Id |
Subject |
Object |
Predicate |
Lexical cue |
T12345 |
0-10 |
NN |
denotes |
Generation |
T12346 |
11-13 |
IN |
denotes |
of |
T12347 |
14-24 |
JJ |
denotes |
transgenic |
T12348 |
25-29 |
NNS |
denotes |
mice |
T12349 |
30-33 |
CC |
denotes |
and |
T12350 |
34-39 |
NN |
denotes |
mouse |
T12351 |
40-48 |
NN |
denotes |
genetics |
T12352 |
48-169 |
sentence |
denotes |
Er81EWS-Pea3 mice were generated following a strategy similar to that described for the generation of Er81NLZ mice [14]. |
T12353 |
49-56 |
NN |
denotes |
Er81EWS |
T12354 |
57-61 |
NN |
denotes |
Pea3 |
T12355 |
56-57 |
HYPH |
denotes |
- |
T12356 |
62-66 |
NNS |
denotes |
mice |
T12357 |
72-81 |
VBN |
denotes |
generated |
T12358 |
67-71 |
VBD |
denotes |
were |
T12359 |
82-91 |
VBG |
denotes |
following |
T12360 |
92-93 |
DT |
denotes |
a |
T12361 |
94-102 |
NN |
denotes |
strategy |
T12362 |
103-110 |
JJ |
denotes |
similar |
T12363 |
111-113 |
IN |
denotes |
to |
T12364 |
114-118 |
DT |
denotes |
that |
T12365 |
119-128 |
VBN |
denotes |
described |
T12366 |
129-132 |
IN |
denotes |
for |
T12367 |
133-136 |
DT |
denotes |
the |
T12368 |
137-147 |
NN |
denotes |
generation |
T12369 |
148-150 |
IN |
denotes |
of |
T12370 |
151-158 |
NN |
denotes |
Er81NLZ |
T12371 |
159-163 |
NNS |
denotes |
mice |
T12372 |
164-165 |
-LRB- |
denotes |
[ |
T12373 |
165-167 |
CD |
denotes |
14 |
T12374 |
167-168 |
-RRB- |
denotes |
] |
T12375 |
168-169 |
. |
denotes |
. |
T12376 |
169-375 |
sentence |
denotes |
In brief, a targeting vector with a cDNA coding for EWS-Pea3 was inserted in frame with the endogenous start ATG into exon 2 of the Er81 genomic locus and used for homologous recombination in W95 ES cells. |
T12377 |
170-172 |
IN |
denotes |
In |
T12378 |
235-243 |
VBN |
denotes |
inserted |
T12379 |
173-178 |
NN |
denotes |
brief |
T12380 |
178-180 |
, |
denotes |
, |
T12381 |
180-181 |
DT |
denotes |
a |
T12382 |
192-198 |
NN |
denotes |
vector |
T12383 |
182-191 |
NN |
denotes |
targeting |
T12384 |
199-203 |
IN |
denotes |
with |
T12385 |
204-205 |
DT |
denotes |
a |
T12386 |
211-217 |
NN |
denotes |
coding |
T12387 |
206-210 |
NN |
denotes |
cDNA |
T12388 |
218-221 |
IN |
denotes |
for |
T12389 |
222-225 |
NN |
denotes |
EWS |
T12390 |
226-230 |
NN |
denotes |
Pea3 |
T12391 |
225-226 |
HYPH |
denotes |
- |
T12392 |
231-234 |
VBD |
denotes |
was |
T12393 |
244-246 |
IN |
denotes |
in |
T12394 |
247-252 |
NN |
denotes |
frame |
T12395 |
253-257 |
IN |
denotes |
with |
T12396 |
258-261 |
DT |
denotes |
the |
T12397 |
279-282 |
NN |
denotes |
ATG |
T12398 |
262-272 |
JJ |
denotes |
endogenous |
T12399 |
273-278 |
NN |
denotes |
start |
T12400 |
283-287 |
IN |
denotes |
into |
T12401 |
288-292 |
NN |
denotes |
exon |
T12402 |
293-294 |
CD |
denotes |
2 |
T12403 |
295-297 |
IN |
denotes |
of |
T12404 |
298-301 |
DT |
denotes |
the |
T12405 |
315-320 |
NN |
denotes |
locus |
T12406 |
302-306 |
NN |
denotes |
Er81 |
T12407 |
307-314 |
JJ |
denotes |
genomic |
T12408 |
321-324 |
CC |
denotes |
and |
T12409 |
325-329 |
VBN |
denotes |
used |
T12410 |
330-333 |
IN |
denotes |
for |
T12411 |
334-344 |
JJ |
denotes |
homologous |
T12412 |
345-358 |
NN |
denotes |
recombination |
T12413 |
359-361 |
IN |
denotes |
in |
T12414 |
362-365 |
NN |
denotes |
W95 |
T12415 |
369-374 |
NNS |
denotes |
cells |
T12416 |
366-368 |
NN |
denotes |
ES |
T12417 |
374-375 |
. |
denotes |
. |
T12418 |
375-476 |
sentence |
denotes |
EWS-Pea3 represents a fusion gene between the amino terminal of EWS and the ETS domain of Pea3 [20]. |
T12419 |
376-379 |
NN |
denotes |
EWS |
T12420 |
380-384 |
NN |
denotes |
Pea3 |
T12421 |
379-380 |
HYPH |
denotes |
- |
T12422 |
385-395 |
VBZ |
denotes |
represents |
T12423 |
396-397 |
DT |
denotes |
a |
T12424 |
405-409 |
NN |
denotes |
gene |
T12425 |
398-404 |
NN |
denotes |
fusion |
T12426 |
410-417 |
IN |
denotes |
between |
T12427 |
418-421 |
DT |
denotes |
the |
T12428 |
428-436 |
NN |
denotes |
terminal |
T12429 |
422-427 |
NN |
denotes |
amino |
T12430 |
437-439 |
IN |
denotes |
of |
T12431 |
440-443 |
NN |
denotes |
EWS |
T12432 |
444-447 |
CC |
denotes |
and |
T12433 |
448-451 |
DT |
denotes |
the |
T12434 |
456-462 |
NN |
denotes |
domain |
T12435 |
452-455 |
NN |
denotes |
ETS |
T12436 |
463-465 |
IN |
denotes |
of |
T12437 |
466-470 |
NN |
denotes |
Pea3 |
T12438 |
471-472 |
-LRB- |
denotes |
[ |
T12439 |
472-474 |
CD |
denotes |
20 |
T12440 |
474-475 |
-RRB- |
denotes |
] |
T12441 |
475-476 |
. |
denotes |
. |
T12442 |
476-612 |
sentence |
denotes |
The primer pair used to specifically detect the Er81EWS-Pea3 allele was 5′- CAGCCACTGCACCTACAAGAC-3′ and 5′- CTTCCTGCTTGATGTCTCCTTC-3′. |
T12443 |
477-480 |
DT |
denotes |
The |
T12444 |
488-492 |
NN |
denotes |
pair |
T12445 |
481-487 |
NN |
denotes |
primer |
T12446 |
545-548 |
VBD |
denotes |
was |
T12447 |
493-497 |
VBN |
denotes |
used |
T12448 |
498-500 |
TO |
denotes |
to |
T12449 |
514-520 |
VB |
denotes |
detect |
T12450 |
501-513 |
RB |
denotes |
specifically |
T12451 |
521-524 |
DT |
denotes |
the |
T12452 |
538-544 |
NN |
denotes |
allele |
T12453 |
525-532 |
NN |
denotes |
Er81EWS |
T12454 |
533-537 |
NN |
denotes |
Pea3 |
T12455 |
532-533 |
HYPH |
denotes |
- |
T12456 |
549-550 |
CD |
denotes |
5 |
T12457 |
553-574 |
NN |
denotes |
CAGCCACTGCACCTACAAGAC |
T12458 |
550-551 |
SYM |
denotes |
′ |
T12459 |
551-552 |
HYPH |
denotes |
- |
T12460 |
574-575 |
HYPH |
denotes |
- |
T12461 |
575-576 |
CD |
denotes |
3 |
T12462 |
576-577 |
SYM |
denotes |
′ |
T12463 |
578-581 |
CC |
denotes |
and |
T12464 |
582-583 |
CD |
denotes |
5 |
T12465 |
586-608 |
NN |
denotes |
CTTCCTGCTTGATGTCTCCTTC |
T12466 |
583-584 |
SYM |
denotes |
′ |
T12467 |
584-585 |
HYPH |
denotes |
- |
T12468 |
608-609 |
HYPH |
denotes |
- |
T12469 |
609-610 |
CD |
denotes |
3 |
T12470 |
610-611 |
SYM |
denotes |
′ |
T12471 |
611-612 |
. |
denotes |
. |
T12472 |
612-912 |
sentence |
denotes |
For the generation of TaumGFP and TauEWS-Pea3 mice, lox-STOP-lox-mGFP-IRES-NLS-LacZ-pA and lox-STOP-lox-EWS-Pea3-IRES-NLS-LacZ-pA targeting cassettes were integrated into exon 2 of the Tau genomic locus (the endogenous start ATG was removed in the targeting vectors; details available upon request). |
T12473 |
613-616 |
IN |
denotes |
For |
T12474 |
768-778 |
VBN |
denotes |
integrated |
T12475 |
617-620 |
DT |
denotes |
the |
T12476 |
621-631 |
NN |
denotes |
generation |
T12477 |
632-634 |
IN |
denotes |
of |
T12478 |
635-642 |
NN |
denotes |
TaumGFP |
T12479 |
659-663 |
NNS |
denotes |
mice |
T12480 |
643-646 |
CC |
denotes |
and |
T12481 |
647-653 |
NN |
denotes |
TauEWS |
T12482 |
654-658 |
NN |
denotes |
Pea3 |
T12483 |
653-654 |
HYPH |
denotes |
- |
T12484 |
663-665 |
, |
denotes |
, |
T12485 |
665-668 |
NN |
denotes |
lox |
T12486 |
697-699 |
NN |
denotes |
pA |
T12487 |
668-669 |
HYPH |
denotes |
- |
T12488 |
669-673 |
NN |
denotes |
STOP |
T12489 |
673-674 |
HYPH |
denotes |
- |
T12490 |
674-677 |
NN |
denotes |
lox |
T12491 |
677-678 |
HYPH |
denotes |
- |
T12492 |
678-682 |
NN |
denotes |
mGFP |
T12493 |
682-683 |
HYPH |
denotes |
- |
T12494 |
683-687 |
NN |
denotes |
IRES |
T12495 |
687-688 |
HYPH |
denotes |
- |
T12496 |
688-691 |
NN |
denotes |
NLS |
T12497 |
691-692 |
HYPH |
denotes |
- |
T12498 |
692-696 |
NN |
denotes |
LacZ |
T12499 |
696-697 |
HYPH |
denotes |
- |
T12500 |
753-762 |
NNS |
denotes |
cassettes |
T12501 |
700-703 |
CC |
denotes |
and |
T12502 |
704-707 |
NN |
denotes |
lox |
T12503 |
740-742 |
NN |
denotes |
pA |
T12504 |
707-708 |
HYPH |
denotes |
- |
T12505 |
708-712 |
NN |
denotes |
STOP |
T12506 |
712-713 |
HYPH |
denotes |
- |
T12507 |
713-716 |
NN |
denotes |
lox |
T12508 |
716-717 |
HYPH |
denotes |
- |
T12509 |
717-720 |
NN |
denotes |
EWS |
T12510 |
720-721 |
HYPH |
denotes |
- |
T12511 |
721-725 |
NN |
denotes |
Pea3 |
T12512 |
725-726 |
HYPH |
denotes |
- |
T12513 |
726-730 |
NN |
denotes |
IRES |
T12514 |
730-731 |
HYPH |
denotes |
- |
T12515 |
731-734 |
NN |
denotes |
NLS |
T12516 |
734-735 |
HYPH |
denotes |
- |
T12517 |
735-739 |
NN |
denotes |
LacZ |
T12518 |
739-740 |
HYPH |
denotes |
- |
T12519 |
743-752 |
NN |
denotes |
targeting |
T12520 |
763-767 |
VBD |
denotes |
were |
T12521 |
779-783 |
IN |
denotes |
into |
T12522 |
784-788 |
NN |
denotes |
exon |
T12523 |
789-790 |
CD |
denotes |
2 |
T12524 |
791-793 |
IN |
denotes |
of |
T12525 |
794-797 |
DT |
denotes |
the |
T12526 |
810-815 |
NN |
denotes |
locus |
T12527 |
798-801 |
NN |
denotes |
Tau |
T12528 |
802-809 |
JJ |
denotes |
genomic |
T12529 |
816-817 |
-LRB- |
denotes |
( |
T12530 |
846-853 |
VBN |
denotes |
removed |
T12531 |
817-820 |
DT |
denotes |
the |
T12532 |
838-841 |
NN |
denotes |
ATG |
T12533 |
821-831 |
JJ |
denotes |
endogenous |
T12534 |
832-837 |
NN |
denotes |
start |
T12535 |
842-845 |
VBD |
denotes |
was |
T12536 |
854-856 |
IN |
denotes |
in |
T12537 |
857-860 |
DT |
denotes |
the |
T12538 |
871-878 |
NNS |
denotes |
vectors |
T12539 |
861-870 |
NN |
denotes |
targeting |
T12540 |
878-879 |
: |
denotes |
; |
T12541 |
880-887 |
NNS |
denotes |
details |
T12542 |
888-897 |
JJ |
denotes |
available |
T12543 |
898-902 |
IN |
denotes |
upon |
T12544 |
903-910 |
NN |
denotes |
request |
T12545 |
910-911 |
-RRB- |
denotes |
) |
T12546 |
911-912 |
. |
denotes |
. |
T12547 |
912-949 |
sentence |
denotes |
mGFP was provided by P. Caroni [25]. |
T12548 |
913-917 |
NN |
denotes |
mGFP |
T12549 |
922-930 |
VBN |
denotes |
provided |
T12550 |
918-921 |
VBD |
denotes |
was |
T12551 |
931-933 |
IN |
denotes |
by |
T12552 |
934-936 |
NNP |
denotes |
P. |
T12553 |
937-943 |
NNP |
denotes |
Caroni |
T12554 |
944-945 |
-LRB- |
denotes |
[ |
T12555 |
945-947 |
CD |
denotes |
25 |
T12556 |
947-948 |
-RRB- |
denotes |
] |
T12557 |
948-949 |
. |
denotes |
. |
T12558 |
949-1073 |
sentence |
denotes |
ES cell recombinants were screened by Southern blot analysis using the probe in the 5′ region as described previously [41]. |
T12559 |
950-952 |
NN |
denotes |
ES |
T12560 |
958-970 |
NNS |
denotes |
recombinants |
T12561 |
953-957 |
NN |
denotes |
cell |
T12562 |
976-984 |
VBN |
denotes |
screened |
T12563 |
971-975 |
VBD |
denotes |
were |
T12564 |
985-987 |
IN |
denotes |
by |
T12565 |
988-996 |
NNP |
denotes |
Southern |
T12566 |
997-1001 |
NN |
denotes |
blot |
T12567 |
1002-1010 |
NN |
denotes |
analysis |
T12568 |
1011-1016 |
VBG |
denotes |
using |
T12569 |
1017-1020 |
DT |
denotes |
the |
T12570 |
1021-1026 |
NN |
denotes |
probe |
T12571 |
1027-1029 |
IN |
denotes |
in |
T12572 |
1030-1033 |
DT |
denotes |
the |
T12573 |
1037-1043 |
NN |
denotes |
region |
T12574 |
1034-1035 |
CD |
denotes |
5 |
T12575 |
1035-1036 |
SYM |
denotes |
′ |
T12576 |
1044-1046 |
IN |
denotes |
as |
T12577 |
1047-1056 |
VBN |
denotes |
described |
T12578 |
1057-1067 |
RB |
denotes |
previously |
T12579 |
1068-1069 |
-LRB- |
denotes |
[ |
T12580 |
1069-1071 |
CD |
denotes |
41 |
T12581 |
1071-1072 |
-RRB- |
denotes |
] |
T12582 |
1072-1073 |
. |
denotes |
. |
T12583 |
1073-1167 |
sentence |
denotes |
Frequency of recombination in 129/Ola ES cells was approximately 1/3 for both Tau constructs. |
T12584 |
1074-1083 |
NN |
denotes |
Frequency |
T12585 |
1121-1124 |
VBD |
denotes |
was |
T12586 |
1084-1086 |
IN |
denotes |
of |
T12587 |
1087-1100 |
NN |
denotes |
recombination |
T12588 |
1101-1103 |
IN |
denotes |
in |
T12589 |
1104-1107 |
CD |
denotes |
129 |
T12590 |
1108-1111 |
NN |
denotes |
Ola |
T12591 |
1107-1108 |
HYPH |
denotes |
/ |
T12592 |
1115-1120 |
NNS |
denotes |
cells |
T12593 |
1112-1114 |
NN |
denotes |
ES |
T12594 |
1125-1138 |
RB |
denotes |
approximately |
T12595 |
1141-1142 |
CD |
denotes |
3 |
T12596 |
1139-1140 |
CD |
denotes |
1 |
T12597 |
1140-1141 |
SYM |
denotes |
/ |
T12598 |
1143-1146 |
IN |
denotes |
for |
T12599 |
1147-1151 |
DT |
denotes |
both |
T12600 |
1156-1166 |
NNS |
denotes |
constructs |
T12601 |
1152-1155 |
NN |
denotes |
Tau |
T12602 |
1166-1167 |
. |
denotes |
. |
T12603 |
1167-1322 |
sentence |
denotes |
For the generation of PVCre mice, mouse genomic clones were obtained by screening a 129SV/J genomic library (Incyte, Wilmington, Delaware, United States). |
T12604 |
1168-1171 |
IN |
denotes |
For |
T12605 |
1228-1236 |
VBN |
denotes |
obtained |
T12606 |
1172-1175 |
DT |
denotes |
the |
T12607 |
1176-1186 |
NN |
denotes |
generation |
T12608 |
1187-1189 |
IN |
denotes |
of |
T12609 |
1190-1195 |
NN |
denotes |
PVCre |
T12610 |
1196-1200 |
NNS |
denotes |
mice |
T12611 |
1200-1202 |
, |
denotes |
, |
T12612 |
1202-1207 |
NN |
denotes |
mouse |
T12613 |
1216-1222 |
NNS |
denotes |
clones |
T12614 |
1208-1215 |
JJ |
denotes |
genomic |
T12615 |
1223-1227 |
VBD |
denotes |
were |
T12616 |
1237-1239 |
IN |
denotes |
by |
T12617 |
1240-1249 |
VBG |
denotes |
screening |
T12618 |
1250-1251 |
DT |
denotes |
a |
T12619 |
1268-1275 |
NN |
denotes |
library |
T12620 |
1252-1257 |
NN |
denotes |
129SV |
T12621 |
1258-1259 |
NN |
denotes |
J |
T12622 |
1257-1258 |
HYPH |
denotes |
/ |
T12623 |
1260-1267 |
JJ |
denotes |
genomic |
T12624 |
1276-1277 |
-LRB- |
denotes |
( |
T12625 |
1277-1283 |
NNP |
denotes |
Incyte |
T12626 |
1283-1285 |
, |
denotes |
, |
T12627 |
1285-1295 |
NNP |
denotes |
Wilmington |
T12628 |
1295-1297 |
, |
denotes |
, |
T12629 |
1297-1305 |
NNP |
denotes |
Delaware |
T12630 |
1305-1307 |
, |
denotes |
, |
T12631 |
1307-1313 |
NNP |
denotes |
United |
T12632 |
1314-1320 |
NNP |
denotes |
States |
T12633 |
1320-1321 |
-RRB- |
denotes |
) |
T12634 |
1321-1322 |
. |
denotes |
. |
T12635 |
1322-1391 |
sentence |
denotes |
For details on the genomic structure of the mouse PV locus see [42]. |
T12636 |
1323-1326 |
IN |
denotes |
For |
T12637 |
1382-1385 |
VBP |
denotes |
see |
T12638 |
1327-1334 |
NNS |
denotes |
details |
T12639 |
1335-1337 |
IN |
denotes |
on |
T12640 |
1338-1341 |
DT |
denotes |
the |
T12641 |
1350-1359 |
NN |
denotes |
structure |
T12642 |
1342-1349 |
JJ |
denotes |
genomic |
T12643 |
1360-1362 |
IN |
denotes |
of |
T12644 |
1363-1366 |
DT |
denotes |
the |
T12645 |
1376-1381 |
NN |
denotes |
locus |
T12646 |
1367-1372 |
NN |
denotes |
mouse |
T12647 |
1373-1375 |
NN |
denotes |
PV |
T12648 |
1386-1387 |
-LRB- |
denotes |
[ |
T12649 |
1387-1389 |
CD |
denotes |
42 |
T12650 |
1389-1390 |
-RRB- |
denotes |
] |
T12651 |
1390-1391 |
. |
denotes |
. |
T12652 |
1391-1635 |
sentence |
denotes |
An IRES-Cre-pA targeting cassette [33] was integrated into the 3′ UTR of exon 5, and ES cell recombinants were screened with a 5′ probe (oligos, 5′- GAGATGACCCAGCCAGGATGCCTC-3′ and 5′- CTGACCACTCTCGCTCCGGTGTCC-3′; genomic DNA, HindIII digest). |
T12653 |
1392-1394 |
DT |
denotes |
An |
T12654 |
1417-1425 |
NN |
denotes |
cassette |
T12655 |
1395-1399 |
NN |
denotes |
IRES |
T12656 |
1404-1406 |
NN |
denotes |
pA |
T12657 |
1399-1400 |
HYPH |
denotes |
- |
T12658 |
1400-1403 |
NN |
denotes |
Cre |
T12659 |
1403-1404 |
HYPH |
denotes |
- |
T12660 |
1407-1416 |
NN |
denotes |
targeting |
T12661 |
1435-1445 |
VBN |
denotes |
integrated |
T12662 |
1426-1427 |
-LRB- |
denotes |
[ |
T12663 |
1427-1429 |
CD |
denotes |
33 |
T12664 |
1429-1430 |
-RRB- |
denotes |
] |
T12665 |
1431-1434 |
VBD |
denotes |
was |
T12666 |
1446-1450 |
IN |
denotes |
into |
T12667 |
1451-1454 |
DT |
denotes |
the |
T12668 |
1458-1461 |
NN |
denotes |
UTR |
T12669 |
1455-1456 |
CD |
denotes |
3 |
T12670 |
1456-1457 |
SYM |
denotes |
′ |
T12671 |
1462-1464 |
IN |
denotes |
of |
T12672 |
1465-1469 |
NN |
denotes |
exon |
T12673 |
1470-1471 |
CD |
denotes |
5 |
T12674 |
1471-1473 |
, |
denotes |
, |
T12675 |
1473-1476 |
CC |
denotes |
and |
T12676 |
1477-1479 |
NN |
denotes |
ES |
T12677 |
1485-1497 |
NNS |
denotes |
recombinants |
T12678 |
1480-1484 |
NN |
denotes |
cell |
T12679 |
1503-1511 |
VBN |
denotes |
screened |
T12680 |
1498-1502 |
VBD |
denotes |
were |
T12681 |
1512-1516 |
IN |
denotes |
with |
T12682 |
1517-1518 |
DT |
denotes |
a |
T12683 |
1522-1527 |
NN |
denotes |
probe |
T12684 |
1519-1520 |
CD |
denotes |
5 |
T12685 |
1520-1521 |
SYM |
denotes |
′ |
T12686 |
1528-1529 |
-LRB- |
denotes |
( |
T12687 |
1627-1633 |
NN |
denotes |
digest |
T12688 |
1529-1535 |
NNS |
denotes |
oligos |
T12689 |
1535-1537 |
, |
denotes |
, |
T12690 |
1537-1538 |
CD |
denotes |
5 |
T12691 |
1541-1565 |
NN |
denotes |
GAGATGACCCAGCCAGGATGCCTC |
T12692 |
1538-1539 |
SYM |
denotes |
′ |
T12693 |
1539-1540 |
HYPH |
denotes |
- |
T12694 |
1565-1566 |
HYPH |
denotes |
- |
T12695 |
1566-1567 |
CD |
denotes |
3 |
T12696 |
1567-1568 |
SYM |
denotes |
′ |
T12697 |
1569-1572 |
CC |
denotes |
and |
T12698 |
1573-1574 |
CD |
denotes |
5 |
T12699 |
1577-1601 |
NN |
denotes |
CTGACCACTCTCGCTCCGGTGTCC |
T12700 |
1574-1575 |
SYM |
denotes |
′ |
T12701 |
1575-1576 |
HYPH |
denotes |
- |
T12702 |
1601-1602 |
HYPH |
denotes |
- |
T12703 |
1602-1603 |
CD |
denotes |
3 |
T12704 |
1603-1604 |
SYM |
denotes |
′ |
T12705 |
1604-1605 |
: |
denotes |
; |
T12706 |
1606-1613 |
JJ |
denotes |
genomic |
T12707 |
1614-1617 |
NN |
denotes |
DNA |
T12708 |
1617-1619 |
, |
denotes |
, |
T12709 |
1619-1626 |
NN |
denotes |
HindIII |
T12710 |
1633-1634 |
-RRB- |
denotes |
) |
T12711 |
1634-1635 |
. |
denotes |
. |
T12712 |
1635-1710 |
sentence |
denotes |
The frequency of recombination in 129/Ola ES cells was approximately 1/20. |
T12713 |
1636-1639 |
DT |
denotes |
The |
T12714 |
1640-1649 |
NN |
denotes |
frequency |
T12715 |
1687-1690 |
VBD |
denotes |
was |
T12716 |
1650-1652 |
IN |
denotes |
of |
T12717 |
1653-1666 |
NN |
denotes |
recombination |
T12718 |
1667-1669 |
IN |
denotes |
in |
T12719 |
1670-1673 |
CD |
denotes |
129 |
T12720 |
1674-1677 |
NN |
denotes |
Ola |
T12721 |
1673-1674 |
HYPH |
denotes |
/ |
T12722 |
1681-1686 |
NNS |
denotes |
cells |
T12723 |
1678-1680 |
NN |
denotes |
ES |
T12724 |
1691-1704 |
RB |
denotes |
approximately |
T12725 |
1707-1709 |
CD |
denotes |
20 |
T12726 |
1705-1706 |
CD |
denotes |
1 |
T12727 |
1706-1707 |
SYM |
denotes |
/ |
T12728 |
1709-1710 |
. |
denotes |
. |
T12729 |
1710-1842 |
sentence |
denotes |
Recombinant clones were aggregated with morula stage embryos to generate chimeric founder mice that transmitted the mutant alleles. |
T12730 |
1711-1722 |
JJ |
denotes |
Recombinant |
T12731 |
1723-1729 |
NNS |
denotes |
clones |
T12732 |
1735-1745 |
VBN |
denotes |
aggregated |
T12733 |
1730-1734 |
VBD |
denotes |
were |
T12734 |
1746-1750 |
IN |
denotes |
with |
T12735 |
1751-1757 |
NN |
denotes |
morula |
T12736 |
1758-1763 |
NN |
denotes |
stage |
T12737 |
1764-1771 |
NNS |
denotes |
embryos |
T12738 |
1772-1774 |
TO |
denotes |
to |
T12739 |
1775-1783 |
VB |
denotes |
generate |
T12740 |
1784-1792 |
JJ |
denotes |
chimeric |
T12741 |
1801-1805 |
NNS |
denotes |
mice |
T12742 |
1793-1800 |
NN |
denotes |
founder |
T12743 |
1806-1810 |
WDT |
denotes |
that |
T12744 |
1811-1822 |
VBD |
denotes |
transmitted |
T12745 |
1823-1826 |
DT |
denotes |
the |
T12746 |
1834-1841 |
NNS |
denotes |
alleles |
T12747 |
1827-1833 |
NN |
denotes |
mutant |
T12748 |
1841-1842 |
. |
denotes |
. |
T12749 |
1842-1949 |
sentence |
denotes |
In all experiments performed in this study, animals were of mixed genetic background (129/Ola and C57Bl6). |
T12750 |
1843-1845 |
IN |
denotes |
In |
T12751 |
1895-1899 |
VBD |
denotes |
were |
T12752 |
1846-1849 |
DT |
denotes |
all |
T12753 |
1850-1861 |
NNS |
denotes |
experiments |
T12754 |
1862-1871 |
VBN |
denotes |
performed |
T12755 |
1872-1874 |
IN |
denotes |
in |
T12756 |
1875-1879 |
DT |
denotes |
this |
T12757 |
1880-1885 |
NN |
denotes |
study |
T12758 |
1885-1887 |
, |
denotes |
, |
T12759 |
1887-1894 |
NNS |
denotes |
animals |
T12760 |
1900-1902 |
IN |
denotes |
of |
T12761 |
1903-1908 |
JJ |
denotes |
mixed |
T12762 |
1917-1927 |
NN |
denotes |
background |
T12763 |
1909-1916 |
JJ |
denotes |
genetic |
T12764 |
1928-1929 |
-LRB- |
denotes |
( |
T12765 |
1933-1936 |
NN |
denotes |
Ola |
T12766 |
1929-1932 |
CD |
denotes |
129 |
T12767 |
1932-1933 |
HYPH |
denotes |
/ |
T12768 |
1937-1940 |
CC |
denotes |
and |
T12769 |
1941-1947 |
NN |
denotes |
C57Bl6 |
T12770 |
1947-1948 |
-RRB- |
denotes |
) |
T12771 |
1948-1949 |
. |
denotes |
. |
T12772 |
1949-2115 |
sentence |
denotes |
Thy1spGFP transgenic mice were generated in analogy to De Paola et al. [25], and for these experiments a strain of mice with early embryonic expression was selected. |
T12773 |
1950-1959 |
NN |
denotes |
Thy1spGFP |
T12774 |
1971-1975 |
NNS |
denotes |
mice |
T12775 |
1960-1970 |
JJ |
denotes |
transgenic |
T12776 |
1981-1990 |
VBN |
denotes |
generated |
T12777 |
1976-1980 |
VBD |
denotes |
were |
T12778 |
1991-1993 |
IN |
denotes |
in |
T12779 |
1994-2001 |
NN |
denotes |
analogy |
T12780 |
2002-2004 |
IN |
denotes |
to |
T12781 |
2005-2007 |
NNP |
denotes |
De |
T12782 |
2008-2013 |
NNP |
denotes |
Paola |
T12783 |
2014-2016 |
FW |
denotes |
et |
T12784 |
2017-2020 |
FW |
denotes |
al. |
T12785 |
2021-2022 |
-LRB- |
denotes |
[ |
T12786 |
2022-2024 |
CD |
denotes |
25 |
T12787 |
2024-2025 |
-RRB- |
denotes |
] |
T12788 |
2025-2027 |
, |
denotes |
, |
T12789 |
2027-2030 |
CC |
denotes |
and |
T12790 |
2031-2034 |
IN |
denotes |
for |
T12791 |
2106-2114 |
VBN |
denotes |
selected |
T12792 |
2035-2040 |
DT |
denotes |
these |
T12793 |
2041-2052 |
NNS |
denotes |
experiments |
T12794 |
2053-2054 |
DT |
denotes |
a |
T12795 |
2055-2061 |
NN |
denotes |
strain |
T12796 |
2062-2064 |
IN |
denotes |
of |
T12797 |
2065-2069 |
NNS |
denotes |
mice |
T12798 |
2070-2074 |
IN |
denotes |
with |
T12799 |
2075-2080 |
JJ |
denotes |
early |
T12800 |
2091-2101 |
NN |
denotes |
expression |
T12801 |
2081-2090 |
JJ |
denotes |
embryonic |
T12802 |
2102-2105 |
VBD |
denotes |
was |
T12803 |
2114-2115 |
. |
denotes |
. |
T12804 |
2115-2265 |
sentence |
denotes |
Isl1Cre and Hb9Cre mouse strains have been described [33,43] and Bax+/− animals were from Jackson Laboratory (Bar Harbor, Maine, United States) [27]. |
T12805 |
2116-2123 |
NN |
denotes |
Isl1Cre |
T12806 |
2141-2148 |
NNS |
denotes |
strains |
T12807 |
2124-2127 |
CC |
denotes |
and |
T12808 |
2128-2134 |
NN |
denotes |
Hb9Cre |
T12809 |
2135-2140 |
NN |
denotes |
mouse |
T12810 |
2159-2168 |
VBN |
denotes |
described |
T12811 |
2149-2153 |
VBP |
denotes |
have |
T12812 |
2154-2158 |
VBN |
denotes |
been |
T12813 |
2169-2170 |
-LRB- |
denotes |
[ |
T12814 |
2173-2175 |
CD |
denotes |
43 |
T12815 |
2170-2172 |
CD |
denotes |
33 |
T12816 |
2172-2173 |
, |
denotes |
, |
T12817 |
2175-2176 |
-RRB- |
denotes |
] |
T12818 |
2177-2180 |
CC |
denotes |
and |
T12819 |
2181-2184 |
NN |
denotes |
Bax |
T12820 |
2188-2195 |
NNS |
denotes |
animals |
T12821 |
2184-2185 |
SYM |
denotes |
+ |
T12822 |
2185-2186 |
HYPH |
denotes |
/ |
T12823 |
2186-2187 |
SYM |
denotes |
− |
T12824 |
2196-2200 |
VBD |
denotes |
were |
T12825 |
2201-2205 |
IN |
denotes |
from |
T12826 |
2206-2213 |
NNP |
denotes |
Jackson |
T12827 |
2214-2224 |
NNP |
denotes |
Laboratory |
T12828 |
2225-2226 |
-LRB- |
denotes |
( |
T12829 |
2230-2236 |
NNP |
denotes |
Harbor |
T12830 |
2226-2229 |
NNP |
denotes |
Bar |
T12831 |
2236-2238 |
, |
denotes |
, |
T12832 |
2238-2243 |
NNP |
denotes |
Maine |
T12833 |
2243-2245 |
, |
denotes |
, |
T12834 |
2245-2251 |
NNP |
denotes |
United |
T12835 |
2252-2258 |
NNP |
denotes |
States |
T12836 |
2258-2259 |
-RRB- |
denotes |
) |
T12837 |
2260-2261 |
-LRB- |
denotes |
[ |
T12838 |
2261-2263 |
CD |
denotes |
27 |
T12839 |
2263-2264 |
-RRB- |
denotes |
] |
T12840 |
2264-2265 |
. |
denotes |
. |
T12841 |
2265-2400 |
sentence |
denotes |
Timed pregnancies were set up to generate embryos of different developmental stages with all genotypes described throughout the study. |
T12842 |
2266-2271 |
JJ |
denotes |
Timed |
T12843 |
2272-2283 |
NNS |
denotes |
pregnancies |
T12844 |
2289-2292 |
VBN |
denotes |
set |
T12845 |
2284-2288 |
VBD |
denotes |
were |
T12846 |
2293-2295 |
RP |
denotes |
up |
T12847 |
2296-2298 |
TO |
denotes |
to |
T12848 |
2299-2307 |
VB |
denotes |
generate |
T12849 |
2308-2315 |
NNS |
denotes |
embryos |
T12850 |
2316-2318 |
IN |
denotes |
of |
T12851 |
2319-2328 |
JJ |
denotes |
different |
T12852 |
2343-2349 |
NNS |
denotes |
stages |
T12853 |
2329-2342 |
JJ |
denotes |
developmental |
T12854 |
2350-2354 |
IN |
denotes |
with |
T12855 |
2369-2378 |
VBN |
denotes |
described |
T12856 |
2355-2358 |
DT |
denotes |
all |
T12857 |
2359-2368 |
NNS |
denotes |
genotypes |
T12858 |
2379-2389 |
IN |
denotes |
throughout |
T12859 |
2390-2393 |
DT |
denotes |
the |
T12860 |
2394-2399 |
NN |
denotes |
study |
T12861 |
2399-2400 |
. |
denotes |
. |
T13099 |
2402-2417 |
JJ |
denotes |
Transcriptional |
T13100 |
2418-2433 |
NN |
denotes |
transactivation |
T13101 |
2434-2440 |
NNS |
denotes |
assays |
T13102 |
2440-2637 |
sentence |
denotes |
The following plasmids were used for transcriptional transactivation assays: pRc/RSV (Invitrogen, Carlsbad, California, United States), pRc/RSV-Er81, pRc/RSV-EWS-Pea3, pTP-5xETS, and pTP-5xETSmut. |
T13103 |
2441-2444 |
DT |
denotes |
The |
T13104 |
2455-2463 |
NNS |
denotes |
plasmids |
T13105 |
2445-2454 |
VBG |
denotes |
following |
T13106 |
2469-2473 |
VBN |
denotes |
used |
T13107 |
2464-2468 |
VBD |
denotes |
were |
T13108 |
2474-2477 |
IN |
denotes |
for |
T13109 |
2478-2493 |
JJ |
denotes |
transcriptional |
T13110 |
2510-2516 |
NNS |
denotes |
assays |
T13111 |
2494-2509 |
NN |
denotes |
transactivation |
T13112 |
2516-2518 |
: |
denotes |
: |
T13113 |
2518-2521 |
NN |
denotes |
pRc |
T13114 |
2522-2525 |
NN |
denotes |
RSV |
T13115 |
2521-2522 |
HYPH |
denotes |
/ |
T13116 |
2526-2527 |
-LRB- |
denotes |
( |
T13117 |
2527-2537 |
NNP |
denotes |
Invitrogen |
T13118 |
2537-2539 |
, |
denotes |
, |
T13119 |
2539-2547 |
NNP |
denotes |
Carlsbad |
T13120 |
2547-2549 |
, |
denotes |
, |
T13121 |
2549-2559 |
NNP |
denotes |
California |
T13122 |
2559-2561 |
, |
denotes |
, |
T13123 |
2561-2567 |
NNP |
denotes |
United |
T13124 |
2568-2574 |
NNP |
denotes |
States |
T13125 |
2574-2575 |
-RRB- |
denotes |
) |
T13126 |
2575-2577 |
, |
denotes |
, |
T13127 |
2577-2580 |
NN |
denotes |
pRc |
T13128 |
2581-2584 |
NN |
denotes |
RSV |
T13129 |
2580-2581 |
HYPH |
denotes |
/ |
T13130 |
2585-2589 |
NN |
denotes |
Er81 |
T13131 |
2584-2585 |
HYPH |
denotes |
- |
T13132 |
2589-2591 |
, |
denotes |
, |
T13133 |
2591-2594 |
NN |
denotes |
pRc |
T13134 |
2595-2598 |
NN |
denotes |
RSV |
T13135 |
2594-2595 |
HYPH |
denotes |
/ |
T13136 |
2603-2607 |
NN |
denotes |
Pea3 |
T13137 |
2598-2599 |
HYPH |
denotes |
- |
T13138 |
2599-2602 |
NN |
denotes |
EWS |
T13139 |
2602-2603 |
HYPH |
denotes |
- |
T13140 |
2607-2609 |
, |
denotes |
, |
T13141 |
2609-2612 |
NN |
denotes |
pTP |
T13142 |
2613-2618 |
NN |
denotes |
5xETS |
T13143 |
2612-2613 |
HYPH |
denotes |
- |
T13144 |
2618-2620 |
, |
denotes |
, |
T13145 |
2620-2623 |
CC |
denotes |
and |
T13146 |
2624-2627 |
NN |
denotes |
pTP |
T13147 |
2628-2636 |
NN |
denotes |
5xETSmut |
T13148 |
2627-2628 |
HYPH |
denotes |
- |
T13149 |
2636-2637 |
. |
denotes |
. |
T13150 |
2637-2772 |
sentence |
denotes |
pRc/RSV-Er81 and pRc/RSV-EWS-Pea3 were obtained by insertion of the cDNAs for Er81 or EWS-Pea3 (gift from J. A. Hassell) into pRc/RSV. |
T13151 |
2638-2641 |
NN |
denotes |
pRc |
T13152 |
2642-2645 |
NN |
denotes |
RSV |
T13153 |
2641-2642 |
HYPH |
denotes |
/ |
T13154 |
2646-2650 |
NN |
denotes |
Er81 |
T13155 |
2645-2646 |
HYPH |
denotes |
- |
T13156 |
2677-2685 |
VBN |
denotes |
obtained |
T13157 |
2651-2654 |
CC |
denotes |
and |
T13158 |
2655-2658 |
NN |
denotes |
pRc |
T13159 |
2659-2662 |
NN |
denotes |
RSV |
T13160 |
2658-2659 |
HYPH |
denotes |
/ |
T13161 |
2667-2671 |
NN |
denotes |
Pea3 |
T13162 |
2662-2663 |
HYPH |
denotes |
- |
T13163 |
2663-2666 |
NN |
denotes |
EWS |
T13164 |
2666-2667 |
HYPH |
denotes |
- |
T13165 |
2672-2676 |
VBD |
denotes |
were |
T13166 |
2686-2688 |
IN |
denotes |
by |
T13167 |
2689-2698 |
NN |
denotes |
insertion |
T13168 |
2699-2701 |
IN |
denotes |
of |
T13169 |
2702-2705 |
DT |
denotes |
the |
T13170 |
2706-2711 |
NNS |
denotes |
cDNAs |
T13171 |
2712-2715 |
IN |
denotes |
for |
T13172 |
2716-2720 |
NN |
denotes |
Er81 |
T13173 |
2721-2723 |
CC |
denotes |
or |
T13174 |
2724-2727 |
NN |
denotes |
EWS |
T13175 |
2728-2732 |
NN |
denotes |
Pea3 |
T13176 |
2727-2728 |
HYPH |
denotes |
- |
T13177 |
2733-2734 |
-LRB- |
denotes |
( |
T13178 |
2734-2738 |
NN |
denotes |
gift |
T13179 |
2739-2743 |
IN |
denotes |
from |
T13180 |
2744-2746 |
NNP |
denotes |
J. |
T13181 |
2750-2757 |
NNP |
denotes |
Hassell |
T13182 |
2747-2749 |
NNP |
denotes |
A. |
T13183 |
2757-2758 |
-RRB- |
denotes |
) |
T13184 |
2759-2763 |
IN |
denotes |
into |
T13185 |
2764-2767 |
NN |
denotes |
pRc |
T13186 |
2768-2771 |
NN |
denotes |
RSV |
T13187 |
2767-2768 |
HYPH |
denotes |
/ |
T13188 |
2771-2772 |
. |
denotes |
. |
T13189 |
2772-2947 |
sentence |
denotes |
pTP-5xETS was constructed by inserting a cassette of five repetitive copies of high-affinity Pea3 binding sites (5′- GCCGGAAGC-3′) [18,19] into a modified version of pTK-Luc. |
T13190 |
2773-2776 |
NN |
denotes |
pTP |
T13191 |
2777-2782 |
NN |
denotes |
5xETS |
T13192 |
2776-2777 |
HYPH |
denotes |
- |
T13193 |
2787-2798 |
VBN |
denotes |
constructed |
T13194 |
2783-2786 |
VBD |
denotes |
was |
T13195 |
2799-2801 |
IN |
denotes |
by |
T13196 |
2802-2811 |
VBG |
denotes |
inserting |
T13197 |
2812-2813 |
DT |
denotes |
a |
T13198 |
2814-2822 |
NN |
denotes |
cassette |
T13199 |
2823-2825 |
IN |
denotes |
of |
T13200 |
2826-2830 |
CD |
denotes |
five |
T13201 |
2842-2848 |
NNS |
denotes |
copies |
T13202 |
2831-2841 |
JJ |
denotes |
repetitive |
T13203 |
2849-2851 |
IN |
denotes |
of |
T13204 |
2852-2856 |
JJ |
denotes |
high |
T13205 |
2857-2865 |
NN |
denotes |
affinity |
T13206 |
2856-2857 |
HYPH |
denotes |
- |
T13207 |
2879-2884 |
NNS |
denotes |
sites |
T13208 |
2866-2870 |
NN |
denotes |
Pea3 |
T13209 |
2871-2878 |
NN |
denotes |
binding |
T13210 |
2885-2886 |
-LRB- |
denotes |
( |
T13211 |
2900-2901 |
CD |
denotes |
3 |
T13212 |
2886-2887 |
CD |
denotes |
5 |
T13213 |
2887-2888 |
SYM |
denotes |
′ |
T13214 |
2888-2889 |
HYPH |
denotes |
- |
T13215 |
2890-2899 |
NN |
denotes |
GCCGGAAGC |
T13216 |
2899-2900 |
HYPH |
denotes |
- |
T13217 |
2901-2902 |
SYM |
denotes |
′ |
T13218 |
2902-2903 |
-RRB- |
denotes |
) |
T13219 |
2904-2905 |
-LRB- |
denotes |
[ |
T13220 |
2908-2910 |
CD |
denotes |
19 |
T13221 |
2905-2907 |
CD |
denotes |
18 |
T13222 |
2907-2908 |
, |
denotes |
, |
T13223 |
2910-2911 |
-RRB- |
denotes |
] |
T13224 |
2912-2916 |
IN |
denotes |
into |
T13225 |
2917-2918 |
DT |
denotes |
a |
T13226 |
2928-2935 |
NN |
denotes |
version |
T13227 |
2919-2927 |
VBN |
denotes |
modified |
T13228 |
2936-2938 |
IN |
denotes |
of |
T13229 |
2939-2942 |
NN |
denotes |
pTK |
T13230 |
2943-2946 |
NN |
denotes |
Luc |
T13231 |
2942-2943 |
HYPH |
denotes |
- |
T13232 |
2946-2947 |
. |
denotes |
. |
T13233 |
2947-3064 |
sentence |
denotes |
pTP-5xETSmut was generated as pTP-5xETS but using a mutated complement of the Pea3 binding sites (5′- GCCTATGGC-3′). |
T13234 |
2948-2951 |
NN |
denotes |
pTP |
T13235 |
2952-2960 |
NN |
denotes |
5xETSmut |
T13236 |
2951-2952 |
HYPH |
denotes |
- |
T13237 |
2965-2974 |
VBN |
denotes |
generated |
T13238 |
2961-2964 |
VBD |
denotes |
was |
T13239 |
2975-2977 |
IN |
denotes |
as |
T13240 |
2978-2981 |
NN |
denotes |
pTP |
T13241 |
2982-2987 |
NN |
denotes |
5xETS |
T13242 |
2981-2982 |
HYPH |
denotes |
- |
T13243 |
2988-2991 |
CC |
denotes |
but |
T13244 |
2992-2997 |
VBG |
denotes |
using |
T13245 |
2998-2999 |
DT |
denotes |
a |
T13246 |
3008-3018 |
NN |
denotes |
complement |
T13247 |
3000-3007 |
VBN |
denotes |
mutated |
T13248 |
3019-3021 |
IN |
denotes |
of |
T13249 |
3022-3025 |
DT |
denotes |
the |
T13250 |
3039-3044 |
NNS |
denotes |
sites |
T13251 |
3026-3030 |
NN |
denotes |
Pea3 |
T13252 |
3031-3038 |
NN |
denotes |
binding |
T13253 |
3045-3046 |
-LRB- |
denotes |
( |
T13254 |
3060-3061 |
CD |
denotes |
3 |
T13255 |
3046-3047 |
CD |
denotes |
5 |
T13256 |
3047-3048 |
SYM |
denotes |
′ |
T13257 |
3048-3049 |
HYPH |
denotes |
- |
T13258 |
3050-3059 |
NN |
denotes |
GCCTATGGC |
T13259 |
3059-3060 |
HYPH |
denotes |
- |
T13260 |
3061-3062 |
SYM |
denotes |
′ |
T13261 |
3062-3063 |
-RRB- |
denotes |
) |
T13262 |
3063-3064 |
. |
denotes |
. |
T13263 |
3064-3173 |
sentence |
denotes |
A control plasmid to normalize for transfection efficiency (placZ) and pTK-Luc were a gift from D. Kressler. |
T13264 |
3065-3066 |
DT |
denotes |
A |
T13265 |
3075-3082 |
NN |
denotes |
plasmid |
T13266 |
3067-3074 |
NN |
denotes |
control |
T13267 |
3144-3148 |
VBD |
denotes |
were |
T13268 |
3083-3085 |
TO |
denotes |
to |
T13269 |
3086-3095 |
VB |
denotes |
normalize |
T13270 |
3096-3099 |
IN |
denotes |
for |
T13271 |
3100-3112 |
NN |
denotes |
transfection |
T13272 |
3113-3123 |
NN |
denotes |
efficiency |
T13273 |
3124-3125 |
-LRB- |
denotes |
( |
T13274 |
3125-3130 |
NN |
denotes |
placZ |
T13275 |
3130-3131 |
-RRB- |
denotes |
) |
T13276 |
3132-3135 |
CC |
denotes |
and |
T13277 |
3136-3139 |
NN |
denotes |
pTK |
T13278 |
3140-3143 |
NN |
denotes |
Luc |
T13279 |
3139-3140 |
HYPH |
denotes |
- |
T13280 |
3149-3150 |
DT |
denotes |
a |
T13281 |
3151-3155 |
NN |
denotes |
gift |
T13282 |
3156-3160 |
IN |
denotes |
from |
T13283 |
3161-3163 |
NNP |
denotes |
D. |
T13284 |
3164-3172 |
NNP |
denotes |
Kressler |
T13285 |
3172-3173 |
. |
denotes |
. |
T13286 |
3173-3388 |
sentence |
denotes |
COS-7 cells were co-transfected with 1–1.2 μg of total DNA including one of the effector plasmids pRc/RSV-empty, pRc/RSV-Er81, or pRc/RSV-EWS-Pea3; one of the reporter plasmids pTP-5xETS or pTP-5xETSmut; and placZ. |
T13287 |
3174-3177 |
NN |
denotes |
COS |
T13288 |
3180-3185 |
NNS |
denotes |
cells |
T13289 |
3177-3178 |
HYPH |
denotes |
- |
T13290 |
3178-3179 |
CD |
denotes |
7 |
T13291 |
3191-3205 |
VBN |
denotes |
co-transfected |
T13292 |
3186-3190 |
VBD |
denotes |
were |
T13293 |
3206-3210 |
IN |
denotes |
with |
T13294 |
3211-3212 |
CD |
denotes |
1 |
T13295 |
3213-3216 |
CD |
denotes |
1.2 |
T13296 |
3212-3213 |
SYM |
denotes |
– |
T13297 |
3217-3219 |
NN |
denotes |
μg |
T13298 |
3220-3222 |
IN |
denotes |
of |
T13299 |
3223-3228 |
JJ |
denotes |
total |
T13300 |
3229-3232 |
NN |
denotes |
DNA |
T13301 |
3233-3242 |
VBG |
denotes |
including |
T13302 |
3243-3246 |
CD |
denotes |
one |
T13303 |
3247-3249 |
IN |
denotes |
of |
T13304 |
3250-3253 |
DT |
denotes |
the |
T13305 |
3263-3271 |
NNS |
denotes |
plasmids |
T13306 |
3254-3262 |
NN |
denotes |
effector |
T13307 |
3272-3275 |
NN |
denotes |
pRc |
T13308 |
3276-3279 |
NN |
denotes |
RSV |
T13309 |
3275-3276 |
HYPH |
denotes |
/ |
T13310 |
3279-3280 |
HYPH |
denotes |
- |
T13311 |
3280-3285 |
JJ |
denotes |
empty |
T13312 |
3285-3287 |
, |
denotes |
, |
T13313 |
3287-3290 |
NN |
denotes |
pRc |
T13314 |
3291-3294 |
NN |
denotes |
RSV |
T13315 |
3290-3291 |
HYPH |
denotes |
/ |
T13316 |
3295-3299 |
NN |
denotes |
Er81 |
T13317 |
3294-3295 |
HYPH |
denotes |
- |
T13318 |
3299-3301 |
, |
denotes |
, |
T13319 |
3301-3303 |
CC |
denotes |
or |
T13320 |
3304-3307 |
NN |
denotes |
pRc |
T13321 |
3308-3311 |
NN |
denotes |
RSV |
T13322 |
3307-3308 |
HYPH |
denotes |
/ |
T13323 |
3316-3320 |
NN |
denotes |
Pea3 |
T13324 |
3311-3312 |
HYPH |
denotes |
- |
T13325 |
3312-3315 |
NN |
denotes |
EWS |
T13326 |
3315-3316 |
HYPH |
denotes |
- |
T13327 |
3320-3321 |
: |
denotes |
; |
T13328 |
3322-3325 |
CD |
denotes |
one |
T13329 |
3326-3328 |
IN |
denotes |
of |
T13330 |
3329-3332 |
DT |
denotes |
the |
T13331 |
3342-3350 |
NNS |
denotes |
plasmids |
T13332 |
3333-3341 |
NN |
denotes |
reporter |
T13333 |
3351-3354 |
NN |
denotes |
pTP |
T13334 |
3355-3360 |
NN |
denotes |
5xETS |
T13335 |
3354-3355 |
HYPH |
denotes |
- |
T13336 |
3361-3363 |
CC |
denotes |
or |
T13337 |
3364-3367 |
NN |
denotes |
pTP |
T13338 |
3368-3376 |
NN |
denotes |
5xETSmut |
T13339 |
3367-3368 |
HYPH |
denotes |
- |
T13340 |
3376-3377 |
: |
denotes |
; |
T13341 |
3378-3381 |
CC |
denotes |
and |
T13342 |
3382-3387 |
NN |
denotes |
placZ |
T13343 |
3387-3388 |
. |
denotes |
. |
T13344 |
3388-3517 |
sentence |
denotes |
Cells were harvested after 25 h and processed for assays to determine luciferase and LacZ activity as described previously [44]. |
T13345 |
3389-3394 |
NNS |
denotes |
Cells |
T13346 |
3400-3409 |
VBN |
denotes |
harvested |
T13347 |
3395-3399 |
VBD |
denotes |
were |
T13348 |
3410-3415 |
IN |
denotes |
after |
T13349 |
3416-3418 |
CD |
denotes |
25 |
T13350 |
3419-3420 |
NN |
denotes |
h |
T13351 |
3421-3424 |
CC |
denotes |
and |
T13352 |
3425-3434 |
VBN |
denotes |
processed |
T13353 |
3435-3438 |
IN |
denotes |
for |
T13354 |
3439-3445 |
NNS |
denotes |
assays |
T13355 |
3446-3448 |
TO |
denotes |
to |
T13356 |
3449-3458 |
VB |
denotes |
determine |
T13357 |
3459-3469 |
NN |
denotes |
luciferase |
T13358 |
3479-3487 |
NN |
denotes |
activity |
T13359 |
3470-3473 |
CC |
denotes |
and |
T13360 |
3474-3478 |
NN |
denotes |
LacZ |
T13361 |
3488-3490 |
IN |
denotes |
as |
T13362 |
3491-3500 |
VBN |
denotes |
described |
T13363 |
3501-3511 |
RB |
denotes |
previously |
T13364 |
3512-3513 |
-LRB- |
denotes |
[ |
T13365 |
3513-3515 |
CD |
denotes |
44 |
T13366 |
3515-3516 |
-RRB- |
denotes |
] |
T13367 |
3516-3517 |
. |
denotes |
. |
T13368 |
3517-3600 |
sentence |
denotes |
Luciferase values normalized to LacZ activity are referred to as luciferase units. |
T13369 |
3518-3528 |
NN |
denotes |
Luciferase |
T13370 |
3529-3535 |
NNS |
denotes |
values |
T13371 |
3568-3576 |
VBN |
denotes |
referred |
T13372 |
3536-3546 |
VBN |
denotes |
normalized |
T13373 |
3547-3549 |
IN |
denotes |
to |
T13374 |
3550-3554 |
NN |
denotes |
LacZ |
T13375 |
3555-3563 |
NN |
denotes |
activity |
T13376 |
3564-3567 |
VBP |
denotes |
are |
T13377 |
3577-3579 |
IN |
denotes |
to |
T13378 |
3580-3582 |
IN |
denotes |
as |
T13379 |
3583-3593 |
NN |
denotes |
luciferase |
T13380 |
3594-3599 |
NNS |
denotes |
units |
T13381 |
3599-3600 |
. |
denotes |
. |
T13917 |
3602-3604 |
FW |
denotes |
In |
T13918 |
3605-3609 |
FW |
denotes |
situ |
T13919 |
3610-3623 |
NN |
denotes |
hybridization |
T13920 |
3624-3627 |
CC |
denotes |
and |
T13921 |
3628-3648 |
NN |
denotes |
immunohistochemistry |
T13922 |
3648-3831 |
sentence |
denotes |
For in situ hybridization analysis, cryostat sections were hybridized using digoxigenin-labeled probes [45] directed against mouse TrkA or TrkB, or rat TrkC (gift from L. F. Parada). |
T13923 |
3649-3652 |
IN |
denotes |
For |
T13924 |
3708-3718 |
VBN |
denotes |
hybridized |
T13925 |
3653-3655 |
FW |
denotes |
in |
T13926 |
3656-3660 |
FW |
denotes |
situ |
T13927 |
3675-3683 |
NN |
denotes |
analysis |
T13928 |
3661-3674 |
NN |
denotes |
hybridization |
T13929 |
3683-3685 |
, |
denotes |
, |
T13930 |
3685-3693 |
NN |
denotes |
cryostat |
T13931 |
3694-3702 |
NNS |
denotes |
sections |
T13932 |
3703-3707 |
VBD |
denotes |
were |
T13933 |
3719-3724 |
VBG |
denotes |
using |
T13934 |
3725-3736 |
NN |
denotes |
digoxigenin |
T13935 |
3737-3744 |
VBN |
denotes |
labeled |
T13936 |
3736-3737 |
HYPH |
denotes |
- |
T13937 |
3745-3751 |
NNS |
denotes |
probes |
T13938 |
3752-3753 |
-LRB- |
denotes |
[ |
T13939 |
3753-3755 |
CD |
denotes |
45 |
T13940 |
3755-3756 |
-RRB- |
denotes |
] |
T13941 |
3757-3765 |
VBN |
denotes |
directed |
T13942 |
3766-3773 |
IN |
denotes |
against |
T13943 |
3774-3779 |
NN |
denotes |
mouse |
T13944 |
3780-3784 |
NN |
denotes |
TrkA |
T13945 |
3785-3787 |
CC |
denotes |
or |
T13946 |
3788-3792 |
NN |
denotes |
TrkB |
T13947 |
3792-3794 |
, |
denotes |
, |
T13948 |
3794-3796 |
CC |
denotes |
or |
T13949 |
3797-3800 |
NN |
denotes |
rat |
T13950 |
3801-3805 |
NN |
denotes |
TrkC |
T13951 |
3806-3807 |
-LRB- |
denotes |
( |
T13952 |
3807-3811 |
NN |
denotes |
gift |
T13953 |
3812-3816 |
IN |
denotes |
from |
T13954 |
3817-3819 |
NNP |
denotes |
L. |
T13955 |
3823-3829 |
NNP |
denotes |
Parada |
T13956 |
3820-3822 |
NNP |
denotes |
F. |
T13957 |
3829-3830 |
-RRB- |
denotes |
) |
T13958 |
3830-3831 |
. |
denotes |
. |
T13959 |
3831-4661 |
sentence |
denotes |
Antibodies used in this study were as follows: rabbit anti-Er81 [14], rabbit anti-Pea3 [14], rabbit anti-PV [14], rabbit anti-eGFP (Molecular Probes, Eugene, Oregon, United States), rabbit anti-Calbindin, rabbit anti-Calretinin (Swant, Bellinzona, Switzerland), rabbit anti-CGRP (Chemicon, Temecula, California, United States), rabbit anti-vGlut1 (Synaptic Systems, Goettingen, Germany), rabbit anti-Brn3a (gift from E. Turner), rabbit anti-TrkA and -p75 (gift from L. F. Reichardt), rabbit anti-Runx3 (Kramer and Arber, unpublished reagent), rabbit anti-Rhodamine (Molecular Probes), mouse anti-neurofilament (American Type Culture Collection, Manassas, Virginia, United States), sheep anti-eGFP (Biogenesis, Poole, United Kingdom), goat anti-LacZ [14], goat anti-TrkC (gift from L. F. Reichardt), and guinea pig anti-Isl1 [14]. |
T13960 |
3832-3842 |
NNS |
denotes |
Antibodies |
T13961 |
3862-3866 |
VBD |
denotes |
were |
T13962 |
3843-3847 |
VBN |
denotes |
used |
T13963 |
3848-3850 |
IN |
denotes |
in |
T13964 |
3851-3855 |
DT |
denotes |
this |
T13965 |
3856-3861 |
NN |
denotes |
study |
T13966 |
3867-3869 |
IN |
denotes |
as |
T13967 |
3870-3877 |
VBZ |
denotes |
follows |
T13968 |
3877-3879 |
: |
denotes |
: |
T13969 |
3879-3885 |
NN |
denotes |
rabbit |
T13970 |
3886-3895 |
NN |
denotes |
anti-Er81 |
T13971 |
3896-3897 |
-LRB- |
denotes |
[ |
T13972 |
3897-3899 |
CD |
denotes |
14 |
T13973 |
3899-3900 |
-RRB- |
denotes |
] |
T13974 |
3900-3902 |
, |
denotes |
, |
T13975 |
3902-3908 |
NN |
denotes |
rabbit |
T13976 |
3909-3918 |
JJ |
denotes |
anti-Pea3 |
T13977 |
3919-3920 |
-LRB- |
denotes |
[ |
T13978 |
3920-3922 |
CD |
denotes |
14 |
T13979 |
3922-3923 |
-RRB- |
denotes |
] |
T13980 |
3923-3925 |
, |
denotes |
, |
T13981 |
3925-3931 |
NN |
denotes |
rabbit |
T13982 |
3932-3939 |
JJ |
denotes |
anti-PV |
T13983 |
3940-3941 |
-LRB- |
denotes |
[ |
T13984 |
3941-3943 |
CD |
denotes |
14 |
T13985 |
3943-3944 |
-RRB- |
denotes |
] |
T13986 |
3944-3946 |
, |
denotes |
, |
T13987 |
3946-3952 |
NN |
denotes |
rabbit |
T13988 |
3953-3962 |
JJ |
denotes |
anti-eGFP |
T13989 |
3963-3964 |
-LRB- |
denotes |
( |
T13990 |
3974-3980 |
NNP |
denotes |
Probes |
T13991 |
3964-3973 |
NNP |
denotes |
Molecular |
T13992 |
3980-3982 |
, |
denotes |
, |
T13993 |
3982-3988 |
NNP |
denotes |
Eugene |
T13994 |
3988-3990 |
, |
denotes |
, |
T13995 |
3990-3996 |
NNP |
denotes |
Oregon |
T13996 |
3996-3998 |
, |
denotes |
, |
T13997 |
3998-4004 |
NNP |
denotes |
United |
T13998 |
4005-4011 |
NNP |
denotes |
States |
T13999 |
4011-4012 |
-RRB- |
denotes |
) |
T14000 |
4012-4014 |
, |
denotes |
, |
T14001 |
4014-4020 |
NN |
denotes |
rabbit |
T14002 |
4021-4035 |
JJ |
denotes |
anti-Calbindin |
T14003 |
4035-4037 |
, |
denotes |
, |
T14004 |
4037-4043 |
NN |
denotes |
rabbit |
T14005 |
4044-4059 |
JJ |
denotes |
anti-Calretinin |
T14006 |
4060-4061 |
-LRB- |
denotes |
( |
T14007 |
4061-4066 |
NNP |
denotes |
Swant |
T14008 |
4066-4068 |
, |
denotes |
, |
T14009 |
4068-4078 |
NNP |
denotes |
Bellinzona |
T14010 |
4078-4080 |
, |
denotes |
, |
T14011 |
4080-4091 |
NNP |
denotes |
Switzerland |
T14012 |
4091-4092 |
-RRB- |
denotes |
) |
T14013 |
4092-4094 |
, |
denotes |
, |
T14014 |
4094-4100 |
NN |
denotes |
rabbit |
T14015 |
4101-4110 |
JJ |
denotes |
anti-CGRP |
T14016 |
4111-4112 |
-LRB- |
denotes |
( |
T14017 |
4112-4120 |
NNP |
denotes |
Chemicon |
T14018 |
4120-4122 |
, |
denotes |
, |
T14019 |
4122-4130 |
NNP |
denotes |
Temecula |
T14020 |
4130-4132 |
, |
denotes |
, |
T14021 |
4132-4142 |
NNP |
denotes |
California |
T14022 |
4142-4144 |
, |
denotes |
, |
T14023 |
4144-4150 |
NNP |
denotes |
United |
T14024 |
4151-4157 |
NNP |
denotes |
States |
T14025 |
4157-4158 |
-RRB- |
denotes |
) |
T14026 |
4158-4160 |
, |
denotes |
, |
T14027 |
4160-4166 |
NN |
denotes |
rabbit |
T14028 |
4167-4178 |
JJ |
denotes |
anti-vGlut1 |
T14029 |
4179-4180 |
-LRB- |
denotes |
( |
T14030 |
4189-4196 |
NNP |
denotes |
Systems |
T14031 |
4180-4188 |
NN |
denotes |
Synaptic |
T14032 |
4196-4198 |
, |
denotes |
, |
T14033 |
4198-4208 |
NNP |
denotes |
Goettingen |
T14034 |
4208-4210 |
, |
denotes |
, |
T14035 |
4210-4217 |
NNP |
denotes |
Germany |
T14036 |
4217-4218 |
-RRB- |
denotes |
) |
T14037 |
4218-4220 |
, |
denotes |
, |
T14038 |
4220-4226 |
NN |
denotes |
rabbit |
T14039 |
4227-4237 |
JJ |
denotes |
anti-Brn3a |
T14040 |
4238-4239 |
-LRB- |
denotes |
( |
T14041 |
4239-4243 |
NN |
denotes |
gift |
T14042 |
4244-4248 |
IN |
denotes |
from |
T14043 |
4249-4251 |
NNP |
denotes |
E. |
T14044 |
4252-4258 |
NNP |
denotes |
Turner |
T14045 |
4258-4259 |
-RRB- |
denotes |
) |
T14046 |
4259-4261 |
, |
denotes |
, |
T14047 |
4261-4267 |
NN |
denotes |
rabbit |
T14048 |
4283-4286 |
NN |
denotes |
p75 |
T14049 |
4268-4277 |
JJ |
denotes |
anti-TrkA |
T14050 |
4278-4281 |
CC |
denotes |
and |
T14051 |
4282-4283 |
HYPH |
denotes |
- |
T14052 |
4287-4288 |
-LRB- |
denotes |
( |
T14053 |
4288-4292 |
NN |
denotes |
gift |
T14054 |
4293-4297 |
IN |
denotes |
from |
T14055 |
4298-4300 |
NNP |
denotes |
L. |
T14056 |
4304-4313 |
NNP |
denotes |
Reichardt |
T14057 |
4301-4303 |
NNP |
denotes |
F. |
T14058 |
4313-4314 |
-RRB- |
denotes |
) |
T14059 |
4314-4316 |
, |
denotes |
, |
T14060 |
4316-4322 |
NN |
denotes |
rabbit |
T14061 |
4323-4333 |
JJ |
denotes |
anti-Runx3 |
T14062 |
4334-4335 |
-LRB- |
denotes |
( |
T14063 |
4335-4341 |
NNP |
denotes |
Kramer |
T14064 |
4342-4345 |
CC |
denotes |
and |
T14065 |
4346-4351 |
NNP |
denotes |
Arber |
T14066 |
4351-4353 |
, |
denotes |
, |
T14067 |
4353-4364 |
JJ |
denotes |
unpublished |
T14068 |
4365-4372 |
NN |
denotes |
reagent |
T14069 |
4372-4373 |
-RRB- |
denotes |
) |
T14070 |
4373-4375 |
, |
denotes |
, |
T14071 |
4375-4381 |
NN |
denotes |
rabbit |
T14072 |
4382-4396 |
JJ |
denotes |
anti-Rhodamine |
T14073 |
4397-4398 |
-LRB- |
denotes |
( |
T14074 |
4408-4414 |
NNP |
denotes |
Probes |
T14075 |
4398-4407 |
NNP |
denotes |
Molecular |
T14076 |
4414-4415 |
-RRB- |
denotes |
) |
T14077 |
4415-4417 |
, |
denotes |
, |
T14078 |
4417-4422 |
NN |
denotes |
mouse |
T14079 |
4423-4441 |
JJ |
denotes |
anti-neurofilament |
T14080 |
4442-4443 |
-LRB- |
denotes |
( |
T14081 |
4465-4475 |
NNP |
denotes |
Collection |
T14082 |
4443-4451 |
NNP |
denotes |
American |
T14083 |
4452-4456 |
NNP |
denotes |
Type |
T14084 |
4457-4464 |
NNP |
denotes |
Culture |
T14085 |
4475-4477 |
, |
denotes |
, |
T14086 |
4477-4485 |
NNP |
denotes |
Manassas |
T14087 |
4485-4487 |
, |
denotes |
, |
T14088 |
4487-4495 |
NNP |
denotes |
Virginia |
T14089 |
4495-4497 |
, |
denotes |
, |
T14090 |
4497-4503 |
NNP |
denotes |
United |
T14091 |
4504-4510 |
NNP |
denotes |
States |
T14092 |
4510-4511 |
-RRB- |
denotes |
) |
T14093 |
4511-4513 |
, |
denotes |
, |
T14094 |
4513-4518 |
NN |
denotes |
sheep |
T14095 |
4519-4528 |
JJ |
denotes |
anti-eGFP |
T14096 |
4529-4530 |
-LRB- |
denotes |
( |
T14097 |
4530-4540 |
NNP |
denotes |
Biogenesis |
T14098 |
4540-4542 |
, |
denotes |
, |
T14099 |
4542-4547 |
NNP |
denotes |
Poole |
T14100 |
4547-4549 |
, |
denotes |
, |
T14101 |
4549-4555 |
NNP |
denotes |
United |
T14102 |
4556-4563 |
NNP |
denotes |
Kingdom |
T14103 |
4563-4564 |
-RRB- |
denotes |
) |
T14104 |
4564-4566 |
, |
denotes |
, |
T14105 |
4566-4570 |
NN |
denotes |
goat |
T14106 |
4571-4580 |
JJ |
denotes |
anti-LacZ |
T14107 |
4581-4582 |
-LRB- |
denotes |
[ |
T14108 |
4582-4584 |
CD |
denotes |
14 |
T14109 |
4584-4585 |
-RRB- |
denotes |
] |
T14110 |
4585-4587 |
, |
denotes |
, |
T14111 |
4587-4591 |
NN |
denotes |
goat |
T14112 |
4592-4601 |
JJ |
denotes |
anti-TrkC |
T14113 |
4602-4603 |
-LRB- |
denotes |
( |
T14114 |
4603-4607 |
NN |
denotes |
gift |
T14115 |
4608-4612 |
IN |
denotes |
from |
T14116 |
4613-4615 |
NNP |
denotes |
L. |
T14117 |
4619-4628 |
NNP |
denotes |
Reichardt |
T14118 |
4616-4618 |
NNP |
denotes |
F. |
T14119 |
4628-4629 |
-RRB- |
denotes |
) |
T14120 |
4629-4631 |
, |
denotes |
, |
T14121 |
4631-4634 |
CC |
denotes |
and |
T14122 |
4635-4641 |
NN |
denotes |
guinea |
T14123 |
4642-4645 |
NN |
denotes |
pig |
T14124 |
4646-4655 |
JJ |
denotes |
anti-Isl1 |
T14125 |
4656-4657 |
-LRB- |
denotes |
[ |
T14126 |
4657-4659 |
CD |
denotes |
14 |
T14127 |
4659-4660 |
-RRB- |
denotes |
] |
T14128 |
4660-4661 |
. |
denotes |
. |
T14129 |
4661-4887 |
sentence |
denotes |
Terminal deoxynucleotidyl transferase-mediated biotinylated UTP nick end labeling (TUNEL) to detect apoptotic cells in E13.5 DRG on cryostat sections was performed as described by the manufacturer (Roche, Basel, Switzerland). |
T14130 |
4662-4670 |
JJ |
denotes |
Terminal |
T14131 |
4688-4699 |
NN |
denotes |
transferase |
T14132 |
4671-4687 |
NN |
denotes |
deoxynucleotidyl |
T14133 |
4700-4708 |
VBN |
denotes |
mediated |
T14134 |
4699-4700 |
HYPH |
denotes |
- |
T14135 |
4735-4743 |
NN |
denotes |
labeling |
T14136 |
4709-4721 |
VBN |
denotes |
biotinylated |
T14137 |
4722-4725 |
NN |
denotes |
UTP |
T14138 |
4726-4730 |
NN |
denotes |
nick |
T14139 |
4731-4734 |
NN |
denotes |
end |
T14140 |
4816-4825 |
VBN |
denotes |
performed |
T14141 |
4744-4745 |
-LRB- |
denotes |
( |
T14142 |
4745-4750 |
NN |
denotes |
TUNEL |
T14143 |
4750-4751 |
-RRB- |
denotes |
) |
T14144 |
4752-4754 |
TO |
denotes |
to |
T14145 |
4755-4761 |
VB |
denotes |
detect |
T14146 |
4762-4771 |
JJ |
denotes |
apoptotic |
T14147 |
4772-4777 |
NNS |
denotes |
cells |
T14148 |
4778-4780 |
IN |
denotes |
in |
T14149 |
4781-4786 |
NN |
denotes |
E13.5 |
T14150 |
4787-4790 |
NN |
denotes |
DRG |
T14151 |
4791-4793 |
IN |
denotes |
on |
T14152 |
4794-4802 |
NN |
denotes |
cryostat |
T14153 |
4803-4811 |
NNS |
denotes |
sections |
T14154 |
4812-4815 |
VBD |
denotes |
was |
T14155 |
4826-4828 |
IN |
denotes |
as |
T14156 |
4829-4838 |
VBN |
denotes |
described |
T14157 |
4839-4841 |
IN |
denotes |
by |
T14158 |
4842-4845 |
DT |
denotes |
the |
T14159 |
4846-4858 |
NN |
denotes |
manufacturer |
T14160 |
4859-4860 |
-LRB- |
denotes |
( |
T14161 |
4860-4865 |
NNP |
denotes |
Roche |
T14162 |
4865-4867 |
, |
denotes |
, |
T14163 |
4867-4872 |
NNP |
denotes |
Basel |
T14164 |
4872-4874 |
, |
denotes |
, |
T14165 |
4874-4885 |
NNP |
denotes |
Switzerland |
T14166 |
4885-4886 |
-RRB- |
denotes |
) |
T14167 |
4886-4887 |
. |
denotes |
. |
T14168 |
4887-4974 |
sentence |
denotes |
Quantitative analysis of TUNEL+ DRG cells was performed essentially as described [27]. |
T14169 |
4888-4900 |
JJ |
denotes |
Quantitative |
T14170 |
4901-4909 |
NN |
denotes |
analysis |
T14171 |
4934-4943 |
VBN |
denotes |
performed |
T14172 |
4910-4912 |
IN |
denotes |
of |
T14173 |
4913-4918 |
NN |
denotes |
TUNEL |
T14174 |
4924-4929 |
NNS |
denotes |
cells |
T14175 |
4918-4919 |
SYM |
denotes |
+ |
T14176 |
4920-4923 |
NN |
denotes |
DRG |
T14177 |
4930-4933 |
VBD |
denotes |
was |
T14178 |
4944-4955 |
RB |
denotes |
essentially |
T14179 |
4959-4968 |
VBN |
denotes |
described |
T14180 |
4956-4958 |
IN |
denotes |
as |
T14181 |
4969-4970 |
-LRB- |
denotes |
[ |
T14182 |
4970-4972 |
CD |
denotes |
27 |
T14183 |
4972-4973 |
-RRB- |
denotes |
] |
T14184 |
4973-4974 |
. |
denotes |
. |
T14185 |
4974-5078 |
sentence |
denotes |
BrdU pulse-chase experiments and LacZ wholemount stainings were performed as previously described [46]. |
T14186 |
4975-4979 |
NN |
denotes |
BrdU |
T14187 |
4992-5003 |
NNS |
denotes |
experiments |
T14188 |
4980-4985 |
NN |
denotes |
pulse |
T14189 |
4986-4991 |
NN |
denotes |
chase |
T14190 |
4985-4986 |
HYPH |
denotes |
- |
T14191 |
5039-5048 |
VBN |
denotes |
performed |
T14192 |
5004-5007 |
CC |
denotes |
and |
T14193 |
5008-5012 |
NN |
denotes |
LacZ |
T14194 |
5024-5033 |
NNS |
denotes |
stainings |
T14195 |
5013-5023 |
NN |
denotes |
wholemount |
T14196 |
5034-5038 |
VBD |
denotes |
were |
T14197 |
5049-5051 |
IN |
denotes |
as |
T14198 |
5063-5072 |
VBN |
denotes |
described |
T14199 |
5052-5062 |
RB |
denotes |
previously |
T14200 |
5073-5074 |
-LRB- |
denotes |
[ |
T14202 |
5076-5077 |
-RRB- |
denotes |
] |
T14203 |
5077-5078 |
. |
denotes |
. |
T14204 |
5078-5350 |
sentence |
denotes |
For anterograde tracing experiments to visualize projections of sensory neurons, rhodamine-conjugated dextran (Molecular Probes) was injected into single lumbar (L3) DRG at E13.5 or applied to whole lumbar dorsal roots (L3) at postnatal day (P) 5 using glass capillaries. |
T14205 |
5079-5082 |
IN |
denotes |
For |
T14206 |
5118-5127 |
VB |
denotes |
visualize |
T14207 |
5083-5094 |
JJ |
denotes |
anterograde |
T14208 |
5103-5114 |
NNS |
denotes |
experiments |
T14209 |
5095-5102 |
NN |
denotes |
tracing |
T14210 |
5115-5117 |
TO |
denotes |
to |
T14211 |
5212-5220 |
VBN |
denotes |
injected |
T14212 |
5128-5139 |
NNS |
denotes |
projections |
T14213 |
5140-5142 |
IN |
denotes |
of |
T14214 |
5143-5150 |
JJ |
denotes |
sensory |
T14215 |
5151-5158 |
NNS |
denotes |
neurons |
T14216 |
5158-5160 |
, |
denotes |
, |
T14217 |
5160-5169 |
NN |
denotes |
rhodamine |
T14218 |
5170-5180 |
VBN |
denotes |
conjugated |
T14219 |
5169-5170 |
HYPH |
denotes |
- |
T14220 |
5181-5188 |
NN |
denotes |
dextran |
T14221 |
5189-5190 |
-LRB- |
denotes |
( |
T14222 |
5200-5206 |
NNP |
denotes |
Probes |
T14223 |
5190-5199 |
NNP |
denotes |
Molecular |
T14224 |
5206-5207 |
-RRB- |
denotes |
) |
T14225 |
5208-5211 |
VBD |
denotes |
was |
T14226 |
5221-5225 |
IN |
denotes |
into |
T14227 |
5226-5232 |
JJ |
denotes |
single |
T14228 |
5245-5248 |
NN |
denotes |
DRG |
T14229 |
5233-5239 |
NN |
denotes |
lumbar |
T14230 |
5240-5241 |
-LRB- |
denotes |
( |
T14231 |
5241-5243 |
NN |
denotes |
L3 |
T14232 |
5243-5244 |
-RRB- |
denotes |
) |
T14233 |
5249-5251 |
IN |
denotes |
at |
T14234 |
5252-5257 |
NN |
denotes |
E13.5 |
T14235 |
5258-5260 |
CC |
denotes |
or |
T14236 |
5261-5268 |
VBN |
denotes |
applied |
T14237 |
5269-5271 |
IN |
denotes |
to |
T14238 |
5272-5277 |
JJ |
denotes |
whole |
T14239 |
5292-5297 |
NNS |
denotes |
roots |
T14240 |
5278-5284 |
JJ |
denotes |
lumbar |
T14241 |
5285-5291 |
JJ |
denotes |
dorsal |
T14242 |
5298-5299 |
-LRB- |
denotes |
( |
T14243 |
5299-5301 |
NN |
denotes |
L3 |
T14244 |
5301-5302 |
-RRB- |
denotes |
) |
T14245 |
5303-5305 |
IN |
denotes |
at |
T14246 |
5306-5315 |
JJ |
denotes |
postnatal |
T14247 |
5316-5319 |
NN |
denotes |
day |
T14248 |
5320-5321 |
-LRB- |
denotes |
( |
T14249 |
5321-5322 |
NN |
denotes |
P |
T14250 |
5322-5323 |
-RRB- |
denotes |
) |
T14251 |
5324-5325 |
CD |
denotes |
5 |
T14252 |
5326-5331 |
VBG |
denotes |
using |
T14253 |
5332-5337 |
NN |
denotes |
glass |
T14254 |
5338-5349 |
NNS |
denotes |
capillaries |
T14255 |
5349-5350 |
. |
denotes |
. |
T14256 |
5350-5427 |
sentence |
denotes |
After injection, animals were incubated for 2–3 h (E13.5) or overnight (P5). |
T14257 |
5351-5356 |
IN |
denotes |
After |
T14258 |
5381-5390 |
VBN |
denotes |
incubated |
T14259 |
5357-5366 |
NN |
denotes |
injection |
T14260 |
5366-5368 |
, |
denotes |
, |
T14261 |
5368-5375 |
NNS |
denotes |
animals |
T14262 |
5376-5380 |
VBD |
denotes |
were |
T14263 |
5391-5394 |
IN |
denotes |
for |
T14264 |
5395-5396 |
CD |
denotes |
2 |
T14265 |
5397-5398 |
CD |
denotes |
3 |
T14266 |
5396-5397 |
SYM |
denotes |
– |
T14267 |
5399-5400 |
NN |
denotes |
h |
T14268 |
5401-5402 |
-LRB- |
denotes |
( |
T14269 |
5402-5407 |
NN |
denotes |
E13.5 |
T14270 |
5407-5408 |
-RRB- |
denotes |
) |
T14271 |
5409-5411 |
CC |
denotes |
or |
T14272 |
5412-5421 |
RB |
denotes |
overnight |
T14273 |
5422-5423 |
-LRB- |
denotes |
( |
T14274 |
5423-5425 |
NN |
denotes |
P5 |
T14275 |
5425-5426 |
-RRB- |
denotes |
) |
T14276 |
5426-5427 |
. |
denotes |
. |
T14277 |
5427-5582 |
sentence |
denotes |
Cryostat sections were processed for immunohistochemistry as described [14] using fluorophore-conjugated secondary antibodies (1:1,000, Molecular Probes). |
T14278 |
5428-5436 |
NN |
denotes |
Cryostat |
T14279 |
5437-5445 |
NNS |
denotes |
sections |
T14280 |
5451-5460 |
VBN |
denotes |
processed |
T14281 |
5446-5450 |
VBD |
denotes |
were |
T14282 |
5461-5464 |
IN |
denotes |
for |
T14283 |
5465-5485 |
NN |
denotes |
immunohistochemistry |
T14284 |
5486-5488 |
IN |
denotes |
as |
T14285 |
5489-5498 |
VBN |
denotes |
described |
T14286 |
5499-5500 |
-LRB- |
denotes |
[ |
T14287 |
5500-5502 |
CD |
denotes |
14 |
T14288 |
5502-5503 |
-RRB- |
denotes |
] |
T14289 |
5504-5509 |
VBG |
denotes |
using |
T14290 |
5510-5521 |
NN |
denotes |
fluorophore |
T14291 |
5522-5532 |
VBN |
denotes |
conjugated |
T14292 |
5521-5522 |
HYPH |
denotes |
- |
T14293 |
5543-5553 |
NNS |
denotes |
antibodies |
T14294 |
5533-5542 |
JJ |
denotes |
secondary |
T14295 |
5554-5555 |
-LRB- |
denotes |
( |
T14296 |
5574-5580 |
NNP |
denotes |
Probes |
T14297 |
5555-5556 |
CD |
denotes |
1 |
T14298 |
5556-5557 |
SYM |
denotes |
: |
T14299 |
5557-5562 |
CD |
denotes |
1,000 |
T14300 |
5562-5564 |
, |
denotes |
, |
T14301 |
5564-5573 |
NNP |
denotes |
Molecular |
T14302 |
5580-5581 |
-RRB- |
denotes |
) |
T14303 |
5581-5582 |
. |
denotes |
. |
T14304 |
5582-5654 |
sentence |
denotes |
Images were collected on an Olympus (Tokyo, Japan) confocal microscope. |
T14305 |
5583-5589 |
NNS |
denotes |
Images |
T14306 |
5595-5604 |
VBN |
denotes |
collected |
T14307 |
5590-5594 |
VBD |
denotes |
were |
T14308 |
5605-5607 |
IN |
denotes |
on |
T14309 |
5608-5610 |
DT |
denotes |
an |
T14310 |
5643-5653 |
NN |
denotes |
microscope |
T14311 |
5611-5618 |
NNP |
denotes |
Olympus |
T14312 |
5619-5620 |
-LRB- |
denotes |
( |
T14313 |
5620-5625 |
NNP |
denotes |
Tokyo |
T14314 |
5625-5627 |
, |
denotes |
, |
T14315 |
5627-5632 |
NNP |
denotes |
Japan |
T14316 |
5632-5633 |
-RRB- |
denotes |
) |
T14317 |
5634-5642 |
JJ |
denotes |
confocal |
T14318 |
5653-5654 |
. |
denotes |
. |
T14319 |
5654-5918 |
sentence |
denotes |
Images from in situ hybridization experiments were collected with an RT-SPOT camera (Diagnostic Instruments, Sterling Heights, Michigan, United States), and Corel (Eden Prairie, Minnesota, United States) Photo Paint 10.0 was used for digital processing of images. |
T14320 |
5655-5661 |
NNS |
denotes |
Images |
T14321 |
5706-5715 |
VBN |
denotes |
collected |
T14322 |
5662-5666 |
IN |
denotes |
from |
T14323 |
5667-5669 |
FW |
denotes |
in |
T14324 |
5670-5674 |
FW |
denotes |
situ |
T14325 |
5689-5700 |
NNS |
denotes |
experiments |
T14326 |
5675-5688 |
NN |
denotes |
hybridization |
T14327 |
5701-5705 |
VBD |
denotes |
were |
T14328 |
5716-5720 |
IN |
denotes |
with |
T14329 |
5721-5723 |
DT |
denotes |
an |
T14330 |
5732-5738 |
NN |
denotes |
camera |
T14331 |
5724-5726 |
NN |
denotes |
RT |
T14332 |
5727-5731 |
NN |
denotes |
SPOT |
T14333 |
5726-5727 |
HYPH |
denotes |
- |
T14334 |
5739-5740 |
-LRB- |
denotes |
( |
T14335 |
5751-5762 |
NNPS |
denotes |
Instruments |
T14336 |
5740-5750 |
JJ |
denotes |
Diagnostic |
T14337 |
5762-5764 |
, |
denotes |
, |
T14338 |
5764-5772 |
NNP |
denotes |
Sterling |
T14339 |
5773-5780 |
NNP |
denotes |
Heights |
T14340 |
5780-5782 |
, |
denotes |
, |
T14341 |
5782-5790 |
NNP |
denotes |
Michigan |
T14342 |
5790-5792 |
, |
denotes |
, |
T14343 |
5792-5798 |
NNP |
denotes |
United |
T14344 |
5799-5805 |
NNP |
denotes |
States |
T14345 |
5805-5806 |
-RRB- |
denotes |
) |
T14346 |
5806-5808 |
, |
denotes |
, |
T14347 |
5808-5811 |
CC |
denotes |
and |
T14348 |
5812-5817 |
NNP |
denotes |
Corel |
T14349 |
5865-5870 |
NNP |
denotes |
Paint |
T14350 |
5818-5819 |
-LRB- |
denotes |
( |
T14351 |
5824-5831 |
NNP |
denotes |
Prairie |
T14352 |
5819-5823 |
NNP |
denotes |
Eden |
T14353 |
5831-5833 |
, |
denotes |
, |
T14354 |
5833-5842 |
NNP |
denotes |
Minnesota |
T14355 |
5842-5844 |
, |
denotes |
, |
T14356 |
5844-5850 |
NNP |
denotes |
United |
T14357 |
5851-5857 |
NNP |
denotes |
States |
T14358 |
5857-5858 |
-RRB- |
denotes |
) |
T14359 |
5859-5864 |
NNP |
denotes |
Photo |
T14360 |
5880-5884 |
VBN |
denotes |
used |
T14361 |
5871-5875 |
CD |
denotes |
10.0 |
T14362 |
5876-5879 |
VBD |
denotes |
was |
T14363 |
5885-5888 |
IN |
denotes |
for |
T14364 |
5889-5896 |
JJ |
denotes |
digital |
T14365 |
5897-5907 |
NN |
denotes |
processing |
T14366 |
5908-5910 |
IN |
denotes |
of |
T14367 |
5911-5917 |
NNS |
denotes |
images |
T14368 |
5917-5918 |
. |
denotes |
. |
T14469 |
5920-5922 |
FW |
denotes |
In |
T14470 |
5923-5928 |
FW |
denotes |
vitro |
T14471 |
5929-5937 |
NNS |
denotes |
cultures |
T14472 |
5938-5940 |
IN |
denotes |
of |
T14473 |
5941-5944 |
NN |
denotes |
DRG |
T14474 |
5944-6356 |
sentence |
denotes |
Individual lumbar DRG were dissected from E13.5 or E14.5 embryos and placed on Matrigel (BD Biosciences, San Jose, California, United States) coated coverslips in DMEM/F12 (Gibco, San Diego, California, United States), 2 mM L-Gln (Gibco), N2 (Gibco), and 1 mg/ml BSA (Sigma, St. Louis, Missouri, United States) without neurotrophins, or supplemented with either NGF (100 ng/ml, Gibco) or NT-3 (20 ng/ml, Sigma). |
T14475 |
5945-5955 |
JJ |
denotes |
Individual |
T14476 |
5963-5966 |
NN |
denotes |
DRG |
T14477 |
5956-5962 |
NN |
denotes |
lumbar |
T14478 |
5972-5981 |
VBN |
denotes |
dissected |
T14479 |
5967-5971 |
VBD |
denotes |
were |
T14480 |
5982-5986 |
IN |
denotes |
from |
T14481 |
5987-5992 |
NN |
denotes |
E13.5 |
T14482 |
6002-6009 |
NNS |
denotes |
embryos |
T14483 |
5993-5995 |
CC |
denotes |
or |
T14484 |
5996-6001 |
NN |
denotes |
E14.5 |
T14485 |
6010-6013 |
CC |
denotes |
and |
T14486 |
6014-6020 |
VBN |
denotes |
placed |
T14487 |
6021-6023 |
IN |
denotes |
on |
T14488 |
6024-6032 |
NNP |
denotes |
Matrigel |
T14489 |
6087-6093 |
VBN |
denotes |
coated |
T14490 |
6033-6034 |
-LRB- |
denotes |
( |
T14491 |
6037-6048 |
NNP |
denotes |
Biosciences |
T14492 |
6034-6036 |
NNP |
denotes |
BD |
T14493 |
6048-6050 |
, |
denotes |
, |
T14494 |
6050-6053 |
NNP |
denotes |
San |
T14495 |
6054-6058 |
NNP |
denotes |
Jose |
T14496 |
6058-6060 |
, |
denotes |
, |
T14497 |
6060-6070 |
NNP |
denotes |
California |
T14498 |
6070-6072 |
, |
denotes |
, |
T14499 |
6072-6078 |
NNP |
denotes |
United |
T14500 |
6079-6085 |
NNP |
denotes |
States |
T14501 |
6085-6086 |
-RRB- |
denotes |
) |
T14502 |
6094-6104 |
NNS |
denotes |
coverslips |
T14503 |
6105-6107 |
IN |
denotes |
in |
T14504 |
6108-6112 |
NN |
denotes |
DMEM |
T14505 |
6113-6116 |
NN |
denotes |
F12 |
T14506 |
6112-6113 |
HYPH |
denotes |
/ |
T14507 |
6117-6118 |
-LRB- |
denotes |
( |
T14508 |
6118-6123 |
NNP |
denotes |
Gibco |
T14509 |
6123-6125 |
, |
denotes |
, |
T14510 |
6125-6128 |
NNP |
denotes |
San |
T14511 |
6129-6134 |
NNP |
denotes |
Diego |
T14512 |
6134-6136 |
, |
denotes |
, |
T14513 |
6136-6146 |
NNP |
denotes |
California |
T14514 |
6146-6148 |
, |
denotes |
, |
T14515 |
6148-6154 |
NNP |
denotes |
United |
T14516 |
6155-6161 |
NNP |
denotes |
States |
T14517 |
6161-6162 |
-RRB- |
denotes |
) |
T14518 |
6162-6164 |
, |
denotes |
, |
T14519 |
6164-6165 |
CD |
denotes |
2 |
T14520 |
6166-6168 |
NN |
denotes |
mM |
T14521 |
6171-6174 |
NN |
denotes |
Gln |
T14522 |
6169-6170 |
NN |
denotes |
L |
T14523 |
6170-6171 |
HYPH |
denotes |
- |
T14524 |
6175-6176 |
-LRB- |
denotes |
( |
T14525 |
6176-6181 |
NNP |
denotes |
Gibco |
T14526 |
6181-6182 |
-RRB- |
denotes |
) |
T14527 |
6182-6184 |
, |
denotes |
, |
T14528 |
6184-6186 |
NN |
denotes |
N2 |
T14529 |
6187-6188 |
-LRB- |
denotes |
( |
T14530 |
6188-6193 |
NNP |
denotes |
Gibco |
T14531 |
6193-6194 |
-RRB- |
denotes |
) |
T14532 |
6194-6196 |
, |
denotes |
, |
T14533 |
6196-6199 |
CC |
denotes |
and |
T14534 |
6200-6201 |
CD |
denotes |
1 |
T14535 |
6202-6204 |
NN |
denotes |
mg |
T14536 |
6208-6211 |
NN |
denotes |
BSA |
T14537 |
6204-6205 |
SYM |
denotes |
/ |
T14538 |
6205-6207 |
NN |
denotes |
ml |
T14539 |
6212-6213 |
-LRB- |
denotes |
( |
T14540 |
6213-6218 |
NNP |
denotes |
Sigma |
T14541 |
6218-6220 |
, |
denotes |
, |
T14542 |
6220-6223 |
NNP |
denotes |
St. |
T14543 |
6224-6229 |
NNP |
denotes |
Louis |
T14544 |
6229-6231 |
, |
denotes |
, |
T14545 |
6231-6239 |
NNP |
denotes |
Missouri |
T14546 |
6239-6241 |
, |
denotes |
, |
T14547 |
6241-6247 |
NNP |
denotes |
United |
T14548 |
6248-6254 |
NNP |
denotes |
States |
T14549 |
6254-6255 |
-RRB- |
denotes |
) |
T14550 |
6256-6263 |
IN |
denotes |
without |
T14551 |
6264-6277 |
NNS |
denotes |
neurotrophins |
T14552 |
6277-6279 |
, |
denotes |
, |
T14553 |
6279-6281 |
CC |
denotes |
or |
T14554 |
6282-6294 |
VBN |
denotes |
supplemented |
T14555 |
6295-6299 |
IN |
denotes |
with |
T14556 |
6300-6306 |
CC |
denotes |
either |
T14557 |
6307-6310 |
NN |
denotes |
NGF |
T14558 |
6311-6312 |
-LRB- |
denotes |
( |
T14559 |
6323-6328 |
NNP |
denotes |
Gibco |
T14560 |
6312-6315 |
CD |
denotes |
100 |
T14561 |
6316-6318 |
NN |
denotes |
ng |
T14562 |
6318-6319 |
SYM |
denotes |
/ |
T14563 |
6319-6321 |
NN |
denotes |
ml |
T14564 |
6321-6323 |
, |
denotes |
, |
T14565 |
6328-6329 |
-RRB- |
denotes |
) |
T14566 |
6330-6332 |
CC |
denotes |
or |
T14567 |
6333-6335 |
NN |
denotes |
NT |
T14568 |
6335-6336 |
HYPH |
denotes |
- |
T14569 |
6336-6337 |
CD |
denotes |
3 |
T14570 |
6338-6339 |
-LRB- |
denotes |
( |
T14571 |
6349-6354 |
NNP |
denotes |
Sigma |
T14572 |
6339-6341 |
CD |
denotes |
20 |
T14573 |
6342-6344 |
NN |
denotes |
ng |
T14574 |
6344-6345 |
SYM |
denotes |
/ |
T14575 |
6345-6347 |
NN |
denotes |
ml |
T14576 |
6347-6349 |
, |
denotes |
, |
T14577 |
6354-6355 |
-RRB- |
denotes |
) |
T14578 |
6355-6356 |
. |
denotes |
. |
T14579 |
6356-6496 |
sentence |
denotes |
DRG explants (n ≥ 20 for each condition) were cultured for 48 h, processed for immunocytochemistry, and analyzed using confocal microscopy. |
T14580 |
6357-6360 |
NN |
denotes |
DRG |
T14581 |
6361-6369 |
NNS |
denotes |
explants |
T14582 |
6403-6411 |
VBN |
denotes |
cultured |
T14583 |
6370-6371 |
-LRB- |
denotes |
( |
T14584 |
6375-6377 |
CD |
denotes |
20 |
T14585 |
6371-6372 |
NN |
denotes |
n |
T14586 |
6373-6374 |
SYM |
denotes |
≥ |
T14587 |
6378-6381 |
IN |
denotes |
for |
T14588 |
6382-6386 |
DT |
denotes |
each |
T14589 |
6387-6396 |
NN |
denotes |
condition |
T14590 |
6396-6397 |
-RRB- |
denotes |
) |
T14591 |
6398-6402 |
VBD |
denotes |
were |
T14592 |
6412-6415 |
IN |
denotes |
for |
T14593 |
6416-6418 |
CD |
denotes |
48 |
T14594 |
6419-6420 |
NN |
denotes |
h |
T14595 |
6420-6422 |
, |
denotes |
, |
T14596 |
6422-6431 |
VBN |
denotes |
processed |
T14597 |
6432-6435 |
IN |
denotes |
for |
T14598 |
6436-6455 |
NN |
denotes |
immunocytochemistry |
T14599 |
6455-6457 |
, |
denotes |
, |
T14600 |
6457-6460 |
CC |
denotes |
and |
T14601 |
6461-6469 |
VBN |
denotes |
analyzed |
T14602 |
6470-6475 |
VBG |
denotes |
using |
T14603 |
6476-6484 |
JJ |
denotes |
confocal |
T14604 |
6485-6495 |
NN |
denotes |
microscopy |
T14605 |
6495-6496 |
. |
denotes |
. |
T14754 |
6498-6505 |
NNP |
denotes |
Western |
T14755 |
6506-6510 |
NN |
denotes |
blot |
T14756 |
6511-6519 |
NN |
denotes |
analysis |
T14757 |
6519-6735 |
sentence |
denotes |
Lumbar DRG from E16.5 embryos were isolated, mechanically disrupted, homogenized using glass beads (Sigma), and lysed in standard lysis buffer supplemented with protease and phosphatase inhibitors as described [47]. |
T14758 |
6520-6526 |
NN |
denotes |
Lumbar |
T14759 |
6527-6530 |
NN |
denotes |
DRG |
T14760 |
6555-6563 |
VBN |
denotes |
isolated |
T14761 |
6531-6535 |
IN |
denotes |
from |
T14762 |
6536-6541 |
NN |
denotes |
E16.5 |
T14763 |
6542-6549 |
NNS |
denotes |
embryos |
T14764 |
6550-6554 |
VBD |
denotes |
were |
T14765 |
6563-6565 |
, |
denotes |
, |
T14766 |
6565-6577 |
RB |
denotes |
mechanically |
T14767 |
6578-6587 |
VBN |
denotes |
disrupted |
T14768 |
6587-6589 |
, |
denotes |
, |
T14769 |
6589-6600 |
VBN |
denotes |
homogenized |
T14770 |
6601-6606 |
VBG |
denotes |
using |
T14771 |
6607-6612 |
NN |
denotes |
glass |
T14772 |
6613-6618 |
NNS |
denotes |
beads |
T14773 |
6619-6620 |
-LRB- |
denotes |
( |
T14774 |
6620-6625 |
NNP |
denotes |
Sigma |
T14775 |
6625-6626 |
-RRB- |
denotes |
) |
T14776 |
6626-6628 |
, |
denotes |
, |
T14777 |
6628-6631 |
CC |
denotes |
and |
T14778 |
6632-6637 |
VBN |
denotes |
lysed |
T14779 |
6638-6640 |
IN |
denotes |
in |
T14780 |
6641-6649 |
JJ |
denotes |
standard |
T14781 |
6656-6662 |
NN |
denotes |
buffer |
T14782 |
6650-6655 |
NN |
denotes |
lysis |
T14783 |
6663-6675 |
VBN |
denotes |
supplemented |
T14784 |
6676-6680 |
IN |
denotes |
with |
T14785 |
6681-6689 |
NN |
denotes |
protease |
T14786 |
6706-6716 |
NNS |
denotes |
inhibitors |
T14787 |
6690-6693 |
CC |
denotes |
and |
T14788 |
6694-6705 |
NN |
denotes |
phosphatase |
T14789 |
6717-6719 |
IN |
denotes |
as |
T14790 |
6720-6729 |
VBN |
denotes |
described |
T14791 |
6730-6731 |
-LRB- |
denotes |
[ |
T14792 |
6731-6733 |
CD |
denotes |
47 |
T14793 |
6733-6734 |
-RRB- |
denotes |
] |
T14794 |
6734-6735 |
. |
denotes |
. |
T14795 |
6735-7025 |
sentence |
denotes |
Protein extracts were resolved by SDS-PAGE, and immunoblotting was performed using antibodies against Akt, p-Akt (Ser473), CREB, p-CREB (Ser133), Bax, Bcl-xl (Cell Signaling Technology, Beverly, Massachusetts, United States), and Bcl2 (BD Pharmingen, San Diego, California, United States). |
T14796 |
6736-6743 |
NN |
denotes |
Protein |
T14797 |
6744-6752 |
NNS |
denotes |
extracts |
T14798 |
6758-6766 |
VBN |
denotes |
resolved |
T14799 |
6753-6757 |
VBD |
denotes |
were |
T14800 |
6767-6769 |
IN |
denotes |
by |
T14801 |
6770-6773 |
NN |
denotes |
SDS |
T14802 |
6774-6778 |
NN |
denotes |
PAGE |
T14803 |
6773-6774 |
HYPH |
denotes |
- |
T14804 |
6778-6780 |
, |
denotes |
, |
T14805 |
6780-6783 |
CC |
denotes |
and |
T14806 |
6784-6798 |
NN |
denotes |
immunoblotting |
T14807 |
6803-6812 |
VBN |
denotes |
performed |
T14808 |
6799-6802 |
VBD |
denotes |
was |
T14809 |
6813-6818 |
VBG |
denotes |
using |
T14810 |
6819-6829 |
NNS |
denotes |
antibodies |
T14811 |
6830-6837 |
IN |
denotes |
against |
T14812 |
6838-6841 |
NN |
denotes |
Akt |
T14813 |
6841-6843 |
, |
denotes |
, |
T14814 |
6843-6844 |
NN |
denotes |
p |
T14815 |
6845-6848 |
NN |
denotes |
Akt |
T14816 |
6844-6845 |
HYPH |
denotes |
- |
T14817 |
6849-6850 |
-LRB- |
denotes |
( |
T14818 |
6850-6856 |
NN |
denotes |
Ser473 |
T14819 |
6856-6857 |
-RRB- |
denotes |
) |
T14820 |
6857-6859 |
, |
denotes |
, |
T14821 |
6859-6863 |
NN |
denotes |
CREB |
T14822 |
6863-6865 |
, |
denotes |
, |
T14823 |
6865-6866 |
NN |
denotes |
p |
T14824 |
6867-6871 |
NN |
denotes |
CREB |
T14825 |
6866-6867 |
HYPH |
denotes |
- |
T14826 |
6872-6873 |
-LRB- |
denotes |
( |
T14827 |
6873-6879 |
NN |
denotes |
Ser133 |
T14828 |
6879-6880 |
-RRB- |
denotes |
) |
T14829 |
6880-6882 |
, |
denotes |
, |
T14830 |
6882-6885 |
NN |
denotes |
Bax |
T14831 |
6885-6887 |
, |
denotes |
, |
T14832 |
6887-6890 |
NN |
denotes |
Bcl |
T14833 |
6891-6893 |
NN |
denotes |
xl |
T14834 |
6890-6891 |
HYPH |
denotes |
- |
T14835 |
6894-6895 |
-LRB- |
denotes |
( |
T14836 |
6910-6920 |
NNP |
denotes |
Technology |
T14837 |
6895-6899 |
NNP |
denotes |
Cell |
T14838 |
6900-6909 |
NNP |
denotes |
Signaling |
T14839 |
6920-6922 |
, |
denotes |
, |
T14840 |
6922-6929 |
NNP |
denotes |
Beverly |
T14841 |
6929-6931 |
, |
denotes |
, |
T14842 |
6931-6944 |
NNP |
denotes |
Massachusetts |
T14843 |
6944-6946 |
, |
denotes |
, |
T14844 |
6946-6952 |
NNP |
denotes |
United |
T14845 |
6953-6959 |
NNP |
denotes |
States |
T14846 |
6959-6960 |
-RRB- |
denotes |
) |
T14847 |
6960-6962 |
, |
denotes |
, |
T14848 |
6962-6965 |
CC |
denotes |
and |
T14849 |
6966-6970 |
NN |
denotes |
Bcl2 |
T14850 |
6971-6972 |
-LRB- |
denotes |
( |
T14851 |
6975-6985 |
NNP |
denotes |
Pharmingen |
T14852 |
6972-6974 |
NN |
denotes |
BD |
T14853 |
6985-6987 |
, |
denotes |
, |
T14854 |
6987-6990 |
NNP |
denotes |
San |
T14855 |
6991-6996 |
NNP |
denotes |
Diego |
T14856 |
6996-6998 |
, |
denotes |
, |
T14857 |
6998-7008 |
NNP |
denotes |
California |
T14858 |
7008-7010 |
, |
denotes |
, |
T14859 |
7010-7016 |
NNP |
denotes |
United |
T14860 |
7017-7023 |
NNP |
denotes |
States |
T14861 |
7023-7024 |
-RRB- |
denotes |
) |
T14862 |
7024-7025 |
. |
denotes |
. |
T14863 |
7025-7228 |
sentence |
denotes |
For quantification, films (X-OMAT AR, Eastman Kodak, Rochester, New York, United States) were scanned and densitometry was performed using IMAGEQUANT 5.2 (Molecular Dynamics, Amersham, Uppsala, Sweden). |
T14864 |
7026-7029 |
IN |
denotes |
For |
T14865 |
7120-7127 |
VBN |
denotes |
scanned |
T14866 |
7030-7044 |
NN |
denotes |
quantification |
T14867 |
7044-7046 |
, |
denotes |
, |
T14868 |
7046-7051 |
NNS |
denotes |
films |
T14869 |
7052-7053 |
-LRB- |
denotes |
( |
T14870 |
7060-7062 |
NNP |
denotes |
AR |
T14871 |
7053-7054 |
NN |
denotes |
X |
T14872 |
7055-7059 |
NN |
denotes |
OMAT |
T14873 |
7054-7055 |
HYPH |
denotes |
- |
T14874 |
7062-7064 |
, |
denotes |
, |
T14875 |
7064-7071 |
NNP |
denotes |
Eastman |
T14876 |
7072-7077 |
NNP |
denotes |
Kodak |
T14877 |
7077-7079 |
, |
denotes |
, |
T14878 |
7079-7088 |
NNP |
denotes |
Rochester |
T14879 |
7088-7090 |
, |
denotes |
, |
T14880 |
7090-7093 |
NNP |
denotes |
New |
T14881 |
7094-7098 |
NNP |
denotes |
York |
T14882 |
7098-7100 |
, |
denotes |
, |
T14883 |
7100-7106 |
NNP |
denotes |
United |
T14884 |
7107-7113 |
NNP |
denotes |
States |
T14885 |
7113-7114 |
-RRB- |
denotes |
) |
T14886 |
7115-7119 |
VBD |
denotes |
were |
T14887 |
7128-7131 |
CC |
denotes |
and |
T14888 |
7132-7144 |
NN |
denotes |
densitometry |
T14889 |
7149-7158 |
VBN |
denotes |
performed |
T14890 |
7145-7148 |
VBD |
denotes |
was |
T14891 |
7159-7164 |
VBG |
denotes |
using |
T14892 |
7165-7175 |
NNP |
denotes |
IMAGEQUANT |
T14893 |
7176-7179 |
CD |
denotes |
5.2 |
T14894 |
7180-7181 |
-LRB- |
denotes |
( |
T14895 |
7191-7199 |
NNP |
denotes |
Dynamics |
T14896 |
7181-7190 |
NNP |
denotes |
Molecular |
T14897 |
7199-7201 |
, |
denotes |
, |
T14898 |
7201-7209 |
NNP |
denotes |
Amersham |
T14899 |
7209-7211 |
, |
denotes |
, |
T14900 |
7211-7218 |
NNP |
denotes |
Uppsala |
T14901 |
7218-7220 |
, |
denotes |
, |
T14902 |
7220-7226 |
NNP |
denotes |
Sweden |
T14903 |
7226-7227 |
-RRB- |
denotes |
) |
T14904 |
7227-7228 |
. |
denotes |
. |
T15020 |
7230-7247 |
NN |
denotes |
Electrophysiology |
T15021 |
7247-7321 |
sentence |
denotes |
Electrophysiological analysis was performed as previously described [48]. |
T15022 |
7248-7268 |
JJ |
denotes |
Electrophysiological |
T15023 |
7269-7277 |
NN |
denotes |
analysis |
T15024 |
7282-7291 |
VBN |
denotes |
performed |
T15025 |
7278-7281 |
VBD |
denotes |
was |
T15026 |
7292-7294 |
IN |
denotes |
as |
T15027 |
7306-7315 |
VBN |
denotes |
described |
T15028 |
7295-7305 |
RB |
denotes |
previously |
T15029 |
7316-7317 |
-LRB- |
denotes |
[ |
T15030 |
7317-7319 |
CD |
denotes |
48 |
T15031 |
7319-7320 |
-RRB- |
denotes |
] |
T15032 |
7320-7321 |
. |
denotes |
. |
T15033 |
7321-7450 |
sentence |
denotes |
Briefly, intracellular recordings from identified quadriceps motor neurons were made using sharp electrodes (75–120 MΩ, 3M KCl). |
T15034 |
7322-7329 |
RB |
denotes |
Briefly |
T15035 |
7402-7406 |
VBN |
denotes |
made |
T15036 |
7329-7331 |
, |
denotes |
, |
T15037 |
7331-7344 |
JJ |
denotes |
intracellular |
T15038 |
7345-7355 |
NNS |
denotes |
recordings |
T15039 |
7356-7360 |
IN |
denotes |
from |
T15040 |
7361-7371 |
VBN |
denotes |
identified |
T15041 |
7389-7396 |
NNS |
denotes |
neurons |
T15042 |
7372-7382 |
NNS |
denotes |
quadriceps |
T15043 |
7383-7388 |
NN |
denotes |
motor |
T15044 |
7397-7401 |
VBD |
denotes |
were |
T15045 |
7407-7412 |
VBG |
denotes |
using |
T15046 |
7413-7418 |
JJ |
denotes |
sharp |
T15047 |
7419-7429 |
NNS |
denotes |
electrodes |
T15048 |
7430-7431 |
-LRB- |
denotes |
( |
T15049 |
7438-7440 |
NN |
denotes |
MΩ |
T15050 |
7431-7433 |
CD |
denotes |
75 |
T15051 |
7434-7437 |
CD |
denotes |
120 |
T15052 |
7433-7434 |
SYM |
denotes |
– |
T15053 |
7440-7442 |
, |
denotes |
, |
T15054 |
7442-7444 |
NN |
denotes |
3M |
T15055 |
7445-7448 |
NN |
denotes |
KCl |
T15056 |
7448-7449 |
-RRB- |
denotes |
) |
T15057 |
7449-7450 |
. |
denotes |
. |
T15058 |
7450-7650 |
sentence |
denotes |
Average responses (10–20 trials) from suprathreshold nerve stimulation (1.5 times the strength that evokes maximal monosynaptic response) of the quadriceps nerve were acquired with LTP software [49]. |
T15059 |
7451-7458 |
JJ |
denotes |
Average |
T15060 |
7459-7468 |
NNS |
denotes |
responses |
T15061 |
7618-7626 |
VBN |
denotes |
acquired |
T15062 |
7469-7470 |
-LRB- |
denotes |
( |
T15063 |
7476-7482 |
NNS |
denotes |
trials |
T15064 |
7470-7472 |
CD |
denotes |
10 |
T15065 |
7473-7475 |
CD |
denotes |
20 |
T15066 |
7472-7473 |
SYM |
denotes |
– |
T15067 |
7482-7483 |
-RRB- |
denotes |
) |
T15068 |
7484-7488 |
IN |
denotes |
from |
T15069 |
7489-7503 |
NN |
denotes |
suprathreshold |
T15070 |
7510-7521 |
NN |
denotes |
stimulation |
T15071 |
7504-7509 |
NN |
denotes |
nerve |
T15072 |
7522-7523 |
-LRB- |
denotes |
( |
T15073 |
7537-7545 |
NN |
denotes |
strength |
T15074 |
7523-7526 |
CD |
denotes |
1.5 |
T15075 |
7527-7532 |
NNS |
denotes |
times |
T15076 |
7533-7536 |
DT |
denotes |
the |
T15077 |
7546-7550 |
WDT |
denotes |
that |
T15078 |
7551-7557 |
VBZ |
denotes |
evokes |
T15079 |
7558-7565 |
JJ |
denotes |
maximal |
T15080 |
7579-7587 |
NN |
denotes |
response |
T15081 |
7566-7578 |
JJ |
denotes |
monosynaptic |
T15082 |
7587-7588 |
-RRB- |
denotes |
) |
T15083 |
7589-7591 |
IN |
denotes |
of |
T15084 |
7592-7595 |
DT |
denotes |
the |
T15085 |
7607-7612 |
NN |
denotes |
nerve |
T15086 |
7596-7606 |
NNS |
denotes |
quadriceps |
T15087 |
7613-7617 |
VBD |
denotes |
were |
T15088 |
7627-7631 |
IN |
denotes |
with |
T15089 |
7632-7635 |
NN |
denotes |
LTP |
T15090 |
7636-7644 |
NN |
denotes |
software |
T15091 |
7645-7646 |
-LRB- |
denotes |
[ |
T15092 |
7646-7648 |
CD |
denotes |
49 |
T15093 |
7648-7649 |
-RRB- |
denotes |
] |
T15094 |
7649-7650 |
. |
denotes |
. |
T15095 |
7650-7748 |
sentence |
denotes |
Only cells with stable resting potentials more negative than −50 mV were considered for analysis. |
T15096 |
7651-7655 |
RB |
denotes |
Only |
T15097 |
7656-7661 |
NNS |
denotes |
cells |
T15098 |
7724-7734 |
VBN |
denotes |
considered |
T15099 |
7662-7666 |
IN |
denotes |
with |
T15100 |
7667-7673 |
JJ |
denotes |
stable |
T15101 |
7682-7692 |
NNS |
denotes |
potentials |
T15102 |
7674-7681 |
NN |
denotes |
resting |
T15103 |
7693-7697 |
RBR |
denotes |
more |
T15104 |
7698-7706 |
JJ |
denotes |
negative |
T15105 |
7707-7711 |
IN |
denotes |
than |
T15106 |
7712-7713 |
SYM |
denotes |
− |
T15107 |
7713-7715 |
CD |
denotes |
50 |
T15108 |
7716-7718 |
NN |
denotes |
mV |
T15109 |
7719-7723 |
VBD |
denotes |
were |
T15110 |
7735-7738 |
IN |
denotes |
for |
T15111 |
7739-7747 |
NN |
denotes |
analysis |
T15112 |
7747-7748 |
. |
denotes |
. |
T15113 |
7748-7928 |
sentence |
denotes |
Monosynaptic amplitudes were determined offline using custom routines in the Matlab environment (The Mathworks, Natick, Massachusetts, United States) as previously described [48]. |
T15114 |
7749-7761 |
JJ |
denotes |
Monosynaptic |
T15115 |
7762-7772 |
NNS |
denotes |
amplitudes |
T15116 |
7778-7788 |
VBN |
denotes |
determined |
T15117 |
7773-7777 |
VBD |
denotes |
were |
T15118 |
7789-7796 |
RB |
denotes |
offline |
T15119 |
7797-7802 |
VBG |
denotes |
using |
T15120 |
7803-7809 |
NN |
denotes |
custom |
T15121 |
7810-7818 |
NNS |
denotes |
routines |
T15122 |
7819-7821 |
IN |
denotes |
in |
T15123 |
7822-7825 |
DT |
denotes |
the |
T15124 |
7833-7844 |
NN |
denotes |
environment |
T15125 |
7826-7832 |
NNP |
denotes |
Matlab |
T15126 |
7845-7846 |
-LRB- |
denotes |
( |
T15127 |
7850-7859 |
NNP |
denotes |
Mathworks |
T15128 |
7846-7849 |
DT |
denotes |
The |
T15129 |
7859-7861 |
, |
denotes |
, |
T15130 |
7861-7867 |
NNP |
denotes |
Natick |
T15131 |
7867-7869 |
, |
denotes |
, |
T15132 |
7869-7882 |
NNP |
denotes |
Massachusetts |
T15133 |
7882-7884 |
, |
denotes |
, |
T15134 |
7884-7890 |
NNP |
denotes |
United |
T15135 |
7891-7897 |
NNP |
denotes |
States |
T15136 |
7897-7898 |
-RRB- |
denotes |
) |
T15137 |
7899-7901 |
IN |
denotes |
as |
T15138 |
7913-7922 |
VBN |
denotes |
described |
T15139 |
7902-7912 |
RB |
denotes |
previously |
T15140 |
7923-7924 |
-LRB- |
denotes |
[ |
T15141 |
7924-7926 |
CD |
denotes |
48 |
T15142 |
7926-7927 |
-RRB- |
denotes |
] |
T15143 |
7927-7928 |
. |
denotes |
. |
T14201 |
5074-5076 |
CD |
denotes |
46 |
R5259 |
T12346 |
T12345 |
prep |
of,Generation |
R5260 |
T12347 |
T12348 |
amod |
transgenic,mice |
R5261 |
T12348 |
T12346 |
pobj |
mice,of |
R5262 |
T12349 |
T12345 |
cc |
and,Generation |
R5263 |
T12350 |
T12351 |
compound |
mouse,genetics |
R5264 |
T12351 |
T12345 |
conj |
genetics,Generation |
R5265 |
T12353 |
T12354 |
compound |
Er81EWS,Pea3 |
R5266 |
T12354 |
T12356 |
compound |
Pea3,mice |
R5267 |
T12355 |
T12354 |
punct |
-,Pea3 |
R5268 |
T12356 |
T12357 |
nsubjpass |
mice,generated |
R5269 |
T12358 |
T12357 |
auxpass |
were,generated |
R5270 |
T12359 |
T12357 |
advcl |
following,generated |
R5271 |
T12360 |
T12361 |
det |
a,strategy |
R5272 |
T12361 |
T12359 |
dobj |
strategy,following |
R5273 |
T12362 |
T12361 |
amod |
similar,strategy |
R5274 |
T12363 |
T12362 |
prep |
to,similar |
R5275 |
T12364 |
T12363 |
pobj |
that,to |
R5276 |
T12365 |
T12364 |
acl |
described,that |
R5277 |
T12366 |
T12365 |
prep |
for,described |
R5278 |
T12367 |
T12368 |
det |
the,generation |
R5279 |
T12368 |
T12366 |
pobj |
generation,for |
R5280 |
T12369 |
T12368 |
prep |
of,generation |
R5281 |
T12370 |
T12371 |
compound |
Er81NLZ,mice |
R5282 |
T12371 |
T12369 |
pobj |
mice,of |
R5283 |
T12372 |
T12373 |
punct |
[,14 |
R5284 |
T12373 |
T12365 |
parataxis |
14,described |
R5285 |
T12374 |
T12373 |
punct |
],14 |
R5286 |
T12375 |
T12357 |
punct |
.,generated |
R5287 |
T12377 |
T12378 |
prep |
In,inserted |
R5288 |
T12379 |
T12377 |
pobj |
brief,In |
R5289 |
T12380 |
T12378 |
punct |
", ",inserted |
R5290 |
T12381 |
T12382 |
det |
a,vector |
R5291 |
T12382 |
T12378 |
nsubjpass |
vector,inserted |
R5292 |
T12383 |
T12382 |
compound |
targeting,vector |
R5293 |
T12384 |
T12382 |
prep |
with,vector |
R5294 |
T12385 |
T12386 |
det |
a,coding |
R5295 |
T12386 |
T12384 |
pobj |
coding,with |
R5296 |
T12387 |
T12386 |
compound |
cDNA,coding |
R5297 |
T12388 |
T12382 |
prep |
for,vector |
R5298 |
T12389 |
T12390 |
compound |
EWS,Pea3 |
R5299 |
T12390 |
T12388 |
pobj |
Pea3,for |
R5300 |
T12391 |
T12390 |
punct |
-,Pea3 |
R5301 |
T12392 |
T12378 |
auxpass |
was,inserted |
R5302 |
T12393 |
T12378 |
prep |
in,inserted |
R5303 |
T12394 |
T12393 |
pobj |
frame,in |
R5304 |
T12395 |
T12394 |
prep |
with,frame |
R5305 |
T12396 |
T12397 |
det |
the,ATG |
R5306 |
T12397 |
T12395 |
pobj |
ATG,with |
R5307 |
T12398 |
T12397 |
amod |
endogenous,ATG |
R5308 |
T12399 |
T12397 |
compound |
start,ATG |
R5309 |
T12400 |
T12378 |
prep |
into,inserted |
R5310 |
T12401 |
T12400 |
pobj |
exon,into |
R5311 |
T12402 |
T12401 |
nummod |
2,exon |
R5312 |
T12403 |
T12401 |
prep |
of,exon |
R5313 |
T12404 |
T12405 |
det |
the,locus |
R5314 |
T12405 |
T12403 |
pobj |
locus,of |
R5315 |
T12406 |
T12405 |
nmod |
Er81,locus |
R5316 |
T12407 |
T12405 |
amod |
genomic,locus |
R5317 |
T12408 |
T12378 |
cc |
and,inserted |
R5318 |
T12409 |
T12378 |
conj |
used,inserted |
R5319 |
T12410 |
T12409 |
prep |
for,used |
R5320 |
T12411 |
T12412 |
amod |
homologous,recombination |
R5321 |
T12412 |
T12410 |
pobj |
recombination,for |
R5322 |
T12413 |
T12409 |
prep |
in,used |
R5323 |
T12414 |
T12415 |
compound |
W95,cells |
R5324 |
T12415 |
T12413 |
pobj |
cells,in |
R5325 |
T12416 |
T12415 |
compound |
ES,cells |
R5326 |
T12417 |
T12378 |
punct |
.,inserted |
R5327 |
T12419 |
T12420 |
compound |
EWS,Pea3 |
R5328 |
T12420 |
T12422 |
nsubj |
Pea3,represents |
R5329 |
T12421 |
T12420 |
punct |
-,Pea3 |
R5330 |
T12423 |
T12424 |
det |
a,gene |
R5331 |
T12424 |
T12422 |
dobj |
gene,represents |
R5332 |
T12425 |
T12424 |
compound |
fusion,gene |
R5333 |
T12426 |
T12424 |
prep |
between,gene |
R5334 |
T12427 |
T12428 |
det |
the,terminal |
R5335 |
T12428 |
T12426 |
pobj |
terminal,between |
R5336 |
T12429 |
T12428 |
compound |
amino,terminal |
R5337 |
T12430 |
T12428 |
prep |
of,terminal |
R5338 |
T12431 |
T12430 |
pobj |
EWS,of |
R5339 |
T12432 |
T12428 |
cc |
and,terminal |
R5340 |
T12433 |
T12434 |
det |
the,domain |
R5341 |
T12434 |
T12428 |
conj |
domain,terminal |
R5342 |
T12435 |
T12434 |
compound |
ETS,domain |
R5343 |
T12436 |
T12434 |
prep |
of,domain |
R5344 |
T12437 |
T12436 |
pobj |
Pea3,of |
R5345 |
T12438 |
T12439 |
punct |
[,20 |
R5346 |
T12439 |
T12422 |
parataxis |
20,represents |
R5347 |
T12440 |
T12439 |
punct |
],20 |
R5348 |
T12441 |
T12422 |
punct |
.,represents |
R5349 |
T12443 |
T12444 |
det |
The,pair |
R5350 |
T12444 |
T12446 |
nsubj |
pair,was |
R5351 |
T12445 |
T12444 |
compound |
primer,pair |
R5352 |
T12447 |
T12444 |
acl |
used,pair |
R5353 |
T12448 |
T12449 |
aux |
to,detect |
R5354 |
T12449 |
T12447 |
advcl |
detect,used |
R5355 |
T12450 |
T12449 |
advmod |
specifically,detect |
R5356 |
T12451 |
T12452 |
det |
the,allele |
R5357 |
T12452 |
T12449 |
dobj |
allele,detect |
R5358 |
T12453 |
T12454 |
compound |
Er81EWS,Pea3 |
R5359 |
T12454 |
T12452 |
compound |
Pea3,allele |
R5360 |
T12455 |
T12454 |
punct |
-,Pea3 |
R5361 |
T12456 |
T12457 |
nummod |
5,CAGCCACTGCACCTACAAGAC |
R5362 |
T12457 |
T12446 |
attr |
CAGCCACTGCACCTACAAGAC,was |
R5363 |
T12458 |
T12456 |
punct |
′,5 |
R5364 |
T12459 |
T12457 |
punct |
-,CAGCCACTGCACCTACAAGAC |
R5365 |
T12460 |
T12457 |
punct |
-,CAGCCACTGCACCTACAAGAC |
R5366 |
T12461 |
T12457 |
nummod |
3,CAGCCACTGCACCTACAAGAC |
R5367 |
T12462 |
T12457 |
punct |
′,CAGCCACTGCACCTACAAGAC |
R5368 |
T12463 |
T12457 |
cc |
and,CAGCCACTGCACCTACAAGAC |
R5369 |
T12464 |
T12465 |
nummod |
5,CTTCCTGCTTGATGTCTCCTTC |
R5370 |
T12465 |
T12457 |
conj |
CTTCCTGCTTGATGTCTCCTTC,CAGCCACTGCACCTACAAGAC |
R5371 |
T12466 |
T12464 |
punct |
′,5 |
R5372 |
T12467 |
T12465 |
punct |
-,CTTCCTGCTTGATGTCTCCTTC |
R5373 |
T12468 |
T12465 |
punct |
-,CTTCCTGCTTGATGTCTCCTTC |
R5374 |
T12469 |
T12465 |
nummod |
3,CTTCCTGCTTGATGTCTCCTTC |
R5375 |
T12470 |
T12465 |
punct |
′,CTTCCTGCTTGATGTCTCCTTC |
R5376 |
T12471 |
T12446 |
punct |
.,was |
R5377 |
T12473 |
T12474 |
prep |
For,integrated |
R5378 |
T12475 |
T12476 |
det |
the,generation |
R5379 |
T12476 |
T12473 |
pobj |
generation,For |
R5380 |
T12477 |
T12476 |
prep |
of,generation |
R5381 |
T12478 |
T12479 |
nmod |
TaumGFP,mice |
R5382 |
T12479 |
T12477 |
pobj |
mice,of |
R5383 |
T12480 |
T12478 |
cc |
and,TaumGFP |
R5384 |
T12481 |
T12482 |
compound |
TauEWS,Pea3 |
R5385 |
T12482 |
T12478 |
conj |
Pea3,TaumGFP |
R5386 |
T12483 |
T12482 |
punct |
-,Pea3 |
R5387 |
T12484 |
T12474 |
punct |
", ",integrated |
R5388 |
T12485 |
T12486 |
nmod |
lox,pA |
R5389 |
T12486 |
T12500 |
nmod |
pA,cassettes |
R5390 |
T12487 |
T12486 |
punct |
-,pA |
R5391 |
T12488 |
T12486 |
nmod |
STOP,pA |
R5392 |
T12489 |
T12486 |
punct |
-,pA |
R5393 |
T12490 |
T12486 |
nmod |
lox,pA |
R5394 |
T12491 |
T12486 |
punct |
-,pA |
R5395 |
T12492 |
T12486 |
nmod |
mGFP,pA |
R5396 |
T12493 |
T12486 |
punct |
-,pA |
R5397 |
T12494 |
T12486 |
nmod |
IRES,pA |
R5398 |
T12495 |
T12486 |
punct |
-,pA |
R5399 |
T12496 |
T12486 |
nmod |
NLS,pA |
R5400 |
T12497 |
T12486 |
punct |
-,pA |
R5401 |
T12498 |
T12486 |
nmod |
LacZ,pA |
R5402 |
T12499 |
T12486 |
punct |
-,pA |
R5403 |
T12500 |
T12474 |
nsubjpass |
cassettes,integrated |
R5404 |
T12501 |
T12486 |
cc |
and,pA |
R5405 |
T12502 |
T12503 |
compound |
lox,pA |
R5406 |
T12503 |
T12486 |
conj |
pA,pA |
R5407 |
T12504 |
T12503 |
punct |
-,pA |
R5408 |
T12505 |
T12503 |
compound |
STOP,pA |
R5409 |
T12506 |
T12503 |
punct |
-,pA |
R5410 |
T12507 |
T12503 |
compound |
lox,pA |
R5411 |
T12508 |
T12503 |
punct |
-,pA |
R5412 |
T12509 |
T12503 |
compound |
EWS,pA |
R5413 |
T12510 |
T12503 |
punct |
-,pA |
R5414 |
T12511 |
T12503 |
compound |
Pea3,pA |
R5415 |
T12512 |
T12503 |
punct |
-,pA |
R5416 |
T12513 |
T12503 |
compound |
IRES,pA |
R5417 |
T12514 |
T12503 |
punct |
-,pA |
R5418 |
T12515 |
T12503 |
compound |
NLS,pA |
R5419 |
T12516 |
T12503 |
punct |
-,pA |
R5420 |
T12517 |
T12503 |
compound |
LacZ,pA |
R5421 |
T12518 |
T12503 |
punct |
-,pA |
R5422 |
T12519 |
T12500 |
compound |
targeting,cassettes |
R5423 |
T12520 |
T12474 |
auxpass |
were,integrated |
R5424 |
T12521 |
T12474 |
prep |
into,integrated |
R5425 |
T12522 |
T12521 |
pobj |
exon,into |
R5426 |
T12523 |
T12522 |
nummod |
2,exon |
R5427 |
T12524 |
T12522 |
prep |
of,exon |
R5428 |
T12525 |
T12526 |
det |
the,locus |
R5429 |
T12526 |
T12524 |
pobj |
locus,of |
R5430 |
T12527 |
T12526 |
nmod |
Tau,locus |
R5431 |
T12528 |
T12526 |
amod |
genomic,locus |
R5432 |
T12529 |
T12530 |
punct |
(,removed |
R5433 |
T12530 |
T12474 |
parataxis |
removed,integrated |
R5434 |
T12531 |
T12532 |
det |
the,ATG |
R5435 |
T12532 |
T12530 |
nsubjpass |
ATG,removed |
R5436 |
T12533 |
T12532 |
amod |
endogenous,ATG |
R5437 |
T12534 |
T12532 |
compound |
start,ATG |
R5438 |
T12535 |
T12530 |
auxpass |
was,removed |
R5439 |
T12536 |
T12530 |
prep |
in,removed |
R5440 |
T12537 |
T12538 |
det |
the,vectors |
R5441 |
T12538 |
T12536 |
pobj |
vectors,in |
R5442 |
T12539 |
T12538 |
compound |
targeting,vectors |
R5443 |
T12540 |
T12530 |
punct |
;,removed |
R5444 |
T12541 |
T12542 |
nsubj |
details,available |
R5445 |
T12542 |
T12530 |
amod |
available,removed |
R5446 |
T12543 |
T12542 |
prep |
upon,available |
R5447 |
T12544 |
T12543 |
pobj |
request,upon |
R5448 |
T12545 |
T12530 |
punct |
),removed |
R5449 |
T12546 |
T12474 |
punct |
.,integrated |
R5450 |
T12548 |
T12549 |
nsubjpass |
mGFP,provided |
R5451 |
T12550 |
T12549 |
auxpass |
was,provided |
R5452 |
T12551 |
T12549 |
agent |
by,provided |
R5453 |
T12552 |
T12553 |
compound |
P.,Caroni |
R5454 |
T12553 |
T12551 |
pobj |
Caroni,by |
R5455 |
T12554 |
T12555 |
punct |
[,25 |
R5456 |
T12555 |
T12549 |
parataxis |
25,provided |
R5457 |
T12556 |
T12555 |
punct |
],25 |
R5458 |
T12557 |
T12549 |
punct |
.,provided |
R5459 |
T12559 |
T12560 |
compound |
ES,recombinants |
R5460 |
T12560 |
T12562 |
nsubjpass |
recombinants,screened |
R5461 |
T12561 |
T12560 |
compound |
cell,recombinants |
R5462 |
T12563 |
T12562 |
auxpass |
were,screened |
R5463 |
T12564 |
T12562 |
prep |
by,screened |
R5464 |
T12565 |
T12566 |
compound |
Southern,blot |
R5465 |
T12566 |
T12567 |
compound |
blot,analysis |
R5466 |
T12567 |
T12564 |
pobj |
analysis,by |
R5467 |
T12568 |
T12562 |
advcl |
using,screened |
R5468 |
T12569 |
T12570 |
det |
the,probe |
R5469 |
T12570 |
T12568 |
dobj |
probe,using |
R5470 |
T12571 |
T12568 |
prep |
in,using |
R5471 |
T12572 |
T12573 |
det |
the,region |
R5472 |
T12573 |
T12571 |
pobj |
region,in |
R5473 |
T12574 |
T12573 |
nummod |
5,region |
R5474 |
T12575 |
T12574 |
punct |
′,5 |
R5475 |
T12576 |
T12577 |
mark |
as,described |
R5476 |
T12577 |
T12568 |
advcl |
described,using |
R5477 |
T12578 |
T12577 |
advmod |
previously,described |
R5478 |
T12579 |
T12580 |
punct |
[,41 |
R5479 |
T12580 |
T12577 |
parataxis |
41,described |
R5480 |
T12581 |
T12580 |
punct |
],41 |
R5481 |
T12582 |
T12562 |
punct |
.,screened |
R5482 |
T12584 |
T12585 |
nsubj |
Frequency,was |
R5483 |
T12586 |
T12584 |
prep |
of,Frequency |
R5484 |
T12587 |
T12586 |
pobj |
recombination,of |
R5485 |
T12588 |
T12587 |
prep |
in,recombination |
R5486 |
T12589 |
T12590 |
nummod |
129,Ola |
R5487 |
T12590 |
T12592 |
compound |
Ola,cells |
R5488 |
T12591 |
T12590 |
punct |
/,Ola |
R5489 |
T12592 |
T12588 |
pobj |
cells,in |
R5490 |
T12593 |
T12592 |
compound |
ES,cells |
R5491 |
T12594 |
T12595 |
advmod |
approximately,3 |
R5492 |
T12595 |
T12585 |
attr |
3,was |
R5493 |
T12596 |
T12595 |
quantmod |
1,3 |
R5494 |
T12597 |
T12595 |
punct |
/,3 |
R5495 |
T12598 |
T12585 |
prep |
for,was |
R5496 |
T12599 |
T12600 |
det |
both,constructs |
R5497 |
T12600 |
T12598 |
pobj |
constructs,for |
R5498 |
T12601 |
T12600 |
compound |
Tau,constructs |
R5499 |
T12602 |
T12585 |
punct |
.,was |
R5500 |
T12604 |
T12605 |
prep |
For,obtained |
R5501 |
T12606 |
T12607 |
det |
the,generation |
R5502 |
T12607 |
T12604 |
pobj |
generation,For |
R5503 |
T12608 |
T12607 |
prep |
of,generation |
R5504 |
T12609 |
T12610 |
compound |
PVCre,mice |
R5505 |
T12610 |
T12608 |
pobj |
mice,of |
R5506 |
T12611 |
T12605 |
punct |
", ",obtained |
R5507 |
T12612 |
T12613 |
nmod |
mouse,clones |
R5508 |
T12613 |
T12605 |
nsubjpass |
clones,obtained |
R5509 |
T12614 |
T12613 |
amod |
genomic,clones |
R5510 |
T12615 |
T12605 |
auxpass |
were,obtained |
R5511 |
T12616 |
T12605 |
prep |
by,obtained |
R5512 |
T12617 |
T12616 |
pcomp |
screening,by |
R5513 |
T12618 |
T12619 |
det |
a,library |
R5514 |
T12619 |
T12617 |
dobj |
library,screening |
R5515 |
T12620 |
T12621 |
nmod |
129SV,J |
R5516 |
T12621 |
T12619 |
nmod |
J,library |
R5517 |
T12622 |
T12621 |
punct |
/,J |
R5518 |
T12623 |
T12619 |
amod |
genomic,library |
R5519 |
T12624 |
T12625 |
punct |
(,Incyte |
R5520 |
T12625 |
T12617 |
parataxis |
Incyte,screening |
R5521 |
T12626 |
T12625 |
punct |
", ",Incyte |
R5522 |
T12627 |
T12625 |
npadvmod |
Wilmington,Incyte |
R5523 |
T12628 |
T12625 |
punct |
", ",Incyte |
R5524 |
T12629 |
T12625 |
npadvmod |
Delaware,Incyte |
R5525 |
T12630 |
T12625 |
punct |
", ",Incyte |
R5526 |
T12631 |
T12632 |
compound |
United,States |
R5527 |
T12632 |
T12625 |
npadvmod |
States,Incyte |
R5528 |
T12633 |
T12625 |
punct |
),Incyte |
R5529 |
T12634 |
T12605 |
punct |
.,obtained |
R5530 |
T12636 |
T12637 |
prep |
For,see |
R5531 |
T12638 |
T12636 |
pobj |
details,For |
R5532 |
T12639 |
T12638 |
prep |
on,details |
R5533 |
T12640 |
T12641 |
det |
the,structure |
R5534 |
T12641 |
T12639 |
pobj |
structure,on |
R5535 |
T12642 |
T12641 |
amod |
genomic,structure |
R5536 |
T12643 |
T12641 |
prep |
of,structure |
R5537 |
T12644 |
T12645 |
det |
the,locus |
R5538 |
T12645 |
T12643 |
pobj |
locus,of |
R5539 |
T12646 |
T12645 |
compound |
mouse,locus |
R5540 |
T12647 |
T12645 |
compound |
PV,locus |
R5541 |
T12648 |
T12637 |
punct |
[,see |
R5542 |
T12649 |
T12637 |
dobj |
42,see |
R5543 |
T12650 |
T12637 |
punct |
],see |
R5544 |
T12651 |
T12637 |
punct |
.,see |
R5545 |
T12653 |
T12654 |
det |
An,cassette |
R5546 |
T12654 |
T12661 |
nsubjpass |
cassette,integrated |
R5547 |
T12655 |
T12656 |
compound |
IRES,pA |
R5548 |
T12656 |
T12654 |
compound |
pA,cassette |
R5549 |
T12657 |
T12656 |
punct |
-,pA |
R5550 |
T12658 |
T12656 |
compound |
Cre,pA |
R5551 |
T12659 |
T12656 |
punct |
-,pA |
R5552 |
T12660 |
T12654 |
compound |
targeting,cassette |
R5553 |
T12662 |
T12663 |
punct |
[,33 |
R5554 |
T12663 |
T12654 |
parataxis |
33,cassette |
R5555 |
T12664 |
T12663 |
punct |
],33 |
R5556 |
T12665 |
T12661 |
auxpass |
was,integrated |
R5557 |
T12666 |
T12661 |
prep |
into,integrated |
R5558 |
T12667 |
T12668 |
det |
the,UTR |
R5559 |
T12668 |
T12666 |
pobj |
UTR,into |
R5560 |
T12669 |
T12668 |
nummod |
3,UTR |
R5561 |
T12670 |
T12669 |
punct |
′,3 |
R5562 |
T12671 |
T12668 |
prep |
of,UTR |
R5563 |
T12672 |
T12671 |
pobj |
exon,of |
R5564 |
T12673 |
T12672 |
nummod |
5,exon |
R5565 |
T12674 |
T12661 |
punct |
", ",integrated |
R5566 |
T12675 |
T12661 |
cc |
and,integrated |
R5567 |
T12676 |
T12677 |
compound |
ES,recombinants |
R5568 |
T12677 |
T12679 |
nsubjpass |
recombinants,screened |
R5569 |
T12678 |
T12677 |
compound |
cell,recombinants |
R5570 |
T12679 |
T12661 |
conj |
screened,integrated |
R5571 |
T12680 |
T12679 |
auxpass |
were,screened |
R5572 |
T12681 |
T12679 |
prep |
with,screened |
R5573 |
T12682 |
T12683 |
det |
a,probe |
R5574 |
T12683 |
T12681 |
pobj |
probe,with |
R5575 |
T12684 |
T12683 |
nummod |
5,probe |
R5576 |
T12685 |
T12684 |
punct |
′,5 |
R5577 |
T12686 |
T12687 |
punct |
(,digest |
R5578 |
T12687 |
T12679 |
parataxis |
digest,screened |
R5579 |
T12688 |
T12687 |
dep |
oligos,digest |
R5580 |
T12689 |
T12688 |
punct |
", ",oligos |
R5581 |
T12690 |
T12691 |
nummod |
5,GAGATGACCCAGCCAGGATGCCTC |
R5582 |
T12691 |
T12688 |
conj |
GAGATGACCCAGCCAGGATGCCTC,oligos |
R5583 |
T12692 |
T12690 |
punct |
′,5 |
R5584 |
T12693 |
T12691 |
punct |
-,GAGATGACCCAGCCAGGATGCCTC |
R5585 |
T12694 |
T12691 |
punct |
-,GAGATGACCCAGCCAGGATGCCTC |
R5586 |
T12695 |
T12691 |
nummod |
3,GAGATGACCCAGCCAGGATGCCTC |
R5587 |
T12696 |
T12691 |
punct |
′,GAGATGACCCAGCCAGGATGCCTC |
R5588 |
T12697 |
T12691 |
cc |
and,GAGATGACCCAGCCAGGATGCCTC |
R5589 |
T12698 |
T12699 |
nummod |
5,CTGACCACTCTCGCTCCGGTGTCC |
R5590 |
T12699 |
T12691 |
conj |
CTGACCACTCTCGCTCCGGTGTCC,GAGATGACCCAGCCAGGATGCCTC |
R5591 |
T12700 |
T12698 |
punct |
′,5 |
R5592 |
T12701 |
T12699 |
punct |
-,CTGACCACTCTCGCTCCGGTGTCC |
R5593 |
T12702 |
T12699 |
punct |
-,CTGACCACTCTCGCTCCGGTGTCC |
R5594 |
T12703 |
T12699 |
nummod |
3,CTGACCACTCTCGCTCCGGTGTCC |
R5595 |
T12704 |
T12699 |
punct |
′,CTGACCACTCTCGCTCCGGTGTCC |
R5596 |
T12705 |
T12687 |
punct |
;,digest |
R5597 |
T12706 |
T12707 |
amod |
genomic,DNA |
R5598 |
T12707 |
T12687 |
dep |
DNA,digest |
R5599 |
T12708 |
T12687 |
punct |
", ",digest |
R5600 |
T12709 |
T12687 |
compound |
HindIII,digest |
R5601 |
T12710 |
T12687 |
punct |
),digest |
R5602 |
T12711 |
T12679 |
punct |
.,screened |
R5603 |
T12713 |
T12714 |
det |
The,frequency |
R5604 |
T12714 |
T12715 |
nsubj |
frequency,was |
R5605 |
T12716 |
T12714 |
prep |
of,frequency |
R5606 |
T12717 |
T12716 |
pobj |
recombination,of |
R5607 |
T12718 |
T12717 |
prep |
in,recombination |
R5608 |
T12719 |
T12720 |
nummod |
129,Ola |
R5609 |
T12720 |
T12722 |
compound |
Ola,cells |
R5610 |
T12721 |
T12720 |
punct |
/,Ola |
R5611 |
T12722 |
T12718 |
pobj |
cells,in |
R5612 |
T12723 |
T12722 |
compound |
ES,cells |
R5613 |
T12724 |
T12725 |
advmod |
approximately,20 |
R5614 |
T12725 |
T12715 |
attr |
20,was |
R5615 |
T12726 |
T12725 |
quantmod |
1,20 |
R5616 |
T12727 |
T12725 |
punct |
/,20 |
R5617 |
T12728 |
T12715 |
punct |
.,was |
R5618 |
T12730 |
T12731 |
amod |
Recombinant,clones |
R5619 |
T12731 |
T12732 |
nsubjpass |
clones,aggregated |
R5620 |
T12733 |
T12732 |
auxpass |
were,aggregated |
R5621 |
T12734 |
T12732 |
prep |
with,aggregated |
R5622 |
T12735 |
T12736 |
compound |
morula,stage |
R5623 |
T12736 |
T12737 |
compound |
stage,embryos |
R5624 |
T12737 |
T12734 |
pobj |
embryos,with |
R5625 |
T12738 |
T12739 |
aux |
to,generate |
R5626 |
T12739 |
T12732 |
advcl |
generate,aggregated |
R5627 |
T12740 |
T12741 |
amod |
chimeric,mice |
R5628 |
T12741 |
T12739 |
dobj |
mice,generate |
R5629 |
T12742 |
T12741 |
compound |
founder,mice |
R5630 |
T12743 |
T12744 |
dep |
that,transmitted |
R5631 |
T12744 |
T12741 |
relcl |
transmitted,mice |
R5632 |
T12745 |
T12746 |
det |
the,alleles |
R5633 |
T12746 |
T12744 |
dobj |
alleles,transmitted |
R5634 |
T12747 |
T12746 |
compound |
mutant,alleles |
R5635 |
T12748 |
T12732 |
punct |
.,aggregated |
R5636 |
T12750 |
T12751 |
prep |
In,were |
R5637 |
T12752 |
T12753 |
det |
all,experiments |
R5638 |
T12753 |
T12750 |
pobj |
experiments,In |
R5639 |
T12754 |
T12753 |
acl |
performed,experiments |
R5640 |
T12755 |
T12754 |
prep |
in,performed |
R5641 |
T12756 |
T12757 |
det |
this,study |
R5642 |
T12757 |
T12755 |
pobj |
study,in |
R5643 |
T12758 |
T12751 |
punct |
", ",were |
R5644 |
T12759 |
T12751 |
nsubj |
animals,were |
R5645 |
T12760 |
T12751 |
prep |
of,were |
R5646 |
T12761 |
T12762 |
amod |
mixed,background |
R5647 |
T12762 |
T12760 |
pobj |
background,of |
R5648 |
T12763 |
T12762 |
amod |
genetic,background |
R5649 |
T12764 |
T12765 |
punct |
(,Ola |
R5650 |
T12765 |
T12751 |
parataxis |
Ola,were |
R5651 |
T12766 |
T12765 |
nummod |
129,Ola |
R5652 |
T12767 |
T12765 |
punct |
/,Ola |
R5653 |
T12768 |
T12765 |
cc |
and,Ola |
R5654 |
T12769 |
T12765 |
conj |
C57Bl6,Ola |
R5655 |
T12770 |
T12765 |
punct |
),Ola |
R5656 |
T12771 |
T12751 |
punct |
.,were |
R5657 |
T12773 |
T12774 |
nmod |
Thy1spGFP,mice |
R5658 |
T12774 |
T12776 |
nsubjpass |
mice,generated |
R5659 |
T12775 |
T12774 |
amod |
transgenic,mice |
R5660 |
T12777 |
T12776 |
auxpass |
were,generated |
R5661 |
T12778 |
T12776 |
prep |
in,generated |
R5662 |
T12779 |
T12778 |
pobj |
analogy,in |
R5663 |
T12780 |
T12779 |
prep |
to,analogy |
R5664 |
T12781 |
T12782 |
compound |
De,Paola |
R5665 |
T12782 |
T12780 |
pobj |
Paola,to |
R5666 |
T12783 |
T12784 |
advmod |
et,al. |
R5667 |
T12784 |
T12782 |
advmod |
al.,Paola |
R5668 |
T12785 |
T12786 |
punct |
[,25 |
R5669 |
T12786 |
T12776 |
parataxis |
25,generated |
R5670 |
T12787 |
T12786 |
punct |
],25 |
R5671 |
T12788 |
T12776 |
punct |
", ",generated |
R5672 |
T12789 |
T12776 |
cc |
and,generated |
R5673 |
T12790 |
T12791 |
prep |
for,selected |
R5674 |
T12791 |
T12776 |
conj |
selected,generated |
R5675 |
T12792 |
T12793 |
det |
these,experiments |
R5676 |
T12793 |
T12790 |
pobj |
experiments,for |
R5677 |
T12794 |
T12795 |
det |
a,strain |
R5678 |
T12795 |
T12791 |
nsubjpass |
strain,selected |
R5679 |
T12796 |
T12795 |
prep |
of,strain |
R5680 |
T12797 |
T12796 |
pobj |
mice,of |
R5681 |
T12798 |
T12797 |
prep |
with,mice |
R5682 |
T12799 |
T12800 |
amod |
early,expression |
R5683 |
T12800 |
T12798 |
pobj |
expression,with |
R5684 |
T12801 |
T12800 |
amod |
embryonic,expression |
R5685 |
T12802 |
T12791 |
auxpass |
was,selected |
R5686 |
T12803 |
T12791 |
punct |
.,selected |
R5687 |
T12805 |
T12806 |
nmod |
Isl1Cre,strains |
R5688 |
T12806 |
T12810 |
nsubjpass |
strains,described |
R5689 |
T12807 |
T12805 |
cc |
and,Isl1Cre |
R5690 |
T12808 |
T12805 |
conj |
Hb9Cre,Isl1Cre |
R5691 |
T12809 |
T12806 |
compound |
mouse,strains |
R5692 |
T12811 |
T12810 |
aux |
have,described |
R5693 |
T12812 |
T12810 |
auxpass |
been,described |
R5694 |
T12813 |
T12814 |
punct |
[,43 |
R5695 |
T12814 |
T12810 |
parataxis |
43,described |
R5696 |
T12815 |
T12814 |
nummod |
33,43 |
R5697 |
T12816 |
T12814 |
punct |
",",43 |
R5698 |
T12817 |
T12814 |
punct |
],43 |
R5699 |
T12818 |
T12810 |
cc |
and,described |
R5700 |
T12819 |
T12820 |
nmod |
Bax,animals |
R5701 |
T12820 |
T12824 |
nsubj |
animals,were |
R5702 |
T12821 |
T12819 |
punct |
+,Bax |
R5703 |
T12822 |
T12819 |
punct |
/,Bax |
R5704 |
T12823 |
T12819 |
punct |
−,Bax |
R5705 |
T12824 |
T12810 |
conj |
were,described |
R5706 |
T12825 |
T12824 |
prep |
from,were |
R5707 |
T12826 |
T12827 |
compound |
Jackson,Laboratory |
R5708 |
T12827 |
T12825 |
pobj |
Laboratory,from |
R5709 |
T12828 |
T12829 |
punct |
(,Harbor |
R5710 |
T12829 |
T12824 |
parataxis |
Harbor,were |
R5711 |
T12830 |
T12829 |
compound |
Bar,Harbor |
R5712 |
T12831 |
T12829 |
punct |
", ",Harbor |
R5713 |
T12832 |
T12829 |
npadvmod |
Maine,Harbor |
R5714 |
T12833 |
T12829 |
punct |
", ",Harbor |
R5715 |
T12834 |
T12835 |
compound |
United,States |
R5716 |
T12835 |
T12829 |
npadvmod |
States,Harbor |
R5717 |
T12836 |
T12829 |
punct |
),Harbor |
R5718 |
T12837 |
T12838 |
punct |
[,27 |
R5719 |
T12838 |
T12824 |
parataxis |
27,were |
R5720 |
T12839 |
T12838 |
punct |
],27 |
R5721 |
T12840 |
T12824 |
punct |
.,were |
R5722 |
T12842 |
T12843 |
amod |
Timed,pregnancies |
R5723 |
T12843 |
T12844 |
nsubjpass |
pregnancies,set |
R5724 |
T12845 |
T12844 |
auxpass |
were,set |
R5725 |
T12846 |
T12844 |
prt |
up,set |
R5726 |
T12847 |
T12848 |
aux |
to,generate |
R5727 |
T12848 |
T12844 |
advcl |
generate,set |
R5728 |
T12849 |
T12848 |
dobj |
embryos,generate |
R5729 |
T12850 |
T12849 |
prep |
of,embryos |
R5730 |
T12851 |
T12852 |
amod |
different,stages |
R5731 |
T12852 |
T12850 |
pobj |
stages,of |
R5732 |
T12853 |
T12852 |
amod |
developmental,stages |
R5733 |
T12854 |
T12855 |
mark |
with,described |
R5734 |
T12855 |
T12844 |
advcl |
described,set |
R5735 |
T12856 |
T12857 |
det |
all,genotypes |
R5736 |
T12857 |
T12855 |
nsubj |
genotypes,described |
R5737 |
T12858 |
T12855 |
prep |
throughout,described |
R5738 |
T12859 |
T12860 |
det |
the,study |
R5739 |
T12860 |
T12858 |
pobj |
study,throughout |
R5740 |
T12861 |
T12844 |
punct |
.,set |
R5741 |
T13099 |
T13100 |
amod |
Transcriptional,transactivation |
R5742 |
T13100 |
T13101 |
compound |
transactivation,assays |
R5743 |
T13103 |
T13104 |
det |
The,plasmids |
R5744 |
T13104 |
T13106 |
nsubjpass |
plasmids,used |
R5745 |
T13105 |
T13104 |
amod |
following,plasmids |
R5746 |
T13107 |
T13106 |
auxpass |
were,used |
R5747 |
T13108 |
T13106 |
prep |
for,used |
R5748 |
T13109 |
T13110 |
amod |
transcriptional,assays |
R5749 |
T13110 |
T13108 |
pobj |
assays,for |
R5750 |
T13111 |
T13110 |
compound |
transactivation,assays |
R5751 |
T13112 |
T13106 |
punct |
: ,used |
R5752 |
T13113 |
T13114 |
compound |
pRc,RSV |
R5753 |
T13114 |
T13106 |
dobj |
RSV,used |
R5754 |
T13115 |
T13114 |
punct |
/,RSV |
R5755 |
T13116 |
T13117 |
punct |
(,Invitrogen |
R5756 |
T13117 |
T13114 |
parataxis |
Invitrogen,RSV |
R5757 |
T13118 |
T13117 |
punct |
", ",Invitrogen |
R5758 |
T13119 |
T13117 |
npadvmod |
Carlsbad,Invitrogen |
R5759 |
T13120 |
T13117 |
punct |
", ",Invitrogen |
R5760 |
T13121 |
T13117 |
npadvmod |
California,Invitrogen |
R5761 |
T13122 |
T13117 |
punct |
", ",Invitrogen |
R5762 |
T13123 |
T13124 |
compound |
United,States |
R5763 |
T13124 |
T13117 |
npadvmod |
States,Invitrogen |
R5764 |
T13125 |
T13117 |
punct |
),Invitrogen |
R5765 |
T13126 |
T13114 |
punct |
", ",RSV |
R5766 |
T13127 |
T13128 |
compound |
pRc,RSV |
R5767 |
T13128 |
T13130 |
compound |
RSV,Er81 |
R5768 |
T13129 |
T13128 |
punct |
/,RSV |
R5769 |
T13130 |
T13114 |
conj |
Er81,RSV |
R5770 |
T13131 |
T13130 |
punct |
-,Er81 |
R5771 |
T13132 |
T13130 |
punct |
", ",Er81 |
R5772 |
T13133 |
T13134 |
compound |
pRc,RSV |
R5773 |
T13134 |
T13136 |
compound |
RSV,Pea3 |
R5774 |
T13135 |
T13134 |
punct |
/,RSV |
R5775 |
T13136 |
T13130 |
conj |
Pea3,Er81 |
R5776 |
T13137 |
T13136 |
punct |
-,Pea3 |
R5777 |
T13138 |
T13136 |
compound |
EWS,Pea3 |
R5778 |
T13139 |
T13136 |
punct |
-,Pea3 |
R5779 |
T13140 |
T13136 |
punct |
", ",Pea3 |
R5780 |
T13141 |
T13142 |
compound |
pTP,5xETS |
R5781 |
T13142 |
T13136 |
conj |
5xETS,Pea3 |
R5782 |
T13143 |
T13142 |
punct |
-,5xETS |
R5783 |
T13144 |
T13142 |
punct |
", ",5xETS |
R5784 |
T13145 |
T13142 |
cc |
and,5xETS |
R5785 |
T13146 |
T13147 |
compound |
pTP,5xETSmut |
R5786 |
T13147 |
T13142 |
conj |
5xETSmut,5xETS |
R5787 |
T13148 |
T13147 |
punct |
-,5xETSmut |
R5788 |
T13149 |
T13106 |
punct |
.,used |
R5789 |
T13151 |
T13152 |
compound |
pRc,RSV |
R5790 |
T13152 |
T13154 |
compound |
RSV,Er81 |
R5791 |
T13153 |
T13152 |
punct |
/,RSV |
R5792 |
T13154 |
T13156 |
nsubjpass |
Er81,obtained |
R5793 |
T13155 |
T13154 |
punct |
-,Er81 |
R5794 |
T13157 |
T13154 |
cc |
and,Er81 |
R5795 |
T13158 |
T13159 |
compound |
pRc,RSV |
R5796 |
T13159 |
T13161 |
compound |
RSV,Pea3 |
R5797 |
T13160 |
T13159 |
punct |
/,RSV |
R5798 |
T13161 |
T13154 |
conj |
Pea3,Er81 |
R5799 |
T13162 |
T13161 |
punct |
-,Pea3 |
R5800 |
T13163 |
T13161 |
compound |
EWS,Pea3 |
R5801 |
T13164 |
T13161 |
punct |
-,Pea3 |
R5802 |
T13165 |
T13156 |
auxpass |
were,obtained |
R5803 |
T13166 |
T13156 |
prep |
by,obtained |
R5804 |
T13167 |
T13166 |
pobj |
insertion,by |
R5805 |
T13168 |
T13167 |
prep |
of,insertion |
R5806 |
T13169 |
T13170 |
det |
the,cDNAs |
R5807 |
T13170 |
T13168 |
pobj |
cDNAs,of |
R5808 |
T13171 |
T13170 |
prep |
for,cDNAs |
R5809 |
T13172 |
T13171 |
pobj |
Er81,for |
R5810 |
T13173 |
T13172 |
cc |
or,Er81 |
R5811 |
T13174 |
T13175 |
compound |
EWS,Pea3 |
R5812 |
T13175 |
T13172 |
conj |
Pea3,Er81 |
R5813 |
T13176 |
T13175 |
punct |
-,Pea3 |
R5814 |
T13177 |
T13178 |
punct |
(,gift |
R5815 |
T13178 |
T13175 |
parataxis |
gift,Pea3 |
R5816 |
T13179 |
T13178 |
prep |
from,gift |
R5817 |
T13180 |
T13181 |
compound |
J.,Hassell |
R5818 |
T13181 |
T13179 |
pobj |
Hassell,from |
R5819 |
T13182 |
T13181 |
compound |
A.,Hassell |
R5820 |
T13183 |
T13178 |
punct |
),gift |
R5821 |
T13184 |
T13167 |
prep |
into,insertion |
R5822 |
T13185 |
T13186 |
compound |
pRc,RSV |
R5823 |
T13186 |
T13184 |
pobj |
RSV,into |
R5824 |
T13187 |
T13186 |
punct |
/,RSV |
R5825 |
T13188 |
T13156 |
punct |
.,obtained |
R5826 |
T13190 |
T13191 |
compound |
pTP,5xETS |
R5827 |
T13191 |
T13193 |
nsubjpass |
5xETS,constructed |
R5828 |
T13192 |
T13191 |
punct |
-,5xETS |
R5829 |
T13194 |
T13193 |
auxpass |
was,constructed |
R5830 |
T13195 |
T13193 |
prep |
by,constructed |
R5831 |
T13196 |
T13195 |
pcomp |
inserting,by |
R5832 |
T13197 |
T13198 |
det |
a,cassette |
R5833 |
T13198 |
T13196 |
dobj |
cassette,inserting |
R5834 |
T13199 |
T13198 |
prep |
of,cassette |
R5835 |
T13200 |
T13201 |
nummod |
five,copies |
R5836 |
T13201 |
T13199 |
pobj |
copies,of |
R5837 |
T13202 |
T13201 |
amod |
repetitive,copies |
R5838 |
T13203 |
T13201 |
prep |
of,copies |
R5839 |
T13204 |
T13205 |
amod |
high,affinity |
R5840 |
T13205 |
T13207 |
compound |
affinity,sites |
R5841 |
T13206 |
T13205 |
punct |
-,affinity |
R5842 |
T13207 |
T13203 |
pobj |
sites,of |
R5843 |
T13208 |
T13207 |
compound |
Pea3,sites |
R5844 |
T13209 |
T13207 |
compound |
binding,sites |
R5845 |
T13210 |
T13211 |
punct |
(,3 |
R5846 |
T13211 |
T13207 |
parataxis |
3,sites |
R5847 |
T13212 |
T13211 |
dep |
5,3 |
R5848 |
T13213 |
T13212 |
punct |
′,5 |
R5849 |
T13214 |
T13211 |
punct |
-,3 |
R5850 |
T13215 |
T13211 |
dep |
GCCGGAAGC,3 |
R5851 |
T13216 |
T13211 |
punct |
-,3 |
R5852 |
T13217 |
T13211 |
punct |
′,3 |
R5853 |
T13218 |
T13211 |
punct |
),3 |
R5854 |
T13219 |
T13220 |
punct |
[,19 |
R5855 |
T13220 |
T13207 |
parataxis |
19,sites |
R5856 |
T13221 |
T13220 |
nummod |
18,19 |
R5857 |
T13222 |
T13220 |
punct |
",",19 |
R5858 |
T13223 |
T13220 |
punct |
],19 |
R5859 |
T13224 |
T13196 |
prep |
into,inserting |
R5860 |
T13225 |
T13226 |
det |
a,version |
R5861 |
T13226 |
T13224 |
pobj |
version,into |
R5862 |
T13227 |
T13226 |
amod |
modified,version |
R5863 |
T13228 |
T13226 |
prep |
of,version |
R5864 |
T13229 |
T13230 |
compound |
pTK,Luc |
R5865 |
T13230 |
T13228 |
pobj |
Luc,of |
R5866 |
T13231 |
T13230 |
punct |
-,Luc |
R5867 |
T13232 |
T13193 |
punct |
.,constructed |
R5868 |
T13234 |
T13235 |
compound |
pTP,5xETSmut |
R5869 |
T13235 |
T13237 |
nsubjpass |
5xETSmut,generated |
R5870 |
T13236 |
T13235 |
punct |
-,5xETSmut |
R5871 |
T13238 |
T13237 |
auxpass |
was,generated |
R5872 |
T13239 |
T13237 |
prep |
as,generated |
R5873 |
T13240 |
T13241 |
compound |
pTP,5xETS |
R5874 |
T13241 |
T13239 |
pobj |
5xETS,as |
R5875 |
T13242 |
T13241 |
punct |
-,5xETS |
R5876 |
T13243 |
T13239 |
cc |
but,as |
R5877 |
T13244 |
T13239 |
conj |
using,as |
R5878 |
T13245 |
T13246 |
det |
a,complement |
R5879 |
T13246 |
T13244 |
dobj |
complement,using |
R5880 |
T13247 |
T13246 |
amod |
mutated,complement |
R5881 |
T13248 |
T13246 |
prep |
of,complement |
R5882 |
T13249 |
T13250 |
det |
the,sites |
R5883 |
T13250 |
T13248 |
pobj |
sites,of |
R5884 |
T13251 |
T13250 |
compound |
Pea3,sites |
R5885 |
T13252 |
T13250 |
compound |
binding,sites |
R5886 |
T13253 |
T13254 |
punct |
(,3 |
R5887 |
T13254 |
T13246 |
parataxis |
3,complement |
R5888 |
T13255 |
T13254 |
dep |
5,3 |
R5889 |
T13256 |
T13255 |
punct |
′,5 |
R5890 |
T13257 |
T13254 |
punct |
-,3 |
R5891 |
T13258 |
T13254 |
dep |
GCCTATGGC,3 |
R5892 |
T13259 |
T13254 |
punct |
-,3 |
R5893 |
T13260 |
T13254 |
punct |
′,3 |
R5894 |
T13261 |
T13254 |
punct |
),3 |
R5895 |
T13262 |
T13237 |
punct |
.,generated |
R5896 |
T13264 |
T13265 |
det |
A,plasmid |
R5897 |
T13265 |
T13267 |
nsubj |
plasmid,were |
R5898 |
T13266 |
T13265 |
compound |
control,plasmid |
R5899 |
T13268 |
T13269 |
aux |
to,normalize |
R5900 |
T13269 |
T13265 |
advcl |
normalize,plasmid |
R5901 |
T13270 |
T13269 |
prep |
for,normalize |
R5902 |
T13271 |
T13272 |
compound |
transfection,efficiency |
R5903 |
T13272 |
T13270 |
pobj |
efficiency,for |
R5904 |
T13273 |
T13274 |
punct |
(,placZ |
R5905 |
T13274 |
T13272 |
parataxis |
placZ,efficiency |
R5906 |
T13275 |
T13274 |
punct |
),placZ |
R5907 |
T13276 |
T13272 |
cc |
and,efficiency |
R5908 |
T13277 |
T13278 |
compound |
pTK,Luc |
R5909 |
T13278 |
T13272 |
conj |
Luc,efficiency |
R5910 |
T13279 |
T13278 |
punct |
-,Luc |
R5911 |
T13280 |
T13281 |
det |
a,gift |
R5912 |
T13281 |
T13267 |
attr |
gift,were |
R5913 |
T13282 |
T13281 |
prep |
from,gift |
R5914 |
T13283 |
T13284 |
compound |
D.,Kressler |
R5915 |
T13284 |
T13282 |
pobj |
Kressler,from |
R5916 |
T13285 |
T13267 |
punct |
.,were |
R5917 |
T13287 |
T13288 |
nmod |
COS,cells |
R5918 |
T13288 |
T13291 |
nsubjpass |
cells,co-transfected |
R5919 |
T13289 |
T13287 |
punct |
-,COS |
R5920 |
T13290 |
T13287 |
nummod |
7,COS |
R5921 |
T13292 |
T13291 |
auxpass |
were,co-transfected |
R5922 |
T13293 |
T13291 |
prep |
with,co-transfected |
R5923 |
T13294 |
T13295 |
quantmod |
1,1.2 |
R5924 |
T13295 |
T13297 |
nummod |
1.2,μg |
R5925 |
T13296 |
T13295 |
punct |
–,1.2 |
R5926 |
T13297 |
T13293 |
pobj |
μg,with |
R5927 |
T13298 |
T13297 |
prep |
of,μg |
R5928 |
T13299 |
T13300 |
amod |
total,DNA |
R5929 |
T13300 |
T13298 |
pobj |
DNA,of |
R5930 |
T13301 |
T13297 |
prep |
including,μg |
R5931 |
T13302 |
T13301 |
pobj |
one,including |
R5932 |
T13303 |
T13302 |
prep |
of,one |
R5933 |
T13304 |
T13305 |
det |
the,plasmids |
R5934 |
T13305 |
T13303 |
pobj |
plasmids,of |
R5935 |
T13306 |
T13305 |
compound |
effector,plasmids |
R5936 |
T13307 |
T13308 |
compound |
pRc,RSV |
R5937 |
T13308 |
T13302 |
appos |
RSV,one |
R5938 |
T13309 |
T13308 |
punct |
/,RSV |
R5939 |
T13310 |
T13308 |
punct |
-,RSV |
R5940 |
T13311 |
T13308 |
amod |
empty,RSV |
R5941 |
T13312 |
T13308 |
punct |
", ",RSV |
R5942 |
T13313 |
T13314 |
compound |
pRc,RSV |
R5943 |
T13314 |
T13316 |
compound |
RSV,Er81 |
R5944 |
T13315 |
T13314 |
punct |
/,RSV |
R5945 |
T13316 |
T13308 |
conj |
Er81,RSV |
R5946 |
T13317 |
T13316 |
punct |
-,Er81 |
R5947 |
T13318 |
T13316 |
punct |
", ",Er81 |
R5948 |
T13319 |
T13316 |
cc |
or,Er81 |
R5949 |
T13320 |
T13321 |
compound |
pRc,RSV |
R5950 |
T13321 |
T13323 |
compound |
RSV,Pea3 |
R5951 |
T13322 |
T13321 |
punct |
/,RSV |
R5952 |
T13323 |
T13316 |
conj |
Pea3,Er81 |
R5953 |
T13324 |
T13323 |
punct |
-,Pea3 |
R5954 |
T13325 |
T13323 |
compound |
EWS,Pea3 |
R5955 |
T13326 |
T13323 |
punct |
-,Pea3 |
R5956 |
T13327 |
T13302 |
punct |
;,one |
R5957 |
T13328 |
T13302 |
conj |
one,one |
R5958 |
T13329 |
T13328 |
prep |
of,one |
R5959 |
T13330 |
T13331 |
det |
the,plasmids |
R5960 |
T13331 |
T13329 |
pobj |
plasmids,of |
R5961 |
T13332 |
T13331 |
compound |
reporter,plasmids |
R5962 |
T13333 |
T13334 |
compound |
pTP,5xETS |
R5963 |
T13334 |
T13328 |
appos |
5xETS,one |
R5964 |
T13335 |
T13334 |
punct |
-,5xETS |
R5965 |
T13336 |
T13334 |
cc |
or,5xETS |
R5966 |
T13337 |
T13338 |
compound |
pTP,5xETSmut |
R5967 |
T13338 |
T13334 |
conj |
5xETSmut,5xETS |
R5968 |
T13339 |
T13338 |
punct |
-,5xETSmut |
R5969 |
T13340 |
T13328 |
punct |
;,one |
R5970 |
T13341 |
T13328 |
cc |
and,one |
R5971 |
T13342 |
T13328 |
conj |
placZ,one |
R5972 |
T13343 |
T13291 |
punct |
.,co-transfected |
R5973 |
T13345 |
T13346 |
nsubjpass |
Cells,harvested |
R5974 |
T13347 |
T13346 |
auxpass |
were,harvested |
R5975 |
T13348 |
T13346 |
prep |
after,harvested |
R5976 |
T13349 |
T13350 |
nummod |
25,h |
R5977 |
T13350 |
T13348 |
pobj |
h,after |
R5978 |
T13351 |
T13346 |
cc |
and,harvested |
R5979 |
T13352 |
T13346 |
conj |
processed,harvested |
R5980 |
T13353 |
T13352 |
prep |
for,processed |
R5981 |
T13354 |
T13353 |
pobj |
assays,for |
R5982 |
T13355 |
T13356 |
aux |
to,determine |
R5983 |
T13356 |
T13352 |
advcl |
determine,processed |
R5984 |
T13357 |
T13358 |
nmod |
luciferase,activity |
R5985 |
T13358 |
T13356 |
dobj |
activity,determine |
R5986 |
T13359 |
T13357 |
cc |
and,luciferase |
R5987 |
T13360 |
T13357 |
conj |
LacZ,luciferase |
R5988 |
T13361 |
T13362 |
mark |
as,described |
R5989 |
T13362 |
T13356 |
advcl |
described,determine |
R5990 |
T13363 |
T13362 |
advmod |
previously,described |
R5991 |
T13364 |
T13365 |
punct |
[,44 |
R5992 |
T13365 |
T13352 |
parataxis |
44,processed |
R5993 |
T13366 |
T13365 |
punct |
],44 |
R5994 |
T13367 |
T13346 |
punct |
.,harvested |
R5995 |
T13369 |
T13370 |
compound |
Luciferase,values |
R5996 |
T13370 |
T13371 |
nsubjpass |
values,referred |
R5997 |
T13372 |
T13370 |
acl |
normalized,values |
R5998 |
T13373 |
T13372 |
prep |
to,normalized |
R5999 |
T13374 |
T13375 |
compound |
LacZ,activity |
R6000 |
T13375 |
T13373 |
pobj |
activity,to |
R6001 |
T13376 |
T13371 |
auxpass |
are,referred |
R6002 |
T13377 |
T13371 |
prep |
to,referred |
R6003 |
T13378 |
T13371 |
prep |
as,referred |
R6004 |
T13379 |
T13380 |
compound |
luciferase,units |
R6005 |
T13380 |
T13378 |
pobj |
units,as |
R6006 |
T13381 |
T13371 |
punct |
.,referred |
R6013 |
T13917 |
T13918 |
advmod |
In,situ |
R6014 |
T13918 |
T13919 |
amod |
situ,hybridization |
R6015 |
T13920 |
T13919 |
cc |
and,hybridization |
R6016 |
T13921 |
T13919 |
conj |
immunohistochemistry,hybridization |
R6017 |
T13923 |
T13924 |
prep |
For,hybridized |
R6018 |
T13925 |
T13926 |
advmod |
in,situ |
R6019 |
T13926 |
T13927 |
amod |
situ,analysis |
R6020 |
T13927 |
T13923 |
pobj |
analysis,For |
R6021 |
T13928 |
T13927 |
compound |
hybridization,analysis |
R6022 |
T13929 |
T13924 |
punct |
", ",hybridized |
R6023 |
T13930 |
T13931 |
compound |
cryostat,sections |
R6024 |
T13931 |
T13924 |
nsubjpass |
sections,hybridized |
R6025 |
T13932 |
T13924 |
auxpass |
were,hybridized |
R6026 |
T13933 |
T13924 |
advcl |
using,hybridized |
R6027 |
T13934 |
T13935 |
npadvmod |
digoxigenin,labeled |
R6028 |
T13935 |
T13937 |
amod |
labeled,probes |
R6029 |
T13936 |
T13935 |
punct |
-,labeled |
R6030 |
T13937 |
T13933 |
dobj |
probes,using |
R6031 |
T13938 |
T13939 |
punct |
[,45 |
R6032 |
T13939 |
T13937 |
parataxis |
45,probes |
R6033 |
T13940 |
T13939 |
punct |
],45 |
R6034 |
T13941 |
T13937 |
acl |
directed,probes |
R6035 |
T13942 |
T13941 |
prep |
against,directed |
R6036 |
T13943 |
T13944 |
compound |
mouse,TrkA |
R6037 |
T13944 |
T13942 |
pobj |
TrkA,against |
R6038 |
T13945 |
T13944 |
cc |
or,TrkA |
R6039 |
T13946 |
T13944 |
conj |
TrkB,TrkA |
R6040 |
T13947 |
T13944 |
punct |
", ",TrkA |
R6041 |
T13948 |
T13944 |
cc |
or,TrkA |
R6042 |
T13949 |
T13950 |
compound |
rat,TrkC |
R6043 |
T13950 |
T13944 |
conj |
TrkC,TrkA |
R6044 |
T13951 |
T13952 |
punct |
(,gift |
R6045 |
T13952 |
T13950 |
parataxis |
gift,TrkC |
R6046 |
T13953 |
T13952 |
prep |
from,gift |
R6047 |
T13954 |
T13955 |
compound |
L.,Parada |
R6048 |
T13955 |
T13953 |
pobj |
Parada,from |
R6049 |
T13956 |
T13955 |
compound |
F.,Parada |
R6050 |
T13957 |
T13952 |
punct |
),gift |
R6051 |
T13958 |
T13924 |
punct |
.,hybridized |
R6052 |
T13960 |
T13961 |
nsubj |
Antibodies,were |
R6053 |
T13962 |
T13960 |
acl |
used,Antibodies |
R6054 |
T13963 |
T13962 |
prep |
in,used |
R6055 |
T13964 |
T13965 |
det |
this,study |
R6056 |
T13965 |
T13963 |
pobj |
study,in |
R6057 |
T13966 |
T13967 |
mark |
as,follows |
R6058 |
T13967 |
T13961 |
advcl |
follows,were |
R6059 |
T13968 |
T13961 |
punct |
: ,were |
R6060 |
T13969 |
T13970 |
compound |
rabbit,anti-Er81 |
R6061 |
T13970 |
T13961 |
dep |
anti-Er81,were |
R6062 |
T13971 |
T13972 |
punct |
[,14 |
R6063 |
T13972 |
T13970 |
parataxis |
14,anti-Er81 |
R6064 |
T13973 |
T13972 |
punct |
],14 |
R6065 |
T13974 |
T13970 |
punct |
", ",anti-Er81 |
R6066 |
T13975 |
T13970 |
conj |
rabbit,anti-Er81 |
R6067 |
T13976 |
T13975 |
amod |
anti-Pea3,rabbit |
R6068 |
T13977 |
T13978 |
punct |
[,14 |
R6069 |
T13978 |
T13975 |
parataxis |
14,rabbit |
R6070 |
T13979 |
T13978 |
punct |
],14 |
R6071 |
T13980 |
T13975 |
punct |
", ",rabbit |
R6072 |
T13981 |
T13975 |
conj |
rabbit,rabbit |
R6073 |
T13982 |
T13981 |
amod |
anti-PV,rabbit |
R6074 |
T13983 |
T13984 |
punct |
[,14 |
R6075 |
T13984 |
T13981 |
parataxis |
14,rabbit |
R6076 |
T13985 |
T13984 |
punct |
],14 |
R6077 |
T13986 |
T13981 |
punct |
", ",rabbit |
R6078 |
T13987 |
T13981 |
conj |
rabbit,rabbit |
R6079 |
T13988 |
T13987 |
amod |
anti-eGFP,rabbit |
R6080 |
T13989 |
T13990 |
punct |
(,Probes |
R6081 |
T13990 |
T13987 |
parataxis |
Probes,rabbit |
R6082 |
T13991 |
T13990 |
compound |
Molecular,Probes |
R6083 |
T13992 |
T13990 |
punct |
", ",Probes |
R6084 |
T13993 |
T13990 |
npadvmod |
Eugene,Probes |
R6085 |
T13994 |
T13990 |
punct |
", ",Probes |
R6086 |
T13995 |
T13990 |
npadvmod |
Oregon,Probes |
R6087 |
T13996 |
T13990 |
punct |
", ",Probes |
R6088 |
T13997 |
T13998 |
compound |
United,States |
R6089 |
T13998 |
T13990 |
npadvmod |
States,Probes |
R6090 |
T13999 |
T13990 |
punct |
),Probes |
R6091 |
T14000 |
T13987 |
punct |
", ",rabbit |
R6092 |
T14001 |
T13987 |
conj |
rabbit,rabbit |
R6093 |
T14002 |
T14001 |
amod |
anti-Calbindin,rabbit |
R6094 |
T14003 |
T14001 |
punct |
", ",rabbit |
R6095 |
T14004 |
T14001 |
conj |
rabbit,rabbit |
R6096 |
T14005 |
T14004 |
amod |
anti-Calretinin,rabbit |
R6097 |
T14006 |
T14007 |
punct |
(,Swant |
R6098 |
T14007 |
T14004 |
parataxis |
Swant,rabbit |
R6099 |
T14008 |
T14007 |
punct |
", ",Swant |
R6100 |
T14009 |
T14007 |
npadvmod |
Bellinzona,Swant |
R6101 |
T14010 |
T14007 |
punct |
", ",Swant |
R6102 |
T14011 |
T14007 |
npadvmod |
Switzerland,Swant |
R6103 |
T14012 |
T14007 |
punct |
),Swant |
R6104 |
T14013 |
T14004 |
punct |
", ",rabbit |
R6105 |
T14014 |
T14004 |
conj |
rabbit,rabbit |
R6106 |
T14015 |
T14014 |
amod |
anti-CGRP,rabbit |
R6107 |
T14016 |
T14017 |
punct |
(,Chemicon |
R6108 |
T14017 |
T14014 |
parataxis |
Chemicon,rabbit |
R6109 |
T14018 |
T14017 |
punct |
", ",Chemicon |
R6110 |
T14019 |
T14017 |
npadvmod |
Temecula,Chemicon |
R6111 |
T14020 |
T14017 |
punct |
", ",Chemicon |
R6112 |
T14021 |
T14017 |
npadvmod |
California,Chemicon |
R6113 |
T14022 |
T14017 |
punct |
", ",Chemicon |
R6114 |
T14023 |
T14024 |
compound |
United,States |
R6115 |
T14024 |
T14017 |
npadvmod |
States,Chemicon |
R6116 |
T14025 |
T14017 |
punct |
),Chemicon |
R6117 |
T14026 |
T14014 |
punct |
", ",rabbit |
R6118 |
T14027 |
T14014 |
conj |
rabbit,rabbit |
R6119 |
T14028 |
T14027 |
amod |
anti-vGlut1,rabbit |
R6120 |
T14029 |
T14030 |
punct |
(,Systems |
R6121 |
T14030 |
T14027 |
parataxis |
Systems,rabbit |
R6122 |
T14031 |
T14030 |
compound |
Synaptic,Systems |
R6123 |
T14032 |
T14030 |
punct |
", ",Systems |
R6124 |
T14033 |
T14030 |
npadvmod |
Goettingen,Systems |
R6125 |
T14034 |
T14030 |
punct |
", ",Systems |
R6126 |
T14035 |
T14030 |
npadvmod |
Germany,Systems |
R6127 |
T14036 |
T14030 |
punct |
),Systems |
R6128 |
T14037 |
T14027 |
punct |
", ",rabbit |
R6129 |
T14038 |
T14027 |
conj |
rabbit,rabbit |
R6130 |
T14039 |
T14038 |
amod |
anti-Brn3a,rabbit |
R6131 |
T14040 |
T14041 |
punct |
(,gift |
R6132 |
T14041 |
T14038 |
parataxis |
gift,rabbit |
R6133 |
T14042 |
T14041 |
prep |
from,gift |
R6134 |
T14043 |
T14044 |
compound |
E.,Turner |
R6135 |
T14044 |
T14042 |
pobj |
Turner,from |
R6136 |
T14045 |
T14041 |
punct |
),gift |
R6137 |
T14046 |
T14038 |
punct |
", ",rabbit |
R6138 |
T14047 |
T14048 |
nmod |
rabbit,p75 |
R6139 |
T14048 |
T14038 |
conj |
p75,rabbit |
R6140 |
T14049 |
T14048 |
amod |
anti-TrkA,p75 |
R6141 |
T14050 |
T14048 |
cc |
and,p75 |
R6142 |
T14051 |
T14048 |
punct |
-,p75 |
R6143 |
T14052 |
T14053 |
punct |
(,gift |
R6144 |
T14053 |
T14048 |
parataxis |
gift,p75 |
R6145 |
T14054 |
T14053 |
prep |
from,gift |
R6146 |
T14055 |
T14056 |
compound |
L.,Reichardt |
R6147 |
T14056 |
T14054 |
pobj |
Reichardt,from |
R6148 |
T14057 |
T14056 |
compound |
F.,Reichardt |
R6149 |
T14058 |
T14053 |
punct |
),gift |
R6150 |
T14059 |
T14048 |
punct |
", ",p75 |
R6151 |
T14060 |
T14048 |
conj |
rabbit,p75 |
R6152 |
T14061 |
T14060 |
amod |
anti-Runx3,rabbit |
R6153 |
T14062 |
T14063 |
punct |
(,Kramer |
R6154 |
T14063 |
T14060 |
parataxis |
Kramer,rabbit |
R6155 |
T14064 |
T14063 |
cc |
and,Kramer |
R6156 |
T14065 |
T14063 |
conj |
Arber,Kramer |
R6157 |
T14066 |
T14063 |
punct |
", ",Kramer |
R6158 |
T14067 |
T14068 |
amod |
unpublished,reagent |
R6159 |
T14068 |
T14063 |
npadvmod |
reagent,Kramer |
R6160 |
T14069 |
T14063 |
punct |
),Kramer |
R6161 |
T14070 |
T14060 |
punct |
", ",rabbit |
R6162 |
T14071 |
T14060 |
conj |
rabbit,rabbit |
R6163 |
T14072 |
T14071 |
amod |
anti-Rhodamine,rabbit |
R6164 |
T14073 |
T14074 |
punct |
(,Probes |
R6165 |
T14074 |
T14071 |
parataxis |
Probes,rabbit |
R6166 |
T14075 |
T14074 |
compound |
Molecular,Probes |
R6167 |
T14076 |
T14074 |
punct |
),Probes |
R6168 |
T14077 |
T14071 |
punct |
", ",rabbit |
R6169 |
T14078 |
T14071 |
conj |
mouse,rabbit |
R6170 |
T14079 |
T14078 |
amod |
anti-neurofilament,mouse |
R6171 |
T14080 |
T14081 |
punct |
(,Collection |
R6172 |
T14081 |
T14078 |
parataxis |
Collection,mouse |
R6173 |
T14082 |
T14081 |
compound |
American,Collection |
R6174 |
T14083 |
T14081 |
compound |
Type,Collection |
R6175 |
T14084 |
T14081 |
compound |
Culture,Collection |
R6176 |
T14085 |
T14081 |
punct |
", ",Collection |
R6177 |
T14086 |
T14081 |
npadvmod |
Manassas,Collection |
R6178 |
T14087 |
T14081 |
punct |
", ",Collection |
R6179 |
T14088 |
T14081 |
npadvmod |
Virginia,Collection |
R6180 |
T14089 |
T14081 |
punct |
", ",Collection |
R6181 |
T14090 |
T14091 |
compound |
United,States |
R6182 |
T14091 |
T14081 |
npadvmod |
States,Collection |
R6183 |
T14092 |
T14081 |
punct |
),Collection |
R6184 |
T14093 |
T14078 |
punct |
", ",mouse |
R6185 |
T14094 |
T14078 |
conj |
sheep,mouse |
R6186 |
T14095 |
T14094 |
amod |
anti-eGFP,sheep |
R6187 |
T14096 |
T14097 |
punct |
(,Biogenesis |
R6188 |
T14097 |
T14094 |
parataxis |
Biogenesis,sheep |
R6189 |
T14098 |
T14097 |
punct |
", ",Biogenesis |
R6190 |
T14099 |
T14097 |
npadvmod |
Poole,Biogenesis |
R6191 |
T14100 |
T14097 |
punct |
", ",Biogenesis |
R6192 |
T14101 |
T14102 |
compound |
United,Kingdom |
R6193 |
T14102 |
T14097 |
npadvmod |
Kingdom,Biogenesis |
R6194 |
T14103 |
T14097 |
punct |
),Biogenesis |
R6195 |
T14104 |
T14094 |
punct |
", ",sheep |
R6196 |
T14105 |
T14094 |
conj |
goat,sheep |
R6197 |
T14106 |
T14105 |
amod |
anti-LacZ,goat |
R6198 |
T14107 |
T14108 |
punct |
[,14 |
R6199 |
T14108 |
T14105 |
parataxis |
14,goat |
R6200 |
T14109 |
T14108 |
punct |
],14 |
R6201 |
T14110 |
T14105 |
punct |
", ",goat |
R6202 |
T14111 |
T14105 |
conj |
goat,goat |
R6203 |
T14112 |
T14111 |
amod |
anti-TrkC,goat |
R6204 |
T14113 |
T14114 |
punct |
(,gift |
R6205 |
T14114 |
T14111 |
parataxis |
gift,goat |
R6206 |
T14115 |
T14114 |
prep |
from,gift |
R6207 |
T14116 |
T14117 |
compound |
L.,Reichardt |
R6208 |
T14117 |
T14115 |
pobj |
Reichardt,from |
R6209 |
T14118 |
T14117 |
compound |
F.,Reichardt |
R6210 |
T14119 |
T14114 |
punct |
),gift |
R6211 |
T14120 |
T14111 |
punct |
", ",goat |
R6212 |
T14121 |
T14111 |
cc |
and,goat |
R6213 |
T14122 |
T14123 |
compound |
guinea,pig |
R6214 |
T14123 |
T14111 |
conj |
pig,goat |
R6215 |
T14124 |
T14123 |
amod |
anti-Isl1,pig |
R6216 |
T14125 |
T14126 |
punct |
[,14 |
R6217 |
T14126 |
T14123 |
parataxis |
14,pig |
R6218 |
T14127 |
T14126 |
punct |
],14 |
R6219 |
T14128 |
T13961 |
punct |
.,were |
R6220 |
T14130 |
T14131 |
amod |
Terminal,transferase |
R6221 |
T14131 |
T14133 |
npadvmod |
transferase,mediated |
R6222 |
T14132 |
T14131 |
compound |
deoxynucleotidyl,transferase |
R6223 |
T14133 |
T14135 |
amod |
mediated,labeling |
R6224 |
T14134 |
T14133 |
punct |
-,mediated |
R6225 |
T14135 |
T14140 |
nsubjpass |
labeling,performed |
R6226 |
T14136 |
T14135 |
amod |
biotinylated,labeling |
R6227 |
T14137 |
T14135 |
compound |
UTP,labeling |
R6228 |
T14138 |
T14135 |
compound |
nick,labeling |
R6229 |
T14139 |
T14135 |
compound |
end,labeling |
R6230 |
T14141 |
T14135 |
punct |
(,labeling |
R6231 |
T14142 |
T14135 |
appos |
TUNEL,labeling |
R6232 |
T14143 |
T14135 |
punct |
),labeling |
R6233 |
T14144 |
T14145 |
aux |
to,detect |
R6234 |
T14145 |
T14135 |
advcl |
detect,labeling |
R6235 |
T14146 |
T14147 |
amod |
apoptotic,cells |
R6236 |
T14147 |
T14145 |
dobj |
cells,detect |
R6237 |
T14148 |
T14145 |
prep |
in,detect |
R6238 |
T14149 |
T14150 |
compound |
E13.5,DRG |
R6239 |
T14150 |
T14148 |
pobj |
DRG,in |
R6240 |
T14151 |
T14145 |
prep |
on,detect |
R6241 |
T14152 |
T14153 |
compound |
cryostat,sections |
R6242 |
T14153 |
T14151 |
pobj |
sections,on |
R6243 |
T14154 |
T14140 |
auxpass |
was,performed |
R6244 |
T14155 |
T14156 |
mark |
as,described |
R6245 |
T14156 |
T14140 |
advcl |
described,performed |
R6246 |
T14157 |
T14156 |
agent |
by,described |
R6247 |
T14158 |
T14159 |
det |
the,manufacturer |
R6248 |
T14159 |
T14157 |
pobj |
manufacturer,by |
R6249 |
T14160 |
T14161 |
punct |
(,Roche |
R6250 |
T14161 |
T14159 |
parataxis |
Roche,manufacturer |
R6251 |
T14162 |
T14161 |
punct |
", ",Roche |
R6252 |
T14163 |
T14161 |
npadvmod |
Basel,Roche |
R6253 |
T14164 |
T14161 |
punct |
", ",Roche |
R6254 |
T14165 |
T14161 |
npadvmod |
Switzerland,Roche |
R6255 |
T14166 |
T14161 |
punct |
),Roche |
R6256 |
T14167 |
T14140 |
punct |
.,performed |
R6257 |
T14169 |
T14170 |
amod |
Quantitative,analysis |
R6258 |
T14170 |
T14171 |
nsubjpass |
analysis,performed |
R6259 |
T14172 |
T14170 |
prep |
of,analysis |
R6260 |
T14173 |
T14174 |
nmod |
TUNEL,cells |
R6261 |
T14174 |
T14172 |
pobj |
cells,of |
R6262 |
T14175 |
T14173 |
punct |
+,TUNEL |
R6263 |
T14176 |
T14174 |
compound |
DRG,cells |
R6264 |
T14177 |
T14171 |
auxpass |
was,performed |
R6265 |
T14178 |
T14179 |
advmod |
essentially,described |
R6266 |
T14179 |
T14171 |
advcl |
described,performed |
R6267 |
T14180 |
T14179 |
mark |
as,described |
R6268 |
T14181 |
T14182 |
punct |
[,27 |
R6269 |
T14182 |
T14179 |
parataxis |
27,described |
R6270 |
T14183 |
T14182 |
punct |
],27 |
R6271 |
T14184 |
T14171 |
punct |
.,performed |
R6272 |
T14186 |
T14187 |
compound |
BrdU,experiments |
R6273 |
T14187 |
T14191 |
nsubjpass |
experiments,performed |
R6274 |
T14188 |
T14189 |
compound |
pulse,chase |
R6275 |
T14189 |
T14187 |
compound |
chase,experiments |
R6276 |
T14190 |
T14189 |
punct |
-,chase |
R6277 |
T14192 |
T14187 |
cc |
and,experiments |
R6278 |
T14193 |
T14194 |
compound |
LacZ,stainings |
R6279 |
T14194 |
T14187 |
conj |
stainings,experiments |
R6280 |
T14195 |
T14194 |
compound |
wholemount,stainings |
R6281 |
T14196 |
T14191 |
auxpass |
were,performed |
R6282 |
T14197 |
T14198 |
mark |
as,described |
R6283 |
T14198 |
T14191 |
advcl |
described,performed |
R6284 |
T14199 |
T14198 |
advmod |
previously,described |
R6285 |
T14200 |
T14201 |
punct |
[,46 |
R6286 |
T14201 |
T14198 |
parataxis |
46,described |
R6287 |
T14202 |
T14201 |
punct |
],46 |
R6288 |
T14203 |
T14191 |
punct |
.,performed |
R6289 |
T14205 |
T14206 |
mark |
For,visualize |
R6290 |
T14206 |
T14211 |
advcl |
visualize,injected |
R6291 |
T14207 |
T14208 |
amod |
anterograde,experiments |
R6292 |
T14208 |
T14206 |
nsubj |
experiments,visualize |
R6293 |
T14209 |
T14208 |
compound |
tracing,experiments |
R6294 |
T14210 |
T14206 |
aux |
to,visualize |
R6295 |
T14212 |
T14206 |
dobj |
projections,visualize |
R6296 |
T14213 |
T14212 |
prep |
of,projections |
R6297 |
T14214 |
T14215 |
amod |
sensory,neurons |
R6298 |
T14215 |
T14213 |
pobj |
neurons,of |
R6299 |
T14216 |
T14211 |
punct |
", ",injected |
R6300 |
T14217 |
T14218 |
npadvmod |
rhodamine,conjugated |
R6301 |
T14218 |
T14220 |
amod |
conjugated,dextran |
R6302 |
T14219 |
T14218 |
punct |
-,conjugated |
R6303 |
T14220 |
T14211 |
nsubjpass |
dextran,injected |
R6304 |
T14221 |
T14222 |
punct |
(,Probes |
R6305 |
T14222 |
T14220 |
parataxis |
Probes,dextran |
R6306 |
T14223 |
T14222 |
compound |
Molecular,Probes |
R6307 |
T14224 |
T14222 |
punct |
),Probes |
R6308 |
T14225 |
T14211 |
auxpass |
was,injected |
R6309 |
T14226 |
T14211 |
prep |
into,injected |
R6310 |
T14227 |
T14228 |
amod |
single,DRG |
R6311 |
T14228 |
T14226 |
pobj |
DRG,into |
R6312 |
T14229 |
T14228 |
nmod |
lumbar,DRG |
R6313 |
T14230 |
T14231 |
punct |
(,L3 |
R6314 |
T14231 |
T14228 |
parataxis |
L3,DRG |
R6315 |
T14232 |
T14231 |
punct |
),L3 |
R6316 |
T14233 |
T14211 |
prep |
at,injected |
R6317 |
T14234 |
T14233 |
pobj |
E13.5,at |
R6318 |
T14235 |
T14211 |
cc |
or,injected |
R6319 |
T14236 |
T14211 |
conj |
applied,injected |
R6320 |
T14237 |
T14236 |
prep |
to,applied |
R6321 |
T14238 |
T14239 |
amod |
whole,roots |
R6322 |
T14239 |
T14237 |
pobj |
roots,to |
R6323 |
T14240 |
T14239 |
amod |
lumbar,roots |
R6324 |
T14241 |
T14239 |
amod |
dorsal,roots |
R6325 |
T14242 |
T14243 |
punct |
(,L3 |
R6326 |
T14243 |
T14239 |
parataxis |
L3,roots |
R6327 |
T14244 |
T14243 |
punct |
),L3 |
R6328 |
T14245 |
T14236 |
prep |
at,applied |
R6329 |
T14246 |
T14247 |
amod |
postnatal,day |
R6330 |
T14247 |
T14245 |
pobj |
day,at |
R6331 |
T14248 |
T14247 |
punct |
(,day |
R6332 |
T14249 |
T14247 |
appos |
P,day |
R6333 |
T14250 |
T14247 |
punct |
),day |
R6334 |
T14251 |
T14247 |
nummod |
5,day |
R6335 |
T14252 |
T14236 |
advcl |
using,applied |
R6336 |
T14253 |
T14254 |
compound |
glass,capillaries |
R6337 |
T14254 |
T14252 |
dobj |
capillaries,using |
R6338 |
T14255 |
T14211 |
punct |
.,injected |
R6339 |
T14257 |
T14258 |
prep |
After,incubated |
R6340 |
T14259 |
T14257 |
pobj |
injection,After |
R6341 |
T14260 |
T14258 |
punct |
", ",incubated |
R6342 |
T14261 |
T14258 |
nsubjpass |
animals,incubated |
R6343 |
T14262 |
T14258 |
auxpass |
were,incubated |
R6344 |
T14263 |
T14258 |
prep |
for,incubated |
R6345 |
T14264 |
T14265 |
quantmod |
2,3 |
R6346 |
T14265 |
T14267 |
nummod |
3,h |
R6347 |
T14266 |
T14265 |
punct |
–,3 |
R6348 |
T14267 |
T14263 |
pobj |
h,for |
R6349 |
T14268 |
T14269 |
punct |
(,E13.5 |
R6350 |
T14269 |
T14267 |
parataxis |
E13.5,h |
R6351 |
T14270 |
T14269 |
punct |
),E13.5 |
R6352 |
T14271 |
T14263 |
cc |
or,for |
R6353 |
T14272 |
T14263 |
conj |
overnight,for |
R6354 |
T14273 |
T14274 |
punct |
(,P5 |
R6355 |
T14274 |
T14272 |
parataxis |
P5,overnight |
R6356 |
T14275 |
T14274 |
punct |
),P5 |
R6357 |
T14276 |
T14258 |
punct |
.,incubated |
R6358 |
T14278 |
T14279 |
compound |
Cryostat,sections |
R6359 |
T14279 |
T14280 |
nsubjpass |
sections,processed |
R6360 |
T14281 |
T14280 |
auxpass |
were,processed |
R6361 |
T14282 |
T14280 |
prep |
for,processed |
R6362 |
T14283 |
T14282 |
pobj |
immunohistochemistry,for |
R6363 |
T14284 |
T14285 |
mark |
as,described |
R6364 |
T14285 |
T14280 |
advcl |
described,processed |
R6365 |
T14286 |
T14287 |
punct |
[,14 |
R6366 |
T14287 |
T14285 |
parataxis |
14,described |
R6367 |
T14288 |
T14287 |
punct |
],14 |
R6368 |
T14289 |
T14280 |
advcl |
using,processed |
R6369 |
T14290 |
T14291 |
npadvmod |
fluorophore,conjugated |
R6370 |
T14291 |
T14293 |
amod |
conjugated,antibodies |
R6371 |
T14292 |
T14291 |
punct |
-,conjugated |
R6372 |
T14293 |
T14289 |
dobj |
antibodies,using |
R6373 |
T14294 |
T14293 |
amod |
secondary,antibodies |
R6374 |
T14295 |
T14296 |
punct |
(,Probes |
R6375 |
T14296 |
T14293 |
parataxis |
Probes,antibodies |
R6376 |
T14297 |
T14296 |
dep |
1,Probes |
R6377 |
T14298 |
T14299 |
punct |
:,"1,000" |
R6378 |
T14299 |
T14297 |
prep |
"1,000",1 |
R6379 |
T14300 |
T14296 |
punct |
", ",Probes |
R6380 |
T14301 |
T14296 |
compound |
Molecular,Probes |
R6381 |
T14302 |
T14296 |
punct |
),Probes |
R6382 |
T14303 |
T14280 |
punct |
.,processed |
R6383 |
T14305 |
T14306 |
nsubjpass |
Images,collected |
R6384 |
T14307 |
T14306 |
auxpass |
were,collected |
R6385 |
T14308 |
T14306 |
prep |
on,collected |
R6386 |
T14309 |
T14310 |
det |
an,microscope |
R6387 |
T14310 |
T14308 |
pobj |
microscope,on |
R6388 |
T14311 |
T14310 |
nmod |
Olympus,microscope |
R6389 |
T14312 |
T14313 |
punct |
(,Tokyo |
R6390 |
T14313 |
T14311 |
parataxis |
Tokyo,Olympus |
R6391 |
T14314 |
T14313 |
punct |
", ",Tokyo |
R6392 |
T14315 |
T14313 |
npadvmod |
Japan,Tokyo |
R6393 |
T14316 |
T14313 |
punct |
),Tokyo |
R6394 |
T14317 |
T14310 |
amod |
confocal,microscope |
R6395 |
T14318 |
T14306 |
punct |
.,collected |
R6396 |
T14320 |
T14321 |
nsubjpass |
Images,collected |
R6397 |
T14322 |
T14320 |
prep |
from,Images |
R6398 |
T14323 |
T14324 |
nmod |
in,situ |
R6399 |
T14324 |
T14325 |
nmod |
situ,experiments |
R6400 |
T14325 |
T14322 |
pobj |
experiments,from |
R6401 |
T14326 |
T14325 |
compound |
hybridization,experiments |
R6402 |
T14327 |
T14321 |
auxpass |
were,collected |
R6403 |
T14328 |
T14321 |
prep |
with,collected |
R6404 |
T14329 |
T14330 |
det |
an,camera |
R6405 |
T14330 |
T14328 |
pobj |
camera,with |
R6406 |
T14331 |
T14332 |
compound |
RT,SPOT |
R6407 |
T14332 |
T14330 |
compound |
SPOT,camera |
R6408 |
T14333 |
T14332 |
punct |
-,SPOT |
R6409 |
T14334 |
T14335 |
punct |
(,Instruments |
R6410 |
T14335 |
T14330 |
parataxis |
Instruments,camera |
R6411 |
T14336 |
T14335 |
amod |
Diagnostic,Instruments |
R6412 |
T14337 |
T14335 |
punct |
", ",Instruments |
R6413 |
T14338 |
T14339 |
compound |
Sterling,Heights |
R6414 |
T14339 |
T14335 |
npadvmod |
Heights,Instruments |
R6415 |
T14340 |
T14335 |
punct |
", ",Instruments |
R6416 |
T14341 |
T14335 |
npadvmod |
Michigan,Instruments |
R6417 |
T14342 |
T14335 |
punct |
", ",Instruments |
R6418 |
T14343 |
T14344 |
compound |
United,States |
R6419 |
T14344 |
T14335 |
npadvmod |
States,Instruments |
R6420 |
T14345 |
T14335 |
punct |
),Instruments |
R6421 |
T14346 |
T14321 |
punct |
", ",collected |
R6422 |
T14347 |
T14321 |
cc |
and,collected |
R6423 |
T14348 |
T14349 |
nmod |
Corel,Paint |
R6424 |
T14349 |
T14360 |
nsubjpass |
Paint,used |
R6425 |
T14350 |
T14351 |
punct |
(,Prairie |
R6426 |
T14351 |
T14348 |
parataxis |
Prairie,Corel |
R6427 |
T14352 |
T14351 |
compound |
Eden,Prairie |
R6428 |
T14353 |
T14351 |
punct |
", ",Prairie |
R6429 |
T14354 |
T14351 |
npadvmod |
Minnesota,Prairie |
R6430 |
T14355 |
T14351 |
punct |
", ",Prairie |
R6431 |
T14356 |
T14357 |
compound |
United,States |
R6432 |
T14357 |
T14351 |
npadvmod |
States,Prairie |
R6433 |
T14358 |
T14351 |
punct |
),Prairie |
R6434 |
T14359 |
T14349 |
compound |
Photo,Paint |
R6435 |
T14360 |
T14321 |
conj |
used,collected |
R6436 |
T14361 |
T14349 |
nummod |
10.0,Paint |
R6437 |
T14362 |
T14360 |
auxpass |
was,used |
R6438 |
T14363 |
T14360 |
prep |
for,used |
R6439 |
T14364 |
T14365 |
amod |
digital,processing |
R6440 |
T14365 |
T14363 |
pobj |
processing,for |
R6441 |
T14366 |
T14365 |
prep |
of,processing |
R6442 |
T14367 |
T14366 |
pobj |
images,of |
R6443 |
T14368 |
T14360 |
punct |
.,used |
R6444 |
T14469 |
T14470 |
advmod |
In,vitro |
R6445 |
T14470 |
T14471 |
amod |
vitro,cultures |
R6446 |
T14472 |
T14471 |
prep |
of,cultures |
R6447 |
T14473 |
T14472 |
pobj |
DRG,of |
R6448 |
T14475 |
T14476 |
amod |
Individual,DRG |
R6449 |
T14476 |
T14478 |
nsubjpass |
DRG,dissected |
R6450 |
T14477 |
T14476 |
compound |
lumbar,DRG |
R6451 |
T14479 |
T14478 |
auxpass |
were,dissected |
R6452 |
T14480 |
T14478 |
prep |
from,dissected |
R6453 |
T14481 |
T14482 |
nmod |
E13.5,embryos |
R6454 |
T14482 |
T14480 |
pobj |
embryos,from |
R6455 |
T14483 |
T14481 |
cc |
or,E13.5 |
R6456 |
T14484 |
T14481 |
conj |
E14.5,E13.5 |
R6457 |
T14485 |
T14478 |
cc |
and,dissected |
R6458 |
T14486 |
T14478 |
conj |
placed,dissected |
R6459 |
T14487 |
T14486 |
prep |
on,placed |
R6460 |
T14488 |
T14489 |
npadvmod |
Matrigel,coated |
R6461 |
T14489 |
T14502 |
amod |
coated,coverslips |
R6462 |
T14490 |
T14491 |
punct |
(,Biosciences |
R6463 |
T14491 |
T14488 |
parataxis |
Biosciences,Matrigel |
R6464 |
T14492 |
T14491 |
compound |
BD,Biosciences |
R6465 |
T14493 |
T14491 |
punct |
", ",Biosciences |
R6466 |
T14494 |
T14495 |
compound |
San,Jose |
R6467 |
T14495 |
T14491 |
npadvmod |
Jose,Biosciences |
R6468 |
T14496 |
T14491 |
punct |
", ",Biosciences |
R6469 |
T14497 |
T14491 |
npadvmod |
California,Biosciences |
R6470 |
T14498 |
T14491 |
punct |
", ",Biosciences |
R6471 |
T14499 |
T14500 |
compound |
United,States |
R6472 |
T14500 |
T14491 |
npadvmod |
States,Biosciences |
R6473 |
T14501 |
T14491 |
punct |
),Biosciences |
R6474 |
T14502 |
T14487 |
pobj |
coverslips,on |
R6475 |
T14503 |
T14502 |
prep |
in,coverslips |
R6476 |
T14504 |
T14505 |
compound |
DMEM,F12 |
R6477 |
T14505 |
T14503 |
pobj |
F12,in |
R6478 |
T14506 |
T14505 |
punct |
/,F12 |
R6479 |
T14507 |
T14508 |
punct |
(,Gibco |
R6480 |
T14508 |
T14505 |
parataxis |
Gibco,F12 |
R6481 |
T14509 |
T14508 |
punct |
", ",Gibco |
R6482 |
T14510 |
T14511 |
compound |
San,Diego |
R6483 |
T14511 |
T14508 |
npadvmod |
Diego,Gibco |
R6484 |
T14512 |
T14508 |
punct |
", ",Gibco |
R6485 |
T14513 |
T14508 |
npadvmod |
California,Gibco |
R6486 |
T14514 |
T14508 |
punct |
", ",Gibco |
R6487 |
T14515 |
T14516 |
compound |
United,States |
R6488 |
T14516 |
T14508 |
npadvmod |
States,Gibco |
R6489 |
T14517 |
T14508 |
punct |
),Gibco |
R6490 |
T14518 |
T14502 |
punct |
", ",coverslips |
R6491 |
T14519 |
T14520 |
nummod |
2,mM |
R6492 |
T14520 |
T14521 |
compound |
mM,Gln |
R6493 |
T14521 |
T14502 |
conj |
Gln,coverslips |
R6494 |
T14522 |
T14521 |
compound |
L,Gln |
R6495 |
T14523 |
T14521 |
punct |
-,Gln |
R6496 |
T14524 |
T14525 |
punct |
(,Gibco |
R6497 |
T14525 |
T14521 |
parataxis |
Gibco,Gln |
R6498 |
T14526 |
T14525 |
punct |
),Gibco |
R6499 |
T14527 |
T14521 |
punct |
", ",Gln |
R6500 |
T14528 |
T14521 |
conj |
N2,Gln |
R6501 |
T14529 |
T14530 |
punct |
(,Gibco |
R6502 |
T14530 |
T14528 |
parataxis |
Gibco,N2 |
R6503 |
T14531 |
T14530 |
punct |
),Gibco |
R6504 |
T14532 |
T14528 |
punct |
", ",N2 |
R6505 |
T14533 |
T14528 |
cc |
and,N2 |
R6506 |
T14534 |
T14535 |
nummod |
1,mg |
R6507 |
T14535 |
T14536 |
nmod |
mg,BSA |
R6508 |
T14536 |
T14528 |
conj |
BSA,N2 |
R6509 |
T14537 |
T14538 |
punct |
/,ml |
R6510 |
T14538 |
T14535 |
prep |
ml,mg |
R6511 |
T14539 |
T14540 |
punct |
(,Sigma |
R6512 |
T14540 |
T14536 |
parataxis |
Sigma,BSA |
R6513 |
T14541 |
T14540 |
punct |
", ",Sigma |
R6514 |
T14542 |
T14543 |
compound |
St.,Louis |
R6515 |
T14543 |
T14540 |
npadvmod |
Louis,Sigma |
R6516 |
T14544 |
T14540 |
punct |
", ",Sigma |
R6517 |
T14545 |
T14540 |
npadvmod |
Missouri,Sigma |
R6518 |
T14546 |
T14540 |
punct |
", ",Sigma |
R6519 |
T14547 |
T14548 |
compound |
United,States |
R6520 |
T14548 |
T14540 |
npadvmod |
States,Sigma |
R6521 |
T14549 |
T14540 |
punct |
),Sigma |
R6522 |
T14550 |
T14536 |
prep |
without,BSA |
R6523 |
T14551 |
T14550 |
pobj |
neurotrophins,without |
R6524 |
T14552 |
T14478 |
punct |
", ",dissected |
R6525 |
T14553 |
T14478 |
cc |
or,dissected |
R6526 |
T14554 |
T14478 |
conj |
supplemented,dissected |
R6527 |
T14555 |
T14554 |
prep |
with,supplemented |
R6528 |
T14556 |
T14557 |
preconj |
either,NGF |
R6529 |
T14557 |
T14555 |
pobj |
NGF,with |
R6530 |
T14558 |
T14559 |
punct |
(,Gibco |
R6531 |
T14559 |
T14557 |
parataxis |
Gibco,NGF |
R6532 |
T14560 |
T14561 |
nummod |
100,ng |
R6533 |
T14561 |
T14559 |
dep |
ng,Gibco |
R6534 |
T14562 |
T14563 |
punct |
/,ml |
R6535 |
T14563 |
T14561 |
prep |
ml,ng |
R6536 |
T14564 |
T14559 |
punct |
", ",Gibco |
R6537 |
T14565 |
T14559 |
punct |
),Gibco |
R6538 |
T14566 |
T14557 |
cc |
or,NGF |
R6539 |
T14567 |
T14557 |
conj |
NT,NGF |
R6540 |
T14568 |
T14567 |
punct |
-,NT |
R6541 |
T14569 |
T14567 |
nummod |
3,NT |
R6542 |
T14570 |
T14571 |
punct |
(,Sigma |
R6543 |
T14571 |
T14567 |
parataxis |
Sigma,NT |
R6544 |
T14572 |
T14573 |
nummod |
20,ng |
R6545 |
T14573 |
T14571 |
dep |
ng,Sigma |
R6546 |
T14574 |
T14575 |
punct |
/,ml |
R6547 |
T14575 |
T14573 |
prep |
ml,ng |
R6548 |
T14576 |
T14571 |
punct |
", ",Sigma |
R6549 |
T14577 |
T14571 |
punct |
),Sigma |
R6550 |
T14578 |
T14478 |
punct |
.,dissected |
R6551 |
T14580 |
T14581 |
compound |
DRG,explants |
R6552 |
T14581 |
T14582 |
nsubjpass |
explants,cultured |
R6553 |
T14583 |
T14584 |
punct |
(,20 |
R6554 |
T14584 |
T14581 |
parataxis |
20,explants |
R6555 |
T14585 |
T14584 |
nsubj |
n,20 |
R6556 |
T14586 |
T14584 |
punct |
≥,20 |
R6557 |
T14587 |
T14584 |
prep |
for,20 |
R6558 |
T14588 |
T14589 |
det |
each,condition |
R6559 |
T14589 |
T14587 |
pobj |
condition,for |
R6560 |
T14590 |
T14584 |
punct |
),20 |
R6561 |
T14591 |
T14582 |
auxpass |
were,cultured |
R6562 |
T14592 |
T14582 |
prep |
for,cultured |
R6563 |
T14593 |
T14594 |
nummod |
48,h |
R6564 |
T14594 |
T14592 |
pobj |
h,for |
R6565 |
T14595 |
T14582 |
punct |
", ",cultured |
R6566 |
T14596 |
T14582 |
conj |
processed,cultured |
R6567 |
T14597 |
T14596 |
prep |
for,processed |
R6568 |
T14598 |
T14597 |
pobj |
immunocytochemistry,for |
R6569 |
T14599 |
T14596 |
punct |
", ",processed |
R6570 |
T14600 |
T14596 |
cc |
and,processed |
R6571 |
T14601 |
T14596 |
conj |
analyzed,processed |
R6572 |
T14602 |
T14601 |
advcl |
using,analyzed |
R6573 |
T14603 |
T14604 |
amod |
confocal,microscopy |
R6574 |
T14604 |
T14602 |
dobj |
microscopy,using |
R6575 |
T14605 |
T14582 |
punct |
.,cultured |
R6577 |
T14754 |
T14755 |
compound |
Western,blot |
R6578 |
T14755 |
T14756 |
compound |
blot,analysis |
R6579 |
T14758 |
T14759 |
compound |
Lumbar,DRG |
R6580 |
T14759 |
T14760 |
nsubjpass |
DRG,isolated |
R6581 |
T14761 |
T14759 |
prep |
from,DRG |
R6582 |
T14762 |
T14763 |
compound |
E16.5,embryos |
R6583 |
T14763 |
T14761 |
pobj |
embryos,from |
R6584 |
T14764 |
T14760 |
auxpass |
were,isolated |
R6585 |
T14765 |
T14760 |
punct |
", ",isolated |
R6586 |
T14766 |
T14767 |
advmod |
mechanically,disrupted |
R6587 |
T14767 |
T14760 |
conj |
disrupted,isolated |
R6588 |
T14768 |
T14767 |
punct |
", ",disrupted |
R6589 |
T14769 |
T14767 |
conj |
homogenized,disrupted |
R6590 |
T14770 |
T14769 |
advcl |
using,homogenized |
R6591 |
T14771 |
T14772 |
compound |
glass,beads |
R6592 |
T14772 |
T14770 |
dobj |
beads,using |
R6593 |
T14773 |
T14774 |
punct |
(,Sigma |
R6594 |
T14774 |
T14772 |
parataxis |
Sigma,beads |
R6595 |
T14775 |
T14774 |
punct |
),Sigma |
R6596 |
T14776 |
T14769 |
punct |
", ",homogenized |
R6597 |
T14777 |
T14769 |
cc |
and,homogenized |
R6598 |
T14778 |
T14769 |
conj |
lysed,homogenized |
R6599 |
T14779 |
T14778 |
prep |
in,lysed |
R6600 |
T14780 |
T14781 |
amod |
standard,buffer |
R6601 |
T14781 |
T14779 |
pobj |
buffer,in |
R6602 |
T14782 |
T14781 |
compound |
lysis,buffer |
R6603 |
T14783 |
T14781 |
acl |
supplemented,buffer |
R6604 |
T14784 |
T14783 |
prep |
with,supplemented |
R6605 |
T14785 |
T14786 |
nmod |
protease,inhibitors |
R6606 |
T14786 |
T14784 |
pobj |
inhibitors,with |
R6607 |
T14787 |
T14785 |
cc |
and,protease |
R6608 |
T14788 |
T14785 |
conj |
phosphatase,protease |
R6609 |
T14789 |
T14790 |
mark |
as,described |
R6610 |
T14790 |
T14783 |
advcl |
described,supplemented |
R6611 |
T14791 |
T14792 |
punct |
[,47 |
R6612 |
T14792 |
T14790 |
parataxis |
47,described |
R6613 |
T14793 |
T14792 |
punct |
],47 |
R6614 |
T14794 |
T14760 |
punct |
.,isolated |
R6615 |
T14796 |
T14797 |
compound |
Protein,extracts |
R6616 |
T14797 |
T14798 |
nsubjpass |
extracts,resolved |
R6617 |
T14799 |
T14798 |
auxpass |
were,resolved |
R6618 |
T14800 |
T14798 |
agent |
by,resolved |
R6619 |
T14801 |
T14802 |
compound |
SDS,PAGE |
R6620 |
T14802 |
T14800 |
pobj |
PAGE,by |
R6621 |
T14803 |
T14802 |
punct |
-,PAGE |
R6622 |
T14804 |
T14798 |
punct |
", ",resolved |
R6623 |
T14805 |
T14798 |
cc |
and,resolved |
R6624 |
T14806 |
T14807 |
nsubjpass |
immunoblotting,performed |
R6625 |
T14807 |
T14798 |
conj |
performed,resolved |
R6626 |
T14808 |
T14807 |
auxpass |
was,performed |
R6627 |
T14809 |
T14807 |
advcl |
using,performed |
R6628 |
T14810 |
T14809 |
dobj |
antibodies,using |
R6629 |
T14811 |
T14810 |
prep |
against,antibodies |
R6630 |
T14812 |
T14811 |
pobj |
Akt,against |
R6631 |
T14813 |
T14812 |
punct |
", ",Akt |
R6632 |
T14814 |
T14815 |
compound |
p,Akt |
R6633 |
T14815 |
T14812 |
conj |
Akt,Akt |
R6634 |
T14816 |
T14815 |
punct |
-,Akt |
R6635 |
T14817 |
T14818 |
punct |
(,Ser473 |
R6636 |
T14818 |
T14815 |
parataxis |
Ser473,Akt |
R6637 |
T14819 |
T14818 |
punct |
),Ser473 |
R6638 |
T14820 |
T14815 |
punct |
", ",Akt |
R6639 |
T14821 |
T14815 |
conj |
CREB,Akt |
R6640 |
T14822 |
T14821 |
punct |
", ",CREB |
R6641 |
T14823 |
T14824 |
compound |
p,CREB |
R6642 |
T14824 |
T14821 |
conj |
CREB,CREB |
R6643 |
T14825 |
T14824 |
punct |
-,CREB |
R6644 |
T14826 |
T14827 |
punct |
(,Ser133 |
R6645 |
T14827 |
T14824 |
parataxis |
Ser133,CREB |
R6646 |
T14828 |
T14827 |
punct |
),Ser133 |
R6647 |
T14829 |
T14824 |
punct |
", ",CREB |
R6648 |
T14830 |
T14824 |
conj |
Bax,CREB |
R6649 |
T14831 |
T14830 |
punct |
", ",Bax |
R6650 |
T14832 |
T14833 |
compound |
Bcl,xl |
R6651 |
T14833 |
T14830 |
conj |
xl,Bax |
R6652 |
T14834 |
T14833 |
punct |
-,xl |
R6653 |
T14835 |
T14836 |
punct |
(,Technology |
R6654 |
T14836 |
T14833 |
parataxis |
Technology,xl |
R6655 |
T14837 |
T14836 |
compound |
Cell,Technology |
R6656 |
T14838 |
T14836 |
compound |
Signaling,Technology |
R6657 |
T14839 |
T14836 |
punct |
", ",Technology |
R6658 |
T14840 |
T14836 |
npadvmod |
Beverly,Technology |
R6659 |
T14841 |
T14836 |
punct |
", ",Technology |
R6660 |
T14842 |
T14836 |
npadvmod |
Massachusetts,Technology |
R6661 |
T14843 |
T14836 |
punct |
", ",Technology |
R6662 |
T14844 |
T14845 |
compound |
United,States |
R6663 |
T14845 |
T14836 |
npadvmod |
States,Technology |
R6664 |
T14846 |
T14836 |
punct |
),Technology |
R6665 |
T14847 |
T14833 |
punct |
", ",xl |
R6666 |
T14848 |
T14833 |
cc |
and,xl |
R6667 |
T14849 |
T14833 |
conj |
Bcl2,xl |
R6668 |
T14850 |
T14851 |
punct |
(,Pharmingen |
R6669 |
T14851 |
T14849 |
parataxis |
Pharmingen,Bcl2 |
R6670 |
T14852 |
T14851 |
compound |
BD,Pharmingen |
R6671 |
T14853 |
T14851 |
punct |
", ",Pharmingen |
R6672 |
T14854 |
T14855 |
compound |
San,Diego |
R6673 |
T14855 |
T14851 |
npadvmod |
Diego,Pharmingen |
R6674 |
T14856 |
T14851 |
punct |
", ",Pharmingen |
R6675 |
T14857 |
T14851 |
npadvmod |
California,Pharmingen |
R6676 |
T14858 |
T14851 |
punct |
", ",Pharmingen |
R6677 |
T14859 |
T14860 |
compound |
United,States |
R6678 |
T14860 |
T14851 |
npadvmod |
States,Pharmingen |
R6679 |
T14861 |
T14851 |
punct |
),Pharmingen |
R6680 |
T14862 |
T14807 |
punct |
.,performed |
R6681 |
T14864 |
T14865 |
prep |
For,scanned |
R6682 |
T14866 |
T14864 |
pobj |
quantification,For |
R6683 |
T14867 |
T14865 |
punct |
", ",scanned |
R6684 |
T14868 |
T14865 |
nsubjpass |
films,scanned |
R6685 |
T14869 |
T14870 |
punct |
(,AR |
R6686 |
T14870 |
T14868 |
parataxis |
AR,films |
R6687 |
T14871 |
T14872 |
compound |
X,OMAT |
R6688 |
T14872 |
T14870 |
compound |
OMAT,AR |
R6689 |
T14873 |
T14872 |
punct |
-,OMAT |
R6690 |
T14874 |
T14870 |
punct |
", ",AR |
R6691 |
T14875 |
T14876 |
compound |
Eastman,Kodak |
R6692 |
T14876 |
T14870 |
npadvmod |
Kodak,AR |
R6693 |
T14877 |
T14870 |
punct |
", ",AR |
R6694 |
T14878 |
T14870 |
npadvmod |
Rochester,AR |
R6695 |
T14879 |
T14870 |
punct |
", ",AR |
R6696 |
T14880 |
T14881 |
compound |
New,York |
R6697 |
T14881 |
T14870 |
npadvmod |
York,AR |
R6698 |
T14882 |
T14870 |
punct |
", ",AR |
R6699 |
T14883 |
T14884 |
compound |
United,States |
R6700 |
T14884 |
T14870 |
npadvmod |
States,AR |
R6701 |
T14885 |
T14870 |
punct |
),AR |
R6702 |
T14886 |
T14865 |
auxpass |
were,scanned |
R6703 |
T14887 |
T14865 |
cc |
and,scanned |
R6704 |
T14888 |
T14889 |
nsubjpass |
densitometry,performed |
R6705 |
T14889 |
T14865 |
conj |
performed,scanned |
R6706 |
T14890 |
T14889 |
auxpass |
was,performed |
R6707 |
T14891 |
T14889 |
advcl |
using,performed |
R6708 |
T14892 |
T14891 |
dobj |
IMAGEQUANT,using |
R6709 |
T14893 |
T14892 |
nummod |
5.2,IMAGEQUANT |
R6710 |
T14894 |
T14895 |
punct |
(,Dynamics |
R6711 |
T14895 |
T14892 |
parataxis |
Dynamics,IMAGEQUANT |
R6712 |
T14896 |
T14895 |
compound |
Molecular,Dynamics |
R6713 |
T14897 |
T14895 |
punct |
", ",Dynamics |
R6714 |
T14898 |
T14895 |
npadvmod |
Amersham,Dynamics |
R6715 |
T14899 |
T14895 |
punct |
", ",Dynamics |
R6716 |
T14900 |
T14895 |
npadvmod |
Uppsala,Dynamics |
R6717 |
T14901 |
T14895 |
punct |
", ",Dynamics |
R6718 |
T14902 |
T14895 |
npadvmod |
Sweden,Dynamics |
R6719 |
T14903 |
T14895 |
punct |
),Dynamics |
R6720 |
T14904 |
T14889 |
punct |
.,performed |
R6721 |
T15022 |
T15023 |
amod |
Electrophysiological,analysis |
R6722 |
T15023 |
T15024 |
nsubjpass |
analysis,performed |
R6723 |
T15025 |
T15024 |
auxpass |
was,performed |
R6724 |
T15026 |
T15027 |
mark |
as,described |
R6725 |
T15027 |
T15024 |
advcl |
described,performed |
R6726 |
T15028 |
T15027 |
advmod |
previously,described |
R6727 |
T15029 |
T15030 |
punct |
[,48 |
R6728 |
T15030 |
T15027 |
parataxis |
48,described |
R6729 |
T15031 |
T15030 |
punct |
],48 |
R6730 |
T15032 |
T15024 |
punct |
.,performed |
R6731 |
T15034 |
T15035 |
advmod |
Briefly,made |
R6732 |
T15036 |
T15035 |
punct |
", ",made |
R6733 |
T15037 |
T15038 |
amod |
intracellular,recordings |
R6734 |
T15038 |
T15035 |
nsubjpass |
recordings,made |
R6735 |
T15039 |
T15038 |
prep |
from,recordings |
R6736 |
T15040 |
T15041 |
amod |
identified,neurons |
R6737 |
T15041 |
T15039 |
pobj |
neurons,from |
R6738 |
T15042 |
T15041 |
compound |
quadriceps,neurons |
R6739 |
T15043 |
T15041 |
compound |
motor,neurons |
R6740 |
T15044 |
T15035 |
auxpass |
were,made |
R6741 |
T15045 |
T15035 |
advcl |
using,made |
R6742 |
T15046 |
T15047 |
amod |
sharp,electrodes |
R6743 |
T15047 |
T15045 |
dobj |
electrodes,using |
R6744 |
T15048 |
T15049 |
punct |
(,MΩ |
R6745 |
T15049 |
T15045 |
parataxis |
MΩ,using |
R6746 |
T15050 |
T15051 |
quantmod |
75,120 |
R6747 |
T15051 |
T15049 |
nummod |
120,MΩ |
R6748 |
T15052 |
T15051 |
punct |
–,120 |
R6749 |
T15053 |
T15049 |
punct |
", ",MΩ |
R6750 |
T15054 |
T15055 |
compound |
3M,KCl |
R6751 |
T15055 |
T15049 |
appos |
KCl,MΩ |
R6752 |
T15056 |
T15049 |
punct |
),MΩ |
R6753 |
T15057 |
T15035 |
punct |
.,made |
R6754 |
T15059 |
T15060 |
amod |
Average,responses |
R6755 |
T15060 |
T15061 |
nsubjpass |
responses,acquired |
R6756 |
T15062 |
T15063 |
punct |
(,trials |
R6757 |
T15063 |
T15060 |
parataxis |
trials,responses |
R6758 |
T15064 |
T15065 |
quantmod |
10,20 |
R6759 |
T15065 |
T15063 |
nummod |
20,trials |
R6760 |
T15066 |
T15065 |
punct |
–,20 |
R6761 |
T15067 |
T15063 |
punct |
),trials |
R6762 |
T15068 |
T15060 |
prep |
from,responses |
R6763 |
T15069 |
T15070 |
compound |
suprathreshold,stimulation |
R6764 |
T15070 |
T15068 |
pobj |
stimulation,from |
R6765 |
T15071 |
T15070 |
compound |
nerve,stimulation |
R6766 |
T15072 |
T15073 |
punct |
(,strength |
R6767 |
T15073 |
T15070 |
parataxis |
strength,stimulation |
R6768 |
T15074 |
T15073 |
nummod |
1.5,strength |
R6769 |
T15075 |
T15074 |
quantmod |
times,1.5 |
R6770 |
T15076 |
T15073 |
det |
the,strength |
R6771 |
T15077 |
T15078 |
dep |
that,evokes |
R6772 |
T15078 |
T15073 |
relcl |
evokes,strength |
R6773 |
T15079 |
T15080 |
amod |
maximal,response |
R6774 |
T15080 |
T15078 |
dobj |
response,evokes |
R6775 |
T15081 |
T15080 |
amod |
monosynaptic,response |
R6776 |
T15082 |
T15073 |
punct |
),strength |
R6777 |
T15083 |
T15070 |
prep |
of,stimulation |
R6778 |
T15084 |
T15085 |
det |
the,nerve |
R6779 |
T15085 |
T15083 |
pobj |
nerve,of |
R6780 |
T15086 |
T15085 |
compound |
quadriceps,nerve |
R6781 |
T15087 |
T15061 |
auxpass |
were,acquired |
R6782 |
T15088 |
T15061 |
prep |
with,acquired |
R6783 |
T15089 |
T15090 |
compound |
LTP,software |
R6784 |
T15090 |
T15088 |
pobj |
software,with |
R6785 |
T15091 |
T15092 |
punct |
[,49 |
R6786 |
T15092 |
T15061 |
parataxis |
49,acquired |
R6787 |
T15093 |
T15092 |
punct |
],49 |
R6788 |
T15094 |
T15061 |
punct |
.,acquired |
R6789 |
T15096 |
T15097 |
advmod |
Only,cells |
R6790 |
T15097 |
T15098 |
nsubjpass |
cells,considered |
R6791 |
T15099 |
T15097 |
prep |
with,cells |
R6792 |
T15100 |
T15101 |
amod |
stable,potentials |
R6793 |
T15101 |
T15099 |
pobj |
potentials,with |
R6794 |
T15102 |
T15101 |
compound |
resting,potentials |
R6795 |
T15103 |
T15104 |
advmod |
more,negative |
R6796 |
T15104 |
T15101 |
amod |
negative,potentials |
R6797 |
T15105 |
T15104 |
prep |
than,negative |
R6798 |
T15106 |
T15107 |
punct |
−,50 |
R6799 |
T15107 |
T15108 |
nummod |
50,mV |
R6800 |
T15108 |
T15105 |
pobj |
mV,than |
R6801 |
T15109 |
T15098 |
auxpass |
were,considered |
R6802 |
T15110 |
T15098 |
prep |
for,considered |
R6803 |
T15111 |
T15110 |
pobj |
analysis,for |
R6804 |
T15112 |
T15098 |
punct |
.,considered |
R6805 |
T15114 |
T15115 |
amod |
Monosynaptic,amplitudes |
R6806 |
T15115 |
T15116 |
nsubjpass |
amplitudes,determined |
R6807 |
T15117 |
T15116 |
auxpass |
were,determined |
R6808 |
T15118 |
T15116 |
advmod |
offline,determined |
R6809 |
T15119 |
T15116 |
advcl |
using,determined |
R6810 |
T15120 |
T15121 |
compound |
custom,routines |
R6811 |
T15121 |
T15119 |
dobj |
routines,using |
R6812 |
T15122 |
T15119 |
prep |
in,using |
R6813 |
T15123 |
T15124 |
det |
the,environment |
R6814 |
T15124 |
T15122 |
pobj |
environment,in |
R6815 |
T15125 |
T15124 |
compound |
Matlab,environment |
R6816 |
T15126 |
T15127 |
punct |
(,Mathworks |
R6817 |
T15127 |
T15124 |
parataxis |
Mathworks,environment |
R6818 |
T15128 |
T15127 |
det |
The,Mathworks |
R6819 |
T15129 |
T15127 |
punct |
", ",Mathworks |
R6820 |
T15130 |
T15127 |
npadvmod |
Natick,Mathworks |
R6821 |
T15131 |
T15127 |
punct |
", ",Mathworks |
R6822 |
T15132 |
T15127 |
npadvmod |
Massachusetts,Mathworks |
R6823 |
T15133 |
T15127 |
punct |
", ",Mathworks |
R6824 |
T15134 |
T15135 |
compound |
United,States |
R6825 |
T15135 |
T15127 |
npadvmod |
States,Mathworks |
R6826 |
T15136 |
T15127 |
punct |
),Mathworks |
R6827 |
T15137 |
T15138 |
mark |
as,described |
R6828 |
T15138 |
T15119 |
advcl |
described,using |
R6829 |
T15139 |
T15138 |
advmod |
previously,described |
R6830 |
T15140 |
T15141 |
punct |
[,48 |
R6831 |
T15141 |
T15138 |
parataxis |
48,described |
R6832 |
T15142 |
T15141 |
punct |
],48 |
R6833 |
T15143 |
T15116 |
punct |
.,determined |