Id |
Subject |
Object |
Predicate |
Lexical cue |
T3609 |
0-3 |
NN |
denotes |
EWS |
T3610 |
4-8 |
NN |
denotes |
Pea3 |
T3611 |
3-4 |
HYPH |
denotes |
- |
T3612 |
13-20 |
VB |
denotes |
Replace |
T3613 |
9-12 |
MD |
denotes |
Can |
T3614 |
21-25 |
NN |
denotes |
Er81 |
T3615 |
26-34 |
NN |
denotes |
Function |
T3616 |
35-37 |
IN |
denotes |
in |
T3617 |
38-49 |
VBG |
denotes |
Controlling |
T3618 |
50-52 |
NN |
denotes |
Ia |
T3619 |
62-73 |
NNS |
denotes |
Projections |
T3620 |
53-61 |
JJ |
denotes |
Afferent |
T3621 |
73-250 |
sentence |
denotes |
We first defined an ETS transcription regulator that is able to replace the function of Er81 within proprioceptive afferents to direct projections into the ventral spinal cord. |
T3622 |
74-76 |
PRP |
denotes |
We |
T3623 |
83-90 |
VBD |
denotes |
defined |
T3624 |
77-82 |
RB |
denotes |
first |
T3625 |
91-93 |
DT |
denotes |
an |
T3626 |
112-121 |
NN |
denotes |
regulator |
T3627 |
94-97 |
NN |
denotes |
ETS |
T3628 |
98-111 |
NN |
denotes |
transcription |
T3629 |
122-126 |
WDT |
denotes |
that |
T3630 |
127-129 |
VBZ |
denotes |
is |
T3631 |
130-134 |
JJ |
denotes |
able |
T3632 |
135-137 |
TO |
denotes |
to |
T3633 |
138-145 |
VB |
denotes |
replace |
T3634 |
146-149 |
DT |
denotes |
the |
T3635 |
150-158 |
NN |
denotes |
function |
T3636 |
159-161 |
IN |
denotes |
of |
T3637 |
162-166 |
NN |
denotes |
Er81 |
T3638 |
167-173 |
IN |
denotes |
within |
T3639 |
174-188 |
JJ |
denotes |
proprioceptive |
T3640 |
189-198 |
NNS |
denotes |
afferents |
T3641 |
199-201 |
TO |
denotes |
to |
T3642 |
202-208 |
VB |
denotes |
direct |
T3643 |
209-220 |
NNS |
denotes |
projections |
T3644 |
221-225 |
IN |
denotes |
into |
T3645 |
226-229 |
DT |
denotes |
the |
T3646 |
245-249 |
NN |
denotes |
cord |
T3647 |
230-237 |
JJ |
denotes |
ventral |
T3648 |
238-244 |
JJ |
denotes |
spinal |
T3649 |
249-250 |
. |
denotes |
. |
T3650 |
250-431 |
sentence |
denotes |
Er81, Pea3, and Erm constitute the Pea3 subfamily of ETS transcription factors, show a high degree of amino acid identity, and bind to very similar DNA target sequences [17,18,19]. |
T3651 |
251-255 |
NN |
denotes |
Er81 |
T3652 |
271-281 |
VBP |
denotes |
constitute |
T3653 |
255-257 |
, |
denotes |
, |
T3654 |
257-261 |
NN |
denotes |
Pea3 |
T3655 |
261-263 |
, |
denotes |
, |
T3656 |
263-266 |
CC |
denotes |
and |
T3657 |
267-270 |
NN |
denotes |
Erm |
T3658 |
282-285 |
DT |
denotes |
the |
T3659 |
291-300 |
NN |
denotes |
subfamily |
T3660 |
286-290 |
NN |
denotes |
Pea3 |
T3661 |
301-303 |
IN |
denotes |
of |
T3662 |
304-307 |
NN |
denotes |
ETS |
T3663 |
322-329 |
NNS |
denotes |
factors |
T3664 |
308-321 |
NN |
denotes |
transcription |
T3665 |
329-331 |
, |
denotes |
, |
T3666 |
331-335 |
VBP |
denotes |
show |
T3667 |
336-337 |
DT |
denotes |
a |
T3668 |
343-349 |
NN |
denotes |
degree |
T3669 |
338-342 |
JJ |
denotes |
high |
T3670 |
350-352 |
IN |
denotes |
of |
T3671 |
353-358 |
NN |
denotes |
amino |
T3672 |
359-363 |
NN |
denotes |
acid |
T3673 |
364-372 |
NN |
denotes |
identity |
T3674 |
372-374 |
, |
denotes |
, |
T3675 |
374-377 |
CC |
denotes |
and |
T3676 |
378-382 |
VBP |
denotes |
bind |
T3677 |
383-385 |
IN |
denotes |
to |
T3678 |
386-390 |
RB |
denotes |
very |
T3679 |
391-398 |
JJ |
denotes |
similar |
T3680 |
410-419 |
NNS |
denotes |
sequences |
T3681 |
399-402 |
NN |
denotes |
DNA |
T3682 |
403-409 |
NN |
denotes |
target |
T3683 |
420-421 |
-LRB- |
denotes |
[ |
T3684 |
427-429 |
CD |
denotes |
19 |
T3685 |
421-423 |
CD |
denotes |
17 |
T3686 |
423-424 |
, |
denotes |
, |
T3687 |
424-426 |
CD |
denotes |
18 |
T3688 |
426-427 |
, |
denotes |
, |
T3689 |
429-430 |
-RRB- |
denotes |
] |
T3690 |
430-431 |
. |
denotes |
. |
T3691 |
431-705 |
sentence |
denotes |
Nevertheless, when introduced into the Er81 locus in analogy to a previously used targeting strategy (data not shown; [14]), neither Pea3 nor Erm could rescue Ia proprioceptive afferent projections to extensively invade the ventral horn of the spinal cord (data not shown). |
T3692 |
432-444 |
RB |
denotes |
Nevertheless |
T3693 |
584-590 |
VB |
denotes |
rescue |
T3694 |
444-446 |
, |
denotes |
, |
T3695 |
446-450 |
WRB |
denotes |
when |
T3696 |
451-461 |
VBN |
denotes |
introduced |
T3697 |
462-466 |
IN |
denotes |
into |
T3698 |
467-470 |
DT |
denotes |
the |
T3699 |
476-481 |
NN |
denotes |
locus |
T3700 |
471-475 |
NN |
denotes |
Er81 |
T3701 |
482-484 |
IN |
denotes |
in |
T3702 |
485-492 |
NN |
denotes |
analogy |
T3703 |
493-495 |
IN |
denotes |
to |
T3704 |
496-497 |
DT |
denotes |
a |
T3705 |
524-532 |
NN |
denotes |
strategy |
T3706 |
498-508 |
RB |
denotes |
previously |
T3707 |
509-513 |
VBN |
denotes |
used |
T3708 |
514-523 |
NN |
denotes |
targeting |
T3709 |
533-534 |
-LRB- |
denotes |
( |
T3710 |
543-548 |
VBN |
denotes |
shown |
T3711 |
534-538 |
NNS |
denotes |
data |
T3712 |
539-542 |
RB |
denotes |
not |
T3713 |
548-549 |
: |
denotes |
; |
T3714 |
550-551 |
-LRB- |
denotes |
[ |
T3715 |
551-553 |
CD |
denotes |
14 |
T3716 |
553-554 |
-RRB- |
denotes |
] |
T3717 |
554-555 |
-RRB- |
denotes |
) |
T3718 |
555-557 |
, |
denotes |
, |
T3719 |
557-564 |
CC |
denotes |
neither |
T3720 |
565-569 |
NN |
denotes |
Pea3 |
T3721 |
570-573 |
CC |
denotes |
nor |
T3722 |
574-577 |
NN |
denotes |
Erm |
T3723 |
578-583 |
MD |
denotes |
could |
T3724 |
591-593 |
NN |
denotes |
Ia |
T3725 |
594-608 |
JJ |
denotes |
proprioceptive |
T3726 |
618-629 |
NNS |
denotes |
projections |
T3727 |
609-617 |
JJ |
denotes |
afferent |
T3728 |
630-632 |
TO |
denotes |
to |
T3729 |
645-651 |
VB |
denotes |
invade |
T3730 |
633-644 |
RB |
denotes |
extensively |
T3731 |
652-655 |
DT |
denotes |
the |
T3732 |
664-668 |
NN |
denotes |
horn |
T3733 |
656-663 |
JJ |
denotes |
ventral |
T3734 |
669-671 |
IN |
denotes |
of |
T3735 |
672-675 |
DT |
denotes |
the |
T3736 |
683-687 |
NN |
denotes |
cord |
T3737 |
676-682 |
JJ |
denotes |
spinal |
T3738 |
688-689 |
-LRB- |
denotes |
( |
T3739 |
698-703 |
VBN |
denotes |
shown |
T3740 |
689-693 |
NNS |
denotes |
data |
T3741 |
694-697 |
RB |
denotes |
not |
T3742 |
703-704 |
-RRB- |
denotes |
) |
T3743 |
704-705 |
. |
denotes |
. |
T3744 |
705-949 |
sentence |
denotes |
These findings prompted us to analyze mice in which we integrated EWS-Pea3, a break-point fusion product between the amino-terminal domain of the Ewing sarcoma (EWS) gene and the Pea3 DNA binding domain [20,21], into the Er81 locus (Figure 1). |
T3745 |
706-711 |
DT |
denotes |
These |
T3746 |
712-720 |
NNS |
denotes |
findings |
T3747 |
721-729 |
VBD |
denotes |
prompted |
T3748 |
730-732 |
PRP |
denotes |
us |
T3749 |
733-735 |
TO |
denotes |
to |
T3750 |
736-743 |
VB |
denotes |
analyze |
T3751 |
744-748 |
NNS |
denotes |
mice |
T3752 |
749-751 |
IN |
denotes |
in |
T3753 |
761-771 |
VBD |
denotes |
integrated |
T3754 |
752-757 |
WDT |
denotes |
which |
T3755 |
758-760 |
PRP |
denotes |
we |
T3756 |
772-775 |
NN |
denotes |
EWS |
T3757 |
776-780 |
NN |
denotes |
Pea3 |
T3758 |
775-776 |
HYPH |
denotes |
- |
T3759 |
780-782 |
, |
denotes |
, |
T3760 |
782-783 |
DT |
denotes |
a |
T3761 |
803-810 |
NN |
denotes |
product |
T3762 |
784-789 |
NN |
denotes |
break |
T3763 |
790-795 |
NN |
denotes |
point |
T3764 |
789-790 |
HYPH |
denotes |
- |
T3765 |
796-802 |
NN |
denotes |
fusion |
T3766 |
811-818 |
IN |
denotes |
between |
T3767 |
819-822 |
DT |
denotes |
the |
T3768 |
838-844 |
NN |
denotes |
domain |
T3769 |
823-828 |
NN |
denotes |
amino |
T3770 |
829-837 |
JJ |
denotes |
terminal |
T3771 |
828-829 |
HYPH |
denotes |
- |
T3772 |
845-847 |
IN |
denotes |
of |
T3773 |
848-851 |
DT |
denotes |
the |
T3774 |
872-876 |
NN |
denotes |
gene |
T3775 |
852-857 |
NN |
denotes |
Ewing |
T3776 |
858-865 |
NN |
denotes |
sarcoma |
T3777 |
866-867 |
-LRB- |
denotes |
( |
T3778 |
867-870 |
NN |
denotes |
EWS |
T3779 |
870-871 |
-RRB- |
denotes |
) |
T3780 |
877-880 |
CC |
denotes |
and |
T3781 |
881-884 |
DT |
denotes |
the |
T3782 |
902-908 |
NN |
denotes |
domain |
T3783 |
885-889 |
NN |
denotes |
Pea3 |
T3784 |
890-893 |
NN |
denotes |
DNA |
T3785 |
894-901 |
VBG |
denotes |
binding |
T3786 |
909-910 |
-LRB- |
denotes |
[ |
T3787 |
913-915 |
CD |
denotes |
21 |
T3788 |
910-912 |
CD |
denotes |
20 |
T3789 |
912-913 |
, |
denotes |
, |
T3790 |
915-916 |
-RRB- |
denotes |
] |
T3791 |
916-918 |
, |
denotes |
, |
T3792 |
918-922 |
IN |
denotes |
into |
T3793 |
923-926 |
DT |
denotes |
the |
T3794 |
932-937 |
NN |
denotes |
locus |
T3795 |
927-931 |
NN |
denotes |
Er81 |
T3796 |
938-939 |
-LRB- |
denotes |
( |
T3797 |
939-945 |
NN |
denotes |
Figure |
T3798 |
946-947 |
CD |
denotes |
1 |
T3799 |
947-948 |
-RRB- |
denotes |
) |
T3800 |
948-949 |
. |
denotes |
. |
T3801 |
949-1169 |
sentence |
denotes |
We found that in a luciferase-enzyme-based cell culture transfection assay, EWS-Pea3 showed stronger transactivation activity than Er81 or Pea3 (Figure 1J; data not shown), in agreement with previous studies [22,23,24]. |
T3802 |
950-952 |
PRP |
denotes |
We |
T3803 |
953-958 |
VBD |
denotes |
found |
T3804 |
959-963 |
IN |
denotes |
that |
T3805 |
1035-1041 |
VBD |
denotes |
showed |
T3806 |
964-966 |
IN |
denotes |
in |
T3807 |
967-968 |
DT |
denotes |
a |
T3808 |
1019-1024 |
NN |
denotes |
assay |
T3809 |
969-979 |
NN |
denotes |
luciferase |
T3810 |
980-986 |
NN |
denotes |
enzyme |
T3811 |
979-980 |
HYPH |
denotes |
- |
T3812 |
987-992 |
VBN |
denotes |
based |
T3813 |
986-987 |
HYPH |
denotes |
- |
T3814 |
993-997 |
NN |
denotes |
cell |
T3815 |
998-1005 |
NN |
denotes |
culture |
T3816 |
1006-1018 |
NN |
denotes |
transfection |
T3817 |
1024-1026 |
, |
denotes |
, |
T3818 |
1026-1029 |
NN |
denotes |
EWS |
T3819 |
1030-1034 |
NN |
denotes |
Pea3 |
T3820 |
1029-1030 |
HYPH |
denotes |
- |
T3821 |
1042-1050 |
JJR |
denotes |
stronger |
T3822 |
1067-1075 |
NN |
denotes |
activity |
T3823 |
1051-1066 |
NN |
denotes |
transactivation |
T3824 |
1076-1080 |
IN |
denotes |
than |
T3825 |
1081-1085 |
NN |
denotes |
Er81 |
T3826 |
1086-1088 |
CC |
denotes |
or |
T3827 |
1089-1093 |
NN |
denotes |
Pea3 |
T3828 |
1094-1095 |
-LRB- |
denotes |
( |
T3829 |
1102-1104 |
NN |
denotes |
1J |
T3830 |
1095-1101 |
NN |
denotes |
Figure |
T3831 |
1104-1105 |
: |
denotes |
; |
T3832 |
1106-1110 |
NNS |
denotes |
data |
T3833 |
1115-1120 |
VBN |
denotes |
shown |
T3834 |
1111-1114 |
RB |
denotes |
not |
T3835 |
1120-1121 |
-RRB- |
denotes |
) |
T3836 |
1121-1123 |
, |
denotes |
, |
T3837 |
1123-1125 |
IN |
denotes |
in |
T3838 |
1126-1135 |
NN |
denotes |
agreement |
T3839 |
1136-1140 |
IN |
denotes |
with |
T3840 |
1141-1149 |
JJ |
denotes |
previous |
T3841 |
1150-1157 |
NNS |
denotes |
studies |
T3842 |
1158-1159 |
-LRB- |
denotes |
[ |
T3843 |
1165-1167 |
CD |
denotes |
24 |
T3844 |
1159-1161 |
CD |
denotes |
22 |
T3845 |
1161-1162 |
, |
denotes |
, |
T3846 |
1162-1164 |
CD |
denotes |
23 |
T3847 |
1164-1165 |
, |
denotes |
, |
T3848 |
1167-1168 |
-RRB- |
denotes |
] |
T3849 |
1168-1169 |
. |
denotes |
. |
T3850 |
1169-1339 |
sentence |
denotes |
Moreover, transactivation by EWS-Pea3 was abolished by mutation of ETS-binding sites in the reporter plasmid, demonstrating ETS-binding-site dependence (data not shown). |
T3851 |
1170-1178 |
RB |
denotes |
Moreover |
T3852 |
1212-1221 |
VBN |
denotes |
abolished |
T3853 |
1178-1180 |
, |
denotes |
, |
T3854 |
1180-1195 |
NN |
denotes |
transactivation |
T3855 |
1196-1198 |
IN |
denotes |
by |
T3856 |
1199-1202 |
NN |
denotes |
EWS |
T3857 |
1203-1207 |
NN |
denotes |
Pea3 |
T3858 |
1202-1203 |
HYPH |
denotes |
- |
T3859 |
1208-1211 |
VBD |
denotes |
was |
T3860 |
1222-1224 |
IN |
denotes |
by |
T3861 |
1225-1233 |
NN |
denotes |
mutation |
T3862 |
1234-1236 |
IN |
denotes |
of |
T3863 |
1237-1240 |
NN |
denotes |
ETS |
T3864 |
1241-1248 |
VBG |
denotes |
binding |
T3865 |
1240-1241 |
HYPH |
denotes |
- |
T3866 |
1249-1254 |
NNS |
denotes |
sites |
T3867 |
1255-1257 |
IN |
denotes |
in |
T3868 |
1258-1261 |
DT |
denotes |
the |
T3869 |
1271-1278 |
NN |
denotes |
plasmid |
T3870 |
1262-1270 |
NN |
denotes |
reporter |
T3871 |
1278-1280 |
, |
denotes |
, |
T3872 |
1280-1293 |
VBG |
denotes |
demonstrating |
T3873 |
1294-1297 |
NN |
denotes |
ETS |
T3874 |
1298-1305 |
VBG |
denotes |
binding |
T3875 |
1297-1298 |
HYPH |
denotes |
- |
T3876 |
1306-1310 |
NN |
denotes |
site |
T3877 |
1305-1306 |
HYPH |
denotes |
- |
T3878 |
1311-1321 |
NN |
denotes |
dependence |
T3879 |
1322-1323 |
-LRB- |
denotes |
( |
T3880 |
1332-1337 |
VBN |
denotes |
shown |
T3881 |
1323-1327 |
NNS |
denotes |
data |
T3882 |
1328-1331 |
RB |
denotes |
not |
T3883 |
1337-1338 |
-RRB- |
denotes |
) |
T3884 |
1338-1339 |
. |
denotes |
. |
T3885 |
1339-3331 |
sentence |
denotes |
Figure 1 Replacement of Er81 by EWS-Pea3
(A) Generation of Er81EWS-Pea3 mutant mice. Above is the organization of the Er81 genomic locus in the region targeted by homologous recombination in analogy to [14]. Exons 1–4 are shown as light blue boxes, and the Er81 start codon in exon 2 is indicated as ATG. The probe used to detect homologous recombination is shown as a grey box. Below is replacement of Er81 by EWS-Pea3 through the integration of EWS-Pea3 in frame with the endogenous start codon of the Er81 locus in exon 2 (in analogy to [14]).
(B) PCR and Southern blot analysis of Er81EWS-Pea3 wild-type (+/+), heterozygous (+/−), and homozygous (−/−) genomic DNA to detect the mutant allele. PCR primer pairs (EWS-Pea3ki) were used to detect specifically the recombined allele, and a primer pair in exon2 was used to detect the presence of the wild-type allele [14].
(C–E) Analysis of Er81 expression in lumbar DRG neurons of E16.5 wild-type (C), Er81−/− (D), and Er81EWS-Pea3/− (E) embryos. Inset in lower right corner of each panel shows Isl1 expression in the respective DRG.
(F–H) PV expression in lumbar DRG of E16.5 wild-type (F), Er81−/− (G), and Er81EWS-Pea3/− (H) embryos. Confocal scans were performed with equal gain intensity.
(J) Transcriptional transactivation of luciferase expression from a minimal reporter construct containing five consensus ETS DNA-binding sites ( GCCGGAAGC; [18,19]) and a minimal TK promoter upon transient transfection of Er81 (n ≥ 7; 3.03 ± 0.66) or EWS-Pea3 (n ≥ 7; 20.3 ± 2.7). Relative luciferase activity normalized to control (Con).
Scale bar: 80 μm. Expression of Er81 in DRG neurons of embryos containing integration of EWS-Pea3 in the Er81 locus (Er81EWS-Pea3/−) was abolished (Figure 1E), and the expression level of the calcium-binding protein Parvalbumin (PV) in proprioceptive afferents, which is decreased approximately 5- to 10-fold in Er81 mutants [14], was comparable to wild-type levels in Er81EWS-Pea3/− embryos (Figure 1F–1H). |
T3886 |
2942-2952 |
NN |
denotes |
Expression |
T3887 |
3061-3070 |
VBN |
denotes |
abolished |
T3888 |
2953-2955 |
IN |
denotes |
of |
T3889 |
2956-2960 |
NN |
denotes |
Er81 |
T3890 |
2961-2963 |
IN |
denotes |
in |
T3891 |
2964-2967 |
NN |
denotes |
DRG |
T3892 |
2968-2975 |
NNS |
denotes |
neurons |
T3893 |
2976-2978 |
IN |
denotes |
of |
T3894 |
2979-2986 |
NNS |
denotes |
embryos |
T3895 |
2987-2997 |
VBG |
denotes |
containing |
T3896 |
2998-3009 |
NN |
denotes |
integration |
T3897 |
3010-3012 |
IN |
denotes |
of |
T3898 |
3013-3016 |
NN |
denotes |
EWS |
T3899 |
3017-3021 |
NN |
denotes |
Pea3 |
T3900 |
3016-3017 |
HYPH |
denotes |
- |
T3901 |
3022-3024 |
IN |
denotes |
in |
T3902 |
3025-3028 |
DT |
denotes |
the |
T3903 |
3034-3039 |
NN |
denotes |
locus |
T3904 |
3029-3033 |
NN |
denotes |
Er81 |
T3905 |
3040-3041 |
-LRB- |
denotes |
( |
T3906 |
3041-3048 |
NN |
denotes |
Er81EWS |
T3907 |
3049-3053 |
NN |
denotes |
Pea3 |
T3908 |
3048-3049 |
HYPH |
denotes |
- |
T3909 |
3053-3054 |
HYPH |
denotes |
/ |
T3910 |
3054-3055 |
SYM |
denotes |
− |
T3911 |
3055-3056 |
-RRB- |
denotes |
) |
T3912 |
3057-3060 |
VBD |
denotes |
was |
T3913 |
3071-3072 |
-LRB- |
denotes |
( |
T3914 |
3079-3081 |
NN |
denotes |
1E |
T3915 |
3072-3078 |
NN |
denotes |
Figure |
T3916 |
3081-3082 |
-RRB- |
denotes |
) |
T3917 |
3082-3084 |
, |
denotes |
, |
T3918 |
3084-3087 |
CC |
denotes |
and |
T3919 |
3088-3091 |
DT |
denotes |
the |
T3920 |
3103-3108 |
NN |
denotes |
level |
T3921 |
3092-3102 |
NN |
denotes |
expression |
T3922 |
3255-3258 |
VBD |
denotes |
was |
T3923 |
3109-3111 |
IN |
denotes |
of |
T3924 |
3112-3115 |
DT |
denotes |
the |
T3925 |
3132-3139 |
NN |
denotes |
protein |
T3926 |
3116-3123 |
NN |
denotes |
calcium |
T3927 |
3124-3131 |
VBG |
denotes |
binding |
T3928 |
3123-3124 |
HYPH |
denotes |
- |
T3929 |
3140-3151 |
NN |
denotes |
Parvalbumin |
T3930 |
3152-3153 |
-LRB- |
denotes |
( |
T3931 |
3153-3155 |
NN |
denotes |
PV |
T3932 |
3155-3156 |
-RRB- |
denotes |
) |
T3933 |
3157-3159 |
IN |
denotes |
in |
T3934 |
3160-3174 |
JJ |
denotes |
proprioceptive |
T3935 |
3175-3184 |
NNS |
denotes |
afferents |
T3936 |
3184-3186 |
, |
denotes |
, |
T3937 |
3186-3191 |
WDT |
denotes |
which |
T3938 |
3195-3204 |
VBN |
denotes |
decreased |
T3939 |
3192-3194 |
VBZ |
denotes |
is |
T3940 |
3205-3218 |
RB |
denotes |
approximately |
T3941 |
3225-3227 |
CD |
denotes |
10 |
T3942 |
3219-3220 |
CD |
denotes |
5 |
T3943 |
3220-3221 |
HYPH |
denotes |
- |
T3944 |
3222-3224 |
IN |
denotes |
to |
T3945 |
3228-3232 |
RB |
denotes |
fold |
T3946 |
3227-3228 |
HYPH |
denotes |
- |
T3947 |
3233-3235 |
IN |
denotes |
in |
T3948 |
3236-3240 |
NN |
denotes |
Er81 |
T3949 |
3241-3248 |
NNS |
denotes |
mutants |
T3950 |
3249-3250 |
-LRB- |
denotes |
[ |
T3951 |
3250-3252 |
CD |
denotes |
14 |
T3952 |
3252-3253 |
-RRB- |
denotes |
] |
T3953 |
3253-3255 |
, |
denotes |
, |
T3954 |
3259-3269 |
JJ |
denotes |
comparable |
T3955 |
3270-3272 |
IN |
denotes |
to |
T3956 |
3273-3277 |
JJ |
denotes |
wild |
T3957 |
3278-3282 |
NN |
denotes |
type |
T3958 |
3277-3278 |
HYPH |
denotes |
- |
T3959 |
3283-3289 |
NNS |
denotes |
levels |
T3960 |
3290-3292 |
IN |
denotes |
in |
T3961 |
3293-3300 |
NN |
denotes |
Er81EWS |
T3962 |
3301-3305 |
NN |
denotes |
Pea3 |
T3963 |
3300-3301 |
HYPH |
denotes |
- |
T3964 |
3308-3315 |
NNS |
denotes |
embryos |
T3965 |
3305-3306 |
HYPH |
denotes |
/ |
T3966 |
3306-3307 |
SYM |
denotes |
− |
T3967 |
3316-3317 |
-LRB- |
denotes |
( |
T3968 |
3324-3326 |
NN |
denotes |
1F |
T3969 |
3317-3323 |
NN |
denotes |
Figure |
T3970 |
3326-3327 |
SYM |
denotes |
– |
T3971 |
3327-3329 |
NN |
denotes |
1H |
T3972 |
3329-3330 |
-RRB- |
denotes |
) |
T3973 |
3330-3331 |
. |
denotes |
. |
T3974 |
3331-3595 |
sentence |
denotes |
To further define DRG neuron differentiation in the presence of EWS-Pea3 in proprioceptive afferents in vivo, we assessed whether replacement of Er81 by EWS-Pea3 had an influence on neuronal survival or on the expression of proprioceptive-afferent-specific genes. |
T3975 |
3332-3334 |
TO |
denotes |
To |
T3976 |
3343-3349 |
VB |
denotes |
define |
T3977 |
3335-3342 |
RB |
denotes |
further |
T3978 |
3445-3453 |
VBD |
denotes |
assessed |
T3979 |
3350-3353 |
NN |
denotes |
DRG |
T3980 |
3354-3360 |
NN |
denotes |
neuron |
T3981 |
3361-3376 |
NN |
denotes |
differentiation |
T3982 |
3377-3379 |
IN |
denotes |
in |
T3983 |
3380-3383 |
DT |
denotes |
the |
T3984 |
3384-3392 |
NN |
denotes |
presence |
T3985 |
3393-3395 |
IN |
denotes |
of |
T3986 |
3396-3399 |
NN |
denotes |
EWS |
T3987 |
3400-3404 |
NN |
denotes |
Pea3 |
T3988 |
3399-3400 |
HYPH |
denotes |
- |
T3989 |
3405-3407 |
IN |
denotes |
in |
T3990 |
3408-3422 |
JJ |
denotes |
proprioceptive |
T3991 |
3423-3432 |
NNS |
denotes |
afferents |
T3992 |
3433-3435 |
FW |
denotes |
in |
T3993 |
3436-3440 |
FW |
denotes |
vivo |
T3994 |
3440-3442 |
, |
denotes |
, |
T3995 |
3442-3444 |
PRP |
denotes |
we |
T3996 |
3454-3461 |
IN |
denotes |
whether |
T3997 |
3494-3497 |
VBD |
denotes |
had |
T3998 |
3462-3473 |
NN |
denotes |
replacement |
T3999 |
3474-3476 |
IN |
denotes |
of |
T4000 |
3477-3481 |
NN |
denotes |
Er81 |
T4001 |
3482-3484 |
IN |
denotes |
by |
T4002 |
3485-3488 |
NN |
denotes |
EWS |
T4003 |
3489-3493 |
NN |
denotes |
Pea3 |
T4004 |
3488-3489 |
HYPH |
denotes |
- |
T4005 |
3498-3500 |
DT |
denotes |
an |
T4006 |
3501-3510 |
NN |
denotes |
influence |
T4007 |
3511-3513 |
IN |
denotes |
on |
T4008 |
3514-3522 |
JJ |
denotes |
neuronal |
T4009 |
3523-3531 |
NN |
denotes |
survival |
T4010 |
3532-3534 |
CC |
denotes |
or |
T4011 |
3535-3537 |
IN |
denotes |
on |
T4012 |
3538-3541 |
DT |
denotes |
the |
T4013 |
3542-3552 |
NN |
denotes |
expression |
T4014 |
3553-3555 |
IN |
denotes |
of |
T4015 |
3556-3570 |
JJ |
denotes |
proprioceptive |
T4016 |
3571-3579 |
JJ |
denotes |
afferent |
T4017 |
3570-3571 |
HYPH |
denotes |
- |
T4018 |
3580-3588 |
JJ |
denotes |
specific |
T4019 |
3579-3580 |
HYPH |
denotes |
- |
T4020 |
3589-3594 |
NNS |
denotes |
genes |
T4021 |
3594-3595 |
. |
denotes |
. |
T4022 |
3595-3922 |
sentence |
denotes |
Er81EWS-Pea3/− mice did not differ from wild-type in the number of proprioceptive afferent cell bodies within the DRG of L1 to L5, the expression of several genes normally expressed by proprioceptive afferents, and the lack of expression of genes not normally expressed in proprioceptive afferents (Figure S1; data not shown). |
T4023 |
3596-3603 |
NN |
denotes |
Er81EWS |
T4024 |
3604-3608 |
NN |
denotes |
Pea3 |
T4025 |
3603-3604 |
HYPH |
denotes |
- |
T4026 |
3611-3615 |
NNS |
denotes |
mice |
T4027 |
3608-3609 |
HYPH |
denotes |
/ |
T4028 |
3609-3610 |
SYM |
denotes |
− |
T4029 |
3624-3630 |
VB |
denotes |
differ |
T4030 |
3616-3619 |
VBD |
denotes |
did |
T4031 |
3620-3623 |
RB |
denotes |
not |
T4032 |
3631-3635 |
IN |
denotes |
from |
T4033 |
3636-3640 |
JJ |
denotes |
wild |
T4034 |
3641-3645 |
NN |
denotes |
type |
T4035 |
3640-3641 |
HYPH |
denotes |
- |
T4036 |
3646-3648 |
IN |
denotes |
in |
T4037 |
3649-3652 |
DT |
denotes |
the |
T4038 |
3653-3659 |
NN |
denotes |
number |
T4039 |
3660-3662 |
IN |
denotes |
of |
T4040 |
3663-3677 |
JJ |
denotes |
proprioceptive |
T4041 |
3692-3698 |
NNS |
denotes |
bodies |
T4042 |
3678-3686 |
JJ |
denotes |
afferent |
T4043 |
3687-3691 |
NN |
denotes |
cell |
T4044 |
3699-3705 |
IN |
denotes |
within |
T4045 |
3706-3709 |
DT |
denotes |
the |
T4046 |
3710-3713 |
NN |
denotes |
DRG |
T4047 |
3714-3716 |
IN |
denotes |
of |
T4048 |
3717-3719 |
NN |
denotes |
L1 |
T4049 |
3720-3722 |
IN |
denotes |
to |
T4050 |
3723-3725 |
NN |
denotes |
L5 |
T4051 |
3725-3727 |
, |
denotes |
, |
T4052 |
3727-3730 |
DT |
denotes |
the |
T4053 |
3731-3741 |
NN |
denotes |
expression |
T4054 |
3742-3744 |
IN |
denotes |
of |
T4055 |
3745-3752 |
JJ |
denotes |
several |
T4056 |
3753-3758 |
NNS |
denotes |
genes |
T4057 |
3759-3767 |
RB |
denotes |
normally |
T4058 |
3768-3777 |
VBN |
denotes |
expressed |
T4059 |
3778-3780 |
IN |
denotes |
by |
T4060 |
3781-3795 |
JJ |
denotes |
proprioceptive |
T4061 |
3796-3805 |
NNS |
denotes |
afferents |
T4062 |
3805-3807 |
, |
denotes |
, |
T4063 |
3807-3810 |
CC |
denotes |
and |
T4064 |
3811-3814 |
DT |
denotes |
the |
T4065 |
3815-3819 |
NN |
denotes |
lack |
T4066 |
3820-3822 |
IN |
denotes |
of |
T4067 |
3823-3833 |
NN |
denotes |
expression |
T4068 |
3834-3836 |
IN |
denotes |
of |
T4069 |
3837-3842 |
NNS |
denotes |
genes |
T4070 |
3843-3846 |
RB |
denotes |
not |
T4071 |
3856-3865 |
VBN |
denotes |
expressed |
T4072 |
3847-3855 |
RB |
denotes |
normally |
T4073 |
3866-3868 |
IN |
denotes |
in |
T4074 |
3869-3883 |
JJ |
denotes |
proprioceptive |
T4075 |
3884-3893 |
NNS |
denotes |
afferents |
T4076 |
3894-3895 |
-LRB- |
denotes |
( |
T4077 |
3902-3904 |
NN |
denotes |
S1 |
T4078 |
3895-3901 |
NN |
denotes |
Figure |
T4079 |
3904-3905 |
: |
denotes |
; |
T4080 |
3906-3910 |
NNS |
denotes |
data |
T4081 |
3915-3920 |
VBN |
denotes |
shown |
T4082 |
3911-3914 |
RB |
denotes |
not |
T4083 |
3920-3921 |
-RRB- |
denotes |
) |
T4084 |
3921-3922 |
. |
denotes |
. |
T4085 |
3922-4138 |
sentence |
denotes |
Together, these findings suggest that the expression of EWS-Pea3 from the normal time of onset mimics the function of Er81 as assessed by induction and maintenance of gene expression within proprioceptive afferents. |
T4086 |
3923-3931 |
RB |
denotes |
Together |
T4087 |
3948-3955 |
VBP |
denotes |
suggest |
T4088 |
3931-3933 |
, |
denotes |
, |
T4089 |
3933-3938 |
DT |
denotes |
these |
T4090 |
3939-3947 |
NNS |
denotes |
findings |
T4091 |
3956-3960 |
IN |
denotes |
that |
T4092 |
4018-4024 |
VBZ |
denotes |
mimics |
T4093 |
3961-3964 |
DT |
denotes |
the |
T4094 |
3965-3975 |
NN |
denotes |
expression |
T4095 |
3976-3978 |
IN |
denotes |
of |
T4096 |
3979-3982 |
NN |
denotes |
EWS |
T4097 |
3983-3987 |
NN |
denotes |
Pea3 |
T4098 |
3982-3983 |
HYPH |
denotes |
- |
T4099 |
3988-3992 |
IN |
denotes |
from |
T4100 |
3993-3996 |
DT |
denotes |
the |
T4101 |
4004-4008 |
NN |
denotes |
time |
T4102 |
3997-4003 |
JJ |
denotes |
normal |
T4103 |
4009-4011 |
IN |
denotes |
of |
T4104 |
4012-4017 |
NN |
denotes |
onset |
T4105 |
4025-4028 |
DT |
denotes |
the |
T4106 |
4029-4037 |
NN |
denotes |
function |
T4107 |
4038-4040 |
IN |
denotes |
of |
T4108 |
4041-4045 |
NN |
denotes |
Er81 |
T4109 |
4046-4048 |
IN |
denotes |
as |
T4110 |
4049-4057 |
VBN |
denotes |
assessed |
T4111 |
4058-4060 |
IN |
denotes |
by |
T4112 |
4061-4070 |
NN |
denotes |
induction |
T4113 |
4071-4074 |
CC |
denotes |
and |
T4114 |
4075-4086 |
NN |
denotes |
maintenance |
T4115 |
4087-4089 |
IN |
denotes |
of |
T4116 |
4090-4094 |
NN |
denotes |
gene |
T4117 |
4095-4105 |
NN |
denotes |
expression |
T4118 |
4106-4112 |
IN |
denotes |
within |
T4119 |
4113-4127 |
JJ |
denotes |
proprioceptive |
T4120 |
4128-4137 |
NNS |
denotes |
afferents |
T4121 |
4137-4138 |
. |
denotes |
. |
T4122 |
4138-4383 |
sentence |
denotes |
To determine the extent of rescue of Ia proprioceptive afferent projections into the ventral spinal cord of Er81 mutant mice achieved by expression of EWS-Pea3, we traced intraspinal afferent projections by axonal labeling of PV (Figure 2A–2C). |
T4123 |
4139-4141 |
TO |
denotes |
To |
T4124 |
4142-4151 |
VB |
denotes |
determine |
T4125 |
4303-4309 |
VBD |
denotes |
traced |
T4126 |
4152-4155 |
DT |
denotes |
the |
T4127 |
4156-4162 |
NN |
denotes |
extent |
T4128 |
4163-4165 |
IN |
denotes |
of |
T4129 |
4166-4172 |
NN |
denotes |
rescue |
T4130 |
4173-4175 |
IN |
denotes |
of |
T4131 |
4176-4178 |
NN |
denotes |
Ia |
T4132 |
4203-4214 |
NNS |
denotes |
projections |
T4133 |
4179-4193 |
JJ |
denotes |
proprioceptive |
T4134 |
4194-4202 |
JJ |
denotes |
afferent |
T4135 |
4215-4219 |
IN |
denotes |
into |
T4136 |
4220-4223 |
DT |
denotes |
the |
T4137 |
4239-4243 |
NN |
denotes |
cord |
T4138 |
4224-4231 |
JJ |
denotes |
ventral |
T4139 |
4232-4238 |
JJ |
denotes |
spinal |
T4140 |
4244-4246 |
IN |
denotes |
of |
T4141 |
4247-4251 |
NN |
denotes |
Er81 |
T4142 |
4259-4263 |
NNS |
denotes |
mice |
T4143 |
4252-4258 |
NN |
denotes |
mutant |
T4144 |
4264-4272 |
VBN |
denotes |
achieved |
T4145 |
4273-4275 |
IN |
denotes |
by |
T4146 |
4276-4286 |
NN |
denotes |
expression |
T4147 |
4287-4289 |
IN |
denotes |
of |
T4148 |
4290-4293 |
NN |
denotes |
EWS |
T4149 |
4294-4298 |
NN |
denotes |
Pea3 |
T4150 |
4293-4294 |
HYPH |
denotes |
- |
T4151 |
4298-4300 |
, |
denotes |
, |
T4152 |
4300-4302 |
PRP |
denotes |
we |
T4153 |
4310-4321 |
JJ |
denotes |
intraspinal |
T4154 |
4331-4342 |
NNS |
denotes |
projections |
T4155 |
4322-4330 |
JJ |
denotes |
afferent |
T4156 |
4343-4345 |
IN |
denotes |
by |
T4157 |
4346-4352 |
JJ |
denotes |
axonal |
T4158 |
4353-4361 |
NN |
denotes |
labeling |
T4159 |
4362-4364 |
IN |
denotes |
of |
T4160 |
4365-4367 |
NN |
denotes |
PV |
T4161 |
4368-4369 |
-LRB- |
denotes |
( |
T4162 |
4376-4378 |
NN |
denotes |
2A |
T4163 |
4369-4375 |
NN |
denotes |
Figure |
T4164 |
4378-4379 |
SYM |
denotes |
– |
T4165 |
4379-4381 |
NN |
denotes |
2C |
T4166 |
4381-4382 |
-RRB- |
denotes |
) |
T4167 |
4382-4383 |
. |
denotes |
. |
T4168 |
4383-4605 |
sentence |
denotes |
In addition, to analyze axon ingrowth independent of the level of PV expression in DRG neurons, we used anterograde labeling of afferent fibers by applying fluorescently labeled dextran to cut dorsal roots (Figure 2D–2F). |
T4169 |
4384-4386 |
IN |
denotes |
In |
T4170 |
4483-4487 |
VBD |
denotes |
used |
T4171 |
4387-4395 |
NN |
denotes |
addition |
T4172 |
4395-4397 |
, |
denotes |
, |
T4173 |
4397-4399 |
TO |
denotes |
to |
T4174 |
4400-4407 |
VB |
denotes |
analyze |
T4175 |
4408-4412 |
NN |
denotes |
axon |
T4176 |
4413-4421 |
NN |
denotes |
ingrowth |
T4177 |
4422-4433 |
RB |
denotes |
independent |
T4178 |
4434-4436 |
IN |
denotes |
of |
T4179 |
4437-4440 |
DT |
denotes |
the |
T4180 |
4441-4446 |
NN |
denotes |
level |
T4181 |
4447-4449 |
IN |
denotes |
of |
T4182 |
4450-4452 |
NN |
denotes |
PV |
T4183 |
4453-4463 |
NN |
denotes |
expression |
T4184 |
4464-4466 |
IN |
denotes |
in |
T4185 |
4467-4470 |
NN |
denotes |
DRG |
T4186 |
4471-4478 |
NNS |
denotes |
neurons |
T4187 |
4478-4480 |
, |
denotes |
, |
T4188 |
4480-4482 |
PRP |
denotes |
we |
T4189 |
4488-4499 |
JJ |
denotes |
anterograde |
T4190 |
4500-4508 |
NN |
denotes |
labeling |
T4191 |
4509-4511 |
IN |
denotes |
of |
T4192 |
4512-4520 |
JJ |
denotes |
afferent |
T4193 |
4521-4527 |
NNS |
denotes |
fibers |
T4194 |
4528-4530 |
IN |
denotes |
by |
T4195 |
4531-4539 |
VBG |
denotes |
applying |
T4196 |
4540-4553 |
RB |
denotes |
fluorescently |
T4197 |
4554-4561 |
VBN |
denotes |
labeled |
T4198 |
4562-4569 |
NN |
denotes |
dextran |
T4199 |
4570-4572 |
IN |
denotes |
to |
T4200 |
4573-4576 |
VBN |
denotes |
cut |
T4201 |
4584-4589 |
NNS |
denotes |
roots |
T4202 |
4577-4583 |
JJ |
denotes |
dorsal |
T4203 |
4590-4591 |
-LRB- |
denotes |
( |
T4204 |
4598-4600 |
NN |
denotes |
2D |
T4205 |
4591-4597 |
NN |
denotes |
Figure |
T4206 |
4600-4601 |
SYM |
denotes |
– |
T4207 |
4601-4603 |
NN |
denotes |
2F |
T4208 |
4603-4604 |
-RRB- |
denotes |
) |
T4209 |
4604-4605 |
. |
denotes |
. |
T4210 |
4605-4753 |
sentence |
denotes |
Using both assays, we found extensive rescue of the projections into the ventral horn of the spinal cord in Er81EWS-Pea3/− mice (Figure 2C and 2F). |
T4211 |
4606-4611 |
VBG |
denotes |
Using |
T4212 |
4628-4633 |
VBD |
denotes |
found |
T4213 |
4612-4616 |
DT |
denotes |
both |
T4214 |
4617-4623 |
NNS |
denotes |
assays |
T4215 |
4623-4625 |
, |
denotes |
, |
T4216 |
4625-4627 |
PRP |
denotes |
we |
T4217 |
4634-4643 |
JJ |
denotes |
extensive |
T4218 |
4644-4650 |
NN |
denotes |
rescue |
T4219 |
4651-4653 |
IN |
denotes |
of |
T4220 |
4654-4657 |
DT |
denotes |
the |
T4221 |
4658-4669 |
NNS |
denotes |
projections |
T4222 |
4670-4674 |
IN |
denotes |
into |
T4223 |
4675-4678 |
DT |
denotes |
the |
T4224 |
4687-4691 |
NN |
denotes |
horn |
T4225 |
4679-4686 |
JJ |
denotes |
ventral |
T4226 |
4692-4694 |
IN |
denotes |
of |
T4227 |
4695-4698 |
DT |
denotes |
the |
T4228 |
4706-4710 |
NN |
denotes |
cord |
T4229 |
4699-4705 |
JJ |
denotes |
spinal |
T4230 |
4711-4713 |
IN |
denotes |
in |
T4231 |
4714-4721 |
NN |
denotes |
Er81EWS |
T4232 |
4722-4726 |
NN |
denotes |
Pea3 |
T4233 |
4721-4722 |
HYPH |
denotes |
- |
T4234 |
4729-4733 |
NNS |
denotes |
mice |
T4235 |
4726-4727 |
HYPH |
denotes |
/ |
T4236 |
4727-4728 |
SYM |
denotes |
− |
T4237 |
4734-4735 |
-LRB- |
denotes |
( |
T4238 |
4742-4744 |
NN |
denotes |
2C |
T4239 |
4735-4741 |
NN |
denotes |
Figure |
T4240 |
4745-4748 |
CC |
denotes |
and |
T4241 |
4749-4751 |
NN |
denotes |
2F |
T4242 |
4751-4752 |
-RRB- |
denotes |
) |
T4243 |
4752-4753 |
. |
denotes |
. |
T4244 |
4753-4911 |
sentence |
denotes |
Within the ventral horn, Ia afferents in both wild-type and Er81EWS-Pea3/− mice formed vGlut1+ terminals that were absent in Er81 mutant mice (Figure 2G–2I). |
T4245 |
4754-4760 |
IN |
denotes |
Within |
T4246 |
4834-4840 |
VBD |
denotes |
formed |
T4247 |
4761-4764 |
DT |
denotes |
the |
T4248 |
4773-4777 |
NN |
denotes |
horn |
T4249 |
4765-4772 |
JJ |
denotes |
ventral |
T4250 |
4777-4779 |
, |
denotes |
, |
T4251 |
4779-4781 |
NN |
denotes |
Ia |
T4252 |
4782-4791 |
NNS |
denotes |
afferents |
T4253 |
4792-4794 |
IN |
denotes |
in |
T4254 |
4795-4799 |
CC |
denotes |
both |
T4255 |
4805-4809 |
NN |
denotes |
type |
T4256 |
4800-4804 |
JJ |
denotes |
wild |
T4257 |
4804-4805 |
HYPH |
denotes |
- |
T4258 |
4829-4833 |
NNS |
denotes |
mice |
T4259 |
4810-4813 |
CC |
denotes |
and |
T4260 |
4814-4821 |
NN |
denotes |
Er81EWS |
T4261 |
4822-4826 |
NN |
denotes |
Pea3 |
T4262 |
4821-4822 |
HYPH |
denotes |
- |
T4263 |
4826-4827 |
HYPH |
denotes |
/ |
T4264 |
4827-4828 |
SYM |
denotes |
− |
T4265 |
4841-4847 |
NN |
denotes |
vGlut1 |
T4266 |
4849-4858 |
NNS |
denotes |
terminals |
T4267 |
4847-4848 |
SYM |
denotes |
+ |
T4268 |
4859-4863 |
WDT |
denotes |
that |
T4269 |
4864-4868 |
VBD |
denotes |
were |
T4270 |
4869-4875 |
JJ |
denotes |
absent |
T4271 |
4876-4878 |
IN |
denotes |
in |
T4272 |
4879-4883 |
NN |
denotes |
Er81 |
T4273 |
4891-4895 |
NNS |
denotes |
mice |
T4274 |
4884-4890 |
NN |
denotes |
mutant |
T4275 |
4896-4897 |
-LRB- |
denotes |
( |
T4276 |
4904-4906 |
NN |
denotes |
2G |
T4277 |
4897-4903 |
NN |
denotes |
Figure |
T4278 |
4906-4907 |
SYM |
denotes |
– |
T4279 |
4907-4909 |
NN |
denotes |
2I |
T4280 |
4909-4910 |
-RRB- |
denotes |
) |
T4281 |
4910-4911 |
. |
denotes |
. |
T4282 |
4911-5164 |
sentence |
denotes |
To assess whether synapses between Ia afferents and motor neurons are functional in Er81EWS-Pea3/− mice, we performed intracellular recordings from identified quadriceps motor neurons after stimulation of nerves innervating the quadriceps muscle group. |
T4283 |
4912-4914 |
TO |
denotes |
To |
T4284 |
4915-4921 |
VB |
denotes |
assess |
T4285 |
5020-5029 |
VBD |
denotes |
performed |
T4286 |
4922-4929 |
IN |
denotes |
whether |
T4287 |
4978-4981 |
VBP |
denotes |
are |
T4288 |
4930-4938 |
NNS |
denotes |
synapses |
T4289 |
4939-4946 |
IN |
denotes |
between |
T4290 |
4947-4949 |
NN |
denotes |
Ia |
T4291 |
4950-4959 |
NNS |
denotes |
afferents |
T4292 |
4960-4963 |
CC |
denotes |
and |
T4293 |
4964-4969 |
NN |
denotes |
motor |
T4294 |
4970-4977 |
NNS |
denotes |
neurons |
T4295 |
4982-4992 |
JJ |
denotes |
functional |
T4296 |
4993-4995 |
IN |
denotes |
in |
T4297 |
4996-5003 |
NN |
denotes |
Er81EWS |
T4298 |
5004-5008 |
NN |
denotes |
Pea3 |
T4299 |
5003-5004 |
HYPH |
denotes |
- |
T4300 |
5011-5015 |
NNS |
denotes |
mice |
T4301 |
5008-5009 |
HYPH |
denotes |
/ |
T4302 |
5009-5010 |
SYM |
denotes |
− |
T4303 |
5015-5017 |
, |
denotes |
, |
T4304 |
5017-5019 |
PRP |
denotes |
we |
T4305 |
5030-5043 |
JJ |
denotes |
intracellular |
T4306 |
5044-5054 |
NNS |
denotes |
recordings |
T4307 |
5055-5059 |
IN |
denotes |
from |
T4308 |
5060-5070 |
VBN |
denotes |
identified |
T4309 |
5088-5095 |
NNS |
denotes |
neurons |
T4310 |
5071-5081 |
NNS |
denotes |
quadriceps |
T4311 |
5082-5087 |
NN |
denotes |
motor |
T4312 |
5096-5101 |
IN |
denotes |
after |
T4313 |
5102-5113 |
NN |
denotes |
stimulation |
T4314 |
5114-5116 |
IN |
denotes |
of |
T4315 |
5117-5123 |
NNS |
denotes |
nerves |
T4316 |
5124-5135 |
VBG |
denotes |
innervating |
T4317 |
5136-5139 |
DT |
denotes |
the |
T4318 |
5158-5163 |
NN |
denotes |
group |
T4319 |
5140-5150 |
NNS |
denotes |
quadriceps |
T4320 |
5151-5157 |
NN |
denotes |
muscle |
T4321 |
5163-5164 |
. |
denotes |
. |
T4322 |
5164-5381 |
sentence |
denotes |
We found no significant difference in the input amplitude to quadriceps motor neurons when comparing wild-type to Er81EWS-Pea3/− mice (Figure S2; wild-type, 10.6 ± 0.9 mV, n = 11; Er81EWS-Pea3/−, 10.9 ± 1 mV, n = 8). |
T4323 |
5165-5167 |
PRP |
denotes |
We |
T4324 |
5168-5173 |
VBD |
denotes |
found |
T4325 |
5174-5176 |
DT |
denotes |
no |
T4326 |
5189-5199 |
NN |
denotes |
difference |
T4327 |
5177-5188 |
JJ |
denotes |
significant |
T4328 |
5200-5202 |
IN |
denotes |
in |
T4329 |
5203-5206 |
DT |
denotes |
the |
T4330 |
5213-5222 |
NN |
denotes |
amplitude |
T4331 |
5207-5212 |
NN |
denotes |
input |
T4332 |
5223-5225 |
IN |
denotes |
to |
T4333 |
5226-5236 |
NNS |
denotes |
quadriceps |
T4334 |
5243-5250 |
NNS |
denotes |
neurons |
T4335 |
5237-5242 |
NN |
denotes |
motor |
T4336 |
5251-5255 |
WRB |
denotes |
when |
T4337 |
5256-5265 |
VBG |
denotes |
comparing |
T4338 |
5266-5270 |
JJ |
denotes |
wild |
T4339 |
5271-5275 |
NN |
denotes |
type |
T4340 |
5270-5271 |
HYPH |
denotes |
- |
T4341 |
5276-5278 |
IN |
denotes |
to |
T4342 |
5279-5286 |
NN |
denotes |
Er81EWS |
T4343 |
5287-5291 |
NN |
denotes |
Pea3 |
T4344 |
5286-5287 |
HYPH |
denotes |
- |
T4345 |
5291-5292 |
HYPH |
denotes |
/ |
T4346 |
5292-5293 |
SYM |
denotes |
− |
T4347 |
5294-5298 |
NNS |
denotes |
mice |
T4348 |
5299-5300 |
-LRB- |
denotes |
( |
T4349 |
5307-5309 |
NN |
denotes |
S2 |
T4350 |
5300-5306 |
NN |
denotes |
Figure |
T4351 |
5309-5310 |
: |
denotes |
; |
T4352 |
5311-5315 |
JJ |
denotes |
wild |
T4353 |
5316-5320 |
NN |
denotes |
type |
T4354 |
5315-5316 |
HYPH |
denotes |
- |
T4355 |
5341-5343 |
CD |
denotes |
11 |
T4356 |
5320-5322 |
, |
denotes |
, |
T4357 |
5322-5326 |
CD |
denotes |
10.6 |
T4358 |
5329-5332 |
CD |
denotes |
0.9 |
T4359 |
5327-5328 |
SYM |
denotes |
± |
T4360 |
5333-5335 |
NN |
denotes |
mV |
T4361 |
5335-5337 |
, |
denotes |
, |
T4362 |
5337-5338 |
NN |
denotes |
n |
T4363 |
5339-5340 |
SYM |
denotes |
= |
T4364 |
5343-5344 |
: |
denotes |
; |
T4365 |
5345-5352 |
NN |
denotes |
Er81EWS |
T4366 |
5353-5357 |
NN |
denotes |
Pea3 |
T4367 |
5352-5353 |
HYPH |
denotes |
- |
T4368 |
5378-5379 |
CD |
denotes |
8 |
T4369 |
5357-5358 |
HYPH |
denotes |
/ |
T4370 |
5358-5359 |
SYM |
denotes |
− |
T4371 |
5359-5361 |
, |
denotes |
, |
T4372 |
5361-5365 |
CD |
denotes |
10.9 |
T4373 |
5368-5369 |
CD |
denotes |
1 |
T4374 |
5366-5367 |
SYM |
denotes |
± |
T4375 |
5370-5372 |
NN |
denotes |
mV |
T4376 |
5372-5374 |
, |
denotes |
, |
T4377 |
5374-5375 |
NN |
denotes |
n |
T4378 |
5376-5377 |
SYM |
denotes |
= |
T4379 |
5379-5380 |
-RRB- |
denotes |
) |
T4380 |
5380-5381 |
. |
denotes |
. |
T4381 |
5381-5732 |
sentence |
denotes |
Together, these findings suggest that in the absence of Er81, EWS-Pea3 can direct the complex biological process of correct laminar termination within the ventral spinal cord and the formation of synapses with motor neurons (Figure 2J–2L), thus identifying an ETS transcription factor suitable for heterochronic expression experiments in DRG neurons. |
T4382 |
5382-5390 |
RB |
denotes |
Together |
T4383 |
5407-5414 |
VBP |
denotes |
suggest |
T4384 |
5390-5392 |
, |
denotes |
, |
T4385 |
5392-5397 |
DT |
denotes |
these |
T4386 |
5398-5406 |
NNS |
denotes |
findings |
T4387 |
5415-5419 |
IN |
denotes |
that |
T4388 |
5457-5463 |
VB |
denotes |
direct |
T4389 |
5420-5422 |
IN |
denotes |
in |
T4390 |
5423-5426 |
DT |
denotes |
the |
T4391 |
5427-5434 |
NN |
denotes |
absence |
T4392 |
5435-5437 |
IN |
denotes |
of |
T4393 |
5438-5442 |
NN |
denotes |
Er81 |
T4394 |
5442-5444 |
, |
denotes |
, |
T4395 |
5444-5447 |
NN |
denotes |
EWS |
T4396 |
5448-5452 |
NN |
denotes |
Pea3 |
T4397 |
5447-5448 |
HYPH |
denotes |
- |
T4398 |
5453-5456 |
MD |
denotes |
can |
T4399 |
5464-5467 |
DT |
denotes |
the |
T4400 |
5487-5494 |
NN |
denotes |
process |
T4401 |
5468-5475 |
JJ |
denotes |
complex |
T4402 |
5476-5486 |
JJ |
denotes |
biological |
T4403 |
5495-5497 |
IN |
denotes |
of |
T4404 |
5498-5505 |
JJ |
denotes |
correct |
T4405 |
5514-5525 |
NN |
denotes |
termination |
T4406 |
5506-5513 |
JJ |
denotes |
laminar |
T4407 |
5526-5532 |
IN |
denotes |
within |
T4408 |
5533-5536 |
DT |
denotes |
the |
T4409 |
5552-5556 |
NN |
denotes |
cord |
T4410 |
5537-5544 |
JJ |
denotes |
ventral |
T4411 |
5545-5551 |
JJ |
denotes |
spinal |
T4412 |
5557-5560 |
CC |
denotes |
and |
T4413 |
5561-5564 |
DT |
denotes |
the |
T4414 |
5565-5574 |
NN |
denotes |
formation |
T4415 |
5575-5577 |
IN |
denotes |
of |
T4416 |
5578-5586 |
NNS |
denotes |
synapses |
T4417 |
5587-5591 |
IN |
denotes |
with |
T4418 |
5592-5597 |
NN |
denotes |
motor |
T4419 |
5598-5605 |
NNS |
denotes |
neurons |
T4420 |
5606-5607 |
-LRB- |
denotes |
( |
T4421 |
5614-5616 |
NN |
denotes |
2J |
T4422 |
5607-5613 |
NN |
denotes |
Figure |
T4423 |
5616-5617 |
SYM |
denotes |
– |
T4424 |
5617-5619 |
NN |
denotes |
2L |
T4425 |
5619-5620 |
-RRB- |
denotes |
) |
T4426 |
5620-5622 |
, |
denotes |
, |
T4427 |
5622-5626 |
RB |
denotes |
thus |
T4428 |
5627-5638 |
VBG |
denotes |
identifying |
T4429 |
5639-5641 |
DT |
denotes |
an |
T4430 |
5660-5666 |
NN |
denotes |
factor |
T4431 |
5642-5645 |
NN |
denotes |
ETS |
T4432 |
5646-5659 |
NN |
denotes |
transcription |
T4433 |
5667-5675 |
JJ |
denotes |
suitable |
T4434 |
5676-5679 |
IN |
denotes |
for |
T4435 |
5680-5693 |
JJ |
denotes |
heterochronic |
T4436 |
5705-5716 |
NNS |
denotes |
experiments |
T4437 |
5694-5704 |
NN |
denotes |
expression |
T4438 |
5717-5719 |
IN |
denotes |
in |
T4439 |
5720-5723 |
NN |
denotes |
DRG |
T4440 |
5724-5731 |
NNS |
denotes |
neurons |
T4441 |
5731-5732 |
. |
denotes |
. |
R999 |
T3609 |
T3610 |
compound |
EWS,Pea3 |
R1000 |
T3610 |
T3612 |
nsubj |
Pea3,Replace |
R1001 |
T3611 |
T3610 |
punct |
-,Pea3 |
R1002 |
T3613 |
T3612 |
aux |
Can,Replace |
R1003 |
T3614 |
T3615 |
compound |
Er81,Function |
R1004 |
T3615 |
T3612 |
dobj |
Function,Replace |
R1005 |
T3616 |
T3612 |
prep |
in,Replace |
R1006 |
T3617 |
T3616 |
pcomp |
Controlling,in |
R1007 |
T3618 |
T3619 |
nmod |
Ia,Projections |
R1008 |
T3619 |
T3617 |
dobj |
Projections,Controlling |
R1009 |
T3620 |
T3619 |
amod |
Afferent,Projections |
R1010 |
T3622 |
T3623 |
nsubj |
We,defined |
R1011 |
T3624 |
T3623 |
advmod |
first,defined |
R1012 |
T3625 |
T3626 |
det |
an,regulator |
R1013 |
T3626 |
T3623 |
dobj |
regulator,defined |
R1014 |
T3627 |
T3626 |
compound |
ETS,regulator |
R1015 |
T3628 |
T3626 |
compound |
transcription,regulator |
R1016 |
T3629 |
T3630 |
dep |
that,is |
R1017 |
T3630 |
T3626 |
relcl |
is,regulator |
R1018 |
T3631 |
T3630 |
acomp |
able,is |
R1019 |
T3632 |
T3633 |
aux |
to,replace |
R1020 |
T3633 |
T3631 |
xcomp |
replace,able |
R1021 |
T3634 |
T3635 |
det |
the,function |
R1022 |
T3635 |
T3633 |
dobj |
function,replace |
R1023 |
T3636 |
T3635 |
prep |
of,function |
R1024 |
T3637 |
T3636 |
pobj |
Er81,of |
R1025 |
T3638 |
T3635 |
prep |
within,function |
R1026 |
T3639 |
T3640 |
amod |
proprioceptive,afferents |
R1027 |
T3640 |
T3638 |
pobj |
afferents,within |
R1028 |
T3641 |
T3642 |
aux |
to,direct |
R1029 |
T3642 |
T3633 |
advcl |
direct,replace |
R1030 |
T3643 |
T3642 |
dobj |
projections,direct |
R1031 |
T3644 |
T3642 |
prep |
into,direct |
R1032 |
T3645 |
T3646 |
det |
the,cord |
R1033 |
T3646 |
T3644 |
pobj |
cord,into |
R1034 |
T3647 |
T3646 |
amod |
ventral,cord |
R1035 |
T3648 |
T3646 |
amod |
spinal,cord |
R1036 |
T3649 |
T3623 |
punct |
.,defined |
R1037 |
T3651 |
T3652 |
nsubj |
Er81,constitute |
R1038 |
T3653 |
T3651 |
punct |
", ",Er81 |
R1039 |
T3654 |
T3651 |
conj |
Pea3,Er81 |
R1040 |
T3655 |
T3654 |
punct |
", ",Pea3 |
R1041 |
T3656 |
T3654 |
cc |
and,Pea3 |
R1042 |
T3657 |
T3654 |
conj |
Erm,Pea3 |
R1043 |
T3658 |
T3659 |
det |
the,subfamily |
R1044 |
T3659 |
T3652 |
dobj |
subfamily,constitute |
R1045 |
T3660 |
T3659 |
compound |
Pea3,subfamily |
R1046 |
T3661 |
T3659 |
prep |
of,subfamily |
R1047 |
T3662 |
T3663 |
compound |
ETS,factors |
R1048 |
T3663 |
T3661 |
pobj |
factors,of |
R1049 |
T3664 |
T3663 |
compound |
transcription,factors |
R1050 |
T3665 |
T3652 |
punct |
", ",constitute |
R1051 |
T3666 |
T3652 |
conj |
show,constitute |
R1052 |
T3667 |
T3668 |
det |
a,degree |
R1053 |
T3668 |
T3666 |
dobj |
degree,show |
R1054 |
T3669 |
T3668 |
amod |
high,degree |
R1055 |
T3670 |
T3668 |
prep |
of,degree |
R1056 |
T3671 |
T3672 |
compound |
amino,acid |
R1057 |
T3672 |
T3673 |
compound |
acid,identity |
R1058 |
T3673 |
T3670 |
pobj |
identity,of |
R1059 |
T3674 |
T3666 |
punct |
", ",show |
R1060 |
T3675 |
T3666 |
cc |
and,show |
R1061 |
T3676 |
T3666 |
conj |
bind,show |
R1062 |
T3677 |
T3676 |
prep |
to,bind |
R1063 |
T3678 |
T3679 |
advmod |
very,similar |
R1064 |
T3679 |
T3680 |
amod |
similar,sequences |
R1065 |
T3680 |
T3677 |
pobj |
sequences,to |
R1066 |
T3681 |
T3680 |
compound |
DNA,sequences |
R1067 |
T3682 |
T3680 |
compound |
target,sequences |
R1068 |
T3683 |
T3684 |
punct |
[,19 |
R1069 |
T3684 |
T3676 |
parataxis |
19,bind |
R1070 |
T3685 |
T3684 |
nummod |
17,19 |
R1071 |
T3686 |
T3684 |
punct |
",",19 |
R1072 |
T3687 |
T3684 |
nummod |
18,19 |
R1073 |
T3688 |
T3684 |
punct |
",",19 |
R1074 |
T3689 |
T3684 |
punct |
],19 |
R1075 |
T3690 |
T3652 |
punct |
.,constitute |
R1076 |
T3692 |
T3693 |
advmod |
Nevertheless,rescue |
R1077 |
T3694 |
T3693 |
punct |
", ",rescue |
R1078 |
T3695 |
T3696 |
advmod |
when,introduced |
R1079 |
T3696 |
T3693 |
advcl |
introduced,rescue |
R1080 |
T3697 |
T3696 |
prep |
into,introduced |
R1081 |
T3698 |
T3699 |
det |
the,locus |
R1082 |
T3699 |
T3697 |
pobj |
locus,into |
R1083 |
T3700 |
T3699 |
compound |
Er81,locus |
R1084 |
T3701 |
T3696 |
prep |
in,introduced |
R1085 |
T3702 |
T3701 |
pobj |
analogy,in |
R1086 |
T3703 |
T3702 |
prep |
to,analogy |
R1087 |
T3704 |
T3705 |
det |
a,strategy |
R1088 |
T3705 |
T3703 |
pobj |
strategy,to |
R1089 |
T3706 |
T3707 |
advmod |
previously,used |
R1090 |
T3707 |
T3705 |
amod |
used,strategy |
R1091 |
T3708 |
T3705 |
compound |
targeting,strategy |
R1092 |
T3709 |
T3710 |
punct |
(,shown |
R1093 |
T3710 |
T3696 |
parataxis |
shown,introduced |
R1094 |
T3711 |
T3710 |
nsubj |
data,shown |
R1095 |
T3712 |
T3710 |
neg |
not,shown |
R1096 |
T3713 |
T3710 |
punct |
;,shown |
R1097 |
T3714 |
T3715 |
punct |
[,14 |
R1098 |
T3715 |
T3710 |
parataxis |
14,shown |
R1099 |
T3716 |
T3715 |
punct |
],14 |
R1100 |
T3717 |
T3710 |
punct |
),shown |
R1101 |
T3718 |
T3693 |
punct |
", ",rescue |
R1102 |
T3719 |
T3720 |
preconj |
neither,Pea3 |
R1103 |
T3720 |
T3693 |
nsubj |
Pea3,rescue |
R1104 |
T3721 |
T3720 |
cc |
nor,Pea3 |
R1105 |
T3722 |
T3720 |
conj |
Erm,Pea3 |
R1106 |
T3723 |
T3693 |
aux |
could,rescue |
R1107 |
T3724 |
T3725 |
npadvmod |
Ia,proprioceptive |
R1108 |
T3725 |
T3726 |
amod |
proprioceptive,projections |
R1109 |
T3726 |
T3693 |
dobj |
projections,rescue |
R1110 |
T3727 |
T3726 |
amod |
afferent,projections |
R1111 |
T3728 |
T3729 |
aux |
to,invade |
R1112 |
T3729 |
T3693 |
advcl |
invade,rescue |
R1113 |
T3730 |
T3729 |
advmod |
extensively,invade |
R1114 |
T3731 |
T3732 |
det |
the,horn |
R1115 |
T3732 |
T3729 |
dobj |
horn,invade |
R1116 |
T3733 |
T3732 |
amod |
ventral,horn |
R1117 |
T3734 |
T3732 |
prep |
of,horn |
R1118 |
T3735 |
T3736 |
det |
the,cord |
R1119 |
T3736 |
T3734 |
pobj |
cord,of |
R1120 |
T3737 |
T3736 |
amod |
spinal,cord |
R1121 |
T3738 |
T3739 |
punct |
(,shown |
R1122 |
T3739 |
T3729 |
parataxis |
shown,invade |
R1123 |
T3740 |
T3739 |
nsubj |
data,shown |
R1124 |
T3741 |
T3739 |
neg |
not,shown |
R1125 |
T3742 |
T3739 |
punct |
),shown |
R1126 |
T3743 |
T3693 |
punct |
.,rescue |
R1127 |
T3745 |
T3746 |
det |
These,findings |
R1128 |
T3746 |
T3747 |
nsubj |
findings,prompted |
R1129 |
T3748 |
T3747 |
dobj |
us,prompted |
R1130 |
T3749 |
T3750 |
aux |
to,analyze |
R1131 |
T3750 |
T3747 |
xcomp |
analyze,prompted |
R1132 |
T3751 |
T3750 |
dobj |
mice,analyze |
R1133 |
T3752 |
T3753 |
prep |
in,integrated |
R1134 |
T3753 |
T3751 |
relcl |
integrated,mice |
R1135 |
T3754 |
T3752 |
pobj |
which,in |
R1136 |
T3755 |
T3753 |
nsubj |
we,integrated |
R1137 |
T3756 |
T3757 |
compound |
EWS,Pea3 |
R1138 |
T3757 |
T3753 |
dobj |
Pea3,integrated |
R1139 |
T3758 |
T3757 |
punct |
-,Pea3 |
R1140 |
T3759 |
T3757 |
punct |
", ",Pea3 |
R1141 |
T3760 |
T3761 |
det |
a,product |
R1142 |
T3761 |
T3757 |
appos |
product,Pea3 |
R1143 |
T3762 |
T3763 |
compound |
break,point |
R1144 |
T3763 |
T3761 |
compound |
point,product |
R1145 |
T3764 |
T3763 |
punct |
-,point |
R1146 |
T3765 |
T3761 |
compound |
fusion,product |
R1147 |
T3766 |
T3761 |
prep |
between,product |
R1148 |
T3767 |
T3768 |
det |
the,domain |
R1149 |
T3768 |
T3766 |
pobj |
domain,between |
R1150 |
T3769 |
T3770 |
npadvmod |
amino,terminal |
R1151 |
T3770 |
T3768 |
amod |
terminal,domain |
R1152 |
T3771 |
T3770 |
punct |
-,terminal |
R1153 |
T3772 |
T3768 |
prep |
of,domain |
R1154 |
T3773 |
T3774 |
det |
the,gene |
R1155 |
T3774 |
T3772 |
pobj |
gene,of |
R1156 |
T3775 |
T3776 |
nmod |
Ewing,sarcoma |
R1157 |
T3776 |
T3774 |
nmod |
sarcoma,gene |
R1158 |
T3777 |
T3776 |
punct |
(,sarcoma |
R1159 |
T3778 |
T3776 |
appos |
EWS,sarcoma |
R1160 |
T3779 |
T3774 |
punct |
),gene |
R1161 |
T3780 |
T3768 |
cc |
and,domain |
R1162 |
T3781 |
T3782 |
det |
the,domain |
R1163 |
T3782 |
T3768 |
conj |
domain,domain |
R1164 |
T3783 |
T3784 |
compound |
Pea3,DNA |
R1165 |
T3784 |
T3785 |
npadvmod |
DNA,binding |
R1166 |
T3785 |
T3782 |
amod |
binding,domain |
R1167 |
T3786 |
T3787 |
punct |
[,21 |
R1168 |
T3787 |
T3782 |
parataxis |
21,domain |
R1169 |
T3788 |
T3787 |
nummod |
20,21 |
R1170 |
T3789 |
T3787 |
punct |
",",21 |
R1171 |
T3790 |
T3787 |
punct |
],21 |
R1172 |
T3791 |
T3753 |
punct |
", ",integrated |
R1173 |
T3792 |
T3753 |
prep |
into,integrated |
R1174 |
T3793 |
T3794 |
det |
the,locus |
R1175 |
T3794 |
T3792 |
pobj |
locus,into |
R1176 |
T3795 |
T3794 |
compound |
Er81,locus |
R1177 |
T3796 |
T3797 |
punct |
(,Figure |
R1178 |
T3797 |
T3753 |
parataxis |
Figure,integrated |
R1179 |
T3798 |
T3797 |
nummod |
1,Figure |
R1180 |
T3799 |
T3797 |
punct |
),Figure |
R1181 |
T3800 |
T3747 |
punct |
.,prompted |
R1182 |
T3802 |
T3803 |
nsubj |
We,found |
R1183 |
T3804 |
T3805 |
mark |
that,showed |
R1184 |
T3805 |
T3803 |
ccomp |
showed,found |
R1185 |
T3806 |
T3805 |
prep |
in,showed |
R1186 |
T3807 |
T3808 |
det |
a,assay |
R1187 |
T3808 |
T3806 |
pobj |
assay,in |
R1188 |
T3809 |
T3810 |
compound |
luciferase,enzyme |
R1189 |
T3810 |
T3812 |
npadvmod |
enzyme,based |
R1190 |
T3811 |
T3810 |
punct |
-,enzyme |
R1191 |
T3812 |
T3808 |
amod |
based,assay |
R1192 |
T3813 |
T3812 |
punct |
-,based |
R1193 |
T3814 |
T3815 |
compound |
cell,culture |
R1194 |
T3815 |
T3808 |
compound |
culture,assay |
R1195 |
T3816 |
T3808 |
compound |
transfection,assay |
R1196 |
T3817 |
T3805 |
punct |
", ",showed |
R1197 |
T3818 |
T3819 |
compound |
EWS,Pea3 |
R1198 |
T3819 |
T3805 |
nsubj |
Pea3,showed |
R1199 |
T3820 |
T3819 |
punct |
-,Pea3 |
R1200 |
T3821 |
T3822 |
amod |
stronger,activity |
R1201 |
T3822 |
T3805 |
dobj |
activity,showed |
R1202 |
T3823 |
T3822 |
compound |
transactivation,activity |
R1203 |
T3824 |
T3822 |
prep |
than,activity |
R1204 |
T3825 |
T3824 |
pobj |
Er81,than |
R1205 |
T3826 |
T3825 |
cc |
or,Er81 |
R1206 |
T3827 |
T3825 |
conj |
Pea3,Er81 |
R1207 |
T3828 |
T3829 |
punct |
(,1J |
R1208 |
T3829 |
T3822 |
parataxis |
1J,activity |
R1209 |
T3830 |
T3829 |
compound |
Figure,1J |
R1210 |
T3831 |
T3829 |
punct |
;,1J |
R1211 |
T3832 |
T3833 |
nsubj |
data,shown |
R1212 |
T3833 |
T3829 |
ccomp |
shown,1J |
R1213 |
T3834 |
T3833 |
neg |
not,shown |
R1214 |
T3835 |
T3829 |
punct |
),1J |
R1215 |
T3836 |
T3805 |
punct |
", ",showed |
R1216 |
T3837 |
T3805 |
prep |
in,showed |
R1217 |
T3838 |
T3837 |
pobj |
agreement,in |
R1218 |
T3839 |
T3838 |
prep |
with,agreement |
R1219 |
T3840 |
T3841 |
amod |
previous,studies |
R1220 |
T3841 |
T3839 |
pobj |
studies,with |
R1221 |
T3842 |
T3843 |
punct |
[,24 |
R1222 |
T3843 |
T3841 |
parataxis |
24,studies |
R1223 |
T3844 |
T3843 |
nummod |
22,24 |
R1224 |
T3845 |
T3843 |
punct |
",",24 |
R1225 |
T3846 |
T3843 |
nummod |
23,24 |
R1226 |
T3847 |
T3843 |
punct |
",",24 |
R1227 |
T3848 |
T3843 |
punct |
],24 |
R1228 |
T3849 |
T3803 |
punct |
.,found |
R1229 |
T3851 |
T3852 |
advmod |
Moreover,abolished |
R1230 |
T3853 |
T3852 |
punct |
", ",abolished |
R1231 |
T3854 |
T3852 |
nsubjpass |
transactivation,abolished |
R1232 |
T3855 |
T3854 |
prep |
by,transactivation |
R1233 |
T3856 |
T3857 |
compound |
EWS,Pea3 |
R1234 |
T3857 |
T3855 |
pobj |
Pea3,by |
R1235 |
T3858 |
T3857 |
punct |
-,Pea3 |
R1236 |
T3859 |
T3852 |
auxpass |
was,abolished |
R1237 |
T3860 |
T3852 |
agent |
by,abolished |
R1238 |
T3861 |
T3860 |
pobj |
mutation,by |
R1239 |
T3862 |
T3861 |
prep |
of,mutation |
R1240 |
T3863 |
T3864 |
npadvmod |
ETS,binding |
R1241 |
T3864 |
T3866 |
amod |
binding,sites |
R1242 |
T3865 |
T3864 |
punct |
-,binding |
R1243 |
T3866 |
T3862 |
pobj |
sites,of |
R1244 |
T3867 |
T3861 |
prep |
in,mutation |
R1245 |
T3868 |
T3869 |
det |
the,plasmid |
R1246 |
T3869 |
T3867 |
pobj |
plasmid,in |
R1247 |
T3870 |
T3869 |
compound |
reporter,plasmid |
R1248 |
T3871 |
T3852 |
punct |
", ",abolished |
R1249 |
T3872 |
T3852 |
advcl |
demonstrating,abolished |
R1250 |
T3873 |
T3874 |
npadvmod |
ETS,binding |
R1251 |
T3874 |
T3876 |
amod |
binding,site |
R1252 |
T3875 |
T3874 |
punct |
-,binding |
R1253 |
T3876 |
T3878 |
compound |
site,dependence |
R1254 |
T3877 |
T3876 |
punct |
-,site |
R1255 |
T3878 |
T3872 |
dobj |
dependence,demonstrating |
R1256 |
T3879 |
T3880 |
punct |
(,shown |
R1257 |
T3880 |
T3872 |
parataxis |
shown,demonstrating |
R1258 |
T3881 |
T3880 |
nsubj |
data,shown |
R1259 |
T3882 |
T3880 |
neg |
not,shown |
R1260 |
T3883 |
T3880 |
punct |
),shown |
R1261 |
T3884 |
T3852 |
punct |
.,abolished |
R1262 |
T3886 |
T3887 |
nsubjpass |
Expression,abolished |
R1263 |
T3888 |
T3886 |
prep |
of,Expression |
R1264 |
T3889 |
T3888 |
pobj |
Er81,of |
R1265 |
T3890 |
T3886 |
prep |
in,Expression |
R1266 |
T3891 |
T3892 |
compound |
DRG,neurons |
R1267 |
T3892 |
T3890 |
pobj |
neurons,in |
R1268 |
T3893 |
T3892 |
prep |
of,neurons |
R1269 |
T3894 |
T3893 |
pobj |
embryos,of |
R1270 |
T3895 |
T3894 |
acl |
containing,embryos |
R1271 |
T3896 |
T3895 |
dobj |
integration,containing |
R1272 |
T3897 |
T3896 |
prep |
of,integration |
R1273 |
T3898 |
T3899 |
compound |
EWS,Pea3 |
R1274 |
T3899 |
T3897 |
pobj |
Pea3,of |
R1275 |
T3900 |
T3899 |
punct |
-,Pea3 |
R1276 |
T3901 |
T3886 |
prep |
in,Expression |
R1277 |
T3902 |
T3903 |
det |
the,locus |
R1278 |
T3903 |
T3901 |
pobj |
locus,in |
R1279 |
T3904 |
T3903 |
compound |
Er81,locus |
R1280 |
T3905 |
T3903 |
punct |
(,locus |
R1281 |
T3906 |
T3907 |
compound |
Er81EWS,Pea3 |
R1282 |
T3907 |
T3903 |
appos |
Pea3,locus |
R1283 |
T3908 |
T3907 |
punct |
-,Pea3 |
R1284 |
T3909 |
T3907 |
punct |
/,Pea3 |
R1285 |
T3910 |
T3907 |
punct |
−,Pea3 |
R1286 |
T3911 |
T3887 |
punct |
),abolished |
R1287 |
T3912 |
T3887 |
auxpass |
was,abolished |
R1288 |
T3913 |
T3914 |
punct |
(,1E |
R1289 |
T3914 |
T3887 |
parataxis |
1E,abolished |
R1290 |
T3915 |
T3914 |
compound |
Figure,1E |
R1291 |
T3916 |
T3914 |
punct |
),1E |
R1292 |
T3917 |
T3887 |
punct |
", ",abolished |
R1293 |
T3918 |
T3887 |
cc |
and,abolished |
R1294 |
T3919 |
T3920 |
det |
the,level |
R1295 |
T3920 |
T3922 |
nsubj |
level,was |
R1296 |
T3921 |
T3920 |
compound |
expression,level |
R1297 |
T3922 |
T3887 |
conj |
was,abolished |
R1298 |
T3923 |
T3920 |
prep |
of,level |
R1299 |
T3924 |
T3925 |
det |
the,protein |
R1300 |
T3925 |
T3923 |
pobj |
protein,of |
R1301 |
T3926 |
T3927 |
npadvmod |
calcium,binding |
R1302 |
T3927 |
T3925 |
amod |
binding,protein |
R1303 |
T3928 |
T3927 |
punct |
-,binding |
R1304 |
T3929 |
T3925 |
appos |
Parvalbumin,protein |
R1305 |
T3930 |
T3929 |
punct |
(,Parvalbumin |
R1306 |
T3931 |
T3929 |
appos |
PV,Parvalbumin |
R1307 |
T3932 |
T3920 |
punct |
),level |
R1308 |
T3933 |
T3920 |
prep |
in,level |
R1309 |
T3934 |
T3935 |
amod |
proprioceptive,afferents |
R1310 |
T3935 |
T3933 |
pobj |
afferents,in |
R1311 |
T3936 |
T3920 |
punct |
", ",level |
R1312 |
T3937 |
T3938 |
dep |
which,decreased |
R1313 |
T3938 |
T3920 |
relcl |
decreased,level |
R1314 |
T3939 |
T3938 |
auxpass |
is,decreased |
R1315 |
T3940 |
T3941 |
advmod |
approximately,10 |
R1316 |
T3941 |
T3945 |
quantmod |
10,fold |
R1317 |
T3942 |
T3941 |
quantmod |
5,10 |
R1318 |
T3943 |
T3941 |
punct |
-,10 |
R1319 |
T3944 |
T3941 |
quantmod |
to,10 |
R1320 |
T3945 |
T3938 |
advmod |
fold,decreased |
R1321 |
T3946 |
T3945 |
punct |
-,fold |
R1322 |
T3947 |
T3938 |
prep |
in,decreased |
R1323 |
T3948 |
T3949 |
compound |
Er81,mutants |
R1324 |
T3949 |
T3947 |
pobj |
mutants,in |
R1325 |
T3950 |
T3951 |
punct |
[,14 |
R1326 |
T3951 |
T3938 |
parataxis |
14,decreased |
R1327 |
T3952 |
T3951 |
punct |
],14 |
R1328 |
T3953 |
T3922 |
punct |
", ",was |
R1329 |
T3954 |
T3922 |
acomp |
comparable,was |
R1330 |
T3955 |
T3954 |
prep |
to,comparable |
R1331 |
T3956 |
T3957 |
amod |
wild,type |
R1332 |
T3957 |
T3959 |
compound |
type,levels |
R1333 |
T3958 |
T3957 |
punct |
-,type |
R1334 |
T3959 |
T3955 |
pobj |
levels,to |
R1335 |
T3960 |
T3922 |
prep |
in,was |
R1336 |
T3961 |
T3962 |
nmod |
Er81EWS,Pea3 |
R1337 |
T3962 |
T3964 |
nmod |
Pea3,embryos |
R1338 |
T3963 |
T3962 |
punct |
-,Pea3 |
R1339 |
T3964 |
T3960 |
pobj |
embryos,in |
R1340 |
T3965 |
T3962 |
punct |
/,Pea3 |
R1341 |
T3966 |
T3962 |
punct |
−,Pea3 |
R1342 |
T3967 |
T3968 |
punct |
(,1F |
R1343 |
T3968 |
T3922 |
parataxis |
1F,was |
R1344 |
T3969 |
T3968 |
compound |
Figure,1F |
R1345 |
T3970 |
T3971 |
punct |
–,1H |
R1346 |
T3971 |
T3968 |
prep |
1H,1F |
R1347 |
T3972 |
T3968 |
punct |
),1F |
R1348 |
T3973 |
T3922 |
punct |
.,was |
R1349 |
T3975 |
T3976 |
aux |
To,define |
R1350 |
T3976 |
T3978 |
advcl |
define,assessed |
R1351 |
T3977 |
T3976 |
advmod |
further,define |
R1352 |
T3979 |
T3980 |
compound |
DRG,neuron |
R1353 |
T3980 |
T3981 |
compound |
neuron,differentiation |
R1354 |
T3981 |
T3976 |
dobj |
differentiation,define |
R1355 |
T3982 |
T3976 |
prep |
in,define |
R1356 |
T3983 |
T3984 |
det |
the,presence |
R1357 |
T3984 |
T3982 |
pobj |
presence,in |
R1358 |
T3985 |
T3984 |
prep |
of,presence |
R1359 |
T3986 |
T3987 |
compound |
EWS,Pea3 |
R1360 |
T3987 |
T3985 |
pobj |
Pea3,of |
R1361 |
T3988 |
T3987 |
punct |
-,Pea3 |
R1362 |
T3989 |
T3976 |
prep |
in,define |
R1363 |
T3990 |
T3991 |
amod |
proprioceptive,afferents |
R1364 |
T3991 |
T3989 |
pobj |
afferents,in |
R1365 |
T3992 |
T3993 |
advmod |
in,vivo |
R1366 |
T3993 |
T3976 |
advmod |
vivo,define |
R1367 |
T3994 |
T3978 |
punct |
", ",assessed |
R1368 |
T3995 |
T3978 |
nsubj |
we,assessed |
R1369 |
T3996 |
T3997 |
mark |
whether,had |
R1370 |
T3997 |
T3978 |
ccomp |
had,assessed |
R1371 |
T3998 |
T3997 |
nsubj |
replacement,had |
R1372 |
T3999 |
T3998 |
prep |
of,replacement |
R1373 |
T4000 |
T3999 |
pobj |
Er81,of |
R1374 |
T4001 |
T3998 |
prep |
by,replacement |
R1375 |
T4002 |
T4003 |
compound |
EWS,Pea3 |
R1376 |
T4003 |
T4001 |
pobj |
Pea3,by |
R1377 |
T4004 |
T4003 |
punct |
-,Pea3 |
R1378 |
T4005 |
T4006 |
det |
an,influence |
R1379 |
T4006 |
T3997 |
dobj |
influence,had |
R1380 |
T4007 |
T4006 |
prep |
on,influence |
R1381 |
T4008 |
T4009 |
amod |
neuronal,survival |
R1382 |
T4009 |
T4007 |
pobj |
survival,on |
R1383 |
T4010 |
T4007 |
cc |
or,on |
R1384 |
T4011 |
T4007 |
conj |
on,on |
R1385 |
T4012 |
T4013 |
det |
the,expression |
R1386 |
T4013 |
T4011 |
pobj |
expression,on |
R1387 |
T4014 |
T4013 |
prep |
of,expression |
R1388 |
T4015 |
T4016 |
amod |
proprioceptive,afferent |
R1389 |
T4016 |
T4018 |
amod |
afferent,specific |
R1390 |
T4017 |
T4016 |
punct |
-,afferent |
R1391 |
T4018 |
T4020 |
amod |
specific,genes |
R1392 |
T4019 |
T4018 |
punct |
-,specific |
R1393 |
T4020 |
T4014 |
pobj |
genes,of |
R1394 |
T4021 |
T3978 |
punct |
.,assessed |
R1395 |
T4023 |
T4024 |
nmod |
Er81EWS,Pea3 |
R1396 |
T4024 |
T4026 |
nmod |
Pea3,mice |
R1397 |
T4025 |
T4024 |
punct |
-,Pea3 |
R1398 |
T4026 |
T4029 |
nsubj |
mice,differ |
R1399 |
T4027 |
T4024 |
punct |
/,Pea3 |
R1400 |
T4028 |
T4024 |
punct |
−,Pea3 |
R1401 |
T4030 |
T4029 |
aux |
did,differ |
R1402 |
T4031 |
T4029 |
neg |
not,differ |
R1403 |
T4032 |
T4029 |
prep |
from,differ |
R1404 |
T4033 |
T4034 |
amod |
wild,type |
R1405 |
T4034 |
T4032 |
pobj |
type,from |
R1406 |
T4035 |
T4034 |
punct |
-,type |
R1407 |
T4036 |
T4029 |
prep |
in,differ |
R1408 |
T4037 |
T4038 |
det |
the,number |
R1409 |
T4038 |
T4036 |
pobj |
number,in |
R1410 |
T4039 |
T4038 |
prep |
of,number |
R1411 |
T4040 |
T4041 |
amod |
proprioceptive,bodies |
R1412 |
T4041 |
T4039 |
pobj |
bodies,of |
R1413 |
T4042 |
T4041 |
amod |
afferent,bodies |
R1414 |
T4043 |
T4041 |
compound |
cell,bodies |
R1415 |
T4044 |
T4041 |
prep |
within,bodies |
R1416 |
T4045 |
T4046 |
det |
the,DRG |
R1417 |
T4046 |
T4044 |
pobj |
DRG,within |
R1418 |
T4047 |
T4046 |
prep |
of,DRG |
R1419 |
T4048 |
T4047 |
pobj |
L1,of |
R1420 |
T4049 |
T4048 |
prep |
to,L1 |
R1421 |
T4050 |
T4049 |
pobj |
L5,to |
R1422 |
T4051 |
T4038 |
punct |
", ",number |
R1423 |
T4052 |
T4053 |
det |
the,expression |
R1424 |
T4053 |
T4038 |
conj |
expression,number |
R1425 |
T4054 |
T4053 |
prep |
of,expression |
R1426 |
T4055 |
T4056 |
amod |
several,genes |
R1427 |
T4056 |
T4054 |
pobj |
genes,of |
R1428 |
T4057 |
T4058 |
advmod |
normally,expressed |
R1429 |
T4058 |
T4053 |
acl |
expressed,expression |
R1430 |
T4059 |
T4058 |
agent |
by,expressed |
R1431 |
T4060 |
T4061 |
amod |
proprioceptive,afferents |
R1432 |
T4061 |
T4059 |
pobj |
afferents,by |
R1433 |
T4062 |
T4053 |
punct |
", ",expression |
R1434 |
T4063 |
T4053 |
cc |
and,expression |
R1435 |
T4064 |
T4065 |
det |
the,lack |
R1436 |
T4065 |
T4053 |
conj |
lack,expression |
R1437 |
T4066 |
T4065 |
prep |
of,lack |
R1438 |
T4067 |
T4066 |
pobj |
expression,of |
R1439 |
T4068 |
T4067 |
prep |
of,expression |
R1440 |
T4069 |
T4068 |
pobj |
genes,of |
R1441 |
T4070 |
T4071 |
neg |
not,expressed |
R1442 |
T4071 |
T4069 |
acl |
expressed,genes |
R1443 |
T4072 |
T4071 |
advmod |
normally,expressed |
R1444 |
T4073 |
T4071 |
prep |
in,expressed |
R1445 |
T4074 |
T4075 |
amod |
proprioceptive,afferents |
R1446 |
T4075 |
T4073 |
pobj |
afferents,in |
R1447 |
T4076 |
T4077 |
punct |
(,S1 |
R1448 |
T4077 |
T4029 |
parataxis |
S1,differ |
R1449 |
T4078 |
T4077 |
compound |
Figure,S1 |
R1450 |
T4079 |
T4077 |
punct |
;,S1 |
R1451 |
T4080 |
T4081 |
nsubj |
data,shown |
R1452 |
T4081 |
T4077 |
ccomp |
shown,S1 |
R1453 |
T4082 |
T4081 |
neg |
not,shown |
R1454 |
T4083 |
T4077 |
punct |
),S1 |
R1455 |
T4084 |
T4029 |
punct |
.,differ |
R1456 |
T4086 |
T4087 |
advmod |
Together,suggest |
R1457 |
T4088 |
T4087 |
punct |
", ",suggest |
R1458 |
T4089 |
T4090 |
det |
these,findings |
R1459 |
T4090 |
T4087 |
nsubj |
findings,suggest |
R1460 |
T4091 |
T4092 |
mark |
that,mimics |
R1461 |
T4092 |
T4087 |
ccomp |
mimics,suggest |
R1462 |
T4093 |
T4094 |
det |
the,expression |
R1463 |
T4094 |
T4092 |
nsubj |
expression,mimics |
R1464 |
T4095 |
T4094 |
prep |
of,expression |
R1465 |
T4096 |
T4097 |
compound |
EWS,Pea3 |
R1466 |
T4097 |
T4095 |
pobj |
Pea3,of |
R1467 |
T4098 |
T4097 |
punct |
-,Pea3 |
R1468 |
T4099 |
T4094 |
prep |
from,expression |
R1469 |
T4100 |
T4101 |
det |
the,time |
R1470 |
T4101 |
T4099 |
pobj |
time,from |
R1471 |
T4102 |
T4101 |
amod |
normal,time |
R1472 |
T4103 |
T4101 |
prep |
of,time |
R1473 |
T4104 |
T4103 |
pobj |
onset,of |
R1474 |
T4105 |
T4106 |
det |
the,function |
R1475 |
T4106 |
T4092 |
dobj |
function,mimics |
R1476 |
T4107 |
T4106 |
prep |
of,function |
R1477 |
T4108 |
T4107 |
pobj |
Er81,of |
R1478 |
T4109 |
T4110 |
mark |
as,assessed |
R1479 |
T4110 |
T4092 |
advcl |
assessed,mimics |
R1480 |
T4111 |
T4110 |
prep |
by,assessed |
R1481 |
T4112 |
T4111 |
pobj |
induction,by |
R1482 |
T4113 |
T4112 |
cc |
and,induction |
R1483 |
T4114 |
T4112 |
conj |
maintenance,induction |
R1484 |
T4115 |
T4112 |
prep |
of,induction |
R1485 |
T4116 |
T4117 |
compound |
gene,expression |
R1486 |
T4117 |
T4115 |
pobj |
expression,of |
R1487 |
T4118 |
T4112 |
prep |
within,induction |
R1488 |
T4119 |
T4120 |
amod |
proprioceptive,afferents |
R1489 |
T4120 |
T4118 |
pobj |
afferents,within |
R1490 |
T4121 |
T4087 |
punct |
.,suggest |
R1491 |
T4123 |
T4124 |
aux |
To,determine |
R1492 |
T4124 |
T4125 |
advcl |
determine,traced |
R1493 |
T4126 |
T4127 |
det |
the,extent |
R1494 |
T4127 |
T4124 |
dobj |
extent,determine |
R1495 |
T4128 |
T4127 |
prep |
of,extent |
R1496 |
T4129 |
T4128 |
pobj |
rescue,of |
R1497 |
T4130 |
T4129 |
prep |
of,rescue |
R1498 |
T4131 |
T4132 |
nmod |
Ia,projections |
R1499 |
T4132 |
T4130 |
pobj |
projections,of |
R1500 |
T4133 |
T4132 |
amod |
proprioceptive,projections |
R1501 |
T4134 |
T4132 |
amod |
afferent,projections |
R1502 |
T4135 |
T4132 |
prep |
into,projections |
R1503 |
T4136 |
T4137 |
det |
the,cord |
R1504 |
T4137 |
T4135 |
pobj |
cord,into |
R1505 |
T4138 |
T4137 |
amod |
ventral,cord |
R1506 |
T4139 |
T4137 |
amod |
spinal,cord |
R1507 |
T4140 |
T4137 |
prep |
of,cord |
R1508 |
T4141 |
T4142 |
compound |
Er81,mice |
R1509 |
T4142 |
T4140 |
pobj |
mice,of |
R1510 |
T4143 |
T4142 |
compound |
mutant,mice |
R1511 |
T4144 |
T4132 |
acl |
achieved,projections |
R1512 |
T4145 |
T4144 |
agent |
by,achieved |
R1513 |
T4146 |
T4145 |
pobj |
expression,by |
R1514 |
T4147 |
T4146 |
prep |
of,expression |
R1515 |
T4148 |
T4149 |
compound |
EWS,Pea3 |
R1516 |
T4149 |
T4147 |
pobj |
Pea3,of |
R1517 |
T4150 |
T4149 |
punct |
-,Pea3 |
R1518 |
T4151 |
T4125 |
punct |
", ",traced |
R1519 |
T4152 |
T4125 |
nsubj |
we,traced |
R1520 |
T4153 |
T4154 |
amod |
intraspinal,projections |
R1521 |
T4154 |
T4125 |
dobj |
projections,traced |
R1522 |
T4155 |
T4154 |
amod |
afferent,projections |
R1523 |
T4156 |
T4125 |
prep |
by,traced |
R1524 |
T4157 |
T4158 |
amod |
axonal,labeling |
R1525 |
T4158 |
T4156 |
pobj |
labeling,by |
R1526 |
T4265 |
T4266 |
nmod |
vGlut1,terminals |
R1527 |
T4266 |
T4246 |
dobj |
terminals,formed |
R1528 |
T4267 |
T4265 |
punct |
+,vGlut1 |
R1529 |
T4268 |
T4269 |
dep |
that,were |
R1530 |
T4159 |
T4158 |
prep |
of,labeling |
R1531 |
T4269 |
T4266 |
relcl |
were,terminals |
R1532 |
T4160 |
T4159 |
pobj |
PV,of |
R1533 |
T4270 |
T4269 |
acomp |
absent,were |
R1534 |
T4161 |
T4162 |
punct |
(,2A |
R1535 |
T4162 |
T4125 |
parataxis |
2A,traced |
R1536 |
T4271 |
T4269 |
prep |
in,were |
R1537 |
T4163 |
T4162 |
compound |
Figure,2A |
R1538 |
T4164 |
T4165 |
punct |
–,2C |
R1539 |
T4165 |
T4162 |
prep |
2C,2A |
R1540 |
T4272 |
T4273 |
compound |
Er81,mice |
R1541 |
T4166 |
T4162 |
punct |
),2A |
R1542 |
T4167 |
T4125 |
punct |
.,traced |
R1543 |
T4273 |
T4271 |
pobj |
mice,in |
R1544 |
T4169 |
T4170 |
prep |
In,used |
R1545 |
T4274 |
T4273 |
compound |
mutant,mice |
R1546 |
T4171 |
T4169 |
pobj |
addition,In |
R1547 |
T4172 |
T4170 |
punct |
", ",used |
R1548 |
T4275 |
T4276 |
punct |
(,2G |
R1549 |
T4173 |
T4174 |
aux |
to,analyze |
R1550 |
T4174 |
T4170 |
advcl |
analyze,used |
R1551 |
T4276 |
T4269 |
parataxis |
2G,were |
R1552 |
T4175 |
T4176 |
compound |
axon,ingrowth |
R1553 |
T4176 |
T4174 |
dobj |
ingrowth,analyze |
R1554 |
T4177 |
T4176 |
amod |
independent,ingrowth |
R1555 |
T4277 |
T4276 |
compound |
Figure,2G |
R1556 |
T4178 |
T4177 |
prep |
of,independent |
R1557 |
T4179 |
T4180 |
det |
the,level |
R1558 |
T4180 |
T4178 |
pobj |
level,of |
R1559 |
T4278 |
T4279 |
punct |
–,2I |
R1560 |
T4181 |
T4180 |
prep |
of,level |
R1561 |
T4182 |
T4183 |
compound |
PV,expression |
R1562 |
T4279 |
T4276 |
prep |
2I,2G |
R1563 |
T4183 |
T4181 |
pobj |
expression,of |
R1564 |
T4184 |
T4180 |
prep |
in,level |
R1565 |
T4185 |
T4186 |
compound |
DRG,neurons |
R1566 |
T4186 |
T4184 |
pobj |
neurons,in |
R1567 |
T4187 |
T4170 |
punct |
", ",used |
R1568 |
T4280 |
T4276 |
punct |
),2G |
R1569 |
T4188 |
T4170 |
nsubj |
we,used |
R1570 |
T4189 |
T4190 |
amod |
anterograde,labeling |
R1571 |
T4190 |
T4170 |
dobj |
labeling,used |
R1572 |
T4281 |
T4246 |
punct |
.,formed |
R1573 |
T4191 |
T4190 |
prep |
of,labeling |
R1574 |
T4192 |
T4193 |
amod |
afferent,fibers |
R1575 |
T4283 |
T4284 |
aux |
To,assess |
R1576 |
T4193 |
T4191 |
pobj |
fibers,of |
R1577 |
T4284 |
T4285 |
advcl |
assess,performed |
R1578 |
T4194 |
T4170 |
prep |
by,used |
R1579 |
T4286 |
T4287 |
mark |
whether,are |
R1580 |
T4195 |
T4194 |
pcomp |
applying,by |
R1581 |
T4196 |
T4197 |
advmod |
fluorescently,labeled |
R1582 |
T4197 |
T4198 |
amod |
labeled,dextran |
R1583 |
T4198 |
T4195 |
dobj |
dextran,applying |
R1584 |
T4199 |
T4195 |
prep |
to,applying |
R1585 |
T4287 |
T4284 |
ccomp |
are,assess |
R1586 |
T4200 |
T4201 |
amod |
cut,roots |
R1587 |
T4201 |
T4199 |
pobj |
roots,to |
R1588 |
T4202 |
T4201 |
amod |
dorsal,roots |
R1589 |
T4288 |
T4287 |
nsubj |
synapses,are |
R1590 |
T4203 |
T4204 |
punct |
(,2D |
R1591 |
T4204 |
T4195 |
parataxis |
2D,applying |
R1592 |
T4205 |
T4204 |
compound |
Figure,2D |
R1593 |
T4289 |
T4288 |
prep |
between,synapses |
R1594 |
T4206 |
T4207 |
punct |
–,2F |
R1595 |
T4207 |
T4204 |
prep |
2F,2D |
R1596 |
T4208 |
T4204 |
punct |
),2D |
R1597 |
T4290 |
T4291 |
compound |
Ia,afferents |
R1598 |
T4209 |
T4170 |
punct |
.,used |
R1599 |
T4291 |
T4289 |
pobj |
afferents,between |
R1600 |
T4211 |
T4212 |
advcl |
Using,found |
R1601 |
T4213 |
T4214 |
det |
both,assays |
R1602 |
T4292 |
T4291 |
cc |
and,afferents |
R1603 |
T4214 |
T4211 |
dobj |
assays,Using |
R1604 |
T4293 |
T4294 |
compound |
motor,neurons |
R1605 |
T4215 |
T4212 |
punct |
", ",found |
R1606 |
T4294 |
T4291 |
conj |
neurons,afferents |
R1607 |
T4216 |
T4212 |
nsubj |
we,found |
R1608 |
T4217 |
T4218 |
amod |
extensive,rescue |
R1609 |
T4218 |
T4212 |
dobj |
rescue,found |
R1610 |
T4295 |
T4287 |
acomp |
functional,are |
R1611 |
T4219 |
T4218 |
prep |
of,rescue |
R1612 |
T4220 |
T4221 |
det |
the,projections |
R1613 |
T4296 |
T4287 |
prep |
in,are |
R1614 |
T4221 |
T4219 |
pobj |
projections,of |
R1615 |
T4222 |
T4221 |
prep |
into,projections |
R1616 |
T4223 |
T4224 |
det |
the,horn |
R1617 |
T4224 |
T4222 |
pobj |
horn,into |
R1618 |
T4225 |
T4224 |
amod |
ventral,horn |
R1619 |
T4297 |
T4298 |
nmod |
Er81EWS,Pea3 |
R1620 |
T4226 |
T4224 |
prep |
of,horn |
R1621 |
T4227 |
T4228 |
det |
the,cord |
R1622 |
T4228 |
T4226 |
pobj |
cord,of |
R1623 |
T4298 |
T4300 |
nmod |
Pea3,mice |
R1624 |
T4229 |
T4228 |
amod |
spinal,cord |
R1625 |
T4230 |
T4212 |
prep |
in,found |
R1626 |
T4299 |
T4298 |
punct |
-,Pea3 |
R1627 |
T4231 |
T4232 |
nmod |
Er81EWS,Pea3 |
R1628 |
T4232 |
T4234 |
nmod |
Pea3,mice |
R1629 |
T4233 |
T4232 |
punct |
-,Pea3 |
R1630 |
T4300 |
T4296 |
pobj |
mice,in |
R1631 |
T4234 |
T4230 |
pobj |
mice,in |
R1632 |
T4235 |
T4232 |
punct |
/,Pea3 |
R1633 |
T4236 |
T4232 |
punct |
−,Pea3 |
R1634 |
T4301 |
T4298 |
punct |
/,Pea3 |
R1635 |
T4237 |
T4238 |
punct |
(,2C |
R1636 |
T4238 |
T4212 |
parataxis |
2C,found |
R1637 |
T4239 |
T4238 |
compound |
Figure,2C |
R1638 |
T4302 |
T4298 |
punct |
−,Pea3 |
R1639 |
T4240 |
T4238 |
cc |
and,2C |
R1640 |
T4241 |
T4238 |
conj |
2F,2C |
R1641 |
T4242 |
T4238 |
punct |
),2C |
R1642 |
T4303 |
T4285 |
punct |
", ",performed |
R1643 |
T4243 |
T4212 |
punct |
.,found |
R1644 |
T4304 |
T4285 |
nsubj |
we,performed |
R1645 |
T4245 |
T4246 |
prep |
Within,formed |
R1646 |
T4247 |
T4248 |
det |
the,horn |
R1647 |
T4305 |
T4306 |
amod |
intracellular,recordings |
R1648 |
T4248 |
T4245 |
pobj |
horn,Within |
R1649 |
T4249 |
T4248 |
amod |
ventral,horn |
R1650 |
T4250 |
T4246 |
punct |
", ",formed |
R1651 |
T4306 |
T4285 |
dobj |
recordings,performed |
R1652 |
T4251 |
T4252 |
compound |
Ia,afferents |
R1653 |
T4252 |
T4246 |
nsubj |
afferents,formed |
R1654 |
T4253 |
T4252 |
prep |
in,afferents |
R1655 |
T4307 |
T4306 |
prep |
from,recordings |
R1656 |
T4254 |
T4255 |
preconj |
both,type |
R1657 |
T4255 |
T4258 |
nmod |
type,mice |
R1658 |
T4256 |
T4255 |
amod |
wild,type |
R1659 |
T4257 |
T4255 |
punct |
-,type |
R1660 |
T4308 |
T4309 |
amod |
identified,neurons |
R1661 |
T4258 |
T4253 |
pobj |
mice,in |
R1662 |
T4259 |
T4255 |
cc |
and,type |
R1663 |
T4260 |
T4261 |
compound |
Er81EWS,Pea3 |
R1664 |
T4309 |
T4307 |
pobj |
neurons,from |
R1665 |
T4261 |
T4255 |
conj |
Pea3,type |
R1666 |
T4262 |
T4261 |
punct |
-,Pea3 |
R1667 |
T4263 |
T4261 |
punct |
/,Pea3 |
R1668 |
T4264 |
T4261 |
punct |
−,Pea3 |
R1669 |
T4310 |
T4309 |
compound |
quadriceps,neurons |
R1670 |
T4311 |
T4309 |
compound |
motor,neurons |
R1671 |
T4312 |
T4285 |
prep |
after,performed |
R1672 |
T4313 |
T4312 |
pobj |
stimulation,after |
R1673 |
T4314 |
T4313 |
prep |
of,stimulation |
R1674 |
T4371 |
T4368 |
punct |
", ",8 |
R1675 |
T4315 |
T4314 |
pobj |
nerves,of |
R1676 |
T4372 |
T4373 |
quantmod |
10.9,1 |
R1677 |
T4373 |
T4375 |
nummod |
1,mV |
R1678 |
T4316 |
T4315 |
acl |
innervating,nerves |
R1679 |
T4374 |
T4373 |
punct |
±,1 |
R1680 |
T4375 |
T4368 |
dep |
mV,8 |
R1681 |
T4376 |
T4368 |
punct |
", ",8 |
R1682 |
T4317 |
T4318 |
det |
the,group |
R1683 |
T4377 |
T4368 |
nsubj |
n,8 |
R1684 |
T4378 |
T4368 |
punct |
=,8 |
R1685 |
T4379 |
T4349 |
punct |
),S2 |
R1686 |
T4318 |
T4316 |
dobj |
group,innervating |
R1687 |
T4380 |
T4324 |
punct |
.,found |
R1688 |
T4382 |
T4383 |
advmod |
Together,suggest |
R1689 |
T4319 |
T4318 |
compound |
quadriceps,group |
R1690 |
T4384 |
T4383 |
punct |
", ",suggest |
R1691 |
T4320 |
T4318 |
compound |
muscle,group |
R1692 |
T4321 |
T4285 |
punct |
.,performed |
R1693 |
T4385 |
T4386 |
det |
these,findings |
R1694 |
T4386 |
T4383 |
nsubj |
findings,suggest |
R1695 |
T4323 |
T4324 |
nsubj |
We,found |
R1696 |
T4387 |
T4388 |
mark |
that,direct |
R1697 |
T4388 |
T4383 |
ccomp |
direct,suggest |
R1698 |
T4389 |
T4388 |
prep |
in,direct |
R1699 |
T4325 |
T4326 |
det |
no,difference |
R1700 |
T4390 |
T4391 |
det |
the,absence |
R1701 |
T4391 |
T4389 |
pobj |
absence,in |
R1702 |
T4392 |
T4391 |
prep |
of,absence |
R1703 |
T4393 |
T4392 |
pobj |
Er81,of |
R1704 |
T4326 |
T4324 |
dobj |
difference,found |
R1705 |
T4327 |
T4326 |
amod |
significant,difference |
R1706 |
T4328 |
T4326 |
prep |
in,difference |
R1707 |
T4329 |
T4330 |
det |
the,amplitude |
R1708 |
T4394 |
T4388 |
punct |
", ",direct |
R1709 |
T4330 |
T4328 |
pobj |
amplitude,in |
R1710 |
T4395 |
T4396 |
compound |
EWS,Pea3 |
R1711 |
T4396 |
T4388 |
nsubj |
Pea3,direct |
R1712 |
T4331 |
T4330 |
compound |
input,amplitude |
R1713 |
T4397 |
T4396 |
punct |
-,Pea3 |
R1714 |
T4398 |
T4388 |
aux |
can,direct |
R1715 |
T4332 |
T4330 |
prep |
to,amplitude |
R1716 |
T4399 |
T4400 |
det |
the,process |
R1717 |
T4400 |
T4388 |
dobj |
process,direct |
R1718 |
T4401 |
T4400 |
amod |
complex,process |
R1719 |
T4333 |
T4334 |
compound |
quadriceps,neurons |
R1720 |
T4402 |
T4400 |
amod |
biological,process |
R1721 |
T4403 |
T4400 |
prep |
of,process |
R1722 |
T4404 |
T4405 |
amod |
correct,termination |
R1723 |
T4405 |
T4403 |
pobj |
termination,of |
R1724 |
T4406 |
T4405 |
amod |
laminar,termination |
R1725 |
T4407 |
T4405 |
prep |
within,termination |
R1726 |
T4334 |
T4332 |
pobj |
neurons,to |
R1727 |
T4408 |
T4409 |
det |
the,cord |
R1728 |
T4409 |
T4407 |
pobj |
cord,within |
R1729 |
T4410 |
T4409 |
amod |
ventral,cord |
R1730 |
T4335 |
T4334 |
compound |
motor,neurons |
R1731 |
T4411 |
T4409 |
amod |
spinal,cord |
R1732 |
T4412 |
T4400 |
cc |
and,process |
R1733 |
T4413 |
T4414 |
det |
the,formation |
R1734 |
T4336 |
T4337 |
advmod |
when,comparing |
R1735 |
T4414 |
T4400 |
conj |
formation,process |
R1736 |
T4415 |
T4414 |
prep |
of,formation |
R1737 |
T4416 |
T4415 |
pobj |
synapses,of |
R1738 |
T4337 |
T4324 |
advcl |
comparing,found |
R1739 |
T4417 |
T4416 |
prep |
with,synapses |
R1740 |
T4418 |
T4419 |
compound |
motor,neurons |
R1741 |
T4338 |
T4339 |
amod |
wild,type |
R1742 |
T4419 |
T4417 |
pobj |
neurons,with |
R1743 |
T4420 |
T4421 |
punct |
(,2J |
R1744 |
T4339 |
T4337 |
dobj |
type,comparing |
R1745 |
T4421 |
T4388 |
parataxis |
2J,direct |
R1746 |
T4422 |
T4421 |
compound |
Figure,2J |
R1747 |
T4423 |
T4424 |
punct |
–,2L |
R1748 |
T4340 |
T4339 |
punct |
-,type |
R1749 |
T4424 |
T4421 |
prep |
2L,2J |
R1750 |
T4425 |
T4421 |
punct |
),2J |
R1751 |
T4426 |
T4388 |
punct |
", ",direct |
R1752 |
T4341 |
T4337 |
prep |
to,comparing |
R1753 |
T4427 |
T4428 |
advmod |
thus,identifying |
R1754 |
T4428 |
T4388 |
advcl |
identifying,direct |
R1755 |
T4429 |
T4430 |
det |
an,factor |
R1756 |
T4342 |
T4343 |
compound |
Er81EWS,Pea3 |
R1757 |
T4430 |
T4428 |
dobj |
factor,identifying |
R1758 |
T4431 |
T4430 |
compound |
ETS,factor |
R1759 |
T4343 |
T4341 |
pobj |
Pea3,to |
R1760 |
T4432 |
T4430 |
compound |
transcription,factor |
R1761 |
T4433 |
T4430 |
amod |
suitable,factor |
R1762 |
T4434 |
T4433 |
prep |
for,suitable |
R1763 |
T4344 |
T4343 |
punct |
-,Pea3 |
R1764 |
T4435 |
T4436 |
amod |
heterochronic,experiments |
R1765 |
T4436 |
T4434 |
pobj |
experiments,for |
R1766 |
T4437 |
T4436 |
compound |
expression,experiments |
R1767 |
T4345 |
T4343 |
punct |
/,Pea3 |
R1768 |
T4438 |
T4436 |
prep |
in,experiments |
R1769 |
T4439 |
T4440 |
compound |
DRG,neurons |
R1770 |
T4440 |
T4438 |
pobj |
neurons,in |
R1771 |
T4346 |
T4343 |
punct |
−,Pea3 |
R1772 |
T4441 |
T4383 |
punct |
.,suggest |
R1773 |
T4347 |
T4341 |
pobj |
mice,to |
R1774 |
T4348 |
T4349 |
punct |
(,S2 |
R1775 |
T4349 |
T4337 |
parataxis |
S2,comparing |
R1776 |
T4350 |
T4349 |
compound |
Figure,S2 |
R1777 |
T4351 |
T4349 |
punct |
;,S2 |
R1778 |
T4352 |
T4353 |
amod |
wild,type |
R1779 |
T4353 |
T4355 |
dep |
type,11 |
R1780 |
T4354 |
T4353 |
punct |
-,type |
R1781 |
T4355 |
T4349 |
dep |
11,S2 |
R1782 |
T4356 |
T4355 |
punct |
", ",11 |
R1783 |
T4357 |
T4358 |
quantmod |
10.6,0.9 |
R1784 |
T4358 |
T4360 |
nummod |
0.9,mV |
R1785 |
T4359 |
T4358 |
punct |
±,0.9 |
R1786 |
T4360 |
T4355 |
dep |
mV,11 |
R1787 |
T4361 |
T4355 |
punct |
", ",11 |
R1788 |
T4362 |
T4355 |
nsubj |
n,11 |
R1789 |
T4363 |
T4355 |
punct |
=,11 |
R1790 |
T4364 |
T4349 |
punct |
;,S2 |
R1791 |
T4365 |
T4366 |
compound |
Er81EWS,Pea3 |
R1792 |
T4366 |
T4368 |
dep |
Pea3,8 |
R1793 |
T4367 |
T4366 |
punct |
-,Pea3 |
R1794 |
T4368 |
T4349 |
dep |
8,S2 |
R1795 |
T4369 |
T4366 |
punct |
/,Pea3 |
R1796 |
T4370 |
T4366 |
punct |
−,Pea3 |