PMC:1084331 / 49854-50668 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/1084331","sourcedb":"PMC","sourceid":"1084331","source_url":"http://www.ncbi.nlm.nih.gov/pmc/1084331","text":"Generation of PVCre Mice\n(A) Above is the organization of the PV genomic locus. Exons are schematically illustrated as light blue boxes, where exon 2 contains the start codon (ATG) and exon 5 contains the stop codon (STOP). Probe to screen for homologous recombination is shown as grey box. Below is a schematic diagram to show the PV locus after the integration of an IRES-Cre cassette (green) 3′ to the translational stop codon of PV using homologous recombination in ES cells.\n(B) Southern blot analysis of PVCre wild-type (+/+) and heterozygous (+/−) genomic DNA using the probe indicated in (A).\n(C and D) Expression of GFP (green) and LacZ (red; C) or PV (red; D) in P0 TaumGFP/+ PVCre/+ mice. Note that more than 90% of PV+ neurons coexpress GFP (D; data not shown).\nScale bar: (C and D), 50 μm.\n(2 MB CDR).","divisions":[{"label":"title","span":{"begin":0,"end":24}},{"label":"p","span":{"begin":25,"end":479}},{"label":"p","span":{"begin":480,"end":600}},{"label":"p","span":{"begin":601,"end":773}},{"label":"p","span":{"begin":774,"end":802}}],"tracks":[]}