PMC:1084331 / 8301-8625 JSONTXT

{"project":"2_test","denotations":[{"id":"15836427-10850491-84719630","span":{"begin":320,"end":322},"obj":"10850491"},{"id":"T94600","span":{"begin":320,"end":322},"obj":"10850491"}],"text":"(B) PCR and Southern blot analysis of Er81EWS-Pea3 wild-type (+/+), heterozygous (+/−), and homozygous (−/−) genomic DNA to detect the mutant allele. PCR primer pairs (EWS-Pea3ki) were used to detect specifically the recombined allele, and a primer pair in exon2 was used to detect the presence of the wild-type allele [14]."}