Annexin,A7 |
R2074 |
T4452 |
T4450 |
pobj |
A7,of |
R2075 |
T4453 |
T4433 |
prep |
in,exhibited |
R2076 |
T4454 |
T4455 |
det |
the,cytoplasm |
R2077 |
T4455 |
T4453 |
pobj |
cytoplasm,in |
R2078 |
T4456 |
T4433 |
punct |
.,exhibited |
R2079 |
T4458 |
T4459 |
det |
A,presence |
R2080 |
T4459 |
T4461 |
nsubjpass |
presence,limited |
R2081 |
T4460 |
T4459 |
amod |
nuclear,presence |
R2082 |
T4462 |
T4459 |
prep |
of,presence |
R2083 |
T4463 |
T4464 |
compound |
Annexin,A7 |
R2084 |
T4464 |
T4462 |
pobj |
A7,of |
R2085 |
T4465 |
T4461 |
auxpass |
was,limited |
R2086 |
T4466 |
T4461 |
prep |
to,limited |
R2087 |
T4467 |
T4468 |
amod |
single,astrocytes |
R2088 |
T4468 |
T4466 |
pobj |
astrocytes,to |
R2089 |
T4469 |
T4470 |
punct |
(,6B |
R2090 |
T4470 |
T4461 |
parataxis |
6B,limited |
R2091 |
T4471 |
T4470 |
compound |
Fig.,6B |
R2092 |
T4472 |
T4470 |
punct |
),6B |
R2093 |
T4473 |
T4461 |
punct |
.,limited |
R2094 |
T4475 |
T4476 |
amod |
Pyramidal,neurons |
R2095 |
T4476 |
T4477 |
nsubj |
neurons,exhibited |
R2096 |
T4478 |
T4476 |
punct |
", ",neurons |
R2097 |
T4479 |
T4480 |
advmod |
predominantly,those |
R2098 |
T4480 |
T4476 |
appos |
those,neurons |
R2099 |
T4481 |
T4480 |
prep |
of,those |
R2100 |
T4482 |
T4481 |
pobj |
layer,of |
R2101 |
T4483 |
T4482 |
nummod |
V,layer |
R2102 |
T4484 |
T4485 |
compound |
Annexin,A7 |
R2103 |
T4485 |
T4477 |
dobj |
A7,exhibited |
R2104 |
T4486 |
T4477 |
prep |
at,exhibited |
R2105 |
T4487 |
T4488 |
det |
the,membrane |
R2106 |
T4488 |
T4486 |
pobj |
membrane,at |
R2107 |
T4489 |
T4488 |
compound |
plasma,membrane |
R2108 |
T4490 |
T4488 |
prep |
of,membrane |
R2109 |
T4491 |
T4492 |
poss |
their,perikaryon |
R2110 |
T4492 |
T4490 |
pobj |
perikaryon,of |
R2111 |
T4493 |
T4494 |
advmod |
as,as |
R2112 |
T4494 |
T4490 |
cc |
as,of |
R2113 |
T4495 |
T4494 |
advmod |
well,as |
R2114 |
T4496 |
T4490 |
conj |
of,of |
R2115 |
T4497 |
T4498 |
det |
the,dendrite |
R2116 |
T4498 |
T4496 |
pobj |
dendrite,of |
R2117 |
T4499 |
T4498 |
amod |
apical,dendrite |
R2118 |
T4500 |
T4501 |
punct |
(,C |
R2119 |
T4501 |
T4477 |
parataxis |
C,exhibited |
R2120 |
T4502 |
T4501 |
nmod |
Fig.,C |
R2121 |
T4503 |
T4501 |
nmod |
6A,C |
R2122 |
T4504 |
T4501 |
punct |
",",C |
R2123 |
T4505 |
T4501 |
punct |
),C |
R2124 |
T4506 |
T4477 |
punct |
.,exhibited |
R2125 |
T4508 |
T4509 |
det |
The,neurons |
R2126 |
T4509 |
T4510 |
nsubj |
neurons,lacked |
R2127 |
T4511 |
T4512 |
det |
a,signal |
R2128 |
T4512 |
T4510 |
dobj |
signal,lacked |
R2129 |
T4513 |
T4512 |
prep |
for,signal |
R2130 |
T4514 |
T4515 |
compound |
Annexin,A7 |
R2131 |
T4515 |
T4513 |
pobj |
A7,for |
R2132 |
T4516 |
T4510 |
prep |
in,lacked |
R2133 |
T4517 |
T4518 |
poss |
their,nuclei |
R2134 |
T4518 |
T4516 |
pobj |
nuclei,in |
R2135 |
T4519 |
T4510 |
punct |
.,lacked |
R2136 |
T4521 |
T4522 |
amod |
Apical,dendrites |
R2137 |
T4522 |
T4523 |
nsubj |
dendrites,indicated |
R2138 |
T4524 |
T4522 |
prep |
within,dendrites |
R2139 |
T4525 |
T4526 |
det |
the,layer |
R2140 |
T4526 |
T4524 |
pobj |
layer,within |
R2141 |
T4527 |
T4526 |
amod |
molecular,layer |
R2142 |
T4528 |
T4523 |
advmod |
also,indicated |
R2143 |
T4529 |
T4530 |
det |
a,staining |
R2144 |
T4530 |
T4523 |
dobj |
staining,indicated |
R2145 |
T4531 |
T4530 |
amod |
positive,staining |
R2146 |
T4532 |
T4530 |
prep |
for,staining |
R2147 |
T4533 |
T4534 |
compound |
Annexin,A7 |
R2148 |
T4534 |
T4532 |
pobj |
A7,for |
R2149 |
T4535 |
T4536 |
punct |
(,6B |
R2150 |
T4536 |
T4523 |
parataxis |
6B,indicated |
R2151 |
T4537 |
T4536 |
compound |
Fig.,6B |
R2152 |
T4538 |
T4536 |
punct |
),6B |
R2153 |
T4539 |
T4523 |
punct |
.,indicated |
R2154 |
T4541 |
T4542 |
det |
The,pattern |
R2155 |
T4542 |
T4544 |
nsubj |
pattern,change |
R2156 |
T4543 |
T4542 |
compound |
staining,pattern |
R2157 |
T4545 |
T4542 |
prep |
of,pattern |
R2158 |
T4546 |
T4547 |
compound |
Annexin,A7 |
R2159 |
T4547 |
T4545 |
pobj |
A7,of |
R2160 |
T4548 |
T4542 |
prep |
in,pattern |
R2161 |
T4549 |
T4550 |
det |
the,brain |
R2162 |
T4550 |
T4548 |
pobj |
brain,in |
R2163 |
T4551 |
T4550 |
amod |
human,brain |
R2164 |
T4552 |
T4550 |
compound |
autopsy,brain |
R2165 |
T4553 |
T4544 |
aux |
did,change |
R2166 |
T4554 |
T4544 |
neg |
not,change |
R2167 |
T4555 |
T4544 |
prep |
after,change |
R2168 |
T4556 |
T4555 |
pobj |
pre-treatment,after |
R2169 |
T4557 |
T4556 |
prep |
with,pre-treatment |
R2170 |
T4558 |
T4557 |
pobj |
trypsin,with |
R2171 |
T4559 |
T4544 |
punct |
.,change |
R2180 |
T5137 |
T5136 |
prep |
of,Presence |
R2181 |
T5138 |
T5139 |
compound |
Annexin,A7 |
R2182 |
T5139 |
T5137 |
pobj |
A7,of |
R2183 |
T5140 |
T5136 |
prep |
in,Presence |
R2184 |
T5141 |
T5140 |
pobj |
nuclei,in |
R2185 |
T5142 |
T5141 |
prep |
from,nuclei |
R2186 |
T5143 |
T5144 |
amod |
neuronal,cells |
R2187 |
T5144 |
T5142 |
pobj |
cells,from |
R2188 |
T5145 |
T5143 |
cc |
and,neuronal |
R2189 |
T5146 |
T5143 |
conj |
astroglial,neuronal |
R2190 |
T5148 |
T5149 |
det |
The,localisation |
R2191 |
T5149 |
T5151 |
nsubjpass |
localisation,detected |
R2192 |
T5150 |
T5149 |
amod |
nuclear,localisation |
R2193 |
T5152 |
T5149 |
prep |
of,localisation |
R2194 |
T5153 |
T5154 |
compound |
Annexin,A7 |
R2195 |
T5154 |
T5152 |
pobj |
A7,of |
R2196 |
T5155 |
T5149 |
prep |
in,localisation |
R2197 |
T5156 |
T5155 |
pobj |
mice,in |
R2198 |
T5157 |
T5149 |
acl |
observed,localisation |
R2199 |
T5158 |
T5157 |
prep |
in,observed |
R2200 |
T5159 |
T5160 |
amod |
immature,cells |
R2201 |
T5160 |
T5158 |
pobj |
cells,in |
R2202 |
T5161 |
T5160 |
prep |
at,cells |
R2203 |
T5162 |
T5161 |
pobj |
E16,at |
R2204 |
T5163 |
T5160 |
cc |
and,cells |
R2205 |
T5164 |
T5165 |
amod |
differentiated,neurons |
R2206 |
T5165 |
T5160 |
conj |
neurons,cells |
R2207 |
T5166 |
T5165 |
amod |
adult,neurons |
R2208 |
T5167 |
T5151 |
aux |
could,detected |
R2209 |
T5168 |
T5151 |
auxpass |
be,detected |
R2210 |
T5169 |
T5151 |
advmod |
only,detected |
R2211 |
T5170 |
T5151 |
prep |
as,detected |
R2212 |
T5171 |
T5172 |
det |
a,signal |
R2213 |
T5172 |
T5170 |
pobj |
signal,as |
R2214 |
T5173 |
T5172 |
amod |
strong,signal |
R2215 |
T5174 |
T5175 |
advmod |
when,pre-treated |
R2216 |
T5175 |
T5151 |
advcl |
pre-treated,detected |
R2217 |
T5176 |
T5177 |
det |
the,sections |
R2218 |
T5177 |
T5175 |
nsubjpass |
sections,pre-treated |
R2219 |
T5178 |
T5177 |
compound |
brain,sections |
R2220 |
T5179 |
T5175 |
auxpass |
were,pre-treated |
R2221 |
T5180 |
T5175 |
prep |
with,pre-treated |
R2222 |
T5181 |
T5180 |
pobj |
trypsin,with |
R2223 |
T5182 |
T5175 |
prep |
before,pre-treated |
R2224 |
T5183 |
T5184 |
compound |
antibody,staining |
R2225 |
T5184 |
T5182 |
pobj |
staining,before |
R2226 |
T5185 |
T5151 |
punct |
.,detected |
R2227 |
T5187 |
T5188 |
det |
This,procedure |
R2228 |
T5188 |
T5189 |
nsubjpass |
procedure,thought |
R2229 |
T5190 |
T5189 |
auxpass |
is,thought |
R2230 |
T5191 |
T5192 |
aux |
to,allow |
R2231 |
T5192 |
T5189 |
xcomp |
allow,thought |
R2232 |
T5193 |
T5194 |
det |
the,antibodies |
R2233 |
T5194 |
T5195 |
nsubj |
antibodies,access |
R2234 |
T5195 |
T5192 |
ccomp |
access,allow |
R2235 |
T5196 |
T5195 |
aux |
to,access |
R2236 |
T5197 |
T5195 |
dobj |
epitopes,access |
R2237 |
T5198 |
T5197 |
acl |
masked,epitopes |
R2238 |
T5199 |
T5198 |
agent |
by,masked |
R2239 |
T5200 |
T5201 |
det |
the,fixation |
R2240 |
T5201 |
T5199 |
pobj |
fixation,by |
R2241 |
T5202 |
T5201 |
compound |
formaldehyde,fixation |
R2242 |
T5203 |
T5189 |
punct |
.,thought |
R2243 |
T5205 |
T5206 |
compound |
Methanol,fixation |
R2244 |
T5206 |
T5207 |
nsubj |
fixation,led |
R2245 |
T5208 |
T5206 |
prep |
of,fixation |
R2246 |
T5209 |
T5210 |
amod |
cultured,cells |
R2247 |
T5210 |
T5208 |
pobj |
cells,of |
R2248 |
T5211 |
T5207 |
prep |
to,led |
R2249 |
T5212 |
T5213 |
amod |
inconsistent,results |
R2250 |
T5213 |
T5211 |
pobj |
results,to |
R2251 |
T5214 |
T5207 |
punct |
.,led |
R2252 |
T5216 |
T5217 |
compound |
Antigen,retrieval |
R2253 |
T5217 |
T5218 |
nsubj |
retrieval,employs |
R2254 |
T5219 |
T5217 |
prep |
in,retrieval |
R2255 |
T5220 |
T5221 |
npadvmod |
formalin,fixed |
R2256 |
T5221 |
T5223 |
amod |
fixed,sections |
R2257 |
T5222 |
T5221 |
punct |
-,fixed |
R2258 |
T5223 |
T5219 |
pobj |
sections,in |
R2259 |
T5224 |
T5221 |
cc |
and,fixed |
R2260 |
T5225 |
T5226 |
npadvmod |
paraffin,embedded |
R2261 |
T5226 |
T5221 |
conj |
embedded,fixed |
R2262 |
T5227 |
T5226 |
punct |
-,embedded |
R2263 |
T5228 |
T5223 |
compound |
tissue,sections |
R2264 |
T5229 |
T5230 |
amod |
various,methods |
R2265 |
T5230 |
T5218 |
dobj |
methods,employs |
R2266 |
T5231 |
T5230 |
nmod |
heating,methods |
R2267 |
T5232 |
T5231 |
cc |
or,heating |
R2268 |
T5233 |
T5234 |
amod |
proteolytic,pre-treatment |
R2269 |
T5234 |
T5231 |
conj |
pre-treatment,heating |
R2270 |
T5235 |
T5218 |
punct |
[,employs |
R2271 |
T5236 |
T5237 |
punct |
[,15 |
R2272 |
T5237 |
T5218 |
parataxis |
15,employs |
R2273 |
T5238 |
T5239 |
punct |
-,17 |
R2274 |
T5239 |
T5237 |
prep |
17,15 |
R2275 |
T5240 |
T5237 |
punct |
],15 |
R2276 |
T5241 |
T5242 |
punct |
;,Ein |
R2277 |
T5242 |
T5218 |
meta |
Ein,employs |
R2278 |
T5243 |
T5242 |
nmod |
Handbuch,Ein |
R2279 |
T5244 |
T5242 |
nmod |
für,Ein |
R2280 |
T5245 |
T5242 |
nmod |
die,Ein |
R2281 |
T5246 |
T5242 |
nmod |
Histologie,Ein |
R2282 |
T5247 |
T5242 |
punct |
", ",Ein |
R2283 |
T5248 |
T5242 |
nmod |
dianova,Ein |
R2284 |
T5249 |
T5242 |
nmod |
GmbH,Ein |
R2285 |
T5250 |
T5242 |
punct |
", ",Ein |
R2286 |
T5251 |
T5242 |
nmod |
Hamburg,Ein |
R2287 |
T5252 |
T5242 |
punct |
", ",Ein |
R2288 |
T5253 |
T5242 |
nmod |
Germany,Ein |
R2289 |
T5254 |
T5242 |
punct |
],Ein |
R2290 |
T5255 |
T5218 |
punct |
.,employs |
R2291 |
T5257 |
T5258 |
det |
These,methods |
R2292 |
T5258 |
T5259 |
nsubj |
methods,result |
R2293 |
T5260 |
T5259 |
aux |
can,result |
R2294 |
T5261 |
T5259 |
prep |
in,result |
R2295 |
T5262 |
T5263 |
amod |
moderate,staining |
R2296 |
T5263 |
T5261 |
pobj |
staining,in |
R2297 |
T5264 |
T5262 |
cc |
or,moderate |
R2298 |
T5265 |
T5262 |
conj |
strong,moderate |
R2299 |
T5266 |
T5263 |
amod |
specific,staining |
R2300 |
T5267 |
T5263 |
compound |
antibody,staining |
R2301 |
T5268 |
T5259 |
punct |
", ",result |
R2302 |
T5269 |
T5259 |
cc |
but,result |
R2303 |
T5270 |
T5271 |
det |
the,detectability |
R2304 |
T5271 |
T5272 |
nsubjpass |
detectability,decreased |
R2305 |
T5272 |
T5259 |
conj |
decreased,result |
R2306 |
T5273 |
T5271 |
prep |
of,detectability |
R2307 |
T5274 |
T5275 |
amod |
other,antigens |
R2308 |
T5275 |
T5273 |
pobj |
antigens,of |
R2309 |
T5276 |
T5272 |
aux |
might,decreased |
R2310 |
T5277 |
T5272 |
auxpass |
be,decreased |
R2311 |
T5278 |
T5272 |
punct |
.,decreased |
R2312 |
T5280 |
T5281 |
advmod |
Moreover,has |
R2313 |
T5282 |
T5281 |
punct |
", ",has |
R2314 |
T5283 |
T5284 |
det |
the,pre-treatment |
R2315 |
T5284 |
T5281 |
nsubj |
pre-treatment,has |
R2316 |
T5285 |
T5284 |
amod |
optimal,pre-treatment |
R2317 |
T5286 |
T5287 |
aux |
to,individualised |
R2318 |
T5287 |
T5281 |
xcomp |
individualised,has |
R2319 |
T5288 |
T5287 |
auxpass |
be,individualised |
R2320 |
T5289 |
T5287 |
prep |
for,individualised |
R2321 |
T5290 |
T5291 |
det |
each,antigen |
R2322 |
T5291 |
T5289 |
pobj |
antigen,for |
R2323 |
T5292 |
T5281 |
punct |
.,has |
R2324 |
T5294 |
T5295 |
aux |
To,verify |
R2325 |
T5295 |
T5296 |
advcl |
verify,used |
R2326 |
T5297 |
T5298 |
det |
the,presence |
R2327 |
T5298 |
T5295 |
dobj |
presence,verify |
R2328 |
T5299 |
T5298 |
prep |
of,presence |
R2329 |
T5300 |
T5301 |
amod |
endogenous,A7 |
R2330 |
T5301 |
T5299 |
pobj |
A7,of |
R2331 |
T5302 |
T5301 |
compound |
Annexin,A7 |
R2332 |
T5303 |
T5295 |
prep |
in,verify |
R2333 |
T5304 |
T5303 |
pobj |
nuclei,in |
R2334 |
T5305 |
T5296 |
punct |
", ",used |
R2335 |
T5306 |
T5296 |
nsubj |
we,used |
R2336 |
T5307 |
T5308 |
det |
a,approach |
R2337 |
T5308 |
T5296 |
dobj |
approach,used |
R2338 |
T5309 |
T5308 |
amod |
biochemical,approach |
R2339 |
T5310 |
T5296 |
cc |
and,used |
R2340 |
T5311 |
T5296 |
conj |
purified,used |
R2341 |
T5312 |
T5311 |
dobj |
nuclei,purified |
R2342 |
T5313 |
T5311 |
prep |
from,purified |
R2343 |
T5314 |
T5315 |
nmod |
neuro,cells |
R2344 |
T5315 |
T5313 |
pobj |
cells,from |
R2345 |
T5316 |
T5314 |
punct |
-,neuro |
R2346 |
T5317 |
T5314 |
nummod |
2a,neuro |
R2347 |
T5318 |
T5314 |
punct |
", ",neuro |
R2348 |
T5319 |
T5314 |
conj |
PC,neuro |
R2349 |
T5320 |
T5319 |
punct |
-,PC |
R2350 |
T5321 |
T5319 |
nummod |
12,PC |
R2351 |
T5322 |
T5319 |
punct |
", ",PC |
R2352 |
T5323 |
T5319 |
cc |
and,PC |
R2353 |
T5324 |
T5319 |
conj |
C6,PC |
R2354 |
T5325 |
T5296 |
cc |
and,used |
R2355 |
T5326 |
T5296 |
conj |
treated,used |
R2356 |
T5327 |
T5326 |
dobj |
them,treated |
R2357 |
T5328 |
T5326 |
prep |
with,treated |
R2358 |
T5329 |
T5330 |
det |
a,buffer |
R2359 |
T5330 |
T5328 |
pobj |
buffer,with |
R2360 |
T5331 |
T5330 |
amod |
hypotonic,buffer |
R2361 |
T5332 |
T5330 |
compound |
extraction,buffer |
R2362 |
T5333 |
T5334 |
aux |
to,obtain |
R2363 |
T5334 |
T5326 |
advcl |
obtain,treated |
R2364 |
T5335 |
T5336 |
det |
the,nucleoplasm |
R2365 |
T5336 |
T5334 |
dobj |
nucleoplasm,obtain |
R2366 |
T5337 |
T5338 |
punct |
(,7A |
R2367 |
T5338 |
T5326 |
parataxis |
7A,treated |
R2368 |
T5339 |
T5338 |
compound |
Fig.,7A |
R2369 |
T5340 |
T5338 |
punct |
),7A |
R2370 |
T5341 |
T5296 |
punct |
.,used |
R2371 |
T5343 |
T5344 |
det |
The,nucleoplasm |
R2372 |
T5344 |
T5345 |
nsubjpass |
nucleoplasm,subjected |
R2373 |
T5346 |
T5344 |
cc |
and,nucleoplasm |
R2374 |
T5347 |
T5348 |
det |
the,nuclei |
R2375 |
T5348 |
T5344 |
conj |
nuclei,nucleoplasm |
R2376 |
T5349 |
T5348 |
amod |
remaining,nuclei |
R2377 |
T5350 |
T5344 |
punct |
", ",nucleoplasm |
R2378 |
T5351 |
T5352 |
prep |
from,extracted |
R2379 |
T5352 |
T5344 |
relcl |
extracted,nucleoplasm |
R2380 |
T5353 |
T5351 |
pobj |
which,from |
R2381 |
T5354 |
T5355 |
det |
the,nucleoplasm |
R2382 |
T5355 |
T5352 |
nsubjpass |
nucleoplasm,extracted |
R2383 |
T5356 |
T5352 |
aux |
has,extracted |
R2384 |
T5357 |
T5352 |
auxpass |
been,extracted |
R2385 |
T5358 |
T5352 |
advmod |
partially,extracted |
R2386 |
T5359 |
T5345 |
punct |
", ",subjected |
R2387 |
T5360 |
T5345 |
auxpass |
were,subjected |
R2388 |
T5361 |
T5345 |
prep |
to,subjected |
R2389 |
T5362 |
T5363 |
compound |
SDS,PAGE |
R2390 |
T5363 |
T5361 |
pobj |
PAGE,to |
R2391 |
T5364 |
T5363 |
punct |
-,PAGE |
R2392 |
T5365 |
T5363 |
cc |
and,PAGE |
R2393 |
T5366 |
T5367 |
compound |
Western,blotting |
R2394 |
T5367 |
T5363 |
conj |
blotting,PAGE |
R2395 |
T5368 |
T5345 |
punct |
.,subjected |
R2396 |
T5370 |
T5371 |
compound |
Western,blots |
R2397 |
T5371 |
T5372 |
nsubj |
blots,showed |
R2398 |
T5372 |
T5379 |
ccomp |
showed,extracted |
R2399 |
T5373 |
T5371 |
acl |
probed,blots |
R2400 |
T5374 |
T5373 |
prep |
with,probed |
R2401 |
T5375 |
T5376 |
nmod |
mAb,203 |
R2402 |
T5376 |
T5374 |
pobj |
203,with |
R2403 |
T5377 |
T5378 |
punct |
–,217 |
R2404 |
T5378 |
T5376 |
prep |
217,203 |
R2405 |
T5380 |
T5381 |
compound |
Annexin,A7 |
R2406 |
T5381 |
T5372 |
dobj |
A7,showed |
R2407 |
T5382 |
T5372 |
prep |
in,showed |
R2408 |
T5383 |
T5384 |
det |
the,nucleoplasm |
R2409 |
T5384 |
T5382 |
pobj |
nucleoplasm,in |
R2410 |
T5385 |
T5384 |
prep |
of,nucleoplasm |
R2411 |
T5386 |
T5387 |
amod |
neuronal,lines |
R2412 |
T5387 |
T5385 |
pobj |
lines,of |
R2413 |
T5388 |
T5389 |
punct |
(,2a |
R2414 |
T5389 |
T5386 |
parataxis |
2a,neuronal |
R2415 |
T5390 |
T5389 |
compound |
neuro,2a |
R2416 |
T5391 |
T5389 |
punct |
-,2a |
R2417 |
T5392 |
T5389 |
punct |
", ",2a |
R2418 |
T5393 |
T5389 |
appos |
PC,2a |
R2419 |
T5394 |
T5393 |
punct |
-,PC |
R2420 |
T5395 |
T5393 |
nummod |
12,PC |
R2421 |
T5396 |
T5389 |
punct |
),2a |
R2422 |
T5397 |
T5386 |
cc |
and,neuronal |
R2423 |
T5398 |
T5386 |
conj |
astroglial,neuronal |
R2424 |
T5399 |
T5400 |
punct |
(,C6 |
R2425 |
T5400 |
T5398 |
parataxis |
C6,astroglial |
R2426 |
T5401 |
T5400 |
punct |
),C6 |
R2427 |
T5402 |
T5387 |
compound |
cell,lines |
R2428 |
T5403 |
T5404 |
punct |
(,7A |
R2429 |
T5404 |
T5372 |
parataxis |
7A,showed |
R2430 |
T5405 |
T5404 |
compound |
Fig.,7A |
R2431 |
T5406 |
T5404 |
punct |
),7A |
R2432 |
T5407 |
T5379 |
punct |
", ",extracted |
R2433 |
T5408 |
T5379 |
advmod |
however,extracted |
R2434 |
T5409 |
T5379 |
punct |
", ",extracted |
R2435 |
T5410 |
T5411 |
det |
the,isoform |
R2436 |
T5411 |
T5379 |
nsubjpass |
isoform,extracted |
R2437 |
T5412 |
T5411 |
amod |
large,isoform |
R2438 |
T5413 |
T5379 |
aux |
could,extracted |
R2439 |
T5414 |
T5379 |
advmod |
only,extracted |
R2440 |
T5415 |
T5379 |
auxpass |
be,extracted |
R2441 |
T5416 |
T5379 |
advmod |
clearly,extracted |
R2442 |
T5417 |
T5379 |
prep |
with,extracted |
R2443 |
T5418 |
T5417 |
pobj |
nucleoplasm,with |
R2444 |
T5419 |
T5418 |
prep |
from,nucleoplasm |
R2445 |
T5420 |
T5421 |
compound |
C6,cells |
R2446 |
T5421 |
T5419 |
pobj |
cells,from |
R2447 |
T5422 |
T5379 |
punct |
.,extracted |
R2448 |
T5424 |
T5425 |
prep |
For,used |
R2449 |
T5426 |
T5424 |
pobj |
control,For |
R2450 |
T5427 |
T5425 |
punct |
", ",used |
R2451 |
T5428 |
T5425 |
nsubjpass |
antibodies,used |
R2452 |
T5429 |
T5428 |
prep |
against,antibodies |
R2453 |
T5430 |
T5429 |
pobj |
LAP2α,against |
R2454 |
T5431 |
T5430 |
punct |
", ",LAP2α |
R2455 |
T5432 |
T5430 |
conj |
Emerin,LAP2α |
R2456 |
T5433 |
T5432 |
cc |
and,Emerin |
R2457 |
T5434 |
T5432 |
conj |
tubulin,Emerin |
R2458 |
T5435 |
T5425 |
auxpass |
were,used |
R2459 |
T5436 |
T5437 |
aux |
to,verify |
R2460 |
T5437 |
T5425 |
advcl |
verify,used |
R2461 |
T5438 |
T5439 |
mark |
that,extracted |
R2462 |
T5439 |
T5437 |
ccomp |
extracted,verify |
R2463 |
T5440 |
T5441 |
det |
the,nucleoplasm |
R2464 |
T5441 |
T5439 |
nsubjpass |
nucleoplasm,extracted |
R2465 |
T5442 |
T5443 |
punct |
(,LAP2α |
R2466 |
T5443 |
T5441 |
parataxis |
LAP2α,nucleoplasm |
R2467 |
T5444 |
T5443 |
dep |
marker,LAP2α |
R2468 |
T5445 |
T5443 |
punct |
:,LAP2α |
R2469 |
T5446 |
T5443 |
punct |
", ",LAP2α |
R2470 |
T5447 |
T5448 |
dep |
which,is |
R2471 |
T5448 |
T5443 |
relcl |
is,LAP2α |
R2472 |
T5449 |
T5450 |
compound |
anon,membrane |
R2473 |
T5450 |
T5452 |
npadvmod |
membrane,bound |
R2474 |
T5451 |
T5450 |
punct |
-,membrane |
R2475 |
T5452 |
T5454 |
amod |
bound,isoform |
R2476 |
T5453 |
T5452 |
punct |
-,bound |
R2477 |
T5454 |
T5448 |
attr |
isoform,is |
R2478 |
T5455 |
T5454 |
prep |
of,isoform |
R2479 |
T5456 |
T5455 |
pobj |
LAP2,of |
R2480 |
T5457 |
T5443 |
punct |
),LAP2α |
R2481 |
T5458 |
T5439 |
auxpass |
was,extracted |
R2482 |
T5459 |
T5439 |
advmod |
successfully,extracted |
R2483 |
T5460 |
T5439 |
cc |
and,extracted |
R2484 |
T5461 |
T5460 |
advmod |
also,and |
R2485 |
T5462 |
T5463 |
neg |
not,contaminated |
R2486 |
T5463 |
T5439 |
conj |
contaminated,extracted |
R2487 |
T5464 |
T5463 |
prep |
by,contaminated |
R2488 |
T5465 |
T5466 |
amod |
nuclear,membranes |
R2489 |
T5466 |
T5464 |
pobj |
membranes,by |
R2490 |
T5467 |
T5468 |
punct |
(,Emerin |
R2491 |
T5468 |
T5466 |
parataxis |
Emerin,membranes |
R2492 |
T5469 |
T5468 |
dep |
marker,Emerin |
R2493 |
T5470 |
T5468 |
punct |
:,Emerin |
R2494 |
T5471 |
T5468 |
punct |
),Emerin |
R2495 |
T5472 |
T5464 |
cc |
or,by |
R2496 |
T5473 |
T5464 |
conj |
by,by |
R2497 |
T5474 |
T5473 |
pobj |
cytoplasm,by |
R2498 |
T5475 |
T5476 |
punct |
(,tubulin |
R2499 |
T5476 |
T5474 |
parataxis |
tubulin,cytoplasm |
R2500 |
T5477 |
T5476 |
dep |
marker,tubulin |
R2501 |
T5478 |
T5476 |
punct |
:,tubulin |
R2502 |
T5479 |
T5476 |
punct |
),tubulin |
R2503 |
T5480 |
T5425 |
punct |
.,used |
R2504 |
T5482 |
T5483 |
advmod |
Likewise,observed |
R2505 |
T5484 |
T5483 |
punct |
", ",observed |
R2506 |
T5485 |
T5483 |
prep |
in,observed |
R2507 |
T5486 |
T5487 |
det |
these,lines |
R2508 |
T5487 |
T5485 |
pobj |
lines,in |
R2509 |
T5488 |
T5487 |
amod |
neuronal,lines |
R2510 |
T5489 |
T5488 |
cc |
and,neuronal |
R2511 |
T5490 |
T5488 |
conj |
astroglial,neuronal |
R2512 |
T5491 |
T5487 |
compound |
cell,lines |
R2513 |
T5492 |
T5493 |
compound |
Annexin,A7 |
R2514 |
T5493 |
T5483 |
nsubjpass |
A7,observed |
R2515 |
T5494 |
T5483 |
aux |
had,observed |
R2516 |
T5495 |
T5483 |
auxpass |
been,observed |
R2517 |
T5496 |
T5483 |
prep |
in,observed |
R2518 |
T5497 |
T5498 |
det |
the,nucleus |
R2519 |
T5498 |
T5496 |
pobj |
nucleus,in |
R2520 |
T5499 |
T5483 |
prep |
by,observed |
R2521 |
T5500 |
T5499 |
pobj |
immunofluorescence,by |
R2522 |
T5501 |
T5502 |
punct |
(,7B |
R2523 |
T5502 |
T5483 |
parataxis |
7B,observed |
R2524 |
T5503 |
T5502 |
compound |
Fig.,7B |
R2525 |
T5504 |
T5502 |
cc |
and,7B |
R2526 |
T5505 |
T5506 |
nsubj |
data,shown |
R2527 |
T5506 |
T5502 |
conj |
shown,7B |
R2528 |
T5507 |
T5506 |
neg |
not,shown |
R2529 |
T5508 |
T5506 |
punct |
),shown |
R2530 |
T5509 |
T5483 |
punct |
.,observed |
R2531 |
T5511 |
T5512 |
nsubj |
We,noticed |
R2532 |
T5512 |
T5513 |
ccomp |
noticed,found |
R2533 |
T5514 |
T5515 |
det |
no,difference |
R2534 |
T5515 |
T5512 |
dobj |
difference,noticed |
R2535 |
T5516 |
T5515 |
prep |
between,difference |
R2536 |
T5517 |
T5518 |
nmod |
PC,cells |
R2537 |
T5518 |
T5516 |
pobj |
cells,between |
R2538 |
T5519 |
T5517 |
punct |
-,PC |
R2539 |
T5520 |
T5517 |
nummod |
12,PC |
R2540 |
T5521 |
T5517 |
cc |
and,PC |
R2541 |
T5522 |
T5523 |
compound |
neuro,2a |
R2542 |
T5523 |
T5517 |
conj |
2a,PC |
R2543 |
T5524 |
T5523 |
punct |
-,2a |
R2544 |
T5525 |
T5513 |
punct |
", ",found |
R2545 |
T5526 |
T5513 |
advmod |
however,found |
R2546 |
T5527 |
T5513 |
punct |
", ",found |
R2547 |
T5528 |
T5513 |
prep |
as,found |
R2548 |
T5529 |
T5528 |
prep |
for,as |
R2549 |
T5530 |
T5531 |
det |
the,sections |
R2550 |
T5531 |
T5529 |
pobj |
sections,for |
R2551 |
T5532 |
T5531 |
compound |
brain,sections |
R2552 |
T5533 |
T5513 |
punct |
", ",found |
R2553 |
T5534 |
T5513 |
nsubj |
we,found |
R2554 |
T5535 |
T5536 |
det |
an,increase |
R2555 |
T5536 |
T5513 |
dobj |
increase,found |
R2556 |
T5537 |
T5536 |
prep |
of,increase |
R2557 |
T5538 |
T5539 |
det |
the,staining |
R2558 |
T5539 |
T5537 |
pobj |
staining,of |
R2559 |
T5540 |
T5541 |
compound |
Annexin,A7 |
R2560 |
T5541 |
T5539 |
compound |
A7,staining |
R2561 |
T5542 |
T5513 |
prep |
after,found |
R2562 |
T5543 |
T5542 |
pobj |
pre-treatment,after |
R2563 |
T5544 |
T5543 |
prep |
with,pre-treatment |
R2564 |
T5545 |
T5544 |
pobj |
trypsin,with |
R2565 |
T5546 |
T5513 |
punct |
.,found |
R2596 |
T6416 |
T6417 |
prep |
In,explored |
R2597 |
T6418 |
T6419 |
det |
the,study |
R2598 |
T6419 |
T6416 |
pobj |
study,In |
R2599 |
T6420 |
T6419 |
amod |
present,study |
R2600 |
T6421 |
T6417 |
nsubj |
we,explored |
R2601 |
T6422 |
T6423 |
det |
the,appearance |
R2602 |
T6423 |
T6417 |
dobj |
appearance,explored |
R2603 |
T6424 |
T6423 |
prep |
of,appearance |
R2604 |
T6425 |
T6426 |
compound |
Annexin,A7 |
R2605 |
T6426 |
T6424 |
pobj |
A7,of |
R2606 |
T6427 |
T6417 |
prep |
during,explored |
R2607 |
T6428 |
T6429 |
compound |
mouse,development |
R2608 |
T6429 |
T6427 |
pobj |
development,during |
R2609 |
T6430 |
T6417 |
prep |
at,explored |
R2610 |
T6431 |
T6432 |
det |
the,level |
R2611 |
T6432 |
T6430 |
pobj |
level,at |
R2612 |
T6433 |
T6432 |
nmod |
mRNA,level |
R2613 |
T6434 |
T6433 |
cc |
and,mRNA |
R2614 |
T6435 |
T6433 |
conj |
protein,mRNA |
R2615 |
T6436 |
T6417 |
cc |
and,explored |
R2616 |
T6437 |
T6417 |
conj |
focused,explored |
R2617 |
T6438 |
T6437 |
prep |
on,focused |
R2618 |
T6439 |
T6440 |
det |
the,system |
R2619 |
T6440 |
T6438 |
pobj |
system,on |
R2620 |
T6441 |
T6440 |
amod |
central,system |
R2621 |
T6442 |
T6440 |
amod |
nervous,system |
R2622 |
T6443 |
T6437 |
prep |
during,focused |
R2623 |
T6444 |
T6443 |
pobj |
embryogenesis,during |
R2624 |
T6445 |
T6417 |
punct |
.,explored |
R2625 |
T6447 |
T6448 |
nmod |
Northern,blot |
R2626 |
T6448 |
T6449 |
nmod |
blot,analysis |
R2627 |
T6449 |
T6452 |
nsubj |
analysis,show |
R2628 |
T6450 |
T6448 |
cc |
and,blot |
R2629 |
T6451 |
T6448 |
conj |
immunohistochemistry,blot |
R2630 |
T6453 |
T6454 |
det |
the,expression |
R2631 |
T6454 |
T6452 |
dobj |
expression,show |
R2632 |
T6455 |
T6454 |
prep |
of,expression |
R2633 |
T6456 |
T6457 |
compound |
Annexin,A7 |
R2634 |
T6457 |
T6455 |
pobj |
A7,of |
R2635 |
T6458 |
T6452 |
prep |
in,show |
R2636 |
T6459 |
T6460 |
det |
all,tissues |
R2637 |
T6460 |
T6458 |
pobj |
tissues,in |
R2638 |
T6461 |
T6460 |
amod |
embryonic,tissues |
R2639 |
T6462 |
T6452 |
prep |
from,show |
R2640 |
T6463 |
T6464 |
compound |
day,E5 |
R2641 |
T6464 |
T6462 |
pobj |
E5,from |
R2642 |
T6465 |
T6464 |
advmod |
on,E5 |
R2643 |
T6466 |
T6464 |
punct |
", ",E5 |
R2644 |
T6467 |
T6468 |
det |
the,day |
R2645 |
T6468 |
T6464 |
appos |
day,E5 |
R2646 |
T6469 |
T6468 |
amod |
earliest,day |
R2647 |
T6470 |
T6468 |
acl |
studied,day |
R2648 |
T6471 |
T6452 |
punct |
.,show |
R2649 |
T6473 |
T6474 |
amod |
Differentiating,cells |
R2650 |
T6474 |
T6475 |
nsubj |
cells,show |
R2651 |
T6476 |
T6474 |
punct |
", ",cells |
R2652 |
T6477 |
T6474 |
prep |
like,cells |
R2653 |
T6478 |
T6479 |
det |
the,ectoderm |
R2654 |
T6479 |
T6477 |
pobj |
ectoderm,like |
R2655 |
T6480 |
T6479 |
amod |
neural,ectoderm |
R2656 |
T6481 |
T6479 |
punct |
", ",ectoderm |
R2657 |
T6482 |
T6483 |
dep |
which,is |
R2658 |
T6483 |
T6479 |
relcl |
is,ectoderm |
R2659 |
T6484 |
T6485 |
det |
the,origin |
R2660 |
T6485 |
T6483 |
attr |
origin,is |
R2661 |
T6486 |
T6485 |
prep |
of,origin |
R2662 |
T6487 |
T6488 |
det |
the,cells |
R2663 |
T6488 |
T6486 |
pobj |
cells,of |
R2664 |
T6489 |
T6488 |
acl |
belonging,cells |
R2665 |
T6490 |
T6489 |
prep |
to,belonging |
R2666 |
T6491 |
T6492 |
det |
the,system |
R2667 |
T6492 |
T6490 |
pobj |
system,to |
R2668 |
T6493 |
T6492 |
amod |
nervous,system |
R2669 |
T6494 |
T6475 |
punct |
", ",show |
R2670 |
T6495 |
T6496 |
compound |
Annexin,A7 |
R2671 |
T6496 |
T6497 |
compound |
A7,immunoreactivity |
R2672 |
T6497 |
T6475 |
dobj |
immunoreactivity,show |
R2673 |
T6498 |
T6499 |
advmod |
mainly,in |
R2674 |
T6499 |
T6475 |
prep |
in,show |
R2675 |
T6500 |
T6501 |
det |
the,cytosol |
R2676 |
T6501 |
T6499 |
pobj |
cytosol,in |
R2677 |
T6502 |
T6475 |
punct |
.,show |
R2678 |
T6504 |
T6505 |
amod |
Endodermal,cells |
R2679 |
T6505 |
T6510 |
nsubj |
cells,exhibit |
R2680 |
T6506 |
T6507 |
advmod |
as,as |
R2681 |
T6507 |
T6504 |
cc |
as,Endodermal |
R2682 |
T6508 |
T6507 |
advmod |
well,as |
R2683 |
T6509 |
T6504 |
conj |
mesodermal,Endodermal |
R2684 |
T6511 |
T6512 |
det |
a,localization |
R2685 |
T6512 |
T6510 |
dobj |
localization,exhibit |
R2686 |
T6513 |
T6512 |
amod |
similar,localization |
R2687 |
T6514 |
T6512 |
amod |
subcellular,localization |
R2688 |
T6515 |
T6512 |
prep |
of,localization |
R2689 |
T6516 |
T6517 |
det |
the,protein |
R2690 |
T6517 |
T6515 |
pobj |
protein,of |
R2691 |
T6518 |
T6510 |
punct |
.,exhibit |
R2692 |
T6520 |
T6521 |
prep |
In,noted |
R2693 |
T6522 |
T6523 |
det |
the,brain |
R2694 |
T6523 |
T6520 |
pobj |
brain,In |
R2695 |
T6524 |
T6523 |
amod |
developing,brain |
R2696 |
T6525 |
T6521 |
nsubj |
we,noted |
R2697 |
T6526 |
T6527 |
det |
a,change |
R2698 |
T6527 |
T6521 |
dobj |
change,noted |
R2699 |
T6528 |
T6527 |
amod |
striking,change |
R2700 |
T6529 |
T6527 |
prep |
in,change |
R2701 |
T6530 |
T6531 |
det |
the,distribution |
R2702 |
T6531 |
T6529 |
pobj |
distribution,in |
R2703 |
T6532 |
T6531 |
amod |
subcellular,distribution |
R2704 |
T6533 |
T6531 |
prep |
of,distribution |
R2705 |
T6534 |
T6535 |
compound |
Annexin,A7 |
R2706 |
T6535 |
T6533 |
pobj |
A7,of |
R2707 |
T6536 |
T6521 |
punct |
.,noted |
R2708 |
T6538 |
T6539 |
nsubj |
Cells,show |
R2709 |
T6540 |
T6538 |
prep |
in,Cells |
R2710 |
T6541 |
T6542 |
det |
the,germinativum |
R2711 |
T6542 |
T6540 |
pobj |
germinativum,in |
R2712 |
T6543 |
T6542 |
compound |
stratum,germinativum |
R2713 |
T6544 |
T6542 |
prep |
of,germinativum |
R2714 |
T6545 |
T6546 |
det |
the,cortex |
R2715 |
T6546 |
T6544 |
pobj |
cortex,of |
R2716 |
T6547 |
T6546 |
amod |
neopallial,cortex |
R2717 |
T6548 |
T6546 |
punct |
", ",cortex |
R2718 |
T6549 |
T6550 |
dep |
which,surrounds |
R2719 |
T6550 |
T6546 |
relcl |
surrounds,cortex |
R2720 |
T6551 |
T6552 |
det |
the,ventricle |
R2721 |
T6552 |
T6550 |
dobj |
ventricle,surrounds |
R2722 |
T6553 |
T6552 |
amod |
lateral,ventricle |
R2723 |
T6554 |
T6539 |
punct |
", ",show |
R2724 |
T6555 |
T6539 |
prep |
at,show |
R2725 |
T6556 |
T6555 |
pobj |
E13,at |
R2726 |
T6557 |
T6556 |
cc |
and,E13 |
R2727 |
T6558 |
T6556 |
conj |
E15,E13 |
R2728 |
T6559 |
T6560 |
det |
a,staining |
R2729 |
T6560 |
T6539 |
dobj |
staining,show |
R2730 |
T6561 |
T6560 |
prep |
for,staining |
R2731 |
T6562 |
T6563 |
compound |
Annexin,A7 |
R2732 |
T6563 |
T6561 |
pobj |
A7,for |
R2733 |
T6564 |
T6565 |
advmod |
mainly,in |
R2734 |
T6565 |
T6539 |
prep |
in,show |
R2735 |
T6566 |
T6567 |
det |
the,cytosol |
R2736 |
T6567 |
T6565 |
pobj |
cytosol,in |
R2737 |
T6568 |
T6539 |
punct |
.,show |
R2738 |
T6570 |
T6571 |
cc |
But,disappears |
R2739 |
T6572 |
T6573 |
det |
this,staining |
R2740 |
T6573 |
T6571 |
nsubj |
staining,disappears |
R2741 |
T6574 |
T6571 |
advmod |
largely,disappears |
R2742 |
T6575 |
T6571 |
cc |
and,disappears |
R2743 |
T6576 |
T6577 |
prep |
at,detect |
R2744 |
T6577 |
T6571 |
conj |
detect,disappears |
R2745 |
T6578 |
T6579 |
det |
the,day |
R2746 |
T6579 |
T6576 |
pobj |
day,at |
R2747 |
T6580 |
T6579 |
amod |
following,day |
R2748 |
T6581 |
T6579 |
appos |
E16,day |
R2749 |
T6582 |
T6577 |
nsubj |
we,detect |
R2750 |
T6583 |
T6584 |
compound |
Annexin,A7 |
R2751 |
T6584 |
T6577 |
dobj |
A7,detect |
R2752 |
T6585 |
T6577 |
prep |
in,detect |
R2753 |
T6586 |
T6587 |
det |
the,nucleus |
R2754 |
T6587 |
T6585 |
pobj |
nucleus,in |
R2755 |
T6588 |
T6587 |
prep |
of,nucleus |
R2756 |
T6589 |
T6588 |
pobj |
cells,of |
R2757 |
T6590 |
T6577 |
prep |
in,detect |
R2758 |
T6591 |
T6592 |
det |
the,zone |
R2759 |
T6592 |
T6590 |
pobj |
zone,in |
R2760 |
T6593 |
T6592 |
amod |
intermediate,zone |
R2761 |
T6594 |
T6593 |
cc |
and,intermediate |
R2762 |
T6595 |
T6593 |
conj |
marginal,intermediate |
R2763 |
T6596 |
T6592 |
prep |
of,zone |
R2764 |
T6597 |
T6598 |
det |
the,neopallium |
R2765 |
T6598 |
T6596 |
pobj |
neopallium,of |
R2766 |
T6599 |
T6577 |
punct |
.,detect |
R2767 |
T6601 |
T6602 |
det |
The,patterns |
R2768 |
T6602 |
T6605 |
nsubj |
patterns,are |
R2769 |
T6603 |
T6602 |
amod |
different,patterns |
R2770 |
T6604 |
T6602 |
compound |
expression,patterns |
R2771 |
T6606 |
T6602 |
prep |
of,patterns |
R2772 |
T6607 |
T6608 |
compound |
Annexin,A7 |
R2773 |
T6608 |
T6609 |
npadvmod |
A7,positive |
R2774 |
T6609 |
T6611 |
amod |
positive,cells |
R2775 |
T6610 |
T6609 |
punct |
-,positive |
R2776 |
T6611 |
T6606 |
pobj |
cells,of |
R2777 |
T6612 |
T6602 |
prep |
from,patterns |
R2778 |
T6613 |
T6614 |
det |
the,zone |
R2779 |
T6614 |
T6612 |
pobj |
zone,from |
R2780 |
T6615 |
T6614 |
amod |
ventricular,zone |
R2781 |
T6616 |
T6614 |
amod |
germinative,zone |
R2782 |
T6617 |
T6612 |
prep |
to,from |
R2783 |
T6618 |
T6619 |
det |
the,zone |
R2784 |
T6619 |
T6617 |
pobj |
zone,to |
R2785 |
T6620 |
T6619 |
amod |
marginal,zone |
R2786 |
T6621 |
T6612 |
prep |
of,from |
R2787 |
T6622 |
T6623 |
det |
the,neocortex |
R2788 |
T6623 |
T6621 |
pobj |
neocortex,of |
R2789 |
T6624 |
T6623 |
amod |
later,neocortex |
R2790 |
T6625 |
T6602 |
acl |
observed,patterns |
R2791 |
T6626 |
T6625 |
prep |
in,observed |
R2792 |
T6627 |
T6628 |
det |
this,study |
R2793 |
T6628 |
T6626 |
pobj |
study,in |
R2794 |
T6629 |
T6605 |
acomp |
similar,are |
R2795 |
T6630 |
T6629 |
prep |
to,similar |
R2796 |
T6631 |
T6632 |
det |
the,patterns |
R2797 |
T6632 |
T6630 |
pobj |
patterns,to |
R2798 |
T6633 |
T6632 |
amod |
developmental,patterns |
R2799 |
T6634 |
T6632 |
prep |
of,patterns |
R2800 |
T6635 |
T6636 |
compound |
Tenascin,C |
R2801 |
T6636 |
T6638 |
npadvmod |
C,positive |
R2802 |
T6637 |
T6636 |
punct |
-,C |
R2803 |
T6638 |
T6640 |
amod |
positive,precursors |
R2804 |
T6639 |
T6638 |
punct |
-,positive |
R2805 |
T6640 |
T6634 |
pobj |
precursors,of |
R2806 |
T6641 |
T6640 |
amod |
astroglial,precursors |
R2807 |
T6642 |
T6640 |
acl |
following,precursors |
R2808 |
T6643 |
T6644 |
det |
the,guidance |
R2809 |
T6644 |
T6642 |
dobj |
guidance,following |
R2810 |
T6645 |
T6644 |
prep |
of,guidance |
R2811 |
T6646 |
T6647 |
det |
the,cells |
R2812 |
T6647 |
T6645 |
pobj |
cells,of |
R2813 |
T6648 |
T6647 |
amod |
radial,cells |
R2814 |
T6649 |
T6647 |
amod |
glial,cells |
R2815 |
T6650 |
T6651 |
punct |
[,18 |
R2816 |
T6651 |
T6605 |
parataxis |
18,are |
R2817 |
T6652 |
T6651 |
punct |
],18 |
R2818 |
T6653 |
T6605 |
punct |
.,are |
R2819 |
T6655 |
T6656 |
advmod |
Moreover,resemble |
R2820 |
T6657 |
T6656 |
punct |
", ",resemble |
R2821 |
T6658 |
T6659 |
det |
the,patterns |
R2822 |
T6659 |
T6656 |
nsubj |
patterns,resemble |
R2823 |
T6660 |
T6656 |
dobj |
that,resemble |
R2824 |
T6661 |
T6660 |
prep |
of,that |
R2825 |
T6662 |
T6663 |
amod |
migrating,neurons |
R2826 |
T6663 |
T6661 |
pobj |
neurons,of |
R2827 |
T6664 |
T6662 |
cc |
and,migrating |
R2828 |
T6665 |
T6662 |
conj |
differentiated,migrating |
R2829 |
T6666 |
T6663 |
acl |
described,neurons |
R2830 |
T6667 |
T6666 |
agent |
by,described |
R2831 |
T6668 |
T6667 |
pobj |
Berry,by |
R2832 |
T6669 |
T6670 |
advmod |
et,al. |
R2833 |
T6670 |
T6668 |
advmod |
al.,Berry |
R2834 |
T6671 |
T6672 |
punct |
[,13 |
R2835 |
T6672 |
T6656 |
parataxis |
13,resemble |
R2836 |
T6673 |
T6672 |
punct |
],13 |
R2837 |
T6674 |
T6656 |
punct |
.,resemble |
R2838 |
T6676 |
T6677 |
nsubj |
Neurons,migrate |
R2839 |
T6678 |
T6679 |
dep |
that,generated |
R2840 |
T6679 |
T6676 |
relcl |
generated,Neurons |
R2841 |
T6680 |
T6679 |
auxpass |
are,generated |
R2842 |
T6681 |
T6679 |
advmod |
prenatally,generated |
R2843 |
T6682 |
T6679 |
prep |
in,generated |
R2844 |
T6683 |
T6684 |
det |
the,layer |
R2845 |
T6684 |
T6682 |
pobj |
layer,in |
R2846 |
T6685 |
T6684 |
amod |
proliferative,layer |
R2847 |
T6686 |
T6684 |
amod |
ventricular,layer |
R2848 |
T6687 |
T6684 |
prep |
of,layer |
R2849 |
T6688 |
T6689 |
det |
the,cortex |
R2850 |
T6689 |
T6687 |
pobj |
cortex,of |
R2851 |
T6690 |
T6689 |
amod |
neopallial,cortex |
R2852 |
T6691 |
T6677 |
advmod |
subsequently,migrate |
R2853 |
T6692 |
T6677 |
prep |
through,migrate |
R2854 |
T6693 |
T6694 |
det |
the,zone |
R2855 |
T6694 |
T6692 |
pobj |
zone,through |
R2856 |
T6695 |
T6694 |
amod |
intermediate,zone |
R2857 |
T6696 |
T6697 |
aux |
to,form |
R2858 |
T6697 |
T6677 |
advcl |
form,migrate |
R2859 |
T6698 |
T6699 |
det |
the,layers |
R2860 |
T6699 |
T6697 |
dobj |
layers,form |
R2861 |
T6700 |
T6699 |
amod |
different,layers |
R2862 |
T6701 |
T6699 |
amod |
cortical,layers |
R2863 |
T6702 |
T6699 |
compound |
cell,layers |
R2864 |
T6703 |
T6697 |
prep |
in,form |
R2865 |
T6704 |
T6705 |
det |
a,gradient |
R2866 |
T6705 |
T6703 |
pobj |
gradient,in |
R2867 |
T6706 |
T6705 |
amod |
declining,gradient |
R2868 |
T6707 |
T6708 |
amod |
inside,out |
R2869 |
T6708 |
T6705 |
amod |
out,gradient |
R2870 |
T6709 |
T6708 |
punct |
-,out |
R2871 |
T6710 |
T6705 |
prep |
of,gradient |
R2872 |
T6711 |
T6712 |
compound |
cell,maturation |
R2873 |
T6712 |
T6710 |
pobj |
maturation,of |
R2874 |
T6713 |
T6677 |
punct |
.,migrate |
R2875 |
T6715 |
T6716 |
det |
These,observations |
R2876 |
T6716 |
T6717 |
nsubj |
observations,suggested |
R2877 |
T6718 |
T6719 |
mark |
that,depends |
R2878 |
T6719 |
T6717 |
ccomp |
depends,suggested |
R2879 |
T6720 |
T6721 |
det |
the,localization |
R2880 |
T6721 |
T6719 |
nsubj |
localization,depends |
R2881 |
T6722 |
T6721 |
amod |
subcellular,localization |
R2882 |
T6723 |
T6721 |
prep |
of,localization |
R2883 |
T6724 |
T6725 |
compound |
Annexin,A7 |
R2884 |
T6725 |
T6723 |
pobj |
A7,of |
R2885 |
T6726 |
T6719 |
prep |
on,depends |
R2886 |
T6727 |
T6728 |
amod |
developmental,stage |
R2887 |
T6728 |
T6726 |
pobj |
stage,on |
R2888 |
T6729 |
T6728 |
cc |
and,stage |
R2889 |
T6730 |
T6731 |
compound |
cell,type |
R2890 |
T6731 |
T6728 |
conj |
type,stage |
R2891 |
T6732 |
T6717 |
punct |
.,suggested |
R2892 |
T6734 |
T6735 |
prep |
In,observed |
R2893 |
T6736 |
T6737 |
det |
the,brain |
R2894 |
T6737 |
T6734 |
pobj |
brain,In |
R2895 |
T6738 |
T6737 |
amod |
adult,brain |
R2896 |
T6739 |
T6735 |
nsubj |
we,observed |
R2897 |
T6740 |
T6735 |
advmod |
generally,observed |
R2898 |
T6741 |
T6742 |
det |
a,localization |
R2899 |
T6742 |
T6735 |
dobj |
localization,observed |
R2900 |
T6743 |
T6742 |
amod |
nuclear,localization |
R2901 |
T6744 |
T6735 |
prep |
for,observed |
R2902 |
T6745 |
T6746 |
compound |
Annexin,A7 |
R2903 |
T6746 |
T6744 |
pobj |
A7,for |
R2904 |
T6747 |
T6735 |
prep |
in,observed |
R2905 |
T6748 |
T6747 |
pobj |
neurons,in |
R2906 |
T6749 |
T6750 |
mark |
whereas,exhibited |
R2907 |
T6750 |
T6735 |
advcl |
exhibited,observed |
R2908 |
T6751 |
T6750 |
nsubj |
astrocytes,exhibited |
R2909 |
T6752 |
T6753 |
preconj |
both,cytosolic |
R2910 |
T6753 |
T6750 |
dobj |
cytosolic,exhibited |
R2911 |
T6754 |
T6753 |
det |
a,cytosolic |
R2912 |
T6755 |
T6756 |
advmod |
as,as |
R2913 |
T6756 |
T6753 |
cc |
as,cytosolic |
R2914 |
T6757 |
T6756 |
advmod |
well,as |
R2915 |
T6758 |
T6759 |
det |
a,nuclear |
R2916 |
T6759 |
T6760 |
nmod |
nuclear,staining |
R2917 |
T6760 |
T6753 |
conj |
staining,cytosolic |
R2918 |
T6761 |
T6735 |
punct |
.,observed |
R2919 |
T6763 |
T6764 |
advmod |
However,exhibited |
R2920 |
T6765 |
T6764 |
punct |
", ",exhibited |
R2921 |
T6766 |
T6767 |
amod |
pyramidal,neurons |
R2922 |
T6767 |
T6764 |
nsubj |
neurons,exhibited |
R2923 |
T6768 |
T6767 |
prep |
of,neurons |
R2924 |
T6769 |
T6770 |
det |
the,isocortex |
R2925 |
T6770 |
T6768 |
pobj |
isocortex,of |
R2926 |
T6771 |
T6767 |
cc |
and,neurons |
R2927 |
T6772 |
T6773 |
compound |
Purkinje,cells |
R2928 |
T6773 |
T6767 |
conj |
cells,neurons |
R2929 |
T6774 |
T6773 |
punct |
-,cells |
R2930 |
T6775 |
T6773 |
prep |
of,cells |
R2931 |
T6776 |
T6777 |
det |
the,cerebellum |
R2932 |
T6777 |
T6775 |
pobj |
cerebellum,of |
R2933 |
T6778 |
T6779 |
det |
a,stain |
R2934 |
T6779 |
T6764 |
dobj |
stain,exhibited |
R2935 |
T6780 |
T6779 |
amod |
cytosolic,stain |
R2936 |
T6781 |
T6779 |
prep |
of,stain |
R2937 |
T6782 |
T6783 |
amod |
intermediate,intensity |
R2938 |
T6783 |
T6781 |
pobj |
intensity,of |
R2939 |
T6784 |
T6764 |
prep |
including,exhibited |
R2940 |
T6785 |
T6786 |
poss |
their,neurites |
R2941 |
T6786 |
T6784 |
pobj |
neurites,including |
R2942 |
T6787 |
T6786 |
cc |
and,neurites |
R2943 |
T6788 |
T6786 |
conj |
dendrites,neurites |
R2944 |
T6789 |
T6764 |
punct |
.,exhibited |
R2945 |
T6791 |
T6792 |
nsubj |
We,reported |
R2946 |
T6793 |
T6792 |
aux |
have,reported |
R2947 |
T6794 |
T6792 |
advmod |
previously,reported |
R2948 |
T6795 |
T6796 |
det |
the,expression |
R2949 |
T6796 |
T6792 |
dobj |
expression,reported |
R2950 |
T6797 |
T6796 |
prep |
of,expression |
R2951 |
T6798 |
T6799 |
compound |
Annexin,A7 |
R2952 |
T6799 |
T6797 |
pobj |
A7,of |
R2953 |
T6800 |
T6796 |
prep |
in,expression |
R2954 |
T6801 |
T6802 |
amod |
human,neocortex |
R2955 |
T6802 |
T6800 |
pobj |
neocortex,in |
R2956 |
T6803 |
T6802 |
amod |
temporal,neocortex |
R2957 |
T6804 |
T6802 |
cc |
and,neocortex |
R2958 |
T6805 |
T6802 |
conj |
hippocampus,neocortex |
R2959 |
T6806 |
T6802 |
acl |
obtained,neocortex |
R2960 |
T6807 |
T6806 |
prep |
from,obtained |
R2961 |
T6808 |
T6807 |
pobj |
neurosurgery,from |
R2962 |
T6809 |
T6808 |
prep |
for,neurosurgery |
R2963 |
T6810 |
T6811 |
npadvmod |
therapy,refractory |
R2964 |
T6811 |
T6813 |
amod |
refractory,epilepsy |
R2965 |
T6812 |
T6811 |
punct |
-,refractory |
R2966 |
T6813 |
T6809 |
pobj |
epilepsy,for |
R2967 |
T6814 |
T6792 |
cc |
and,reported |
R2968 |
T6815 |
T6792 |
conj |
found,reported |
R2969 |
T6816 |
T6817 |
det |
the,isoforms |
R2970 |
T6817 |
T6815 |
dobj |
isoforms,found |
R2971 |
T6818 |
T6817 |
nummod |
two,isoforms |
R2972 |
T6819 |
T6820 |
compound |
Annexin,A7 |
R2973 |
T6820 |
T6817 |
compound |
A7,isoforms |
R2974 |
T6821 |
T6817 |
acl |
restricted,isoforms |
R2975 |
T6822 |
T6821 |
prep |
to,restricted |
R2976 |
T6823 |
T6824 |
det |
the,cytoplasm |
R2977 |
T6824 |
T6822 |
pobj |
cytoplasm,to |
R2978 |
T6825 |
T6824 |
cc |
and,cytoplasm |
R2979 |
T6826 |
T6824 |
conj |
nuclei,cytoplasm |
R2980 |
T6827 |
T6824 |
prep |
of,cytoplasm |
R2981 |
T6828 |
T6827 |
pobj |
astrocytes,of |
R2982 |
T6829 |
T6815 |
punct |
", ",found |
R2983 |
T6830 |
T6831 |
mark |
whereas,lacked |
R2984 |
T6831 |
T6815 |
advcl |
lacked,found |
R2985 |
T6832 |
T6831 |
nsubj |
neurons,lacked |
R2986 |
T6833 |
T6834 |
det |
any,signal |
R2987 |
T6834 |
T6831 |
dobj |
signal,lacked |
R2988 |
T6835 |
T6836 |
punct |
[,19 |
R2989 |
T6836 |
T6815 |
parataxis |
19,found |
R2990 |
T6837 |
T6836 |
punct |
],19 |
R2991 |
T6838 |
T6792 |
punct |
.,reported |
R2992 |
T6840 |
T6841 |
det |
The,area |
R2993 |
T6841 |
T6843 |
nsubj |
area,showed |
R2994 |
T6842 |
T6841 |
amod |
hippocampal,area |
R2995 |
T6844 |
T6845 |
amod |
typical,signs |
R2996 |
T6845 |
T6843 |
dobj |
signs,showed |
R2997 |
T6846 |
T6845 |
prep |
of,signs |
R2998 |
T6847 |
T6848 |
poss |
Ammon,sclerosis |
R2999 |
T6848 |
T6846 |
pobj |
sclerosis,of |
R3000 |
T6849 |
T6847 |
case |
's,Ammon |
R3001 |
T6850 |
T6848 |
compound |
horn,sclerosis |
R3002 |
T6851 |
T6843 |
punct |
", ",showed |
R3003 |
T6852 |
T6843 |
cc |
but,showed |
R3004 |
T6853 |
T6854 |
det |
the,tissue |
R3005 |
T6854 |
T6858 |
nsubj |
tissue,show |
R3006 |
T6855 |
T6854 |
amod |
adjacent,tissue |
R3007 |
T6856 |
T6857 |
amod |
temporal,neocortex |
R3008 |
T6857 |
T6854 |
compound |
neocortex,tissue |
R3009 |
T6858 |
T6843 |
conj |
show,showed |
R3010 |
T6859 |
T6858 |
aux |
did,show |
R3011 |
T6860 |
T6858 |
neg |
not,show |
R3012 |
T6861 |
T6862 |
det |
any,alterations |
R3013 |
T6862 |
T6858 |
dobj |
alterations,show |
R3014 |
T6863 |
T6862 |
amod |
histopathological,alterations |
R3015 |
T6864 |
T6858 |
punct |
.,show |
R3016 |
T6866 |
T6867 |
det |
This,data |
R3017 |
T6867 |
T6868 |
nsubj |
data,is |
R3018 |
T6869 |
T6868 |
prep |
in,is |
R3019 |
T6870 |
T6869 |
pobj |
discrepancy,in |
R3020 |
T6871 |
T6870 |
prep |
with,discrepancy |
R3021 |
T6872 |
T6873 |
poss |
our,observation |
R3022 |
T6873 |
T6871 |
pobj |
observation,with |
R3023 |
T6874 |
T6873 |
amod |
actual,observation |
R3024 |
T6875 |
T6868 |
prep |
in,is |
R3025 |
T6876 |
T6877 |
det |
the,brain |
R3026 |
T6877 |
T6875 |
pobj |
brain,in |
R3027 |
T6878 |
T6877 |
amod |
murine,brain |
R3028 |
T6879 |
T6868 |
punct |
.,is |
R3029 |
T6881 |
T6882 |
aux |
To,determine |
R3030 |
T6882 |
T6883 |
advcl |
determine,repeated |
R3031 |
T6884 |
T6885 |
mark |
if,is |
R3032 |
T6885 |
T6882 |
ccomp |
is,determine |
R3033 |
T6886 |
T6885 |
nsubj |
this,is |
R3034 |
T6887 |
T6885 |
prep |
due,is |
R3035 |
T6888 |
T6887 |
pcomp |
to,due |
R3036 |
T6889 |
T6890 |
amod |
different,methods |
R3037 |
T6890 |
T6887 |
pobj |
methods,due |
R3038 |
T6891 |
T6890 |
prep |
in,methods |
R3039 |
T6892 |
T6893 |
compound |
immunofluorescence,staining |
R3040 |
T6893 |
T6891 |
pobj |
staining,in |
R3041 |
T6894 |
T6890 |
cc |
or,methods |
R3042 |
T6895 |
T6896 |
advmod |
indeed,patterns |
R3043 |
T6896 |
T6890 |
conj |
patterns,methods |
R3044 |
T6897 |
T6896 |
amod |
different,patterns |
R3045 |
T6898 |
T6896 |
compound |
expression,patterns |
R3046 |
T6899 |
T6896 |
prep |
in,patterns |
R3047 |
T6900 |
T6899 |
pobj |
mouse,in |
R3048 |
T6901 |
T6900 |
cc |
and,mouse |
R3049 |
T6902 |
T6900 |
conj |
human,mouse |
R3050 |
T6903 |
T6883 |
punct |
", ",repeated |
R3051 |
T6904 |
T6883 |
nsubj |
we,repeated |
R3052 |
T6905 |
T6906 |
det |
the,immunofluorescence |
R3053 |
T6906 |
T6883 |
dobj |
immunofluorescence,repeated |
R3054 |
T6907 |
T6906 |
prep |
of,immunofluorescence |
R3055 |
T6908 |
T6909 |
amod |
human,brain |
R3056 |
T6909 |
T6907 |
pobj |
brain,of |
R3057 |
T6910 |
T6883 |
punct |
.,repeated |
R3058 |
T6912 |
T6913 |
det |
This,time |
R3059 |
T6913 |
T6914 |
npadvmod |
time,investigated |
R3060 |
T6915 |
T6914 |
nsubj |
we,investigated |
R3061 |
T6916 |
T6914 |
dobj |
sections,investigated |
R3062 |
T6917 |
T6916 |
prep |
from,sections |
R3063 |
T6918 |
T6919 |
amod |
human,cortex |
R3064 |
T6919 |
T6917 |
pobj |
cortex,from |
R3065 |
T6920 |
T6919 |
amod |
parietal,cortex |
R3066 |
T6921 |
T6919 |
prep |
of,cortex |
R3067 |
T6922 |
T6923 |
compound |
autopsy,brain |
R3068 |
T6923 |
T6921 |
pobj |
brain,of |
R3069 |
T6924 |
T6914 |
punct |
.,investigated |
R3070 |
T6926 |
T6927 |
det |
The,expression |
R3071 |
T6927 |
T6929 |
nsubjpass |
expression,confirmed |
R3072 |
T6928 |
T6927 |
amod |
astroglial,expression |
R3073 |
T6930 |
T6927 |
prep |
of,expression |
R3074 |
T6931 |
T6932 |
compound |
Annexin,A7 |
R3075 |
T6932 |
T6930 |
pobj |
A7,of |
R3076 |
T6933 |
T6929 |
aux |
could,confirmed |
R3077 |
T6934 |
T6929 |
auxpass |
be,confirmed |
R3078 |
T6935 |
T6929 |
punct |
", ",confirmed |
R3079 |
T6936 |
T6937 |
mark |
although,exhibited |
R3080 |
T6937 |
T6929 |
advcl |
exhibited,confirmed |
R3081 |
T6938 |
T6939 |
neg |
not,astrocytes |
R3082 |
T6939 |
T6937 |
nsubj |
astrocytes,exhibited |
R3083 |
T6940 |
T6939 |
det |
all,astrocytes |
R3084 |
T6941 |
T6942 |
det |
the,staining |
R3085 |
T6942 |
T6937 |
dobj |
staining,exhibited |
R3086 |
T6943 |
T6942 |
amod |
nuclear,staining |
R3087 |
T6944 |
T6929 |
punct |
.,confirmed |
R3088 |
T6946 |
T6947 |
amod |
Pyramidal,neurons |
R3089 |
T6947 |
T6948 |
nsubj |
neurons,indicated |
R3090 |
T6949 |
T6948 |
advmod |
however,indicated |
R3091 |
T6950 |
T6951 |
det |
a,staining |
R3092 |
T6951 |
T6948 |
dobj |
staining,indicated |
R3093 |
T6952 |
T6951 |
amod |
distinct,staining |
R3094 |
T6953 |
T6951 |
prep |
of,staining |
R3095 |
T6954 |
T6955 |
compound |
Annexin,A7 |
R3096 |
T6955 |
T6953 |
pobj |
A7,of |
R3097 |
T6956 |
T6957 |
advmod |
most,prominent |
R3098 |
T6957 |
T6951 |
relcl |
prominent,staining |
R3099 |
T6958 |
T6957 |
prep |
along,prominent |
R3100 |
T6959 |
T6960 |
poss |
their,dendrites |
R3101 |
T6960 |
T6958 |
pobj |
dendrites,along |
R3102 |
T6961 |
T6948 |
punct |
.,indicated |
R3103 |
T6963 |
T6964 |
det |
The,results |
R3104 |
T6964 |
T6966 |
nsubjpass |
results,explained |
R3105 |
T6965 |
T6964 |
amod |
different,results |
R3106 |
T6967 |
T6964 |
acl |
obtained,results |
R3107 |
T6968 |
T6967 |
prep |
for,obtained |
R3108 |
T6969 |
T6970 |
det |
the,neurons |
R3109 |
T6970 |
T6968 |
pobj |
neurons,for |
R3110 |
T6971 |
T6966 |
aux |
may,explained |
R3111 |
T6972 |
T6966 |
auxpass |
be,explained |
R3112 |
T6973 |
T6966 |
agent |
by,explained |
R3113 |
T6974 |
T6975 |
det |
the,fact |
R3114 |
T6975 |
T6973 |
pobj |
fact,by |
R3115 |
T6976 |
T6977 |
mark |
that,used |
R3116 |
T6977 |
T6975 |
advcl |
used,fact |
R3117 |
T6978 |
T6977 |
nsubj |
we,used |
R3118 |
T6979 |
T6977 |
advmod |
previously,used |
R3119 |
T6980 |
T6981 |
amod |
frozen,sections |
R3120 |
T6981 |
T6977 |
dobj |
sections,used |
R3121 |
T6982 |
T6981 |
compound |
tissue,sections |
R3122 |
T6983 |
T6977 |
cc |
or,used |
R3123 |
T6984 |
T6985 |
mark |
that,derived |
R3124 |
T6985 |
T6977 |
conj |
derived,used |
R3125 |
T6986 |
T6985 |
nsubjpass |
they,derived |
R3126 |
T6987 |
T6985 |
auxpass |
were,derived |
R3127 |
T6988 |
T6985 |
prep |
from,derived |
R3128 |
T6989 |
T6988 |
pobj |
patients,from |
R3129 |
T6990 |
T6989 |
acl |
suffering,patients |
R3130 |
T6991 |
T6990 |
prep |
from,suffering |
R3131 |
T6992 |
T6993 |
amod |
temporal,lobe |
R3132 |
T6993 |
T6994 |
compound |
lobe,epilepsia |
R3133 |
T6994 |
T6991 |
pobj |
epilepsia,from |
R3134 |
T6995 |
T6966 |
punct |
.,explained |
R3135 |
T6997 |
T6998 |
prep |
On,was |
R3136 |
T6999 |
T7000 |
det |
the,hand |
R3137 |
T7000 |
T6997 |
pobj |
hand,On |
R3138 |
T7001 |
T7000 |
amod |
other,hand |
R3139 |
T7002 |
T7003 |
det |
the,tissue |
R3140 |
T7003 |
T6998 |
nsubj |
tissue,was |
R3141 |
T7004 |
T7003 |
amod |
actual,tissue |
R3142 |
T7005 |
T7003 |
amod |
human,tissue |
R3143 |
T7006 |
T7003 |
compound |
brain,tissue |
R3144 |
T7007 |
T7003 |
acl |
used,tissue |
R3145 |
T7008 |
T6998 |
prep |
from,was |
R3146 |
T7009 |
T7010 |
amod |
aged,patients |
R3147 |
T7010 |
T7008 |
pobj |
patients,from |
R3148 |
T7011 |
T6998 |
cc |
and,was |
R3149 |
T7012 |
T7013 |
aux |
did,correspond |
R3150 |
T7013 |
T6998 |
conj |
correspond,was |
R3151 |
T7014 |
T7013 |
neg |
not,correspond |
R3152 |
T7015 |
T7013 |
prep |
to,correspond |
R3153 |
T7016 |
T7017 |
det |
the,age |
R3154 |
T7017 |
T7015 |
pobj |
age,to |
R3155 |
T7018 |
T7017 |
prep |
of,age |
R3156 |
T7019 |
T7020 |
det |
the,mice |
R3157 |
T7020 |
T7018 |
pobj |
mice,of |
R3158 |
T7021 |
T7020 |
acl |
included,mice |
R3159 |
T7022 |
T7021 |
prep |
in,included |
R3160 |
T7023 |
T7024 |
det |
this,study |
R3161 |
T7024 |
T7022 |
pobj |
study,in |
R3162 |
T7025 |
T6998 |
punct |
.,was |
R3163 |
T7027 |
T7028 |
prep |
In,translocated |
R3164 |
T7029 |
T7030 |
amod |
cultured,cells |
R3165 |
T7030 |
T7027 |
pobj |
cells,In |
R3166 |
T7031 |
T7028 |
prep |
after,translocated |
R3167 |
T7032 |
T7033 |
compound |
cell,damage |
R3168 |
T7033 |
T7031 |
pobj |
damage,after |
R3169 |
T7034 |
T7033 |
cc |
or,damage |
R3170 |
T7035 |
T7033 |
conj |
apoptosis,damage |
R3171 |
T7036 |
T7037 |
punct |
(,observations |
R3172 |
T7037 |
T7035 |
meta |
observations,apoptosis |
R3173 |
T7038 |
T7037 |
amod |
unpublished,observations |
R3174 |
T7039 |
T7037 |
punct |
),observations |
R3175 |
T7040 |
T7031 |
cc |
or,after |
R3176 |
T7041 |
T7031 |
conj |
in,after |
R3177 |
T7042 |
T7041 |
pobj |
cells,in |
R3178 |
T7043 |
T7042 |
acl |
treated,cells |
R3179 |
T7044 |
T7043 |
prep |
with,treated |
R3180 |
T7045 |
T7046 |
det |
a,ionophore |
R3181 |
T7046 |
T7044 |
pobj |
ionophore,with |
R3182 |
T7047 |
T7046 |
compound |
Ca2+,ionophore |
R3183 |
T7048 |
T7046 |
punct |
-,ionophore |
R3184 |
T7049 |
T7050 |
compound |
Annexin,A7 |
R3185 |
T7050 |
T7028 |
nsubj |
A7,translocated |
R3186 |
T7051 |
T7028 |
prep |
from,translocated |
R3187 |
T7052 |
T7053 |
det |
the,cytoplasm |
R3188 |
T7053 |
T7051 |
pobj |
cytoplasm,from |
R3189 |
T7054 |
T7028 |
prep |
to,translocated |
R3190 |
T7055 |
T7056 |
amod |
cellular,membranes |
R3191 |
T7056 |
T7054 |
pobj |
membranes,to |
R3192 |
T7057 |
T7058 |
punct |
[,19 |
R3193 |
T7058 |
T7028 |
parataxis |
19,translocated |
R3194 |
T7059 |
T7058 |
punct |
],19 |
R3195 |
T7060 |
T7028 |
punct |
.,translocated |
R3196 |
T7062 |
T7063 |
nsubj |
We,favor |
R3197 |
T7064 |
T7063 |
advmod |
therefore,favor |
R3198 |
T7065 |
T7066 |
det |
the,hypothesis |
R3199 |
T7066 |
T7063 |
dobj |
hypothesis,favor |
R3200 |
T7067 |
T7068 |
mark |
that,translocated |
R3201 |
T7068 |
T7066 |
acl |
translocated,hypothesis |
R3202 |
T7069 |
T7070 |
compound |
Annexin,A7 |
R3203 |
T7070 |
T7068 |
nsubj |
A7,translocated |
R3204 |
T7071 |
T7070 |
prep |
in,A7 |
R3205 |
T7072 |
T7073 |
det |
the,neurons |
R3206 |
T7073 |
T7071 |
pobj |
neurons,in |
R3207 |
T7074 |
T7073 |
amod |
sensitive,neurons |
R3208 |
T7075 |
T7073 |
prep |
of,neurons |
R3209 |
T7076 |
T7077 |
det |
the,brain |
R3210 |
T7077 |
T7075 |
pobj |
brain,of |
R3211 |
T7078 |
T7077 |
amod |
human,brain |
R3212 |
T7079 |
T7077 |
compound |
autopsy,brain |
R3213 |
T7080 |
T7068 |
aux |
may,translocated |
R3214 |
T7081 |
T7068 |
aux |
have,translocated |
R3215 |
T7082 |
T7068 |
advmod |
similarly,translocated |
R3216 |
T7083 |
T7068 |
prep |
to,translocated |
R3217 |
T7084 |
T7085 |
det |
the,membrane |
R3218 |
T7085 |
T7083 |
pobj |
membrane,to |
R3219 |
T7086 |
T7085 |
amod |
cellular,membrane |
R3220 |
T7087 |
T7063 |
punct |
.,favor |
R3221 |
T7089 |
T7090 |
det |
This,property |
R3222 |
T7090 |
T7091 |
nsubj |
property,is |
R3223 |
T7092 |
T7090 |
prep |
of,property |
R3224 |
T7093 |
T7092 |
pobj |
translocation,of |
R3225 |
T7094 |
T7093 |
cc |
and,translocation |
R3226 |
T7095 |
T7096 |
compound |
membrane,binding |
R3227 |
T7096 |
T7093 |
conj |
binding,translocation |
R3228 |
T7097 |
T7091 |
acomp |
common,is |
R3229 |
T7098 |
T7097 |
prep |
to,common |
R3230 |
T7099 |
T7100 |
det |
all,annexins |
R3231 |
T7100 |
T7098 |
pobj |
annexins,to |
R3232 |
T7101 |
T7091 |
cc |
and,is |
R3233 |
T7102 |
T7103 |
advmod |
commercially,available |
R3234 |
T7103 |
T7104 |
amod |
available,kits |
R3235 |
T7104 |
T7105 |
nsubj |
kits,employ |
R3236 |
T7105 |
T7091 |
conj |
employ,is |
R3237 |
T7106 |
T7104 |
prep |
for,kits |
R3238 |
T7107 |
T7108 |
compound |
apoptosis,detection |
R3239 |
T7108 |
T7106 |
pobj |
detection,for |
R3240 |
T7109 |
T7110 |
amod |
recombinant,AnnexinA5 |
R3241 |
T7110 |
T7105 |
dobj |
AnnexinA5,employ |
R3242 |
T7111 |
T7105 |
punct |
.,employ |
R3243 |
T7113 |
T7114 |
det |
The,presence |
R3244 |
T7114 |
T7115 |
nsubjpass |
presence,confirmed |
R3245 |
T7116 |
T7114 |
prep |
of,presence |
R3246 |
T7117 |
T7118 |
amod |
nuclear,A7 |
R3247 |
T7118 |
T7116 |
pobj |
A7,of |
R3248 |
T7119 |
T7118 |
compound |
Annexin,A7 |
R3249 |
T7120 |
T7114 |
prep |
in,presence |
R3250 |
T7121 |
T7122 |
amod |
murine,brain |
R3251 |
T7122 |
T7120 |
pobj |
brain,in |
R3252 |
T7123 |
T7115 |
auxpass |
was,confirmed |
R3253 |
T7124 |
T7115 |
prep |
by,confirmed |
R3254 |
T7125 |
T7124 |
pobj |
controls,by |
R3255 |
T7126 |
T7125 |
acl |
using,controls |
R3256 |
T7127 |
T7126 |
dobj |
sections,using |
R3257 |
T7128 |
T7127 |
prep |
from,sections |
R3258 |
T7129 |
T7130 |
det |
the,mouse |
R3259 |
T7130 |
T7128 |
pobj |
mouse,from |
R3260 |
T7131 |
T7130 |
nmod |
AnxA7,mouse |
R3261 |
T7132 |
T7131 |
punct |
-,AnxA7 |
R3262 |
T7133 |
T7131 |
punct |
/,AnxA7 |
R3263 |
T7134 |
T7131 |
punct |
-,AnxA7 |
R3264 |
T7135 |
T7124 |
cc |
and,by |
R3265 |
T7136 |
T7124 |
conj |
by,by |
R3266 |
T7137 |
T7138 |
det |
a,extraction |
R3267 |
T7138 |
T7136 |
pobj |
extraction,by |
R3268 |
T7139 |
T7138 |
amod |
biochemical,extraction |
R3269 |
T7140 |
T7138 |
prep |
of,extraction |
R3270 |
T7141 |
T7142 |
det |
the,protein |
R3271 |
T7142 |
T7140 |
pobj |
protein,of |
R3272 |
T7143 |
T7138 |
prep |
from,extraction |
R3273 |
T7144 |
T7145 |
det |
the,nucleus |
R3274 |
T7145 |
T7143 |
pobj |
nucleus,from |
R3275 |
T7146 |
T7115 |
punct |
.,confirmed |
R3276 |
T7148 |
T7149 |
det |
Both,isoforms |
R3277 |
T7149 |
T7152 |
nsubjpass |
isoforms,expressed |
R3278 |
T7150 |
T7151 |
compound |
Annexin,A7 |
R3279 |
T7151 |
T7149 |
compound |
A7,isoforms |
R3280 |
T7153 |
T7149 |
acl |
described,isoforms |
R3281 |
T7154 |
T7153 |
prep |
in,described |
R3282 |
T7155 |
T7156 |
compound |
brain,tissue |
R3283 |
T7156 |
T7154 |
pobj |
tissue,in |
R3284 |
T7157 |
T7152 |
advmod |
seemingly,expressed |
R3285 |
T7158 |
T7152 |
auxpass |
are,expressed |
R3286 |
T7159 |
T7152 |
agent |
by,expressed |
R3287 |
T7160 |
T7159 |
pobj |
neurons,by |
R3288 |
T7161 |
T7160 |
cc |
and,neurons |
R3289 |
T7162 |
T7160 |
conj |
astrocytes,neurons |
R3290 |
T7163 |
T7152 |
punct |
", ",expressed |
R3291 |
T7164 |
T7165 |
dep |
which,shown |
R3292 |
T7165 |
T7152 |
advcl |
shown,expressed |
R3293 |
T7166 |
T7165 |
auxpass |
was,shown |
R3294 |
T7167 |
T7165 |
advcl |
using,shown |
R3295 |
T7168 |
T7169 |
amod |
total,extracts |
R3296 |
T7169 |
T7167 |
dobj |
extracts,using |
R3297 |
T7170 |
T7169 |
compound |
cell,extracts |
R3298 |
T7171 |
T7169 |
prep |
of,extracts |
R3299 |
T7172 |
T7173 |
amod |
cultured,cells |
R3300 |
T7173 |
T7171 |
pobj |
cells,of |
R3301 |
T7174 |
T7173 |
nmod |
neuo,cells |
R3302 |
T7175 |
T7174 |
punct |
-,neuo |
R3303 |
T7176 |
T7174 |
nummod |
2a,neuo |
R3304 |
T7177 |
T7174 |
punct |
", ",neuo |
R3305 |
T7178 |
T7174 |
conj |
PC,neuo |
R3306 |
T7179 |
T7178 |
punct |
-,PC |
R3307 |
T7180 |
T7178 |
nummod |
12,PC |
R3308 |
T7181 |
T7178 |
punct |
", ",PC |
R3309 |
T7182 |
T7178 |
cc |
and,PC |
R3310 |
T7183 |
T7178 |
conj |
C6,PC |
R3311 |
T7184 |
T7152 |
punct |
.,expressed |
R3312 |
T7186 |
T7187 |
prep |
In,described |
R3313 |
T7188 |
T7186 |
pobj |
addition,In |
R3314 |
T7189 |
T7188 |
prep |
to,addition |
R3315 |
T7190 |
T7191 |
poss |
their,expression |
R3316 |
T7191 |
T7189 |
pobj |
expression,to |
R3317 |
T7192 |
T7191 |
prep |
in,expression |
R3318 |
T7193 |
T7192 |
pobj |
brain,in |
R3319 |
T7194 |
T7187 |
punct |
", ",described |
R3320 |
T7195 |
T7196 |
preconj |
both,isoforms |
R3321 |
T7196 |
T7187 |
nsubjpass |
isoforms,described |
R3322 |
T7197 |
T7198 |
compound |
Annexin,A7 |
R3323 |
T7198 |
T7196 |
compound |
A7,isoforms |
R3324 |
T7199 |
T7187 |
aux |
have,described |
R3325 |
T7200 |
T7187 |
advmod |
only,described |
R3326 |
T7201 |
T7187 |
auxpass |
been,described |
R3327 |
T7202 |
T7187 |
prep |
in,described |
R3328 |
T7203 |
T7204 |
compound |
heart,muscle |
R3329 |
T7204 |
T7202 |
pobj |
muscle,in |
R3330 |
T7205 |
T7204 |
cc |
and,muscle |
R3331 |
T7206 |
T7207 |
amod |
red,cells |
R3332 |
T7207 |
T7204 |
conj |
cells,muscle |
R3333 |
T7208 |
T7207 |
compound |
blood,cells |
R3334 |
T7209 |
T7210 |
punct |
[,5 |
R3335 |
T7210 |
T7187 |
parataxis |
5,described |
R3336 |
T7211 |
T7212 |
punct |
-,8 |
R3337 |
T7212 |
T7210 |
prep |
8,5 |
R3338 |
T7213 |
T7210 |
punct |
],5 |
R3339 |
T7214 |
T7187 |
punct |
.,described |
R3340 |
T7216 |
T7217 |
mark |
Although,interfere |
R3341 |
T7217 |
T7227 |
advcl |
interfere,allowed |
R3342 |
T7218 |
T7219 |
det |
the,inactivation |
R3343 |
T7219 |
T7217 |
nsubj |
inactivation,interfere |
R3344 |
T7220 |
T7219 |
prep |
of,inactivation |
R3345 |
T7221 |
T7222 |
det |
the,gene |
R3346 |
T7222 |
T7220 |
pobj |
gene,of |
R3347 |
T7223 |
T7224 |
compound |
annexin,A7 |
R3348 |
T7224 |
T7222 |
compound |
A7,gene |
R3349 |
T7225 |
T7217 |
aux |
did,interfere |
R3350 |
T7226 |
T7217 |
neg |
not,interfere |
R3351 |
T7228 |
T7217 |
prep |
with,interfere |
R3352 |
T7229 |
T7230 |
det |
the,viability |
R3353 |
T7230 |
T7228 |
pobj |
viability,with |
R3354 |
T7231 |
T7230 |
cc |
and,viability |
R3355 |
T7232 |
T7230 |
conj |
development,viability |
R3356 |
T7233 |
T7230 |
prep |
of,viability |
R3357 |
T7234 |
T7235 |
amod |
knock,mice |
R3358 |
T7235 |
T7233 |
pobj |
mice,of |
R3359 |
T7236 |
T7234 |
prt |
out,knock |
R3360 |
T7237 |
T7238 |
punct |
[,10 |
R3361 |
T7238 |
T7217 |
parataxis |
10,interfere |
R3362 |
T7239 |
T7238 |
punct |
],10 |
R3363 |
T7240 |
T7227 |
punct |
", ",allowed |
R3364 |
T7241 |
T7242 |
poss |
their,generation |
R3365 |
T7242 |
T7227 |
nsubj |
generation,allowed |
R3366 |
T7243 |
T7244 |
nsubj |
us,address |
R3367 |
T7244 |
T7227 |
ccomp |
address,allowed |
R3368 |
T7245 |
T7244 |
aux |
to,address |
R3369 |
T7352 |
T7335 |
punct |
.,play |
R3370 |
T7354 |
T7355 |
nsubj |
One,speculate |
R3371 |
T7356 |
T7355 |
aux |
can,speculate |
R3372 |
T7357 |
T7355 |
prep |
about,speculate |
R3373 |
T7358 |
T7359 |
det |
an,involvement |
R3374 |
T7359 |
T7357 |
pobj |
involvement,about |
R3375 |
T7360 |
T7359 |
prep |
of,involvement |
R3376 |
T7361 |
T7362 |
compound |
Annexin,A7 |
R3377 |
T7246 |
T7247 |
det |
the,role |
R3378 |
T7362 |
T7360 |
pobj |
A7,of |
R3379 |
T7247 |
T7244 |
dobj |
role,address |
R3380 |
T7363 |
T7359 |
prep |
in,involvement |
R3381 |
T7248 |
T7247 |
prep |
of,role |
R3382 |
T7249 |
T7250 |
compound |
Annexin,A7 |
R3383 |
T7364 |
T7365 |
det |
the,regulation |
R3384 |
T7250 |
T7248 |
pobj |
A7,of |
R3385 |
T7251 |
T7247 |
prep |
in,role |
R3386 |
T7252 |
T7253 |
amod |
specific,types |
R3387 |
T7365 |
T7363 |
pobj |
regulation,in |
R3388 |
T7253 |
T7251 |
pobj |
types,in |
R3389 |
T7254 |
T7253 |
compound |
cell,types |
R3390 |
T7255 |
T7256 |
punct |
[,9 |
R3391 |
T7366 |
T7365 |
prep |
of,regulation |
R3392 |
T7256 |
T7227 |
parataxis |
9,allowed |
R3393 |
T7257 |
T7256 |
nummod |
8,9 |
R3394 |
T7258 |
T7256 |
punct |
",",9 |
R3395 |
T7367 |
T7368 |
det |
these,processes |
R3396 |
T7259 |
T7256 |
punct |
],9 |
R3397 |
T7260 |
T7227 |
punct |
.,allowed |
R3398 |
T7368 |
T7366 |
pobj |
processes,of |
R3399 |
T7369 |
T7370 |
npadvmod |
Ca2+,dependent |
R3400 |
T7262 |
T7263 |
advmod |
Indeed,found |
R3401 |
T7264 |
T7263 |
punct |
", ",found |
R3402 |
T7265 |
T7266 |
advmod |
when,analyzed |
R3403 |
T7266 |
T7263 |
advcl |
analyzed,found |
R3404 |
T7267 |
T7266 |
nsubj |
we,analyzed |
R3405 |
T7370 |
T7368 |
amod |
dependent,processes |
R3406 |
T7268 |
T7269 |
amod |
primary,astrocytes |
R3407 |
T7269 |
T7266 |
dobj |
astrocytes,analyzed |
R3408 |
T7270 |
T7266 |
prep |
from,analyzed |
R3409 |
T7371 |
T7370 |
punct |
-,dependent |
R3410 |
T7271 |
T7272 |
det |
an,mouse |
R3411 |
T7272 |
T7270 |
pobj |
mouse,from |
R3412 |
T7273 |
T7272 |
nmod |
AnxA7,mouse |
R3413 |
T7372 |
T7359 |
punct |
", ",involvement |
R3414 |
T7274 |
T7273 |
punct |
-,AnxA7 |
R3415 |
T7275 |
T7273 |
punct |
/,AnxA7 |
R3416 |
T7373 |
T7359 |
appos |
propositions,involvement |
R3417 |
T7276 |
T7273 |
punct |
-,AnxA7 |
R3418 |
T7277 |
T7266 |
prep |
for,analyzed |
R3419 |
T7374 |
T7375 |
dep |
that,need |
R3420 |
T7278 |
T7279 |
npadvmod |
Ca2+,dependent |
R3421 |
T7279 |
T7281 |
amod |
dependent,processes |
R3422 |
T7280 |
T7279 |
punct |
-,dependent |
R3423 |
T7281 |
T7277 |
pobj |
processes,for |
R3424 |
T7375 |
T7373 |
relcl |
need,propositions |
R3425 |
T7282 |
T7281 |
compound |
signaling,processes |
R3426 |
T7283 |
T7263 |
punct |
", ",found |
R3427 |
T7284 |
T7263 |
nsubj |
we,found |
R3428 |
T7376 |
T7377 |
amod |
further,investigation |
R3429 |
T7285 |
T7286 |
mark |
that,exhibited |
R3430 |
T7286 |
T7263 |
ccomp |
exhibited,found |
R3431 |
T7287 |
T7286 |
nsubj |
they,exhibited |
R3432 |
T7377 |
T7375 |
dobj |
investigation,need |
R3433 |
T7288 |
T7289 |
det |
a,velocity |
R3434 |
T7378 |
T7355 |
punct |
.,speculate |
R3435 |
T7380 |
T7381 |
advmod |
However,confirmed |
R3436 |
T7289 |
T7286 |
dobj |
velocity,exhibited |
R3437 |
T7290 |
T7291 |
advmod |
significantly,increased |
R3438 |
T7291 |
T7289 |
amod |
increased,velocity |
R3439 |
T7292 |
T7289 |
prep |
of,velocity |
R3440 |
T7382 |
T7381 |
punct |
", ",confirmed |
R3441 |
T7293 |
T7294 |
advmod |
mechanically,induced |
R3442 |
T7294 |
T7295 |
amod |
induced,waves |
R3443 |
T7295 |
T7292 |
pobj |
waves,of |
R3444 |
T7296 |
T7295 |
amod |
astrocytic,waves |
R3445 |
T7383 |
T7384 |
amod |
such,roles |
R3446 |
T7297 |
T7295 |
compound |
Ca2+,waves |
R3447 |
T7298 |
T7295 |
punct |
-,waves |
R3448 |
T7384 |
T7381 |
nsubjpass |
roles,confirmed |
R3449 |
T7299 |
T7300 |
mark |
as,compared |
R3450 |
T7300 |
T7286 |
advcl |
compared,exhibited |
R3451 |
T7301 |
T7300 |
prep |
to,compared |
R3452 |
T7385 |
T7381 |
auxpass |
are,confirmed |
R3453 |
T7302 |
T7303 |
amod |
wild,type |
R3454 |
T7303 |
T7301 |
pobj |
type,to |
R3455 |
T7304 |
T7305 |
punct |
[,9 |
R3456 |
T7305 |
T7286 |
parataxis |
9,exhibited |
R3457 |
T7306 |
T7305 |
punct |
],9 |
R3458 |
T7386 |
T7381 |
prep |
for,confirmed |
R3459 |
T7307 |
T7263 |
punct |
.,found |
R3460 |
T7309 |
T7310 |
nsubj |
This,led |
R3461 |
T7387 |
T7388 |
compound |
heart,function |
R3462 |
T7311 |
T7310 |
dobj |
us,led |
R3463 |
T7312 |
T7313 |
aux |
to,propose |
R3464 |
T7388 |
T7386 |
pobj |
function,for |
R3465 |
T7313 |
T7310 |
xcomp |
propose,led |
R3466 |
T7314 |
T7313 |
punct |
", ",propose |
R3467 |
T7315 |
T7316 |
mark |
that,act |
R3468 |
T7389 |
T7381 |
agent |
by,confirmed |
R3469 |
T7316 |
T7313 |
ccomp |
act,propose |
R3470 |
T7317 |
T7318 |
compound |
Annexin,A7 |
R3471 |
T7318 |
T7316 |
nsubj |
A7,act |
R3472 |
T7390 |
T7389 |
pobj |
studies,by |
R3473 |
T7319 |
T7316 |
aux |
can,act |
R3474 |
T7320 |
T7316 |
prep |
as,act |
R3475 |
T7391 |
T7390 |
prep |
of,studies |
R3476 |
T7321 |
T7322 |
det |
a,buffer |
R3477 |
T7322 |
T7320 |
pobj |
buffer,as |
R3478 |
T7323 |
T7322 |
compound |
Ca2+,buffer |
R3479 |
T7392 |
T7391 |
pobj |
Schrickel,of |
R3480 |
T7324 |
T7322 |
punct |
-,buffer |
R3481 |
T7325 |
T7316 |
cc |
and,act |
R3482 |
T7326 |
T7327 |
auxpass |
is,involved |
R3483 |
T7393 |
T7394 |
advmod |
et,al. |
R3484 |
T7327 |
T7316 |
conj |
involved,act |
R3485 |
T7328 |
T7327 |
prep |
in,involved |
R3486 |
T7329 |
T7330 |
compound |
Ca2+,homeostasis |
R3487 |
T7394 |
T7392 |
advmod |
al.,Schrickel |
R3488 |
T7330 |
T7328 |
pobj |
homeostasis,in |
R3489 |
T7331 |
T7330 |
punct |
-,homeostasis |
R3490 |
T7332 |
T7310 |
punct |
.,led |
R3491 |
T7395 |
T7396 |
punct |
[,submitted |
R3492 |
T7334 |
T7335 |
prep |
In,play |
R3493 |
T7396 |
T7392 |
meta |
submitted,Schrickel |
R3494 |
T7336 |
T7334 |
pobj |
neurons,In |
R3495 |
T7337 |
T7338 |
compound |
Ca2+,ions |
R3496 |
T7397 |
T7396 |
punct |
],submitted |
R3497 |
T7338 |
T7335 |
nsubj |
ions,play |
R3498 |
T7339 |
T7340 |
amod |
major,roles |
R3499 |
T7340 |
T7335 |
dobj |
roles,play |
R3500 |
T7398 |
T7392 |
punct |
", ",Schrickel |
R3501 |
T7341 |
T7335 |
prep |
in,play |
R3502 |
T7342 |
T7343 |
amod |
various,processes |
R3503 |
T7343 |
T7341 |
pobj |
processes,in |
R3504 |
T7399 |
T7400 |
dep |
who,described |
R3505 |
T7344 |
T7343 |
amod |
physiological,processes |
R3506 |
T7345 |
T7344 |
cc |
and,physiological |
R3507 |
T7346 |
T7344 |
conj |
pathophysiological,physiological |
R3508 |
T7347 |
T7348 |
punct |
[,20 |
R3509 |
T7348 |
T7335 |
parataxis |
20,play |
R3510 |
T7349 |
T7350 |
punct |
-,25 |
R3511 |
T7400 |
T7392 |
relcl |
described,Schrickel |
R3512 |
T7350 |
T7348 |
prep |
25,20 |
R3513 |
T7351 |
T7348 |
punct |
],20 |
R3514 |
T7401 |
T7402 |
det |
an,involvement |
R3515 |
T7402 |
T7400 |
dobj |
involvement,described |
R3516 |
T7403 |
T7402 |
prep |
of,involvement |
R3517 |
T7404 |
T7405 |
compound |
Annexin,A7 |
R3518 |
T7405 |
T7403 |
pobj |
A7,of |
R3519 |
T7458 |
T7456 |
pobj |
nuclei,from |
R3520 |
T7406 |
T7402 |
prep |
in,involvement |
R3521 |
T7459 |
T7453 |
prep |
in,lost |
R3522 |
T7460 |
T7461 |
det |
the,cord |
R3523 |
T7461 |
T7459 |
pobj |
cord,in |
R3524 |
T7407 |
T7408 |
det |
the,maintenance |
R3525 |
T7462 |
T7461 |
amod |
adult,cord |
R3526 |
T7463 |
T7461 |
amod |
spinal,cord |
R3527 |
T7408 |
T7406 |
pobj |
maintenance,in |
R3528 |
T7409 |
T7408 |
prep |
of,maintenance |
R3529 |
T7464 |
T7465 |
punct |
[,26 |
R3530 |
T7465 |
T7438 |
parataxis |
26,exhibited |
R3531 |
T7410 |
T7411 |
det |
a,electrophysiology |
R3532 |
T7466 |
T7465 |
punct |
],26 |
R3533 |
T7411 |
T7409 |
pobj |
electrophysiology,of |
R3534 |
T7467 |
T7438 |
punct |
.,exhibited |
R3535 |
T7469 |
T7470 |
prep |
In,show |
R3536 |
T7412 |
T7411 |
amod |
regular,electrophysiology |
R3537 |
T7471 |
T7469 |
pobj |
contrast,In |
R3538 |
T7472 |
T7470 |
punct |
", ",show |
R3539 |
T7413 |
T7411 |
amod |
cardiac,electrophysiology |
R3540 |
T7473 |
T7474 |
poss |
our,studies |
R3541 |
T7474 |
T7470 |
nsubj |
studies,show |
R3542 |
T7414 |
T7411 |
cc |
and,electrophysiology |
R3543 |
T7475 |
T7476 |
det |
a,relocation |
R3544 |
T7476 |
T7470 |
dobj |
relocation,show |
R3545 |
T7477 |
T7476 |
prep |
of,relocation |
R3546 |
T7415 |
T7416 |
compound |
Ca2+,homeostasis |
R3547 |
T7478 |
T7479 |
compound |
Annexin,A7 |
R3548 |
T7479 |
T7477 |
pobj |
A7,of |
R3549 |
T7416 |
T7411 |
conj |
homeostasis,electrophysiology |
R3550 |
T7480 |
T7476 |
prep |
from,relocation |
R3551 |
T7481 |
T7482 |
det |
the,cytosol |
R3552 |
T7482 |
T7480 |
pobj |
cytosol,from |
R3553 |
T7417 |
T7416 |
punct |
-,homeostasis |
R3554 |
T7483 |
T7476 |
prep |
to,relocation |
R3555 |
T7484 |
T7485 |
det |
the,nucleus |
R3556 |
T7485 |
T7483 |
pobj |
nucleus,to |
R3557 |
T7486 |
T7470 |
prep |
in,show |
R3558 |
T7487 |
T7486 |
pobj |
cells,in |
R3559 |
T7488 |
T7487 |
prep |
of,cells |
R3560 |
T7418 |
T7381 |
punct |
.,confirmed |
R3561 |
T7489 |
T7490 |
det |
the,tissue |
R3562 |
T7490 |
T7488 |
pobj |
tissue,of |
R3563 |
T7491 |
T7490 |
amod |
embryonic,tissue |
R3564 |
T7420 |
T7421 |
det |
An,location |
R3565 |
T7492 |
T7490 |
amod |
neuronal,tissue |
R3566 |
T7493 |
T7470 |
punct |
.,show |
R3567 |
T7495 |
T7496 |
det |
The,distribution |
R3568 |
T7421 |
T7424 |
nsubjpass |
location,reported |
R3569 |
T7496 |
T7499 |
nsubjpass |
distribution,determined |
R3570 |
T7497 |
T7498 |
compound |
Annexin,A7 |
R3571 |
T7498 |
T7496 |
compound |
A7,distribution |
R3572 |
T7422 |
T7421 |
amod |
altered,location |
R3573 |
T7500 |
T7499 |
auxpass |
is,determined |
R3574 |
T7423 |
T7421 |
amod |
subcellular,location |
R3575 |
T7501 |
T7499 |
agent |
by,determined |
R3576 |
T7502 |
T7503 |
det |
a,variety |
R3577 |
T7503 |
T7501 |
pobj |
variety,by |
R3578 |
T7425 |
T7421 |
prep |
during,location |
R3579 |
T7504 |
T7503 |
prep |
of,variety |
R3580 |
T7505 |
T7504 |
pobj |
factors,of |
R3581 |
T7506 |
T7499 |
punct |
.,determined |
R3582 |
T7426 |
T7425 |
pobj |
embryogenesis,during |
R3583 |
T7508 |
T7509 |
det |
An,one |
R3584 |
T7509 |
T7511 |
nsubj |
one,appears |
R3585 |
T7510 |
T7509 |
amod |
important,one |
R3586 |
T7427 |
T7424 |
auxpass |
was,reported |
R3587 |
T7428 |
T7424 |
advmod |
also,reported |
R3588 |
T7512 |
T7513 |
aux |
to,be |
R3589 |
T7513 |
T7511 |
xcomp |
be,appears |
R3590 |
T7514 |
T7513 |
attr |
Ca2+,be |
R3591 |
T7515 |
T7514 |
punct |
", ",Ca2+ |
R3592 |
T7429 |
T7424 |
prep |
for,reported |
R3593 |
T7516 |
T7517 |
dep |
which,promotes |
R3594 |
T7517 |
T7514 |
relcl |
promotes,Ca2+ |
R3595 |
T7518 |
T7517 |
dobj |
binding,promotes |
R3596 |
T7519 |
T7518 |
prep |
of,binding |
R3597 |
T7430 |
T7431 |
compound |
Annexin,A11 |
R3598 |
T7520 |
T7521 |
compound |
Annexin,A7 |
R3599 |
T7521 |
T7519 |
pobj |
A7,of |
R3600 |
T7522 |
T7518 |
prep |
to,binding |
R3601 |
T7431 |
T7429 |
pobj |
A11,for |
R3602 |
T7523 |
T7522 |
pobj |
membranes,to |
R3603 |
T7524 |
T7517 |
cc |
and,promotes |
R3604 |
T7525 |
T7526 |
advmod |
also,allows |
R3605 |
T7432 |
T7424 |
punct |
.,reported |
R3606 |
T7526 |
T7517 |
conj |
allows,promotes |
R3607 |
T7527 |
T7526 |
dobj |
aggregation,allows |
R3608 |
T7434 |
T7435 |
det |
The,matter |
R3609 |
T7528 |
T7527 |
prep |
of,aggregation |
R3610 |
T7529 |
T7528 |
pobj |
annexins,of |
R3611 |
T7530 |
T7531 |
punct |
[,27 |
R3612 |
T7531 |
T7513 |
parataxis |
27,be |
R3613 |
T7532 |
T7531 |
punct |
],27 |
R3614 |
T7533 |
T7511 |
punct |
.,appears |
R3615 |
T7535 |
T7536 |
compound |
Binding,partners |
R3616 |
T7435 |
T7438 |
nsubj |
matter,exhibited |
R3617 |
T7536 |
T7537 |
nsubj |
partners,represent |
R3618 |
T7538 |
T7536 |
prep |
of,partners |
R3619 |
T7436 |
T7435 |
amod |
developing,matter |
R3620 |
T7539 |
T7540 |
compound |
Annexin,A7 |
R3621 |
T7540 |
T7538 |
pobj |
A7,of |
R3622 |
T7541 |
T7542 |
amod |
such,as |
R3623 |
T7437 |
T7435 |
amod |
gray,matter |
R3624 |
T7542 |
T7536 |
prep |
as,partners |
R3625 |
T7543 |
T7542 |
pobj |
sorcin,as |
R3626 |
T7439 |
T7435 |
prep |
of,matter |
R3627 |
T7544 |
T7537 |
aux |
might,represent |
R3628 |
T7545 |
T7546 |
amod |
additional,factors |
R3629 |
T7546 |
T7537 |
dobj |
factors,represent |
R3630 |
T7547 |
T7548 |
punct |
[,28 |
R3631 |
T7548 |
T7537 |
parataxis |
28,represent |
R3632 |
T7549 |
T7548 |
punct |
],28 |
R3633 |
T7440 |
T7441 |
det |
the,cord |
R3634 |
T7550 |
T7537 |
punct |
.,represent |
R3635 |
T7441 |
T7439 |
pobj |
cord,of |
R3636 |
T7552 |
T7553 |
mark |
Although,found |
R3637 |
T7442 |
T7441 |
nmod |
rat,cord |
R3638 |
T7553 |
T7562 |
advcl |
found,described |
R3639 |
T7443 |
T7441 |
amod |
embryonic,cord |
R3640 |
T7554 |
T7555 |
det |
the,members |
R3641 |
T7555 |
T7553 |
nsubjpass |
members,found |
R3642 |
T7556 |
T7555 |
prep |
of,members |
R3643 |
T7444 |
T7441 |
amod |
spinal,cord |
R3644 |
T7557 |
T7558 |
det |
the,family |
R3645 |
T7558 |
T7556 |
pobj |
family,of |
R3646 |
T7559 |
T7558 |
compound |
annexin,family |
R3647 |
T7445 |
T7438 |
advmod |
primarily,exhibited |
R3648 |
T7560 |
T7553 |
auxpass |
are,found |
R3649 |
T7561 |
T7553 |
advmod |
generally,found |
R3650 |
T7446 |
T7447 |
amod |
nuclear,localization |
R3651 |
T7447 |
T7438 |
dobj |
localization,exhibited |
R3652 |
T7448 |
T7447 |
prep |
of,localization |
R3653 |
T7563 |
T7553 |
prep |
at,found |
R3654 |
T7449 |
T7450 |
compound |
Annexin,A11 |
R3655 |
T7450 |
T7448 |
pobj |
A11,of |
R3656 |
T7451 |
T7438 |
punct |
", ",exhibited |
R3657 |
T7452 |
T7453 |
mark |
while,lost |
R3658 |
T7564 |
T7565 |
det |
the,membrane |
R3659 |
T7565 |
T7563 |
pobj |
membrane,at |
R3660 |
T7453 |
T7438 |
advcl |
lost,exhibited |
R3661 |
T7566 |
T7565 |
compound |
plasma,membrane |
R3662 |
T7567 |
T7562 |
punct |
", ",described |
R3663 |
T7568 |
T7562 |
prep |
in,described |
R3664 |
T7569 |
T7570 |
det |
the,cytoplasm |
R3665 |
T7570 |
T7568 |
pobj |
cytoplasm,in |
R3666 |
T7454 |
T7453 |
nsubjpass |
immunoreactivity,lost |
R3667 |
T7571 |
T7568 |
cc |
or,in |
R3668 |
T7572 |
T7568 |
conj |
in,in |
R3669 |
T7573 |
T7572 |
pobj |
association,in |
R3670 |
T7574 |
T7573 |
prep |
with,association |
R3671 |
T7455 |
T7453 |
auxpass |
was,lost |
R3672 |
T7575 |
T7576 |
det |
the,cytoskeleton |
R3673 |
T7576 |
T7574 |
pobj |
cytoskeleton,with |
R3674 |
T7456 |
T7453 |
prep |
from,lost |
R3675 |
T7577 |
T7562 |
punct |
", ",described |
R3676 |
T7578 |
T7579 |
compound |
Annexins,A1 |
R3677 |
T7579 |
T7562 |
nsubjpass |
A1,described |
R3678 |
T7457 |
T7458 |
det |
the,nuclei |
R3679 |
T7580 |
T7579 |
punct |
", ",A1 |
R3680 |
T7581 |
T7579 |
conj |
A2,A1 |
R3681 |
T7582 |
T7581 |
punct |
", ",A2 |
R3682 |
T7670 |
T7671 |
det |
the,fibroblasts |
R3683 |
T7583 |
T7581 |
conj |
A4,A2 |
R3684 |
T7584 |
T7583 |
punct |
", ",A4 |
R3685 |
T7585 |
T7583 |
conj |
A5,A4 |
R3686 |
T7586 |
T7585 |
cc |
and,A5 |
R3687 |
T7587 |
T7585 |
conj |
A11,A5 |
R3688 |
T7671 |
T7669 |
pobj |
fibroblasts,of |
R3689 |
T7588 |
T7562 |
aux |
have,described |
R3690 |
T7589 |
T7562 |
auxpass |
been,described |
R3691 |
T7590 |
T7591 |
aux |
to,localized |
R3692 |
T7672 |
T7657 |
auxpass |
was,proposed |
R3693 |
T7591 |
T7562 |
xcomp |
localized,described |
R3694 |
T7592 |
T7591 |
auxpass |
be,localized |
R3695 |
T7593 |
T7594 |
advmod |
at,least |
R3696 |
T7673 |
T7657 |
punct |
.,proposed |
R3697 |
T7594 |
T7595 |
advmod |
least,partially |
R3698 |
T7595 |
T7591 |
advmod |
partially,localized |
R3699 |
T7596 |
T7591 |
prep |
in,localized |
R3700 |
T7597 |
T7598 |
det |
the,nucleus |
R3701 |
T7675 |
T7676 |
det |
These,results |
R3702 |
T7598 |
T7596 |
pobj |
nucleus,in |
R3703 |
T7599 |
T7600 |
punct |
[,30 |
R3704 |
T7600 |
T7562 |
parataxis |
30,described |
R3705 |
T7601 |
T7600 |
nummod |
26,30 |
R3706 |
T7676 |
T7677 |
nsubj |
results,mirror |
R3707 |
T7602 |
T7600 |
punct |
",",30 |
R3708 |
T7603 |
T7600 |
nummod |
29,30 |
R3709 |
T7604 |
T7600 |
punct |
",",30 |
R3710 |
T7678 |
T7677 |
dobj |
studies,mirror |
R3711 |
T7605 |
T7600 |
punct |
],30 |
R3712 |
T7606 |
T7562 |
punct |
.,described |
R3713 |
T7608 |
T7609 |
nsubj |
Studies,demonstrated |
R3714 |
T7610 |
T7608 |
prep |
with,Studies |
R3715 |
T7611 |
T7612 |
amod |
human,fibroblasts |
R3716 |
T7679 |
T7678 |
prep |
with,studies |
R3717 |
T7612 |
T7610 |
pobj |
fibroblasts,with |
R3718 |
T7613 |
T7612 |
compound |
foreskin,fibroblasts |
R3719 |
T7614 |
T7615 |
mark |
that,are |
R3720 |
T7615 |
T7609 |
ccomp |
are,demonstrated |
R3721 |
T7680 |
T7681 |
advmod |
stably,transfected |
R3722 |
T7616 |
T7617 |
compound |
Annexin,A1 |
R3723 |
T7617 |
T7615 |
nsubj |
A1,are |
R3724 |
T7618 |
T7617 |
punct |
", ",A1 |
R3725 |
T7681 |
T7682 |
amod |
transfected,cells |
R3726 |
T7619 |
T7617 |
conj |
A4,A1 |
R3727 |
T7620 |
T7619 |
cc |
and,A4 |
R3728 |
T7621 |
T7619 |
conj |
A5,A4 |
R3729 |
T7682 |
T7679 |
pobj |
cells,with |
R3730 |
T7622 |
T7615 |
advmod |
all,are |
R3731 |
T7623 |
T7615 |
acomp |
present,are |
R3732 |
T7683 |
T7682 |
compound |
C6,cells |
R3733 |
T7624 |
T7615 |
prep |
in,are |
R3734 |
T7625 |
T7626 |
det |
the,nucleus |
R3735 |
T7626 |
T7624 |
pobj |
nucleus,in |
R3736 |
T7684 |
T7678 |
punct |
", ",studies |
R3737 |
T7627 |
T7615 |
prep |
at,are |
R3738 |
T7628 |
T7629 |
amod |
higher,concentration |
R3739 |
T7629 |
T7627 |
pobj |
concentration,at |
R3740 |
T7630 |
T7629 |
prep |
than,concentration |
R3741 |
T7685 |
T7686 |
prep |
in,induced |
R3742 |
T7631 |
T7630 |
prep |
in,than |
R3743 |
T7632 |
T7633 |
det |
the,cytosol |
R3744 |
T7686 |
T7678 |
relcl |
induced,studies |
R3745 |
T7633 |
T7631 |
pobj |
cytosol,in |
R3746 |
T7634 |
T7635 |
punct |
[,31 |
R3747 |
T7635 |
T7615 |
parataxis |
31,are |
R3748 |
T7687 |
T7685 |
pobj |
which,in |
R3749 |
T7636 |
T7635 |
punct |
],31 |
R3750 |
T7637 |
T7609 |
punct |
.,demonstrated |
R3751 |
T7688 |
T7689 |
amod |
high,concentrations |
R3752 |
T7689 |
T7686 |
nsubj |
concentrations,induced |
R3753 |
T7639 |
T7640 |
csubj |
Raising,led |
R3754 |
T7690 |
T7689 |
amod |
intracellular,concentrations |
R3755 |
T7641 |
T7642 |
amod |
intracellular,Ca2+ |
R3756 |
T7691 |
T7689 |
compound |
Ca2+,concentrations |
R3757 |
T7642 |
T7639 |
dobj |
Ca2+,Raising |
R3758 |
T7643 |
T7640 |
prep |
to,led |
R3759 |
T7644 |
T7643 |
pobj |
relocation,to |
R3760 |
T7645 |
T7644 |
prep |
of,relocation |
R3761 |
T7692 |
T7689 |
punct |
-,concentrations |
R3762 |
T7646 |
T7647 |
det |
these,annexins |
R3763 |
T7647 |
T7645 |
pobj |
annexins,of |
R3764 |
T7693 |
T7694 |
det |
a,redistribution |
R3765 |
T7648 |
T7644 |
prep |
to,relocation |
R3766 |
T7649 |
T7650 |
det |
the,membrane |
R3767 |
T7650 |
T7648 |
pobj |
membrane,to |
R3768 |
T7651 |
T7650 |
amod |
nuclear,membrane |
R3769 |
T7652 |
T7640 |
punct |
.,led |
R3770 |
T7694 |
T7686 |
dobj |
redistribution,induced |
R3771 |
T7654 |
T7655 |
det |
An,role |
R3772 |
T7655 |
T7657 |
nsubjpass |
role,proposed |
R3773 |
T7695 |
T7694 |
amod |
marked,redistribution |
R3774 |
T7656 |
T7655 |
amod |
important,role |
R3775 |
T7658 |
T7655 |
prep |
for,role |
R3776 |
T7659 |
T7658 |
pobj |
annexins,for |
R3777 |
T7696 |
T7694 |
prep |
of,redistribution |
R3778 |
T7660 |
T7655 |
prep |
in,role |
R3779 |
T7661 |
T7660 |
pcomp |
mediating,in |
R3780 |
T7697 |
T7698 |
compound |
Annexin,A7 |
R3781 |
T7662 |
T7663 |
det |
the,signal |
R3782 |
T7663 |
T7661 |
dobj |
signal,mediating |
R3783 |
T7664 |
T7663 |
compound |
Ca2+,signal |
R3784 |
T7698 |
T7696 |
pobj |
A7,of |
R3785 |
T7665 |
T7663 |
punct |
-,signal |
R3786 |
T7666 |
T7661 |
prep |
within,mediating |
R3787 |
T7667 |
T7668 |
det |
the,nuclei |
R3788 |
T7699 |
T7694 |
prep |
from,redistribution |
R3789 |
T7668 |
T7666 |
pobj |
nuclei,within |
R3790 |
T7669 |
T7668 |
prep |
of,nuclei |
R3791 |
T7700 |
T7701 |
poss |
its,localization |
R3792 |
T7701 |
T7699 |
pobj |
localization,from |
R3793 |
T7702 |
T7701 |
prep |
in,localization |
R3794 |
T7776 |
T7777 |
punct |
[,32 |
R3795 |
T7777 |
T7775 |
parataxis |
32,Moss |
R3796 |
T7703 |
T7704 |
det |
the,nucleoplasm |
R3797 |
T7778 |
T7777 |
punct |
],32 |
R3798 |
T7779 |
T7772 |
punct |
", ",showed |
R3799 |
T7780 |
T7781 |
mark |
that,assemble |
R3800 |
T7704 |
T7702 |
pobj |
nucleoplasm,in |
R3801 |
T7781 |
T7772 |
ccomp |
assemble,showed |
R3802 |
T7782 |
T7783 |
compound |
Annexin,A11 |
R3803 |
T7705 |
T7694 |
prep |
to,redistribution |
R3804 |
T7783 |
T7781 |
nsubj |
A11,assemble |
R3805 |
T7784 |
T7783 |
cc |
and,A11 |
R3806 |
T7706 |
T7707 |
det |
the,membrane |
R3807 |
T7785 |
T7783 |
conj |
S100A6,A11 |
R3808 |
T7786 |
T7781 |
prep |
at,assemble |
R3809 |
T7787 |
T7788 |
det |
the,envelope |
R3810 |
T7788 |
T7786 |
pobj |
envelope,at |
R3811 |
T7789 |
T7788 |
amod |
nuclear,envelope |
R3812 |
T7707 |
T7705 |
pobj |
membrane,to |
R3813 |
T7790 |
T7781 |
prep |
during,assemble |
R3814 |
T7791 |
T7792 |
amod |
nuclear,breakdown |
R3815 |
T7792 |
T7790 |
pobj |
breakdown,during |
R3816 |
T7708 |
T7707 |
amod |
nuclear,membrane |
R3817 |
T7793 |
T7772 |
punct |
.,showed |
R3818 |
T7795 |
T7796 |
poss |
Their,role |
R3819 |
T7796 |
T7797 |
nsubjpass |
role,known |
R3820 |
T7798 |
T7796 |
prep |
in,role |
R3821 |
T7709 |
T7710 |
punct |
[,19 |
R3822 |
T7799 |
T7800 |
det |
this,process |
R3823 |
T7800 |
T7798 |
pobj |
process,in |
R3824 |
T7801 |
T7797 |
auxpass |
is,known |
R3825 |
T7802 |
T7797 |
neg |
not,known |
R3826 |
T7710 |
T7677 |
parataxis |
19,mirror |
R3827 |
T7803 |
T7797 |
punct |
.,known |
R3828 |
T7805 |
T7806 |
prep |
In,seems |
R3829 |
T7711 |
T7710 |
punct |
],19 |
R3830 |
T7807 |
T7805 |
amod |
general,In |
R3831 |
T7712 |
T7677 |
punct |
.,mirror |
R3832 |
T7808 |
T7806 |
nsubj |
it,seems |
R3833 |
T7809 |
T7810 |
mark |
that,regulated |
R3834 |
T7714 |
T7715 |
nsubj |
None,contains |
R3835 |
T7810 |
T7806 |
ccomp |
regulated,seems |
R3836 |
T7716 |
T7714 |
prep |
of,None |
R3837 |
T7811 |
T7812 |
det |
the,localization |
R3838 |
T7812 |
T7810 |
nsubjpass |
localization,regulated |
R3839 |
T7813 |
T7812 |
amod |
nuclear,localization |
R3840 |
T7814 |
T7812 |
prep |
of,localization |
R3841 |
T7717 |
T7718 |
det |
the,annexins |
R3842 |
T7815 |
T7816 |
det |
the,annexins |
R3843 |
T7816 |
T7814 |
pobj |
annexins,of |
R3844 |
T7817 |
T7810 |
auxpass |
is,regulated |
R3845 |
T7718 |
T7716 |
pobj |
annexins,of |
R3846 |
T7818 |
T7810 |
advmod |
actively,regulated |
R3847 |
T7819 |
T7806 |
punct |
.,seems |
R3848 |
T7719 |
T7720 |
det |
a,signal |
R3849 |
T7821 |
T7822 |
prep |
For,controlled |
R3850 |
T7823 |
T7821 |
pobj |
example,For |
R3851 |
T7720 |
T7715 |
dobj |
signal,contains |
R3852 |
T7824 |
T7825 |
det |
the,compartmentalization |
R3853 |
T7825 |
T7822 |
nsubjpass |
compartmentalization,controlled |
R3854 |
T7721 |
T7720 |
amod |
typical,signal |
R3855 |
T7826 |
T7825 |
amod |
nucleocytoplasmic,compartmentalization |
R3856 |
T7827 |
T7825 |
prep |
of,compartmentalization |
R3857 |
T7828 |
T7829 |
compound |
Annexin,A2 |
R3858 |
T7722 |
T7723 |
amod |
nuclear,localization |
R3859 |
T7829 |
T7827 |
pobj |
A2,of |
R3860 |
T7830 |
T7822 |
auxpass |
is,controlled |
R3861 |
T7831 |
T7822 |
agent |
by,controlled |
R3862 |
T7723 |
T7720 |
compound |
localization,signal |
R3863 |
T7832 |
T7831 |
pobj |
sequestration,by |
R3864 |
T7833 |
T7832 |
prep |
of,sequestration |
R3865 |
T7834 |
T7835 |
det |
the,complex |
R3866 |
T7724 |
T7715 |
cc |
and,contains |
R3867 |
T7835 |
T7833 |
pobj |
complex,of |
R3868 |
T7836 |
T7837 |
compound |
AnxA2,p11 |
R3869 |
T7837 |
T7835 |
compound |
p11,complex |
R3870 |
T7725 |
T7726 |
poss |
their,mechanism |
R3871 |
T7838 |
T7837 |
punct |
/,p11 |
R3872 |
T7839 |
T7835 |
acl |
modulated,complex |
R3873 |
T7840 |
T7839 |
prep |
by,modulated |
R3874 |
T7841 |
T7840 |
pobj |
phosphorylation,by |
R3875 |
T7842 |
T7840 |
cc |
and,by |
R3876 |
T7843 |
T7840 |
conj |
by,by |
R3877 |
T7726 |
T7727 |
nsubj |
mechanism,remains |
R3878 |
T7844 |
T7845 |
det |
a,signal |
R3879 |
T7845 |
T7843 |
pobj |
signal,by |
R3880 |
T7846 |
T7845 |
amod |
nuclear,signal |
R3881 |
T7727 |
T7715 |
conj |
remains,contains |
R3882 |
T7847 |
T7845 |
compound |
export,signal |
R3883 |
T7848 |
T7845 |
acl |
found,signal |
R3884 |
T7849 |
T7848 |
prep |
in,found |
R3885 |
T7728 |
T7726 |
prep |
of,mechanism |
R3886 |
T7850 |
T7851 |
det |
the,region |
R3887 |
T7851 |
T7849 |
pobj |
region,in |
R3888 |
T7852 |
T7853 |
nmod |
AnxA2,3 |
R3889 |
T7729 |
T7730 |
amod |
nuclear,import |
R3890 |
T7853 |
T7851 |
nummod |
3,region |
R3891 |
T7854 |
T7855 |
punct |
–,12 |
R3892 |
T7855 |
T7853 |
prep |
12,3 |
R3893 |
T7730 |
T7728 |
pobj |
import,of |
R3894 |
T7856 |
T7857 |
punct |
[,33 |
R3895 |
T7857 |
T7822 |
parataxis |
33,controlled |
R3896 |
T7731 |
T7732 |
aux |
to,elucidated |
R3897 |
T7858 |
T7857 |
punct |
],33 |
R3898 |
T7859 |
T7822 |
punct |
.,controlled |
R3899 |
T7732 |
T7727 |
xcomp |
elucidated,remains |
R3900 |
T7861 |
T7862 |
nummod |
One,function |
R3901 |
T7862 |
T7863 |
nsubjpass |
function,suggested |
R3902 |
T7733 |
T7732 |
auxpass |
be,elucidated |
R3903 |
T7864 |
T7862 |
prep |
of,function |
R3904 |
T7865 |
T7866 |
compound |
Annexin,A2 |
R3905 |
T7734 |
T7727 |
punct |
.,remains |
R3906 |
T7866 |
T7864 |
pobj |
A2,of |
R3907 |
T7867 |
T7862 |
prep |
in,function |
R3908 |
T7868 |
T7869 |
det |
the,nucleus |
R3909 |
T7736 |
T7737 |
prep |
For,shown |
R3910 |
T7869 |
T7867 |
pobj |
nucleus,in |
R3911 |
T7870 |
T7862 |
punct |
", ",function |
R3912 |
T7871 |
T7872 |
dep |
that,appears |
R3913 |
T7738 |
T7739 |
compound |
Annexin,A11 |
R3914 |
T7872 |
T7862 |
relcl |
appears,function |
R3915 |
T7873 |
T7874 |
neg |
not,involve |
R3916 |
T7874 |
T7872 |
xcomp |
involve,appears |
R3917 |
T7875 |
T7874 |
aux |
to,involve |
R3918 |
T7876 |
T7874 |
dobj |
binding,involve |
R3919 |
T7739 |
T7736 |
pobj |
A11,For |
R3920 |
T7877 |
T7876 |
prep |
of,binding |
R3921 |
T7878 |
T7877 |
pobj |
p11,of |
R3922 |
T7740 |
T7737 |
nsubjpass |
it,shown |
R3923 |
T7879 |
T7863 |
punct |
", ",suggested |
R3924 |
T7880 |
T7863 |
aux |
has,suggested |
R3925 |
T7881 |
T7863 |
auxpass |
been,suggested |
R3926 |
T7741 |
T7737 |
auxpass |
was,shown |
R3927 |
T7742 |
T7743 |
mark |
that,mediated |
R3928 |
T7743 |
T7737 |
ccomp |
mediated,shown |
R3929 |
T7744 |
T7745 |
amod |
nuclear,localization |
R3930 |
T7882 |
T7863 |
agent |
by,suggested |
R3931 |
T7745 |
T7743 |
nsubjpass |
localization,mediated |
R3932 |
T7883 |
T7884 |
poss |
its,purification |
R3933 |
T7884 |
T7882 |
pobj |
purification,by |
R3934 |
T7885 |
T7863 |
prep |
as,suggested |
R3935 |
T7886 |
T7885 |
pobj |
part,as |
R3936 |
T7746 |
T7743 |
auxpass |
is,mediated |
R3937 |
T7887 |
T7886 |
prep |
of,part |
R3938 |
T7888 |
T7889 |
det |
a,complex |
R3939 |
T7889 |
T7887 |
pobj |
complex,of |
R3940 |
T7747 |
T7743 |
agent |
by,mediated |
R3941 |
T7890 |
T7891 |
compound |
primer,recognition |
R3942 |
T7891 |
T7889 |
compound |
recognition,complex |
R3943 |
T7748 |
T7749 |
poss |
its,region |
R3944 |
T7892 |
T7889 |
compound |
protein,complex |
R3945 |
T7893 |
T7894 |
dep |
that,enhances |
R3946 |
T7894 |
T7889 |
relcl |
enhances,complex |
R3947 |
T7749 |
T7747 |
pobj |
region,by |
R3948 |
T7895 |
T7896 |
compound |
DNA,polymeraseα |
R3949 |
T7896 |
T7897 |
compound |
polymeraseα,activity |
R3950 |
T7897 |
T7894 |
dobj |
activity,enhances |
R3951 |
T7750 |
T7751 |
npadvmod |
N,terminal |
R3952 |
T7898 |
T7897 |
punct |
-,activity |
R3953 |
T7899 |
T7900 |
advmod |
in,vitro |
R3954 |
T7900 |
T7894 |
advmod |
vitro,enhances |
R3955 |
T7751 |
T7749 |
amod |
terminal,region |
R3956 |
T7752 |
T7751 |
punct |
-,terminal |
R3957 |
T7753 |
T7749 |
punct |
", ",region |
R3960 |
T7754 |
T7755 |
dep |
which,contains |
R3963 |
T7755 |
T7749 |
relcl |
contains,region |
R3966 |
T7756 |
T7755 |
advmod |
also,contains |
R3967 |
T7909 |
T7908 |
aux |
may,participate |
R3968 |
T7757 |
T7758 |
det |
a,side |
R3969 |
T7910 |
T7908 |
prep |
in,participate |
R3970 |
T7911 |
T7912 |
det |
a,response |
R3971 |
T7758 |
T7755 |
dobj |
side,contains |
R3972 |
T7912 |
T7910 |
pobj |
response,in |
R3973 |
T7913 |
T7912 |
amod |
nuclear,response |
R3974 |
T7759 |
T7758 |
compound |
binding,side |
R3975 |
T7914 |
T7912 |
prep |
to,response |
R3976 |
T7915 |
T7916 |
amod |
initial,stimulation |
R3977 |
T7916 |
T7914 |
pobj |
stimulation,to |
R3978 |
T7917 |
T7916 |
compound |
cell,stimulation |
R3979 |
T7918 |
T7914 |
cc |
or,to |
R3980 |
T7919 |
T7914 |
conj |
to,to |
R3981 |
T7760 |
T7758 |
prep |
for,side |
R3982 |
T7920 |
T7921 |
det |
a,Ca2+ |
R3983 |
T7921 |
T7919 |
pobj |
Ca2+,to |
R3984 |
T7922 |
T7921 |
punct |
-,Ca2+ |
R3985 |
T7761 |
T7762 |
det |
the,protein |
R3986 |
T7923 |
T7921 |
amod |
transient,Ca2+ |
R3987 |
T7924 |
T7908 |
punct |
", ",participate |
R3988 |
T7925 |
T7926 |
advmod |
presumably,by |
R3989 |
T7762 |
T7760 |
pobj |
protein,for |
R3990 |
T7926 |
T7908 |
prep |
by,participate |
R3991 |
T7763 |
T7762 |
compound |
S100,protein |
R3992 |
T7927 |
T7926 |
pcomp |
regulating,by |
R3993 |
T7928 |
T7929 |
compound |
DNA,replication |
R3994 |
T7929 |
T7927 |
dobj |
replication,regulating |
R3995 |
T7764 |
T7762 |
appos |
calcyclin,protein |
R3996 |
T7930 |
T7908 |
punct |
.,participate |
R3997 |
T7765 |
T7766 |
punct |
[,29 |
R3998 |
T7932 |
T7933 |
prep |
For,is |
R3999 |
T7934 |
T7935 |
compound |
Annexin,A7 |
R4000 |
T7766 |
T7737 |
parataxis |
29,shown |
R4001 |
T7935 |
T7932 |
pobj |
A7,For |
R4002 |
T7936 |
T7937 |
predet |
such,pathway |
R4003 |
T7937 |
T7933 |
nsubj |
pathway,is |
R4004 |
T7767 |
T7766 |
punct |
],29 |
R4005 |
T7938 |
T7937 |
det |
a,pathway |
R4006 |
T7939 |
T7940 |
advmod |
very,speculative |
R4007 |
T7940 |
T7933 |
acomp |
speculative,is |
R4008 |
T7768 |
T7737 |
punct |
.,shown |
R4009 |
T7941 |
T7933 |
prep |
at,is |
R4010 |
T7942 |
T7943 |
det |
the,moment |
R4011 |
T7770 |
T7771 |
advmod |
More,recently |
R4012 |
T7943 |
T7941 |
pobj |
moment,at |
R4013 |
T7944 |
T7933 |
punct |
.,is |
R4014 |
T7946 |
T7947 |
compound |
Future,studies |
R4015 |
T7947 |
T7948 |
nsubjpass |
studies,directed |
R4016 |
T7771 |
T7772 |
advmod |
recently,showed |
R4017 |
T7949 |
T7948 |
auxpass |
are,directed |
R4018 |
T7773 |
T7772 |
nsubj |
Tomas,showed |
R4019 |
T7950 |
T7948 |
advmod |
however,directed |
R4020 |
T7951 |
T7948 |
agent |
by,directed |
R4021 |
T7952 |
T7953 |
det |
these,findings |
R4022 |
T7953 |
T7951 |
pobj |
findings,by |
R4023 |
T7774 |
T7773 |
cc |
and,Tomas |
R4024 |
T7954 |
T7948 |
cc |
and,directed |
R4025 |
T7955 |
T7956 |
aux |
will,concentrate |
R4026 |
T7775 |
T7773 |
conj |
Moss,Tomas |
R4027 |
T7956 |
T7948 |
conj |
concentrate,directed |
R4028 |
T7957 |
T7956 |
prep |
on,concentrate |
R4029 |
T7958 |
T7959 |
det |
the,identification |
R4030 |
T7959 |
T7957 |
pobj |
identification,on |
R4031 |
T7960 |
T7959 |
prep |
of,identification |
R4032 |
T7961 |
T7962 |
det |
the,signal |
R4033 |
T7962 |
T7960 |
pobj |
signal,of |
R4034 |
T7963 |
T7962 |
amod |
nuclear,signal |
R4035 |
T7964 |
T7962 |
compound |
localization,signal |
R4036 |
T7965 |
T7962 |
prep |
of,signal |
R4037 |
T7966 |
T7967 |
compound |
Annexin,A7 |
R4038 |
T7967 |
T7965 |
pobj |
A7,of |
R4039 |
T7968 |
T7969 |
advmod |
as,as |
R4040 |
T7969 |
T7957 |
cc |
as,on |
R4041 |
T7970 |
T7969 |
advmod |
well,as |
R4042 |
T7971 |
T7957 |
conj |
on,on |
R4043 |
T7972 |
T7973 |
det |
the,role |
R4044 |
T7973 |
T7971 |
pobj |
role,on |
R4045 |
T7974 |
T7973 |
prep |
of,role |
R4046 |
T7975 |
T7976 |
compound |
Annexin,A7 |
R4047 |
T7976 |
T7974 |
pobj |
A7,of |
R4048 |
T7977 |
T7973 |
prep |
in,role |
R4049 |
T7978 |
T7979 |
det |
the,compartment |
R4050 |
T7979 |
T7977 |
pobj |
compartment,in |
R4051 |
T7980 |
T7979 |
amod |
nuclear,compartment |
R4052 |
T7981 |
T7948 |
punct |
.,directed |
R4059 |
T8198 |
T8199 |
prep |
In,report |
R4060 |
T8200 |
T8201 |
det |
this,article |
R4061 |
T8201 |
T8198 |
pobj |
article,In |
R4062 |
T8202 |
T8199 |
nsubj |
we,report |
R4063 |
T8203 |
T8204 |
det |
the,translocation |
R4064 |
T8204 |
T8199 |
dobj |
translocation,report |
R4065 |
T8205 |
T8204 |
prep |
of,translocation |
R4066 |
T8206 |
T8207 |
compound |
Annexin,A7 |
R4067 |
T8207 |
T8205 |
pobj |
A7,of |
R4068 |
T8208 |
T8204 |
prep |
to,translocation |
R4069 |
T8209 |
T8208 |
pobj |
nuclei,to |
R4070 |
T8210 |
T8209 |
prep |
of,nuclei |
R4071 |
T8211 |
T8212 |
amod |
differentiating,cells |
R4072 |
T8212 |
T8210 |
pobj |
cells,of |
R4073 |
T8213 |
T8204 |
prep |
in,translocation |
R4074 |
T8214 |
T8215 |
det |
the,brain |
R4075 |
T8215 |
T8213 |
pobj |
brain,in |
R4076 |
T8216 |
T8215 |
amod |
developing,brain |
R4077 |
T8217 |
T8215 |
amod |
murine,brain |
R4078 |
T8218 |
T8199 |
punct |
.,report |
R4079 |
T8220 |
T8221 |
prep |
In,detected |
R4080 |
T8222 |
T8223 |
det |
the,brain |
R4081 |
T8223 |
T8220 |
pobj |
brain,In |
R4082 |
T8224 |
T8223 |
amod |
adult,brain |
R4083 |
T8225 |
T8226 |
compound |
Annexin,A7 |
R4084 |
T8226 |
T8221 |
nsubjpass |
A7,detected |
R4085 |
T8227 |
T8221 |
advmod |
generally,detected |
R4086 |
T8228 |
T8221 |
auxpass |
was,detected |
R4087 |
T8229 |
T8221 |
prep |
in,detected |
R4088 |
T8230 |
T8229 |
pobj |
nuclei,in |
R4089 |
T8231 |
T8230 |
prep |
of,nuclei |
R4090 |
T8232 |
T8231 |
pobj |
neurons,of |
R4091 |
T8233 |
T8221 |
punct |
.,detected |
R4092 |
T8235 |
T8236 |
nsubj |
Astrocytes,exhibited |
R4093 |
T8237 |
T8235 |
punct |
", ",Astrocytes |
R4094 |
T8238 |
T8239 |
amod |
cerebellar,cells |
R4095 |
T8239 |
T8235 |
conj |
cells,Astrocytes |
R4096 |
T8240 |
T8239 |
compound |
Purkinje,cells |
R4097 |
T8241 |
T8239 |
punct |
-,cells |
R4098 |
T8242 |
T8239 |
cc |
and,cells |
R4099 |
T8243 |
T8244 |
amod |
neocortical,neurons |
R4100 |
T8244 |
T8239 |
conj |
neurons,cells |
R4101 |
T8245 |
T8244 |
amod |
pyramidal,neurons |
R4102 |
T8246 |
T8247 |
preconj |
both,A7 |
R4103 |
T8247 |
T8236 |
dobj |
A7,exhibited |
R4104 |
T8248 |
T8247 |
punct |
", ",A7 |
R4105 |
T8249 |
T8247 |
compound |
Annexin,A7 |
R4106 |
T8250 |
T8247 |
prep |
in,A7 |
R4107 |
T8251 |
T8252 |
det |
the,cytosol |
R4108 |
T8252 |
T8250 |
pobj |
cytosol,in |
R4109 |
T8253 |
T8250 |
cc |
and,in |
R4110 |
T8254 |
T8250 |
conj |
in,in |
R4111 |
T8255 |
T8256 |
det |
the,nuclei |
R4112 |
T8256 |
T8254 |
pobj |
nuclei,in |
R4113 |
T8257 |
T8236 |
punct |
.,exhibited |
R4114 |
T8259 |
T8260 |
advmod |
Thus,depends |
R4115 |
T8261 |
T8260 |
punct |
", ",depends |
R4116 |
T8262 |
T8263 |
det |
the,localisation |
R4117 |
T8263 |
T8260 |
nsubj |
localisation,depends |
R4118 |
T8264 |
T8263 |
amod |
subcellular,localisation |
R4119 |
T8265 |
T8263 |
prep |
of,localisation |
R4120 |
T8266 |
T8267 |
compound |
Annexin,A7 |
R4121 |
T8267 |
T8265 |
pobj |
A7,of |
R4122 |
T8268 |
T8260 |
prep |
on,depends |
R4123 |
T8269 |
T8270 |
det |
the,stage |
R4124 |
T8270 |
T8268 |
pobj |
stage,on |
R4125 |
T8271 |
T8270 |
amod |
developmental,stage |
R4126 |
T8272 |
T8270 |
cc |
and,stage |
R4127 |
T8273 |
T8274 |
det |
the,type |
R4128 |
T8274 |
T8270 |
conj |
type,stage |
R4129 |
T8275 |
T8274 |
compound |
cell,type |
R4130 |
T8276 |
T8260 |
punct |
.,depends |
R4131 |
T8278 |
T8279 |
det |
A,role |
R4132 |
T8279 |
T8280 |
nsubj |
role,remains |
R4133 |
T8281 |
T8279 |
prep |
of,role |
R4134 |
T8282 |
T8283 |
compound |
Annexin,A7 |
R4135 |
T8283 |
T8281 |
pobj |
A7,of |
R4136 |
T8284 |
T8285 |
advmod |
as,as |
R4137 |
T8285 |
T8281 |
cc |
as,of |
R4138 |
T8286 |
T8285 |
advmod |
well,as |
R4139 |
T8287 |
T8281 |
conj |
of,of |
R4140 |
T8288 |
T8289 |
amod |
other,annexins |
R4141 |
T8289 |
T8287 |
pobj |
annexins,of |
R4142 |
T8290 |
T8279 |
prep |
in,role |
R4143 |
T8291 |
T8290 |
pobj |
nuclei,in |
R4144 |
T8292 |
T8280 |
acomp |
speculative,remains |
R4145 |
T8293 |
T8280 |
punct |
.,remains |
R4146 |
T8295 |
T8296 |
det |
The,results |
R4147 |
T8296 |
T8297 |
nsubjpass |
results,confirmed |
R4148 |
T8298 |
T8296 |
acl |
obtained,results |
R4149 |
T8299 |
T8298 |
prep |
by,obtained |
R4150 |
T8300 |
T8299 |
pobj |
immunofluorescence,by |
R4151 |
T8301 |
T8297 |
auxpass |
were,confirmed |
R4152 |
T8302 |
T8297 |
prep |
by,confirmed |
R4153 |
T8303 |
T8302 |
pobj |
extraction,by |
R4154 |
T8304 |
T8303 |
prep |
of,extraction |
R4155 |
T8305 |
T8306 |
compound |
Annexin,A7 |
R4156 |
T8306 |
T8304 |
pobj |
A7,of |
R4157 |
T8307 |
T8303 |
prep |
from,extraction |
R4158 |
T8308 |
T8307 |
pobj |
nuclei,from |
R4159 |
T8309 |
T8308 |
prep |
of,nuclei |
R4160 |
T8310 |
T8311 |
amod |
neuronal,lines |
R4161 |
T8311 |
T8309 |
pobj |
lines,of |
R4162 |
T8312 |
T8310 |
cc |
and,neuronal |
R4163 |
T8313 |
T8310 |
conj |
glial,neuronal |
R4164 |
T8314 |
T8311 |
compound |
cell,lines |
R4165 |
T8315 |
T8297 |
punct |
.,confirmed |
R4166 |
T8508 |
T8507 |
cc |
and,Animals |
R4167 |
T8509 |
T8510 |
compound |
tissue,preparation |
R4168 |
T8510 |
T8507 |
conj |
preparation,Animals |
R4169 |
T8512 |
T8513 |
compound |
129SV,strain |
R4170 |
T8513 |
T8514 |
compound |
strain,mice |
R4171 |
T8514 |
T8515 |
nsubjpass |
mice,used |
R4172 |
T8516 |
T8515 |
auxpass |
were,used |
R4173 |
T8517 |
T8515 |
punct |
.,used |
R4174 |
T8519 |
T8520 |
det |
The,day |
R4175 |
T8520 |
T8521 |
nsubjpass |
day,defined |
R4176 |
T8522 |
T8523 |
prep |
on,confirmed |
R4177 |
T8523 |
T8520 |
relcl |
confirmed,day |
R4178 |
T8524 |
T8522 |
pobj |
which,on |
R4179 |
T8525 |
T8526 |
det |
the,plug |
R4180 |
T8526 |
T8523 |
nsubjpass |
plug,confirmed |
R4181 |
T8527 |
T8526 |
amod |
vaginal,plug |
R4182 |
T8528 |
T8523 |
auxpass |
was,confirmed |
R4183 |
T8529 |
T8521 |
auxpass |
was,defined |
R4184 |
T8530 |
T8521 |
prep |
as,defined |
R4185 |
T8531 |
T8530 |
pobj |
E0,as |
R4186 |
T8532 |
T8521 |
punct |
.,defined |
R4187 |
T8534 |
T8535 |
predet |
All,experiments |
R4188 |
T8535 |
T8538 |
nsubjpass |
experiments,performed |
R4189 |
T8536 |
T8535 |
det |
the,experiments |
R4190 |
T8537 |
T8535 |
compound |
animal,experiments |
R4191 |
T8539 |
T8538 |
auxpass |
were,performed |
R4192 |
T8540 |
T8538 |
prep |
in,performed |
R4193 |
T8541 |
T8540 |
pobj |
accordance,in |
R4194 |
T8542 |
T8541 |
prep |
with,accordance |
R4195 |
T8543 |
T8544 |
det |
the,Guide |
R4196 |
T8544 |
T8542 |
pobj |
Guide,with |
R4197 |
T8545 |
T8544 |
prep |
for,Guide |
R4198 |
T8546 |
T8547 |
det |
the,Care |
R4199 |
T8547 |
T8545 |
pobj |
Care,for |
R4200 |
T8548 |
T8547 |
cc |
and,Care |
R4201 |
T8549 |
T8547 |
conj |
Use,Care |
R4202 |
T8550 |
T8547 |
prep |
of,Care |
R4203 |
T8551 |
T8552 |
compound |
Laboratory,Animals |
R4204 |
T8552 |
T8550 |
pobj |
Animals,of |
R4205 |
T8553 |
T8554 |
punct |
(,NIH |
R4206 |
T8554 |
T8544 |
meta |
NIH,Guide |
R4207 |
T8555 |
T8554 |
nmod |
Publication,NIH |
R4208 |
T8556 |
T8554 |
nmod |
No.,NIH |
R4209 |
T8557 |
T8554 |
nummod |
85,NIH |
R4210 |
T8558 |
T8554 |
punct |
-,NIH |
R4211 |
T8559 |
T8554 |
nummod |
23,NIH |
R4212 |
T8560 |
T8554 |
punct |
),NIH |
R4213 |
T8561 |
T8538 |
punct |
.,performed |
R4214 |
T8563 |
T8564 |
amod |
Pregnant,females |
R4215 |
T8564 |
T8565 |
nsubjpass |
females,sacrificed |
R4216 |
T8566 |
T8565 |
auxpass |
were,sacrificed |
R4217 |
T8567 |
T8565 |
prep |
by,sacrificed |
R4218 |
T8568 |
T8569 |
amod |
cervical,dislocation |
R4219 |
T8569 |
T8567 |
pobj |
dislocation,by |
R4220 |
T8570 |
T8565 |
prep |
at,sacrificed |
R4221 |
T8571 |
T8572 |
amod |
various,intervals |
R4222 |
T8572 |
T8570 |
pobj |
intervals,at |
R4223 |
T8573 |
T8572 |
prep |
between,intervals |
R4224 |
T8574 |
T8573 |
pobj |
E5,between |
R4225 |
T8575 |
T8574 |
cc |
and,E5 |
R4226 |
T8576 |
T8574 |
conj |
E17,E5 |
R4227 |
T8577 |
T8565 |
punct |
.,sacrificed |
R4228 |
T8579 |
T8580 |
prep |
Before,embedded |
R4229 |
T8581 |
T8579 |
pobj |
E9,Before |
R4230 |
T8582 |
T8580 |
punct |
", ",embedded |
R4231 |
T8583 |
T8580 |
nsubjpass |
embryos,embedded |
R4232 |
T8584 |
T8580 |
auxpass |
were,embedded |
R4233 |
T8585 |
T8580 |
prep |
within,embedded |
R4234 |
T8586 |
T8587 |
det |
the,uterus |
R4235 |
T8587 |
T8585 |
pobj |
uterus,within |
R4236 |
T8588 |
T8587 |
amod |
maternal,uterus |
R4237 |
T8589 |
T8580 |
punct |
.,embedded |
R4238 |
T8591 |
T8592 |
prep |
After,removed |
R4239 |
T8593 |
T8591 |
pobj |
E9,After |
R4240 |
T8594 |
T8592 |
punct |
", ",removed |
R4241 |
T8595 |
T8592 |
nsubjpass |
embryos,removed |
R4242 |
T8596 |
T8592 |
auxpass |
were,removed |
R4243 |
T8597 |
T8592 |
prep |
from,removed |
R4244 |
T8598 |
T8599 |
poss |
their,membranes |
R4245 |
T8599 |
T8597 |
pobj |
membranes,from |
R4246 |
T8600 |
T8592 |
cc |
and,removed |
R4247 |
T8601 |
T8592 |
conj |
embedded,removed |
R4248 |
T8602 |
T8601 |
advmod |
separately,embedded |
R4249 |
T8603 |
T8592 |
punct |
.,removed |
R4250 |
T8605 |
T8606 |
nsubjpass |
Experiments,performed |
R4251 |
T8607 |
T8606 |
auxpass |
were,performed |
R4252 |
T8608 |
T8606 |
prep |
with,performed |
R4253 |
T8609 |
T8610 |
quantmod |
four,six |
R4254 |
T8610 |
T8612 |
nummod |
six,animals |
R4255 |
T8611 |
T8610 |
quantmod |
to,six |
R4256 |
T8612 |
T8608 |
pobj |
animals,with |
R4257 |
T8613 |
T8606 |
prep |
at,performed |
R4258 |
T8614 |
T8615 |
det |
each,stage |
R4259 |
T8615 |
T8613 |
pobj |
stage,at |
R4260 |
T8616 |
T8615 |
amod |
developmental,stage |
R4261 |
T8617 |
T8606 |
punct |
.,performed |
R4262 |
T8619 |
T8620 |
det |
The,brain |
R4263 |
T8620 |
T8622 |
nsubjpass |
brain,derived |
R4264 |
T8621 |
T8620 |
amod |
mature,brain |
R4265 |
T8623 |
T8620 |
acl |
used,brain |
R4266 |
T8624 |
T8622 |
auxpass |
was,derived |
R4267 |
T8625 |
T8622 |
prep |
from,derived |
R4268 |
T8626 |
T8625 |
pobj |
mice,from |
R4269 |
T8627 |
T8626 |
prep |
of,mice |
R4270 |
T8628 |
T8629 |
quantmod |
three,four |
R4271 |
T8629 |
T8631 |
nummod |
four,month |
R4272 |
T8630 |
T8629 |
quantmod |
to,four |
R4273 |
T8631 |
T8627 |
pobj |
month,of |
R4274 |
T8632 |
T8631 |
prep |
of,month |
R4275 |
T8633 |
T8632 |
pobj |
age,of |
R4276 |
T8634 |
T8622 |
punct |
.,derived |
R4277 |
T8636 |
T8637 |
det |
The,embryos |
R4278 |
T8637 |
T8638 |
nsubjpass |
embryos,fixed |
R4279 |
T8639 |
T8637 |
cc |
and,embryos |
R4280 |
T8640 |
T8637 |
conj |
tissues,embryos |
R4281 |
T8641 |
T8638 |
auxpass |
were,fixed |
R4282 |
T8642 |
T8638 |
prep |
in,fixed |
R4283 |
T8643 |
T8644 |
advmod |
freshly,prepared |
R4284 |
T8644 |
T8645 |
amod |
prepared,solution |
R4285 |
T8645 |
T8642 |
pobj |
solution,in |
R4286 |
T8646 |
T8647 |
nummod |
4,% |
R4287 |
T8647 |
T8645 |
compound |
%,solution |
R4288 |
T8648 |
T8645 |
compound |
paraformaldehyde,solution |
R4289 |
T8649 |
T8638 |
prep |
for,fixed |
R4290 |
T8650 |
T8651 |
quantmod |
2,12 |
R4291 |
T8651 |
T8653 |
nummod |
12,h |
R4292 |
T8652 |
T8651 |
punct |
–,12 |
R4293 |
T8653 |
T8649 |
pobj |
h,for |
R4294 |
T8654 |
T8638 |
prep |
depending,fixed |
R4295 |
T8655 |
T8654 |
prep |
on,depending |
R4296 |
T8656 |
T8657 |
det |
the,thickness |
R4297 |
T8657 |
T8655 |
pobj |
thickness,on |
R4298 |
T8658 |
T8657 |
compound |
tissue,thickness |
R4299 |
T8659 |
T8660 |
punct |
[,35 |
R4300 |
T8660 |
T8638 |
parataxis |
35,fixed |
R4301 |
T8661 |
T8660 |
punct |
],35 |
R4302 |
T8662 |
T8638 |
punct |
.,fixed |
R4303 |
T8664 |
T8665 |
nsubj |
They,dehydrated |
R4304 |
T8666 |
T8665 |
aux |
were,dehydrated |
R4305 |
T8667 |
T8665 |
prep |
with,dehydrated |
R4306 |
T8668 |
T8667 |
pobj |
ethanol,with |
R4307 |
T8669 |
T8665 |
cc |
and,dehydrated |
R4308 |
T8670 |
T8665 |
conj |
embedded,dehydrated |
R4309 |
T8671 |
T8670 |
prep |
in,embedded |
R4310 |
T8672 |
T8671 |
pobj |
paraffin,in |
R4311 |
T8673 |
T8665 |
prep |
according,dehydrated |
R4312 |
T8674 |
T8673 |
prep |
to,according |
R4313 |
T8675 |
T8676 |
amod |
standard,procedures |
R4314 |
T8676 |
T8674 |
pobj |
procedures,to |
R4315 |
T8677 |
T8665 |
punct |
.,dehydrated |
R4316 |
T8679 |
T8680 |
compound |
Microtome,sections |
R4317 |
T8680 |
T8681 |
nsubjpass |
sections,cut |
R4318 |
T8682 |
T8681 |
auxpass |
were,cut |
R4319 |
T8683 |
T8681 |
prep |
at,cut |
R4320 |
T8684 |
T8685 |
quantmod |
7,10 |
R4321 |
T8685 |
T8687 |
nummod |
10,μm |
R4322 |
T8686 |
T8685 |
punct |
–,10 |
R4323 |
T8687 |
T8683 |
pobj |
μm,at |
R4324 |
T8688 |
T8681 |
punct |
", ",cut |
R4325 |
T8689 |
T8681 |
conj |
placed,cut |
R4326 |
T8690 |
T8689 |
prep |
on,placed |
R4327 |
T8691 |
T8690 |
pobj |
slides,on |
R4328 |
T8692 |
T8689 |
cc |
and,placed |
R4329 |
T8693 |
T8689 |
conj |
stored,placed |
R4330 |
T8694 |
T8693 |
prep |
until,stored |
R4331 |
T8695 |
T8696 |
amod |
further,processing |
R4332 |
T8696 |
T8694 |
pobj |
processing,until |
R4333 |
T8697 |
T8681 |
punct |
.,cut |
R4334 |
T8723 |
T8724 |
amod |
Human,tissue |
R4335 |
T8725 |
T8724 |
compound |
brain,tissue |
R4336 |
T8727 |
T8728 |
nummod |
Five,brains |
R4337 |
T8728 |
T8731 |
nsubjpass |
brains,fixed |
R4338 |
T8729 |
T8728 |
amod |
human,brains |
R4339 |
T8730 |
T8728 |
compound |
autopsy,brains |
R4340 |
T8732 |
T8728 |
prep |
of,brains |
R4341 |
T8733 |
T8734 |
amod |
male,patients |
R4342 |
T8734 |
T8732 |
pobj |
patients,of |
R4343 |
T8735 |
T8733 |
cc |
and,male |
R4344 |
T8736 |
T8733 |
conj |
female,male |
R4345 |
T8737 |
T8734 |
prep |
without,patients |
R4346 |
T8738 |
T8739 |
det |
any,changes |
R4347 |
T8739 |
T8737 |
pobj |
changes,without |
R4348 |
T8740 |
T8739 |
amod |
neuropathological,changes |
R4349 |
T8741 |
T8742 |
punct |
(,h |
R4350 |
T8742 |
T8734 |
parataxis |
h,patients |
R4351 |
T8743 |
T8744 |
dep |
age,years |
R4352 |
T8744 |
T8742 |
dep |
years,h |
R4353 |
T8745 |
T8746 |
quantmod |
61,77 |
R4354 |
T8746 |
T8744 |
nummod |
77,years |
R4355 |
T8747 |
T8746 |
punct |
–,77 |
R4356 |
T8748 |
T8742 |
punct |
;,h |
R4357 |
T8749 |
T8750 |
advmod |
post,mortem |
R4358 |
T8750 |
T8751 |
advmod |
mortem,interval |
R4359 |
T8751 |
T8742 |
dep |
interval,h |
R4360 |
T8752 |
T8742 |
punct |
: ,h |
R4361 |
T8753 |
T8754 |
quantmod |
12,72 |
R4362 |
T8754 |
T8742 |
nummod |
72,h |
R4363 |
T8755 |
T8754 |
punct |
–,72 |
R4364 |
T8756 |
T8742 |
punct |
),h |
R4365 |
T8757 |
T8731 |
auxpass |
were,fixed |
R4366 |
T8758 |
T8731 |
prep |
in,fixed |
R4367 |
T8759 |
T8760 |
det |
an,solution |
R4368 |
T8760 |
T8758 |
pobj |
solution,in |
R4369 |
T8761 |
T8760 |
amod |
aqueous,solution |
R4370 |
T8762 |
T8760 |
prep |
of,solution |
R4371 |
T8763 |
T8762 |
pobj |
formaldehyde,of |
R4372 |
T8764 |
T8731 |
prep |
for,fixed |
R4373 |
T8765 |
T8766 |
nummod |
three,weeks |
R4374 |
T8766 |
T8764 |
pobj |
weeks,for |
R4375 |
T8767 |
T8731 |
punct |
.,fixed |
R4376 |
T8769 |
T8770 |
nsubjpass |
Blocks,embedded |
R4377 |
T8771 |
T8769 |
prep |
of,Blocks |
R4378 |
T8772 |
T8773 |
det |
the,neocortex |
R4379 |
T8773 |
T8771 |
pobj |
neocortex,of |
R4380 |
T8774 |
T8773 |
amod |
parietal,neocortex |
R4381 |
T8775 |
T8770 |
auxpass |
were,embedded |
R4382 |
T8776 |
T8770 |
prep |
in,embedded |
R4383 |
T8777 |
T8776 |
pobj |
paraffin,in |
R4384 |
T8778 |
T8770 |
cc |
and,embedded |
R4385 |
T8779 |
T8770 |
conj |
microtomed,embedded |
R4386 |
T8780 |
T8779 |
prep |
at,microtomed |
R4387 |
T8781 |
T8782 |
nummod |
12,μm |
R4388 |
T8782 |
T8783 |
compound |
μm,thickness |
R4389 |
T8783 |
T8780 |
pobj |
thickness,at |
R4390 |
T8784 |
T8770 |
punct |
.,embedded |
R4391 |
T8786 |
T8787 |
nsubjpass |
Sections,stained |
R4392 |
T8788 |
T8787 |
auxpass |
were,stained |
R4393 |
T8789 |
T8787 |
prep |
with,stained |
R4394 |
T8790 |
T8791 |
compound |
aldehydefuchsin,red |
R4395 |
T8791 |
T8789 |
pobj |
red,with |
R4396 |
T8792 |
T8791 |
compound |
Darrow,red |
R4397 |
T8793 |
T8791 |
punct |
-,red |
R4398 |
T8794 |
T8795 |
aux |
to,confirm |
R4399 |
T8795 |
T8787 |
advcl |
confirm,stained |
R4400 |
T8796 |
T8797 |
det |
the,absence |
R4401 |
T8797 |
T8795 |
dobj |
absence,confirm |
R4402 |
T8798 |
T8797 |
prep |
of,absence |
R4403 |
T8799 |
T8800 |
amod |
neuropathological,alterations |
R4404 |
T8800 |
T8798 |
pobj |
alterations,of |
R4405 |
T8801 |
T8787 |
punct |
.,stained |
R4406 |
T8803 |
T8804 |
det |
All,procedures |
R4407 |
T8804 |
T8805 |
nsubjpass |
procedures,conducted |
R4408 |
T8806 |
T8805 |
auxpass |
were,conducted |
R4409 |
T8807 |
T8805 |
prep |
in,conducted |
R4410 |
T8808 |
T8807 |
pobj |
accordance,in |
R4411 |
T8809 |
T8808 |
prep |
with,accordance |
R4412 |
T8810 |
T8811 |
det |
the,Declaration |
R4413 |
T8811 |
T8809 |
pobj |
Declaration,with |
R4414 |
T8812 |
T8811 |
prep |
of,Declaration |
R4415 |
T8813 |
T8812 |
pobj |
Helsinki,of |
R4416 |
T8814 |
T8805 |
cc |
and,conducted |
R4417 |
T8815 |
T8805 |
conj |
approved,conducted |
R4418 |
T8816 |
T8815 |
agent |
by,approved |
R4419 |
T8817 |
T8818 |
det |
the,committee |
R4420 |
T8818 |
T8816 |
pobj |
committee,by |
R4421 |
T8819 |
T8818 |
compound |
ethics,committee |
R4422 |
T8820 |
T8818 |
prep |
of,committee |
R4423 |
T8821 |
T8822 |
det |
the,University |
R4424 |
T8822 |
T8823 |
nmod |
University,Center |
R4425 |
T8823 |
T8820 |
pobj |
Center,of |
R4426 |
T8824 |
T8822 |
prep |
of,University |
R4427 |
T8825 |
T8824 |
pobj |
Bonn,of |
R4428 |
T8826 |
T8823 |
amod |
Medical,Center |
R4429 |
T8827 |
T8805 |
punct |
.,conducted |
R4430 |
T8972 |
T8973 |
nsubj |
Antibodies,were |
R4431 |
T8974 |
T8972 |
acl |
used,Antibodies |
R4432 |
T8975 |
T8974 |
prep |
in,used |
R4433 |
T8976 |
T8977 |
det |
this,study |
R4434 |
T8977 |
T8975 |
pobj |
study,in |
R4435 |
T8978 |
T8979 |
nmod |
mAb,203 |
R4436 |
T8979 |
T8973 |
attr |
203,were |
R4437 |
T8980 |
T8981 |
punct |
–,217 |
R4438 |
T8981 |
T8979 |
prep |
217,203 |
R4439 |
T8982 |
T8979 |
acl |
directed,203 |
R4440 |
T8983 |
T8982 |
prep |
against,directed |
R4441 |
T8984 |
T8985 |
det |
the,domain |
R4442 |
T8985 |
T8983 |
pobj |
domain,against |
R4443 |
T8986 |
T8985 |
compound |
core,domain |
R4444 |
T8987 |
T8985 |
prep |
of,domain |
R4445 |
T8988 |
T8989 |
compound |
mouse,A7 |
R4446 |
T8989 |
T8987 |
pobj |
A7,of |
R4447 |
T8990 |
T8989 |
compound |
Annexin,A7 |
R4448 |
T8991 |
T8992 |
punct |
[,6 |
R4449 |
T8992 |
T8989 |
parataxis |
6,A7 |
R4450 |
T8993 |
T8992 |
punct |
],6 |
R4451 |
T8994 |
T8995 |
punct |
(,1 |
R4452 |
T8995 |
T8989 |
parataxis |
1,A7 |
R4453 |
T8996 |
T8997 |
amod |
purified,IgG |
R4454 |
T8997 |
T8995 |
dep |
IgG,1 |
R4455 |
T8998 |
T8995 |
punct |
", ",1 |
R4456 |
T8999 |
T9000 |
punct |
:,50 |
R4457 |
T9000 |
T8995 |
prep |
50,1 |
R4458 |
T9001 |
T8995 |
punct |
),1 |
R4459 |
T9002 |
T9003 |
advmod |
specifically,recognizing |
R4460 |
T9003 |
T8973 |
advcl |
recognizing,were |
R4461 |
T9004 |
T9005 |
det |
both,isoforms |
R4462 |
T9005 |
T9003 |
dobj |
isoforms,recognizing |
R4463 |
T9006 |
T9007 |
compound |
Annexin,A7 |
R4464 |
T9007 |
T9005 |
compound |
A7,isoforms |
R4465 |
T9008 |
T9009 |
punct |
[,10 |
R4466 |
T9009 |
T9005 |
parataxis |
10,isoforms |
R4467 |
T9010 |
T9009 |
punct |
[,10 |
R4468 |
T9011 |
T9009 |
nummod |
6,10 |
R4469 |
T9012 |
T9009 |
punct |
",",10 |
R4470 |
T9013 |
T9009 |
punct |
],10 |
R4471 |
T9014 |
T9009 |
punct |
", ",10 |
R4472 |
T9015 |
T9009 |
cc |
and,10 |
R4473 |
T9016 |
T9017 |
nsubj |
data,shown |
R4474 |
T9017 |
T9009 |
conj |
shown,10 |
R4475 |
T9018 |
T9017 |
neg |
not,shown |
R4476 |
T9019 |
T9017 |
punct |
],shown |
R4477 |
T9020 |
T9005 |
punct |
", ",isoforms |
R4478 |
T9021 |
T9022 |
det |
a,antibody |
R4479 |
T9022 |
T9005 |
conj |
antibody,isoforms |
R4480 |
T9023 |
T9022 |
amod |
polyclonal,antibody |
R4481 |
T9024 |
T9022 |
prep |
against,antibody |
R4482 |
T9025 |
T9026 |
compound |
mouse,A7 |
R4483 |
T9026 |
T9024 |
pobj |
A7,against |
R4484 |
T9027 |
T9026 |
compound |
Annexin,A7 |
R4485 |
T9028 |
T9029 |
punct |
[,10 |
R4486 |
T9029 |
T9022 |
parataxis |
10,antibody |
R4487 |
T9030 |
T9029 |
punct |
],10 |
R4488 |
T9031 |
T9032 |
punct |
(,1 |
R4489 |
T9032 |
T9022 |
parataxis |
1,antibody |
R4490 |
T9033 |
T9034 |
punct |
:,200 |
R4491 |
T9034 |
T9032 |
prep |
200,1 |
R4492 |
T9035 |
T9032 |
punct |
),1 |
R4493 |
T9036 |
T9022 |
punct |
", ",antibody |
R4494 |
T9037 |
T9038 |
det |
a,antiserum |
R4495 |
T9038 |
T9022 |
conj |
antiserum,antibody |
R4496 |
T9039 |
T9038 |
amod |
polyclonal,antiserum |
R4497 |
T9040 |
T9038 |
acl |
raised,antiserum |
R4498 |
T9041 |
T9040 |
prep |
against,raised |
R4499 |
T9042 |
T9043 |
det |
the,protein |
R4500 |
T9043 |
T9041 |
pobj |
protein,against |
R4501 |
T9044 |
T9045 |
npadvmod |
astrocyte,specific |
R4502 |
T9045 |
T9043 |
amod |
specific,protein |
R4503 |
T9046 |
T9045 |
punct |
-,specific |
R4504 |
T9047 |
T9048 |
amod |
intermediate,filament |
R4505 |
T9048 |
T9043 |
nmod |
filament,protein |
R4506 |
T9049 |
T9043 |
amod |
glial,protein |
R4507 |
T9050 |
T9043 |
amod |
fibrillary,protein |
R4508 |
T9051 |
T9043 |
amod |
acidic,protein |
R4509 |
T9052 |
T9053 |
punct |
(,DAKO |
R4510 |
T9053 |
T9038 |
parataxis |
DAKO,antiserum |
R4511 |
T9054 |
T9053 |
dep |
GFAP,DAKO |
R4512 |
T9055 |
T9053 |
punct |
;,DAKO |
R4513 |
T9056 |
T9053 |
punct |
", ",DAKO |
R4514 |
T9057 |
T9053 |
appos |
1,DAKO |
R4515 |
T9058 |
T9059 |
punct |
:,400 |
R4516 |
T9059 |
T9057 |
prep |
400,1 |
R4517 |
T9060 |
T9053 |
punct |
),DAKO |
R4518 |
T9061 |
T9038 |
punct |
", ",antiserum |
R4519 |
T9062 |
T9063 |
amod |
anti-β,tubulin |
R4520 |
T9063 |
T9038 |
conj |
tubulin,antiserum |
R4521 |
T9064 |
T9063 |
punct |
-,tubulin |
R4522 |
T9065 |
T9066 |
punct |
(,1 |
R4523 |
T9066 |
T9063 |
parataxis |
1,tubulin |
R4524 |
T9067 |
T9066 |
dep |
Sigma,1 |
R4525 |
T9068 |
T9066 |
punct |
", ",1 |
R4526 |
T9069 |
T9070 |
punct |
:,1000 |
R4527 |
T9070 |
T9066 |
prep |
1000,1 |
R4528 |
T9071 |
T9066 |
punct |
),1 |
R4529 |
T9072 |
T9063 |
punct |
", ",tubulin |
R4530 |
T9073 |
T9063 |
conj |
anti-Emerin,tubulin |
R4531 |
T9074 |
T9075 |
punct |
(,1 |
R4532 |
T9075 |
T9073 |
parataxis |
1,anti-Emerin |
R4533 |
T9076 |
T9075 |
dep |
novocastra,1 |
R4534 |
T9077 |
T9075 |
punct |
", ",1 |
R4535 |
T9078 |
T9079 |
punct |
:,2000 |
R4536 |
T9079 |
T9075 |
prep |
2000,1 |
R4537 |
T9080 |
T9075 |
punct |
),1 |
R4538 |
T9081 |
T9073 |
punct |
", ",anti-Emerin |
R4539 |
T9082 |
T9073 |
cc |
and,anti-Emerin |
R4540 |
T9083 |
T9084 |
amod |
polyclonal,anti-LAP2α |
R4541 |
T9084 |
T9073 |
conj |
anti-LAP2α,anti-Emerin |
R4542 |
T9085 |
T9084 |
punct |
(,anti-LAP2α |
R4543 |
T9086 |
T9087 |
advmod |
kindly,provided |
R4544 |
T9087 |
T9084 |
acl |
provided,anti-LAP2α |
R4545 |
T9088 |
T9087 |
agent |
by,provided |
R4546 |
T9089 |
T9090 |
compound |
Ronald,Foisner |
R4547 |
T9090 |
T9088 |
pobj |
Foisner,by |
R4548 |
T9091 |
T9092 |
punct |
", ",1 |
R4549 |
T9092 |
T9084 |
parataxis |
1,anti-LAP2α |
R4550 |
T9093 |
T9094 |
punct |
:,5000 |
R4551 |
T9094 |
T9092 |
prep |
5000,1 |
R4552 |
T9095 |
T9092 |
punct |
),1 |
R4553 |
T9096 |
T8973 |
punct |
.,were |
R4554 |
T9606 |
T9607 |
compound |
Immunofluorescence,microscopy |
R4555 |
T9609 |
T9610 |
amod |
Immunohistochemical,staining |
R4556 |
T9610 |
T9611 |
nsubj |
staining,was |
R4557 |
T9612 |
T9610 |
acl |
using,staining |
R4558 |
T9613 |
T9614 |
nmod |
mAb,203 |
R4559 |
T9614 |
T9612 |
dobj |
203,using |
R4560 |
T9615 |
T9616 |
punct |
–,217 |
R4561 |
T9616 |
T9614 |
prep |
217,203 |
R4562 |
T9617 |
T9612 |
prep |
as,using |
R4563 |
T9618 |
T9619 |
det |
the,antibody |
R4564 |
T9619 |
T9617 |
pobj |
antibody,as |
R4565 |
T9620 |
T9619 |
amod |
primary,antibody |
R4566 |
T9621 |
T9611 |
prep |
with,was |
R4567 |
T9622 |
T9623 |
npadvmod |
AlexaFluor488,conjugated |
R4568 |
T9623 |
T9625 |
amod |
conjugated,IgG |
R4569 |
T9624 |
T9623 |
punct |
-,conjugated |
R4570 |
T9625 |
T9621 |
pobj |
IgG,with |
R4571 |
T9626 |
T9625 |
nmod |
goat,IgG |
R4572 |
T9627 |
T9625 |
amod |
anti-mouse,IgG |
R4573 |
T9628 |
T9629 |
punct |
(,Probes |
R4574 |
T9629 |
T9625 |
parataxis |
Probes,IgG |
R4575 |
T9630 |
T9629 |
compound |
Molecular,Probes |
R4576 |
T9631 |
T9629 |
punct |
", ",Probes |
R4577 |
T9632 |
T9629 |
npadvmod |
Leiden,Probes |
R4578 |
T9633 |
T9629 |
punct |
", ",Probes |
R4579 |
T9634 |
T9635 |
det |
The,lands |
R4580 |
T9635 |
T9629 |
npadvmod |
lands,Probes |
R4581 |
T9636 |
T9635 |
compound |
Nether,lands |
R4582 |
T9637 |
T9635 |
punct |
-,lands |
R4583 |
T9638 |
T9629 |
punct |
),Probes |
R4584 |
T9639 |
T9625 |
cc |
or,IgG |
R4585 |
T9640 |
T9641 |
npadvmod |
Cy3,labeled |
R4586 |
T9641 |
T9643 |
amod |
labeled,IgG |
R4587 |
T9642 |
T9641 |
punct |
-,labeled |
R4588 |
T9643 |
T9625 |
conj |
IgG,IgG |
R4589 |
T9644 |
T9643 |
nmod |
goat,IgG |
R4590 |
T9645 |
T9643 |
amod |
anti-mouse,IgG |
R4591 |
T9646 |
T9625 |
prep |
as,IgG |
R4592 |
T9647 |
T9648 |
det |
the,antibodies |
R4593 |
T9648 |
T9646 |
pobj |
antibodies,as |
R4594 |
T9649 |
T9648 |
amod |
secondary,antibodies |
R4595 |
T9650 |
T9611 |
punct |
.,was |
R4596 |
T9652 |
T9653 |
amod |
Polyclonal,antibody |
R4597 |
T9653 |
T9656 |
nsubjpass |
antibody,combined |
R4598 |
T9654 |
T9655 |
amod |
anti-Annexin,A7 |
R4599 |
T9655 |
T9653 |
compound |
A7,antibody |
R4600 |
T9657 |
T9653 |
cc |
and,antibody |
R4601 |
T9658 |
T9659 |
nmod |
anti-GFAP,antibody |
R4602 |
T9659 |
T9653 |
conj |
antibody,antibody |
R4603 |
T9660 |
T9659 |
punct |
-,antibody |
R4604 |
T9661 |
T9656 |
auxpass |
were,combined |
R4605 |
T9662 |
T9656 |
prep |
with,combined |
R4606 |
T9663 |
T9664 |
compound |
Alexa,Fluor |
R4607 |
T9664 |
T9665 |
npadvmod |
Fluor,conjugated |
R4608 |
T9665 |
T9668 |
amod |
conjugated,IgG |
R4609 |
T9666 |
T9664 |
nummod |
568,Fluor |
R4610 |
T9667 |
T9665 |
punct |
-,conjugated |
R4611 |
T9668 |
T9662 |
pobj |
IgG,with |
R4612 |
T9669 |
T9668 |
nmod |
goat,IgG |
R4613 |
T9670 |
T9668 |
amod |
anti-rabbit,IgG |
R4614 |
T9671 |
T9672 |
punct |
(,Probes |
R4615 |
T9672 |
T9668 |
parataxis |
Probes,IgG |
R4616 |
T9673 |
T9672 |
compound |
Molecular,Probes |
R4617 |
T9674 |
T9672 |
punct |
),Probes |
R4618 |
T9675 |
T9656 |
punct |
.,combined |
R4619 |
T9677 |
T9678 |
det |
The,labelling |
R4620 |
T9678 |
T9680 |
nsubjpass |
labelling,confirmed |
R4621 |
T9679 |
T9678 |
amod |
specific,labelling |
R4622 |
T9681 |
T9678 |
prep |
of,labelling |
R4623 |
T9682 |
T9683 |
compound |
Annexin,A7 |
R4624 |
T9683 |
T9681 |
pobj |
A7,of |
R4625 |
T9684 |
T9678 |
acl |
detected,labelling |
R4626 |
T9685 |
T9684 |
prep |
with,detected |
R4627 |
T9686 |
T9687 |
det |
the,antibodies |
R4628 |
T9687 |
T9685 |
pobj |
antibodies,with |
R4629 |
T9688 |
T9689 |
amod |
anti-mouse,IgG |
R4630 |
T9689 |
T9687 |
nmod |
IgG,antibodies |
R4631 |
T9690 |
T9689 |
punct |
-,IgG |
R4632 |
T9691 |
T9687 |
amod |
secondary,antibodies |
R4633 |
T9692 |
T9684 |
prep |
in,detected |
R4634 |
T9693 |
T9694 |
compound |
mouse,sections |
R4635 |
T9694 |
T9692 |
pobj |
sections,in |
R4636 |
T9695 |
T9694 |
compound |
tissue,sections |
R4637 |
T9696 |
T9680 |
auxpass |
was,confirmed |
R4638 |
T9697 |
T9680 |
advcl |
using,confirmed |
R4639 |
T9698 |
T9699 |
amod |
primary,antibodies |
R4640 |
T9699 |
T9697 |
dobj |
antibodies,using |
R4641 |
T9700 |
T9699 |
punct |
", ",antibodies |
R4642 |
T9701 |
T9702 |
dep |
which,coupled |
R4643 |
T9702 |
T9699 |
relcl |
coupled,antibodies |
R4644 |
T9703 |
T9702 |
auxpass |
were,coupled |
R4645 |
T9704 |
T9702 |
advmod |
directly,coupled |
R4646 |
T9705 |
T9702 |
prep |
to,coupled |
R4647 |
T9706 |
T9707 |
compound |
Alexa,Fluor488 |
R4648 |
T9707 |
T9705 |
pobj |
Fluor488,to |
R4649 |
T9708 |
T9680 |
punct |
.,confirmed |
R4650 |
T9710 |
T9711 |
det |
The,sections |
R4651 |
T9711 |
T9715 |
nsubjpass |
sections,treated |
R4652 |
T9712 |
T9713 |
npadvmod |
paraffin,embedded |
R4653 |
T9713 |
T9711 |
amod |
embedded,sections |
R4654 |
T9714 |
T9713 |
punct |
-,embedded |
R4655 |
T9716 |
T9715 |
auxpass |
were,treated |
R4656 |
T9717 |
T9715 |
advmod |
first,treated |
R4657 |
T9718 |
T9719 |
nummod |
three,times |
R4658 |
T9719 |
T9715 |
npadvmod |
times,treated |
R4659 |
T9720 |
T9715 |
prep |
for,treated |
R4660 |
T9721 |
T9722 |
nummod |
2,min |
R4661 |
T9722 |
T9720 |
pobj |
min,for |
R4662 |
T9723 |
T9715 |
prep |
in,treated |
R4663 |
T9724 |
T9723 |
pobj |
xylene,in |
R4664 |
T9725 |
T9726 |
aux |
to,remove |
R4665 |
T9726 |
T9715 |
advcl |
remove,treated |
R4666 |
T9727 |
T9726 |
dobj |
paraffin,remove |
R4667 |
T9728 |
T9715 |
cc |
and,treated |
R4668 |
T9729 |
T9730 |
advmod |
then,rehydrated |
R4669 |
T9730 |
T9715 |
conj |
rehydrated,treated |
R4670 |
T9731 |
T9730 |
npadvmod |
step,rehydrated |
R4671 |
T9732 |
T9731 |
prep |
by,step |
R4672 |
T9733 |
T9732 |
pobj |
step,by |
R4673 |
T9734 |
T9730 |
prep |
with,rehydrated |
R4674 |
T9735 |
T9736 |
amod |
descending,concentrations |
R4675 |
T9736 |
T9734 |
pobj |
concentrations,with |
R4676 |
T9737 |
T9736 |
prep |
of,concentrations |
R4677 |
T9738 |
T9737 |
pobj |
ethanol,of |
R4678 |
T9739 |
T9730 |
prep |
for,rehydrated |
R4679 |
T9740 |
T9741 |
compound |
antibody,staining |
R4680 |
T9741 |
T9739 |
pobj |
staining,for |
R4681 |
T9742 |
T9743 |
punct |
[,35 |
R4682 |
T9743 |
T9730 |
parataxis |
35,rehydrated |
R4683 |
T9744 |
T9743 |
punct |
],35 |
R4684 |
T9745 |
T9715 |
punct |
.,treated |
R4685 |
T9747 |
T9748 |
prep |
After,incubated |
R4686 |
T9749 |
T9750 |
nummod |
three,washings |
R4687 |
T9750 |
T9747 |
pobj |
washings,After |
R4688 |
T9751 |
T9750 |
prep |
in,washings |
R4689 |
T9752 |
T9751 |
pobj |
PBS,in |
R4690 |
T9753 |
T9754 |
punct |
(,NaCl |
R4691 |
T9754 |
T9750 |
parataxis |
NaCl,washings |
R4692 |
T9755 |
T9756 |
nummod |
137,mM |
R4693 |
T9756 |
T9754 |
compound |
mM,NaCl |
R4694 |
T9757 |
T9754 |
punct |
", ",NaCl |
R4695 |
T9758 |
T9759 |
nummod |
2.7,mM |
R4696 |
T9759 |
T9760 |
compound |
mM,KCl |
R4697 |
T9760 |
T9754 |
appos |
KCl,NaCl |
R4698 |
T9761 |
T9754 |
punct |
", ",NaCl |
R4699 |
T9762 |
T9763 |
nummod |
8.1,mM |
R4700 |
T9763 |
T9764 |
compound |
mM,Na2HPO4 |
R4701 |
T9764 |
T9754 |
appos |
Na2HPO4,NaCl |
R4702 |
T9765 |
T9754 |
punct |
", ",NaCl |
R4703 |
T9766 |
T9767 |
nummod |
1.5,mM |
R4704 |
T9767 |
T9768 |
compound |
mM,KH2PO4 |
R4705 |
T9768 |
T9754 |
appos |
KH2PO4,NaCl |
R4706 |
T9769 |
T9754 |
punct |
", ",NaCl |
R4707 |
T9770 |
T9754 |
npadvmod |
pH,NaCl |
R4708 |
T9771 |
T9770 |
nummod |
7.4,pH |
R4709 |
T9772 |
T9754 |
punct |
),NaCl |
R4710 |
T9773 |
T9748 |
punct |
", ",incubated |
R4711 |
T9774 |
T9775 |
det |
the,sections |
R4712 |
T9775 |
T9748 |
nsubjpass |
sections,incubated |
R4713 |
T9776 |
T9775 |
compound |
tissue,sections |
R4714 |
T9777 |
T9748 |
auxpass |
were,incubated |
R4715 |
T9778 |
T9748 |
prep |
in,incubated |
R4716 |
T9779 |
T9778 |
pobj |
PBS,in |
R4717 |
T9780 |
T9779 |
acl |
containing,PBS |
R4718 |
T9781 |
T9782 |
nummod |
0.1,% |
R4719 |
T9782 |
T9783 |
compound |
%,trypsin |
R4720 |
T9783 |
T9780 |
dobj |
trypsin,containing |
R4721 |
T9784 |
T9748 |
prep |
for,incubated |
R4722 |
T9785 |
T9786 |
nummod |
10,min |
R4723 |
T9786 |
T9784 |
pobj |
min,for |
R4724 |
T9787 |
T9748 |
prep |
at,incubated |
R4725 |
T9788 |
T9789 |
compound |
room,temperature |
R4726 |
T9789 |
T9787 |
pobj |
temperature,at |
R4727 |
T9790 |
T9791 |
aux |
to,free |
R4728 |
T9791 |
T9748 |
advcl |
free,incubated |
R4729 |
T9792 |
T9793 |
npadvmod |
formaldehyde,crosslinked |
R4730 |
T9793 |
T9795 |
amod |
crosslinked,epitopes |
R4731 |
T9794 |
T9793 |
punct |
-,crosslinked |
R4732 |
T9795 |
T9791 |
dobj |
epitopes,free |
R4733 |
T9796 |
T9797 |
punct |
[,15 |
R4734 |
T9797 |
T9748 |
parataxis |
15,incubated |
R4735 |
T9798 |
T9799 |
punct |
-,17 |
R4736 |
T9799 |
T9797 |
prep |
17,15 |
R4737 |
T9800 |
T9797 |
punct |
],15 |
R4738 |
T9801 |
T9748 |
punct |
.,incubated |
R4739 |
T9803 |
T9804 |
prep |
After,blocked |
R4740 |
T9805 |
T9806 |
amod |
additional,washes |
R4741 |
T9806 |
T9803 |
pobj |
washes,After |
R4742 |
T9807 |
T9806 |
nummod |
three,washes |
R4743 |
T9808 |
T9806 |
prep |
with,washes |
R4744 |
T9809 |
T9808 |
pobj |
PBS,with |
R4745 |
T9810 |
T9804 |
punct |
", ",blocked |
R4746 |
T9811 |
T9812 |
amod |
non-specific,sites |
R4747 |
T9812 |
T9804 |
nsubjpass |
sites,blocked |
R4748 |
T9813 |
T9812 |
compound |
binding,sites |
R4749 |
T9814 |
T9804 |
auxpass |
were,blocked |
R4750 |
T9815 |
T9804 |
prep |
by,blocked |
R4751 |
T9816 |
T9815 |
pcomp |
incubating,by |
R4752 |
T9817 |
T9818 |
det |
the,slides |
R4753 |
T9818 |
T9816 |
dobj |
slides,incubating |
R4754 |
T9819 |
T9816 |
prep |
with,incubating |
R4755 |
T9820 |
T9819 |
pobj |
PBG,with |
R4756 |
T9821 |
T9822 |
punct |
(,BSA |
R4757 |
T9822 |
T9820 |
parataxis |
BSA,PBG |
R4758 |
T9823 |
T9824 |
nummod |
0.5,% |
R4759 |
T9824 |
T9822 |
compound |
%,BSA |
R4760 |
T9825 |
T9822 |
punct |
", ",BSA |
R4761 |
T9826 |
T9827 |
nummod |
0.045,% |
R4762 |
T9827 |
T9828 |
compound |
%,gelatine |
R4763 |
T9828 |
T9822 |
appos |
gelatine,BSA |
R4764 |
T9829 |
T9828 |
compound |
fish,gelatine |
R4765 |
T9830 |
T9822 |
prep |
in,BSA |
R4766 |
T9831 |
T9830 |
pobj |
PBS,in |
R4767 |
T9832 |
T9822 |
punct |
),BSA |
R4768 |
T9833 |
T9816 |
prep |
in,incubating |
R4769 |
T9834 |
T9835 |
nummod |
10,% |
R4770 |
T9835 |
T9833 |
pobj |
%,in |
R4771 |
T9836 |
T9837 |
amod |
normal,serum |
R4772 |
T9837 |
T9835 |
appos |
serum,% |
R4773 |
T9838 |
T9837 |
compound |
goat,serum |
R4774 |
T9839 |
T9837 |
punct |
(,serum |
R4775 |
T9840 |
T9837 |
appos |
NGS,serum |
R4776 |
T9841 |
T9835 |
punct |
),% |
R4777 |
T9842 |
T9816 |
prep |
for,incubating |
R4778 |
T9843 |
T9844 |
nummod |
2,h |
R4779 |
T9844 |
T9842 |
pobj |
h,for |
R4780 |
T9845 |
T9804 |
punct |
.,blocked |
R4781 |
T9847 |
T9848 |
nsubjpass |
Incubation,diluted |
R4782 |
T9849 |
T9847 |
prep |
with,Incubation |
R4783 |
T9850 |
T9851 |
det |
the,antibodies |
R4784 |
T9851 |
T9849 |
pobj |
antibodies,with |
R4785 |
T9852 |
T9851 |
amod |
primary,antibodies |
R4786 |
T9853 |
T9848 |
auxpass |
was,diluted |
R4787 |
T9854 |
T9848 |
aux |
performed,diluted |
R4788 |
T9855 |
T9848 |
prep |
at,diluted |
R4789 |
T9856 |
T9857 |
nummod |
4,°C |
R4790 |
T9857 |
T9855 |
pobj |
°C,at |
R4791 |
T9858 |
T9848 |
advmod |
overnight,diluted |
R4792 |
T9859 |
T9848 |
prep |
in,diluted |
R4793 |
T9860 |
T9859 |
pobj |
PBG,in |
R4794 |
T9861 |
T9860 |
acl |
supplemented,PBG |
R4795 |
T9862 |
T9861 |
prep |
with,supplemented |
R4796 |
T9863 |
T9864 |
nummod |
1,% |
R4797 |
T9864 |
T9865 |
compound |
%,NGS |
R4798 |
T9865 |
T9862 |
pobj |
NGS,with |
R4799 |
T9866 |
T9848 |
punct |
.,diluted |
R4800 |
T9868 |
T9869 |
prep |
After,incubated |
R4801 |
T9870 |
T9871 |
nummod |
five,washes |
R4802 |
T9871 |
T9868 |
pobj |
washes,After |
R4803 |
T9872 |
T9871 |
prep |
in,washes |
R4804 |
T9873 |
T9872 |
pobj |
PBG,in |
R4805 |
T9874 |
T9871 |
prep |
for,washes |
R4806 |
T9875 |
T9876 |
nummod |
5,min |
R4807 |
T9876 |
T9874 |
pobj |
min,for |
R4808 |
T9877 |
T9876 |
appos |
each,min |
R4809 |
T9878 |
T9869 |
punct |
", ",incubated |
R4810 |
T9879 |
T9880 |
det |
the,slides |
R4811 |
T9880 |
T9869 |
nsubjpass |
slides,incubated |
R4812 |
T9881 |
T9869 |
auxpass |
were,incubated |
R4813 |
T9882 |
T9869 |
prep |
for,incubated |
R4814 |
T9883 |
T9884 |
nummod |
1,h |
R4815 |
T9884 |
T9882 |
pobj |
h,for |
R4816 |
T9885 |
T9869 |
prep |
with,incubated |
R4817 |
T9886 |
T9887 |
det |
the,antibody |
R4818 |
T9887 |
T9885 |
pobj |
antibody,with |
R4819 |
T9888 |
T9887 |
amod |
secondary,antibody |
R4820 |
T9889 |
T9869 |
prep |
at,incubated |
R4821 |
T9890 |
T9891 |
compound |
room,temperature |
R4822 |
T9891 |
T9889 |
pobj |
temperature,at |
R4823 |
T9892 |
T9869 |
punct |
", ",incubated |
R4824 |
T9893 |
T9869 |
conj |
washed,incubated |
R4825 |
T9894 |
T9895 |
nummod |
three,times |
R4826 |
T9895 |
T9893 |
npadvmod |
times,washed |
R4827 |
T9896 |
T9893 |
prep |
in,washed |
R4828 |
T9897 |
T9896 |
pobj |
PBG,in |
R4829 |
T9898 |
T9893 |
cc |
and,washed |
R4830 |
T9899 |
T9900 |
nummod |
three,times |
R4831 |
T9900 |
T9893 |
conj |
times,washed |
R4832 |
T9901 |
T9900 |
prep |
in,times |
R4833 |
T9902 |
T9901 |
pobj |
PBS,in |
R4834 |
T9903 |
T9893 |
punct |
", ",washed |
R4835 |
T9904 |
T9905 |
nummod |
5,min |
R4836 |
T9905 |
T9893 |
npadvmod |
min,washed |
R4837 |
T9906 |
T9905 |
appos |
each,min |
R4838 |
T9907 |
T9893 |
punct |
", ",washed |
R4839 |
T9908 |
T9893 |
conj |
rinsed,washed |
R4840 |
T9909 |
T9908 |
prep |
in,rinsed |
R4841 |
T9910 |
T9909 |
pobj |
water,in |
R4842 |
T9911 |
T9908 |
cc |
and,rinsed |
R4843 |
T9912 |
T9908 |
conj |
embedded,rinsed |
R4844 |
T9913 |
T9912 |
prep |
in,embedded |
R4845 |
T9914 |
T9913 |
pobj |
gelvatol,in |
R4846 |
T9915 |
T9916 |
punct |
[,36 |
R4847 |
T9916 |
T9912 |
parataxis |
36,embedded |
R4848 |
T9917 |
T9916 |
punct |
],36 |
R4849 |
T9918 |
T9869 |
punct |
.,incubated |
R4850 |
T9920 |
T9921 |
prep |
For,incubated |
R4851 |
T9922 |
T9920 |
pobj |
control,For |
R4852 |
T9923 |
T9921 |
punct |
", ",incubated |
R4853 |
T9924 |
T9921 |
nsubjpass |
sections,incubated |
R4854 |
T9925 |
T9921 |
auxpass |
were,incubated |
R4855 |
T9926 |
T9927 |
advmod |
only,with |
R4856 |
T9927 |
T9921 |
prep |
with,incubated |
R4857 |
T9928 |
T9929 |
det |
the,antibody |
R4858 |
T9929 |
T9927 |
pobj |
antibody,with |
R4859 |
T9930 |
T9929 |
amod |
secondary,antibody |
R4860 |
T9931 |
T9921 |
cc |
or,incubated |
R4861 |
T9932 |
T9933 |
nsubjpass |
sections,used |
R4862 |
T9933 |
T9921 |
conj |
used,incubated |
R4863 |
T9934 |
T9932 |
acl |
obtained,sections |
R4864 |
T9935 |
T9934 |
prep |
from,obtained |
R4865 |
T9936 |
T9937 |
det |
an,mouse |
R4866 |
T9937 |
T9935 |
pobj |
mouse,from |
R4867 |
T9938 |
T9939 |
compound |
annexin,A7 |
R4868 |
T9939 |
T9937 |
compound |
A7,mouse |
R4869 |
T9940 |
T9937 |
compound |
knockout,mouse |
R4870 |
T9941 |
T9933 |
auxpass |
were,used |
R4871 |
T9942 |
T9943 |
punct |
[,10 |
R4872 |
T9943 |
T9933 |
parataxis |
10,used |
R4873 |
T9944 |
T9943 |
punct |
],10 |
R4874 |
T9945 |
T9921 |
punct |
.,incubated |
R4875 |
T9947 |
T9948 |
nsubjpass |
Samples,examined |
R4876 |
T9949 |
T9948 |
auxpass |
were,examined |
R4877 |
T9950 |
T9948 |
prep |
with,examined |
R4878 |
T9951 |
T9952 |
det |
a,microscope |
R4879 |
T9952 |
T9950 |
pobj |
microscope,with |
R4880 |
T9953 |
T9952 |
amod |
confocal,microscope |
R4881 |
T9954 |
T9952 |
compound |
laser,microscope |
R4882 |
T9955 |
T9952 |
compound |
scan,microscope |
R4883 |
T9956 |
T9957 |
punct |
(,SP |
R4884 |
T9957 |
T9952 |
parataxis |
SP,microscope |
R4885 |
T9958 |
T9957 |
compound |
Leica,SP |
R4886 |
T9959 |
T9957 |
compound |
TCS,SP |
R4887 |
T9960 |
T9957 |
punct |
;,SP |
R4888 |
T9961 |
T9957 |
appos |
Leica,SP |
R4889 |
T9962 |
T9961 |
punct |
", ",Leica |
R4890 |
T9963 |
T9961 |
npadvmod |
Bensheim,Leica |
R4891 |
T9964 |
T9961 |
punct |
", ",Leica |
R4892 |
T9965 |
T9961 |
npadvmod |
FRG,Leica |
R4893 |
T9966 |
T9957 |
punct |
),SP |
R4894 |
T9967 |
T9948 |
punct |
.,examined |
R4897 |
T10006 |
T10007 |
compound |
Immunofluorescence,microscopy |
R4898 |
T10008 |
T10007 |
prep |
of,microscopy |
R4899 |
T10009 |
T10010 |
amod |
human,brain |
R4900 |
T10010 |
T10008 |
pobj |
brain,of |
R4901 |
T10012 |
T10013 |
det |
The,sections |
R4902 |
T10013 |
T10019 |
nsubjpass |
sections,deparaffinzed |
R4903 |
T10014 |
T10015 |
npadvmod |
paraffin,embedded |
R4904 |
T10015 |
T10013 |
amod |
embedded,sections |
R4905 |
T10016 |
T10015 |
punct |
-,embedded |
R4906 |
T10017 |
T10018 |
amod |
human,brain |
R4907 |
T10018 |
T10013 |
compound |
brain,sections |
R4908 |
T10020 |
T10019 |
auxpass |
were,deparaffinzed |
R4909 |
T10021 |
T10019 |
advmod |
likewise,deparaffinzed |
R4910 |
T10022 |
T10019 |
punct |
.,deparaffinzed |
R4911 |
T10024 |
T10025 |
nsubjpass |
Immunostaining,performed |
R4912 |
T10026 |
T10024 |
prep |
for,Immunostaining |
R4913 |
T10027 |
T10028 |
compound |
Annexin,A7 |
R4914 |
T10028 |
T10026 |
pobj |
A7,for |
R4915 |
T10029 |
T10025 |
auxpass |
was,performed |
R4916 |
T10030 |
T10025 |
advcl |
using,performed |
R4917 |
T10031 |
T10032 |
det |
the,antibody |
R4918 |
T10032 |
T10030 |
dobj |
antibody,using |
R4919 |
T10033 |
T10032 |
amod |
monoclonal,antibody |
R4920 |
T10034 |
T10035 |
nmod |
mAb,203 |
R4921 |
T10035 |
T10032 |
appos |
203,antibody |
R4922 |
T10036 |
T10037 |
punct |
–,217 |
R4923 |
T10037 |
T10035 |
prep |
217,203 |
R4924 |
T10038 |
T10025 |
punct |
.,performed |
R4925 |
T10040 |
T10041 |
compound |
Microwave,pre-treatment |
R4926 |
T10041 |
T10042 |
nsubjpass |
pre-treatment,used |
R4927 |
T10043 |
T10042 |
auxpass |
was,used |
R4928 |
T10044 |
T10042 |
prep |
for,used |
R4929 |
T10045 |
T10046 |
det |
all,sections |
R4930 |
T10046 |
T10044 |
pobj |
sections,for |
R4931 |
T10047 |
T10048 |
punct |
[,15 |
R4932 |
T10048 |
T10042 |
parataxis |
15,used |
R4933 |
T10049 |
T10048 |
punct |
],15 |
R4934 |
T10050 |
T10042 |
punct |
.,used |
R4935 |
T10052 |
T10053 |
compound |
Trypsin,pre-treatment |
R4936 |
T10053 |
T10054 |
nsubjpass |
pre-treatment,used |
R4937 |
T10055 |
T10054 |
auxpass |
was,used |
R4938 |
T10056 |
T10057 |
mark |
as,indicated |
R4939 |
T10057 |
T10054 |
advcl |
indicated,used |
R4940 |
T10058 |
T10054 |
punct |
.,used |
R4941 |
T10060 |
T10061 |
nmod |
Carbocyanine,2 |
R4942 |
T10061 |
T10062 |
npadvmod |
2,labeled |
R4943 |
T10062 |
T10065 |
amod |
labeled,antibodies |
R4944 |
T10063 |
T10061 |
cc |
and,2 |
R4945 |
T10064 |
T10061 |
conj |
3,2 |
R4946 |
T10065 |
T10069 |
nsubjpass |
antibodies,used |
R4947 |
T10066 |
T10065 |
amod |
anti-mouse,antibodies |
R4948 |
T10067 |
T10065 |
nmod |
IgG,antibodies |
R4949 |
T10068 |
T10065 |
amod |
secondary,antibodies |
R4950 |
T10070 |
T10071 |
punct |
(,Dianova |
R4951 |
T10071 |
T10065 |
parataxis |
Dianova,antibodies |
R4952 |
T10072 |
T10071 |
punct |
", ",Dianova |
R4953 |
T10073 |
T10071 |
npadvmod |
Hamburg,Dianova |
R4954 |
T10074 |
T10071 |
punct |
", ",Dianova |
R4955 |
T10075 |
T10071 |
npadvmod |
Germany,Dianova |
R4956 |
T10076 |
T10071 |
punct |
),Dianova |
R4957 |
T10077 |
T10069 |
auxpass |
were,used |
R4958 |
T10078 |
T10079 |
aux |
to,detect |
R4959 |
T10079 |
T10069 |
advcl |
detect,used |
R4960 |
T10080 |
T10081 |
det |
the,antibody |
R4961 |
T10081 |
T10079 |
dobj |
antibody,detect |
R4962 |
T10082 |
T10081 |
amod |
primary,antibody |
R4963 |
T10083 |
T10069 |
cc |
and,used |
R4964 |
T10084 |
T10069 |
conj |
showed,used |
R4965 |
T10085 |
T10086 |
amod |
similar,patterns |
R4966 |
T10086 |
T10084 |
dobj |
patterns,showed |
R4967 |
T10087 |
T10086 |
compound |
staining,patterns |
R4968 |
T10088 |
T10069 |
punct |
.,used |
R4969 |
T10090 |
T10091 |
amod |
Blank,controls |
R4970 |
T10091 |
T10092 |
nsubjpass |
controls,performed |
R4971 |
T10093 |
T10092 |
auxpass |
were,performed |
R4972 |
T10094 |
T10092 |
punct |
", ",performed |
R4973 |
T10095 |
T10092 |
advmod |
too,performed |
R4974 |
T10096 |
T10092 |
punct |
.,performed |
R4975 |
T10126 |
T10127 |
nmod |
Cell,culture |
R4976 |
T10127 |
T10128 |
nmod |
culture,conditions |
R4977 |
T10129 |
T10127 |
cc |
and,culture |
R4978 |
T10130 |
T10127 |
conj |
growth,culture |
R4979 |
T10132 |
T10133 |
det |
The,lines |
R4980 |
T10133 |
T10135 |
nsubjpass |
lines,cultured |
R4981 |
T10134 |
T10133 |
compound |
cell,lines |
R4982 |
T10136 |
T10133 |
acl |
used,lines |
R4983 |
T10137 |
T10135 |
auxpass |
were,cultured |
R4984 |
T10138 |
T10135 |
prep |
according,cultured |
R4985 |
T10139 |
T10138 |
prep |
to,according |
R4986 |
T10140 |
T10141 |
det |
the,instructions |
R4987 |
T10141 |
T10139 |
pobj |
instructions,to |
R4988 |
T10142 |
T10141 |
acl |
provided,instructions |
R4989 |
T10143 |
T10142 |
agent |
by,provided |
R4990 |
T10144 |
T10143 |
pobj |
ATCC,by |
R4991 |
T10145 |
T10135 |
punct |
: ,cultured |
R4992 |
T10146 |
T10147 |
amod |
murine,neuro |
R4993 |
T10147 |
T10135 |
dep |
neuro,cultured |
R4994 |
T10148 |
T10147 |
compound |
neuroblastoma,neuro |
R4995 |
T10149 |
T10147 |
punct |
-,neuro |
R4996 |
T10150 |
T10147 |
nummod |
2a,neuro |
R4997 |
T10151 |
T10152 |
punct |
(,CCL |
R4998 |
T10152 |
T10147 |
parataxis |
CCL,neuro |
R4999 |
T10153 |
T10152 |
compound |
ATCC,CCL |
R5000 |
T10154 |
T10152 |
punct |
-,CCL |
R5001 |
T10155 |
T10152 |
nummod |
131,CCL |
R5002 |
T10156 |
T10152 |
punct |
),CCL |
R5003 |
T10157 |
T10147 |
punct |
", ",neuro |
R5004 |
T10158 |
T10159 |
compound |
rat,PC |
R5005 |
T10159 |
T10147 |
conj |
PC,neuro |
R5006 |
T10160 |
T10159 |
compound |
pheochromocytoma,PC |
R5007 |
T10161 |
T10159 |
punct |
-,PC |
R5008 |
T10162 |
T10159 |
nummod |
12,PC |
R5009 |
T10163 |
T10164 |
punct |
(,CRL |
R5010 |
T10164 |
T10159 |
parataxis |
CRL,PC |
R5011 |
T10165 |
T10164 |
compound |
ATCC,CRL |
R5012 |
T10166 |
T10164 |
punct |
-,CRL |
R5013 |
T10167 |
T10164 |
nummod |
1721,CRL |
R5014 |
T10168 |
T10164 |
punct |
),CRL |
R5015 |
T10169 |
T10159 |
punct |
", ",PC |
R5016 |
T10170 |
T10159 |
cc |
and,PC |
R5017 |
T10171 |
T10172 |
compound |
rat,C6 |
R5018 |
T10172 |
T10159 |
conj |
C6,PC |
R5019 |
T10173 |
T10172 |
compound |
glioblastoma,C6 |
R5020 |
T10174 |
T10175 |
punct |
(,CCL |
R5021 |
T10175 |
T10172 |
parataxis |
CCL,C6 |
R5022 |
T10176 |
T10175 |
compound |
ATCC,CCL |
R5023 |
T10177 |
T10175 |
punct |
-,CCL |
R5024 |
T10178 |
T10175 |
nummod |
107,CCL |
R5025 |
T10179 |
T10175 |
punct |
),CCL |
R5026 |
T10180 |
T10135 |
punct |
.,cultured |
R5027 |
T10182 |
T10183 |
nummod |
One,day |
R5028 |
T10183 |
T10184 |
npadvmod |
day,before |
R5029 |
T10184 |
T10185 |
prep |
before,seeded |
R5030 |
T10186 |
T10184 |
pobj |
fixation,before |
R5031 |
T10187 |
T10186 |
prep |
with,fixation |
R5032 |
T10188 |
T10189 |
nummod |
4,% |
R5033 |
T10189 |
T10190 |
compound |
%,paraformaldehyde |
R5034 |
T10190 |
T10187 |
pobj |
paraformaldehyde,with |
R5035 |
T10191 |
T10186 |
cc |
and,fixation |
R5036 |
T10192 |
T10186 |
conj |
permeabilization,fixation |
R5037 |
T10193 |
T10192 |
prep |
with,permeabilization |
R5038 |
T10194 |
T10195 |
nummod |
0.5,% |
R5039 |
T10195 |
T10196 |
compound |
%,X |
R5040 |
T10196 |
T10193 |
pobj |
X,with |
R5041 |
T10197 |
T10196 |
compound |
Triton,X |
R5042 |
T10198 |
T10196 |
punct |
-,X |
R5043 |
T10199 |
T10196 |
nummod |
100,X |
R5044 |
T10200 |
T10185 |
punct |
", ",seeded |
R5045 |
T10201 |
T10185 |
nsubjpass |
cells,seeded |
R5046 |
T10202 |
T10185 |
auxpass |
were,seeded |
R5047 |
T10203 |
T10185 |
prep |
on,seeded |
R5048 |
T10204 |
T10203 |
pobj |
coverslips,on |
R5049 |
T10205 |
T10185 |
punct |
.,seeded |
R5050 |
T10207 |
T10208 |
nsubjpass |
Immunostaining,performed |
R5051 |
T10209 |
T10208 |
auxpass |
was,performed |
R5052 |
T10210 |
T10211 |
mark |
as,described |
R5053 |
T10211 |
T10208 |
advcl |
described,performed |
R5054 |
T10212 |
T10208 |
prep |
for,performed |
R5055 |
T10213 |
T10214 |
det |
the,sections |
R5056 |
T10214 |
T10212 |
pobj |
sections,for |
R5057 |
T10215 |
T10214 |
compound |
tissue,sections |
R5058 |
T10216 |
T10208 |
prep |
with,performed |
R5059 |
T10217 |
T10216 |
cc |
or,with |
R5060 |
T10218 |
T10216 |
conj |
without,with |
R5061 |
T10219 |
T10220 |
det |
the,treatment |
R5062 |
T10220 |
T10218 |
pobj |
treatment,without |
R5063 |
T10221 |
T10220 |
compound |
trypsin,treatment |
R5064 |
T10222 |
T10208 |
punct |
.,performed |
R5065 |
T10540 |
T10539 |
prep |
of,Extraction |
R5066 |
T10541 |
T10542 |
amod |
nuclear,A7 |
R5067 |
T10542 |
T10540 |
pobj |
A7,of |
R5068 |
T10543 |
T10542 |
compound |
Annexin,A7 |
R5069 |
T10545 |
T10546 |
amod |
Cultured,cells |
R5070 |
T10546 |
T10547 |
nsubjpass |
cells,trypsinized |
R5071 |
T10548 |
T10547 |
auxpass |
were,trypsinized |
R5072 |
T10549 |
T10547 |
punct |
", ",trypsinized |
R5073 |
T10550 |
T10547 |
conj |
counted,trypsinized |
R5074 |
T10551 |
T10550 |
cc |
and,counted |
R5075 |
T10552 |
T10550 |
conj |
washed,counted |
R5076 |
T10553 |
T10552 |
advmod |
once,washed |
R5077 |
T10554 |
T10552 |
prep |
in,washed |
R5078 |
T10555 |
T10554 |
pobj |
PBS,in |
R5079 |
T10556 |
T10547 |
punct |
.,trypsinized |
R5080 |
T10558 |
T10559 |
nsubjpass |
They,resuspended |
R5081 |
T10560 |
T10559 |
auxpass |
were,resuspended |
R5082 |
T10561 |
T10559 |
prep |
in,resuspended |
R5083 |
T10562 |
T10561 |
pobj |
buffer1,in |
R5084 |
T10563 |
T10564 |
punct |
(,sucrose |
R5085 |
T10564 |
T10562 |
parataxis |
sucrose,buffer1 |
R5086 |
T10565 |
T10566 |
nummod |
0.32,M |
R5087 |
T10566 |
T10564 |
compound |
M,sucrose |
R5088 |
T10567 |
T10564 |
punct |
", ",sucrose |
R5089 |
T10568 |
T10569 |
nummod |
10,mM |
R5090 |
T10569 |
T10570 |
compound |
mM,Tris |
R5091 |
T10570 |
T10564 |
appos |
Tris,sucrose |
R5092 |
T10571 |
T10570 |
punct |
", ",Tris |
R5093 |
T10572 |
T10570 |
npadvmod |
pH8.0,Tris |
R5094 |
T10573 |
T10564 |
punct |
", ",sucrose |
R5095 |
T10574 |
T10575 |
nummod |
3,mM |
R5096 |
T10575 |
T10576 |
compound |
mM,CaCl2 |
R5097 |
T10576 |
T10564 |
appos |
CaCl2,sucrose |
R5098 |
T10577 |
T10564 |
punct |
", ",sucrose |
R5099 |
T10578 |
T10579 |
nummod |
2,mM |
R5100 |
T10579 |
T10580 |
compound |
mM,acetate |
R5101 |
T10580 |
T10564 |
appos |
acetate,sucrose |
R5102 |
T10581 |
T10580 |
compound |
Mg,acetate |
R5103 |
T10582 |
T10580 |
punct |
-,acetate |
R5104 |
T10583 |
T10564 |
punct |
", ",sucrose |
R5105 |
T10584 |
T10585 |
nummod |
0.1,mM |
R5106 |
T10585 |
T10586 |
compound |
mM,EDTA |
R5107 |
T10586 |
T10564 |
appos |
EDTA,sucrose |
R5108 |
T10587 |
T10564 |
punct |
", ",sucrose |
R5109 |
T10588 |
T10589 |
nummod |
0.5,% |
R5110 |
T10589 |
T10590 |
compound |
%,NP40 |
R5111 |
T10590 |
T10564 |
appos |
NP40,sucrose |
R5112 |
T10591 |
T10564 |
punct |
", ",sucrose |
R5113 |
T10592 |
T10593 |
nummod |
1,mM |
R5114 |
T10593 |
T10594 |
compound |
mM,DTT |
R5115 |
T10594 |
T10564 |
appos |
DTT,sucrose |
R5116 |
T10595 |
T10564 |
punct |
", ",sucrose |
R5117 |
T10596 |
T10597 |
nummod |
0.5,mM |
R5118 |
T10597 |
T10598 |
compound |
mM,PMSF |
R5119 |
T10598 |
T10564 |
appos |
PMSF,sucrose |
R5120 |
T10599 |
T10564 |
punct |
;,sucrose |
R5121 |
T10600 |
T10601 |
nummod |
1,107cells |
R5122 |
T10601 |
T10564 |
appos |
107cells,sucrose |
R5123 |
T10602 |
T10600 |
punct |
×,1 |
R5124 |
T10603 |
T10604 |
punct |
/,buffer |
R5125 |
T10604 |
T10601 |
prep |
buffer,107cells |
R5126 |
T10605 |
T10604 |
compound |
400μl,buffer |
R5127 |
T10606 |
T10564 |
punct |
),sucrose |
R5128 |
T10607 |
T10559 |
cc |
and,resuspended |
R5129 |
T10608 |
T10559 |
conj |
sonified,resuspended |
R5130 |
T10609 |
T10608 |
prep |
on,sonified |
R5131 |
T10610 |
T10609 |
pobj |
ice,on |
R5132 |
T10611 |
T10612 |
punct |
(,cycle |
R5133 |
T10612 |
T10608 |
parataxis |
cycle,sonified |
R5134 |
T10613 |
T10614 |
compound |
sonifier,UP200S |
R5135 |
T10614 |
T10612 |
dep |
UP200S,cycle |
R5136 |
T10615 |
T10612 |
punct |
", ",cycle |
R5137 |
T10616 |
T10612 |
dep |
dr.hielscher,cycle |
R5138 |
T10617 |
T10612 |
punct |
", ",cycle |
R5139 |
T10618 |
T10619 |
nummod |
40,s |
R5140 |
T10619 |
T10612 |
dep |
s,cycle |
R5141 |
T10620 |
T10612 |
punct |
", ",cycle |
R5142 |
T10621 |
T10622 |
dep |
amplitude,% |
R5143 |
T10622 |
T10612 |
dep |
%,cycle |
R5144 |
T10623 |
T10622 |
nummod |
50,% |
R5145 |
T10624 |
T10612 |
punct |
", ",cycle |
R5146 |
T10625 |
T10612 |
nummod |
0.5,cycle |
R5147 |
T10626 |
T10612 |
punct |
),cycle |
R5148 |
T10627 |
T10559 |
punct |
.,resuspended |
R5149 |
T10629 |
T10630 |
det |
This,step |
R5150 |
T10630 |
T10631 |
nsubjpass |
step,repeated |
R5151 |
T10632 |
T10631 |
auxpass |
was,repeated |
R5152 |
T10633 |
T10634 |
mark |
until,observed |
R5153 |
T10634 |
T10631 |
advcl |
observed,repeated |
R5154 |
T10635 |
T10636 |
det |
no,cells |
R5155 |
T10636 |
T10634 |
nsubjpass |
cells,observed |
R5156 |
T10637 |
T10636 |
amod |
more,cells |
R5157 |
T10638 |
T10636 |
amod |
intact,cells |
R5158 |
T10639 |
T10634 |
auxpass |
were,observed |
R5159 |
T10640 |
T10634 |
prep |
by,observed |
R5160 |
T10641 |
T10642 |
compound |
light,microscopy |
R5161 |
T10642 |
T10640 |
pobj |
microscopy,by |
R5162 |
T10643 |
T10631 |
punct |
.,repeated |
R5163 |
T10645 |
T10646 |
det |
The,homogenate |
R5164 |
T10646 |
T10648 |
nsubjpass |
homogenate,centrifuged |
R5165 |
T10647 |
T10646 |
amod |
resulting,homogenate |
R5166 |
T10649 |
T10648 |
auxpass |
was,centrifuged |
R5167 |
T10650 |
T10648 |
prep |
at,centrifuged |
R5168 |
T10651 |
T10652 |
nummod |
500,g |
R5169 |
T10652 |
T10650 |
pobj |
g,at |
R5170 |
T10653 |
T10648 |
prep |
for,centrifuged |
R5171 |
T10654 |
T10655 |
nummod |
5,minutes |
R5172 |
T10655 |
T10653 |
pobj |
minutes,for |
R5173 |
T10656 |
T10648 |
punct |
", ",centrifuged |
R5174 |
T10657 |
T10648 |
conj |
washed,centrifuged |
R5175 |
T10658 |
T10657 |
advmod |
twice,washed |
R5176 |
T10659 |
T10657 |
prep |
in,washed |
R5177 |
T10660 |
T10661 |
nummod |
1,ml |
R5178 |
T10661 |
T10662 |
compound |
ml,buffer |
R5179 |
T10662 |
T10659 |
pobj |
buffer,in |
R5180 |
T10663 |
T10662 |
nummod |
1,buffer |
R5181 |
T10664 |
T10657 |
prep |
without,washed |
R5182 |
T10665 |
T10664 |
pobj |
NP40,without |
R5183 |
T10666 |
T10657 |
cc |
and,washed |
R5184 |
T10667 |
T10657 |
conj |
centrifuged,washed |
R5185 |
T10668 |
T10667 |
advmod |
again,centrifuged |
R5186 |
T10669 |
T10648 |
punct |
.,centrifuged |
R5187 |
T10671 |
T10672 |
det |
The,pellet |
R5188 |
T10672 |
T10674 |
nsubj |
pellet,contains |
R5189 |
T10673 |
T10672 |
amod |
resulting,pellet |
R5190 |
T10675 |
T10676 |
amod |
purified,nuclei |
R5191 |
T10676 |
T10674 |
dobj |
nuclei,contains |
R5192 |
T10677 |
T10674 |
punct |
.,contains |
R5193 |
T10679 |
T10680 |
prep |
For,resuspended |
R5194 |
T10681 |
T10682 |
amod |
partial,extraction |
R5195 |
T10682 |
T10679 |
pobj |
extraction,For |
R5196 |
T10683 |
T10682 |
prep |
of,extraction |
R5197 |
T10684 |
T10685 |
det |
the,nucleoplasm |
R5198 |
T10685 |
T10683 |
pobj |
nucleoplasm,of |
R5199 |
T10686 |
T10682 |
prep |
according,extraction |
R5200 |
T10687 |
T10686 |
prep |
to,according |
R5201 |
T10688 |
T10687 |
punct |
[,to |
R5202 |
T10689 |
T10687 |
pobj |
37,to |
R5203 |
T10690 |
T10680 |
punct |
],resuspended |
R5204 |
T10691 |
T10680 |
punct |
", ",resuspended |
R5205 |
T10692 |
T10680 |
nsubjpass |
nuclei,resuspended |
R5206 |
T10693 |
T10680 |
auxpass |
were,resuspended |
R5207 |
T10694 |
T10680 |
prep |
in,resuspended |
R5208 |
T10695 |
T10694 |
pobj |
buffer,in |
R5209 |
T10696 |
T10695 |
nummod |
2,buffer |
R5210 |
T10697 |
T10698 |
punct |
(,Hepes |
R5211 |
T10698 |
T10695 |
parataxis |
Hepes,buffer |
R5212 |
T10699 |
T10700 |
nummod |
20,mM |
R5213 |
T10700 |
T10698 |
compound |
mM,Hepes |
R5214 |
T10701 |
T10698 |
punct |
", ",Hepes |
R5215 |
T10702 |
T10698 |
npadvmod |
pH,Hepes |
R5216 |
T10703 |
T10702 |
nummod |
7.9,pH |
R5217 |
T10704 |
T10698 |
punct |
", ",Hepes |
R5218 |
T10705 |
T10706 |
nummod |
1.5,mM |
R5219 |
T10706 |
T10707 |
compound |
mM,MgCl2 |
R5220 |
T10707 |
T10698 |
appos |
MgCl2,Hepes |
R5221 |
T10708 |
T10698 |
punct |
", ",Hepes |
R5222 |
T10709 |
T10710 |
nummod |
20,mM |
R5223 |
T10710 |
T10711 |
compound |
mM,KCl |
R5224 |
T10711 |
T10698 |
appos |
KCl,Hepes |
R5225 |
T10712 |
T10698 |
punct |
", ",Hepes |
R5226 |
T10713 |
T10714 |
nummod |
0.2,mM |
R5227 |
T10714 |
T10715 |
compound |
mM,EDTA |
R5228 |
T10715 |
T10698 |
appos |
EDTA,Hepes |
R5229 |
T10716 |
T10698 |
punct |
", ",Hepes |
R5230 |
T10717 |
T10718 |
nummod |
25,% |
R5231 |
T10718 |
T10719 |
nmod |
%,glycerine |
R5232 |
T10719 |
T10698 |
appos |
glycerine,Hepes |
R5233 |
T10720 |
T10718 |
appos |
v,% |
R5234 |
T10721 |
T10722 |
punct |
/,v |
R5235 |
T10722 |
T10720 |
prep |
v,v |
R5236 |
T10723 |
T10698 |
punct |
", ",Hepes |
R5237 |
T10724 |
T10725 |
nummod |
0.5,mM |
R5238 |
T10725 |
T10726 |
compound |
mM,DTT |
R5239 |
T10726 |
T10698 |
appos |
DTT,Hepes |
R5240 |
T10727 |
T10698 |
punct |
", ",Hepes |
R5241 |
T10728 |
T10729 |
nummod |
0.5,mM |
R5242 |
T10729 |
T10730 |
compound |
mM,PMSF |
R5243 |
T10730 |
T10698 |
appos |
PMSF,Hepes |
R5244 |
T10731 |
T10698 |
punct |
;,Hepes |
R5245 |
T10732 |
T10698 |
appos |
nuclei,Hepes |
R5246 |
T10733 |
T10732 |
prep |
of,nuclei |
R5247 |
T10734 |
T10735 |
nmod |
107cells,buffer |
R5248 |
T10735 |
T10733 |
pobj |
buffer,of |
R5249 |
T10736 |
T10737 |
punct |
/,μl |
R5250 |
T10737 |
T10734 |
prep |
μl,107cells |
R5251 |
T10738 |
T10737 |
nummod |
30,μl |
R5252 |
T10739 |
T10698 |
punct |
),Hepes |
R5253 |
T10740 |
T10680 |
cc |
and,resuspended |
R5254 |
T10741 |
T10680 |
conj |
incubated,resuspended |
R5255 |
T10742 |
T10741 |
prep |
on,incubated |
R5256 |
T10743 |
T10742 |
pobj |
ice,on |
R5257 |
T10744 |
T10741 |
prep |
for,incubated |
R5258 |
T10745 |
T10746 |
nummod |
30,minutes |
R5259 |
T10746 |
T10744 |
pobj |
minutes,for |
R5260 |
T10747 |
T10680 |
punct |
.,resuspended |
R5261 |
T10749 |
T10750 |
det |
An,volume |
R5262 |
T10750 |
T10752 |
nsubjpass |
volume,added |
R5263 |
T10751 |
T10750 |
amod |
equal,volume |
R5264 |
T10753 |
T10750 |
prep |
of,volume |
R5265 |
T10754 |
T10753 |
pobj |
buffer,of |
R5266 |
T10755 |
T10754 |
nummod |
3,buffer |
R5267 |
T10756 |
T10752 |
auxpass |
was,added |
R5268 |
T10757 |
T10752 |
prep |
in,added |
R5269 |
T10758 |
T10757 |
pobj |
aliquots,in |
R5270 |
T10759 |
T10760 |
punct |
(,Sigma |
R5271 |
T10760 |
T10752 |
parataxis |
Sigma,added |
R5272 |
T10761 |
T10762 |
nummod |
20,mM |
R5273 |
T10762 |
T10763 |
compound |
mM,Hepes |
R5274 |
T10763 |
T10760 |
dep |
Hepes,Sigma |
R5275 |
T10764 |
T10763 |
punct |
", ",Hepes |
R5276 |
T10765 |
T10763 |
npadvmod |
pH,Hepes |
R5277 |
T10766 |
T10765 |
nummod |
7.9,pH |
R5278 |
T10767 |
T10763 |
punct |
", ",Hepes |
R5279 |
T10768 |
T10769 |
nummod |
1.5,mM |
R5280 |
T10769 |
T10770 |
compound |
mM,MgCl2 |
R5281 |
T10770 |
T10763 |
appos |
MgCl2,Hepes |
R5282 |
T10771 |
T10763 |
punct |
", ",Hepes |
R5283 |
T10772 |
T10773 |
nummod |
800,mM |
R5284 |
T10773 |
T10774 |
compound |
mM,KCl |
R5285 |
T10774 |
T10763 |
appos |
KCl,Hepes |
R5286 |
T10775 |
T10763 |
punct |
", ",Hepes |
R5287 |
T10776 |
T10777 |
nummod |
0.2,mM |
R5288 |
T10777 |
T10778 |
compound |
mM,EDTA |
R5289 |
T10778 |
T10763 |
appos |
EDTA,Hepes |
R5290 |
T10779 |
T10763 |
punct |
", ",Hepes |
R5291 |
T10780 |
T10781 |
nummod |
25,% |
R5292 |
T10781 |
T10782 |
nmod |
%,glycerine |
R5293 |
T10782 |
T10763 |
appos |
glycerine,Hepes |
R5294 |
T10783 |
T10781 |
appos |
v,% |
R5295 |
T10784 |
T10785 |
punct |
/,v |
R5296 |
T10785 |
T10783 |
prep |
v,v |
R5297 |
T10786 |
T10763 |
punct |
", ",Hepes |
R5298 |
T10787 |
T10788 |
nummod |
0.5,mM |
R5299 |
T10788 |
T10789 |
compound |
mM,DTT |
R5300 |
T10789 |
T10763 |
appos |
DTT,Hepes |
R5301 |
T10790 |
T10763 |
punct |
", ",Hepes |
R5302 |
T10791 |
T10792 |
nummod |
0.5,mM |
R5303 |
T10792 |
T10793 |
compound |
mM,PMSF |
R5304 |
T10793 |
T10763 |
appos |
PMSF,Hepes |
R5305 |
T10794 |
T10763 |
punct |
", ",Hepes |
R5306 |
T10795 |
T10796 |
nummod |
1,% |
R5307 |
T10796 |
T10797 |
compound |
%,NP40 |
R5308 |
T10797 |
T10763 |
appos |
NP40,Hepes |
R5309 |
T10798 |
T10763 |
punct |
", ",Hepes |
R5310 |
T10799 |
T10800 |
compound |
protease,inhibitor |
R5311 |
T10800 |
T10801 |
compound |
inhibitor,cocktail |
R5312 |
T10801 |
T10763 |
appos |
cocktail,Hepes |
R5313 |
T10802 |
T10760 |
punct |
(,Sigma |
R5314 |
T10803 |
T10760 |
punct |
),Sigma |
R5315 |
T10804 |
T10760 |
punct |
),Sigma |
R5316 |
T10805 |
T10752 |
cc |
and,added |
R5317 |
T10806 |
T10807 |
det |
the,solution |
R5318 |
T10807 |
T10808 |
nsubjpass |
solution,incubated |
R5319 |
T10808 |
T10752 |
conj |
incubated,added |
R5320 |
T10809 |
T10808 |
auxpass |
was,incubated |
R5321 |
T10810 |
T10811 |
mark |
while,shaking |
R5322 |
T10811 |
T10808 |
advcl |
shaking,incubated |
R5323 |
T10812 |
T10811 |
prep |
for,shaking |
R5324 |
T10813 |
T10814 |
amod |
further,minutes |
R5325 |
T10814 |
T10812 |
pobj |
minutes,for |
R5326 |
T10815 |
T10814 |
nummod |
30,minutes |
R5327 |
T10816 |
T10811 |
prep |
on,shaking |
R5328 |
T10817 |
T10816 |
pobj |
ice,on |
R5329 |
T10818 |
T10808 |
punct |
.,incubated |
R5330 |
T10820 |
T10821 |
advmod |
Then,centrifuged |
R5331 |
T10822 |
T10823 |
det |
the,solution |
R5332 |
T10823 |
T10821 |
nsubjpass |
solution,centrifuged |
R5333 |
T10824 |
T10821 |
auxpass |
was,centrifuged |
R5334 |
T10825 |
T10821 |
prep |
for,centrifuged |
R5335 |
T10826 |
T10827 |
nummod |
15,minutes |
R5336 |
T10827 |
T10825 |
pobj |
minutes,for |
R5337 |
T10828 |
T10821 |
prep |
at,centrifuged |
R5338 |
T10829 |
T10830 |
nummod |
14.000,g |
R5339 |
T10830 |
T10828 |
pobj |
g,at |
R5340 |
T10831 |
T10821 |
punct |
", ",centrifuged |
R5341 |
T10832 |
T10833 |
det |
the,supernatant |
R5342 |
T10833 |
T10835 |
nsubjpass |
supernatant,used |
R5343 |
T10834 |
T10833 |
amod |
resulting,supernatant |
R5344 |
T10835 |
T10821 |
conj |
used,centrifuged |
R5345 |
T10836 |
T10835 |
auxpass |
was,used |
R5346 |
T10837 |
T10835 |
prep |
as,used |
R5347 |
T10838 |
T10837 |
pobj |
nucleoplasm,as |
R5348 |
T10839 |
T10835 |
punct |
", ",used |
R5349 |
T10840 |
T10835 |
cc |
and,used |
R5350 |
T10841 |
T10842 |
det |
the,pellet |
R5351 |
T10842 |
T10843 |
nsubjpass |
pellet,washed |
R5352 |
T10843 |
T10835 |
conj |
washed,used |
R5353 |
T10844 |
T10843 |
auxpass |
was,washed |
R5354 |
T10845 |
T10843 |
advmod |
once,washed |
R5355 |
T10846 |
T10843 |
cc |
and,washed |
R5356 |
T10847 |
T10843 |
conj |
adjusted,washed |
R5357 |
T10848 |
T10847 |
prep |
to,adjusted |
R5358 |
T10849 |
T10850 |
det |
an,volume |
R5359 |
T10850 |
T10848 |
pobj |
volume,to |
R5360 |
T10851 |
T10850 |
amod |
equal,volume |
R5361 |
T10852 |
T10847 |
prep |
with,adjusted |
R5362 |
T10853 |
T10852 |
pobj |
PBS,with |
R5363 |
T10854 |
T10843 |
punct |
.,washed |
R5364 |
T10856 |
T10857 |
nsubjpass |
Nucleoplasm,subjected |
R5365 |
T10858 |
T10856 |
cc |
and,Nucleoplasm |
R5366 |
T10859 |
T10860 |
amod |
nuclear,pellet |
R5367 |
T10860 |
T10856 |
conj |
pellet,Nucleoplasm |
R5368 |
T10861 |
T10857 |
auxpass |
were,subjected |
R5369 |
T10862 |
T10857 |
prep |
to,subjected |
R5370 |
T10863 |
T10864 |
compound |
SDS,PAGE |
R5371 |
T10864 |
T10862 |
pobj |
PAGE,to |
R5372 |
T10865 |
T10864 |
punct |
-,PAGE |
R5373 |
T10866 |
T10864 |
cc |
and,PAGE |
R5374 |
T10867 |
T10868 |
compound |
Western,blotting |
R5375 |
T10868 |
T10864 |
conj |
blotting,PAGE |
R5376 |
T10869 |
T10857 |
punct |
.,subjected |
R5377 |
T10896 |
T10897 |
compound |
Western,blotting |
R5378 |
T10899 |
T10900 |
nsubjpass |
Cells,lysed |
R5379 |
T10901 |
T10899 |
cc |
or,Cells |
R5380 |
T10902 |
T10903 |
amod |
cellular,fractions |
R5381 |
T10903 |
T10899 |
conj |
fractions,Cells |
R5382 |
T10904 |
T10900 |
auxpass |
were,lysed |
R5383 |
T10905 |
T10900 |
prep |
in,lysed |
R5384 |
T10906 |
T10907 |
compound |
SDS,buffer |
R5385 |
T10907 |
T10905 |
pobj |
buffer,in |
R5386 |
T10908 |
T10907 |
compound |
sample,buffer |
R5387 |
T10909 |
T10900 |
cc |
and,lysed |
R5388 |
T10910 |
T10911 |
nsubjpass |
homogenates,subjected |
R5389 |
T10911 |
T10900 |
conj |
subjected,lysed |
R5390 |
T10912 |
T10911 |
auxpass |
were,subjected |
R5391 |
T10913 |
T10911 |
prep |
to,subjected |
R5392 |
T10914 |
T10915 |
compound |
SDS,PAGE |
R5393 |
T10915 |
T10913 |
pobj |
PAGE,to |
R5394 |
T10916 |
T10915 |
punct |
-,PAGE |
R5395 |
T10917 |
T10911 |
punct |
.,subjected |
R5396 |
T10919 |
T10920 |
compound |
Western,blotting |
R5397 |
T10920 |
T10921 |
nsubjpass |
blotting,carried |
R5398 |
T10922 |
T10921 |
auxpass |
was,carried |
R5399 |
T10923 |
T10921 |
prt |
out,carried |
R5400 |
T10924 |
T10921 |
advcl |
using,carried |
R5401 |
T10925 |
T10926 |
det |
the,method |
R5402 |
T10926 |
T10924 |
dobj |
method,using |
R5403 |
T10927 |
T10926 |
prep |
of,method |
R5404 |
T10928 |
T10927 |
pobj |
Towbin,of |
R5405 |
T10929 |
T10930 |
advmod |
et,al. |
R5406 |
T10930 |
T10928 |
advmod |
al.,Towbin |
R5407 |
T10931 |
T10932 |
punct |
[,38 |
R5408 |
T10932 |
T10921 |
parataxis |
38,carried |
R5409 |
T10933 |
T10932 |
punct |
],38 |
R5410 |
T10934 |
T10921 |
punct |
.,carried |
R5411 |
T10936 |
T10937 |
npadvmod |
Peroxidase,coupled |
R5412 |
T10937 |
T10939 |
amod |
coupled,antibodies |
R5413 |
T10938 |
T10937 |
punct |
-,coupled |
R5414 |
T10939 |
T10941 |
nsubjpass |
antibodies,used |
R5415 |
T10940 |
T10939 |
amod |
secondary,antibodies |
R5416 |
T10942 |
T10943 |
punct |
(,Sigma |
R5417 |
T10943 |
T10939 |
parataxis |
Sigma,antibodies |
R5418 |
T10944 |
T10943 |
punct |
),Sigma |
R5419 |
T10945 |
T10939 |
cc |
and,antibodies |
R5420 |
T10946 |
T10947 |
det |
the,system |
R5421 |
T10947 |
T10939 |
conj |
system,antibodies |
R5422 |
T10948 |
T10949 |
npadvmod |
supersignal,enhanced |
R5423 |
T10949 |
T10947 |
amod |
enhanced,system |
R5424 |
T10950 |
T10947 |
compound |
chemiluminescence,system |
R5425 |
T10951 |
T10952 |
punct |
(,Pierce |
R5426 |
T10952 |
T10947 |
parataxis |
Pierce,system |
R5427 |
T10953 |
T10952 |
punct |
", ",Pierce |
R5428 |
T10954 |
T10952 |
npadvmod |
Rockford,Pierce |
R5429 |
T10955 |
T10952 |
punct |
", ",Pierce |
R5430 |
T10956 |
T10952 |
npadvmod |
IL,Pierce |
R5431 |
T10957 |
T10952 |
punct |
", ",Pierce |
R5432 |
T10958 |
T10952 |
npadvmod |
USA,Pierce |
R5433 |
T10959 |
T10952 |
punct |
),Pierce |
R5434 |
T10960 |
T10941 |
auxpass |
were,used |
R5435 |
T10961 |
T10941 |
punct |
.,used |
R5436 |
T11182 |
T11183 |
nmod |
Embryo,Blot |
R5437 |
T11184 |
T11185 |
amod |
Multiple,Tissue |
R5438 |
T11185 |
T11183 |
compound |
Tissue,Blot |
R5439 |
T11186 |
T11183 |
compound |
Northern,Blot |
R5440 |
T11188 |
T11189 |
det |
The,presence |
R5441 |
T11189 |
T11190 |
nsubjpass |
presence,analyzed |
R5442 |
T11191 |
T11189 |
prep |
of,presence |
R5443 |
T11192 |
T11193 |
compound |
Annexin,A7 |
R5444 |
T11193 |
T11194 |
compound |
A7,mRNA |
R5445 |
T11194 |
T11191 |
pobj |
mRNA,of |
R5446 |
T11195 |
T11189 |
prep |
in,presence |
R5447 |
T11196 |
T11197 |
compound |
mouse,cells |
R5448 |
T11197 |
T11195 |
pobj |
cells,in |
R5449 |
T11198 |
T11197 |
compound |
ES,cells |
R5450 |
T11199 |
T11197 |
cc |
and,cells |
R5451 |
T11200 |
T11201 |
compound |
embryos,E7 |
R5452 |
T11201 |
T11197 |
conj |
E7,cells |
R5453 |
T11202 |
T11201 |
punct |
", ",E7 |
R5454 |
T11203 |
T11201 |
conj |
E11,E7 |
R5455 |
T11204 |
T11203 |
punct |
", ",E11 |
R5456 |
T11205 |
T11203 |
conj |
E15,E11 |
R5457 |
T11206 |
T11205 |
cc |
and,E15 |
R5458 |
T11207 |
T11205 |
conj |
E17,E15 |
R5459 |
T11208 |
T11190 |
auxpass |
was,analyzed |
R5460 |
T11209 |
T11190 |
prep |
by,analyzed |
R5461 |
T11210 |
T11211 |
compound |
northern,blot |
R5462 |
T11211 |
T11212 |
compound |
blot,analysis |
R5463 |
T11212 |
T11209 |
pobj |
analysis,by |
R5464 |
T11213 |
T11190 |
punct |
.,analyzed |
R5465 |
T11215 |
T11216 |
det |
An,membrane |
R5466 |
T11216 |
T11222 |
nsubjpass |
membrane,obtained |
R5467 |
T11217 |
T11216 |
nmod |
Embryo,membrane |
R5468 |
T11218 |
T11219 |
amod |
Multiple,Tissue |
R5469 |
T11219 |
T11216 |
compound |
Tissue,membrane |
R5470 |
T11220 |
T11221 |
compound |
Northern,Blot |
R5471 |
T11221 |
T11216 |
compound |
Blot,membrane |
R5472 |
T11223 |
T11222 |
auxpass |
was,obtained |
R5473 |
T11224 |
T11222 |
prep |
from,obtained |
R5474 |
T11225 |
T11226 |
compound |
Clontech,Laboratories |
R5475 |
T11226 |
T11224 |
pobj |
Laboratories,from |
R5476 |
T11227 |
T11228 |
punct |
(,Heidelberg |
R5477 |
T11228 |
T11226 |
parataxis |
Heidelberg,Laboratories |
R5478 |
T11229 |
T11228 |
punct |
", ",Heidelberg |
R5479 |
T11230 |
T11228 |
npadvmod |
FRG,Heidelberg |
R5480 |
T11231 |
T11228 |
punct |
),Heidelberg |
R5481 |
T11232 |
T11222 |
punct |
.,obtained |
R5482 |
T11234 |
T11235 |
nsubj |
It,contained |
R5483 |
T11236 |
T11237 |
advmod |
approximately,2 |
R5484 |
T11237 |
T11238 |
nummod |
2,μg |
R5485 |
T11238 |
T11235 |
dobj |
μg,contained |
R5486 |
T11239 |
T11238 |
prep |
of,μg |
R5487 |
T11240 |
T11241 |
nmod |
poly,RNA |
R5488 |
T11241 |
T11239 |
pobj |
RNA,of |
R5489 |
T11242 |
T11243 |
punct |
(,A |
R5490 |
T11243 |
T11241 |
parataxis |
A,RNA |
R5491 |
T11244 |
T11243 |
punct |
),A |
R5492 |
T11245 |
T11241 |
punct |
+,RNA |
R5493 |
T11246 |
T11238 |
prep |
per,μg |
R5494 |
T11247 |
T11246 |
pobj |
lane,per |
R5495 |
T11248 |
T11238 |
prep |
from,μg |
R5496 |
T11249 |
T11250 |
nummod |
four,stages |
R5497 |
T11250 |
T11248 |
pobj |
stages,from |
R5498 |
T11251 |
T11250 |
amod |
different,stages |
R5499 |
T11252 |
T11250 |
prep |
of,stages |
R5500 |
T11253 |
T11254 |
compound |
mouse,development |
R5501 |
T11254 |
T11252 |
pobj |
development,of |
R5502 |
T11255 |
T11235 |
punct |
.,contained |
R5503 |
T11257 |
T11258 |
nsubjpass |
RNA,isolated |
R5504 |
T11259 |
T11257 |
prep |
from,RNA |
R5505 |
T11260 |
T11261 |
compound |
ES,cells |
R5506 |
T11261 |
T11259 |
pobj |
cells,from |
R5507 |
T11262 |
T11258 |
auxpass |
was,isolated |
R5508 |
T11263 |
T11258 |
prep |
with,isolated |
R5509 |
T11264 |
T11265 |
det |
the,kit |
R5510 |
T11265 |
T11263 |
pobj |
kit,with |
R5511 |
T11266 |
T11265 |
compound |
RNeasy,kit |
R5512 |
T11267 |
T11268 |
punct |
(,Qiagen |
R5513 |
T11268 |
T11265 |
parataxis |
Qiagen,kit |
R5514 |
T11269 |
T11268 |
punct |
),Qiagen |
R5515 |
T11270 |
T11258 |
punct |
.,isolated |
R5516 |
T11272 |
T11273 |
det |
The,membranes |
R5517 |
T11273 |
T11274 |
nsubjpass |
membranes,hybridized |
R5518 |
T11275 |
T11274 |
auxpass |
were,hybridized |
R5519 |
T11276 |
T11274 |
prep |
with,hybridized |
R5520 |
T11277 |
T11278 |
det |
a,probe |
R5521 |
T11278 |
T11276 |
pobj |
probe,with |
R5522 |
T11279 |
T11280 |
amod |
random,primed |
R5523 |
T11280 |
T11278 |
amod |
primed,probe |
R5524 |
T11281 |
T11280 |
punct |
-,primed |
R5525 |
T11282 |
T11283 |
punct |
[,α |
R5526 |
T11283 |
T11280 |
parataxis |
α,primed |
R5527 |
T11284 |
T11283 |
punct |
-,α |
R5528 |
T11285 |
T11283 |
nummod |
32P,α |
R5529 |
T11286 |
T11283 |
punct |
],α |
R5530 |
T11287 |
T11278 |
punct |
-,probe |
R5531 |
T11288 |
T11289 |
npadvmod |
dATP,labelled |
R5532 |
T11289 |
T11278 |
amod |
labelled,probe |
R5533 |
T11290 |
T11289 |
punct |
-,labelled |
R5534 |
T11291 |
T11292 |
amod |
full,length |
R5535 |
T11292 |
T11278 |
compound |
length,probe |
R5536 |
T11293 |
T11294 |
compound |
annexin,A7 |
R5537 |
T11294 |
T11278 |
compound |
A7,probe |
R5538 |
T11295 |
T11278 |
compound |
cDNA,probe |
R5539 |
T11296 |
T11276 |
cc |
and,with |
R5540 |
T11297 |
T11276 |
conj |
with,with |
R5541 |
T11298 |
T11299 |
det |
a,probe |
R5542 |
T11299 |
T11297 |
pobj |
probe,with |
R5543 |
T11300 |
T11301 |
compound |
β,actin |
R5544 |
T11301 |
T11303 |
npadvmod |
actin,specific |
R5545 |
T11302 |
T11301 |
punct |
-,actin |
R5546 |
T11303 |
T11299 |
amod |
specific,probe |
R5547 |
T11304 |
T11274 |
prep |
for,hybridized |
R5548 |
T11305 |
T11304 |
pobj |
control,for |
R5549 |
T11306 |
T11274 |
prep |
in,hybridized |
R5550 |
T11307 |
T11308 |
compound |
ExpressHyb,solution |
R5551 |
T11308 |
T11306 |
pobj |
solution,in |
R5552 |
T11309 |
T11274 |
prep |
according,hybridized |
R5553 |
T11310 |
T11309 |
prep |
to,according |
R5554 |
T11311 |
T11312 |
det |
the,manufacturer |
R5555 |
T11312 |
T11313 |
poss |
manufacturer,directions |
R5556 |
T11313 |
T11310 |
pobj |
directions,to |
R5557 |
T11314 |
T11312 |
case |
's,manufacturer |
R5558 |
T11315 |
T11274 |
punct |
.,hybridized |
R5559 |
T11655 |
T11654 |
prep |
of,Expression |
R5560 |
T11656 |
T11657 |
compound |
Annexin,A7 |
R5561 |
T11657 |
T11655 |
pobj |
A7,of |
R5562 |
T11658 |
T11654 |
punct |
.,Expression |
R5563 |
T11660 |
T11661 |
punct |
(,A |
R5564 |
T11661 |
T11662 |
meta |
A,analysis |
R5565 |
T11663 |
T11661 |
punct |
),A |
R5566 |
T11664 |
T11665 |
compound |
Northern,blot |
R5567 |
T11665 |
T11662 |
compound |
blot,analysis |
R5568 |
T11666 |
T11662 |
prep |
of,analysis |
R5569 |
T11667 |
T11666 |
pobj |
RNA,of |
R5570 |
T11668 |
T11667 |
prep |
from,RNA |
R5571 |
T11669 |
T11670 |
compound |
ES,cells |
R5572 |
T11670 |
T11668 |
pobj |
cells,from |
R5573 |
T11671 |
T11670 |
punct |
-,cells |
R5574 |
T11672 |
T11670 |
cc |
and,cells |
R5575 |
T11673 |
T11674 |
nmod |
embryos,E17 |
R5576 |
T11674 |
T11670 |
conj |
E17,cells |
R5577 |
T11675 |
T11674 |
nmod |
E7,E17 |
R5578 |
T11676 |
T11674 |
punct |
", ",E17 |
R5579 |
T11677 |
T11674 |
nmod |
E11,E17 |
R5580 |
T11678 |
T11674 |
punct |
", ",E17 |
R5581 |
T11679 |
T11674 |
nmod |
E15,E17 |
R5582 |
T11680 |
T11674 |
punct |
", ",E17 |
R5583 |
T11681 |
T11667 |
acl |
probed,RNA |
R5584 |
T11682 |
T11681 |
prep |
with,probed |
R5585 |
T11683 |
T11684 |
det |
a,cDNA |
R5586 |
T11684 |
T11682 |
pobj |
cDNA,with |
R5587 |
T11685 |
T11686 |
amod |
full,length |
R5588 |
T11686 |
T11684 |
compound |
length,cDNA |
R5589 |
T11687 |
T11688 |
compound |
annexin,A7 |
R5590 |
T11688 |
T11684 |
compound |
A7,cDNA |
R5591 |
T11689 |
T11662 |
punct |
.,analysis |
R5592 |
T11691 |
T11692 |
nsubjpass |
Transcripts,detected |
R5593 |
T11693 |
T11691 |
prep |
of,Transcripts |
R5594 |
T11694 |
T11695 |
nummod |
1.8,kb |
R5595 |
T11695 |
T11693 |
pobj |
kb,of |
R5596 |
T11696 |
T11694 |
cc |
and,1.8 |
R5597 |
T11697 |
T11694 |
conj |
2.4,1.8 |
R5598 |
T11698 |
T11692 |
auxpass |
are,detected |
R5599 |
T11699 |
T11692 |
prep |
in,detected |
R5600 |
T11700 |
T11701 |
det |
all,stages |
R5601 |
T11701 |
T11699 |
pobj |
stages,in |
R5602 |
T11702 |
T11701 |
acl |
examined,stages |
R5603 |
T11703 |
T11704 |
punct |
(,panel |
R5604 |
T11704 |
T11692 |
parataxis |
panel,detected |
R5605 |
T11705 |
T11704 |
amod |
upper,panel |
R5606 |
T11706 |
T11704 |
punct |
),panel |
R5607 |
T11707 |
T11692 |
punct |
.,detected |
R5608 |
T11709 |
T11710 |
nsubj |
Reprobing,verified |
R5609 |
T11711 |
T11709 |
prep |
with,Reprobing |
R5610 |
T11712 |
T11713 |
det |
a,probe |
R5611 |
T11713 |
T11711 |
pobj |
probe,with |
R5612 |
T11714 |
T11715 |
compound |
β,actin |
R5613 |
T11715 |
T11713 |
compound |
actin,probe |
R5614 |
T11716 |
T11715 |
punct |
-,actin |
R5615 |
T11717 |
T11718 |
amod |
equal,loading |
R5616 |
T11718 |
T11710 |
dobj |
loading,verified |
R5617 |
T11719 |
T11720 |
punct |
(,band |
R5618 |
T11720 |
T11710 |
parataxis |
band,verified |
R5619 |
T11721 |
T11722 |
amod |
lower,panel |
R5620 |
T11722 |
T11720 |
dep |
panel,band |
R5621 |
T11723 |
T11720 |
punct |
", ",band |
R5622 |
T11724 |
T11725 |
nummod |
2.0,kb |
R5623 |
T11725 |
T11720 |
compound |
kb,band |
R5624 |
T11726 |
T11720 |
punct |
),band |
R5625 |
T11727 |
T11710 |
punct |
.,verified |
R5626 |
T11729 |
T11730 |
punct |
(,B |
R5627 |
T11730 |
T11731 |
meta |
B,express |
R5628 |
T11732 |
T11730 |
punct |
),B |
R5629 |
T11733 |
T11734 |
amod |
Murine,cells |
R5630 |
T11734 |
T11731 |
nsubj |
cells,express |
R5631 |
T11735 |
T11734 |
compound |
ES,cells |
R5632 |
T11736 |
T11734 |
punct |
(,cells |
R5633 |
T11737 |
T11734 |
appos |
ES,cells |
R5634 |
T11738 |
T11731 |
punct |
),express |
R5635 |
T11739 |
T11740 |
advmod |
only,isoform |
R5636 |
T11740 |
T11731 |
dobj |
isoform,express |
R5637 |
T11741 |
T11740 |
det |
the,isoform |
R5638 |
T11742 |
T11740 |
amod |
small,isoform |
R5639 |
T11743 |
T11744 |
compound |
Annexin,A7 |
R5640 |
T11744 |
T11740 |
compound |
A7,isoform |
R5641 |
T11745 |
T11731 |
punct |
.,express |
R5642 |
T11747 |
T11748 |
det |
Both,isoforms |
R5643 |
T11748 |
T11749 |
nsubjpass |
isoforms,expressed |
R5644 |
T11750 |
T11749 |
auxpass |
are,expressed |
R5645 |
T11751 |
T11749 |
prep |
in,expressed |
R5646 |
T11752 |
T11753 |
compound |
neuroblastoma,cells |
R5647 |
T11753 |
T11751 |
pobj |
cells,in |
R5648 |
T11754 |
T11755 |
compound |
neuro,2a |
R5649 |
T11755 |
T11753 |
compound |
2a,cells |
R5650 |
T11756 |
T11755 |
punct |
-,2a |
R5651 |
T11757 |
T11753 |
punct |
(,cells |
R5652 |
T11758 |
T11753 |
appos |
N2A,cells |
R5653 |
T11759 |
T11753 |
punct |
),cells |
R5654 |
T11760 |
T11753 |
punct |
", ",cells |
R5655 |
T11761 |
T11753 |
conj |
heart,cells |
R5656 |
T11762 |
T11761 |
punct |
(,heart |
R5657 |
T11763 |
T11761 |
appos |
HRT,heart |
R5658 |
T11764 |
T11761 |
punct |
),heart |
R5659 |
T11765 |
T11761 |
cc |
and,heart |
R5660 |
T11766 |
T11767 |
compound |
brain,tissue |
R5661 |
T11767 |
T11761 |
conj |
tissue,heart |
R5662 |
T11768 |
T11767 |
punct |
(,tissue |
R5663 |
T11769 |
T11767 |
appos |
CNS,tissue |
R5664 |
T11770 |
T11753 |
punct |
),cells |
R5665 |
T11771 |
T11753 |
prep |
from,cells |
R5666 |
T11772 |
T11773 |
amod |
adult,mice |
R5667 |
T11773 |
T11771 |
pobj |
mice,from |
R5668 |
T11774 |
T11749 |
punct |
.,expressed |
R5669 |
T11776 |
T11777 |
nsubjpass |
Proteins,resolved |
R5670 |
T11778 |
T11776 |
prep |
from,Proteins |
R5671 |
T11779 |
T11780 |
compound |
neuro,2a |
R5672 |
T11780 |
T11778 |
pobj |
2a,from |
R5673 |
T11781 |
T11780 |
punct |
-,2a |
R5674 |
T11782 |
T11780 |
cc |
and,2a |
R5675 |
T11783 |
T11780 |
conj |
ES,2a |
R5676 |
T11784 |
T11780 |
appos |
cells,2a |
R5677 |
T11785 |
T11784 |
punct |
", ",cells |
R5678 |
T11786 |
T11784 |
conj |
heart,cells |
R5679 |
T11787 |
T11786 |
cc |
and,heart |
R5680 |
T11788 |
T11786 |
conj |
brain,heart |
R5681 |
T11789 |
T11777 |
auxpass |
were,resolved |
R5682 |
T11790 |
T11777 |
prep |
by,resolved |
R5683 |
T11791 |
T11792 |
compound |
SDS,electrophoresis |
R5684 |
T11792 |
T11790 |
pobj |
electrophoresis,by |
R5685 |
T11793 |
T11794 |
compound |
polyacrylamide,gel |
R5686 |
T11794 |
T11792 |
compound |
gel,electrophoresis |
R5687 |
T11795 |
T11796 |
punct |
(,acrylamide |
R5688 |
T11796 |
T11777 |
parataxis |
acrylamide,resolved |
R5689 |
T11797 |
T11798 |
nummod |
12,% |
R5690 |
T11798 |
T11796 |
compound |
%,acrylamide |
R5691 |
T11799 |
T11796 |
punct |
),acrylamide |
R5692 |
T11800 |
T11777 |
cc |
and,resolved |
R5693 |
T11801 |
T11777 |
conj |
transferred,resolved |
R5694 |
T11802 |
T11801 |
prep |
to,transferred |
R5695 |
T11803 |
T11804 |
compound |
nitrocellulose,membranes |
R5696 |
T11804 |
T11802 |
pobj |
membranes,to |
R5697 |
T11805 |
T11777 |
punct |
.,resolved |
R5698 |
T11807 |
T11808 |
det |
The,blot |
R5699 |
T11808 |
T11810 |
nsubjpass |
blot,probed |
R5700 |
T11809 |
T11808 |
compound |
Western,blot |
R5701 |
T11811 |
T11810 |
auxpass |
was,probed |
R5702 |
T11812 |
T11810 |
prep |
with,probed |
R5703 |
T11813 |
T11814 |
nmod |
mAb,203 |
R5704 |
T11814 |
T11812 |
pobj |
203,with |
R5705 |
T11815 |
T11816 |
punct |
–,217 |
R5706 |
T11816 |
T11814 |
prep |
217,203 |
R5707 |
T11817 |
T11814 |
acl |
followed,203 |
R5708 |
T11818 |
T11817 |
agent |
by,followed |
R5709 |
T11819 |
T11818 |
pobj |
incubation,by |
R5710 |
T11820 |
T11819 |
prep |
with,incubation |
R5711 |
T11821 |
T11822 |
det |
a,antibody |
R5712 |
T11822 |
T11820 |
pobj |
antibody,with |
R5713 |
T11823 |
T11824 |
npadvmod |
peroxidase,coupled |
R5714 |
T11824 |
T11822 |
amod |
coupled,antibody |
R5715 |
T11825 |
T11822 |
amod |
secondary,antibody |
R5716 |
T11826 |
T11810 |
punct |
.,probed |
R5717 |
T11828 |
T11829 |
nsubj |
Detection,was |
R5718 |
T11830 |
T11829 |
prep |
with,was |
R5719 |
T11831 |
T11832 |
amod |
enhanced,chemiluminescence |
R5720 |
T11832 |
T11830 |
pobj |
chemiluminescence,with |
R5721 |
T11833 |
T11829 |
punct |
.,was |
R5724 |
T12193 |
T12194 |
compound |
Annexin,A7 |
R5725 |
T12194 |
T12195 |
compound |
A7,immunoreactivity |
R5726 |
T12196 |
T12195 |
prep |
in,immunoreactivity |
R5727 |
T12197 |
T12198 |
amod |
early,embryos |
R5728 |
T12198 |
T12196 |
pobj |
embryos,in |
R5729 |
T12199 |
T12198 |
compound |
mouse,embryos |
R5730 |
T12200 |
T12195 |
punct |
.,immunoreactivity |
R5731 |
T12202 |
T12203 |
punct |
(,A |
R5732 |
T12203 |
T12204 |
meta |
A,consists |
R5733 |
T12205 |
T12203 |
punct |
),A |
R5734 |
T12206 |
T12207 |
compound |
Phase,contrast |
R5735 |
T12207 |
T12204 |
dep |
contrast,consists |
R5736 |
T12208 |
T12204 |
punct |
", ",consists |
R5737 |
T12209 |
T12210 |
compound |
embryo,E5 |
R5738 |
T12210 |
T12204 |
dep |
E5,consists |
R5739 |
T12211 |
T12204 |
punct |
: ,consists |
R5740 |
T12212 |
T12213 |
det |
The,cylinder |
R5741 |
T12213 |
T12204 |
nsubj |
cylinder,consists |
R5742 |
T12214 |
T12213 |
compound |
egg,cylinder |
R5743 |
T12215 |
T12204 |
prep |
of,consists |
R5744 |
T12216 |
T12217 |
det |
an,mass |
R5745 |
T12217 |
T12215 |
pobj |
mass,of |
R5746 |
T12218 |
T12217 |
amod |
inner,mass |
R5747 |
T12219 |
T12217 |
compound |
cell,mass |
R5748 |
T12220 |
T12221 |
punct |
(,a |
R5749 |
T12221 |
T12217 |
parataxis |
a,mass |
R5750 |
T12222 |
T12221 |
punct |
),a |
R5751 |
T12223 |
T12217 |
acl |
representing,mass |
R5752 |
T12224 |
T12225 |
det |
the,ectoderm |
R5753 |
T12225 |
T12223 |
dobj |
ectoderm,representing |
R5754 |
T12226 |
T12217 |
cc |
and,mass |
R5755 |
T12227 |
T12228 |
det |
an,layer |
R5756 |
T12228 |
T12217 |
conj |
layer,mass |
R5757 |
T12229 |
T12228 |
amod |
outer,layer |
R5758 |
T12230 |
T12228 |
prep |
of,layer |
R5759 |
T12231 |
T12232 |
compound |
endoderm,cells |
R5760 |
T12232 |
T12230 |
pobj |
cells,of |
R5761 |
T12233 |
T12234 |
punct |
(,b |
R5762 |
T12234 |
T12228 |
parataxis |
b,layer |
R5763 |
T12235 |
T12234 |
punct |
),b |
R5764 |
T12236 |
T12204 |
punct |
.,consists |
R5765 |
T12238 |
T12239 |
punct |
(,B |
R5766 |
T12239 |
T12240 |
meta |
B,performed |
R5767 |
T12241 |
T12239 |
punct |
),B |
R5768 |
T12242 |
T12240 |
nsubjpass |
Immunostaining,performed |
R5769 |
T12243 |
T12242 |
prep |
of,Immunostaining |
R5770 |
T12244 |
T12245 |
det |
the,section |
R5771 |
T12245 |
T12243 |
pobj |
section,of |
R5772 |
T12246 |
T12245 |
compound |
paraffin,section |
R5773 |
T12247 |
T12240 |
auxpass |
was,performed |
R5774 |
T12248 |
T12249 |
aux |
using,purified |
R5775 |
T12249 |
T12240 |
advcl |
purified,performed |
R5776 |
T12250 |
T12251 |
nmod |
mAb,203 |
R5777 |
T12251 |
T12249 |
dobj |
203,purified |
R5778 |
T12252 |
T12253 |
punct |
–,217 |
R5779 |
T12253 |
T12251 |
prep |
217,203 |
R5780 |
T12254 |
T12251 |
cc |
and,203 |
R5781 |
T12255 |
T12256 |
npadvmod |
Cy3,conjugated |
R5782 |
T12256 |
T12258 |
amod |
conjugated,IgG |
R5783 |
T12257 |
T12256 |
punct |
-,conjugated |
R5784 |
T12258 |
T12251 |
conj |
IgG,203 |
R5785 |
T12259 |
T12258 |
amod |
anti-mouse,IgG |
R5786 |
T12260 |
T12240 |
punct |
.,performed |
R5787 |
T12262 |
T12263 |
compound |
Annexin,A7 |
R5788 |
T12263 |
T12264 |
nsubjpass |
A7,expressed |
R5789 |
T12265 |
T12264 |
auxpass |
is,expressed |
R5790 |
T12266 |
T12264 |
prep |
in,expressed |
R5791 |
T12267 |
T12268 |
det |
both,types |
R5792 |
T12268 |
T12266 |
pobj |
types,in |
R5793 |
T12269 |
T12268 |
compound |
cell,types |
R5794 |
T12270 |
T12268 |
prep |
of,types |
R5795 |
T12271 |
T12272 |
det |
the,cylinder |
R5796 |
T12272 |
T12270 |
pobj |
cylinder,of |
R5797 |
T12273 |
T12272 |
compound |
egg,cylinder |
R5798 |
T12274 |
T12264 |
prep |
with,expressed |
R5799 |
T12275 |
T12276 |
det |
a,staining |
R5800 |
T12276 |
T12274 |
pobj |
staining,with |
R5801 |
T12277 |
T12276 |
amod |
strong,staining |
R5802 |
T12278 |
T12276 |
prep |
of,staining |
R5803 |
T12279 |
T12280 |
det |
the,endoderm |
R5804 |
T12280 |
T12278 |
pobj |
endoderm,of |
R5805 |
T12281 |
T12276 |
cc |
and,staining |
R5806 |
T12282 |
T12283 |
det |
a,staining |
R5807 |
T12283 |
T12276 |
conj |
staining,staining |
R5808 |
T12284 |
T12283 |
amod |
weaker,staining |
R5809 |
T12285 |
T12283 |
prep |
of,staining |
R5810 |
T12286 |
T12287 |
det |
the,ectoderm |
R5811 |
T12287 |
T12285 |
pobj |
ectoderm,of |
R5812 |
T12288 |
T12264 |
punct |
.,expressed |
R5813 |
T12290 |
T12291 |
det |
The,nuclei |
R5814 |
T12291 |
T12292 |
nsubj |
nuclei,are |
R5815 |
T12293 |
T12292 |
acomp |
devoid,are |
R5816 |
T12294 |
T12293 |
prep |
of,devoid |
R5817 |
T12295 |
T12296 |
amod |
immune,reactions |
R5818 |
T12296 |
T12294 |
pobj |
reactions,of |
R5819 |
T12297 |
T12292 |
punct |
.,are |
R5820 |
T12299 |
T12300 |
punct |
(,control |
R5821 |
T12301 |
T12300 |
meta |
C,control |
R5822 |
T12302 |
T12300 |
punct |
),control |
R5823 |
T12303 |
T12300 |
amod |
Negative,control |
R5824 |
T12304 |
T12300 |
acl |
using,control |
R5825 |
T12305 |
T12306 |
det |
the,antibody |
R5826 |
T12306 |
T12304 |
dobj |
antibody,using |
R5827 |
T12307 |
T12306 |
amod |
secondary,antibody |
R5828 |
T12308 |
T12306 |
compound |
Cy3,antibody |
R5829 |
T12309 |
T12306 |
punct |
-,antibody |
R5830 |
T12310 |
T12306 |
advmod |
only,antibody |
R5831 |
T12311 |
T12300 |
punct |
.,control |
R5832 |
T12313 |
T12314 |
punct |
(,D |
R5833 |
T12314 |
T12315 |
meta |
D,section |
R5834 |
T12316 |
T12314 |
punct |
-,D |
R5835 |
T12317 |
T12314 |
dep |
F,D |
R5836 |
T12318 |
T12314 |
punct |
),D |
R5837 |
T12319 |
T12320 |
compound |
Annexin,A7 |
R5838 |
T12320 |
T12321 |
compound |
A7,expression |
R5839 |
T12321 |
T12315 |
dep |
expression,section |
R5840 |
T12322 |
T12321 |
prep |
in,expression |
R5841 |
T12323 |
T12324 |
det |
the,tube |
R5842 |
T12324 |
T12322 |
pobj |
tube,in |
R5843 |
T12325 |
T12324 |
amod |
proximal,tube |
R5844 |
T12326 |
T12324 |
amod |
neural,tube |
R5845 |
T12327 |
T12328 |
punct |
(,D |
R5846 |
T12328 |
T12324 |
parataxis |
D,tube |
R5847 |
T12329 |
T12328 |
punct |
),D |
R5848 |
T12330 |
T12324 |
cc |
and,tube |
R5849 |
T12331 |
T12332 |
amod |
nearby,fold |
R5850 |
T12332 |
T12324 |
conj |
fold,tube |
R5851 |
T12333 |
T12332 |
amod |
neural,fold |
R5852 |
T12334 |
T12335 |
punct |
(,F |
R5853 |
T12335 |
T12332 |
parataxis |
F,fold |
R5854 |
T12336 |
T12335 |
nmod |
E,F |
R5855 |
T12337 |
T12335 |
punct |
",",F |
R5856 |
T12338 |
T12335 |
punct |
),F |
R5857 |
T12339 |
T12315 |
punct |
", ",section |
R5858 |
T12340 |
T12341 |
compound |
embryo,E8 |
R5859 |
T12341 |
T12315 |
dep |
E8,section |
R5860 |
T12342 |
T12315 |
punct |
", ",section |
R5861 |
T12343 |
T12315 |
amod |
transverse,section |
R5862 |
T12344 |
T12315 |
punct |
.,section |
R5863 |
T12346 |
T12347 |
nsubjpass |
Immunolabeling,performed |
R5864 |
T12348 |
T12346 |
prep |
of,Immunolabeling |
R5865 |
T12349 |
T12350 |
compound |
Annexin,A7 |
R5866 |
T12350 |
T12348 |
pobj |
A7,of |
R5867 |
T12351 |
T12347 |
auxpass |
was,performed |
R5868 |
T12352 |
T12347 |
prep |
with,performed |
R5869 |
T12353 |
T12354 |
amod |
purified,203 |
R5870 |
T12354 |
T12352 |
pobj |
203,with |
R5871 |
T12355 |
T12354 |
nmod |
mAb,203 |
R5872 |
T12356 |
T12357 |
punct |
–,217 |
R5873 |
T12357 |
T12354 |
prep |
217,203 |
R5874 |
T12358 |
T12347 |
cc |
and,performed |
R5875 |
T12359 |
T12360 |
nsubj |
visualization,was |
R5876 |
T12360 |
T12347 |
conj |
was,performed |
R5877 |
T12361 |
T12360 |
prep |
with,was |
R5878 |
T12362 |
T12363 |
det |
an,IgG |
R5879 |
T12363 |
T12361 |
pobj |
IgG,with |
R5880 |
T12364 |
T12365 |
compound |
Alexa,Fluor |
R5881 |
T12365 |
T12366 |
npadvmod |
Fluor,conjugated |
R5882 |
T12366 |
T12363 |
amod |
conjugated,IgG |
R5883 |
T12367 |
T12365 |
nummod |
488,Fluor |
R5884 |
T12368 |
T12366 |
punct |
-,conjugated |
R5885 |
T12369 |
T12363 |
amod |
anti-mouse,IgG |
R5886 |
T12370 |
T12360 |
punct |
.,was |
R5887 |
T12372 |
T12373 |
punct |
(,D |
R5888 |
T12373 |
T12374 |
meta |
D,is |
R5889 |
T12375 |
T12373 |
punct |
),D |
R5890 |
T12376 |
T12377 |
det |
An,immunostaining |
R5891 |
T12377 |
T12374 |
nsubj |
immunostaining,is |
R5892 |
T12378 |
T12377 |
amod |
intense,immunostaining |
R5893 |
T12379 |
T12380 |
compound |
Annexin,A7 |
R5894 |
T12380 |
T12377 |
compound |
A7,immunostaining |
R5895 |
T12381 |
T12374 |
acomp |
detectable,is |
R5896 |
T12382 |
T12374 |
prep |
in,is |
R5897 |
T12383 |
T12384 |
det |
the,neuroepithelium |
R5898 |
T12384 |
T12382 |
pobj |
neuroepithelium,in |
R5899 |
T12385 |
T12384 |
prep |
of,neuroepithelium |
R5900 |
T12386 |
T12387 |
det |
the,tube |
R5901 |
T12387 |
T12385 |
pobj |
tube,of |
R5902 |
T12388 |
T12387 |
amod |
neural,tube |
R5903 |
T12389 |
T12390 |
punct |
(,lumen |
R5904 |
T12390 |
T12374 |
parataxis |
lumen,is |
R5905 |
T12391 |
T12390 |
dep |
a,lumen |
R5906 |
T12392 |
T12390 |
punct |
", ",lumen |
R5907 |
T12393 |
T12390 |
prep |
of,lumen |
R5908 |
T12394 |
T12395 |
amod |
neural,tube |
R5909 |
T12395 |
T12393 |
pobj |
tube,of |
R5910 |
T12396 |
T12390 |
punct |
),lumen |
R5911 |
T12397 |
T12374 |
punct |
.,is |
R5912 |
T12399 |
T12400 |
punct |
(,E |
R5913 |
T12400 |
T12401 |
meta |
E,show |
R5914 |
T12402 |
T12400 |
punct |
",",E |
R5915 |
T12403 |
T12400 |
dep |
F,E |
R5916 |
T12404 |
T12400 |
punct |
),E |
R5917 |
T12405 |
T12406 |
amod |
Higher,magnifications |
R5918 |
T12406 |
T12401 |
nsubj |
magnifications,show |
R5919 |
T12407 |
T12406 |
prep |
of,magnifications |
R5920 |
T12408 |
T12409 |
det |
the,neuroepithelium |
R5921 |
T12409 |
T12407 |
pobj |
neuroepithelium,of |
R5922 |
T12410 |
T12411 |
mark |
that,expressed |
R5923 |
T12411 |
T12401 |
ccomp |
expressed,show |
R5924 |
T12412 |
T12413 |
compound |
Annexin,A7 |
R5925 |
T12413 |
T12411 |
nsubjpass |
A7,expressed |
R5926 |
T12414 |
T12411 |
auxpass |
is,expressed |
R5927 |
T12415 |
T12411 |
prep |
in,expressed |
R5928 |
T12416 |
T12417 |
det |
the,cytosol |
R5929 |
T12417 |
T12415 |
pobj |
cytosol,in |
R5930 |
T12418 |
T12401 |
punct |
.,show |
R5931 |
T12420 |
T12421 |
nsubj |
Arrowheads,point |
R5932 |
T12422 |
T12421 |
prep |
to,point |
R5933 |
T12423 |
T12424 |
compound |
Annexin,A7 |
R5934 |
T12424 |
T12425 |
compound |
A7,immunoreactivity |
R5935 |
T12425 |
T12422 |
pobj |
immunoreactivity,to |
R5936 |
T12426 |
T12421 |
prep |
in,point |
R5937 |
T12427 |
T12428 |
det |
the,cytosol |
R5938 |
T12428 |
T12426 |
pobj |
cytosol,in |
R5939 |
T12429 |
T12421 |
punct |
.,point |
R5940 |
T12431 |
T12432 |
punct |
(,tube |
R5941 |
T12433 |
T12432 |
meta |
G,tube |
R5942 |
T12434 |
T12432 |
punct |
),tube |
R5943 |
T12435 |
T12436 |
compound |
Phase,contrast |
R5944 |
T12436 |
T12432 |
dep |
contrast,tube |
R5945 |
T12437 |
T12432 |
punct |
", ",tube |
R5946 |
T12438 |
T12439 |
compound |
embryo,E13 |
R5947 |
T12439 |
T12432 |
dep |
E13,tube |
R5948 |
T12440 |
T12432 |
punct |
", ",tube |
R5949 |
T12441 |
T12432 |
amod |
caudal,tube |
R5950 |
T12442 |
T12432 |
amod |
neural,tube |
R5951 |
T12443 |
T12432 |
punct |
.,tube |
R5952 |
T12445 |
T12446 |
punct |
(,H |
R5953 |
T12446 |
T12447 |
meta |
H,performed |
R5954 |
T12448 |
T12446 |
punct |
),H |
R5955 |
T12449 |
T12447 |
nsubjpass |
Immunostaining,performed |
R5956 |
T12450 |
T12449 |
prep |
of,Immunostaining |
R5957 |
T12451 |
T12452 |
det |
the,section |
R5958 |
T12452 |
T12450 |
pobj |
section,of |
R5959 |
T12453 |
T12452 |
compound |
paraffin,section |
R5960 |
T12454 |
T12447 |
auxpass |
was,performed |
R5961 |
T12455 |
T12447 |
advcl |
using,performed |
R5962 |
T12456 |
T12457 |
amod |
purified,203 |
R5963 |
T12457 |
T12455 |
dobj |
203,using |
R5964 |
T12458 |
T12457 |
nmod |
mAb,203 |
R5965 |
T12459 |
T12460 |
punct |
–,217 |
R5966 |
T12460 |
T12457 |
prep |
217,203 |
R5967 |
T12461 |
T12457 |
cc |
and,203 |
R5968 |
T12462 |
T12463 |
compound |
Alexa,Fluor |
R5969 |
T12463 |
T12464 |
npadvmod |
Fluor,conjugated |
R5970 |
T12464 |
T12467 |
amod |
conjugated,IgG |
R5971 |
T12465 |
T12463 |
nummod |
488,Fluor |
R5972 |
T12466 |
T12464 |
punct |
-,conjugated |
R5973 |
T12467 |
T12457 |
conj |
IgG,203 |
R5974 |
T12468 |
T12467 |
amod |
anti-mouse,IgG |
R5975 |
T12469 |
T12447 |
punct |
.,performed |
R5976 |
T12471 |
T12472 |
punct |
(,I |
R5977 |
T12472 |
T12473 |
meta |
I,control |
R5978 |
T12474 |
T12472 |
punct |
),I |
R5979 |
T12475 |
T12473 |
amod |
Negative,control |
R5980 |
T12476 |
T12473 |
acl |
using,control |
R5981 |
T12477 |
T12478 |
det |
the,antibody |
R5982 |
T12478 |
T12476 |
dobj |
antibody,using |
R5983 |
T12479 |
T12478 |
amod |
secondary,antibody |
R5984 |
T12480 |
T12481 |
nmod |
Alexa,Fluor |
R5985 |
T12481 |
T12478 |
nmod |
Fluor,antibody |
R5986 |
T12482 |
T12481 |
nummod |
488,Fluor |
R5987 |
T12483 |
T12478 |
punct |
-,antibody |
R5988 |
T12484 |
T12478 |
advmod |
only,antibody |
R5989 |
T12485 |
T12473 |
punct |
.,control |
R5990 |
T12488 |
T12487 |
punct |
", ",Bar |
R5991 |
T12489 |
T12490 |
nummod |
20,μm |
R5992 |
T12490 |
T12487 |
appos |
μm,Bar |
R5993 |
T12491 |
T12487 |
punct |
.,Bar |
R5996 |
T12790 |
T12791 |
amod |
Subcellular,localization |
R5997 |
T12792 |
T12791 |
prep |
of,localization |
R5998 |
T12793 |
T12794 |
compound |
Annexin,A7 |
R5999 |
T12794 |
T12792 |
pobj |
A7,of |
R6000 |
T12795 |
T12791 |
prep |
in,localization |
R6001 |
T12796 |
T12797 |
compound |
embryos,E13 |
R6002 |
T12797 |
T12795 |
pobj |
E13,in |
R6003 |
T12798 |
T12797 |
punct |
", ",E13 |
R6004 |
T12799 |
T12797 |
conj |
E15,E13 |
R6005 |
T12800 |
T12799 |
cc |
and,E15 |
R6006 |
T12801 |
T12799 |
conj |
E16,E15 |
R6007 |
T12802 |
T12791 |
punct |
.,localization |
R6008 |
T12804 |
T12805 |
compound |
Paraffin,sections |
R6009 |
T12805 |
T12806 |
nsubjpass |
sections,stained |
R6010 |
T12807 |
T12805 |
prep |
of,sections |
R6011 |
T12808 |
T12809 |
amod |
embryonic,brain |
R6012 |
T12809 |
T12807 |
pobj |
brain,of |
R6013 |
T12810 |
T12806 |
auxpass |
were,stained |
R6014 |
T12811 |
T12806 |
prep |
with,stained |
R6015 |
T12812 |
T12813 |
amod |
purified,203 |
R6016 |
T12813 |
T12811 |
pobj |
203,with |
R6017 |
T12814 |
T12813 |
nmod |
mAb,203 |
R6018 |
T12815 |
T12816 |
punct |
–,217 |
R6019 |
T12816 |
T12813 |
prep |
217,203 |
R6020 |
T12817 |
T12806 |
punct |
.,stained |
R6021 |
T12819 |
T12820 |
compound |
Annexin,A7 |
R6022 |
T12820 |
T12821 |
nsubjpass |
A7,visualized |
R6023 |
T12822 |
T12821 |
auxpass |
was,visualized |
R6024 |
T12823 |
T12821 |
prep |
with,visualized |
R6025 |
T12824 |
T12825 |
compound |
Alexa,Fluor |
R6026 |
T12825 |
T12826 |
npadvmod |
Fluor,conjugated |
R6027 |
T12826 |
T12829 |
amod |
conjugated,antibody |
R6028 |
T12827 |
T12825 |
nummod |
488,Fluor |
R6029 |
T12828 |
T12826 |
punct |
-,conjugated |
R6030 |
T12829 |
T12823 |
pobj |
antibody,with |
R6031 |
T12830 |
T12829 |
amod |
secondary,antibody |
R6032 |
T12831 |
T12821 |
punct |
.,visualized |
R6033 |
T12833 |
T12834 |
punct |
(,A |
R6034 |
T12834 |
T12835 |
meta |
A,given |
R6035 |
T12836 |
T12834 |
punct |
),A |
R6036 |
T12837 |
T12838 |
dep |
Overview,stained |
R6037 |
T12838 |
T12835 |
dep |
stained,given |
R6038 |
T12839 |
T12838 |
punct |
", ",stained |
R6039 |
T12840 |
T12838 |
prep |
at,stained |
R6040 |
T12841 |
T12840 |
pobj |
E16,at |
R6041 |
T12842 |
T12843 |
det |
the,cells |
R6042 |
T12843 |
T12838 |
nsubjpass |
cells,stained |
R6043 |
T12844 |
T12843 |
amod |
immature,cells |
R6044 |
T12845 |
T12846 |
npadvmod |
GFAP,negative |
R6045 |
T12846 |
T12843 |
amod |
negative,cells |
R6046 |
T12847 |
T12846 |
punct |
-,negative |
R6047 |
T12848 |
T12843 |
prep |
of,cells |
R6048 |
T12849 |
T12850 |
det |
the,neocortex |
R6049 |
T12850 |
T12848 |
pobj |
neocortex,of |
R6050 |
T12851 |
T12850 |
amod |
forming,neocortex |
R6051 |
T12852 |
T12850 |
amod |
cerebral,neocortex |
R6052 |
T12853 |
T12854 |
punct |
(,b |
R6053 |
T12854 |
T12843 |
parataxis |
b,cells |
R6054 |
T12855 |
T12854 |
punct |
),b |
R6055 |
T12856 |
T12843 |
acl |
surrounding,cells |
R6056 |
T12857 |
T12858 |
det |
the,ventricle |
R6057 |
T12858 |
T12856 |
dobj |
ventricle,surrounding |
R6058 |
T12859 |
T12858 |
amod |
lateral,ventricle |
R6059 |
T12860 |
T12861 |
punct |
(,a |
R6060 |
T12861 |
T12858 |
parataxis |
a,ventricle |
R6061 |
T12862 |
T12861 |
punct |
),a |
R6062 |
T12863 |
T12838 |
auxpass |
are,stained |
R6063 |
T12864 |
T12838 |
advmod |
strongly,stained |
R6064 |
T12865 |
T12835 |
punct |
;,given |
R6065 |
T12866 |
T12835 |
dep |
square,given |
R6066 |
T12867 |
T12835 |
punct |
", ",given |
R6067 |
T12868 |
T12869 |
det |
a,magnification |
R6068 |
T12869 |
T12835 |
nsubjpass |
magnification,given |
R6069 |
T12870 |
T12869 |
amod |
higher,magnification |
R6070 |
T12871 |
T12869 |
prep |
of,magnification |
R6071 |
T12872 |
T12873 |
det |
this,area |
R6072 |
T12873 |
T12871 |
pobj |
area,of |
R6073 |
T12874 |
T12835 |
auxpass |
is,given |
R6074 |
T12875 |
T12835 |
prep |
in,given |
R6075 |
T12876 |
T12875 |
punct |
(,in |
R6076 |
T12877 |
T12875 |
pobj |
I,in |
R6077 |
T12878 |
T12835 |
punct |
),given |
R6078 |
T12879 |
T12835 |
punct |
.,given |
R6079 |
T12881 |
T12882 |
punct |
(,B |
R6080 |
T12882 |
T12883 |
meta |
B,shows |
R6081 |
T12884 |
T12882 |
punct |
),B |
R6082 |
T12885 |
T12886 |
amod |
Higher,magnification |
R6083 |
T12886 |
T12883 |
nsubj |
magnification,shows |
R6084 |
T12887 |
T12886 |
prep |
of,magnification |
R6085 |
T12888 |
T12889 |
det |
the,E13 |
R6086 |
T12889 |
T12887 |
pobj |
E13,of |
R6087 |
T12890 |
T12889 |
amod |
earlier,E13 |
R6088 |
T12891 |
T12889 |
compound |
stage,E13 |
R6089 |
T12892 |
T12893 |
punct |
(,H |
R6090 |
T12893 |
T12886 |
parataxis |
H,magnification |
R6091 |
T12894 |
T12893 |
punct |
),H |
R6092 |
T12895 |
T12883 |
punct |
", ",shows |
R6093 |
T12896 |
T12897 |
mark |
that,stained |
R6094 |
T12897 |
T12883 |
ccomp |
stained,shows |
R6095 |
T12898 |
T12899 |
det |
the,cells |
R6096 |
T12899 |
T12897 |
nsubjpass |
cells,stained |
R6097 |
T12900 |
T12897 |
auxpass |
are,stained |
R6098 |
T12901 |
T12897 |
prep |
in,stained |
R6099 |
T12902 |
T12903 |
det |
the,cytosol |
R6100 |
T12903 |
T12901 |
pobj |
cytosol,in |
R6101 |
T12904 |
T12905 |
punct |
(,arrowhead |
R6102 |
T12905 |
T12897 |
parataxis |
arrowhead,stained |
R6103 |
T12906 |
T12905 |
punct |
),arrowhead |
R6104 |
T12907 |
T12883 |
punct |
.,shows |
R6105 |
T12909 |
T12910 |
punct |
(,C |
R6106 |
T12910 |
T12911 |
meta |
C,rounded |
R6107 |
T12912 |
T12910 |
punct |
),C |
R6108 |
T12913 |
T12914 |
nummod |
Two,days |
R6109 |
T12914 |
T12915 |
npadvmod |
days,later |
R6110 |
T12915 |
T12911 |
advmod |
later,rounded |
R6111 |
T12916 |
T12911 |
prep |
at,rounded |
R6112 |
T12917 |
T12916 |
pobj |
E15,at |
R6113 |
T12918 |
T12919 |
det |
the,cells |
R6114 |
T12919 |
T12911 |
nsubj |
cells,rounded |
R6115 |
T12920 |
T12911 |
aux |
have,rounded |
R6116 |
T12921 |
T12911 |
prt |
up,rounded |
R6117 |
T12922 |
T12911 |
punct |
", ",rounded |
R6118 |
T12923 |
T12911 |
cc |
and,rounded |
R6119 |
T12924 |
T12925 |
compound |
Annexin,A7 |
R6120 |
T12925 |
T12926 |
nsubj |
A7,stays |
R6121 |
T12926 |
T12911 |
conj |
stays,rounded |
R6122 |
T12927 |
T12926 |
prep |
in,stays |
R6123 |
T12928 |
T12929 |
det |
the,cytosol |
R6124 |
T12929 |
T12927 |
pobj |
cytosol,in |
R6125 |
T12930 |
T12911 |
punct |
.,rounded |
R6126 |
T12932 |
T12933 |
punct |
(,D |
R6127 |
T12933 |
T12934 |
meta |
D,becomes |
R6128 |
T12935 |
T12933 |
punct |
),D |
R6129 |
T12936 |
T12934 |
prep |
At,becomes |
R6130 |
T12937 |
T12936 |
pobj |
E16,At |
R6131 |
T12938 |
T12939 |
det |
the,staining |
R6132 |
T12939 |
T12934 |
nsubj |
staining,becomes |
R6133 |
T12940 |
T12939 |
amod |
first,staining |
R6134 |
T12941 |
T12939 |
amod |
nuclear,staining |
R6135 |
T12942 |
T12934 |
acomp |
apparent,becomes |
R6136 |
T12943 |
T12944 |
punct |
(,arrowhead |
R6137 |
T12944 |
T12934 |
parataxis |
arrowhead,becomes |
R6138 |
T12945 |
T12944 |
punct |
),arrowhead |
R6139 |
T12946 |
T12934 |
prep |
in,becomes |
R6140 |
T12947 |
T12946 |
pobj |
cells,in |
R6141 |
T12948 |
T12947 |
prep |
of,cells |
R6142 |
T12949 |
T12950 |
det |
the,zone |
R6143 |
T12950 |
T12948 |
pobj |
zone,of |
R6144 |
T12951 |
T12950 |
amod |
intermediate,zone |
R6145 |
T12952 |
T12950 |
acl |
located,zone |
R6146 |
T12953 |
T12952 |
prep |
between,located |
R6147 |
T12954 |
T12955 |
amod |
ventricular,zone |
R6148 |
T12955 |
T12953 |
pobj |
zone,between |
R6149 |
T12956 |
T12955 |
amod |
germinative,zone |
R6150 |
T12957 |
T12955 |
cc |
and,zone |
R6151 |
T12958 |
T12959 |
amod |
marginal,cortex |
R6152 |
T12959 |
T12955 |
conj |
cortex,zone |
R6153 |
T12960 |
T12959 |
amod |
neopallial,cortex |
R6154 |
T12961 |
T12962 |
mark |
as,seen |
R6155 |
T12962 |
T12934 |
advcl |
seen,becomes |
R6156 |
T12963 |
T12962 |
prep |
in,seen |
R6157 |
T12964 |
T12963 |
punct |
(,in |
R6158 |
T12965 |
T12963 |
pobj |
I,in |
R6159 |
T12966 |
T12963 |
punct |
),in |
R6160 |
T12967 |
T12968 |
punct |
", ",oval |
R6161 |
T12968 |
T12963 |
parataxis |
oval,in |
R6162 |
T12969 |
T12934 |
punct |
.,becomes |
R6163 |
T12971 |
T12972 |
punct |
(,E |
R6164 |
T12972 |
T12973 |
meta |
E,confirms |
R6165 |
T12974 |
T12972 |
punct |
-,E |
R6166 |
T12975 |
T12972 |
dep |
G,E |
R6167 |
T12976 |
T12972 |
punct |
),E |
R6168 |
T12977 |
T12978 |
amod |
Confocal,microscopy |
R6169 |
T12978 |
T12973 |
nsubj |
microscopy,confirms |
R6170 |
T12979 |
T12980 |
det |
the,results |
R6171 |
T12980 |
T12973 |
dobj |
results,confirms |
R6172 |
T12981 |
T12980 |
prep |
in,results |
R6173 |
T12982 |
T12981 |
pobj |
B,in |
R6174 |
T12983 |
T12984 |
punct |
-,D |
R6175 |
T12984 |
T12982 |
prep |
D,B |
R6176 |
T12985 |
T12973 |
punct |
.,confirms |
R6177 |
T12987 |
T12988 |
punct |
(,H |
R6178 |
T12988 |
T12989 |
meta |
H,stained |
R6179 |
T12990 |
T12988 |
punct |
),H |
R6180 |
T12991 |
T12989 |
dep |
Overview,stained |
R6181 |
T12992 |
T12989 |
punct |
", ",stained |
R6182 |
T12993 |
T12989 |
prep |
at,stained |
R6183 |
T12994 |
T12993 |
pobj |
E13,at |
R6184 |
T12995 |
T12996 |
det |
the,cells |
R6185 |
T12996 |
T12989 |
nsubjpass |
cells,stained |
R6186 |
T12997 |
T12996 |
amod |
immature,cells |
R6187 |
T12998 |
T12999 |
npadvmod |
GFAP,negative |
R6188 |
T12999 |
T12996 |
amod |
negative,cells |
R6189 |
T13000 |
T12999 |
punct |
-,negative |
R6190 |
T13001 |
T12989 |
auxpass |
are,stained |
R6191 |
T13002 |
T12989 |
advmod |
strongly,stained |
R6192 |
T13003 |
T13004 |
punct |
;,ventricle |
R6193 |
T13004 |
T12989 |
parataxis |
ventricle,stained |
R6194 |
T13005 |
T13004 |
dep |
a,ventricle |
R6195 |
T13006 |
T13004 |
punct |
", ",ventricle |
R6196 |
T13007 |
T13004 |
amod |
lateral,ventricle |
R6197 |
T13008 |
T12989 |
punct |
.,stained |
R6198 |
T13010 |
T13011 |
punct |
(,I |
R6199 |
T13011 |
T13012 |
meta |
I,magnification |
R6200 |
T13013 |
T13011 |
punct |
),I |
R6201 |
T13014 |
T13012 |
amod |
Higher,magnification |
R6202 |
T13015 |
T13012 |
prep |
of,magnification |
R6203 |
T13016 |
T13017 |
det |
a,section |
R6204 |
T13017 |
T13015 |
pobj |
section,of |
R6205 |
T13018 |
T13017 |
amod |
cortical,section |
R6206 |
T13019 |
T13017 |
prep |
of,section |
R6207 |
T13020 |
T13019 |
punct |
(,of |
R6208 |
T13021 |
T13019 |
pobj |
A,of |
R6209 |
T13022 |
T13023 |
punct |
", ",square |
R6210 |
T13023 |
T13021 |
parataxis |
square,A |
R6211 |
T13024 |
T13023 |
punct |
),square |
R6212 |
T13025 |
T13012 |
acl |
demonstrating,magnification |
R6213 |
T13026 |
T13027 |
amod |
ventricular,zones |
R6214 |
T13027 |
T13025 |
dobj |
zones,demonstrating |
R6215 |
T13028 |
T13026 |
punct |
", ",ventricular |
R6216 |
T13029 |
T13026 |
conj |
intermediate,ventricular |
R6217 |
T13030 |
T13029 |
punct |
", ",intermediate |
R6218 |
T13031 |
T13029 |
cc |
and,intermediate |
R6219 |
T13032 |
T13029 |
conj |
marginal,intermediate |
R6220 |
T13033 |
T13012 |
punct |
;,magnification |
R6221 |
T13034 |
T13035 |
dep |
oval,given |
R6222 |
T13035 |
T13012 |
dep |
given,magnification |
R6223 |
T13036 |
T13035 |
punct |
", ",given |
R6224 |
T13037 |
T13038 |
det |
a,magnification |
R6225 |
T13038 |
T13035 |
nsubjpass |
magnification,given |
R6226 |
T13039 |
T13038 |
amod |
higher,magnification |
R6227 |
T13040 |
T13038 |
prep |
of,magnification |
R6228 |
T13041 |
T13042 |
det |
this,area |
R6229 |
T13042 |
T13040 |
pobj |
area,of |
R6230 |
T13043 |
T13035 |
auxpass |
is,given |
R6231 |
T13044 |
T13035 |
prep |
in,given |
R6232 |
T13045 |
T13044 |
punct |
(,in |
R6233 |
T13046 |
T13047 |
nmod |
D,G |
R6234 |
T13047 |
T13044 |
pobj |
G,in |
R6235 |
T13048 |
T13047 |
punct |
",",G |
R6236 |
T13049 |
T13012 |
punct |
),magnification |
R6237 |
T13050 |
T13012 |
punct |
.,magnification |
R6244 |
T13536 |
T13537 |
compound |
Annexin,A7 |
R6245 |
T13537 |
T13538 |
nsubj |
A7,is |
R6246 |
T13539 |
T13538 |
acomp |
present,is |
R6247 |
T13540 |
T13538 |
prep |
in,is |
R6248 |
T13541 |
T13540 |
pobj |
neurons,in |
R6249 |
T13542 |
T13541 |
cc |
and,neurons |
R6250 |
T13543 |
T13541 |
conj |
astrocytes,neurons |
R6251 |
T13544 |
T13541 |
prep |
of,neurons |
R6252 |
T13545 |
T13546 |
det |
the,temporalis |
R6253 |
T13546 |
T13544 |
pobj |
temporalis,of |
R6254 |
T13547 |
T13546 |
compound |
cortex,temporalis |
R6255 |
T13548 |
T13546 |
cc |
and,temporalis |
R6256 |
T13549 |
T13550 |
amod |
hippocampal,formation |
R6257 |
T13550 |
T13546 |
conj |
formation,temporalis |
R6258 |
T13551 |
T13546 |
prep |
of,temporalis |
R6259 |
T13552 |
T13553 |
nummod |
10,weeks |
R6260 |
T13553 |
T13555 |
npadvmod |
weeks,old |
R6261 |
T13554 |
T13553 |
punct |
-,weeks |
R6262 |
T13555 |
T13557 |
amod |
old,mice |
R6263 |
T13556 |
T13555 |
punct |
-,old |
R6264 |
T13557 |
T13551 |
pobj |
mice,of |
R6265 |
T13558 |
T13538 |
punct |
.,is |
R6266 |
T13560 |
T13561 |
punct |
(,A |
R6267 |
T13561 |
T13562 |
meta |
A,presents |
R6268 |
T13563 |
T13561 |
punct |
),A |
R6269 |
T13564 |
T13565 |
amod |
Low,magnification |
R6270 |
T13565 |
T13562 |
nsubj |
magnification,presents |
R6271 |
T13566 |
T13565 |
prep |
of,magnification |
R6272 |
T13567 |
T13568 |
det |
the,temporalis |
R6273 |
T13568 |
T13566 |
pobj |
temporalis,of |
R6274 |
T13569 |
T13568 |
compound |
cortex,temporalis |
R6275 |
T13570 |
T13571 |
det |
an,expression |
R6276 |
T13571 |
T13562 |
dobj |
expression,presents |
R6277 |
T13572 |
T13573 |
compound |
Annexin,A7 |
R6278 |
T13573 |
T13571 |
compound |
A7,expression |
R6279 |
T13574 |
T13571 |
prep |
in,expression |
R6280 |
T13575 |
T13574 |
pobj |
cells,in |
R6281 |
T13576 |
T13575 |
prep |
of,cells |
R6282 |
T13577 |
T13578 |
det |
the,border |
R6283 |
T13578 |
T13576 |
pobj |
border,of |
R6284 |
T13579 |
T13578 |
amod |
pial,border |
R6285 |
T13580 |
T13571 |
punct |
", ",expression |
R6286 |
T13581 |
T13571 |
prep |
in,expression |
R6287 |
T13582 |
T13581 |
pobj |
neurons,in |
R6288 |
T13583 |
T13582 |
prep |
of,neurons |
R6289 |
T13584 |
T13585 |
det |
all,laminae |
R6290 |
T13585 |
T13583 |
pobj |
laminae,of |
R6291 |
T13586 |
T13585 |
nummod |
six,laminae |
R6292 |
T13587 |
T13585 |
amod |
isocortical,laminae |
R6293 |
T13588 |
T13571 |
punct |
", ",expression |
R6294 |
T13589 |
T13571 |
cc |
and,expression |
R6295 |
T13590 |
T13591 |
det |
a,signal |
R6296 |
T13591 |
T13571 |
conj |
signal,expression |
R6297 |
T13592 |
T13591 |
amod |
weak,signal |
R6298 |
T13593 |
T13591 |
prep |
in,signal |
R6299 |
T13594 |
T13595 |
det |
the,matter |
R6300 |
T13595 |
T13593 |
pobj |
matter,in |
R6301 |
T13596 |
T13595 |
amod |
adjacent,matter |
R6302 |
T13597 |
T13595 |
amod |
white,matter |
R6303 |
T13598 |
T13562 |
punct |
.,presents |
R6304 |
T13600 |
T13601 |
punct |
(,B |
R6305 |
T13601 |
T13602 |
meta |
B,section |
R6306 |
T13603 |
T13601 |
punct |
),B |
R6307 |
T13604 |
T13602 |
amod |
Corresponding,section |
R6308 |
T13605 |
T13602 |
acl |
stained,section |
R6309 |
T13606 |
T13605 |
prep |
with,stained |
R6310 |
T13607 |
T13606 |
pobj |
GFAP,with |
R6311 |
T13608 |
T13602 |
punct |
.,section |
R6312 |
T13610 |
T13611 |
punct |
(,C |
R6313 |
T13611 |
T13612 |
meta |
C,Staining |
R6314 |
T13613 |
T13611 |
punct |
),C |
R6315 |
T13614 |
T13612 |
prep |
in,Staining |
R6316 |
T13615 |
T13616 |
det |
the,pyramidalis |
R6317 |
T13616 |
T13614 |
pobj |
pyramidalis,in |
R6318 |
T13617 |
T13616 |
compound |
Stratum,pyramidalis |
R6319 |
T13618 |
T13619 |
punct |
(,a |
R6320 |
T13619 |
T13616 |
parataxis |
a,pyramidalis |
R6321 |
T13620 |
T13619 |
punct |
),a |
R6322 |
T13621 |
T13614 |
cc |
and,in |
R6323 |
T13622 |
T13614 |
conj |
in,in |
R6324 |
T13623 |
T13624 |
det |
the,gyrus |
R6325 |
T13624 |
T13622 |
pobj |
gyrus,in |
R6326 |
T13625 |
T13624 |
compound |
dentate,gyrus |
R6327 |
T13626 |
T13624 |
prep |
of,gyrus |
R6328 |
T13627 |
T13628 |
det |
the,hippocampus |
R6329 |
T13628 |
T13626 |
pobj |
hippocampus,of |
R6330 |
T13629 |
T13612 |
punct |
;,Staining |
R6331 |
T13630 |
T13631 |
dep |
square,given |
R6332 |
T13631 |
T13612 |
dep |
given,Staining |
R6333 |
T13632 |
T13631 |
punct |
", ",given |
R6334 |
T13633 |
T13634 |
det |
a,magnification |
R6335 |
T13634 |
T13631 |
nsubjpass |
magnification,given |
R6336 |
T13635 |
T13634 |
amod |
higher,magnification |
R6337 |
T13636 |
T13634 |
prep |
of,magnification |
R6338 |
T13637 |
T13638 |
amod |
acorresponding,area |
R6339 |
T13638 |
T13636 |
pobj |
area,of |
R6340 |
T13639 |
T13631 |
auxpass |
is,given |
R6341 |
T13640 |
T13631 |
prep |
in,given |
R6342 |
T13641 |
T13640 |
punct |
(,in |
R6343 |
T13642 |
T13643 |
nmod |
G,H |
R6344 |
T13643 |
T13640 |
pobj |
H,in |
R6345 |
T13644 |
T13643 |
punct |
",",H |
R6346 |
T13645 |
T13640 |
punct |
),in |
R6347 |
T13646 |
T13612 |
punct |
.,Staining |
R6348 |
T13648 |
T13649 |
det |
An,immunostaining |
R6349 |
T13649 |
T13653 |
nsubj |
immunostaining,is |
R6350 |
T13650 |
T13649 |
amod |
intense,immunostaining |
R6351 |
T13651 |
T13652 |
compound |
Annexin,A7 |
R6352 |
T13652 |
T13649 |
compound |
A7,immunostaining |
R6353 |
T13654 |
T13653 |
acomp |
detectable,is |
R6354 |
T13655 |
T13653 |
punct |
.,is |
R6355 |
T13657 |
T13658 |
punct |
(,D |
R6356 |
T13658 |
T13659 |
meta |
D,section |
R6357 |
T13660 |
T13658 |
punct |
),D |
R6358 |
T13661 |
T13659 |
amod |
Corresponding,section |
R6359 |
T13662 |
T13659 |
acl |
stained,section |
R6360 |
T13663 |
T13662 |
prep |
with,stained |
R6361 |
T13664 |
T13665 |
amod |
secondary,antibody |
R6362 |
T13665 |
T13663 |
pobj |
antibody,with |
R6363 |
T13666 |
T13665 |
advmod |
only,antibody |
R6364 |
T13667 |
T13659 |
punct |
.,section |
R6365 |
T13669 |
T13670 |
punct |
(,E |
R6366 |
T13670 |
T13671 |
meta |
E,Presence |
R6367 |
T13672 |
T13670 |
punct |
),E |
R6368 |
T13673 |
T13671 |
prep |
of,Presence |
R6369 |
T13674 |
T13675 |
compound |
Annexin,A7 |
R6370 |
T13675 |
T13673 |
pobj |
A7,of |
R6371 |
T13676 |
T13671 |
prep |
in,Presence |
R6372 |
T13677 |
T13678 |
amod |
pyramidal,neurons |
R6373 |
T13678 |
T13676 |
pobj |
neurons,in |
R6374 |
T13679 |
T13680 |
punct |
(,externa |
R6375 |
T13680 |
T13678 |
parataxis |
externa,neurons |
R6376 |
T13681 |
T13680 |
compound |
lamina,externa |
R6377 |
T13682 |
T13680 |
compound |
pyramidalis,externa |
R6378 |
T13683 |
T13680 |
punct |
),externa |
R6379 |
T13684 |
T13678 |
prep |
of,neurons |
R6380 |
T13685 |
T13686 |
det |
the,temporalis |
R6381 |
T13686 |
T13684 |
pobj |
temporalis,of |
R6382 |
T13687 |
T13686 |
compound |
isocortex,temporalis |
R6383 |
T13688 |
T13671 |
punct |
.,Presence |
R6384 |
T13690 |
T13691 |
det |
These,neurons |
R6385 |
T13691 |
T13692 |
nsubjpass |
neurons,identified |
R6386 |
T13693 |
T13692 |
auxpass |
were,identified |
R6387 |
T13694 |
T13692 |
prep |
based,identified |
R6388 |
T13695 |
T13694 |
prep |
on,based |
R6389 |
T13696 |
T13697 |
poss |
their,morphology |
R6390 |
T13697 |
T13695 |
pobj |
morphology,on |
R6391 |
T13698 |
T13697 |
punct |
", ",morphology |
R6392 |
T13699 |
T13697 |
conj |
distribution,morphology |
R6393 |
T13700 |
T13699 |
cc |
and,distribution |
R6394 |
T13701 |
T13699 |
conj |
lack,distribution |
R6395 |
T13702 |
T13701 |
prep |
of,lack |
R6396 |
T13703 |
T13704 |
compound |
GFAP,staining |
R6397 |
T13704 |
T13702 |
pobj |
staining,of |
R6398 |
T13705 |
T13692 |
punct |
.,identified |
R6399 |
T13707 |
T13708 |
nsubj |
AnnexinA7,exhibits |
R6400 |
T13709 |
T13710 |
det |
a,staining |
R6401 |
T13710 |
T13708 |
dobj |
staining,exhibits |
R6402 |
T13711 |
T13710 |
compound |
punctate,staining |
R6403 |
T13712 |
T13710 |
punct |
", ",staining |
R6404 |
T13713 |
T13714 |
dep |
which,is |
R6405 |
T13714 |
T13710 |
relcl |
is,staining |
R6406 |
T13715 |
T13714 |
acomp |
pronounced,is |
R6407 |
T13716 |
T13714 |
prep |
in,is |
R6408 |
T13717 |
T13718 |
det |
the,nucleus |
R6409 |
T13718 |
T13716 |
pobj |
nucleus,in |
R6410 |
T13719 |
T13720 |
punct |
(,arrowhead |
R6411 |
T13720 |
T13708 |
parataxis |
arrowhead,exhibits |
R6412 |
T13721 |
T13720 |
punct |
),arrowhead |
R6413 |
T13722 |
T13708 |
punct |
.,exhibits |
R6414 |
T13724 |
T13725 |
punct |
(,F |
R6415 |
T13725 |
T13726 |
meta |
F,shows |
R6416 |
T13727 |
T13725 |
punct |
),F |
R6417 |
T13728 |
T13729 |
amod |
Higher,magnification |
R6418 |
T13729 |
T13726 |
nsubj |
magnification,shows |
R6419 |
T13730 |
T13729 |
prep |
of,magnification |
R6420 |
T13731 |
T13730 |
pobj |
image,of |
R6421 |
T13732 |
T13733 |
punct |
(,A |
R6422 |
T13733 |
T13729 |
parataxis |
A,magnification |
R6423 |
T13734 |
T13733 |
punct |
),A |
R6424 |
T13735 |
T13726 |
advmod |
also,shows |
R6425 |
T13736 |
T13737 |
compound |
Annexin,A7 |
R6426 |
T13737 |
T13726 |
dobj |
A7,shows |
R6427 |
T13738 |
T13726 |
prep |
in,shows |
R6428 |
T13739 |
T13738 |
pobj |
nuclei,in |
R6429 |
T13740 |
T13739 |
prep |
of,nuclei |
R6430 |
T13741 |
T13740 |
pobj |
neurons,of |
R6431 |
T13742 |
T13743 |
punct |
(,arrowhead |
R6432 |
T13743 |
T13741 |
parataxis |
arrowhead,neurons |
R6433 |
T13744 |
T13745 |
compound |
lamina,externa |
R6434 |
T13745 |
T13743 |
dep |
externa,arrowhead |
R6435 |
T13746 |
T13745 |
compound |
granularis,externa |
R6436 |
T13747 |
T13748 |
punct |
(,corpuscularis |
R6437 |
T13748 |
T13745 |
parataxis |
corpuscularis,externa |
R6438 |
T13749 |
T13748 |
punct |
),corpuscularis |
R6439 |
T13750 |
T13743 |
punct |
", ",arrowhead |
R6440 |
T13751 |
T13743 |
punct |
),arrowhead |
R6441 |
T13752 |
T13738 |
cc |
and,in |
R6442 |
T13753 |
T13738 |
conj |
in,in |
R6443 |
T13754 |
T13755 |
det |
the,cytoplasm |
R6444 |
T13755 |
T13753 |
pobj |
cytoplasm,in |
R6445 |
T13756 |
T13755 |
cc |
and,cytoplasm |
R6446 |
T13757 |
T13755 |
conj |
nuclei,cytoplasm |
R6447 |
T13758 |
T13755 |
prep |
of,cytoplasm |
R6448 |
T13759 |
T13758 |
pobj |
astrocytes,of |
R6449 |
T13760 |
T13761 |
punct |
(,arrow |
R6450 |
T13761 |
T13759 |
parataxis |
arrow,astrocytes |
R6451 |
T13762 |
T13763 |
compound |
lamina,molecularis |
R6452 |
T13763 |
T13761 |
dep |
molecularis,arrow |
R6453 |
T13764 |
T13761 |
punct |
", ",arrow |
R6454 |
T13765 |
T13761 |
punct |
;,arrow |
R6455 |
T13766 |
T13767 |
npadvmod |
GFAP,confirmed |
R6456 |
T13767 |
T13761 |
amod |
confirmed,arrow |
R6457 |
T13768 |
T13767 |
punct |
-,confirmed |
R6458 |
T13769 |
T13761 |
punct |
),arrow |
R6459 |
T13770 |
T13726 |
punct |
.,shows |
R6460 |
T13772 |
T13773 |
punct |
(,G |
R6461 |
T13773 |
T13774 |
meta |
G,confirms |
R6462 |
T13775 |
T13773 |
punct |
),G |
R6463 |
T13776 |
T13777 |
amod |
Higher,magnification |
R6464 |
T13777 |
T13774 |
nsubj |
magnification,confirms |
R6465 |
T13778 |
T13777 |
prep |
of,magnification |
R6466 |
T13779 |
T13780 |
det |
the,neurons |
R6467 |
T13780 |
T13778 |
pobj |
neurons,of |
R6468 |
T13781 |
T13780 |
amod |
pyramidal,neurons |
R6469 |
T13782 |
T13780 |
prep |
in,neurons |
R6470 |
T13783 |
T13784 |
det |
the,hippocampus |
R6471 |
T13784 |
T13782 |
pobj |
hippocampus,in |
R6472 |
T13785 |
T13786 |
det |
the,presence |
R6473 |
T13786 |
T13774 |
dobj |
presence,confirms |
R6474 |
T13787 |
T13786 |
prep |
of,presence |
R6475 |
T13788 |
T13789 |
det |
the,protein |
R6476 |
T13789 |
T13787 |
pobj |
protein,of |
R6477 |
T13790 |
T13791 |
compound |
Annexin,A7 |
R6478 |
T13791 |
T13789 |
compound |
A7,protein |
R6479 |
T13792 |
T13786 |
prep |
in,presence |
R6480 |
T13793 |
T13794 |
det |
the,nucleus |
R6481 |
T13794 |
T13792 |
pobj |
nucleus,in |
R6482 |
T13795 |
T13796 |
punct |
(,arrowhead |
R6483 |
T13796 |
T13794 |
parataxis |
arrowhead,nucleus |
R6484 |
T13797 |
T13796 |
punct |
),arrowhead |
R6485 |
T13798 |
T13794 |
prep |
of,nucleus |
R6486 |
T13799 |
T13800 |
amod |
mature,neurons |
R6487 |
T13800 |
T13798 |
pobj |
neurons,of |
R6488 |
T13801 |
T13774 |
punct |
.,confirms |
R6489 |
T13803 |
T13804 |
punct |
(,H |
R6490 |
T13804 |
T13805 |
meta |
H,stained |
R6491 |
T13806 |
T13804 |
punct |
),H |
R6492 |
T13807 |
T13808 |
aux |
To,confirm |
R6493 |
T13808 |
T13805 |
advcl |
confirm,stained |
R6494 |
T13809 |
T13808 |
advmod |
further,confirm |
R6495 |
T13810 |
T13808 |
dobj |
this,confirm |
R6496 |
T13811 |
T13805 |
punct |
", ",stained |
R6497 |
T13812 |
T13813 |
det |
a,section |
R6498 |
T13813 |
T13805 |
nsubjpass |
section,stained |
R6499 |
T13814 |
T13813 |
amod |
similar,section |
R6500 |
T13815 |
T13813 |
acl |
derived,section |
R6501 |
T13816 |
T13815 |
prep |
from,derived |
R6502 |
T13817 |
T13818 |
det |
an,mouse |
R6503 |
T13818 |
T13816 |
pobj |
mouse,from |
R6504 |
T13819 |
T13818 |
nmod |
AnxA7,mouse |
R6505 |
T13820 |
T13819 |
punct |
-,AnxA7 |
R6506 |
T13821 |
T13819 |
punct |
/,AnxA7 |
R6507 |
T13822 |
T13819 |
punct |
-,AnxA7 |
R6508 |
T13823 |
T13805 |
auxpass |
was,stained |
R6509 |
T13824 |
T13805 |
prep |
with,stained |
R6510 |
T13825 |
T13826 |
det |
the,antibody |
R6511 |
T13826 |
T13824 |
pobj |
antibody,with |
R6512 |
T13827 |
T13828 |
npadvmod |
annexin,specific |
R6513 |
T13828 |
T13826 |
amod |
specific,antibody |
R6514 |
T13829 |
T13805 |
cc |
and,stained |
R6515 |
T13830 |
T13805 |
conj |
lacked,stained |
R6516 |
T13831 |
T13832 |
det |
the,signal |
R6517 |
T13832 |
T13830 |
dobj |
signal,lacked |
R6518 |
T13833 |
T13832 |
amod |
nuclear,signal |
R6519 |
T13834 |
T13805 |
punct |
.,stained |
R6520 |
T13836 |
T13837 |
det |
The,stain |
R6521 |
T13837 |
T13839 |
nsubj |
stain,is |
R6522 |
T13838 |
T13837 |
amod |
residual,stain |
R6523 |
T13840 |
T13837 |
prep |
of,stain |
R6524 |
T13841 |
T13842 |
det |
the,tissue |
R6525 |
T13842 |
T13840 |
pobj |
tissue,of |
R6526 |
T13843 |
T13839 |
acomp |
unspecific,is |
R6527 |
T13844 |
T13839 |
punct |
", ",is |
R6528 |
T13845 |
T13846 |
mark |
as,observed |
R6529 |
T13846 |
T13839 |
advcl |
observed,is |
R6530 |
T13847 |
T13846 |
nsubjpass |
it,observed |
R6531 |
T13848 |
T13846 |
auxpass |
is,observed |
R6532 |
T13849 |
T13846 |
advmod |
also,observed |
R6533 |
T13850 |
T13846 |
prep |
in,observed |
R6534 |
T13851 |
T13850 |
pobj |
controls,in |
R6535 |
T13852 |
T13851 |
prep |
of,controls |
R6536 |
T13853 |
T13854 |
det |
the,brain |
R6537 |
T13854 |
T13852 |
pobj |
brain,of |
R6538 |
T13855 |
T13854 |
nmod |
AnxA7,brain |
R6539 |
T13856 |
T13855 |
punct |
-,AnxA7 |
R6540 |
T13857 |
T13855 |
punct |
/,AnxA7 |
R6541 |
T13858 |
T13855 |
punct |
-,AnxA7 |
R6542 |
T13859 |
T13846 |
advcl |
omitting,observed |
R6543 |
T13860 |
T13861 |
det |
the,antibody |
R6544 |
T13861 |
T13859 |
dobj |
antibody,omitting |
R6545 |
T13862 |
T13861 |
amod |
primary,antibody |
R6546 |
T13863 |
T13864 |
punct |
(,shown |
R6547 |
T13864 |
T13839 |
parataxis |
shown,is |
R6548 |
T13865 |
T13864 |
nsubj |
data,shown |
R6549 |
T13866 |
T13864 |
neg |
not,shown |
R6550 |
T13867 |
T13864 |
punct |
),shown |
R6551 |
T13868 |
T13839 |
punct |
.,is |
R6552 |
T13870 |
T13871 |
det |
All,sections |
R6553 |
T13871 |
T13873 |
nsubjpass |
sections,stained |
R6554 |
T13872 |
T13871 |
compound |
paraffin,sections |
R6555 |
T13874 |
T13873 |
auxpass |
were,stained |
R6556 |
T13875 |
T13873 |
prep |
with,stained |
R6557 |
T13876 |
T13877 |
nmod |
mAb,203 |
R6558 |
T13877 |
T13875 |
pobj |
203,with |
R6559 |
T13878 |
T13879 |
punct |
–,217 |
R6560 |
T13879 |
T13877 |
prep |
217,203 |
R6561 |
T13880 |
T13881 |
punct |
(,G |
R6562 |
T13881 |
T13877 |
parataxis |
G,203 |
R6563 |
T13882 |
T13881 |
nmod |
A,G |
R6564 |
T13883 |
T13881 |
punct |
", ",G |
R6565 |
T13884 |
T13881 |
nmod |
C,G |
R6566 |
T13885 |
T13881 |
punct |
", ",G |
R6567 |
T13886 |
T13881 |
nmod |
E,G |
R6568 |
T13887 |
T13881 |
punct |
", ",G |
R6569 |
T13888 |
T13881 |
nmod |
F,G |
R6570 |
T13889 |
T13881 |
punct |
", ",G |
R6571 |
T13890 |
T13881 |
punct |
),G |
R6572 |
T13891 |
T13877 |
cc |
or,203 |
R6573 |
T13892 |
T13893 |
amod |
anti-GFAP,antibody |
R6574 |
T13893 |
T13877 |
conj |
antibody,203 |
R6575 |
T13894 |
T13893 |
punct |
-,antibody |
R6576 |
T13895 |
T13896 |
punct |
(,B |
R6577 |
T13896 |
T13893 |
parataxis |
B,antibody |
R6578 |
T13897 |
T13896 |
punct |
),B |
R6579 |
T13898 |
T13873 |
punct |
.,stained |
R6580 |
T13900 |
T13901 |
det |
The,section |
R6581 |
T13901 |
T13904 |
nsubj |
section,lacks |
R6582 |
T13902 |
T13901 |
amod |
hippocampal,section |
R6583 |
T13903 |
T13901 |
compound |
control,section |
R6584 |
T13905 |
T13906 |
punct |
(,D |
R6585 |
T13906 |
T13901 |
parataxis |
D,section |
R6586 |
T13907 |
T13906 |
punct |
),D |
R6587 |
T13908 |
T13909 |
det |
the,antibody |
R6588 |
T13909 |
T13904 |
dobj |
antibody,lacks |
R6589 |
T13910 |
T13909 |
amod |
primary,antibody |
R6590 |
T13911 |
T13904 |
punct |
.,lacks |
R6597 |
T14188 |
T14189 |
compound |
Annexin,A7 |
R6598 |
T14189 |
T14190 |
compound |
A7,immunostaining |
R6599 |
T14191 |
T14190 |
prep |
in,immunostaining |
R6600 |
T14192 |
T14193 |
det |
the,cerebellum |
R6601 |
T14193 |
T14191 |
pobj |
cerebellum,in |
R6602 |
T14194 |
T14193 |
prep |
of,cerebellum |
R6603 |
T14195 |
T14196 |
amod |
adult,mice |
R6604 |
T14196 |
T14194 |
pobj |
mice,of |
R6605 |
T14197 |
T14190 |
punct |
.,immunostaining |
R6606 |
T14199 |
T14200 |
punct |
(,A |
R6607 |
T14200 |
T14201 |
meta |
A,presents |
R6608 |
T14202 |
T14200 |
punct |
),A |
R6609 |
T14203 |
T14204 |
amod |
Low,magnification |
R6610 |
T14204 |
T14201 |
nsubj |
magnification,presents |
R6611 |
T14205 |
T14204 |
prep |
of,magnification |
R6612 |
T14206 |
T14207 |
det |
the,cerebellum |
R6613 |
T14207 |
T14205 |
pobj |
cerebellum,of |
R6614 |
T14208 |
T14209 |
det |
an,expression |
R6615 |
T14209 |
T14201 |
dobj |
expression,presents |
R6616 |
T14210 |
T14211 |
compound |
Annexin,A7 |
R6617 |
T14211 |
T14209 |
compound |
A7,expression |
R6618 |
T14212 |
T14213 |
advmod |
mainly,in |
R6619 |
T14213 |
T14201 |
prep |
in,presents |
R6620 |
T14214 |
T14213 |
pobj |
cells,in |
R6621 |
T14215 |
T14214 |
prep |
of,cells |
R6622 |
T14216 |
T14217 |
det |
the,granulosum |
R6623 |
T14217 |
T14215 |
pobj |
granulosum,of |
R6624 |
T14218 |
T14217 |
compound |
stratum,granulosum |
R6625 |
T14219 |
T14217 |
cc |
and,granulosum |
R6626 |
T14220 |
T14221 |
compound |
laminae,medullares |
R6627 |
T14221 |
T14217 |
conj |
medullares,granulosum |
R6628 |
T14222 |
T14201 |
punct |
.,presents |
R6629 |
T14224 |
T14225 |
punct |
(,B |
R6630 |
T14225 |
T14226 |
meta |
B,section |
R6631 |
T14227 |
T14225 |
punct |
),B |
R6632 |
T14228 |
T14226 |
amod |
Corresponding,section |
R6633 |
T14229 |
T14226 |
acl |
stained,section |
R6634 |
T14230 |
T14229 |
prep |
for,stained |
R6635 |
T14231 |
T14230 |
pobj |
GFAP,for |
R6636 |
T14232 |
T14226 |
punct |
.,section |
R6637 |
T14234 |
T14235 |
punct |
(,C |
R6638 |
T14235 |
T14236 |
meta |
C,magnification |
R6639 |
T14237 |
T14235 |
punct |
),C |
R6640 |
T14238 |
T14236 |
amod |
Higher,magnification |
R6641 |
T14239 |
T14236 |
prep |
of,magnification |
R6642 |
T14240 |
T14239 |
pobj |
folia,of |
R6643 |
T14241 |
T14240 |
prep |
of,folia |
R6644 |
T14242 |
T14243 |
det |
the,cerebellum |
R6645 |
T14243 |
T14241 |
pobj |
cerebellum,of |
R6646 |
T14244 |
T14240 |
punct |
", ",folia |
R6647 |
T14245 |
T14246 |
advmod |
where,used |
R6648 |
T14246 |
T14240 |
relcl |
used,folia |
R6649 |
T14247 |
T14248 |
det |
a,antibody |
R6650 |
T14248 |
T14246 |
nsubjpass |
antibody,used |
R6651 |
T14249 |
T14248 |
amod |
polyclonal,antibody |
R6652 |
T14250 |
T14248 |
amod |
anti-AnnexinA7,antibody |
R6653 |
T14251 |
T14246 |
auxpass |
was,used |
R6654 |
T14252 |
T14236 |
punct |
;,magnification |
R6655 |
T14253 |
T14254 |
dep |
square,given |
R6656 |
T14254 |
T14236 |
dep |
given,magnification |
R6657 |
T14255 |
T14254 |
punct |
", ",given |
R6658 |
T14256 |
T14257 |
det |
a,magnification |
R6659 |
T14257 |
T14254 |
nsubjpass |
magnification,given |
R6660 |
T14258 |
T14257 |
amod |
higher,magnification |
R6661 |
T14259 |
T14257 |
prep |
of,magnification |
R6662 |
T14260 |
T14261 |
amod |
acorresponding,area |
R6663 |
T14261 |
T14259 |
pobj |
area,of |
R6664 |
T14262 |
T14261 |
acl |
stained,area |
R6665 |
T14263 |
T14262 |
prep |
with,stained |
R6666 |
T14264 |
T14265 |
nmod |
mAb,203 |
R6667 |
T14265 |
T14263 |
pobj |
203,with |
R6668 |
T14266 |
T14267 |
punct |
–,217 |
R6669 |
T14267 |
T14265 |
prep |
217,203 |
R6670 |
T14268 |
T14254 |
auxpass |
is,given |
R6671 |
T14269 |
T14254 |
prep |
in,given |
R6672 |
T14270 |
T14269 |
punct |
(,in |
R6673 |
T14271 |
T14269 |
pobj |
D,in |
R6674 |
T14272 |
T14236 |
punct |
),magnification |
R6675 |
T14273 |
T14236 |
punct |
.,magnification |
R6676 |
T14275 |
T14276 |
prep |
Between,observed |
R6677 |
T14277 |
T14278 |
compound |
stratum,granulosum |
R6678 |
T14278 |
T14275 |
pobj |
granulosum,Between |
R6679 |
T14279 |
T14278 |
cc |
and,granulosum |
R6680 |
T14280 |
T14281 |
compound |
stratum,moleculare |
R6681 |
T14281 |
T14278 |
conj |
moleculare,granulosum |
R6682 |
T14282 |
T14283 |
det |
the,layer |
R6683 |
T14283 |
T14276 |
nsubjpass |
layer,observed |
R6684 |
T14284 |
T14283 |
prep |
of,layer |
R6685 |
T14285 |
T14286 |
compound |
Purkinje,cells |
R6686 |
T14286 |
T14284 |
pobj |
cells,of |
R6687 |
T14287 |
T14286 |
punct |
-,cells |
R6688 |
T14288 |
T14289 |
punct |
(,piriformium |
R6689 |
T14289 |
T14286 |
parataxis |
piriformium,cells |
R6690 |
T14290 |
T14289 |
compound |
stratum,piriformium |
R6691 |
T14291 |
T14289 |
compound |
neuronorum,piriformium |
R6692 |
T14292 |
T14293 |
punct |
(,ganglionare |
R6693 |
T14293 |
T14289 |
parataxis |
ganglionare,piriformium |
R6694 |
T14294 |
T14293 |
punct |
),ganglionare |
R6695 |
T14295 |
T14289 |
punct |
),piriformium |
R6696 |
T14296 |
T14276 |
aux |
can,observed |
R6697 |
T14297 |
T14276 |
auxpass |
be,observed |
R6698 |
T14298 |
T14276 |
punct |
.,observed |
R6699 |
T14300 |
T14301 |
punct |
(,D |
R6700 |
T14301 |
T14302 |
meta |
D,Staining |
R6701 |
T14303 |
T14301 |
punct |
),D |
R6702 |
T14304 |
T14302 |
prep |
of,Staining |
R6703 |
T14305 |
T14306 |
det |
the,band |
R6704 |
T14306 |
T14304 |
pobj |
band,of |
R6705 |
T14307 |
T14306 |
prep |
of,band |
R6706 |
T14308 |
T14309 |
compound |
Purkinje,cells |
R6707 |
T14309 |
T14307 |
pobj |
cells,of |
R6708 |
T14310 |
T14309 |
punct |
-,cells |
R6709 |
T14311 |
T14312 |
punct |
(,arrows |
R6710 |
T14312 |
T14302 |
parataxis |
arrows,Staining |
R6711 |
T14313 |
T14312 |
punct |
),arrows |
R6712 |
T14314 |
T14302 |
punct |
.,Staining |
R6713 |
T14316 |
T14317 |
det |
The,stain |
R6714 |
T14317 |
T14322 |
nsubj |
stain,is |
R6715 |
T14318 |
T14317 |
amod |
positive,stain |
R6716 |
T14319 |
T14320 |
compound |
Annexin,A7 |
R6717 |
T14320 |
T14317 |
compound |
A7,stain |
R6718 |
T14321 |
T14317 |
punct |
-,stain |
R6719 |
T14323 |
T14317 |
prep |
in,stain |
R6720 |
T14324 |
T14325 |
det |
the,granulosum |
R6721 |
T14325 |
T14323 |
pobj |
granulosum,in |
R6722 |
T14326 |
T14325 |
compound |
stratum,granulosum |
R6723 |
T14327 |
T14322 |
prep |
due,is |
R6724 |
T14328 |
T14327 |
pcomp |
to,due |
R6725 |
T14329 |
T14327 |
pobj |
staining,due |
R6726 |
T14330 |
T14329 |
prep |
of,staining |
R6727 |
T14331 |
T14332 |
det |
the,nuclei |
R6728 |
T14332 |
T14330 |
pobj |
nuclei,of |
R6729 |
T14333 |
T14332 |
prep |
of,nuclei |
R6730 |
T14334 |
T14333 |
pobj |
neurons,of |
R6731 |
T14335 |
T14322 |
punct |
.,is |
R6732 |
T14337 |
T14338 |
punct |
(,E |
R6733 |
T14338 |
T14339 |
meta |
E,is |
R6734 |
T14340 |
T14338 |
punct |
),E |
R6735 |
T14341 |
T14339 |
prep |
In,is |
R6736 |
T14342 |
T14341 |
pobj |
addition,In |
R6737 |
T14343 |
T14342 |
prep |
to,addition |
R6738 |
T14344 |
T14345 |
det |
an,staining |
R6739 |
T14345 |
T14343 |
pobj |
staining,to |
R6740 |
T14346 |
T14345 |
amod |
intense,staining |
R6741 |
T14347 |
T14345 |
prep |
of,staining |
R6742 |
T14348 |
T14349 |
det |
the,nucleus |
R6743 |
T14349 |
T14347 |
pobj |
nucleus,of |
R6744 |
T14350 |
T14339 |
punct |
", ",is |
R6745 |
T14351 |
T14352 |
det |
the,body |
R6746 |
T14352 |
T14339 |
nsubj |
body,is |
R6747 |
T14353 |
T14352 |
compound |
cell,body |
R6748 |
T14354 |
T14355 |
npadvmod |
AnnexinA7,positive |
R6749 |
T14355 |
T14339 |
acomp |
positive,is |
R6750 |
T14356 |
T14355 |
punct |
-,positive |
R6751 |
T14357 |
T14339 |
prep |
including,is |
R6752 |
T14358 |
T14359 |
det |
both,dendrites |
R6753 |
T14359 |
T14357 |
pobj |
dendrites,including |
R6754 |
T14360 |
T14359 |
prep |
of,dendrites |
R6755 |
T14361 |
T14362 |
det |
the,cell |
R6756 |
T14362 |
T14360 |
pobj |
cell,of |
R6757 |
T14363 |
T14362 |
compound |
Purkinje,cell |
R6758 |
T14364 |
T14362 |
punct |
-,cell |
R6759 |
T14365 |
T14362 |
acl |
shown,cell |
R6760 |
T14366 |
T14339 |
punct |
.,is |
R6761 |
T14368 |
T14369 |
punct |
(,F |
R6762 |
T14369 |
T14370 |
meta |
F,section |
R6763 |
T14371 |
T14369 |
punct |
),F |
R6764 |
T14372 |
T14370 |
amod |
Corresponding,section |
R6765 |
T14373 |
T14370 |
prep |
from,section |
R6766 |
T14374 |
T14375 |
det |
an,mouse |
R6767 |
T14375 |
T14373 |
pobj |
mouse,from |
R6768 |
T14376 |
T14375 |
nmod |
AnxA7,mouse |
R6769 |
T14377 |
T14376 |
punct |
-,AnxA7 |
R6770 |
T14378 |
T14376 |
punct |
/,AnxA7 |
R6771 |
T14379 |
T14376 |
punct |
-,AnxA7 |
R6772 |
T14380 |
T14370 |
punct |
.,section |
R6773 |
T14382 |
T14383 |
punct |
(,G |
R6774 |
T14383 |
T14384 |
meta |
G,staining |
R6775 |
T14385 |
T14383 |
punct |
),G |
R6776 |
T14386 |
T14384 |
compound |
AnnexinA7,staining |
R6777 |
T14387 |
T14384 |
prep |
of,staining |
R6778 |
T14388 |
T14387 |
pobj |
axons,of |
R6779 |
T14389 |
T14390 |
punct |
(,arrowheads |
R6780 |
T14390 |
T14388 |
parataxis |
arrowheads,axons |
R6781 |
T14391 |
T14390 |
punct |
),arrowheads |
R6782 |
T14392 |
T14388 |
acl |
running,axons |
R6783 |
T14393 |
T14392 |
prep |
from,running |
R6784 |
T14394 |
T14395 |
det |
the,medullares |
R6785 |
T14395 |
T14393 |
pobj |
medullares,from |
R6786 |
T14396 |
T14395 |
compound |
laminae,medullares |
R6787 |
T14397 |
T14392 |
prep |
to,running |
R6788 |
T14398 |
T14399 |
det |
the,layer |
R6789 |
T14399 |
T14397 |
pobj |
layer,to |
R6790 |
T14400 |
T14401 |
compound |
Purkinje,cell |
R6791 |
T14401 |
T14399 |
compound |
cell,layer |
R6792 |
T14402 |
T14401 |
punct |
-,cell |
R6793 |
T14403 |
T14399 |
acl |
located,layer |
R6794 |
T14404 |
T14403 |
prep |
in,located |
R6795 |
T14405 |
T14406 |
det |
the,end |
R6796 |
T14406 |
T14404 |
pobj |
end,in |
R6797 |
T14407 |
T14406 |
amod |
round,end |
R6798 |
T14408 |
T14406 |
prep |
of,end |
R6799 |
T14409 |
T14410 |
det |
a,convolution |
R6800 |
T14410 |
T14408 |
pobj |
convolution,of |
R6801 |
T14411 |
T14384 |
punct |
.,staining |
R6802 |
T14413 |
T14414 |
nmod |
Sections,G |
R6803 |
T14414 |
T14423 |
nsubjpass |
G,stained |
R6804 |
T14415 |
T14414 |
nmod |
A,G |
R6805 |
T14416 |
T14414 |
punct |
", ",G |
R6806 |
T14417 |
T14414 |
nmod |
D,G |
R6807 |
T14418 |
T14414 |
punct |
", ",G |
R6808 |
T14419 |
T14414 |
nmod |
E,G |
R6809 |
T14420 |
T14414 |
punct |
", ",G |
R6810 |
T14421 |
T14414 |
nmod |
F,G |
R6811 |
T14422 |
T14414 |
punct |
", ",G |
R6812 |
T14424 |
T14423 |
auxpass |
were,stained |
R6813 |
T14425 |
T14423 |
prep |
with,stained |
R6814 |
T14426 |
T14427 |
nmod |
mAb,203 |
R6815 |
T14427 |
T14425 |
pobj |
203,with |
R6816 |
T14428 |
T14429 |
punct |
–,217 |
R6817 |
T14429 |
T14427 |
prep |
217,203 |
R6818 |
T14430 |
T14423 |
punct |
.,stained |
R6819 |
T14500 |
T14501 |
compound |
Annexin,A7 |
R6820 |
T14501 |
T14502 |
compound |
A7,immunostaining |
R6821 |
T14503 |
T14502 |
prep |
in,immunostaining |
R6822 |
T14504 |
T14505 |
amod |
human,isocortex |
R6823 |
T14505 |
T14503 |
pobj |
isocortex,in |
R6824 |
T14506 |
T14502 |
punct |
.,immunostaining |
R6825 |
T14508 |
T14509 |
punct |
(,A |
R6826 |
T14509 |
T14510 |
meta |
A,labeled |
R6827 |
T14511 |
T14509 |
punct |
),A |
R6828 |
T14512 |
T14513 |
amod |
Pyramidal,neurons |
R6829 |
T14513 |
T14510 |
nsubjpass |
neurons,labeled |
R6830 |
T14514 |
T14515 |
punct |
(,arrow |
R6831 |
T14515 |
T14513 |
parataxis |
arrow,neurons |
R6832 |
T14516 |
T14515 |
amod |
bold,arrow |
R6833 |
T14517 |
T14515 |
punct |
),arrow |
R6834 |
T14518 |
T14513 |
cc |
and,neurons |
R6835 |
T14519 |
T14520 |
amod |
apical,dendrites |
R6836 |
T14520 |
T14513 |
conj |
dendrites,neurons |
R6837 |
T14521 |
T14522 |
punct |
(,arrows |
R6838 |
T14522 |
T14520 |
parataxis |
arrows,dendrites |
R6839 |
T14523 |
T14522 |
amod |
small,arrows |
R6840 |
T14524 |
T14522 |
punct |
),arrows |
R6841 |
T14525 |
T14510 |
auxpass |
were,labeled |
R6842 |
T14526 |
T14510 |
advmod |
clearly,labeled |
R6843 |
T14527 |
T14510 |
prep |
with,labeled |
R6844 |
T14528 |
T14529 |
det |
the,antibody |
R6845 |
T14529 |
T14527 |
pobj |
antibody,with |
R6846 |
T14530 |
T14529 |
prep |
against,antibody |
R6847 |
T14531 |
T14532 |
compound |
Annexin,A7 |
R6848 |
T14532 |
T14530 |
pobj |
A7,against |
R6849 |
T14533 |
T14510 |
prep |
in,labeled |
R6850 |
T14534 |
T14535 |
det |
the,cortex |
R6851 |
T14535 |
T14533 |
pobj |
cortex,in |
R6852 |
T14536 |
T14535 |
amod |
human,cortex |
R6853 |
T14537 |
T14535 |
amod |
parietal,cortex |
R6854 |
T14538 |
T14510 |
punct |
.,labeled |
R6855 |
T14540 |
T14541 |
punct |
(,B |
R6856 |
T14541 |
T14542 |
meta |
B,seen |
R6857 |
T14543 |
T14541 |
punct |
),B |
R6858 |
T14544 |
T14545 |
det |
A,staining |
R6859 |
T14545 |
T14542 |
nsubjpass |
staining,seen |
R6860 |
T14546 |
T14545 |
amod |
dendritic,staining |
R6861 |
T14547 |
T14542 |
auxpass |
was,seen |
R6862 |
T14548 |
T14542 |
advmod |
also,seen |
R6863 |
T14549 |
T14542 |
prep |
in,seen |
R6864 |
T14550 |
T14551 |
det |
the,layer |
R6865 |
T14551 |
T14549 |
pobj |
layer,in |
R6866 |
T14552 |
T14551 |
amod |
molecular,layer |
R6867 |
T14553 |
T14554 |
punct |
(,arrows |
R6868 |
T14554 |
T14542 |
parataxis |
arrows,seen |
R6869 |
T14555 |
T14554 |
amod |
small,arrows |
R6870 |
T14556 |
T14554 |
punct |
),arrows |
R6871 |
T14557 |
T14542 |
punct |
.,seen |
R6872 |
T14559 |
T14560 |
prep |
In,labeled |
R6873 |
T14561 |
T14559 |
pobj |
addition,In |
R6874 |
T14562 |
T14563 |
det |
the,astrocytes |
R6875 |
T14563 |
T14560 |
nsubjpass |
astrocytes,labeled |
R6876 |
T14564 |
T14563 |
amod |
subpial,astrocytes |
R6877 |
T14565 |
T14560 |
auxpass |
were,labeled |
R6878 |
T14566 |
T14560 |
advmod |
weakly,labeled |
R6879 |
T14567 |
T14568 |
punct |
(,arrows |
R6880 |
T14568 |
T14560 |
parataxis |
arrows,labeled |
R6881 |
T14569 |
T14570 |
amod |
double,headed |
R6882 |
T14570 |
T14568 |
amod |
headed,arrows |
R6883 |
T14571 |
T14568 |
punct |
),arrows |
R6884 |
T14572 |
T14560 |
punct |
.,labeled |
R6885 |
T14574 |
T14575 |
det |
The,staining |
R6886 |
T14575 |
T14576 |
nsubj |
staining,was |
R6887 |
T14576 |
T14577 |
ccomp |
was,showed |
R6888 |
T14578 |
T14576 |
acomp |
cytoplasmic,was |
R6889 |
T14579 |
T14577 |
punct |
", ",showed |
R6890 |
T14580 |
T14581 |
advmod |
only,astrocytes |
R6891 |
T14581 |
T14577 |
nsubj |
astrocytes,showed |
R6892 |
T14582 |
T14581 |
amod |
few,astrocytes |
R6893 |
T14583 |
T14584 |
det |
a,staining |
R6894 |
T14584 |
T14577 |
dobj |
staining,showed |
R6895 |
T14585 |
T14584 |
amod |
nuclear,staining |
R6896 |
T14586 |
T14587 |
advmod |
as,well |
R6897 |
T14587 |
T14577 |
advmod |
well,showed |
R6898 |
T14588 |
T14589 |
punct |
(,inset |
R6899 |
T14589 |
T14577 |
parataxis |
inset,showed |
R6900 |
T14590 |
T14589 |
punct |
),inset |
R6901 |
T14591 |
T14577 |
punct |
.,showed |
R6902 |
T14593 |
T14594 |
punct |
(,C |
R6903 |
T14594 |
T14595 |
meta |
C,seen |
R6904 |
T14596 |
T14594 |
punct |
),C |
R6905 |
T14597 |
T14595 |
dep |
Enlargement,seen |
R6906 |
T14598 |
T14597 |
prep |
of,Enlargement |
R6907 |
T14599 |
T14598 |
punct |
(,of |
R6908 |
T14600 |
T14598 |
pobj |
A,of |
R6909 |
T14601 |
T14595 |
punct |
),seen |
R6910 |
T14602 |
T14595 |
punct |
: ,seen |
R6911 |
T14603 |
T14604 |
compound |
Annexin,A7 |
R6912 |
T14604 |
T14595 |
nsubjpass |
A7,seen |
R6913 |
T14605 |
T14595 |
auxpass |
was,seen |
R6914 |
T14606 |
T14595 |
prep |
at,seen |
R6915 |
T14607 |
T14608 |
det |
the,membrane |
R6916 |
T14608 |
T14606 |
pobj |
membrane,at |
R6917 |
T14609 |
T14608 |
compound |
cell,membrane |
R6918 |
T14610 |
T14608 |
prep |
of,membrane |
R6919 |
T14611 |
T14612 |
det |
the,perikaryon |
R6920 |
T14612 |
T14610 |
pobj |
perikaryon,of |
R6921 |
T14613 |
T14614 |
punct |
(,arrow |
R6922 |
T14614 |
T14612 |
parataxis |
arrow,perikaryon |
R6923 |
T14615 |
T14614 |
amod |
bold,arrow |
R6924 |
T14616 |
T14614 |
punct |
),arrow |
R6925 |
T14617 |
T14612 |
cc |
and,perikaryon |
R6926 |
T14618 |
T14619 |
det |
the,dendrite |
R6927 |
T14619 |
T14612 |
conj |
dendrite,perikaryon |
R6928 |
T14620 |
T14619 |
amod |
apical,dendrite |
R6929 |
T14621 |
T14622 |
punct |
(,arrows |
R6930 |
T14622 |
T14619 |
parataxis |
arrows,dendrite |
R6931 |
T14623 |
T14622 |
amod |
small,arrows |
R6932 |
T14624 |
T14622 |
punct |
),arrows |
R6933 |
T14625 |
T14595 |
punct |
.,seen |
R6934 |
T14627 |
T14628 |
punct |
(,D |
R6935 |
T14628 |
T14629 |
meta |
D,show |
R6936 |
T14630 |
T14628 |
punct |
),D |
R6937 |
T14631 |
T14632 |
det |
A,control |
R6938 |
T14632 |
T14629 |
nsubj |
control,show |
R6939 |
T14633 |
T14632 |
amod |
blank,control |
R6940 |
T14634 |
T14632 |
acl |
lacking,control |
R6941 |
T14635 |
T14636 |
det |
the,antibody |
R6942 |
T14636 |
T14634 |
dobj |
antibody,lacking |
R6943 |
T14637 |
T14636 |
amod |
primary,antibody |
R6944 |
T14638 |
T14629 |
aux |
did,show |
R6945 |
T14639 |
T14629 |
neg |
not,show |
R6946 |
T14640 |
T14641 |
det |
a,labeling |
R6947 |
T14641 |
T14629 |
dobj |
labeling,show |
R6948 |
T14642 |
T14641 |
amod |
specific,labeling |
R6949 |
T14643 |
T14629 |
punct |
", ",show |
R6950 |
T14644 |
T14629 |
cc |
but,show |
R6951 |
T14645 |
T14646 |
amod |
autofluorescent,lipofuscin |
R6952 |
T14646 |
T14647 |
nsubj |
lipofuscin,was |
R6953 |
T14647 |
T14629 |
conj |
was,show |
R6954 |
T14648 |
T14647 |
acomp |
detectable,was |
R6955 |
T14649 |
T14647 |
prep |
in,was |
R6956 |
T14650 |
T14651 |
det |
the,neurons |
R6957 |
T14651 |
T14649 |
pobj |
neurons,in |
R6958 |
T14652 |
T14653 |
punct |
(,arrowheads |
R6959 |
T14653 |
T14647 |
parataxis |
arrowheads,was |
R6960 |
T14654 |
T14653 |
punct |
),arrowheads |
R6961 |
T14655 |
T14647 |
punct |
.,was |
R6962 |
T14658 |
T14657 |
punct |
", ",Bar |
R6963 |
T14659 |
T14660 |
punct |
(,A |
R6964 |
T14660 |
T14661 |
meta |
A,μm |
R6965 |
T14661 |
T14657 |
appos |
μm,Bar |
R6966 |
T14662 |
T14660 |
punct |
),A |
R6967 |
T14663 |
T14661 |
nummod |
70,μm |
R6968 |
T14664 |
T14661 |
punct |
", ",μm |
R6969 |
T14665 |
T14666 |
punct |
(,B |
R6970 |
T14666 |
T14667 |
meta |
B,μm |
R6971 |
T14667 |
T14661 |
appos |
μm,μm |
R6972 |
T14668 |
T14666 |
punct |
),B |
R6973 |
T14669 |
T14667 |
nummod |
50,μm |
R6974 |
T14670 |
T14661 |
punct |
", ",μm |
R6975 |
T14671 |
T14672 |
punct |
(,B |
R6976 |
T14672 |
T14673 |
meta |
B,μm |
R6977 |
T14673 |
T14661 |
appos |
μm,μm |
R6978 |
T14674 |
T14672 |
punct |
-,B |
R6979 |
T14675 |
T14672 |
dep |
inset,B |
R6980 |
T14676 |
T14672 |
punct |
", ",B |
R6981 |
T14677 |
T14672 |
dep |
C,B |
R6982 |
T14678 |
T14672 |
punct |
),B |
R6983 |
T14679 |
T14673 |
nummod |
17,μm |
R6984 |
T14680 |
T14661 |
punct |
", ",μm |
R6985 |
T14681 |
T14682 |
punct |
(,D |
R6986 |
T14682 |
T14683 |
meta |
D,μm |
R6987 |
T14683 |
T14661 |
appos |
μm,μm |
R6988 |
T14684 |
T14682 |
punct |
),D |
R6989 |
T14685 |
T14683 |
nummod |
20,μm |
R6990 |
T14686 |
T14657 |
punct |
.,Bar |
R6997 |
T14995 |
T14996 |
amod |
Nuclear,localization |
R6998 |
T14997 |
T14996 |
prep |
of,localization |
R6999 |
T14998 |
T14999 |
compound |
Annexin,A7 |
R7000 |
T14999 |
T14997 |
pobj |
A7,of |
R7001 |
T15000 |
T14996 |
punct |
.,localization |
R7002 |
T15002 |
T15003 |
punct |
(,A |
R7003 |
T15003 |
T15004 |
meta |
A,Extraction |
R7004 |
T15005 |
T15003 |
punct |
),A |
R7005 |
T15006 |
T15004 |
prep |
of,Extraction |
R7006 |
T15007 |
T15008 |
compound |
Annexin,A7 |
R7007 |
T15008 |
T15006 |
pobj |
A7,of |
R7008 |
T15009 |
T15004 |
prep |
from,Extraction |
R7009 |
T15010 |
T15009 |
pobj |
nuclei,from |
R7010 |
T15011 |
T15010 |
prep |
of,nuclei |
R7011 |
T15012 |
T15011 |
pobj |
neuronal,of |
R7012 |
T15013 |
T15014 |
punct |
(,PC |
R7013 |
T15014 |
T15012 |
parataxis |
PC,neuronal |
R7014 |
T15015 |
T15014 |
punct |
-,PC |
R7015 |
T15016 |
T15014 |
nummod |
12,PC |
R7016 |
T15017 |
T15014 |
punct |
", ",PC |
R7017 |
T15018 |
T15014 |
appos |
N2A,PC |
R7018 |
T15019 |
T15014 |
punct |
),PC |
R7019 |
T15020 |
T15012 |
cc |
and,neuronal |
R7020 |
T15021 |
T15022 |
det |
an,astroglial |
R7021 |
T15022 |
T15023 |
nmod |
astroglial,line |
R7022 |
T15023 |
T15012 |
conj |
line,neuronal |
R7023 |
T15024 |
T15025 |
punct |
(,C6 |
R7024 |
T15025 |
T15022 |
parataxis |
C6,astroglial |
R7025 |
T15026 |
T15025 |
punct |
),C6 |
R7026 |
T15027 |
T15023 |
compound |
cell,line |
R7027 |
T15028 |
T15004 |
acl |
using,Extraction |
R7028 |
T15029 |
T15030 |
det |
a,buffer |
R7029 |
T15030 |
T15028 |
dobj |
buffer,using |
R7030 |
T15031 |
T15030 |
amod |
hypotonic,buffer |
R7031 |
T15032 |
T15004 |
punct |
.,Extraction |
R7032 |
T15034 |
T15035 |
nsubjpass |
Samples,subjected |
R7033 |
T15036 |
T15034 |
prep |
of,Samples |
R7034 |
T15037 |
T15038 |
amod |
total,cells |
R7035 |
T15038 |
T15036 |
pobj |
cells,of |
R7036 |
T15039 |
T15040 |
punct |
(,c |
R7037 |
T15040 |
T15034 |
parataxis |
c,Samples |
R7038 |
T15041 |
T15040 |
punct |
),c |
R7039 |
T15042 |
T15034 |
punct |
", ",Samples |
R7040 |
T15043 |
T15034 |
cc |
and,Samples |
R7041 |
T15044 |
T15045 |
amod |
corresponding,amounts |
R7042 |
T15045 |
T15034 |
conj |
amounts,Samples |
R7043 |
T15046 |
T15045 |
prep |
of,amounts |
R7044 |
T15047 |
T15048 |
amod |
nuclear,membranes |
R7045 |
T15048 |
T15046 |
pobj |
membranes,of |
R7046 |
T15049 |
T15050 |
punct |
(,m |
R7047 |
T15050 |
T15048 |
parataxis |
m,membranes |
R7048 |
T15051 |
T15050 |
punct |
),m |
R7049 |
T15052 |
T15048 |
cc |
and,membranes |
R7050 |
T15053 |
T15048 |
conj |
nucleoplasm,membranes |
R7051 |
T15054 |
T15055 |
punct |
(,p |
R7052 |
T15055 |
T15053 |
parataxis |
p,nucleoplasm |
R7053 |
T15056 |
T15055 |
punct |
),p |
R7054 |
T15057 |
T15035 |
auxpass |
were,subjected |
R7055 |
T15058 |
T15035 |
prep |
to,subjected |
R7056 |
T15059 |
T15060 |
compound |
SDS,PAGE |
R7057 |
T15060 |
T15058 |
pobj |
PAGE,to |
R7058 |
T15061 |
T15060 |
punct |
-,PAGE |
R7059 |
T15062 |
T15060 |
cc |
and,PAGE |
R7060 |
T15063 |
T15064 |
compound |
Western,blotting |
R7061 |
T15064 |
T15060 |
conj |
blotting,PAGE |
R7062 |
T15065 |
T15035 |
punct |
.,subjected |
R7063 |
T15067 |
T15068 |
nmod |
Annexin,A7 |
R7064 |
T15068 |
T15069 |
nmod |
A7,isoforms |
R7065 |
T15069 |
T15073 |
nsubj |
isoforms,are |
R7066 |
T15070 |
T15068 |
punct |
(,A7 |
R7067 |
T15071 |
T15068 |
appos |
AnxA7,A7 |
R7068 |
T15072 |
T15069 |
punct |
),isoforms |
R7069 |
T15074 |
T15073 |
acomp |
extractable,are |
R7070 |
T15075 |
T15074 |
prep |
from,extractable |
R7071 |
T15076 |
T15077 |
det |
the,nucleus |
R7072 |
T15077 |
T15075 |
pobj |
nucleus,from |
R7073 |
T15078 |
T15077 |
prep |
of,nucleus |
R7074 |
T15079 |
T15080 |
det |
the,cells |
R7075 |
T15080 |
T15078 |
pobj |
cells,of |
R7076 |
T15081 |
T15080 |
amod |
indicated,cells |
R7077 |
T15082 |
T15083 |
punct |
(,arrowhead |
R7078 |
T15083 |
T15080 |
parataxis |
arrowhead,cells |
R7079 |
T15084 |
T15085 |
nummod |
47,kDa |
R7080 |
T15085 |
T15086 |
nmod |
kDa,isoform |
R7081 |
T15086 |
T15083 |
dep |
isoform,arrowhead |
R7082 |
T15087 |
T15085 |
cc |
and,kDa |
R7083 |
T15088 |
T15089 |
nummod |
51,kDa |
R7084 |
T15089 |
T15085 |
conj |
kDa,kDa |
R7085 |
T15090 |
T15083 |
punct |
", ",arrowhead |
R7086 |
T15091 |
T15083 |
punct |
),arrowhead |
R7087 |
T15092 |
T15073 |
punct |
.,are |
R7088 |
T15094 |
T15095 |
prep |
For,shown |
R7089 |
T15096 |
T15094 |
pobj |
control,For |
R7090 |
T15097 |
T15095 |
punct |
", ",shown |
R7091 |
T15098 |
T15095 |
nsubjpass |
immunoblotting,shown |
R7092 |
T15099 |
T15098 |
prep |
of,immunoblotting |
R7093 |
T15100 |
T15099 |
pobj |
LAP2α,of |
R7094 |
T15101 |
T15102 |
punct |
(,isoform |
R7095 |
T15102 |
T15100 |
parataxis |
isoform,LAP2α |
R7096 |
T15103 |
T15104 |
nummod |
75,kDa |
R7097 |
T15104 |
T15102 |
dep |
kDa,isoform |
R7098 |
T15105 |
T15102 |
punct |
;,isoform |
R7099 |
T15106 |
T15107 |
amod |
non-membrane,bound |
R7100 |
T15107 |
T15102 |
amod |
bound,isoform |
R7101 |
T15108 |
T15107 |
punct |
-,bound |
R7102 |
T15109 |
T15102 |
punct |
),isoform |
R7103 |
T15110 |
T15100 |
punct |
", ",LAP2α |
R7104 |
T15111 |
T15100 |
conj |
Emerin,LAP2α |
R7105 |
T15112 |
T15111 |
punct |
(,Emerin |
R7106 |
T15113 |
T15111 |
appos |
EM,Emerin |
R7107 |
T15114 |
T15115 |
punct |
", ",kDa |
R7108 |
T15115 |
T15111 |
parataxis |
kDa,Emerin |
R7109 |
T15116 |
T15115 |
nummod |
34,kDa |
R7110 |
T15117 |
T15115 |
punct |
),kDa |
R7111 |
T15118 |
T15111 |
punct |
", ",Emerin |
R7112 |
T15119 |
T15111 |
cc |
and,Emerin |
R7113 |
T15120 |
T15111 |
conj |
tubulin,Emerin |
R7114 |
T15121 |
T15120 |
punct |
(,tubulin |
R7115 |
T15122 |
T15120 |
appos |
TB,tubulin |
R7116 |
T15123 |
T15124 |
punct |
", ",kDa |
R7117 |
T15124 |
T15120 |
parataxis |
kDa,tubulin |
R7118 |
T15125 |
T15124 |
nummod |
55,kDa |
R7119 |
T15126 |
T15124 |
punct |
),kDa |
R7120 |
T15127 |
T15128 |
dep |
which,are |
R7121 |
T15128 |
T15100 |
relcl |
are,LAP2α |
R7122 |
T15129 |
T15128 |
acomp |
specific,are |
R7123 |
T15130 |
T15129 |
prep |
for,specific |
R7124 |
T15131 |
T15130 |
pobj |
nucleoplasm,for |
R7125 |
T15132 |
T15133 |
punct |
(,LAP2α |
R7126 |
T15133 |
T15131 |
parataxis |
LAP2α,nucleoplasm |
R7127 |
T15134 |
T15133 |
punct |
),LAP2α |
R7128 |
T15135 |
T15100 |
punct |
", ",LAP2α |
R7129 |
T15136 |
T15137 |
amod |
nuclear,membranes |
R7130 |
T15137 |
T15100 |
appos |
membranes,LAP2α |
R7131 |
T15138 |
T15139 |
punct |
(,Emerin |
R7132 |
T15139 |
T15137 |
parataxis |
Emerin,membranes |
R7133 |
T15140 |
T15139 |
punct |
),Emerin |
R7134 |
T15141 |
T15137 |
punct |
", ",membranes |
R7135 |
T15142 |
T15137 |
cc |
and,membranes |
R7136 |
T15143 |
T15144 |
det |
the,cytoplasm |
R7137 |
T15144 |
T15137 |
conj |
cytoplasm,membranes |
R7138 |
T15145 |
T15146 |
punct |
(,tubulin |
R7139 |
T15146 |
T15144 |
parataxis |
tubulin,cytoplasm |
R7140 |
T15147 |
T15146 |
punct |
),tubulin |
R7141 |
T15148 |
T15095 |
auxpass |
are,shown |
R7142 |
T15149 |
T15095 |
punct |
.,shown |
R7143 |
T15152 |
T15153 |
mark |
that,is |
R7144 |
T15153 |
T15151 |
ccomp |
is,Note |
R7145 |
T15154 |
T15155 |
det |
the,nucleoplasm |
R7146 |
T15155 |
T15153 |
nsubj |
nucleoplasm,is |
R7147 |
T15156 |
T15157 |
advmod |
only,partially |
R7148 |
T15157 |
T15158 |
advmod |
partially,extractable |
R7149 |
T15158 |
T15153 |
acomp |
extractable,is |
R7150 |
T15159 |
T15151 |
punct |
.,Note |
R7151 |
T15161 |
T15162 |
punct |
(,B |
R7152 |
T15162 |
T15163 |
meta |
B,images |
R7153 |
T15164 |
T15162 |
punct |
),B |
R7154 |
T15165 |
T15163 |
compound |
Immunofluorescence,images |
R7155 |
T15166 |
T15163 |
prep |
of,images |
R7156 |
T15167 |
T15168 |
det |
the,cells |
R7157 |
T15168 |
T15166 |
pobj |
cells,of |
R7158 |
T15169 |
T15168 |
nmod |
PC,cells |
R7159 |
T15170 |
T15169 |
punct |
-,PC |
R7160 |
T15171 |
T15169 |
nummod |
12,PC |
R7161 |
T15172 |
T15168 |
acl |
used,cells |
R7162 |
T15173 |
T15163 |
punct |
.,images |
R7163 |
T15175 |
T15176 |
nsubjpass |
Cells,fixed |
R7164 |
T15177 |
T15176 |
auxpass |
were,fixed |
R7165 |
T15178 |
T15176 |
prep |
with,fixed |
R7166 |
T15179 |
T15178 |
pobj |
paraformaldehyde,with |
R7167 |
T15180 |
T15176 |
punct |
", ",fixed |
R7168 |
T15181 |
T15176 |
conj |
permeabilized,fixed |
R7169 |
T15182 |
T15181 |
prep |
with,permeabilized |
R7170 |
T15183 |
T15184 |
compound |
Triton,X |
R7171 |
T15184 |
T15182 |
pobj |
X,with |
R7172 |
T15185 |
T15184 |
punct |
-,X |
R7173 |
T15186 |
T15184 |
nummod |
100,X |
R7174 |
T15187 |
T15181 |
punct |
", ",permeabilized |
R7175 |
T15188 |
T15181 |
conj |
pretreated,permeabilized |
R7176 |
T15189 |
T15188 |
prep |
with,pretreated |
R7177 |
T15190 |
T15189 |
pobj |
trypsin,with |
R7178 |
T15191 |
T15192 |
mark |
as,indicated |
R7179 |
T15192 |
T15188 |
advcl |
indicated,pretreated |
R7180 |
T15193 |
T15188 |
punct |
", ",pretreated |
R7181 |
T15194 |
T15188 |
cc |
and,pretreated |
R7182 |
T15195 |
T15188 |
conj |
stained,pretreated |
R7183 |
T15196 |
T15195 |
prep |
with,stained |
R7184 |
T15197 |
T15198 |
compound |
Annexin,A7 |
R7185 |
T15198 |
T15199 |
npadvmod |
A7,specific |
R7186 |
T15199 |
T15200 |
amod |
specific,203 |
R7187 |
T15200 |
T15196 |
pobj |
203,with |
R7188 |
T15201 |
T15200 |
nmod |
mAb,203 |
R7189 |
T15202 |
T15203 |
punct |
–,217 |
R7190 |
T15203 |
T15200 |
prep |
217,203 |
R7191 |
T15204 |
T15205 |
punct |
", ",μm |
R7192 |
T15205 |
T15195 |
parataxis |
μm,stained |
R7193 |
T15206 |
T15205 |
dep |
DAPI,μm |
R7194 |
T15207 |
T15206 |
prep |
in,DAPI |
R7195 |
T15208 |
T15207 |
amod |
blue,in |
R7196 |
T15209 |
T15205 |
punct |
", ",μm |
R7197 |
T15210 |
T15205 |
dep |
bar,μm |
R7198 |
T15211 |
T15205 |
nummod |
10,μm |
R7199 |
T15212 |
T15176 |
punct |
.,fixed |
R924 |
T2453 |
T2454 |
det |
the,level |
R925 |
T2454 |
T2451 |
pobj |
level,On |
R926 |
T2455 |
T2454 |
compound |
protein,level |
R927 |
T2456 |
T2452 |
punct |
", ",express |
R928 |
T2457 |
T2458 |
compound |
ES,cells |
R929 |
T2458 |
T2452 |
nsubj |
cells,express |
R930 |
T2459 |
T2460 |
advmod |
only,isoform |
R931 |
T2460 |
T2452 |
dobj |
isoform,express |
R932 |
T2461 |
T2460 |
det |
the,isoform |
R933 |
T2462 |
T2460 |
amod |
smaller,isoform |
R934 |
T2463 |
T2464 |
compound |
Annexin,A7 |
R935 |
T2464 |
T2460 |
compound |
A7,isoform |
R936 |
T2465 |
T2460 |
prep |
of,isoform |
R937 |
T2466 |
T2467 |
nummod |
47,kDa |
R938 |
T2467 |
T2465 |
pobj |
kDa,of |
R939 |
T2468 |
T2469 |
punct |
(,1B |
R940 |
T2469 |
T2452 |
parataxis |
1B,express |
R941 |
T2470 |
T2469 |
compound |
Fig.,1B |
R942 |
T2471 |
T2469 |
punct |
", ",1B |
R943 |
T2472 |
T2473 |
nmod |
neuro,cells |
R944 |
T2473 |
T2476 |
nsubjpass |
cells,included |
R945 |
T2474 |
T2472 |
punct |
-,neuro |
R946 |
T2475 |
T2472 |
nummod |
2a,neuro |
R947 |
T2476 |
T2469 |
ccomp |
included,1B |
R948 |
T2477 |
T2473 |
cc |
and,cells |
R949 |
T2478 |
T2479 |
compound |
heart,tissue |
R950 |
T2479 |
T2473 |
conj |
tissue,cells |
R951 |
T2480 |
T2476 |
auxpass |
are,included |
R952 |
T2481 |
T2476 |
prep |
for,included |
R953 |
T2482 |
T2481 |
pobj |
control,for |
R954 |
T2483 |
T2469 |
punct |
),1B |
R955 |
T2484 |
T2452 |
punct |
.,express |
R956 |
T2486 |
T2487 |
nsubj |
Immunohistochemistry,confirmed |
R957 |
T2488 |
T2489 |
det |
the,presence |
R958 |
T2489 |
T2487 |
dobj |
presence,confirmed |
R959 |
T2490 |
T2489 |
prep |
of,presence |
R960 |
T2491 |
T2492 |
det |
the,protein |
R961 |
T2492 |
T2490 |
pobj |
protein,of |
R962 |
T2493 |
T2489 |
prep |
during,presence |
R963 |
T2494 |
T2495 |
amod |
early,development |
R964 |
T2495 |
T2493 |
pobj |
development,during |
R965 |
T2496 |
T2487 |
punct |
.,confirmed |
R966 |
T2498 |
T2499 |
compound |
Annexin,A7 |
R967 |
T2499 |
T2500 |
compound |
A7,immunoreactivity |
R968 |
T2500 |
T2501 |
nsubjpass |
immunoreactivity,observed |
R969 |
T2502 |
T2500 |
acl |
using,immunoreactivity |
R970 |
T2503 |
T2504 |
nmod |
mAb,203 |
R971 |
T2504 |
T2502 |
dobj |
203,using |
R972 |
T2505 |
T2506 |
punct |
–,217 |
R973 |
T2506 |
T2504 |
prep |
217,203 |
R974 |
T2507 |
T2504 |
punct |
", ",203 |
R975 |
T2508 |
T2509 |
dep |
which,recognizes |
R976 |
T2509 |
T2504 |
relcl |
recognizes,203 |
R977 |
T2510 |
T2509 |
advmod |
specifically,recognizes |
R978 |
T2511 |
T2512 |
det |
both,isoforms |
R979 |
T2512 |
T2509 |
dobj |
isoforms,recognizes |
R980 |
T2513 |
T2512 |
prep |
of,isoforms |
R981 |
T2514 |
T2515 |
compound |
Annexin,A7 |
R982 |
T2515 |
T2513 |
pobj |
A7,of |
R983 |
T2516 |
T2501 |
punct |
", ",observed |
R984 |
T2517 |
T2501 |
aux |
can,observed |
R985 |
T2518 |
T2501 |
auxpass |
be,observed |
R986 |
T2519 |
T2501 |
prep |
in,observed |
R987 |
T2520 |
T2521 |
det |
the,types |
R988 |
T2521 |
T2519 |
pobj |
types,in |
R989 |
T2522 |
T2521 |
nummod |
two,types |
R990 |
T2523 |
T2521 |
compound |
cell,types |
R991 |
T2524 |
T2521 |
prep |
of,types |
R1509 |
T3749 |
T3750 |
dep |
which,exhibits |
R1510 |
T3750 |
T3738 |
relcl |
exhibits,cell |
R1511 |
T3751 |
T3750 |
advmod |
additionally,exhibits |
R1512 |
T3752 |
T3753 |
det |
an,signal |
R1513 |
T3753 |
T3750 |
dobj |
signal,exhibits |
R1514 |
T3754 |
T3753 |
amod |
intense,signal |
R1515 |
T3755 |
T3750 |
prep |
in,exhibits |
R1516 |
T3756 |
T3757 |
det |
the,cytoplasm |
R1517 |
T3757 |
T3755 |
pobj |
cytoplasm,in |
R1518 |
T3758 |
T3755 |
cc |
and,in |
R1519 |
T3759 |
T3755 |
conj |
in,in |
R1520 |
T3760 |
T3761 |
amod |
cellular,branches |
R1521 |
T3761 |
T3759 |
pobj |
branches,in |
R1522 |
T3762 |
T3715 |
punct |
.,demonstrates |
R1523 |
T3764 |
T3765 |
det |
A,type |
R1524 |
T3765 |
T3768 |
nsubj |
type,showed |
R1525 |
T3766 |
T3765 |
amod |
further,type |
R1526 |
T3767 |
T3765 |
compound |
cell,type |
R1527 |
T3769 |
T3765 |
prep |
of,type |
R1528 |
T3770 |
T3771 |
det |
the,isocortex |
R1570 |
T3816 |
T3817 |
prep |
In,detected |
R1571 |
T3818 |
T3819 |
det |
the,formation |
R1572 |
T3819 |
T3816 |
pobj |
formation,In |
R1573 |
T3820 |
T3819 |
amod |
hippocampal,formation |
R3958 |
T7901 |
T7902 |
punct |
[,34 |
R3959 |
T7902 |
T7863 |
parataxis |
34,suggested |
R3961 |
T7903 |
T7902 |
punct |
],34 |
R3962 |
T7904 |
T7863 |
punct |
.,suggested |
R3964 |
T7906 |
T7907 |
det |
The,annexins |
R3965 |
T7907 |
T7908 |
nsubj |
annexins,participate |