Id |
Subject |
Object |
Predicate |
Lexical cue |
T13917 |
0-2 |
FW |
denotes |
In |
T13918 |
3-7 |
FW |
denotes |
situ |
T13919 |
8-21 |
NN |
denotes |
hybridization |
T13920 |
22-25 |
CC |
denotes |
and |
T13921 |
26-46 |
NN |
denotes |
immunohistochemistry |
T13922 |
46-229 |
sentence |
denotes |
For in situ hybridization analysis, cryostat sections were hybridized using digoxigenin-labeled probes [45] directed against mouse TrkA or TrkB, or rat TrkC (gift from L. F. Parada). |
T13923 |
47-50 |
IN |
denotes |
For |
T13924 |
106-116 |
VBN |
denotes |
hybridized |
T13925 |
51-53 |
FW |
denotes |
in |
T13926 |
54-58 |
FW |
denotes |
situ |
T13927 |
73-81 |
NN |
denotes |
analysis |
T13928 |
59-72 |
NN |
denotes |
hybridization |
T13929 |
81-83 |
, |
denotes |
, |
T13930 |
83-91 |
NN |
denotes |
cryostat |
T13931 |
92-100 |
NNS |
denotes |
sections |
T13932 |
101-105 |
VBD |
denotes |
were |
T13933 |
117-122 |
VBG |
denotes |
using |
T13934 |
123-134 |
NN |
denotes |
digoxigenin |
T13935 |
135-142 |
VBN |
denotes |
labeled |
T13936 |
134-135 |
HYPH |
denotes |
- |
T13937 |
143-149 |
NNS |
denotes |
probes |
T13938 |
150-151 |
-LRB- |
denotes |
[ |
T13939 |
151-153 |
CD |
denotes |
45 |
T13940 |
153-154 |
-RRB- |
denotes |
] |
T13941 |
155-163 |
VBN |
denotes |
directed |
T13942 |
164-171 |
IN |
denotes |
against |
T13943 |
172-177 |
NN |
denotes |
mouse |
T13944 |
178-182 |
NN |
denotes |
TrkA |
T13945 |
183-185 |
CC |
denotes |
or |
T13946 |
186-190 |
NN |
denotes |
TrkB |
T13947 |
190-192 |
, |
denotes |
, |
T13948 |
192-194 |
CC |
denotes |
or |
T13949 |
195-198 |
NN |
denotes |
rat |
T13950 |
199-203 |
NN |
denotes |
TrkC |
T13951 |
204-205 |
-LRB- |
denotes |
( |
T13952 |
205-209 |
NN |
denotes |
gift |
T13953 |
210-214 |
IN |
denotes |
from |
T13954 |
215-217 |
NNP |
denotes |
L. |
T13955 |
221-227 |
NNP |
denotes |
Parada |
T13956 |
218-220 |
NNP |
denotes |
F. |
T13957 |
227-228 |
-RRB- |
denotes |
) |
T13958 |
228-229 |
. |
denotes |
. |
T13959 |
229-1059 |
sentence |
denotes |
Antibodies used in this study were as follows: rabbit anti-Er81 [14], rabbit anti-Pea3 [14], rabbit anti-PV [14], rabbit anti-eGFP (Molecular Probes, Eugene, Oregon, United States), rabbit anti-Calbindin, rabbit anti-Calretinin (Swant, Bellinzona, Switzerland), rabbit anti-CGRP (Chemicon, Temecula, California, United States), rabbit anti-vGlut1 (Synaptic Systems, Goettingen, Germany), rabbit anti-Brn3a (gift from E. Turner), rabbit anti-TrkA and -p75 (gift from L. F. Reichardt), rabbit anti-Runx3 (Kramer and Arber, unpublished reagent), rabbit anti-Rhodamine (Molecular Probes), mouse anti-neurofilament (American Type Culture Collection, Manassas, Virginia, United States), sheep anti-eGFP (Biogenesis, Poole, United Kingdom), goat anti-LacZ [14], goat anti-TrkC (gift from L. F. Reichardt), and guinea pig anti-Isl1 [14]. |
T13960 |
230-240 |
NNS |
denotes |
Antibodies |
T13961 |
260-264 |
VBD |
denotes |
were |
T13962 |
241-245 |
VBN |
denotes |
used |
T13963 |
246-248 |
IN |
denotes |
in |
T13964 |
249-253 |
DT |
denotes |
this |
T13965 |
254-259 |
NN |
denotes |
study |
T13966 |
265-267 |
IN |
denotes |
as |
T13967 |
268-275 |
VBZ |
denotes |
follows |
T13968 |
275-277 |
: |
denotes |
: |
T13969 |
277-283 |
NN |
denotes |
rabbit |
T13970 |
284-293 |
NN |
denotes |
anti-Er81 |
T13971 |
294-295 |
-LRB- |
denotes |
[ |
T13972 |
295-297 |
CD |
denotes |
14 |
T13973 |
297-298 |
-RRB- |
denotes |
] |
T13974 |
298-300 |
, |
denotes |
, |
T13975 |
300-306 |
NN |
denotes |
rabbit |
T13976 |
307-316 |
JJ |
denotes |
anti-Pea3 |
T13977 |
317-318 |
-LRB- |
denotes |
[ |
T13978 |
318-320 |
CD |
denotes |
14 |
T13979 |
320-321 |
-RRB- |
denotes |
] |
T13980 |
321-323 |
, |
denotes |
, |
T13981 |
323-329 |
NN |
denotes |
rabbit |
T13982 |
330-337 |
JJ |
denotes |
anti-PV |
T13983 |
338-339 |
-LRB- |
denotes |
[ |
T13984 |
339-341 |
CD |
denotes |
14 |
T13985 |
341-342 |
-RRB- |
denotes |
] |
T13986 |
342-344 |
, |
denotes |
, |
T13987 |
344-350 |
NN |
denotes |
rabbit |
T13988 |
351-360 |
JJ |
denotes |
anti-eGFP |
T13989 |
361-362 |
-LRB- |
denotes |
( |
T13990 |
372-378 |
NNP |
denotes |
Probes |
T13991 |
362-371 |
NNP |
denotes |
Molecular |
T13992 |
378-380 |
, |
denotes |
, |
T13993 |
380-386 |
NNP |
denotes |
Eugene |
T13994 |
386-388 |
, |
denotes |
, |
T13995 |
388-394 |
NNP |
denotes |
Oregon |
T13996 |
394-396 |
, |
denotes |
, |
T13997 |
396-402 |
NNP |
denotes |
United |
T13998 |
403-409 |
NNP |
denotes |
States |
T13999 |
409-410 |
-RRB- |
denotes |
) |
T14000 |
410-412 |
, |
denotes |
, |
T14001 |
412-418 |
NN |
denotes |
rabbit |
T14002 |
419-433 |
JJ |
denotes |
anti-Calbindin |
T14003 |
433-435 |
, |
denotes |
, |
T14004 |
435-441 |
NN |
denotes |
rabbit |
T14005 |
442-457 |
JJ |
denotes |
anti-Calretinin |
T14006 |
458-459 |
-LRB- |
denotes |
( |
T14007 |
459-464 |
NNP |
denotes |
Swant |
T14008 |
464-466 |
, |
denotes |
, |
T14009 |
466-476 |
NNP |
denotes |
Bellinzona |
T14010 |
476-478 |
, |
denotes |
, |
T14011 |
478-489 |
NNP |
denotes |
Switzerland |
T14012 |
489-490 |
-RRB- |
denotes |
) |
T14013 |
490-492 |
, |
denotes |
, |
T14014 |
492-498 |
NN |
denotes |
rabbit |
T14015 |
499-508 |
JJ |
denotes |
anti-CGRP |
T14016 |
509-510 |
-LRB- |
denotes |
( |
T14017 |
510-518 |
NNP |
denotes |
Chemicon |
T14018 |
518-520 |
, |
denotes |
, |
T14019 |
520-528 |
NNP |
denotes |
Temecula |
T14020 |
528-530 |
, |
denotes |
, |
T14021 |
530-540 |
NNP |
denotes |
California |
T14022 |
540-542 |
, |
denotes |
, |
T14023 |
542-548 |
NNP |
denotes |
United |
T14024 |
549-555 |
NNP |
denotes |
States |
T14025 |
555-556 |
-RRB- |
denotes |
) |
T14026 |
556-558 |
, |
denotes |
, |
T14027 |
558-564 |
NN |
denotes |
rabbit |
T14028 |
565-576 |
JJ |
denotes |
anti-vGlut1 |
T14029 |
577-578 |
-LRB- |
denotes |
( |
T14030 |
587-594 |
NNP |
denotes |
Systems |
T14031 |
578-586 |
NN |
denotes |
Synaptic |
T14032 |
594-596 |
, |
denotes |
, |
T14033 |
596-606 |
NNP |
denotes |
Goettingen |
T14034 |
606-608 |
, |
denotes |
, |
T14035 |
608-615 |
NNP |
denotes |
Germany |
T14036 |
615-616 |
-RRB- |
denotes |
) |
T14037 |
616-618 |
, |
denotes |
, |
T14038 |
618-624 |
NN |
denotes |
rabbit |
T14039 |
625-635 |
JJ |
denotes |
anti-Brn3a |
T14040 |
636-637 |
-LRB- |
denotes |
( |
T14041 |
637-641 |
NN |
denotes |
gift |
T14042 |
642-646 |
IN |
denotes |
from |
T14043 |
647-649 |
NNP |
denotes |
E. |
T14044 |
650-656 |
NNP |
denotes |
Turner |
T14045 |
656-657 |
-RRB- |
denotes |
) |
T14046 |
657-659 |
, |
denotes |
, |
T14047 |
659-665 |
NN |
denotes |
rabbit |
T14048 |
681-684 |
NN |
denotes |
p75 |
T14049 |
666-675 |
JJ |
denotes |
anti-TrkA |
T14050 |
676-679 |
CC |
denotes |
and |
T14051 |
680-681 |
HYPH |
denotes |
- |
T14052 |
685-686 |
-LRB- |
denotes |
( |
T14053 |
686-690 |
NN |
denotes |
gift |
T14054 |
691-695 |
IN |
denotes |
from |
T14055 |
696-698 |
NNP |
denotes |
L. |
T14056 |
702-711 |
NNP |
denotes |
Reichardt |
T14057 |
699-701 |
NNP |
denotes |
F. |
T14058 |
711-712 |
-RRB- |
denotes |
) |
T14059 |
712-714 |
, |
denotes |
, |
T14060 |
714-720 |
NN |
denotes |
rabbit |
T14061 |
721-731 |
JJ |
denotes |
anti-Runx3 |
T14062 |
732-733 |
-LRB- |
denotes |
( |
T14063 |
733-739 |
NNP |
denotes |
Kramer |
T14064 |
740-743 |
CC |
denotes |
and |
T14065 |
744-749 |
NNP |
denotes |
Arber |
T14066 |
749-751 |
, |
denotes |
, |
T14067 |
751-762 |
JJ |
denotes |
unpublished |
T14068 |
763-770 |
NN |
denotes |
reagent |
T14069 |
770-771 |
-RRB- |
denotes |
) |
T14070 |
771-773 |
, |
denotes |
, |
T14071 |
773-779 |
NN |
denotes |
rabbit |
T14072 |
780-794 |
JJ |
denotes |
anti-Rhodamine |
T14073 |
795-796 |
-LRB- |
denotes |
( |
T14074 |
806-812 |
NNP |
denotes |
Probes |
T14075 |
796-805 |
NNP |
denotes |
Molecular |
T14076 |
812-813 |
-RRB- |
denotes |
) |
T14077 |
813-815 |
, |
denotes |
, |
T14078 |
815-820 |
NN |
denotes |
mouse |
T14079 |
821-839 |
JJ |
denotes |
anti-neurofilament |
T14080 |
840-841 |
-LRB- |
denotes |
( |
T14081 |
863-873 |
NNP |
denotes |
Collection |
T14082 |
841-849 |
NNP |
denotes |
American |
T14083 |
850-854 |
NNP |
denotes |
Type |
T14084 |
855-862 |
NNP |
denotes |
Culture |
T14085 |
873-875 |
, |
denotes |
, |
T14086 |
875-883 |
NNP |
denotes |
Manassas |
T14087 |
883-885 |
, |
denotes |
, |
T14088 |
885-893 |
NNP |
denotes |
Virginia |
T14089 |
893-895 |
, |
denotes |
, |
T14090 |
895-901 |
NNP |
denotes |
United |
T14091 |
902-908 |
NNP |
denotes |
States |
T14092 |
908-909 |
-RRB- |
denotes |
) |
T14093 |
909-911 |
, |
denotes |
, |
T14094 |
911-916 |
NN |
denotes |
sheep |
T14095 |
917-926 |
JJ |
denotes |
anti-eGFP |
T14096 |
927-928 |
-LRB- |
denotes |
( |
T14097 |
928-938 |
NNP |
denotes |
Biogenesis |
T14098 |
938-940 |
, |
denotes |
, |
T14099 |
940-945 |
NNP |
denotes |
Poole |
T14100 |
945-947 |
, |
denotes |
, |
T14101 |
947-953 |
NNP |
denotes |
United |
T14102 |
954-961 |
NNP |
denotes |
Kingdom |
T14103 |
961-962 |
-RRB- |
denotes |
) |
T14104 |
962-964 |
, |
denotes |
, |
T14105 |
964-968 |
NN |
denotes |
goat |
T14106 |
969-978 |
JJ |
denotes |
anti-LacZ |
T14107 |
979-980 |
-LRB- |
denotes |
[ |
T14108 |
980-982 |
CD |
denotes |
14 |
T14109 |
982-983 |
-RRB- |
denotes |
] |
T14110 |
983-985 |
, |
denotes |
, |
T14111 |
985-989 |
NN |
denotes |
goat |
T14112 |
990-999 |
JJ |
denotes |
anti-TrkC |
T14113 |
1000-1001 |
-LRB- |
denotes |
( |
T14114 |
1001-1005 |
NN |
denotes |
gift |
T14115 |
1006-1010 |
IN |
denotes |
from |
T14116 |
1011-1013 |
NNP |
denotes |
L. |
T14117 |
1017-1026 |
NNP |
denotes |
Reichardt |
T14118 |
1014-1016 |
NNP |
denotes |
F. |
T14119 |
1026-1027 |
-RRB- |
denotes |
) |
T14120 |
1027-1029 |
, |
denotes |
, |
T14121 |
1029-1032 |
CC |
denotes |
and |
T14122 |
1033-1039 |
NN |
denotes |
guinea |
T14123 |
1040-1043 |
NN |
denotes |
pig |
T14124 |
1044-1053 |
JJ |
denotes |
anti-Isl1 |
T14125 |
1054-1055 |
-LRB- |
denotes |
[ |
T14126 |
1055-1057 |
CD |
denotes |
14 |
T14127 |
1057-1058 |
-RRB- |
denotes |
] |
T14128 |
1058-1059 |
. |
denotes |
. |
T14129 |
1059-1285 |
sentence |
denotes |
Terminal deoxynucleotidyl transferase-mediated biotinylated UTP nick end labeling (TUNEL) to detect apoptotic cells in E13.5 DRG on cryostat sections was performed as described by the manufacturer (Roche, Basel, Switzerland). |
T14130 |
1060-1068 |
JJ |
denotes |
Terminal |
T14131 |
1086-1097 |
NN |
denotes |
transferase |
T14132 |
1069-1085 |
NN |
denotes |
deoxynucleotidyl |
T14133 |
1098-1106 |
VBN |
denotes |
mediated |
T14134 |
1097-1098 |
HYPH |
denotes |
- |
T14135 |
1133-1141 |
NN |
denotes |
labeling |
T14136 |
1107-1119 |
VBN |
denotes |
biotinylated |
T14137 |
1120-1123 |
NN |
denotes |
UTP |
T14138 |
1124-1128 |
NN |
denotes |
nick |
T14139 |
1129-1132 |
NN |
denotes |
end |
T14140 |
1214-1223 |
VBN |
denotes |
performed |
T14141 |
1142-1143 |
-LRB- |
denotes |
( |
T14142 |
1143-1148 |
NN |
denotes |
TUNEL |
T14143 |
1148-1149 |
-RRB- |
denotes |
) |
T14144 |
1150-1152 |
TO |
denotes |
to |
T14145 |
1153-1159 |
VB |
denotes |
detect |
T14146 |
1160-1169 |
JJ |
denotes |
apoptotic |
T14147 |
1170-1175 |
NNS |
denotes |
cells |
T14148 |
1176-1178 |
IN |
denotes |
in |
T14149 |
1179-1184 |
NN |
denotes |
E13.5 |
T14150 |
1185-1188 |
NN |
denotes |
DRG |
T14151 |
1189-1191 |
IN |
denotes |
on |
T14152 |
1192-1200 |
NN |
denotes |
cryostat |
T14153 |
1201-1209 |
NNS |
denotes |
sections |
T14154 |
1210-1213 |
VBD |
denotes |
was |
T14155 |
1224-1226 |
IN |
denotes |
as |
T14156 |
1227-1236 |
VBN |
denotes |
described |
T14157 |
1237-1239 |
IN |
denotes |
by |
T14158 |
1240-1243 |
DT |
denotes |
the |
T14159 |
1244-1256 |
NN |
denotes |
manufacturer |
T14160 |
1257-1258 |
-LRB- |
denotes |
( |
T14161 |
1258-1263 |
NNP |
denotes |
Roche |
T14162 |
1263-1265 |
, |
denotes |
, |
T14163 |
1265-1270 |
NNP |
denotes |
Basel |
T14164 |
1270-1272 |
, |
denotes |
, |
T14165 |
1272-1283 |
NNP |
denotes |
Switzerland |
T14166 |
1283-1284 |
-RRB- |
denotes |
) |
T14167 |
1284-1285 |
. |
denotes |
. |
T14168 |
1285-1372 |
sentence |
denotes |
Quantitative analysis of TUNEL+ DRG cells was performed essentially as described [27]. |
T14169 |
1286-1298 |
JJ |
denotes |
Quantitative |
T14170 |
1299-1307 |
NN |
denotes |
analysis |
T14171 |
1332-1341 |
VBN |
denotes |
performed |
T14172 |
1308-1310 |
IN |
denotes |
of |
T14173 |
1311-1316 |
NN |
denotes |
TUNEL |
T14174 |
1322-1327 |
NNS |
denotes |
cells |
T14175 |
1316-1317 |
SYM |
denotes |
+ |
T14176 |
1318-1321 |
NN |
denotes |
DRG |
T14177 |
1328-1331 |
VBD |
denotes |
was |
T14178 |
1342-1353 |
RB |
denotes |
essentially |
T14179 |
1357-1366 |
VBN |
denotes |
described |
T14180 |
1354-1356 |
IN |
denotes |
as |
T14181 |
1367-1368 |
-LRB- |
denotes |
[ |
T14182 |
1368-1370 |
CD |
denotes |
27 |
T14183 |
1370-1371 |
-RRB- |
denotes |
] |
T14184 |
1371-1372 |
. |
denotes |
. |
T14185 |
1372-1476 |
sentence |
denotes |
BrdU pulse-chase experiments and LacZ wholemount stainings were performed as previously described [46]. |
T14186 |
1373-1377 |
NN |
denotes |
BrdU |
T14187 |
1390-1401 |
NNS |
denotes |
experiments |
T14188 |
1378-1383 |
NN |
denotes |
pulse |
T14189 |
1384-1389 |
NN |
denotes |
chase |
T14190 |
1383-1384 |
HYPH |
denotes |
- |
T14191 |
1437-1446 |
VBN |
denotes |
performed |
T14192 |
1402-1405 |
CC |
denotes |
and |
T14193 |
1406-1410 |
NN |
denotes |
LacZ |
T14194 |
1422-1431 |
NNS |
denotes |
stainings |
T14195 |
1411-1421 |
NN |
denotes |
wholemount |
T14196 |
1432-1436 |
VBD |
denotes |
were |
T14197 |
1447-1449 |
IN |
denotes |
as |
T14198 |
1461-1470 |
VBN |
denotes |
described |
T14199 |
1450-1460 |
RB |
denotes |
previously |
T14200 |
1471-1472 |
-LRB- |
denotes |
[ |
T14202 |
1474-1475 |
-RRB- |
denotes |
] |
T14203 |
1475-1476 |
. |
denotes |
. |
T14204 |
1476-1748 |
sentence |
denotes |
For anterograde tracing experiments to visualize projections of sensory neurons, rhodamine-conjugated dextran (Molecular Probes) was injected into single lumbar (L3) DRG at E13.5 or applied to whole lumbar dorsal roots (L3) at postnatal day (P) 5 using glass capillaries. |
T14205 |
1477-1480 |
IN |
denotes |
For |
T14206 |
1516-1525 |
VB |
denotes |
visualize |
T14207 |
1481-1492 |
JJ |
denotes |
anterograde |
T14208 |
1501-1512 |
NNS |
denotes |
experiments |
T14209 |
1493-1500 |
NN |
denotes |
tracing |
T14210 |
1513-1515 |
TO |
denotes |
to |
T14211 |
1610-1618 |
VBN |
denotes |
injected |
T14212 |
1526-1537 |
NNS |
denotes |
projections |
T14213 |
1538-1540 |
IN |
denotes |
of |
T14214 |
1541-1548 |
JJ |
denotes |
sensory |
T14215 |
1549-1556 |
NNS |
denotes |
neurons |
T14216 |
1556-1558 |
, |
denotes |
, |
T14217 |
1558-1567 |
NN |
denotes |
rhodamine |
T14218 |
1568-1578 |
VBN |
denotes |
conjugated |
T14219 |
1567-1568 |
HYPH |
denotes |
- |
T14220 |
1579-1586 |
NN |
denotes |
dextran |
T14221 |
1587-1588 |
-LRB- |
denotes |
( |
T14222 |
1598-1604 |
NNP |
denotes |
Probes |
T14223 |
1588-1597 |
NNP |
denotes |
Molecular |
T14224 |
1604-1605 |
-RRB- |
denotes |
) |
T14225 |
1606-1609 |
VBD |
denotes |
was |
T14226 |
1619-1623 |
IN |
denotes |
into |
T14227 |
1624-1630 |
JJ |
denotes |
single |
T14228 |
1643-1646 |
NN |
denotes |
DRG |
T14229 |
1631-1637 |
NN |
denotes |
lumbar |
T14230 |
1638-1639 |
-LRB- |
denotes |
( |
T14231 |
1639-1641 |
NN |
denotes |
L3 |
T14232 |
1641-1642 |
-RRB- |
denotes |
) |
T14233 |
1647-1649 |
IN |
denotes |
at |
T14234 |
1650-1655 |
NN |
denotes |
E13.5 |
T14235 |
1656-1658 |
CC |
denotes |
or |
T14236 |
1659-1666 |
VBN |
denotes |
applied |
T14237 |
1667-1669 |
IN |
denotes |
to |
T14238 |
1670-1675 |
JJ |
denotes |
whole |
T14239 |
1690-1695 |
NNS |
denotes |
roots |
T14240 |
1676-1682 |
JJ |
denotes |
lumbar |
T14241 |
1683-1689 |
JJ |
denotes |
dorsal |
T14242 |
1696-1697 |
-LRB- |
denotes |
( |
T14243 |
1697-1699 |
NN |
denotes |
L3 |
T14244 |
1699-1700 |
-RRB- |
denotes |
) |
T14245 |
1701-1703 |
IN |
denotes |
at |
T14246 |
1704-1713 |
JJ |
denotes |
postnatal |
T14247 |
1714-1717 |
NN |
denotes |
day |
T14248 |
1718-1719 |
-LRB- |
denotes |
( |
T14249 |
1719-1720 |
NN |
denotes |
P |
T14250 |
1720-1721 |
-RRB- |
denotes |
) |
T14251 |
1722-1723 |
CD |
denotes |
5 |
T14252 |
1724-1729 |
VBG |
denotes |
using |
T14253 |
1730-1735 |
NN |
denotes |
glass |
T14254 |
1736-1747 |
NNS |
denotes |
capillaries |
T14255 |
1747-1748 |
. |
denotes |
. |
T14256 |
1748-1825 |
sentence |
denotes |
After injection, animals were incubated for 2–3 h (E13.5) or overnight (P5). |
T14257 |
1749-1754 |
IN |
denotes |
After |
T14258 |
1779-1788 |
VBN |
denotes |
incubated |
T14259 |
1755-1764 |
NN |
denotes |
injection |
T14260 |
1764-1766 |
, |
denotes |
, |
T14261 |
1766-1773 |
NNS |
denotes |
animals |
T14262 |
1774-1778 |
VBD |
denotes |
were |
T14263 |
1789-1792 |
IN |
denotes |
for |
T14264 |
1793-1794 |
CD |
denotes |
2 |
T14265 |
1795-1796 |
CD |
denotes |
3 |
T14266 |
1794-1795 |
SYM |
denotes |
– |
T14267 |
1797-1798 |
NN |
denotes |
h |
T14268 |
1799-1800 |
-LRB- |
denotes |
( |
T14269 |
1800-1805 |
NN |
denotes |
E13.5 |
T14270 |
1805-1806 |
-RRB- |
denotes |
) |
T14271 |
1807-1809 |
CC |
denotes |
or |
T14272 |
1810-1819 |
RB |
denotes |
overnight |
T14273 |
1820-1821 |
-LRB- |
denotes |
( |
T14274 |
1821-1823 |
NN |
denotes |
P5 |
T14275 |
1823-1824 |
-RRB- |
denotes |
) |
T14276 |
1824-1825 |
. |
denotes |
. |
T14277 |
1825-1980 |
sentence |
denotes |
Cryostat sections were processed for immunohistochemistry as described [14] using fluorophore-conjugated secondary antibodies (1:1,000, Molecular Probes). |
T14278 |
1826-1834 |
NN |
denotes |
Cryostat |
T14279 |
1835-1843 |
NNS |
denotes |
sections |
T14280 |
1849-1858 |
VBN |
denotes |
processed |
T14281 |
1844-1848 |
VBD |
denotes |
were |
T14282 |
1859-1862 |
IN |
denotes |
for |
T14283 |
1863-1883 |
NN |
denotes |
immunohistochemistry |
T14284 |
1884-1886 |
IN |
denotes |
as |
T14285 |
1887-1896 |
VBN |
denotes |
described |
T14286 |
1897-1898 |
-LRB- |
denotes |
[ |
T14287 |
1898-1900 |
CD |
denotes |
14 |
T14288 |
1900-1901 |
-RRB- |
denotes |
] |
T14289 |
1902-1907 |
VBG |
denotes |
using |
T14290 |
1908-1919 |
NN |
denotes |
fluorophore |
T14291 |
1920-1930 |
VBN |
denotes |
conjugated |
T14292 |
1919-1920 |
HYPH |
denotes |
- |
T14293 |
1941-1951 |
NNS |
denotes |
antibodies |
T14294 |
1931-1940 |
JJ |
denotes |
secondary |
T14295 |
1952-1953 |
-LRB- |
denotes |
( |
T14296 |
1972-1978 |
NNP |
denotes |
Probes |
T14297 |
1953-1954 |
CD |
denotes |
1 |
T14298 |
1954-1955 |
SYM |
denotes |
: |
T14299 |
1955-1960 |
CD |
denotes |
1,000 |
T14300 |
1960-1962 |
, |
denotes |
, |
T14301 |
1962-1971 |
NNP |
denotes |
Molecular |
T14302 |
1978-1979 |
-RRB- |
denotes |
) |
T14303 |
1979-1980 |
. |
denotes |
. |
T14304 |
1980-2052 |
sentence |
denotes |
Images were collected on an Olympus (Tokyo, Japan) confocal microscope. |
T14305 |
1981-1987 |
NNS |
denotes |
Images |
T14306 |
1993-2002 |
VBN |
denotes |
collected |
T14307 |
1988-1992 |
VBD |
denotes |
were |
T14308 |
2003-2005 |
IN |
denotes |
on |
T14309 |
2006-2008 |
DT |
denotes |
an |
T14310 |
2041-2051 |
NN |
denotes |
microscope |
T14311 |
2009-2016 |
NNP |
denotes |
Olympus |
T14312 |
2017-2018 |
-LRB- |
denotes |
( |
T14313 |
2018-2023 |
NNP |
denotes |
Tokyo |
T14314 |
2023-2025 |
, |
denotes |
, |
T14315 |
2025-2030 |
NNP |
denotes |
Japan |
T14316 |
2030-2031 |
-RRB- |
denotes |
) |
T14317 |
2032-2040 |
JJ |
denotes |
confocal |
T14318 |
2051-2052 |
. |
denotes |
. |
T14319 |
2052-2316 |
sentence |
denotes |
Images from in situ hybridization experiments were collected with an RT-SPOT camera (Diagnostic Instruments, Sterling Heights, Michigan, United States), and Corel (Eden Prairie, Minnesota, United States) Photo Paint 10.0 was used for digital processing of images. |
T14320 |
2053-2059 |
NNS |
denotes |
Images |
T14321 |
2104-2113 |
VBN |
denotes |
collected |
T14322 |
2060-2064 |
IN |
denotes |
from |
T14323 |
2065-2067 |
FW |
denotes |
in |
T14324 |
2068-2072 |
FW |
denotes |
situ |
T14325 |
2087-2098 |
NNS |
denotes |
experiments |
T14326 |
2073-2086 |
NN |
denotes |
hybridization |
T14327 |
2099-2103 |
VBD |
denotes |
were |
T14328 |
2114-2118 |
IN |
denotes |
with |
T14329 |
2119-2121 |
DT |
denotes |
an |
T14330 |
2130-2136 |
NN |
denotes |
camera |
T14331 |
2122-2124 |
NN |
denotes |
RT |
T14332 |
2125-2129 |
NN |
denotes |
SPOT |
T14333 |
2124-2125 |
HYPH |
denotes |
- |
T14334 |
2137-2138 |
-LRB- |
denotes |
( |
T14335 |
2149-2160 |
NNPS |
denotes |
Instruments |
T14336 |
2138-2148 |
JJ |
denotes |
Diagnostic |
T14337 |
2160-2162 |
, |
denotes |
, |
T14338 |
2162-2170 |
NNP |
denotes |
Sterling |
T14339 |
2171-2178 |
NNP |
denotes |
Heights |
T14340 |
2178-2180 |
, |
denotes |
, |
T14341 |
2180-2188 |
NNP |
denotes |
Michigan |
T14342 |
2188-2190 |
, |
denotes |
, |
T14343 |
2190-2196 |
NNP |
denotes |
United |
T14344 |
2197-2203 |
NNP |
denotes |
States |
T14345 |
2203-2204 |
-RRB- |
denotes |
) |
T14346 |
2204-2206 |
, |
denotes |
, |
T14347 |
2206-2209 |
CC |
denotes |
and |
T14348 |
2210-2215 |
NNP |
denotes |
Corel |
T14349 |
2263-2268 |
NNP |
denotes |
Paint |
T14350 |
2216-2217 |
-LRB- |
denotes |
( |
T14351 |
2222-2229 |
NNP |
denotes |
Prairie |
T14352 |
2217-2221 |
NNP |
denotes |
Eden |
T14353 |
2229-2231 |
, |
denotes |
, |
T14354 |
2231-2240 |
NNP |
denotes |
Minnesota |
T14355 |
2240-2242 |
, |
denotes |
, |
T14356 |
2242-2248 |
NNP |
denotes |
United |
T14357 |
2249-2255 |
NNP |
denotes |
States |
T14358 |
2255-2256 |
-RRB- |
denotes |
) |
T14359 |
2257-2262 |
NNP |
denotes |
Photo |
T14360 |
2278-2282 |
VBN |
denotes |
used |
T14361 |
2269-2273 |
CD |
denotes |
10.0 |
T14362 |
2274-2277 |
VBD |
denotes |
was |
T14363 |
2283-2286 |
IN |
denotes |
for |
T14364 |
2287-2294 |
JJ |
denotes |
digital |
T14365 |
2295-2305 |
NN |
denotes |
processing |
T14366 |
2306-2308 |
IN |
denotes |
of |
T14367 |
2309-2315 |
NNS |
denotes |
images |
T14368 |
2315-2316 |
. |
denotes |
. |
T14201 |
1472-1474 |
CD |
denotes |
46 |
R6013 |
T13917 |
T13918 |
advmod |
In,situ |
R6014 |
T13918 |
T13919 |
amod |
situ,hybridization |
R6015 |
T13920 |
T13919 |
cc |
and,hybridization |
R6016 |
T13921 |
T13919 |
conj |
immunohistochemistry,hybridization |
R6017 |
T13923 |
T13924 |
prep |
For,hybridized |
R6018 |
T13925 |
T13926 |
advmod |
in,situ |
R6019 |
T13926 |
T13927 |
amod |
situ,analysis |
R6020 |
T13927 |
T13923 |
pobj |
analysis,For |
R6021 |
T13928 |
T13927 |
compound |
hybridization,analysis |
R6022 |
T13929 |
T13924 |
punct |
", ",hybridized |
R6023 |
T13930 |
T13931 |
compound |
cryostat,sections |
R6024 |
T13931 |
T13924 |
nsubjpass |
sections,hybridized |
R6025 |
T13932 |
T13924 |
auxpass |
were,hybridized |
R6026 |
T13933 |
T13924 |
advcl |
using,hybridized |
R6027 |
T13934 |
T13935 |
npadvmod |
digoxigenin,labeled |
R6028 |
T13935 |
T13937 |
amod |
labeled,probes |
R6029 |
T13936 |
T13935 |
punct |
-,labeled |
R6030 |
T13937 |
T13933 |
dobj |
probes,using |
R6031 |
T13938 |
T13939 |
punct |
[,45 |
R6032 |
T13939 |
T13937 |
parataxis |
45,probes |
R6033 |
T13940 |
T13939 |
punct |
],45 |
R6034 |
T13941 |
T13937 |
acl |
directed,probes |
R6035 |
T13942 |
T13941 |
prep |
against,directed |
R6036 |
T13943 |
T13944 |
compound |
mouse,TrkA |
R6037 |
T13944 |
T13942 |
pobj |
TrkA,against |
R6038 |
T13945 |
T13944 |
cc |
or,TrkA |
R6039 |
T13946 |
T13944 |
conj |
TrkB,TrkA |
R6040 |
T13947 |
T13944 |
punct |
", ",TrkA |
R6041 |
T13948 |
T13944 |
cc |
or,TrkA |
R6042 |
T13949 |
T13950 |
compound |
rat,TrkC |
R6043 |
T13950 |
T13944 |
conj |
TrkC,TrkA |
R6044 |
T13951 |
T13952 |
punct |
(,gift |
R6045 |
T13952 |
T13950 |
parataxis |
gift,TrkC |
R6046 |
T13953 |
T13952 |
prep |
from,gift |
R6047 |
T13954 |
T13955 |
compound |
L.,Parada |
R6048 |
T13955 |
T13953 |
pobj |
Parada,from |
R6049 |
T13956 |
T13955 |
compound |
F.,Parada |
R6050 |
T13957 |
T13952 |
punct |
),gift |
R6051 |
T13958 |
T13924 |
punct |
.,hybridized |
R6052 |
T13960 |
T13961 |
nsubj |
Antibodies,were |
R6053 |
T13962 |
T13960 |
acl |
used,Antibodies |
R6054 |
T13963 |
T13962 |
prep |
in,used |
R6055 |
T13964 |
T13965 |
det |
this,study |
R6056 |
T13965 |
T13963 |
pobj |
study,in |
R6057 |
T13966 |
T13967 |
mark |
as,follows |
R6058 |
T13967 |
T13961 |
advcl |
follows,were |
R6059 |
T13968 |
T13961 |
punct |
: ,were |
R6060 |
T13969 |
T13970 |
compound |
rabbit,anti-Er81 |
R6061 |
T13970 |
T13961 |
dep |
anti-Er81,were |
R6062 |
T13971 |
T13972 |
punct |
[,14 |
R6063 |
T13972 |
T13970 |
parataxis |
14,anti-Er81 |
R6064 |
T13973 |
T13972 |
punct |
],14 |
R6065 |
T13974 |
T13970 |
punct |
", ",anti-Er81 |
R6066 |
T13975 |
T13970 |
conj |
rabbit,anti-Er81 |
R6067 |
T13976 |
T13975 |
amod |
anti-Pea3,rabbit |
R6068 |
T13977 |
T13978 |
punct |
[,14 |
R6069 |
T13978 |
T13975 |
parataxis |
14,rabbit |
R6070 |
T13979 |
T13978 |
punct |
],14 |
R6071 |
T13980 |
T13975 |
punct |
", ",rabbit |
R6072 |
T13981 |
T13975 |
conj |
rabbit,rabbit |
R6073 |
T13982 |
T13981 |
amod |
anti-PV,rabbit |
R6074 |
T13983 |
T13984 |
punct |
[,14 |
R6075 |
T13984 |
T13981 |
parataxis |
14,rabbit |
R6076 |
T13985 |
T13984 |
punct |
],14 |
R6077 |
T13986 |
T13981 |
punct |
", ",rabbit |
R6078 |
T13987 |
T13981 |
conj |
rabbit,rabbit |
R6079 |
T13988 |
T13987 |
amod |
anti-eGFP,rabbit |
R6080 |
T13989 |
T13990 |
punct |
(,Probes |
R6081 |
T13990 |
T13987 |
parataxis |
Probes,rabbit |
R6082 |
T13991 |
T13990 |
compound |
Molecular,Probes |
R6083 |
T13992 |
T13990 |
punct |
", ",Probes |
R6084 |
T13993 |
T13990 |
npadvmod |
Eugene,Probes |
R6085 |
T13994 |
T13990 |
punct |
", ",Probes |
R6086 |
T13995 |
T13990 |
npadvmod |
Oregon,Probes |
R6087 |
T13996 |
T13990 |
punct |
", ",Probes |
R6088 |
T13997 |
T13998 |
compound |
United,States |
R6089 |
T13998 |
T13990 |
npadvmod |
States,Probes |
R6090 |
T13999 |
T13990 |
punct |
),Probes |
R6091 |
T14000 |
T13987 |
punct |
", ",rabbit |
R6092 |
T14001 |
T13987 |
conj |
rabbit,rabbit |
R6093 |
T14002 |
T14001 |
amod |
anti-Calbindin,rabbit |
R6094 |
T14003 |
T14001 |
punct |
", ",rabbit |
R6095 |
T14004 |
T14001 |
conj |
rabbit,rabbit |
R6096 |
T14005 |
T14004 |
amod |
anti-Calretinin,rabbit |
R6097 |
T14006 |
T14007 |
punct |
(,Swant |
R6098 |
T14007 |
T14004 |
parataxis |
Swant,rabbit |
R6099 |
T14008 |
T14007 |
punct |
", ",Swant |
R6100 |
T14009 |
T14007 |
npadvmod |
Bellinzona,Swant |
R6101 |
T14010 |
T14007 |
punct |
", ",Swant |
R6102 |
T14011 |
T14007 |
npadvmod |
Switzerland,Swant |
R6103 |
T14012 |
T14007 |
punct |
),Swant |
R6104 |
T14013 |
T14004 |
punct |
", ",rabbit |
R6105 |
T14014 |
T14004 |
conj |
rabbit,rabbit |
R6106 |
T14015 |
T14014 |
amod |
anti-CGRP,rabbit |
R6107 |
T14016 |
T14017 |
punct |
(,Chemicon |
R6108 |
T14017 |
T14014 |
parataxis |
Chemicon,rabbit |
R6109 |
T14018 |
T14017 |
punct |
", ",Chemicon |
R6110 |
T14019 |
T14017 |
npadvmod |
Temecula,Chemicon |
R6111 |
T14020 |
T14017 |
punct |
", ",Chemicon |
R6112 |
T14021 |
T14017 |
npadvmod |
California,Chemicon |
R6113 |
T14022 |
T14017 |
punct |
", ",Chemicon |
R6114 |
T14023 |
T14024 |
compound |
United,States |
R6115 |
T14024 |
T14017 |
npadvmod |
States,Chemicon |
R6116 |
T14025 |
T14017 |
punct |
),Chemicon |
R6117 |
T14026 |
T14014 |
punct |
", ",rabbit |
R6118 |
T14027 |
T14014 |
conj |
rabbit,rabbit |
R6119 |
T14028 |
T14027 |
amod |
anti-vGlut1,rabbit |
R6120 |
T14029 |
T14030 |
punct |
(,Systems |
R6121 |
T14030 |
T14027 |
parataxis |
Systems,rabbit |
R6122 |
T14031 |
T14030 |
compound |
Synaptic,Systems |
R6123 |
T14032 |
T14030 |
punct |
", ",Systems |
R6124 |
T14033 |
T14030 |
npadvmod |
Goettingen,Systems |
R6125 |
T14034 |
T14030 |
punct |
", ",Systems |
R6126 |
T14035 |
T14030 |
npadvmod |
Germany,Systems |
R6127 |
T14036 |
T14030 |
punct |
),Systems |
R6128 |
T14037 |
T14027 |
punct |
", ",rabbit |
R6129 |
T14038 |
T14027 |
conj |
rabbit,rabbit |
R6130 |
T14039 |
T14038 |
amod |
anti-Brn3a,rabbit |
R6131 |
T14040 |
T14041 |
punct |
(,gift |
R6132 |
T14041 |
T14038 |
parataxis |
gift,rabbit |
R6133 |
T14042 |
T14041 |
prep |
from,gift |
R6134 |
T14043 |
T14044 |
compound |
E.,Turner |
R6135 |
T14044 |
T14042 |
pobj |
Turner,from |
R6136 |
T14045 |
T14041 |
punct |
),gift |
R6137 |
T14046 |
T14038 |
punct |
", ",rabbit |
R6138 |
T14047 |
T14048 |
nmod |
rabbit,p75 |
R6139 |
T14048 |
T14038 |
conj |
p75,rabbit |
R6140 |
T14049 |
T14048 |
amod |
anti-TrkA,p75 |
R6141 |
T14050 |
T14048 |
cc |
and,p75 |
R6142 |
T14051 |
T14048 |
punct |
-,p75 |
R6143 |
T14052 |
T14053 |
punct |
(,gift |
R6144 |
T14053 |
T14048 |
parataxis |
gift,p75 |
R6145 |
T14054 |
T14053 |
prep |
from,gift |
R6146 |
T14055 |
T14056 |
compound |
L.,Reichardt |
R6147 |
T14056 |
T14054 |
pobj |
Reichardt,from |
R6148 |
T14057 |
T14056 |
compound |
F.,Reichardt |
R6149 |
T14058 |
T14053 |
punct |
),gift |
R6150 |
T14059 |
T14048 |
punct |
", ",p75 |
R6151 |
T14060 |
T14048 |
conj |
rabbit,p75 |
R6152 |
T14061 |
T14060 |
amod |
anti-Runx3,rabbit |
R6153 |
T14062 |
T14063 |
punct |
(,Kramer |
R6154 |
T14063 |
T14060 |
parataxis |
Kramer,rabbit |
R6155 |
T14064 |
T14063 |
cc |
and,Kramer |
R6156 |
T14065 |
T14063 |
conj |
Arber,Kramer |
R6157 |
T14066 |
T14063 |
punct |
", ",Kramer |
R6158 |
T14067 |
T14068 |
amod |
unpublished,reagent |
R6159 |
T14068 |
T14063 |
npadvmod |
reagent,Kramer |
R6160 |
T14069 |
T14063 |
punct |
),Kramer |
R6161 |
T14070 |
T14060 |
punct |
", ",rabbit |
R6162 |
T14071 |
T14060 |
conj |
rabbit,rabbit |
R6163 |
T14072 |
T14071 |
amod |
anti-Rhodamine,rabbit |
R6164 |
T14073 |
T14074 |
punct |
(,Probes |
R6165 |
T14074 |
T14071 |
parataxis |
Probes,rabbit |
R6166 |
T14075 |
T14074 |
compound |
Molecular,Probes |
R6167 |
T14076 |
T14074 |
punct |
),Probes |
R6168 |
T14077 |
T14071 |
punct |
", ",rabbit |
R6169 |
T14078 |
T14071 |
conj |
mouse,rabbit |
R6170 |
T14079 |
T14078 |
amod |
anti-neurofilament,mouse |
R6171 |
T14080 |
T14081 |
punct |
(,Collection |
R6172 |
T14081 |
T14078 |
parataxis |
Collection,mouse |
R6173 |
T14082 |
T14081 |
compound |
American,Collection |
R6174 |
T14083 |
T14081 |
compound |
Type,Collection |
R6175 |
T14084 |
T14081 |
compound |
Culture,Collection |
R6176 |
T14085 |
T14081 |
punct |
", ",Collection |
R6177 |
T14086 |
T14081 |
npadvmod |
Manassas,Collection |
R6178 |
T14087 |
T14081 |
punct |
", ",Collection |
R6179 |
T14088 |
T14081 |
npadvmod |
Virginia,Collection |
R6180 |
T14089 |
T14081 |
punct |
", ",Collection |
R6181 |
T14090 |
T14091 |
compound |
United,States |
R6182 |
T14091 |
T14081 |
npadvmod |
States,Collection |
R6183 |
T14092 |
T14081 |
punct |
),Collection |
R6184 |
T14093 |
T14078 |
punct |
", ",mouse |
R6185 |
T14094 |
T14078 |
conj |
sheep,mouse |
R6186 |
T14095 |
T14094 |
amod |
anti-eGFP,sheep |
R6187 |
T14096 |
T14097 |
punct |
(,Biogenesis |
R6188 |
T14097 |
T14094 |
parataxis |
Biogenesis,sheep |
R6189 |
T14098 |
T14097 |
punct |
", ",Biogenesis |
R6190 |
T14099 |
T14097 |
npadvmod |
Poole,Biogenesis |
R6191 |
T14100 |
T14097 |
punct |
", ",Biogenesis |
R6192 |
T14101 |
T14102 |
compound |
United,Kingdom |
R6193 |
T14102 |
T14097 |
npadvmod |
Kingdom,Biogenesis |
R6194 |
T14103 |
T14097 |
punct |
),Biogenesis |
R6195 |
T14104 |
T14094 |
punct |
", ",sheep |
R6196 |
T14105 |
T14094 |
conj |
goat,sheep |
R6197 |
T14106 |
T14105 |
amod |
anti-LacZ,goat |
R6198 |
T14107 |
T14108 |
punct |
[,14 |
R6199 |
T14108 |
T14105 |
parataxis |
14,goat |
R6200 |
T14109 |
T14108 |
punct |
],14 |
R6201 |
T14110 |
T14105 |
punct |
", ",goat |
R6202 |
T14111 |
T14105 |
conj |
goat,goat |
R6203 |
T14112 |
T14111 |
amod |
anti-TrkC,goat |
R6204 |
T14113 |
T14114 |
punct |
(,gift |
R6205 |
T14114 |
T14111 |
parataxis |
gift,goat |
R6206 |
T14115 |
T14114 |
prep |
from,gift |
R6207 |
T14116 |
T14117 |
compound |
L.,Reichardt |
R6208 |
T14117 |
T14115 |
pobj |
Reichardt,from |
R6209 |
T14118 |
T14117 |
compound |
F.,Reichardt |
R6210 |
T14119 |
T14114 |
punct |
),gift |
R6211 |
T14120 |
T14111 |
punct |
", ",goat |
R6212 |
T14121 |
T14111 |
cc |
and,goat |
R6213 |
T14122 |
T14123 |
compound |
guinea,pig |
R6214 |
T14123 |
T14111 |
conj |
pig,goat |
R6215 |
T14124 |
T14123 |
amod |
anti-Isl1,pig |
R6216 |
T14125 |
T14126 |
punct |
[,14 |
R6217 |
T14126 |
T14123 |
parataxis |
14,pig |
R6218 |
T14127 |
T14126 |
punct |
],14 |
R6219 |
T14128 |
T13961 |
punct |
.,were |
R6220 |
T14130 |
T14131 |
amod |
Terminal,transferase |
R6221 |
T14131 |
T14133 |
npadvmod |
transferase,mediated |
R6222 |
T14132 |
T14131 |
compound |
deoxynucleotidyl,transferase |
R6223 |
T14133 |
T14135 |
amod |
mediated,labeling |
R6224 |
T14134 |
T14133 |
punct |
-,mediated |
R6225 |
T14135 |
T14140 |
nsubjpass |
labeling,performed |
R6226 |
T14136 |
T14135 |
amod |
biotinylated,labeling |
R6227 |
T14137 |
T14135 |
compound |
UTP,labeling |
R6228 |
T14138 |
T14135 |
compound |
nick,labeling |
R6229 |
T14139 |
T14135 |
compound |
end,labeling |
R6230 |
T14141 |
T14135 |
punct |
(,labeling |
R6231 |
T14142 |
T14135 |
appos |
TUNEL,labeling |
R6232 |
T14143 |
T14135 |
punct |
),labeling |
R6233 |
T14144 |
T14145 |
aux |
to,detect |
R6234 |
T14145 |
T14135 |
advcl |
detect,labeling |
R6235 |
T14146 |
T14147 |
amod |
apoptotic,cells |
R6236 |
T14147 |
T14145 |
dobj |
cells,detect |
R6237 |
T14148 |
T14145 |
prep |
in,detect |
R6238 |
T14149 |
T14150 |
compound |
E13.5,DRG |
R6239 |
T14150 |
T14148 |
pobj |
DRG,in |
R6240 |
T14151 |
T14145 |
prep |
on,detect |
R6241 |
T14152 |
T14153 |
compound |
cryostat,sections |
R6242 |
T14153 |
T14151 |
pobj |
sections,on |
R6243 |
T14154 |
T14140 |
auxpass |
was,performed |
R6244 |
T14155 |
T14156 |
mark |
as,described |
R6245 |
T14156 |
T14140 |
advcl |
described,performed |
R6246 |
T14157 |
T14156 |
agent |
by,described |
R6247 |
T14158 |
T14159 |
det |
the,manufacturer |
R6248 |
T14159 |
T14157 |
pobj |
manufacturer,by |
R6249 |
T14160 |
T14161 |
punct |
(,Roche |
R6250 |
T14161 |
T14159 |
parataxis |
Roche,manufacturer |
R6251 |
T14162 |
T14161 |
punct |
", ",Roche |
R6252 |
T14163 |
T14161 |
npadvmod |
Basel,Roche |
R6253 |
T14164 |
T14161 |
punct |
", ",Roche |
R6254 |
T14165 |
T14161 |
npadvmod |
Switzerland,Roche |
R6255 |
T14166 |
T14161 |
punct |
),Roche |
R6256 |
T14167 |
T14140 |
punct |
.,performed |
R6257 |
T14169 |
T14170 |
amod |
Quantitative,analysis |
R6258 |
T14170 |
T14171 |
nsubjpass |
analysis,performed |
R6259 |
T14172 |
T14170 |
prep |
of,analysis |
R6260 |
T14173 |
T14174 |
nmod |
TUNEL,cells |
R6261 |
T14174 |
T14172 |
pobj |
cells,of |
R6262 |
T14175 |
T14173 |
punct |
+,TUNEL |
R6263 |
T14176 |
T14174 |
compound |
DRG,cells |
R6264 |
T14177 |
T14171 |
auxpass |
was,performed |
R6265 |
T14178 |
T14179 |
advmod |
essentially,described |
R6266 |
T14179 |
T14171 |
advcl |
described,performed |
R6267 |
T14180 |
T14179 |
mark |
as,described |
R6268 |
T14181 |
T14182 |
punct |
[,27 |
R6269 |
T14182 |
T14179 |
parataxis |
27,described |
R6270 |
T14183 |
T14182 |
punct |
],27 |
R6271 |
T14184 |
T14171 |
punct |
.,performed |
R6272 |
T14186 |
T14187 |
compound |
BrdU,experiments |
R6273 |
T14187 |
T14191 |
nsubjpass |
experiments,performed |
R6274 |
T14188 |
T14189 |
compound |
pulse,chase |
R6275 |
T14189 |
T14187 |
compound |
chase,experiments |
R6276 |
T14190 |
T14189 |
punct |
-,chase |
R6277 |
T14192 |
T14187 |
cc |
and,experiments |
R6278 |
T14193 |
T14194 |
compound |
LacZ,stainings |
R6279 |
T14194 |
T14187 |
conj |
stainings,experiments |
R6280 |
T14195 |
T14194 |
compound |
wholemount,stainings |
R6281 |
T14196 |
T14191 |
auxpass |
were,performed |
R6282 |
T14197 |
T14198 |
mark |
as,described |
R6283 |
T14198 |
T14191 |
advcl |
described,performed |
R6284 |
T14199 |
T14198 |
advmod |
previously,described |
R6285 |
T14200 |
T14201 |
punct |
[,46 |
R6286 |
T14201 |
T14198 |
parataxis |
46,described |
R6287 |
T14202 |
T14201 |
punct |
],46 |
R6288 |
T14203 |
T14191 |
punct |
.,performed |
R6289 |
T14205 |
T14206 |
mark |
For,visualize |
R6290 |
T14206 |
T14211 |
advcl |
visualize,injected |
R6291 |
T14207 |
T14208 |
amod |
anterograde,experiments |
R6292 |
T14208 |
T14206 |
nsubj |
experiments,visualize |
R6293 |
T14209 |
T14208 |
compound |
tracing,experiments |
R6294 |
T14210 |
T14206 |
aux |
to,visualize |
R6295 |
T14212 |
T14206 |
dobj |
projections,visualize |
R6296 |
T14213 |
T14212 |
prep |
of,projections |
R6297 |
T14214 |
T14215 |
amod |
sensory,neurons |
R6298 |
T14215 |
T14213 |
pobj |
neurons,of |
R6299 |
T14216 |
T14211 |
punct |
", ",injected |
R6300 |
T14217 |
T14218 |
npadvmod |
rhodamine,conjugated |
R6301 |
T14218 |
T14220 |
amod |
conjugated,dextran |
R6302 |
T14219 |
T14218 |
punct |
-,conjugated |
R6303 |
T14220 |
T14211 |
nsubjpass |
dextran,injected |
R6304 |
T14221 |
T14222 |
punct |
(,Probes |
R6305 |
T14222 |
T14220 |
parataxis |
Probes,dextran |
R6306 |
T14223 |
T14222 |
compound |
Molecular,Probes |
R6307 |
T14224 |
T14222 |
punct |
),Probes |
R6308 |
T14225 |
T14211 |
auxpass |
was,injected |
R6309 |
T14226 |
T14211 |
prep |
into,injected |
R6310 |
T14227 |
T14228 |
amod |
single,DRG |
R6311 |
T14228 |
T14226 |
pobj |
DRG,into |
R6312 |
T14229 |
T14228 |
nmod |
lumbar,DRG |
R6313 |
T14230 |
T14231 |
punct |
(,L3 |
R6314 |
T14231 |
T14228 |
parataxis |
L3,DRG |
R6315 |
T14232 |
T14231 |
punct |
),L3 |
R6316 |
T14233 |
T14211 |
prep |
at,injected |
R6317 |
T14234 |
T14233 |
pobj |
E13.5,at |
R6318 |
T14235 |
T14211 |
cc |
or,injected |
R6319 |
T14236 |
T14211 |
conj |
applied,injected |
R6320 |
T14237 |
T14236 |
prep |
to,applied |
R6321 |
T14238 |
T14239 |
amod |
whole,roots |
R6322 |
T14239 |
T14237 |
pobj |
roots,to |
R6323 |
T14240 |
T14239 |
amod |
lumbar,roots |
R6324 |
T14241 |
T14239 |
amod |
dorsal,roots |
R6325 |
T14242 |
T14243 |
punct |
(,L3 |
R6326 |
T14243 |
T14239 |
parataxis |
L3,roots |
R6327 |
T14244 |
T14243 |
punct |
),L3 |
R6328 |
T14245 |
T14236 |
prep |
at,applied |
R6329 |
T14246 |
T14247 |
amod |
postnatal,day |
R6330 |
T14247 |
T14245 |
pobj |
day,at |
R6331 |
T14248 |
T14247 |
punct |
(,day |
R6332 |
T14249 |
T14247 |
appos |
P,day |
R6333 |
T14250 |
T14247 |
punct |
),day |
R6334 |
T14251 |
T14247 |
nummod |
5,day |
R6335 |
T14252 |
T14236 |
advcl |
using,applied |
R6336 |
T14253 |
T14254 |
compound |
glass,capillaries |
R6337 |
T14254 |
T14252 |
dobj |
capillaries,using |
R6338 |
T14255 |
T14211 |
punct |
.,injected |
R6339 |
T14257 |
T14258 |
prep |
After,incubated |
R6340 |
T14259 |
T14257 |
pobj |
injection,After |
R6341 |
T14260 |
T14258 |
punct |
", ",incubated |
R6342 |
T14261 |
T14258 |
nsubjpass |
animals,incubated |
R6343 |
T14262 |
T14258 |
auxpass |
were,incubated |
R6344 |
T14263 |
T14258 |
prep |
for,incubated |
R6345 |
T14264 |
T14265 |
quantmod |
2,3 |
R6346 |
T14265 |
T14267 |
nummod |
3,h |
R6347 |
T14266 |
T14265 |
punct |
–,3 |
R6348 |
T14267 |
T14263 |
pobj |
h,for |
R6349 |
T14268 |
T14269 |
punct |
(,E13.5 |
R6350 |
T14269 |
T14267 |
parataxis |
E13.5,h |
R6351 |
T14270 |
T14269 |
punct |
),E13.5 |
R6352 |
T14271 |
T14263 |
cc |
or,for |
R6353 |
T14272 |
T14263 |
conj |
overnight,for |
R6354 |
T14273 |
T14274 |
punct |
(,P5 |
R6355 |
T14274 |
T14272 |
parataxis |
P5,overnight |
R6356 |
T14275 |
T14274 |
punct |
),P5 |
R6357 |
T14276 |
T14258 |
punct |
.,incubated |
R6358 |
T14278 |
T14279 |
compound |
Cryostat,sections |
R6359 |
T14279 |
T14280 |
nsubjpass |
sections,processed |
R6360 |
T14281 |
T14280 |
auxpass |
were,processed |
R6361 |
T14282 |
T14280 |
prep |
for,processed |
R6362 |
T14283 |
T14282 |
pobj |
immunohistochemistry,for |
R6363 |
T14284 |
T14285 |
mark |
as,described |
R6364 |
T14285 |
T14280 |
advcl |
described,processed |
R6365 |
T14286 |
T14287 |
punct |
[,14 |
R6366 |
T14287 |
T14285 |
parataxis |
14,described |
R6367 |
T14288 |
T14287 |
punct |
],14 |
R6368 |
T14289 |
T14280 |
advcl |
using,processed |
R6369 |
T14290 |
T14291 |
npadvmod |
fluorophore,conjugated |
R6370 |
T14291 |
T14293 |
amod |
conjugated,antibodies |
R6371 |
T14292 |
T14291 |
punct |
-,conjugated |
R6372 |
T14293 |
T14289 |
dobj |
antibodies,using |
R6373 |
T14294 |
T14293 |
amod |
secondary,antibodies |
R6374 |
T14295 |
T14296 |
punct |
(,Probes |
R6375 |
T14296 |
T14293 |
parataxis |
Probes,antibodies |
R6376 |
T14297 |
T14296 |
dep |
1,Probes |
R6377 |
T14298 |
T14299 |
punct |
:,"1,000" |
R6378 |
T14299 |
T14297 |
prep |
"1,000",1 |
R6379 |
T14300 |
T14296 |
punct |
", ",Probes |
R6380 |
T14301 |
T14296 |
compound |
Molecular,Probes |
R6381 |
T14302 |
T14296 |
punct |
),Probes |
R6382 |
T14303 |
T14280 |
punct |
.,processed |
R6383 |
T14305 |
T14306 |
nsubjpass |
Images,collected |
R6384 |
T14307 |
T14306 |
auxpass |
were,collected |
R6385 |
T14308 |
T14306 |
prep |
on,collected |
R6386 |
T14309 |
T14310 |
det |
an,microscope |
R6387 |
T14310 |
T14308 |
pobj |
microscope,on |
R6388 |
T14311 |
T14310 |
nmod |
Olympus,microscope |
R6389 |
T14312 |
T14313 |
punct |
(,Tokyo |
R6390 |
T14313 |
T14311 |
parataxis |
Tokyo,Olympus |
R6391 |
T14314 |
T14313 |
punct |
", ",Tokyo |
R6392 |
T14315 |
T14313 |
npadvmod |
Japan,Tokyo |
R6393 |
T14316 |
T14313 |
punct |
),Tokyo |
R6394 |
T14317 |
T14310 |
amod |
confocal,microscope |
R6395 |
T14318 |
T14306 |
punct |
.,collected |
R6396 |
T14320 |
T14321 |
nsubjpass |
Images,collected |
R6397 |
T14322 |
T14320 |
prep |
from,Images |
R6398 |
T14323 |
T14324 |
nmod |
in,situ |
R6399 |
T14324 |
T14325 |
nmod |
situ,experiments |
R6400 |
T14325 |
T14322 |
pobj |
experiments,from |
R6401 |
T14326 |
T14325 |
compound |
hybridization,experiments |
R6402 |
T14327 |
T14321 |
auxpass |
were,collected |
R6403 |
T14328 |
T14321 |
prep |
with,collected |
R6404 |
T14329 |
T14330 |
det |
an,camera |
R6405 |
T14330 |
T14328 |
pobj |
camera,with |
R6406 |
T14331 |
T14332 |
compound |
RT,SPOT |
R6407 |
T14332 |
T14330 |
compound |
SPOT,camera |
R6408 |
T14333 |
T14332 |
punct |
-,SPOT |
R6409 |
T14334 |
T14335 |
punct |
(,Instruments |
R6410 |
T14335 |
T14330 |
parataxis |
Instruments,camera |
R6411 |
T14336 |
T14335 |
amod |
Diagnostic,Instruments |
R6412 |
T14337 |
T14335 |
punct |
", ",Instruments |
R6413 |
T14338 |
T14339 |
compound |
Sterling,Heights |
R6414 |
T14339 |
T14335 |
npadvmod |
Heights,Instruments |
R6415 |
T14340 |
T14335 |
punct |
", ",Instruments |
R6416 |
T14341 |
T14335 |
npadvmod |
Michigan,Instruments |
R6417 |
T14342 |
T14335 |
punct |
", ",Instruments |
R6418 |
T14343 |
T14344 |
compound |
United,States |
R6419 |
T14344 |
T14335 |
npadvmod |
States,Instruments |
R6420 |
T14345 |
T14335 |
punct |
),Instruments |
R6421 |
T14346 |
T14321 |
punct |
", ",collected |
R6422 |
T14347 |
T14321 |
cc |
and,collected |
R6423 |
T14348 |
T14349 |
nmod |
Corel,Paint |
R6424 |
T14349 |
T14360 |
nsubjpass |
Paint,used |
R6425 |
T14350 |
T14351 |
punct |
(,Prairie |
R6426 |
T14351 |
T14348 |
parataxis |
Prairie,Corel |
R6427 |
T14352 |
T14351 |
compound |
Eden,Prairie |
R6428 |
T14353 |
T14351 |
punct |
", ",Prairie |
R6429 |
T14354 |
T14351 |
npadvmod |
Minnesota,Prairie |
R6430 |
T14355 |
T14351 |
punct |
", ",Prairie |
R6431 |
T14356 |
T14357 |
compound |
United,States |
R6432 |
T14357 |
T14351 |
npadvmod |
States,Prairie |
R6433 |
T14358 |
T14351 |
punct |
),Prairie |
R6434 |
T14359 |
T14349 |
compound |
Photo,Paint |
R6435 |
T14360 |
T14321 |
conj |
used,collected |
R6436 |
T14361 |
T14349 |
nummod |
10.0,Paint |
R6437 |
T14362 |
T14360 |
auxpass |
was,used |
R6438 |
T14363 |
T14360 |
prep |
for,used |
R6439 |
T14364 |
T14365 |
amod |
digital,processing |
R6440 |
T14365 |
T14363 |
pobj |
processing,for |
R6441 |
T14366 |
T14365 |
prep |
of,processing |
R6442 |
T14367 |
T14366 |
pobj |
images,of |
R6443 |
T14368 |
T14360 |
punct |
.,used |