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DRG explants (n ≥ 20 for each condition) were cultured for 48 h, processed for immunocytochemistry, and analyzed using confocal microscopy."}

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lumbar DRG were dissected from E13.5 or E14.5 embryos and placed on Matrigel (BD Biosciences, San Jose, California, United States) coated coverslips in DMEM/F12 (Gibco, San Diego, California, United States), 2 mM L-Gln (Gibco), N2 (Gibco), and 1 mg/ml BSA (Sigma, St. Louis, Missouri, United States) without neurotrophins, or supplemented with either NGF (100 ng/ml, Gibco) or NT-3 (20 ng/ml, Sigma). DRG explants (n ≥ 20 for each condition) were cultured for 48 h, processed for immunocytochemistry, and analyzed using confocal microscopy."}

{"project":"craft-ca-core-dev","denotations":[{"id":"T14454","span":{"begin":11,"end":21},"obj":"UBERON:0002836"},{"id":"T14455","span":{"begin":57,"end":64},"obj":"UBERON:0000922"},{"id":"T14456","span":{"begin":319,"end":332},"obj":"PR:000021998"},{"id":"T14457","span":{"begin":362,"end":365},"obj":"PR:000011194"},{"id":"T14458","span":{"begin":388,"end":392},"obj":"PR:000011459"},{"id":"T14459","span":{"begin":412,"end":415},"obj":"UBERON:0000044"}],"text":"Individual lumbar DRG were dissected from E13.5 or E14.5 embryos and placed on Matrigel (BD Biosciences, San Jose, California, United States) coated coverslips in DMEM/F12 (Gibco, San Diego, California, United States), 2 mM L-Gln (Gibco), N2 (Gibco), and 1 mg/ml BSA (Sigma, St. Louis, Missouri, United States) without neurotrophins, or supplemented with either NGF (100 ng/ml, Gibco) or NT-3 (20 ng/ml, Sigma). DRG explants (n ≥ 20 for each condition) were cultured for 48 h, processed for immunocytochemistry, and analyzed using confocal microscopy."}