Id |
Subject |
Object |
Predicate |
Lexical cue |
T13099 |
0-15 |
JJ |
denotes |
Transcriptional |
T13100 |
16-31 |
NN |
denotes |
transactivation |
T13101 |
32-38 |
NNS |
denotes |
assays |
T13102 |
38-235 |
sentence |
denotes |
The following plasmids were used for transcriptional transactivation assays: pRc/RSV (Invitrogen, Carlsbad, California, United States), pRc/RSV-Er81, pRc/RSV-EWS-Pea3, pTP-5xETS, and pTP-5xETSmut. |
T13103 |
39-42 |
DT |
denotes |
The |
T13104 |
53-61 |
NNS |
denotes |
plasmids |
T13105 |
43-52 |
VBG |
denotes |
following |
T13106 |
67-71 |
VBN |
denotes |
used |
T13107 |
62-66 |
VBD |
denotes |
were |
T13108 |
72-75 |
IN |
denotes |
for |
T13109 |
76-91 |
JJ |
denotes |
transcriptional |
T13110 |
108-114 |
NNS |
denotes |
assays |
T13111 |
92-107 |
NN |
denotes |
transactivation |
T13112 |
114-116 |
: |
denotes |
: |
T13113 |
116-119 |
NN |
denotes |
pRc |
T13114 |
120-123 |
NN |
denotes |
RSV |
T13115 |
119-120 |
HYPH |
denotes |
/ |
T13116 |
124-125 |
-LRB- |
denotes |
( |
T13117 |
125-135 |
NNP |
denotes |
Invitrogen |
T13118 |
135-137 |
, |
denotes |
, |
T13119 |
137-145 |
NNP |
denotes |
Carlsbad |
T13120 |
145-147 |
, |
denotes |
, |
T13121 |
147-157 |
NNP |
denotes |
California |
T13122 |
157-159 |
, |
denotes |
, |
T13123 |
159-165 |
NNP |
denotes |
United |
T13124 |
166-172 |
NNP |
denotes |
States |
T13125 |
172-173 |
-RRB- |
denotes |
) |
T13126 |
173-175 |
, |
denotes |
, |
T13127 |
175-178 |
NN |
denotes |
pRc |
T13128 |
179-182 |
NN |
denotes |
RSV |
T13129 |
178-179 |
HYPH |
denotes |
/ |
T13130 |
183-187 |
NN |
denotes |
Er81 |
T13131 |
182-183 |
HYPH |
denotes |
- |
T13132 |
187-189 |
, |
denotes |
, |
T13133 |
189-192 |
NN |
denotes |
pRc |
T13134 |
193-196 |
NN |
denotes |
RSV |
T13135 |
192-193 |
HYPH |
denotes |
/ |
T13136 |
201-205 |
NN |
denotes |
Pea3 |
T13137 |
196-197 |
HYPH |
denotes |
- |
T13138 |
197-200 |
NN |
denotes |
EWS |
T13139 |
200-201 |
HYPH |
denotes |
- |
T13140 |
205-207 |
, |
denotes |
, |
T13141 |
207-210 |
NN |
denotes |
pTP |
T13142 |
211-216 |
NN |
denotes |
5xETS |
T13143 |
210-211 |
HYPH |
denotes |
- |
T13144 |
216-218 |
, |
denotes |
, |
T13145 |
218-221 |
CC |
denotes |
and |
T13146 |
222-225 |
NN |
denotes |
pTP |
T13147 |
226-234 |
NN |
denotes |
5xETSmut |
T13148 |
225-226 |
HYPH |
denotes |
- |
T13149 |
234-235 |
. |
denotes |
. |
T13150 |
235-370 |
sentence |
denotes |
pRc/RSV-Er81 and pRc/RSV-EWS-Pea3 were obtained by insertion of the cDNAs for Er81 or EWS-Pea3 (gift from J. A. Hassell) into pRc/RSV. |
T13151 |
236-239 |
NN |
denotes |
pRc |
T13152 |
240-243 |
NN |
denotes |
RSV |
T13153 |
239-240 |
HYPH |
denotes |
/ |
T13154 |
244-248 |
NN |
denotes |
Er81 |
T13155 |
243-244 |
HYPH |
denotes |
- |
T13156 |
275-283 |
VBN |
denotes |
obtained |
T13157 |
249-252 |
CC |
denotes |
and |
T13158 |
253-256 |
NN |
denotes |
pRc |
T13159 |
257-260 |
NN |
denotes |
RSV |
T13160 |
256-257 |
HYPH |
denotes |
/ |
T13161 |
265-269 |
NN |
denotes |
Pea3 |
T13162 |
260-261 |
HYPH |
denotes |
- |
T13163 |
261-264 |
NN |
denotes |
EWS |
T13164 |
264-265 |
HYPH |
denotes |
- |
T13165 |
270-274 |
VBD |
denotes |
were |
T13166 |
284-286 |
IN |
denotes |
by |
T13167 |
287-296 |
NN |
denotes |
insertion |
T13168 |
297-299 |
IN |
denotes |
of |
T13169 |
300-303 |
DT |
denotes |
the |
T13170 |
304-309 |
NNS |
denotes |
cDNAs |
T13171 |
310-313 |
IN |
denotes |
for |
T13172 |
314-318 |
NN |
denotes |
Er81 |
T13173 |
319-321 |
CC |
denotes |
or |
T13174 |
322-325 |
NN |
denotes |
EWS |
T13175 |
326-330 |
NN |
denotes |
Pea3 |
T13176 |
325-326 |
HYPH |
denotes |
- |
T13177 |
331-332 |
-LRB- |
denotes |
( |
T13178 |
332-336 |
NN |
denotes |
gift |
T13179 |
337-341 |
IN |
denotes |
from |
T13180 |
342-344 |
NNP |
denotes |
J. |
T13181 |
348-355 |
NNP |
denotes |
Hassell |
T13182 |
345-347 |
NNP |
denotes |
A. |
T13183 |
355-356 |
-RRB- |
denotes |
) |
T13184 |
357-361 |
IN |
denotes |
into |
T13185 |
362-365 |
NN |
denotes |
pRc |
T13186 |
366-369 |
NN |
denotes |
RSV |
T13187 |
365-366 |
HYPH |
denotes |
/ |
T13188 |
369-370 |
. |
denotes |
. |
T13189 |
370-545 |
sentence |
denotes |
pTP-5xETS was constructed by inserting a cassette of five repetitive copies of high-affinity Pea3 binding sites (5′- GCCGGAAGC-3′) [18,19] into a modified version of pTK-Luc. |
T13190 |
371-374 |
NN |
denotes |
pTP |
T13191 |
375-380 |
NN |
denotes |
5xETS |
T13192 |
374-375 |
HYPH |
denotes |
- |
T13193 |
385-396 |
VBN |
denotes |
constructed |
T13194 |
381-384 |
VBD |
denotes |
was |
T13195 |
397-399 |
IN |
denotes |
by |
T13196 |
400-409 |
VBG |
denotes |
inserting |
T13197 |
410-411 |
DT |
denotes |
a |
T13198 |
412-420 |
NN |
denotes |
cassette |
T13199 |
421-423 |
IN |
denotes |
of |
T13200 |
424-428 |
CD |
denotes |
five |
T13201 |
440-446 |
NNS |
denotes |
copies |
T13202 |
429-439 |
JJ |
denotes |
repetitive |
T13203 |
447-449 |
IN |
denotes |
of |
T13204 |
450-454 |
JJ |
denotes |
high |
T13205 |
455-463 |
NN |
denotes |
affinity |
T13206 |
454-455 |
HYPH |
denotes |
- |
T13207 |
477-482 |
NNS |
denotes |
sites |
T13208 |
464-468 |
NN |
denotes |
Pea3 |
T13209 |
469-476 |
NN |
denotes |
binding |
T13210 |
483-484 |
-LRB- |
denotes |
( |
T13211 |
498-499 |
CD |
denotes |
3 |
T13212 |
484-485 |
CD |
denotes |
5 |
T13213 |
485-486 |
SYM |
denotes |
′ |
T13214 |
486-487 |
HYPH |
denotes |
- |
T13215 |
488-497 |
NN |
denotes |
GCCGGAAGC |
T13216 |
497-498 |
HYPH |
denotes |
- |
T13217 |
499-500 |
SYM |
denotes |
′ |
T13218 |
500-501 |
-RRB- |
denotes |
) |
T13219 |
502-503 |
-LRB- |
denotes |
[ |
T13220 |
506-508 |
CD |
denotes |
19 |
T13221 |
503-505 |
CD |
denotes |
18 |
T13222 |
505-506 |
, |
denotes |
, |
T13223 |
508-509 |
-RRB- |
denotes |
] |
T13224 |
510-514 |
IN |
denotes |
into |
T13225 |
515-516 |
DT |
denotes |
a |
T13226 |
526-533 |
NN |
denotes |
version |
T13227 |
517-525 |
VBN |
denotes |
modified |
T13228 |
534-536 |
IN |
denotes |
of |
T13229 |
537-540 |
NN |
denotes |
pTK |
T13230 |
541-544 |
NN |
denotes |
Luc |
T13231 |
540-541 |
HYPH |
denotes |
- |
T13232 |
544-545 |
. |
denotes |
. |
T13233 |
545-662 |
sentence |
denotes |
pTP-5xETSmut was generated as pTP-5xETS but using a mutated complement of the Pea3 binding sites (5′- GCCTATGGC-3′). |
T13234 |
546-549 |
NN |
denotes |
pTP |
T13235 |
550-558 |
NN |
denotes |
5xETSmut |
T13236 |
549-550 |
HYPH |
denotes |
- |
T13237 |
563-572 |
VBN |
denotes |
generated |
T13238 |
559-562 |
VBD |
denotes |
was |
T13239 |
573-575 |
IN |
denotes |
as |
T13240 |
576-579 |
NN |
denotes |
pTP |
T13241 |
580-585 |
NN |
denotes |
5xETS |
T13242 |
579-580 |
HYPH |
denotes |
- |
T13243 |
586-589 |
CC |
denotes |
but |
T13244 |
590-595 |
VBG |
denotes |
using |
T13245 |
596-597 |
DT |
denotes |
a |
T13246 |
606-616 |
NN |
denotes |
complement |
T13247 |
598-605 |
VBN |
denotes |
mutated |
T13248 |
617-619 |
IN |
denotes |
of |
T13249 |
620-623 |
DT |
denotes |
the |
T13250 |
637-642 |
NNS |
denotes |
sites |
T13251 |
624-628 |
NN |
denotes |
Pea3 |
T13252 |
629-636 |
NN |
denotes |
binding |
T13253 |
643-644 |
-LRB- |
denotes |
( |
T13254 |
658-659 |
CD |
denotes |
3 |
T13255 |
644-645 |
CD |
denotes |
5 |
T13256 |
645-646 |
SYM |
denotes |
′ |
T13257 |
646-647 |
HYPH |
denotes |
- |
T13258 |
648-657 |
NN |
denotes |
GCCTATGGC |
T13259 |
657-658 |
HYPH |
denotes |
- |
T13260 |
659-660 |
SYM |
denotes |
′ |
T13261 |
660-661 |
-RRB- |
denotes |
) |
T13262 |
661-662 |
. |
denotes |
. |
T13263 |
662-771 |
sentence |
denotes |
A control plasmid to normalize for transfection efficiency (placZ) and pTK-Luc were a gift from D. Kressler. |
T13264 |
663-664 |
DT |
denotes |
A |
T13265 |
673-680 |
NN |
denotes |
plasmid |
T13266 |
665-672 |
NN |
denotes |
control |
T13267 |
742-746 |
VBD |
denotes |
were |
T13268 |
681-683 |
TO |
denotes |
to |
T13269 |
684-693 |
VB |
denotes |
normalize |
T13270 |
694-697 |
IN |
denotes |
for |
T13271 |
698-710 |
NN |
denotes |
transfection |
T13272 |
711-721 |
NN |
denotes |
efficiency |
T13273 |
722-723 |
-LRB- |
denotes |
( |
T13274 |
723-728 |
NN |
denotes |
placZ |
T13275 |
728-729 |
-RRB- |
denotes |
) |
T13276 |
730-733 |
CC |
denotes |
and |
T13277 |
734-737 |
NN |
denotes |
pTK |
T13278 |
738-741 |
NN |
denotes |
Luc |
T13279 |
737-738 |
HYPH |
denotes |
- |
T13280 |
747-748 |
DT |
denotes |
a |
T13281 |
749-753 |
NN |
denotes |
gift |
T13282 |
754-758 |
IN |
denotes |
from |
T13283 |
759-761 |
NNP |
denotes |
D. |
T13284 |
762-770 |
NNP |
denotes |
Kressler |
T13285 |
770-771 |
. |
denotes |
. |
T13286 |
771-986 |
sentence |
denotes |
COS-7 cells were co-transfected with 1–1.2 μg of total DNA including one of the effector plasmids pRc/RSV-empty, pRc/RSV-Er81, or pRc/RSV-EWS-Pea3; one of the reporter plasmids pTP-5xETS or pTP-5xETSmut; and placZ. |
T13287 |
772-775 |
NN |
denotes |
COS |
T13288 |
778-783 |
NNS |
denotes |
cells |
T13289 |
775-776 |
HYPH |
denotes |
- |
T13290 |
776-777 |
CD |
denotes |
7 |
T13291 |
789-803 |
VBN |
denotes |
co-transfected |
T13292 |
784-788 |
VBD |
denotes |
were |
T13293 |
804-808 |
IN |
denotes |
with |
T13294 |
809-810 |
CD |
denotes |
1 |
T13295 |
811-814 |
CD |
denotes |
1.2 |
T13296 |
810-811 |
SYM |
denotes |
– |
T13297 |
815-817 |
NN |
denotes |
μg |
T13298 |
818-820 |
IN |
denotes |
of |
T13299 |
821-826 |
JJ |
denotes |
total |
T13300 |
827-830 |
NN |
denotes |
DNA |
T13301 |
831-840 |
VBG |
denotes |
including |
T13302 |
841-844 |
CD |
denotes |
one |
T13303 |
845-847 |
IN |
denotes |
of |
T13304 |
848-851 |
DT |
denotes |
the |
T13305 |
861-869 |
NNS |
denotes |
plasmids |
T13306 |
852-860 |
NN |
denotes |
effector |
T13307 |
870-873 |
NN |
denotes |
pRc |
T13308 |
874-877 |
NN |
denotes |
RSV |
T13309 |
873-874 |
HYPH |
denotes |
/ |
T13310 |
877-878 |
HYPH |
denotes |
- |
T13311 |
878-883 |
JJ |
denotes |
empty |
T13312 |
883-885 |
, |
denotes |
, |
T13313 |
885-888 |
NN |
denotes |
pRc |
T13314 |
889-892 |
NN |
denotes |
RSV |
T13315 |
888-889 |
HYPH |
denotes |
/ |
T13316 |
893-897 |
NN |
denotes |
Er81 |
T13317 |
892-893 |
HYPH |
denotes |
- |
T13318 |
897-899 |
, |
denotes |
, |
T13319 |
899-901 |
CC |
denotes |
or |
T13320 |
902-905 |
NN |
denotes |
pRc |
T13321 |
906-909 |
NN |
denotes |
RSV |
T13322 |
905-906 |
HYPH |
denotes |
/ |
T13323 |
914-918 |
NN |
denotes |
Pea3 |
T13324 |
909-910 |
HYPH |
denotes |
- |
T13325 |
910-913 |
NN |
denotes |
EWS |
T13326 |
913-914 |
HYPH |
denotes |
- |
T13327 |
918-919 |
: |
denotes |
; |
T13328 |
920-923 |
CD |
denotes |
one |
T13329 |
924-926 |
IN |
denotes |
of |
T13330 |
927-930 |
DT |
denotes |
the |
T13331 |
940-948 |
NNS |
denotes |
plasmids |
T13332 |
931-939 |
NN |
denotes |
reporter |
T13333 |
949-952 |
NN |
denotes |
pTP |
T13334 |
953-958 |
NN |
denotes |
5xETS |
T13335 |
952-953 |
HYPH |
denotes |
- |
T13336 |
959-961 |
CC |
denotes |
or |
T13337 |
962-965 |
NN |
denotes |
pTP |
T13338 |
966-974 |
NN |
denotes |
5xETSmut |
T13339 |
965-966 |
HYPH |
denotes |
- |
T13340 |
974-975 |
: |
denotes |
; |
T13341 |
976-979 |
CC |
denotes |
and |
T13342 |
980-985 |
NN |
denotes |
placZ |
T13343 |
985-986 |
. |
denotes |
. |
T13344 |
986-1115 |
sentence |
denotes |
Cells were harvested after 25 h and processed for assays to determine luciferase and LacZ activity as described previously [44]. |
T13345 |
987-992 |
NNS |
denotes |
Cells |
T13346 |
998-1007 |
VBN |
denotes |
harvested |
T13347 |
993-997 |
VBD |
denotes |
were |
T13348 |
1008-1013 |
IN |
denotes |
after |
T13349 |
1014-1016 |
CD |
denotes |
25 |
T13350 |
1017-1018 |
NN |
denotes |
h |
T13351 |
1019-1022 |
CC |
denotes |
and |
T13352 |
1023-1032 |
VBN |
denotes |
processed |
T13353 |
1033-1036 |
IN |
denotes |
for |
T13354 |
1037-1043 |
NNS |
denotes |
assays |
T13355 |
1044-1046 |
TO |
denotes |
to |
T13356 |
1047-1056 |
VB |
denotes |
determine |
T13357 |
1057-1067 |
NN |
denotes |
luciferase |
T13358 |
1077-1085 |
NN |
denotes |
activity |
T13359 |
1068-1071 |
CC |
denotes |
and |
T13360 |
1072-1076 |
NN |
denotes |
LacZ |
T13361 |
1086-1088 |
IN |
denotes |
as |
T13362 |
1089-1098 |
VBN |
denotes |
described |
T13363 |
1099-1109 |
RB |
denotes |
previously |
T13364 |
1110-1111 |
-LRB- |
denotes |
[ |
T13365 |
1111-1113 |
CD |
denotes |
44 |
T13366 |
1113-1114 |
-RRB- |
denotes |
] |
T13367 |
1114-1115 |
. |
denotes |
. |
T13368 |
1115-1198 |
sentence |
denotes |
Luciferase values normalized to LacZ activity are referred to as luciferase units. |
T13369 |
1116-1126 |
NN |
denotes |
Luciferase |
T13370 |
1127-1133 |
NNS |
denotes |
values |
T13371 |
1166-1174 |
VBN |
denotes |
referred |
T13372 |
1134-1144 |
VBN |
denotes |
normalized |
T13373 |
1145-1147 |
IN |
denotes |
to |
T13374 |
1148-1152 |
NN |
denotes |
LacZ |
T13375 |
1153-1161 |
NN |
denotes |
activity |
T13376 |
1162-1165 |
VBP |
denotes |
are |
T13377 |
1175-1177 |
IN |
denotes |
to |
T13378 |
1178-1180 |
IN |
denotes |
as |
T13379 |
1181-1191 |
NN |
denotes |
luciferase |
T13380 |
1192-1197 |
NNS |
denotes |
units |
T13381 |
1197-1198 |
. |
denotes |
. |
R5741 |
T13099 |
T13100 |
amod |
Transcriptional,transactivation |
R5742 |
T13100 |
T13101 |
compound |
transactivation,assays |
R5743 |
T13103 |
T13104 |
det |
The,plasmids |
R5744 |
T13104 |
T13106 |
nsubjpass |
plasmids,used |
R5745 |
T13105 |
T13104 |
amod |
following,plasmids |
R5746 |
T13107 |
T13106 |
auxpass |
were,used |
R5747 |
T13108 |
T13106 |
prep |
for,used |
R5748 |
T13109 |
T13110 |
amod |
transcriptional,assays |
R5749 |
T13110 |
T13108 |
pobj |
assays,for |
R5750 |
T13111 |
T13110 |
compound |
transactivation,assays |
R5751 |
T13112 |
T13106 |
punct |
: ,used |
R5752 |
T13113 |
T13114 |
compound |
pRc,RSV |
R5753 |
T13114 |
T13106 |
dobj |
RSV,used |
R5754 |
T13115 |
T13114 |
punct |
/,RSV |
R5755 |
T13116 |
T13117 |
punct |
(,Invitrogen |
R5756 |
T13117 |
T13114 |
parataxis |
Invitrogen,RSV |
R5757 |
T13118 |
T13117 |
punct |
", ",Invitrogen |
R5758 |
T13119 |
T13117 |
npadvmod |
Carlsbad,Invitrogen |
R5759 |
T13120 |
T13117 |
punct |
", ",Invitrogen |
R5760 |
T13121 |
T13117 |
npadvmod |
California,Invitrogen |
R5761 |
T13122 |
T13117 |
punct |
", ",Invitrogen |
R5762 |
T13123 |
T13124 |
compound |
United,States |
R5763 |
T13124 |
T13117 |
npadvmod |
States,Invitrogen |
R5764 |
T13125 |
T13117 |
punct |
),Invitrogen |
R5765 |
T13126 |
T13114 |
punct |
", ",RSV |
R5766 |
T13127 |
T13128 |
compound |
pRc,RSV |
R5767 |
T13128 |
T13130 |
compound |
RSV,Er81 |
R5768 |
T13129 |
T13128 |
punct |
/,RSV |
R5769 |
T13130 |
T13114 |
conj |
Er81,RSV |
R5770 |
T13131 |
T13130 |
punct |
-,Er81 |
R5771 |
T13132 |
T13130 |
punct |
", ",Er81 |
R5772 |
T13133 |
T13134 |
compound |
pRc,RSV |
R5773 |
T13134 |
T13136 |
compound |
RSV,Pea3 |
R5774 |
T13135 |
T13134 |
punct |
/,RSV |
R5775 |
T13136 |
T13130 |
conj |
Pea3,Er81 |
R5776 |
T13137 |
T13136 |
punct |
-,Pea3 |
R5777 |
T13138 |
T13136 |
compound |
EWS,Pea3 |
R5778 |
T13139 |
T13136 |
punct |
-,Pea3 |
R5779 |
T13140 |
T13136 |
punct |
", ",Pea3 |
R5780 |
T13141 |
T13142 |
compound |
pTP,5xETS |
R5781 |
T13142 |
T13136 |
conj |
5xETS,Pea3 |
R5782 |
T13143 |
T13142 |
punct |
-,5xETS |
R5783 |
T13144 |
T13142 |
punct |
", ",5xETS |
R5784 |
T13145 |
T13142 |
cc |
and,5xETS |
R5785 |
T13146 |
T13147 |
compound |
pTP,5xETSmut |
R5786 |
T13147 |
T13142 |
conj |
5xETSmut,5xETS |
R5787 |
T13148 |
T13147 |
punct |
-,5xETSmut |
R5788 |
T13149 |
T13106 |
punct |
.,used |
R5789 |
T13151 |
T13152 |
compound |
pRc,RSV |
R5790 |
T13152 |
T13154 |
compound |
RSV,Er81 |
R5791 |
T13153 |
T13152 |
punct |
/,RSV |
R5792 |
T13154 |
T13156 |
nsubjpass |
Er81,obtained |
R5793 |
T13155 |
T13154 |
punct |
-,Er81 |
R5794 |
T13157 |
T13154 |
cc |
and,Er81 |
R5795 |
T13158 |
T13159 |
compound |
pRc,RSV |
R5796 |
T13159 |
T13161 |
compound |
RSV,Pea3 |
R5797 |
T13160 |
T13159 |
punct |
/,RSV |
R5798 |
T13161 |
T13154 |
conj |
Pea3,Er81 |
R5799 |
T13162 |
T13161 |
punct |
-,Pea3 |
R5800 |
T13163 |
T13161 |
compound |
EWS,Pea3 |
R5801 |
T13164 |
T13161 |
punct |
-,Pea3 |
R5802 |
T13165 |
T13156 |
auxpass |
were,obtained |
R5803 |
T13166 |
T13156 |
prep |
by,obtained |
R5804 |
T13167 |
T13166 |
pobj |
insertion,by |
R5805 |
T13168 |
T13167 |
prep |
of,insertion |
R5806 |
T13169 |
T13170 |
det |
the,cDNAs |
R5807 |
T13170 |
T13168 |
pobj |
cDNAs,of |
R5808 |
T13171 |
T13170 |
prep |
for,cDNAs |
R5809 |
T13172 |
T13171 |
pobj |
Er81,for |
R5810 |
T13173 |
T13172 |
cc |
or,Er81 |
R5811 |
T13174 |
T13175 |
compound |
EWS,Pea3 |
R5812 |
T13175 |
T13172 |
conj |
Pea3,Er81 |
R5813 |
T13176 |
T13175 |
punct |
-,Pea3 |
R5814 |
T13177 |
T13178 |
punct |
(,gift |
R5815 |
T13178 |
T13175 |
parataxis |
gift,Pea3 |
R5816 |
T13179 |
T13178 |
prep |
from,gift |
R5817 |
T13180 |
T13181 |
compound |
J.,Hassell |
R5818 |
T13181 |
T13179 |
pobj |
Hassell,from |
R5819 |
T13182 |
T13181 |
compound |
A.,Hassell |
R5820 |
T13183 |
T13178 |
punct |
),gift |
R5821 |
T13184 |
T13167 |
prep |
into,insertion |
R5822 |
T13185 |
T13186 |
compound |
pRc,RSV |
R5823 |
T13186 |
T13184 |
pobj |
RSV,into |
R5824 |
T13187 |
T13186 |
punct |
/,RSV |
R5825 |
T13188 |
T13156 |
punct |
.,obtained |
R5826 |
T13190 |
T13191 |
compound |
pTP,5xETS |
R5827 |
T13191 |
T13193 |
nsubjpass |
5xETS,constructed |
R5828 |
T13192 |
T13191 |
punct |
-,5xETS |
R5829 |
T13194 |
T13193 |
auxpass |
was,constructed |
R5830 |
T13195 |
T13193 |
prep |
by,constructed |
R5831 |
T13196 |
T13195 |
pcomp |
inserting,by |
R5832 |
T13197 |
T13198 |
det |
a,cassette |
R5833 |
T13198 |
T13196 |
dobj |
cassette,inserting |
R5834 |
T13199 |
T13198 |
prep |
of,cassette |
R5835 |
T13200 |
T13201 |
nummod |
five,copies |
R5836 |
T13201 |
T13199 |
pobj |
copies,of |
R5837 |
T13202 |
T13201 |
amod |
repetitive,copies |
R5838 |
T13203 |
T13201 |
prep |
of,copies |
R5839 |
T13204 |
T13205 |
amod |
high,affinity |
R5840 |
T13205 |
T13207 |
compound |
affinity,sites |
R5841 |
T13206 |
T13205 |
punct |
-,affinity |
R5842 |
T13207 |
T13203 |
pobj |
sites,of |
R5843 |
T13208 |
T13207 |
compound |
Pea3,sites |
R5844 |
T13209 |
T13207 |
compound |
binding,sites |
R5845 |
T13210 |
T13211 |
punct |
(,3 |
R5846 |
T13211 |
T13207 |
parataxis |
3,sites |
R5847 |
T13212 |
T13211 |
dep |
5,3 |
R5848 |
T13213 |
T13212 |
punct |
′,5 |
R5849 |
T13214 |
T13211 |
punct |
-,3 |
R5850 |
T13215 |
T13211 |
dep |
GCCGGAAGC,3 |
R5851 |
T13216 |
T13211 |
punct |
-,3 |
R5852 |
T13217 |
T13211 |
punct |
′,3 |
R5853 |
T13218 |
T13211 |
punct |
),3 |
R5854 |
T13219 |
T13220 |
punct |
[,19 |
R5855 |
T13220 |
T13207 |
parataxis |
19,sites |
R5856 |
T13221 |
T13220 |
nummod |
18,19 |
R5857 |
T13222 |
T13220 |
punct |
",",19 |
R5858 |
T13223 |
T13220 |
punct |
],19 |
R5859 |
T13224 |
T13196 |
prep |
into,inserting |
R5860 |
T13225 |
T13226 |
det |
a,version |
R5861 |
T13226 |
T13224 |
pobj |
version,into |
R5862 |
T13227 |
T13226 |
amod |
modified,version |
R5863 |
T13228 |
T13226 |
prep |
of,version |
R5864 |
T13229 |
T13230 |
compound |
pTK,Luc |
R5865 |
T13230 |
T13228 |
pobj |
Luc,of |
R5866 |
T13231 |
T13230 |
punct |
-,Luc |
R5867 |
T13232 |
T13193 |
punct |
.,constructed |
R5868 |
T13234 |
T13235 |
compound |
pTP,5xETSmut |
R5869 |
T13235 |
T13237 |
nsubjpass |
5xETSmut,generated |
R5870 |
T13236 |
T13235 |
punct |
-,5xETSmut |
R5871 |
T13238 |
T13237 |
auxpass |
was,generated |
R5872 |
T13239 |
T13237 |
prep |
as,generated |
R5873 |
T13240 |
T13241 |
compound |
pTP,5xETS |
R5874 |
T13241 |
T13239 |
pobj |
5xETS,as |
R5875 |
T13242 |
T13241 |
punct |
-,5xETS |
R5876 |
T13243 |
T13239 |
cc |
but,as |
R5877 |
T13244 |
T13239 |
conj |
using,as |
R5878 |
T13245 |
T13246 |
det |
a,complement |
R5879 |
T13246 |
T13244 |
dobj |
complement,using |
R5880 |
T13247 |
T13246 |
amod |
mutated,complement |
R5881 |
T13248 |
T13246 |
prep |
of,complement |
R5882 |
T13249 |
T13250 |
det |
the,sites |
R5883 |
T13250 |
T13248 |
pobj |
sites,of |
R5884 |
T13251 |
T13250 |
compound |
Pea3,sites |
R5885 |
T13252 |
T13250 |
compound |
binding,sites |
R5886 |
T13253 |
T13254 |
punct |
(,3 |
R5887 |
T13254 |
T13246 |
parataxis |
3,complement |
R5888 |
T13255 |
T13254 |
dep |
5,3 |
R5889 |
T13256 |
T13255 |
punct |
′,5 |
R5890 |
T13257 |
T13254 |
punct |
-,3 |
R5891 |
T13258 |
T13254 |
dep |
GCCTATGGC,3 |
R5892 |
T13259 |
T13254 |
punct |
-,3 |
R5893 |
T13260 |
T13254 |
punct |
′,3 |
R5894 |
T13261 |
T13254 |
punct |
),3 |
R5895 |
T13262 |
T13237 |
punct |
.,generated |
R5896 |
T13264 |
T13265 |
det |
A,plasmid |
R5897 |
T13265 |
T13267 |
nsubj |
plasmid,were |
R5898 |
T13266 |
T13265 |
compound |
control,plasmid |
R5899 |
T13268 |
T13269 |
aux |
to,normalize |
R5900 |
T13269 |
T13265 |
advcl |
normalize,plasmid |
R5901 |
T13270 |
T13269 |
prep |
for,normalize |
R5902 |
T13271 |
T13272 |
compound |
transfection,efficiency |
R5903 |
T13272 |
T13270 |
pobj |
efficiency,for |
R5904 |
T13273 |
T13274 |
punct |
(,placZ |
R5905 |
T13274 |
T13272 |
parataxis |
placZ,efficiency |
R5906 |
T13275 |
T13274 |
punct |
),placZ |
R5907 |
T13276 |
T13272 |
cc |
and,efficiency |
R5908 |
T13277 |
T13278 |
compound |
pTK,Luc |
R5909 |
T13278 |
T13272 |
conj |
Luc,efficiency |
R5910 |
T13279 |
T13278 |
punct |
-,Luc |
R5911 |
T13280 |
T13281 |
det |
a,gift |
R5912 |
T13281 |
T13267 |
attr |
gift,were |
R5913 |
T13282 |
T13281 |
prep |
from,gift |
R5914 |
T13283 |
T13284 |
compound |
D.,Kressler |
R5915 |
T13284 |
T13282 |
pobj |
Kressler,from |
R5916 |
T13285 |
T13267 |
punct |
.,were |
R5917 |
T13287 |
T13288 |
nmod |
COS,cells |
R5918 |
T13288 |
T13291 |
nsubjpass |
cells,co-transfected |
R5919 |
T13289 |
T13287 |
punct |
-,COS |
R5920 |
T13290 |
T13287 |
nummod |
7,COS |
R5921 |
T13292 |
T13291 |
auxpass |
were,co-transfected |
R5922 |
T13293 |
T13291 |
prep |
with,co-transfected |
R5923 |
T13294 |
T13295 |
quantmod |
1,1.2 |
R5924 |
T13295 |
T13297 |
nummod |
1.2,μg |
R5925 |
T13296 |
T13295 |
punct |
–,1.2 |
R5926 |
T13297 |
T13293 |
pobj |
μg,with |
R5927 |
T13298 |
T13297 |
prep |
of,μg |
R5928 |
T13299 |
T13300 |
amod |
total,DNA |
R5929 |
T13300 |
T13298 |
pobj |
DNA,of |
R5930 |
T13301 |
T13297 |
prep |
including,μg |
R5931 |
T13302 |
T13301 |
pobj |
one,including |
R5932 |
T13303 |
T13302 |
prep |
of,one |
R5933 |
T13304 |
T13305 |
det |
the,plasmids |
R5934 |
T13305 |
T13303 |
pobj |
plasmids,of |
R5935 |
T13306 |
T13305 |
compound |
effector,plasmids |
R5936 |
T13307 |
T13308 |
compound |
pRc,RSV |
R5937 |
T13308 |
T13302 |
appos |
RSV,one |
R5938 |
T13309 |
T13308 |
punct |
/,RSV |
R5939 |
T13310 |
T13308 |
punct |
-,RSV |
R5940 |
T13311 |
T13308 |
amod |
empty,RSV |
R5941 |
T13312 |
T13308 |
punct |
", ",RSV |
R5942 |
T13313 |
T13314 |
compound |
pRc,RSV |
R5943 |
T13314 |
T13316 |
compound |
RSV,Er81 |
R5944 |
T13315 |
T13314 |
punct |
/,RSV |
R5945 |
T13316 |
T13308 |
conj |
Er81,RSV |
R5946 |
T13317 |
T13316 |
punct |
-,Er81 |
R5947 |
T13318 |
T13316 |
punct |
", ",Er81 |
R5948 |
T13319 |
T13316 |
cc |
or,Er81 |
R5949 |
T13320 |
T13321 |
compound |
pRc,RSV |
R5950 |
T13321 |
T13323 |
compound |
RSV,Pea3 |
R5951 |
T13322 |
T13321 |
punct |
/,RSV |
R5952 |
T13323 |
T13316 |
conj |
Pea3,Er81 |
R5953 |
T13324 |
T13323 |
punct |
-,Pea3 |
R5954 |
T13325 |
T13323 |
compound |
EWS,Pea3 |
R5955 |
T13326 |
T13323 |
punct |
-,Pea3 |
R5956 |
T13327 |
T13302 |
punct |
;,one |
R5957 |
T13328 |
T13302 |
conj |
one,one |
R5958 |
T13329 |
T13328 |
prep |
of,one |
R5959 |
T13330 |
T13331 |
det |
the,plasmids |
R5960 |
T13331 |
T13329 |
pobj |
plasmids,of |
R5961 |
T13332 |
T13331 |
compound |
reporter,plasmids |
R5962 |
T13333 |
T13334 |
compound |
pTP,5xETS |
R5963 |
T13334 |
T13328 |
appos |
5xETS,one |
R5964 |
T13335 |
T13334 |
punct |
-,5xETS |
R5965 |
T13336 |
T13334 |
cc |
or,5xETS |
R5966 |
T13337 |
T13338 |
compound |
pTP,5xETSmut |
R5967 |
T13338 |
T13334 |
conj |
5xETSmut,5xETS |
R5968 |
T13339 |
T13338 |
punct |
-,5xETSmut |
R5969 |
T13340 |
T13328 |
punct |
;,one |
R5970 |
T13341 |
T13328 |
cc |
and,one |
R5971 |
T13342 |
T13328 |
conj |
placZ,one |
R5972 |
T13343 |
T13291 |
punct |
.,co-transfected |
R5973 |
T13345 |
T13346 |
nsubjpass |
Cells,harvested |
R5974 |
T13347 |
T13346 |
auxpass |
were,harvested |
R5975 |
T13348 |
T13346 |
prep |
after,harvested |
R5976 |
T13349 |
T13350 |
nummod |
25,h |
R5977 |
T13350 |
T13348 |
pobj |
h,after |
R5978 |
T13351 |
T13346 |
cc |
and,harvested |
R5979 |
T13352 |
T13346 |
conj |
processed,harvested |
R5980 |
T13353 |
T13352 |
prep |
for,processed |
R5981 |
T13354 |
T13353 |
pobj |
assays,for |
R5982 |
T13355 |
T13356 |
aux |
to,determine |
R5983 |
T13356 |
T13352 |
advcl |
determine,processed |
R5984 |
T13357 |
T13358 |
nmod |
luciferase,activity |
R5985 |
T13358 |
T13356 |
dobj |
activity,determine |
R5986 |
T13359 |
T13357 |
cc |
and,luciferase |
R5987 |
T13360 |
T13357 |
conj |
LacZ,luciferase |
R5988 |
T13361 |
T13362 |
mark |
as,described |
R5989 |
T13362 |
T13356 |
advcl |
described,determine |
R5990 |
T13363 |
T13362 |
advmod |
previously,described |
R5991 |
T13364 |
T13365 |
punct |
[,44 |
R5992 |
T13365 |
T13352 |
parataxis |
44,processed |
R5993 |
T13366 |
T13365 |
punct |
],44 |
R5994 |
T13367 |
T13346 |
punct |
.,harvested |
R5995 |
T13369 |
T13370 |
compound |
Luciferase,values |
R5996 |
T13370 |
T13371 |
nsubjpass |
values,referred |
R5997 |
T13372 |
T13370 |
acl |
normalized,values |
R5998 |
T13373 |
T13372 |
prep |
to,normalized |
R5999 |
T13374 |
T13375 |
compound |
LacZ,activity |
R6000 |
T13375 |
T13373 |
pobj |
activity,to |
R6001 |
T13376 |
T13371 |
auxpass |
are,referred |
R6002 |
T13377 |
T13371 |
prep |
to,referred |
R6003 |
T13378 |
T13371 |
prep |
as,referred |
R6004 |
T13379 |
T13380 |
compound |
luciferase,units |
R6005 |
T13380 |
T13378 |
pobj |
units,as |
R6006 |
T13381 |
T13371 |
punct |
.,referred |