PMC:1084331 / 48257-49110 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/1084331","sourcedb":"PMC","sourceid":"1084331","source_url":"http://www.ncbi.nlm.nih.gov/pmc/1084331","text":"(A) Top panel shows organization of the Tau genomic locus in the region targeted by homologous recombination in analogy to Tucker et al. [41]. Exons 1–3 are shown as light blue boxes, and the Tau start codon in exon 2 is indicated as ATG. The probe used to detect homologous recombination is shown as a grey box. Middle and bottom panels show Tau locus after homologous recombination to integrate targeting cassettes (green) into exon 2 with coincident elimination the endogenous Tau start codon. The integrated targeting cassettes allow for conditional expression of EWS-Pea3 and NLS-LacZ (NLZ) (middle) or mGFP and NLS-LacZ (bottom) upon Cre-recombinase-mediated activation. In the absence of Cre recombinase, a transcriptional stop sequence flanked by loxP sites inhibits expression of the respective transgenes from their start codons (ATG in grey).","tracks":[{"project":"2_test","denotations":[{"id":"15836427-11135642-84719690","span":{"begin":138,"end":140},"obj":"11135642"},{"id":"T56896","span":{"begin":138,"end":140},"obj":"11135642"}],"attributes":[{"subj":"15836427-11135642-84719690","pred":"source","obj":"2_test"},{"subj":"T56896","pred":"source","obj":"2_test"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"2_test","color":"#939dec","default":true}]}]}}